LOCUS       DAXFHF010000001        62915 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXFHF010000001 DAXFHF010000000
VERSION     DAXFHF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 62915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62915
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.001"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(202..1041)
                     /locus_tag="ACFIRM_000001"
     CDS             complement(202..1041)
                     /locus_tag="ACFIRM_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC3027763.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWASRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1326..1401
                     /locus_tag="ACFIRM_000002"
     tRNA            1326..1401
                     /locus_tag="ACFIRM_000002"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1359..1361,aa:Arg,seq:ccg)
     gene            complement(1466..2659)
                     /locus_tag="ACFIRM_000003"
     CDS             complement(1466..2659)
                     /locus_tag="ACFIRM_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC3027764.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2679..3767)
                     /gene="trmA"
                     /locus_tag="ACFIRM_000004"
     CDS             complement(2679..3767)
                     /gene="trmA"
                     /locus_tag="ACFIRM_000004"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC3027765.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3914..5014
                     /gene="aroC"
                     /locus_tag="ACFIRM_000005"
     CDS             3914..5014
                     /gene="aroC"
                     /locus_tag="ACFIRM_000005"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC3027766.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRTAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5105..5530
                     /locus_tag="ACFIRM_000006"
     CDS             5105..5530
                     /locus_tag="ACFIRM_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC3027767.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5604..7589)
                     /gene="parE"
                     /locus_tag="ACFIRM_000007"
     CDS             complement(5604..7589)
                     /gene="parE"
                     /locus_tag="ACFIRM_000007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC3027768.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFADGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7656..8204)
                     /locus_tag="ACFIRM_000008"
     CDS             complement(7656..8204)
                     /locus_tag="ACFIRM_000008"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC3027769.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8346..9641
                     /gene="serS"
                     /locus_tag="ACFIRM_000009"
     CDS             8346..9641
                     /gene="serS"
                     /locus_tag="ACFIRM_000009"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC3027770.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHMLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9714..10712)
                     /locus_tag="ACFIRM_000010"
     CDS             complement(9714..10712)
                     /locus_tag="ACFIRM_000010"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC3027771.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10837..11913)
                     /gene="prfA"
                     /locus_tag="ACFIRM_000011"
     CDS             complement(10837..11913)
                     /gene="prfA"
                     /locus_tag="ACFIRM_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC3027772.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12003..12773)
                     /locus_tag="ACFIRM_000012"
     CDS             complement(12003..12773)
                     /locus_tag="ACFIRM_000012"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3027773.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12776..13768)
                     /locus_tag="ACFIRM_000013"
     CDS             complement(12776..13768)
                     /locus_tag="ACFIRM_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC3027774.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13835..14518)
                     /locus_tag="ACFIRM_000014"
     CDS             complement(13835..14518)
                     /locus_tag="ACFIRM_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3027775.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14707..16041)
                     /gene="glmM"
                     /locus_tag="ACFIRM_000015"
     CDS             complement(14707..16041)
                     /gene="glmM"
                     /locus_tag="ACFIRM_000015"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC3027776.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16171..17022)
                     /gene="folP"
                     /locus_tag="ACFIRM_000016"
     CDS             complement(16171..17022)
                     /gene="folP"
                     /locus_tag="ACFIRM_000016"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC3027777.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEWARVEPVLAEAAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIVLMRHLPELMAETGL
                     PLLIGVSRKRMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGVNR"
     gene            complement(17162..18055)
                     /locus_tag="ACFIRM_000017"
     CDS             complement(17162..18055)
                     /locus_tag="ACFIRM_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC3027778.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18146..20248
                     /locus_tag="ACFIRM_000018"
     CDS             18146..20248
                     /locus_tag="ACFIRM_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC3027779.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20312..21790)
                     /gene="ubiD"
                     /locus_tag="ACFIRM_000019"
     CDS             complement(20312..21790)
                     /gene="ubiD"
                     /locus_tag="ACFIRM_000019"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC3027780.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21810..22325)
                     /locus_tag="ACFIRM_000020"
     CDS             complement(21810..22325)
                     /locus_tag="ACFIRM_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC3027781.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSDQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22332..22673)
                     /locus_tag="ACFIRM_000021"
                     /pseudo
     CDS             complement(22332..22673)
                     /locus_tag="ACFIRM_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22718..22870)
                     /locus_tag="ACFIRM_000022"
     CDS             complement(22718..22870)
                     /locus_tag="ACFIRM_000022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027782.1"
                     /translation="MKPGFFGHIFLTVLSIAYPALWYSGLNLNRYGVASPCRTICTVC
                     GFAALS"
     gene            complement(22946..23029)
                     /locus_tag="ACFIRM_000023"
                     /pseudo
     CDS             complement(22946..23029)
                     /locus_tag="ACFIRM_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23032..23774)
                     /locus_tag="ACFIRM_000024"
                     /pseudo
     CDS             complement(23032..23774)
                     /locus_tag="ACFIRM_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23975..24402
                     /locus_tag="ACFIRM_000025"
                     /pseudo
     CDS             23975..24402
                     /locus_tag="ACFIRM_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            24440..25168
                     /gene="fabG"
                     /locus_tag="ACFIRM_000026"
                     /pseudo
     CDS             24440..25168
                     /gene="fabG"
                     /locus_tag="ACFIRM_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            25162..26507
                     /locus_tag="ACFIRM_000027"
                     /pseudo
     CDS             25162..26507
                     /locus_tag="ACFIRM_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1,RefSeq:WP_003762767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            26561..26636
                     /locus_tag="ACFIRM_000028"
     tRNA            26561..26636
                     /locus_tag="ACFIRM_000028"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26594..26596,aa:Ile,seq:cat)
     gene            26711..27469
                     /locus_tag="ACFIRM_000029"
     CDS             26711..27469
                     /locus_tag="ACFIRM_000029"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3027783.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27470..28534
                     /locus_tag="ACFIRM_000030"
     CDS             27470..28534
                     /locus_tag="ACFIRM_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3027784.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28768..30105
                     /locus_tag="ACFIRM_000031"
     CDS             28768..30105
                     /locus_tag="ACFIRM_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3027785.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            complement(30280..31292)
                     /locus_tag="ACFIRM_000032"
                     /pseudo
     CDS             complement(30280..31292)
                     /locus_tag="ACFIRM_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(31301..32095)
                     /locus_tag="ACFIRM_000033"
     CDS             complement(31301..32095)
                     /locus_tag="ACFIRM_000033"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC3027786.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            32519..33853
                     /gene="gdhA"
                     /locus_tag="ACFIRM_000034"
     CDS             32519..33853
                     /gene="gdhA"
                     /locus_tag="ACFIRM_000034"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC3027787.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            33948..34054
                     /locus_tag="ACFIRM_000035"
                     /pseudo
     CDS             33948..34054
                     /locus_tag="ACFIRM_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34092..34871)
                     /locus_tag="ACFIRM_000036"
     CDS             complement(34092..34871)
                     /locus_tag="ACFIRM_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC3027788.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            35221..35427
                     /locus_tag="ACFIRM_000037"
                     /pseudo
     CDS             35221..35427
                     /locus_tag="ACFIRM_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            35433..35625
                     /locus_tag="ACFIRM_000038"
                     /pseudo
     CDS             35433..35625
                     /locus_tag="ACFIRM_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35826..37229)
                     /gene="mtrE"
                     /locus_tag="ACFIRM_000039"
     CDS             complement(35826..37229)
                     /gene="mtrE"
                     /locus_tag="ACFIRM_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC3027789.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERISYSAEGAALSAQLTRAENLAD
                     LYKVLGGGLKRDTQTGK"
     gene            complement(37282..40485)
                     /gene="mtrD"
                     /locus_tag="ACFIRM_000040"
     CDS             complement(37282..40485)
                     /gene="mtrD"
                     /locus_tag="ACFIRM_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC3027790.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMILVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            complement(40497..41735)
                     /gene="mtrC"
                     /locus_tag="ACFIRM_000041"
     CDS             complement(40497..41735)
                     /gene="mtrC"
                     /locus_tag="ACFIRM_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC3027791.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            41986..42618
                     /gene="mtrR"
                     /locus_tag="ACFIRM_000042"
     CDS             41986..42618
                     /gene="mtrR"
                     /locus_tag="ACFIRM_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC3027792.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAANAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            complement(42711..42923)
                     /locus_tag="ACFIRM_000043"
     CDS             complement(42711..42923)
                     /locus_tag="ACFIRM_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027793.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQDNVHAMIGEIKNAI"
     gene            43255..44823
                     /gene="mtrF"
                     /locus_tag="ACFIRM_000044"
     CDS             43255..44823
                     /gene="mtrF"
                     /locus_tag="ACFIRM_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC3027794.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVMKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            complement(44893..48102)
                     /gene="recC"
                     /locus_tag="ACFIRM_000045"
     CDS             complement(44893..48102)
                     /gene="recC"
                     /locus_tag="ACFIRM_000045"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC3027795.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKSALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEEETPVFEEGGRDTLLHALQTDIQNLKMPSETVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVHWGLDGAMRGGTDQLFTWKQAADRMVLGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLPDRLFEAGADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTSVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQFGETEVLAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(48203..49612)
                     /locus_tag="ACFIRM_000046"
     CDS             complement(48203..49612)
                     /locus_tag="ACFIRM_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC3027796.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            complement(49956..50311)
                     /locus_tag="ACFIRM_000047"
                     /pseudo
     CDS             complement(49956..50311)
                     /locus_tag="ACFIRM_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            complement(50411..51274)
                     /gene="ccoP"
                     /locus_tag="ACFIRM_000048"
                     /pseudo
     CDS             complement(50411..51274)
                     /gene="ccoP"
                     /locus_tag="ACFIRM_000048"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            complement(51298..51468)
                     /locus_tag="ACFIRM_000049"
     CDS             complement(51298..51468)
                     /locus_tag="ACFIRM_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC3027797.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            complement(51473..52084)
                     /gene="ccoO"
                     /locus_tag="ACFIRM_000050"
     CDS             complement(51473..52084)
                     /gene="ccoO"
                     /locus_tag="ACFIRM_000050"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC3027798.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            complement(52111..53544)
                     /gene="ccoN"
                     /locus_tag="ACFIRM_000051"
     CDS             complement(52111..53544)
                     /gene="ccoN"
                     /locus_tag="ACFIRM_000051"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC3027799.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            53870..55618
                     /locus_tag="ACFIRM_000052"
     CDS             53870..55618
                     /locus_tag="ACFIRM_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC3027800.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITH
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            55683..56111
                     /locus_tag="ACFIRM_000053"
     CDS             55683..56111
                     /locus_tag="ACFIRM_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC3027801.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPALAYCRTGTRCY
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(56612..57046)
                     /locus_tag="ACFIRM_000054"
     CDS             complement(56612..57046)
                     /locus_tag="ACFIRM_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC3027802.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(57049..57711)
                     /gene="exbB"
                     /locus_tag="ACFIRM_000055"
     CDS             complement(57049..57711)
                     /gene="exbB"
                     /locus_tag="ACFIRM_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC3027803.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(57777..58628)
                     /locus_tag="ACFIRM_000056"
     CDS             complement(57777..58628)
                     /locus_tag="ACFIRM_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC3027804.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            58884..59522
                     /locus_tag="ACFIRM_000057"
     CDS             58884..59522
                     /locus_tag="ACFIRM_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC3027805.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(59594..60235)
                     /locus_tag="ACFIRM_000058"
     CDS             complement(59594..60235)
                     /locus_tag="ACFIRM_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC3027806.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            60378..62591
                     /locus_tag="ACFIRM_000059"
     CDS             60378..62591
                     /locus_tag="ACFIRM_000059"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3027807.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            complement(62655..>62915)
                     /locus_tag="ACFIRM_000060"
     CDS             complement(62655..>62915)
                     /locus_tag="ACFIRM_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC3027808.1"
                     /translation="LENALIEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIK
                     ASPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK"
BASE COUNT        14745 a        17802 c        15905 g        14463 t
ORIGIN      
        1 aacaaatggg ttaaaccagt atccatacat cagctttttt atcatcctac tttttattca
       61 tccgatcggg cagacagatt tcaaagatga aaagcctatt ctattcacac ccatttgatg
      121 tcatttccac acggacgaac aaataaattc ccataaaaaa acggagcaga tacctgcccc
      181 gttttttaat ttaatccgaa tttagaattt tgcaccggat tggtttgcca tatagtcaac
      241 agccgctttg acttcgtcat cgctcaaacc tgcattgccg cctttggccg gcatcgcgtt
      301 aaagccttca agggcatgtt tgtgcaaggt ttctttgcct tttttgatac gcgatgccca
      361 atcgtctttt ttgcctatgc cgggaatacc gggaatcgaa ccgccgtggc acacctgaca
      421 ggttgcttcg aagacttttt taccgtcaac gccgaccgca ggggctgccg cacccttgtc
      481 ttctgccttc gcttctgccg gagctgcact atcggcagga gcagaagctg ttcctgaagc
      541 ggcattgtcg gcaggcgcag cctcatcagg attcgggaaa gaaccgccgc ttttattcgc
      601 catgtaggta atcgcccgtt tgagttcctg atcggtcagg tctgccgcac cgcctttggc
      661 aggcatggcg ttaaagccgt tcagcgcgtg ttggaacaag gtatcgaagc cttgcgcgat
      721 acgcggcgcc cagtcgccgt tgtgttccag tttcggagcg ttcggcacat tgctgtctgc
      781 cgcgtggcat tggatacaga ttttgccgaa aatctgttcg ccttggcgtt cgccgacggg
      841 gatgccgtca cccatcgtca attgtccgac aggctggatg cgggtctgcg ttgccgcttc
      901 cgtagtggca tcgacatcgc cgaacgatcc actgcccgcc agcttaatca ggaaataaag
      961 gactgcaata acaataacga taccgctcac aagggtaaac agtgcagagc cttgggcttt
     1021 gttgtcgcgg agttgtttca tttggtaggc ctcgccgtca ggttaggttg tgctgtagat
     1081 tatagtttgg cgtgttaaac acagttaaca atattttgct ggattatact gaattcacag
     1141 ggtctttcca atcgctatca ttgaaaatat gaaaaaattt gccaatggta tctgtacaaa
     1201 acaaataatc cgttgaaaat accggattat ctccaagaaa actccccttg tgccgccata
     1261 cgccgcctgc cggcgcaaga taacctttgc caatttgcag aatttacgtt aaccttacgt
     1321 tttccgcacc catagctcag ttggaagagt gtcagtttcc gaagctggag gtcacaggtt
     1381 cgatccctgt tgggtgcgcc aattataaag aggccgtctg aaagataaat atttttcaga
     1441 cggccttttg acttacttca aattcttatt tcaacacttc ggcaagcgca tcggcgacat
     1501 aagtgatatt tgatgtattc agcccggcga cgcacatcct gccggaatcc agcaggtaaa
     1561 cggcaaattc gtcgcgcaac ctgcggactt gccccacgct caaccccgtg tagccgaaca
     1621 ttccgcgctg tttgatgaaa taagtgaaat cgcggtcggg gattcgggcg gtcaatacgc
     1681 cgtaaagttt ctgccgcatc gcacggatgc ggtcgcgcat catataaact tcgttttgcc
     1741 acaaggcgta aagttcggag ctgttcatca cgtcggcggc gatatacgcg ccgtgcgcgg
     1801 gcgggctgga gtagatgcgg cggacggtga atttgagttg tccgaacacc aaatccgctt
     1861 cttctttatt ggggcaaacc acgcttaagc cgccgacgcg ttcgccgtag agcgacaggt
     1921 tttttgaaaa cgaattgctg acaaacagcg gcaaatccat ttccaccgct ttgcggacgg
     1981 cgtaggcatc gctgtccaaa tcgccgccga agccttggta ggcgatgtcc ataaacggaa
     2041 tcagtttgcg cgttttgatg atctgcaaca cttcgtccca ttgccgttcc gacatatcca
     2101 cgccggtcgg gttgtggcag cagggatgga ggatgaggac gctgttttcg ggcagggtgt
     2161 tgaaaaacgc ggtcatttcg tcgaatttca cgccgacagt ggcagggtcg taatatgggt
     2221 aagtgccgac ctcgaaacct gcgccttcaa aaatgccgcg atggttgtcc caagtcggat
     2281 cgctgacgta ggcgcgcgct tcgggaaacc agcggtgcag gaaatccgcc ccgactttga
     2341 gcgcgcccga gccgcccaga gtctgtacgg taacgatgcg tccctgcgcg agcgcggggt
     2401 tgcctttgcc gaacaacaaa tgctgcaccg cgctgcggta aacgtccaaa ccttccatcg
     2461 gcaggtaggg agacggcgca ggcacagcgg cacgggcggt ttcggcacgg cttacggatt
     2521 ccaatacggg cattctgcct tcgtcgtcga aataaatgcc tatgctcaaa ttgacttttt
     2581 cggggcgcgg gtcgtttttg aaggtttcga ccaaactcaa aatcgggtcg ccgggatagt
     2641 attcgatgtg tcggtacata gtccttacct tttgcttttc aaaggatttt ctttttcaac
     2701 agtacgccgc tttcgatatg gtgcgtaaac gggaattggt cgaacagggc ggcacgtccg
     2761 accgtatggg tttccgtcaa ggtgtccaaa ttggcgcgca gcgtttcggg attgcaggaa
     2821 atgtagatga tgttgtcgaa ctgcgacacc agcttcaaag tttcctcatc gatgccggca
     2881 cgcggcggat cgacgaaaat agtggaaaat gcgtaatccg tcaaaacaat accgctctcc
     2941 ttaaggcgtg taaactcacg ttttccggta taggcttcgg taaactcctc agcagacagg
     3001 cgggcgattt tgatgttgcc tatccagttt gcctcaatat tccattgcgc cgcactgacg
     3061 gaggttttgg agatttcggt tgccaaaacc tgtcggaaat atcgggacag cggcagggta
     3121 aaattgccgt tgccgcaata cagttcgagc aggtcgccgc ccaagccttc cgccgcgcgg
     3181 cacgcccatt cgagcatttt ccggcataca gcggcattcg gctgggtaaa gctgccttcg
     3241 atttggcggt agtggaaatc gcggtcgccg acgcgcaggg tttccgtcac atagtcctgt
     3301 tttaagacta ttttctgccc cctgctccgc ccgataacgg aaatatccaa ctgttgctgt
     3361 aacgcttgcg ccgccttcat ccactcatca tcaagccttt tgtggtaaat catggtaacc
     3421 agcatttccc cgctgagcgt ggacagaaat tcgacggcat accaatggtt tctcagttcg
     3481 gcagattggg cggcaacggc aatcaattcg ggcatcaggc agttgactgc ctctgaagca
     3541 gcatcgaaac ggtcgcaacg gatcaggctt gccccgctag ccttctgccc tttttcaaac
     3601 atggcgtaaa acatttcacc gccttcgtgc caaatgcgga attcggcgcg catgcggtag
     3661 tgtttgtccg gagattcgta cacttcccac tcaggaacat ccaaacctgc aaaaagggtt
     3721 ttaaggtagt cttttttacc ttgaagctgc tgcgtgtagt cattcatatc gctatcctga
     3781 aaaatcaggc cggattataa gccgattttc ctcgccgcac gcaaactctt tgactgccgc
     3841 cccttcaatg acggacgggc ttttgtgcta aaatccgcca tctttccaca ctataccgat
     3901 aaagggaaaa atcatggcag gcaacacttt cggacaaatc ttcaccgtga ccaccttcgg
     3961 cgaaagccac ggcgcgggtt tgggctgcat catcgacggc tgcccgcccg gcttggaatt
     4021 aagcgaagcg gatatccaat ttgacctcga ccggcgcaaa cccggcacca gccgccacgt
     4081 tacccaacgc cgcgaagccg accaagtcga aatcctctcc ggcgtattcg aaggcaaaac
     4141 caccggcaca cccatcgccc tcctaatccg caacaccgac cagcgcagca aagactacgg
     4201 caacatcgcc accgccttcc gccccggaca cgccgactat acctattggc acaaatacgg
     4261 tacgcgcgac taccggggcg gcggcaggag ttccgcccgc gaaaccgcag cccgcgttgc
     4321 cgccggagcc gttgccaaaa aatggctgaa agaaaaattc ggcacggaaa tcaccgccta
     4381 cgttacccaa gtcggcgaaa aaaaaatccg gtttgaaggc agcgaacaca tttcccaaaa
     4441 tccttttttt gccgccaacc aaagccaaat cgccgaactg gaacactata tggacggtgt
     4501 gcgcaaatcc ttggattccg tcggcgcgaa actgcatatc gaagccgcca acgtccccgt
     4561 cggtttgggc gaacccgttt tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg
     4621 catcaacgcc gtcaaaggcg tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg
     4681 cagcgaacac ggcgacgaac tgaccccgca aggcttcctg tccaaccatt cgggcggcat
     4741 cctcggcggc atcagcaccg ggcaagacat ctgcgtcaat atcgccatca aacccaccag
     4801 ctccatcgcc acaccgcgcc gaagcatcga cattcacggc aaccccgtcg aactcgccac
     4861 ccgcggcagg cacgatccct gcgtcggctt gcgcaccgcg ccgattgccg aagctatgct
     4921 cgccttggtt ttaatcgacc acgccctgcg ccatcgtgcg caaaatgcgg atgttgccgc
     4981 ggatacgccc gatatttccc gctcggacaa gtaaaaagat ttagccaaaa cacgggcttt
     5041 atactaaaat accgaccatt gccgtgccgt ctgaacgcac gattcttaga ggaatacaac
     5101 cgaaatgaca caagaaaccg ctttgggcgc ggcactgaaa tccgccgtcc aaactatgag
     5161 caaaaagaaa cagaccgaaa tgatcgccga ccacatctac ggcaaatacg atgtattcaa
     5221 acgcttcaaa ccgttggcgc tcggcatcga tcaggatttg attgccgctt tgccgcaata
     5281 cgattccgca ctgattgcac gcgtcctcgc caaccactgc cgccgtccgc gctatctgaa
     5341 agccttggcg cgcggcggca aacgtttcga tttgaacaac cgtttcaaag gcgaagttac
     5401 ccccgaagaa caggcgattg cgcaaaacca tcctttcgtg cagcaggctt tgcaacagca
     5461 gtccgcccaa gctgccgccg aaacgccgtc tgttgaagcc gaagcagccg aatcttccgc
     5521 agcagaataa atccccaaac gaaatgccgt ctgaaaaccg atttggtttc agacggcatt
     5581 ttttcgtatg cggcaatcac ggctcaaata tcgagttgcg ccgtatcgcc ttcgcgttcc
     5641 atccaagcgc ggcgggcggc ggcttcgcct ttgcccatca gtttgacgaa gatgtcgcgc
     5701 gtctcgtcat ccgcgccttc ggggatttgc acctgcaaca ggcggcgggt gtcggggtgc
     5761 atggtggtgt ctttgagctg gtcggggttc atctcgccca agcctttgaa acggctgatg
     5821 gaataggcgg tttctttaac gccttctttt tgcagccgct ctaaaatgcc gtcaagttcg
     5881 ttttggtcga gggcgtagaa tttgcgggcg ggtttgcttt tgccttgtgc gttgacatcg
     5941 acgcggaaca gcggcggctg ggcgacgtag atgtgtccgt cggcgaccag tttggggaag
     6001 tggcggtaga acagggtcaa cagcaaaacc tgaatatgcg aaccgtccac gtcggcatcg
     6061 gacaagatgg ctattttgcc gtagcgcagg ccgcttaagt cgggatggtc gttgatggcg
     6121 tgcggatcga caccgatggc gacggaaatg tcgtggattt cggcgttgcc gaaaagctgg
     6181 tcggggtgga cttcaaagct attgagcact ttgccgcgca ggggcaggat ggcttgtgtg
     6241 gctttgtcgc gggcgagttt ggcggagccg ccggcggaat cgccttcgac gaggaagagt
     6301 tcgttttcgc ggatgtcttc gctttcacag tcggtcagct taccgggcag gatggcgacg
     6361 ccgctgcctt ttttcttttc gattttttta accgaacgta tccgtgcctg tgcctgacgg
     6421 atggcgagtt cggcgatttt tttgccgaag tccacgtttt ggttcagcca caattccaaa
     6481 ggatcgcccg atacggcggc gacaagtttc agcgcgtcgc ggttggtcag cttgtctttg
     6541 gtctgacctt ggaattgcgg gtcgaggacg cgggcggaga ggacgaaggc ggtttttccg
     6601 aacacgtcgt cgctttgcac tttaacgccg cgcggtaaga ggttgtgcag gttgatgaag
     6661 ttgttgacgg cgttgaacac ggcttgtttc aagcctgctt cgtgcgttcc gcccagcgag
     6721 gtcgggataa ggttgacgta gctttcgttg gcgcacgagc cttcttccag ccaagtcaaa
     6781 gcaaacgcgg caccttcgcc aatgctgaaa tcgccgttgt ggccgtctga aatgtagttt
     6841 tcgcaggaga acagcggcac ggcttcctgt gcgtcggcaa tcaggtcggt cagatagctt
     6901 ttcagaccgt cggggtaatg ccaggtttgg gtgtgcgctt cgtcttcgcc tttgaccgga
     6961 cgggtcaggg aaacgcgcac gcccggcagc agcacggctt tggcgcgcaa tagacgctcg
     7021 agttcgggaa tgctgtaatt cgggctttca aaatatttgc cgtccggcca cacgcgcact
     7081 tcggtgccgc tgtctttgac ggcgcatttg cccacttgtg ccaacggttc gaccacatcg
     7141 ccgtcggcaa acacgatgcg gtggactttg ccgtctcgtt tgacactgac ttcaagtcgg
     7201 gtggaaagcg cgttggtaac ggatacgccc acaccgtgca ggccgcctga aaaggcatac
     7261 gcactgccgc cgtctttttt gttgaacttg ccgcccgcgt gcaggcgggt aaacacgagt
     7321 tcgactacgg gtacgccttc ttcagggtgc aggccgacgg gaatgccgcg cccgttgtcg
     7381 cgcacggaaa gcgaaccgtc ttcatgaatt tgcacgtcga tttcagtggc gaaaccgccc
     7441 aacgcctcgt ccgccgcgtt gtcgatgact tcttggcaga tatgtgtcgg gctgtcggtg
     7501 cgggtgtaca tgccgggacg ttctttgacc ggctccaagc ctttgaggac ggtgatgctg
     7561 gattcgctgt attggttgtt tttagccata ggaataatct gaaagtaaga aaaacaacgc
     7621 tttcagacgg cctgaaagcg ttgcgttccg ttattttacc ggttgccgga aggttggcgg
     7681 gcggcaaagt cttcataact ttccataccg cgcaggaagc gggaagagag ttctttcaac
     7741 gcccccaaat aaacgtcgcg tttgaaatca atcacttcgt caacgggcgc ccaatattga
     7801 tgccaacgcc agccgtcaaa ttcggggtgg cgggtggcgc gcaggttgac atcgcaatcc
     7861 cggccggtca ggcgcaggag ataccaaatc tgcttctgcc cgcgataaga gccgcgccat
     7921 tcgcggcgca cccagttgtt cggcacgtcg taacgcagcc agtcgcgcgt ccgcccgacg
     7981 attttgacgt gttgcggcag aagtccgact tcttcgtaaa gttcgcggta catcgcggtt
     8041 tcggggcttt cgccgggctt gatgccgcct tgaggaaact gccatgaatg ttcgcgcacg
     8101 cgcttacccc aaaagacttc gttacgttcg ttaattagga taataccgac attggggcga
     8161 tagccttccc tgtccaacac ggtgtcgccc tccgttaaat tcaatcttgg gattttccca
     8221 caaatcggac ggttttgaca aatcggacgg tatggttcaa gcatgtcgaa acacggacgg
     8281 attcgggaaa atatcttaaa tttggtttac aataatacgt ttcaaattaa ttccggaatc
     8341 agactatgtt agatatccaa ttgctccgca gcaacaccgc cgccgttgcc gaacggcttg
     8401 cacggcgcgg ttatgacttt gataccgcac gttttgacgc actggaagag cgacgcaagt
     8461 ccgttcaggt gaaaaccgaa gaactacaag cctcgcgcaa cagcatttcc aaacaaatcg
     8521 gcgcactgaa aggtcagggc aaacatgaag aggcgcaggc ggccatggat caagtcgccc
     8581 aaatcaaaac cgatttggaa caggctgccg ccgatttgga tgccgttcaa aaagaattgg
     8641 acgcatggtt gttgagcata cctaacctgc cgcacgaaag cgtacctccc ggtaaagacg
     8701 aaaccgaaaa cgtcgaagtc cgcaaagtcg gtacgccgcg cgaatttgat tttgaaatca
     8761 aagatcatgt cgatttgggc gagcctttgg gcttggattt cgaaggcggc gcgaaacttt
     8821 ccggcgcacg atttaccgtg atgcgcggac aaatcgcccg tctgcaccgc gccttggcgc
     8881 aattcatgct cgatacgcac atgctgcaac acggctacac cgagcattac acgccttata
     8941 tcgttgacga tacgacgctg caaggcacag gtcagctgcc caaatttgca gaagatttgt
     9001 tccacgttac ccgcggcggc gatgaaacca aaaccacgca atatctgatt ccgacagccg
     9061 aagttaccct gaccaatacc gttgccggca gcattatccc gtccgaacaa ctgccgctga
     9121 agctgaccgc gcattcgccc tgtttccgca gtgaagcggg atcatacggc aaagacacgc
     9181 gcggcctaat ccgccagcac cagttcgaca aagtagaaat ggtacaaatc gttcatcccg
     9241 aaaaatcata cggcgcgctg gaagaaatgg tcggccatgc cgaaaacatc ctgaaggctt
     9301 tggaactgcc ctaccgcgtg attaccctgt gtaccggcga catgggtttc agcgcggcca
     9361 aaacgtatga cttggaagtt tgggttcccg cacaaaatac ctaccgcgaa atctcaagct
     9421 gctccaactg cgaagatttc caagcacgcc gcatgaaggc gcgtttcaaa gacgaaaacg
     9481 gcaaaaaccg cttggtacat accttaaacg gctccggttt ggcagtcggt cgcactttgg
     9541 tcgcggtttt ggaaaaccat caaaacgccg acggcagcat caatatcccc gccgcactgc
     9601 aaccgtatat gggcggtgtt accaagttgg aagtgaaata agccttaacc ctgaacataa
     9661 atgccgtctg aaacctgttt cagacggcat ttcctttaaa ctcttaaaac acatcaaccg
     9721 tcggcacgaa ccgcattgcc gcaatcgccg gtctgtccga cctcgcggat attggacagc
     9781 gtaacttccg aaatattacc caacgcctct tccgtcaaaa atgcctgatg tccggtaaac
     9841 agtacgttat gacaagacga caggcggcgg aacacgtcgt cggtaatcac atcgttggat
     9901 ttgtcttcaa aaaacagctc gcgctcgttc tcgtacacat ccatgcccaa tgcaccgatt
     9961 ttccggcatt tcaacgcctc gatggcggcg gcgctgtcaa tcagcccgcc ccggctggtg
    10021 ttgataatca tcacgccgtc tttcattttg tcgaacgccg cttcgttcag catatagtgg
    10081 ttttccggcg tggcgggaca atgcagcgtg atgatgtccg atcgggcata cagctcgtcc
    10141 aaatccacat acctgccgcc gagtttttcc acttcggggt tgcaaaacgg atcgtaagcc
    10201 agcaggttca taccgaaacc ctttaaaatc cgcatcgttg cgataccgat tttccccgtg
    10261 ccgataacgc ccgccgtttt gccgtacata ttgaagccgg tcagaccttc cagcgaaaaa
    10321 ttcgcatcgc gggtacgctg gtaggctttg tggatacggc ggttcaaagt cagcatcaga
    10381 ccgaccgtat gttccgcaac cgattcgggc gaataggcag gcacgcgcac gactttcaag
    10441 cccaactctt cagccgcctt caaatccaca ttattgaagc cggcacaacg caacgccaca
    10501 gttttcacgc caatttgcgc caatttttcc aacacgggcc ggctggcgtc gtcgtttaca
    10561 aaaatacagg ccgcttccgc gccttccgcc attttcgccg ttttcgcatc cagcatgaaa
    10621 tcaaaaaact ccagctcgaa gccgaaatgc cggttggcgc gggtaaaatg ttcgcggtca
    10681 tagcttttcg taccgtaaat cgcaatcttc atcaatatgt ccagttgtcg tctatggttg
    10741 agaaacggca taatacaccg aattcaaaca aatcagtaga atatggcgga ttaaaattga
    10801 tttcatgcac ggcatttcca tttcaaaaca caaaactcaa tcgcccatcg ccgccagaag
    10861 ctccgcctga tgttcggcaa tcagggcatc ggtgatttct gccaaatcgc cgtccatcac
    10921 aaaatccagc ttgtgcaggg tgaggttgat gcggtggtct gttaccctgc cttggggata
    10981 gttgtaggtg cggatgcgtt cgctgcggtc gccgctgccg atgagggatt tgcgttcggc
    11041 ggcttctttg gcttggactt cgcgtttttg cgcgtcgttc aggcgggcgg cgaggacttt
    11101 catcgcctgc gctttgttgg cgtgttggct gcggccgtct tggcattcga ccaccatgcc
    11161 tgtcggcaag tgggtaatgc ggacggcgga gtcggttttg ttaatgtgct gaccgcccgc
    11221 gccggatgcg cggaaggtat cggtgcgcag gtcggcgggg ttcagttcga tgtcttcgag
    11281 ctcgtccgct tcgggcatga cggcaacggt acaggcggag gtgtggatgc ggccttggct
    11341 ttcggtggcg gggacgcgct gcacgcggtg tccgcccgat tcaaatttca gacggctgta
    11401 cgcgccgagt ccgacaatac gggcgatgac ttctttatag ccgcccaatt cgctttcgtt
    11461 ggcggacacg atttcaacct gccagcggtt gcgttcggcg tagcggctgt acatacgcag
    11521 caaatcgccg gcaaacagcg cggcttcgtc gccgcccgtg ccggcgcgta tttcgataaa
    11581 gatgtttttg tcgtcgtcgg catctttggg cagcagcagt ttttgcaatt cggtatcgag
    11641 ttcgtcgatt ttggctttcg ccgcttcgat ttcttcggcg gcaaagtctt tcatttcggg
    11701 gtcggacaac atttcttcgg catccgccaa gtcgctttgg gcaagccgat agttttgaaa
    11761 tacttcgacg acgggggtca gttcggcgtg ttcgcgcgtg agcttgcggt agttgtccat
    11821 atcggatgtg gcttcgggtt gccctaaaag gtgggtaact tcttccaacc ggtcgccgag
    11881 ttgttgcagt ttttctaaga tagacggctt cataattctt ccataacaaa cgccgcctga
    11941 atttcagacg gcatcaaaac gcggtcatta taatatattt tctgaatatt caaaaagata
    12001 atctagggac ggagcagact gtcggcattg cgttccgtta tcgcggcgat ttccgccgcg
    12061 ccggtaccgc gtatttccgc agcaatttcg gcaatccgaa gaatgttggc aggcgtattg
    12121 acggtgtttt ccaacatgaa cgggctatcc gtttccaata cgaaatcccc gtcgttcaag
    12181 gctttaagcg tatcgcgcac tttacgcgcg ttcggattga gcagcagcga accgatgccg
    12241 attttgaacc ccagttttgt caacacgcgc gcttcttccg cgctgcctga gaaagcgtga
    12301 acgatgccgc cttgagtaaa gcctgtctgt ttgacggcgg cggcgatgtc ggcggtggct
    12361 ttgagattat ggataatcac gcggcggcgc agggtttgcg cgatggcaag ctggcggcta
    12421 aaaacttgaa tttgccgttc acgctgcggc ggcgtttggg ttttgtcgta aaaatccaag
    12481 ccgatttcgc cgacccacgc tcgggggtaa cgcaccaata cggcttccaa accggaaaaa
    12541 tcctgttccg caatgccgtc tgaaaaccaa ggatggatgc ccagcgcaat acggatttga
    12601 ccgtgttcgg acggcatttc cgacagttcc gccacgtcct gccaatcctg cggacaggtg
    12661 gcgggaacga taaaccgcca cacgcccgcc tgtcgcgctt cggcaagcac ctgcgccaga
    12721 ttgccgcgca aaacggaatc ggcaagatgg caatgtgtgt cggtcagcct catcttcaca
    12781 ccagccccaa ccgctgcaac tcgtgtttca ccccgtccgc gccccatcct tcggaaacgg
    12841 caagcgtcat caatgcggtt gcggtttcca aattgcattt gccgccgttg accacgcccg
    12901 cccgacgcag cgcgtcgccc tgtgcgtaaa cggcggcagc gcaaccctgc ggcacttggc
    12961 tgatgttaag caacaatttg ccttgctgcg taaagtcttg cacggcgcgg atgaagcctt
    13021 cgccggcggg ggtattgccg tgtccgtagc tttgcaggat aagggcttgg gcgggaagct
    13081 gtccgagccc gtcggcaagt tcttggacgg caaagccggg gataagcgtg cggacggcga
    13141 tttttgcctg cgggtcggga taacggattt cgaggccgtc tgaaacatct gccgcgtcct
    13201 gattcgggat gcggacattg tgccagcccc gggtttcgtc ccactcggca agtgcgccga
    13261 aatgggcatt gccgaaaccg gcggcggttt ccgtgctgat tttgctgctg ccgacggcgg
    13321 gatacagttt gccgtcaaag gcaataagcg tctgcttcaa tccgagtggg aatgcggcaa
    13381 cggcagtcac cagattgcgc ggcgcgtcgc tgccctctgc gttgtacggc cattgcgaac
    13441 cggtcaggac gatcggtttg tccaaacctt gcagggcgag ggcgaacata ttggcggtat
    13501 aggcgagtgt gtccgtgccg tgcagcacca aaacaccgtc gtactgcggc agcgcggcgg
    13561 caatgatgtc cagccaagcg caccagtttt gcagttggac ggcggaagaa tcaatcagcg
    13621 gctcacaaac gtgccaatcg aaatccatgc cgtctgaaaa cggcgcgagt gcctgcccga
    13681 ccagcgcggt atcggggcgc aagcccgcac tgctttgcgt catgccgatg gtgccgccgg
    13741 tataaaggac aaaaattttc tgtttcatgg ctcattctga aaatgcaaag accgcctgaa
    13801 acgcgaaatg cgtccggacg gtcgaaaatc cgatttactg ctgcttctgt gccgcttttt
    13861 tcgctgcctt ttccgccttg cgtttggcgc gtttttcgct caattgtgcg cggtaaagct
    13921 gataatgtcg gcgtttgcgc caccagaacc acgccagcgc cgccgccagc acgcccaatg
    13981 cgatgaagat gcccgattgc aggctgtgca ttttcgccat cagccaatcg atgttgtgcg
    14041 cgccgtactc gcccaagtaa atccaaacgg gcacggaaat cagcgcggca agcccgtcca
    14101 taatcagaaa gcgcagatac gatactttgc ggctgatgcc ggcggtaacg aaaacggcag
    14161 tccgcaaacc cggcaggaaa cgggcgacaa acagaaccca gttgccgtat ttgtcgaatt
    14221 tttcctgaac ctgcgcgtaa cgtttcggcg tcatgatgcg cgcaatcggt ttgaacttga
    14281 ggattttctg cccccagatg cgtccggcgg caaacatcac gccgtcgccc gccaacacgc
    14341 cgagcatacc gaccgcaaac ataatgtgcg gattggtata acccataccc gaaatcacgc
    14401 cgcccgttac caaggtcaaa tcttcgggaa tcggcacgcc gaaaccgcag atgaccaaaa
    14461 cgaaaaacac ggccgcatag ccgtattcga caaaaaaggc ttccaaaagg gcaaacatag
    14521 cggatattcc attgtcggag ataaaaagtc agaacaagcc gaaacatttt ctgcgcgaaa
    14581 cggcattcta tcaaagatta tgccgtctga aagcggcagg gggggcagat tcgcgccccg
    14641 ccggacgttc agacggcatt taaacaggaa aaccgccgct gttgaaacgg ttttgcctga
    14701 ttttgcctaa atgccgccga tggcggcggc gatacgctcc gccccgtcgc gcgcccaatc
    14761 ggcctgacgc gcctcgacca tcacgcgcac gacgggttcg gttcccgaag cgcgcaacac
    14821 gacacgccct ttgccttcga gttctttttc cacttcggcc aacacgtctt tcgaagcctc
    14881 ctgccatttc tgaccctttt ggatgcgcac gttaatcatc gtttgcggat acggctgcca
    14941 gtcggcgcaa acggtggcga ggtcttggtt caggatttgc agcgccgcca agacttgcag
    15001 cgcggaaata atgccgtcgc cggtgttatg tttgtccatg cacaaaatat ggccgctggc
    15061 ttcgccgccg atgagccagc cgcgttggtt cagctgttcc aacacatagc ggtcgccgac
    15121 tttggcgcgg cagaaatcga cgccctgctc tttcagggcg atttccatcg ccatattggt
    15181 catgaccgtt ccgaccacgc cgccgatgtt gataccttct cgggcgcggg ctttggcgat
    15241 gacgtaaatc aggctgtcgc cgtcgtaaac ctgcctgttt ttatcgacca tcatcaggcg
    15301 gtcgccatcg ccgtccaagg caataccgta atcggcttcg ttttgcaaca cggcggcttg
    15361 gagggttttg gtatgggtcg cgccgcattt ttcgttgatg ttgtagccgt tgggttcgtt
    15421 gccgatgctg acgacctgtg cgccgagttc gtggaacact ttgggtgcaa cgccgtatcc
    15481 tgcaccgttg gcggtatcga taaccaactt caaaccccga aggtcggaat ggccggggaa
    15541 ggtggatttg caaaactcga tatagcggtc gtccgcaccg ctgatgcggc gggcgcgtcc
    15601 gagacgtgcg gacggttggg ttttcatttc ttcatcgatt ttggcttcga tttccaactc
    15661 gacttcatcg gaaagtttca cgccgccttc ggcaaagaat ttgatgccgt tgtcggaata
    15721 ggtgttgtgc gacgcggaaa tcatcacgcc ggcggacagg cgcaatgcgc gggtcagata
    15781 agccacaccg ggcgtgggca acggcccggt ctgtaccaca ttcacacccg ccgccgtaaa
    15841 gcccgctacc agcgcggctt ccagcatata gcctgaaatg cgcgtgtctt tgccgatgag
    15901 gacggtcggt ttctgatcgg tatcgtgctg caccagcacc tgccccgccg catagccgag
    15961 tttcaataca aaatcgggcg taatcggaaa ctgaccgact tcgccgcgca cgccgtccgt
    16021 gccgaaatat ttttttgcca tcatttgctc cgtgaatgtg aaccgttgtc cgagattata
    16081 cagtcagttt gcgccctgct gcctgcaccg ttgatgccgt ctgaagccgt cccgcccttt
    16141 tcagacggca ttcggcgcaa aaaccgccgt ttaccggttc acgcccaacg cttcccacac
    16201 cttcaacgcg tccgccgtcg ccttcacatc gtgcacgcgc acgatttgcg ctccgcgtgc
    16261 cacggaagcc aaagccgctg ccacgctgcc gtgtacgcgc gccgccgcgt ccgcctcacc
    16321 ggtcagctca cctatcatgc gtttgcgcga cacaccgatc agcagcggca aaccggtttc
    16381 cgccatcaat tcgggcaaat gccgcatcag tacgatattg tgttgcaggt tcttgccgaa
    16441 gccgaaaccc gggtcgagcg taatccgttg cggcgcgatg cctgccgcga cacaggtttc
    16501 tgaccgtgtt ttcagataac gtgccacttc gccgaccaca tcctgatatt tcggattgtc
    16561 ctgcatggtt tcgggcaagc cccgcatatg catcaggcaa atgcccgtgt ccgcctggcg
    16621 cgccagcaat tcgaccgcac cttcgtcggt caacgccgcc acatcattga taatatcgat
    16681 gccgccgagt gccaacgcct tttccataac caccgtgcgg cgcgtgtcca aactgacggg
    16741 aacgccccac cccgccgctt ccgccaatac aggctcaacc cgcgcccatt cttcttcggg
    16801 cggaacgaaa tccgcacccg gccgcgtcga ttcgccgccg atgtcgagaa tgtccgcacc
    16861 ctctttcaaa agccgttcgg catgcgccaa agctgtttgg gcgttttgcg aatacgcgcc
    16921 gccgtcggaa aaagaatcgg gcgtgagatt cacgatgccc atgattttcg gtttgtccaa
    16981 accgatttca aaccgtcctg cccgccaaac gtgtcgtgcc atctgaactc ctcccgaaat
    17041 aaaaaacaga ttatatgccg tctgaaaccg tcttgtgcgc ttcggacggc accgctattc
    17101 gggcggcaga cggcatgttg tcccaatgtc tgcgccgcct ttgaatctgc cggcatgcct
    17161 gctatccgcc cgccttttca aaacaggttc cgacgattcc gcacgcgcct gccgcctttg
    17221 ccaagccgta caggatttcc tgcggcatat cgcggctcca taatcccgta atattcgcaa
    17281 tcacgggcag atggctgatt tggcggactt tcacgatgga ttcgacatcc aaacggtagg
    17341 gatggccttt ggtatggccc aacacgcccg actcgcccag aatcaggtgg cggttgccgc
    17401 gcgagacgac atattctgcg gcattcaacc aatcttcggc agaatggtgc ttgtctttac
    17461 acaaaaccaa gggaatgttc aggcttccgg cttcattcaa tacggcaaga tcggacatca
    17521 gcccgccgcc caaatacagg atgtccgctc ccgcattcaa agccgcttcg acatggcgga
    17581 cgttgcgaac gcgcaccaat acgggtttcc ctgcgccatg cgccgatgcg gcctgttccg
    17641 ccaaccgtct gcaccgtccc cgcccttcat ccgcacttaa agtgtcgtat aagtttgccg
    17701 aagtgaaaaa cggatccaga aacactgcat ccgcattgct ccatactgac ggttctgcag
    17761 cgatacggac ggtttccccg ccgccgaaag ccacgccttt ggcggcaacg cggctgtctt
    17821 ccgcccgatt ttcccgactg acggttttcc atgtgtccaa aatgcggacg gctttctcga
    17881 cctccggcag cgtctgtacc tccctgacgc tcaaaaccct atcgtcgccg attgcgccga
    17941 tgacggtacg ctcgtcgccg tgagaaatgt gttctcgcag acctctgctg cggatgaagg
    18001 cgacaacgcc ggcaatgtcc gcttcggcgg cacgcctgct catgacaata atcatatttc
    18061 ctcctgacac aagagacggc ccgcccaaaa taggattttt gcaagccgtg ttatactgtg
    18121 gcgtgtttta cagattgttc gggctatgga tttattatcg gttttccaca aataccgtct
    18181 gaaatatgcg gtggcggtgc tgacgatgct gcttttggcg gcagtcgggc tgcacgcttc
    18241 cgtatatcgc accttcacgc ccgaaaacat ccgcagccgc ctccaacaaa gcattgccca
    18301 tactcaccgg aaaatctcgt ttgatgcgga tatacggcgc aggcttctgc cccgcccgac
    18361 cgtcatcctg aaaaacctga ccattaccga acccgacggc ggccgggtcg ccgtttccgt
    18421 caaagaaacc aaaatcggat tgagctggaa aaacctgtgg tcggatcgga tacaggttga
    18481 aaaatgggtg gtttcgggtg cggatcttgc cctgacgcgc gacagaaacg gcgcttggaa
    18541 catccaagac ctgttcgacg gcgcgaaaca ctccgcctca gtcaaccgca ttatcgtcga
    18601 aaacagcacc gtccgcctca atttcctgca gcaacagctt atcctgaagg aaatcagcct
    18661 caacctgcaa tcccccgatt cgtcggggca gcagtttgaa agttcgggca tactggtttg
    18721 gagaaagctg tccgtcccgt ggaaaagcag ggggctgttc ctttcagacg gcatcggcac
    18781 gcccgaaatc tcaccgttcc attttgaagc ttccacttcg ctggacggac acggcatcac
    18841 catttccacc accggcagcc cttctgtccg cttcaacgcc ggcggagcgg atgccgccgg
    18901 cctcggcctg cgtgcagaca cttccttccg caacctccac ctgaccgcgc aaatccccgc
    18961 actggcactc aaaaacaaca gcatcaaaac cggcacggtc aacggcacgt ttaccgccgg
    19021 cggcgaatat gcccgatggg acggttcgtt caaactcgac aaagccaacc tgcactccgg
    19081 catcgccaac atcggcaacg ccgaaatctc cggcagcttc aaaacaccgc gccttcaaac
    19141 caatttctcc ctcggctcgc cgttggtttg gagtcgggac aacgggctgg acgccccgcg
    19201 cctgcacata tcgacccttc aggataccgt cgaccgcctg ccgcaacccc gtttcatcag
    19261 ccggctcgac ggttcgctgt ccataccgaa tctgcaaaat tggaatgccg aattaaacgg
    19321 cacattcgac cgccaacccg ttgccgcaaa attcaaatat acgcgggaag gcgcaccgca
    19381 cctggaagcc gccgccgcgc tgcaaaaatt aaacctcgcc ccctatcttg acgaatttcg
    19441 gcaacaaaac ggcaaaatat tccccgacat cctcggcagg ctgtccggca acgtcgaggc
    19501 acacctcaaa atcggcagca tccaactccc cggcttgcaa ctggacgata tggaaaccta
    19561 cctccacgcc gacaaagacc atatcgcgct cagccgtttc aagtcagggc tttacggcgg
    19621 ccataccgaa ggcggcatca gcatcgccaa cacccgtccc gccacttacc gcctgcaaca
    19681 gaatgcaagc aacatccaaa tccaaccgct gctgcaagac ctgttcggct tccacagctt
    19741 cagcggcaac ggcgatgcgg tcatcgacct gaccgcaagc ggcgaaaacc gcaaacagct
    19801 tatccgctcg ctgcaaggca gcctgtcgct gaatatttcc aacggcgcgt ggcacggcat
    19861 cgatatggac agcattttaa aaaacggcct ttccggaaaa atctcgggca gcacaccctt
    19921 ctaccgattc acgctcaaca gcgaaatttc agacggcatc agccgccaca tcgataccga
    19981 actcttctcc gacagcctct atgttaccag caacggctat accaatctgg atacgcagga
    20041 attgtctgaa gatgtcctta tccgcaacgc cgtccatccg aaaaacaaac cgattcccct
    20101 gaaaatcacc ggtacggtgg acaagccgtc cattaccgtc gattacggca ggctgaccgg
    20161 cggcatcaat tcgcgcaaag agaaacagaa aatcctcgaa gacaccctgc tggaacaatg
    20221 gcagtggctc aaacctaaag aaccgtaaat accctgcgta caaaaatgcc gtctgaaaca
    20281 cattaatctt cagacggcat gaccgtaaaa cctacaaccc caatttcccc caaatcccat
    20341 caattttaac cgtaaccgca gggtcttttt tgattacccg tccccattcg cggtcggttt
    20401 ctcccggcca tttgctggtc gcatccaaac ccattttgcc gccgagtccg ctgacggggc
    20461 tggcgaagtc gaggtagtcg atgggcgtgt tttccaccaa aacggtgtcg cggacggggt
    20521 ccatgcgcgt ggttaccgcc cagatgactt ctttccagtc gcgcacgttt acatcgtcat
    20581 ccaccacgat gatgaatttg gtgtacataa actggcgcag gaacgaccag cagcccgtca
    20641 tcacgcgctt ggcgtgtccg gcgtactgtt ttttcatgct gaccaccgcc atgcggtagg
    20701 aacagccttc gggcggcagg tagaaatcgg tgatttcgga gaactgcttt tgcaaaagcg
    20761 gtacgaacac ttcgttcaac gccacgccca aaacggcagg ttcgtcgggc ggtttgcctg
    20821 tgtaggtaga gtggtaaatc gggttttcgc gcatggtgat gcgttcgacc gtaaacacag
    20881 ggaaatggcc ctgctcgttg taatagcccg tgtggtcgcc gtatgggcct tccaacgcgg
    20941 tttcgtttgg atgaatcacg ccttccaata caatttcagc acgggcaggc acttgcaaat
    21001 cgctgccgat acatttcacc agctccgtcc gcgaaccgcg cagcagtccg gcaaactggt
    21061 attcgctcaa agtatcggga acgggcgtta ccgcgcccaa aatggtggag gggtcgcaac
    21121 cgagtacgac ggcgacggga tacggcgtat cgggattgag tttgcggaat tcctgataat
    21181 ccaacgcgcc gccgcgatgc gacagccagc gcataaccag cttgtttttg ccgatcagtt
    21241 gttggcggta aatgccgaga ttttggcgtt ttttgtgcgg cccgcgcgtg acggtcaagc
    21301 cccacgttac cagcggcgca acgtcttccg gccagcaatg ttgaatcgga agctgataca
    21361 aatcaacgtc ttctccttcc cacacgattt cctgacacgg cgcgtttttc accacgttcg
    21421 gcgccatgct ccaaatatct ttcaacagcg gcagtttgga aaacgcgtct ttgatgcctt
    21481 tgggcggttc gggttctttc aaatacgcca gcgtctgccc gatttcgcgc agcttggaca
    21541 cgctgtccgc gcccatgccc atcgccacac gttcgggggt gccgaacagg ttcgccaaca
    21601 cgggataatc atagcgcgta ccgtcgggct taatcgggtt ttcaaacaac aacgccggcc
    21661 cttcggcgcg caacacgcgg tcggcaattt cggtcatttc caaatgcggg gaaacggggt
    21721 gggcgacgcg cttgagcttg ccctgctgct cgagcatagc gatgaagtcg cgcaggtctt
    21781 tgtatttcat gtttatcctt tttgtccttt tatcctgaac aatccgattc ggataccgcc
    21841 cctatccttg cctgcgcttc ggcatatccc atgccgtgat aaaagtcgcg taccggcgga
    21901 tgtccgccgc tttgatgaag ttgcagcaaa gggcgttggt tgtcggactg ggtaacgaca
    21961 ttgcagtgca gaccgaaggt gtcggtttca taagggggca gccgattgca aatcataccg
    22021 aaataaacgg cgttttcagg gttgtcgtaa aagcggcttt gatagtcgtt aaaactcttt
    22081 tcgctgacgg acacccacac gccgtattcc agcgtttcct gatgcccgat aatcggaata
    22141 ggcagcgccg cgcggataaa gcggtcggtt tggtcggaat agcggatgat gcagaaatca
    22201 gaatcgcatt ccgcttgata agcaatgcgt tcttcttcac tgagttgatt atagggatag
    22261 ggggcggtaa agccggttgc gggcatatct tcatgttttt cgccgcagga agtgcaagtg
    22321 tgcataaggt ttcagacttt caacacaagt ttgcggtaaa gccattcgcc ggcaaacagc
    22381 atccccatca atacataggc aatcacgcct gtataaaccg cccaccaatc atatcgcccc
    22441 aaccgggcca acaaagcggc aagcgtcccg ttggcaataa agaatacgca ccaaacctgc
    22501 gttacccggc gggtatagcg cacggctttt tcaggcaggt cgggctgttg cagccgcgca
    22561 agtttttcaa tcaccgtctg cccggcaaac aggctgctgc cgaacaccgc caacatcatc
    22621 aaattaacga ggacaggata ccaatacatt gaatcacgcc gcccgaacac caactactgc
    22681 ggcaaaaaac agtgtaatat agtggattaa atttaaatca ggacaaggcg gcgaagccgc
    22741 agacagtaca aatagtacgg caaggcgagg caacgccgta ccggtttaaa tttaatccac
    22801 tataccacaa agcgggatag gcaatgctta atacggtcaa aaaaatatgt ccgaaaaaac
    22861 cgggtttcat tttgaatccg cacaaacgtt ttcagacggc atccgataaa aacatgccgt
    22921 ctgaaaaata attagaaata cccgattagc cggcctgaat cttcaatacc gcctgtacca
    22981 cgtcgttgac ggtgcggaca ttgcggaaat cttcagcctg cagcttgcgg gctgccacaa
    23041 ccattcacga ttgcgatata cccggcggca ttcccgactg ccgttcagca gaaaacgcac
    23101 ccattccaaa ccgctccaat atgcctcggg cagcataccg gcttcagacg gcatatcgtc
    23161 cgaagcagac aaagtcaggt gtaacgcgtc cctttggtca gaaccatcgc caaagcataa
    23221 gcaaacggcg cgcgcgttgc cgatacggca tatccttccg gcagcggatc gtccgccgcc
    23281 aaaaccaaaa ccgacccgca tccctcttcc aacagggacg ccgcttccgc caatgccgtt
    23341 tccacaccgt cggcacacac ggccaatgcc gtctgctcgc tcatatcccg cctcaatatc
    23401 gaccattgcc ccgccgtcgc attgtgcacc gacaaaccga acgaagtcgg cgacacggta
    23461 tgcgatttca acagttccaa ccacaaatcg aaactacgcg ccatttcccc gtcgtgcgag
    23521 gcataaacta ccggactgcc gggatgggcg gaggcaatgt cccaagccgc gtcgcatacc
    23581 aaacgcgccg ccttactcaa acggcggcgc cgcatagcgg gcaggaacgg caattcccgc
    23641 ctgacatcgg gcaaaccgtc ggcaaaatcc ggacattccg cccactgcct ccactgggcc
    23701 atatcgcgca ttttgccgcc cgaaacccgc cagtcggcga tgtcgaagtg gaaccggcaa
    23761 ataatcggcg gcatagtttc tttcaaaaat ttacactgtg ccgcattcta accaaagccc
    23821 atccccctga caatgccgaa attcaaacgc atccacgcca ttttttccga caacgccgcc
    23881 cctcggcaaa ccgcccgaat tagcctgaat ttacatttat cattgataat gcccgattac
    23941 cgacaccgcc tccctgctgc cgcacagcgg gcgtatggtt ctgatagacc gcattacccg
    24001 atacggcgat gattttgtcg aagcaggggc gcaggtaaaa cccgaccaca tcctgctggc
    24061 cggcggcata ctgccctata cggcatttat cgaactgatg gcgtaggctg tcggcgcata
    24121 tgccggcctc caagcccgaa aaaggcgcaa ccggtccggc tcggttttcc tgcccggcac
    24181 gcgcaaactt gaaattttta cccaatccgt ccctgtcgga acacatttgc cggcaacggc
    24241 gcatatgtct atccaagatg ccggggtatg ggcgtgtccg actgcgaact gcgttggacg
    24301 gacgcgccgg aaaccttgtc tgaaacactc ctttcagacg gcattttggc gcgcgcctca
    24361 ctcaacgtgt acagccccga acaccctgtc gaaacaactt aatgccgtct gaacagacac
    24421 gcacatacgg agaacaacca tgactgaaac cgtcctgatt accggctcaa acagaggcat
    24481 aggcaaagcc gccgcactcg gttttggcgg acgacggctt tgatatcgcc gtccactgcc
    24541 gcagccgccg cgacgaagcc gaagccgtgg cggaagaaat ccgcgctttg ggcagaaatg
    24601 cgcgcgtgtt gcagtttgac gtgtccgacc gcgaagcctg ccgcgagatt ctgaccgccg
    24661 acatcgaagc aaacggcgcg tattacggcg tggtgttgaa cgccggcctg acgcgcgaca
    24721 atgccttccc cgcgtttcag acgacgattg ggatgtggtg ccgcggacta atttggacgg
    24781 tttttacaat gtactgcatc cgctggttat gccgatgata cgccgccgca aagccggacg
    24841 gattgtgtgt atggcatcgg tgtccggcct gacgggcaac cgggggcagg tcaattacag
    24901 cgcgtcaaaa gcgggcatta tcggcgcggc aaaagccttg gcggtcgaac cggcgaaacg
    24961 caaaatcacc gtcaactgcg tggcgccggg tttgatcgat acggagattg tcgatgaaaa
    25021 cgtacccgtc ggagaaattt tgaaagccgt ccccgccgcg cgtatgggac tgccggaaga
    25081 agtggcgcac gcagtgcact tcctgatgga tgaaaaagcg gcgtatatca cgcgccaggt
    25141 gattgcggtg aacggaggtt tgtgttgaat accagaaggg tcgcagtaac gggcataggc
    25201 ggcattaccg ccttcggccg ggattggcaa agcatacagg cagcattcaa agccgaaaaa
    25261 aacgccgtca aatatatgga ttggcgcgaa cgtttccccg aattggaagc gcaactgggt
    25321 gcgccgattg aggattacgc gccgccgaaa cattggacgc gcaagcggct cagaagtatg
    25381 gggcgcgtgt cgtacctgtg cgtcgatgcg gcggatcagg cgctggcgga tgccggtttg
    25441 ctcggagacg aaagcattac cgacggacgg atgggcgttg cctgcggctc ttccggcggc
    25501 ggcaccaaag acatcggaga tgtgggagaa ttgttgctga ccggcacgtc gcgcaacttc
    25561 ggcgccaata cctatgtgcg tatgatgccg cacaccgccg acgccaatat cggcatcttt
    25621 ttcggattaa caaggacgca tcatcccgac atcgagcgcg tgttcttcgg gcagccaagg
    25681 cataggttat gcttacgagg ccatcaaata cggtctgacc gatatgatgc tggcgggcgg
    25741 cggcgaagaa ttttgcccgt ccgaagtgta tgttttcgac tcgctctatg ccgccagccg
    25801 ccgcaacggc gaaccggaag agaccccgcg tccatacgac gcgaaccgcg acgggctggt
    25861 catcggagaa aaggcgcggg gattttcgta ttggaagaat tggaacacgc caaacggcgc
    25921 ggtgcgaaaa tttacgccga actcgtcggc tacggcgcca acagcgacgg catcaatatc
    25981 ggcgaaggcg cagccgtatt catcatgacg cgcgatgcgg atttttccgg cggtatgcag
    26041 cttctgggtt acggcgcaag cagcgatgcc taccatattt ccacgccccg ccccgacgca
    26101 aaggcgcaat cctcgccttt cagacggcat tgcagcacgc cggccttgcg cccgaagaca
    26161 tcggccggat taatctgcac ggcaccggga cgcaccacaa cgacagtatg gaaagccgcg
    26221 ccgttgcagc ggttttcggc aacaatacgc cctgcacgtc cgccaagccg caaaccggac
    26281 acacgctggg cgcggcgggc gcaatcgaag ccgcgttcgc gtggggcatt gccgaccggc
    26341 aaagcaatcc cgaagggaaa cttccgcccc ggctttggga cgggcagaac gaccccgacc
    26401 tgcccgccat taacctgacc ggcggcggct gggaaaccga aaaacgcatt gccgccagct
    26461 cgtcgtttgc cttcggggga agcaactgcg tcttaatcat cggatgaaat aagtttgtca
    26521 atcccaccgc tatgctatac aatacgcgcc tactcttgac gggtctgtag ctcaggggtt
    26581 agagcagggg actcataatc ccttggtcgt gggttcgaac cccaccggac ccaccaattc
    26641 ccaagcccgg acgtatgttt gggctttttt gccgccctgt gaaaccaaaa tgctttgaga
    26701 aaccttgata atgaaaaaag tcagcgtatt gattgttgcc aaaaacgaag caaaccacat
    26761 tcgggaatgt attgaaagtt gccgtttcga taaagaagtt atcgttatcg acgaccatag
    26821 tgccgacaac actgccgaaa ttgccgaggg tttgggtgca aaagtcttca gacggcattt
    26881 gaacggggat ttcggagcgc aaaaaacatt tgccatcgaa caggcaggcg gagaatgggt
    26941 tttcctgatt gatgcagacg aacgctgcac gccggaacta tctgatgaaa tctcaaaaat
    27001 tgtccgaacc ggcgattatg ccgcctattt tgtcgaacgc cgcaaccttt tccccaacca
    27061 tcccgccaca cacggcgcga tgcgtcccga cagcgtatgc cgtctgatgc cgaaaaaagg
    27121 cggttcggtg caaggcaaag tacacgaaac cgtacagacc ccctaccccg aacgccgtct
    27181 gaaacatttt atgtaccatt acacgtacga caactgggaa caatatttca acaagttcaa
    27241 caaatatact tccatctcgg ccgagaaata ccgggagcag ggaaagcccg tgagtttcgt
    27301 tagggacatt atcctccgtc cgatttgggg ttttttcaaa atttatatcc tgaacaaagg
    27361 gtttcttgat ggaaaaatgg gttggattat gtccgtcaac cacagctatt acacgatgat
    27421 taaatatgtc aaactatatt atctgtacaa atccggcgga aaattttaaa tggaaaaaga
    27481 attcaggata ttaaatatcg tatcggccaa gatttggggt gggggcgaac aatatgtcta
    27541 tgatgtttca aaagcattgg gacttcgggg ctgcaccatg tttaccgccg tcaataaaaa
    27601 tgatgaattg atgcgcagac gcttttccga agtttcttcc gttttcacaa cgcgccttca
    27661 cacgttcaac gggctgtttt cgctctacgc gcttacccgc tttatccggg aaaaccacat
    27721 ttcccacctg atgatacaca ccggcaaaat tgccgcctta tccgtacttt tgaaaaaact
    27781 gaccggggtg cgcctgatat ttgtcaaaca taatgtcgtc gccaacaaaa ccgattttta
    27841 tcaccgcctg atacagaaaa acaccgaccg ctttatttgc gtttcccgtc tggtttacga
    27901 tgtgcaaacc gccgacaatc cctttaaaga aaaataccgg attgttcata acggtatcga
    27961 taccggccgg ttccctccct ctcaagaaaa acccgacagc cgttttttta ccgtcgccta
    28021 cgccggcagg atcagtccgg aaaaaggatt ggaaaacctg attgaagcct gtgtgatact
    28081 gcatcggaaa tatcctcaaa tcaggctcaa attggcaggg cacggacatc cggattatat
    28141 gtgccgcctg aagcggggcg tatctgcttc gggagcagaa ccatttgttt cttttgaagg
    28201 gtttaccgaa aaacttgctt cgttttaccg ccaaagcgat gtcgtggttt tgcccagcct
    28261 cgtcccggag gcattcggtt tgtcattatg cgaggcgatg tactgccgaa cggcggtgat
    28321 ttccaatact ttgggtgcgc aaaaggaaat tatcgaacat catcaatcgg ggattctgct
    28381 ggacaggctt acgcctgaat ctttggcgga cgaaatcgaa cgcctcgtct taaaccccga
    28441 agcaaaaaac gcactggcaa cggcaggaca tcaatgcgtc gccgcccgtt tcaccatcaa
    28501 ccataccgcc gacaaattat tggatgcaat ataagccgct ttcagacggc ctatgccgtc
    28561 tgaaagcctt tgatgcaaca aaccgccaaa ttatattcgt tcattggaaa gaaacacccc
    28621 gaattcatcc ttcaaaataa gaaaatccca atatcccccg atatcgcgca gcctattggc
    28681 aaagttttgc agcgtcttcc ccggcttgtg ccgccgcgtc aagtgctttg ttacaatgca
    28741 ctaccttcac atttcttaat aaattttatg agtaaccata cttcttggtc gtccaaaatc
    28801 ggtttcgtac ttgctgcggc aggttcggcc atcggtttgg gcgcgatttg gaaatttcct
    28861 tatacggcgg gcaccaacgg cggcgcggtg tttttcctgc tgtttttgat atttaccgtc
    28921 ttggtcgccc tgcccgtcca gcttgccgaa ttttatatcg ggcgcacggg cggtaaaaat
    28981 gccgtcgatt ccttcagggt tctgcgtccg ggtacgcaat ggctttgggt cgggcgcatg
    29041 ggcgttgccg cctgctttat tttgctgtcg ttttacagcg tggtcggcgg atgggtattg
    29101 aattatgtcg tccacagttt tacaggagca atccatgcgg gagcggactt tgaagccctg
    29161 ttcggcacaa cgatttccaa tccggcaggt tcgctgtcct atcaggcact gtttatgctg
    29221 attacggttt gggtggtcaa aggcggcatt tcagacggca ttgaaaaggc aaaccgttat
    29281 ctgatgccgg ggctgtttat cctctttatt gcgctggctg tccgttcgct gacgctgccg
    29341 gatgcaatgg agggcgtgtc tttcctgctc aaaccgaatt ggtcgtactt taaagccgat
    29401 acgatgatta cggctttggg ccaggcgttt tttgccctga gcatcggcgt ttccgccatg
    29461 attacctacg cttcgtattt gggaaaagat caggatatgt tccgttccgg ccatacgatt
    29521 atgtggatga acctcttggt ttccctgctt gccggcctgg tgattttccc ggcggtattc
    29581 gccttcggtt ttgaaccgaa ccaggggccg ggattgattt ttatcgtatt gcccgcagtg
    29641 ttcatgaaaa tgccgttcgg tactgtttta tttgcagtat tcatgcttct ggtcgttttc
    29701 gccacgctga cttcggcatt ttcgatgttg gaaacggtca ttgcctcaac catccgccaa
    29761 gacgagcgca aacgcaaaaa acatacttgg cttatcggca cggctatttt cattgtcggc
    29821 atcccgtccg cgctgtcttt cggcgcatgg ggtgagttta aggttttcgg caaaaccatt
    29881 tttgatttgt gggactatgt tatttccgcc gtcattatgc cgattggtgc tttgagtgtt
    29941 tccatcttta ccgcctggat tcaggacaag cagtctgtgt taaaagatgc cggcgcgggc
    30001 agcaccgtac cacgggcagt gctgctgctg tggctgaata ccttgcgcta ccttgccccg
    30061 attgccatta ttattgtttt cgtcaattct ttaggcatcc tttaaacata ccgcccgacg
    30121 gcaaaatgcc gtctgaaagc ctttcagacg gcattttgct tcagattcaa cctatttcgt
    30181 tcaaagtata gtggattaaa tttaaatcag gacaaggcga cgaagtcgca gacagtacag
    30241 atagtacggc aaggcgaggc aacgccgtac cggtttttgt taatccacta tattgtccgg
    30301 catggccagc ctaactttaa gccgcctgct ttcggcaacc gaccgtatca gcggcaaatc
    30361 catcacggaa aacgccgtcg acaaggcatc ggcaaccgcc gcatcatccg ccattacgct
    30421 catgctttta taacgcggcg tactcacgcc ggttcgcgga tcgaacaaat gggtaagcct
    30481 gcccgctttg tccatcaccg tcccatagcc gcccgaagtg gcaaacgcct tgtctttcat
    30541 cgtgatattt gccaacacgc cttcttcgtc gtccggattg cggatgccga cattccacat
    30601 ccgcctgccg tttgtatcaa accccctgat ttcgcccata tcgaccagcg cggcaggcac
    30661 tccgtttgct ttaagcagcg caacaacctt gtccgtgata tagccttgcg cgatgccgtt
    30721 caaggacaaa cccataccct tttcggcaaa acggatttca cggtcgtcaa acgagacttt
    30781 gccgaagccg acccgtttca gggtttcctt cacgctattc tccggcggcg tttccgcatc
    30841 gggacgggcg gcaaaataat cggcatacag tttccacaac gcctgaaccg tcggatcgaa
    30901 cgcgccttcc gtcagcgcgt gaatatcgcg gccaggctca acagttccaa aaaatccgcc
    30961 ggaggcgaag tcagataacc gtcgcggttc agacggctga tcaggctgtc ttcacggtaa
    31021 aggctgaaca ttttttccaa acgcgccact tccgccaaaa ccttattgac caaatccgcc
    31081 gcctgtctgt cgtccacgcc gaacaggcgc agctccgcgc cggaacccag cgcgacacct
    31141 ttccagaaaa acacattttc atcgcgtttt ttatccatgt tgcgtttttc gccgccggcg
    31201 gcaaaaggat tcggcaaaaa agaaaccgcc gcaccggcca cagcaacggc ggcagccgtc
    31261 agaaaacgtc tgcgcccgaa atgcctgccc ataccgcctc ctaaaccgac actaccgcct
    31321 tgatgtgcgg atgcggatcg tagccttcca actcgaaatc ttcaaacttg aaggcaaaca
    31381 aatccttgac ttcaggattg attttcatca caggcaaggc gcgcggttcg cgttccaact
    31441 gcaatgcggc ctgttcgaaa tggttacggt acaaatgcgc gtcgccaaac gtatggacaa
    31501 actcgcccgc ctccagcccg cacacttgcg ccatcatcat cgtcaacagc gcgtagctgg
    31561 caatattaaa cggcacacca aggaaaatat ctgcactacg ctggtaaagc tggcaggaca
    31621 gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca gggcggcaag gccatctcat
    31681 cgaccaaagc cggattccac gccgacacaa tcaggcggcg cgagtcgggg tttttcttaa
    31741 tttgttccac cacattggcg atttggtcga tatgcctgcc gtcgggcgcg ggccagctgc
    31801 gccattggta gccgtaaacc gggcccaagt cgccgttttc gtccgcccac tcgtcccaaa
    31861 tggaaacatt gttatccttc aggtatttga tattggtatc gcctttgagg aaccaaagca
    31921 gctcgtggat tatcgagcgc aaatgcagct ttttggtcgt cagcagcgga aaacctttgc
    31981 ccaagtcaaa acgcatctga taaccgaata cggagcgcgt acccgtaccg gtgcggtctg
    32041 atttgtccgt accgttgtcg aggacgtggc gcatcaagtc caaataggcc ttcatcatca
    32101 tctttcatca aattaaacgg cacatattgt aacatttccg gccaacaccc gagtccggac
    32161 aaacgcgcag gccgccccgc cggacgctca aaacgcggat gaaggcaggc aggattgttg
    32221 gcaatttcag ccctttttca cagcaaaagc aggcgtgaaa acaaaattat cttgattgga
    32281 atcaaaaaat ctagtttaac tacttagaat aaaattccaa taatattttt tatttgagaa
    32341 attaatttat gattattggt tgattatcgg caataaacat catatatcga aaatatggga
    32401 aaaataatgt caacaatttg tgcaaaatta ggcttggcat cagaaaaaaa taggtttata
    32461 ttgccaacta caaatttgtt ttcccattag tacaatatca accaaaagga gtatccgaat
    32521 gactgacctg aacaccctgt ttgccaacct caaacaacgc aaccccaatc aggagccgtt
    32581 ccatcaggcg gttgaagaag tcttcatgag ccttgatccg tttttggcaa aaaatccgaa
    32641 atacacccag caaagcctgc tggaacgcat cgtcgaaccc gaacgcgtcg tgatgttccg
    32701 cgtaacctgg caggacgaca aaggacaagt ccaagtcaac cgcggctacc gcgtgcaaat
    32761 gagttccgcc atcggccctt acaaaggcgg cctgcgcttc cacccgaccg tcgatttggg
    32821 cgtgttgaaa ttcctcgctt ttgaacaagt gttcaaaaac gccttgacca ccctgcctat
    32881 gggcggcggc aaaggcggtt ccgacttcga ccccaaaggc aaatccgatg ccgaagtaat
    32941 gcgcttctgc caagccttta tgaccgaact ctaccgccac atcggcgcgg acaccgatgt
    33001 tccggccggc gacatcggcg taggcggacg cgaaatcggc tacctgttcg gacaatacaa
    33061 aaaaatccgc aatgaattca cttccgtcct gaccggcaaa ggtttggaat ggggtggcag
    33121 cctcatccgc cccgaggcga ccggctacgg ctgcgtctat ttcgcccaag cgatgctgca
    33181 aacccgcaac gatagttttg aaggcaaacg cgttctgatt tccggttccg gcaacgtggc
    33241 gcaatacgcc gctgaaaaag ccatccaact gggtgcgaaa gtgctgaccg tttccgactc
    33301 cgacggcttc gtcctcttcc ccgacatcgg tatgaccgaa gcgcaactcg ccgccttgat
    33361 cgaattgaaa gaagtccgtc gcgaacgcgt tgccacctac gccaaagagc aaggtttgca
    33421 atactttgaa aaccaaaaac cgtggggcgt tgccgccgaa atcgccctgc cctgcgcgac
    33481 ccagaacgaa ttggacgaag aagccgccaa aaccctgttg gcaaacggct gccacgtcgt
    33541 tgccgaaggc gcgaatatgc cgtcgacttt gggcgcggtc gagcaattta tcaaagccgg
    33601 catcctctac gctccgggca aagcctccaa cgccggcggc gtggcaactt cgggcttgga
    33661 aatgagccaa aacgccatcc gcctgtcttg ggcgcgcgaa gaagtggaca gccgactgtt
    33721 cggcatcatg caaagcatcc acgagtcctg cctgaaatac ggcaaagtcg gcgacaaagt
    33781 gaactacgtc aacggtgcga acatcgccgg cttcgtcaaa gttgccgatg cgatgctggc
    33841 gcaaggcttc taagcaaacg cctccgcccc gcaaacaaaa tgccgtccga accgcaaatg
    33901 ctgttcagac ggcatttcat tatccgcccg ttcgaatcgg gtaatactat agtgaattaa
    33961 atttaaatca ggacaaggcg gcgagccgca gacagtacaa atagtacggc aaggcgaggc
    34021 aacgccgtac tggtttaaat ttagttcact ataccgatac atccgaatat gccagaccgt
    34081 ctgaacggta ttcatacctc ccaatcctgc acgcgcttca aatcgttttg cgccaaagta
    34141 tccgcgtggc ggttacggct ctgatattcc ctgtctttca atatgctgct cgccacataa
    34201 ttcaaatgag cctttgccgc ctccgaagcc tcgcccggcc ggcggttcga tatcgcctca
    34261 tacagcacgc ggtgctgcgc catcagtttc ggacgcggat cttcttcctg attcagataa
    34321 ataaggctgc tgcgcgtctg ccggtacagc attttcaaca aaccgcccga caaatggctg
    34381 aacaacaaat tgtgcgccgc atccgcaatc gtctgatgga agctgacatc ggcttcgccc
    34441 tgatgttcca gattgccgct tccgcacgct tcctcaaact tttcaagcca aaaccgaatc
    34501 cgcttcaaat cggcatccgt ccggcgttct gccgccaatg ccgccataca gccctcgatg
    34561 tggcagctga aatcaaaaac atcctgttcc caattggaat gtttgcccaa aagctcctgc
    34621 caactttgca aaaaatcctg ctgcggcttg accgaaacat aataaccatc gccctgcctc
    34681 gcttccaaaa tctgacgggc aaccaaaata ttcaatgccg accggaccga cggacgcgaa
    34741 acgccgaact cttccgccaa aacgcgttcg ggcggaatct tgcccccttc cgcgtaaacc
    34801 ccttccacga tgcggccttc caataccgac aatacctgat cgctgatttt ctgaggcctt
    34861 accagtttca tcactcctcc tttataaaga ttccctgcag aacccttccg aaatacatca
    34921 aacatcaaat tggactggcc aatcagggcg gattctaatg acacgccgtt cccgccgtca
    34981 acggcactta cctcgcaccg cccccgaaac acaggaaaaa actacaccaa actacaattt
    35041 ttgttcatgc aaatatttgt tttaacggga tttaaatgaa agctccgatt caaatctgcc
    35101 gaaccgccca aaaatatatt gacctgaata ttaaagtttc gtaaagtaat gcaacgttgc
    35161 tttaattggt ttgaccacta ttgccgacga ttagaaaaat attttcggag atgttcaatt
    35221 atggaaactt gggttcaaaa ctacacggca atcggcggca gcctgtatct gactgccgcc
    35281 gccgcactct tacccatcgt ctttttcttt gccgcgctga ccgtcctgaa gctgaaaggc
    35341 tatcaggcgg ggctttatac gctgctgatt gcgcttgccg ttgccgtatt cggcttcggg
    35401 atgccgacgg gtatggcggt ttcttccctg tcgccgcaac cgcattgacc caacgcccca
    35461 tacgctacac tggacttgac cgcgcattac aaaatcggca aatccacccg catcggtttg
    35521 gactttgaaa acgtctttaa caaacgctgc cgcacgatgc ccgacattca cgtttacggc
    35581 acgccgcgca gcctgaccgc aaccgtcaaa catatagtgg attaacaaaa atcaggacaa
    35641 ggcggcgggc cgcagacagt acaaatagta cggaaccgat tcgcccggtg cttgggcgcc
    35701 ttagggaatc gttctctttg agctaaggcg gggcaacgcc gtactggttt ttgttaatcc
    35761 actataaaga aagaaatgcc gtctgaaacc ttatcgtttc agacggcatt tgtttgcccg
    35821 ggcggttatt tgccggtttg ggtatcccgt ttcaatccgc cgccgagcac cttgtacaaa
    35881 tcggcaaggt tttcggcgcg ggtcagttgt gccgacaaag ccgcaccttc cgccgaatag
    35941 ctgatgcgtt ccgcatcgag caaatcgagc gcgccggata cgccgtgttt gtaacgcagt
    36001 ccgaccaagc gcaacgcttc tttagaggcg cggctttgtt tgcttaaagc gtcataggct
    36061 ttatccagct gctcgcgcgc cgccaatgcg tttgccacgt cttgaaaggc ggattggacg
    36121 gcggattcat aggcaacaat ttgtgcctgt tggcgcagtt ttgccacatc aaggttcgcc
    36181 ttgttcgttc cccaagtaaa aatcggcagg gtaatagacg gcgcgaacgc ccaaacgccc
    36241 gtgccgcttt tgaacagccc gcccaattcg acagaacccg taccgacgct tccggtcagg
    36301 cggatggacg ggaaaaaggc ggcgcgcgcc gcaccgatat tggcgtttgc ctgtttgagc
    36361 gcgtgttcgg cggcgcggat gtcgggacgg tcgagcaata cttcggaact caaaccggca
    36421 ggcagttttt caacaaaaaa ctgcttgtcc aacggcaaac cggcgggcag gtcttcgggt
    36481 atcggacggt taatcaaggt tgccaaggca ttgcgcgcct gttcgcggct gcgcgcggca
    36541 tgggcataat cggctttggc agattcaatc aaggcttcct gctggcgcag ggcgacggcg
    36601 gaaatcacgc ctgccttgta ccgcaattcg gacagcttgt aggtttcctc gcgcgttttc
    36661 aagacacgct gcgccaaaga catcgcttct tcggcataac gctcgttgaa ataggctttg
    36721 gcaacggtgg caatcaggct caaatgtgcc gcatcgcggt tggcaacgct ggcaaaatag
    36781 ccttgcagtg ctgcttcgct gctgctgcgc acgcgcccga acagatcgag ttcgtaagat
    36841 gccgcaccca gtccgacatt gtagctgctg ctgacattgc cgccgctcaa gctgccttgg
    36901 cgcgagccgt tcgcattggc ggcaagcgtg ggcaggaggt tgttgcgctc gatcatgtat
    36961 tgtttgcggt agatttcgct gttcaatacg gctgtacgca aactggtatt gcgctcgagt
    37021 gcgatgtcga tcagcttttg caggcgcggg tcggcaaaat agtcatgcca acccaaatca
    37081 accgcgcgga tggaagaaac cgatgtgtcg ttttggaagg tttccgcaac ttcgactttg
    37141 ggctgctcgt attgaggaat catggtgcag gcagacaatg caaaggctgc tgcaacagag
    37201 gtcaaggtag ttttcaatgt agtattcata aaaaagtcct gatgccgtct gaaaaccggc
    37261 ggttctcaga cggtattttg cttaatattg tttatcgtcc gaaccggtta tacccgcttt
    37321 tgatgcatgt ctgaccgcca tttcatgctc gtgtgcagtt tctttgaaga atttgcgcac
    37381 cactacataa aaaagcggaa caaggaacac ggacaagagc gtgccgatga gcatccccca
    37441 gaatacggtc gtgccgatgg cgcgctggct ggcagaactc gcaccagcgg caatatacag
    37501 gggaaccacg cccaaaataa aggcaaacga ggtcatgata atcggacgga aacgcaggcg
    37561 ggctgcctcc aaagcggctt cgaccgcgct tttcccttgc gcttgaaggt ctttggcaaa
    37621 ttcgataatc agaatcgcgt ttttcgcact caaacccatc acagtaacga aaccgacttg
    37681 gaagtagatg tcgttggcaa acgagggaac gctgcccaac agtccttcaa acaggttgcg
    37741 cccggttacg cccgcagccg caccgatcaa acccaacgga atcacaagga tgaccgccag
    37801 cggaatcgac cagctttcat aaagcgcggc aagtaccaaa aatacggctg caaccgccaa
    37861 accgtacaaa atcagggttt gcgagccgcc ttttgcctct tcgcgcgact gtccgcccca
    37921 ttcgaagctg taaccgccgc ccaattcgtc aaccattttt tgaaccgccg ccatagcctg
    37981 cccggtggaa acgccggttg caggcgaagc ggacagcttc atcgagggat aaccgttgaa
    38041 gcgtacgctc tgttccgtac cgttttccca agaaacagta gcaatggtgg aaagcggtac
    38101 ggcgacgccg gatttgttcg gcacggtcag gttcaaaata tcggcaggct gcatacgggc
    38161 atcctcgtcg gcctgcacca tcacgcgttg cagacggcct tggttcggga agtcgctgac
    38221 ataagacgaa ctcagcgcgc ttgccaatgc ggtgcggatg tcggcaaacg aaatgccttg
    38281 cgccgccgct gcggcacggt tgatgtcgat tttcaactgc ggcgagtctt ccaaaccgcc
    38341 ggcacggacg gtgctggggt caaacaaacc gctggcacgc attttctgaa tcagctcgtt
    38401 gcgcttcgcc agcaatgcgg tatggccggt attgttgcgg tcttgcaggt tgatgctcag
    38461 gcccgaaccg ttgcccaact ccagaatcgg aggcgggacg acggcgatgc cgaaaccgtc
    38521 tttaagcgtc cccatcatca tacccgtcag cttgccggca accgcaacgg catcgctgcc
    38581 ggaggcggta cgttcgttcc aatctttcaa tatggcaaaa cccatcgcca tattctgacc
    38641 gctgcccgaa aagctgaagc cggaaacggt aatgatgttt tcaatttcgg gaatgctttt
    38701 cgccagttgg gtaacttgtg ctagcgtcgc atcggtgcgc tctttggtcg cacccgcagg
    38761 cagttgcacg ctgaccataa cgaagccttg gtcttcggtc ggcaggaacg aagtcggcag
    38821 gcgcataaac aggaacacgc ccacaaccgc caagccgata tagacaacca tcatgcggaa
    38881 agtcttacgc agcactttgg caacccggcc ttcgtagccg tgcgtccaac tgtcgaattt
    38941 cttgttgaac cagccgaaga aacctttttt ctcttcgtga tgccctttcg ggattgtctt
    39001 caacattgtg gcacacaaag caggggtaag ggtcagcgca aggaaggcgg agaatgcgat
    39061 tgatgacgcc atcgtcaggg caaactgttt gtaaatattg ccggccgccc cgctgaacat
    39121 cgccagaggc acgaacacgg aaatcagaac ggcggtaata ccgatgaccg cgcccgaaat
    39181 ctgacccatc gcttttttgg tcgcttcttt gggcggcagg ccttcacccg ccataatgcg
    39241 ctcgacgttt tcaaccacca caatcgcgtc atcgaccacg atgccgatga ccaatatcat
    39301 cgcaaacatc gtcaatacgt taatcgacat ccccatataa gagatgaagg cgaaaccgcc
    39361 caacagggaa atcggtacga cgatggtcgg aatcagcgta taacggatgt tttgcaggaa
    39421 gaggtacatt acgacaaaca ccagcaccat cgcttcgatt aaagtgtgaa tcactttttc
    39481 aatcgaaatt tcaacgaatt tggaagtatc gtaaggggtt ttccaactca taccctgcgg
    39541 aaagtatttt tccagcaccg ccaagcgttc ttttaccgcc tttgccgtcg ccatcgcatt
    39601 gccgctgttg gacaacatta ccgccatacc ggtggtattt accccgttca ggcgggttga
    39661 ggaagaatag tcttccatac ccaatccgac tttcgccaca tccttcaggt aaatattaga
    39721 accgtcggta ttggcgcgga ggatgacgtt gccgaattct tctgccgtac ccaactgccc
    39781 ttgcgccgtt acggtagccg taaccgtctg tccgcgcaca gcgggaagcg aaccgatgga
    39841 acctgcggaa atttggatat tctgcgccga caacgcgctg ccgacatcgg caaacgacaa
    39901 attgtagttt tgcagtttct taggatcgac ccaaatccgc atcgcgcgtt gcgcgccgaa
    39961 caggcggacc tgccccacgc cttcgatacg ctgcaattcg ggaacgacgt tgcgctgcgc
    40021 gtagtcgttc atctcttcgg ttgactgcac atccgacgaa agcatcacaa tcatcaggaa
    40081 attggaacgc gccttggata cggttacgcc gtattgctgg acggttgccg gcagcgtgct
    40141 caatacttcg gaaagcttgt tctgcacttc cacctgcgcc agattctcgt cggtatcggg
    40201 cgtaaaggtc aggctcacgc tgccgctgcc gctcgaatcg gcggaagtgg acatatagtc
    40261 caaaccttcc acgccgttca tattccgttc gatcacggaa agtacgctgc cttccatcac
    40321 ttgtgcggac gcacccggat aaatggcgtg caaagtaatg gtcggggcgg caacggacgg
    40381 atattgcgaa accggcaggc ttttaatgcc gaaaatacct gccgcaataa tgaaaatcga
    40441 aataacccac gcgaaaatgg ggcggtcgat aaagaattta gccatcgatg ccttccttat
    40501 ttcgcttcag aagcaggttt ggcttcagat gccgtctgaa cgccggcttg aggggcggca
    40561 gcttggtttt cagacggcgc ccattcttta ggcgttacct ttttcgcccc cgtcataccg
    40621 gcgatactga tgccttccac aaccaccttg tccccgtcct tcagacccga cgtaacaatc
    40681 caattcgtac cctgctgttg cgcgaccgtt acctcgcggg gttccatacc gccctgggca
    40741 ttcacaatca tcacggtatc tttcgcaccg cgcgttaccg cctgctgtgg aacgatgaat
    40801 gcattatccg ccgccacctg atccatcagc acacgcacat acaggccggg catcaggata
    40861 ttctgatcgt tcgatacggc ggcgcgcaag gtaatctgac cggtcgattc gtcaacggtc
    40921 ggatcagcaa acagcaggcg gcctttttca ggataaaccg taccgtcgtc aaatttgatg
    40981 ccgaccgcaa tcgcaccatc cgccgccagc agcttgcctt cggctatctg ccggcgcagt
    41041 ttcatcactt cggatgcaga ctgggtaacg ttcacataca tcggattggt ttggcggatg
    41101 gtggctaaaa cagttgtatc gcccgcattc aacagcgtac cttcggaaac tttggactga
    41161 ccgataaagc ccgaaatcgg cgcggtaatg cgcgaacggt tcagattgat gccggcggat
    41221 ttgatcgccg cctgcgccgc tttaacgccc gcctcggcag aacgtttcgc cgttaccgca
    41281 gcatcgtact cttgtttact gatggcatcg gcggaaacca gcggtttgta acgcgccaaa
    41341 tccgcatccg ctttggcaag cgttgcctgt gccgttgcca gttgcgcgcg cgcgctttcc
    41401 agacctgctt cataagtgga actgtcgatc tgatacagcg gctgcccggc gcggacataa
    41461 ctgccttctt ggaacaggcg tttttggatg atgccgccga cttgggcgcg gacatcggcg
    41521 gtacgcagcg attccaaacg ccccggcaac tcgacggtca atgcgacggt ttgcggatgg
    41581 acggttacga cgccgacgac gggcgcgggg gcttcccgac ccgcaggctg cccgccctgc
    41641 gccgcgtctc cgcctttacc gcaagacgac agtgccaatg caacggcggc agccaacgcg
    41701 gccgcacgca tcgccttaga agcataaaaa gccattattt atcctatctg tctggtttga
    41761 tgtaaagggt tttgccaatc aacaggcatt cttatttcag gatataaaaa ccgcctgctt
    41821 tgatacccga atgttcgaac gggttgcaaa gcaggttata cctgttttca aagttgagat
    41881 gcagtctcaa ttttatgggt ttcattatac atacacgatt gcacggataa aaagtctttt
    41941 ttataatccg ccctcgtcaa accgacccga aacgaaaacg ccattatgag aaaaaccaaa
    42001 accgaagcct tgaaaaccaa agaacacctg atgcttgccg ccttggaaac cttttaccgc
    42061 aaagggattg cccgcacctc gctcaacgaa atcgcccaag ccgccggcgt aacgcgcggc
    42121 gcgctttatt ggcatttcaa aaataaggaa gacttgttcg acgcgctgtt ccaacgtatc
    42181 tgcgacgaca tcgaaaactg catcgcgcaa gatgccgcaa atgccgaagg agggtcttgg
    42241 gcggtattcc gccacacgct gctgcacttt ttcgagcggc tgcaaagcaa cgacatctac
    42301 tacaaattcc acaacatcct gtttttaaaa tgcgaacaca cggagcaaaa cgccgccgtt
    42361 atcgccattg cccgcaagca tcaggcaatc tggcgcgaga aaattaccgc cgttttgacc
    42421 gaagcggtgg aaaatcagga tttggctgac gatttggaca aggaaacggc ggtcatcttc
    42481 atcaaatcga cgttggacgg gctgatttgg cgttggttct cttccggcga aagtttcgat
    42541 ttgggcaaaa ccgccccgcg catcatcggg ataatgatgg acaacttgga aaaccatccc
    42601 tgcctgcgcc ggaaatagtc aagccttggt aacaatgccg tctgaaacga acaaaccctt
    42661 tcaaacggca tcaaaatgac acaaagcatt cttctaaaaa tacatattca ctaaattgca
    42721 tttttaattt cccctatcat cgcatgaaca ttgtcttggt caaaatgtcc gttttcttct
    42781 gaataaactt ctaacaaata atgttcaatg aacgttttat ctgtcatcaa cgatacatct
    42841 ttggcaatgt cttcatacga ctcaaaatca tcttcatgcc atggatcata tttatccatg
    42901 attttttgaa tttcattttt catatcattt accttccaat atttatttac aattaataac
    42961 aataccattc gaatgtaaac tgcatttttc tccggcattc ttgcaaacaa aaaccgaaaa
    43021 tcccgtcatt cccgcgcagg cgggaattcg tttttttgag ttccagtcat tcctgataag
    43081 gctttaacgt caagttttcg gattaccgcc tttatgagaa taacgatgtg ggcattttcc
    43141 gttttaatct attacggtta tatacatata cgattatttt agtttgctta caaaacactt
    43201 catgttaaat tcaaaaattt aatgcactca atatattttt ttaaggagaa gcagatgagt
    43261 caaaccgacg cgcgtcgtag cggacgattt ttacgcacag tcgaatggct gggcaatatg
    43321 ttgccgcacc cggttacgct ttttattatt ttcattgtgt tattgctgat tgcctctgcc
    43381 gtcggtgcgt atttcggact atccgtcccc gatccgcgtc ctgttggggc gaaaggacgt
    43441 gccgatgacg gtttgattca cgttgtcagc ctgctcgatg ccgacggttt gatcaaaatc
    43501 ctgacgcata ccgttaaaaa tttcaccggt ttcgcgccgt tgggaacggt gttggtttct
    43561 ttattgggcg tggggattgc ggaaaaatcg ggcttgattt ctgcattaat gcgcttattg
    43621 cttacaaaat ccccacgcaa actcactact tttatggttg tttttacagg gattttatcc
    43681 aatacggctt ctgaattggg ctatgtcgtc ctaatccctt tgtccgccgt catctttcat
    43741 tcgctcggcc gccatcctct cgccggtctg gctgcggctt tcgccggcgt ttccggcggt
    43801 tattcggcca atctgttctt aggcacactc gatccgctct tggcaggcat tacccaacag
    43861 gcggcgcaaa tcatccatcc cgactacgtc gtaggccctg aagccaactg gttttttatg
    43921 gtaaccagta cgtttgtgat tgctttgatt ggttattttg ttactgaaaa aatcgtcgaa
    43981 ccgcaattgg gcccttatca gtcagatttg tcacaagaag aaaaagacat ccggcattcc
    44041 aatgaaatca cgcctttgga atataaagga ttaatttggg caggcgtggt gtttgttgct
    44101 ttatccgccc tattggcttg gagcatcgtc cctgccgacg gtattttgcg tcatcctgaa
    44161 acaggattgg ttgccggttc gccgttttta aaatcgattg ttgtttttat tttcttgttg
    44221 tttgcgctgc cgggcattgt ttatggccgg ataacccgaa gtttgcgcgg cgaacgggaa
    44281 gtcgttaatg cgatggccga atcgatgagt actttgggac tttatttggt catcatcttt
    44341 tttgccgcac agtttgtcgc attttttaat tggacgaata ttgggcaata tattgccgtt
    44401 aaaggggcgg tgttcttaaa agaagtcggc ttggccggca gcgtgttgtt tatcggtttt
    44461 attttaattt gtgcttttat caatctgatg ataggctccg cttccgcgca atgggcggta
    44521 accgcaccga tttttgtccc tatgctgatg ttggccggtt acgcgcccga agtcattcaa
    44581 gccgcttacc gcatcggtga ttccgttacc aatattatta cgccgatgat gagttatttc
    44641 gggctgatta tggcgacggt gatgaaatac aaaaaagatg cgggcgtggg tactctgatt
    44701 tctatgatgt tgccgtattc cgccttcttt ttgattgcat ggatcgcctt attctgcatt
    44761 tgggtatttg ttttgggcct gcccgtcggc cccggcgcgc ccactttgta tcccgcacct
    44821 taaacacgat aaacaaaata ccgtctgaaa accttttttc tgttttcaga cggcatttgc
    44881 ctttccatcc cgtcaggctt ctccggtccc ttccctcttt tccgctgcgg caagcgtgtc
    44941 ggcgagcagg cgggcgaggt tttcaaacag gggctgttcg agcgggtttt ggcttgcgtt
    45001 gccgaacacg agggcgactt cggtatagtc tttctgccct ttgctgactt tgctgccgta
    45061 ataggcggtt tgggcttttt tcagggcggc ttcccaatct tgtttttggg caaacgcttc
    45121 ggcggcggca agcgaggttt tggcaaaaaa cggcagcggg cggttttgcc cgatattgaa
    45181 aaacgccatc cattccgaca acagctgcaa tgccctgtct tgcgcgattt ccgccaatac
    45241 ttctgtttct ccgaattgga cgataaaggt ttgccgcgtt tcggcttcag acggcataac
    45301 ggcgcaaaat atcaggtgtt ccaacaaaaa agccacacgt tgcggcgcat tgggtttgcc
    45361 gtaggcgtaa aacacctgac cgcagcggta cagattgccc aagctgcctt tcaggatttg
    45421 cccgtccgac ggtatggcgt atgggaacgg aggaagtttt gggctgttta aaaccgacgt
    45481 gtcgatttgt ttggcggcgg tttggaagtc ctgctgccaa agtctgccca actctcccga
    45541 cggcaggagg ctttccgccc cgatgcgggc ggcggtttgg gaaaaatctt gtccttcgcg
    45601 gcgcgccccg atataggttt cggcgatttg ctccgcgtgt tgcggctcga agggttcggc
    45661 aggctcccag gcttcgccga tatggggttc gctccacgca agctgctgct gaagccatac
    45721 tttgacgggg ttgcgccaga agcggataaa ttcgtcctgt ccgatttcgg caacgggttc
    45781 ggcgttttct acgggttggt cgaaaaaagg gcgcggcggt tcgggcgttt gtccgagcgc
    45841 ggcggcgtag tcggtacgcg tgccgaatat gccgtctgaa cgtccgcctt cttggaaata
    45901 tcggcgcgag aaggcttgca gcggatgctg ttctatccaa ttttgtgcaa gttggcggct
    45961 accaatgccc gccatagcgg caacggtatc gatgagttcg cccaacaaag aagacggagc
    46021 cagttcttcg tctttgcgga tgtcgcgccc gatgtaggac aaatacagga tttcgcgcgc
    46081 gctgatgagg gcttcgagga acaggtagcg gtcgtcgtcg cggcgggcgc ggtcgccttt
    46141 ggcgggatgt ttggcaacca ggtcgaatac ggcggctttg gtattgcggg gaaaatctcc
    46201 gtcgttcaaa cccaacaggc agatgacttt gaacggcagg ctccgcatcg gcaccatact
    46261 gcaaaaggtg atgccgccgc gtaaaaagcc tgcctcgctt tcgctgtcga ggaagcggcg
    46321 gatgtggcgg atgacggtgt gcggcggcaa ctgtccggaa aattgcgcca attcggtttc
    46381 cgcctgccat ttgacccatt cgttttcgag gttttggact gccttttggt catcggctcc
    46441 ggcttcgaac aatctgtcgg gcaaatcccg gcaacgcgcc acccattctc cgaccgtcgc
    46501 gggctgccgc catatccggg caatatccga cagggtttcg aggaaggcgg caaaacgtcc
    46561 gaacagggcg gtttggttca cgtcggcata ccacgcgctg acatcctgcc acatcgggtt
    46621 gccgccttcg ggcagcatcc agcccaatac catacggtct gccgcctgct tccaggtaaa
    46681 cagctgatcc gtgccgccgc gcattgcgcc gtccaatccc caatggacgt tcaaatcggc
    46741 aaccatatcg tgcaaaagcg gcaaatcgtc cccggtcagt ccgaaacggc gcaacacggg
    46801 cgcggtttcc aaaagcgcga gcactttgcc gacttcaaaa cggctttcca gcaagtcaga
    46861 cacgcactcc aacgcataaa aaaacggttg ccggcggcta attttcacgt ccgaaacgga
    46921 atacggcaat gcctgcgcgc cgggctgcgc ctgtccgaac acggcttcga taaaaggcgt
    46981 atagggttcg atgttcgggg ttaatacggc gatgtcgtgc ggctgccaat cgggatgttc
    47041 atgcagaatt ttcaacagtt tgtctttgag tatctgcaat tcgcgcaagg ggctgtgtgc
    47101 ggagacgatg cgtatcgagc cgtcgcccgt gttgacgctt cccaccgttt cagacggcat
    47161 tttcaggttt tgaatatcgg tttgcagggc gtgcaaaagc gtatcgcgcc cgccttcttc
    47221 aaataccggc gtttcttctt ctatttccat ttcgttcaaa aagtcgaaaa agtcgcgccc
    47281 ctgcttgccc aatgaggcaa gcagcggatg ccctgcctgc gtcagatcgg gatcgccgcc
    47341 gcctttgagg atttgcgccg cttcgatgac gttgccccaa tgcatcccgc tcggattgag
    47401 tgcgaacacg aacacgtcgc aatgttcgga cagcttgtgc aaaagctgca aatacatcgg
    47461 cgccatcgtg gaaatgccga acacgaaata acgctcgggc agcttatcct tgtccaacgc
    47521 ggcaagcagt ttttcccaca acgcgacacg gtgcggcgcg ctctgcctgc cgtcgtcgag
    47581 gtaacgccac agtttggact gccagatttc gtcgtcgccc aaaccgagta tcctgccctg
    47641 ctgccaagcg tctatccact gaggacggta cacgaggtat tggtcgaata tgtctgcaag
    47701 ctgtcccgca agctggtaat ccgccgattc tccgctgccc agatagtctt gcagcacatt
    47761 cctcacatct tcaaattccg ctccgttttg aaattctgcg ctgcggaaca aatccagcag
    47821 ccgccagcgc atgacttcgg gtgcaaacgg gctgagttcc ggaataccgg gaatcagttt
    47881 tttcatcaac ttccacgtca ggccggcggg caggctgaac gccaaattcg ccgccacgcc
    47941 caaatcgcgg gcgaggcagg tgttgaggta gcggcgcatc ccctggctct gcacaacaat
    48001 ctgttcgggc tgcaaggcag atttcagcgg tttgactttt tgaatgcggg caaacagtgc
    48061 agcgagcgat tcgaggcggt tggattggta caggtaaaac atggcttgct ttttcaaaat
    48121 gacagtgatg cggaattata tagcttctcg ggcaagccgc ccgcaaacaa aatgccgtct
    48181 gaacgcttca gacggcatcg ggtcatttaa accatttcct caacacagga atccgcgaca
    48241 gcagcaaggt atccaacagc caaatcacgg caatggcaag cagcgcggtc gggaaaagca
    48301 gtgcaatcaa cagcagcggc aatgccatcg cccaccagac gggcagtttg attttttgcg
    48361 ccggaggaac aatgcccgcc acgccggacg gacggcgttt ccaccacatc acgcagccgc
    48421 tgatgccgat aaaaatcacg gcaaggcaga acacgacgtt cgccaacacg ctccaccagc
    48481 ccaaagtccc catatgcagc gcaatgcttg ccgccataaa tttgccgaac gggttgtaat
    48541 cgtcaaaacg gatgtcggca agaatcttgc cgctgtactg gtcgatatgt accgtgcggt
    48601 cggcaaacgg gctgatcatg tcataactca tagaatcctg cgacaaagtc cataccccgt
    48661 cctcgccttt gggcaaattc aactgataac gccctttgaa accgatttcc cgcgcgaaac
    48721 ggtcgacggt ttccaatgtc atcggctcgc tggggttaat gccgttttca cccacagtcg
    48781 tccctgagac aggcataggc gtaagctcca aaatccacgg cacttcctta accttgccgt
    48841 cattcaatac ctcgccgtgg gtcggcacga ctgaaacggg gttcggttcg acaccccatt
    48901 tgccggccgg gaactgactc caagcctgca cgaacttgcc gccccaaata cctgcccaag
    48961 caatacccga caggcagaac aacagtaaaa tcaacgacac ccaagttcca aacgcgccgt
    49021 gcagattccg ccaccaagaa cgcgccctgc cttttggcgg cagcagcatc gctttaatgc
    49081 cgcgctgttt cgcccaccaa aggtacaagc cgctgacaac cataataatg gtcagtgaag
    49141 ctgccgtttc caaaagataa tcgcctgccg caccgagcat catatcgccg tggatttcat
    49201 ccatagtgtg ataccaaccc tgattgcgcg gcatggtgtt gaccactttt gccgtataag
    49261 gatcgaccgc aaccatggtt gccttaccct cattgttgac acggaacaca gcaaccatat
    49321 catcggcacg cggtgcaata tattggacga cggacgaagt ctccggatta atggcaccgc
    49381 gtgccgcttc tgcctgaaca gagaggggtt gcaccactgc ctgagggaaa acgtgaatac
    49441 gctctccctc tttaccggta atattggcaa acaacagcat acccaaacct gtaacggcaa
    49501 gcagggtcaa gaagggcata accaataaac cggcataaaa atgccacctc caaacgctca
    49561 gataatgccg gttattctgc tgaccggctt cagttttgat ttgtgtatcc attaatcgtc
    49621 cttttgaaat taaggctatc gtgatgatgc gcgattataa acaataaaga ctaattcttt
    49681 atgactaaaa tcaatttttt tgcaacatat gaaaaaagat tcaattttga agaaacaaac
    49741 ctatatgccg tctgaaagca tcaaacaatg ctgtccgaca cccaactgca ccattcttta
    49801 aaccatacaa aaatatcagt cggtctgact gcttaagcat caaaatcccc actttcagac
    49861 ggcataaatc aaaaacagga tttccgttca aaataaaaaa tccggattcg atatgaatcc
    49921 ggatttctgc atccgacaaa gtcggaaaac aacggttaga acttaccgcc tgactggttg
    49981 accatatagt caaccgcagc tttgacctca tcatcgctca aatcgccgcg accgcctttt
    50041 gcgggcatcg tattaaaacc ttcgatcgcg tgtttgtgca acgtgtcctt accttttttg
    50101 atgcggtcgg cccagtcggc tttggtgcct acatggggaa tacctggaat tgcattgcca
    50161 tggcaggcgg cacaaacggt ttcataaacc tgtttgccat ccgctttggc ggcaggtacg
    50221 gcttttcctc ggctttaggt tctgctgcgg caggtttggc ttcagaagcg gcttgtgctg
    50281 ctcctgcagg agcagatggt gcgggttctg ccgccggtgc aggctcggct tttttcaccg
    50341 gagctttacc gtctttattg gaaagacccc atacataagc agtcatgata tgcagtttgt
    50401 ctttatccaa gaaatgtccc caagcgggca tttggctgct gcgaccgttg gtaatggttt
    50461 cgataatgga tttttgcgta ccgccccaca accacacgtc atcagtcagg ttcggaccca
    50521 aaccttggat accttgtccc ttatcgccgt ggcaagtgaa acagttggca ggcggaccgc
    50581 tgaacaaggc ttgtccgcgc gcggcacgtt cctcatcata ctgacctttg ggttttgaaa
    50641 gggacatcac ataatgggca acgtctttca cgccttcttc gcccaaagca ggaccccagg
    50701 caggcatagt cgcaacacgg cctttttcga tggtctcgtg gattttatcg ggatcgccgc
    50761 cccacaacca atcgctatcg gtcagattcg gaaaaccttt agaacctttg gcatcagagc
    50821 cgtggcactg gatacaataa gtgttaaaca ggttttgggc gatttgcttg gcctgtgggt
    50881 cttttgccac tttttcaatc ggcatatccg caaacttggc atacagtttg ccgtattgct
    50941 catcggcttt tttgacctct ttttcatatt ggttatggct ggtccatttc agcagacctt
    51001 tgtagtcgcc gacacccgga tacataacca gataaccgat accgaacagc cacgtcaaaa
    51061 catacagcca aaaccaccag cggggcagcg gattgtcgta ttcggcaatg ccgtcccact
    51121 cgtgacccgt agtttgtact tcttcgccct tcttcggacg tttgacaaca ttttgagaca
    51181 gcagcagcca agccaaagcg ataaagctca gtaagacaat aactgcaata tatatattcc
    51241 agaaattact ggtaaattgg gatgttgcgt tcattgtttt gctccgttat cacaatatta
    51301 acggttttcg cttttcttat cttgcgcatc ttggttttca gcaaaaatgc tgtttgcggc
    51361 atcatcgtag tttttcttat tccgcctgtt gaagacgata tagagtacta acaggaaaca
    51421 gataaagatc cataccgtga aaagagcgcg aataccgtta atatccatga tgttacctta
    51481 cgtttttcaa agccagaccc aatccttgca gataggcgac tacagcatcc agctcggatt
    51541 tgtttgccaa agcctcaggc gctttcgcaa tttcctcatc actgtaagga gtacctactt
    51601 tacgcaaagc cttcatgttg gcaacggttg catcgacatc gactttattg cgtgcaagcc
    51661 acgggaatgc cggcatattg gactcaggca cgacatcacg gggattcagc aggtggatgc
    51721 ggtgccattc gtcggaatag cggccgccca cacgtgccaa atcaggaccg gtacgtttgg
    51781 aaccccattg gaacggatgg tcgtaaaccg actctccggc aacagagtaa tgaccgtaac
    51841 gctcagtttc cgcacggaac ggacgaatca tttgagagtg gcagttgtaa cagccctcac
    51901 ggatgtaaat atcgcgtccg gcaacctgca gggcattgta aggcttcacg cccggcgccg
    51961 gctgtgttgc cgccttggta aaggccaagg gcacaacttc aatcaacaga ccgacactga
    52021 ctacaagcag cgtgaacaca atcagaacgc cgattttttc ttcagccaat tgttgtaatt
    52081 tcattttggt agcctatctt tcttagtatt ttagtggtgc tgtgtttggg aaaccgcagg
    52141 gatttcggca tcgactgctt taccaccgat ggctgtgcgg tacacgttgt acgccataat
    52201 gcacatgcca ctcagataca ataaaccacc tgcaaaacgg atcatgtagt aaggcatggt
    52261 gcgttttacg gattcgacaa acgagtaggt cagcgtaccg tcatcgttca gagaactcca
    52321 catcaaaccc tgcatcacac ctgcaatcca catcgcggcg atatacagaa ccacaccgat
    52381 agtcgcaatc cagaaatgcg cctctaccag cttggtgctg tgcatctgct ctttgccgaa
    52441 cagacgggga atcatgtaat agacggaacc gatggttaca aagcctaccc agcccaacgc
    52501 acccgcatga acgtgcgcga cggtccagtc cgtatagtgg ctcaatgcat tgaccgtttt
    52561 aatcgacatc atcgggcctt caaaggtaga cataccgtag aaggacaagg atacaatcag
    52621 gaatttaaga atcgggtctg tacgcagttt gtcccacgcg ccggacaagg tcataatgcc
    52681 gttaatcata ccgccccaag agggtgcgaa caaaatcaag gacagaacca tacccaaaga
    52741 ttgcgtccag tcaggcagcg cagtgtagtg aagatggtgc ggacccgccc acatataggt
    52801 gaaaatcaac gcccagaagt gaacgacgga caggcggtag gagtaaacgg ggcgggctgc
    52861 ttgtttgggt acgaaatagt acatcatacc caaaaagccg gcagtcagga agaagcccac
    52921 ggcgttatgc ccgtaccacc attgaaccat agcatcaatc gcaccggaat agacggggta
    52981 tgacttcatc aaaccggccg ggatgctgat attgttgacg atgtgcaaaa gtgcgaccgc
    53041 caaaataaag ccgccgtaga accagttggc aacgtaaatg tgttttacct tgcgtttggc
    53101 aatcgtaccg aagaatacga tggcgtaagc cacccaaacc aaagtaatca gaatgtcaat
    53161 cggccattcc agttcggcat attccttacc ttgggtccaa cccataggga agctgacgac
    53221 ggcggcaacg attaccgcct gccaacccca aaaggtaaat gccggcagcc aaccgccgaa
    53281 aagacgggta ttacaagtac gttggacaac gtagtatgat gtgccgatca gaccgcaacc
    53341 gccaaatgcg aaaataaccg cattggtgtg cagcggacgc aggcggccga agtggaacca
    53401 aggtccgata ttagacaagt cgagggcagg agcaaaaagc tgggcggcga cgataacgcc
    53461 gaccaacatg cccacaatcc cccaaactac agtcatgatg gcgaactggc gcaccacttt
    53521 gtagttgtaa gtttgtgtgt ccatgagagt ctccatgaat tatgggaata aagattttta
    53581 tcctgccgct tccgcagcct gtttaaggtg caatccgggc aagcgtaatt ttttctaaat
    53641 ttaacatatc tgccttatta cgccaagcgg aattacattc gcgccgccgg cgaacccttt
    53701 gcttaatctg ttttttatta catataaatc atattgttat aataaattac aacccggccg
    53761 ccattgcttt tgtttccaat tttccctttt tgtggcactt tattgatgta ggttaagctg
    53821 cattttaaag gcatttaatc catcccgttt aacgatatat ttgatagtta tgattcatta
    53881 taaaatagcc ccgtcccctc tagaccatga atggcacatc ctgctgaaat tcacacaaga
    53941 taatgatttt cctatagaaa taagccttcc gaattgggtg ccgggcagct atctgattcg
    54001 ggatttttcc cgccacatca cttctatcca tgcatcctgc aacggcacgt ccatgccgct
    54061 cgaacaaatt gcaaaaaacc gctggcacac tgccgccgta cgcggcgagt ggcaaatccg
    54121 ctacaccgta tatgcattcg atttgtcggt tcgaggttct ttcctgacga cagaacgcgg
    54181 tttttttgac ggatcgtgcc tgtttttgaa agtcgaagga acggaaacgc tgccgcaccg
    54241 cttggaattg acaggcattc cacccgaatg gcgtattgcc acaacgctgc cggaaacagg
    54301 gaggcttgtc tttcagacgg catcttatga cgaattgatt gaccgacctg tcgagatggg
    54361 cttgattgaa tttttagatt ttgaggcggc aggcattccg cacacgattg ccttaagcgg
    54421 catatatccc gatttcgacc gcgacaggct ggtttcggac atcaaaaaaa tctgcgaaac
    54481 agaactggcg atgttctcct cccctgcccc atttgagaaa tatttgttcc tgctccacgt
    54541 cggcgaccat atttacggcg gtttggaaca caccgacagc accgcactgc tcgccgatcg
    54601 ccacagcctt ccgccgtacg gtatgaccga tgccgacgat gcctacacca cattgctcgg
    54661 acttttctcc cacgaatatt ttcacgcgtg gaacgtcaaa tccatcaaac ctgccgcatt
    54721 cgccccttat gacctcgaca aagaaaacta taccgaacaa ctatgggcat tcgaaggcat
    54781 tacatcctat tacgacgatt tgtttttggc acgcagccgc accatctcgc ccgaatttta
    54841 tttaaacctg ctagcacaag gcattacgca cgtacaacaa acccgcggcc gtttgaggca
    54901 gaccttggcg gaatcgagtt ttaccgcgtg gaacaaattt tacaaaccgg atgaaaacag
    54961 ccccaacgcc atcgtcagct actaccaaaa aggcgcactt gccgcattgt gccttgatct
    55021 gataatacgc aaccgaagca acggcagaca ttcccttgat acggtaatgg acaaactcta
    55081 tcgggagtgg agggacacac actcgggtat tccggaaaaa cactggcaaa tccgttgtca
    55141 ggaaattacc ggcttggatt tgacagattt ttttcagacg gcattgtaca gcaccgaaga
    55201 tttgccgctt gccgaatgcc tggcaaccac aggcgtgaaa ctgaccttcc tgccgcttcc
    55261 ccgacaacac ggcggcggat atgcggaaca cattttcccc atcccgccga cgggcgattt
    55321 cggcgcacgc ttcaaacaaa acgccgacca tatcgtcctg acccatgtct tcaacggcgg
    55381 aagtgcggaa tctgcggcac tgtgcccgca agacaaaatc attgctttag acggttatgc
    55441 ctgcaccgac tttgccgcac aatgggcccg ataccacgtc agggcaaaaa tcaatatcca
    55501 cttcttccgt gcaggcatat tgcgtcaaac cgtcttgacg gttcaggcaa cggcggcgga
    55561 tactgccatc ctacacatca cagatcgcaa cctgttggaa aactggttgt tcggttaaac
    55621 tttcagacgg cattgcacac aaaatgccgt ctgaacacca accgcaaagc aaaggaaaca
    55681 aaatggcgat tctcaaactt gacgaacatc tctacatctc cccgcaactg accaaagccg
    55741 atgcggaaca aatcgcgcaa cttggcatca aaaccgtcat ctgcaaccgc cccgaccgcg
    55801 aagaagaatc gcaacccgac ttcgcccaaa tcaaacagtg gctggaacaa gcaggcgtta
    55861 ccggattcca tcaccaaccc gttaccgcac gcgacatcca aaaacacgat gtcgaaacct
    55921 tccgccaact catcggacaa gccgaatatc ccgcccttgc ctattgccgg accggtacgc
    55981 gctgctacct cctgtggggc ttccgccggg cggcagaagg tatgccggtt gacgaaatca
    56041 tccgccgcgc ccaagcggca ggtgtgaatc tggaaaactt cagagagcgg ctggacaacg
    56101 cccgcgtctg attacaagcc gaaacgttta aaccacacct tcaagcggca ttccaccgca
    56161 acttgaaaaa gaggacggca aaccttactg ccgtcctctg tccttctccg tttttacagt
    56221 gggtatagcg gattaacaaa aaccagtacg gcgttgcctc gccttaactc aaagagaacg
    56281 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctacgg
    56341 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aattagaaat gcacattttc
    56401 attattctcg cgcaggcagg actccagact tacccatttc agtaatgttt gaaaataaaa
    56461 gaaaaatcag atgtttgtat tcccgcctgc gcagaaatgg agacggtgct ctgtcgtctc
    56521 atttttgttt taatcaacta tatataactg attaaacata agaaatgccg tctgaaagac
    56581 tttcaggcgg cattcgttca agcgtcgaac tttattgcgc cttggtttcg gttacaaaac
    56641 cgattttggt aattcctgcc tgacgggcgg cttctaaagc tttgtttaca taatcgtatt
    56701 ccaccgcctt gtctgccgca atcgccacaa tcacgttttc attctgctcc ttggcggctt
    56761 tcagacggct ttccacttcc ccgatttcca ctttgcttgc agaatccccg ccgacataat
    56821 agccgccgtt cgcatcaatc gtcaggcgca gggggtcttt aggctgtttg tcctgcttgt
    56881 ttgcctgctc ggacgcggtc ggcagttcca aagggatgga atgcgtcagc accggcatag
    56941 taatcataaa cacaatcagc aacaccagca tcacgtccac caacggcgta acgttgatgt
    57001 cggacatcgg agaatcgtcg ctggaattca tcgaaccaaa tgccataatc agctatcctt
    57061 ttgattaagc aggcggacat gcaaatcgtg cgccatcgca tccaaatcct gggtcagtat
    57121 ttttgtgccg cgattgagga agttgtatgc caacaccgcc ggaatcgcca cgaacaaacc
    57181 cgccgccgtc gccaccagtg cctcgccaat cgggccggca accgccgcaa tactcatctg
    57241 cccgctttgc ccgatattga tcagggcgtg gtaaatcccc caaaccgtgc cgaacaaccc
    57301 gataaacggc gcggtcgcgc cgatggaggc aagcgcggtc atcccgtaat caaaccggcg
    57361 cataatctgc gccatactgt tgcggatttg aatgaccaaa tactcgttca acggcaaagc
    57421 ctgcgccagt tcggacgctt cgtttcggcg gtagttgcgg taagactgca atgcctcttg
    57481 cgccagtttg gacaaaggcg catcgacggc gcgcactttt tcgaccgcgt cgttcagcga
    57541 caaagtatcg cgcatatgcc gtttgacggc ggcattccct ttgcgcgccc gatacagctt
    57601 gatgcagcgc aagacaacca gacaccacgt tacggtactc atcaacagca tcaacacaaa
    57661 cacaccgatc aggacgggat cgcccgattc aaacactaat ttcaaattca taatgattcc
    57721 aacactgaaa aaaccaatca aacatccaag ctgccgcaaa ctgccgcgac aaccgcctaa
    57781 ttcaattcaa acttgacggg gactttaaac tccgtccagg cattggcttg aaaatgcccg
    57841 ttttgcgccg ctttgcgtgc cgcattgtcc aaacgggaaa aaccgctgct tttcaccact
    57901 ttgacggact cgacatgacc gcccggagaa accaaaacgc tcaaaacaac cataccctgc
    57961 tcgtcatttt ccatagaaag tgcgggatag gccgggcgcg ggatgctgcc gttggcgcgt
    58021 aaaggattgc ctttgctgct gccgacaccg gttccgccgc cgctgccgcc ggctccttcc
    58081 ccgtgttcgc ctttggcacc gccgcttcct ttaccgctgc cttctccgcg ccccgttccg
    58141 tcgcctttgg taccggttcc cttaccttcc ccattaccct gctcgctgcc ggctttggca
    58201 gaagcattac cggaatgttc ggcaggtttt tcagacggct tctctgccgg tttttccgcc
    58261 ggtttcgggg caggcttcgc ttccggttcg ggttcgggct taggtttttc ttcgggtttc
    58321 ggcttttcct taggttgctg aatatccgca tccgcctttt ttgtaaccgc cggcttcaaa
    58381 accggcttgg gcggttcgac aggtttgggc ggatcgggcg cgggttgcgg ttcgggcgcg
    58441 gcaggcgcgc ctgcgccttc gggtgcgccg ccccctccgc caaaatcgcc caaatcgaca
    58501 aattcgataa catttcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc
    58561 actattgcca aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct
    58621 ttatccataa ttcgggcata ataatagcaa caattcctat ttgcaaccta ttttttataa
    58681 tttgttgtca tgtgaacgcc tgctccattc ataggcaaac cgtgtttaaa cgcggtatta
    58741 cagcaaatca tcagataacg ggccggcaga aaaaatgatt ccgtctgatt tcttattcca
    58801 ataaaatcag gttagatgat atattgccgc ttctgtctgt cagccgtttc gggctgcaca
    58861 ccacatctgt tcaaaggaaa accatgtttc aaaattttga cttgggcgtg tttctgcttg
    58921 ctgtcctgcc cgtgctgctc gccattaccg tcagggaggt ggcgcgcggc tatacggcgc
    58981 gctactgggg agacaacact gccgaacaat acggcaggct gacactgaac cccctgcccc
    59041 atatcgattt ggtcggcaca atcatcgtac cgctgcttac tttgatgttc acgcccttcc
    59101 tgttcggctg ggcgcgtccg attcctatcg attcgcgcaa cttccgcaac ccgcgccttg
    59161 catggcgttg cattgccgcg tccggcccgc tgtcgaatct ggcgatggct gttctgtggg
    59221 gcgtggtttt ggtgctaact ccgtatgccg gcggggcgta tcagatgccg ctggctcaaa
    59281 tggcaaacta cggtattctg atcaatgcga ttttgttcgc gctcaacatc atccccatcc
    59341 tgccttggga cggcggcatt ttcatcgaca ccttcctgcc ggcgaaatat tcgcaagcgt
    59401 tccgcaaaat cgaaccttat gggacgtgga ttatcctgct gcttatgctg accggggttt
    59461 tgggtgcgtt tattgcaccg attgtgcggc tggtgattgc gtttgtgcag atgttcgtct
    59521 gactggctct cagacggcat aaacgttcca aaaaacgcgg aaggacatat tgccctgccg
    59581 cttttttttg gttttacaat gccctgtcga aataaagcgg cacgcctgat ttttcactca
    59641 taccgattaa ataatccatc gtcttccgag ggaacgcgat gccgcgcacc acggtcagat
    59701 tcctcaaaac ggggaaaacc aaaatatcct ccataccgat tccaccgttg atgccgtctg
    59761 aagtaccgtc catcaaattc tccaactctt gcaaatctgc gtttatccgt tcaaggtatt
    59821 gggcggtttt attcaaattg gcggaaaagc tgccgatgct tttctctttt ttgtctgtga
    59881 aatatttcac cgcttccggc gttacaaatt cgggcagccc gattttgatc aagcgtggct
    59941 gaaccaattt gttgttgtat ccgcctacct tgtccagcca cgcctgtatc tcggggcgga
    60001 cttcgtcttt cagacggcct tctcggtcga aatggcacac aatgtccaaa ctctcgccca
    60061 taaacgaacc gtcttctttt tgcaggacgg gcacttgttt cgcaccgatc ataccgatcg
    60121 gcgttgcctc gtcgtcgttt gccagcacga cttcttcaac gtcagcacca aacagcccgg
    60181 cagccatccg cgcacgcacg caaaacgggc aatggtcgta aatatacagt ttcatcaaaa
    60241 tattcctcgt caacctgtcg gtaccggcta ccttaacacc cggcgccgcc cgaaacaagt
    60301 ttatcttccc gcctatgcac cgtaaataaa taagctgtta caatgaactc gtttttatcg
    60361 gaacggaaga tcccatcatg accgccatca gcccgattca agacacgcaa agcgcgaccc
    60421 tgcaagaatt gcgcgaatgg ttcgacagct actgcgccgc tctgccggac aacgataaaa
    60481 acctcatcgg taccgcatgg tcgctggcgc aggaacatta tcctgccgat gccgccacgc
    60541 cgtatggcga gccgctgccc gaccacttcc tcggcgcggc gcaaatggtc gacgaactcg
    60601 acctgctgcc cgatgccgtc gccgccaccc tgcttgccga catcggacgc tacgtccccg
    60661 attggaacct attggtttcc gagcgctgca acagcaccgt cgccgagctg gtcaaaggtg
    60721 tggacgaagt gcagaaactt acccacttcg cccgggtgga cagcctcgcc acgccggaag
    60781 aacgcgcgca gcaagcggaa accatgcgga aaatgctgct ggcgatggtt accgacatcc
    60841 gcgtcgtatt aatcaaactg gcgatgcgta cgcgcaccct gcaattttta agcaacgccc
    60901 ccgacagccc tgaaaaacgc gccgtcgcca aagaaaccct cgacatcttc gccccgctcg
    60961 ccaaccgctt gggcgtgtgg cagctcaaat ggcagctcga agatttgggc ttccgccatc
    61021 aagaacccga aaaataccgc gaaatcgccc tgcttttgga cgaaaaacgc accgaacgcc
    61081 tcgaatacat cgaaaacttc ctcgatatcc tgcgtacgga actcaaaaaa tacaatatcc
    61141 actttgaagt cgccggccgt ccgaaacaca tctactccat ttacaaaaaa atggtgaaga
    61201 aaaaactcag cttcgacggc ctgttcgaca tccgcgccgt gcggattctg gtcgataccg
    61261 tccccgagtg ttacaccacg ctgggcatcg tccacagcct ctggcagccc attcccggcg
    61321 agttcgacga ctacatcgcc aaccccaaag gcaacggtta taaaagtttg cacaccgtca
    61381 tcgtcggccc ggaagacaaa ggtgtggaag tgcaaatccg caccttcgat atgcaccaat
    61441 tcaacgaatt cggtgtcgcc gcccactggc gttacaaaga aggcggcaaa ggcgattccg
    61501 cctacgaaca aaaaatcgcc tggttgcgcc aactcttgga ctggcgcgaa aatatggcgg
    61561 aaagcggcaa ggaagacctc gccgccgcct tcaaaaccga gcttttcaac gacacgattt
    61621 atgttttgac cccgcacggc aaagtcctct ctctgccaac gggcgcaacc cccatcgact
    61681 tcgcctacgc cctgcacagc agcatcggcg accgctgccg gggcgcgaaa gtcgaagggc
    61741 agattgtgcc gctgtccacc ccgctcgaaa acggacagcg cgtcgaaatc attaccgcca
    61801 aagaagggca tccttccgtc aactggcttt acgaaggctg ggtcaaatcc ggcaaggcca
    61861 tcggcaaaat ccgcgcctac atccgccagc aaaacgccga caccgtgcgc gaagaaggcc
    61921 gtgtccaact cgacaagcag cttgccaaac tcacgcccaa acccaacctg caagagcttg
    61981 ccgaaaatct cggctacaaa aagccagaag acctctacac cgccgtcgga caaggcgaaa
    62041 tttccaaccg cgccatccaa aaagcctgcg gcacgctgaa cgaaccgccg cccgtgcccg
    62101 tcagcgcaac caccatcgtc aaacagtcca aaatcaaaaa aggtggcaaa accggcgtgc
    62161 tcatcgacgg cgaagacggc ttgatgacca cgcttgccaa atgctgcaaa cccgcgccgc
    62221 ccgacgatat tgccggcttc gttacccgcg agcgcggcat ttccgtccac cgcaaaacct
    62281 gcccctcttt ccgacacctt gccgaacacg cgcccgaaaa agtactggac gcaagttggg
    62341 cggcgttgca ggaagggcaa gtgttcgccg tcgatatcga aatccgcgcc caagaccgct
    62401 ccgggctttt gcgcgacgta tccgacgcgc tcgcccgcca caaactcaac gttaccgccg
    62461 tgcaaaccca gtcccgcgac ttggaagcca gcatgaggtt cacgctcgaa gtcaaacaag
    62521 tcaacgacct cccgcgcgtc ctcgccggcc tcggcgatgt caaaggcgta ttgagcgtta
    62581 cccggcttta aatacaaaaa tgccgtctga aagccggata acgcttcaga cggcattttg
    62641 attgccgggt tttgctattt tttgttgtaa taatcaaatc gcacgttgac tatgtctttc
    62701 tcggtaaaaa tataacggag catcgttgtg aatctttcat aacgttcatg aattcccaca
    62761 ctatcaggca accaagggga agctttaatt tcaaaaagct cccaatttgg aaccattaaa
    62821 aaatcaataa tggtaccgat tccaatcacg atgtcccttg gtatatccat cggataagga
    62881 tatttttttc taacctcgat taaagcattc tccaa
//
LOCUS       DAXFHF010000002        56980 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXFHF010000002 DAXFHF010000000
VERSION     DAXFHF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 56980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56980
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.002"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(9..83)
                     /locus_tag="ACFIRM_000061"
     tRNA            complement(9..83)
                     /locus_tag="ACFIRM_000061"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Glu,seq:ttc)
     gene            complement(101..177)
                     /locus_tag="ACFIRM_000062"
     tRNA            complement(101..177)
                     /locus_tag="ACFIRM_000062"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Arg,seq:acg)
     gene            complement(288..1961)
                     /gene="recN"
                     /locus_tag="ACFIRM_000063"
     CDS             complement(288..1961)
                     /gene="recN"
                     /locus_tag="ACFIRM_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC3027809.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2145..2747)
                     /locus_tag="ACFIRM_000064"
     CDS             complement(2145..2747)
                     /locus_tag="ACFIRM_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC3027810.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(2848..3789)
                     /gene="ylqF"
                     /locus_tag="ACFIRM_000065"
     CDS             complement(2848..3789)
                     /gene="ylqF"
                     /locus_tag="ACFIRM_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC3027811.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3870..4724)
                     /gene="rapZ"
                     /locus_tag="ACFIRM_000066"
     CDS             complement(3870..4724)
                     /gene="rapZ"
                     /locus_tag="ACFIRM_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC3027812.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(4705..5667)
                     /gene="hprK"
                     /locus_tag="ACFIRM_000067"
     CDS             complement(4705..5667)
                     /gene="hprK"
                     /locus_tag="ACFIRM_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC3027813.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5671..6120)
                     /gene="ptsN"
                     /locus_tag="ACFIRM_000068"
     CDS             complement(5671..6120)
                     /gene="ptsN"
                     /locus_tag="ACFIRM_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC3027814.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(6305..7195)
                     /gene="ubiA"
                     /locus_tag="ACFIRM_000069"
     CDS             complement(6305..7195)
                     /gene="ubiA"
                     /locus_tag="ACFIRM_000069"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC3027815.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGFAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7185..7592)
                     /locus_tag="ACFIRM_000070"
     CDS             complement(7185..7592)
                     /locus_tag="ACFIRM_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027816.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(7601..7696)
                     /locus_tag="ACFIRM_000071"
     CDS             complement(7601..7696)
                     /locus_tag="ACFIRM_000071"
                     /inference="COORDINATES: protein motif:HMM:NF026993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HFC3027817.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(7716..8192)
                     /locus_tag="ACFIRM_000072"
     CDS             complement(7716..8192)
                     /locus_tag="ACFIRM_000072"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC3027818.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(8207..8854)
                     /gene="bioD"
                     /locus_tag="ACFIRM_000073"
     CDS             complement(8207..8854)
                     /gene="bioD"
                     /locus_tag="ACFIRM_000073"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC3027819.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSYPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(8851..10152)
                     /gene="bioA"
                     /locus_tag="ACFIRM_000074"
     CDS             complement(8851..10152)
                     /gene="bioA"
                     /locus_tag="ACFIRM_000074"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC3027820.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(10370..10594)
                     /locus_tag="ACFIRM_000075"
     CDS             complement(10370..10594)
                     /locus_tag="ACFIRM_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027821.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            complement(10786..11271)
                     /locus_tag="ACFIRM_000076"
     CDS             complement(10786..11271)
                     /locus_tag="ACFIRM_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC3027822.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(11447..11755)
                     /locus_tag="ACFIRM_000077"
     CDS             complement(11447..11755)
                     /locus_tag="ACFIRM_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027823.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(11739..12041)
                     /locus_tag="ACFIRM_000078"
     CDS             complement(11739..12041)
                     /locus_tag="ACFIRM_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC3027824.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(12115..14478)
                     /gene="pheT"
                     /locus_tag="ACFIRM_000079"
     CDS             complement(12115..14478)
                     /gene="pheT"
                     /locus_tag="ACFIRM_000079"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC3027825.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(14569..15318)
                     /locus_tag="ACFIRM_000080"
     CDS             complement(14569..15318)
                     /locus_tag="ACFIRM_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3027826.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(15324..15605)
                     /locus_tag="ACFIRM_000081"
     CDS             complement(15324..15605)
                     /locus_tag="ACFIRM_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027827.1"
                     /translation="MGLPYQNKDFRNMRLSDYILNNKININNLSDKQVQEIIDYFSSE
                     IDDWLLASEKELKDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(15598..15816)
                     /locus_tag="ACFIRM_000082"
                     /pseudo
     CDS             complement(15598..15816)
                     /locus_tag="ACFIRM_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(15923..16336)
                     /locus_tag="ACFIRM_000083"
     CDS             complement(15923..16336)
                     /locus_tag="ACFIRM_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3027828.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(16353..17342)
                     /gene="pheS"
                     /locus_tag="ACFIRM_000084"
     CDS             complement(16353..17342)
                     /gene="pheS"
                     /locus_tag="ACFIRM_000084"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC3027829.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(17583..17942)
                     /gene="rplT"
                     /locus_tag="ACFIRM_000085"
     CDS             complement(17583..17942)
                     /gene="rplT"
                     /locus_tag="ACFIRM_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC3027830.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(17955..18152)
                     /gene="rpmI"
                     /locus_tag="ACFIRM_000086"
     CDS             complement(17955..18152)
                     /gene="rpmI"
                     /locus_tag="ACFIRM_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC3027831.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(18299..18820)
                     /gene="infC"
                     /locus_tag="ACFIRM_000087"
     CDS             complement(18299..18820)
                     /gene="infC"
                     /locus_tag="ACFIRM_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC3027832.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(18838..20751)
                     /gene="thrS"
                     /locus_tag="ACFIRM_000088"
     CDS             complement(18838..20751)
                     /gene="thrS"
                     /locus_tag="ACFIRM_000088"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC3027833.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGIYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(20804..20880)
                     /locus_tag="ACFIRM_000089"
     tRNA            complement(20804..20880)
                     /locus_tag="ACFIRM_000089"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20844..20846),aa:Val,seq:gac)
     gene            21059..22174
                     /gene="tgt"
                     /locus_tag="ACFIRM_000090"
     CDS             21059..22174
                     /gene="tgt"
                     /locus_tag="ACFIRM_000090"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC3027834.1"
                     /translation="MLKFTLHKKDGLSRRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQAQKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKPDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAVEQGKFADWQAQFHENRARGVD"
     gene            22184..23266
                     /gene="hemH"
                     /locus_tag="ACFIRM_000091"
     CDS             22184..23266
                     /gene="hemH"
                     /locus_tag="ACFIRM_000091"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC3027835.1"
                     /translation="MPSEAASDGILPLYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            23457..23924
                     /locus_tag="ACFIRM_000092"
     CDS             23457..23924
                     /locus_tag="ACFIRM_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFC3027836.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTAFPPLFRSDYIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQIKPF"
     gene            complement(23921..25642)
                     /locus_tag="ACFIRM_000093"
     CDS             complement(23921..25642)
                     /locus_tag="ACFIRM_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC3027837.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAIPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGNIGS
                     MGLKVPR"
     gene            25846..26586
                     /locus_tag="ACFIRM_000094"
                     /pseudo
     CDS             25846..26586
                     /locus_tag="ACFIRM_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            26655..28193
                     /gene="lnt"
                     /locus_tag="ACFIRM_000095"
     CDS             26655..28193
                     /gene="lnt"
                     /locus_tag="ACFIRM_000095"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC3027838.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            28457..29320
                     /gene="rpoH"
                     /locus_tag="ACFIRM_000096"
     CDS             28457..29320
                     /gene="rpoH"
                     /locus_tag="ACFIRM_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC3027839.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(29477..29953)
                     /locus_tag="ACFIRM_000097"
     CDS             complement(29477..29953)
                     /locus_tag="ACFIRM_000097"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC3027840.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            30053..30937
                     /locus_tag="ACFIRM_000098"
     CDS             30053..30937
                     /locus_tag="ACFIRM_000098"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC3027841.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(31028..31543)
                     /locus_tag="ACFIRM_000099"
     CDS             complement(31028..31543)
                     /locus_tag="ACFIRM_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3027842.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(31619..32038)
                     /locus_tag="ACFIRM_000100"
     CDS             complement(31619..32038)
                     /locus_tag="ACFIRM_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027843.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(32159..33157)
                     /gene="holA"
                     /locus_tag="ACFIRM_000101"
     CDS             complement(32159..33157)
                     /gene="holA"
                     /locus_tag="ACFIRM_000101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC3027844.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGNAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(33159..33638)
                     /gene="lptE"
                     /locus_tag="ACFIRM_000102"
     CDS             complement(33159..33638)
                     /gene="lptE"
                     /locus_tag="ACFIRM_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC3027845.1"
                     /translation="MNKIFLTAAALMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAKQIVRRLTFLKAE"
     gene            complement(33690..34469)
                     /gene="pgeF"
                     /locus_tag="ACFIRM_000103"
     CDS             complement(33690..34469)
                     /gene="pgeF"
                     /locus_tag="ACFIRM_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC3027846.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            34922..35896
                     /locus_tag="ACFIRM_000104"
                     /pseudo
     CDS             34922..35896
                     /locus_tag="ACFIRM_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(36023..37147)
                     /gene="rluD"
                     /locus_tag="ACFIRM_000105"
     CDS             complement(36023..37147)
                     /gene="rluD"
                     /locus_tag="ACFIRM_000105"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC3027847.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            37146..37949
                     /locus_tag="ACFIRM_000106"
     CDS             37146..37949
                     /locus_tag="ACFIRM_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC3027848.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(38019..40238)
                     /locus_tag="ACFIRM_000107"
     CDS             complement(38019..40238)
                     /locus_tag="ACFIRM_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC3027849.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VLIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            40625..45226
                     /locus_tag="ACFIRM_000108"
     CDS             40625..45226
                     /locus_tag="ACFIRM_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFC3027850.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQRDNAGTVKG
                     NGEHHWNITSGTNSKIGSTAVRLAGNERDANNGQNVTFEDNGTLVLDQNINQGAGGLF
                     FKGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GQLKVGDGTVILNQQADADKKVQAFSQVGIVSGRGTLVLNSPDQINPNNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTGHASTITLTGKSLITNPNSLSVHSIQNDYDE
                     DNYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQE
                     EAKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVN
                     QSASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKAL
                     NSFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGH
                     YQLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRL
                     YNPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAL
                     RQQAKAEQVKRQQAAEAEKVARQKDEEAKRKAAEIARQQEEARKAAELAAKQKAEAER
                     KARELARQKAEEASHQANAKPKRRRRRAILPRPPAPVFSLDDYDAKDNSESSIGNLAR
                     VIPRMGRELINDYEEIPLEELEDEAEEERRQATQFQPKSRNRRAISSEPSSDEDASES
                     VSTSDKHPQDNTELHEKVETAGLQPRAAQPRTQAAAQADAVSTNTNSALSDAMASTQS
                     ILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDR
                     SLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRS
                     RLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVKVS
                     SMAVKTLTAGLDFAYRFKVGNLTVKPLLSVAYFANYGKGGVNVGGKSFAYKADNQQQY
                     SAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(45302..46504)
                     /gene="trpB"
                     /locus_tag="ACFIRM_000109"
     CDS             complement(45302..46504)
                     /gene="trpB"
                     /locus_tag="ACFIRM_000109"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC3027851.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(46587..47216)
                     /locus_tag="ACFIRM_000110"
     CDS             complement(46587..47216)
                     /locus_tag="ACFIRM_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3027852.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            47492..48271
                     /gene="rsmA"
                     /locus_tag="ACFIRM_000111"
     CDS             47492..48271
                     /gene="rsmA"
                     /locus_tag="ACFIRM_000111"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC3027853.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNNIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            48286..48855
                     /locus_tag="ACFIRM_000112"
     CDS             48286..48855
                     /locus_tag="ACFIRM_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC3027854.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            48966..49421
                     /locus_tag="ACFIRM_000113"
     CDS             48966..49421
                     /locus_tag="ACFIRM_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027855.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            49463..50191
                     /locus_tag="ACFIRM_000114"
     CDS             49463..50191
                     /locus_tag="ACFIRM_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3027856.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            50264..50623
                     /locus_tag="ACFIRM_000115"
     CDS             50264..50623
                     /locus_tag="ACFIRM_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027857.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            50628..51068
                     /locus_tag="ACFIRM_000116"
     CDS             50628..51068
                     /locus_tag="ACFIRM_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC3027858.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            51099..52373
                     /gene="folC"
                     /locus_tag="ACFIRM_000117"
     CDS             51099..52373
                     /gene="folC"
                     /locus_tag="ACFIRM_000117"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC3027859.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALVAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            52387..53427
                     /gene="ftsN"
                     /locus_tag="ACFIRM_000118"
     CDS             52387..53427
                     /gene="ftsN"
                     /locus_tag="ACFIRM_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3027860.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            53420..53917
                     /locus_tag="ACFIRM_000119"
     CDS             53420..53917
                     /locus_tag="ACFIRM_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC3027861.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            54111..55661
                     /gene="purF"
                     /locus_tag="ACFIRM_000120"
     CDS             54111..55661
                     /gene="purF"
                     /locus_tag="ACFIRM_000120"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC3027862.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     ISDAGDEE"
     gene            55761..56252
                     /gene="greB"
                     /locus_tag="ACFIRM_000121"
     CDS             55761..56252
                     /gene="greB"
                     /locus_tag="ACFIRM_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC3027863.1"
                     /translation="MSIETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            56311..56937
                     /locus_tag="ACFIRM_000122"
     CDS             56311..56937
                     /locus_tag="ACFIRM_000122"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC3027864.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT        14438 a        15923 c        13663 g        12956 t
ORIGIN      
        1 ttttgaattg gcacgcccac ggggaatcga accccggtta ccgccgtgaa agggcgatgt
       61 cctaaccgct agacgatggg cgcatattgt attttcttac tggcgcaccc ggagcgattc
      121 gaacgcccga ccctctggtt cgtagccaga tactctatcc aactgagcta cgggtgcggc
      181 acaatcactt gttcaagtga ttaagaaagc gtgataatac ggatttatct gagtcctgtc
      241 tattatttta gacttctatt ttcagttatt tttgtaactg attgatttta aaataaacta
      301 tttttcgacg caagttgcag caattctgcc gcatgttgcc gcgtcgtatc ggtaataact
      361 tctccgccca acatacgggc aacctcttcg atccgttgta tttcatccaa tatactgatt
      421 tcgctgacgg tttgctctcc ctcgctgtgc ttgcgcaccc gccagtggtt ttctccgcag
      481 gatgcgactt gcggaagatg ggtaacggca agcacctgat gttttctgcc caacgcacgt
      541 aatgccttgc cgaccatttc agccactccc cctccaatac cggtatcgac ctcatcaaaa
      601 atcagggtgg gaacttgggt atattggctg gcaacaacct gtaaggcaag gctgatacgc
      661 gccaattcgc cgccggaggc gactttgttc agcgggcggg gcggattgcc tttgttagcg
      721 gcaacttgaa attgaacctg ctccaaaccg tgtgccgtcg gcgacgaagg caacaggacg
      781 atgtcgaaac gcgcgccttt catggcaagg tgttgcatat gctcggtcgt ttcgctactc
      841 aaacgccctg ccgcctgatg gcgcatggca gaaaggatgt gggcagcttc ctgatattcg
      901 gcaaaattgt gggcaacatt atgctcgagc gcgtccaaat cagcggcagc ttgaagactt
      961 tgcaggcgtt cttcgatttc tgccaacttg gcaggcaact cttcgggttc aatccggtat
     1021 ttccgcgcca tccccatcag ttcgcccata cgctgttcct gtgccgccaa ttcattgggg
     1081 ttgatgtcac tgtgtcctgc cacatcgcgc atattggcac tgatttcgcc caattcggcc
     1141 tcgatgcttg ccaacatatt caggctctcg gcaaagcgcg gctcgatgtt ttgcagattg
     1201 gccaatagtt tttgacattg atagatatgg cgttggatgc cgttgtcgcc gtcaatcttg
     1261 cttccgactt cttcggcggc ctgcaacaat tcggcagaat gggcaaggct gtcgtggctt
     1321 tggctgagag cttcccattc gccttgttta atgtccaact gattcagttc gttaaactgc
     1381 cattccagac gctcccgctc gataatgacg gcatcggcgt gttcctgcgc ctcttggagg
     1441 gcttttttcg cattggccca gttttgataa agctgcctga cggtttccgc ctgcaccctg
     1501 ccgcccacaa atgcgtccaa caattcgcgc tgggcagctt cctgattaag cgaatgatgg
     1561 tcgttttgcc cgtggatgtc gataagctgc ccgccgacgg ctttgagttg cgccaaggta
     1621 gccgcctgat tgttgataaa actgcggctt ttgcctttgg catcgataat gcggcggata
     1681 ctgagttctt ccccgccctc atccaacagc ccttgttcac gcaattctgc ttttaaagca
     1741 ggcaaatggg aaatatcgaa caatgccgac aactgcgctt cttttgcgcc gctgcggact
     1801 tggctgtaat cggccttatc gcccaacagc agaccaatcg catccaaagt aatggacttg
     1861 cccgcgccgg tctcgccggt caaaacggta aaaccgcttt gaaaatccag attcagattt
     1921 tcgacaatga caaaatcacg caaagaaagt gttagaagca ttgtgaggat tccttagcaa
     1981 ttctaccgac aaaagcaata tcaactttag atatacgaat tatatctaag attttttaaa
     2041 ttaaaaataa gtttttcctt aattttgctt agtgtttgtt tttatctgct tatttgttgc
     2101 aatgccgtct gaagcaatgt gcgtttcaga cggcatttgg aatttcagtt gggcagggta
     2161 tcggacggta cggtttccgg ctcttcttca tccgtaggcg gcatttctat caaatcgggc
     2221 aaaaccagtt cgcccagttc ggtcagcggc ggcagttctt ccaaaccgtc caagcgcaaa
     2281 tcgctgagaa aagttgccgt tgttgcccac aatgcgggtt ttcccaatgt gtcccgatgt
     2341 ccgatgactt caatccaccc ccgatcctgc aaggtctgca tcacgttctg cgacaccgcc
     2401 acgccgcgta taccttcgat gtcgccgcgc gttacgggct gctggtaggc gataatcgcc
     2461 agtgtttcca tcacggcgcg ggagtagcgc ggcgcacgct gttcttgcag gctgcccagc
     2521 cgctcgaatg ccgtctgaac aatctgaaaa cgccagccct cctgcgtatg caccagttgc
     2581 aacgccctat cctgccaacg cgttttcaac tgcgccaaca catcaatcag tttgtcttgc
     2641 gacaacggcg gcacacacag ttcgcgcata gatttttcgg tcagcggttc ggtttgggtc
     2701 agcagtgcgg cttcaatcag cgcgtcggga gaaattttgt cggtcatacg ggtatccgtg
     2761 ctgaaacggc atgggtttgg atatgccgtc tgaaatcggt tggagtagag agaagctgcc
     2821 tgaaaaatat ttttcagacg gcattcttta tgcttccgaa ggcttctgcc ctttgcgctc
     2881 tgcttttctg gcttcgcgaa tggctttgag ttcggcttct ttctgacgtg cttttttaag
     2941 ccaagtttcc cattggttcg gtgtttcaag ggtgattctg ccgattttgc cttcgcggaa
     3001 gtccgtgagg atgttttcgg cggctttttg gtagttgacc cgtccgccgc tgaggactgc
     3061 gccgcgtttt ttggctatcc attcgagcca cgagttgtcg tcccagtggc tgctggggtc
     3121 tttgtcggct tggtagcgtt cttgcagcat agggaggtag tggcggcgga ggtagtctaa
     3181 aagttcgagg gcgacttctt cttcgtccaa tgcgttgcgt ccgactgcgc cgccggcggc
     3241 gaggttgtag ccgctttctt cgacgatgat tttcggccac agcattcccg gggtgtcgta
     3301 cagccagaag tcgtcggcaa ggaaaaggcg ttgttcggct ttggtaatgc cgggttcgtt
     3361 gccggttttg gcggattttt tgcctatcat tccattgatg agggtggatt tgccgacgtt
     3421 gggaatgccg caaatcaaaa cgcgcagggg tttgtctatg ccttgccggt ggggaatcat
     3481 cgcgcggcag gcttgggtga ttttgccgtg tgcgccggtt tcggaggaat cgagggcgat
     3541 ggcccgggtg tcggggcggc tgttgtagtg gtcaagccag acctttgtgc gttcagggtc
     3601 ggcaaggtct tgcttgttta aaattttgag tttgggctta cctttggaaa gctgcgccaa
     3661 cagtggattt tcgctggagg cgggcatacg cgcgtccagc atttcaatca ccatatcaac
     3721 gcttttggca cgctcggcga tggcttttct tgccttgttc atatggccgg gaaaccattg
     3781 gattgccatg tctgttcttt cttctcaata tttgaaatgc cgtctgaaac ggcggacggg
     3841 gtttcagacg gcataatggt ttgacgggat tagcggcccg acaggttttg cgcctgcctg
     3901 tgccgtatca gcaattcata acgccctttc aaccttcggg cgagtttttc gacaatatag
     3961 accgaacgat gctgcccgcc ggtacagccg atggcgacgg taacgtagct cctgctttca
     4021 tcctccaaac gcggcaacca acgcgtaaca aacctttcga tgccgtcaac catttcctgc
     4081 gccaacggct gtccgtccaa ataatcccaa acgggcttgt ccataccggt gtaaggcctc
     4141 aactcgggat cgtaatacgg gttgggcaga ctgcgcatat cgaacataaa atcggcgttg
     4201 ttcggcacac cgtatttgaa cccgaaggac tccaaaacca ccagcagtcc ggtacgctca
     4261 accttcagcc actgccggac tgcatggcgg agctgttggg cattcatctt ggaagtgtcg
     4321 atacagtagg cgatttcctt aagcgggaac agccattcgc gctctttttt caggctttcc
     4381 aacagcgtca tatcctgatt gctcaaagga tgtccgcgcc gggtttcgga aaagcggcgg
     4441 accagcacgg cctcttccgc ctcgacaaac aaaacttcca ccctgtgtcc gagtccgcgc
     4501 agataggcaa tctgctcgcg cgcttgggca atgtctatgc cggaacgcac atcgacgctg
     4561 accgccaatt cggtttcgtc cgcacgttcg atatggtacg acaccagcga cggcagcatt
     4621 tccaagggca ggttgtccac gcaaaaatag cccaaatctt ccatttggcg cagtgcaacg
     4681 gatttgcccg agccggaaag cccgctaatc aggacgattt tcatgattct gttcgttttc
     4741 tttaagttgc gtctgatggc gttccaaaaa ttcgcgcgta ctgtctttgc cgcgcaactg
     4801 caaaatgtaa ttgcgtaccg ccgcctcaac caaaacggca aggttgcgtc cgacggcgac
     4861 gggcagcgta accgaacgga cgttgacgtt gaggatggat tcggtttcgg tgcggatgct
     4921 caaccggtcg agctgcttca tatactcgtc gtccgcctcg accaaattga taatgagttg
     4981 caggattttt ttggggcgga tggaagtttc gccgaaaata tggcggatat tgagtatccc
     5041 caagccgcgc acttccaaaa aatcgcgcag cataggcgaa caacgccctt ccaatgtttc
     5101 cggaccgatg cggaacagct cgaccgcatc gtcggcaatc aggctatggc cgcgcgaaat
     5161 cagttccaat gccaattcgc tcttacccaa accagaatgt ccggtaatca gcacgccgat
     5221 ttcaaacaca tcgagaaata cgccgtgttt gacggacgat gctgccaagg tgcgttgcag
     5281 gtaaatccgc aacacatcca tcagataagg gctttcgagt ttggaagtca acagggggat
     5341 gtcgtttttg tggcagtagt cgcgcagccc cggggaaacc ggcaaatcgt ttgccacaat
     5401 gaccaaagac atggaaatat cgaacaggtc tccgaactga taacccgtct cccccgattc
     5461 gaggcggttc agatattccg actccgccaa accgaccact tggatttggt tgggatggat
     5521 gaaattcagg tgtccgacca gggcgaggac gggcttgtcc gcctctacgc cgatacggtt
     5581 gtccgcaccc gaattgccgg cggcccaggc aagttgcaac ttgtattggt tgtcgtcaaa
     5641 caggcggcgg acggagatac tgggcatatt ttattcttcg gtcaggatgg cgcgggcttc
     5701 ttccgcagaa gcaaccgtca tcagcgattc tctgatgctt ttttgggaaa acttgccggc
     5761 cagtttggat aagacttcca aatgctcgcc cgttgcgttt tccggcacca gcaagataaa
     5821 aaccagtgaa accggtttgc cgtcaggcgc gtcgaatccg acgggttcgc gcgtacggat
     5881 gaacgcgccc gtcgcctgct tcacgctcgc gtggcggccg tgggggatgg cgacaccctg
     5941 ccccaaaccg gtcgaaccca gtttttcacg ggcaaaaaga cattcgaaaa catcagcatg
     6001 ggacaatgag gattcgcgtt ccaaaagcag gcccgcttcc tcaaacagcc tttttttact
     6061 gcccacctcc atatccaaaa caatatggga caaaggcaaa atttcgccga taaggctcat
     6121 aagcttctct tttcagacat cgcaaaacag aaggattgta ccgactgccg gggcaaacct
     6181 caatcccgca tacggtacgg gctgacataa cacagcgttt caaaaacata ttttaacgct
     6241 tttcggcaca gatagaaatg ccgtccaaag cagtttacgg ttcttcagac ggcattgccc
     6301 tgccttattt cgcgaaaaac gtatgggcaa aaatagcggc aaaccacacc caaccgattc
     6361 tgttgtttgc caaaaacgtt tcaaaacaga tttgccggac gcggcttttg attgcggcat
     6421 attggcggta ttgcagcagc aggacgatgg ggattgccgt ccaatatgcc catgccgcac
     6481 cgataaccgc acccaatact gccatcagca gggtaaagcc gccatgacac agcataacgg
     6541 cggcgatgtc gtaacgcccg aacgtgacgg cggaggtttt gatgccgatt ttcaaatcgt
     6601 cctctttgtc cgccattgca tagacggtat cgtaagccag tgtccataaa acattggcgg
     6661 caaagagtat ccacgcttca acgggaacgc tgttgcccac tgcggcaaac gccatcggga
     6721 tgccgaagga aaaggcaaat cccagataaa attggggaat cggaaaaaag cgtttggtaa
     6781 acgggtaagt cagcgcaagg aacagcgcgg gcaggctcat cagccatgtc agatgattca
     6841 gcggaatcag gcacaatgcg gcaagcagac acaaaaatgc cgtcagcagc agggcttctt
     6901 ttttcttgac cctgccctgt gcgaacggac ggtttttggt gcgctcgaca gcaccgtcaa
     6961 aatcgcggtc ggcaaagtcg ttgatgacgc agccggcact gcgcattaaa aacgtgccga
     7021 ttgtaaacgc cgccaatacc gccaaatcgg gaatgccgtc tgaagccagc cacaatgccc
     7081 agtaggtcgg ccacagtaaa agcagcgtcc caatgggctt gtccgccctc atcagtctga
     7141 tataaacgtc caaacgatcg gacagtcgta aaaataaagg ggatttaaga ttcataaccc
     7201 tctttcatat aagtcttaca cccttggaaa actaatccat aaacttctag ttccaataac
     7261 caatttgaat tattgtcgta aaatcttatt atttttagac catcttcttg gttctttatt
     7321 tctacttggc atatatttct attgttatgc gaccaatttt gacatttcat tcctttaata
     7381 aagctatttc ttaattcaat ttcaactgta tcataatctt ttagccacag cccccatttt
     7441 tcgatttcta cagaaggctt tgtacttgtt cggatagtta aaatacaatt agacaagtca
     7501 gttgaaaaag agactagtga aatatccatt atgccactag gatatatttg ttttagaaaa
     7561 cgattcctac ttattatttc tattatattc atatttctta ctaaaaatta tagtgtccgt
     7621 gagtaccatc gacatgtcct gtatctaaat tggagtctct tatattaaaa tgtggtttag
     7681 atgcaggttg ccgcacagct tgaaaaaacg gtattttatc cgataaagcg tttcagttcg
     7741 ggcagaaaat actcggtcag cagcatttcc tcgccttgat gggaaaaccg agaacgccgc
     7801 gcggcaaagt accgtccgca tccttcactg gaaacggcaa actcaaacgc cgaacgcgcc
     7861 ccttccaaat cggcttgaaa cagacgctcg cccaaagggc gtgtgccgca gtccaaaatg
     7921 ttttgccaaa acgccgaacc gacgctgcac gcgcttcgtg cctgcacaac agcagtgccg
     7981 tccagtttca gaagcacatc gcgcaccctc cgccgtccgt attccgtttc cgcttcgccc
     8041 agtttcagca gttccaccga aaatgtatgc ggcaaggcgc acaatgcggc ggtcagcgac
     8101 cgggctttca ataaccggct catcggcagg tcgatgccgt ctgaaacggg ggcgggcaag
     8161 tcgggcagcc atttcccaaa taggtgttcc atatctttcc caatctttat accgcgcctg
     8221 tttttgccaa ctccatccat tccgcttcgg gaaaatcggc tttcagacgg cattgcagat
     8281 agttcaggct gtcttgggcg acgtgtgcgt cgcggctgtc gtggatgtgg ttaaacacga
     8341 ggctgtgcag gcgaatgccg tattgtttga gcacgacgaa actgagcagg gtgtgattaa
     8401 tgcttcccag ccgtccgctg gtaacaagga tgacgggata agcctgttgc tgaatatgat
     8461 caatggttaa cagcttttcc gtcagtggta ccatcaaccc gcccgcgcct tcgaccaaaa
     8521 cgacttcgta ctgcgccgcc aattcttgtg tggcggtgcg gattttgtcc aagtccaaac
     8581 ccctgccgtc cagtcgggcg gcaaggtggg gcgaggcggg ataactgaag atttcaggca
     8641 tagtcagacg ctgttcatca gcttcctgca tgggtatgcc catgatttta cggtggacgg
     8701 cgatgtcttc ggcgatgtct tggcaaccgg tttgcacggg cttttgcgta atcacgcttt
     8761 tgccctgctg caacaattgt tttgccaaca taccggtggc gacggttttg ccgatgtccg
     8821 tgtctatacc gctgacgaag taaacgcctt tcatttgctg tgttccttca agatttgcac
     8881 ggttttatcg gcaagtttgg tcaaaatgcc gtctgaaatg atatagggcg gcatcagata
     8941 caccagcctg ccgaacgggc gtacccaaat gccctgcgcc acgcaatccg cttgaaaacg
     9001 cgccatatcc acgccttttt ccaactctat cacgccgata gcacccaaaa cgcgcacgtc
     9061 tttcacgccg cgaatgtccc acgcggcttt cagacggcct tttaagatgc tttcgatacg
     9121 gcggatattt gcctgccagt cttgggataa aagcagcttg accgaagcac aggcaacggc
     9181 acacgccagc gggttcgcca taaatgtcgg gccgtgcata aacacgcccg cttcgccgcg
     9241 tgaaatcgtt tcggtaactt tttgcgaagt gattgccgcc gccagcgtca tatagccgcc
     9301 gctcaaaccc ttgccgatgc acataatatc cggcacgacc tccgcgtgtt cgcaggcaaa
     9361 catcttgccc gtgcgcccga atccggtggc gatttcgtca aaaatcaaca cgatatcaaa
     9421 ttcgtcgcac aaatcgcgca agccgcgaag atactgcgga tgataaaaat acatgccgcc
     9481 cgcgccttgc acgaccggtt ctaaaataaa ggcggcaata tccacatgat gcgcttcaaa
     9541 caaggcgcgg acaggctgca aatccgcccc gtcccattca tcgtcgaaac ggtttttcgg
     9601 attatcgaca aaataacgct gcggcaacgc gctgccgaaa atatggtgca tccccgtttc
     9661 cggatcacaa acggacatcg cgttccaagt atcgccgtga tacccgcgcc gtaccgtcgc
     9721 aatattctgc ttcgccgtca gaccccgcgc ctgctggtat tgcaccgcca ttttcaacgc
     9781 gacttctacc gaaaccgaac ccgaatccgc ataaaaaata cggtccagcc cctgcggcaa
     9841 aatcccgacc aacaacttgc ccagctccac cgccggctcg tgcgtcaggc cgccgaacat
     9901 cacgtgcgcc atttgtttca tctgattctc aaccgcctga ttcaaaacgg gatgattgta
     9961 accgtgtatc gcacaccacc aagacgacat cccgtcaatc agccgcgtgc cgtccgccaa
    10021 ttcgataaac accccttctg cacgtttgac aggataaacg ggaagcggat cggtcatgga
    10081 agtataggga tggagcagat gggtacggtc gaaattaagt aatgatgatg tgtgttgatg
    10141 ttcagacggc ataagtttct ctcttttctt attactgcat tcaaacgcaa aaagcgtatt
    10201 ctactccgac agaccgcttc ccacaccttt ccatccgttt cgggcgaaaa accgcgaaac
    10261 aaatcgtccg caacataagc gcacaccgtt tcgcattcct caagcctgat tggaatcaga
    10321 cgacccaacg cccaataccg tttcacacag cctccgccca acgcttcaat caatcataga
    10381 taaatacgat acagatcata gcaacagcca tcgcaacagc gttggcaaaa tcaggggact
    10441 ccggcatagg cgcatagcat ctgccgatgc acggctcctc attcggctct atgaatacca
    10501 tacccatcac aaaatccact gctaaaacca ggcacggctt cttatactta tgatagattt
    10561 ccaccatcct gtcccatata tcccaaacac tcataccgta tatcccgcag gcaacaaatt
    10621 ccgattgaag gttacagccc tattttatag tggattaaat ttatagtgga ttaacaaaaa
    10681 ccagtacggc gttgcctcgc cttgccgtac tggtttttgt taatccacta tattttcaaa
    10741 ccggaaaaat gccgtctgaa ccttcagacg gcattccatt catatttatt cgtctttttt
    10801 gtgttcgatg gcgtttcggg aacgattgcc gccgtaaggc tcttcaaccg tatattctcc
    10861 ctcgataata tcgtcatcgt ggaaaaatcc ctcttttctg cccgattggt tcatgttgaa
    10921 aaaattttcc gcacctcctg cctgcaacac tgcccctccc ttaaacggca gcagcagcaa
    10981 taccgccaac accgaggata cgaatcccgg actcatcaga cacaccgccg ccaccgtata
    11041 acggataggc cacaacatct gataaacaga taccttccca ctacttttta ccgccgcgcc
    11101 agccaataaa agaccggaca gccccgtatg cctgagcatc agcacaccgg cggcaaaggt
    11161 tgccgccatt agaaacagcg tccaaccgcc gcccagccaa tcggcaaccc acacaatcga
    11221 cataatttcc aaaaacagca gcaccaaaaa accgataccg aaaaatctca ttgaccgtca
    11281 tccttatatt taagtaaaca gcaaaccgcc cgaacaggat tccaagcgag ctgcctgtaa
    11341 atgattacaa aaccatgtgc ttcaagccga aacaatgtga aatcacgcaa taattatttt
    11401 tagcgtgtaa aacaaaaccg gctgcatacc tgcaaccggt ctcaaatcag cacaactcct
    11461 tatccaaatc ctccaacagg tctttcagac cgtctccgat ttcctgaagc gtaaccggac
    11521 ggttgccgtc cgaacccgat tccgcacctt ctgcatacgg tgcaaacgtg ctcttcagtt
    11581 tcaacagttt caccacatcc aaacgggtgt agcgttctcc gccgtaaccg acaaccacac
    11641 cgtgatcatg ctgccattgc gcaaaaccat aggggctgat ttgcaacagt ccgcacaact
    11701 cgtccagcgt gaaatagcgt tttgcaggaa tcgtcaaatc aacgttgttt gtcatagtag
    11761 tgttccacca tgcctttgag tttctggctg gcatggaaag ttaccacgcg gcgggcggta
    11821 atcggcactt cctcgccggt tttcggatta cggcccgggc gttgcggctt gtcgcgcaac
    11881 tggaaatttc cgaaaccgga aattttgatt tcttcgccgc ttgccaaagt gctgcggatt
    11941 tcttcaaaaa agagttcgac aatttctttg gcatcgtttt tggtgacgtt gctgacttta
    12001 tccaccaaaa tatcggccag ttctgcttta gttagtgtca tatgattacc ttctgttgta
    12061 acagttgtgg attattacca atttttattt aaaattcaag cgaatattta ttttttaact
    12121 gcgaagccgc gcccctgcct ccgttgccac gccaatcagt tttccaataa gcggctcgac
    12181 cgcctcatcc gtcagcgtgt tttccatatc ctgcaaaatc actttaaccg ccacgctctt
    12241 catcccttcg ggcagacccg tgccgcgata cacgtcaaac acgctgattt cctgtaccaa
    12301 cttgtttgct gcgcctttca agacaagcag taaatcatca tgattcataa cttccggcat
    12361 cacaaacgcc aaatcgcggc gcaccggctg gaatttcgat acggcccgat agcgcgtttt
    12421 cccgcattcc aacacagccg ccatatcgat ttcaaatacc agcggcgctt gcggcaggtc
    12481 gtatttttgc agccatttcg gatgcagttc gccgacaaag ccgatgactt tgccgtctga
    12541 aacgatattg gcggcacgtc cgggatgcag ggcgggatgt ccggttttaa cgaactcgac
    12601 cgctttgttt ttcaacagat tttccacgtc cgccttgatg tcgtaaaaat ccgcattgcg
    12661 cgttttctca ccccattgtt cgggcatcgc cgcgccgtac cacagaccgc cgatgcgttc
    12721 gttttggaca aaccggccgt ctgaaccttt gccgaacacg caggcgattt caaacacgcg
    12781 cacgcggttt tgcttgcggt tcaggttgtt ttgcaaaatt tccactaagc cgccaatcaa
    12841 cgtagaacgc atcacggcat attgcgccgc cagcgggttt tgcaggcgga tgggggcggc
    12901 gttggcggca aaatcgagtt cccactgctc gtcaacgaag gcatagctga tcacttcgcg
    12961 gtagccgcgc gccgccattt cgttgtaaac ggcaaaacgc ggacggcgtg tttcgggcag
    13021 ttccgacatt ttcagacggc ctgacgtgta atcgtcgggg atgttttcat agccgtaaac
    13081 gcgtccgatt tcttcaatca aatcagcctc aatttcgatg tcgaaacgga agctcggcgc
    13141 ggtaatgcgg aagccttccg ccgttttctc aggctgcaga cccaagtgtt gcaaaatggt
    13201 ttccacctgt tcggcaggaa tgtccacgcc caacacggtt ttcagacgac ccaaacgcaa
    13261 tccgacctgc ttggcttcgg gcaatttacc ttgtgcttcc accatctcgc ctgccgcacc
    13321 gccgcaaatc tgcaaaacca attcggtggc gcgttcaatg gcatccgcct gcaaacggta
    13381 atccacgccg cgctcgaagc ggaacgacga atccgaaccg aaaccgtatt ggcgcgattt
    13441 gccgacgatg atttcgggct caaaccaagc cgcttccagc acgatatttt gcgtgccgtc
    13501 tgaaaccgcg ctcgccgcgc cacccatcaa gccggccaag ctcaacgcac ctttttcatc
    13561 agcgaccacc agtgtattgt cagccaaagt aaccgtcttc tcattcagac acgccaaagt
    13621 ctcgccattt tgagcacggc ggacaatcaa actgcccgac agcttatcag catcgaaaac
    13681 atgcataggc tgaccgattt ccagcatcac atagttgcca atgtccacca atgcggaaat
    13741 gctgcggata ccgctgcgtt ccaaacgttg tttcatccaa tcaggagtag ccgctttcgc
    13801 gttgacattt tcaataacgc ggctgataaa acggccgcag tcagccggtg catcaatacg
    13861 gacagcctgt tttttctcac tgccgatagg ggccgtctga atttcaacag gcgtaaacgc
    13921 acattgagtc aacgcagaaa cctcacgcgc aatgccctta acactcaagc aatcagcgcg
    13981 gttaggcgta attttcaacg taaacagcat atcgtccaaa tccaagtatt cgcggatgtt
    14041 ggtaccgaca ggcgcatctt caggcagaat gtgcaggccg tctacaccat catcaggcaa
    14101 acccagttca ttggtcgaac acagcatacc gtttgatggt acgccgcgca ttttagtcgg
    14161 cttaatttta aaattgcccg gcaaaacggc aaccggcaac gaacacggca ctttaatgcc
    14221 cggcttgaca ttcggcgcac cacaaacaat ctgaaccaac tcgcccgtac cggcatcaac
    14281 ttgagtaacg ttcaaacggt ctgcatcagg atgtttttca acagatttta cttcggcaac
    14341 aaccacgcca ctgaaagcag gggcggcagt atcgatttct tccacttcca aaccggccat
    14401 ggtcaaaaga tgttccagtt tatcggcaga aagatcagga ttggcttggg ttttcagcca
    14461 tgagtaggag aattgcatgt tatgttctca atcaattaat taacttttgt tttatcaatt
    14521 ttcgttcaga cagtctgttt tagaattctg gttaatgttg atatttactc aactctgaaa
    14581 aatattttct ctgtgaaatt ttaaaaattc cagacttggg tttagtcttt caggcagtct
    14641 tatcatccga ttttcatatg gtttaaataa atcatcaaat ccttctgttt ttgccagtag
    14701 tggagaaaat ataattttga aattttcatc tattcctaac aaccccctat caaaagcttt
    14761 gtcatgtaac gcattcaaac atagcccatt ccttgggttt aaacggttat ccttatcttc
    14821 cccccagggt ttgatatgac tagcaactaa caattcaggt tgttttaatc cagtaataca
    14881 acattgatta ttgtatgctg ccaaaacact ggaacggaaa aagccctgat tgacgcgtac
    14941 tgatattctt gcaaaccttt cccttccctt tggaatctca ggtgattcta tttcaatatt
    15001 actattctca tcatttgatt ggtattggga aatgactccc tcaaattctt tattaagttg
    15061 ctcccaatct cctgaaaact cattccatac gacaacatcc attttactgc cattacttaa
    15121 tcccgaaata tttttgactt tcagcgtagg atctaaacga cccaaatttc ctatcttcat
    15181 accgagtgcg gacggtgtcc tgcccagtat ttcagcatat tcttgaataa caggattgtc
    15241 cttactaacc ttttgaaaag gaatgatgta ataaagataa agggcaacca gggtttcatc
    15301 tctagtccat tttttcattt ttatcagcta tctttctgat tttccataag gaaatcgtac
    15361 aatacagatt ttcttattcc ctgaacatat tgctgtccat ctagtactaa tttgtatagg
    15421 tatacatcat aatcttttaa ctccttctcg cttgccaata accagtcgtc tatttctgaa
    15481 gaaaaatagt caataatttc ttgtacctgt ttatcagata aattatttat atttatttta
    15541 ttattcaaaa tataatcaga aagcctcata tttctaaaat ctttattttg ataaggtaaa
    15601 cccacacagc acatcaaatt gcttcggaat gtattgtttg atttcttcca aagtaatatc
    15661 gccaaacggc acttctccga aattggcttc gtacgtcagt ttggcttttt cgtcccactc
    15721 gctcgaaaac acgtattccc cgcccaagtt ctgcatcgcg atgcggaagc ccccgatacc
    15781 tgcgaacaag tcgataaagg taaatttggg tttcaaggcc gtctgaaaag tatcgttgtt
    15841 ttcaaaaagg cggtattgtg ctgctttttc ttttaaccgt tcatcctgcc catcttcctt
    15901 aatcttgttt ttgtaaatca attcatcttg tttggatgat ttctttgact ttttcggcac
    15961 agcaggttgt atttttgacg acatcgttac tccaaaaacg taaaaccgtc cagccctcgg
    16021 ctttcagacg gcctgttact tctatatccc tttggatatt gcgctcaatt ttggcaatcc
    16081 aaaactcacg atttcccttt attaccgctt ttttctgttc ccaatcctta ccgtgccaga
    16141 attccccatc gacaaatacg gcaactttat attttttaaa tgaaaaatca ggttttccaa
    16201 aaatgcttcc gctatttttc cgatacctga gccccaaagc ccacattgcc ttcgccaaca
    16261 caagctcggg tttcgtccct gtacttttgt tggactgcat acattttttg cgctgctccg
    16321 gggttaattt atccattggg tttatttgag atttattcaa actgcttcaa gaagttcaaa
    16381 tcgttatcga agaacaagcg caggtcattg acgttgtagc gcaacatggc aaagcggtcg
    16441 agaccaatac cgaaagcgaa accggtatat ttttcagggt cgatattgac atttttcaac
    16501 acattaggat gtaccatacc gcaaccgcct acttccaacc atttgccgtt ttctcccatg
    16561 atgtcgattt cggcagaagg ctcggtaaat gggaagaaag acggacggaa acgcacttgt
    16621 aggtcatcgc gttcaaagaa gcgacggata aagtcagtga acactgcttt taagtcggca
    16681 aaagttacgc cctcttctac ccacaaaccc tcggcctgat ggaacatagg cgagtgcgtg
    16741 gcatcgctgt ccacacggta aacgcggccg ggggcgataa tgcggatggg cggctctttt
    16801 ttatcgagca tatagcggat ttgaatcggg gaagtgtgcg tacgcaaaac atcgccgttt
    16861 tcaacgtaaa acgtatcctg catcgcacgg gcaggatggt ttgcagggat gttcagggct
    16921 tggaaattgt gaaaatcgtc ttcgatttca ggcccgtccg ccacttcgaa acccattccg
    16981 tgaaagagtt cgaccacacg ttgcaaggtc agggttacgg gatgcaggct gccgccttcc
    17041 tgagcgcgtc cgggcagggt aatgtcgagg gcttcggcgg caagtcgggc ttgcagcttg
    17101 gcttcgttga gggcatcgcg tttggcatta aaagccgtct gaaaccggtt tttgcattca
    17161 ttgatatgcg cacctatggt tttgcgctct tcaggcgaca tttgccccaa agttttcaga
    17221 agtccggtca actcgccggt tttgccaaga taacgggctt tgatttgttc tagagcgttg
    17281 aagtcttgcg cagcttctac tgcggcaatg ccttctgcaa cgatacggtt tacattttcc
    17341 ataatatcaa gcctgtcaat taatgatatg ccacctgaaa caacgaggcc gcctgtatag
    17401 cctgacatat cagactcttc agacgacctt attttcttca cggccaatcc attggataaa
    17461 ttttctgtat tttaacgcaa atttatttaa aaaaatagac tttaaagaaa gaaaatcaat
    17521 cacatagaat ttccgcaaac aaagaaaaaa ggaagccgca gcttcctttt caattttttg
    17581 gattaagcag ccaaagcagc tttggctttt tcaaccaatt gtgcaaaagc ggctttatcg
    17641 aacacggcca aatcagccaa tactttgcgg tcgatttcga tagaggcgcg tttcagaccg
    17701 ttcataaatt tgctgtaaga caacccgttt tcacgcgcac ctgcattgat acgaacaatc
    17761 cacaattgac ggaattggcg tttgcgttgg cggcggtcac ggtatgcgta ttgaccagct
    17821 ttcattaccg cctgcttggc aacgcggtaa acgtttttac gacgaccacg atagcctttg
    17881 gctaacgcga agattttttg gtgacgggca cgggcggtaa caccgcgttt tacgcgtggc
    17941 atattctaaa ctccttaagc gtagggtaac attttagcaa cagaagccaa atcgcgatca
    18001 tttaccatag aggtaccgcg cagttggcgt ttgtttttgg tggtcttttt agtcaagatg
    18061 tggcgtttga acgcatgagc gcgtttcaca ccgccgttac ccagtacttt aaagcgtttt
    18121 ttcgcgctag acttggtttt cattttaggc atgggaaaac tccattcgtt atcggataag
    18181 gcattagggg gttttaaaac atcggtttca aacacttgaa cccacaatgc cacggttttt
    18241 tgccgcaatt gaaaacttac tccgaagcgg caatcggagt aagcggaaaa ttatagcttt
    18301 attttttctt cggcgcaatc atcatcacca tttgacggcc ttccattttg ggaaaggact
    18361 cgatttgcgc cacttcagcc aaatcttctt ttacacgttc caaaagttgc gcgccgagtt
    18421 gctggtgagc catttcacgg ccgcggaaac gcaatgtcac tttgacttta tcgccgtcgg
    18481 caaggaagcg gttgatgttg cgcatcttga tttgataatc gccctcatct gtacccggac
    18541 ggaatttgat ttccttgatt tgcacctgct tttggttttt cttggcttcg tcgcgcttct
    18601 tggcttgttg gtatttgtat ttaccgtaat ccatcagttt gcacacagga ggtttagcag
    18661 ttggggaaat ctctaccaaa tcgacatcct gcccttcggc catagccaaa gcttcacgaa
    18721 ctgaaacgac accaagctgt tcgcctgact cactgattaa acgcacttct ttggcggtaa
    18781 tttcaccgtt gattcgtgct tcgcgttctt gagcgatgat gaatactcct ataaaaatta
    18841 atgattgacg agggcatcag tgatttcttg ctgcaattgc gcaatgaaat catccaaatc
    18901 caaagaaccc aagtcttccg ctttgcggcg taccgcaact ttgttttctt gtttctcttt
    18961 atcgccgata acgatttgat aagggaaacg gtattggctg ttgtcgcgga ttttgtaacc
    19021 gattttttca ttacgcaaat ccaactcggc gcggaagcct gccgcctgca atttggcagc
    19081 aacttcccga caataatctg cctgattttc ggtgatgttc ataatcacca tttgaaccgg
    19141 tgcaagccat aacgggaaag agcctgcatg gttctcaatc agaatgccga caaaccgctc
    19201 caaagaacct aaaatggcgc gatgcaacat aacaggccgc gctcggtcgt tgttttctgt
    19261 tacatattct gcatccaagc gttccggcaa gacgaaatcc agttgtaatg taccgcattg
    19321 ccaagaacga cccaaggcat ctttgacgtg atattcgatt ttaggcccgt aaaacgcacc
    19381 ctcgcccggc aattcgcccc attccacgcc gcaggcagtc aatgcctcgc gcaaaccctg
    19441 ctctgcctta tcccacacgt cgtctgaacc tgcgcgtttt tcagggcgaa gggaaagcct
    19501 gacggataca tcatggaaac cgaactgttt gtagatgcga accaacaatt cattgaacgc
    19561 acgagcctcg ctgacgattt gatcttcggt acaaaaaata tgcgcatcat cctgcacaaa
    19621 accgcgaacc ctcatcagac cgtgcagtgc gccgctcggc tcgttgcggt ggcaagaacc
    19681 gaattccgcc aaacgcatcg gcaaatctcg atatgaacgc aaaccgttgt tgaaaatttg
    19741 aacatgaccc ggacagttca tcggtttaac cgcatattcg cgtttttccg aactggttac
    19801 gaacatattg tctttgtagt tgtcccagtg gccggatttt tcccaaaagg ttttatccat
    19861 aatttgaggc gttttgacct ctttataacc ggcggcgttc agctctttac gcatatgctg
    19921 ctcaatcact tgccacaaag cccagccttt aggatgccaa aacaccatac ccggcgcttc
    19981 gtcctgcaag tggaacaaat ccagttgctt gcccaatttg cggtggtcac gtttttctgc
    20041 ttcttcaatg cgttggatat agtcttttaa ttcgtctttc gtcgcccaag cagtgccata
    20101 aatacgttgc agcatttcat tattgctgtc gccgcgccaa tatgcgcctg ccagcttggt
    20161 cagcttgaag tttttcagga agcgggtgtt cggaacgtgc ggaccgcggc acatatcgac
    20221 atattcctgg tgatgatata tccccatcgc ttccacttcg ggcatatcgt caatcaggcg
    20281 cagtttgtat tcttcgccgc gctcttgaaa aattttaatc gcctccgcac gcggagtcat
    20341 gattttgacc acatcataat cttgggcaat caattctttc atacgcgctt caatggcggc
    20401 aacatcttcc ggtgtaaacg gtttttccgt ggcgatgtcg taataaaagc cctcttcaat
    20461 gacggggccg ataaccattt ttgcattagg atagagttgc ttgacggcat gcccgacaag
    20521 atgtgcgcag gaatggcgga tgatttcgat gccttcctga tctttcggag taatgatttg
    20581 aacagcagaa tcctcaacaa tcgggtcgca cgcatcgacc aatttgccgt ttaccttgcc
    20641 tgccaccgcc gccttcgcca aaccggcacc gatagacgca gcaatttgag ccacggtaac
    20701 gggggattcg tattggcgga ctgaacagtc cggcaaggta atattcaaca tcaaacgctc
    20761 cgaaagatta aaaaataacg gcataaatgc cgttatggga atttggtagg cacgattgga
    20821 ttcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctacgtgcct
    20881 tcaaagaatt tttgtatttt atcgggtcgt tttaattttt gcaagagaga tgtgcatttc
    20941 tttatgcaac attgtggcaa gatttgggga taccgtctga aaaaccgtgg gaattcattt
    21001 ataatgccgt cttttaaaat atatttcgac ccctgtcgcc caacttacat tcaatcttat
    21061 gcttaaattt actttacaca aaaaagacgg tctctcccga cgcggcacgc tggagttgaa
    21121 ccacggaaaa atcgaaacgc cggttttcat gccggtcggt acttacggtt cggtcaaagc
    21181 gatgaacccg caaaacctgc acgacatcaa ggcgcaaatt attttgggca acacttatca
    21241 tttgtggctg cgtccgggtt tggaggtcgt cgaacagttt ggagggcttc acggttttat
    21301 cggctgggac aaaccgattc tgaccgactc gggcggtttt caggtttttt ctttgtcgga
    21361 tatgcgcaag ctgaccgaag aaggctgtac gtttaaaagc ccgattaacg gcgacaagct
    21421 gtttttatca cctgaaattt ccatgaaaat ccaaacggtt ttgaactcgg atatcgcgat
    21481 gcagttggac gaatgcacgc cgggcgaaac aacgcgcgaa caggcgcaaa aatccctgca
    21541 aatgagcttg cgctgggcgg aacggagcaa aaaagccttt gaagatttga aaaacccgaa
    21601 cgcgctgttc ggcatcgtgc aaggcgcgat gtatgaggat ttgcgcgaag aatctttgcg
    21661 cggtttggag gaatttgatt ttcccggcct tgccgtcggc ggtttgtccg tcggcgaacc
    21721 caagcccgaa atgtaccgta tgctgcacgc cgtcggcccg atgctgcccg aacacaaacc
    21781 gcattacctg atgggcgtag gcacgccgga agacctcgtg tacggcgtgg cgcacggcat
    21841 cgatatgttc gactgcgtga tgcccacccg caatgcacgc aacggctggc tgtttacccg
    21901 tttcggcgat ttgaaaatca aaaacgccaa acacaagccc gacaagcgtc cgatagacga
    21961 aagctgcacc tgctacgcct gccaaaattt cagccgcgcc tacctgcacc atctgcaccg
    22021 cgccggcgaa atcttgggcg cgcaattgaa caccatccac aacctgcatt tctaccaagt
    22081 catcatggcg gaaatgcgcg atgccgtcga acaaggcaaa tttgccgact ggcaggcgca
    22141 attccacgaa aaccgcgcgc gcggcgtgga ctgattcccg gcaatgccgt ctgaagccgc
    22201 ttcagacggc attttgccgc tttaccctac ctgcactatt tttcagaaac cgatcatgct
    22261 tccattcctt ccagaaccct ccctttccta tacgcaacaa aaccgtaccg ccgttttgct
    22321 gttgaacctc ggcacacccg acgcaccgac cgcacaggcg gtacgccctt atctgaaatc
    22381 ctttctgacc gaccggcgca tcgtcgaact gcccaaatgg ctgtggtatc ccattctgca
    22441 tggcctggta ctgacgttcc gccccaaaaa aagcgcgcac gcctatgaaa aaatctggtt
    22501 taaggaaggt tcgcccctag aagtttacac agcacgccaa gctgccgcgc ttgccgaacg
    22561 tatgcccgac ctcatcgtcc gccacgccat gacttacggc aatccttcga tagcggatgt
    22621 attggcggag ctgaaatccc aaggtgtagg cagattgctt gcgattcccc tatatccgca
    22681 atatgccgct tccagcagcg gagcggcggt agataaagtg tgcgaacaac tgctgctgca
    22741 acgcaaccaa atgagcgtcc gcaccatttc gcgcttttat gatgatgccg gctacattga
    22801 tgcaatgaaa aaccacatcc tccgatattg ggcggaacac gggcgtggga aaaaactgat
    22861 gttgagtttt cacggcgtac cgcagaagca ctacgacctc ggcgacccct atcccgacga
    22921 gtgccgccac accgccaaac tgcttgccga agcactggaa ctgaccgaag acgaatatac
    22981 cgtatcgttc caaagccaat tcggcagggc aaaatgggtt acaccgagca cgcaggattt
    23041 gttcggcaaa ctgcccaaac agggggtaac cgagctggac gtattctgcc cgggcttttt
    23101 ggcagactgc ttggaaacca tggaagaaat cgccctgatg gggcgggaac agttttatga
    23161 agcgggcgga aaaaactacc gctacatccc ctgcctcaac gacaaccccg actggataga
    23221 cgcactcgtc gcacttgccg aagaaaacct tggcggctgg cgttgaaact gtttgagcca
    23281 tgtctaaaat gccgtctgaa cgccgtgttc agacggcatt ttcccgctac tttagcgtat
    23341 gatggcatct atgccggaca gcacatcaag tgcaacctta ctaccgattc ggcaaaaaaa
    23401 cctttcaccc gctacgcaaa atcaacaaca aggtttcaaa cattaaggaa acgccaatga
    23461 acacaacccg actgccgacc gccttcatct tgtgctgcct ctgcgccgcc gcttctgccg
    23521 ccgacaacag catcatgaca aaagggcaaa aagtgtacga atccaactgc atcgcctgcc
    23581 acggcaagaa aggggaaggg cgcggcactg cgtttcctcc gcttttccgg tcggactata
    23641 ttatgaacaa accgcacgtc ctgctgcaca gcatggtcaa aggcatcaac ggtacaatca
    23701 aagtcaacgg caaaacctac aacggattca tgcccgcaac cgccatcagc gatgcggaca
    23761 ttgccgccgt cgccacttat atcatgaacg cctttgacaa cggcggcgga agcgttaccg
    23821 aaaaagacgt aaaacaggca aaaggcaaaa aaaaccaaac agacaaaatg ccgtctgaaa
    23881 ccggcaatcc ggcttcagac ggcattcaaa tcaaaccttt ctaacgcggt actttcagcc
    23941 ccatagaccc gatattgccg ccaccgtcaa cctccttcgc ggtcagcata aagccgccga
    24001 tgtcaatacg gtcgccctcg accggctcct gactgtcgga acgcttatcg aaaagctcgg
    24061 caaggctcaa accctcttcg cccgcttcca gcttcaaacc gtaagcaagt gccaaatcgc
    24121 ccgaacgtgc tgccggcgaa acgacaaact caccgaagaa atcaaaattt tcacggacgc
    24181 cgatacccgt ctcggtaaag tatttcgcca ttttatcgac cttgccgtcg ggcaggatgt
    24241 accacgccaa atcctccgcc tgaagcacgg tgtcggactg catttcgatt cggctgccgt
    24301 tgcgtatcaa tgcgaaacag cgtgcgtcaa acgaatcgga aatcggttcg accgtatcgg
    24361 gatggtgtcc ctccgcttca gattcggcaa ccactttaaa cgccgacatt ctgacaattt
    24421 ccttttccgc cagccaaata tcatgcgtat ctttcggttc gggcttgttg ggcatggcaa
    24481 ccttcaacag ccgcgccatc acaggaatcg tcgttccctg aatcagcagc gacagcacca
    24541 ccacggcaaa cgccacatca aacagcaggt gcgaattggg aacgcccatc accagcggca
    24601 tcatcgccag cgaaatcggt atggctcctc gcaagcccaa ccaactgata tacgcctttt
    24661 cacgcaggct gtaattgaat ttccacaaac cgccgaacac tgccagcgga cgcgcgacca
    24721 gcatcaggaa cgccgcaatc gccaaggctt ccgccgccct gtccaacacg ccggcgggag
    24781 aaaccagcag accgagcatg acgaacaaag ttgcctgcgc cagccaagcc aaaccgtcca
    24841 tcacacgcaa aacgtgttcc gtcgcacggt tgcgctggtt accgacaatg atgccggcaa
    24901 ggtaaaccgc caaaaagccg ctgccgccta tggtattggt aaacgcaaac acaagcagcc
    24961 cgcccgacac aatcatcagc gcgtacagac cttccgcaag attcagacgg cgtaccagct
    25021 ttgccaatat ctttccgccc gcccaacccg tcagcagacc gaaaccgatt tgcagcgcaa
    25081 gcatccggac aaacgctgcc gcacccgatt ccgccggctg cataatcatg gtaatcagtg
    25141 ccgtaaccaa aaaaaccgcc atcgggtcgt tcgcacccga ttcgatttcc aaagtcgcct
    25201 gcacccgttc gttcaaacgc acgccgctgt tgcgcaaaag gctgaatacc gcgccggcat
    25261 cggtcgaacc gacaatcgcc gccatcagca cgccgaactt ccaatccaaa ccgagataaa
    25321 aagttgcaaa cagtcccagc ggaagcacag tggcaaacac gccccacgaa gcgagtaccg
    25381 acgcgggctt caacgcaatc cgaaaactgg aaagctgcgt ccgcaaaccg ccgtcgagca
    25441 gaataaccgc caaagcaagc tggctgatga aattcgccat cacgacatta ttgaaggcaa
    25501 tgccgccaac gccttcctcg cccgccagca tgccgacggc aagaaacacc agcagcaaag
    25561 gcattcccaa acgtgcggac aaagtggtcg aaaccacgct caggaacaac agtgcgccac
    25621 ccagcagaaa caggctgttc atccaatcca cgccaacggc tcccccttaa aattcatgaa
    25681 attagaatcg aaagattgta acacggcatt ttcttctcac gaaactttat ttcatgtccg
    25741 ccaccgcctt cagacggcat tttcctgttt catgccgcct gataccgccc aaacccgcgt
    25801 tctcctatat aatgtcggac tttgcgccgc atttcaggat tttttatggc atttgcctca
    25861 ctatttactc tgccggacta catcgccgcc gttttggacg atgttgccct gatgaccaaa
    25921 gccgccgcaa agaaaaccgc cggcgtggcc ggcgacgatt tggcacccaa tgccaaccgg
    25981 gttacaggcg tatcggcaga acgcgagttg ccgattgttt ggccggtggc gaaaggttct
    26041 tttgccaaca agccggtttt ggtgtctgcg gctctgctgc tttccgcctt cctgccgcaa
    26101 ctcattacgc cgcttttgac ggcaggcggg atttatttgt gctttgaggg cgtggaaaag
    26161 ctgctgcaca agtttctgca ccgacatgac gcgcacgatg acggcggcga aacggcggtt
    26221 aacgagcagg ctaaaatcaa aggtgcaatc cgtaccgact ttatcctgtc cgcagaaatc
    26281 atcatcatcg cgctgtgcgt ggtcgaacaa tacagcctga tgacgcgctc gctcgtgatg
    26341 gcggcaatcg gcatcggcat gactgttttg gtgtacggaa tcgtcgccgt catcgtcaaa
    26401 ctcgacgact tgggcatgct gctgatgcgc cgcccacaac ctcggtcttt tgcacgattt
    26461 cctgcacgcg caacactgga acgctggctg gacagagtat tccgccaacc tcgttgtcgg
    26521 gccgctttcc ggttcgattg cctgcgcccc gccttgtcgc tgatgaatcg tttcggcagg
    26581 cattgattcc tttttcaaat accgatgccg tctgaaagac gttcagacgg catattccga
    26641 acagacagac gaatatgttt tccaaactgg acaaatactg gcagcacccc gccctctact
    26701 ggcctttgct catccttttt gccgccgcca cccccttcgc cttcgcaccc tactaccact
    26761 tttggctgat gcccttgatt ttcggcgcct tcgtccgcct catcgaactg cgtccgcgtt
    26821 ttgccgcctc ttccgcctac ctgttcggtc tgaccgcata cacggcacag ttctactgga
    26881 tacacactgc cctgcacgac gtttccggcc tgcccgacct ctatgccgta ccgctgacct
    26941 tcctgctgcc cgcctacctt gccctttatc cggcactgtg tttctggctg tggaaaaaat
    27001 ttaccctgcc ccagggcata aagaccggtc tggtactgcc catcctgtgg acgctgaccg
    27061 agtttgcccg cgagcgtttc ctgaccggat tcggctgggg cgcaatcggc tactcccaaa
    27121 tcaccccgga cagcccgctg gcgggtttcg cccccttggg cggcatccac atggttacac
    27181 tggcaaccgc ctttctcggt gtctggctgg ttttggcgat cgacaacacc gcacgttcgg
    27241 gcaaacgcct gcttccggcc attctgattg ccgccctgct tgccgcaggc tacaccgccc
    27301 aacaaaccga cttcacccgc cccgacggca gccgcagcac cgtcgccctg cttcagggca
    27361 acatcgccca aaccctcaaa tggcgtgaag accaagtcat cccgaccata cagaaatatt
    27421 acgaacaggt cggcaaaacc actgccgaca tcgtcatcct gcccgaaacc gccattccca
    27481 tcatgcgcca aaacctgccg gaaaacatac tggcgcaatt cgccgaacag gcgcaaaaca
    27541 acggcagcgc gctcgccgtc ggcatcagcc aatacacttc ggacggcaac ggttacgaaa
    27601 acgccgtcat caacctgacc ggctatcagg aaaacaatca ggacggcatc ccttactacg
    27661 ccaaaaacca cctcgtcccc ttcggcgaat acaaaccgct gcccctcctg accacaccgc
    27721 tttacaaaat gatggacatg cccctttccg acttccgcaa aggcggcggc aagcaatccg
    27781 ccatgatgat gaaaaaccaa aaaatcgcct tcaacatctg ctacgaagac ggattcggcg
    27841 acgaactgat tgccgccgcc aaagacgcca cactgcttgc caatgtcagc aatatggcgt
    27901 ggtatggaaa atccaacgcc atgtaccagc acctccaaca atcgcaggcg cgggctatgg
    27961 aactcggacg ctatatggtc cgcgccacca acaccggcgc aaccgccatc atctccccca
    28021 aaggcaacat catcgcccaa gcccaacccg atacggaaac cgtattagaa ggacacatca
    28081 aaggctacat cggcgaaacg ccctatatga aaaccggcag ttcatggtgg ttgatgggca
    28141 tattgaccct agccgcactg attcttttca tcttccgaaa caaagaacac tgatacctgc
    28201 acagtccgtc cggacgggct gtcttttttc aaaccggccg tttacataca accacgtatg
    28261 taatataatt ctaaaatcga attgagctat caggtttaca aaatcaatca aacttgcctt
    28321 acaaaatttt cctgtctttc aaaccttagc gtatatctcc tgccaaacat acaaaataca
    28381 agccaagcag tgatatactg catcccaaca caccaaaccg ccgatacgca gtttcagccg
    28441 aaaggaaatg ccccaaatga ataacgcttt cgcattaccc gccatccaaa gcggcaacgg
    28501 cagcctcgaa caatacatcc acaccgtcaa cagcatcccg atgctgtccc aggaagaaga
    28561 aacccgcctc gccgaacgcc ggataaaggg cgacctcaac gccgccaaac aactcatcct
    28621 gtcccacctg cgcgtcgtcg tttccatcgc gcgcggctat gacggctacg ggctgaatca
    28681 ggccgacctg attcaggaag gaaatatcgg tctgatgaag gcggtcaaac gctacgagcc
    28741 cggtcgaggt gcacgcctgt tttcatttgc cgtgcattgg attaaagcgg aaattcacga
    28801 attcatcctg aggaactggc gccttgtccg cgttgccacc accaaaccgc aacgcaagct
    28861 gtttttcaac ctgcgcagca tgcgtaaaaa ccttaatgtc ctgtctccca aagaggcaca
    28921 agacatcgcc gacgatttgg gtgtcaaact ttccgaagtt ctggaaatgg aacagcgcat
    28981 gacgggacac gacatcgcca tcatggcaga caacagcgac gacgaggaca gtttcgcccc
    29041 catcgactgg cttgccgacc acgattccga accgagccga caactatcga aacaggcgca
    29101 ttacgccctg caaaccgaag gcctgcaaaa cgccttggcg caattggacg acaggagccg
    29161 ccgcattgtg gaaagccgct ggcttcaaga cgacggcgga ctgacgctgc accagcttgc
    29221 cgccgaatac ggcgtatctg ccgagcgcat ccgccagatt gaagcgaaag ccatgcagaa
    29281 actgcgcggt ttcctgaccg aagaagccga agcggtttga aatatacaca ataaaatacc
    29341 cgacaaaaca atttgtcggg tattttattt ggtaaacatc aatgcggttt gtgcgtcgtg
    29401 ccgtacacga ttttatccgc ccaagccatc gcaatcgcca aaatcaaaaa gcagacatgc
    29461 accagacact gccacatcag ctgcttttcc gacagattgg cggaattgat aaatgtttgg
    29521 agcaaatgga tggatgaata ccgataatcg acatcgacag ccttaccttc aataccggtg
    29581 cattcacatg gctcaaccac tcgggccggt caggatggtc gtcgatacgc aaccgggata
    29641 cgaacgactc atacccgcca atctgcacca tggtcagcaa attcgcaatc ataaccacat
    29701 caatcagatt taaaacagcg agcatgatgg cgttttcgtc cgacacatcc aaattcatga
    29761 ccagatgctt caacgatttc aaaaacttat aggcacaaat cgcccgtaca acagtcagcc
    29821 ccgcataaat cggcacttgc agccaacggc ttgcaaaaat cattttggca aaaaacccat
    29881 agcctgttcg ggttttggag attttgcttg atacatttcc cttccttgca gcaacaaaag
    29941 cgcgtgattc caatctaccg ctgcgaaatc ttcaattttt cacacccgaa aacgcttgcc
    30001 gccatacaga tgtgatatat tgattccatt ttaacgaact tcggagaacg acatgagcag
    30061 cggaaatatc cgcatccaca gcatgaccgg ctttgccaac gcggcggcag agtgcggcag
    30121 caaacgcatc aacctcgaca tccgcgccgt caaccaccgc tttttggaca ttcaaatcag
    30181 gatgcccgac gatttgcgcc atttggaaaa cggcatccgc gagcagattt catcccatct
    30241 ggcgcgcggc aaagtcgaat gcaaaatcca aattcaagat gcggaaaacg gcaatcagtc
    30301 tttggaactg aatcgcgttc tggtcggaca gcttgccgaa atcaacaaaa acctccgcaa
    30361 acattatgat ttggcaaaac tgggcgttgc tgatattttg cgctttccgg gcgtattggc
    30421 aagccaaaag gaaaatacgg aagcattggc gaaaaccatt gtcgaactgg ccgacaaagc
    30481 actaaaagac tttaccgccg cacgtcggcg tgaagggaaa aaattgggcg aacacctgtt
    30541 acaacgcatt aaaaacatgg aagaaatcat cgatgctttg agcgaaatct tccccgcgct
    30601 ggtggaagcg cataaagaaa aaatccgcac ccgccttgcc gaagccgtcg gcagcatcga
    30661 caacgacagg ctgcaacagg aatttgccct ctttatccaa aaatccgaca tcgacgaaga
    30721 attcagccgc ctgcgcaccc acattgccga agtgcgccgt atcgttaccg aacacaaagg
    30781 cagcagcggc aaacggttgg acttcctgat gcaggaactg aaccgcgaag ccaacacttt
    30841 gggcagcaaa tccatcgcta ccgaatgcac ccaagcctcg gtcgagctga aagtcctgat
    30901 cgaacagatg cgcgaacagg tgcagaatat cgaataaccc tgcctttctc tccaaacatg
    30961 acaatgccgt ctgaaacttt tttcagacgg cattcttgat cggctgaaaa cctctgttgc
    31021 aagcaattca agattgttct ttcacatcca ccaacctcgt tccggcaagg aagtcataca
    31081 gaaactgccg gtcgggattc agcagggcga acccccacgg caaaatcagc caaaccaatg
    31141 ccgcgccggc cgcgcccttg ggcggaatgc cgaggaagtg gcgcaatccg gcataagcaa
    31201 gcataggaat gaagacgatg aatatgcacg cccaaataaa gcgcaggcgc agcaggtgca
    31261 aagacggctg tatgccgtta aggtcgcaca agccgatttt ccatgtcctc atcgccaagg
    31321 tctgcccctg accgtgccag ttggcgcgga aataaagcca ccatgctccc attatcagga
    31381 tactcgttac caatgcagaa accgcgatag aaacgggatt cagaaaaatg gcggcaatac
    31441 cggcaagcaa tgctgccaaa caggtcgccg caccgaccag cagcatctca tacagtatgg
    31501 cggcaaagcg gcgtttgagc ggggcggttt tcagtatcat catcttttca atcggaatat
    31561 gccgtctgaa acgccaacag acttcagacg gcatttgtgc aaagatgcct tgcggcggtt
    31621 aacggtgcgg acgtttctga ccgcgcgact gttttgccgc catttgattg atttcctgac
    31681 gtgtcgggcg ttttttgaac atagcgtgcg cctcgtccac cgaacatttt tgcgacaggc
    31741 agatcagggt aatcgcatct ttctggtgtt gtccgccgct gtggtacata cggcgcaggt
    31801 ttgcctcgtt gattctgcct ttttcgggat cgatgtcgaa ttttttcaga cgttcgataa
    31861 actggcgttg ctgtcggcgg gatgtccaaa caaagccgcc cgcagcggcg gcgacgatga
    31921 cggcggcaag caggatggca agcggccagg aatcgacaat caatacgata atgaggctta
    31981 aaacggcggt ggtcagtaaa atcaggccgc ccaagcgcag tttggtctta ttgtccatag
    32041 ggttgatgtt cctttattga ggtggtcgtg ccgtctgaaa tgttttcgga cggattggtg
    32101 aatacttttc aggattaacg gtattttaca gtattttcgg attttgggga ttaccgcttt
    32161 attccgccag cgacacgaca agctgtttga ataccgtcca tgcgtcgccg tcttccgcgc
    32221 ctttgatgat tcggtcgatt tgggcgcagg ttttgagcgc gtcaagcagg cggacgacgg
    32281 aaatccgctt gaccgcaagc ggtgcgagcg tctgtttgtc gccccaaagc ctgaggctgt
    32341 tgcggacgga ttggatgctc tgcccctgtt tcagggcagc ggcaagccga atcagcgtcc
    32401 gcacgtcttc ggcaaccgcc cacagaagca gcaccggttc ttcgccttct tcctccaacc
    32461 cgtccaacag cctgcatacg cgcgggacat cgcccttcat ccacgcgcct gccagttgga
    32521 acgcgtcgaa gcgggcaaca ttggcaacgg cggtttgcgc ctcatcgata ttgacggcat
    32581 gccctttcgg atacagcagc gcgagcttgt cgatttcctg acgtgccgcc aacagattgc
    32641 cttccacgcg ctcggcaaac agtgccaatg cgtctgcctc gatacccaaa ccgattttgt
    32701 ccagccgtcc gcgtatccat tggggcaaag ccgccgcacc gaccggtttg gcttcccaca
    32761 cttccccctt tgccgccaat gcggcaaacc atttggactg gagccgggtt ttctccagtt
    32821 tgggcagcaa aaccagcgtt accgtatctt ccggcagtcg ggcggcaaaa tcctgcaatg
    32881 cctcgccgcc gtttttgccg ggttttccgt tggggatatg caattccaac agtttcaaat
    32941 cggcaaacag acccgcattg cctgcggttt gcagcagttc gttccaatcg aaagacgcgt
    33001 cggcggtata ggcttcgcgg ttgagataac cctgcttctt cgccgccgcc ctcaatgcgt
    33061 ccactgcctc gatacgcagc agttcttcct cgccgtggat gacgtacaga ggtttcaaag
    33121 gcgcgtccgt atcgatgcgt ccgatatgtg ccgccacgtc attccgcctt cagaaaggtc
    33181 aggcggcgga caatctgttt ggcaacatcc tgccgcattt ccgcccacag ggtttcttct
    33241 tcttcctgtt tgcccaaaat ttcgttgtcg gcataatcca aaatgcggcg gacggacacg
    33301 gtcatcggtt tgccgaccgg ctcgccgcgt ttcaatacct gcgcttcaac cgtcaatatc
    33361 aaaagatatt cgttgatgac tgccgcacgg gtaacggtat aggtttcctt gttttgggaa
    33421 acgctgtcta tacgcagggt catctgcgcg ccggcagcat cgtccaccct gcccgaagcc
    33481 tgatacagcg cggtttccaa aggaaactgc aatgcctgtc cgccttcgat gtgccagctc
    33541 cggtaggtca gcggcggaga aatgccgtct gcacctttca ggtggaaacc gcacgcgccc
    33601 agcatcaagg ctgcggcagt aaggaatatt ttgttcatcg cttgcctttc tcaaaagcat
    33661 aaagtcggta gattatatca gcggcgtgtt cagacggcat tgccgtccag ccaaatcagg
    33721 ctcgccatac gccccgtcgc tccgtcgcgg cggtaggaaa agaaaatatc ccgttccaaa
    33781 accgtacaat gcgtgccgcc gtacacgccg cccacgcctt cgcgcctcag aatcaggcgg
    33841 gcgagcgcgt agaggtcggc aaggaatttg ccgccgccga tgggttcaaa tgcagcagcg
    33901 gcttcgggca tggacgtgca gaacgcatca aacacgtcct gcccgacttc aaacgcatcc
    33961 gcactgatgg cagggccgag atacgccata atttcgacag gcggaacctt catcgcggca
    34021 atggtgtttt gcaatacgcc gcccgccaaa ccgctccagc ccgcgtgtgc ggcggcaacc
    34081 gccgtacctg ccctgtcgca aaatagaacg ggcagacagt ctgcagtcat cacggcacag
    34141 acggctttac ccgtgtcgtc cacggaagcg tcggcatcgg gcgtgccgtc caatgcttcg
    34201 gcagcattga cgacaatggt gctgtggatt tgattgagat aggcaacggg caatccgacc
    34261 tgctgttgca cgatttcgcg gttgcggcgc acggtttcag ggtcgtcgcc gacgtgcgta
    34321 ccgaggttca aactctgata cgcaccttgg ctcacgccgc cgttgcgcgt ggtaatcagg
    34381 gttttcacat tggcgggcgc gggccaatcg gcggtcagga agtttttgcc tttcggggcg
    34441 agatttaggg tttctgtgat ggttttcatt ggatattgga ataggtgtgc ggcggaaagg
    34501 gttgattaac tgccttcggg gcaaatggat gcttgttttt tcgtcattcc cgcacaggcg
    34561 ggaattcgga gattcgggat tgttttcaaa cgttcgggtc ggattcttgc cggtgcggga
    34621 atgatgccct tatatacttt ctccgagctt tatatgcttc aacaggggac ggcacatcaa
    34681 gcaccgcact gcgtgttgcc cgaacgaggc tgccctccga ttagattcta tcgctataaa
    34741 cagacgggtt tcaaccgaaa aggaatgggg ataaagtcca tttccaacac ctctcgggcg
    34801 atgccgtctg aaaaccaatc tccactttca gacggcattg tttaggcgcg gtcattttgc
    34861 aaattaaggt taaggcgtgt atgcttgcct tcccttaaaa acaaacacgc aacgcattat
    34921 tatgaatatc ctcagtaaaa tcagcagctt tatcggaaaa acattttccc tctgaacctc
    34981 gctctttgcc gccgccgctt ttttcgcgcc cgacaccttc aaatgggcgg ggccttatat
    35041 tccttggctg ttgggcatta ttatgttcgg tatgggtttg acgctcaaac cttccgactt
    35101 cgatattttg ttcaaacatc ccaaagccgt catcatcggc gtaatcgcac aattcgccat
    35161 tatgccggca accgcctggc tgccgtccaa actgttgaac ctgcctgccg aaatcgcggt
    35221 cggcgtgatt ttggtcggct gctgcccggg cggtacggct tccaatgtga tgacctatct
    35281 ggcgcgcggc aatgtggctt tgtcggttgc cgttacgtct gtttccaccc tgatttcccc
    35341 attgctgact cccgccatct tcctgatgct tgccggtgaa atgctggaaa tccaagcggc
    35401 cggtatgttg atgtccatcg tcaaaatggt tttgctcccc attgttttgg gtttgattgt
    35461 ccataaggtt ttgggcagta aaaccgaaaa gccgaccgat gcgctgccgc tggtttccgt
    35521 tgccgccatc gtgctgatta tcggcgcggt agtcggggca agcaaaggca agattatgga
    35581 aagcggcctg ctgatttttg cggttgtcgc actccgcaac ggcatcggct acctgctcgg
    35641 cttctttgcc gccaaatgga ccggcctgcc ttatgatgca caaaaaacgc tggccatcga
    35701 agtcggtatg caaaactcgg gcctggccgc cgcgcttgcc gccgcacact ttgccgccgc
    35761 gccggtcgtt gccgttccgg gcgcattgtt cagcgtgtgg cacaatatct ccggctcgct
    35821 gctggcaact tattgggcgg ccaaagccgg taaacataaa aaaaccctaa acgcctttca
    35881 tcataatgcc gtctgaacgg ggcaaacgaa accggcggtt tctgcccggc cccttcagac
    35941 ggcctttgtt tgaaatcggc ggggacgatt gcccggctgc tccggttcgg ctgcctgcgt
    36001 tcgccccgcc tttcaagccg cctcactccc ttacataaac cacttcgaca tcgtagtcgt
    36061 cttcgttcca atcgtcatcg tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc
    36121 tcaaagacga atccaaaccg gcttcaagac ggaggacgga caacaaatga tatatgtcgt
    36181 ccggaatcgg tgcctcaaac gaaacggttt cgccgctttc cggatgggta aaactcaagc
    36241 ggtaggcgtg caacgcctga cgcgcgccca aacttttaac ggcttctttc accgtgtcgc
    36301 cgcacggatg gcgcgggttg ccgtaaaccg ggtcgccggc aagcggatgg ttggcctcgc
    36361 gcatatggac gcggatttgg tgcgtcctgc ccgttccgag cgagcattcg atgtagctgt
    36421 gggcaagata gcgttccaac actttgacgt gggttacggc aggcttgccg ccgaatttga
    36481 cggctgccat tttcaggcgg ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt
    36541 cgaaggggac gatgccgttg gcgacggcgc ggtagatgcg tttgaccgtg cgttcttgaa
    36601 gctgcctcac gagggaattt tgcgccggca gggttttggc aaccaccatc agcccgctgg
    36661 tttctttgtc caaacggtgt acgatgcccg cgcgcggaat ttgggacagc tcggggcaat
    36721 gcgccaacag gccgttgagc agcgtccccg tccagttgcc cgccgccgga tgcaccacca
    36781 gtccggccgg tttgttgacg acgatgacgg tatcgtcttc gtaaacaata tccaaatcca
    36841 taggctccgg aacaaacgcc agattttcct cactcggacg gacggttaca caaatttgct
    36901 cgccgcctat cattttgtct ttgggttgcg aaggtttatc gtttacaata accgcgcctt
    36961 ctttaatcca tgatgtcagg cggctgcgcg aatagtcggg cagaagtttc gccaacaccg
    37021 catccaaccg cccgccggca agctcgagcg gaacggtcag accgatacgg ttttcagttc
    37081 cggaagctga cgcaaagtct gaatcgtcgc tataatcgga ttcattatca aaggaagtat
    37141 tctgcatgaa aaaaattctt ttaacggttt cattaggttt ggcactgagt gcctgtgcca
    37201 ctcaaggtac ggccgacaaa gatgcccaga ttacccaaga ttggagtgtg gagaagctct
    37261 atgccgaagc ccaggacgaa ttgaacagca gcaattatac gcgggctgtc aagttatacg
    37321 aaatcttgga atcgcgcttc cccaccagcc gccatgcccg gcaatcccaa ctggataccg
    37381 catacgccta ttataaagac gatgaaaaag acaaggctct ggcggcaatc gaacgcttcc
    37441 gccgcctcca tccgcagcat ccgaacatgg attacgcgct gtacctgcgc ggcttggtgc
    37501 tgtttaacga agatcagtcc ttcctgaaca aactggcctc gcaagactgg tccgaccgcg
    37561 acccgaaagc caaccgcgaa gcgtaccagg cgtttgcgga actcgtccaa cgcttcccga
    37621 acagcaaata cgccgccgat gcaaccgcac gcatggtcaa actggtcgat gctttgggcg
    37681 gcaatgaaat gtcggtggcg cgttactaca tgaaacgcgg cgcatatatc gccgccgcca
    37741 accgcgccaa aaaaattatc ggcagctatc aaaatacacg ctatgtcgaa gaatcactgg
    37801 ccatattgga acttgcctac aagaagttgg acaagcccca gcttgccgcc gatacgcgcc
    37861 gcgtattgga aaccaacttc ccgaaaagcc cgtttttgac gcacgcttgg cagcccgacg
    37921 atatgccctg gtggcgttac tggcattaag cccatcctcg aaatgccgtc tgaagccctt
    37981 tcagacggca ttttccgttc cggcaccagc cttcccgttc actcaaacgg ttttttctgc
    38041 caatagaatt tatacactct caaacgttga gcctttacgc ctccgcgtcc caagccgaat
    38101 tgcagcgcgc ccgacaaatc cgtccgcaac aacttgatgc cgtgtgcgcg gacacggttc
    38161 tgcaccgctt cggtcggatg cttgtaggcg ttggcataac cgcttgaagc aacggcatat
    38221 tcgggcgaaa cggcattgag gaacacgccc gacgaggacg tattgctgcc gtgatgcccc
    38281 aacaccaaca cctggctgta caggttgcct ccatacttgc cgaccaggct ttcctcgccc
    38341 ttcgtatcca aatcgcccgt taccagcagt gccgcaccgc ccgccacaac acgcaaaaca
    38401 caacttttcc cattatcatc gatatttttg cgttcagacg gcctcaaaaa ctcgaaatcc
    38461 acgccgtccc attgccaacg ctgttccgca caatgccgcg ccccctcata aaattccggc
    38521 tgtccggcat aaatcccgcc gttcggtatc ttgccgactg cctgaaagcc gccgtcgtgg
    38581 tcgctgtcgt gatgcgacag aaccagcttg tccaaacggc ggacacccgc cgcattcaaa
    38641 ctcggcacga tacccgtttg tgccgccgct accgttcccg tatcgaaaag cagatggcgg
    38701 tttgccgtcc gcacaagcac cgacaagccc tgccccgcat cccaaaccgt aaccgcagct
    38761 tcgttttcag gtactgcttc aggacggtag gatacaaacc ctgccaacag caacaccgcc
    38821 cacggacgca aacccaagcc gcgcggcagc agcaacagca aagcggcaca caccgccaac
    38881 accagcagcg gcaaaggtgc ggcggcaacg gcaaactcag gcgacacatc ggcaagccac
    38941 accaaaaacc gcaaagtgta ttccgccaaa aatgcccccg cctgctgaag cggcgcaaac
    39001 ggcacgaccg aacccagcaa cgccagcggg gtcaacaccc aagaaaacca cggtatcaac
    39061 accgcattga ccaaagggct gaccaaaggc agcgaggcaa acagataacc gagcaaaacc
    39121 agagacaaca ccgaagccgc ccactgcccg cgcacggcgg tttgccgttt cccctcgtac
    39181 aaacgccctg cacacgccca tatcagggcc gccaccaaac cgaaagacag ccaagtaccg
    39241 acacccaaaa ccgccaaagg gtcgaacagc agcaccgccg ccaacgcctg ccaccacgtc
    39301 gcccacgccg acagtcttcc cctgcgccac gcccacgcga acgccgccaa catcaaaaca
    39361 ctgcgctgcg tcggcacgga aaaaccggca agcagcgcgt aaaacagcgc gcccgcacac
    39421 cccgccgcca aaacccacgc gcgcggccgg gcgggaaggc gcggggaaca ggcaagcagc
    39481 cgcttcgcca gccacgcaaa cagcaccgcc accatcgtaa cgtgcaaacc cgagatactg
    39541 accaaatgcg tcagccccaa cggccggaac gcctgccaca atccggggcg caatgccgac
    39601 tgttcgccca cgctcaacgc gcgcatcagc ccgatgccgt ctgaaagccc gccgtccgca
    39661 tccgcctgcc gccaattgcg gctgatgcgg ctgcgccaaa ccgcaatccc ccaaccgctt
    39721 ccgccatgca gcaaaaccct gtccgcaccg accgtcccca cgccgcctac cccgttggat
    39781 aatgcccacg cctcacggtt caaaccgcgc aggttcaact ccccgacgac ggggtgcacg
    39841 cgtgccgtta tccgccatct gctcccgacc gcccattcgc gccgtttgta gtccgacagc
    39901 agcaaatcaa acgtccgacc gccgctgtcc acagcctttg ccgcaaactg cacgcgccgc
    39961 ccgtccgacc tcggcatatc cgccacttcc accgtcaaag gcacgcctga aaccgcctcc
    40021 gcccgccatt gcgaagacag tgccgcttcc gttctgaata cgccgtaagc tgcgcccgcc
    40081 aacacgcaca gcatcagacc ggcaaacgca aaacgccttg caagcacagc caaaaccgca
    40141 aaagccgcca accaaaacgg ccaatgcggt acaaccgaca gtgcgaacga aactgccacg
    40201 ccgacaaccc aacacggcaa aaaccaacga cggaacacgt ctccccactc ccgatataaa
    40261 aaacaaacga tgataaagca aagccataaa accgtcataa caagatttga aaatgataag
    40321 aatgcaaacg aaaagcctca agtattatcc gagacaacgg caaactttcc atcgtcattc
    40381 ccgcaaaagc gggaatccaa agacaaaaag ccgcagaaat ttatcggaaa gaaccgaacc
    40441 acaaaaaccg gcttcccacc ttcgcttgaa taacagaaca aagaaagcgg aaataaaaac
    40501 atcgggattc ttttttaaat tggcaataaa acaccaaaat gaatgagtta cacaaaaaaa
    40561 tactcaacac cacccaaccg gcgtaaaatg caaaacatta tcgctattaa aacggtaaaa
    40621 ccttatgaaa gccaaacgtt ttaaaattaa cgccatatcc ttatccatct ttcttgccta
    40681 tgcccttacg ccatactcag aagcggcatt ggtgagagac gatgtcgatt atcaaatatt
    40741 ccgtgacttt gcagaaaaca aaggcaaatt ttttgtcggc gcaaccgatt tatcagtgaa
    40801 aaacaaacaa ggtcaaaaca tcggcaacgc gttatccaac gtaccgatga ttgattttag
    40861 cgttgcagat gtcaacaaac gcatagcaac ggtagtcgat ccccaatatg ccgtcagcgt
    40921 caaacacgcc aaagcagaag tccatacttt ttattacggc caatacaacg gtcataatga
    40981 tgtagccgac aaagaaaatg aataccgcgt tgtcgaacaa aataactatg aaccccataa
    41041 agcttggggt gcgagcaatt taggccgcct cgaagattat aatatggcgc gttttaataa
    41101 gtttgtaacc gaagttgcgc cgattgcacc aactgatgcc ggcggcggat tagatactta
    41161 caaagataaa aaccgttttt cctcttttgt cagaatcggt gcgggcaggc aattagttta
    41221 tgaaaaaggg gtttatcatc aagaaggaaa tgaaaaaggc tacgatttgc gcgatctttc
    41281 acaagcctat cgttatgcca ttgcaggtac gccttataaa gatattaata ttgaccaaac
    41341 aatgaatacc gaaggtttga tcggctttgg taatcataat aaacagtatt cggcagaaga
    41401 acttaaacaa gcactttcgc aagatgcgtt aacaaattac ggcgtgttgg gcgatagcgg
    41461 ctctccgtta tttgcttttg acaaacaaaa aaatcaatgg gtctttttgg gaacttatga
    41521 ttattgggca gggtacggaa aaaaatcttg gcaagaatgg aatatctata aaaaagaatt
    41581 tgcagataaa atcaaacaac gcgataacgc cggcaccgtc aaaggtaatg gagaacatca
    41641 ttggaacatt acttctggca caaacagcaa gattggctct acagcggtaa ggcttgccgg
    41701 caatgaaagg gatgcaaaca acggacagaa tgttaccttt gaagacaacg gcactttggt
    41761 attggatcaa aacatcaacc aaggcgcggg cggcctgttt ttcaaaggcg attacacagt
    41821 caaaggtgca aataatgaca tcacttggct aggtgcgggg attgatgttg ccgacggcaa
    41881 aaaagtcgtt tggcaagtca aaaatccgaa tggcgacaga ttggcaaaaa tcggcaaagg
    41941 cactttggaa ataaacggca caggcgttaa ccaagggcaa ttaaaagtcg gcgacggtac
    42001 ggttattctg aatcaacaag ccgatgccga caaaaaagtc caggctttct cccaagtcgg
    42061 cattgtgagc ggacgcggta cattggtgct gaacagtccg gaccagatta acccgaataa
    42121 tctgtatttc ggtttccgtg gcggtcgttt ggatgccaat ggcaatgact tgacttttga
    42181 acacatccgc aacgtggatg aaggcgcgcg cattgtcaac cacaacacag gccacgcctc
    42241 cacaatcaca ctaacgggta aatctttaat taccaatccg aacagccttt cggtacatag
    42301 catacaaaat gactatgacg aagataatta tagttattat taccggccaa ggcgacccat
    42361 cccacaaggc aaagatcttt attacaaaaa ctatcgttat tacgccctaa aatccggtgg
    42421 cagtgttaac gcaccgatgc ccgaaaacgg acaaacggaa aataacgact ggattttaat
    42481 gggcagcaca caagaagaag ccaagaaaaa cgcgatgaac cataaaaaca atcagcgtat
    42541 tagcggtttt agcggtttct ttggcgaaga aaacgaaaaa ggacataacg gggcattaaa
    42601 ccttaatttc aacggcaaaa gcgcgcaaaa ccgtttcttg ttaacaggcg gcgccaattt
    42661 aaacggaaaa ataagcgtaa ctcaaggcaa tgtcttgtta tcaggtcgtc caacaccaca
    42721 cgcaagagat tttgtgaaca aatcttcagc ccgaaaagac gcacatttct ccaaaaacaa
    42781 tgaagtcgta tttgaagacg actggataaa ccgcacattc aaagccgcag aaattgcggt
    42841 taaccaatcc gcatcattct cttccggaag aaatgtatcc gacatcaccg ccaacatcac
    42901 cgcaacagac aacgccaaag taaatttggg ttacaaaaac ggcgatgagg tttgcgtgcg
    42961 ctcggactat accggttacg ttacctgcaa cacaggcaac ttatccgata aggctttaaa
    43021 cagctttgat gcgacacgga ttaacggcaa tgtgaatttg aatcagaatg cggcattggt
    43081 cttgggcaag gctgcattat gggggaaaat tcaaggacaa ggaaacagcc gtgtcagcct
    43141 aaaccaacat agcaaatggc atttgaccgg cgacagccaa gtacacaatc tgtcattggc
    43201 ggatagccat attcatttga acaacgcttc cgatgcgcaa agtgcaaata aataccacac
    43261 gatcaaaatc aatcatttat ccggtaacgg gcattttcat tatctgacgg acttggcgaa
    43321 aaatcttggg gataaagtgc ttgtgaagga atccgcatcc ggccattatc agctccatgt
    43381 tcaaaataaa acaggcgaac ctaatcagga agggctggat ctctttgatg catcatccgt
    43441 acaagaccgc tcccgccttt ttgtttcctt ggcaaatcat tacgtcgatt taggcgcatt
    43501 gcgttataca atcaaaacag aaaacggtat tacccggttg tacaatcctt atgccgggaa
    43561 ccgccgccca gtcaagccgg ccccgtctcc tgccgcaaac acggcttctc aagcacaaaa
    43621 ggcaacacaa acggacggtg cacaaattgc caagcctcaa aatatcgtcg tcgcaccgcc
    43681 tagcccgcag gcaaatcaag ccgaagaagc cctccgccaa caagcaaaag cggagcaagt
    43741 gaagcgtcag caagcagcag aagcagaaaa agttgcacgt caaaaagacg aagaggcaaa
    43801 acgcaaagca gccgaaattg ctcgtcagca ggaagaagca cgaaaagctg cagagttagc
    43861 cgccaaacaa aaggcggaag cagagcgtaa agccagagag ttggcaagac aaaaagcaga
    43921 agaggcaagt catcaagcta atgccaaacc aaaacgtcgt agacgtcggg ctatattacc
    43981 tagacctcct gcgccagtat tttcattgga tgattatgat gcaaaagaca atagtgaatc
    44041 atcaataggt aatttagctc gtgtaatacc tagaatggga agggagttaa ttaatgatta
    44101 tgaagaaatc cccttggagg agttggaaga tgaagcggaa gaagaacgtc gccaagcaac
    44161 gcaattccaa cccaaaagtc gtaaccgtag agctatatca tcggaaccat catctgatga
    44221 agatgcatct gaatcggttt ccacatcaga caaacaccct caagataata cggaacttca
    44281 tgaaaaagtt gagacggcgg gtttacaacc aagagccgcg cagccgcgaa cccaagccgc
    44341 cgcgcaagcc gatgcagtca gcaccaatac taactcggct ttatctgacg caatggcaag
    44401 cacgcaatct atcttgttgg atacaggtgc ttcattaaca cggcacattg cacaaaaatc
    44461 acgcgctgat gccgaaaaaa acagtgtttg gatgtcaaac accggttatg gccgtgatta
    44521 tgcttccgca caatatcgcc ggtttagttc gaaacgcacg caaacacaaa tcggcattga
    44581 ccgcagcttg tccgaaaata tgcagatagg cggagtattg acttactctg acagtcagca
    44641 tacttttgat caggcgggcg gcaaaaatac ttttgtgcaa gccaaccttt atggtaagta
    44701 ttatttaaat gatgcttggt atgtggccgg cgatattggt gcgggcagct tgagaagccg
    44761 gttacaaacg cagcaaaaag caaactttaa ccgaacaagc atccaaaccg gccttacttt
    44821 gggcaatacg ctgaaaatca atcaattcga gattgtccct agtgcgggta tccgttacag
    44881 ccgcctgtca tctgcagatt acaagttggg tgacgacagt gttaaagtaa gttctatggc
    44941 agtgaaaaca ctaacggccg gactggattt tgcttatcgg tttaaagtcg gcaaccttac
    45001 cgtaaaaccc ttgttatctg tagcttactt tgccaattat ggcaaaggcg gcgtgaatgt
    45061 gggcggtaaa tccttcgcct ataaagcaga taatcaacag caatattcag caggcgccgc
    45121 gttactgtac cgtaatgtta cattaaacgt aaatggcagt attacaaaag gcaaacaatt
    45181 ggaaaaacaa aaatccggac aaattaaaat acagattcgt ttctaaaata ctaaattcat
    45241 agcaaaataa aatgccgtct gaactcaagc ttcggacggc atttttatca gactaacaaa
    45301 gctacagctc aatgcctttg agtttcgcca cggtattgat gtctttgtcg ccgcgacccg
    45361 acaggttgac caaaatcact tggtctttac ccattttcgg cgcgtttttc accgcccaag
    45421 caacggcgtg gctggattcg agcgcgggga tgatgccctc gaatcggcag agcaagtcaa
    45481 aggcttcgag tgcttcgtca tctttggcaa cggtgtattc gacgcgcttg atgtcgtgca
    45541 gatggctgtg ttccgggccg atgccggggt aatccaagcc tgcggaaaca gagtgcgtcc
    45601 ccaagacttg gccgttttcg tcctgcatca gatagctgcg gaagccgtgc agtacgccaa
    45661 tcggtgcgcc cgaagtaatc ggcgcggcgt ggtcaggcgt atccacgccc aaaccgcctg
    45721 cttctacgcc gaccaaacgt acgttttctt cttcgatata gggatagaac aaaccgatgg
    45781 cgttcgagcc gccgccgacg caggcaacgg cgacgtcggg ctgtctgccg atagcttcct
    45841 gcatctgcgc tttggcttcg ttgccgatga cgcattggaa atcacgcacc atttcgggat
    45901 acggcgcggg gccggcggcg gtgccgatga tgtagaacgt atcgtccact cgggcgaccc
    45961 attcgcgcat cgcttcgttc atcgcgtctt tcaaagtacg gctgccactg tcaacgccga
    46021 tgacattcgc accaagcagc ttcatacgga acacattcgg catttggcgt tgaatatcgt
    46081 ccgcacccat gtacacgtcg caagtcatgc cgaaacgtgc ggcgacggtg gcagaagcca
    46141 cgccgtgctg acccgcgccg gtttcggcga tgacgcgttt tttgcccata cggcgggcaa
    46201 gcaatgcctg accgatggtg ttgttgacct tgtgcgcgcc ggtgtggttc aagtcttcgc
    46261 gcttcaacca gatttgcgcg ccgcccaaat gctcggacaa ccgcgcggcg tggtaaacgg
    46321 ggctgggcct gccgacataa tgtttcaaat cgcggcggaa ctccgcccaa aattcaggat
    46381 cgtttttcgc ttcgttgtag gcctgttcca gctctttcaa agcgggaatc agggtttcgg
    46441 aaacataaag cccgccgtgt tcgccgaaaa agcccttttc gtcgggcgcg tggtagttct
    46501 tcatcggatt cttccttatg cttcaggttt tcagacggca tttccgtttt ttcaaacatg
    46561 ccgtctgaac gggaaattgt cggcaattat aactgctttt cacacaagcc cgccatttcg
    46621 aaaaacgccc gatacgccgc tgcctttttc gccaaactca aaggataggc gcgcgaagtc
    46681 gaaggcaggc gcgtcagtgt caaatcccgt ccggcaaacg gaaacggcac ggtttcgccc
    46741 gttttcggca ttttgatgcc gccgccctga atatcgagca ggatttccgt cgccttgccg
    46801 cctgtcgtac aaatatggcg gcagtctggt attttcgcca acaccgccgc caaatcgacg
    46861 gtttcaacta cctttaaaaa cttgtcggac gcattgccgt gctcccttgc cgccttcaac
    46921 acggtcggac aggacgcaat cccccgttcg tgcaaaaacg ccttgatttt ctccgcgtca
    46981 aacgcttttt cagagtgcgc agcctccccg tgggacggca tccggaaatg cgccgcatcg
    47041 ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt ggaaattcgg ataatgaaac
    47101 tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca tcatcatgac cgcagccttc
    47161 ggcggcaaca cgggatcgaa gggatgggtt tcgatttcaa gcagattttc agacacgttc
    47221 aatcctccaa caggcaaagc ggcagacagc cggtttgcac caaaccgcca aacacggcaa
    47281 gccttcaaac agcattatca cgccctgccg ttttcggtta caatgccgac atcaaacgga
    47341 tactgtaaac acatttatgt tccaacacac aggacgacac ataaagcacc gccctatgtg
    47401 tcgtcctgat ttggaagggg ttacgccccg cccaaacaga gtctgatcct gccgccctaa
    47461 agggcggggt ttcaaccgaa gaggaaacac gatgaaagaa cacaaagccc gcaagcgttt
    47521 cgggcagaat tttttgcagg acacgcggat tatcggcgat attgtcaacg ccgtgcgccc
    47581 gcaggcggat gatgtcgtga ttgaaatcgg cccgggtttg gcggcgatta ccgaaccttt
    47641 ggcgaaaaag ctgaaccgcc tgcacgttgt cgaaatcgac cgcgacatcg tatgccgtct
    47701 gaaaacgctg ccgtttgcgg ataaattggt gattcacgaa ggcgatgtat tgcagtttga
    47761 tttcaacaac attgaaggca aaaagaaaat cgtcggcaac ctgccctaca acatttccac
    47821 gccgcttttg ttcaagctgg cggaagtggc ggacgatgtc gccgatatgc actttatgct
    47881 gcaaaaagaa gtggtcgagc gcatggttgc cgcgccgaaa agcaacgact acggtcgctt
    47941 gggtgtgatg ctgcaatatt ttttcgatat ggagctgctg attgacgtgc cgcccgaatc
    48001 gttcgatccc gcgccgaaaa tcgattccgc agtggtgcgc atgattccgg tgaaacaccg
    48061 tatcggcaag gcggacgatt tcgagcattt cgccaaactc gtgaaactcg ccttccacca
    48121 acgccgcaaa accatacgca acaacctgaa agagcttgcc gacgacgatg atttgcaggc
    48181 agtcggcatc agtccgcaag accgtgccga acacatcgcg ccggagaaat atgtggcgtt
    48241 gagcaattat ctggcggaca aggccgtctg aaaagggacg cggcgatgga aagcaacctg
    48301 cagaaaaccg agcgtttgat ccgcgaaatc aaccgcctgc acgcacaata ttcgcaggat
    48361 tattttgaaa ccggcaaagt gcggaaaatc aatttgagcc acacgctcaa aaacgttccg
    48421 accgaacata ttttgtctta ccgccttaat ttgcacgaag cggttaacga ttatttagcc
    48481 tttgccgata cgcgcggcat agactttttc taccgcgtga aaaccgcaga aagcatacat
    48541 gataaaatca accgctatct ggcacgcggc acccaatatc cgaccaacaa catcctgaac
    48601 gatattttcg gcgcgcggct gatttggccg tctgaaacag tcgccgggat tttagaaaaa
    48661 cttgacggct ggaaaacaga atacggcttg aaaaactggt atttgcgcga tgccggcggc
    48721 tatatcggca tacacattta tttcaaaaac agcagcaatt tctactatcc gtgggaattg
    48781 caggtttggg atgaaaaaga tgccaaagcc aatattgaaa accatatggc gtacaaacgg
    48841 aatttcgtgc gttgaattgc cggcaagccg ccttgaaacc gtttataact gtttataaga
    48901 gaataaacca taaaatacac aaccgtattc ctgacttttc ctttaaccgc ccgagtgaac
    48961 aaactatgaa atacacagcc gcactcctga cttttctttt aactgcctgc atgaacccga
    49021 acgatgcgtt tttccaaaac cggcgttatc aaatgccgga ggcgcaattg aacggttcaa
    49081 acgccgtttt ccattacggt tattcccaaa atcccgatca tgatttattg gtcgaccaag
    49141 tccgcgtgcc ggacgcgtac agggcttcat ttgcgcggga ttggatagcg aaggagcaag
    49201 gttaccgcaa aaacgactgc gtactggaaa aagcggaaag cgggggtgtg acgtactaca
    49261 cctgcgatgc ggaaaaagga ttcaacggca cacgtttcgt catctatgtc gtgaatatga
    49321 aaaacgacaa aggctacgtc aaagtttacc ggggcatgaa taagccgaat caggaagagt
    49381 taaccaaatt ggttgccgat ttgaataagt tttaccctta atcagccaga cggattcaga
    49441 ctgcccgaaa agaatggaca ctatgatcaa attcaaaaac gtacacaaac atttcaaaga
    49501 cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa ggcgaagtgg tcgtcgtctg
    49561 cgggccttcg ggcagcggca aatccacact catccgcacc gtcaaccagc ttgaaagcat
    49621 cgaaagcggc gagatttggg tggacggcgt caacgttgcc gaccccaaaa ccgatttgaa
    49681 caaaatccgc gaagaagtcg gctttgtgtt tcaaagtttc aacctctacc cgcacctgac
    49741 cgtgttggac aacatcaccc tcgcaccgat gaaggttaag gggcaaaatg cggaacaggc
    49801 ggagaaaaag gcaatggagc ttttggaacg cgtcggactg gcacacaaaa aagacgcctt
    49861 cccttcccaa ctttccggcg gccagcagca acgcgtggcg attgcgcgcg ggctggcgat
    49921 ggagccgcgc gtgatgctgt ttgacgagcc gacttccgca ctcgaccccg aaatggtcgg
    49981 cgaagtgttg aaagtgatga aagacttggc ggaaagcggc atgacgatga tgtgcgtaac
    50041 ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg attttcgtcg ataaagggca
    50101 aatccttgaa gacgaaacgc cggaagcgtt tttcaccaac ccgaaacacg aacgcgccaa
    50161 gcagttcctg caacaagtca tgacccattg atgctatgcc gtctgaaaac cgaacgttca
    50221 gacggcatag cccgacaccg caagatgatt gagaaagcac accatgtatt ttgttgaccg
    50281 caccgccgcc gtcctcaagc cgaccgcgcg ctttttggaa tggctcaaaa gcaccgacga
    50341 aaatatgccc gacctgacca tagaacagct tcgcgccgac tgttccgtct tcctcgttcc
    50401 gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac gaacgctacc ggcagatttt
    50461 tgaggcggag ctggcaggtt gggacatcga caaggacaaa tggccgcaag atatggggct
    50521 gaaggcgttt tgggaatttt tcgacattga aatccacgat atggttttgg atatggaaga
    50581 agcggatttg aacatcacgc ccgtgttcga caatatgatg taacgccatg cgcgcctttc
    50641 agacggcatt gaggccgtcc cgcatcctga acatcctgac cgtatcactc cactcggctt
    50701 ccattgccgt ctgcctgact tggttttacg gccggatgat gtggttcggt ttggcggctt
    50761 tggcggcaag ttacgcatac tcgctcagga taacgaatct gaaacaccgc catgccataa
    50821 ccgccattac catagaccgc gacggacggg cggaaatcgt atccggcaaa gacaaaacag
    50881 cggcaacact tgcgggcagc agtatggtta cgccttatgc cttattctta caatgggaca
    50941 caggcggcaa aaccgtccgc caatgcatca tgcccgacat gacggacaaa gaatcctacc
    51001 ggcggctgaa agtctgggtg ctttggcggc agccgaaaaa aaccgccgaa accgatacat
    51061 cagactgatt tcagacggca tttgaaaacg aaacactcat gaaaacacta caagactggc
    51121 tctcgcattt ggaaaccgcc cacagcggcg gtttgatcga catgggtttg gagcgcgtaa
    51181 gcgaagtaaa aaagcgcatg aacctgacac cgcaatgccc cgtcgtcgtc gtcgcgggaa
    51241 caaacggcaa aggttcggtc tgcgcctacc tgacacagat ttacaaacag gcaggcttca
    51301 aaaccggcac gctgaccagc ccgcatttat tgcattacaa cgaacgcatc gccatcaacg
    51361 ccgaacccgt ttcggacgat acgattattg cctctttcga gcgcatcgaa gccgcgcgcg
    51421 gcgaaatatc gctgacttat tttgaattca atgccttggt ggctgtcgac atcttcatac
    51481 gcgaacaagt ggatgtaatg atattggaag tcggcttggg cggacgtttg gacgcggtca
    51541 acgcgtttga cggcgattgc gcggtggtta ctagtgtgga tttggaccat caggcatttt
    51601 tgggcgatac ggtcgaacag gtcggctttg aaaaggcagg cgtgttccgc agcggcaaac
    51661 ccgccatctg cggtcaaaac cccgcgcccg catcattggt cgcacacgcc gaagccatag
    51721 gcgcgaaact gctgatggta cagcgcgatt tcgagtttca cgcgatggaa aacatccaat
    51781 ggaactaccg cttccggccg cagcattcag acggccccgc acgcaaccgc aatgccctgc
    51841 ccttccccgc attgcgcggc gcataccagc tttctaacgc cgcctgcgcg ttgaccgtat
    51901 tggaatgcct cgacgacaga ctgccggtgg acattggtgc aatcaaacgc gggctgctgc
    51961 tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg gccgctgacc gtattggatg
    52021 tcggacacaa cccccacgcc gcccgcgccc tgcgccgcaa cctgatcaac cttgcctacg
    52081 cgcaaaagcg caccgccgtg ttcagtatgc tgtccgacaa agacatagac ggcgtgtcgg
    52141 aaaccgttaa agaccagttt gacgagtggt atatcgcgcc tttggacgtg ccgcgcggta
    52201 tgacggcgga tgcactgaaa gccaaattgg aacaacacca catcgaaaac atacagacat
    52261 tcgccgccgt ccgcgacgcg taccgggcgg cagcctcaaa agcgggcgaa gacgacagaa
    52321 tcgtcgtatt cggctcattt cataccgtcg ccgacgtaat gtcggtactg tgaaaaggaa
    52381 acttctatgt ccgaaaacaa acaaaacgaa gtcctgaccg gttacgaaca gctgaaacgg
    52441 cgcaaccgcc gccgcctcgt aacggcaagc tccctggttg ccgcctcctg catcctgctg
    52501 gcagccgcac tcagttccga tcctgccgac agcaatcccg caccgcaggc cggcgaaacc
    52561 ggcgcaacgg aaagccaaac ggcaaacacg gcacaaaccc ctgccttgaa atccgccgcc
    52621 gaaaacgggg aaaccgccgc cgacaaaccg caggacttgg caggcgaaga caagccttct
    52681 gccgccgaca gcgaaatcag cgagcctgaa aacgtaggcg cgccgctggt gctgattaac
    52741 gaccggctcg aagacagcaa catcaaaggt ttggaagaat ccgagaaact gcaacaggca
    52801 gaaaccgcca aaaccgaacc gaagcaggca aaacaacgcg ctgccgaaaa agtgtcggca
    52861 actgccgaca gtacggatac ggtagcggtt gaaaaaccga aacgcactgc cgaacccaaa
    52921 ccgcaaaaag cggaacgcac tgccgaagcc aagcccaaag ccaaagaaac caaaaccgcc
    52981 gaaaaagttg ccgacaaacc gaaaactgct gccgaaaaaa ccaaaccgga tacggcaaaa
    53041 tccgacagcg cggtaaaaga agcgaaaaaa gccgacaagg ctgaaggcaa aaagacagcc
    53101 gaaaaagacc gttcggacgg caaaaaacac gaaacggcgc aaaaaaccga caaagcggac
    53161 aaaaccaaaa ccgccgagaa ggaaaaatcc ggcaaggcgg gcaaaaaagc cgccattcag
    53221 gcaggttatg ccgaaaaaga acgcgccttg agcctccagc gcaaaatgaa ggcggcgggt
    53281 atcgattcga ccatcaccga aatcatgacc gacaacggca aagtttaccg cgtcaaatca
    53341 agcaactata aaaacgcaag ggatgccgaa cgcgatttga acaaactgcg cgtgcacggc
    53401 atcgccggcc aggtaacgaa tgaatagcct ccccattgcc gacctcctcg cctccgccgt
    53461 catcgccgcc tgcatcgtca tttccacgat gcgcggcgtg attgcggaag caggttcgat
    53521 ggtggcatgg gtggtttcct tcttttttgc caaactcttt gccgcaccct tcgccgacct
    53581 cgcctttgcc tcgttccaac cccgcctgtt tgcattggct ctgtcattca tttccctgtt
    53641 cgtcattgcc tgtctgatcc agaaaatgct ccgttcgctg ctgaccggtg cagtttcggc
    53701 ggtcggtctg ggctttgcca accgcatttt gggcggtgta ttcggtgcat tgaaaggcgt
    53761 tttgattgtt accctgctga tcatgcttgc ttcaaaaacc gacctgcccg ataccgaaga
    53821 atggcaacag tcctataccg taccgttttt cgtatcgctt tccgaagcgg tgttaaacca
    53881 tacggacaac gcacccgaat ccctcgacga cgactaatcc gcatacgtgg cgtttcagac
    53941 agcatacctg atttatgccg tccgaaacgc cttttctttc cttgacacac cccgacagac
    54001 aaagcgcacc tgcctgactt tacacaaata ttgtcaacaa ttcgggcaaa aagccgccgt
    54061 ttgctttata attccgtttt acccttaaac ctcgaatcgg agaagacgat atgtgcggcg
    54121 tattaggttt ggtcagtcat gaacccgtga accagcttct gtacgacggt ttgcagatgt
    54181 tgcagcacag ggggcaggat gcggcgggca ttgcaacggc ggaaggcggt accttccata
    54241 tgcacaaagg caaagggatg gtgagcgaag tgttccgcac gcgcaatatg cgcgatttga
    54301 ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc gggcaacgcc ggcagcagcg
    54361 cggaggcgca gcctttctac gtcagctcgc ctttcggcat cgttttggcg cacaacggca
    54421 acctcaccaa cactgccgaa ctgtatgaaa acgtgtgtaa caaacacctg cgccacatca
    54481 acaccggctc cgattccgaa gttttgctca acgtattcgc gcacgaattg cgccgcgaag
    54541 tctctaaaaa cgccgacccg caccggctca atgccgacaa tattttcaac gccgttgccg
    54601 aagtccaccg cctggtgcgc ggtgcatacg gcgtggttgc catgattgcg ggctacggca
    54661 tggttgcctt ccgcgaccct tacggcatcc gcccgctggc attgggttcg caaaccgaca
    54721 gcgaaggcag aaaatcctat gccgtcgcct ccgaatccgt cgccttcaac gcgcttgcct
    54781 acgatttgga acgcgatatc cggccgggcg aagcggtatt tgtcggcttt aacggcacaa
    54841 tcatcgcccg acaatgcagc gaccgcgcca aactcagccc ctgccttttt gaatatgttt
    54901 attttgcccg ccccgactcc gtgattgacg gcgtgtccgt ttaccagtcg cgcttggata
    54961 tgggcgtgtc cttggcggaa aaaatcaaac gcgagctgcc cgtggacggc atcgacgtcg
    55021 tgatgcccat tcccgacacc agccgcccca gtgcaatgga acttgccgtc cacctcaaaa
    55081 agccttaccg cgagggtttg attaaaaacc gctatatcgg ccgcaccttt atcatgcccg
    55141 gtcagtcgac gcgcaaaaaa tccgtgcgcc aaaaactcag cccgatggaa accgaatttg
    55201 caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg cgggacgacc agccgcgaaa
    55261 tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta tatcgcctcc gccgcgcccg
    55321 aagtgcgcta tcccaatgtg tacggcatcg atatgcccac gcgcgaagag ttgattgcca
    55381 acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc ggacggcatc gtgtttcaag
    55441 acttgggcga tttggaagcc gtcgtcaaag cactcaaccc gaaaatcgaa tccttcgatt
    55501 cgtcctgttt caacggcatt tatcagaccg gcgacatcga tgatgcctac ctcgaccgcc
    55561 tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca cccgagcagg atggaacaca
    55621 gcatcagcat cagcatcagc gatgcgggcg acgaagaata aggcttccca aaccgcgatg
    55681 ccgtctgaaa tccgaacgtt gggttttcag acggcatttc ccatatcggc atcacatttc
    55741 ccgtttcatc cgcacacaat atgagtatcg aaaccaaaaa ctacattacg cccgccggct
    55801 ggcaggcatt gaaagacgag ctttatcaac tggtcaataa agaacgcccc gaaatcgtcc
    55861 aaatcgtcaa ctgggcggca ggcaacggcg accgcagcga aaacggcgac tatctttacg
    55921 gcaaacgccg tatgcgcgaa atcgaccgcc gcatccgttt cctgaccaaa cgtttggagg
    55981 ctgccgtcgt cgtcgatccc gaattgcgcg aagcgaccga ccaagtattt ttcggcgcga
    56041 ccgtcggtct gttgcgcggc gacgggcgcg agcaaaccgt caaaatcgtc ggcgtcgatg
    56101 aaatcgatac cgcgcaaaac aaaatttctt ggatttcgcc gttggcgcgt tgcctgatta
    56161 aagcgcgcga aggcgacgaa gtggttttga acacgccgga aggccgtgag gaaattgaaa
    56221 tcctttctgt cgaatacatc aaaatcgact gatcgggaat gccgtctgaa acggcacaag
    56281 cgcaccgttt cccaaataaa ggaataccga atgaggaaaa tccgcaccaa aatctgcggc
    56341 atcaccacac cggaagacgc actgtatgcc gcccacgccg gcgcagacgc attgggactg
    56401 gttttttacc cccaaagccc ccgcgctatc gacatcatta aagcacaaaa aatcgccgcc
    56461 gcactgccgc cgtttgtcag cgttgtcgcc cttttcgtca acgaaagcgc gcaaaacatc
    56521 cgccgcatcc ttgccgaagt gccgatacac atcatccaat tccacggcga cgaagacgat
    56581 gcattctgcc ggcagttcga ccgcccctat attaaagcca ttcgtgttca gacggcatca
    56641 gacatccgaa acgccgccac gcgcttcccc aacgctcagg cactgctgtt cgatgcctat
    56701 cacccttcgg aatacggcgg caccggacac cgcttcgact ggacgctgtt ggcggaatat
    56761 tcgggcaagc cgtgggtgct tgccggcggg ctgacccctg aaaacgtcgg cgaagccgtc
    56821 cgcatcaccg gagcggaagc ggtcgacgta tccggcggcg tggaagcgtc taaaggcaaa
    56881 aaagaccccg ccaaagtcgc cgcctttatc gcaaccgcca accgcctatc ccgttaaagc
    56941 aacaaaaatt gccgccggaa tgacttatag tggattaaca
//
LOCUS       DAXFHF010000003        54872 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXFHF010000003 DAXFHF010000000
VERSION     DAXFHF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 54872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54872
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.003"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            180..800
                     /locus_tag="ACFIRM_000123"
     CDS             180..800
                     /locus_tag="ACFIRM_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC3027865.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            823..2276
                     /locus_tag="ACFIRM_000124"
                     /pseudo
     CDS             823..2276
                     /locus_tag="ACFIRM_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
     gene            2737..3093
                     /locus_tag="ACFIRM_000125"
     CDS             2737..3093
                     /locus_tag="ACFIRM_000125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC3027866.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            3084..3566
                     /locus_tag="ACFIRM_000126"
     CDS             3084..3566
                     /locus_tag="ACFIRM_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC3027867.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            3579..4172
                     /locus_tag="ACFIRM_000127"
     CDS             3579..4172
                     /locus_tag="ACFIRM_000127"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC3027868.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            4162..5418
                     /gene="nuoD"
                     /locus_tag="ACFIRM_000128"
     CDS             4162..5418
                     /gene="nuoD"
                     /locus_tag="ACFIRM_000128"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC3027869.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            5418..5891
                     /gene="nuoE"
                     /locus_tag="ACFIRM_000129"
     CDS             5418..5891
                     /gene="nuoE"
                     /locus_tag="ACFIRM_000129"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC3027870.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            6281..7582
                     /gene="nuoF"
                     /locus_tag="ACFIRM_000130"
     CDS             6281..7582
                     /gene="nuoF"
                     /locus_tag="ACFIRM_000130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC3027871.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            7582..7881
                     /locus_tag="ACFIRM_000131"
     CDS             7582..7881
                     /locus_tag="ACFIRM_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027872.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            8295..10556
                     /gene="nuoG"
                     /locus_tag="ACFIRM_000132"
     CDS             8295..10556
                     /gene="nuoG"
                     /locus_tag="ACFIRM_000132"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC3027873.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEITMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPPLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASNRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            10559..11635
                     /gene="nuoH"
                     /locus_tag="ACFIRM_000133"
     CDS             10559..11635
                     /gene="nuoH"
                     /locus_tag="ACFIRM_000133"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC3027874.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            11716..12195
                     /gene="nuoI"
                     /locus_tag="ACFIRM_000134"
     CDS             11716..12195
                     /gene="nuoI"
                     /locus_tag="ACFIRM_000134"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC3027875.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            12207..12878
                     /locus_tag="ACFIRM_000135"
     CDS             12207..12878
                     /locus_tag="ACFIRM_000135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC3027876.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            12875..13180
                     /gene="nuoK"
                     /locus_tag="ACFIRM_000136"
     CDS             12875..13180
                     /gene="nuoK"
                     /locus_tag="ACFIRM_000136"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC3027877.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            13183..15207
                     /gene="nuoL"
                     /locus_tag="ACFIRM_000137"
     CDS             13183..15207
                     /gene="nuoL"
                     /locus_tag="ACFIRM_000137"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC3027878.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFINADAHPTMHIMKEEFHGALAMVSHSLTSPVLYLAAAGVAAAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15391..16554
                     /locus_tag="ACFIRM_000138"
     CDS             15391..16554
                     /locus_tag="ACFIRM_000138"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC3027879.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            16668..18164
                     /locus_tag="ACFIRM_000139"
     CDS             16668..18164
                     /locus_tag="ACFIRM_000139"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC3027880.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            18174..19619
                     /gene="nuoN"
                     /locus_tag="ACFIRM_000140"
     CDS             18174..19619
                     /gene="nuoN"
                     /locus_tag="ACFIRM_000140"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC3027881.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            19731..20030
                     /locus_tag="ACFIRM_000141"
     CDS             19731..20030
                     /locus_tag="ACFIRM_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC3027882.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(20147..21043)
                     /locus_tag="ACFIRM_000142"
     CDS             complement(20147..21043)
                     /locus_tag="ACFIRM_000142"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3027883.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(21027..21251)
                     /locus_tag="ACFIRM_000143"
     CDS             complement(21027..21251)
                     /locus_tag="ACFIRM_000143"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC3027884.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(21364..22287)
                     /gene="rsgA"
                     /locus_tag="ACFIRM_000144"
     CDS             complement(21364..22287)
                     /gene="rsgA"
                     /locus_tag="ACFIRM_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC3027885.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLANALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(22292..24148)
                     /locus_tag="ACFIRM_000145"
     CDS             complement(22292..24148)
                     /locus_tag="ACFIRM_000145"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3027886.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(24174..24311)
                     /locus_tag="ACFIRM_000146"
     CDS             complement(24174..24311)
                     /locus_tag="ACFIRM_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027887.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(24311..24895)
                     /gene="ruvA"
                     /locus_tag="ACFIRM_000147"
     CDS             complement(24311..24895)
                     /gene="ruvA"
                     /locus_tag="ACFIRM_000147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC3027888.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDRLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(24977..25303)
                     /locus_tag="ACFIRM_000148"
     CDS             complement(24977..25303)
                     /locus_tag="ACFIRM_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3027889.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(25364..26050)
                     /locus_tag="ACFIRM_000149"
     CDS             complement(25364..26050)
                     /locus_tag="ACFIRM_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC3027890.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(26178..26642)
                     /gene="trmL"
                     /locus_tag="ACFIRM_000150"
     CDS             complement(26178..26642)
                     /gene="trmL"
                     /locus_tag="ACFIRM_000150"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC3027891.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(26707..27433)
                     /locus_tag="ACFIRM_000151"
                     /pseudo
     CDS             complement(26707..27433)
                     /locus_tag="ACFIRM_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            27432..28181
                     /gene="bioH"
                     /locus_tag="ACFIRM_000152"
     CDS             27432..28181
                     /gene="bioH"
                     /locus_tag="ACFIRM_000152"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC3027892.1"
                     /translation="MPDAVKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEAFAALCRDFVEGGLT"
     gene            28178..28966
                     /locus_tag="ACFIRM_000153"
     CDS             28178..28966
                     /locus_tag="ACFIRM_000153"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3027893.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNVPGHILLAGADADISRS
                     LLAKRYPPAVFEEYDSRADFLAAAAAVRKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLMLPQGENVVRFFPKR"
     gene            complement(29047..29154)
                     /locus_tag="ACFIRM_000154"
                     /pseudo
     CDS             complement(29047..29154)
                     /locus_tag="ACFIRM_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            29276..30649
                     /locus_tag="ACFIRM_000155"
     CDS             29276..30649
                     /locus_tag="ACFIRM_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC3027894.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     PWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(30686..32989)
                     /gene="recQ"
                     /locus_tag="ACFIRM_000156"
     CDS             complement(30686..32989)
                     /gene="recQ"
                     /locus_tag="ACFIRM_000156"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC3027895.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPKGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(33052..33834)
                     /gene="trpC"
                     /locus_tag="ACFIRM_000157"
     CDS             complement(33052..33834)
                     /gene="trpC"
                     /locus_tag="ACFIRM_000157"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC3027896.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(33885..34841)
                     /locus_tag="ACFIRM_000158"
     CDS             complement(33885..34841)
                     /locus_tag="ACFIRM_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC3027897.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(34974..36512)
                     /gene="murJ"
                     /locus_tag="ACFIRM_000159"
     CDS             complement(34974..36512)
                     /gene="murJ"
                     /locus_tag="ACFIRM_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC3027898.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            36950..37648
                     /locus_tag="ACFIRM_000160"
     CDS             36950..37648
                     /locus_tag="ACFIRM_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3027899.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(37705..38709)
                     /gene="amgK"
                     /locus_tag="ACFIRM_000161"
     CDS             complement(37705..38709)
                     /gene="amgK"
                     /locus_tag="ACFIRM_000161"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC3027900.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            38779..41184
                     /locus_tag="ACFIRM_000162"
     CDS             38779..41184
                     /locus_tag="ACFIRM_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC3027901.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSKV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSHDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKFQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            41286..42287
                     /locus_tag="ACFIRM_000163"
     CDS             41286..42287
                     /locus_tag="ACFIRM_000163"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3027902.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(42765..44645)
                     /locus_tag="ACFIRM_000164"
     CDS             complement(42765..44645)
                     /locus_tag="ACFIRM_000164"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HFC3027903.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIVANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGLFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLLSLIDD
                     TAEPLFQQSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            44801..45433
                     /locus_tag="ACFIRM_000165"
     CDS             44801..45433
                     /locus_tag="ACFIRM_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC3027904.1"
                     /translation="MDNVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQNHT
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            45505..46875
                     /gene="purB"
                     /locus_tag="ACFIRM_000166"
     CDS             45505..46875
                     /gene="purB"
                     /locus_tag="ACFIRM_000166"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC3027905.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQQARETVILPKLAEIIGKLTDMAHEL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFSRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            46955..48824
                     /locus_tag="ACFIRM_000167"
                     /pseudo
     CDS             46955..48824
                     /locus_tag="ACFIRM_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(48960..49574)
                     /locus_tag="ACFIRM_000168"
     CDS             complement(48960..49574)
                     /locus_tag="ACFIRM_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC3027906.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGI"
     gene            complement(49610..51760)
                     /gene="dinG"
                     /locus_tag="ACFIRM_000169"
     CDS             complement(49610..51760)
                     /gene="dinG"
                     /locus_tag="ACFIRM_000169"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC3027907.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPDFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(51941..53239)
                     /locus_tag="ACFIRM_000170"
     CDS             complement(51941..53239)
                     /locus_tag="ACFIRM_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC3027908.1"
                     /translation="MPSETALPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(53229..54140)
                     /locus_tag="ACFIRM_000171"
     CDS             complement(53229..54140)
                     /locus_tag="ACFIRM_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3027909.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            54248..54646
                     /locus_tag="ACFIRM_000172"
     CDS             54248..54646
                     /locus_tag="ACFIRM_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3027910.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSS
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            54695..>54872
                     /locus_tag="ACFIRM_000173"
     CDS             54695..>54872
                     /locus_tag="ACFIRM_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3027911.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEE"
BASE COUNT        11887 a        14353 c        15607 g        13025 t
ORIGIN      
        1 aatttttata acgtcatgaa gccgcacagc agtttgaaca gtgatacgcc gtttgcgggc
       61 ttacgagttt attttcccgg cctgcagttt gagcaatgcg gtgatttcct acggctaata
      121 caaatgttta cacattaata catttcattt atagttccgc ctatttgaaa atagaaaata
      181 tgaattcgac cgcaagtaaa accctgaaag gattgtcgct ggtgtttttc gcctctggct
      241 tctgcgccct gatttaccag gtcagctggc agaggcttct attcagccac ataggtatcg
      301 atttgagttc gattactgtc attatttctg tatttatggt cggcttgggt gtaggtgcgt
      361 atttcggcgg acgcattgct gaccgttttc cttcaagtat catccccctg ttttgcatcg
      421 ctgaagtatc catcggtctg ttcggtttgg taagcaaggg tctgatttcc ggcttggggc
      481 atcttttagt tgaggctgat ttgcccatca tcgctgctgc caatttcctc ttattgctgc
      541 ttcctacctt tatgatgggc gcgaccttgc ccttgctgac ctgttttttt aaccggaaaa
      601 tacataatgt tggcgagtct atcggtacct tatatttttt caacactttg ggtgcggcac
      661 tcggatcgct tgccgccgcc gaatttttct acgtcttttt taccctctcc caaaccattg
      721 cgctgacagc ctgccttaac cttctgattg ctgcttcagt atggctgcgt tacagaaagg
      781 atggatatgg tgaacactaa accgaatact agtttgattt atatgctttc tttccttagc
      841 ggattattga gcttgggtat agaagtcttg tgggtaagga tgttttcgtt cgcagcacag
      901 tccgtgcctc aggcattttc atttactctt gcctgttttc tgaccggtat cgccgtcggc
      961 gcgtattttg gcaaacggat ttgccgcagc cgctttgttg atattccctt tatcgggcag
     1021 tgcttcttgt gggcgggtat tgccgatttt ttgattttgg gtgctgcgtg gttgttgacg
     1081 ggtttttccg gcttcgtcca ccacgccggt atcttcatta ccctgtctgc cgtcgtcagg
     1141 gggttgattt tcccacttgt acaccatgtg ggtacggatg gcaacaaatc cggacgacag
     1201 gtttccaatg tttatttcgc caacgttgcc ggcagtgcat tgggtccggt ccttatcggc
     1261 tttgtgatac ttgatttctt gtccacccaa cagatttacc tgctcatctg tttgatttct
     1321 gctgctgtcc ctttgttttg tacaccgttc caaaaaagtc tccgactgaa tgcagtgtcg
     1381 gtagcagttt ccctaatgtt cggcatcctc atgttcctac tgccggattc tgtctttcaa
     1441 aatattgctg gccgtccgga taggttgatt gaaaacaaac acggcattgt tgcggtttac
     1501 catagagatg gtgataaggt tgtttatggg gcgaatgtat acgacggcgc atacaatacc
     1561 gatgtattca atagtgtctc aacggcatcg aacgtgccta tctgccaccc tccctgaagt
     1621 ccggcatacg ccgcattttc gtcgttggat tgagtacagg ttcgtgggcg cgcgtcttgt
     1681 ctgccattcc ggaaatgcag tcgatgatcg ttgcggaaat caatccggca taccgtagcc
     1741 ttatcgcgga cgagccgcaa atcgcaccgc ttttgcagga caaacgtgtt gaaattgtat
     1801 tggatgacgg taggaaatgg ctgcgtcgcc atcctgatga aaaattcgac ctgattttga
     1861 tgaattcgac ttggtactgg cgtgcctatt ccactaacct gttgagtgcg gaatttttaa
     1921 aacaggtgca aagccacctt accccggatg gtattgtaat gtttaatacc acgcacagcc
     1981 cgcatgcttt tgctaccgcc gtacacagta ttccctatgc ataccgctac gggcatatgg
     2041 tagtcggctc ggcaaccccg gtagttttcc ctaataaaga actgctcaag caacgccttt
     2101 cccggttgat ttggccggaa agcggcaggc acgtatttga cagcagcacc gtggatgctg
     2161 cagcacaaaa ggttgtctct cgtatgctga ttcggatgac ggaaccttcg gctggggcgg
     2221 aagtcattac tgacgataat atgattgtag aatacaaata cggcagaggg atttaaccgt
     2281 cttaaagggt tttaggcaac gcaggtttta ggtaacgtcc tgctagttca aaaaaaccgc
     2341 atcacagcag tcgggacaaa atggtttaaa cattttgtcc cgaattctta ttcctatata
     2401 tagcggatta acaaaaatca ggacaaggcg gcgggccgca ggcagtacaa atagtacgga
     2461 accggttcgc ttggtgcttg ggcaccttag ggaatcgttc cccttgagct aaggcggggc
     2521 aacgccgtac cggtttttgt taatccgcta taccacgaat tacggtgtaa aaatttatat
     2581 gaccttataa aatcaaataa gaatcgttat cataacatga ttgtatttat tgggtttttt
     2641 tgggcgtttt gccgatattt accttttaat ggtttttgaa attcgctaaa atacgaaatt
     2701 attgtagaaa ttttgttaac ggatttgggt gtaaccatgt tgtccgctta ctttcccgtc
     2761 tttgtcttta tcctcgtcgg cctcgcggcc ggcgtactgt ttatcctgct cggcacgatt
     2821 ttgggcccga aacgccacta tgccgaaaaa gacgcgcctt acgaatgcgg ttttgaagcc
     2881 tttgaaaacg caaggatgaa gttcgacgtg cgctattacc tcgtcgccat cctgttcatc
     2941 ctcttcgatt tggaggttgc gtttatgctg ccgtgggcag tcgtgttcaa agatttgggc
     3001 gcgtacggct tttggtctat gctggtgttt atcgtcgtcc tgacggtagg ctttgtttac
     3061 gaatggaaaa aaggtgcgct ggaatgggaa tagaaggcgt tttgaaaaaa ggtttcatca
     3121 ccaccagcgc ggatacggtg ttgaactata tgcgtaccgg ttcattgtgg ccggttactt
     3181 tcggattggc ctgctgcgcc gtggaaatga tgcatgcggg tatggcgcgt tatgaccttg
     3241 accgtttcgg tatcattttc cgtccgtccc cacgtcaggc cgacctgatg attgtggcag
     3301 gtacgctgac caataaaatg gcgccagccc tgcgtcgcgt gtacgaccag ctcgccgagc
     3361 cgcgctgggt attgtctatg ggttcgtgtg ccaacggcgg cggctattat cactattctt
     3421 attccgttgt gcgcggtgcc gaccgtgtcg tgccggtgga cgtttatgtg ccgggttgtc
     3481 cgccgactgc ggaagctctg atttacggcc tgattcagct ccaacaaaaa atcaagcgca
     3541 cttccaccat cgcgcgtgac gagtaaggag aggacgatat ggcaagcatt caaaacttat
     3601 acgagaccgt cgtcggcgtg cttggcgatc aggcaggcaa agtcatttca gctttgggcg
     3661 agattaccgt cgagtgtctg cccgagcact atatttcagt catgaccgca ttgcacgacc
     3721 atgaagattt gcatttcgag cttctggttg acttgtgcgg cgtcgattac agcacttaca
     3781 aaaacgaagc atggcagggc aaacgctttg ccgttgtcag tcagttgctt tccgttaaaa
     3841 acaatcaacg catccgcgta cgcgtctggg tttcagacga cgacttccct gtagtcgaat
     3901 ccgtagccga tatttacaac agcgcggatt ggtacgagcg cgaagccttc gatttgtacg
     3961 gcatcatgtt caacaaccat cccgacttgc gccgcatcct gaccgattac ggctttgtcg
     4021 gacatccgtt ccgcaaagac ttcccgattt ccggctatgt ggaaatgcgt tatgacgaag
     4081 agcaaaaacg cgtgatttac caacctgtta ccattgagcc gcgcgaaatc acaccgcgta
     4141 tcgtccgtga ggagaactac ggtggccaat aaattaagaa actacaccat caacttcggc
     4201 ccgcagcacc ctgcggcgca tggcgtattg cgtatgattt tggagctgga cggcgaacaa
     4261 atcgtccgtg ccgatccgca tatcggcctc ctgcaccggg gcaccgaaaa actggcggaa
     4321 accaaaacct atctgcaagc cctgccctat atggaccgtt tggactacgt ttccatgatg
     4381 gtcaacgagc aggcgtattg cttggcggta gaaaaacttg ccggtatcga tgtgcctatc
     4441 cgcgcccaat acatccgcgt gatgttcgcc gaagtaacgc gcatcctcaa tcacttgatg
     4501 ggcatcggtt cgcacgcctt cgacatcggc gcgatgaccg ccatcctcta cgccttccgc
     4561 gaccgcgaag agctgatgga cttgtacgaa gccgtgtccg gcgcacgtat gcacgccgcc
     4621 tacttccgtc ccggcggcgt ttaccgcgac ctacccggct ttatgcccaa atacgagagc
     4681 agcaaattcc gcaatgccaa agtattgaag cagctcaacg aatcccgcga aggcacgatg
     4741 cttgacttta tcgatgcctt ctgcgaacgc ttccccaaaa atatcgacac actcgaaacc
     4801 ctcctgaccg acaaccgcat ctggaaacag cgtaccgtcg gcatcggcgt tgtctctccc
     4861 gagcgcgcca tgcaaaaagg ctttaccggt gtgatgcttc gcggttccgg cgtggaatgg
     4921 gacgtgcgta agacacagcc ctacgaagtg tacgacaaaa tggatttcga catccccgtc
     4981 ggcgtcaacg gcgactgcta cgaccgttac ctctgccgta tggaagaaat gcgccaatcc
     5041 gtacgcatca tcaaacaatg cgccgactgg ttgcgcgtca atcccggccc ggtcattacc
     5101 gcaaaccaca aattcgctcc gcccaaacgt accgaaatga aaacaggtat ggaagacctg
     5161 attcaccatt tcaaactctt taccgagggt atgcacgttc ccgagggcga gacctacacc
     5221 gctgtcgaac acccgaaagg cgagttcggc gtttacatca tttcagacgg cgcaaacaaa
     5281 ccctaccgcc tgaaaatccg cgcacccggc ttcgcccacc tgcaaggcat ggacgaaatg
     5341 gcaaaaggcc atatgctcgc cgacgtcgtt gccatcatcg gtacgcagga catcgtattc
     5401 ggggaggttg accgataatg ttatccgcaa aatccttaaa acaaatcgac attgagttgg
     5461 cgaaatatcc cgccgaccaa cgccgttccg ccatcatggg cgcgttgcgt atcgcccaaa
     5521 ccgaaaaagg ctggctcgcc cccgaaacca tcgcttttgt tgccgactac atcggcatca
     5581 cgccggcgca ggcatatgaa gtcgccactt tctacaatat gtacgacctt gagcctgtcg
     5641 gcaaatacaa actgaccgtt tgtaccaacc tgccctgcgc tctgcgcggc ggtatggcga
     5701 ccggcgaata cctcaaacaa aaactcggta tcggctacgg cgaaaccacg cccgacggca
     5761 aatttaccct tgtcgaaggc gaatgcatgg gcgcgtgcgg cgatgccccc gttatgctgg
     5821 tcaacaacca cagcatgtgc agctttatga ccgaagaggc gattgagaag aaactggcgg
     5881 agttggagta ggtcgtctga aacgacgatt taaacgtagg tcggatactt gtatccgaca
     5941 gagtgggtaa aaaggcaaaa tgtcggattt aagaatccgc cctactgaaa tactgaaatg
     6001 ccgtcattcc cgcgcaggcg ggaatccacc ggtaagattc ggtttctgaa tttaataaga
     6061 cattgcttac cattgaggat ggattcccgc ctgcgcggga atgacggcag acaagcaagt
     6121 ggtcgagatc caacaaaaac gattgaaggt cgtctgaaaa tatcgatttg ataaactaga
     6181 ttttatttca gaagacgtta caagccgata cacaaagaca ttttaaggtc gtctgaaaca
     6241 gcggccgcaa ccgatacgaa aacaaacagg cacaccaaaa atggctattt accaatcagg
     6301 cgtgattttt gaccaagtgg ataccgccaa tcccgattgc tggacattgg acgaatacgt
     6361 caaacgcggc ggctataccg ccctgcgtaa aatcctgtcc gaaaacatct cgcaaaccga
     6421 tgtgattgac gaagtcaaaa cctccggctt gcgcgggcgc ggcggtgcgg gcttcccgac
     6481 cggtttgaaa tggagcttta tgccccgttc tttcccgggc gaaaaatatg tggtttgcaa
     6541 caccgacgaa ggcgagccgg gtacatttaa agaccgcgac atcatcatgt tcaatcctca
     6601 tgccctgatt gaaggcatga tcattgccgg ttacgcgatg ggcgcgaaag ccggctacaa
     6661 ctatatccac ggcgaaattt tcgaaggtta tcaacgcttt gaagccgctt tggagcaggc
     6721 gcgtgccgca ggctttttgg gtaaaaatat tttgggttcg gattttgaat tcgaactctt
     6781 cgcccaccac ggctacggcg catatatttg cggcgaagaa accgcattgc ttgaatcgtt
     6841 ggaaggcaaa aaaggccagc cgcgcttcaa accgccgttc cccgcctcgt tcggcctgta
     6901 cggcaaaccg accaccatca acaataccga aacgttctcc tccgtcccgt ttattatccg
     6961 tgacggcgga caggcgtttg ccgataaagg tattccgaat gcaggcggta ccaaattatt
     7021 ttgtatttcc ggccatgtcg agcgtccggg caactatgaa gtgccgttgg gcacgccgtt
     7081 tgccgaagtc ttgaaaatgg cgggcggtat gcgcggcggt aaaaaactca aagccgttat
     7141 tcccggcggt tcgtccgccc ccgtattgcc tgccgacatt atgatgcaga ccaatatgga
     7201 ctatgactcg atttccaaag ccggctcgat gctcggttcc ggcgcgatta tcgtgatgga
     7261 cgaagacgtg tgcatggtca aagccctcga acgcttaagc tatttctact acgacgaatc
     7321 ctgcggccaa tgcaccccct gccgcgaagg taccggctgg ctttaccgca tcgtccgccg
     7381 catcgtagaa ggcaaaggcc gcatggaaga cttggatttg ctggattctg tcggcaacca
     7441 aatggcaggc cgcaccatct gcgcccttgc cgatgccgcc gtcttccctg tccgcagctt
     7501 taccaagcat ttccgtgatg agtttgcgca ttacatcgaa cacggcgggc cgatgaaaga
     7561 gcataagtgg ggagggtggt aatggtggaa gctaaaattt ttattctata cggtgcagcc
     7621 aacaaaggta agagtacgac actcaatacg ctttttaatc agatttgtcg gaaattttct
     7681 aaatttctag tcttttttga aagatatgga aacggcttag attttgttgc agtatttgat
     7741 catgaaggtc agaggattgg tttttattca tctggtgata atgaatacga ggttaggaga
     7801 aatttataca aactttattc gcataattgt gattttattt tggcacgtca aggacacggg
     7861 gtggtagttg cgatgcagta ggatattgca tggcgatgta aatataatta attggtgtga
     7921 aaagtttgag cctacagatg aagacaatga gcgtgctgtt aaagagttat ttaagtcatt
     7981 taaaaatata ataaatgagt tatagtttta gttggtttta tattggttaa aagcaaaatg
     8041 ctaaaaattt aactttgccg tcattcccgc acaggcggga atccatagtg gaatttacag
     8101 aacccgatat ttgaaaagca gttgccgaaa ttcaaaaaat ggattcccgc ctgtgcggga
     8161 atgacggcaa ggtggcagta aacgttttaa acagtattga ttgtcaatga aactcaaaag
     8221 gccgtctgaa gcccattttt cagacgatct ccataaaaaa ttatttatca aatacccgta
     8281 actaggaacg aaccatgtta caaatcgaaa tcgacggcaa acaggtatct gtggagcagg
     8341 gcgcgacggt aattgaagcc gcgcacaagc tcggtactta tattccgcat ttctgttacc
     8401 acaaaaagct ctctattgcc gccaactgcc gtatgtgtct ggtggatgtg gaaaaagccc
     8461 ccaaaccgct gcctgcctgt gccacgccgg taacggacgg catgattgtg cgtacgcatt
     8521 cggcaaaagc ccgagaggcg caagaaggcg tgatggagtt cctgctcatc aaccatccgc
     8581 ttgattgtcc gacctgcgac cagggcggcg aatgccagtt gcaggatttg gcggtgggct
     8641 acggcaaaac caccagccgt tataccgaag aaaaacgttc cgtcgtcggc aaggacatgg
     8701 gtcctttggt ttccgccgag gaaatgagcc gctgtatcca ctgtacccgc tgcgtacgtt
     8761 tcaccgaaga aatcgccggt ttgcaggaaa ttacgatggt gaatcgtggt gaacactccg
     8821 aaatcatgcc ctttatcggc aaagcggtgg aaaccgagct gtcgggcaac gtcatcgatt
     8881 tgtgtcccgt cggcgcattg accagcaaac cgttccgctt caacgcgcgt acttgggaat
     8941 tgaaccgccg caaatccgtt tccgcacacg acgctttggg cagcaacctg attgtacaaa
     9001 ccaaagacca taccgtccgc cgcgtgttgc cgttggaaaa cgaagccatc aacgaatgct
     9061 ggctgtccga ccgcgaccgt tttgcctacg aaggcctgta tcacgaaagc cgtctgaaaa
     9121 atccgaaaat caaacagggt ggcgagtgga tggacgtgga ttggaaaacc gcgttggaat
     9181 atgtccgcag cgcgattgaa tgtatcgcca aagacggcaa ccaaaaccaa gtcggcgttt
     9241 gggctaatcc gatgaatacg gttgaagagc tgtatctggc caaaaaattt gccgacggct
     9301 tgggtgttaa aaactttgca acccgtttgc gccaacaaga caaacgtctt tcagacggcc
     9361 ttaaaggtgc gcaatggttg ggacaaagca ttgaatcttt ggctgacaac gatgccgtat
     9421 tggtagtcgg tgcgaacttg cgcaaagaac agccgctcct gaccgcccgc ctgcgccgcg
     9481 ccgccaaaga ccgtatggcc ttgagcgtat tggccggcag taaagaagaa ttgtttatgc
     9541 cgcctctgtc tcaagaagcc gcacatcccg acgagtgggc aggccgtctg aaaaacctgt
     9601 ctgccgatgc ggaacatgcc gttaccgcca gcctgaaaaa cgctgaaaaa gcagcggtga
     9661 ttttgggtgc ggaagtgcaa aaccatcctg attacgccgc catttatgcc gccgcgcaag
     9721 agttggcgga cgcgaccggc gcagtgctgg gcattttgcc gcaagccgcc aacagcgtcg
     9781 gtgcggacgt attgggtgtg aactcgggcg agagcgttgc cgaaatggca aacacgccga
     9841 aacaggcagt cttgctgctc aacgtcgagc ctgaaatcga tacggttgac ggtgcaaaag
     9901 ccgtagccgc gttgaaacag gcgaaaagcg tgatggcgtt tacgccgttt gtcagcgaaa
     9961 cgctgctgga cgtgtgcgac gtattgctgc cgattgcgcc gtttaccgaa acatcaggca
    10021 gcttcatcaa tatggaaggc cgtctgcaat ctttccacgg cgtggtacaa ggcttcggcg
    10081 attcacgtcc gttgtggaaa gtgttgcgcg tattgggcaa cctgtttgac ctgaaaggtt
    10141 ttgaatacca cgataccgcc gcgattctga aagacgcgct ggatgcggaa agcctgccgt
    10201 ccaaactgga caaccgcagc acatgggcag gggagggcgt tcagacggcc tcaaaccgcc
    10261 tcgtccgtgt cggcggcgtc ggtatttatc atactgattc tatcgtgcgc cgttccgcac
    10321 cgttgcaaga aaccagccat gccgccgtgc ctgccgcgcg tgtgaatccg aacacattgg
    10381 cacgcttggg cctgcaagac ggacaaaccg ctgtcgccaa acaaaacggc gcaagcgtat
    10441 cggttgccgt caaagccgat gccggtttgc ctgaaaacgt ggtgcatctg ccgctgcata
    10501 ccgaaaatgc cgcgctgggt gcgttgatgg gcactattga actggcggga gcttgattat
    10561 gcaggaatgg ttccaaaacc tctttgccgc aacgctcggt ctgggcgatt tgggcatcac
    10621 cgtaggcttg gtggtatccg tcatcgtcaa aatcgtgatt atcctgattc cgctgattct
    10681 gaccgttgcc tacctgacgt atttcgaacg taaagtcatc ggctttatgc agcttcgcgt
    10741 cggcccgaac gtaaccggcc cgcggggtct gattcagccg tttgccgacg tgttcaaact
    10801 cttgtttaaa gaggtaaccc gtccgaagct gtcaaacaaa gccttattct acatcggccc
    10861 gattatgtcg ctcgccccgt ctttcgcggc gtgggcggtg attccgttta acgaagaatg
    10921 ggtgctgacc aatatcaata tcggcctttt gtacatcctg atgattacct cgctgtcggt
    10981 ttacggcgtg attatcgcgg gctgggcttc caactccaaa tattcgttct tgggtgcaat
    11041 gcgtgcttcc gcgcaaagca tttcctacga gattgccatg agtgccgcgc tggtgtgcgt
    11101 tgtgatggta tcgggcagca tgaacttctc cgacatcgtt gccgcgcagg caaaaggcat
    11161 cgcgggcggt tctgtgttct cgtggaactg gctgccgctg ttcccaatct tcatcgtcta
    11221 tctgatttcc gccgttgccg aaaccaaccg cgcaccgttt gacgtggcag agggcgagtc
    11281 tgaaatcgtt gccggacacc acgtcgaata ctccggcttt gcattcgcgc tgttcttcct
    11341 tgccgaatac attttcatga ttctgattgc cgcgctgaca tcgctgatgt tcctcggcgg
    11401 ctggttgtct ccgttcccgc aaagctgggg cattgtcggt acgccttccg cattctggat
    11461 gttcgtgaaa atggcggcgg tgctgtactg gtatctgtgg attcgtgcaa ccttcccacg
    11521 ctaccgctac gaccaaatca tgcgtttggg ctggaaagtg ctgattccga tcggcttcgc
    11581 ctacatcgtg gttttgggcg tgtggatgat ttcaccgctg aatttgtgga aataagtttc
    11641 agacggcatc ttgaggccgt ctgaacaaag cgattttgaa tacctaacga aatccctgtt
    11701 ttgagggaac ataatatggc taacttagta aaaacctttc tgcttggcga actggtcaaa
    11761 ggcatgggcg taacgctcaa aaactttttt gcccgcaaag acacaattta ttttcccgaa
    11821 gagaaaacgc cgcaatccgt gcgtttccgc ggtctgcacg cgcaacgccg ttatccgaac
    11881 ggagaggaac gctgcatcgc gtgtaaattg tgcgaggcgg tgtgtccggc aatggcgatc
    11941 aacatcgaat cggaagaacg cgaagacggc acccgccgca ccaagcgtta cgacatcgac
    12001 ctgaccaagt gcatcttctg cggtttctgc gaagaggcct gcccgaccga tgcgattgtg
    12061 gaaacccata tttttgaata ccacggcgag aaaaaaggcg acttgcatat gaccaagccc
    12121 atcctcttgg caatcggcga caaatacgaa gccgaaatcg ccaaacgcaa agccgctgac
    12181 gcgccgtacc gctaaggagc agacgaatga ctttttccgt gattctgttc tatatccttg
    12241 ccgccatcgt tttgtacggt gcggttcgta ccgttaccgc caaaaaccct gtccatgccg
    12301 ctttgcatct ggtgctgacc ttctgcgtga gcgcgatgat ttggatgctg atgcaggcgg
    12361 agtttttggg cgtgacgctg gtggtggttt acgtcggcgc cgtaatggtg ttgttcctgt
    12421 tcgtcgtgat gatgctgaat atcgacatcg aggaaatgcg cgccggtttc tggcggcacg
    12481 cgcctgttgc cggtgtggtc ggtacgttgt tggcggttgc cctgatcctg attctggtca
    12541 acccgaaaac cgacctggcc gcatttggtc tgatgaaaga cattccggcc gattacaaca
    12601 atatccgcga tttgggcagc cgtatttata ccgactacct gttgccgttt gaattggcgg
    12661 cggtattgct gctgttgggc atggtggctg cgattgcatt ggttcaccgc aaaaccacca
    12721 atccgaaacg tatggatcca gccgaccaag ttaaagtacg cgccgatcaa ggccgtatgc
    12781 gtctggtgaa aatggaagcg gtcaaaccgc aagtcgaatc tgccgaagaa agcgaagttt
    12841 cagacggcct caagacggaa ggggagggca aagcatgatt actttgacgc attatctggt
    12901 attgggcgct ctcctgttcg gtatcagcgc gatgggcatc tttatgaacc gcaaaaacgt
    12961 gctggtattg ctgatgtcca tcgagctgat gcttttggcg gtgaacttca actttatcgc
    13021 cttctcgcaa catttgggcg atactgccgg acaaattttc gtattcttcg tattgaccgt
    13081 tgccgctgcc gaatccgcca tcggtttggc gattatggtg ctggtgtacc gcaaccgaca
    13141 aacgattaat gttgccgatt tggacgagtt gaaagggtaa tcatgaacga tatgacttta
    13201 tatttgataa ttgcccttgt tccgttggca ggctcgctga ttgcgggttt gttcggcaac
    13261 aaaatcgggc gtgccggcgc gcatacggtt acgatactcg gtgtcgctgt atctgccgtg
    13321 ctgtcggctt atgtgctgtg gggattcctc aatggcagcc gtgccaagtt tgatgaaaac
    13381 gtctatacct ggctgacaat gggcggtttg gatttctccg tcggcttctt ggtcgatacg
    13441 atgacggcga tgatgatggt cgtggtaacg ggcgtgtcgt tgatggtgca tatctatacc
    13501 atcggttata tgcacgatga aaaagtcggc taccaacgct tcttcagcta tatttctttg
    13561 tttacattca gcatgttgat gctgattatg agcaacaact tcatccagct cttcttcggt
    13621 tgggaagcgg tgggcttggt gtcgtatctc ttgatcggtt tctatttcaa acgtccgagc
    13681 gcgacatttg ccaacctgaa agcctttttg atcaaccgtg tcggcgactt cggctttttg
    13741 ctcggtatcg gcttggtgct tgcctatttc ggcggcagcc tgcgttatca ggacgtattc
    13801 gcctatctgc ccaacgtaca aaatgccact atccaactgt tccccggcgt ggaatggtct
    13861 ttgattaccg taacctgttt gctcctgttt gtcggtgcga tgggtaaatc ggctcagttc
    13921 ccgctgcatg tctggctgcc cgattcgatg gaaggcccga cgccgatttc cgcattgatt
    13981 cacgccgcaa cgatggttac ggcgggtctg ttcatggtgt cgcgtatgtc gccgatttac
    14041 gaaatgagca gcactgcgct gtcggtcatt atggttatcg gcgcgattac cgccctgttt
    14101 atgggcttct tgggcgtgat tcaaaacgac atcaagcgcg tggttgcgta ttccaccctg
    14161 tcgcaattgg gctacatgac cgtggctctg ggcgcatccg cctattccgt ggcgatgttc
    14221 cacgtcatga cccacgcctt ctttaaagcc ttgctgttct tggcggcggg cagtgcgatt
    14281 atcggtatgc accacgatca agatatgcgc catatgggca acctgaaaaa atacatgccg
    14341 attacttggc tgaccatgct gatcggtaac ttgtcgctga tcggtacgcc gttcttctca
    14401 ggcttctatt ccaaagattc gattatcgaa gcagccaaat acagcaccct gccgggcagc
    14461 ggctttgcct attttgccgt cctcgccagc gtgtttgtta ctgcgttcta cgcgttccgc
    14521 caatacttta tggtgttcca cggcgaagag aaatggcgca gcctgcctga gcatcatgac
    14581 gatcatcatg gcgaagaaca tcacggcttg ggcaaaaacg acaatccgca cgaaagcccg
    14641 ctggtcgtta ccctgccttt gattctgctt gcaattccgt ccgtcatcat cggctatgtt
    14701 gccatcgagc ctatgcttta cggcgatttc ttcaaagacg tgatttttat caacgccgac
    14761 gcgcatccga ccatgcacat catgaaggaa gagttccacg gcgcattggc aatggtgtcg
    14821 cacagcttga catcgcctgt tttgtatttg gcggctgccg gtgttgccgc cgcgtggctt
    14881 ttgtacgtca aactgccgca cctgccagcg aaaatcgcac agacgttccg tccgatttac
    14941 gttttgtttg aaaacaaata ctacctcgat gccctgtatt tcaacgtttt cgccaaaggc
    15001 acgcgcgcat tgggcacttt cttctggaaa gtcggcgata ccgccattat tgacaacggc
    15061 atcgtcaacg gctccgccaa actggtcggc gcgattgccg ctcaggtacg caaagcccaa
    15121 accggcttta tctacaccta cgccgccgct atggtgttcg gcgtattggt cttgctcggc
    15181 atgaccttct ggggcttgtt cagataaagg cggagtttca gacgacctgt tcatagtttt
    15241 gtagaggtcg tctgaaaagt ttgagaagtg ggcattaagt caaagggtgg aacaaaatgg
    15301 tacccaacag gcgtgaaata gccaacatgc aaattatctg tttcctaaat tttttaggca
    15361 atctcaacaa atatagagga gtcaaaaggg atgacacagt tatttttaca ttcagataca
    15421 ccaaatgaga aggaactgat ttcaatatat gagaaagctg tttcagaggc agtagagctg
    15481 tatattttct ctgcgtatct cactcattgg gatgtaaaaa ataagctcaa tagcaaattg
    15541 caagagttta aatttatttt tggacaagat ttcggattgt ctaaaaaaga ggctattcgg
    15601 aaagtgttga aatggcttcc gtcccatttg aaattcacac tgatggtagc tcaaggcatt
    15661 cagggttttc atcctaaagc tgtattttgg aagaatgata agaatgaata ttatgcttta
    15721 atcggttctt caaatttgac tcatgctgcg tttaatagca attatgaagc aaatattttg
    15781 actaaaattt ccgagcagga ttttataaaa gtcaaatctt gggctgatga aattgcgatg
    15841 aaatctatcc cggtatcgga agattggctt gaggagtatc aggaagctga aattaactat
    15901 aagaaatcaa ctgttagaca atcagtgatg gataagttat ttatggagat gccaaattat
    15961 aatcaagaac tgattgcggc acggagaaag cagatgagaa accatcaaac tgtgtgtaat
    16021 caattaaaaa atcttattaa gcaatgtgca gcaggaaaaa tagacaataa tgatttttat
    16081 ggggaattta ataaattatg gtcttggaaa agcgaaaata aaggagaagg ggtaggcaat
    16141 cgttttcaag ataaaacgtg gaaaaggacg ggtaagtctt cagattttag aaaattatgc
    16201 attgccatcc aatcagtttt tgatgctcca cttactgaga gagataatgt ggttgccaag
    16261 caaattgatt ggctaaaaga atgtggggtt tccactcgtg gttcagtttt ttctgaaatg
    16321 ctttgtcaag aatatccaga tagatatcct gttttaaatg ctcccattaa aaaattttta
    16381 gaagaaaata aatttaagtc tgcaaaagga gcaagcgaag gctccaaata tattgattta
    16441 agtatgaagt taagagcttt actagctatg cagtcagaaa taaaagattt ggctgaatta
    16501 gatgttttag tacaggcaga atatcgcaat cgtacagata ttgattggga ataagtaaag
    16561 aaggggggag agtattcatt ccttctttca gacgacttcc taaaacgttg tctgtaaaga
    16621 ccaacccata caactatatt ttttatttta accacaggtt aaccactatg ttttccaact
    16681 acctactcag cttggcaata tggataccca tcgccgcagg cgtgctggtt ttggcgaccg
    16741 gtaaagacag ccgtgcgccg ctggcgcgtg tgcttgcctt catgggagcg cttgccggtt
    16801 tcttggtaac gctgcccctg tttaccggtt tcgaccgttt gagcggcggc tatcaattta
    16861 ccgagttcca cgagtggatt ccgcttctga aaatcaacta cgcattgggc gtggacggta
    16921 tttcagtgct ctttatcatc ttgaatgcgt ttattacgct gctggtggta ttggccggtt
    16981 gggaagtcat tcagaaacgt ccggcgcagt atatggcggc attcctgatg atgtcgggtt
    17041 tgattaacgg cgcgtttgcc gcgcaggatg cgattctgtt ttatgtgttc ttcgagggta
    17101 tgctgattcc gctgtacctg attatcggtg tatggggcgg cccgcgccgc gtctatgcgt
    17161 cggtcaagct gttcctctat acgctgacgg gttcgctctt gatgttggtt gccatggttt
    17221 acctgtacta tcagacaggc agcttctcta ttgtcgattt ccaaaacatc aaacagattc
    17281 cgttgggcgt acaacagctt ttgtttgtag cgttcttcct gtcatttgcc gtaaaagtgc
    17341 cgatgttccc cgtacacact tggctgccgg atgcccacgt tgaagcgccg accggcggtt
    17401 cgatggtgtt ggcggcaatt acgctgaaac tgggtgcgta tggtttcttg cgctttatcc
    17461 tgccgattat gccggacgcg gctcgctatt ttgcccccgt gatcatcgta ttgagcctga
    17521 ttgccgtaat ttatatcggt atggtggccc tggtgcaaac cgatatgaag aaactggtgg
    17581 cgtattcgtc catcagccat atgggttttg taacgcttgg gatgtttttg tttgttgacg
    17641 ggcagttgga cgactgggcg ttgaaaggcg cggtcattca gatgatttcg cacggcttcg
    17701 tgtcggcggc gatgtttatg tgcatcggtg tgatgtacga ccgcctgcac acgcgcaata
    17761 ttgccgatta cggcggcgtg gtcaatgtga tgcccaagtt tgcggcgttt atgatgttgt
    17821 tcggtatggc gaacgccggc ctgcctgcga cttccggctt cgtgggcgag tttatggtgg
    17881 ttatgggcgc ggttaaagtg aatttctggg tgggcgcgct ggcggctatg acgctgattt
    17941 acggcgcgtc ttataccctg tggatgtaca agcgcgtgat tttcggcgcg attcacaatc
    18001 cgcacgttgc cgaaatgaaa gacatcaatt gccgcgagtt tgcgattctg gcggttttgg
    18061 cggttgctgt tttggggatg ggcctgtatc cgaacgcatt tatcgaagtg gtgcatcagg
    18121 cggcaaacga tttgattgcc catgtggcgc aaagcaagat ttgaggtgtg taaatgaatt
    18181 ggtcagattt gaatttaatg cccgcactgc ccgaagtcgc gctgctgtcg ctgctggtgt
    18241 tattgttgcc ggcggacttg tgggccagtg atgacaaatg ccgttggacg cattacggcg
    18301 cgttggcaac ggtggcggtt acggcggcgg tgcagcttgc cgtgtgggaa cagggcagca
    18361 cgtcttcgtt caacgggatg tatattgcgg acggtatgtc gcgtttggca aaaatggttt
    18421 tatatgcctt gacgtttgtc ctgtttgtct atgccaagcc ctacaaccaa gtgcgcggta
    18481 ttttcaaagg cgagttttac accctgtcgc tgtttgccct cttgggcatg agcgtgatgg
    18541 tgagcgcggg gcatttttta actgcctata tcggtttgga actcttgtcg ctcgcccttt
    18601 acgccctgat tgccctgcgc cgcgattccg gttttgccgc cgaagccgcc ttgaaatatt
    18661 ttgttctggg cgcgctggca tccggcctgc tgctctacgg tatttctatg gtttacggcg
    18721 caaccggttc gctggaattt gccggcgtgc tcgcctcttc cttcaatgaa gaagctaacg
    18781 aatggctgtt gaaactgggt ctggtgttta tcgtcgtcgc cgttgcgttc aaactcggcg
    18841 cggtaccgtt ccatatgtgg atgcccgacg tgtatcacgg cgcgcccact tctgttaccg
    18901 ccttggtcgg cactgccccg aaaatcgccg ccgtcgtttt cgctttccgc atcctcgtta
    18961 ccgggctggg aaccgtgcat catgactggt ctctgatgtt tgccctgctt gccgccgcct
    19021 cgctgctggt cggcaacctt gccgccatca tgcagaccaa tatcaaacgt atgctcgcct
    19081 attccaccgt atcgcatatg ggtttcatcc tgttggcgtt tatggcgggc gcggtcggct
    19141 ttgcggcggg cctctattac gccattacct acgcgctgat ggcggcggca gggttcggcg
    19201 tgttgatggt gttgtcggac ggggacaacg agtgcgaaaa catcagcgat ttggcagggt
    19261 tgaaccaaca ccgcgtatgg cttgcctttt tgatgctgct ggttatgttc tctatggcgg
    19321 gcattccgcc gctgatgggt ttttacgcca aattcggcgt gattatggcg cttttgaaac
    19381 aaggctatgt atggttgtcg gtgtttgccg tcgtgatgtc gctggttggc gcgttctact
    19441 acctgcgcgt ggtcaaagtc atgtattttg acgaatccgg ccgcgcccgt cccgccgccg
    19501 gcggcaacaa tgccgcaaaa tccctcttaa gcgtcaacgc gctgctgttg gtgctgtggg
    19561 gcattatgcc gcaaaccgtt atcgactggt gcgccaaggc gttggagaac acgctgtgag
    19621 ccgccgaaac ggcagccgtg tcagaggctg ccgtttttgt taagatatgc cgttccgcaa
    19681 tgcagttcag acggcatcgc cgccgacaat gcccaaacag aaagcccatc atgaccgcat
    19741 ccatgtacat ccttttggtc ttggcactca tctttgccaa cgcccccttc ctcacgacca
    19801 gactgttcgg cgtggccgcg ctcaagcgca aacatttcgg acaccacctg atcgagctgg
    19861 cggcaggttt cgcgctgacc gcctctcttg cctacatcct cgaatcccgt gcgggagcgg
    19921 tacacaatca gggttgggag ttttacgcca ccgtcgtctg cctgtacctc attttcgcct
    19981 tcccgtgttt cgtgcggcgg tatttttggc acacgcgcaa cagggaataa acaagcaaac
    20041 aagcatagga atgccgtctg aaaccctttc agacggcatt tgtttcattc aagtgcaggc
    20101 cggcatcgct gtgccggcac gtttcagtcg gcgatatacg ccggttttaa tatttgcgtg
    20161 cgactgcaaa ttctgccaac tgccgcaggc gcagggcttt gtcgccgaag ggttcgagca
    20221 gcgcgactgc ttcggcaacc agtttgtgtg cgtatgagcg cgccgcttcc aagcccatca
    20281 gtttcacata agtcggcttg tcgttgtcgg catctttgcc cgccgtcttg cccaaagtcg
    20341 ccgtgtccgc ttcacaatcc aacacatcgt cgatgacttg gaacgccaaa cccagttttg
    20401 ccgcgtaagc gtccaatacg gcaagttccg cgtcggacag atcgggacac gccgtcgccc
    20461 ccaataaaac cgccgcacgg atcaacgccc ccgttttcag gctgtgcatc cgttccaaat
    20521 cggcttgaac catttgtttg ccgacatttg ccaaatcgat tgcctgtccg cccgccatac
    20581 ccgcgctgcc gcccgctttt gccaacaccg acaacattgc caactggcgt gcggcgggca
    20641 gttctgtcgg acggctcaac acgtcgaagg cttgggtttg taaagcgtcg ccggtcagaa
    20701 gggcggtggc ttcgccatat ttgatgtggc aggtcggttt gccgcgccgc aggctgtcgt
    20761 tgtccatcgc cggcatatcg tcgtgaacca aagaatagac gtggatcatt tcgattgccg
    20821 ccattgcctg tccgaccgca tccgccacgg ccccgcccaa ttccgaagcc gccagcacca
    20881 gcatcgggcg cagacgcttg ccgccgtcca aagccgcata gcgcatcgct tcgtgcagcg
    20941 tgtgcgggat ttcgtttcca gacggcaaaa agcgttccag cagcagctct gtctgcgcct
    21001 gcgccctctg ttgccacgct ttcaaatcat tcgtcggatt caaggttcaa ctccttcgtc
    21061 ccgtccgcat ccaaaacctg taatttctgt tcgacttggg ccagcttggt ttggcagtac
    21121 ctgaccagct cgttgccttc ctgataggcg gcaagcgcgt cttccaaggg catttcgccc
    21181 tgcatggact gcgtcagcga ttcaaggcgc gacaaggcct cttcaaacga tttcggggtg
    21241 tttttcttca tagtttcggg tgttccggta aaaagcggat tgtagcattt ttgcaaaacg
    21301 gatgccgtct gaaacccgaa tccggcttca gacggcattt tttccgcccc ggtcggcaag
    21361 gcgttacccg agcagttcgt cggtgatgcc ctgcaaaaag gcgaggcgtt cggggcttgc
    21421 cgccccggtt tcggcagcgg ctttgaaggc gcagccgggt tcggcgcggt gggtgcagtt
    21481 gtggaagcgg cattgcccga caaggtggcg gaaatcgggg aaatagtgcg gcaaatcggc
    21541 ggcttggagg tggtgtaaac caaattcttg caaacccggg gagtcgatga gttgggtttc
    21601 gccgttcaaa tcataaagcc gggcgtgggt ggtggtgtgt tttcccgagt cgagtgcggc
    21661 ggaaatgtcg ccggtgcggg cggtttggct gcctaaaagg gcgttggcca gggtggattt
    21721 gcccataccg ctttgtccga gcaggatgtt gctgtgccct tgcaggacgg ggcgcaggct
    21781 gtcggcgttt tccagcacgc gggtttcgat gacggggtaa cccagcgttt cgtagaattt
    21841 gagtttttcg agccagaggg cggtttcggg caggtcggct ttgttcagga cgatgacggc
    21901 gcggatgccg gcggcttcgg cggcaagcag ggcgcgctgt agcagccgca cgctcggact
    21961 cgggacggcg gcggttacga tgaggagttg ggtaacgttg gcggcgatga gtttggtttt
    22021 ccacgcgtct tggcggtaga gcaggctttg gcgcggtaaa aaatcttcaa tcacaacttg
    22081 ttcggcgttg acggggctga tgcggacgcg gtcgccgcag gcgaaatcga cgcgtttttt
    22141 gcgggtgctg gcttcgtagg ttgtgccgtc gggcgtgcgg acgatgtagc ggcggccgta
    22201 gccggcggta atttgggcgg tgtcgttcat ggtttctttg gggttgggtg tggaaatgcc
    22261 gtctgaaaag gggtgttcgg acggcatcgg ttcagtcgtg ctgccactcg acgtgttcgc
    22321 tgaggaagcc gccgctctgg tgcgcccaga gtttggcgta aagcccgcgt ttttcgagga
    22381 gttcggcgtg tgtgccttct tcgatgatgc ggcctttgtc gaggacgacg agcctgtcca
    22441 tcgcggcaat ggtggagagg cggtgggcga tggcgatgac ggttttgcct tccatcattt
    22501 tgtcgaggct ttcttggatg gcggcttcaa cttcggaatc gagcgcgctg gtggcttcgt
    22561 caagcagcag gatgggtgcg tctttgagca tcacgcgggc gatggcgatg cgctggcgtt
    22621 gcccgccgga gagtttcacg ccgcgttcgc cgacgtgcgc gtcgtagccg cgccgccctt
    22681 tggcatcgga aaggtcgggg atgaagccgg cggcttcggc gcgttcggcg gcggaaacca
    22741 tttcggcatc ggtcgcgtcg gggcggccgt aaataatgtt gtcgcgcacg gaacggtgca
    22801 gcagcgaggt atcttgtgtg accaaaccga tttgggcgcg taaagattct tgggtaacgc
    22861 cgcttatgtc ctgcccgtcg atcgaaaccg tgccgctttg cggttcgtag aaacgcaaaa
    22921 gcaggttgac gatggtggat ttgcccgcgc cgctgcgtcc gatcaagccg actttttcgc
    22981 cgggtttgat attcaggtta aagccgttga gcagcggttt gccggcttcg tagcaaaaat
    23041 cgacgtgttc gaacttgatt gcgccttgcg gcacgttcag cggcagggct tggggcttgt
    23101 cgaggatggt gtgcggtttg gacagggtcg ccatgccgtc gttgacgatg ccgatgtttt
    23161 caaacagccg cgcggattcc cacataatgt attgcgacag cccgttgacg cgcaacgcca
    23221 tcgcggtggc ggtggcgacc gcacccacgc cgacctgtcc gttgtgccaa agccagatgc
    23281 ccagtgcggc ggtggagagg gtcagcgaag tgttgacgat gaagctgcac gaatgcagca
    23341 gagtcgccag ccgcatttgg gcgcgcaccg taaccataaa ttcttccatc gactgcttgg
    23401 catagacggc ttcgcgcgcg ccgtggaaaa agagtttgac ggtggcgata ttggaatagg
    23461 catcggtaat gcggccggtc atcagcgagc gggcattcgc ctgccgtgcg gcggtttgcc
    23521 ccaatctggg aatcagcagg cgcattaccg aagcgaaacc gattatccag ccgataaagg
    23581 gcagcagcag ccatgagtcg agcgaggcga gaatcacgcc cgaagtgatg aaatacaccg
    23641 acacataaac gaccatatcg gcaaccgtca tcaccgcgtc gcgcaacgcc agcgcggtct
    23701 gcatgacttt ggcggacacg cggccggcaa attcgtcctg ataaaaaccg aggctctggt
    23761 tcagcatcag gcggtggaag ttccagcgca ggcgcatagg gaacacgccc tgaagggttt
    23821 gcaggcgcac gttggacgcg gcaaacgccc acacgaccga aaacaccatc atcgccgcca
    23881 ttgccgccag ttcccaacct ttttcggcaa acagttcggc gggcgcgtat ttgccgagcc
    23941 actctacgat tttgcccata aattgaaaaa tcagggcttc cataatgccg atgccggcgg
    24001 tcagcgcagc tagggcggct atccatttcc gcaccccgtc catactgctc cagacaaacc
    24061 ggcacaagcc tttttcgggc gttttcggcg cggcttcggg gtaggggtcg attcgggact
    24121 cgaaccagga aaatattttg ttcaacattg ttttcgattt cggtaaaaca gtttcagacg
    24181 gcatcaaaca caatgccgtc cgaaaggaag gacaataacg ccattttacg ggaaaagccg
    24241 tcgggaagac agcgcggggc ggaaacgcag ggtttcgtca gggcaaacgc cgcgccgcct
    24301 tcggacggca ttatttcagc aggtttttca gggcaaggcg cacgccttcg cccacgtccg
    24361 tccccttcgg aacgcctttg accgccgctt ttgcctcgcg ttcgttgtaa cccagcgcaa
    24421 gcagcgtgct gacgatgtct tccgtttcgt cggcggcggg tgaggcggca aacagcctgt
    24481 ccgttaccgt atgcgcgacc agcttgccgc gcagttccaa gaccatacgt tcggcggttt
    24541 ttttgccgat tcccggagcg gaggagaggc gtttgacatc ttcttctgca accgcccgcg
    24601 ccagctcgtc tgccgtcatc gccgacaaaa tgcccaaagc cgttttcgcg ccgatgccgc
    24661 cgaccttaat cagttggcgg aaggtcttgc gttcttccgc agtggcaaaa ccaaataaaa
    24721 gatgtgcgtc ttcccgaatg ataagctggg taaacagttg tacgctttca cccacgggcg
    24781 gcaggttgta gaaggtctgc atcgatacgt cggcttcata accgacaccg ttgacatcga
    24841 tgacgatttg cggagggttt ttttcaacca gtttgccggt cagtctgctg atcatgtgtg
    24901 ccgaatcctg aagtgtcggg tgcaaaatgc cgtctgaaac cggttcgggc tccagacggc
    24961 acggattgta tcaaattcag tcgtcgcggc gggaggaaat cacgcggccg gtacgggcat
    25021 cgacaacgac tttgtattcc tgtccgtttt tgacgatttc gacatcatag tgcggacggc
    25081 cgtcgtcgtg ttcgagatcg atgtcggtga ttttgccgcc gacacgcgcc caagccgctt
    25141 tttcggcttg ggcgcggctg atgattttgt cttgtttgcc gtgttggtgt gcggcgtgtc
    25201 cgtggtcgtc atcgccgtgt ccgtcgtggt gggcgagtgc gggggcggaa atgcccaaca
    25261 gtgcggttgc ggcggcggtc aagagaaggt gtttgatgtt catatttgcc cttgtaaatc
    25321 aggggttgaa aaatggggat attaataagg tatcgaataa ccgtcaaccg gaggtcagta
    25381 ccgcccgaac cataccgcgt gcctgcgctt cggcttcggc ggcgcgttcc tgcgaggcaa
    25441 acgggcccat tttgacaacg tattcgtaac ggcgtttttc aaccgagagg ttcgggcttg
    25501 atgacgaagc ggcgaaattt tgggcggctt ggttcagata ggcttgtgct tcgtgttccg
    25561 taccgaaaga tttcaagtcg ataaagatgt ctttgttttc ggcaaccggt gcggattggc
    25621 ccgggacgat ttgttcgatt ttgacgtgtg ccgtcccttg gctgacaaag cccaattttt
    25681 gcgcggcggc tttggatacg tcgatgatgc ggttgccgtg gaaggggccg cggtcgttga
    25741 cgcggacgat gacgcttttg ccgtttttgg tgttggttac gcgcacatgg ctggggatgg
    25801 gcagggtttt gtgggcggcg gtaaaggcgt tcatatcgta gcggtctccg ccggaagttt
    25861 tgcgcccgtg aaacctgccg ccgtaccacg aggcgttgcc ggtttgcgtg aattcggcga
    25921 ctttgtagct gcggttggcg gaggcgtgca gtttttccgc cttgaccact gcgtcggcgg
    25981 atgccgtctg aagggagtgt gtgccgaacg cggcggtgag gaggaaaagg gtttttcggg
    26041 ttaaagtcaa aatgtgttcc gttcttgagt tgaagacgaa tgggcattat gccctacgga
    26101 cgcgttccga accgccgcac gctgcggcag cggttcggaa tgtgtccgaa cggttttcag
    26161 acggcatgaa cctgcgttca aacgccgcct gcgtaaccgt gttgccgcca cgcttcaaag
    26221 agaatcacgg agacggtgtt ggaaaggttc atactccggc tgccgggccg catcggcagg
    26281 cggatttttt gcgcggcggg caggctgtcg aggatgtcgg caggcagccc gcgcgtttcc
    26341 ggcccgaaca gtaaaacgtc gcctttttga aacgcggttt catcggggcg cgccgtgcct
    26401 ttggtggtca gggcgaaaat acgtcggcct gccagcgact tgaggcagtc gtcgaagttt
    26461 tcgtgcaccg tcaggctggc gaattcgtgg tagtcgagcc cggcgcgttt cattttggcg
    26521 gaatccaatg ggaagccgag cggtttgaca aggtgcaaat ccgcgccggt attggcgcac
    26581 aggcggatga tgttgcccgt gttcgggggg atttccggct gatataaaac gatggtaagc
    26641 ataaatatca atcacttata ggcgcgtaac cttgccataa ggcagatggg gtgtcaaaaa
    26701 atttagttat tttttcattg gcgtgcgtgc cagcgtccag cagcagattc ggtttgcgcc
    26761 cgattttttc agcgtctttg ccaattcgtc cagcgtcgcg ccggtggtaa agacatcgtc
    26821 gattaacaaa atattacagt tttccggtat cggtgtgcgg atttcaaagg cgtttttgat
    26881 gtttcgccgc cgttcgccgc ctttgagcgt gctttgcggc gggcggtggt gtcggaatac
    26941 ggtgtgtcgg ggcagtatct gccagccgta gcgttgcgcc aacaacccga cgatgctctc
    27001 gctttggttg aacccgcgtt gcagcagccg ctccctgctt agcggaacgg gtaggacgaa
    27061 atcgaaacat tcgtctgaaa gccggtcggg cggattctgc atcgtcaggt ctgccagcgg
    27121 ctgcgccatg cccaaatcag ccaagtgctt cagcgcgcgt atcatattgc tgacgggcgg
    27181 ttcgtaatgc agcgatgccc acatccggtc gaatgcgggc ggttttttct gacatccgcc
    27241 gcacaccgcg ccgccttgaa cgtgcctgaa acacaggggg cagctgtttg ccggcgtcgg
    27301 tgcggtatgc cgccaaatcg gtattgcagc cggcgcagat gccgtctgaa acgccggacg
    27361 aaccgtggca taatacgcaa cgcctgatag tgggcgcgtc tgcgatgcgc cgccaacaag
    27421 agagaaaatt catgcctgat gccgtcaaaa aagtttacct gatacacggt tggggggcga
    27481 accgccacgc gttcgacgat ttgatgccgc gcctgcccgc aacgtggccg gtgtccgccg
    27541 tcgatttgcc cggacacggg gacgcgccct tcgcgcaacc cttcgatatt gaggcagctg
    27601 ccgacggcat tgccgctcaa atcgatacgt cggccgacat tctcggctgg tcactcggcg
    27661 gattggtcgc gctgtatctg gcggcgcgcc atcccgacaa agtccgttcg ctctgcctga
    27721 cggcgagttt cgcacggctg acggctgccg aagactatcc cgaagggctt gccgcgcctg
    27781 cactgggcaa aatggtcggc gcgttccgaa cggattatgc caaacatatc aaacagtttc
    27841 tacaactcca gcttctgcac acgcctgatg ccgccgaaat catcggcaga atcctgcccg
    27901 atttggcgcg ctgcggcacg cctcaagcct tacaggaagc tttggacgcg gcggaaaggg
    27961 cggatgcgcg gcatttgttg gacaagatag atgttccggt actgctggtg ttcggcggca
    28021 aagacgcgat tacaccgccg cgtatgggcg agtatctgca tcggcatttg aaaggaagca
    28081 ggctggttgt gatggaaaag gcggcacacg cgccgttttt gagccatgcg gaagcgtttg
    28141 ccgcgctgtg ccgcgacttt gtcgaagggg gtttgacatg aatccgcagg acaaatgctg
    28201 gcaggttcac cgccatcttg ccgaacatac cgaccaacgc ctgacactcg tccgcaacgt
    28261 gcccgggcat atcctgcttg ccggtgcgga tgcggacatc agccgcagcc tgttggcgaa
    28321 gcgctatccg ccggcggtat ttgaagaata cgattcccgt gcggattttt tggcggctgc
    28381 cgcagccgtg cgcaagggcg gtttctggca aaaactgacg ggcaggggcg tggtgcagca
    28441 ctgccaatcc ccgaccgtgc cgctgcccga agcgtgtgcc gatatgttgt ggtcgaacct
    28501 cggactgttg gcggcggaac aaatccttcc cgtgctgcac aactgggcgc gcgcgctgaa
    28561 gacggacggg ctgctgtttt tcacctgctt cgggcgcgat accttggcgg aactgaaatc
    28621 ccgtctgaaa gaaaacggca ttgaaagccg cagcgtgatg tttcccgata tgcacgactt
    28681 gggcgatatg ctcgccgaaa acggctttta cgatccagtt accgatacgg cgaagctggt
    28741 gttggactac aaaaaggcgg aaacgttttg ggcggatatg gacacgctgg gcgtttggcg
    28801 ggcggtggcg tgggacgacg aaaacgccgc gcgttcgtgt gtcggggcga tatttgaacg
    28861 ggaaggcggt ttgggcatta cgctggaaac ggtgtacgga cacgccgtga aaaaactgat
    28921 gctgccgcag ggggagaacg tggtgcgttt ttttccgaag agatgatgtg cagatgacgt
    28981 ctgaagccgt ttccaagttt cagacggcat ttgtctgtga aaaccgacag aaataaagga
    29041 aatgccgatg tatagtgaat taaatttaaa ccggtacggc gttggctcgc cttgccgtac
    29101 tatctgtact gtccgcggct tcgccgcctt gtcctgattt aaatttaatc cactatgtgc
    29161 cgatgccgga gacgtatatt gcgtctataa catcagactg aagcagtaca ctgcctgcca
    29221 ggttacccga gttgaagaac acggtggcaa aaaaacacat gcgaccctgc tggctttgga
    29281 ctggcggggc aacaaaccgc ttggggcggc ggagctggcg gatttgaaac cgctttacaa
    29341 agactttatg tattgggaac gcggtctgca catgtataag gcaagtgccg tcgtgccgac
    29401 gggatatgta cgggttggga ataccgcgcc gctgtgcggc gaagacacgc agcggtatgc
    29461 ctctttttgg ggcgacggct acgacgtgta ccgtcagttg agatggcggc agatacccga
    29521 aaaacagaga aaggcattca aaaaagccgc caaaagcaaa aataccgtga tgtttgccgg
    29581 acgggaatac ggcatatcca aacagaattt gagcgatgtt tgggatgatt ttgaagacgc
    29641 gatggaactg aaggcgtttc cctgcctgtc ttcgctgttt ctgaccaagt ggcataaaaa
    29701 tctatatgaa tatctggagg aatatccgtt tatcacccga ttgtgtctgg aaaaccacgg
    29761 tcagaccgtg ctggatttca gcaatacgcg cataaccgac ctttctgtcg atatgaccgg
    29821 cgtggaatcg ctttatctga acgaaggctt ggacagcctg aacctgaaag gcgaaatcaa
    29881 ggaaaactgc aaggtctgta ccgccggaaa gggcgcgggt ctgattttgg aagtgggcaa
    29941 aagcgttccc aaagtgcggg gtttggaaaa cctgaccgcc gtcaacgtca tgggcattgc
    30001 cgatttcgat atgcaaaacc tgtcggaaac gtatcccaag ctcaaaacca tacggctgtg
    30061 gggcaaaccg ggcaatatcg ccaatttttc cgccgtatcg ggatttgagg atttggaagt
    30121 gttcactgcc gtagatttgt tcggtttcgg cgcggacgac atccctcatc ccgaccggct
    30181 gccgaagctg cacaggctgt ggatgagcag cctgcccgaa gaggcggcga aggcagtgaa
    30241 gaaactttat aagaaacgga aggaagacgg gctggacccg tggatagaaa aagcccgaaa
    30301 acccgaatgg ctggcgcaaa atttcgacaa ccccttccgc gattgggacg gcgcggagca
    30361 tatcccgaaa agccatgcca aaaaagcggc ggagctgtac cggaaaaccc gtgccggcgt
    30421 ggtcaaactg ctcggcaatc cgcctgaaaa cacaggggag gggctggcag aggcggtcaa
    30481 agcctatacc ggaggtttca acaaaatgga caaaaaacac tttatcgaca cggtagagcg
    30541 ggaagatatt gccgaggcgt tggaaacgat attggacctg ataccggatg ggtcttgtgc
    30601 cgacaaggaa aaactgtttg aaatatttga taaaaacagg aatttttaaa cagaggcaat
    30661 gccgtctgaa acttggtaac gggcttcaga cggcatcccg ttccaacacc gccaacaccg
    30721 ccgcaccgta acgtgcggct ttttcttcgc ctacgccgta tacggcggca agctccgcca
    30781 aacctttcgg ctgtttggcg gcaatggcgc gcagggcggc tttgctgaga atgcggtagg
    30841 gttcggattg ttcgtgtttt gcggtttcgt tgcaccattg aatcagggcg cgcatcaggc
    30901 agcgtttgtg tttggcggtt tcatcgatgc cgtctgaaaa cggacggcag acggcgagga
    30961 tgtcccgtcc gtatttggcg gcgcgtacgc tgcccaagcc gtgtacggct tcgaggtcgg
    31021 tttcggtttc gggcgtggcg gcaagcatat cggcaaggct ttcgtcggag aggacggtat
    31081 gcagggcgca gttttccgcc cttgcctgtt cataccgcca ggcttcgagt ttttgacgca
    31141 gttgttgttc gcgttcggtt tgcggacgga tgacggcatc gtggctaaag tcggcagcgt
    31201 tttggcagac tttgagtatg ccgtgtccga aacgttcggt tttggcttcg cccaagccgt
    31261 agatgtcgtg cagcccgttg aggttttgcg gcattttttc gacaaggtcg cgcagggttt
    31321 tgtcgccgaa aatcatatag gcggggatgc cttcggcttc agcctgtttc atgcgccaaa
    31381 cgcgcaatgc ctgccacagg cgttcttcgc gttcggtacg cagccagttg tctttgaggg
    31441 tgcgggtggc gggcttgtcg cgcttgagcg ggcgcagcat cacttcggtt tcgcctttga
    31501 ggactttttt ggcggcttcg gtcagttgca atgcctgata tcgggcaatg ttgacggtga
    31561 ggtagccgag gctgatgcac tggcggatga cgctgcgcca ttctttgtcg gacaattccg
    31621 cgccgatgcc gaatgtggac agttgctcgt gccggttgcc gcgtatccaa tcgtcgcttt
    31681 tgccgcgcaa aaggttggtg atgtaaccgg cggcaaagcg ttgtccggcg cggtacacgc
    31741 agctgagtaa tttttgcacc aacaccgtgc cgtcaaaccg tacgggcgga tgcaggcagt
    31801 tgtcacaatg tccgcagggt tcggatgctt cgccgaaatg tttgagcagc agtacgcggc
    31861 ggcaggcggc ggtttcgcag acggaaagca tggcatcgag tttttgcatt tcgatttgct
    31921 tttgcacctc gccgctgttg ccttcggcaa tccgttcgcg cagcaacacc caatcgttca
    31981 agccgtaaca cagccaactc acggcaggca gcccgtcccg gccggcgcgc cctgattcct
    32041 gatagaaatg ttcgacactc tggggcatat cgagatgggc gacaaagcgc acgtcgggtt
    32101 tgtctatgcc catgccgaac gccacggtcg ccaccacgat aatattgtct tcatgcgtaa
    32161 agcggcgttg gttttcctcg cgcacgtcca tgctcaaacc ggcatgatac ggaatcgcgt
    32221 ttaatccgtt ttcacgcaaa aactgcgccg catcttcaac ctttttgcgg cttaggcaat
    32281 acacaatgcc gctttgcccc gtcatttctt tgcggatgaa atccagcaat tgttttttgc
    32341 cgttgttttt ttcgataacc tgataataaa tattcgtgcg gtcaaagctg gagacaaatt
    32401 cggacgcgtc gtccaagtgc agataatgct tgatgtcggc gcgcgtggcg gcatcggcgg
    32461 tggcggtcag ggcgatgcgc gggatgttcg gatagcgttc ggcaagcata ccgagctgtt
    32521 gatattcggg gcggaaatcg tgtccccacc ggctgacgca gtgcgcttcg tcaatggcga
    32581 acaggctgac ggtttgttgg tcgagaaaac gtaaaaagcg gtcggtaacc aagcgttccg
    32641 gcgcgacata aagcagcttc agacggcctt gggcaagctt gtcggcaatc tcgcgcgcct
    32701 catctgccga tgtgccgctg ttgactgccg ccgcttcaat cccggccaca tgcaggctgg
    32761 ccacttggtc gttcatcagc gcaatcagcg gcgatacgac aaccgccacg ccttcgcgca
    32821 tcagcgccgg aatctggtaa cacaaagact tgcccccgcc cgtcggcatc agcaccgtca
    32881 aactgccgcc gcctgccaaa gtattgatga catcctcctg cctgccgcga aattcgggat
    32941 agccgaatac ttcgtgcaaa atctgtctgg cggtgggtcg gtgcgtcatt gttccgtgtc
    33001 cggtaagggt gttggtcggt cggcggcaat atgccgtctg aaatcgggat tttagaagag
    33061 tttgcccact tccgccccaa tatcgtcggc acgcataaac gtttcgccga tcaggaaggt
    33121 atgcacgccg cgcgcccgca taaattccac atccgccttg cctgtaatgc cgctttcggt
    33181 aacgacggtt ttgccttcca gcgcgggcag cagcgacagg gtttggtcga gggagacttc
    33241 aaaagtccgc aggttgcggt tgtttacgcc ccacaacggc gtggtcaggt tgcggcattt
    33301 ttccaattcg gtttcgtcgt gcagctcgag caggacggtc atgcccaatt cgtgcgccag
    33361 cgcctcaaag cgttccaatt gtccctgttc cagtgcggcg gcaatcagca ggacggcatc
    33421 cgccccccat gcgcgcgcct gataaacctg gtattcgtcg atgatgaagt ctttgcgcag
    33481 cacgggcagc aatacggctt cgcgcgcctg tttgaggtat tcgggcgaac cttggaaata
    33541 gggttcgtcg gtcagtaccg acaaacacgc cgccccggcg ttttcatagg cgcgggcaat
    33601 ctcggcaggg cggaagtcgg ggcggattaa ccctttgctc gggcttgcct ttttgatttc
    33661 ggcaatgacg gcgggcaggt ttaggcggtg tttgccgcgt atcgaatcga tgaagctgcg
    33721 gacgggcgcg gcttctgcgg caagtgcgcg gatgtgttcg gcgttgacgg cggctttttg
    33781 ggcggcaact tcctgtgcct tggtggcaag gattttattg aggatgtcgg tcatgtcgtg
    33841 ttccgtattt gtccggggaa agggggaata ttagcatcaa accgttaacg cctgtttgtg
    33901 cggaagctgt caaaatagga caggacggtc tgcggcagcc attgcaggtg cagcctgccg
    33961 ccggtgctgc tgacaaagcc ggcgtgcccg ccgtgtgcag gttggaacag ggtaacggct
    34021 tcggacgctt cgtccgcacg gggcagggct tcgggcggca ggaaggggtc gttggcggca
    34081 ttgagcagga gcagcggttt ggcaacgtgt ttgagcagcg gtttgcagga agtttggcgg
    34141 tagtagtcgt gccggtcggc aaagccgtgc agcggtgcgg tgaaacggtc gtcaaactcg
    34201 cccagtgttt tgcaccctgc ggcaaatgcc gtctgaaaac cttggagcga acgtgctttg
    34261 ggtatcagtg tgcggaggaa gtagcgcgtg tagagcagcc gcgtgatgcc gctgtcgaag
    34321 cggctgcctg ccgcctctgc atcaacgggg gcggatacgg cggccgaggc gtgcggcaat
    34381 gcctttttgc cctgttcgcc caaatatttt gccggcgcgt tgccgcccag cgatacgccg
    34441 acggcgtata tttcacggta acgcgcggtg agcgtgtcca aagcaaaggc gatttcggcg
    34501 gtatcaccca agtggtagaa caccggggcg gtgttcgcta cgccgccgca gctgcggaaa
    34561 tggacgactg cgccgtgcca accccgattg cgtaccgcga gcatcagttc gaccgcgtaa
    34621 tggctgcggc tgcttccttc caaaccgtga aacagcacga ccagcggcgc atcgggcgaa
    34681 atgccgcctg aaaagtcgta ggcggttttg gttttacccg tgctgtcggg aagcatctcg
    34741 cggcggtatg cgggtgcggg gtgttgcagg aatttggcgg caatcgtgtc ggcattgccg
    34801 ttgcggagga aaaagggcgt gtccggcggt gtcaaaatca taaggtatcg gtttttctgt
    34861 tttcagacgg cattggtgat gcggcagccc gtccggccgg tgcggacgcg ggcgatgcgc
    34921 gcccggatat aggcgcggaa aaagcgtttt ccgaaaaagg atatcggcat cggtcagctt
    34981 tccacgcgtt tgaaatggcg cggacggaag cccaaagccg ccagagatgc gaaatacagt
    35041 ccgccgccga cggcaatcag gatgcagagc tgccccgctt tccgcattcc gccggcgtgc
    35101 gcccattcga acggcaggca agcctgcgcc gcccacagtc cgccgcacat cacggcgagc
    35161 gcgagcagca ttttcgccaa gaacgccgcc caacccctgc cgggccggta aataccgtgt
    35221 ttgcgcaaca ggaagaacaa caatccggcg ttgatgcacg cgcccaggcc gatggcgagc
    35281 gaaagcccgg cgtgtttcaa cggaccgata aaggcgaggt tcatcaactg cgtgcagatg
    35341 agcgtgaaga tggcgatttt gacgggcgtt ttgatgtttt gccgcgcata aaagccggat
    35401 gccaacactt taatcataat taaaccgatt aaaccgaaag aataggcaat cagcgcgtgt
    35461 tgcgtcattt gtgcgtcaaa cagcgtgaat tctcggtaca taaacagcgt cgccaccagc
    35521 gggaacgaca ataccgccag tccggccgcc gccggcagcg tcagcagcat gcacaggcgc
    35581 aaaccccagt cgagcagggc ggaaaactgt tccgtatctt ggtttgccga gtgtttggac
    35641 aaagtcggca gcaaaattgt accgagtgca gcccccagca cgccgccggg cagctccatc
    35701 atgcggtcgg cgtaatacat ccatgaaacg ctgcccgatt gcagataaga cgcgaaaatc
    35761 gtgttgataa ccaaagaaat ttgcgccacg ctcacgccca aaatcgcagg cgccatctgt
    35821 ttcatgacgc ggttgaccgc cgcatctttg aaattcagtt tgggcagttt caaaaagccc
    35881 agtttcgcca gccacggcag ttggaaaccg agctgcaaaa taccgccgac aaaaaccgcc
    35941 cacgccagcg cggtaacggg cggatcgaaa tacggcacga aaaacagtgc gaatacgata
    36001 aaagagatgt ttaaaaacgt gggcgtaaac gcgggaatgc cgaacttatg gtaggaattg
    36061 agtatcgagc cgacaaaaga agacaaagaa atcaataata tataaggaaa cgtaatccgc
    36121 agcaggctga tggaaagttg gaacttgtcc gcgtctttgg taaagccggg cgcggaaaca
    36181 taaatcaccc aaggcgcggc aagtatgccc agcgcggtaa cgacgatcag cacaaacgac
    36241 agcattcccg caacgtggcg gataaaagcc tccgtcgcct ctttagaacg cgtttcctta
    36301 tattccgcca aaatcggcac aaacgcttgg gcaaacgccc cctccgcaaa cacgcggcga
    36361 agcaggttgg gcagtttgaa cgcgacaaaa aacgcatccg tcgccatacc cgcgccgaat
    36421 gcccgcgcaa tgaccgtatc gcgcacaaat cccaaaacgc gcgacaccat cgtcaggctg
    36481 ccgacttttg ccaaagctcc aagcatattc atcgtgtttc cttttcggct gaaaccccgc
    36541 cctttagggc ggcaggatca gactttatag tggattaaca aaaatcagga caaggcggcg
    36601 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg gtttttgtta
    36661 atccactata tttgaaaggg atgtaaccct tccaaatcag ggcaacgcat agggcggtgc
    36721 tttatgtgcc gtcctgtgtg ttgaaacatc attgttcccc aacagccgta tccgtccggg
    36781 gcggcgcgta ttgtacgaca gaaaccgctt cggatggcat cgggtttgat gccgtctgaa
    36841 gcggtttccc gaaacgaaaa cgtccttttt cggcggcaaa ctgtatcaac acgcgaaaat
    36901 acaataaaat accaatatcc gatttatttc caacatcacg gagagccgta tgaaatccag
    36961 acacctcgcc ctcggcgttg ccgccctgtt cgcccttgcc gcgtgcgaca gcaaagtcca
    37021 aaccagcgtc cccgccgaca gcgcgcctgc cgcttcggca gccgccgccc cggcaggact
    37081 ggtcgaaggg caaaactaca ccgtccttgc caacccgatt ccccaacagc aggcaggcaa
    37141 ggttgaagtg cttgagtttt tcggctattt ttgtccgcac tgcgcccgcc tcgaacctgt
    37201 tttgagcaaa cacgccaagt cttttaaaga cgatatgtac ctgcgtaccg aacacgtcgt
    37261 ctggcagaaa gaaatgctgc cgctggcacg cctcgccgcc gccgtcgata tggctgccgc
    37321 cgaaagcaaa gatgtggcga acagccatat tttcgatgcg atggtcaacc aaaaaatcaa
    37381 gctgcaagag ccggaagtcc tcaaaaaatg gctgggcgaa caaaccgcct ttgacggcaa
    37441 aaaagtcctt gccgcctacg aatcccccga aagtcaggcg cgcgccggca aaatgcagga
    37501 gctgaccgaa accttccaaa tcgacggtac gcccacggtt atcgtcggcg gcaaatataa
    37561 agtcgaattt gccgactggg agtccggtat gaacaccatc gaccttttgg cggacaaagt
    37621 acgtgaagaa caaaaagccg cgcagtaggc ccgtttgaaa aatgccgtct gaaacttggt
    37681 tttcagacgg cattttgatt gggtttagaa cgtaaagccc gtttccagtt cttcatcgcc
    37741 gaccagttcg accaagagcg cgtagagcgg ggcgagttcg gcataacggc gcgatacgcg
    37801 gcgcagatag tttaagaaac gcgggatttc cggacggtat ttgtctttgc cgtcgcggta
    37861 gtacaggcgt gcgaagatgc ccgcgacctt caagtgccgc tgcacgccca tccattcgaa
    37921 ccggcggtaa aactcgtcaa actcggcagg gacaggcaag ccggcagccc gcgccttttc
    37981 ccagtagcgg ataaccaaat ccaaaacaaa ctcttcttcc cactcgataa aggcatcgcg
    38041 caacagcgac accaaatcgt aggaaatcgg gccgtaaagc gcgtcttgga agtctaaaac
    38101 gcccggcctg ccgcgcgtca gcatcaggtt gcggacgata aagtcgcggt gcacgtagac
    38161 tttgggctgc gccaacaagg gcggcagcag cgtatcggcg gtttgctgcc aaagttggcg
    38221 ttgtttgaat gttaattcgc gccccaattc ttttgcgaca aaccattccg ggaacaggtt
    38281 gatttcgcgc agcatcgttt cacggtcata ttcgggcaaa accccttcac ggcttgcctt
    38341 ctgcaatccg accagctcgc cgatggcctc cagcaacagg gctttgtgtg ccgcttcgcc
    38401 ctgctcctga agcatcgcgg tcaaaaacgt cgtattgccc aagtcgttca ataccacaaa
    38461 ccccaaatcc gtgtccgcgt gcaatacctg cggcacattg accatgtcaa acagcttctg
    38521 caccttcaaa taaggcgcga cactcatctt gtcgggcggc gcatccatac agacgacgct
    38581 gccgccgtct gaaaacgccg cacggaaata gcggcggaaa tcagcatccg ccgccgcgaa
    38641 ggacagatcg aagtcccgtt cgggataaac ggtctgaagc caatttttca gttcggtttg
    38701 ccgttgcata acagtatccg ggcatttcgg gttacaataa acactattct aaccggtaaa
    38761 ccgacttgag gggcgatttt ggctcgttta ttttcactca aaccactggt gctggcattg
    38821 ggcttctgtt tcggcacgca ttgcgccgcc gataccgttg cggcggaaga ggcggacggg
    38881 cgtgtcgcag aaggcggtgc gcagggcgcg tccgaatccg cacaagcttc cgatttgacc
    38941 ctcggttcga cctgcctgtt ttgcagtaac gaaagcggca gccccgagag aaccgaagcc
    39001 gccgtccaag gcagcggcga agcatccgtc cccgaagact atacgcgcat tgttgccgac
    39061 aggatggaag gacagtcgaa ggttaaggtg cgcgcggaag gaagcgttat catcgaacgg
    39121 gacggcgcag tcctcaatac cgattgggcg gattacgacc agtcgggcga caccgttacc
    39181 gtaggcgacc ggttcgccct ccaacaggac ggtacgctga ttcggggcga aaccctgacc
    39241 tacaatctcg atcagcagac cggcgaagcg cacaacgtcc gtatggaaac cgaacaaggc
    39301 ggacggcggc tgcaaagcgt cagccgcacc gccgaaatgt tgggcgaagg gcgttacaaa
    39361 ctgacggaaa cccaattcaa cacctgttcc gccggagatg ccggctggta tgtcaaggcc
    39421 gcctctgtcg aagccgatcg gggaaaaggc ataggcgttg ccaaacacgc cgccttcgtg
    39481 ttcggcggcg tccccctttt ctatacgcct tgggcggact tcccgcttga cggcaaccgc
    39541 aaaagcggac tgctcgtccc gtccgtatct gccggttcgg acggcgtttc cctttccgtc
    39601 ccctattatt tcaaccttgc ccccaacttc gatgccactt tcgcccccgg cattatcggc
    39661 gaacgcggcg cgacgtttga cggacaaatc cgttacctgc gtcccgatta cagcggacag
    39721 accgacctga cctggctgcc gcacgataag aaaagcggca ggaacaaccg ctatcaggca
    39781 aaatggcagc accggcacga catttccgac acgcttcagg cgggtgtcga tttcaaccaa
    39841 gtctccgaca gcggctacta ccgcgacttt tacggcggcg aagaaatcgc cggcaacgtc
    39901 aacctcaacc gccgcgtatg gctggattat ggcggcaggg cggcgggagg cagcctgaat
    39961 gccggccttt cggttcagaa ataccagacg ctggcaaacc aaagcggcta caaagacgaa
    40021 ccttacgcca tcatgccccg cctttctgcc gattggcata aaaacgcagg cagggcgcaa
    40081 atcggcgtgt ccgcacaatt tacccgcttc agccacgacg gccgccaaga cggcagccga
    40141 ctggtcgtgt atcccggtat caaatgggat ttcagcaaca gctggggcta cgtccgcccc
    40201 aaactcgggc tgcacgccac ttattacagc ctcgacagtt tcggcggcaa agcatcccgc
    40261 agcgtcgggc gcgttttgcc cgttgtcaat atcgacggcg gcacaacctt cgaacgcaat
    40321 acgcgcctgt tcggcggcgg agtcgtgcaa accatcgagc cgcgcctgtt ctacaactat
    40381 attcctgcca aatttcaaaa cgacctgccc aatttcgatt cgtcggaaag cagcttcggc
    40441 tacgggcagc ttttccgcga aaacctttat tacggcaacg accgcatcaa cgccgccaac
    40501 agcctttcca ccgccgtgca gagccgtatt ttggacggcg cgacggggga ggagcgtttc
    40561 cgcgccggta tcggtcagaa attctatttc aaggatgatg cggtgatgct tgacggcagc
    40621 gtcggcaaaa atccgcgcag ccgttccgac tgggtggcat tcgcctccgg cggcataggc
    40681 gggcgtttca ccctcgacag cagcatccac tacaaccaaa acgacaaacg cgccgaacat
    40741 tacgccgtcg gcgcaggcta ccgccccgcc cccggaaaag tgttgaacgc ccgctacaaa
    40801 tacgggcgca acgaaaaaat ctacctgcag gcggacggtt cctattttta cgacaaactc
    40861 agccagctcg acctgtccgc acaatggccg ctgacgcgca acctgtctgc cgtcgtccgc
    40921 tacaactacg gttttgaagc caaaaaaccg atagaaatgc ttgccggtgc agaatacaaa
    40981 agcagttgcg gctgctgggg cgcgggcgtg tacgcccaac gctacgttac cggcgaaaac
    41041 acctacaaaa acgccgtctt tttttcactt cagttgaaag acctcagcag cgtcggcaga
    41101 aaccccgcag gcaggatgga tgtcgccgtt cccggctaca tccccgccca ctctctttcc
    41161 gccggacgca acaaacggcc ctgaccgtcg gaaacccggc aggagcaccg ttcctgcaca
    41221 agatagcatt ccaccgacaa ccccaaaccc gccatcaaag gcaggattca aacgataagg
    41281 aaagaatgat gaaaatcaaa gccctgatga ttgccgccgc attgctggca gcagccgatg
    41341 tccacgccgc accgcaaaag gcaaaaaccg cgcccgccaa agcggtcaaa gctgccgcca
    41401 cggcgcaaaa agaagccgca cccgcacaac agcagggcgg tatccgcttt tcagacggca
    41461 ttgccgccgt tgccgacaac gaagtcatca ccaaccgccg gcttgccgaa gccgttgccg
    41521 aagccaaagc caccctgccc gaagacgcgc agataagtga atccgagctg tcccgacagg
    41581 tgctgatgca gcttgtcaac caatccctga ttgtacaggc gggcaaacgc cgcaacattc
    41641 aggcaagtga agcggaaatc gatgccgtcg tcgcacaaaa tcccgccctc aaaaacctca
    41701 gccccaccca acgccgcgag cttgccgaca acatcattgc cgaaaaagtc cgccagcagg
    41761 cagtgatgca aaacagccgc gtgagcgaag ctgaaattga tgccttcctc gagcaggcgc
    41821 aaaaacaagg catcaccctg cccgaaggcg caccgttgcg ccaataccgc gcccaacaca
    41881 tcctgattaa agccgacagc gaaaacgccg ccgtcggcgc ggaaagtacc atccgcaaaa
    41941 tctacgacca ggcgcgcaac ggcacggatt tcgcaggttt ggcacgccga tattcccaag
    42001 acgcaagcgc gggcaatggc ggcgacttgg gctggtttgc cgacggcgta atggttcccg
    42061 cctttgaaga agccgtccac gcgctcaaac ccggacaggt cggcgcgcct gtccgcaccc
    42121 aattcggctg gcacatcatc aaattgaacg aagtgcgcga tgccggcacg ccggaggaac
    42181 gtatccgcaa ttccgtgcgg caatacatct tccaacaaaa agccggacag gcaaccgtca
    42241 acctgttgcg cgacctgcat tccggcgcgt atgtcgacat ccgctaaggc ggtttgaatc
    42301 caaaagtcat accgatcggg caaaagtccg ggcggtatgg cttttttgaa tttcgagtta
    42361 cttttacacc gtcattcgtc attcccgcgc aggcgggaat ctagcaacga aaagtaacag
    42421 gaatttatcg ggaatggccg gagtttaaag gtctggattc ccgccttcgc gggaatgacg
    42481 attcagaagt tacacgaaac tcaaaaaaaa acgaaaccga acgaaccgga ttcccgcttt
    42541 tacgggaatg acggcatatc ggtttccgtg aggacaaacc tagattcccg cctgcgcggg
    42601 aatgacggga ttttagattg cgggtattta tcgggaatgg cgatttaaaa attacctaaa
    42661 actaaactta aaagcaactg aaacttaaaa aactaaaatt tcgcctgcgt gggaaggatg
    42721 ggatttcaga tttctgtttt tgtgggaatt tgccgtatcg ggcttcagac ggcattgcct
    42781 gccgttttgc ccccgggtgc ggctgccttg atgtagttga gcgagacaaa ctgcttctcg
    42841 gcatccaatt cggtgatttt aaacaatgcc tgcgatttgg gcagcgcgtc aaacggaata
    42901 ccggtcgcgc gcgttgtcag tggcagacct tcgatgcgga cgaggtcttc tttcagaatc
    42961 gtcgcggtca gttcgctgat gccttgttgt tgcaggtaca cgaggctcca gtaggcttcc
    43021 atctgccgtt ggaagtcggc gtaggcggcg taggcggtgt cgaaatcgcg cagggcggcg
    43081 aagagcgcgg catcgctttg ctggaagagc ggctcggcgg tgtcgtctat caggctgagg
    43141 agttgttttt ggttgatgta gtcggcggcg cggcgcagcg gcgaggtaaa ccagccgtaa
    43201 tgctgcacgc ccatgccgat gtgcggctcg gattgggtgc tcatgcgcac tttgcctgcc
    43261 ggttggacgc ggaaaaggcc gggcagcccg ttttcgtcta gcatttgcgc ccaagtgctg
    43321 ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg gcgaaccgcg ttcgcggcgg
    43381 acgacggaga cgttgccttc ttcatccaat tcgatgctgt aatcgtattg cggcgcgcgg
    43441 tcgggttcgt atttgccgcg cgctttttgc agggcggtgg cgaattgata gaaccaaatc
    43501 aggtcttgat ggtgggcgaa catcatttcg cctgcttggt ccaagccggt ttctgcgttg
    43561 aaatgcggct cgatggcttg gatacgcagg tttgcgacga tgttgacggc ttcgattttg
    43621 caggtcggct cgccgacgtt gaactcgccg tccacatcga aataaatgct gacggcaggg
    43681 cggtatgcgc ctgcatcgag gctgaacgcg gtaatccagt tttcgggcag catcgtgatt
    43741 ttgccgccgg ggaaataaac cgtactcaac cgctgcataa tgatttgttc cattccgccg
    43801 ccttgtcgga cggcaagcga cggcgcggcg atgtggatgc cgacgcgctt cgtgccgttg
    43861 cccaaatcgg tcaggctcaa ggcatcgtcc acttcggtgg tcgattcgtc gtcaatggaa
    43921 aaggcggtaa cgtcggcctt gggcaggtcg ggcatttcgg gaagggagag gtcggggaaa
    43981 cctgttcctt tggggaagta tttgatttca aagccgtctt gcaggtattg cggaatggac
    44041 gtaatgccgc ccgttttttt cgccagttcg taggcggagg ttttcagtgc gtcggcggct
    44101 ttggtaaagg ctttgtaggt cagcgactgt ttgtcgggcg cgtgtaagat ggttctcaaa
    44161 tccgccgcga tttcagacgg catctcgccg cgtttcaagg cttccgccca agcgtcgatt
    44221 tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagcg cttgtttcaa agtttcttcg
    44281 ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat acaccggcgc ggcgtacagc
    44341 gcaatcaaag tcgccgccag ctcggttttg gtcggcgcat ggccgtaata ttcttcggcg
    44401 atggcttcgg cggtaaactc ctcttcgccg catacttccc acaataaatc ggtgtcgatg
    44461 tccgccgcct gcgcctgcgc gttttccaaa aacgccgcca tatcgccgtc aaactcggca
    44521 aagacattgt ttgccttcac tttggtgcgt ttgccgtgtt gggtatcgac ttggtaggta
    44581 gcgtcgtttt tttggacgac gacggcaact ttgaactggc cggactcttc gtaaaaaata
    44641 ttcatttttc ggatttttct gtggaaactc aagcgggcga ttttagcaga ttaccgaaaa
    44701 tgccgtctga aaaaaggttg ggggaaggcg ggtgcggctt tgcggtgctt gcgttgcgtt
    44761 tttgacgggg tacaatcgcc gtttttgaac ggaggacgga atggataatg taaaccgcgt
    44821 gccggagcag gcgcgttatg atgccgaacg caggcaggca gacgaagcat tggcggacgt
    44881 gtttccggca gtcagtattt tcggcagcgc gcgcacgccg cagaatcata cggattatgc
    44941 gtttgcctgc cgtctggcgc ggcggctgtc ggattcgggt attgccgtca tttcgggcgg
    45001 cgggccgggg attatggagg cggcaaacaa gggcgcgttt gccgggaagt cggtttcggt
    45061 agggctgaac attgccctgc cgcacgagca gaaaccgaat ccgtatcagg acatcgcctt
    45121 gcggttttcc cgttttgccg aacgcaaggc agtgtttttc cgctattccc aagcatatgt
    45181 cgtgatgccg ggcggcttcg ggacgctgga cgaattgttt gaaatcctga ccttggtgca
    45241 gacgggcaaa gtgccgccgc gtccggtcgt tttggttgga aaggcgtttt ggtcgggctt
    45301 ggcggagtgg ataaacgcgc agcttttggc gcgcggtatg atttccgaag gggccgcttc
    45361 tttgttctcc atatcggacg acgaagacga aatcattgcg tatctgtcgg aacacgggct
    45421 tcagacggca tagcgtcctg agagtgatgt ataattgcaa acaatttgtc aatttttgat
    45481 gtcttttccg aacaggatgc cgatatgatc aaccccatcg cctcgctttc ccccctagac
    45541 ggccgttatg cccaatccgt tgaagcattg cgcccgattt tttcagagta cggactgatg
    45601 aaggcgcgcg tcaaagtcga attaaactgg ctcaaagccc tggccgccga gccgaagatt
    45661 gccgaagtgc cgcccttcag tgccgaaacg cttgccgaaa tcgacaaagt catcgaaaac
    45721 ttttcattag aagacgcggc cgccgtcaaa gccatcgaag ccacgaccaa tcatgatgtc
    45781 aaagccatcg aatattggct gaaaaaacgt tttgccgaag tgccggaagt cgccgccgtg
    45841 agcgagttca tccacttcgc ctgcaccagc gaagacatca acaacctgtc ccacgccctg
    45901 atgttgcagc aagcgcgcga aaccgtcatc ctgcccaaac tggcagaaat catcggaaag
    45961 ctcaccgata tggcgcacga gcttgccgcc gtcccgatga tgagccgcac ccacggccag
    46021 cccgccacgc cgaccacttt gggcaaagaa accgccaatg tcgtgtaccg cctgcaacgc
    46081 cagttcaaaa tccttcaggc gcaagaattt ctcggcaaaa tcaacggcgc ggtcggcaac
    46141 tacaacgccc acatggccgc ctatcccgat gtggattggg aaacacactg ccgcaacttc
    46201 gtcgaaatca gcctcggcct gaccttcaac ccctacacca tccaaatcga gccgcacgac
    46261 tatatggcgg agtttttcca aaccctcagc cgcatcaaca cgattctcat cgacttcagc
    46321 cgcgacgttt ggggttatat ttcattgggt tatttcaaac aaaaagtcaa agcgggcgaa
    46381 gtcggctctt ccaccatgcc gcacaaagtc aaccccatcg actttgaaaa ctccgagggc
    46441 aacctcggca tggcaaacgc cgtattgggc tttttggcgg aaaaactgcc cgtctcacgc
    46501 tggcagcgcg atttgaccga cagcaccgta ttgcgcaata tgggcgtggg cgtgggctat
    46561 gccgtgttgg gtttcgccgc ccacctgcgc ggtctgaaca agctcgaacc caaccctgcc
    46621 gcgcttgccg ccgatttgga tgtcacttgg gagctgctcg ccgagccgat tcaaaccgta
    46681 atgcgccgtt acggtgtcgc caatccttac gagaagctga aagacctgac gcacggtaaa
    46741 gacggcatta cgcccgaagt gttgaaactc tttatcgaat cgttggaaat tccggcagaa
    46801 gccaaagcca aattgcttga gctgacccct gcgttgtatg tgggcaaggc tgaagcgttg
    46861 gcgaaacgga tttgagtgct tattgaaacc gatgccgtcc gaacgcgcgt tcagacggca
    46921 tttttaagat aacgggacat acggggcgat atttatgcaa gctgtccgat acaggcctga
    46981 aattgacgga ttgcgggccg tcgccgtgct atccgtcatt attttccacc tgaataaccg
    47041 ctggctgccc ggaggattcc tgggggtgga cattttcttt gtcatctcgg gattcctcat
    47101 taccggcatc attctttctg aaatacagaa cggttctttt tctttccggg atttttatac
    47161 ccgcaggatt aagcggattt atcctgcctt tattgcggcc gtgtcgctgg cttcggtgat
    47221 tgcctctcaa atcttccttt acgaagattt caaccaaatg aggaaaacca tagagctttc
    47281 tacggttttc ttgtccaata tttatttggg gttccgattg gggtatttcg atttgagtgc
    47341 cgacgagaac cccgtactgc atatctggtc tttggcggta gaggaacagt attacctcct
    47401 gtatcccctt ttgctgatat tctgttacaa aaaaaccaaa tcactacggg tgctgcgtaa
    47461 tatcagcatc atcctgtttc tgattttgac cgcatcatcg tttttgccgg ccgcgtttta
    47521 taccgacatc ctcaaccaac ccaatactta ttacctttcg acactgaggt ttcccgagct
    47581 gttggtgggt tcgctgttgg cggtttacgg gcaaacgcaa aacggcagac ggcaaacaga
    47641 aaatggaaaa cggcagttgc tttcattact ctgtttcggc gcattgcttg tctgcctgtt
    47701 cgtgatcgac aaacacgatc cgtttatccc gggaataacc ctgctccttc cctgcctgct
    47761 gacggcgctg cttatccgga gtatgcaata cgggacactt ccgacccgca tcctgtcggc
    47821 aagccccatc gtatttgtcg gcaaaatctc ttattcccta tacctgtacc attggatttt
    47881 tatttccttc gcccattaca ttacaggcga caaacagctc ggactgcctg ccgtatcggc
    47941 ggttgccgcg ttgacggccg gattttccct gttgagctat tatttgattg agcagccgct
    48001 tagaaaacgg aagatgacct tcaaaaaggc attttttctg cctttatctc gccccgtccc
    48061 tgatacttgt cggttacaac ctgtattcaa gagggatatt gaaacaggaa cacctccgcc
    48121 cgctgcccgg cacgcccgtt gctgcggaaa ataattttcc ggaaaccgtc ttgaccctcg
    48181 gcgattcgca cgccggacac ctgcgggggt ttctggatta tgtcggcggc agggaagggt
    48241 ggaaagctaa aatcctgtcc ctcgattcgg agtgtttggt ttgggtggat gagaagctgg
    48301 cagacaaccc gttgtgccga aaataccggg atgaagttga aaaagccgaa gccgttttca
    48361 ttgcccaatt ctatgatttg aggatgggcg gccagcccgt gccgagattt gaagcgcaat
    48421 ccttcctgat acccggattc caagcccgat tcagggaaac cgtcaaaagg atagctgccg
    48481 tcaaacccgt ctatgttttt gcaaacaaca catcaatcag ccgttctccc ttgagggagg
    48541 aaaaattgaa aagatttgct ataaaccaat acctccggcc tattcgggct atgggcgaca
    48601 tcggcaagag caatcaggcg gtctttgatt tgattaaaga tattcccaat gtgcattggg
    48661 tggacgcaca aaaatacctg cctgaaaaca cggtcgaaat acacggccgc tatctttacg
    48721 gcgaccaaga ccacctgacc tatttcggtt cttattatat ggggcgggaa ttccacaaac
    48781 acgaaagcct gctcaagcat tcccgaggcg gcgcattgca gtagcctgcc ttcttgccgg
    48841 atattgcctt tggcagccta tgccgctgtt tgccctttga ggcggctttt attttgccgt
    48901 tttgaggccg gggtcgggaa taaccgtttt ttgatgattt tccctccccg gctgtgtcat
    48961 caaatcccta gttgcctttc caaactctcc accagattgt catccagttt caaagcctgc
    49021 gacaggcggg cgaggaagac ggtttctttc cgcgacaaat cggcacagac caaccttgcc
    49081 gccagatagg tctccgccgc caacgcctca tcgttgccga cggcggcggc gatgtccccg
    49141 atgcttgcgg gcaggcggta ttcggcggcg agccatgcgg cagtttcggg gtctgtgccg
    49201 ctttcctgtt cgatagtccg gcgttcggct tcgtctatca tgccgtctga agcggcggcg
    49261 gctatcatgg tacgcaatac ggtgcggctg tatgtttctt cggtttttcc ggcaggttgg
    49321 aaatcgcttt gtgttacggt tgcccgccct ttgttttgct gccacatctg atagccccgg
    49381 taggcgaggt agcccaaagc ggcggtcgaa ccgattttgg tgatggtttt gcggttttta
    49441 ccgttcagca gcatggaggc gacaccggca accagcgcgc ctccgccgaa tgaattgagc
    49501 gggctgccgg agaatgtgtt gccttttttt tgaaccgtgc ttaagacttg gttgagcagc
    49561 cgggtgaagt tcatgatttt tcctttctgt tgcgggaagg gcggtatgtt taccctatcc
    49621 ttttaaacgg tggcaggccg gccaataatt gttggccgta ccgctgcgtt ttgatgcggt
    49681 tgtcgaggat ggttacgcgg ccgtagtctt gttcggtgcg gatgaggcgg ccgacggcct
    49741 ggatgagttt gatgccggct tcggggacgg tgatttcgat gaaggggttg ccgccgcgtt
    49801 gttctatcca acggttttgg gttttttcga tggggttgtc gggcatggcg aaggggagtt
    49861 tggcgatgat gacttgcacg caggcggtgc cgggcaggtc gagcccttcg gcgaagctgt
    49921 cgagtccgaa gatgatgctg gctttgcctt cttctatggc ttggtggtgt ttttgcagga
    49981 ggacggcttt gggtaattcg ccttgtacga gcaagagcgg caggtagtct ccgggcaggt
    50041 gcagggcgac atcctgcatt tgtttgcgcg aggaaaacag gacgagtgtg ccgatggctt
    50101 cggtgggcga aatcagcttg ggcagccatt cgatgacggc ggcggtgtgg gcttcggggt
    50161 ctttggggct ggcgtatatg gaggggatgt agagttcgcc ctgtttttca aagtcaaagg
    50221 ggcttttgag ggcgagggtg gtggtttcgg gcagccacag caggccggtt tggcgcaaaa
    50281 tcaagttgaa gctgccgagc gattgcaggg tggccgaagt cagcaccgcg cctgccgcgc
    50341 gccgccacag gctgttggca aggtgggacg cgctgctgat ggggctggca ttgaaaatat
    50401 agtcgttttt gtcgtcggca cggcgggcta tccatttcgc caacggctcc tcgccctcaa
    50461 tggaaacagt ggagagcaaa tcccaaaccg cgctgatttg ttcgatacgg gcgataaaaa
    50521 ggccgaactc gccggtcagg cggtcgatga gcgcaccgtc ctgttctttt tcgcggcgtg
    50581 cggcggaaag cgcatcgttc agcccgacaa cgtgtttgag caggctgcgt gcagcaatgg
    50641 ccgtattgga aacggtggtt tcgaggcctt cggggatttt gccgtcttcc cacagccaag
    50701 tcggttcgct gttggttcgt ctgtcgtttt cagacagccc catacttaaa gacggctctt
    50761 ccgccaaatg gaattgccat tcgtgcaggc tgtcgagcaa ggatgcggcg gcttcgtcgg
    50821 caaggttggc aagttcggct ttatcggtaa gcgcggcaat tttgccggta agctgcggca
    50881 gtttttccag cgtccaaacg gcaatattcc atgaatgttc ggcggcaaaa cggctgaggg
    50941 cttttttggg caggtggtgc gcttcgtcta tgcaatagaa actgttttcg ggcgcaggca
    51001 gaatcacgcc gccgcccatg ctgatgtcgg caagcagaag atcgtggttg gcaacaacga
    51061 catcgacggt ttccaacata tcgcgtgcca ggtaaaaagg gcattccgga cggttgggac
    51121 aggcggcttt caggcagccg tggcggtcgt tggtcacttt gagccaaatc gcgtcatcga
    51181 ttttttccgg ccaagcgtcg cggtcgccgt tgaaccgtcg ggcggaaaat tcgtcggcga
    51241 tgtcgcgcag cagcttcaat tcttccggct tgggtttgct gtcccacaag acggcggggg
    51301 cttcaaagcc gagcaggttt tgctgggcgt tgctttgcgt cagttgatag agtttgtagg
    51361 ggcagagata gcggccgcgc cctttggcaa gtgcgaaggt cagttccaaa ccgctttttt
    51421 cgaccagaaa cggcaggtcg cggtctacca actgctcctg caaggcaacc gtcgcgctgc
    51481 tcacaatcag ccgcttgccg cgtgtttgcg ccatgatgcc gcccgcaagc aggtaggcaa
    51541 gcgatttgcc cacgccggtc ggcccttcga ttacggcaat gctttcgccc tcgcgcttgg
    51601 gcggctcgcc gccttcttcg cgcgtcaacg tccgcgaaaa agcgttggca accgccgcaa
    51661 tcatttcccg ctgcgaagca cgcggacgga aatcgggcag gtttttgccg atattttggt
    51721 aatggtcgcg gatggcgttt ttttctaaat cggtgagcat agtatcggtg ggcggatgat
    51781 ttggggtcat tttaacattg cgcggatgcg gtacggtatc gttgtcggac ggtggttcgg
    51841 gcatatctga ttattgaaag taaaaaaacg gaagatattg ggtcatccgt acaaaatgcc
    51901 gtctgaagcg ggaaacgggc ttcagacggc attgccgcgc ttatacgccg gcgacggggc
    51961 gggcgggata gccgtgggta tcgaccgaac aggcaaaccg ccaaggcgtg tggacggtgt
    52021 cggcggacag gtgggcgagt tcgggaatgt gccgtttgat aaaagtaccg tccgggtcgg
    52081 tacggtatga ggcggcggca atgtcggggc aggtgtgccg tgaggcggca agccgccagt
    52141 tgccttggtt gattgccgca tcgaaatcgg tcagctgccg ggcaaaccat atctcgcctt
    52201 cgcggcgggg gaggtttaaa acgtggcaga aaaaatccgc gctcaagcgt ctcagggcgg
    52261 ggtggaggct gccggttttg tgcaaacagc gcatcgcggc atcgataatc ggaatgccgg
    52321 tccggccctg ctgccaaagc gtcaggcgca gggtgtgttc cggattgccg tctgaagggt
    52381 cgtcatccgc gtgctgcaag gcaagttgaa ggaaaaaatc gcggcggatg atgttgtccg
    52441 cccacgcgtt cagacggcgt tcgaggcttt cccgcgcgag caggcgcggc gagatgcagc
    52501 cgacactcaa atacgcgccc atcagcgaag tgtttttgcg cgaagggaaa tcctttaaaa
    52561 cggagtagga agccgcctgt tcgagaaacc gccgccactg ccgccaagcc gccgtttcgc
    52621 cgccgttttg cggcaggaag atgccgtctg aaagcgcggc aggcggcggg gcgggcaggt
    52681 tttcggggaa gggttggcgg tatgccgcga ataggtccga accggcgggg ggctgcttgg
    52741 aaaagcggtc gagccatact tcgcggtagc ggttgaaatc ggtgtgtgcc gtaccgttgt
    52801 ctggtgtcag gtcggttttg ccgaaaacgg aacggtcgtt gacgaaggtt aacgcgatgc
    52861 cgtgtttgtc caattcgtgc caaagggcgt tgtcggcgag tttgtcggca aaagtatggg
    52921 attcgtcggc gatgacggcg cggatattga ggcggacggc gagccggaca agcccggcag
    52981 gcgatgccgc cgtgtagagc gggatgccgc gccttgccag cccttgggcg agttcggcgg
    53041 cggattggcg gtagaacgcg gcgcggcgcg ggttgtctgt ttcggcatcg tcaatccaaa
    53101 tgccgataac gggcgaacct tcggcaacgg cggcgcataa ggcggcgttg tcgcggatgc
    53161 ggaggttttg gcggaaccag acgagcgtgt gcgcggcgcg cgtgtccgca taaaggggga
    53221 gggcggtttc agacggcatt tcggcagcct ttcctgctgg cgattttttc gttcagaaaa
    53281 tcgatgaaac tgcggacttt cgcactcaaa aatgccctgt ccgcataaac ggcgttcagc
    53341 cggtcggtcg ggacggcgta tccgggcagc agcctcacca gcgggccgca gcgcaaatcg
    53401 tgttccgccg cccaaagcgg ctgataaccg atgcacgcgc ccgccttaat catttcgcgc
    53461 atcatcagcg tgttgtcggt acggatgacg ggggtcagtt caagccggta tttcttgccg
    53521 tccgatttgc gggtgaggtc gagtttctgc tggttggtgt aggtcggcag gacggcaggc
    53581 agccccgcca cttcttccgg tgtttccggc acgccgttgc gcctcaggaa gtcgggcgag
    53641 gcaagcaggg caaattcgat ttccgccagc gggcgcgcaa tcagcgaagg ggaaagggtt
    53701 tgggaaacgc gcaacgccaa atccacgcct tcggcaatca aatcgacgtg gcggttgtcc
    53761 aaaatcagtt ccaatgccac ttcgggataa cgttcgcggt attccgccag ccagttgcat
    53821 atctggctgc cggcaaacca cagcggcatc gttacgcgca gcagcccctg cggtttttcc
    53881 gtccccccgg cggctttttg cgcggcatcg tcgagcgtgt cgagcgcgta actgcattgc
    53941 cggtagtatt cttccccggc ttcggtcagg ctgaggttgc ggctgttgtg gtgcaggagt
    54001 ttggcttgga cggtgttttc caagtgactg acgtgtttgc ttgccattgc ggtggagatg
    54061 ccgagcgcgt cggcggcgcg ggtgaagccg ccgctttgga cgacttggcg gaaaactttg
    54121 aggctgaaca gggtgtccat attttcttgt gtggaaaagt tgtatcaata aaagcagtat
    54181 atatttgaaa aagggaaaca tctatactct gccgcctgaa atgaagacaa atatcaaagg
    54241 agcttttatg tccgattgct gcaaccgtat ccaacctgtt ttgctttctg ttttgcgtat
    54301 cgtaaccgcc tacctgtttt tgttgcacgg tacgtcgaaa atcttcgcct tccccattga
    54361 aatgggcagc ggttcgtccg gcgggctgtt gctgcttgcc ggtattttag aaattgtcgg
    54421 cggcattttg ctggtgttgg gcctgtttgc gcgccctgcc gcgtttgttt tgtccggcca
    54481 gatggcggtt gcctatttta tggcgcacgc ttccggaaat gctttgttcc cgattgccaa
    54541 cggcggcgag tccgcagtgc tgttctgctt cgtatttctc tatatcgcgg cagcgggcgg
    54601 cggagcatgg tcgctggaca ggctgttttt caagcgtaaa gcctgaaccg gactgcctaa
    54661 agtgtatttt gttgaatgtt tttgaggaaa agaaatgagc cgtcaatccc tgcaacaggc
    54721 tgccgaaagc cgccgttccg tttattcgtt aaataaaaac ctgcccgttg gcaaagatga
    54781 aatcgtccaa atcgtcgaac acgccgtttt gcacacacct ttttcgttca attcccaatc
    54841 tgcccgcgtg gtcgtgctgt ttggcgaaga gc
//
LOCUS       DAXFHF010000004        53152 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXFHF010000004 DAXFHF010000000
VERSION     DAXFHF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 53152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53152
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.004"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            202..1284
                     /locus_tag="ACFIRM_000174"
     CDS             202..1284
                     /locus_tag="ACFIRM_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC3027912.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNSPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            1281..3074
                     /locus_tag="ACFIRM_000175"
                     /pseudo
     CDS             1281..3074
                     /locus_tag="ACFIRM_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            3101..4866
                     /gene="cysI"
                     /locus_tag="ACFIRM_000176"
                     /pseudo
     CDS             3101..4866
                     /gene="cysI"
                     /locus_tag="ACFIRM_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            5039..6898
                     /gene="ilvD"
                     /locus_tag="ACFIRM_000177"
     CDS             5039..6898
                     /gene="ilvD"
                     /locus_tag="ACFIRM_000177"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC3027913.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            6940..7242
                     /locus_tag="ACFIRM_000178"
     CDS             6940..7242
                     /locus_tag="ACFIRM_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027914.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            7280..8053
                     /locus_tag="ACFIRM_000179"
     CDS             7280..8053
                     /locus_tag="ACFIRM_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC3027915.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGNITRR"
     gene            complement(8149..9201)
                     /gene="bioB"
                     /locus_tag="ACFIRM_000180"
     CDS             complement(8149..9201)
                     /gene="bioB"
                     /locus_tag="ACFIRM_000180"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC3027916.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(9408..10784)
                     /gene="mpl"
                     /locus_tag="ACFIRM_000181"
     CDS             complement(9408..10784)
                     /gene="mpl"
                     /locus_tag="ACFIRM_000181"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC3027917.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(10846..11151)
                     /locus_tag="ACFIRM_000182"
     CDS             complement(10846..11151)
                     /locus_tag="ACFIRM_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3027918.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKDCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            complement(11163..11516)
                     /locus_tag="ACFIRM_000183"
     CDS             complement(11163..11516)
                     /locus_tag="ACFIRM_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027919.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEEQ"
     gene            complement(11778..11975)
                     /locus_tag="ACFIRM_000184"
     CDS             complement(11778..11975)
                     /locus_tag="ACFIRM_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027920.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNAVAAAVLFWIWWKWF"
     gene            complement(11985..12770)
                     /locus_tag="ACFIRM_000185"
     CDS             complement(11985..12770)
                     /locus_tag="ACFIRM_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC3027921.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            complement(12844..14154)
                     /gene="tilS"
                     /locus_tag="ACFIRM_000186"
     CDS             complement(12844..14154)
                     /gene="tilS"
                     /locus_tag="ACFIRM_000186"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC3027922.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            complement(14222..15181)
                     /locus_tag="ACFIRM_000187"
     CDS             complement(14222..15181)
                     /locus_tag="ACFIRM_000187"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC3027923.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15393..15794
                     /locus_tag="ACFIRM_000188"
     CDS             15393..15794
                     /locus_tag="ACFIRM_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3027924.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            complement(15862..16059)
                     /gene="iscX"
                     /locus_tag="ACFIRM_000189"
     CDS             complement(15862..16059)
                     /gene="iscX"
                     /locus_tag="ACFIRM_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC3027925.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            16272..16812
                     /locus_tag="ACFIRM_000190"
                     /pseudo
     CDS             16272..16812
                     /locus_tag="ACFIRM_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(16872..17213)
                     /gene="fdx"
                     /locus_tag="ACFIRM_000191"
     CDS             complement(16872..17213)
                     /gene="fdx"
                     /locus_tag="ACFIRM_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC3027926.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(17269..18030)
                     /locus_tag="ACFIRM_000192"
     CDS             complement(17269..18030)
                     /locus_tag="ACFIRM_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC3027927.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERAEHDAAWGIDFGDPGQILPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            complement(18066..18714)
                     /locus_tag="ACFIRM_000193"
                     /pseudo
     CDS             complement(18066..18714)
                     /locus_tag="ACFIRM_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(18900..20762)
                     /gene="hscA"
                     /locus_tag="ACFIRM_000194"
     CDS             complement(18900..20762)
                     /gene="hscA"
                     /locus_tag="ACFIRM_000194"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC3027928.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            20933..21805
                     /gene="hpnD"
                     /locus_tag="ACFIRM_000195"
     CDS             20933..21805
                     /gene="hpnD"
                     /locus_tag="ACFIRM_000195"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC3027929.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            21880..23193
                     /gene="hpnE"
                     /locus_tag="ACFIRM_000196"
     CDS             21880..23193
                     /gene="hpnE"
                     /locus_tag="ACFIRM_000196"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC3027930.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            23275..23994
                     /locus_tag="ACFIRM_000197"
     CDS             23275..23994
                     /locus_tag="ACFIRM_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3027931.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKKFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            24160..24831
                     /locus_tag="ACFIRM_000198"
     CDS             24160..24831
                     /locus_tag="ACFIRM_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC3027932.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            24841..25207
                     /locus_tag="ACFIRM_000199"
                     /pseudo
     CDS             24841..25207
                     /locus_tag="ACFIRM_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            25204..25851
                     /locus_tag="ACFIRM_000200"
     CDS             25204..25851
                     /locus_tag="ACFIRM_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC3027933.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            26244..28010
                     /locus_tag="ACFIRM_000201"
                     /pseudo
     CDS             26244..28010
                     /locus_tag="ACFIRM_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            28114..28782
                     /gene="hda"
                     /locus_tag="ACFIRM_000202"
     CDS             28114..28782
                     /gene="hda"
                     /locus_tag="ACFIRM_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC3027934.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            28779..29447
                     /locus_tag="ACFIRM_000203"
     CDS             28779..29447
                     /locus_tag="ACFIRM_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3027935.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEFITPHISPMQHMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(30000..30896)
                     /gene="argB"
                     /locus_tag="ACFIRM_000204"
     CDS             complement(30000..30896)
                     /gene="argB"
                     /locus_tag="ACFIRM_000204"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC3027936.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARNVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            31066..32196
                     /locus_tag="ACFIRM_000205"
     CDS             31066..32196
                     /locus_tag="ACFIRM_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC3027937.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            32270..33121
                     /gene="lgt"
                     /locus_tag="ACFIRM_000206"
     CDS             32270..33121
                     /gene="lgt"
                     /locus_tag="ACFIRM_000206"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC3027938.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(33206..33868)
                     /locus_tag="ACFIRM_000207"
     CDS             complement(33206..33868)
                     /locus_tag="ACFIRM_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC3027939.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            33902..34008
                     /locus_tag="ACFIRM_000208"
                     /pseudo
     CDS             33902..34008
                     /locus_tag="ACFIRM_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34067..35860)
                     /locus_tag="ACFIRM_000209"
     CDS             complement(34067..35860)
                     /locus_tag="ACFIRM_000209"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC3027940.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            36106..37215
                     /gene="proB"
                     /locus_tag="ACFIRM_000210"
     CDS             36106..37215
                     /gene="proB"
                     /locus_tag="ACFIRM_000210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC3027941.1"
                     /translation="MKYKRIVFKVGTSSITRSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIKMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            37228..38490
                     /locus_tag="ACFIRM_000211"
     CDS             37228..38490
                     /locus_tag="ACFIRM_000211"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC3027942.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADAEEKNRALLAMADSLEAAAEDI
                     LAANRLDLKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLER
                     LLVRGRIEAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEARGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(38666..41710)
                     /locus_tag="ACFIRM_000212"
     CDS             complement(38666..41710)
                     /locus_tag="ACFIRM_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3027943.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKTAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            41875..42555
                     /locus_tag="ACFIRM_000213"
     CDS             41875..42555
                     /locus_tag="ACFIRM_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027944.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            42584..42943
                     /locus_tag="ACFIRM_000214"
     CDS             42584..42943
                     /locus_tag="ACFIRM_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC3027945.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            43021..43127
                     /locus_tag="ACFIRM_000215"
                     /pseudo
     CDS             43021..43127
                     /locus_tag="ACFIRM_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(43548..44084)
                     /locus_tag="ACFIRM_000216"
     CDS             complement(43548..44084)
                     /locus_tag="ACFIRM_000216"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC3027946.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(44118..44474)
                     /gene="folB"
                     /locus_tag="ACFIRM_000217"
     CDS             complement(44118..44474)
                     /gene="folB"
                     /locus_tag="ACFIRM_000217"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC3027947.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            44530..45132
                     /gene="plsY"
                     /locus_tag="ACFIRM_000218"
     CDS             44530..45132
                     /gene="plsY"
                     /locus_tag="ACFIRM_000218"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC3027948.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            45224..46069
                     /locus_tag="ACFIRM_000219"
     CDS             45224..46069
                     /locus_tag="ACFIRM_000219"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC3027949.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(46178..46510)
                     /locus_tag="ACFIRM_000220"
     CDS             complement(46178..46510)
                     /locus_tag="ACFIRM_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC3027950.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(46560..46808)
                     /locus_tag="ACFIRM_000221"
     CDS             complement(46560..46808)
                     /locus_tag="ACFIRM_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027951.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(46865..47026)
                     /locus_tag="ACFIRM_000222"
     CDS             complement(46865..47026)
                     /locus_tag="ACFIRM_000222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027952.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVGKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            47051..48025
                     /locus_tag="ACFIRM_000223"
     CDS             47051..48025
                     /locus_tag="ACFIRM_000223"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC3027953.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     ADVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(48184..48402)
                     /locus_tag="ACFIRM_000224"
     CDS             complement(48184..48402)
                     /locus_tag="ACFIRM_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC3027954.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            48595..48858
                     /locus_tag="ACFIRM_000225"
     CDS             48595..48858
                     /locus_tag="ACFIRM_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3027955.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            48981..50766
                     /gene="ggt"
                     /locus_tag="ACFIRM_000226"
                     /pseudo
     CDS             48981..50766
                     /gene="ggt"
                     /locus_tag="ACFIRM_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574051.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            50830..50994
                     /locus_tag="ACFIRM_000227"
     CDS             50830..50994
                     /locus_tag="ACFIRM_000227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027956.1"
                     /translation="MSNQTKIQAIKQTSEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            50939..51181
                     /locus_tag="ACFIRM_000228"
     CDS             50939..51181
                     /locus_tag="ACFIRM_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027957.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            51248..52498
                     /gene="glyA"
                     /locus_tag="ACFIRM_000229"
     CDS             51248..52498
                     /gene="glyA"
                     /locus_tag="ACFIRM_000229"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC3027958.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(52571..52960)
                     /locus_tag="ACFIRM_000230"
     CDS             complement(52571..52960)
                     /locus_tag="ACFIRM_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3027959.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
BASE COUNT        12539 a        15136 c        13359 g        12118 t
ORIGIN      
        1 gccggggcgg gacaacgccg taccggtttt tgttcatccg ctataaaacg gaaaaccgcc
       61 gcatccgcag ccgcccccca tatagctttc agcttctaac aaatgccttt ttattattta
      121 aaatgacccg cattccgaca aaaatgccgc gacgcaccgt atcggaaaca aggtgcgggc
      181 aaacagcaaa ggagtcagcc agtgaattat tttcccattt tcgccaacct cgccggccgc
      241 cccgtattgg tcgtaggcgg cggcgcggtc gccgcacgca aaatcagcct gctgctgaag
      301 gcgggcgcag aggtcggggt tgccgcaaaa cacctgaatg ccgaactctc cgcgttggcg
      361 gcggaaaaca aaatcctgtg gcttgccaaa gaatttcgtg ccgaacacat ccgcacggtt
      421 ttcctcatca ttgcggcaag cagcgaccaa gccctcaacc ggcgcgtttt ccatcttgcc
      481 gaaagctgcc aaaagccggt caacgtggtg ggcgaccgtg accattgcag cttcatcttc
      541 ccgtcggtta tcgaccgcga ccctgttcag attgccgttt ccagctccgg cagcgcgccc
      601 gtcctcgccc gcctgctgcg tgaaaggctg gaagccttgc tgccgccgtc tttgggcgat
      661 atggcggaaa tttcaggaag gtggcgcgat gccggttttt atttccccgt ccaactggtc
      721 gtccgccaag atgccgacaa ggccgacggt ttccgaggct atcaggggcg catcgaacgc
      781 ggttcggtca ccgtcgggca aaccgtccgc atcgaaccga acgggctgac cgccgaagtg
      841 tccgaaatca tcgcgccgaa aggggaagtg gcgcaggctt ttgccggcga ggcggcaacc
      901 atccggctgg accgcgacat cgatgtttcg cgcggcgatc tttttgtcga taaaaattcc
      961 ccgctcgccc cgcaaaaaca tccggaagcc acgctttgct ggtttgacga acgcccgctc
     1021 aacaccgcgc gcaaatacct gctcaagcac ggcacgcaaa ccgtgccggc aaaagtcggg
     1081 gaaattgaaa gcgttttgga tgtccgcacg ctggaacaag aggccggcgc ggaatcctta
     1141 aagatgaacg acatcgccaa agtccgcatc aaccttcaaa aacccgttac ggcaacgcct
     1201 tatgcggaaa acaccgccgc cggttcgttt atcctgatag acgaagcgac atacggtact
     1261 gttgcggcag gtatgatttt atgagtgaac acgatatgca gaacacaaat ccgccattac
     1321 cgcctatgcc gcccgaaatc acgcagctcc tgtgtcgggg ctgtccggct acgcttgggc
     1381 aaaagcagga aacggggcat ccgcagggct gcccgcgctt cagacggcat tgccggcggc
     1441 agaacctttt tccgtaaccg tcctttccgc ctcgcaaacc ggcaatgcga aatccgttgc
     1501 cgacaaagcg gcggacagcc tgaaagccgc cggcatccaa gtcaggcgcg ccgaactgaa
     1561 aggctacaag gcgaaaaaca tcgccggcga acgccgcctg ctgctggtta cgtccaccca
     1621 gggcgaaggc gaaccgccgg aagaagccgt cgtgctgcac aaactgctga acggcaaaaa
     1681 agccccgaaa ttggacaaac tccaatttgc cgtactgggt ttgggcgaca gctcctatcc
     1741 gaatttctgc cgggcgggca aagatttcga caaacgtttt gaagaattgg gcgcaaaacg
     1801 cctgctcgaa cgcgttgatg cggatttgga ctttgccgcc gccgcagacg gatggacagg
     1861 ccgcattgtc gcgcgcttaa aagaagaagc cgcaaaaaac cgggcaacgc ccgcgccgca
     1921 ggcaacgtcc ccccgccggc cttcagacgg catcggaggg caggtactgc aaggcagacc
     1981 cctttcccgc cgccctgctg gccaatcaga aaatcaccgc ccgccaatcc gataaagacg
     2041 tgcgccacat cgaaatcgat ttgagcggtt cggatttgca ctacctcccg ggcgacgcgc
     2101 tcggcgtttg gtttgacaac gatccggcac tggtcgggga aatcctagac ctgctcggca
     2161 tcaatccggc aacggaaata caggcgggcg gaaaaaccct gccggttgcc tccgcactgt
     2221 tatcccattt cgaactcacg caaaacaccc ccgcctttgt caaaggctat gccacgttcg
     2281 ccgatgatga cgaactcgac cgtattgctg ccgacaacgc cgttttgcaa ggctttgtgc
     2341 aaagcacgcc gattgccgat gtgctgcacc gcttcccggc aaaactgacg gcggaacaat
     2401 tcgccggcct gctgcgcccg cttgcgccgc gcctatattc gatttcctcg tcgcaggcgg
     2461 aagcggggga cgaagtgcac ctgaccgtcg gcgcagtgcg tttcgaacac gaagggcgcg
     2521 ccagggcggg cggcgcatcg ggtttctttg ccgaccggct ggaagaggac ggcacggtgc
     2581 gcgtgtttgt ggaacgcaac gacggcttca ggctgcccga agacagccgc aagccgattg
     2641 tgatgatcgg ctccggtacc ggcgtcgcac cgttccgcgc cttcgtccaa caacgtgccg
     2701 cagaaaatgc ggaaggcaga aactggctga ttttcggcaa tccgcatttt gccgccgact
     2761 tcctctatca gaccgaatgg cagcagtttg ccaaagacgg cttcctgcac agatatgact
     2821 tcgcctggtc gcgcgatcag gaagaaaaaa tctatgtgca ggacaaaatc cgcgaacagg
     2881 cggaaggact ttggcaatgg ctgcaggaag gcgcgcatat ctatgtgtgc ggcgatgcgg
     2941 caaaaatggc aaaagaagtg gaagccgcct tgctggatgt gattatcggg gcagggcatt
     3001 cggacgaaga cggcgcagaa ggatatttgg atatgctgcg cgaagaaaaa cgctatcagc
     3061 gtgatgttta ttgattaaat ataatcggga ggaacacaaa atgaccgtac aggccaagac
     3121 aaaaggtttg gcgtggcaag aaaagccgct atccgacaac gaacgtctga aaaccgaaag
     3181 caatttttta cgcggcacga ttttggacga tttgaaagac ccgctcacgg gcggcttcaa
     3241 aggcgacaac ttccaactca tccgcttcca cagtatgtat gagcaggatg gccgcgacat
     3301 ccgcaccgag gcaaaactcg agcccttgaa atttatgctt ttgcgctgcc ggctgccggc
     3361 gggatcatca aaccgtccca gtggatagaa ctggacaaat tcgcccggga aaagagtcat
     3421 taccgctcca tccggctaac caaccggcaa accttccaat ttcacggcgt gccgaaaacc
     3481 aagttgcaga cgatgccccg cctcttgcac agcctgggtt tggattccat cgctacggcg
     3541 gcggatatga accgcaatgt gctttgcacc tccaacccga tcgagtccga gctgcaccgg
     3601 caggcttacg aatacgcgaa aaagatttcc gaacacctgc tgccgcgcac gcgcggttat
     3661 ctggatatgt gggtggacgg caaaaaagtt caaagttccg acgacttcct tcaagaaggc
     3721 gagccgattt tgggcaaaac ctatctgccg cgaaaattca aaaccgcagt cgtcatcccg
     3781 cccttgaacg atgtggactg ctacggtaac gatttggatt tcgtcgccgt ttcagacggc
     3841 aacggacagc ttgccggctt caatgttttg gcgggcggcg ggctttcgac ggaacacggc
     3901 aacggcaaca ccaaaaccta tccgaacatt tcactggaac tgggtttcgt gcctccggaa
     3961 cacgcactga aggccgccga agcggtggta accacgcagc gcaacttcgg caaccgcagc
     4021 caccgcaaaa acgaccgcac ccgctacacc attcaaaata tgggcttgga caacttccgc
     4081 acggaagtcg aacgccgtat gggtatgccg ttcaaaccca tacggccgtt caagtttacc
     4141 gggcgcggcg accgcatcgg ccgggtgaaa ggcatagacg gcaactggca tttgaccctt
     4201 ttcatcgaaa gcgggcgttt ggttgacgaa ggcgggaaac agcttctgac cggcgtgttg
     4261 gaaatcgcca aaatccacaa aggcggcttc cgcatcaccg ccaaccaaaa cctcatcgtg
     4321 gcaaatgtgc ccgaaggagg caaagcccgg atcgaacagc tcgcccgctc atacgggctg
     4381 atacgcgacg gtgtcagcaa gctgcgcgaa aatgcgatgt cgtgcgcttc cttcccgacc
     4441 tgcccgctgg caatggcgga agccgaacgc gtgttgccgg acttcatcgg cgagctggat
     4501 aagattatgg cggaacacgg cgcgtcggac gactacatcg ttacccgcat taccggctgc
     4561 ccgaacggct gcggacgggc gatgttggcg gaaatcggac tggtcggcaa agccgtggga
     4621 cgctacaacc tccacatcgg cggcgaccgc gaaggcgtac gcatcccccg cctctacaaa
     4681 gaaaacatca ccctgccgga aatcctttcc gaattggaag acctgatcgg caaatgggcg
     4741 gcgggacgcg ataccgacga aggtttcggc gatttcgcca tacggacggg catcgtcaaa
     4801 cccgtgttgg atgcgcccgt cgatttttgg gacgcatcca aagccgtccc gattgcgcgc
     4861 gcctgaaccc gtcccccgca ttcctgcggg gatgttcttt tttgccgcac acgtttccat
     4921 tccgcaaaat tccgatactt ggtataaaat accccctttt cccactctaa aaaaccgtac
     4981 ccgatgccgt ctgaaacagc cctgcctttc agacggtata ggcacaagga aacacactat
     5041 gccagaatac cgctccaaaa cctccaccca cggccgcaat atggcgggcg cgcgcgcatt
     5101 gtggcgcgcc accggcgtga tggaaaccga cttcggcaag cccatcatcg ccgttgccaa
     5161 ctccttcacc caattcgtgc ccggccatgt ccacctgcac aatatgggcc agctggttgc
     5221 ccgcgagatt gaaaaagcgg gtgcaattgc caaagaattc aacaccatcg ccatcgacga
     5281 cggcatcgcc atgggacaca gcggtatgtt gtactccctg cccagccgcg atttgattgc
     5341 cgactccatc gaatatatgg tcaacgccca ctgcgccgac gcgctggtgt gcatttccaa
     5401 ctgcgacaaa atcaccccgg gaatgctgat tgccgccatg cgcctgaaca tccccaccat
     5461 cttcgtctcc ggcggcccga tggaagcggg caaggtcatc ggcgtggcaa acatccaacc
     5521 cgaacgccgt ttggacttga ttgacgccat gatcgaatcg gcggacgaca atgtcagcaa
     5581 ccggcaagtc gaagaagtcg aacaaaacgc ctgcccgacc tgcggctcgt gttcgggtat
     5641 gtttacggca aactcgatga actgcctgac cgaagcgctc ggtctgtccc tgcccggcaa
     5701 cggttcgtat ttggcgaccc acgcaggccg caaagaattg ttcctcgaag cagggcgtat
     5761 gattgtcgaa atcaccaaac gctattacga gcaagacgac gaaaccgtgc tgccgcgcag
     5821 cattgccacc aaaaaagcgt ttgaaaacgc catgacgatg gatattgcga tgggaggcag
     5881 caccaatacc atcctgcact tgctcgccgt cgccaacgaa gcgggcgtgg atttcaaaat
     5941 ggcagacatc gaccgcttaa gccgcgtcgt gccctgcatc tgcaaaaccg cgcccaacaa
     6001 ccacgactac tatatggaag acgtgcaccg cgccggcggc atcttcgcca ttctgaaaga
     6061 acttgacaaa gcgggcaaac tgcacaccga cgtgtacacg atccacgcgc cgacactgaa
     6121 agacgcgatt gaaaaatggg acgtcaccaa tcccgaaaac acccgcgcca tcgaacgttt
     6181 caaagcggct cccggcggcg tgcgcaccac tcaggcgttc tcgcaaaacc gtatttggaa
     6241 aaccctcgac cttgaccgcg aaaaaggctg tatccgcgac gtggcacacg cctactcgca
     6301 agacggcggt ttggcggtct tattcggcaa catcgccgag cgcggctgcg tggtaaaaac
     6361 cgcaggcgtg gacgagagca tcctcaaatt caccggccgc gcgcgcgtgt ttgaaagcca
     6421 ggaagccgcc gtcgaaggta ttttgggcaa ccaaatcgtc gccggcaaca tcgtcatcat
     6481 ccgctacgaa ggcccgaaag gcggcccggg tatgcaggaa atgctatacc cgacctccta
     6541 cctgaaatcc aaaggcttgg gcaaagcctg cgccctctta accgacggac gcttctccgg
     6601 cggcacatca ggtttgtcca tcggacacgc ctcgcccgaa gcggcggaag gcggcgcaat
     6661 cggtttggta cacgaaggcg atacgattga aatcgacatc cccaaacgca gcatccgcct
     6721 tgtcatttcc gatgaagagc ttgccgcacg ccgtgccgaa atggaagctc gcggcagcaa
     6781 agcgtggaaa ccggaaaacc gcgaccgcta cgtctccgcc gcattaaggg cttacggcgc
     6841 gatggcgact tccgccgaca aaggcgcggt gcgcgacgta tcgcaaatcg agagataagc
     6901 cccaaaacgt gcggcaaatg ccgtctgaac agtaaaggta tgattattaa caaatttatc
     6961 tgggcgatgg cggtattttc cgcaattttg gccctcgtta tcggcagcgg ctttaccgcg
     7021 ctcgagtatg taaaagagcc gcctgccgcc ccttatgctg tttccgcgcc gaaaaccgcc
     7081 ggggtcaagc ctcggcgcat cctgccggaa cggaatcccc cgtgctgccg attgacggtt
     7141 caaaccccgc gcccgtttca aacaccggcg atgcggacgg acaggatacg cctgacggaa
     7201 aggcagcaga taccgtttcc atcatcgggg tcggcggcat aaggtacgct gtttgacgaa
     7261 ggcggtacgc cgaaaaaatg tgcaaaccac caaaatatgc tatgcattcc gaacgccctc
     7321 cgcatacggg caataccttg ccgacgcggg attcgactac ctcagcctcg caaacaacca
     7381 cagcaacggc ttcggcgcgc aaggcatcac ggcaacggca ggcaaccttg acgaattgaa
     7441 catcaaatat tcgggcatcg aaaacaggtt tgaaaccgcc atcctgaaga aaaacggcgt
     7501 gagatacggc ttcgtctcct tcgcccccaa ccttgccgcc gtcaaactga acgattacgc
     7561 caaattcaaa aaactgattc ggaaaaccaa acaaaaaacc gacatcgtca ttgtgatgtt
     7621 ccacggcggc gcggaaggga aacaggctga acacctgccg ttcgataccg aaatcttcta
     7681 tggggaaaac aggggcaacg tcgtcgagtt tgcgcggctc gccgtcgatt ccggcgcgga
     7741 tgtcgtattc gggcaggggg cgcacgttac tcgcgccgtc gaactttacc acgaccgctt
     7801 catctcttac agcggcggca actttgccac ctacggcatc agcggcattg cgccgatttt
     7861 caaaattatc accgacaaac aggggcggtt cgtttccggc aacattatcc ccatcactca
     7921 agtcggcgat aaaatcccca aaatcgaccc tgaaaaaact gttattgagc ggattattta
     7981 tctgaaccat agcgacttcc ccaacgggaa cggactggat gtctcgcccg gcggcaacat
     8041 cacgcgccgg taaaaagata ttggaaattc cgtccgaaca aatgccgtct gaaagccttt
     8101 tttcaggttt cagacggcat tcaagtttca gacggcattt ttcaatcgtc aataatccgc
     8161 tttaatcccg gaggcttttt ccacttcggc gacctcgcct tccggctcaa actgcaaggg
     8221 atacaggtta agcttttcca tcaggattct gtcgccgtcc tcatcaggat tgcctgtggt
     8281 caacagcttg tcgccgtaaa aaatcgagtt cgcacccgcc atgaagcaca tcgcctgcat
     8341 cgcttcaggc atattgctgc gccctgccga gaggcggaca taactttgcg gcatcgtaat
     8401 ccgtgctacg gaaaccgtac ggacaaattc ggtccaatcc aaatcttcgg catcggcaag
     8461 cggcgtgcct tccactttga ccaaccggtt aatcggcacg ctttcgggct gcgggtcgag
     8521 gttggcgagg ctggcaatca gcccggcgcg ttcggcgcgg gtttcgttca tcccgacgat
     8581 gccgccgcag cagactttca aaccggcgtt gcggactttg cccaaggtat ccatacggtc
     8641 ttcgtgccgg cgtgtgtgga tgatgtcgtt gtagcggtcg gggtcggtgt cgaggttgtg
     8701 gttgtaataa tccaagcccg cctctttcaa gtcttccgcc ataccttctt cgagcatacc
     8761 gaacgtgccg caggtttcca tacctaagcc cttgacggct ttgatgattg cggaaaccgt
     8821 ctccacgtct ttgggtttgg ggccgcgcca tgccgcgccc atacaaaacc ggcttgcgcc
     8881 gcgcgatttg gcgattttgg ctttttcgac gatttcatcc acatccatca tctgctcttt
     8941 gcccaaattg gtgttgtggt gcgctgattg cggacagtag gcgcaatctt cgggacaacc
     9001 gccggttttg atggacaaca gcgtggaaag ctggatttcg cgcgggttga aattttggcg
     9061 gtggacttct gccgcctgat agacaagttc taaaaacggc agtccgaaaa gtgcctcgac
     9121 atcgcatttt ttccaatagc gcgcggtggg atgaggcttg cactcggtct tacggcgcaa
     9181 ggcgacgggg gatacggtca taatgtgttc tttcgtattt acagcggcgc aatgacgctg
     9241 acaggcaaac cgccggcgca atgacggcgg ttgcgggtaa tcaaatggct gaaaacagcg
     9301 gcggcaagtg taacgcgttt gaaaatacgg ggcaaacggt ttttccgcct ttgtgccgcg
     9361 cagccgggca atgccgtcta aagggctttc agacggcatt gcccgggcta tctcaaagcg
     9421 tccagcagtt tggtgtgtat tccgccgaaa ccgccgttgc tcatcaccaa aatatggtcg
     9481 ccggttcggg cgtttttcac aatttcggca acgaaggtat cgaaatcttt accgacgcgc
     9541 agcctgcagc ccaaaggcgc gagggcttcg gcaacgtccc agtccgcgcc gccggcgtag
     9601 cagaacactt ggtcggcttc tttgaggctt gcgggcaggg cggacttcat cgtgccgagt
     9661 ttcatggtgt tggaacgcgg ctcgaggacg gcgaggatgc gcgcgccgcc gacacgttgg
     9721 cgcaaacctt gaatcgtggt ttcgatggcg gtcgggtggt gggcgaaatc gtcgtaaacg
     9781 gtgatgccgt ttgccgtgcc tttgatttcc atgcggcgtt tgacgttttt aaacgcaccc
     9841 aaggcttcgc aggccgtctg aacatcgact ccggcatggc gtgcggcagc gatgacggcg
     9901 agcgcgttca tgcggttgtg tccgcccatc aaatcccatg cgacgtgtcc ggcttttttg
     9961 ccgtcaagca atacgtcgaa cgagccgtcg gcattgactt caccaatctg ccagccgtgt
    10021 ccggtgccga atttttccac cggcgtccag cagcctttgt ccaaagtatc ttgcaggctt
    10081 tgctgctgtc cgttgcagac gatgaggcct tcggatggta cggtgcgcac gaggtggtgg
    10141 aactgggtct gtatcgcgcc caagtcggcg aagatgtcgg cgtggtcgaa ttccagattg
    10201 ttcaacacgg cggtacgcgg gcgataatgc acgaatttgg agcgtttgtc gaaaaaggcg
    10261 gtgtcgtatt cgtcggcttc gatgacgaaa aacggcgatt tgctgttcgg gtcttgacgc
    10321 ggcgtttgcg gtaggcgggc ggaaacgccg aaattttccg gtacaccgcc gatgaggaag
    10381 cccggcgcga gtccggcata ttccaagacc caggcgagca tggacgcggt ggtcgttttg
    10441 ccgtgcgtcc ctgccacgcc gagtacccaa tgatggtgca gcacgttttc agccagccat
    10501 tgcgggccgg aaatataagg cagcccacgg ttcaaaatcg cctcgaccac atccatcccg
    10561 cgcctggcga cattgccgat gacgtaaata tcggcttgaa attcctccaa ctgcgcggca
    10621 tcgaagcctt cgtgtacgcc tatgcccaag gcttcgagct gggtgctcat cggcggatac
    10681 atcttcgcgt cgcaaccgct gactttgaac ccggcttctt tggcaatggc ggcaatcccg
    10741 cccataaacg tgccgccgat accgataatg tggatgtgtt tcatcagagt tccctaatcg
    10801 ttcaaattct attgttcaac accgtaatgg ttgattcggt ttcaatcaat gcggtctgat
    10861 gccggcatcc gaaccgtctt tctgtgcatt ccctttcgga ttttggacgg cttcgtccct
    10921 caccgtgtcc gaataatcgc caactcctgc gtcttccgcc ctgtctttca cggcaggcct
    10981 gaccgaactg tacacccgat cccgccccaa acccgcgttg gtacaatctt ttcccggagt
    11041 ttggctgtaa ctggttttac cattatggcg gcactcgtaa acatttgccg aaaatacggt
    11101 ctgaaccgct gcaacaagca atatacttcc aatcatttta gatacgcgca tattaaactc
    11161 ctttattgtt cctcttcaaa aaaaggaatg tatttcaata gaacttcatc taattctttc
    11221 attccttgct cataattgaa cgggtattcc caaccatccc ctactttgat ttcagaaatg
    11281 tctttaaact tccgcttaag ggcggacact ttgtccatat cgtcatcaat ttcctgtttg
    11341 ccgaacggta ttttccgctt ttccaaaacc tcatcataat aacaaggtaa attcagcaat
    11401 agcccccaag cgctatttgc gattacgact aatctatatt tttccctagt taagtaatct
    11461 gcaccgctat catagattgc ccttgtcgga tcataaaaaa attcgcaatt tttcatagcg
    11521 tccccacact ttaaccgatt cgcaatttta tatccgcaat tttgcaagct gcccataaaa
    11581 accgattgcc tgccaagccg acactacttg ttaaagatgc ccgataatgc cgcaactctt
    11641 ccgtatttac cgacaaacgc cctatttgct tagagctgtt ttgttttcca caaaacttta
    11701 atccgttgat ttaacaaatt ttttaccaaa ttctttattt gttcggattc cattagcacg
    11761 attttgagca atatctgtca aaaccatttc caccatatcc aaaacaaaac cgccgccgcc
    11821 acggcattgg acaataagat gagtttccac tgcccgtcct tccgcaatgc cgccttcttg
    11881 ggcttcaaag cacccgcata aaaaaacggt gacagcagca tagaagcgat acataagaaa
    11941 atcaatacgc cgtaatagat tttttcagac ggcatttctc ttcctcaata cgccgcccgt
    12001 tggcgcatct gttcaaacag gcagattgtc gccgccatcg cgacatttaa agactcggtt
    12061 gcatcgtgca tcggtatcct gacacacttg tccgccctgt ctaaaactgc tttaccgaca
    12121 cccgcacctt cgttgccgaa cacccaagct gtcggttcgc acaaatcttc gccgtacaaa
    12181 accgcctgct tttcctcgcg caacgcggtg gcaaacacac ggtcttcata gtgcgccaac
    12241 catatttcca aatccgcctg cgaataaatg tccaacaaga aatgcgcgcc catacccgcg
    12301 cgtaatacct tgggcgacca cgcgtccgca caacccctgc ccaaaacgac cgtacctacc
    12361 cctgccgccg ccgcactccg taagacggtg ccgacattgc ccggatcttg cacgccgtcc
    12421 aaaaccacac agtcgccttt gtccggcaag gttccgccca atgggatatc aatcagtgta
    12481 agtatatcgt ctgcacaact caagctgctg atttttttca atatgccgtc tgaaacggaa
    12541 aaaatcccgt cttccggcaa aaccgctgtc aatttaagga cttcttcaga cggcatttta
    12601 gcttcgggaa tatatacccc gaccggcttt ctgccggatt gcaggaaaac ctgaagcagg
    12661 tgcacgccct ccaaaacggt ttgggcatac tgccgtctga actttccttg cgacaacagg
    12721 cggtgcaggt gtctgatatg ttcattattg gtcgaactga tgtgtttcac ttcacaagcc
    12781 ttttagaaat atgcaaccgg acaatacccg actgaacgcc gccgaaatct gtttgcgata
    12841 ttatcaaaat aaaatgggaa agtcaggatg tacgggcaaa atgccgtctg aaaccgagat
    12901 ttgaaaatcc gcacaacagt ttgccaacgc caatggacgg tttccgctgt cggcaacgag
    12961 cggccaaatt gggcgcagga aaggcagaac ccgtttcccc tgaaggattt ttttcacatt
    13021 tttatggatg ccgccgacag ccaacgtatc cgatgccgct accgtcctca aaatcccgtc
    13081 ctgttccaac atggcttcag gaagtccgaa cggatgccgc ttcaacacaa atctatgttc
    13141 tgtcagtatt tttttgagat ttccgcttat cgtcccatct ttcagaaaag ccggttttgc
    13201 caactgttcc gacgcaaaca caaacagcct gcctgcataa tgatgcaatt caaagccttg
    13261 caagttccaa cgtccggttt ttgcctccgt caaaacgcgg gcaatgtcgg caagtgcgtt
    13321 ctgattcggc acggggatgc cgttttcctt tagaaaattc cgcaaaatat gggttttccg
    13381 gcgcggggaa aacgtcagcc accgcgccgt atcgaaataa ccggccccgc aaacccaacg
    13441 gcagtcttga acgacgacct cttccaacaa agccaaatct tcctgcaaag cgcggacatt
    13501 gttcagcaca tgccgcccga aatggggaat ctgcgccgaa agttcgggca aaatacggtg
    13561 ccggaagcgg tttcgcaaat aagccgtatc ggtattgctt tcatcctcga tattcggcaa
    13621 accgtgtttt cgggcataat cccatatgtc ttggcgcgaa aaaggcagca aaggccgcca
    13681 gataatgccg ttttccccca aagggcggac ggcgggcata gccgccaaag cgcgcaaacc
    13741 tccgccgcgc gcgactgcca gcataaaggt ttcgatttga tcgtccctgt ggtgcgccaa
    13801 cgccaaaacg tcaaagcctt tttcggcaaa ctcggcataa cgcttttgcc ttgccgccgc
    13861 ctcgatgccc aaaccgtttt tttccacgca gaccttaacc gtttccagcc ccacccctag
    13921 catatcgcaa taattccggc agaaatccgc ccaatcgtcg gctcggggac tcaagccgtg
    13981 atggatgtgc aatgcctccg gcacaaatcc tgcccgcttc ccggcacaaa ccagcagatg
    14041 cagcaaaacg acggaatcca agccgccgct taatgccact gctgtttttt ttccattcag
    14101 accttgagga aaacaatcct tcaagcattg ctcaaacgca tctaaagtca gcacggcttc
    14161 atcccgataa agaaaaagca agcagtttca gacaaactgc ttgctgcttg ttctacccga
    14221 attattgttc cgaaaatttg ccgtaagcca taatgcggtc gaaacggcgc gaaagcaaat
    14281 cggcaagcgg gatgctttgc gcttcgtgca gttgtttttc caaaacggct tttacgtttt
    14341 tcatggtttg cccgaaatcc ctatgcgcac cacccaaagg ctctttaatg acggtatcga
    14401 ccaagtccag cttttgcagg cggtcggcag taatacccaa agcctgtgcc gcatcagccg
    14461 ccttttcggc ggttttccac aaaatcgaag cacagccttc gggggaaata acggaatagg
    14521 tcgaatattg cagcatattg acgtaatcgc ctacggcgac cgccaacgca ccgcccgaac
    14581 cgccttcgcc gatgacggta cacaaaacgg gaacgcgcag tcgggtcagt tcgtacaggt
    14641 ttttgccgat ggcttccgac tgcccgcgtt cttccgcgcc gatgccggga tacgcgcccg
    14701 gcgtatcgat aaaggtcatg acgggcaagc cgaatttttc cgccgttttc atcaggcgca
    14761 gggctttgcg gtagccttca ggacggggca taccgaagtt gcggcggatt ttttctttgg
    14821 tgtcgcgccc tttctgatgc ccgacgacca caacgctttg tccgttgaaa cgcgccaatc
    14881 cgccgacaat cgcatgatcg tcggcaaagt ggcggtcgcc gtgcagctct tcaaaatcgg
    14941 taaacagcgc gtcaatgtaa tccaaagtat agggacgctg cggatgccgg gaaacctgtg
    15001 aaatttgagc gggtgtgagt ttgctgaaaa tcgatttggt caaatcgttg ctttttttct
    15061 gcaaacggtg tatttcgtcc gaaatatcga cggcagactc gtcttggacg aaacgcagct
    15121 catcgatttt gttggtcagt tcggctatgg gttgttcaaa atccaaaaaa acaggtttca
    15181 taaaatgaag ctctcatcaa cagattctgc cgctatcata cgctatttgt gcatattccg
    15241 cagttaattt attgtccaaa acggcttttc cacaccctct ttttgtgttc ggacagcatg
    15301 gaaaacagcg gattagtgca tatatactag ttataaggcg atgtatctta tgctaaactc
    15361 gaaacaaatt caattatttc aaacaaaatc gtatgaaaga caagcacgat tcttccgcca
    15421 tgcggctgga caaatggctt tgggcggcac gttttttcaa gacccgttcc cttgcgcaaa
    15481 agcatatcga actgggtagg gttcaagtaa acggctcgaa agtcaaaaac agtaaaacca
    15541 tagacatcgg cgatattatc gacctgacgc tcaattccct tccccataaa atcaaggtta
    15601 aaggtttgaa ccaccaacgc cgcccggcac ccgaggcgcg gcttctgtat gaagaagacg
    15661 cgaaaacggc agcattgagg gaagagtgca aacagctcga ccaattcagc cgcatcactt
    15721 ccgcccatcc cgacggcaga ccgaccaagc gcgaccgccg ccaactggac aggctgaaaa
    15781 aaggagactg gtaaaaccat tcccgctgca aacagaagca cgaaccgcgt accgccgcat
    15841 ttcagacggc attcctgaaa cttattccgc ctcgtctatc catgcctgct gcacggcttc
    15901 aaggatgcgt tcgccgcaac gggcgggatc gtcgtcaaat tcgggcaatg ccataatcag
    15961 gtcgcgcagt tgggtaaagc gcacggtttt gggatcgatg gcttcgccgt gcaggtcgta
    16021 gagttcttcg gcgatgcgcc gggtatcggt ccatttcata taaatttcct tttatcgtcc
    16081 ggcattgaag ccgtgtagaa tatgtttcgg caaaactgcc gccccttgag gatggctgaa
    16141 tgtttcaact cgccggacgg cacatcaagc accaccttat gtgtcgtcct gatccgggag
    16201 gagttacccc tcccaaacaa atctgattct accgccctga agagcggggt ttcaaccgat
    16261 aaggaagatt gatgaacaat atgtttgccg caaaattgtc cgaactggtt tataccgctt
    16321 ccgaccatcc cgactccctg tcggagatgg aggagtttga ccgcctgatt ctgctgatac
    16381 gcaaactgta tcaaatattg gacgggcaac ataccctcta cagagtaacg gtttgcctta
    16441 actaccggag caggcgcggt ctgattgctt tggataaagc ggctgtcggt tgcgatgcgg
    16501 caatgttgcg cgcccggcgt tggcgcatct gcatgcagat tgcggaacgc ctttccgagt
    16561 cggtcgggcg gcttcacggt tgcggcagaa agcgtgtccg ccgcctgccc cgagcttgaa
    16621 ggacgctatc tcgaccttgt ccgccgcgcc gccgtctctt tcggtttcgc gcaaggacag
    16681 gcaaatccgg caggcgggca ggaagaaacg caagccgcct cagatgccgt atcgggtcgt
    16741 tccgtcaaaa aaccgataaa aatcaatata gaatcgaaag ttccgtcttc acaaacgtct
    16801 gtttttcggt aattgcgcct tatccgcgca acaatgccgt ctgaagcgtg ccggcttcag
    16861 acggcctgtt ttcagtgttc ttcgcgcgcg tggttgatgg tgtatttggg gatttccaca
    16921 atcaaatcct cgccggcaac aaccgcctga caactcaggc gcgaatcggc ttccaaaccc
    16981 caagcctgat cgagcaggtc ttcttccaat tcggccggct cttccaaact gtcgaaacct
    17041 ttgcggataa tcacgtggca ggtggtgcag gcgcaggatt tttcgcaggc gtgatcgact
    17101 tcgatgtcgt ggtcgagcag cacgtcgagg acggttttgc cttcgggcgc gttatcgatg
    17161 actgcaccct cggggcataa tgttgcgtgt ggaagtacgg tgattttggg catttttatt
    17221 gtctttctgt aaaaagtatt tgtgtccgtt tttaggaaaa ctgtctgcct aatgtcgcgg
    17281 ttcgagcagg tcggcaacat tttgccagcc cgtcaggcgg gcaaaatcga gggcggtata
    17341 gccttccgat gtctttgagg caggatttgc gccgtattca agcatggctt gaaccataag
    17401 tgcatttcct gccatggctt catgatgaag cggcgtatag ccgtcttcgt cctgctcatg
    17461 cagaaaatcc ggattttggg acaactgttg ccgaaaatcg tcaatacagt tccggcacat
    17521 cggatgctct tccagatttt cgggatgttc tttttcttca tacaccggca atatctgtcc
    17581 gggatcgccg aaatcgattc cccatgcggc atcgtgttct gcgcgctcct cttccgtcat
    17641 ctgcccgcgc attgcctgta cggtaaagcc gccgtagggg atgccgttgc acacgaacat
    17701 ccagtcgctg atgtcgccaa ccggaacgca aacactttcg ccttgttcga cattggtcag
    17761 tccgccgggt tcattgttca gcacaccgta aatgtaaaga ccgtcaaaat aaatatcgtc
    17821 gatccacata tgttcgcaaa tttcgccgtc ttcgccgtct tggaaaaaag ggactttgac
    17881 catggcaaaa tccaaggcgg aaataatgcg gcggcgttcc caaaaaagct cgcgccaaaa
    17941 gtatttgaat gttttacggg cgcgttcgcc ggcacggttt accggttcgt ctgcctgttc
    18001 tacataataa atgacggaat cgcccatcat actgctcctc aacgtgtacg gtatctgttt
    18061 gcaccttacc gcggttttct gcctccggca tccgattcgg atttgaaaag ttccaaatat
    18121 tcggaatagg ctttttctaa gtttacccgc gaaaaccagg tgtaaatatg gatgccgtct
    18181 gcaatttcgt cggtcagcct cctgccgctt ttgcgggaat atgcttcttt aaaaaggcgg
    18241 attttcgatt ttttcggctt gtaggatgcc gcgtccgcca aaagccgggg cagcgcgtaa
    18301 gggtattccc ggtgtccgcg ccgtaccaaa tcaagcagtt gtttgcggcc ggacccggca
    18361 ttcccttcct gccggcgttc gtggacgctt ttttcaaatt ccgattccgc cgtttcgttt
    18421 ttcgggaaca cgatttcgcc gcgaagcgat aaaaacaaac gtttcacaag gttttcgtcc
    18481 acggcgaaca attcggtatc gcctatcctg cttttgctga aaatttcatg gagcaggttt
    18541 tttttctttg taattgtcgg ttttgacggc gaggaggcgt tccaacccgg caacgttcgc
    18601 atagccgttg ttttccaaat ggcgcattct gccttcaaaa ttgcttacgt ccgaaatgcc
    18661 gattttatat agtgaattaa caaaaatcag gacaaggcgg cgagccgcag acagtacaaa
    18721 tagtacggca aggcgagcca acgccgtacc ggtttaaatt taattcacta tacacgccct
    18781 tgatgacggt tttcatcgaa tagacaatgc ctgacttttc catatcgatg tttttcaagt
    18841 gttttcgatc cttcagacgg catcaggtta tatctatgcc gtctgaaacc gtttaagtat
    18901 caaatattat cgacactctg acctgtcagc gcacgttgga tgttgcggtt catgcgcttg
    18961 gcggcgaaat tgtcggtgct gcggctgagt ttggcgacag ctgcacggat gtcttcagct
    19021 ttaccgtctt tcagacggcc ttgcaaatcg gcgatgtctc gctggatttg tgcaaactct
    19081 tcggcatcca gcaaatcgct gtccaactcg agggcggcgt tgacggcatc ggtcaggctt
    19141 tcggcttcga ccacggcttc ggcgcgggcg cgggccgcca tatcttcggc ggcgttgccc
    19201 atgctgtctt tgagcatttg ggtgatggtg tcatcgtcca aaccgtagga gggtttgact
    19261 tcgatttgcg cctgtacgcc ggtgctttgt tcttgggcgg aaacggacag caaaccgtcg
    19321 gcatcgattt ggaaggtaac gcggatacgc gccgcacccg ccgccatagg cggaatgccg
    19381 cgcagggtaa atttggcaag gctgcggcag tcggaaacga gctcgcgttc gccttgcacg
    19441 acgtgtatcg tcatcgcggt ctgaccgtct ttgaaggtgg taaagtcctg cgcgcgcgcg
    19501 gtggggatgg tggaattgcg cgggatgatt ttttccgcca agccgccgta ggtttccaaa
    19561 ccgagcgaca agggggtaac gtccaacagc agccattcgc cgtcggcttt gttgcctgcg
    19621 aggacgtttg cctgtatggc tgcgccgagc gcgacgactt cgtcgggatt gaggttgttc
    19681 agcggggttt gtccgaaaaa ggtggcgact gcctgttgga cgtgcggcat acgggtcgaa
    19741 ccgccgacca taaccacgcc tttgacttcg tttttaccga caccggcatc tttcaacgcc
    19801 tgtttgaccg gctcgagcgt tttcatcacc aaatgctgcg tcaggttgtg gaactcggcg
    19861 cgactgatgc ttgtgtcgat tgccataccg tctgaaagcg tcgcctgaat gcgcgcttcg
    19921 gtttgcgtgg tcaattgttc tttggcggcg cggacgagcg agagcaggag ttggctgtct
    19981 tgttcgttga gttgggagag tctgttttgt tcgagcaggc ggcagaacag gcggtggtcg
    20041 aagtcatcgc cgcccaacgc gctgttgccg ccggtggctt tgacttcaaa cagtcctttg
    20101 gtcagttgca acacggatac gtcgaatgtg ccgccgccca aatcgtacac gacaaacgtg
    20161 ccttccgagg cgttgtccag cccgtaggcg attgcggcgg cggtaggttc gttgagcagg
    20221 cgcaatacgt tcaaaccggc cagacgcgcg gcatctttgg tggcctggcg ttgcgcgtcg
    20281 tcgaaatagg cggggacggt aatcaccacg ccgaccaaat cgccgcccaa ggtttcttcg
    20341 gcacgcaatt tgagggtttt gaggatttcc gccgacactt cgacaggcgt tttcaccccc
    20401 tgccgcgtat gcagttcgat aaagcgttga ttgtcgccga aacggtaagg caggtaatgc
    20461 gtattttgat gcagatcggc aagagtccgc ccgataaggc gtttggcgga gctgacggtg
    20521 ttcagcgggt cggttttttg ggcggacagg gcggttttgc cgacttcaat gccgccgttt
    20581 tccagatagc ggacgacgga aggcagggta acgcgcccgt cggcatcggg caggcaggcg
    20641 gcactgccgc tgcggacggt ggcgaccaag ctgttggtcg tacccaaatc gatgcctgcc
    20701 gccaaacggt gccggtgcgg ggcggcggac atacccggtt ctgaaatctg caaaagagcc
    20761 ataaattctg cctgtcggtg cggtgtgtaa aggaaggaag gcggcatttt aacagaattt
    20821 gcgccgatag ttgagcaaac cgctaaggat tgggccgtgc cgccgaaatt gccctctacc
    20881 gccaaatacc gataaaatcc gcctttcccg cccatcatta ggaatataaa tcgtgaaagg
    20941 actcgactat tgccgccaaa aagcagagga aagccgctcc agttttttgt cgggcttccg
    21001 tttcctgact caggaaaaac aggatgcggt aacggttctg tatgcctttt gccgcgaatt
    21061 ggacgatgtg gttgacgaat gttccaaccc cgatgttgca caggcaacat tgaactggtg
    21121 gcgcggcgat ttggacaatg cgttcggcgg cgcgatgccg gaacaccccg tcaatcaggc
    21181 cttgcggcag gttaaggaaa ccttcaagct gccgaaatat gaactggaag ccttaatcga
    21241 cgggatgcag atggatttgg ttcaggcgcg ttacggcagt tttgaggaat tgaaactgta
    21301 ttgccgccgc gtcgcgggcg tggtcggctg cctgattgcg cggattttgg ggttttcaga
    21361 cggcaaaacg ctggaatacg cggataaaat gggtcttgcg ctgcaactga ccaacatcat
    21421 ccgcgatgtc ggcgaagatg cgcgcagggg acggatttac ctgccgatgg aggaaatgca
    21481 gcggtttgac gtacccgcaa gcgtgatttt gcaatgcagc ccgacgggca attttgccga
    21541 attgatggcg ttccaaatca aacgcgcccg tgaaacctac cgcgaagccg tatcgctgct
    21601 gcctgatgcc gataaaaaag cccaaaaagt cggactggtt atggcggcgg tttattacgc
    21661 gctgttgaac gaaatcgacc gagacggcgc gcaaaacgtc ctcaaataca aaatcgccct
    21721 cccttcgccg cgcaaaaaac gcattgccct gaaaacctgg ttattcggat tcaaaccgcg
    21781 ccccggcacg ccggaacggg cataaggcgc atactgccct tcaggaaaac ccaacccccg
    21841 ccgccccgaa aaacggggtt tcaaccattg gaggaataaa tgatgaacac gccgcatccg
    21901 cgcccgaaaa tcgccgtcat cggcgcaggc tgggccggct tgtccgccgc cgtcaccttg
    21961 gcgcggcacg ccgacgttac cctgtttgaa gccggccggc aggcgggcgg aagggcgcgc
    22021 acactggccg gaaataccga cggtttcggt tttttggaca acgggcagca cattttgctc
    22081 ggcgcatacc ggggcgtgtt gcgcctgatg aaaaccatcg gttcagaccc ccgtgccgcc
    22141 tttttgcgcg taccgctgca ctggcatatg cacggcggtt tgcagttccg cgccctcccc
    22201 ctgcccgcgc cgctgcatat tttgggcggc gtgctgcttg cccggcgcgt accgtccgca
    22261 ttcaaagcca aactgcttgc cgatatgtcc gatttgcaga agtccgcacg cctcggacag
    22321 cccgacacga cagttgcaca atggctgaaa cagcggaacg tgccgcgtgc cgccgtaatg
    22381 cagttttggc agcccttggt ctggggcgcg ctcaacacgc ctttggaaac cgcaagcctg
    22441 cgcgtgttgt gcaacgtttt gtccgacggc gtgctgacga aaaaatccgg cagcgactat
    22501 ctcctaccca aacaggattt gggcgcaatc gtcgccgaac ccgccttggc ggagcttcaa
    22561 cggctcggcg cggacatccg cctcgaaacg cgcgtatgcc gtctgaacac cctcccggac
    22621 ggaaaagtcc tcgtcaacgg cgaagccttc gatgccgcca tacttgccac cgcgccctac
    22681 cacgccgccg cgctcctgcc cgaaggcacg cccgaacacg ttcagacggc atatcaaaac
    22741 cttcgctacc acgccatcac caccgtctat ctgcgctacg ccgaacccgt ccgcctgccc
    22801 gccccgctga ccggcattgc cgacggcacg gcacaatggc tgctttgccg gggcaggctc
    22861 ggactgcccg aaaacgaagt ctccgccgtc attagcgttt ccgaccgcgt cggcgcgttt
    22921 gcaaaccggg cgtgggcgga caaagcccac gccgacctca gacgcatcct tccgcatttg
    22981 ggcgaacccg aagccgtgcg cgtcatcacc gaaaaacgcg ccacaaccgc aaccgatgcc
    23041 ccgccgccgg atttgtcgtg gctgcaccgg caccgtatct tccccgccgg cgactacctc
    23101 cacccgcact accccgccac gctcgaagcc gccgtacaat caggtttcgc gtcggcggaa
    23161 gcctgcctgc aaagcctgaa cgatgccgtc tgaaaacgcc ggccggcatc gggacgcttt
    23221 acaaggtgcg gcaaaacgct aaaatacatt aaccgtcaaa cccaaaggac aaccatgccg
    23281 acactgaccg acaaaaccat cttagttacc ggcgcatcgc aaggcttggg cgaacaggtc
    23341 gccaaagcct acgccgccga aggggcgacc gtaattctgg tggcacgcca tcagaaaaaa
    23401 ctggaaaaag cctatgacgc gattgtcgaa gccggacacc ccgaaccctt cgccatccgt
    23461 ttcgacctga tgagtgcgga agaaaaaaaa ttcgaacggt ttgccgccac cattgccgaa
    23521 gccacgcaag gcaaactgga cggcatcgtc cactgcgccg gctattttta cgccctctcg
    23581 ccgctggatt tccaaaccgt cgccgaatgg gtcaaccaat accgcatcaa caccgtcgcc
    23641 ccgatggggc tgacccgcgc cctgttcccg ctgctgaagc agtcgcccga cgcgtccgtc
    23701 atcttcgtcg gcgaaagcca cggcgaaaca cccaaagcct actggggcgg cttcggcgcg
    23761 tccaaagccg cgttgaacta cctgtgcaaa gtcgccgccg acgaatggga acgcttcggc
    23821 aacctgcgcg ccaacgtcct cgtccccggc cccatcaatt ccccgcaacg catcaaatcc
    23881 catccgggcg aagccggaag cgaacgcaaa agctacgggg acgtactgcc cgcatttgtc
    23941 tggtgggcaa gtgccgaaag caaagggcgg agcggcgaaa tcgtttacct ctaaatccgg
    24001 aaaaggcagg catcccgccc gcgccgcgct tccgttttga aaaatatccg taccttccgc
    24061 gcgcatccgt cccgcacaga caaacacctt gacttaaatt tacataagga caataatgat
    24121 aacccgttat ttccctaact ggaccttagg gtaaatttta tgaaaaccgt ttccgccgcc
    24181 atcgcttttg ccgccgctgc cgtttcactg accggctgtg cgaccgagtc ctcacgcagc
    24241 ctcgaggttg caaaagtcgc ctcctgcaat acgcaatatc acggtgttcg caccccgatt
    24301 tccgtcggaa cattcgacaa ccgctccagc ttccaaaaag gcattttctc cgacagtgaa
    24361 gaccgtctgg gcagccaggc aaaaaccatc ctggtaacac acctgcaaca aaccaaccgc
    24421 ttcaacgtac tgaaccgcac caaccttagc gcattgaaac aggaatccgg catttccggc
    24481 aaagcgcaga acctgaaagg cgcagattat gtcgttaccg gcgatgtaac cgaattcgga
    24541 cgcagagatg tcggcgatca tcagctcttc ggcattttgg gtcgcggcaa atcgcaaatc
    24601 gcctatgcaa aagtggctct gaatatcgtc aacgtcaata cttccgaaat cgtctattcc
    24661 acacagggcg cgggcgaata cgcactttcc aaccgcgaaa tcatcggttt cggcggcact
    24721 tccggctacg atgcgacttt gaacggcaaa gttttagact tggcaatccg cgaagccgtc
    24781 gacaacttgg ttcaggctgt cgacaacggc gcatggcaat ccaaccgtta aggagaaatg
    24841 atgaatccga aaaccttggg ccgtttgtcg ctgtgtgcgg cggtcttggc tctgaccgcc
    24901 tgcggcggcg ggcataaaaa cctgtattat tacggcggtt atcccgatac cgtctatgaa
    24961 ggtttgaaaa acgacgacac ttcgttgggc aagcagaccg aaaagatgga aaaatacttt
    25021 gcggaagccg ccaacaaaaa aaatgaatgc cgccccgggt gcgcacgccc atttgggact
    25081 gctgctttcc cgttcgggag acaaagaggg cgcgttccgc caatttgaag aagagaaaag
    25141 gctgtttccc gaatcgggcg tatttatgga cttcctgatg aaaaccggta aaggaggcaa
    25201 gcgatgaaac ctttgatttt agggcttgcc gccgtgttgg ctctgtctgc ctgccaagtt
    25261 cgaaaagctc ccgacctcga ctacacgtca ttcaaagaaa gcaaaccggc ttcaattttg
    25321 gtggttccgc cgctgaacga gtcgcctgat gtcaacggca cttgggggat gctggcttcg
    25381 accgccgcgc cgatttccga agccggctat tacgtcttcc ccgccgcagt cgtggaggaa
    25441 accttcaaag aaaacggctt gaccaatgcc gccgatattc acgccgtccg gccggaaaaa
    25501 ctgcatcaaa ttttcggcaa tgatgcggtt ttgtacatta cggttaccga atacggcact
    25561 tcatatcaaa ttttagacag cgtgacgacc gtatccgcca aagcacggct ggtcgattcc
    25621 cgcaacggga aagagttgtg gtcgggttcg gccagcatcc gcgaaggcag caacaacagc
    25681 aacagcggcc tgttgggggc tttggtcggc gcagtggtca atcagattgc caacagcctg
    25741 accgaccgcg gttatcaggt ttccaaaacc gccgcataca acctactgtc gccctattcc
    25801 cgcaacggta tcttgaaagg tccgagattc gtcgaagagc agcccaaata agcatatgcc
    25861 gtctgaacct ctccgagctt cagacggcat atgcgaatct tgatacttgc aagccgcacc
    25921 gcccaaaatc aggcgaccgg tttcaatatc cgaacaaggt tcgggttctg tattttgcag
    25981 aacccgaacc ttttattcaa atttaaactt aattccggcg tggttgtatg gtaaattata
    26041 gtggattaaa tttaaaccag tacggcgttg cctcgccttg ccgtactatc tgtactgtct
    26101 gcggcttcgt cgccttgtcc tgatttttgt taatccacta taacaagcaa tatgccctga
    26161 ccggcaaaac ttccccaaac ttcatcaaaa aatcaactgt acccgtcttt ttcagacggc
    26221 attgataccg taagagattt cccatgcaaa accggcaaac cgagctttat tccacgccgt
    26281 cttggctgtt gcagacctta ttgatgatta ccgccgcctc ggcggtgatt ctgtttttcg
    26341 cgcgcaacac gcgcttgggg cgggagtttg cctatatcct gcgcctttgc ctgacaccga
    26401 aaagcacggt caaagtattg ctgctgatta cggcaatggt tacgctgctg ttaaccgaag
    26461 tgcggttgaa tgtgttgagt accttcatgt ccaaaggact ttacgattcg atgcaggatt
    26521 tgaatgcttc ggcattttgg atgtttgcag cgatgaacgc cggcgtggta ctgatacggg
    26581 cgtttaacaa cgtcgttaac gactttctcg attaaggctt ggcgattaaa tggtcggaac
    26641 ggctcaatga agttttgact tcgcgctggc ttgccgacaa aaactactac cgcctgcaaa
    26701 tgcgccgcca cgcgccggac aacatcgacc agcgtatcca acaggatgcg caggaattca
    26761 tcgcttcgac catcgaattt gtgcgcggca tggtcaattc ggtcgttact tctctggaat
    26821 ttgccgttgt tttgtagggc ttggcaggca tcctgaccgt gttcggcttc gacattccgc
    26881 acggcattgt ttggtttgtc tttattttca tgattttggc gatctttatc gccatgtgga
    26941 taggcaaccc tttgattcgt tacaattatg aaaacaaaaa actcaacggc gactaccgtt
    27001 attccctcat cctcgtacgc gaccacgccg aaagcgtggc gttttacagc ggcgaacaac
    27061 acgaacacgg ccggctttcc gaccgcttta aagccattat ccgcaaccgt tggcgcatcg
    27121 cgcggcaaag cgtctgctta agcggcttta acgatatgtt caccaacggc atcaaactct
    27181 tcctgattat tttgcaagct ccgcgcctgt ttgccggaca aatcaaaatc ggcgacatcc
    27241 agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct gtctttcttc cgaatgttct
    27301 acaacaaatt caccgcctgc cacgcccgac tggagcgtct gtacgtcttt ttgctgagta
    27361 ccgaagaaca acacggcgcg cagcaacccg agattagcga agtttcagac ggcattgcac
    27421 tggaaaacgt cgccctgttc cgccacaacg gcgaagtttt attggacggc atcaacatca
    27481 aactcaaaag cggcgattcc ctgctgatac gcggcccgag cggttgaggc aaaacctcgc
    27541 tgctgcgcgc gctggcgggg ctttggccgt tcggcagcag cggcaaagtc agccgtccgc
    27601 cgcatcaagg catcctcttc ctgccgcaac gcccgtacac ggcacaaggc agcctgcgcg
    27661 acgcggtttg ttaccccgat atcgacaaac agcatcccga gttagccgaa gccatgaaca
    27721 cctgccgctt gggttatctg attgacaaat tggataaaac agacggctgg cagcacaaac
    27781 tctcccccgg cgaactgcaa cgcgtcgcct tcgtccgcgc cctgctctcc aagcccaaaa
    27841 tcgtcctgct cgacgaagcc gccgccgcct tggacgaacc ggccgaagcc tagctctacc
    27901 gcgccttaaa gcaaaaactg cccgacagca tcatcagtat cggacaccga agtacgctca
    27961 acgcgttccg caatatgcac ctcgatgtcg gcaacgctgc ctgcggctga acgatgccgt
    28021 ctgaagccgt tttcagacgg catcgttcaa attggaaaga cacgcgaaaa caacgtacaa
    28081 taaacccgat tccaacccat ccggtatgcc tttgtgaacc agcttatttt cgactttgcc
    28141 gcacacgact atccgagttt cgacaaattc ctcggcacgg aaaatgccga actggtctat
    28201 gtgttacagc ataaacacga cccgtttatc tacgtctggg gcgaagaagg cgcgggcaaa
    28261 agccatctgt tgcaggcgtg ggtcgcacag gcgctcgaag ccggcaaaaa cgccgcctat
    28321 atcgatgccg cctccatgcc gctgaccgat gccgcgtttg aggcagaata cctcgccgtc
    28381 gatcaagtcg aaaaactggg caacgaagaa caagccctgc ttttttccat cttcaaccgc
    28441 ttccgcaaca gcggcaaagg ctttttgctg ctcggttcgg aatacacgcc ccagcagctc
    28501 gtcatccgcg aagatttgcg gacgcgtatg gcatactgcc tcgtttacga agtcaagcca
    28561 ctaaccgacc aagaaaaaat cgacgcgctc gccaatatgg cggcggcacg ccaagtaacc
    28621 gtcgattccg aaattttcga atacctgctc aaacactggc ggcgcgatat ggacagcctg
    28681 atgatgatgc tcgacacgct ggacaactac gccgtaacca tgggcaaacg catcaccctg
    28741 ccgctgcttc gccagctttt gaaacaacag gaaacccaat gaaaaacctc gccatcttcg
    28801 acctcgacaa caccctcatc aacaccgatt ccgaccacgc ctggccgcaa tacctcatca
    28861 aaaaagggtt ggttgacgct gccgaaaccg aagcgcaaaa cgaaaaattc taccgcgact
    28921 accaaaacgg ctgcctcgac atcgacgcat tcctcaaact ccacctcgcc ccgctcgccc
    28981 gttacagcaa agaagagctg gcggagtttc accgcgaatt tatggcggaa ttcatcacgc
    29041 cccacatctc gcctatgcag catatgctgg tgcagagcca ccaaatggca ggcgacgaaa
    29101 ccctcgtgat ttcctcaacc aacgagttca tcatcacccc cgtctgccgc cttttcggca
    29161 tcgccaacat gatcggcaca caactcgaga ccggtcccga cggccgctac accggcaatt
    29221 acatcggcac gcccagcctc aaagaaggca aaatcacccg tctgaaccaa tggctcgccg
    29281 aacgcggcga aacgctcgaa agctacggca aaacctattt ttacagcgac tccaaaaacg
    29341 acctgccgct gctgcgcctc gtcagcgaac ccgtcgccgt caaccccgat gccgaactgg
    29401 aaaaagaagc caaagaaaaa ggctggccgg ttttgaattt caaatgacgc aatgccgtct
    29461 gaagcccgat tcggcgttca gacggcattg aggctgaaat cccaaaaacc caaagccaca
    29521 ggaattcatc gggaaaaaca acaatctttc caccgtcatt cccacgaaag tgggaatctt
    29581 gatccgtccg cacggaaact tatatttcgt cattccacca atcccacacc acccttaccg
    29641 gttcaaatcc gcacaaaaat ccacatcccg tcattcccac gaaagtggga atctaggacg
    29701 taaaatctaa agaaaccttt ttttcccgat aagtttccgt gccgacaggt ctggattccc
    29761 gcctgcgtgg gaatgacgaa atttcaaagt tatggcgtta tcggaaaaac agaaaatcaa
    29821 agccggagaa tttatccaaa acaaccggat ttcaaaaaaa ccaaattccc ggcgggaatg
    29881 acggatttta ggtttctgtt gtggtttctg ttttctcggg aataacaact tagaaattgc
    29941 ccgaaaccca aaaagcagaa accggcttcc cgccaaagtc ggcattttcc gactttgctt
    30001 caggcatctt cccctctgcc taaaatcatc gacccgatac cggcatcggt aaagatttcc
    30061 agcaaaagcg cgttgggcaa cctgccgtcg atgatgtgcg tggctttcac accgttggcg
    30121 gcggcttcga ccgcagaagc gattttcggc agcataccgc catacagcgt gccgtcggca
    30181 atcagcccat caatccgttt cggcgtgagt ttggtcagca gattgcccgt tttgtccatc
    30241 acaccggcga tattcgtcat catcaagagt ttttcggcgt tcaattcttc cgccaatttg
    30301 cctgccacca aatcggcgtt gatgttgaac gcttcgcctt tttcacctac gccgacgggg
    30361 gcgacgacgg gaatgcagcc gcgttctatc agccctttaa ccaaaccggt atcgatgctt
    30421 tccaccgtac cgacctgtcc gatgtccacg ctattctgtt cgggcgtatc gaccaaaagt
    30481 ttcttcgcct taatgaaatg gtcgtcgcgc ccgctcacgc cgaccgcgtg ccctccatat
    30541 gtgttaatca tcgacacgat ttccttgttg acgtgcccgc ccaataccat ttcgacaata
    30601 tccatcgtct ctttgtcggt aacgcgcatt ccttggacaa attcgccctt tttgccgact
    30661 ttttcaagca tcgcattgat ctgcggtccg ccgccgtgaa cgatgacggg atgaatgccg
    30721 accagcttca gcagcacgac attgcgggca aacccttctt tcaaggcagg ttcggtcatc
    30781 gcgttgccgc catacttgat gacggcgacc gaaccggaaa atcggcggat ataaggcagt
    30841 gcttcggcaa ggatacgcgc cttgtcggcg gcggaaataa tgttttcaaa ctccataacg
    30901 gctccatatt gttgacaatt tcggcttatg ttaacgccaa acgccgtctg aaacaatctt
    30961 cagacggcgt ttggcacacc ttcgggcagg ttgaagcctt gcgatacggg cggtaaaatc
    31021 gcggtttatc cggttcgaga aaccggcgac agcaaggact ttcccatgat taaaatcagc
    31081 acccgcttcg atgccggctc ggtcgtcgtc aaagacctga ccgacccttc caacatccgc
    31141 ctcgccctgc gtcccgacaa cgcctccgat ttcgcacaat ggttctactt ccggctgcaa
    31201 ggcgcggcct atcaaaactg catcatgcat tttgaaaacg cggcagaagc cgcctacccg
    31261 aaaggctggg aaggctatca ggcgtgtgcc tcatacgacc gccgcaactg gttccgcgta
    31321 ccgacttcct acgaaaacgg cgtactgacc gtcaatcata cgccgctgtc caacagcgtg
    31381 tattacgcct atttcgaacc ctattccgaa gagcagcacc tcaacctcct cggcgacgcg
    31441 caaggcagcg gcctgtgccg catcgacgac ttgggcagca ccgtgcaagg ccgcgacatc
    31501 aatctgctga ccataggcaa ccaagtcgaa agcgacatga aaatctggat taccgcccgc
    31561 cagcacccgg gagaaaccat ggcggaatgg tttgtcgaag ggctgctcgg caggctgctc
    31621 gattcgcaag acccgacagc acgcaccctg ctcgaccgcg ccacgttcta catcgtcccc
    31681 aatatgaatc ccgacggttc ggcactgggc aaccttcgta ccaacgccgc cggtgcgaac
    31741 ctcaaccgcg aatgggaaaa cccgactttg gaaaaaagcc ccgaagtgtt cttcgtgcgc
    31801 ggaaaaatgc tggaaaccgg cgtggatttg tttttggaca tccacggcga tgaaggtctg
    31861 ccctttgtct ttgttgcagg aacggaaggt gtgccgaact acaatccgcg catttccgcg
    31921 ttggaagcgc agttcaaaac cgcccttttg aacgccagcc ccgatttcca agacgaatac
    31981 ggttacgaaa aagacgcgcc gggcaaagcg aatatgacct tggcgaccaa ctgggtcggc
    32041 aaccgtttca actgccttgc ctacacgctg gaaatgccgt tcaaagacaa cgccaacctg
    32101 cccgacgacg acttcggctg gaacggccag cgttctttgc ggcttgggga agcggtgctt
    32161 tccgccattt tgaacgtcgc cggcgatttg cgctgacact ccccactgaa aatgccgtct
    32221 gaaagccctt ttgcgtcttt cagacggcat ttgaaaacga aagcccgtta tgattatcca
    32281 tcaccaattc gatcccgtcc tcatcagtat cggcccgctt gccgtgcgct ggtatgcctt
    32341 aagctacatc ctcggattta ttctttttac ctttctcggc agaaggcgca tcgcgcaagg
    32401 cttgtccgtt tttaccaaag aatcgctcga cgacttcctg acatggggca ttttgggcgt
    32461 gattttgggc ggacgcttgg gctatgtcct gttttacaaa ttctccgact acctcgccca
    32521 tccgcttgat attttcaagg tatgggaagg cggaatgtcg ttccacggcg gctttttggg
    32581 tgtagttatt gccatatggt tgttcagccg caagcacggc atcggcttcc tcaaactgat
    32641 ggacacggtc gcgccgctcg ttccgctggg tctcgcttcg ggacgtatcg gcaactttat
    32701 caacggcgaa ctttggggac gcattaccga cattaacgca ttttgggcaa tgggcttccc
    32761 gcaagcgcat tacgaagatg ccgaagccgc cgcgcacaat ccgctttggg cagaatggct
    32821 gcaacaatac ggtatgctgc cgcgtcatcc ctcgcagctt tatcagtttg cccttgaagg
    32881 catctgcctg ttcgccgtcg tttggctgtt ttccaaaaaa ccgcgcccga ccgggcagac
    32941 tgccgcgctt tttctcggcg gctacggcgt gttccgcttt attgccgaat ttgcgcgcca
    33001 acccgacgac tatctcgggc tgctgacctt ggggctgtcg atggggcaat ggttgagcgt
    33061 cccgatgatt gttttgggta tcgtcggctt tgtccggttc ggcatgaaaa aacagcactg
    33121 accgcctgtg aaaaataccg tctgaaacat ggaaatccgg tttcagacgg catttttacg
    33181 tttggttcag atgaaaacaa acgcttcaat tcttaccgca acacgccttg tatttcctgc
    33241 cgctgccgca cggacaggga tcgttcctgc cggttttttc cccttccctg cggacggttt
    33301 gcggtttgtt gatgaccgcc tgccagtagc ggtagatgtc tgccagcgcg taaggcagtt
    33361 cggacgcaag ttccgccagc tcgccttcgg tgaattgcag gcggatagcg ccgttttcct
    33421 cttcgtcgta aatgccgccc aatgccatca cggggtaaaa cagctcttca aacgcttcat
    33481 catcggcggc ttcaaaccaa tcggtcggca caatgtccaa accgtaaaga taggcgttgc
    33541 accaagtgta aaaatcgctg ccgccctcgc cgtcgtcgta gagccacaaa tcgggcagct
    33601 ttttatccga catcgcggcg gttgtttcca tcgccattgc caaaaccagc cgttcgattt
    33661 cggaacgttc ggcggcggta aattgcgatt cgtcgcccaa cacttcgggc agccagtcga
    33721 gcggtgccaa tttgtccggc ccgctcaaca gcgccgtcat aaaaccttga acctcgtcgc
    33781 aacgcatcgt gttgccttgt tcgcttttgg catccaacaa ttcgcccaac cgccgtttgg
    33841 atgcttcggt aaattttcgg gaatccatca ttttcctttt caaatgggtt ttgcgcccta
    33901 ttatagtgga ttaaatttaa atcaggacaa ggcggcgagc cgcagacagt acaaatagta
    33961 cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc cactatatcg ccgcaatgcc
    34021 gtctgaagcc tttttatgct tcagacggca tcgtctgttt tcacgctcaa atcgtaccgc
    34081 tgccctgcgc cttcggtttg gcggcactaa attccagctt gctcaaagcg gaaaggtaag
    34141 ctttggcggt cgccgccaaa atgtcggtat ccgcgccctg accgttggcg acgcggttgc
    34201 cgcgcgccaa acggacgctg gtttcgcctt ggctttccgt accttgcgtg acggcgttga
    34261 cggaataaat ctgcaaagtc gcgccgcttt gcgccacgct ctcaatcgct ttgaaaattg
    34321 catcaacagg gccggagccg gttgcggaag cgcgtttttc ttcgcctttg atgccgaaca
    34381 cgatgtcggc gcgcggctct tcgccggttt cggtgctgat tttttgagaa atgaatttat
    34441 agctttcggc attcatattg cccatttcgt ccgacaccag cgcgtgcagg tcttcatcga
    34501 agatttcgcg ttttttgtcg gcgagttctt tgaagcgtgc aaatgccgcg ttcagtgcct
    34561 cttcgctttc caactcgatg cccaaatccg ccagcttggt tttgaaggcg ttgcggccgg
    34621 acaatttgcc caagctcaat cggtttgccg accaaccgac cgattcggca gacataatct
    34681 cgtaagtttc gcggtgtttc agcaccccgt cctgatggat gcccgattcg tgcgaaaagg
    34741 cattcgcgcc gacaatggct ttattgggct gcacgggata gcccgtaatg gtggacacca
    34801 gtttggacga aggcacgatt tgcgtggtat cgatgccggt ttccaagccg aacaagtcgt
    34861 ggcgcacctt caacgccatc acgatttctt caaccgaagc attccccgcg cgttcgccca
    34921 aaccgttaac ggtacattcc acctgacgcg cgccgccttt taatgcggca agcgaattgg
    34981 caaccgccaa gcccaaatcg ttgtggcagt gtgccgacca gacgactttg ccgccgttgg
    35041 gcgttttggc aatcagttcg cggaaaaatt cttcggtttt gtacgggatg gaatagccga
    35101 cggtatcggg aatattgatg gtggtcgcgc ccgcttcaat caccgcgccg cagatttcgg
    35161 caaggaaatc gatttgcgaa cgcaacgcgt cttcgcagga aaattccaca tcgtcggtgt
    35221 attcacgagc gatttttact gctttgaccg ccgcctcaat cacctgcttc ggcttcatct
    35281 tcagcttgta ctccatatgg atggggctgg tggcgatgaa ggtgtggatg cgttttttcg
    35341 gcgcgggcgc aacggcctta cccgcctgac ggatgtcccg ctcgatggcg cgggacaatg
    35401 aacagaccgt tgatttggta atggttttcg caatcgcatt gaccgcctcg aaatcgcccg
    35461 ggctggcggc ggcaaaaccc gcttcgatga tgtccgcgcc caatttttcc aactggcggg
    35521 cgacgcggat tttttcctct ttggtcatag cggcgccggg cgattgttcg ccgtcgcgca
    35581 gggtggtgtc gaaaataata acgcggttgg cctgtgtcat ttctgttctc tccagagatt
    35641 cgtttttttg taaaaaagca ttcaaatcca gcggtcttcc ttgcgattcg gcaagcgcgg
    35701 tcaatttttg cagttgcgcc aaaggcagcg agccgcgtgt gcgccatttg tagatggcgg
    35761 cggtcgtcgc ggcattttca ggatcgtgcc gtttcaacgc ttcggcaagc gcgttcacgc
    35821 cgccgaaata ggcgaccaga cggtctatat ctaattgcat ggtagtcctt gtctaaaata
    35881 aatcagcccg cagcagaaat atggacagga ttatacgcat tttggacaaa acggcaatat
    35941 taatttgaaa aaatctcaat aagttgctat aaattttatt aattttatac attttgtcta
    36001 ttttattgca gatgcggcgt gattgttgac aattgcaaca gaacagggca aattcggcgg
    36061 tacaaggcgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa atacaaaaga
    36121 atcgtattta aagtcggcac atcttcgatt acccgttcgg acggcagcct ctcgcgcggc
    36181 aaaatccaaa ccatcacccg ccagcttgcc gcattgcatc atgcgggaca cgagctggtc
    36241 ttggtgtctt ccggcgcggt tgctgcaggg ttcggcgcgc tgggtttcaa aaaacgtccg
    36301 gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct gatggaagaa
    36361 tatacggcaa acctgtcttc agacggcatc gtgtccgcac aaatcctgct cagccgtgcc
    36421 gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt gctgctgcaa
    36481 cgccgcgcga ttcccatcat caatgaaaac gacacggttt cggttgagga gttgaaaatc
    36541 ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga cctcttggtg
    36601 ctgctgaccg acatagacgg tctttacacc ggcaacccga acagcaatcc cgatgccgta
    36661 cggctggaca aaatcgaaca catcaaccat gaaatcatca aaatggcggg cggctcgggt
    36721 tcggcaaacg gcacgggcgg tatgctgacc aaaatcaaag cggcaaccat cgccgccgaa
    36781 tccggcgtac cggtgtatat ctgttcctca ctcaaacccg attcattggc cgaagccgcc
    36841 gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg gacacagaag
    36901 caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga aggtgcggaa
    36961 cacgctttgt ccgaacaagg gaaaagcctg ctgatgtcgg gcattgccgg aatcgaaggg
    37021 catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca gcccctgggc
    37081 aaagggcgcg tccttttcgg ctccgccgcc gccgaagacc tgctcaaatc gcgtaaggcg
    37141 aaaggcgtgt tcatccatcg ggacgattgg atttccatca cgcccgaaat acgcctgctt
    37201 ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct tgcccttgcc
    37261 aaagcggcaa aaaaatccgt caacacggcg gatgcggaag aaaaaaaccg cgcgctgctt
    37321 gctatggcgg acagtctgga agcggcggcg gaagatattt tggcggcaaa ccgtttggat
    37381 ttgaaagcgg cggcaggcaa aattcccgac agcatgaccg accgcctttt gttggacggc
    37441 aaacgcattt gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc cgaccccgtg
    37501 ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa aaagcgcgtg
    37561 gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacttc cgatgcggcg
    37621 gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga tgcatttcaa
    37681 tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg catcgacccc
    37741 gaagccgtac agctcatcga agacacaggg cgtgagggca gttacgaaat gatgagggcg
    37801 aaagattatc tagacctgct gattccgcgc ggcggggcag ggctgatacg ggcggtggtt
    37861 gaaaatgccg tcgtgccggt cattgaaacg gggacgggca tcgtccacat ttatatcgac
    37921 aaggacgcgg attgggacaa ggcactccgt atcgtgtaca acgccaaaac cggccgcccg
    37981 tccgtgtgca actcgatgga agtgctgctg gtgcatgaag gcattgctgc cgacttcctg
    38041 cccaagctcg aacggctgtt ggttcgcggc cgtatagaag ccggactgcc gcccgtccgc
    38101 ttccgtttgg atccgcaggc ggcgcggtat atcggcggcg aagcggcggg tgcagacgat
    38161 ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcttc ggttgaagag
    38221 gcggtcgggc acatcgaagc ccgcggcacg caccattcag acggcatcgt taccgaaaac
    38281 cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta tatcaacgcg
    38341 tccacgcgct tcaccgacgg cggcgaattc ggcttgggtt gcgaaatggg catctccacg
    38401 caaaaactcc acgcccgggg cccgatgggt ttgaaagagc tgacgagtta caaatacatc
    38461 gtacaaggca cgggacaggt tagggaataa tccgaattca aacgtctgat atagtggatt
    38521 aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
    38581 cgccgccttg tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acagtcttca
    38641 gacggcattt ctatttgcaa aaaaatcaag cattgtccaa ggggacgaga atcgtacggt
    38701 tgccgttgtg ttccggtgcg gacacaatgc cttccgcctc catttggtca atcagacgcg
    38761 cggcgcggtt gtagccgata cgcaaggcgc gctgtacgcc cgaaatgctg gctttgcgcg
    38821 ttttcaggac aacggatacg gcctcgtcgt acatcggatc ggtttcgccg tcgccgctgc
    38881 gcccgatgcc gggcagctct tcgctgccgc cgccgctcaa aatatcgtca acatagtccg
    38941 gctcgccaaa ctgcttcaga tattcgacca cgcggtgcac ctcttcatcc gaggcaaacg
    39001 cgccgtgaac gcgctgcgga taggcagtac ccggcggcag gaacagcata tcgccctgac
    39061 cgagcaggtt ttccgcgccc atttggtcga gaatcgtgcg gctgtcgatt ttgctggaca
    39121 cttggaacgc gatacgcgtc gggatgttcg ccttaatcag acccgtgatg acatcgacgc
    39181 tggggcgttg tgtggcaagg ataaggtgga tgcctgccgc gcgggctttt tgggcgaggc
    39241 gcgcaatcag ttcttcgatt ttcttgcctg ccgtcatcat caaatcggca aactcatcga
    39301 ccacgaccac gataaacggc agtttttcca aaggttcggg atcgtcgggc gtgaggctga
    39361 acggattgcc gattttttct ccccttgctg cggcttcggc ggttttttgg ttgaagcccg
    39421 caagattgcg cacgcccata aagctcatca gccggtagcg tttttccatt tcgttaacac
    39481 accagttcag cgcgtttgcc gccagcttca tatcggtaac gacaggggcg agcaggtgcg
    39541 tgatgccttc gtaaatgctc aattccagca ttttcggatc gatcataatc atacgcacgt
    39601 cttccggcgc ggctttgaaa agcatagaca gaatcatcgc gttgacaccc accgatttgc
    39661 ccgaaccggt cgtgccggca accagcaaat gcggtgcttt gcccaagtcg gttacgacgg
    39721 gctgtccggt gatgtcctga ccgagcgcga gcgtcagctt ggatttggat tcggcaaact
    39781 cgggcgaatt gaaaatttcg ctcaggcgta tcatttggcg tttcgggttc ggaagttcca
    39841 aacccatgca ggttttgccg gggatggttt cgacaacgcg gatggaagcc acgccgagcg
    39901 aacgcgccaa gtctttttcc aaattcagaa cggaattgcc gcgcacgccg acatcgggtt
    39961 cgatttcata acgcgtaatc acggggccgg aataagaatc gacaaccttg accttgactt
    40021 tgaactccgc caatttttct tcgatggtga tgctgttttc caacagttct tcttcggttt
    40081 gcgtcgcctc gggattgaac agcggaggca gaagcaggtc ggttgtcggc aggtgttcgg
    40141 ataccgcacc ggtctcttcc gattggaacg ccccttcatc cgcttccgta tccgataccc
    40201 ggcaggacgg gcgttcagac ggcacatctt caaacggaca aaccgcctga ctgtcatgcc
    40261 cgaacgcttc ggtttccgac agatattgcc cgcttgaccg ctctgccgca ccctcactgc
    40321 cgtcatctgc aatagcggcg gtttcctcct gccaacctcc attcaaaaca tcagcggcaa
    40381 gatggtcggt ttcggcaatg cggctgcgtt gcgcctgctc gaatcctgcc ggcggctcat
    40441 aggtacggtt gtagatttcc gatacgggag gcggcggcag aatatcgatt tcgggtacgg
    40501 ctacctccgg cacttcgggt ggttcgacca cccaagcatc gggcgcggca ggctcttcaa
    40561 tatggatatc ggcagcttca tcataaaccg gaccgccgta tcccacagac gaaacggttt
    40621 ccgtgaaaac attttcggtt tcagacggca tttcgacatc ccgtttcccg acaaccgtcc
    40681 gactttccgg aatcaggcgc gaaatccgcg cctccgcaga aaccgttgcc ttgttcggtt
    40741 ctttcaaatt gacggaaacg cggcggcggg catcgcggac ggctgtgccg tctgaaacgg
    40801 actgcccttg ggaaacatcc ggggattttg cctccgcctg ccctttctcc tgccctgcat
    40861 gacggcggaa cggcgcattc cgtgcatttt cggctttgac tgcagaaaag gcagacggat
    40921 gctgcttctc aaaatacggc tcaaatccgt aatccgcaga ctcggaaaac gcttctttgt
    40981 ccgcatcaaa catacgggaa tacgtgcgtt gaagcacagg gtcgtccaaa ccgataatct
    41041 gcagcccttc catcggggta tcggacacgc gtactttggg gacggctgtt ccgtttttct
    41101 caaatgcatc gatatagcgt tttttcgttt cccttaaagc cgcgctgctt aaagcacgcg
    41161 ttgcttcttc caaagtgatt tccttaaaaa ccggacggac gggcgaagtt ttgctttcag
    41221 actgcatcaa cccttcagca atactccggt cgaatgggat gcggcggttg tcgattacgg
    41281 cagttgcaat gtcttccgta tcggcagcct cttcctccgc agcttctgct gcttccgttt
    41341 ccgcttcttc cgtcccgttt ccactgtctg aatacccgtc ggatgcggtc ggaacatccg
    41401 cacggtttgc agaagcaggc ggaacgggcc tgtccggttc ggattcgtgc gccagatagt
    41461 ttcggcagaa acggacgaca ccgacaaaca gccaatacac tgccgttttg acggcatgga
    41521 aaagcatcag ggaaaactcg ggaaaatcgg gcataccgtc tttgatttca ggcagctccg
    41581 cctgtttttc ccctttcttt tcctgccacg cagaaacctc gcgcatccac tcgcgttcgg
    41641 attgtgcgcg gacaaaaaac aggccggcaa gcgcaagcaa caaaataacg atcaaaacta
    41701 tccaaaacat atgccgtttc ccgcaagaca aacgattaac cgagtttatt ttaacgtgtt
    41761 tccagcccaa aaaaaacaac cggccggcaa ttctgccgaa accgattcgg ctttcggttt
    41821 gcccccgccg acagtataat agccgcccct tcccttaatc tttgcggcat taccatgatt
    41881 ttccaaacag tctggttttc agatgtggta ctgtcggttt cgtggattgt ccttatcctg
    41941 atactggcag cttccgcgcc gtcggcattc cgttcgcttg cccgctaccg ttccgccctt
    42001 cccctgtgta ccgtaatttt ttccgcagca tggtgtctga acgcctctgt cggcggcgga
    42061 cagcttgccc agatgaacta ccacctgctt gccgtcaatc tggttacact gatggtggat
    42121 acctccgccg ccctttggct tgccgcgctg ctgatgctgc cctactgcct gctgtttgcc
    42181 ggttctgccg gcgcatatcc gccgaacgct ttggtgctga tactgcccgc gcttgtcgtc
    42241 aaccgcctgt cgcgtatgct ggtcaaccgt ctgccgccca atattttcat ctttattttc
    42301 gtcaacggct ttctcgcttc cgccgccggc attctgctga ccgggctggt gctgatcggt
    42361 attttggatg ccgcaaacgc ttttccgtcc gaaatattgt ggacgaccgc cctgcccgtc
    42421 tttattctgc tggcgtgggc ggaagccttc ctcagcggca tttcaactgc cattttcgtg
    42481 gcactgaagc cccattggat caacactttc gacgacaacc gctatctgaa gtccgagcgc
    42541 ggcatatggc tgtagccttc agacggcata ggcagacaaa accatgcttt ccaacatcct
    42601 ccccctttcc atcggtgcaa tattcggcac aacggcgcgt tggctgctca acctcgccgt
    42661 ccccgcatcg ttgtctcccg ccacaggcaa cctgtttgcc aactggacgg gcgcgctgct
    42721 catcggcatc tttgccgaaa ccgtcagcca tccgcaatgg aagctgctgc tgattaccgg
    42781 ttttttcggc agcctgacca cgctttccgg attttcactg gaaaccgtaa ccctgctcca
    42841 atcgaaccgt cccgcttcgg cacttgccaa tattttccta cacacggcag gttcgctgct
    42901 gctgacttgg ctcgggctga agatagggac ggcagtcaaa taaccgcaaa aaattgcttc
    42961 ctttggtttt gacataatct ataaattggc ttcgcttgtt taaatactgc ctgtgccaat
    43021 tatagtggat taaatttaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
    43081 ggcaaggcga ggcaacgccg taccggttta aatttaatcc actataccgt tctcaaattc
    43141 aaattgggct tttaggtcta taacaaagaa aaagccttgg gaggataaac ctaccaaggc
    43201 tttttcttaa aaaacgctct atcactgtgc ctaaaaatgc aaaaaagtaa ccgtcataat
    43261 ccttttgcca aaatcaatat catcatccga tgaatgaacg gacggtatat aagaaagcgt
    43321 taataaactc ataaaccagt aagaaaaatc agtaacgaag atattcatta ttacccattc
    43381 ttaaaaatta tcacgttcgt ttgggtaatt atcataccct tttccctctt attactaaag
    43441 cacgaattac tttaaagtac gcacccagag acaagccgaa aaggcatatc cgatccgcca
    43501 aacaaaatgc ccgccgactt actccggcgg gcggttccaa catcctgtca atccttcatc
    43561 agccagtatt gcaaaccgat taatgtttta ccgtctttaa tttcatcgtt tgccaatgcc
    43621 tgacggactt catctttcga catcagcacg gtttccgtta tttcatcttc atcattagaa
    43681 agcgtactgc ccaagcgcac gccttccgcc tcgaacagat acattttttc attgcaaaaa
    43741 ccgaccgccg tataaaaact gtacagcagg cgcacgctgt cggcaacata aggcgtttcc
    43801 tccgccaatt ctcgcagcgc acacgctgcc atatcctcgc ccgccacatc cagcttgccc
    43861 gcaggaagtt ccaatgtcgc ctgatttgcc gcataacgcc actgccgcac caaaacgacc
    43921 ttttcgtcat ccgttaccgc caacacgcac gccgcgcccg gatgccggat gacgatgcgc
    43981 tgcccttcat tgccgttggg cagcctgacc ttatccctgc tgatactgac gaaaccgccc
    44041 tcgtaaatgg tttcgccgcc taattttact tccctcaaat ccacttcata tctcctttcc
    44101 gtttcctatt ctgccgttca atcttcacgc ttgccgcgct cgatttccac tccaacctcg
    44161 cgcacgcctt ccaaaatacc cggcttgacg attttcacgt gcacccacac cgcgccgaaa
    44221 tatcccaaaa ccaaatcggc aatatattcc gccaacgctt ccaaaagcag gaaatcctgt
    44281 tctttcagat gtcggcgcag cgtttcgcat acctcggcat aatgcaccgt attggcaata
    44341 tcgtcgtccg aacccgcttt ctcgggaacg ccgatgtcca aatcgacaat cagggtctgc
    44401 aaccgttcgc gttcccagcc gtacacgccg ataagcgtat ccgccttcat gccgtgcaaa
    44461 aagattttat ccataatccg ccccgttttt tgctaaaata accgcaccat tctaagtaaa
    44521 gtaccgcaaa tgttcaacat accggccgtc gccgtttcct atttaatcgg ttcactttct
    44581 ttcgccgtca tcgtttccaa gtattacggg atggacgacc cgcgcaccta cggatcgggc
    44641 aatcccggcg cgaccaatgt tttacgcagc ggcaaaaaaa aggcggccgc gctgacgctc
    44701 ttgggcgatg ccgccaaagg tttggttgcc gttttgcttg cacgcgtgct tcaagaaccg
    44761 ctcggtttat ccgacagcgc aatcgccgcc gtcgcactcg ccgcgctggt cgggcatatg
    44821 tggccggtgt ttttcggatt taagggcggc aaaggcgtgg caacggcatt gggcgtgctt
    44881 ctggcactct ctcctgcaac tgccttggtc tgcgcgttga tttggcttgt gatggcattc
    44941 ggcttcaaag tatcctccct tgccgcgctg gtcgccacaa ccgccgcccc ccttgccgca
    45001 ctgtttttta tgccgcatac ttcttggatt ttcgcaaccc tcgcaatcgc catattggtg
    45061 ttgctccgcc ataagagcaa catcctcaac ctgattaaag gcaaagaaag caaaatcggc
    45121 gaaaaacgct gattcggcat ttgttttcca ttacgtcatg ccgtctgaaa ccatgttccc
    45181 gcgttcagac ggcatttccg cacaaacggc tttcatacat accatgctca gttacagaca
    45241 cgcatttcac gccggcaacc acgccgatat gctcaaacac ttcaccttgt ttttagtgct
    45301 gcaatatttc aaccgcaaag acaagcccta ctggtacatc gacacgcacg gcggcgcggg
    45361 tttgtacaat ctcgaaggca gcgaggcgca gaaagtcggc gaataccggc aaggcatcgc
    45421 cctgctccga caggcgcaaa acctgcctgc cgaactctct gactttgccg cgcacataca
    45481 aaaaatccta ccctcgcccg aactttactg cggctctccg tggctggcgc aatcgctgat
    45541 ccgcatcggc gacaaattgc gcctgtttga gctgcacccc accgactttg tccatctgca
    45601 aaacaatatg ggcgaagcgg gattgggcaa acgcggacaa gtgttgcgcg aagacggtta
    45661 caaagggctg atttccctgc tgccgccccc gccgcgacgc gctgttattc tcatcgaccc
    45721 gccctatgag gaaaaacagg actaccggcg tgtaacggaa acgctgaaag ccgctttaaa
    45781 acggtttgag tccggctgtt atctcatttg gtatccctgc ctcagccgcg aagaaagccg
    45841 caagctgccg gaagaattga aaaaactcct gcccgacaac tacctttacg ccgaactgca
    45901 cgtccacgcg ccgaaaaccg acggtttcgg tatgcacggc agcggaatgt tcgtgattaa
    45961 tcccccttac cttttagccg aacaattggc ggcaaacctc cccgccctga cacggctgct
    46021 ggcgcaagac gaaggcgcgc gctacctgtt ggacaacaaa atccgctgac cccgatgccg
    46081 tctgaaaccc ttcagacggc atcttccgtc aaacaaacaa aaccgggcga tacacactcc
    46141 atatcgtccg gttctgcata aaaccagtat tcaaaaatca gaacacgctt ttcattacac
    46201 ccacggtacc aaccacaccg ttcagcagca tcagaccacc ggcagccgta cccatacctt
    46261 gacggattgc gtcatgctta ttggcacgtt tttcacgttc caaagccaac tcctcagaaa
    46321 catttttacg ttgacggcgg tgttgcgcca gttccgcacg gctaattcgc gcttcggcac
    46381 ggttacgacc agccttgatt acggccgaat cgtcgccgat agcatagttg gctacagaat
    46441 tttcagaagc gcaggcagca agcaaaacgg cacacacagc aacaacggaa atatttttca
    46501 agtttttcat acctgtccta ttatctcaaa ataaagaaaa aactaatcca actggagttt
    46561 tatcggataa aataaatatt gggtgaattt tcgtttgcct ttttgatggc gcgtgccgca
    46621 tccatcattt cttcccggcg ttctttacgg tattcggcac gctcggcacg ctcgcttatc
    46681 tgaatggcac gacttttcgc ctgtatttcc gctgcagatt cttgagttac aaaacggcgg
    46741 tctgcgtaag tacatgccgt caccaacatc gcagccaccg acaacaaaat cagtttcttc
    46801 attttcatct acctatattt aagtttattt gtaaaatatg ttatcactat atatatatat
    46861 aatgtcaata ttttttaaaa ataaatttgg catcttgtac ttctcgatcc gcctgccgcg
    46921 ttccgacttg ccccgcgccg ttttttccca ctacaatccc acgcgatatc cggcgaaacg
    46981 caccaaagga acgccccgca gcaatctgcc gcctttcaga cggcacaaac ctttccgaaa
    47041 cgagcaaacc atggacacac tgacccgatt cctccccgaa cacctgcaac aaaaccaact
    47101 gcccgaagca ctcggcggcg ttttgctgtc cgtcgtttcc gcctgtaccg aaatcaacgc
    47161 caaagtccgc ctcggcgcac ttgccgacgt attgggtatg gcgggcacgg gcaatattca
    47221 gggtgaagac cagaaaaaac tggatgttat cgccaacaac attatgattg acacactcaa
    47281 agccaaccct gccgttgccg gtttggcaag cgaggaagaa gacactttcg taagtgccgg
    47341 tgaaaacgga cgctatctcg tcctattcga ccctttagac ggatcgtcca atattgatgt
    47401 caacatttcc gtcggtacga ttttctccat tctcgccaaa cccgaaggcg cattggcaac
    47461 cgaatcattc cttcaaacgg gcagacagca gcttgccgca ggctatgtcc tctacgggcc
    47521 tcaaacccag ctcgtattca cattcggaca cggcgtgtac gtatttacac tcaatgccga
    47581 aaacgaattt gtgctgacca aagaaaaccc gaaagtaccc gaaagtacca aagaattcgc
    47641 catcaatatg tctaaccgcc gccactggct accccccgtt caacaatacg tcgacgagct
    47701 cttggcgggc gaaaccggta cgcgcggcaa aaactacaat atgcgctggg tagccagtat
    47761 ggttgccgaa atccaccgca tcctgatgcg cggcggcgtg ttcatgtatc tgcaagacaa
    47821 acgcgacccg tccaaacccg gcaaactgcg cctgatgtac gaagccaacc ctatggccct
    47881 gatactcgaa caggcgggcg catcggcaag caacgcatat caagccatgc tcgatataca
    47941 gcccgaaagc ctgcaccaac gcgtcgccgt cattatgggc agcagcgaag aagtggatta
    48001 cctcaaccgt ctgcattcaa aataggcaga gaccgtactg ataaagaccg gcggcagcgt
    48061 gaatccgata ctgcgccgat atttcaagca taccctccgg aaaaacagaa aactgccttt
    48121 ccgaacgata aaaataccgt ctgaaaaatt ttcagacggt attttaaaaa ttaaagctca
    48181 aatttatttg cccgccgctt tttgcaggat ggcgtacaac tcgtctttca gtttcaattt
    48241 ggcttttttc agctcatcga tggtttccgc accgctggta accggattgt tgaccagacc
    48301 ggtaatttta tcgtccagct cgttatgttc gtcgaacaga cgggcgaagc gggaattttc
    48361 ctgtttcaat ttggaaatca aatcacggta ttctgggaac atatcgcttt cctctcttgg
    48421 tttacggtta aaaagtcaaa ttgaatcttt acattaatta taacaaagat aatattttta
    48481 tccagtgttt tgttacacac ggcaacaccg tccgcagaaa accgccccac cgtccgataa
    48541 aatcgttgac aatcttccgc tttacgcata aattttcagc ccttcctact gcatatgaaa
    48601 ggcacgcccc agtttcccca atgtggattt tcagcgcgcg cagtagaggt gccgacccaa
    48661 attggcagac cctttgcttt tgttaatatc ctagaaaatc cagaaattcg cagtactcta
    48721 ccactcatcg ccaattggcc aacctttcca cggctttggg ttaatggtga gttaatcggt
    48781 ggtagtgata ttatcctaca aatgtaccaa tcaggtgagc taaaaccact cattgaagag
    48841 catagcccaa aggtctgatt acttaatatc ttaaaataaa tatcaaaaaa gactgctgat
    48901 gacattagcg gtcttttttt gatggtcaag attgatagtt tggattgaat tatttagcca
    48961 tattacatac aataatcagc atgctttgta tgaattatca atcaaactca ggcgaaggag
    49021 tgcttgtagc taaaacatat ttattgactg cattgataat gtctatggta atctccggat
    49081 gtcaagtcat ccatgccaat caaggtaagg ttaatactaa ttctgctgtc atcgcaggtg
    49141 cagacgctca cacgcctgaa catgtaacgg gactgaccga acaaaagcag gtgattgcaa
    49201 gtgattttat agtagcgtca gccaatccat tagcaacaca agctggctat gatatcttaa
    49261 agcaaggcgg tagcgctgca gatgcgatgg tggcggtgca gacgacacta agcttggtag
    49321 agccacagtc gtcaggcttg ggcggtggtg catttgtgtt gtattgggac aataccgcca
    49381 aaacattgac cacatttgat gggcgtgaga cggcaccgat gcgtgcgacg ccagaattat
    49441 ttttggataa agatggttaa ccattgaaat ttatggaagc ggtggtcgct cggtaggtac
    49501 gcctgctatc cctaaactga cggagacaat acatcagcga tacggtgtat tgccttgggg
    49561 aaaattattt gatacgccga tccacttggc aaaacaaggc tttgaggtgt cgccaaggct
    49621 tgccatctcg gttgagcaaa atcagcagca tttggcacgc tatccaaaaa cagccgctta
    49681 ttttttgccg aatggtgtgc cgctacaagc aggcagcttg ctgaaaaatt tagaatttgc
    49741 tgacagtgtt caggcgttag cagctcaagg tgcaaaagct ctgcatactg gtaaatatgc
    49801 ccaaaatatt gtttcagttg tccaaaatgc taaggataac cccggtcaat tatccttgca
    49861 agatttatct gattatcaag tggtggaacg cccgcctgtt tgtgtgactt atcgtattta
    49921 tgaagtatgc ggtatgggtg caccaagctc aggtgggatt gctatgggtc agattttggg
    49981 gattttaaat gaattttcac ccaatcgggt ggggtatgat gctgaaggtt tgcgtttgcg
    50041 gatcgtgatg tatatttggg cgaccctgat tttgtaccag tacccattcg ccagttgatt
    50101 tctaaagact acctaaaaca tcgcagccag ctgcttgagc agtcggataa ggcattgcct
    50161 agcgtatctg caggcgattt tattcatgag tgggcgtcct cacaagcgat tgagttacct
    50221 tctaccagtc atatatcaat tgtggataag gcgggcaatg tgctatcaat gacgacttcc
    50281 attgaaaatg cgtttggctc aactttgatg gcaaatggct acctgctgaa taatgagctg
    50341 acagacttta gttttgagcc aataaagcag ggcaaacagg tcgccaatcg tgtcgagcct
    50401 ggcaagagac cacgctcctc aatggcacca accatcgtat ttaaagcagg caagccttat
    50461 atggcaatcg gctctccagg tggtagccgt atcattggct atgtcgctaa gacgattgtg
    50521 gcacatagtg actggaatat ggatatccaa gatgccatca gtgcacccaa tttattaaat
    50581 cgctttggta gctatgaact agaaacgggt acaaccgccg tccagtggca acaaacatta
    50641 aatgatttgg gctataagac tgatgtgcgt gagctaaatt caggtgttca agccattatt
    50701 atcgagccaa gcaggctggt tggtggtgtc gatccacgcc gagaaggtcg agtgacgggt
    50761 gattagaggt gaagcttgta ttttgtgaat ttgaccgcat taagttaatt attaaaatag
    50821 ggggaaattg tgagtaatca aacaaaaata caagccatca agcaaacttc tgagcagatc
    50881 ttggcaattt gtgagacgcc aaacacagca ttacaagcca ttcatctgat tttaagacat
    50941 ggcggggcag gaagaattgt cttggcaagt ggtgtatcag cgggttatgg ctgataaaga
    51001 tgtggttggt gctggttatt tgattgattt tgcacaaact gccgaaaatt taccatttga
    51061 tgtattgcct ttgattagcc ttgtgctaaa taaaggtgat gagacgctaa aaaccggcat
    51121 gttaaataaa ttacctgata atgccaaaga aaatttacgc attatgggct atctgcctta
    51181 ggttttgaag tattttttta tacgaaaaac gctcatgata ccctgattac acaaaggagc
    51241 cgagaatatg ttttcaaaaa gcgttaccct cgcacaatat gaccccgatt tggcagcagc
    51301 cattgcccaa gaagaccgac gccagcaaga ccacgtcgag ctgattgcct ctgaaaacta
    51361 cgtcagctgc gccgtgatgg aggcacaagg ttcgcaattg accaacaaat acgccgaagg
    51421 ctatcccgca aaacgctact acggcggctg cgaatacgtc gatattgtcg aacaattggc
    51481 aatcgaccgc gtaaaagaac tgtttggcgc agcctatgcc aacgttcaac cgcactccgg
    51541 ttcccaagcc aaccaagccg tatatgcttc cgttttaaaa ccgggcgaca ccattttggg
    51601 tatgtctctg gcgcacggcg gacacctgac ccacggcgcg agcgttaata tttccggcaa
    51661 actctacaat gccgttactt atggcttgga tgaaaacgaa gtcctcgatt atgccgaagt
    51721 cgaacgcctc gcgctcgaac acaaacccaa aatgattgtg gcgggcgcgt ctgcctacgc
    51781 gttgcaaatc gactgggcaa aattccgcga aatcgccgat aaagtcggcg catacctctt
    51841 tgtcgacatg gcgcactatg ccggactggt tgccggcggc gaatatccta accccgtgcc
    51901 attctgcgac ttcgtaacca ctaccaccca caaaacccta cgcggccctc gcggcggtgt
    51961 gattttgtgc cgtgacaata cccacgaaaa agcgctgaac tcttccatct tcccaagcct
    52021 gcaaggcggt ccgttgatgc acgttatcgc cgccaaagcc gtagcgttta aagaagcgtt
    52081 gcaacccgag ttcaaacaat acgcaaaaca agtgaaaatc aatgctgccg tcatggcgga
    52141 agagttggtt aaacgcggtt tgcgcatcgt ttccggccgc accgaaagcc acgttttcct
    52201 cgtcgacctg caaccgatga aaatcaccgg caaagccgcc gaagccgctt tgggcaaagc
    52261 gcacatcacc gtcaacaaaa acgccatccc gaacgatccg gaaaaaccgt tcgttacctc
    52321 cggcatccgc atcggttccg ccgccatgac cactcgcggc tttaacgaaa ccgacgctcg
    52381 cgtattgtcg aacttggttg ccgatgtatt ggctaatccc gaagacgaag ccaacctcgc
    52441 caaagtccgc ggacaagtga ctgccttgtg cgacaaatac cccgtttacg gaaactaaac
    52501 gcccgatgtt tccatacaaa atgccgtctg aacattttcc agacggcatt ttttgttttc
    52561 ggtaacattg ctattttttc tcttccagca aaatgaccaa ctttttcaat actgcgattt
    52621 cactatcttt ctgcctgatc aattcatcct ttgcatccag cagcgacctc aatttttcat
    52681 tttcaaacac cagcatttca tcagagccga taatattatg atggttattg gtattgtcgc
    52741 cgatagagaa gaaaaacgat cggttctgct ccgccagcag ctcaaccaca tcgatattga
    52801 aaatctgggc aatctggcgc agcttgtcga gattgatacc gcttttcccg cgttctattt
    52861 tcgaataccc gttaaccgac atttccaatt tttccgccat ctcttcctgc gtccactgat
    52921 tgacttccct taacgtccga atcttgtcat gcacttccat cctgccctcc aaggttaatt
    52981 ttcaccactc aaacaaccgt aaccggtatc ttcagttgat ggtcggatat tttaaaaata
    53041 aataatataa cacaatagta ttttgataaa aatttaccta acgaggtaca gacatgtatt
    53101 cagaaaacaa gcctaaacgc accgtaaaaa tgaaaaaata ctggcagcct gc
//
LOCUS       DAXFHF010000005        48364 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXFHF010000005 DAXFHF010000000
VERSION     DAXFHF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 48364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48364
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.005"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..801)
                     /locus_tag="ACFIRM_000231"
     CDS             complement(<1..801)
                     /locus_tag="ACFIRM_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC3027960.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVL"
     gene            886..1758
                     /locus_tag="ACFIRM_000232"
     CDS             886..1758
                     /locus_tag="ACFIRM_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC3027961.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(1816..2970)
                     /locus_tag="ACFIRM_000233"
     CDS             complement(1816..2970)
                     /locus_tag="ACFIRM_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC3027962.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3015..3383)
                     /locus_tag="ACFIRM_000234"
     CDS             complement(3015..3383)
                     /locus_tag="ACFIRM_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3027963.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            3557..4000
                     /locus_tag="ACFIRM_000235"
     CDS             3557..4000
                     /locus_tag="ACFIRM_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC3027964.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            4010..4138
                     /locus_tag="ACFIRM_000236"
     CDS             4010..4138
                     /locus_tag="ACFIRM_000236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027965.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(4162..4782)
                     /locus_tag="ACFIRM_000237"
     CDS             complement(4162..4782)
                     /locus_tag="ACFIRM_000237"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3027966.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(4893..6092)
                     /locus_tag="ACFIRM_000238"
     CDS             complement(4893..6092)
                     /locus_tag="ACFIRM_000238"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3027967.1"
                     /translation="MSDQLILVLNCVSSSFKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNKKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            complement(6404..7566)
                     /gene="prpF"
                     /locus_tag="ACFIRM_000239"
                     /pseudo
     CDS             complement(6404..7566)
                     /gene="prpF"
                     /locus_tag="ACFIRM_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            complement(8116..10721)
                     /gene="acnD"
                     /locus_tag="ACFIRM_000240"
                     /pseudo
     CDS             complement(8116..10721)
                     /gene="acnD"
                     /locus_tag="ACFIRM_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            complement(10798..11586)
                     /locus_tag="ACFIRM_000241"
     CDS             complement(10798..11586)
                     /locus_tag="ACFIRM_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC3027968.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVALLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(11738..12886)
                     /gene="prpC"
                     /locus_tag="ACFIRM_000242"
     CDS             complement(11738..12886)
                     /gene="prpC"
                     /locus_tag="ACFIRM_000242"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC3027969.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRSTGWAAHVLEQRKDGKIIRPSANYAGPEDLAFVEIEER"
     gene            complement(12971..13846)
                     /gene="prpB"
                     /locus_tag="ACFIRM_000243"
     CDS             complement(12971..13846)
                     /gene="prpB"
                     /locus_tag="ACFIRM_000243"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC3027970.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            complement(14466..15776)
                     /locus_tag="ACFIRM_000244"
     CDS             complement(14466..15776)
                     /locus_tag="ACFIRM_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC3027971.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(15912..17090)
                     /gene="ftsZ"
                     /locus_tag="ACFIRM_000245"
     CDS             complement(15912..17090)
                     /gene="ftsZ"
                     /locus_tag="ACFIRM_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC3027972.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(17209..18453)
                     /gene="ftsA"
                     /locus_tag="ACFIRM_000246"
     CDS             complement(17209..18453)
                     /gene="ftsA"
                     /locus_tag="ACFIRM_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC3027973.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(18539..19267)
                     /locus_tag="ACFIRM_000247"
     CDS             complement(18539..19267)
                     /locus_tag="ACFIRM_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC3027974.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYM
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(19257..20171)
                     /locus_tag="ACFIRM_000248"
     CDS             complement(19257..20171)
                     /locus_tag="ACFIRM_000248"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC3027975.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(20283..21692)
                     /gene="murC"
                     /locus_tag="ACFIRM_000249"
     CDS             complement(20283..21692)
                     /gene="murC"
                     /locus_tag="ACFIRM_000249"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC3027976.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(21847..22914)
                     /gene="murG"
                     /locus_tag="ACFIRM_000250"
     CDS             complement(21847..22914)
                     /gene="murG"
                     /locus_tag="ACFIRM_000250"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC3027977.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(22918..24189)
                     /locus_tag="ACFIRM_000251"
     CDS             complement(22918..24189)
                     /locus_tag="ACFIRM_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC3027978.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRQKMRGYRVE"
     gene            complement(24368..25705)
                     /gene="murD"
                     /locus_tag="ACFIRM_000252"
     CDS             complement(24368..25705)
                     /gene="murD"
                     /locus_tag="ACFIRM_000252"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC3027979.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAEIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(25790..26170)
                     /locus_tag="ACFIRM_000253"
     CDS             complement(25790..26170)
                     /locus_tag="ACFIRM_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3027980.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQE
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(26295..27377)
                     /gene="mraY"
                     /locus_tag="ACFIRM_000254"
     CDS             complement(26295..27377)
                     /gene="mraY"
                     /locus_tag="ACFIRM_000254"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC3027981.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(27622..27798)
                     /locus_tag="ACFIRM_000255"
     CDS             complement(27622..27798)
                     /locus_tag="ACFIRM_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3027982.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(27801..29159)
                     /gene="murF"
                     /locus_tag="ACFIRM_000256"
     CDS             complement(27801..29159)
                     /gene="murF"
                     /locus_tag="ACFIRM_000256"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC3027983.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(29284..30930)
                     /locus_tag="ACFIRM_000257"
     CDS             complement(29284..30930)
                     /locus_tag="ACFIRM_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3027984.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(30953..32431)
                     /locus_tag="ACFIRM_000258"
     CDS             complement(30953..32431)
                     /locus_tag="ACFIRM_000258"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC3027985.1"
                     /translation="MFSKLSPLAETGIPTLSCANAAGHLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEETAIIGTVGNGFWGALEETAHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(32456..34201)
                     /locus_tag="ACFIRM_000259"
     CDS             complement(32456..34201)
                     /locus_tag="ACFIRM_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC3027986.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(34262..34525)
                     /gene="ftsL"
                     /locus_tag="ACFIRM_000260"
     CDS             complement(34262..34525)
                     /gene="ftsL"
                     /locus_tag="ACFIRM_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC3027987.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(34552..35508)
                     /gene="rsmH"
                     /locus_tag="ACFIRM_000261"
     CDS             complement(34552..35508)
                     /gene="rsmH"
                     /locus_tag="ACFIRM_000261"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC3027988.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(35505..35960)
                     /gene="mraZ"
                     /locus_tag="ACFIRM_000262"
     CDS             complement(35505..35960)
                     /gene="mraZ"
                     /locus_tag="ACFIRM_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC3027989.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            36239..37486
                     /locus_tag="ACFIRM_000263"
     CDS             36239..37486
                     /locus_tag="ACFIRM_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3027990.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            37582..37658
                     /locus_tag="ACFIRM_000264"
     tRNA            37582..37658
                     /locus_tag="ACFIRM_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37616..37618,aa:Met,seq:cat)
     gene            37666..37741
                     /locus_tag="ACFIRM_000265"
     tRNA            37666..37741
                     /locus_tag="ACFIRM_000265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37699..37701,aa:Ala,seq:ggc)
     gene            complement(37927..38748)
                     /locus_tag="ACFIRM_000266"
     CDS             complement(37927..38748)
                     /locus_tag="ACFIRM_000266"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC3027991.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMFWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(38781..39425)
                     /locus_tag="ACFIRM_000267"
     CDS             complement(38781..39425)
                     /locus_tag="ACFIRM_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3027992.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(39428..40291)
                     /gene="ftsN"
                     /locus_tag="ACFIRM_000268"
     CDS             complement(39428..40291)
                     /gene="ftsN"
                     /locus_tag="ACFIRM_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3027993.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSESDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(40397..41893)
                     /locus_tag="ACFIRM_000269"
     CDS             complement(40397..41893)
                     /locus_tag="ACFIRM_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC3027994.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(41907..42227)
                     /locus_tag="ACFIRM_000270"
     CDS             complement(41907..42227)
                     /locus_tag="ACFIRM_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC3027995.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLARLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            42715..44241
                     /gene="putP"
                     /locus_tag="ACFIRM_000271"
     CDS             42715..44241
                     /gene="putP"
                     /locus_tag="ACFIRM_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC3027996.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            44493..48098
                     /gene="putA"
                     /locus_tag="ACFIRM_000272"
     CDS             44493..48098
                     /gene="putA"
                     /locus_tag="ACFIRM_000272"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC3027997.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKIHTDISYLACACKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
BASE COUNT        11222 a        13767 c        12295 g        11080 t
ORIGIN      
        1 caaaacccgg tgtttcaagg cgggcaggcg gttgcggacg ctgttcaggc ggttggcatc
       61 gatgtctgcc gtaacgatgc cttcgccttc gggtaatacg tccaacacgt cgccccacgg
      121 atcgacaatc atactgtgtc cgaacgtgcg ccgtccgttt tcgtgcaaac cgccctgtgc
      181 cgccgccacg acgtaacatt ggttttcgac ggcacgcgcg cgcagcagca gctcccagtg
      241 cgccttgccc gtcgtatggg taaatgccgc cggcagcatc aatacgtcaa acggcagttg
      301 gcggcggaaa aattcgggaa agcggacatc gtaacaaata cctgccgcca ccggcatgcc
      361 ttctgccgac aagtgcggca cttcccgccc tgcgcggatg gtatcggctt cggcatagcg
      421 ttcgcccaaa ccggaaaaac cgaagaggtg cattttgtgg tacagccccg tccttacgcc
      481 gtcgcacccg tacaccaata aagtattcat gaccttgccc gcttcggggc tttgcagcgg
      541 cacggttccg ccgaacagca ccacgccgca ttctttcgcc gtttcgctca atgccgtctg
      601 aaagcgtccg ccgcccaaag gctcggcaag tgcgagtttg ccggtatcgt ttgcgcccat
      661 cagcacccaa tattcgggca gcagcaccca atccgcaccc tgctccgccg cccgtgcgac
      721 caggcgtttc atggcggcga cgttggtttc cggcgacacg ccagacacca tctgcacggc
      781 ggcaactctg attttgtcca tttcttctcc tttttcctgc cgaccttgcg gcatattccg
      841 ccgataggct accatggcgg cactttgcga aaaccgttta cacaaatgcc cattttgaac
      901 catattgccc tcatcggtgt agggctgatc ggcggttcgt tcgttctcga cctcaaaagg
      961 cagggactcg tccgcaccgt taccggtatc gacaccgacc gcgacaacct cgaacgtgca
     1021 ttggaacgcg gcgtgattga ccgtgcgtcc gtcgtcatcg acgcggacag catcggcggt
     1081 gcggacttgg tactgattgc cacgcccgtc gccaccgttc ccgccgtttt gaccgcgctg
     1141 cgccccgttt tgccggaaca cacttggatt tccgatgtcg gcagcaccaa atcttcggtc
     1201 atcgaagcct tccgccgctg cctgcccgac cgcctgcacc gctgcatcgc cgcacacccg
     1261 attgccggtt cggacagaag cggtgcgcaa gccgcgcagt tcgggctgtt ccgccacaga
     1321 aaactcatca tcacgcccca cggcggcgaa gattcagacg gcatcgcatt ggtagaaaac
     1381 ctgtggcgcg cggtcggtgc ggacattttc acgatggacg cgcaacacca cgacgcggtt
     1441 ttcgccgccg tctcccatat gccccacctg accgccttcg cctatgtcca ccagattctc
     1501 gaccaccccg acgggcagga atatctgaaa ttcgccgcca cgggctttcg ggacttcacc
     1561 cgtatcgcct ccggccatcc cgccgtgtgg gcggacatct gccttgccaa caaagacagc
     1621 ctgctgcaac tggttcaagg cttgggcaaa cagttggacg ttttggcaga catcctgacc
     1681 gccgacgacc gcgaagccct gtaccgctat tttgaagaag ccaaaaccac gcgcgaccgc
     1741 tggctggacg gcaactgacc gccatgccgt ctgaaaagca aaccgccgca gacccgaaat
     1801 ctgcggcggt tttactcaac cgggcctgcc gtccgaacgc ggacggatca gccacggaat
     1861 gtatttccac gcatacacca gcagcgcgag tgcaaacaaa accgaagacg tgcggatgct
     1921 gtgcgtgtag gcagtgccgg aagaaaatac ggcaaccata cggacggcgg ttgccgccat
     1981 catcagccaa aacgcaacgg gaacggcttt gggcggcgga taaatcgaat tgcccgtatg
     2041 accgagcgcg gtacgcgcca tcatgcccaa agtcagcacg ccgataccgc cgaccccgat
     2101 cagatgtacg cccagattga ggaaggcagg tttgaaataa gacgcgccga ccgcaatcag
     2161 ccccaatccg gtaaacagat agccggcaaa cagaatccac agcatcggtt ctttcaatac
     2221 gggtttatac caccagcggt acacctgtac ggtaaaaatc acgcccgccg caaacgcgaa
     2281 agccgccgac agccaaggca tcacgccgtg cgccatcagt atggcggtca gcatgggtag
     2341 ccacagcgaa gcctgcgcca cccatttcgg actgggaatc tgcggcacgt tcaaccgttt
     2401 ggacgtaaaa aacgaaataa tcctcatccc aatcaggccg ataaagcccg acaccataac
     2461 caggcccgac tgcaatccgc tcaagagtcc gcctaggttg ccgttgtgca gctggacgtg
     2521 gaacgccgca tgcgtaccgc ccagcacaaa tattgcgaat acggcgacat agttgcgccg
     2581 gttttgcgaa cggataacgg gcaaagccat gcacaccgcg ccgtaccaga aaaacagcgt
     2641 accgagtatg ccgcttgccg ccgcacccca acccgggata aaggcggcaa tccgcgcagc
     2701 cagccaaaag gcggtcaagc cgaccagaac gccgcccctc gtgggcggct gtcccgtcca
     2761 agtggcgacg gcggtcagca ggaaggcgat gacgacgaga ccggcataac cccaaatcat
     2821 ctcatgcgcg tgccaataga aaccggacag ctcgtgcgtt cccgtgtagc cgaaacccca
     2881 cagcaatacg gacaatgcgc cgtacagtgc cgccagtgaa taaaacgggc ggaacgccat
     2941 tgcccagacg ggatgtttgg taaatttcat gtgtgttcct tgtttatcat taatatattt
     3001 gccgcaaaac ggaatcatgc cggcaaaaac ggttcgacgg cgggaaacag ctcgcgttct
     3061 tcaaagcgcg cgtggtcgcg cagggttgtg gcaaaagcgg tattccacgc cgcgttaccg
     3121 cattcggggc ttgccatcat ctgccgcagt ttggcgtgat cgccttcaaa acgggttttc
     3181 aactcaggcg cgacattctg ccaaattggg gcaaacttgg cttcttcttc gcgaaaatgg
     3241 gtttccagtt cggcaaaatg caactcgagt tcggcacgat ggtctgcccc gggcgtccgc
     3301 aacatacgca cgcacaggga aagcgagtgg tggtgttcgc gcgaaagccc gacaagggcg
     3361 gaatgtcgtt tcaacggttt cataaattta ggttacaata acaaattatc aaacggtagc
     3421 gcggatgttt cagacggcat accgccgcag acaggctaca ttctaccgcc ccgacggaca
     3481 gggtttcaac cattaaggta ttttccgtga aacttaaacc gtaccgataa gaataacgat
     3541 ccataccgag aaaatcatgt atctgaccca acataccgac tacgggctgc gcgtcctcat
     3601 ctacaccgcc gtcaacgacg acgcgctggt caatatcgcc accatcgcct cgacctacgg
     3661 catttccaaa agccatttga tgaaagtggt taccgcgctg gtcaaaggcg ggttcttgca
     3721 cagcgtacgc gggaaaggcg gcgggctgag gctggccgcg ccgcccgaac gcatcaatat
     3781 cggcgcggtc gtccgccacc tcgaaccgat gcagctggtc gagtgtatgg gcccgaacaa
     3841 cgaatgcctg attacgccgt cctgccggct gacgggcata ctcggcggtg caatgaagtc
     3901 gtttttcacg tatctggacg gtttcacgct gcaagacctg ctcaacaagc cgacctacga
     3961 cctgctttat gaatcgaaaa tcccgattgc ggtgcgataa cgcatcaaaa tgccgcccaa
     4021 acccgcgttc ggacggcatt ttgtttgccg tttcaaacgg atattccgac cgggcgcgat
     4081 acgcgccggc atttcgggct gccgccggca aggcgcgcga tgccgtctga acggctgatg
     4141 cttcagacgg catcggtttc cctacaaacc gaacaccgcc gtatcgccga aaccccggcg
     4201 caccagttcc gtgccgtcgg tcatgtcgat gacggtagtc ggatcggttc cgcaccagcc
     4261 gccgtcaatt accaaatcga cggcgtgttc caaacgctcg cggatttcat aagggtcggt
     4321 caatggttcg ccgtcttcgg gcagcatcag ggtgcagctt aaaagcggcc cgcccaactc
     4381 ctccagcagg gcttgtgcaa cggcattatc gggaatacgc agcccgatgg ttttgcgttt
     4441 cgggtgcagg gcgcgcgccg gcgcatcctt cgtcgcctgc aagataaagg tataaggccc
     4501 gggcgtggcg gctttaagct gacgaaactg tacgttgtcg attttggcgt atgtgcccaa
     4561 ctcgctcaaa tcggcgcaca tcagggtcag gtggtgtttc aaatcgattt ttcggatgga
     4621 gagtatgcgt tccatcgccg ccttatcgcc gagtttgcaa cccaaggcgt aacaggaatc
     4681 ggtcggataa accaccacgc cgccttgatt gatgatttcg actgcctgct tgatgaggcg
     4741 ttcttgggga ttgtcgggat gaacggcgaa aaattgtgcc atagtttgtt tccttatcgt
     4801 catgttttcc gtattgtacg cccaaacgcc aaacaaaatg ccgtctgaaa acctttcaga
     4861 cggcattttt gcggactcgc cccctttttt ggctacaaga tgccggcaag ttcggcagtg
     4921 tcgcaggcaa tcatcagttc ttcattggtc gggacgacca aaacagccgg agaagaatcg
     4981 gtcgggctga taatgcccga attgccgtag cgtttttcca tattggcttt ggtgtcgatg
     5041 tgcagaccca agaaatcaag ataggaaacg gttttggcac ggatattacg cgagttttcg
     5101 ccgataccgc cggtgaacac gagtgcgtca acaccgccgc aggccacagc catcgaagcg
     5161 atgtatttgg cgaggcggta ggtcatgact tcgagggcga ggcgcgcgcc ttcgcggcct
     5221 tcgtcggcgg cgatttcgag ggtgcggcag tcgttgggaa gttcggaaat accggggaaa
     5281 cctgattttt tgttcagcat ttcatcaact tgggcaacat ccatccctgc gtggaaagtc
     5341 ggatagctgt atacgcccgg atcggtgtcg ccgcaacgtg tacccattac caaaccttcg
     5401 atcggcgtga aacccatacc ggtatcgacg gatttgccgt ttttgacggc ggtaatggat
     5461 gcgccgttgc ctaagtgggc aataatcatg cggatgtctt ccagaggttt gcccaagatg
     5521 cgtgcggctt caggggcgac gtaacgcata ccggtaccgt ggaaaccgta gcggcggaag
     5581 gcgtattttt tgcgcaattc gcgcggcacg gcataagtgt aggcccgctc cggcatggtt
     5641 tggtggaacg aggtgtccat cacgccgacg ttgggcaggc cgggaaagtg ttcctgcgcg
     5701 gcgaggatgc cgctgatgtt ggcggggttg tgcagcgggg cgaacgggat gcaggctttc
     5761 agttcgtcaa ggacgtcttg gtcgatgagg acggactcgt gatatttttc gccgccgtgg
     5821 gcgatgcggt ggccgatggc tttgatgcgg tcgtgcagtc cgtgtttttc cagttcgttc
     5881 aacagcatac ccaccgcgcc ggcgtggcag ttgcggccgc tcaggggaac ttggcgtttg
     5941 ttgccgtctt tgttgaaggt aatgacggct tcgggcgtag tcaggcgttc cccgaggcag
     6001 cttaggacga cgctgccgct tttgcggtcg ataacggcgc ctttgaacga tgaactgacg
     6061 cagttcagga caagaatgag ttggtcggac atgatgtttc tccttgtagt ttgtggttgt
     6121 ttttttggtg cggggccgtc tgaaaaagga tgggcgttgg gcggagtgtg tttcagacgg
     6181 cctctttggg caaagcgtat gccgtatttc ctagcgtcat tcccacgcag gcgggaatcc
     6241 atatttgctc acacgcaggc tctgataaaa atcaggaaac agaatatcgg ctgcggattc
     6301 ccgcctgcgt gggaatgacg gcggtaaggt tattcggctt tatggtaatc cttaatcaaa
     6361 tgccgtctga aacagcggac agcaatgtgc gtcctacatg atttcaaaaa caatcatcgg
     6421 gaacgcgcac ccaaccttcc ataatcacgc gtgcgctgcg gctcatgacc gctttggcgg
     6481 ccgtccattg tccgtcctga cattcggcgg cagcaccgac acgcagcgta cctgacggat
     6541 gcccgaagcg cacttcttta cgcgttccgc cgcctgcggc aaggttgacc agcgtaccga
     6601 gcacggcggc ggcgatggcg accgaggcga tgcccatcat agcgtggtgc agtttgccca
     6661 tgctcagggc gcgtaccggc aaatcgatgt cggcggcgtt tacggttttg ccgctggagg
     6721 cggtgtaatc ggcggcgggc gcgacgaagg cgggtttcgg cgtgtgcgcg cgggcggcgg
     6781 cttcggatac gtcgctgatc aaacccattt tcagcgcgcc atatgcgcgg atggtttcaa
     6841 atttttccag cgcggcggca tcgttgttga tgtcgtcctg caactctttg cccgtgtagc
     6901 ccaagtcggc ggcgttcagg aaaacggtcg gaatgcccgc gttgatgagc gtggctttca
     6961 gacgacctat attcggcaca tcaatttcgt cgaccaaatt gccggttggg aacatactgc
     7021 cttcgccgtc ggctggatct aaaaattcga tttgtacttc ggctgccgga aaattacgcc
     7081 gtcaagctcg aaatcgcctg tttccaacac tgctccgttt tgcatcggca catgggcaat
     7141 aatggttttg ccgatgtttt tctgccagat tttgaccgtg cagatgccgt ctgaagggat
     7201 tttggattta tcgaccaagc cctgttcgat ggcgaatgcg cccacggcgg cggtaaggtt
     7261 gccgcagttg ccgctccaat cgacaaatgg tttgtcgatg gaaacttgtc cgaacaagta
     7321 atcgacatcg tgatcggcgc gttcggattt gtccgaaatc acagctttgc tggtggacga
     7381 actggcgttg cccaaaccgt caatctgttt gccgtaaggg tcggggctgc cgagtacgcg
     7441 caagaggatt ttgtcgcgcg ccccgcctgc ttcctgcgca gctgccggca gatcggtgcg
     7501 tttgaagaaa atgcctttag atgtaccgcc gcggtagtaa acggcgggga ttttgatttg
     7561 cggcatttta gggttctcct tatgtagcgt gggctctgcc cacgattttt atagtggatt
     7621 aatttaaacc ggtacgggtt gccccgcccc ggctcaaagg gaacgattcc ctaaggcgcc
     7681 caagcaccgg gcgaaccggt tccgtactat ccgtactgtc tgcggcccgc cgccttgtcc
     7741 tgatttttgt taatccgcta taccatgtca attcagcctt tatttcgtgg gcaaagccca
     7801 cgctacaccc actttccaga agtacattag gctaccactc cgaccaaagc cgtttgattc
     7861 cgcaaactgt cggggttcgc tccaatctac ggctgctgtg ttgtgatacg gtagattatt
     7921 aaaccccgtc attcccgcgt aggcggaaat ccagatttat ccgcacagaa actcatcgga
     7981 taaaaaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat gacgatttac
     8041 agtaaatgcc tgccatatcg ggaattacgt gaatgagaac ccgacccacg ctacgtcttg
     8101 cttttcagac gaactctaag ccgcgttccc ttccaaaaag tcctgtgcaa accgttgcaa
     8161 cacgccgccg gcttcatata ccaatacttc ttctgcagta tcgaggcgac aggtaaccgg
     8221 aacttcgacg gtttctccgt ttttacggtg aatcacgagg gtcaggccgc agcgcggtgt
     8281 gcgttcgccg acaacgtcgt aggtttccgt accgtccagt tgcagggtat ggcggttggt
     8341 gccgggtttg aattgcagcg gcaagacgcc catgccgatg aggttggtgc ggtggatgcg
     8401 ctcgaaacct tcggcggcga tggcttccac acccgccagc cgcacgccct tcgccgccca
     8461 gtcgcggctt gaaccttgac catagtccgc accggcaatg atgataagcg gctgtttgcg
     8521 gttcatatag gtttcgatgg cttcccacat gcgcatggtt tggccttctg gttcaacgcg
     8581 tgccaacgaa ccttggcgta cgctgccgtc ttcgtttctc accatttcgt taaacagttt
     8641 cggattggcg aaggttgcgc gttgggcggt caagtggtcg ccgcggtggg ttgcgtaaga
     8701 gttgaagtct tcttcaggca aacccatttt cgccaaatat tcacctgcgg cactgccggc
     8761 caaaatcgca ttggatggcg agatgtggtc ggtggtgatg ttgtcgggca aaatcgccag
     8821 cggacgcata cctcttaatg tacgttcccc tgccagtgcg ccttcccaat agggcggacg
     8881 gcggatgtag gtggacatcg gtcgccagtc gtacagcggg cttggtgctt tttgcgctgt
     8941 gccggtgtcg gacatcggga tataaatgtc gcggaattgt tgcggtttca catattcggc
     9001 aacgatggca tcgatttctt cgtctgtcgg ccagatatct ttcaggcgga tttcgcggcc
     9061 gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg atgctacctg ccaatgcgta
     9121 ggcaacgacc aaaggaggcg aagcgaggaa agcctgtttc gcatacggat ggatacggcc
     9181 gtcgaagttg cggttgcctg acaatacggc ggtggcgtac aaatcgcggt cgatgatttc
     9241 ttgttggatt ttcgggtcga gcgcgccgct catgccgtta caggtggtac atgcgaaggc
     9301 gacgataccg aagccgagtt tttccatttc gggcaacaag cctgcttctt tcaaatagat
     9361 tccggctact tttgaacccg gggcaaacga agatttcacc caaggtttgc gtttcaagcc
     9421 gaggcggttg gcattgcgtg ccaacagtgc ggcggcgaca acgttgcgcg ggttggaagt
     9481 attggtacac gaagtaatcg cggcaataat cactgcaccg tcaggcattt ggccgtctga
     9541 aggctcttcg taaggcttcg ccagcccttt cgccgccaaa tcggcggtgg caaaacgcgc
     9601 gtgcgggttg ctcgggcctg ccatattgcg cgttacgctg ctcaaatcaa atttcaaaac
     9661 gcgcggataa acggcggttt tcaagccacc tgcccataag cctgcggttt tggcgtaggt
     9721 ttccaccaat ttcacctgcg cgtcgtcacg tccggtcagt ttcaaataat caatagtttg
     9781 cgcgtcgatg gcgaacatgg cggcagtcgc gccgaactcc ggcgtcatgt tggaaatggt
     9841 cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg aaaaattcga caaacgcccc
     9901 gaccacgcgc tctttacgca agaattcggt cagtgccaac acgatgtctg tggcagtaat
     9961 gcccgcctgc cgtttgcccg tcagctccac gccgacaata tcgggcaggc gcatcatgga
    10021 cgcgcgtccc agcatcacgg tttccgcttc caaaccgccc acgccacgga aatcacgccc
    10081 agcgcatcaa cgtgcggcgt atgcgagtcc gtgccgacgc aggtatcggg gaaagccacg
    10141 ccgtttttga cttggacgac gggcgacatt ttttccagat tgatttggtg catgatgccg
    10201 ttgcccgccg gaatcacgtc cacattttca aatgcggttt ttgtccagtt gatgaagtgg
    10261 aaacggtctt cgttacgtct gtcttcgatt tcgcggtttt tgcggaaggc atcaggatcg
    10321 taaccgccgc actccaccgc caaagagtgg tcgacgataa gctgcgtctg caccaccgga
    10381 ttcactttgg caggatcgcc gcctttttcg gcaatcgcat cgcgcaagcc tgccaaatcc
    10441 accagcgcgg tctgccccag aatatcgtgg cacaccaccc gcgcgggata ccacggaaaa
    10501 tcgatttcct gcttcccttc tatcaactgg cccagccagc tttgcagcat cggcaaatcg
    10561 actttgtccg cgcggttgac caagttctca gacaaaatgc ggctcgtgta cggcagcttg
    10621 tcgtaagagc cgggtttgat gtcctcacac gccgcacgcg cgtcgtagta ttccaaatcc
    10681 gtaccgggca gcggtttgcg gtaactttgg ttggcagtca tgtcggttct cctgtggatc
    10741 tatttttctt gtggtttggg tttcagccga tgttttgaag gagtcgtctg aaagggttta
    10801 aaacatcgaa acaatcatct gatacagcgg atttctctcg tctatcaaca atttcacagc
    10861 catcgaaatg ctgatgacaa tcagcagcgg cttaatcagc ttcgaaccga agcggacggc
    10921 aaatctcgca cctaaattcg caccgacaaa cgcaccgacc gccatcgttg ccacaatcgg
    10981 gaaaataatc gaaccgtgca gcaggaatac cgatagcgaa ccaagattgc aagcaacgtt
    11041 cgccaatttg gtgtaagaca tcgcgttcaa cagcttgcag ccgagcaaaa caataaaggc
    11101 aatcagaaaa aacgagccga cacccggtcc gaacacaccg tcgtaaaaac ccaaaagcag
    11161 tgcaaccgtc agcccgaata gaaaaaaaga cattctggct ttgccttcct tactgccgtc
    11221 gagcttgggc gaaaacacaa aatacagcgc gacaaatatc aacaaaaccg gcacgaccgc
    11281 cagcaaaata tctttggaaa ccaagctgac cgataatgca ccgaccacgc cgcctgcaaa
    11341 cgatgctgcg gcaatcggga gacctttctt ccaatcaatc aaacctttgc gtgcaaaaga
    11401 aaccgtagcc gaaaacgtag cagcggctgc ttgcagcttg ttggtggcaa ttgccgacac
    11461 gggaggaata cctgccaaca agagtgcagg cagcgtaatc aaaccacccc cgcccgcaat
    11521 cgcatcgata aatccggcga tcatcgcaac caaacccaaa gcgagtatta tgtataaatc
    11581 ttccatgttt cttatcctgt tacttgcgcc aatacaggat tatcttctcc tattagatta
    11641 aacttatttc agacaacctt tccaataagg caaggtcgtc tgaaatcctc agctttgcat
    11701 accgaaataa agaacaaact gctattgcat tcttcaatta tcgttcttca atctccacaa
    11761 acgccaaatc ttcagggccg gcgtagtttg cgctcggacg gatgattttg ccgtctttgc
    11821 gctgttcgag gacgtgtgcc gcccagccgg tggatcgcga aatcacgaac agcggtgtga
    11881 acatagcggt cggtacgccc aatttttggt aggaaacggc agagaaccag tccagattcg
    11941 ggaacatttt tttctcttcc cacatcacgc tctccaagcg ttcggcgatg tcgaagaggc
    12001 gcatgtcgcc cgcttcccgg ctcaaaccgc gcgccacttc tttaatcacg acgttgcgcg
    12061 ggtcggaaat ggtgtaaacc ggatggccga aaccgatcac gatttctttg cggccgatgc
    12121 gttcgcggat gtcggcttcg tcggcgttgc ggtagcgttt ttggatgtcg taggcgactt
    12181 cgttcgcacc gccgtgtttc ggacctttaa gcgcgccgat ggcgccggtg atgcaggaat
    12241 acatatccga acccgtaccg gcaatcacgc gggcagtaaa ggtggaggcg ttgaactcgt
    12301 gttcggcata cagaatcagt gaaacatgca tggctttgat gtgtgattcg cttgggcgtt
    12361 tgccgtgcaa caggtgcagg aaatgaccgc cgacggtctc ttcgtcgctt tcaacttcaa
    12421 tacgtttgcc gttgtgcgaa tattgatacc agtacagcag gatgctgccg aggctggcga
    12481 tcagtttgtc ggcgatgtcg cgcgcttcac tttccggatg gctttcgcgt tcgggatgaa
    12541 cgcagcccag catggatacg ccggtacgca tcacgtccat cggatgggta tgtgcaggca
    12601 ggctttccaa aactttaatc acacggatag gcaggccgcg catggatttg agcttggttt
    12661 tataagcggc cagctcgaat ttgttgggca gatggccgtg aatcagcaag tgtgcgactt
    12721 cttcaaactc gcatttttgt gccaaattta gaatgtcgta accgcgatgg ctcaaatcgt
    12781 taccggtacg gccaacggta cacaaagcgg tattaccggc cgcaacacct gaaagcgcaa
    12841 cggatttttt aggtttgaag gtcggggttt gagtagtttc agtcatggta tttctccttt
    12901 gtatttttat gggttttatt tttcagacgg tcgatgcgga tttgttgaaa ggccgtctga
    12961 aagcggtaaa tcatttttga aacaatttat ccagtttttg atcgaaggca tgatagttca
    13021 gatgctcgta cagctcggca cgggtttgca tactgtccac caccgccgcc tgagtgccat
    13081 cgcgcataat cgcttcgtaa acattcaaag cggctttgct tgctgcgcgg aatgatgaca
    13141 acggatacag caccagtgac acgccgtttt cagccaactc gctttgcgta taaagcggtg
    13201 tagcaccaaa ctcggtaatg ttggccaata ctggtacttt cactgcatct gcaaattgac
    13261 ggtacatttt caaatcggtc atggcttcag ggaaaatcat gtccgcaccg gcttcgacac
    13321 aagcctgagc gcgttcgata gcggcatcca aaccttctac cgccagcgca tcggtacgcg
    13381 ccataatcac gaagttctca tcaacgcgcg catctacggc agctttgatg cggtcgacca
    13441 tttcatcttt ggaaacgatg gctttgttcg gacggtggcc gcagcgtttt tgcgctacct
    13501 gatcttcgat gtgaaccgct gcaacaccgg cgcgttcaaa gttgcgaatg gtacgggcaa
    13561 tattgaatgc accgccccaa cccacatcga tgtccaccag cagaggcgta tccacgttgt
    13621 ccgtaatgcg tcgtgcgtcg atcagcacat cttccattgt ggtaatgccc aaatcaggga
    13681 taccgcaaga acaggctgcc acgccgccgc cggacagata gatggctttg aaaccgcttt
    13741 gggtggccaa tcgtgcaaag taagcattga cgcaaccggc gacggcaagc ggattcgatt
    13801 ctttcacagc ttggcggaaa cgtgctccgg cagagtgttg actcatcata tttctccttt
    13861 atagactttt ttcagtatgg acaggcttcc atcacattcg gacggcaaaa cacagccatc
    13921 cgacgcgtcg ggcatcccaa tcattaaaaa tatatgggaa aaattatctt attgatattt
    13981 aaaacgaatc aaagaaaaac agcagaccgt tcggaattat gcggcaaaac cgcagacaag
    14041 aagaaaacaa ggggattatt cagaaaaggg aaaaacatct gaattggttt catagtaatg
    14101 ttcctttata gtattatgta gttttatttt tcggtaatct aaaacacaag actggaatgt
    14161 caaacatttt taaggagact aattacataa ttttattatt ttattttaac attatgatta
    14221 atataaatta attttaactt atttgtttcc gataaaatta ataggaaatt tagaaaaatt
    14281 ttcaataatc aaaatcctga aaaattataa aatattttat atataaaaca atgaattatt
    14341 ttcaagtaat ccgatgtagt ttattatcta tttgattttt atattattta tttaaaatta
    14401 aatatatctg gcaaacaaaa tgccgtctga aaacctttca gacggcattt ttaatatcga
    14461 atcaatcagc ccaaatacca gaatttcaat gcccacaata cggcaacaac ccataccata
    14521 ggcggcacgt ccccagtccg gcgacacaaa agtttgacca cggcatagct gatgaagccg
    14581 aaggcgatgc cgtctgcaat cgaataggta aacggcatga agacaatggt caggaacgcg
    14641 ggcgcggctt cagtcatatc gtcccaatca atgtccctcg cactgcggag catctgcgtg
    14701 ccgacataaa gcagtgcggg cgcggtggca aataccggaa cacttttcgc caatggggag
    14761 aacatcagac acgccagcat caatacgccg acggtaaccg ccgtcaggcc ggtccgtccg
    14821 cctgccgata cgcccgccgc gctttccaca taaggcgtgg ttgaagaagt acccaaagcc
    14881 gcacctgcca caatggcggt agagtctgca agcagtgcgc gtttcaggcg gggcagctta
    14941 ccgtccacca gcagtccggc acggtgggat acgccgacca gcgttccggt actgtcaaac
    15001 aaatcgacca agaagaagac gaaaatcacg ctgaccatgc tgacggtaaa cagaccttta
    15061 aaatccatct gcataaaggt cggcgcaatg cccggtactt cgccgaccac gccgtgaaac
    15121 tcgttcaaac ccatcaggct ggcaatgacg gtaatcgtca gaatggtgat gatgattgcg
    15181 ccttgaacgc ggaaataccc caatacgacc accatgacaa aaccgaacaa tgccaacagt
    15241 gcgctgggct gatgaatatc gcccaagccg accaaggttg ccggattggc aacgataatg
    15301 cctgcgcctt tcagggaaat cagtgccaaa aacaaaccga taccggcggc aatcgacatt
    15361 ttcaaaccca taggcagtgc gttgaccagc atttccctga ctttaaaaaa gctgaacagg
    15421 atgaaaatca gaccggaaat gaacaccgca cccaacgcca cctgccaagg cacgcccata
    15481 cccttaacga cggcaaaggt gaaataggca ttcagcccca tccccggggc aagcgcaatc
    15541 ggatagttgc cgataaaacc catgacaaaa cagccgatgg cggatgcgat acaggtggcg
    15601 acgaataccg cccccatatc cattccggtc tcgcccaaaa tcaggggatt gacgataacg
    15661 atgtagcaca tcgtcaaaaa ggtcgtcaaa cccgccatca actcggtacg taccgtcgta
    15721 ccgtttgcct tcaggttaaa aaccctatcc agcaatgttt gttttgaaat atccatatct
    15781 agcagtccgt ttcaagtaaa aaatctgaac ccgcacaccg caaaccgcac aggattcggc
    15841 atattgaaaa atatacctcc caaatcggga aaaccggttc gggaggcaat aagtttacgg
    15901 cagacggcac attatttgtc tgaattgtgt tgacgacgca aaattgcagg aatttcaaaa
    15961 tcgtcaagga cagactggtt atcgaaatcc gctgctgtca gattcatggt gcggataccg
    16021 cgattggtgc ggatcatacc ttcgacattg tggctttgct cctgtttgga cggggcaacc
    16081 gcttctactt cccttgccgg agtaggatcg accgctcctt tttctttcag accggtagcg
    16141 ataatggtaa tccggatggc atcttcgctc atggtctcgt cttcagcggc accgaatttg
    16201 cattccaaat cgggatgcgc gctttggttg acgattttca tgacttcaga caactcggac
    16261 attttcaggc aacccggagc agtcgtaata ttgaccagca caccgcgtgc accatccaaa
    16321 gttacatcgt ccagcagcgg actggaaatg gcctggtcgg tcgccatacg cgcacggtcg
    16381 ataccttggg cataacccga acccatcata gcgataccgc ggttgctcat cacggttttc
    16441 acgtcggcaa agtcgaggtt gatgatttcg ctcgggcaag ttaccacttc ggaaatgcct
    16501 gcgaccgcat cgcgcaatac attgtccgcc gcgcggaagg cttcgcgcat agttacgtct
    16561 tcacccaatg cggtcatcag tttgtcgttc gggatgataa tcagcgaatc gacgtgttct
    16621 ttcaactgtt ccaaccctgc ctgtgccaca tggacgcgtt tgccttcata agaaaacgga
    16681 cgggtaacta cggcaacggt cagaatgccc aaagacttgg caatctcagc aacaaccggc
    16741 gcggaaccgg taccggtacc gccgcccata ccggtcgtga taaacagcat attggcaccg
    16801 cgaatggctt cttcaatggc ctcccgatct tcctgtgctg ccgcacggcc gatatcggga
    16861 ttcgcacccg cgcccaaacc gcgtgtcaga ttcgtaccca actggattct cttcgccgca
    16921 tggttttttg ccaaagactg cgcatctgta ttggcactga taaactccac actgcgcaca
    16981 ttgttggcaa ccatgttatt gattgcattg caaccgccgc cgcccaagcc gattacttta
    17041 attaccgcag ggctgactgc cgattctgcc acgtcgtaaa caaattccat tcaaaaactc
    17101 ctgatcgccc cgttcagagg acggtttaaa taaattatta tttattatat aagaagtatc
    17161 ttactgctgg caaaatactt ctcacctgtc aaacggcaat ccaactgttc agaggttgtt
    17221 ttcaatccac cgcttcaatc ttgccaacaa accgccgccc ccttctctct cttgcactgt
    17281 accgttttcc ggttgcggca agtttccttc cagcttgcat gctgcatgaa gcagcccgat
    17341 agcggtagaa aagcgcggtg tgcggacacg gtcggacaaa ccgcccattt cttggggcgc
    17401 accggtgcgt acaggcaaat cgaagatttt ttcggcaaat tccacaatcc cggtcatcat
    17461 ggacacaccg ccggtcagaa cgatacccgc attcagcact tctttgggga aacccgattt
    17521 ttgcagttcg cccagcacta cgccaaaaat ctcctgaata cgtgcgctga tgattgctgc
    17581 caaaacctta ctggaaacct gacgcgatgt ccggtcaccc acgcccggaa cttcaatcat
    17641 ctcgcccaag ccttccgtat cgcatgatgc cacgccataa tggattttaa tgtactcggc
    17701 ggcatcgaga ggtgttctca acgatttgga caaatctttg gtaatcagat taccaccggc
    17761 cggaatgacg gacgtatggc ggatggcgcc gttcatataa acggcaatat cggtcgttcc
    17821 gccaccaatg tcgatgacgc atacgccgag gtctttttca tcttcagtca gcaccgcctg
    17881 cccgcttgcc aacggttgaa gcatgatctg atcgcttttc aagccgcaca gctcgataca
    17941 tttttggaca ttctgcactg ccgtacttgc accggtaatg atgtgcaccc gcgtatccag
    18001 acgcacaccg ctcataccga tgggctccct tacaccaagt tgggtgtcga taatgtagtc
    18061 ttgaaccacg gcatcgagaa ttttttgatc gggcgggata ttgattgcct ttgccgtttc
    18121 aatggcgcgg tcgatgtctg cctgtgttac ttctccgtct ttgattttaa ccacaccttg
    18181 cgaattaagg ctgcggatgt ggttgcctgc gatacctgtg gtaacgtggg taattttggt
    18241 atccgccatc agctcggcat cattgaccgc ctgcctgatg gcttggacgg tggcatcgat
    18301 attggttacc atgcccgcgc gcaagccccg tgaaggagcc tgccccaaac cgatgatgtt
    18361 gattttgtcg tcatcttgaa cttccccgat cagtgcgagg actttagacg taccgatatc
    18421 cagtacgctg atgtatcttt tctgctgttc cattgttcgt ctgcccttaa aactgattga
    18481 aatttgcgtc gcaccgtttc agacggcacg gccgtaatat gtccgatacc tgttcccact
    18541 attcttcgga ttctttttcg ggtaaaccgt cgggagcatg gcggactgaa aatccgtcct
    18601 tatacctcat atccatatag gataaccgat ttttattctt acgcaacaga tgctgccacg
    18661 cttcggtaaa aagccggagg cgtttcatct cgttttcccg tcccagcctg acggtgatgc
    18721 cgttgtccaa aacgacattc cacgccgaac gtgccgtata ggtcatctct ttgatgccca
    18781 aaccctgttt tgccaaaaca gtcgaaaatt cgtcataacg gcggagcatt tcggcagacg
    18841 ttccttccgc gcctctgaat accggcattc cgggtctgtc caagcgggct tcaaaaacat
    18901 tgccttcgcc gtccaccaag gcatggtcgc cccaacgtgc aaccggcttg cgctcggtca
    18961 ggacgacctc aaccgtatcg ggaaaacggc ggcgcaccat gaccgacgca atccacggat
    19021 accggcggta ggcttcctgt gcgccattga tgtccgtcct caaaatattc ccatggatgt
    19081 attctttcgc caaactgccc aatgccttct tatcggaata aaccaggttg cccttcagcg
    19141 acacctgctt gacgggcaga tgattcgaat tgtaaaacca aaccagcccg gacgcagcaa
    19201 gcagcatcgc catcatgaca agcagccagc gcgtcagccg ttccatagct tcggcattat
    19261 cccacatgtg cggccttcaa aatttcaata cataaatcgg caaaacccac gcccgtaacg
    19321 gcagcggatt tcggtactaa actatggccg gtcataccgg gcagggtgtt gatttccaac
    19381 agatagagtt tgccgtcggt atctttgagg aaatcgacgc gcacgcagcc ttccgcaccg
    19441 attgcctgtg cgccgcgaac cgccagttcg cgcatcaggc tttcttcggc ttcggtcaaa
    19501 tcttccgaag gacattgata aatggtgtcg tctcggttgt acttggcttc gtagtcgtaa
    19561 aactcggttg cggggatgat gtgtatgccg ggcagccctt tgccgttcag gacggggcag
    19621 gaatattcgc cgccgccgat aaaacgttcg gcaatgattt cgccctgaag gtgtttcaat
    19681 tcttcgtaaa cgcttttcag acggcctttt tctttgactt ttaccacgcc gacgctgctg
    19741 ccttcggccg ccggcttcac aaacatcggc agacccaatt tttcttcgac ggcatcgaaa
    19801 tcggtatcat cgtacagtac ggcgaactcg ggaacgggta atcccaatgc ctgccaaatc
    19861 agtttgcagc ggtatttgtc catgccgatg gcggaggcgg cgacaccgct gccggtatag
    19921 ggaatgccca acagttccaa tgcaccctga acagccccgt cttcgccgta agtaccgtga
    19981 aggatgttga atgccgtctg aaagccccgc tccttcagtt cggataacgg cgtttcctta
    20041 gggtcgaagg cgtatgcgtc tatgcctttg ctttttaagg cgttcaaaat ggcggtaccg
    20101 ctgtccagcg agatttctcg ttcgctggaa aaaccaccca tcaatacggc cactttgcca
    20161 aaattctgca ttgtttttgt tctttcctaa ttgctttatg cttgttgtca gaggtcgtct
    20221 gaaacctgat tgcggtttca gacgaccttt atatgatgtt ccgtctgtca ggcgggtgta
    20281 tctcaaatct gtttcgacaa ttccaacagc gcggagggaa cgcggttgat gcttcccgca
    20341 cccatattca acacaacatc gccatcctgt aaaacattca tcagcatttg cggcaggtcg
    20401 gcgacgtttt cgcagtaaat cggctcaagt ttgcccaata cgcggatagc acgcgccaag
    20461 gcgcgggagt cggcggcggc aaccggctct tcgccggcgg cataaacttc ggtcagtacc
    20521 agcgcatcaa cggtattgag tactttggta aagtcttcaa acaaatcgcg cgtgcgggta
    20581 tagcggtgcg gctggaaggc gagcaccaaa cgtttttccg gatacgcgcc gcgtgcagcg
    20641 gcaagggttg ccgccatttc gacggggtgg tgtccgtaat cgtccaccag caaagcggtc
    20701 ccgccgtttg gcaacttgat gtcgccgtat ttttggaagc ggcggccgac gccttcaaag
    20761 ccgagcaagc ctttttggat cgcttcaacc gatgcgccga cttccagcgc cacgccgatg
    20821 gctgccaatg cgttcagcac gttgtgtctg ccgggcatat tcagcacgac ttcaaacgac
    20881 ccctgctcat gtcctttcat ttgaacatgg acggtgaatt tcatttgcgc gccgacgttt
    20941 tcgatgtcgg tggcgtagat gtcggcggta tcgtccaaac cgtaagtagc ataaggtttg
    21001 ctcactttgg gcaaaatcgc gcggacgtgt tcgctgtcaa cacacaaaaa ggctttgccg
    21061 tagaagggca tacggtggat gaaatcgata aacgcctgat gcagtttttc gacgctgtgc
    21121 ccgtaggtat ccatatggtc ttcgtcgata ttggtaacga cggacataat cggggtcaga
    21181 tgtaggaaag aggcatcgga ttcgtcggct tcggcaacga tgtattcgcc tttgcccaag
    21241 cgggcgttgg tgcctgcggc gttgagtttg ccgccgataa cgaaagtggg gtcgagtcct
    21301 gccgcgccga ggatggaggc ggtcaggctg gtggtcgtgg ttttgccgtg cgtaccggca
    21361 atggcaatgc cgtcacggaa gcgcatcagc tctgccagca tcaaggcgcg cggaataacg
    21421 ggaatttgcc gctccaacgc agcgacaact tcgggatttt ctttcttgac ggcggtagag
    21481 gcaacgacga catccgcacc gttaacgtgt tctgcggtat ggccgggata aacttgaatg
    21541 cccaggctgc tcaaatgctc ggtagcggca tttcgcgcct gatccgaacc ggaaacttta
    21601 aagcccaaat tgtgcaagac ttcggcgata ccgctcatgc cgacgccgcc gataccgaca
    21661 aaatggatgt tgcttactcg atttttcatc ataatgttgc gttccggttg gtttttagtg
    21721 cgtaaaggcg ttattttaaa ggtctgaccg tttgcgcacc atagttttct gacaaatata
    21781 tagcggattg aaataaaaaa catccatgcc gtctgaacgg cttttcagac ggcatggttc
    21841 ggcagtttac gccgcacacg caatcgcggc ttcggcaacg tcatccgcgc tgtgcggcaa
    21901 tgccaacgta cgggcgtttt ccgcccattt gaggcatttt tcgcggttga ggctgccgag
    21961 gatttcggcg agtttttccg ccgtcaactg ggtttgcggc aacagcagcc ccgcttctgc
    22021 ctgcaccatg aaacgcgcgt tggcggtttg atggtcatca acggcgtgag gatacggcac
    22081 taacaacgcg cccagccccg ccgccgtcaa ctcggcaatc gtcagcgcgc cggcacggca
    22141 aatcaccaaa tcggcatcac ggtaggcgga caccatgtcg gtaataaatt ccacgcattc
    22201 cgctttcacg cccaacgcgt cataatccgc ctgaagattg cccagcttgt tacgccccga
    22261 ctggtggtac atctgcgggc gcacctcttc aggcagcagt gccaacgcct gcggtacggt
    22321 tttgttcaaa acgtccgcac ccaaactgcc gccgaccacc aaaattttca gacggccttc
    22381 gcgcccttgg aagcgttcgg caggcacggg caggttgcta atatcggcgc ggacggggtt
    22441 gccgaccaaa ccgccttcgt ggctgaacgc tttcggaaaa gcgtacaaca cccgtttcgc
    22501 ccagcgcgac aggtggcggt tggacaagcc tgccacggcg ttttgctcgt gaatcacaat
    22561 cggcacgccc aagagtttcg ccgccagacc gccgggaaag gtaacaaaac cgccgaagcc
    22621 gatgacgcac tcgacacggt gtttgcggat aatccgctgc gcttcgcgga cggttttgta
    22681 cagagtaaac ggaagcatca gcttgcgttt gatgccgttg ccgcgtattc ctttaatcgc
    22741 cagcgtttcc aagcgtatgc cgtattgcgg cacgatgcgc tcttccatcg aatccttgct
    22801 gcccagccaa attacatgat gaccgcgcac gcgcaatgaa tccgccacag ccagagctgg
    22861 gaaaatgtgt ccgcccgttc cgcccgccat cagcataaag gttttaccgc ccatgattta
    22921 ctccacccgg taaccgcgca ttttctggcg gttttcataa tcgatacgca acagcagcat
    22981 catgctgatc agcatgaaaa agactgacga accgccatag gacatcaacg gcagcgtcag
    23041 acctttggtc ggcaaagcac cgatgttcac accgatattg aagaaacttt ggataccgat
    23101 ccaaatgccg atacccgaag cgatataggc gttgaaagtc aaacccaaat cgcgcgactg
    23161 cttgccgatg gaaaacgccc gcaccaccag ccagccgtaa cagaatatca gcacgcacat
    23221 cccgaagaag ccgaattctt cagcgatgat ggcaaaaata aaatcggtat gcgcttccgg
    23281 cagaaagccg cgtttgctca aactcgcacc caaacccata ccgaaccact ctccgcgccc
    23341 gattgccatc agagagtggg taagctggta gccggcaccc tgcgggtctt tccacgggtc
    23401 caaaaatgcc actacccgct gcacacggta gggagcggcg gtaatcatca gcaccatccc
    23461 acccaagacg ctgcctacca ggacaaaaaa atatttccac ggcaatcctg ccagaaacag
    23521 cattccaacg gtaatgacgg taatgacgac aaacgaaccg aaatccggct gtaccattat
    23581 cagcaccaaa ccgaacgcca ccagcataat cggcaggatg atcgcccgga aacggccgta
    23641 catttctaat gtttcacgac gtgcctgcgg attggtggcg gacataatca ggttggccgt
    23701 cccccgccaa atcgactgcc aacccaaact ttccatgctg cgcaacactt cttcacggcg
    23761 cgtgaacagg cttgccaaat aaaggatgac tgccagcttg aacagctcgg tcggctggaa
    23821 attcaacgga cccaaaggta tccaacgggt cgcgccattg atttcgcgcc cggcaatcaa
    23881 tacggctacc agcaacaggc cggataaggc aaaaatccac ggcacaagcc gccgccatgt
    23941 cctcatcctg caaagaaacc ataaaaaacc gctcgctata aggccggcaa cgacgaaccc
    24001 cgcctgcctg gtcaaataga aaaactgatc gccgccttcc ttcgatgcca aatacacaga
    24061 agccgaataa atcatcagca ggccgaacgc cgtcatcagc accaccatcc acaaaagcgg
    24121 cgcgtcgaat ttcctgccgt cgcgcacaat cggcctgtcg agcagcagag tgtggacacc
    24181 gtcgcccact tttaccaata cttccgaaat cttcaaaaaa ccacctgcca gtctgtttgc
    24241 acctgccgca aagggcaaaa atttcagacg gcagacaatg ccgtccgaac atacgataca
    24301 tcccaaatcg gtattctaaa tctttacttg ccgcccaaca atgacggcgt ttgcgtttcg
    24361 gacggcatca caaagcctta aacgcttcga taaacacttc cgaacggtgc gcgtagcctt
    24421 taaacatatc gaaactcgcg caggcggggc tgagcaagac aatatcgccc gcttcggctt
    24481 gggcgtatgc cgtctgaacc gcctcttcca aagtgacgca gtcggtcagg ttcaagccgc
    24541 agccgtccaa atcgcggcgg atttgcggcg catcgacgcc gatcaggaac acgccttttg
    24601 ccttatcttt caacgcgtcg cgcaggggcg tgaagtcctg ccctttgccc atgccgccca
    24661 aaatcacgaa gagcgggttt tgcaaaccgg caatcgcggc ggcggtcgcg ccgacattcg
    24721 tgcctttgct gtcgtcgatg aataccacgc cgtttttctc gccgattttt tccacacggt
    24781 gcggcaagcc ttggaaggtt ttgacgtgtt ccagcaatgc ttcgcgcggc aaaccgacgg
    24841 cctcgcacaa agccacggca gccataacgt tggcggcgtt gtgcaaacct tgcagcggga
    24901 tgtcttgcgt agaaatcaaa tcttcattgc cttgttttaa acagcccgtc ccgcgttcca
    24961 accaaaaatc ggcttcgtgt tccaaggaaa accgtttcac ttcacgccct gcccgtttca
    25021 tggcgcggca gaacacgtcg tccgcattca aaacctgcac tccatcgcca cggaaaatct
    25081 cggctttggt atgcgcgtag tcgagcaaat cgtcgtagcg gtcgagatgg tcttcggaaa
    25141 tgttcatcac cgtcgccgca gtcgggcgca ggctttcggt gttttccagt tggaagctgg
    25201 aaagctccaa cacccacacg tccgcctttt tgccttcgcg ctgcaattct gcctccaaaa
    25261 ccggcgtacc gatattgccc gcaatgacgg tatccagccc gcacttgatg cagagatagc
    25321 cgaccaggct cgttaccgtg gttttgccgt tgctgccggt aatcgcaatc accttgtcgc
    25381 cgcggcggtt cacaatgtcc gccagcaatt cgatgtcgcc tagcacgcgc ccgccgtttt
    25441 gcttgaacgc ctcgatatcc ggctgccgct cgctgatgcc gggactgagc gccagaatat
    25501 cgaaaccgtt gtccaacgca tctttcagac gacccgtgta aaacaccagc ccgtcaaaca
    25561 tcttaccgat ttgcgccacg cgctcggctt tcagctccgc atcataagcg gcaacctccg
    25621 cgccgttttt gcgcagatag gcaatcatgg aaatacccgt gccgccaagt ccggcgacga
    25681 gggttttttt gttttggaaa gtcattttgg ttttgtccta aaacaaatca tattgggcag
    25741 gagacgtccg cccttgccca agccgctttc agacggcatc gcgagccgat taataacccg
    25801 ccttcaggcg ttggtcattg tcgcagctgt tttggtctcc gttttgacaa gccttgccaa
    25861 gccattcttg agcgagcgca aggtcttggc gcacgccgcg tccatcgtaa tacatcaagc
    25921 ccaaattgta ttgggcttgg gcatcccctt gttctgaagc cttgcgatac cactgcactg
    25981 cctgtacata atcttgacga actccttgtc cattttcata catcacgccc aaattgaatt
    26041 gggctgctgc atttccctgt tctgccgcct gcaagttttc ccgaaaatcc gatacgtcac
    26101 ccgcccacac cgcttggttc aagcccaagg caatcagggc ggcggcaagc catttgactg
    26161 tctgtttcat ggttttctca tgttttagta taaggcgggt ttcagccgcc gttaacgata
    26221 ggacggggcg gattgccgcc gcaggtttat tgcgcgttca aatgccgtct gaaaggtgtt
    26281 cggacggcat aggttcagcg aattttgagg gtactcaaac cgatcaatac caagacgatg
    26341 gtaataatcc agaaacggac gacgacttgg gtttctttcc agcctttttg ttcgtaatgg
    26401 tgatgaatcg gcgccatcag gaagatgcgt tttttggttt tcttgtacca gccgacctga
    26461 agcataacgg acacggcttc tacgacgaac agaccgccca taatgacgag gacaaactct
    26521 tggcggacga tgacggcaac ggtaccgagc gcggcaccca atgccagcgc gccgacatcg
    26581 cccataaaga cttgcgcggg ataggcgttg aaccacaaaa atccgaggca cgcgccgcac
    26641 atggcggtgc agaatatagc gacttcgttc gcgccggcga catagggaag ctggaggtat
    26701 tgggaaaatt ggtagtgtcc gctgacgtag gcgaaaatgg cgagcccggc ggcaacgagg
    26761 acgacgggga aggcggcaag gccgtccaag ccgtcggtga ggttgacggc gttggatgtg
    26821 ccgacgatgg tcaggtaaga caacaccaaa aagccgacca cgcccagcgg cagggcgatt
    26881 tgtttgaaaa acgggacaat caaaatattg ttggcggaat tggcggcaag gtaaaacaat
    26941 gccaaaccgg cgataacggc aacgcttgac tgccacacca ttttgaattt ggcggacacg
    27001 ccgttggggt ctttataaac gactttgcgc cagtcgtcgt aaaaaccgag cgcaccggtg
    27061 gcaagcagta cgcccaagag aatccagata tacgggttcg cccagttgcc ccacaacagg
    27121 gtggacacgg taatggcggt cagaatcagc gaaccgccca tcgtcggcgt gccgtttttg
    27181 acgaggtggg tttgcgggcc gtcggtacgc actgcctgcc cgcatttgag cgcggtcagc
    27241 ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcca aggcggtcaa cgccgccata
    27301 acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt gctgaaatgt
    27361 gcgagccata aaaacatggg gcttcctttt ttgttttgtc gttatagcgg attaacaaaa
    27421 accggtacgt cgttgccccg ccccggctca aagggaacgg ttccctaagg cgcccaagca
    27481 ccgggcggat cggttccgta ctatccgtac tgtctgcggc ccgccgcctt gtcctgattt
    27541 ttgttaatcc gctatacatc gggcttgcgc ccgcttgagg tttgcttgct gtccggcaag
    27601 gtttcggacg gcttttgcag attaacgtgc agggactttc ccaatcacgc aaatcgggta
    27661 gctccccgga tttttgccgc cgcccaagtc taaacataaa tcttcataaa gatacgcctg
    27721 cgtccgcatt ccggcataaa acgcgccgac cagcgcggca atcagcaata aggctttaaa
    27781 aaaacgtcgg cttttcattt tcacttatcc tccaatgcct cgaccacttc ttccatctgc
    27841 ataaagcgcg aacctttcac caacacggtg gcgcgttcgg gcaaatcgtg gctcaacact
    27901 tgaatcaacg ggtctttggc ggcgaaccac aaaccgtccg cgccaaattt ttccgccgct
    27961 tcgacgctgt tgtcgccgac aaaataagcc gcttcgatgc cttggtcgcg ggcgtacgcg
    28021 ccgacttcgg cgtgcatggc ggcggcttcg tcctcgccca gttcgcccat atcgcccatc
    28081 acgaaaatgc gcggcgcagg catacgcgcc aacacgtcaa ccgcggcttt catactgtcg
    28141 ggattcgcat tataagtatc gtcaatcagg gttgcgccct tgatgccggc tttgacgttc
    28201 agacggcctt tgatgttgct gaagccttgc aaaccttccg ccacatcgtt caaactcaaa
    28261 ccggcagcca aagccagcgc ggcggcagcg gcggcgttgt ggacattgtg gcggccggga
    28321 acaggcagca ccacggcagt gcgctcgtcg ccgcacacca aatcaaattc gcacgacaaa
    28381 ggtttcagca cgatattttc cgcgcggaca tcgccgctat cgacgccgaa agtgcacgta
    28441 ttcaaattaa acgttgccgt tttgaagaca gccatattgg catcttcttg aggaatcagt
    28501 gccatgccgt ctgaacataa gcctgcataa atctcgcttt tcgctttggc aatatcgccc
    28561 actccgtcga aaccgcatcc gacatgggcg cgcagggcgt tgttgaccaa agcggcatcg
    28621 ggtttggcga tttgcgttaa aaccgccagt tcgccaaaat ggttcatgcc catttcaatc
    28681 acggcatagc ggtgtttttc gtttaatttc aataaagtca gcggcaatcc gatgtggttg
    28741 ttgaagttgc ctgccgtcgc cgaaacggca tcatcgccga aacggcggcg caataccgca
    28801 gccagcatct ccttcaccgt cgtcttgccg cccgaaccgg taatgccgaa cacaaacggg
    28861 ttcacattat cgcgccacgc cttcgccaac gtttgcaacg cggcaagcgt gtcatcgact
    28921 ttcaacgcgc cgcccaaagc cgcgcaatct tcgcgcgaaa ccacaaccgc cgccgcgccc
    28981 gcagacaata cgcctccaac aaaatcatgc gcgtcaaacc gcccgcccgc caatgcgaaa
    29041 aacacatcgc cttcccgaat atcgcggctg tcggttacga tgcgcgacac gggtttgttt
    29101 tcagacggca tcggaagctt gagggcttgg cagatgaaat ttaggtccaa tggtttcata
    29161 tttgctttcg ttaatattcg ggcggcggac acatcggtag cggctgattt ttttatcgcc
    29221 tgttttgctg tggtaaaaca cagattattt tcccattctc attcggcatt ttttctgtac
    29281 gtatcatttt ttagacgtat ttttagccga tttgcctttt cccgcatacc acggcgcggg
    29341 gtcgtcggac tgtctgtcga taaaggcaag gttattgcct tcgcccggca catcggggac
    29401 attcccccaa aaatcatagc cgtcatcggg caactcgtcg gtttcgatac ccgtccaact
    29461 gccgaatccg cgtaaaaaat taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt
    29521 gtcatcggac gaaattttaa ccagcggcac gccgtagctt tgacgcttcc actcgccgtg
    29581 tgtcaacgtt cgctcgccgc ctttgccgac atgcatcaaa ccgtggtcgg aaaagtaaac
    29641 caaagaccag ctttctttat tttcattcaa tatcttaacc gtatcttcta aaaatttatc
    29701 ggtttgcgcg atggtggaaa catagcagga tattttttca gtttgatact gaaaccgccg
    29761 cgcatcctta tccaaacgtg tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat
    29821 cagccgaggc ttcgtgcctg catttcctgt caaaacccgt ttgaacgccg gcaacaaaag
    29881 gcggtcgctc aaccccgcgc ttttgccata atcacccctt tgggtaaacc acggataatc
    29941 gctgcgtagg gcataggtgg aaatttcgtt ggcaaaatgc cccaacattc cttgattaga
    30001 cagccacgcc gtccgaaaac ccgcctgctt cgccaagctg acaatgttat tgttcggctc
    30061 tcccggcagc cccaaagtct gcggcagcga aagattcgtc gcgtgggcgg tcgattggta
    30121 accgtttatc aacagccctt tggtccgact caaaaaaggc gtatcgggca atgggaaacc
    30181 gtaaacattc atataatccg aacgcgcgct ctcaccgata accacaacat aatttttata
    30241 cttgggcgca acatgacgga tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg
    30301 ggcgcgcttg gcggcatatt gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat
    30361 attcaacaac aggccggcat caggttctcg ccaatcttta tcgccggcga ttttctccat
    30421 caccgcgcag gacaaaatca aagtcaataa tatggtcagc catattttgc tgcggcgttt
    30481 atagtttttt acgtcagcaa atacccccac agatacacaa tatttccaaa ctgtcaagcc
    30541 aaaaatgaaa aataatgcct gcacaaaata aagcgacccg ggaagattgc cgacaaattc
    30601 acgcgcctcg gcaggattgc tttccaatat cgaaccgact atctgataag aaggcgcacc
    30661 atacagccag ccgaccggca aatatagggc ggttgtgccg acgtaaatca gtaaaacaac
    30721 tgaagaaacg cgcgggaagc cgcgtgccaa taataataaa aatacaaccg aagccaaagc
    30781 gcccacggca gaataacggt agccgtattc atattccaag tgataacctg atgcgatggc
    30841 cgcgccaaaa caaaaggcta aaagcgcgca aatgcccaat gtatttttta atgtctgatc
    30901 catatttttt attcgggcgg attgtttcat acgatgccgt ccgaatatcc cattatttac
    30961 gagttaacaa agcctgcccg acgatttcaa gatcggaaaa acggtgcttc acgccctgta
    31021 catcctgata gttttcgtgt cctttgccgg caatcaggat gatgtcgttt gcggcggcct
    31081 gctcaaccgc ataacggatg gcggcggcgc ggtcggcttc gacgcattcg ggcgcgggca
    31141 cggcgggcag gatgtcgttg atgatgtcgt gcggattttc caaacgcggg ttgtcgctgg
    31201 ttacgacgac tttatccgcg ccctgtacgg ctgccgcgcc catcaatggg cgtttgccgc
    31261 aatcgcggtt gccgccgcaa ccgaatacgc accataaggc cgcaccctgc ggtttgattt
    31321 cctgcaaagt ggagagtgct ttttccaatg cgtcgggcgt gtgggcgtaa tcgacgacga
    31381 ccaagggctt gccgctgttc atgatgcagt ccatgcgccc tgaagcggga cggatttttg
    31441 ccagcacatc caatacctta tcaagcggat aaccgttggc acaaagcaag gcgatgcagg
    31501 cggcgaggtt ttgcgcgttg aaccgtccga gcaggcgggt gcggcatttt ccttcgcccc
    31561 acggggtttg gaatactgct tccatgccgt ctgaagaggc ggtaaagtcg gtaatgcgga
    31621 tgtcggcgtg ttcgctgaaa ccatagctgt aaacggctaa atcgggacag tcttttttca
    31681 gacgacctgc gagttccgcg ccgtatccgt catcggtatt gataacggcg tgtttcaagc
    31741 cgtgccagta aaacaggcgc gacttgatag caccgtaggc ttccatcgtg ccgtggtagt
    31801 cgaggtggtc tcgggtgagg ttggtgaaga ttgcgctgcg gaacggcacg ccgttgactc
    31861 gcgactggtc aaggccgtgg ctggaaactt ccatcgcggc ggctgttgcg ccttgttgac
    31921 ggaaacggta gagcagggtt tggacatcga cgggggcggg ggtggtgtgc gcggtttctt
    31981 ccaatgcacc ccaaaagccg ttgccgaccg tgccgataat ggcggtttct tcgcccaaca
    32041 aatcggcggc ctgcgccagc cattgcgtga tggaggtttt gccgttggtt ccggttacgc
    32101 cccagacttt gagaccgtct gaaacgttgc cgtaaacctg tgccgccaat atgccggcac
    32161 ggtgtttcaa atctttgatg ccttgattgg ggactttcca ttcgggattc cacgcaaatt
    32221 tgccgtcgtc gtcccaaaaa acaaaagccg cgccgttggc aacggcggcg gggatataac
    32281 tgcgtccgtc ggcatattcg cccggacagg caacgaaaat atcgccttgt ttgatttggc
    32341 ggctgtctga atgcaacaaa tgccctgccg cgtttgcaca cgacagggtc gggatgccgg
    32401 tttcagccaa agggcttaac ttgctgaaca taaaacaatc tcgttgatac tcggattaag
    32461 acggtgtttt gacggctgcg gcagtcaggg gcttggtcgg ggaaatgccc aagatgttca
    32521 ggctgccgcc cataattttt ttgaagggcg gccctgccac tacgccgccg taatagccgt
    32581 tggcagtcgg ttcgtcgatg gttaccgcca caatcacacg ggggtttttg gcgggggcaa
    32641 aaccgataaa ggtagcgacg tgtttgttgt cggcataacg cccgttgacg aacttgcgcg
    32701 ccgtgccggt tttagcgccg acatcgaaac cgtccaccgc acccgccgta ccggtgccgc
    32761 ccggctcggt tacggaaacc atcagattgc gtacctcgcg cgcggtcgat tctttgaata
    32821 tgcgtttgcc ttgcggcgca accgcctgct tctcaaagct gagcggcagc aaaacgccgt
    32881 cgtgcgtcag tgcggtatag gcgcgcgcca attgcagcag gctcaattgc agaccgtaac
    32941 cgaaagacat cgtcgcctgt tcgatgggcc gccacctgcg ccaatttctc aacaaacctg
    33001 cagtttcccc cggaaagccc gagtgcatac gcacaccgat gcccaattca tgatagaagt
    33061 catacatttc ttcggcgccg aaacgcgcag acagtttgct tgtgccgacg ttggacgatt
    33121 tctgcataat gccgcgcaca tccaaagagg ggtaaacatg ggtatcgcgc acgggagacg
    33181 gtccgatttt ataaggctgc gtattcagcc gttcgttcaa atcggttttg cccgcatcca
    33241 atgccttcgc aatcacgaac ggtttgattg ccgaaccagg ttcgatcata tcggttacgg
    33301 cacggttgcg ccgctgttcg ctgtctgccc ggccgggtct gttgggatcg taggcgggcg
    33361 tattggccaa ggcgaggatt tcccccgtgc gggcatccaa aaccaccacc gttccggctt
    33421 ttgcctgatg gtattcgacc gccttgttca actcttcata ggccaaggtc tgaatcctct
    33481 gatcgaggga aaggatgatg tctttgccgt tttgcggtgc tttattgcgc ggggagtcca
    33541 agctgtccac aatattgccc tgccggtccc gcaaaacaac ttccgcgccg tcttcgccat
    33601 acaggctgtc ttcaagcgaa agttccaaac cttcctgacc tttgccgtca atatcggtaa
    33661 atccgatgac gtgtgcaaac aggttgccca tcgggtaatg gcgttttaat tctttttcaa
    33721 atacaaagtt ttccaaaccc aaggctttga cctcttcggc aaccttggga tcgagctgcc
    33781 gcttgatcca aataaacgac ttgcctttct gttcgagttt gttcctcaaa acatcgaccg
    33841 gcacatcgac aagctcggac aggcgttcca attgggcggc agacggcatt tccttcatat
    33901 ctttaggcac ggcaaacagg gactccgtcg gcgcgctcaa cgccaaaacc gcaccgttcc
    33961 ggtccgaaac cgtaccgcgt gtagccggca atgcttgagt ccgcacaatc cggttgtcgc
    34021 cctgttcttt caaaaagtta tacgttaccg tctgcagata cagcccgcgg gcaatcagac
    34081 aggcaaacaa gaccgccatt gccatcagga cgaagctaat ccgtccgtta ctggtcatcg
    34141 gctttttgac ctgctcttct ttgggcagca tccggggctt atattcgctt ttaatcaaca
    34201 tttttacttc tcgttattat tatcctgatg caggaatccg attccggcac acaggctgct
    34261 tctatctttg atgttccacc ataaaggtat tgcccgaaac cggcggatgg aggttttgtt
    34321 tttctgccgc cgccctgatc gcttcgtgat tcgccaaacg cgcctgttgc agcctcattt
    34381 gcgcataatc ctgctccaag gcgatttcct gttttttcgc cttgtccaaa gctgtgaagt
    34441 tgagcctgta ctggttttgc tgcatcacaa cggaaaaagc ggaaacgcac accgcaagca
    34501 gcagaaagaa attcgatttg ttcattgccg ttcagacgtt tttctctgtt attattccgg
    34561 tatcggaccg gcagtccgct ccgccacacg caaaactgcg ctcctcgccc tcgggttggc
    34621 ggcaatttcc gcttcacccg gctttaatgc cctgcccacg attttcaggg gcggctcggg
    34681 caaatccgct tctctgaccg ccgcccagct cggcaggggc tcgtgttgcg aatatttttt
    34741 gacaaactgc ttcacaatgc ggtcttccaa cgaatggaaa gcaatgaccg ccaaacgccc
    34801 gccctctttc agacggcaca tgacctgcgg caataccgcc cctacttctt caagctcgcg
    34861 gttaataaag atgcggattg cctggaaggt gcgcgtcgca ggatcctgcc cccgctcgcg
    34921 agtacggacg ttttgtgcca cgatctgcgc cagcttgcgg gttgtatcga ttggactttc
    34981 cgcccgttgc gcgacaatgg cgcgcacaat ctggcggcta aaccgctctt caccataatt
    35041 cttgattacc tcgtgcaaat cctgttccga cgcaaccgct atccactctg cggcagacat
    35101 accgcgcgtc gtatccatac gcatatccaa aggggcatcg aaacggaagc tgaaaccgcg
    35161 gctgccgtca tcgatttgcg gagacgaaat ccccaaatca aacagcgcac cgtccacctt
    35221 gccgataccc aaaccgtcca atgccgtctg aaacgaagca aaaccgccat gcacgacacc
    35281 gacccgtttg tccgaacgcg ccagctcttc tgccacagca atcgcctgcg ggtctttgtc
    35341 gaaaacaatc aaccgccccg catcgcccaa acgcgacaaa attagccggg aatgccctcc
    35401 cctgccgaac gtaccgtcca catagacacc gtcttcgcgc acggcaagcg catccaccgc
    35461 ctcattcagc aagaccgtga tatgccggta actttctgct ccactcacaa ttgcaaatcc
    35521 gtctgactca actggaaggc aagttcgtca ggatcgtcat ccaaagcctg aaccatctca
    35581 gcctcccact gctcgcgacc ccaaagctcc aaacggttgg cacggccgac caaaacaact
    35641 tcacggtcga aatccaccct tttcctcagt ccggcaggaa ccagcaccct gccggcgctg
    35701 tcccattcca aaatttccgc gttatgcagc aaaagatttt gaaaccgccg caaaacaggg
    35761 ttatccgcca cttttaagtt taaaagttgc gccgcaacct tttcccactc cgcaacaggg
    35821 tacatcaaca gcttatgttt cgactcgagc gttgccacta cggcaggcgt atagaggcgc
    35881 gacagaatgt cgcggaattt ggcaggaaca gccaaccgcc ccttactgtc gatgcttaat
    35941 tcgtgtgcgc cgccgaacat gacatgtccc aagccgaaat caaaatcaca agggtaaaag
    36001 agacactttg ccccacaatt ccccaccaat cgacactata agaaatttta aacactcggt
    36061 caaatcaggg catgaaaact cattaacata tctgaaattt ttattctgtt taaaaacaat
    36121 aagataaaaa atgacgacaa cggcacggcg ggtgcagtac agagtgaccg aaccaataaa
    36181 caactatata tgattaattt aataatataa acacaatata tagtattaag ataaagccat
    36241 gacagcaccc gtaccaacgt gtaatatgtc gggaaatcca ataaatttac acaagctaac
    36301 atttatcatg ctcctcccct cccccgaagc acggcaatcc tcgctcaatc tgcaaaccct
    36361 cattgccgaa gaaatcggca aacacggcaa ttggattcca ttttcacgtt ttatggaatt
    36421 ggttttatac gctccgcaat acggctacta caccggcggc agccataaaa tcggcaatac
    36481 cggggatttt attaccgcac cgaccctcac ccctctgttt gcacagacac tggcacgcca
    36541 acttcaagaa ctcctgcccc aaacggcggg caatatctat gaattcggcg cgggaaccgg
    36601 acagcttgcc gccgatttgt tgggcagcgt ttcagacagc atcaactgtt actatattat
    36661 tgaaatatcg ccggagctgg cagcacgtca gaaaaacctg attcaagccc gtgcaccgga
    36721 agcatctcaa aaagttgtcc acttgaccgc acttcccgaa gcgtttgacg gcatcatcat
    36781 cggcaacgaa ttactcgatg ccatacctgt cgaaatcgtc cgtaaaaatg aaggaggatt
    36841 actcgagcac atcggcgttt gcacggataa cggccgtttc gcctattctg caagaccctt
    36901 gcacgacccg tcgctttcca cctcggcttc cctctatttt cctcaaacag attatcccta
    36961 taccagcgaa ctgcacccgc aacaatatgc ctttatccgc acccttgcct caaggcttga
    37021 acgcgggggt atgatattca tcgactacgg ttttgatgcg gcgcagtatt accacccgca
    37081 acgcaatcaa ggcacattga tcggacacta ccgacaccac gttatccaca atcctttcga
    37141 cttcatcggg ttggcagatc tgaccgccca cgtcaacttt accgacattg cccaagccgg
    37201 tacggatgca ggactggatt tgacaggcta tctgccccag tcccattttc tgctgaatct
    37261 gggcataacc gaactgttgg cacagacggg taaaacagat tcggcagcct atatccgtga
    37321 agccgccgct gttcaaaaac tgattgacca gcatgaaatg ggcgaactgt ttaaagtcat
    37381 cgcattcgga aaaaatatcg gcatcgactg ggcaggattc cgcttcggcg acatctgcca
    37441 caaactccaa ccctcgtgcc gtctgaatcc gcttcagacg gcataaactt tttaacattt
    37501 aaaaacaaga aactaattca aaattaaaaa atacggcttg tcaaaaaaac aaaaaaacat
    37561 ataatagcta attcacgaaa cggcgaatta gctcagtcgg ttagagcaga ggaatcataa
    37621 tccttgtgtc cggggttcga gtccctgatt cgctaccaga ttttcggggg tatagctcag
    37681 ttggtagagc gcttgcatgg catgcaagag gtcagcggtt cgatcccgct tacctccacc
    37741 agataaaaaa gcacagaccg taaaaggtat gtgctttttt attgcctgat tgccggaaaa
    37801 taaagaataa accactgcct tcaaaacagg caatcgactt taaacctatt gccccgcctg
    37861 tcctgatttt atcagcccgc cagacaaacc cgacctgaat aatgtcttca ggtcgggttt
    37921 atggtttcat tcccaactta tccagcccga caaccacaat attatgatga caatgccgaa
    37981 aacaatgcgg taataggcaa acgggatata gtttttcttg gaaacaaact tcagcagtgc
    38041 tttaaccgct accaaaccgg aaacaaaagc ggcaataaag cctatcaaaa tcaaaccgac
    38101 atcatgcagg gtgaaaaatc ggtaatgttt caggacatca taagccgttg ctgcaaccat
    38161 catcggaacg gccaagaaaa atgaaaactc cgttgccgtt ttccgctcga ttccccaaaa
    38221 catcccgccc ataaccgtac tgcccgaacg ggacgtaccc ggaaccagtg caaacacttg
    38281 ggcaacaccg atcatcaacg catcaatcgg acgcaatgca tcaacatcgg caattttagg
    38341 ctctgctcgg ctttggcgtt tctccaccca caaaataaaa aaaccgccca aaaccagcat
    38401 gactgcaaca ctcaaggggt taaacagata ctctttgatt tgtttgtcga acaacagccc
    38461 catcacggcg gcaggtataa aagcaatggc aagattgagg acgaaacggt tggctttccg
    38521 gtcttttccc acgccatgca acacattgct gaaacgctgc cggtattcaa acactaccgc
    38581 caaaaccgca ccgagctgga tggcaatttc aaaaaccttg tgattgctgt gaaaaccaat
    38641 cagattgccg aacacaatca aatgtccggt gctggaaatc ggtaaaaatt cggtaaaacc
    38701 ttctaccaag cccatcatca gggctttcag gacaatcaga aaatccattg cttgcgcttc
    38761 tttcggatac gggagttcgg ctatttctgt acagcagggg tctgacgctt gcgttcttcc
    38821 ctgactttgg caaccaattc tttaatcgta tgaacgccgc catcaaagcc attattgaag
    38881 ataacgcggt attttccgcc gacaataacg gtcggcgtgc tgtcaatacc gtattgttcc
    38941 gtcagtttct gcatttttaa tgcgacggcg gcagcttcgg gggaatcata ggcgcgcatc
    39001 agttttttgc cgtcaaaacc tttttgagat aaagcccatt ttccggcaac agccctgttt
    39061 tccaaacgga ttttttgttc gtaaactgct ttaaacacag cagagtttgc ctgatatttc
    39121 aaacccgaca gcttgaccgc agcagccatt cttgccagac cgagcatttc aggccgccag
    39181 accacgtgct ccgtccgcag ataagtatca gacggcaatg ccttgcccag tttcaataac
    39241 aaaggatcga aatgatggca atgtacgcaa aaatagccga aaaattccaa aacctcaatt
    39301 tttcccggct gttcttgagg aatgggttta tccaacacaa gatagtcttc cccttccgtc
    39361 agggcatatg cctgcgcgga caacactgcc gacagcagca gcggcaacag atgtttaaac
    39421 ttcataatta tttgccttca atcgcacgga tcaggctggc aaccccatgc tttttcaact
    39481 cgtcctgcat ttttttcacc gcatcggcag acatattgcc gctttgcacg cggtaaagcg
    39541 ttttatgtcc cgcctgatag ccgaccactt cggaagatat gcccaagatt gccagtttgg
    39601 cacgctgccc ttccgcgctc cggcggtcgg catacgcgcc catttgcaga taatgcgttg
    39661 cttccgcctt gccaaacgtt ttcattttct gcacttcttt ggcagcggca ctgcgcgctt
    39721 tttcgatgct gccgctgttg aggatttgtt ccggggtcgg tttgggtgca actttttcct
    39781 tcgccgcctt tttctcttct tttgaagctt ttttctctgt ttctttagac ggttttaccg
    39841 cttgtttttt aaccgtttcg gcatctttct tctgcgcttt ttccctgacg gtttgttcac
    39901 gctcttccgt cagtgctttc ttgcgcactg cctgtccgtc cggctcttcc cgttccggct
    39961 cgcccgcctt ttcttcaacc tcgtcggctt tgtcggcaac gggctgcttg tcggcagctt
    40021 tttccgcatc cgactgctct gcctctttcg caacatccga ttcggacaag gcgttttgat
    40081 cggccggttc aggttggatg tcttccttag gctggttttt cagtttcagg atttccgttt
    40141 ctgcaggctg cttcgacgga gccgggattt tgaacgcatt ttgaccgccc tggttcagat
    40201 aaagcaaaat accggcaata atgaccgttg ccagtatcaa accgaagaag aaaccggaca
    40261 gaccttttcc ggattgggaa aatttgttca taaacatacc ttaatgtgtt tcagacggca
    40321 ttaacgccgt ttgcttacgg gcggatattc taacaatatc gccatatttg ggcaaaacct
    40381 gcttccattc ccgttcctat aaagcacgac ggaagcctat ggctttcatg acgtggcttc
    40441 tgccgacttc ttcatcgccc gccaaatccg ccaatgtacg cgccacgcgc ataatgcggt
    40501 ggaagctgcg ggcggaaaga gacaattttt ccagcaggct acccaaggct tcctgcgctt
    40561 ctttttggat gcgggcttgg gaatcgagtt cgctgacgct caaagcggcg ttgactttgc
    40621 cctgccgtgc gtattgtttg ccgcgggcgg cgatgacgcg ttccaaaacg gacgcactgc
    40681 tttcgcccgc ttcctgctgc atcagttcgg cggcggacag gctggggact tcgatggtca
    40741 aatcgatgcg gtcgagcagc ggaccggaaa ttttgcttcg gtaacgcgcg acactttcgg
    40801 gcgtgcagcg gcagggtttg acgggatgcc cgagataacc gcacggacac gggttcatgg
    40861 cagcgacaag ctggaatttg gcgggataga cggcttggcg cgccgcgcgg gaaatgtgga
    40921 tttcgccgtt ttccaacggt tcgcgcaaaa cctccaaaac tttgcggtcg aactcgggca
    40981 attcgtccaa aaataaaacg ccgtgatgcg ccaatgaaat ctcgcccgga cgcggatccg
    41041 agccgccgcc gaccatagcc gccgcgctgg cgctgtgatg cggactgcgg aaaggacggt
    41101 tgctgtcgag ttgttgttgg tggttgggca ggagcgaacg caatgcccaa acttctacca
    41161 attcgtcttc ggccagcggc ggcaggatgc cgggcagccg ttgggagagc atagacttgc
    41221 ctgttcccgg cgggcccatc atcaagaggc tgtgtccgcc tgcggcggcg atttccaaag
    41281 caaggcgggc agtgtgctga cctttcacat cacacaaatc aggttgtccg ccatgttcag
    41341 acggcatttg cggtacggaa cattcggttt gcgccaaagg ttcgataccg ttcaaatgag
    41401 cggcgacttc gcccaaagag cgcgcgccgt aaactgtaat gccgcgcatc acggcggctt
    41461 gtcctgcgtt ttcttcaggc aaaacaaatg cacgttttgc ctgcataccc tgccacgcca
    41521 tcgccaacgc gccgcgcacg gggcgcaaca accccgacag tgccaattcc cccgcaaact
    41581 cgtattccgc cagcttttcg ggtgcaacct gccccgatgc ggcaaggatg ccgattgcta
    41641 tcggcaaatc gaaacgcccc gactctttgg gcaggtcggc aggggcgagg ttgacggtga
    41701 tttttttggc ggggaattca aaaccgcttt gaataatggc ggcacggacg cggtcgcggc
    41761 tttcctttac ttccgtatcg ggcagtccga cgatgttgaa atgcggcaga ccgttggcaa
    41821 ggtgggcttc cacttcgacc aacggcgcat tcataccgct caaggcgcgg ctgtaaacca
    41881 aggcaagcga catatttcag acggccttat tcgccggctt cggtttgctg cctgatttcg
    41941 gcgacggctt cttcggcagc ggcttcagcc gcttccaatg ctgcccgttc gggattttgc
    42001 gcggcttcga gtctcgccag acgtgcttcc aaagccgcca gtttggtacg ggttttaatc
    42061 aaaacctgct gctggatgtc gaattcttcg cgcgtaacca aatccatacg gttgaacgcg
    42121 ccgcccagca tcgccttaat atttttttcc acatctttgg cagggctgtt ggcgatggtt
    42181 tcgctgattt tcgagccgac ttcttcaaaa agctgcttgc cgaacataat ctgcatcctt
    42241 cctgaacata tcaaattcaa gcggctattg tataaggaaa aatgccgtct gaaaacgggc
    42301 ggcggataat cgtcaaaaca taccgcgccc cctttgcggt tgctaaaatt acacttcgag
    42361 acacctgccg cctgaaaacg gatttcatat tccgcgcaac gcccgattac attacaagac
    42421 actacaaaac aatatgctgt tttaaatgat ttttccgatg cgcatcgttt caaactcggc
    42481 tttttaagcc attaagtgct ttgcaaacaa cagaaattgg gttatgctga aacggattat
    42541 ttacaatttc atatagtttc attacatatc ttattgtaat tgaagataat ttatccgaat
    42601 cccctttcgg gtatccggat tttccgttgt actttcatta gaaaaacatt tcaggcgcaa
    42661 gttgcttgca atttcaaagc ctgtaattct gaagttttag acgaggaata gcacatgaat
    42721 cccatgtaca tcacttttgc aatctatttg gttgcagtcc ttctcatcgg gcttgccgct
    42781 tatttttcca cgcgcaattt cgatgattat attttgggcg ggcgcagcct gggcccgttt
    42841 gttacggcga tgtcggcagg cgcgtccgat atgtccggct ggcttttgat gggtctgccg
    42901 ggcgcgattt atttgagcgg tttgaatgag gcttggattg ccatcggcct cttggtcggc
    42961 gcgtatttca actggctctt ggtggcgggc cgcctgcgcg tgcataccga atatgccaac
    43021 aatgcgctga cgctgccgga ttatttcttc caccgcttcg gcgcgggcgg acacttgatg
    43081 aaagtggttt ccgcactgat tatcctgttt ttcttcacga tttattgcgc ctcgggcatt
    43141 gtggcgggcg caaccctgtt ccaaagcctg tttgaaggta tgacttacaa tcaggcaatg
    43201 tggctgggcg cgggcgcgac catcgcctat accttcttgg gcggcttctt ggcggtcagc
    43261 tggacggata cgctgcaggc ttctttgatg attttcgccc tgattttaac gccggtgatg
    43321 gtctatctgg gcttgggcgg cgcggaacag atgtctgccg cgattcaaag cgtcgccgca
    43381 ggcacgggca aagaatacgg cagcctgttt gccggtacga ccgtcatcgg catcatttcc
    43441 accgccgcct ggggcttggg ctatttcgga cagccgcaca ttttggcgcg ctttatggcc
    43501 gccgaaagcg cgaaatcgct ggtatccgca cgccgcatcg gcatgacttg gatggcgttg
    43561 tgccttgcgg gcgcggtagc ggtcggttat ttcggcattg cgtatttcgg tgcaaacccc
    43621 gacaaagttt cttctatgag cggcaaccac gaacgcatct tcatcgcgct ttccaccctg
    43681 ctgttcaacc cttggatcgc cggcatcatt ttgagcgcga ttttggcggc ggtgatgtcc
    43741 accctgtcct gccagctttt ggtttgctcc agcgcgatta ccgaagactt ctacaaaggc
    43801 tttttgcgta aaaacgcgca acagtcggaa ttggtatggg tcggccgcct gatggtattg
    43861 gcgattgccg tgatttccat cctgattgct tccgatccga acagcaaagt attgggactg
    43921 gtgtcttacg cgtgggcagg tttcggtgcg gcattcggcc cgattgtgat tctgtccgta
    43981 ttgtggaaac gcatcactgc ctacggcgcg ttgtcaggca tggttgcagg tgcgtcaacc
    44041 gttgttgtgt gggcggaatg ggtaaaaaaa ccggctcgag cggcaggcga aagcggcttg
    44101 ttgacgatgt acgaaatcgt cccgggcttt attgtttgct tgatcgttgc cgtgttggtt
    44161 tccctgttca acaaagaacc ttcgcgcgaa atccaagaac gctttgaaaa agccgacgca
    44221 gattaccgcg ccgcccgata aacccctttc agacggcacg gcatcccggt tcgtgccgtc
    44281 tgaacccgct tccgggccaa acacaaataa accttgcctg catacaggcc ttatttgtgt
    44341 tttacccgtt tcattttcaa agaaacggaa gcaccgattc tgcccgcatt tgaagccttg
    44401 tatttcatct gcggatttat tacgatatta ccgtattcag gccgcaccga tgccgtctga
    44461 accccccgaa aaaactttgg aggatccaaa aaatgtttca ttttgcattt ccggcacaaa
    44521 ctgccctgcg ccaagcgata accgatgcct accgccgtaa tgaaatcgaa gccgtacagg
    44581 atatgttgca acgtgcacag atgagtgacg aagagcgcaa cgccgcctcc gagcttgccc
    44641 gccgtttggt tacccaagtc cgcgccggcc gcaccaaagc cggcggcgtg gatgcgctga
    44701 tgcacgagtt ttcactctcc agcgaagaag gcatcgcgct gatgtgtctg gcagaagccc
    44761 tgctgcgtat ccccgacaac gccacgcgcg accgcctgat tgccgacaag atttcagacg
    44821 gcaactggaa aagccatttg aacaacagcc cttccctctt cgtcaatgct gccgcctggg
    44881 gtctgctgat taccggcaag ctgaccgcca caaacgacaa acaaatgagt tccgcactcg
    44941 gccgcctgat cggcaaaggc ggcgcaccgc tcatccgcca aggcgtaaat tacgccatgc
    45001 ggcttctggg caaacagttc gtcaccggac agaccattga agaagccctg caaaacggca
    45061 aagaacgcga aaaaatgggc taccgcttct ccttcgatat gttgggcgaa gccgcctaca
    45121 cccaagccga tgccgaccgc tactaccgcg actatgtcga agccatccac gccatcggca
    45181 aagatgcggc aggacaaggc gtttacgaag gcaacggcat ttccgtcaaa ctctccgcca
    45241 tccatccgcg ctactcgcgc gcccaacacg gccgcgtgat gggcgaactg ttgcctcgcc
    45301 tgaaagaatt gttccttttg ggtaaaaaat acgatatcgg tatcaacatc gatgccgaag
    45361 aagccaaccg tctggagctg tctttggatt tgatggaggc actcgtttcc gaccccgatt
    45421 tggccggcta caaaggcatc ggtttcgttg ttcaagccta ccaaaaacgc tgcccgttcg
    45481 tcatcgacta tctgattgac ctggcccgcc gcaacaacca aaaactgatg atccgcctcg
    45541 tcaaaggcgc gtattgggac agcgaaatca aatgggcgca agtggacggc ttgaacggct
    45601 atccgaccta cacccgcaaa atccataccg acatctccta cctcgcctgc gcgtgcaaac
    45661 tgctttccgc gcaagacgcg gtattcccgc aattcgccac ccacaacgcc tacaccttgg
    45721 gcgcaatcta ccaaatgggc aaaggcaaag attttgaaca ccaatgcctg cacggtatgg
    45781 gcgaaacctt gtacgaccaa gtcgtcggtc cgcaaaactt aggccgccgc gtgcgcgtgt
    45841 acgccccggt cggcacacac gaaaccctgc tcgcctactt ggtgcgccgc ctgttggaaa
    45901 acggcgcgaa ctcgtctttc gtcaaccaaa tcgttgatga aaacatcagc atcgaccgcc
    45961 tgattaagag cccgttcgac accatcgccg aacaaggcat ccacctgcac aacgccctgc
    46021 cgctgccgcg cgatttgtac ggcaaatgcc gtctgaactc gcaaggcgtg gacttgagca
    46081 acgaaaacgt attgcagcag cttcaagaac agatgaacaa agccgccgca caagacttcc
    46141 acgccgcatc catcgtcaac ggcaaagccc gcgatgtcgg cgaagcgcaa ccgattaaaa
    46201 accctgccga ccacggcgat gttgtcggta cggtcagctt tgccgatgcc gcgcttgccc
    46261 aagaagcggt tggtgcagcc gttgccgcgt tccccgaatg gagtgcgaca cctgccgccg
    46321 aacgcgccgc ctgcctgcgc cgtttcgccg acctgctcga acagcacacc cccgcgctga
    46381 tgatgcttgc cgtgcgcgaa gcgggcaaaa cgctgaacaa cgccattgcc gaagtgcgcg
    46441 aagccgtcga tttctgccgc tactacgcaa acgaagccga acacaccctg cctcaagacg
    46501 caaaagccgt cggcgcgatt gtcgccatca gcccgtggaa cttcccgctc gccatcttta
    46561 ccggcgaagt cgtgtccgcc ctggcggcag gcaacaccgt catcgccaaa cctgccgaac
    46621 aaaccagcct gattgccggt tatgccgttt ccctcatgca cgaagccggc atcccgactt
    46681 ccgccctgca actcatcctc ggcgcaggcg acacgggtgc ggcattgacc aacgatgcca
    46741 gcatcggcgg cgtgattttc acaggctcga ccgaagtggc gcgcctgatc aacaaagccc
    46801 ttgccaaacg cggcgacaat cccgtcctga ttgccgaaac cggcggacaa aacgccatga
    46861 ttgtcgattc caccgcactt gccgaacaag tctgcgccga cgtattgaac tccgccttcg
    46921 acagcgcggg acaacgctgc tccgccctgc gtatcttatg cgtccaagaa gacgttgccg
    46981 accgtatgct cgacatgatc aaaggcgcga tggacgaact cgtcgtcggc aaaccgattc
    47041 agctcactac cgatgtcggt cccgtcatcg atgccgaagc gcagcaaaac ctgttgaacc
    47101 acatcaacaa aatgaaaggc gttgccaagt cttaccacga agtcaaagcc gccgccgatg
    47161 tcgattccga aaaatccacg ttcgttcgcc ccatcctgtt tgaattgaac aacctcaacg
    47221 aactgcaacg cgaagtcttc ggccccgtcc tgcacgtcgt ccgctaccgc gccgacgaac
    47281 tcgacagcgt catcgaccaa atcaacagca aaggctacgc cctgacccac ggcgtacaca
    47341 gccgcatcga aggcacggta cgccacatcc gcagccgcat cgaagccggc aacgtttacg
    47401 tcaaccgcaa catcgtcggt gcagtcgtcg gcgtacagcc cttcggcgga cacggtctgt
    47461 ccggcacagg ccccaaagca ggaggttcgt tctacctgca aaaactgacc cgcatccccg
    47521 aatgggttgc cccgaccctg agccaaatcg gacaggcaga cgaagccgcg ctcaaacgcc
    47581 tcgaagccct gattcacaaa ctgccgttca acgccgaaga gaaaaaagcc gcagcggccg
    47641 ctttgggaca cgcccgcatc cgcaccctgc gccgtgccga aaccgtcctt accggcccga
    47701 ccggcgagcg caacagcatc tcatggcacg cgccaaaacg cgtttggata cacggcggca
    47761 gcacggttca agcctttgcc gcactgaccg gacttgccgc ctccggcgta caggcagtgg
    47821 tcgaacccga cagcccgctg gcttcctaca ccgccgactt ggacggtttg ctgcaaatca
    47881 gcggcaaacc cgaaaacacc ggtgtgaacc acgtcgccgc cctaagccca ctggacagca
    47941 gccgcaagca ggaactggca aaccgcgacg gcgcactcat ccgcatcctc ccgtccggac
    48001 aaggcttgga catcctgcaa gtgtttgaag aaatttcttg cagcatcaac accacagccg
    48061 ccggcggcaa cgccggcctg atggcggttg ccgactgatt ttgccaaacc gcccgggcgc
    48121 ggcccgtgaa caaatgccgt ctgaaaacct ttcaggcggc attttatagt ggattaacaa
    48181 aaatcaggac aaggcggcga gccgcagaca gtacaaatag tacggcaagg cgggccaacg
    48241 ctgtactggt ttaaattcaa ttcactatat gtgttggcac atcgctccaa acctgatata
    48301 atccgccttt caacatcagt gaaaatcgtt ctttttagtc agttaacatt aaatttcgga
    48361 gtcg
//
LOCUS       DAXFHF010000006        47360 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXFHF010000006 DAXFHF010000000
VERSION     DAXFHF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 47360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47360
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.006"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            29..241
                     /locus_tag="ACFIRM_000273"
     CDS             29..241
                     /locus_tag="ACFIRM_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3027998.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            426..926
                     /locus_tag="ACFIRM_000274"
                     /pseudo
     CDS             426..926
                     /locus_tag="ACFIRM_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            923..1249
                     /locus_tag="ACFIRM_000275"
     CDS             923..1249
                     /locus_tag="ACFIRM_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC3027999.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1270..1980
                     /locus_tag="ACFIRM_000276"
                     /pseudo
     CDS             1270..1980
                     /locus_tag="ACFIRM_000276"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            2156..2719
                     /locus_tag="ACFIRM_000277"
     CDS             2156..2719
                     /locus_tag="ACFIRM_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC3028000.1"
                     /translation="MNSTVPANNSPLQHALAKLTERETAAVSRQLDPARCDPGFLPFH
                     AFARSIGTEEGWDFAETDEARRNLIAGFAEIHARKGTPYAIRALFPILRLGEIQIIER
                     DGEFKWDGSVLFDGSRTFGRREGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCEL
                     TALDYRNHPHRWNGKIRFNGEYGFGTT"
     gene            2759..5010
                     /locus_tag="ACFIRM_000278"
                     /pseudo
     CDS             2759..5010
                     /locus_tag="ACFIRM_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(5672..5983)
                     /locus_tag="ACFIRM_000279"
     CDS             complement(5672..5983)
                     /locus_tag="ACFIRM_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC3028001.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5991..6270)
                     /locus_tag="ACFIRM_000280"
                     /pseudo
     CDS             complement(5991..6270)
                     /locus_tag="ACFIRM_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6355..6968
                     /locus_tag="ACFIRM_000281"
                     /pseudo
     CDS             6355..6968
                     /locus_tag="ACFIRM_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6947..7111
                     /locus_tag="ACFIRM_000282"
                     /pseudo
     CDS             6947..7111
                     /locus_tag="ACFIRM_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            7213..7347
                     /locus_tag="ACFIRM_000283"
                     /pseudo
     CDS             7213..7347
                     /locus_tag="ACFIRM_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            complement(7431..7868)
                     /locus_tag="ACFIRM_000284"
     CDS             complement(7431..7868)
                     /locus_tag="ACFIRM_000284"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3028002.1"
                     /translation="MPFVAFGLCRYRISEKGSNDLLSRRAVPKPRSKQLGRDYSRSAG
                     LHPVGDTVADALADAKAAAMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVE
                     ADDAALTGQVRFNVSWPQHILNRVDACTAARHETRAVFWRKPP"
     gene            complement(7918..8073)
                     /locus_tag="ACFIRM_000285"
     CDS             complement(7918..8073)
                     /locus_tag="ACFIRM_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028003.1"
                     /translation="MAETVMPEYRNWQNGLDAKCADGDEYGVAQSDCRTREINAKTKE
                     IQGYLID"
     gene            complement(8103..8555)
                     /locus_tag="ACFIRM_000286"
     CDS             complement(8103..8555)
                     /locus_tag="ACFIRM_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC3028004.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            complement(9333..10979)
                     /gene="pgi"
                     /locus_tag="ACFIRM_000287"
     CDS             complement(9333..10979)
                     /gene="pgi"
                     /locus_tag="ACFIRM_000287"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3028005.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(11205..12053)
                     /gene="hexR"
                     /locus_tag="ACFIRM_000288"
     CDS             complement(11205..12053)
                     /gene="hexR"
                     /locus_tag="ACFIRM_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC3028006.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(12102..13088)
                     /locus_tag="ACFIRM_000289"
     CDS             complement(12102..13088)
                     /locus_tag="ACFIRM_000289"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC3028007.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(13069..13764)
                     /gene="pgl"
                     /locus_tag="ACFIRM_000290"
     CDS             complement(13069..13764)
                     /gene="pgl"
                     /locus_tag="ACFIRM_000290"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC3028008.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(13915..15360)
                     /gene="zwf"
                     /locus_tag="ACFIRM_000291"
     CDS             complement(13915..15360)
                     /gene="zwf"
                     /locus_tag="ACFIRM_000291"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC3028009.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSKQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            15392..15523
                     /locus_tag="ACFIRM_000292"
     CDS             15392..15523
                     /locus_tag="ACFIRM_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028010.1"
                     /translation="MPSETCFFPSRSIAISETKAGGIMSSNTTHRYTFCLFPFLQFI"
     gene            15679..15797
                     /locus_tag="ACFIRM_000293"
                     /pseudo
     CDS             15679..15797
                     /locus_tag="ACFIRM_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            15881..17716
                     /gene="edd"
                     /locus_tag="ACFIRM_000294"
     CDS             15881..17716
                     /gene="edd"
                     /locus_tag="ACFIRM_000294"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC3028011.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            17925..18563
                     /locus_tag="ACFIRM_000295"
     CDS             17925..18563
                     /locus_tag="ACFIRM_000295"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC3028012.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(18752..18937)
                     /locus_tag="ACFIRM_000296"
     CDS             complement(18752..18937)
                     /locus_tag="ACFIRM_000296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028013.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(18948..19060)
                     /locus_tag="ACFIRM_000297"
                     /pseudo
     CDS             complement(18948..19060)
                     /locus_tag="ACFIRM_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19158..20198)
                     /locus_tag="ACFIRM_000298"
     CDS             complement(19158..20198)
                     /locus_tag="ACFIRM_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC3028014.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            20361..21401
                     /gene="mutY"
                     /locus_tag="ACFIRM_000299"
     CDS             20361..21401
                     /gene="mutY"
                     /locus_tag="ACFIRM_000299"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC3028015.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            complement(21777..22145)
                     /locus_tag="ACFIRM_000300"
     CDS             complement(21777..22145)
                     /locus_tag="ACFIRM_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028016.1"
                     /translation="MYDKIHKSCRFFAGSFCFMFGAVPDNRVKFPLAHYFKIEILYFI
                     SLLLKRCSHFFIGFIIHHLIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGA
                     IIHLLEFVAIGADSCDDYVE"
     gene            complement(22163..23905)
                     /locus_tag="ACFIRM_000301"
                     /pseudo
     CDS             complement(22163..23905)
                     /locus_tag="ACFIRM_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(24123..30197)
                     /locus_tag="ACFIRM_000302"
                     /pseudo
     CDS             complement(24123..30197)
                     /locus_tag="ACFIRM_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            30398..30649
                     /locus_tag="ACFIRM_000303"
                     /pseudo
     CDS             30398..30649
                     /locus_tag="ACFIRM_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30647..30841)
                     /locus_tag="ACFIRM_000304"
                     /pseudo
     CDS             complement(30647..30841)
                     /locus_tag="ACFIRM_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            31235..32572
                     /gene="ribBA"
                     /locus_tag="ACFIRM_000305"
     CDS             31235..32572
                     /gene="ribBA"
                     /locus_tag="ACFIRM_000305"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC3028017.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(32735..32851)
                     /locus_tag="ACFIRM_000306"
     CDS             complement(32735..32851)
                     /locus_tag="ACFIRM_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC3028018.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(32883..33959)
                     /locus_tag="ACFIRM_000307"
     CDS             complement(32883..33959)
                     /locus_tag="ACFIRM_000307"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC3028019.1"
                     /translation="MIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVDV
                     YDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQGN
                     TILSPYHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLESDENRERFFAGIPKIKAKDK
                     DKMEIYQLFIQHILFSLKEDGKAAIVLPTGFITAQSDIDKKIREYLVENKMLAGVVSM
                     PSNIFATTGTNVSILFIDKTNKDKVVLIDASGLGEKIKDGKNQKTVLSCEEEQKICNT
                     FTNKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADLD
                     KLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(33963..34484)
                     /locus_tag="ACFIRM_000308"
     CDS             complement(33963..34484)
                     /locus_tag="ACFIRM_000308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFC3028020.1"
                     /translation="MTEQHFTEQTKALIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIF"
     gene            complement(34551..35393)
                     /locus_tag="ACFIRM_000309"
     CDS             complement(34551..35393)
                     /locus_tag="ACFIRM_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028021.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANS
                     VQPI"
     gene            complement(35404..35964)
                     /locus_tag="ACFIRM_000310"
     CDS             complement(35404..35964)
                     /locus_tag="ACFIRM_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028022.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            complement(35957..37159)
                     /locus_tag="ACFIRM_000311"
     CDS             complement(35957..37159)
                     /locus_tag="ACFIRM_000311"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3028023.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            complement(37209..37439)
                     /locus_tag="ACFIRM_000312"
                     /pseudo
     CDS             complement(37209..37439)
                     /locus_tag="ACFIRM_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(37489..40493)
                     /locus_tag="ACFIRM_000313"
                     /pseudo
     CDS             complement(37489..40493)
                     /locus_tag="ACFIRM_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(40613..41923)
                     /locus_tag="ACFIRM_000314"
     CDS             complement(40613..41923)
                     /locus_tag="ACFIRM_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC3028024.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLASA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            42120..42421
                     /locus_tag="ACFIRM_000315"
                     /pseudo
     CDS             42120..42421
                     /locus_tag="ACFIRM_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43227..43754
                     /locus_tag="ACFIRM_000316"
     CDS             43227..43754
                     /locus_tag="ACFIRM_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028025.1"
                     /translation="MNQEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTG
                     ARPGTRDDGNKIILEAGGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAY
                     GDKSLVKLIGENNIVKSEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIW
                     GLYSNNSSISLKGKK"
     gene            43872..45476
                     /locus_tag="ACFIRM_000317"
     CDS             43872..45476
                     /locus_tag="ACFIRM_000317"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3028026.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGININLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            45789..45983
                     /locus_tag="ACFIRM_000318"
                     /pseudo
     CDS             45789..45983
                     /locus_tag="ACFIRM_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(46004..46920)
                     /locus_tag="ACFIRM_000319"
                     /pseudo
     CDS             complement(46004..46920)
                     /locus_tag="ACFIRM_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            47175..>47360
                     /locus_tag="ACFIRM_000320"
     CDS             47175..>47360
                     /locus_tag="ACFIRM_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC3028027.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIG"
BASE COUNT        11722 a        12243 c        11430 g        11965 t
ORIGIN      
        1 tatgcgaaac aaaataaacc gcaacgacat ggaactcggc tacacgcctt acaaccttcg
       61 caccctgcgc aaccgctgca aactgacaca ggccgagttg gcgcaaatcg tcggggtgaa
      121 gcactacata caggtaggcc gctgggaagc cgaacccgat accgaaacgc ggcgggccga
      181 tatgccgctg gaaaaatggc ggcagttcct cgattggata gaaaaaacaa acgccgtctg
      241 aaaccttcag acggcatttt ttacccaccc ccaaacctaa aaaccacaac caagccctac
      301 accgcttgcc cccacctcgc cgccgcccca aaataacctc actctgttgg aagcctgccg
      361 gaaacggcaa aagccgcccc gttccgccat tacggggcgg tttcccacat caagacgaga
      421 cagaaatgac cgaccgccaa atagacaacc tcatcaaacc cgccaccatc gccgacaccg
      481 accccgcgtc cgcgcacaac acggcggcat taccaccgac tggctgccct atatcgtccc
      541 ctttgcaggc ggcgtgtcag tatggcgcat cccaagtgtc ggcgaagcct gcaccatctt
      601 atcccctgcc ggcgaacccg agaacggcgt agttttatgc tgccaagcct ccgaccgata
      661 ccccgccccg tccgccgacc ccgccgaaac cgtcgtccga tttcatccgc tacaaccaca
      721 acagcggcgc gatggaatta aaagccgtta caagcctgac catcgacacc ccccaaacca
      781 ccataaccgg acacctgacc gtcaaccaaa ccaccaccgc ccaagggctg ctgacctacc
      841 aaaatggcat gaacggccaa ggcggcagcc tgtccgagca cacccacccc gacgactccg
      901 gcggtacaac ggaaaaaccc caatgaccga cgcagaaaac ggacgcgggc aagacaccct
      961 cgcccacatc gcccaatcca tccgcaacat cctgttcaca agaataggaa cgcggctgat
     1021 gcgcgaagaa tacggcagtt ttattcccga cttgatcgat atgcccgccg gtcacgccgc
     1081 catcgccctc atccaccaag ccgccgttac cgccctcgca agatggaaac cccgcataac
     1141 cgtccgccgc atacaggcag acaccgccga cctcgccgcc ggcaaaatca aattaaccct
     1201 agacgtaacc cttgccgacg gcggcgaacg aacctaccgc atcaaataat aaaaacaaca
     1261 ggataaacaa tgggaaacag ccgattaagc caactccccg cgcccgccgc catcgaagaa
     1321 accgactttg agggcatctt cgcgcgcaaa aaagccgccc tcaccgccct atgtcccgaa
     1381 agcatccgcg aaaccgtcgc ccaaaccctc gaattagaat cagaacccct aaccatcgac
     1441 ctgcaacagc aagcctatca agagctgctc gtccgcaacc gcatcaacga agccgtcaaa
     1501 gccaacctct tggcatacgc acaaggcagc gacctcgacc acatcgccgc ccaatacgga
     1561 ctttcacgca aaaccatccg cgccgccgac cccgacgcga acccgccggt tgccgccgaa
     1621 tacgaaaccg acgacgcatt ccgcgcccgc gtccaagccc atcccgaaaa atacgccgcc
     1681 gggccgcgca ccgcatacga agcccacgcc atcgacgcac acccccaaat cacacacgcc
     1741 cgcgccgtgc gccgcgccgt cggcacggtg gaggtttaca tcaaaaccca aagcggcacg
     1801 cccgacgaaa ccattttgac cgccgcgcgc gaatacctgc ccgccgaaac acgccgcccc
     1861 ctttgcgaca acgtgcaagt aaccgccgcc caacccaaag acgcggcagt agagtattcc
     1921 gccgaatacc accccgccgc caatattcaa gccgaacggc agaccgcctg cgaagccttg
     1981 gataacctat ggcggcaaaa cgcccacatc ggcgcatccg tcgccctgtc taaaatcatc
     2041 ggcgcgttag acacccccgg cgtgaaaaaa aatcacactg cacagccccg ctgccgacat
     2101 cgaatgcggc aacggcgaat acatccgaat cacgtccacg ctcgagcgcg aataaatgaa
     2161 cagcaccgtc cccgcgaaca acagccccct gcaacacgca ctggcaaagc tgacagaacg
     2221 cgaaactgcc gccgtctccc gccaactcga ccccgcccga tgcgaccccg gatttttacc
     2281 ctttcacgcc ttcgcaagaa gcatcggcac ggaagagggc tgggactttg ccgaaaccga
     2341 cgaagcccgc cgcaacctca tcgcaggctt tgccgaaatc cacgcccgaa aaggcacgcc
     2401 gtacgccatc cgcgccctct tccccatctt gcggctgggc gaaatccaaa ttatcgaacg
     2461 cgacggcgag ttcaagtggg acggctcggt cttgttcgac ggcagccgca cattcggcag
     2521 gcgcgagggt gactgggcgg aataccgcat tgtcttaacg cgccccgtca gcatccgcca
     2581 aaccgcccgc atccgcgcca tgttggcgga aatcgccccc ttgcggtgcg aacttaccgc
     2641 gctcgactac cgcaaccatc cccaccgctg gaacggcaaa atccgcttta acggcgaata
     2701 cggtttcggc acgacataac gcgcccccga aaatcaacaa acaaaaggaa gccccaaaat
     2761 ggcaaacgca accgaacaaa accaattcga ccaagccgtc cgcctcatcg aacccggcga
     2821 cagcgtcgtc gtcggcccag gcgcgcccgt caaccaacca ctgcaagccc tcgccaaccg
     2881 cacccttttg ctcaaaaacc aaaccgaagc ccttcagacg gcatccgaca ccaaagccgc
     2941 cgcaagcacc gccgtcaacg caggcgacgg actgacgggc ggcggcagcc ttgcccaaag
     3001 ccgcaccatc gcactgggcg cgcccgggca aatcaccgca acaagccaaa acaccgtccc
     3061 aaaaaacggc cacacccacg ccatcgacac cgcacgcacc gaccgcgccg gcatcgtccg
     3121 attagacaat gccattagcg aagccgaaga caccgccgcc acccccaaag ccgtcaaaac
     3181 cgcgctcgac caagcccgcg ccgccgccgc gaccgccgac ctcaaagtct ccctctccga
     3241 caaccaaacc gttaccggcc aaaaaacctt taccgccgaa acacaattcc aaagcggcat
     3301 ccggctgtcc gccaacccga cgcactgaaa cggcggctac aaagcctata tcggcgcgga
     3361 taacgaaaac gcccacatcg tcttcggcga cgacaccctg cgcctgcacg gcgcaaataa
     3421 ccgcatttcc tacaacaacc acgacatctt ccacaaagcc aacaaaccgc gtttcaacga
     3481 agacatcgaa ggcaaaccga acacgctgtc cggctacggc atcggcaact tcaaagtcga
     3541 aacattccgg ggcgatttga acagcctcaa aaccgacggc gtctattccc tgccgacggc
     3601 ggtcggcagc tcgaacctgc ccgttgaaaa caccgcctgc catatccaag tcatcgccgg
     3661 cacgcaaccc ggctggtgca ggcagttggg ctatcccgcc tacacgtccg acgtgtacga
     3721 acgctaccag accagcagcg caaacgacga ctggagcgcg tggaaaaaac tcaattcgga
     3781 aggcatcccc gccggcgcga tcgtgtcctt tcccaaagcc gtccgaaacc ccgcaggcta
     3841 tctcaaagcc gacggcacga tctttgcaca aaacaccttc tcgaccttta ccgcgccctg
     3901 ggcaacacaa acaaactgcc cgacctgacc cgtaccgaca tcggcatcac cgcgtggttt
     3961 ccgtccgacc aaatccagac gggctggctg gcgtttgacg acatccgaga gcgcgtaacc
     4021 gaatcggctt atcccgaact ttaccgcctg ctgaccgaaa aatacggacg catccaaaac
     4081 gtcccgcagg cggaagaccg ctttatccgc aacgcgggca acggcttggc ggtcggaacg
     4141 aagcaggaag acgaaatcaa acggcacgtc cacaaagtat tttcacactg ggcaaaccac
     4201 ccagacgccg ccgccgtcgg ttacgaagac cgcaatgaaa ggcaaagaag cgcgctcgta
     4261 tcgacttgga cggacggcga cttaaacgac aacggctttt taaccccgcg attggacagc
     4321 aaaatggcaa cgggcggcgc agaaaaccgc cccaaagccc tggttttaaa actgtgcatc
     4381 aaagccgccg acaccttggg cgaagccgtg ttccggataa agtcccacgg cgaaaccgcc
     4441 aacgccggcg cactggacgc gtcaaggctg gcgcaaggtt tgcaggaaaa agccgaccgc
     4501 gaccacaccc acaccaccgc ccaaatccaa gggctggacg aaaaaatcag cgccgccgtt
     4561 gccgcgcaat tcacacgcca aaccatcggc ggcgtggata ttgtcagatt ccccgacggc
     4621 acaatgatac agaccggcag ttacaggttt gcaagaggcg gcagccccat aggaaacgaa
     4681 gttgtcttcc ccatcgcctt tgccgacggc aacgtcaaat gcttcgtatc cgaacgccat
     4741 tcgggacgcg tcaacggcga aaggcaacac aactggctgt ttatccgcgc aaaaaaccac
     4801 gccgccgcca ttattaccaa ctggtacgaa agcagttgcg actggatggc catcggcaaa
     4861 tccgcctcgg gaaacgccgc cagccccacc ccgatagtcc ccgaaatacc tgaaatcgat
     4921 gaagaaccgc aaagagagag tggaagatca tcaaccggac tccgaaaccc ccgccgccac
     4981 cgaggcctcg acttccccgt ggggtcgtaa gactagcggg cggcagggca ggctgtagag
     5041 acgggctgta gagacgggct tcagcccacc gatccaagca atccgaccga aaccggccgc
     5101 gccgccaatc ctgcgaaacc tatgccccgc caatcctgcc actcttcgcc attcccgcga
     5161 aagcgggaat ccggaaccca aacgcggcag gaatctatcg gaaagaataa cccgaccgcc
     5221 gtcattcccg cgcagatgat atgttgcccg tcaacacaaa ataaaaaaca aagttgcaac
     5281 atgctgattt atattgttat ttttatttac gtttatttac gatatgcaaa tgcacggtta
     5341 cacaaatata ttcgcgtaac cgtttaattt tgttgaattt tattgattca atcggtgtct
     5401 ttccgcatcg taaggctggc cggttttaac aatgtaatag gcgggaatcc agacccacaa
     5461 cgttaaggcg gtttatcaga aaaaaccgaa accgaacgcc ctagatttcc gcctgcgcgg
     5521 gaatgacgaa gagttgggga gtgacgaaag gcgggaacaa ccgcgcaaaa agccgccgac
     5581 tccttcagac ggcatcggca ataaaaaccg cacggccgaa accgcgcggg aaaggatagc
     5641 cgggcgcgcc cgataagcag cggccgcagg gttaagtcct tgcacctgtt ttcatcgaag
     5701 acaaaggcgg ttcgggaaat aagggcgcaa tatcaaaagt tttgcgttgc ctgatttgcc
     5761 acaaatcata accggcctgc atattcagcc aaaaatcagg cgaagtgttg agcagttggc
     5821 ttaaacgaac cgccatctcg ggcgtaatcg aagattgggc gttgacaatg cgcgataagg
     5881 caacccggct gacacccagg cgtttcgcta tatcggtaat gcttgcgccg ttaatatact
     5941 cttttaaaac aaggcccggg tgggcaggat tgtgcatttt catattattc ctagtgataa
     6001 tcctgataat cgacaacttc gacattgccg tcgttgaaac gaaaagtcaa acgccaattt
     6061 ccgttgactt tgaaccgccc aatgtcccga caaactgccc ttgaggggat gaagattcca
     6121 actcggcaca gccatatccg aaggcgtttg cgcggcattc agcgcggtta gaagcagatt
     6181 cagtttgacg gaatgcccgg cttggatgcc cgatagcgag cctgttttaa aaaagcgttc
     6241 aagccctttg tgtttgaaag aaacaatcat catcccaccc cgtctgtatc gccaaaggat
     6301 acaaaacttt tatttgattg taaagcgacg gtatacaaaa aaggaaaccc cgaaatgacc
     6361 atctatttca aaaacggctt ttacgacgac acattgggca gcgtccccga aggcgcggtt
     6421 gccgtcggcg gcgaagaata cgccgccctc ttcgcaggac aggcgcaggg cgggcaaatt
     6481 gccgccgatt ccgacggccg ccccgtttta accccgccgc gcccgtccga gtaccacgaa
     6541 tgggacggca aaaaatggaa aatcagcaaa gccgccgccc gtttcgccaa ctcccgtttc
     6601 gccaaacaaa aaaccgcctt ggcattcagc ctcgcggaaa aggcggacga actcaaaaca
     6661 gcctcttggc gggctatccc caagtggaaa tcgacagctt ttacaggcag gaaaaagaaa
     6721 ccctcgcgtg gcaggcggac aacgacgccc cgaccccgat gctggcgcaa atcgccgccg
     6781 caaggggcat agaattggac gttttgattg gaaaggttgt cgaaaaaccc gcccgcctgg
     6841 ccgttgccgt cgacgcgatt atcggaaagc ggcagcaact tgaagataga ttaaatacca
     6901 tcgaaaccgc gccagcatta gatgcgttag aaagggaaat caaagaatgg gcgctaaacg
     6961 tcggctgaga gaatatattt atcatctact agtagccata gaccaactgt tcaacgctat
     7021 aaccggcggc gcggcagacg aaaccctctc aagtcgcacc tatcgcggcg cgcggctcgc
     7081 ccaacaaccc aaaacccgct ggaagatttt atagtggatt aaatttaaat caggacaagg
     7141 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggtaacgccg taccggtttt
     7201 tgttaatcca ctatatatac cctgatcaac ggcgtatttt tcgaccgcca ccactgccgg
     7261 caggcgtata tcagcgaact gaaaggcagg cagcacgacg cgcggttcaa ccaaagccgc
     7321 gccgccaatg aaagggatac gcggtaaaat tgccgccgcg ccgcattcag acggcatcgg
     7381 caataaaaaa gcccgaaaaa atcgggctga aagttaaggc tgcatcgcct ttaaggcggc
     7441 tttcgccaaa aaaccgctcg ggtttcatga cgggcggcag tgcaggcatc gacacggttg
     7501 aggatatgtt gcggccaact gacgttaaac cgcacctgtc cggttaatgc ggcatcgtcc
     7561 gcttcaacca tcgtccacaa caccgcatcg gcataatcgg gattgtcccg attaacctct
     7621 atgctttgcg cctcgggaat cggcaggttt tcctgcaaaa gcccttcgag atggaacatt
     7681 gcggcggctt tggcatcggc aagcgcgtcg gcaaccgtat cgccgacagg atgcagcccg
     7741 gcagatcggg aataatcacg ccccagttgc tttgatcggg gttttggaac agcacggcgg
     7801 gatagtaaat cattgcttcc tttctcggat atgcggtatc ggcaaagtcc aaacgcaacg
     7861 aaggggattt aaaaacgaca aaaacgcaaa agccgccgac attcccgcat ccaagtttca
     7921 gtcaatcaga taaccttgga tttctttggt tttcgcattg atttctctgg tacggcagtc
     7981 agattgtgcc accccgtatt cgtcgccgtc ggcgcatttg gcatccaaac cgttttgcca
     8041 gttgcggtac tcgggcataa cggtttcggc aatatcggcg ggcagtgcct gaaccgcgct
     8101 gttcagagcc gctttggcag ttctttcttg tcctcttccc tgaccatttc gtcgataagg
     8161 gcttttttac ggttgtgcag ctcttccaag cgtgcttcgg tttctgccgt tttacacgcc
     8221 aactcactga cttttatgcc gtttggcgtt tcaccttcgg ctttcgcatt gttggcacag
     8281 atcttgtcca taccgctttt ccatgttttc tgcgaggctt gcagcttatt ctgtacagtt
     8341 tgaggcaatc ctttccaaaa ctgctcgaac tcgctgcgcg acagcttgta gcgttcttcc
     8401 cattcggata cgcgggcggc ttcctgctcc cgacccaatg cctcctgcgc cgccgcttct
     8461 tctgcctccc tcgcttcctg cgcggtggct tctgccgctt caagtttttc gttcctttgt
     8521 ttcgctttgt ccaacggctc tttaatcagt gccatagaca ccagctcgct gagcggatgg
     8581 atgatatcgt gtgcctgact gagttcggca aagattttgc ttttgtcgtc tgtcggctgg
     8641 acgctgtaag aaatagtttt gacataatac gcgccttctt tgcggtagta gggttcaaaa
     8701 aagtcgggcg ttttcttatg gctgttgcct atagattggt tggcggcgac ggcataatcg
     8761 acaacatcgt cgggcacgtc cagttttaac gcggcttcac atttgagttt gctggacgtg
     8821 tcggttttct cggtagttcg gacttcatcg acggttatgc ccaaacgttc gaccattctg
     8881 cggatatgcg atatgccgat atcggcaaat gcttggtctt tgaatgcgtc gtcgtcaaaa
     8941 gttttgacgg tttcaccttc gacattgtcg cggaccagtt tgaccaaaat ctctttagtc
     9001 tcttcgcttc cgcaggaaat ttcctttccg gaaaacgggt tgcctattcc caaacggtcg
     9061 cagcccgaca aaatcagcat acttgccaat gccagtgcgg gtaaattttt tttcattaga
     9121 tttcctttgc cgtccaaaca cggactgtct gttgcgtatt tccacacacg ctacggcact
     9181 tgaagtacat ggctgcggaa catatatgtt taaatttatg caaaccgcca tatcgcgatt
     9241 gcacacgccc tgcaaattta ccgacggttt tcctgttcga taaaaatgcc gtctgaaacg
     9301 gtcggcgttc agacggcatt tttccgcagg ttttatttgc ggttggtctg caggtagagg
     9361 ttaatcaggc gttcggtcga gctgtcgtgt ttttgaggtt cggtttcgcc tgtcagttcg
     9421 cccaaaatgg ttttagccag ttgtttgccg agttccacgc cccactggtc gaagctgttg
     9481 atgccccaaa tgatgccttg gacgaaggtt ttgtgttcgt acatggcaat caggctgccc
     9541 atattgcggg ggttgacctt atccatcagg atgaggttgg tcgggcggtt gccggagaag
     9601 gttttgtgcg gaaccagctc ttcgatgcgc gcctcatcca taccctgcgc tttgagttcg
     9661 gcgcggactt cgtcgggggt tttgccgcac ataaaggctt ctgcttgtgc aaagacgttg
     9721 gcaagcagga tttcgtggtg tccgggcagg ttgctgcgtt tttcaagcga ggcaatcagg
     9781 tcgatggggg tgatgtgcgt gccttgatgc agcagttgga agaaggcgtg ctgaccgtta
     9841 atgcccgttt ctccccagat aatcggcgag gtttcgtgtc cgactgcttt gccgtccaac
     9901 gtaacctgtt tgccgttgct ttccatatcg agctgctgga tgaatttggg caggcggtgc
     9961 aaatgttggt cgtaaggcgc gatgacgtgg ctgccgccac cgtagtagtt gatataccag
    10021 atgccgatga gggcgaggat gacgggcagg ttgcgctcga gcggggtgtt gatgaagtgt
    10081 tggtccatca ggtgcgcgcc gttgagcatt tcgatgaagt tttcttcgcc gagatacagc
    10141 ataatcggca atccgatggc ggaccacagg ctgtaccgac cgccgaccca atcccaaaat
    10201 tcaaacatat tggcgatgtc gatgccgaat tcggcgacgg cttttcgatt ggtggaaacg
    10261 gcggcgaagt gtttggcaac ggcttcttcg tcacccgcat gattcaaaaa ccattcgcgc
    10321 gcggttagcg cgttggtcag cgtttcctgc gtggtaaatg ttttggaggc gatgatgaac
    10381 agcgtggttt ccgggtggac tttggacaat acgtcgcgca gttgcgagcc gtccacgttg
    10441 gagacgaagt gcatattgag gcgcggatga ccgaaaggtt tgagcgcggt acacatcgtc
    10501 agcgggccca aatccgatcc gccgatgccg atgttgacaa cgtcggtaat gacttggttg
    10561 gtatagccca gccagcttcc gctgcggact tcgtgtgcaa attcgcccat acgttgcaaa
    10621 acgcggttga ctttgggcat cacatcttca ccgtcaacca taatcggcga attggtgcgg
    10681 ttgcgaaggg cgacatgcag gacggcgcgg ttttcggtgg tattgatttt ttcgccgtgg
    10741 aacatctgcc gcatccgctc cggcacgcct gcttcacgcg ccaactcgaa cagtagggac
    10801 atggtttcgt cgttgatgcg gtttttggag tagtccagcg tcagtccgcc gacttgcagc
    10861 cagtagcgtt ccgcccgctg cgggtcttgc tcgaacattt cgcgcatatg caatgttttg
    10921 ctgtcgtcaa agtggttcca caattttgac caggccggta agtcgtgaag gtgtttcatc
    10981 tgtatgctcc cgattgaggt tttccgtgac ggggggcgtt tccgcacatc gatgcgcaca
    11041 taaggcgcaa ccctgcgtac cgccccgatt tggaaggggt tgcggcaaac ccgaaccctg
    11101 ccgccttaaa gggcggggtt tccgccatta aggaaattct gataaaaggc ttgccggagc
    11161 ctgtccggcc gttcagacgg cattggcggg atgccgtctg aacatcagtc tttgtcgtaa
    11221 tcaatgtgct tgttgtgtat gctttttttg cttttctgca attgcaggct ggcagcatcg
    11281 cccaagcgca gggcaagtcc gatggcgaga atatcgatga cggcaagctg caagaggcgg
    11341 gaaaccatgg gcgtgtagag ttcggcgttt tcctgtgtgg caacgctcaa cacgcagtcg
    11401 gcaagttgcg ccagaggcga atcgttgcgg gtcagtgcga tgacggacgc gccgttttct
    11461 ttggcgatgc tgaccgcatc caaaagttcg atagacgaac ccgtgttgga aatggcaacc
    11521 aaaacatcct gatcgctcaa aacggatgcc gccatcagtt gcgtgtgcgt atcgacatag
    11581 gcgacggtgg acataccgaa acggaaaaat ttgtgctgcg cgtcctgtgc cacaatgccg
    11641 gaattgccca cgccgtaaaa ttcgacgcgg cgggcgtgca tcagggtggc gatgccgttt
    11701 tcgagttcgg attcttttaa gaacctcctc tcccccaaca aggctgcggc ggcgttgccc
    11761 aacacttttt caaccacatt cgccatatcg tcgtcggcat tgagttcttc gtggacataa
    11821 ggcatacctt cgtgcccgat gctggcggac agggagagct tgaattccgg cagcccctta
    11881 taaccgaggc tgcggcagaa ccggatgacg gtcggctggc tgaccgaggc gcggtcggcg
    11941 atttcggcga cggcggcgtg gacaaaccac ttgggttctg ccaaggcgca ttcggcaact
    12001 ttgcgttccg caccggacag gtcggccagt gattcgctga ttttgcttaa cataatgata
    12061 tgcccttcga taatgcggcc ctgctgcaag ggctgccgtg gttagacgtt tttcaggtgg
    12121 ttgccgagcg ccgccgccgc gccggcaata cccggaaatt cgctcaagac gacataaacg
    12181 ggaatcgcgg caaggtaggc ttcaaaacgc cctttgtttt caaagcggct gcggaagggg
    12241 gaggttttga agtaatccaa cattcgcggg ataatgccgc cgcacaaata cacgcccccg
    12301 cgcgcgccca aagtcagggc gaggttggaa gcgaccgtgc ccagcatcgc gcagaaaatg
    12361 tccaatgcct gccggcacag gggcgattcg caatttaatg ccttttcggt gatttcagac
    12421 ggcatcagtt ttgccggttc ggctttctgc ttggccgcca gcgtttcata aatcaggctc
    12481 aaacctgcgc cgctgaggaa acgttcggcg gaaacgtggc ggtatttgtt tttggcgtac
    12541 tgccaaatca acacttccat atcgtcaaac ggcgggaaac tggtatgccc gccctcgccc
    12601 gccaacgcca cccagcctgc ggggctgtgc accaatccgc tcacgcccaa gccggtaccg
    12661 gggccgataa cggccttggg ggcgaattcg acaggctttt gcccgcctac ctgcatcagg
    12721 tctttgcttg aagtctgcgt taccgccaat gcctgcgcgg taaagtcgtt caaaaggatg
    12781 agggtgtcca gccccaaagc ctgacgggtg gtttcgatgg aaaacgccca atggtggttg
    12841 gtcatctgca cccagtcgcc caaaatcggg ttggcgatgg caaatgccgc gtgccgtacg
    12901 cccgttgcac cactttggtt cagataggca cgcaccgcgt cggtaaccgt gtcgtattcc
    12961 ttacacggca ggacggcgac tttctcaatc acgcacggcg cggtttccaa tgcgaaacgg
    13021 gcgtttgtgc cgccgatgtc ggcaaccagc ctcgggtaat cggcgtgttt attcggcgta
    13081 gaagacatgg cagttcactc cttgatggtt caaaacgagg ctgatcggat attcgcggtt
    13141 ttcgccttgt gcggcttggt cgaacacggc ttttttctct tcgccctgta tcgccaaaaa
    13201 tacatgccca gtatgggcaa tcgcatccaa agtcatgctg atgcgctcgt gcggcgcggt
    13261 aacgggcgtg gtatgcacca gcgcgacacc tgccgaaccg tcgattgccg tctgaaactg
    13321 cggagctttt gggaaaatcg aagccgtatg cccgtcgttt cccataccca aaatcaaaac
    13381 atcgggctgc ttgtaatgtt tcagcgcata atcgacaaca gcatcgggat gtaattcggt
    13441 ttcagttttt ccgtcttcca ccataggaat ccacactgcc gcagcggctt tgttctttaa
    13501 caggtattcg cgcaccagac cggtattgct gtcggcgtgg ttggtcggca cgatgcgttc
    13561 atctgccaag gtaattccga cgtttttcca atccaaatct ttttgcgaca gggcgttgaa
    13621 aaatgcaatc ggcgaacgtc cgccggaaac tgccaacacc gcaccgccct tctcgtccag
    13681 tgcgccctgc aaagcatccg ccactgcgtc agccaaagac tgcgccgctt ctgccgcatt
    13741 ttcgtattcg tgccaaacaa acatatttgt gtccttttgt gatttcagac ggcatatccc
    13801 gttacagggc ggctgccggg caatggctta gccgcttccg cctcgaagcg ggactgcttt
    13861 ttctgcccgc cctccttcag acggcattgc gaacccgatg ccgtctgaag cgcattactg
    13921 ttcttcgtgc cacttgttgc cgtcgcgcgc caacagttcg cgggcggctt cagggcccca
    13981 agagtgtgcg ccgtaaccgt gcggcggcgt ggtgttattt gcccagtttt ccaaaatcgg
    14041 catcacatat tcccacgcgg cttcgagttc gtcgcggcgg ttgaacaaag cgagtttgcc
    14101 gttaatcaca tccaacagca ggcgttcgta agcctcggcg cggcggcctt caacggcctt
    14161 gcccaaatcc acgcccaaag gcgttacttc cactttgttg cccgcacccg gggttttcac
    14221 ctgcgtgtaa aggcggacgg attcattcgg ttgaagctcg ataaccaaac ggttcggcgc
    14281 ggtttggctg ttgtcgaaaa tatggttttg cagcgggcgg aagttcaaaa cgatttccgc
    14341 cactttgccc gccatgcgtt tgccggtacg caggtagaag ggaacgccct tccagcgttc
    14401 gttttcgatt tcggctttaa tggcgacgta ggtttcggta aagctgtctt gcggaacgtt
    14461 gatttcttca agatagccgt tcatgccttt ggcagcggta tattgtccgc gcacgacatt
    14521 ttcattgaca gattcgatgg tcagcggctt caatgacttg atgactttga ctttttcatc
    14581 gcgcaccgcg tcggcatcca agctggcggg ggcttccatc gcagtcatgc acagcatctg
    14641 catcaaatgg ttttgcacca tatcgcgcaa cgcgccggta atgtcgtaaa actcgccgcg
    14701 ctcttccaca ccgagctgtt cggcgatggt cagctgcacg ctttcgatgt atttattgtt
    14761 ccacagcggc tcgaacatta cattggcaaa acgcagcgca agcaggtttt gcaggctttc
    14821 tttgccaagg tagtggtcga tgcggtaaat ttgcccttct ttgaaataac gcgccacatc
    14881 ggtattgatt tgtttggaag aagccaaatc cgtacccagc ggtttttcca aaaccacgcg
    14941 cacattgtcg gcattcaaac cgatcgcagc aaggttttcg caggcttgcg cgaagaattt
    15001 cggcgcggtg gacagataga tgacgacgtt gtcggtttct ttgcgtgctt tgaccaaatc
    15061 gcccaaagcg gcaaaatcgt ccggctgcgt aacatcgact ttgagatatg cgagacgttc
    15121 aacaaacgaa gcccacgcct caccggaaaa attttctttc acatggattt tggaactggt
    15181 ttccaccttc gccagaaaac cttcggtatc caactcgctg cggctgaccc ccaaaatacg
    15241 cccttcggga tgaagcagac cggcaacatg cgcctgatac aggcagggca aaagtttgcg
    15301 cattgccaaa tcgccggtcg caccgaacaa caccaaatca aaatttgtct gtgtactcat
    15361 cgcattatct cgtcaggaaa ggattttttc gatgccgtct gaaacctgtt tttttccatc
    15421 acgcagcatc gcaatatcgg aaacaaaggc aggcggcata atgagtagta atactacaca
    15481 ccgctacact ttttgtctat tcccattttt acaatttatt tgacctagtc caaaaatcag
    15541 gcaggtttcc cctattccgt tacaacaatc agaagattct gcgatttaaa tcaaatttct
    15601 tttcaatgtc tgattttttt gtaacaaaat tacaaatttt gtactataat agcacccgct
    15661 tcccactttc agacggcgta ccttttaaaa tatagtgaat taaatttaaa tcaggacaag
    15721 gcggcgagcc gcagacagca caaatagtac ggcaaggcga ggcaacgccg gaccggttta
    15781 aatttaatcc actatactca ccgtctaaca tacccgatac aaaaatcaga aacgcacaaa
    15841 caaatcccca ataccacccc gttccgccag gagaccgaac atgaaccaca ctcctatcca
    15901 ccccaaactc gccgaaatca ccgggcgcat tatcgaacgc agccgtccga cgcgtgaaaa
    15961 atatctggcg aaaatccgca gtgccaaaca aatgggacgc ttagagcgca accagctcgg
    16021 ctgcagcaat ttggcgcacg gctatgccgc catgcccaaa agcatcaaaa tcgaaatgct
    16081 tcaggaaacc gtccccaact taggcatcat cactgcctac aacgacatgg tttccgcaca
    16141 ccagccgttt aaagacttcc ctgaccaaat caaagacgaa gcacaaaaaa acggcgcgac
    16201 cgcacaagtc gccggcggca cgcctgccat gtgcgacggc atcacgcaag gctacgccgg
    16261 catggaattg tcgctgtttt cccgcgacgt gattgcgatg agtaccgccg tcgggctgtc
    16321 gcaccaaatg tttgacggca gcctgtttat gggcgtgtgc gacaaaatcg ttccgggtct
    16381 gatgataggt gcgctttcgt tcggtcatat tcccggtatc ttcgttccgg caggcccgat
    16441 gtccagcggc atcggcaata aagaaaaagc ccgtacccgc caacttttcg ccgaaggcaa
    16501 agtcggacgc gacgaacttt tgaaaagcga aatgggttct taccacagcc cgggcacctg
    16561 cactttctac ggcacggcaa actccaacca aatgatgatg gaaatgatgg gcgtacacct
    16621 gcctgccgcc gcctttgtcc acccctatac cgacctgcgc gaagccctga cccgctacgc
    16681 cgccggacac ctcgcgcgcg gcatcaaaaa cggcacgatt aaacctttgg gcgaaatgtt
    16741 gaccgaaaaa tccttcatca atgccctgat cggtttgatg gcgaccggcg gctcgaccaa
    16801 ccacaccatg cacctcgtcg ctatggcgcg cgccgcaggc gtgattttga actgggacga
    16861 cttcgacgaa atttcttcca tcatcccgct gctcatccgc gtgtatccca acggcaaagc
    16921 cgacgtgaac cactttaccg cagcaggcgg cctgcctttc gttatccgcg aattgctgga
    16981 cgcaggctta ttgcacgacg atgtcgatac cgtcgtcgga cacggtatgc gccactacac
    17041 taaagagcct ttcctcatcg acggcaaact cgaatggcgc gaagcccccg agaccagcgg
    17101 caacgacgac atcctgcgta aagccgacaa tccgttctcc ccggacggcg gtctgcgcct
    17161 gatgaaaggc aacatcggac gcggcgtggt taaagtgtca gccgtacgcg aaggctgccg
    17221 catcatcgaa gcacccgcca tcgtgttcaa cgaccagcgc gaagtgttgg cggcatttga
    17281 acgcggcgag ttggaacgcg attttatctg cgtcgtccgc taccaaggcc cgcgtgccaa
    17341 cggtatgccc gaattgcaca aactgacccc gcctttgggc atcctgcaag accgcggctt
    17401 caaagtggcg ctgctgaccg acggccgtat gtccggcgcg tcaggcaaag tgcccgcgtc
    17461 catccacatg acgcccgaag ccctgatggg cggcaacatc gccaaaatcc gtaccggcga
    17521 cctgattcgc ttcgactccg ttaccggcga actcaacgtc ctgattaacg aggccgaatg
    17581 gaacgtccga gaagtcgagc gcatcgattt gggcgcgaac caacaaggct gcggccgcga
    17641 actcttcgcc ggcttccgca gtatgaccag cagtgcggaa accggtgcga tgagcttcgg
    17701 cggagaattt gcctgatgcg cgtttcagac ggccttttaa aacagaaggc cgtctgaaaa
    17761 attattgaag ccgtttaaaa tagatgcaga cgtagcgtga gctttgcccg cgaacaaaac
    17821 ccaagcaaca tcagaatgtt ttaccccaaa ggttgtctga aaacttaacc cccccatccc
    17881 gtttgacctc aacaaaaacg acacccccca cttggagaac cgaaatgtcc aaactgaccc
    17941 cccgcgaaat cctgaccgcc ggcgcagttg tgcctgtgat ggcgattgac gacttaagca
    18001 ccgccgtcga tttgtcccgc gcccttgtcg aaggcggcat ccccaccctc gaaattacct
    18061 tgcgcacccc cgtcggactc gaggccatcc gcctgattgc caaagaagtg cccaacgcca
    18121 tcgtcggcgc aggtacggta accaatcccg aacagctcaa agccgtcgaa gacgcaggcg
    18181 cggttttcgc catcagcccg ggtttgcacg aatccctcgc cagagccggc cgcaacagcg
    18241 gcattcccct gattcccggc gttgccactc cgggcgaagt ccaactggct ttggaacacg
    18301 gcatcgacac actcaaactc ttccctgccg aagtcgttgg cggcaaagcg atgctcaaag
    18361 ccctctacgg accttacgcc gacgtacgct tctgcccgac cggcggcatc agcctcgcca
    18421 ctgcgcccga ttacttggca ttgcccaacg tcttatgcgt cggcggctct tggctgacac
    18481 cgaaagaagc tgtaaaaaac aaagactggg acaccatcac ccgcctcgcc aaagaagcgg
    18541 cggcattgaa acccaaagcc tgaaccgcat cgtaaaaatg ccgtctgaag ccctttccag
    18601 tctcagacgg cattttgccg cttggcgact cagtcggcat acacagcagc accgccccta
    18661 aaatgcccat ccatctccag aacaatgcga atgacgcgcg atatgcggag acctatgacg
    18721 aaaaaataat tataatatat ttatcaatta gtcataaaaa tcgccctatc ggtaaaatgc
    18781 cgtgatgagc ggctgaaact aacaagttaa ccgtcgttaa aaaataacgc tcagagacct
    18841 ttgcaaaatt ccttttcttc aaacagccga catccaacca caggtttttc ggctgttttc
    18901 gtttcaaata accgccgttc ccaccaaaat atcccattaa tcccccttaa atatagtgga
    18961 ttaaatttaa accagtacgg cgttacctcg ccttgccgta ctattttact gtctgcggct
    19021 tcgttgcctt gtcctgattt aaatttaatc cactatacct gattgatcag gaacagcccg
    19081 ttttacacgg ctgaaaaaat gccgtctgaa acacgttcag acggcatccg cttaaaaaac
    19141 aactgattca acggcgatta atccgcttcc aaaaccactt tcatcgcttg gttttcggcg
    19201 gcgtgtttga acacgtcgta ggctttttcc aattcgctga atttgaaacg gtgggtcaac
    19261 attttggtgt aatccactga gctgctgaaa attgccttca tcagtatttc ggtggtgttg
    19321 gcattgacca aaccggtagt gatggcaaga tttttaatcc acagtttttc cagtttgaaa
    19381 tcaacggatt gaccgtgtac gccgacgacg gcgatatgac cgccgggttt cacgatgtct
    19441 tggcacatat tccaagttgc agggataccg accgcttcga tggcacaatc tacgccgtct
    19501 tcgccgacga tggcgaaaac ttgtttggag acgtcgccgg aagcggggct gatggtatgg
    19561 gtcgcgccca actctttcgc cggtttcaaa cggttttcgt ccatatcgca cacgataatg
    19621 gcggcagggc tatacagttg ggcggtcaac aaggcggaca taccgacagg gccggcacct
    19681 gcgatgaata cggtatcgcc gggtttcaca tcgccgtatt gcacgccgat ttcgtgggcg
    19741 gtcggtaaag cgtcgctcaa cagcagggcg atttcttcgt tgacgttgtc gtgcggcggc
    19801 acgaggctgt tgtcggcata aggcgtgcgg acgtattcgg cctgcgtgcc gtcaatcata
    19861 tagcccaaaa tccagccgcc gttgcggcag tgcgaataaa gttggatttt gcagttgtcg
    19921 caagtgcagc atttgctgac gcatgaaata atgactttat cgccgacttt aatgtttttt
    19981 acagcctcgc cgacttcttc tacaataccg atgccttcat gaccgaggat acgaccgtcg
    20041 gcgacttcgg ggtttttgcc tttccaaata cccaagtcgg taccgcaaat cgtggttttg
    20101 acgattttca ccaccgcatc ggtcggatcg ataatctgcg ggcggggttt ttcttcaaaa
    20161 cggatgtcgt ttgcgccgtg ataaaccatt gctttcatgc tgatactcct tgcttgttga
    20221 taaataattt caataccaca ataaagtttc tttatatgag ttatatgccc ctacaaaaaa
    20281 taagtcaata agaattattt tcacaatttt atacaacaac atactgtttt aaatataaat
    20341 aaaaccaccg attgatatta atgaacacac ccatcccctt ctccgaacgg ctcatccgct
    20401 ggcaaaaaca acacggtcgc caccacctcc cttggcaggt caaaaaccct tattgcgtct
    20461 ggctttccga aatcatgctc cagcaaacgc aggtcgccgc cgtgttggac tactatccgc
    20521 gtttcttgga aaaattcccg accgttcaga cgcttgccgc cgcgccgcaa gacgaagtgt
    20581 tgtcgttgtg ggcgggcttg ggctattacg gccgcgcgcg caatctgcac aaagccgcgc
    20641 aacaaatcgt cggacaattc ggcggtacgt ttccatcgga gcgcaaagac ttggaaacgc
    20701 tctgcggcgt aggcagaagc accgccgccg ccatttctgc ctttgctttc aaccgacgag
    20761 aaaccatctt ggacggcaac gtcaaacgcg ttctctgccg tgtttttgcc caagacggca
    20821 atccgcaaga taaaaaattt gaaaactcgc tctggacact tgccgaaagc ctgatgccgt
    20881 ctgaaaacgc cgatatgcct acttacacgc aaggtttgat ggatttgggc gcgaccgtgt
    20941 gcaaacggac gaaacccttg tgccgtcaat gccctatggc ggacatctgc gaagcaaaaa
    21001 agcaaaaccg caccgccgag ctgccgcgta aaaaaactgc cctcgaagtg caaaccctgc
    21061 cgctttactg gctgattgtc cgcaaccggg acggcgcaat cttgctggaa aaacgcaccg
    21121 ccaaaggcat ttggggcgga ctgtattgcg tgccgtgttt tgaaagtttg aacgggcttt
    21181 ccgactttgc cgccaaactc tccctgacga tggcggatat ggacgaacaa accgccctga
    21241 cccaccgcct gacgcaccgg ctgctgatga ttacgccgtt tgaagggcaa atgccgtccg
    21301 aacaccattc agacggcatt tggataaagc cgggccattt gaaagattac ggcctgccca
    21361 agcctttgga aatttattta aacggtaata ggttagaata aacaaaataa acccgttgaa
    21421 ctgttgttag caggtatcgc agcaagaaca accgatgaat ttgggtcgta ttttaggcag
    21481 cgggataatg ttcaaatggg acatttggaa cggaaaaagt cggcaattta aaaaggattt
    21541 tagaaacaaa gagggtcaaa aacatgaaca caaacttaaa tgacaaagac aaagccatgg
    21601 ataccgcaat caggtttcag aaaaggatga ggattccgaa atttttcttt ttaattctcg
    21661 gaatcacagt ggttttggca tttatccaag acgtgataac gggttctaat tttctgcaaa
    21721 taacaattat gtaaataata tttgtgatat atttcagttt cggaggtatc ataattttat
    21781 tctacataat catcgcagga atctgcccct attgccacga attccaaaag atgaattatg
    21841 gcacctccgt cggcatcggg tcggatagct ttactttctt taaaggcatt tcccctgttc
    21901 caaaaatcga ttactgcagc aaatgcggta agcccctgtc tccagaagcc gtaaataagg
    21961 tgatgaatga taaaaccgat gaagaaatgt gaacagcgtt ttaacaacag tgaaataaaa
    22021 tataaaattt caattttaaa ataatgagct aatggaaatt tgacccggtt gtccggtaca
    22081 gccccaaaca taaaacaaaa gctgcctgca aaaaaccggc agcttttgtg tatcttgtca
    22141 tacattattc acttcaaccg catcaaaacg aataacccac ctgaaacccc gttacccatt
    22201 tcttcgtctg aaaatattcg ggctttttca atgcacggcc ggtaaatata tcgtaatgca
    22261 ggttgccgcc aagctttatc tgcccgcgta tcccaattgc tgtgccggct agagtttggc
    22321 ccgataacca tttggcggat tgtcctgaaa catgtcctac atcagcccca agataaagct
    22381 gatggcctgg tttaaattgc cagctcaaat cgttgcgcca ataccatccc cgctcggcag
    22441 gcaaactcat ttcaccgtcg aagccacgta cggtgtggtg tccgccgata gccagtttgt
    22501 cttgcgatgt tagcggggtt ttgttccatt gtgcatgaac ggatgtgtca taggcaaata
    22561 gctgtttacc gatttgaaaa ggagtattta catcagccga tgccgtccaa attttcatac
    22621 gtgacgtgcc ttcgccaaag gcttcttcag gcgcgcgcag agcatctttc atgccggtgc
    22681 cgtgtttata tttcaactta aaatctgccg tactgcgacc gatatatcct ttgtgggaaa
    22741 gttctgccaa ccaacctgtg gttttacgcc gttgtacagt cagttcggca tcatcaatgt
    22801 aactttttgt ttccctcgtc cacagtttta cactgagata ggttttgcgt ttggcatcac
    22861 gatacaacag gcggttgaag ccgaaatcag tgttgtaact ttttccatta tagtcataga
    22921 cttccgataa tccggaaacc gcctgatggt aacggtagcc attgtgattg aatgcccatg
    22981 tccatttacc gaaaggggct gaataatgta cggcgtaatt gtttgatccg ccttctttgc
    23041 gatggccgtc aaaattttcc tcatcgggcg taccgccaat tgaacgtcca taatttacat
    23101 agaacatatc actcagtcca aaaggattgt cggcagagaa agtgatattt ccttggtatt
    23161 ttcctgtcgc ctcactaccc gaattatcca tccccacact cacacagtag ggcagcagac
    23221 gttaccgcca ttgcaccacg acatcacttt ggtttggttc tctctctacg ggaacgattt
    23281 ggagatcggc ttccgcagtc gggagacatt tgagattttc cagtccttgt tccaaatcac
    23341 gcagattcaa cagatcgttc gagcgggtgg gaaatttgtt ttggaatgct gcaatacgtc
    23401 ctgcatgggt ttgatcatcg ttggaccgat cgattcgtat ggagcgcaga tagcccggca
    23461 tcagggttaa ttgaagcttg ccactattca aatcctgtgg cgcagccaag atacgggtcg
    23521 tggtatatcc cctgccgatc aaagcatttt gtgctaagga catgatttga ttaatgtcgc
    23581 ccgcatgcag acacttgccg gaaacaaaat gtgtttggca caaggcatgg ttgagcgcaa
    23641 accggaattt agccgcttct tcacccacca attccacctc agaaatagga aagcaggggc
    23701 ttgcctcatc cgaatttgca ccgcctacct gtgttgccat cttctctatg cccgtatcgg
    23761 taccatccaa acgaacatct tgttccggct gcatctgttc acgcaattgc ttctcacgtt
    23821 gacgttggag aatatcctgt tgttgtgaaa cagcttcagg tgtgggggcg gctgatgtta
    23881 gggtggggag gcagccatca atgataggac taacagtgat aagggatagg gatgaaatac
    23941 atttttggag gaaaagaaag gtttcatagg attggtctaa gtaatgtaag cgggcaatta
    24001 tagcaatgca caaaaacaaa caaaaagata aaaaaactcc ataaatgaaa atttaatcac
    24061 atgtttatag gatatgaaaa agaggaagcg aaatctgaag ccgtctgaaa acaaccccac
    24121 acagcatcgg cggacgagtt tccctcagct gctccggcag ccgtacccag catccttgta
    24181 atcgccgaaa cggtctcttt ttcctccgca ttcaggctgc cgccgtctcc tttgccgtac
    24241 agccatttgc cgatgattgg ggcggccgct tccgacccgc ccgcacccaa tgctcctgcc
    24301 ggggcattgt tgcctcatgc tgcggcaacc gctgctccta ataccgcgtg ggcaagaacg
    24361 tgagcggttt cttgactggc ggttagtttg ccattcgcgt tttgaccggc taaatcttta
    24421 aagtgctgtc caatcgcata cgatacgtct ggcgatgcgg tagccgcagc gatgcccgct
    24481 ccgctttggg tcggctcagc taaacctgag gctaacatgt tgagaatgac tttgccttgt
    24541 tgccaattat ctgcttttgc tgccgcatct tgagcttcat gggctttgcg tttggcagtt
    24601 tccatatcgc cattggctaa tgcctcggct gctgctgttt cggctgcttc tttgtctgct
    24661 ttgagtttgt ctaaatgttg gttaatctcg gtattggttt gttgaacatt tttactaaaa
    24721 tcttggctga cggttctttg taaatccagt tcactttgca ctctatcttt gtcaaatatg
    24781 tttttcaggc tgcccgaatg tcgttcggcg gtgtctgtgg ttacgtttgt atcaatatcg
    24841 gctttggttt gtgccgctat tttgcctgtc agcctgattt gtgcggcttc gtctgtgatt
    24901 tgaatgtttt tgggtattga tgccgctttt tgtgatgctg ctttgactgt cgctgtcgct
    24961 gccgtaccct actgatgaac ttgcgccgtt tttatcggct atgcttgtca ggtgtttgtc
    25021 ttgaggttta ttttttgcgc cctgtcccag tgttttgccg cttacggcaa cgctggcacc
    25081 caatccaaaa ctttttcctt cgtattggct gtaattttga atatcactgc cggcgagtgt
    25141 gcctgtgctg aatcggtttt tacccttgtc ttttgcgctt tggctgctgg tgatgatgcc
    25201 gcctttgagg cctgtatggt ttccgacctt gatttgatag ccgtcttctc cggcataaat
    25261 accgctttgc tcggttaccg aagcatggtc ggctcggatt ttgctttggc tgtaatcgcc
    25321 actggcactg aagccataac ctacggtaac ttgtgcaccg gcgttttgtt gtttgctttg
    25381 ataagtttct ctatcttgta cactttgaat gcttaggttt ttggcattga cttgtacgcc
    25441 tttgccgcgt acttgcgcgc ctttgatgat ggtatcgcca ccactttgga taagggtttg
    25501 gctgcctttg tcgccaatat ggctatggcg gtgggttacg ctatcgccat agccgtagcc
    25561 tttgccgaca ttgccgcctg cggcaacgcc taatgaccag cctccttgtc cgaatgatac
    25621 ggcagcacct gcgttccagc ctgctgattt gttttggctg cgttcggtat tgctttgctc
    25681 ggctgattgc agatggattt gcttatcggc aatcaggccg gttcctgctc tgcctgaaac
    25741 atctgagcct gtgatgttga tattggattg ttcgccgcac cttcggcaat aagggtagtt
    25801 ttgccgcctg cttgaatttg actcgcttgg gcttgattgg cttgaacttg ggtggtttgt
    25861 cggttttgct gttcgccgta ggttatggag atgctgactt gtttggcatt ggttgtacca
    25921 ttggctaagt tttgtgcgcc tttgcctgtt tgataggcct gccaacctgc attggcagcc
    25981 gccatggcat taacgcggtc gtttttgctt tgtccgactt gtttgctgct ttgtgtgcta
    26041 cggcaatcgc ttgttgtgcc aaatcggtaa cgggcgaact gaatgccacc gttaagcctt
    26101 tttgttcgta ggtttgggtg gttttgctgt ttaattggtt ttgtgctgcg ccaatatcca
    26161 tactttgcgt gctgataagg ttgttgccct cagggctgga aacgttgctg ccggtttgtt
    26221 cgtagtgttt gcttgcaaca atggtggtat cgcctttcag gctgcctacg gtactgcctg
    26281 tatgttcgtt gctttgggat tggttttctt gtgtgtttgt cttgctgcca atagtgaagc
    26341 cgatacctgc actcatcaat cctgattttt gggtttgatg ataggtttcg ctttggcttt
    26401 gagtttgggt tgtaccaatg cgaacatgat tgcctgcttg aatccgggtg ccattatcgg
    26461 aaataacatt actgccaagg atgttggcat cgtttcctgc ctgcaataca acttgcttgc
    26521 cttcaaaggt gctgctttga gcagtttcgt gatgactttg ggctttatcg gtaatgacta
    26581 atttattacc gccgccgctt ctgcctgtat gtttggacgc atcatcaact tggccggcat
    26641 ggatgcctga gctgatagta atgtcatttt tagcatacac ggcaagtgtg ccttttgcgc
    26701 tgccgacttc ggcagctttg gcattgagat tattccctga caatagggta acatcgcctt
    26761 ttgtttgaat gctgctgccg acttcgttcg ttgaacctcg gatggtatgg ttatcggcat
    26821 caaaatggat ttcttgatat ttgccggttt gtaccgtatc caggttaatg tcgcgtcctg
    26881 cctgcagccg ggtttgccct tgatctgatt gattgctgat ttgaccggca atgatgttga
    26941 tgtctttgcc tgcctgcgct gctaaaacac ctttttcttt gcctgtgata taaatacctg
    27001 ccattcggtc taggtaggtg ctgctacctt gtgcattttg actgctcttg gccgtgcttt
    27061 ggttgttgat gttgttaccc gcattgagca ataatgtctg ttcggcagaa agaatgccgc
    27121 caatattatt gatgtcttgt gtggccgtaa ccgctgattt ttgcgcatga atacgcccac
    27181 cgatattgtc tagcgtatcg gtattgataa taagcgcatt gcgccctgca atcgtgcctg
    27241 agtttttcag gctgcctgaa acattgattt gtgtattgct gcctgacaac aatgcacctt
    27301 taccgtctat gtcgccattt ttaacgcgta cataaacctg tggcatcaat acggtttgtg
    27361 tgccgccatc aggaagttta acttcttttt gtaccaacca aacaatatcg ctggtcagtt
    27421 gcgctgcttg ctcggcactt aatgcaatgc caacgctgag attcatcgaa cgtgccgcag
    27481 tcgcgccatt atccattaag gctttaaatt gttcttcgtc gttttgataa ccgtctaaac
    27541 gacgatgccc tgtcagctct gcgatttgtt cattgattaa acgttgctcg taataaccat
    27601 cacccaaacg tttatgtaaa ttgtttgggt ctagtttgag gctgcccagc atatagtcac
    27661 tacccaacca ttgacggtag ttggcaaagc gtggatcggt ttcaacaaga tagcctttat
    27721 tggcaggatt gataatgtat aagctgctgc cgggtaatgg ggtaaaagaa ttgggcgtat
    27781 agggtagcga aataccgttg cttttcgcag tacggatatt atcccggtta ctttgtggca
    27841 actcagtgcc ttggctgggc gcatgacggc ttaatgcttt gctatgcgat tcataggcaa
    27901 atgaacccag tgaaatgtcg cgtgtgattt cctccggcaa agtataattt tgttcacgat
    27961 gccctgtttc atcatgtcct ttacgacgcg cacgccagta gttgtgcaac ttaccatttt
    28021 cgctgaagac tttcttctcg ccaaaggttt gctcgttatg caaaccgtct ttttctgttt
    28081 gcacaagcaa attgccgccg gcgatgattc ggctgtcgct gttgaagact gctttgctat
    28141 caataatcaa atcgctacct gcaatgattt tagcaggcgc agttccggtt acttgagttt
    28201 cttgcgttac tttttcataa tcgtatttat gccaattttc atgcgccact ccatcagggg
    28261 tgcgtaagtg gtctgattca ttgttgtaga caaaccagcc taattcatgt tgcgtgcctt
    28321 ctcgcaataa ttcgtgtcgt ccaaatgctt cgtaatcaac aatacgctcg cgccctgttt
    28381 ctaccaactg cgttttcaaa tgttcattgg tattgtgcag cttttctaca cctaaacgca
    28441 ttttgcctgc agcttcaatg attgcgccgg cattgtgtat cctttgggct ttgcctgtgg
    28501 cttggtcatt ggtatctaat gcgccgccaa ccgccatatc gttaccgctg taaatcagac
    28561 tgttttcacg gttatttaat tgttcaatgc ctaaattcag gttttcgcgt gccgcaatgg
    28621 cggcacctgt accgttttca tcttgattgt ctaagcgggt agccgcaata gcgatattgt
    28681 cgccataaat ccgacctgta ccgatattat tcatttgccc ggcttggatt ttggtttgtt
    28741 gtccgtcaat caagcctcta ttggttaaat tgtgctgcgt gccaatgtct gtcgtaccgc
    28801 cggattgaat gttgccttgt actgcattat caaggttatt tgctttaatc cgaatgcgtt
    28861 ttcctgcttg caaggtatgt gaatttttca ggctgcctcg tgtactgagc gacaattcat
    28921 tgcccgccac gatcttgcgt tctacataaa aatcatcttg taacgcaata tccagtttat
    28981 tatcagcggc aagtgtgccg ttgttggata acgattttgc ctgaatggca acatcacggc
    29041 ctgattgtat cgtgccattc gtattatcaa tgacaacggt agattgctga ccatcgtgaa
    29101 taatcagttg ttgattggtc gctatttcgc cattttgatt gttcaggctg cctgaaacgg
    29161 ctaaatcctc tgttcctgcc gacaataatt tgccagtgtt ttgaatcgat tgactgtgaa
    29221 tttgagtgcc ttgttgcgat accgccgtac cgctgttttc aaaggcttga ctgcggatat
    29281 tgactttgtg ttccgctgta ttattcatat cttgcacatt ggcggcagcc atcgtgccac
    29341 tattgaccaa acggccattt gcatcaatcg ccacattacc ggaagaagca aacaactgcc
    29401 cttgattacg aatgcctgct tgttcggccg tactgatcaa ggtgattttg ttggcataca
    29461 agaatacgtg tgaaatcggt atcacgggca tccaaaccgt gtccggcgat tacagcattg
    29521 ccttgcctta tcttaaagcc gctaaagtct cctgcttgat attgcggttg gcctgtcgtc
    29581 aaagtggcac gggaagcatt gataaaacca ccaccattga ctgcaatccc tgccggattg
    29641 gcaataacga cttctgcacg tcgtccaccc acttcaatat agccattcag ttgtgaagga
    29701 tggctgctgt tgatttggtt tacaaccaca cgtgcttcgc cccttgtcaa ccaaggattg
    29761 ccttgaatcc aaccgcctag ctgtgtttgg gtgttgctgc gactgttgtt taaaatcgcc
    29821 ccgcgattac ccacatcaaa ctgggcatat tgattaacag aaacccctgc cgaagtaggg
    29881 gtttgaatat tgacttgcgg tatgccgtta cctgtttgca gaatcgtggc ttgttgggtt
    29941 ttaggagcag ctttatcagt aataatgccg tcagcaaaag caatattgac cgtacccaaa
    30001 gccaaacata aagaaaagcc taatgcagaa aaacaaaagg ctttggaatg agtaggaatg
    30061 aaagaaacgg atttcacata aacgctgccc gaaccactat cggcacagct tttaccttcg
    30121 cgcttggtgg tttcggcaac agctaccaca gcaccgcgtt tgcggttgaa aatcacacga
    30181 tagagggttt tgttcatgat tttagttatt tgatttttat agagttatta caaatattgg
    30241 acagtctggg cattctagga caaagatttt ggtaagtcaa ttaccgccat ttttttatta
    30301 aattaaggga cgtaccggat aacggctaaa aatcccattt aggttagaat aagccaatga
    30361 gaaaagccgt ctaagccggc ataaacaaaa taaactcatt gggctatttg tcgcaggcgt
    30421 aactgcaaga acagcggcag agttggtagg cgttaataaa aataccgcag cctatgattt
    30481 tcatcgttta cgatgactga tttatcaaaa cggtctgcat ttagaaatgt ttgatggcga
    30541 agtagaagca gatgaaagtt attttggcgg acaacgcaaa ggtaaacgcg gtcgcggtgc
    30601 tgccggaaaa gtcgcagtat tcaggcttct caaacgaaat ggcaaggttt cctttgccgt
    30661 ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac cctgcccgcc gtttcggttt
    30721 ccgtaccccc ctgttagata agggggggaa gattcgaagc ggttgccggc ttcctgccgt
    30781 ccgccggcgc gtccgtcatc acgccggcaa ccgccttttt catcccttgc ttatcttcca
    30841 tggtgcgaat cctcaaaaac gggcaaaaaa agccctgtta cttgtagaaa gtaaaaggcg
    30901 atccgtccgg tttttatcgg gaaggcggca ttcatcatag gattgccggt attgcgcctg
    30961 ctcgtaccgc ctatgccgta cccggcaatc aaggcttttt atcagaattt ttttcggaat
    31021 aaccctccgg aaaccccata atcagaagcg tgcgggaaga aacagccgcc ccgccggcgg
    31081 ggttgcggca atgccgtctg aagccacgaa tccggcttca aacggcatct gtttaccaaa
    31141 aggcaaataa tccgatttgg cgaaaaacaa atttgctttt tagtaaatac gcgcttacaa
    31201 tccgctacat ccgatttcta caaaggataa aacgatgacc gacacagccg gcttgcgccg
    31261 ccgcaacctg cggcaatgga tagcggaaca ctacggcgga ctgcaaaacc gttttgccga
    31321 agccgttgcc ctcaacacag gcgaactctc cgcccttttg aaaaacaaat ccttcggcga
    31381 gaaaaaagcc cgcaaaatcg aacaggcggc aaaaatgccc gccttctggc tcgataccga
    31441 acacaccggc cgaccgcccg gacacacagg aaaacacacc atgtcccata tttcccccat
    31501 ccccgaaatc ctagccgaca tcaaagccgg taaaatggtc atcatcaccg atgccgaaga
    31561 ccgcgaaaac gaaggcgacc tgctgatggc ggcgcaattc gttacccccg aagccgtcaa
    31621 ctttatgatc aaacacgcgc gcggcttagt ctgcctgcct atggaaggtg caatggtcga
    31681 aaaactcggt ctgccgatga tgacccaaaa aaacggcgcg caatacggta ccaactttac
    31741 cgtttccatc gaagccgcgc acggcattac caccggcatt tccgccgccg accgcgccct
    31801 gactattcaa accgccgttt ccccgaccgc caaacccgca gacatcgtcc aacccggtca
    31861 tatcttcccg cttcgcgccc aaaaaggcgg cgtactcgtc cgtgccggac acaccgaagc
    31921 cggcgtcgac ttggcgcaaa tgaacgggct gattcccgcc gccgtcatct gcgaaatcat
    31981 caacgacgac ggcacgatgg cgcgtatgcc cgaactgatg aaattcgccg aagaacacaa
    32041 gctcaaaatc ggcacgattg ccgacctcat cgaataccgc agccgcaccg aaagcctgct
    32101 cgaagatatg ggcgacgcgc ccgtacaaac tccgtggggc gagttccaac aacacgttta
    32161 cgtcgacaaa ctctccggcg aaacccacct cgccctcgtc aaaggcacac cctccgccga
    32221 caccgaaacc ctcgtccgcg tccacgaacc cttcagcgtg atggacttca tccaagccaa
    32281 tccgcgccat tcctggtcgc tgcccaaagc ccttgagcgc gtccaacagg ccgaaagcgg
    32341 cgtcgtcatc ctcctgcacc acaccgaaga cggcgcatcc ctgctcgacc gcaccctacc
    32401 caaaggcgca aaccaagcct acaaatggga cagcaaaagc tacggcatcg gcgcgcaaat
    32461 cctcgccggc ctccacgtca aaaaactgcg cgtcctcggc caaccgtcat ctttcaccgg
    32521 cctcaccggc ttcggcctcg aagtcgtcgg ctttgaagaa gcggaaaaat aatatagtaa
    32581 attcaaatgc tttatatttg cttcatttat tgcattattt ccgtacaaac gaaaacccgg
    32641 tctgttgggt tggattttgc ttttttcgga tttcgggtaa cttctaattc gtcattcccg
    32701 cgcaggcggg aatccggttc gtcgggtttt tgtgtcattt ccgatgaatt cctgtggctt
    32761 tggttttttg gattctctct ttcggggaaa gaacggcata agtattttcc aaaccaaaca
    32821 aaatgccgtc tgaaaggctt tcagacggca ttttaagttt gaccggtttc attcatcggt
    32881 atttatgaat tgaatttcaa catcgccaat ctatccttaa tctccttttc caattcggca
    32941 gattcggcga aaagtttatc caaatccgct gaaaatcccg ctattttttg cacaaattcg
    33001 tcggcggata tatccacata atcaattttt acctcaaaat actgccccgc cgacaagctg
    33061 tgattcttcg ctttgatttc atcgtagccg accaccacgc taaaatcttc cactgcctgt
    33121 ttgttcgtga aagtattgca gattttttgt tcttcttcgc aggaaagtac ggttttttgg
    33181 tttttgccgt ctttgatttt ttcgcccaag cccgatgcgt caattaatac cactttgtct
    33241 ttattggttt tgtcgataaa caggatggaa acattggttc ccgtcgtggc gaaaatattg
    33301 gaaggcatgg acaccacgcc ggcaagcatt ttgttttcca ccaaatattc gcggattttt
    33361 ttgtcgatgt ccgattgagc cgtgataaag cccgtcggca atacaatcgc cgcttttccg
    33421 tcttctttca ggctaaacaa gatatgctgg ataaaaagct ggtagatttc cattttatcc
    33481 ttatccttcg ccttaatttt cgggataccg gcaaaaaaac gttcgcggtt ttcatcgctt
    33541 tccaactgat cgcgaaaatc gctgaaatcc aatttaaacg gcggattgga aacaatgaaa
    33601 tcaaattttt tcagacggcc tgaagcgtct ttgtgatacg gcgacaaaat cgtattgccc
    33661 tgaaccacat tattcagcga atgcaccaga ttgttcaaac tcagattcag ccgcaacaaa
    33721 ttggacgatt tctgcgaaat atcctgagta taaatcatgc atttatcttc gccgatgaca
    33781 tgggcgacat tcatcaacag cgttcccgaa ccggccgacg ggtcgtaaac gtccacgctg
    33841 cggatttgcc cgcgcacctc ctccggcacc aaaatatccg ccataatccg cgcaaccgcg
    33901 tgcggcgtgt agtattcggc atatttgccg ccggaattac tgttgtagtc tttaatcagg
    33961 tattaaaaaa tcgtggcgaa aaagtcgaat ttttgcgcaa aaatcgcctc gaagctgaaa
    34021 cccgccagct tggaaatcag cgcgcggcaa aaatcgtcgc gtcggccttc gtcggtaatg
    34081 tactgactga tacgctcgaa caacacgatt ttcgccccgc cttcggtttt caccgaaaac
    34141 aaatccgcat tacaggcggc aatatcggtg agcgtatcgt caaagagttt ggcaaaatcc
    34201 gcgccgtttt gccgctctga taaatacttg atggaatgtt cgggctttaa aaccgctgtt
    34261 tttccatcaa tatccatatt gacgaactcg atcggctcgt cgggcttttc tttgcgaatt
    34321 tttttaactt caaaatcgta tttgtcgttt aggaatttat acaaaaacgc ctgactgatg
    34381 attttaaatt cgttgccgtc attgcccaaa ccatagttgg cgcagatggt ttttaagctg
    34441 tcaatcaagg ctttggtttg ttcggtaaag tgttgttcgg tcatggaatg tctcggtttg
    34501 ttgctagcgt cgcttaaatt ttgtttagca ttagttggtt tgctggcaga ttaaataggt
    34561 tgaacactat ttgccaacct tttctctttt cctaatgctg ttgtgtaata acgttcatca
    34621 ataccatata acaaatgttt cagctgtaca tcagtagaaa tttctacacg agagccatct
    34681 gcgttaaatt tcaagatatt attattatat tcagcaaggt aatttaatag ttcttgtctt
    34741 tgatctagcg tcatattatt caatctatct tgaactaaag ctattctttt cctattggga
    34801 atagatactt tttcacttaa aaaatcttct tttaaatcaa taaaatcgct ttctaagaat
    34861 tgctgtactt cagtatttgt agcttctcga tacaaatctt caatcccttt aaatatattt
    34921 gaaatagaag aaagggttct aaatattaat ttatcatcct ttattatata aacagcatca
    34981 ggaatatctt taataaccaa taagtcttgt ggttcgcata gtttcgcacc atttccgcat
    35041 atagttagca agtgcttctt tttgagtaat aatgaacttg tgattttctg gaagaatagt
    35101 gcatcattag tacaagatac taaatacttt agtttctcta tatttatttc ttcctttttt
    35161 atttcactaa acatttttga atcaaataaa tttgagttat ccaaaaactt aggacaaaaa
    35221 tcttgattct ttaaattttc aatttgaaac caagtatcat catcaagatt gtaatcatag
    35281 ttatatgtta taaaatttaa attctcgtaa gaaaagtcag ggaaaatatg atcatcagat
    35341 aatattttta ctaaatcttt cttgcaagta attttagcta aaacaaaatt cattattgaa
    35401 ctcctatttt tctaagaaag taaaattatt aattcgatat acagcaatcg aacttacact
    35461 ttgtggcgtt ctaaattcct gtttactaat cagcactaat aataaccctg attcagtttt
    35521 tgcttgataa aatcgatagc caaataagaa aaaaacaggg ttaaaataaa atgacttaga
    35581 gcatgaaact agtaagaaaa taattccata cataacaaat agcaaaaaaa gattattatc
    35641 gggtatgctt agagcaacaa aaaaataacc aagatagcta ggtaagaaat tatcgttaac
    35701 atattctaat tctttcacct ctccttgctt gaattcatcc ttccccaagt atttgcttag
    35761 ccataagaca gtagcggttg cgataaatgg tattagaaaa taaaaaagta gacttaactt
    35821 agaaataggt tctatccaat gatttgaaat tacacgataa aaccaagtaa ggtctaatat
    35881 ataatattgc gatttaataa aatatattga taacagcaaa gatgtactac ttatatttaa
    35941 taatatttta aatagtttat ccatctcttg ctccgctaca tcttacggat acctgcccgt
    36001 tcatcagcat gggtagtagg aaatcgcgta gttgggtcag gtggtggttt tgttttagat
    36061 tatttcgaac tttaacaaag aaagtatttg ctatttcatt atatttttga gaaatatctt
    36121 tactaggcaa aattatttta aattctttta agaatttaaa atgtctagca taaccataac
    36181 ttgataaatc tatttgattt atgatttggt aaaacaaata gtttggcatt cgttcattat
    36241 tgcttaatat tacttgtgta ccatctgctc cccgcgcata ttgaaaatta actaacttaa
    36301 caattcttgt atgatcacca aaaataatat gagcatcttg tgggtttaat atagattttt
    36361 catcattggt aaatccacaa ataaaatctt gactttggtc aacaacagga tattttccaa
    36421 aatccttaat atctttattt aatatttttg tagtatttgg gatttttgct aagcaagatt
    36481 gtaattctat acttccccac cccttcggaa tttcccgttt caaggtttcg tcaaacacca
    36541 tgtcgccgcc tgaggatttg tagggcttgc cgttggcgtc ggggaaatcg aactgcacaa
    36601 accagtagtc gtacagggtt ttcgccatct cttccaggcg cgcgttgatt tgtttgttca
    36661 gggcgatttt tttgtccaaa gcggatagga cggcggcgat ggattgttgg atttgaggtt
    36721 ctggaatagg caactccagt gtttttaatg cattttcatt gacacgttgc ctgccggaag
    36781 ttccttccat acattcaatc gctcgttttc taaaatcggg actgattgca aaataataga
    36841 gaaattcggg attggtttca ttttttgctc ttaacacgat aaattcggta gaaccgaaag
    36901 ccacttctcc atcatctaaa atatctacaa aagcggtttt cccattttcc aaacacgggg
    36961 taatcttagc taataaggta tcaccattgc gaaacttagc tccaccgtta aatgccttga
    37021 tttcgtaacc cgtaatttgt cgttgaaatt ctttcaacat tgccatcgga acactttttg
    37081 ccaatgctcc cttggctagt tgctctctcg gattaaaatc agcaatattt tgaatttgtt
    37141 gtttcttaaa atgattcatg atgttccgtt acaaaactct tcaaaggccg tctgaaaaaa
    37201 acatttcatc ggtagttctt gccgtctgcg gcagttgtaa actgaaagtt tacaactgaa
    37261 tcgtccagtt cttgttctgc aaatatggct ttgatgtgtt taccgatgtt ttgtttgctg
    37321 gtttggtaga gttcggcaat ttctttttgg gtaagccata gttcgccgtc aagctcgcgc
    37381 agggtaaatt cggatagtcc gtcttccgtg gtgtagatga tgatgtcgtt gttgtgcata
    37441 gctttcctgt atttataaat ggttaaggct acctaaaaaa cctctttctc aaatccgccc
    37501 gctttctttt aaatattccc gcaccaacag gcggttgatg gcttggatgt ctgcaggctg
    37561 aacaggaaat tgctgttccg ttctaaagcg ttttaatacg atgccttgca tttgtttttc
    37621 aaagtaattt tggttgtcca aaatctgctc catatccagc actttttggt cggcatcggt
    37681 ttttacaccg cttagcgcgt taaagacttt gagtttgtct ccgtagaggg cggcgttttc
    37741 catcaaccgc ttgtggatac gtgcgtattt ggcgtcgccg ccgtatttgt gccgtaagag
    37801 gtcgttttgg cggttcagct ctttgatttt ggcatagacg gtttcgagtg tggcgatgtt
    37861 tgtctgcatt tcttcttgcc cgacttcggc gaggttcttt ttcttgaaga tgcgttccag
    37921 ctcttcgcgc aggctgatgt attcaaggtc gtcttggtcg aagttgcccg ccagcccttc
    37981 gcgcactttg cgcatgatgt cttttaaatc gtcggcaagt ttgagttccg cctcgccgat
    38041 tttgacgaat tgaaagtaaa cgtcttccag cgcttcattg agcaaatggg cggtgtcgcc
    38101 ttgctgtaat ttttcggcaa ggtttaaggt atccaagcgg gcggcggttt cgcggtacaa
    38161 aagattgagc ttgtcaaaat ccagatgcgc aagaaattcg tggctgcctt gcaggcgcag
    38221 gatgttgtac aactctttgg cggtttgcag ggcttttttt aaggctagaa gctgtttttt
    38281 gtcttctatt tggctaattt ggctgcaaaa ttcttcggcg ttttcggtat cgaaatcaaa
    38341 cagggcgttt ttaatgtctg caatttcctg ttcgatttct tcggcggttt tgaatagctg
    38401 gctgtaactg ccgatttcgt cgcccaattc gttggaaagt tcgtcccaat aggcgcggtt
    38461 ggttttgtca aattcgcgtt cgatatcggc aaaatcgacg acatagccgt agcggtaaga
    38521 tttgtaggtg cggttgacgc gggtcagggt ttgtaaaagg ttatgggctt ttatcagtct
    38581 gcccaaatag agctttttca ggcgcggcgc gtcaaagccg gtcaaaagca tgttgtacac
    38641 aaacaagatg tcgattttgc ccgctttgaa atctttaacc cattggtcgc gttcttcctt
    38701 agtgccgacg tcgtgcaata tcaacgcggc ggtaaaattg tggtctgagg ccgtctgaaa
    38761 atgctcaaaa agctggcgtg cctgttctgc gctgtcgcaa acgaccatgg caccgaggct
    38821 ctcgtcttgg ttggtttttc tgaatttggc gaagtcgtcc aagatgtagt caagcatcgg
    38881 gtggacgaag tgcggatggg cgtagatttc tttgcggtca aagctgcctt tttcgatttc
    38941 aagttgcgcc agtgcttctt gtaattgcgc cttgtatcgg ctgccgattt cttcgcgtat
    39001 caggcgcagg gtgtagccgt cggcaatgga ggcgttgtaa tagtatttgt ggatgtagtc
    39061 gccgaagagt tcgcgggtgt tgacgttgcc tgcggttacg ccgataagcg gcgtgccggt
    39121 cagcccgatt ttgacggcgt ttacgtcgga ctgattaagg ttggcaagaa atgagccttt
    39181 ggggttgtag ctgcggtgta cttcgtcgag aaaatagacg cgccggatgg cgaggtcgta
    39241 gtcgttacgg gcgacaacac gggggcgtct ttgaattttt ggatgttgac gacggtgatt
    39301 tccggtttgc ctgcgtggtt gtgcagggtt tgggcggatt tgatgtcggc aacgaaggct
    39361 tcgcggctgt cgatggtatg gacgattaag tcgcgggcgg taaattcccg ctgcgcctgt
    39421 ttcaataaat cgagcctgtc cacgatgaaa tagaatttcg gcacgatacc ctgtttggcg
    39481 taatagtggg tcaggtaacg ggtgttgtaa taggcaggcg cggttttccc cgatccttgg
    39541 gtatgccaaa tcacgccttt tttaccgccg ttcgataaat gtttttcaat ggcaagggtg
    39601 gcaaacagtt gcggataacg catgatgtgt ttttgcaccg aaccttgact tgctttgacg
    39661 taagtcagcc cgtgttgcag gagaaaggca agacgttcgc ggcaaagcag cgaagtcagg
    39721 atgcggttgg tcggcgtatc gggcgattta ttgctgataa attcggggct gtgtttgata
    39781 acgggcagat tattgtcttg tagaacgttg ttttcaaggt cgtctgaaag cgtgtccaat
    39841 aattcggtaa ggtttaattt atgctcttcg cggaagtagt tgaatacggg cttgccgcaa
    39901 gcagacgagg cgtaaaacgc gccttgtgcc ggctcggttg cgccgtcgtc gtattccatg
    39961 ttgttggaaa aaatcatgaa ttgggtgatg ttgataaaac ggcggaattt gggatttttc
    40021 gcacgtttgc ccatgcgctc ccgctcttcg ccgatgccgc ctttattgtt gggctttttg
    40081 acttcgataa agaccaaagg catgccgttt accagcaggg caatgtcggg gcggaatgct
    40141 tcgtcgccgt tgatacaagg caactcagtt acaacgtgga aactgttgtt gtcaaaattt
    40201 tggaaatcga tcagcttctt accgccggat tgattggtga ggcgttcgta aaatttttgc
    40261 cccaaatctt cgttatccag ctctaagcgt atgtcggcaa gcaggcggcg tgcatcatcc
    40321 ggcggcaaat cgggattgat gcggcagagg ctgtctacaa agatttcggg aaagatattg
    40381 gtttgtctgt cccaatttgc attttttagc gaaagataat catagcccaa gcgcatcagg
    40441 tgcaaaacgg cggggatttt gacgcgggaa ttttcgttgt gggtttggga cataaacgta
    40501 acgtaatggg tcatcaaaat ttattgaaac ggattataac acctgaaaaa agccgatacg
    40561 ccttttcggt gtaccggctt tgccatactg ttctgcttca gacgacatcg cttcattttg
    40621 cctttaatgc ctcttcgtcc agcgatttca accatgccag cttttcgccg attttgattt
    40681 ccagcccgcg cgggacgggt tggtaaaagt ccggttcgtc caagccgtcg ggcatataac
    40741 tttcgccggc ggcgtaggcg ttcggttcgt cgtgggcgta gcggtattcg cgtccgtagc
    40801 ccaattcctt catcagcttg gtcggggcgt tgcgcaggtg gacgggcact tcgtcgctgg
    40861 cgttttcttt gacgaagcgg cgcatttggt tgtatgcctt gtagcccgcg ttggatttgg
    40921 cggcggaggc aaggtacagc acggcttgcg ccagcgcgag ttcgccttcg ggcgagccta
    40981 agcgttcaaa ggtggcggcg gcatcgttgg cgatttggaa ggcgcgcggg tcggcaagcc
    41041 cgatgtcttc ccaagcgata cgcacgatgc ggcgggcgag atagcgcggg tcggtgccgc
    41101 cgtcgagcat acggcaaaac caatacagcg cggcgttcgg atgcgaaccg cgcacggatt
    41161 tgtgcagggc ggagatttgg ttgtagaaac tctcgccgcc tttgtcgaaa cggcggattt
    41221 gcgcgcccaa gctgtcggcg agaaattcgg tggtcaggat tttcagacgg cgcgtgtcgg
    41281 cggcacgtaa aagttgttcc aacaaattca ataatctgcg cgcatcaccg tcggcggtat
    41341 tgacgagcag ctcttgcaca tcggcttcaa tcgtgaaatc ccggtattcg ggcaaagcca
    41401 acactttggc aatcagtttc ttgaggtcgt ctgaagacaa ggattgcaaa acatatacct
    41461 gcgcgcggct caacagcgcg ggattgactt caaacgacgg attttccgtc gttgcgccga
    41521 taaaggtcag caaaccgctt tcgacatgcg gcaaaaacgc gtcctgctgc gccttgttga
    41581 agcggtggac ttcatcgaca aacaaaatcg tcgcgcgtcc ctgctgcaaa gcgatttcag
    41641 ccttatcgat tgccccgcgg atgtccttca cgccggaaaa cacggcggaa acaggcaaaa
    41701 actgggcatt gaaactctgc gccaaaatcc gcgccaacgt tgtcttgccc acgcccggcg
    41761 gcccccacag caacatagaa tgcggcttcc cgccttccac cgccacgcgc agcggcttgc
    41821 cttcgccgat taaatgctgc tgcccgatga catcatcaag cgtatgcggg cgcagacgtt
    41881 cggcaagcgg tgcgtcgggt tctcggacaa acaaatcggt cataacggct ccgtcaacag
    41941 gttttcagac aatatgatta tacggcaggg aaaggcggcg tgccgcatac ggataccgcc
    42001 ccaccgtttg tactaagccg atcttaggcg tacaattggg aaatttcagg aaatttatac
    42061 gctatgccat ccatgcagct ttgcaaccgg acccgcaaag accaggtccc gttttaacca
    42121 tgcggggaag ttaagattac agtaaacaaa actattttgc cttattggaa gaaaggggaa
    42181 cagttatgag aaattggaac ttattaccac cggggaaggg gcagatggag acttactggt
    42241 caacggttct tacggcatcc gttcttttgc agacctaaaa ttggaataca aacccgaatt
    42301 atcggtgaga agcaatcatg acattatttt caacaatgat tttcaatctg ttgacgtaca
    42361 accctattcc ggaaatatcc atttggatgc caaaggaaaa attcaattta atttgaatgt
    42421 tgccgataag gtaacagatc caaccacaca tcaagaaaag ttagtgaacg ataacgcttt
    42481 agtgcggtat gtcaacaata tcaccccctc cgatgaaaat gacaaaggca gcatatcctt
    42541 ccattcaaaa acaggcaatt tgttcaatat gaacgttaac gttcataaag gcacacacca
    42601 tttcggtatt cacaacctcc gcaataccaa tgttaatctg actgccgaag acggcgataa
    42661 tgtggttgcg attaaaggga agcttgccga gcctatgccg gcaggaaaag ttgagagtac
    42721 aagtttttta aggggtattg aaacggcttt taaatccaat accgtattaa cggggaataa
    42781 caataaaata tccatcacca ctgagggcga cggcttggga ttgggcgtaa atggcctcca
    42841 gtccaactcc ccctattccg gacacacgac gggtactttc atcaataatc aaccaagcaa
    42901 tactacccaa ccttcccttg acggcaccag caccatcgaa ctcaaagccg tcaaggggaa
    42961 taatgaagtt gatttaaatg taaaaaacca cgcctccgta aaaggcatta tcacgtcgca
    43021 ttctgcaaaa gcaacgcttg aagccgagga ggataatatt gtccgggtta aaaatccgga
    43081 caccaagacg gctttaatca atgcctggaa agaaaaatac cccaataatt cgccagaacc
    43141 atacagggtg gggggttcaa tccacgattg acagcgatgt agttttgagt gcggcaaaca
    43201 atatcgttga agtaggaggc agcccgatga accaagaagg gattaccgct cacggaaatg
    43261 ccacgattac cctcaaggcg aaagaaaaca ataaaattac cgtggaaaac gccgcataca
    43321 gcagcgacgg catttcgact ctgattaaca gaacgggggc aagacccgga acaagggatg
    43381 atggaaataa aatcatactg gaagccggcg gcgataatat tgttaccatg aaatccggcg
    43441 atgcggatgc ggattatgta aacaattcca aagtattaac ggagacacca tattataaaa
    43501 gcaaacgagg ttccaacggc atttttgcct atggcgacaa atcgctggtc aaactgattg
    43561 gcgagaataa tatcgttaag agtgaaatca gtgaaaaatc taaggcatta aatgggggat
    43621 ttcgccatat cggcatttat tcatggcaaa acgcgaaagt cgaattgtct gcgaagagcg
    43681 acaatatcgt acaaggcgga atttggggct tatactccaa caactcctca atttccctca
    43741 aggggaaaaa ataatgtgat ttcaaacccg aaatataatg ttttcgccta caaaaaggca
    43801 aaggtggatt tggtcaaatt accattgact ccaacgttgc caatcttgca aggcaagatg
    43861 gtttaattca tttgaattat aaagacgata cccgtatcac aggggcaacc gtatctgata
    43921 agggtttggt agccatcaaa cctttgaata acacgaatat tgttgccgac actattcact
    43981 ataaaggcga tgtcttggcg gtaaataagg gtaaagtgga attagatttc acgccgaaca
    44041 tccgtttagt gggacgtttg gataatttta gcggcttaac cgattccaaa cataaaaatt
    44101 tattcgaaaa ctatgttgca aatttagaca gcaaaagtgc gggcgaaatt aactttaatt
    44161 tagccaaaga cgcattatgg acgatgacag gtcaaagctg gctggataaa ttggaaggac
    44221 aaggcactat cgattttaat aatgatgcta aaacaagtgg acgcgcctta catatcggtg
    44281 aattggcggg tgccaataaa ttcttgatgc atctgaataa agacggcatt cacagcgata
    44341 tgctctatgt gaaaaaaggc acttcgacac cgcaagaagt cgtcgtcaaa aatctgtccg
    44401 aagtgctcga cagtatgaat tacggcgaac gtttgcgttt cgctacagta acaaactcaa
    44461 aaaatgaatt tgtgaacggt aaaaaatata ttgacgatac gcaccttatg gaggatgccc
    44521 tgactgtcga atactccgca cataacggcg ataaaaacaa caaggatgac tataataaat
    44581 cctttaacgg ctctgaaatg acggcggaaa aagctggaga cgattatgtc aataaaacct
    44641 ataccgacaa caggcaaaat gtctatttgg tcaaacaggc taccggcaat ccgagccgaa
    44701 atgtcaaaaa tatcaatgat atgttcgatt caaccgcaca ttatgcgttc actttggata
    44761 cttatgccaa acgcgaaggg gagcgggctt tttcaacgtt ggataaaaaa gaaggcgatt
    44821 ggataaggct gacgcatacc cgtgtgattc aatccaatgc gtttaggttt cataacaacg
    44881 attttgaaat cggatatgac cgattcagcc tcaacgagca ggagaaaaaa cgcaaatggg
    44941 gcataagtct cgactacggc cacggcagga catcattatg gaatacgttt ggcaaggaca
    45001 aaatcaggaa atatgaattg gctctgtaca atactaccca atacatagat aaagaaggag
    45061 acgaaacagg gtatatcgac aatgtattaa aaataggaaa actgcgtaac cgtgtgattg
    45121 cacgaaatca tatggggcaa ttatggggca agggaaaata tagcaacacc ctattctcta
    45181 tcagcaccga atacggccgc cgtaaatttt tggatgacga taaattgtgg cggattacac
    45241 cgcaagtaca gttgcaatat tcctatttga gaggtaccgg ctatcggatc gataacggca
    45301 taaacatcaa tttaagccac gcaaacagcc tgataggccg cttgggtttg gatgtcgtga
    45361 gaaaatttga cggaggcaaa aaacttttct atatcaaagg caatatcttt catgaatttt
    45421 tgggcagtcg ttcctttaag gcatttgagg gcaaaagtca ttatgctcaa aaatgaaata
    45481 cgagggggac ttggtattcc gccggattgg gctacaatgc gcggacaggc aaaaaaaacg
    45541 aatgtctttg ccgatgcgga aaaaagagtt ttccggcggg aaaaaaggat cgtacaacat
    45601 ccgcttatcc gtttcccatc agtttggctg aatgatatcg gggccgcgcc attattcggc
    45661 aggccccatt ttattgccac acattgcctg cccgattccc gtttcaacag caaacccttc
    45721 caatattttg ttcaaacttg gatcgcggca gatgggggcg tgcaagcccc gcaaggtttt
    45781 tttggctttt acggaacagc acgcatttcg ccaaacggca aaattgcatt aacggaattg
    45841 aaaacttttg gaattgtgca aaacggcatt tgcgtcagtt taacggcatt tccaaagggc
    45901 attttaagcc gtattcgaga gaatgcggat ggcgttttca gtgttttcag cgtgataaaa
    45961 ttcaaatttc tattctaaaa caatcagcaa agcaggattt atttcagttg tccgagacaa
    46021 cgggcggctg caccgcctgc actccccata ctttttcaac gcactcgagc aggagtgaga
    46081 cgtggggatt atcctcgtat tcttccgcat aaatgaaatt gagcggcata ctgtggcggc
    46141 acgattcgat aatagccacg cccgcgcctt cttttgccgc cgcttcggct ttgtttccct
    46201 gcaccattgc cacacctata ccgcctgcaa ccaaatcgat aagggtttgg gggtagtcgc
    46261 acaatatctg tttttttggg tgagagccgg ttgctgcgcc aaaactggtg caggtgcttc
    46321 ctacttccgg acacgcccga catttctatc catacgcatt cttgcaggct ctgcggaagg
    46381 gagcgggtca gccgggaata ttggctttgc gggcaaatca gcgcgtaggt caggttttgc
    46441 aggaatacgc tgcggatgcc gccttgggcg gcgttgccga gtataaagcc gccgtgcagg
    46501 gttttgtgtt ggatgcgcga gaggatttcg ccgctcattc cgtattggat gtgcaggcgc
    46561 gttttggggg gctgtttgac cgatattgtc cgtcagcgcg acgagttttg ccgaatcgat
    46621 ggggtggata atgcccaaac tggtctcttc cgaataatcg cctgccagcg ttttggcaaa
    46681 atactccaac ttgtgtttgt attgcagcag ggattccgct tcgggcaaca gtatttcgcc
    46741 cgcccgcgtc aataccatgc ctttccccgt gctcctgaac agcggcgtgc cgacatattc
    46801 ttcaagggct ttaatttggg cggaaacggc gggctgggaa aggaaaagtc gtttggcggt
    46861 ttgggtaagg ttgccctcgt gcgcgacggc gacaaatgat tttaattgta cggcatccat
    46921 gtatccctcc ttgtgcggat gttttctata tttgtgcaat cgaaatcttt taggcggatt
    46981 gttgctgaaa attaactttt taatcaagtg gtttgtaaat tgtatcagtt ttccggatga
    47041 tgattattaa aaaaaaattg gttttattgc ctgtttgggc tttaaatggg gttacggctt
    47101 ccgaacgcag cccgtatcaa aaagaaaagt catgcgcccc cttttacgag gcgcgatata
    47161 taaggaggaa ggttatggaa aaacataatg ggacttatcg ggatttacac cgtccggctt
    47221 cggaatttgc gacgcgggac gaatatttgg aacatgaatt gcagattatg caaccaaaac
    47281 gctggcggcc caacctgccc tttcgcgatt accgcttcga gtgggaggat ttgattcctg
    47341 cgatggcggg aacgattgga
//
LOCUS       DAXFHF010000007        44034 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXFHF010000007 DAXFHF010000000
VERSION     DAXFHF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 44034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44034
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.007"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(258..1043)
                     /locus_tag="ACFIRM_000321"
     CDS             complement(258..1043)
                     /locus_tag="ACFIRM_000321"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3028028.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1287..2102
                     /locus_tag="ACFIRM_000322"
     CDS             1287..2102
                     /locus_tag="ACFIRM_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC3028029.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2143..3012
                     /locus_tag="ACFIRM_000323"
     CDS             2143..3012
                     /locus_tag="ACFIRM_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC3028030.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3048..4304
                     /locus_tag="ACFIRM_000324"
     CDS             3048..4304
                     /locus_tag="ACFIRM_000324"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC3028031.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(4362..4502)
                     /locus_tag="ACFIRM_000325"
     CDS             complement(4362..4502)
                     /locus_tag="ACFIRM_000325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028032.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            4650..5078
                     /locus_tag="ACFIRM_000326"
     CDS             4650..5078
                     /locus_tag="ACFIRM_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3028033.1"
                     /translation="MNFALSVITFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5127..6470)
                     /locus_tag="ACFIRM_000327"
     CDS             complement(5127..6470)
                     /locus_tag="ACFIRM_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC3028034.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6475..7128)
                     /locus_tag="ACFIRM_000328"
     CDS             complement(6475..7128)
                     /locus_tag="ACFIRM_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC3028035.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7278..7568
                     /gene="gatC"
                     /locus_tag="ACFIRM_000329"
     CDS             7278..7568
                     /gene="gatC"
                     /locus_tag="ACFIRM_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC3028036.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            7631..9076
                     /gene="gatA"
                     /locus_tag="ACFIRM_000330"
     CDS             7631..9076
                     /gene="gatA"
                     /locus_tag="ACFIRM_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC3028037.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWCSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAELVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9073..10005
                     /locus_tag="ACFIRM_000331"
     CDS             9073..10005
                     /locus_tag="ACFIRM_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC3028038.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVAKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            10048..11478
                     /gene="gatB"
                     /locus_tag="ACFIRM_000332"
     CDS             10048..11478
                     /gene="gatB"
                     /locus_tag="ACFIRM_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC3028039.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLSGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11705..12715
                     /locus_tag="ACFIRM_000333"
     CDS             11705..12715
                     /locus_tag="ACFIRM_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC3028040.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGR
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12845..13477)
                     /gene="pdxH"
                     /locus_tag="ACFIRM_000334"
     CDS             complement(12845..13477)
                     /gene="pdxH"
                     /locus_tag="ACFIRM_000334"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC3028041.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13537..13689
                     /locus_tag="ACFIRM_000335"
     CDS             13537..13689
                     /locus_tag="ACFIRM_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028042.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13698..14747
                     /locus_tag="ACFIRM_000336"
     CDS             13698..14747
                     /locus_tag="ACFIRM_000336"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3028043.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14806..16347)
                     /locus_tag="ACFIRM_000337"
     CDS             complement(14806..16347)
                     /locus_tag="ACFIRM_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC3028044.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYVGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQSAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(16849..18204)
                     /gene="xseA"
                     /locus_tag="ACFIRM_000338"
     CDS             complement(16849..18204)
                     /gene="xseA"
                     /locus_tag="ACFIRM_000338"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC3028045.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            18401..19222
                     /gene="nadE"
                     /locus_tag="ACFIRM_000339"
     CDS             18401..19222
                     /gene="nadE"
                     /locus_tag="ACFIRM_000339"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC3028046.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(19293..19766)
                     /gene="dnrN"
                     /locus_tag="ACFIRM_000340"
     CDS             complement(19293..19766)
                     /gene="dnrN"
                     /locus_tag="ACFIRM_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC3028047.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            20064..20396
                     /gene="trxA"
                     /locus_tag="ACFIRM_000341"
     CDS             20064..20396
                     /gene="trxA"
                     /locus_tag="ACFIRM_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC3028048.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(20448..21395)
                     /locus_tag="ACFIRM_000342"
     CDS             complement(20448..21395)
                     /locus_tag="ACFIRM_000342"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3028049.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            21723..23111
                     /locus_tag="ACFIRM_000343"
     CDS             21723..23111
                     /locus_tag="ACFIRM_000343"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3028050.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSASGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            complement(23225..23331)
                     /locus_tag="ACFIRM_000344"
                     /pseudo
     CDS             complement(23225..23331)
                     /locus_tag="ACFIRM_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23462..23662)
                     /locus_tag="ACFIRM_000345"
     CDS             complement(23462..23662)
                     /locus_tag="ACFIRM_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028051.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(23676..24230)
                     /locus_tag="ACFIRM_000346"
     CDS             complement(23676..24230)
                     /locus_tag="ACFIRM_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028052.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(24315..24815)
                     /locus_tag="ACFIRM_000347"
     CDS             complement(24315..24815)
                     /locus_tag="ACFIRM_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028053.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            24863..25120
                     /locus_tag="ACFIRM_000348"
     CDS             24863..25120
                     /locus_tag="ACFIRM_000348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028054.1"
                     /translation="MKVTKKPPRFRAACFAYGMDFKQAQKAPHVCVVPIAVSGCKRFA
                     LHDEMPSEDKNIGEILNQNAAAPQALYRNFFVFFSYPVENI"
     gene            25219..26412
                     /locus_tag="ACFIRM_000349"
     CDS             25219..26412
                     /locus_tag="ACFIRM_000349"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC3028055.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(26519..27763)
                     /gene="clpX"
                     /locus_tag="ACFIRM_000350"
     CDS             complement(26519..27763)
                     /gene="clpX"
                     /locus_tag="ACFIRM_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC3028056.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYCLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            27946..28317
                     /gene="rbfA"
                     /locus_tag="ACFIRM_000351"
     CDS             27946..28317
                     /gene="rbfA"
                     /locus_tag="ACFIRM_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC3028057.1"
                     /translation="MKKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     ITRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            28328..28852
                     /locus_tag="ACFIRM_000352"
     CDS             28328..28852
                     /locus_tag="ACFIRM_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028058.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            28910..29830
                     /gene="truB"
                     /locus_tag="ACFIRM_000353"
     CDS             28910..29830
                     /gene="truB"
                     /locus_tag="ACFIRM_000353"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC3028059.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDGLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            30047..30515
                     /locus_tag="ACFIRM_000354"
                     /pseudo
     CDS             30047..30515
                     /locus_tag="ACFIRM_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            30803..31138
                     /locus_tag="ACFIRM_000355"
                     /pseudo
     CDS             30803..31138
                     /locus_tag="ACFIRM_000355"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            31342..32208
                     /locus_tag="ACFIRM_000356"
     CDS             31342..32208
                     /locus_tag="ACFIRM_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003788484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3028060.1"
                     /translation="MVRTAASSSVKKLADEKKEICQQQFFSVISKRDKLLYIVKSDYS
                     KDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLEAEGNVELKNGKKPESLIETIIKLA
                     TNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERMKKVIDGEQGGISK
                     AVNWQGGGEFVYAELSPFNETAKQQILACENSDGIKTLFEGLCERHFLKYNVSVNEFS
                     QIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLSEMDDEQFAGCLNDDDKALSRAFYQ
                     SVKHQAEKKDGE"
     gene            32198..34972
                     /locus_tag="ACFIRM_000357"
     CDS             32198..34972
                     /locus_tag="ACFIRM_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC3028061.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENAEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLSDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPICAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLTT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(35121..36293)
                     /locus_tag="ACFIRM_000358"
     CDS             complement(35121..36293)
                     /locus_tag="ACFIRM_000358"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC3028062.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(36414..36656)
                     /locus_tag="ACFIRM_000359"
     CDS             complement(36414..36656)
                     /locus_tag="ACFIRM_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028063.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            36643..37089
                     /gene="iscR"
                     /locus_tag="ACFIRM_000360"
     CDS             36643..37089
                     /gene="iscR"
                     /locus_tag="ACFIRM_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC3028064.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            37118..38332
                     /locus_tag="ACFIRM_000361"
     CDS             37118..38332
                     /locus_tag="ACFIRM_000361"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC3028065.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            38317..38628
                     /locus_tag="ACFIRM_000362"
     CDS             38317..38628
                     /locus_tag="ACFIRM_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028066.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            38934..39428
                     /locus_tag="ACFIRM_000363"
     CDS             38934..39428
                     /locus_tag="ACFIRM_000363"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC3028067.1"
                     /translation="MQPITGNFDLAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFA
                     NFAFIENESRKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLF
                     MTQLAIKNGFQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQ
                     TTIF"
     gene            39441..39827
                     /gene="iscU"
                     /locus_tag="ACFIRM_000364"
     CDS             39441..39827
                     /gene="iscU"
                     /locus_tag="ACFIRM_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC3028068.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            39914..40234
                     /gene="iscA"
                     /locus_tag="ACFIRM_000365"
     CDS             39914..40234
                     /gene="iscA"
                     /locus_tag="ACFIRM_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC3028069.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(40194..40415)
                     /locus_tag="ACFIRM_000366"
     CDS             complement(40194..40415)
                     /locus_tag="ACFIRM_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC3028070.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            40497..40997
                     /gene="hscB"
                     /locus_tag="ACFIRM_000367"
     CDS             40497..40997
                     /gene="hscB"
                     /locus_tag="ACFIRM_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC3028071.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            41095..43845
                     /gene="gyrA"
                     /locus_tag="ACFIRM_000368"
     CDS             41095..43845
                     /gene="gyrA"
                     /locus_tag="ACFIRM_000368"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC3028072.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT        11709 a        12032 c        10687 g         9606 t
ORIGIN      
        1 attccccggt gtctgttttc tgccacagtc gatttagatt ccagcaaacc gcctttaaac
       61 aattgaaaga aaagggacaa attgcacagg gttctccgtt taaccagata cggatttcca
      121 ttcccggcaa tttcgggcat ttgaccgttt ccgccttcct gccccgccgc caataaaaat
      181 gccgtctgaa atatcggcac gatacttcag acggcatacc cgcccgtttt cccgcatttc
      241 cgggagctgg ctgaaattta aacgtgtgcg gaaatgattt ttaacatttg cgccagtact
      301 ttgggattgg cagccacaat atcgccgctt tccaaccaag cgtcctcacc cgacatatcg
      361 gtaacgatgc cgcctgcttc ttggacaatc aatgcaccgg cggcaatgtc ccacggtttg
      421 aggttgaact cgaaaaagcc gtcaaaacgt cctgttgcta cggcgcacaa atccaaagaa
      481 gccgcgcctt cgcggcggcc tccggcggtt tttgccaaaa agtctttcaa aatcgccaga
      541 tatttgtcca tcatgctttg atcgacaaca gggaaaccgg taccgatcag gcagcggttc
      601 aattcgatgc ggttggagac acggatgcgg cggtcgttaa gcaacgcgcc tttaccgcgc
      661 gaagccatgt acacgtcgtt acgttcgggg gcgtacacca aagcttcctg caacacgcct
      721 ttgtgcagca gtgccataga gatggcgtat tggggatggc cgtgaaggaa attggtcgtc
      781 ccgtcgagcg gatcgataat ccattcgtac tcggctgtag ctttgccgtg gaagccgctt
      841 tcttcgcaag tgattttgtg atgcggatag gcttctttca aagcctcaac caggatgatt
      901 tcggaattgc ggtcaacatc ggaaacaaaa tcgttgaagg ctttgctgtc ggttttgacg
      961 gcatcgaggt tacctgcggc gcgaatcatc atctgaccgg cacggcgggc ggctttaaag
     1021 gctgtattca aaaacggatt catcagattt ccttaagggt ggcataccgc cggttcggac
     1081 gtgcagtcct tcggagcggc aaaatcggag tttatctggt tggggcaaac cctgccaaat
     1141 cgggtaaaat accgcctgac gcgtgtctgc ttcaggcgca acgttaaatt tccgacgttg
     1201 ttaaagaaca gttcaaacgg catttcaccg tccgaacgaa aaagacggcg cattataccc
     1261 tattccattc cgaccgaaaa ccgaacatga ctgctctcaa acccgccctg cccgactacc
     1321 tcggcaacat ccgcatcatc ctcacacgca ccagccaccc cgccaacatc ggctctgccg
     1381 cacgcgcgat gaaaacgatg ggtctgcaca ggctgaccat cgtcaccccc aatctgatgg
     1441 caacgccgat gacggaaaat ccgcccgttt ttaatcccga tgacgtgcaa agttttgcac
     1501 tgcctgaaga aagtttcatc ctcgcttccg gcgcggcgga cgtattgcac aacgccgaaa
     1561 tcgtcgccac actggacgaa gcccttgccg acaccaccat cgcctgcgcc ctgaccagcc
     1621 gccgccgcga aatcaccgcg ccgctgcaaa ccccgcgcga tttggtgccc gaattactgc
     1681 aggccgccaa ccgcggcgag aaagtggcgc tggtcttcgg caacgagact ttcggcttga
     1741 gcatcgaaga agtccgagcg tgcaaccgac tgatgaccat caacggcaac cccgactatt
     1801 tctcgctcaa cctcgcccaa gccgtgcagg tcgtgtgcta cgaaattttc agccaaaccg
     1861 attcgcccat gacccatctt caacaggaag accacgccgc aacccacgag caaatcaaag
     1921 gcatgctcgc ccacatggaa agcgtgatgg acgacatcgg ctttttcaac cgccgcaacg
     1981 gcgagcgtct gatgcgccgt atgcagagcc tgttcggacg cgccaacacg caaaccgaag
     2041 acatcgacat cctgcgcggt tttttcaata ccgtcagcca ccgtatccat aaaaaagact
     2101 gattaaggcc gtctgaaaac attacaagct ttttcagacg gcatgactga tattcggata
     2161 agcatgaatt acgccctaga cgcattatgg tggaaactca ccagccaacc cgtccgcgac
     2221 ctcgcctcgc tgctgactgc gccgcctttg tggcaaagcg gttgcgaatt gagcgtgcgc
     2281 gaactactgg gggaacgcgg tttccgctac cttttagcat tggatgccga tcccgcgccg
     2341 ctgacggatt accttgcaca acgcgccccg ttcgaccacc gtctcggcat ttatgccgaa
     2401 gagctgctgg ctttttggtt taccaacgca ccgcacacca aactgcacgc atataatctg
     2461 accgtttttt cagacggcca aactttaggc gccgcggatt ttgtcgtttc cctcaaccaa
     2521 cagccctatc atatcgagct ggcgtgtaaa tactacggcg gagaccaagt gcagaacctg
     2581 cgcggtctca atcccaaaga cacgctgacg gacaaagccg ccaaattggt gcagcaattc
     2641 cagctggtgc atacgtcgca aggcaaagca accttagccg cacaagacct gccggaaaat
     2701 ccacttcctg cttccatcgt gcgcggcatc ggattttttc cacaaggttt ccatgctttt
     2761 gagccgccgc ttaatccata cggctggcgc ggcatctata ttcaagattg ggcggaatac
     2821 gggtttgaac gccaagaagc gcgctatcac ctgctcgacc gcatggtcta cctcgcgcct
     2881 gcgcgtgtcg ccgaaaccga aacattaaac gcaaccgaaa tccgccgcat cgaccaaggc
     2941 ttgattgccg ttttggaatg tcggccggac ggcttttggc acgaaatcga acgtattatg
     3001 aaggccgtct gaaacccttt accaacttta agagaaccca ttacattatg aacgccgcac
     3061 aactcgacca taccgccaaa gttttggctg aaatgctgac tttcaaacag cctgccgatg
     3121 ccgtcctctc cgcctatttc cgcaaacaca aaaagctcgg ccgccaagac cgccacgaaa
     3181 tcgccgaaac cgcctttgcc gcgttgcgac actatcaaaa aatcagtacc gccctgcgcc
     3241 gtccgcacgc gcagccgcgc aaagccgcac tcgccgcgct ggttctcggc agaagtacca
     3301 acatcagcca aatcaaagac ctgcttgatg aagaagaaac agagtttttc ggcaatttga
     3361 aagcccgtaa aaccgagttt tcagacggcc tgaataccgc cgcagaattg ccgcaatggc
     3421 tggtggaaca actgcaacag cattggagcg aagaagaaat cctcgctttc ggccgcagca
     3481 tcaaccaagc tgcgccgctc gacatccgcg tcaatacctt gaaaggcaaa cgcgacaaag
     3541 tattgccatt gttgcaagcc gaaagccccg atgcagaggc aacgccttat tctccttggg
     3601 gcatccgcct gaaaaacaaa atcgcgctta acaaacacga actgtttttg gacggcacgc
     3661 tggaagtcca aggcgaaggc agccagctgc ttgccttatt ggtgggcgca aaacgcggcg
     3721 aaatcattgt ggatttctgt gccggagccg gcggtaaaac cttggctgtc ggcgcccaaa
     3781 tggccaataa aggcagaatc tacgccttcg acatcgccga aaaacgcctt gccaacctca
     3841 aaccgcgcat gacccgtgcc ggactgacca acatccaccc cgaacgtatc ggcagcgaac
     3901 acgatacccg tatcgcacga ctggcaggca aagccgaccg cgtgttggtc gatgcgccct
     3961 gctccggctt aggcacactg cgccgcaatc ccgacctcaa ataccgccaa tccgccgaga
     4021 ccgtcgccaa acttttggaa cagcaacaca gtatcctcga tgccgcctcc aaactggtga
     4081 aaccgcaagg ctgtttggtt tacgccacct gcagcgtgtt gccggaagaa aacgaactgc
     4141 aaatcaaacg tttcctgtcc gaacatcccg aatttgaacc cgtcaactgc gccgaactgc
     4201 tgcaaaacct gaaggtcgat ttggataccg gcaaatacct gcgcctcgat tccgcccgac
     4261 accaaaccga cggcttcttc gccgccgtat tgcaacgcaa ataaaccggt ttgaacaaaa
     4321 tgccgtcaga acccttttca aagcgttcgg acggcatttc atcaattaaa aatatcaaaa
     4381 accgcagatt caaccgattc aacaccaaag gcagaatcac gcgcctcatc ggattttata
     4441 cgcgcctgcc tgcaattttc tttttctttg ccaaaatata cggtagcata accatattcc
     4501 aatgatattt ttgggaatct gttttacccc aatatataaa gcaccatatt aaggcggagt
     4561 gtcttccctg ctttgacccg aacccggaaa agacaccgcc cgaaccaatc ccgattctgc
     4621 ccgggcagtc agccatcaag gaaaccctaa tgaactttgc tttatccgtc atcacattta
     4681 ccctcgcctc tttcctgccc gtcccgcctg ccggagccgc cgtctttact tggaaagacg
     4741 gcggcggcaa cagctattcg gatgtgccga aacagcttca tcccgaccag agccaaatcc
     4801 tcaacctgcg gacgctccaa accaaaccgg cggtcaagcc caaacctgcc gtcgatacga
     4861 atgcggacag tgcgaaggaa aacgaaaagg atatcgccga gaaaaacggg cagcttgagg
     4921 aagaaaagaa aaaaattgcc gaaaccgaac ggcagaacaa agaagaaaac tgccggattt
     4981 caaaaatgaa cctgaaggcg gtgggaaact caaatgcgaa aaacaaggat gatttgatcc
     5041 gtaaatacaa taacgccgta aacaaatact gccgttaatc ggctctagcg caaacccgat
     5101 gccgcctgaa gcggcacggg gtttgtttat ttctgccagt aggttttgac gttgacgaac
     5161 tcgtacaacc cgaattcgga cagttcgcgc ccgtaaccgg aatctttgac tccgccgaaa
     5221 ggcaggcgca aatcgctgct ggtatggcgg ttgataaaca ccgatccggc ctgtattttt
     5281 tcggcaaacc gccaggcgcg ttcagtatcg gcggtataaa tgcaggcacc gagcccgaac
     5341 ggggaatcat tggcaaggca gatggcgtgt tcttcgtttt cggcgcgcaa aatcagggcg
     5401 acggggccga atacttcttc tctccagacg cggcaggcag gatttaccct gtctaaaacc
     5461 gtcgcagggt aaaaccaccc cgacccctgt ggaactttgc ctccggtcag gcacaccgcg
     5521 ccgtttgaaa cggcatcttc aacctgcccg tgaacccggt cccgcaaatc ttcgcggtgc
     5581 agcggtgcaa gcgtggtatc gggatgtttc gggtcgccca ttttcaattt ggcgcattcg
     5641 gcgagaaaca gctgaataaa gcggtcggct gcggcttcgg ttacgatgat gcgcttggcg
     5701 gcgttacacg attgccccgc atcgcggaaa cgggaataac aggcttctgc ggcggcgcgt
     5761 tccaaatcgg catcgggcat cacgataaag gcgttgctgc cgccgagctc caacacggtt
     5821 ttcttgaggt ttgcgcccgc atgtgccgca agaatgcgcc ccgtatgcgt cgaaccggta
     5881 aacgccatcg catcggtatc ttcgactgcc ttgagcgtgt ccgcctcatc cagccacgcg
     5941 cctgccagag gaataccgtc tgaagccaaa tcgaacaacg cccgactgac gcgtgccacg
     6001 ctgggcgcgg gtttgacggc gcacgcgttg cccgcgcaca tagcgggaac ggcgaaacgc
     6061 aatacctgcc agacgggata gttccaaggc atgacggcaa acaccacgcc caaaggctcg
     6121 aagcgcacct gactcaaact cgcctgcgtc gcaatggttt tgtgggcgag cagttcgggg
     6181 gcgaggcggg cgtaatagcg tatcagttcg atagacttga cgatttcggc acggcattcg
     6241 tgcaggcagc gtccgacttc ttcgcacacc atttccgcaa aacgctcttt ttccgcctcc
     6301 aaacggccgg caaatttttg cagacgcgcg gcacgttcgg ttacgcccaa ttgcgcgaac
     6361 gccctgccgt gcattttcaa atccgccagc cgccgttcaa actccgcata atcttgatcg
     6421 gggcggcggt aaagcgtttc gcccgtaaat acattgacac tgtgaaacat cgaatcaacc
     6481 tgccagttgc gggaatatcg ttttcagtcc cgacacaata atctccaccg ataccgccgc
     6541 cagcatcata cccataatgc ggtttaaaat cgtcagcccc gtcgcgccca gcaggcggct
     6601 gaccttcccg gcaacgatta aaatggcata acaaatcgca ctgaccacca aaccggccgc
     6661 gataatcagc gcgatatcgc tgtacgtttt ggctgccgaa gcgtaaataa tcacggtcga
     6721 aataccgccc ggaccgatgg tgatcggtat ggcgatgggc acgacggcaa tcgcccctgc
     6781 attgcgggcg gggcgcgctt gccccgtttc cggctgcgcg ccgagattct gcttggcggg
     6841 attgtcgttg ccgttcatca tcgaaatggc gatcagcagc accaaaatcc cgccgccgac
     6901 ctgaaacgaa ccgacgctga tgcccaaaac cttcaatagc gcaccgccga tcagcgcaaa
     6961 taccgcaatc acggcaaaca cggcgacggc ggccgtccgc gcgaccttcc tgcgctcctt
     7021 cgtgctgtgt ccgttggtca ggtcaaggta aagcgacaac gcgctaaacg gattgatcag
     7081 caccaaaaaa gccacaatca gcttgccgat ttccataccc aatcccatta tttcccctct
     7141 ttcaaacccg tgcggcaggc atccgatgct gcaaattgcc gccgcaacgg atttttccgt
     7201 tataattaaa aatccaagca atacgcccca tcatacccga acgacggtat ctttaccatc
     7261 agacaaggat gcttttcatg gcactgacac ttgccgacgt agacaaaatc gcccgactct
     7321 cccgactgca gctgactgcg gaagaaaaag aaaaatcgct tcaagaatta aacgacattt
     7381 tcactatggt cgaacagatg cagaacatca acacagacgg catcgagccg atggcgcacc
     7441 cgcacgaggt cgccctccgc ctgcgtgaag acgaagtaac cgaaaccgac cgcgccgccg
     7501 aataccaagc cgttgcgccg gaagtacgca accgcctgta catcgtaccg caagttatcg
     7561 aagaataatc cgaatacgct tcagacggca tcagcaatac cgcccgaagc cctttaagga
     7621 tggaagattt atgacccaat acacattgaa acaggcaggc agcctgttgc agtccaaaca
     7681 gatttccgcc gtcgaactgg caagcgcata ccttgccgcc atcgctgaaa aaaatcccgc
     7741 cctcaacggc tatatcacca tcgaccaaga taaaaccctt gcagaagccc gtgccgccga
     7801 cgaacgcatc gcgcagggca acgcttcggc acttaccggc gttcccgtcg cctacaaaga
     7861 cattttctgc caaaccggct ggtgcagcgc gtgcgcttcc aaaatgctcg acaacttcat
     7921 ctccccctac accgccaccg tcgtccaaaa cctgctcgac gaaggtatgg taacgctcgg
     7981 ccgcaccaac atggacgagt tcgctatggg ttcgaccaat gaaaactcat tctacggcgc
     8041 ggccaaaaac ccgtggaatc cggaacacgt acccggcggt tcgtcaggcg gttccgccgc
     8101 cgtcgttgcc gcgcgcctcg cccctgccgc gctcggttcg gacacgggcg gctctatccg
     8161 ccaacccgca tcgcactgcg gcattaccgg catcaaacct acctacggca cggtttcccg
     8221 cttcggtatg gtcgcctacg cctccagctt cgatcaagcc ggcccgatgg cgcaaaccgc
     8281 cgaagactgc gcgattctgt tgaatgcaat ggcaggtttc gaccccaaag actccaccag
     8341 cttcgaacgc gaaaaagaag actacacccg cgatttggac aaaccgctca aaggcgtgaa
     8401 aatcggcctg cccaaagaat acttcagcga aggcaacagc accgatgttc agacggcatt
     8461 gcaaaacacc attgatttgc tgaaagcaca aggcgcggaa ctggtcgaag tttccctgcc
     8521 gcaaaccaag ctgtccatcc ccgcctacta cgtcctcgcc tccgcagaag ccggcaccaa
     8581 cctttcacgt tacgacggcg tacgttacgg acaccgtgcc gcccaattcg gcgatttgga
     8641 agaaatgtac ggcaaaaccc gcgccgaagg ttttggcagc gaagtcaaac gccgcatcat
     8701 gatcggcact tacgtactgt cgcacggcta ctacgatgcc tattatctca aagcccaaaa
     8761 actgcgccgc ctcgttgccg atgactttca gacggcattt gcacggtgcg acctcatcct
     8821 cgcgccgacc gcaccctccg ccgcacccaa aatcggagcg gatacttctc cggttgaaac
     8881 ctacttgagc gacatttaca ccatcgccgt caacctcgcc ggactgcccg cattgaccct
     8941 gcccgcaggc ttcagcggcg gcggactgcc cgtcggtgta cagcttgtcg gcaactactt
     9001 cgccgaagcc aaaatcctcg gtgcggcgca tcaaatccaa ctcaacagcg attggcacgg
     9061 caaacgaccc gaatgaagca gaaccgcacc tttaccttcc ccgattttcg caccgtttac
     9121 agctatgcgc ctttatatcg gctgcaacat ttaaaataca cattgcgaaa atttttcgga
     9181 aaaaaagaaa tttacgcctt cgagcagttt gtcaacgcct cccctatccg tcaggggctg
     9241 ttcctccact gcccgcagga tgcctatccg ctgctgcgcg aatttgttga caggcgtttt
     9301 aactgcaaac gccgtttaga tgcgatgacg gcagattttc tcatggcgga aaaactcttc
     9361 ggcacagaca ttctgtgcca aatggaagac tgccgcttcc atctggtctt ggcgcacctt
     9421 tcagacggca tcagcttgtg gctcaaccgc aacgacaact gcgtcgaaga aggcgcgtgg
     9481 tctttatctt tgcgcgatga agcaggcaac cggctgtata tggcgacctt cgcctttgtc
     9541 ggcacacacc tgctgacagc ctccgtacaa gggccgtcag gtgaagaagc caaagacacc
     9601 gtccgccgca taaccaaaca acttcacggc ttacgtcccc aacaactgat ggtaaccgca
     9661 ctgcaatatt tcgccgccgc attaaaatta gacggcgcga taggcattgc acaaaaacat
     9721 caagtcaaat tacgctggaa acttaaaaag cgcgtcaaaa tgaattacga cgcattctgg
     9781 caggaatacg gcgcaagttt ggaacgggac ggctactggc atcttcccca aacccccgcc
     9841 cgcaaagacc ttgccgacat cgaaagcaaa aagcgttcga tgtaccgcaa gcgttatgaa
     9901 atgctggacg atatggttgc aaagatgaag gacagtctga aaacagaagc acgcggcatt
     9961 tcagacggca tccaaacgga aaaaccgctc cgccggacag tctgatgcga agactatcga
    10021 attgatattt tagagaaaga acctcctatg acttgggaaa ctgtaatcgg cttggaaatc
    10081 cacgtccaat tgaacaccaa atccaaaatc ttcagcggtg catcgaccgc attcggcgca
    10141 gaacccaacg cgcacgccag cgtagtggaa tgcgcgctgc cgggcgtact gccggtaatg
    10201 aaccgcgaag tcgttgaaaa agccatcaaa ttaggtttgg ctttggatgc aaaaatcaac
    10261 cggaaaaacg tgttcgaccg caaaaactac ttctatcccg acttgccgaa aggctatcaa
    10321 atcagccagt tggatttgcc gattgtcgaa cacggcaaat tggaaatcgt ggtcggcggc
    10381 gatgtgaaaa ccatcaacgt aacccgcgcc catatggaag aagacgcagg caaatccgtg
    10441 cacgagggct tgagcggcgc gaccggcatc gacctgaacc gcgccggcac gccgctgttg
    10501 gaagtggtat ccgaacccga aatgcgctcc gccgccgaag ccgttgccta cgccaaagcc
    10561 ttacacagct tggtaacctg gctggacatt tgcgacggca atatggcgga aggttcgttc
    10621 cgcatcgatg ccaacgtatc cgttcgcccg aaaggccaag cggaattcgg cacgcgccgc
    10681 gaaattaaaa acctcaattc cttccgtttc ttggatcagg cgattaatta cgaagccgaa
    10741 gcgcaaatcg agattttgga agacggcggc acagttcagc aggcgacgat gctgttcgac
    10801 ccggaaaaag gcgaaacccg cgtgatgcgc ctgaaagaag acgcgcacga ctacggctac
    10861 ttccccgatc ccgatttgct gcccgtcatc atttcagatg cccaaatgca aaaagccaaa
    10921 gcagaaatgc ccgagctgcc gaaagaaatg gcggcgcgtt tcgtggcgga ttacggcgtg
    10981 tccgaatacg acgcgcgcct gctcaccgcc agccgcgtgc aggctgccta ttttgaagaa
    11041 gccgccaaag agagcggaca aggcaagccg actgccaact ggatgaacgg cgaacttgcc
    11101 gctacgctga acaaagaagg catggaactt gccgacagcc cgattaccgc cccacgcctt
    11161 gccgcgctgg tgggcaaaat tgccgatggc acattaagcg gcaaactggc gaaaaaagcc
    11221 tttgaagcca tgtgggcgga accggaaacc agcattgccg aaatcatcga aaaacacagt
    11281 ttgcaacaga tgaccgatac cggcgcggtt gaagcgatgg tggacgaagt gctggcaaac
    11341 aacgccaaag ccgtggaaca gtttaaatcc ggcaacgaaa aagccctgaa tgcgattgtg
    11401 gggcaagtga tgaagaccag caaaggcaaa gccaatcccg cgcaggtgca ggagttgatt
    11461 aaagccaaac ttgcctgata agcaagatgc cgtctgaaac ccaaatccga cgttcagacg
    11521 gcattttcac agtctgacca cattaaaaca gcataaaaaa caatattcca cgtttcaaca
    11581 cacaggacga cacataaaat gtcgccctgt gtgttgccct gatttgaaag gggttacgcc
    11641 cctcccaaat aaagtctgat cctatcggcc taaagggtgg ggtttcaacc gaaaaggaaa
    11701 tacgatgaac cacaccgtta ccctgcccga ccaaaccacc tttgccgccg gcgacggcga
    11761 aaccgttttg tccgctgccg cccgtcaaaa cctcaatctg ccccattcct gcaaaaacgg
    11821 tgtctgcgga caatgcaaag ccgaactggt cagcggcgat attcaaatgg gcggacactc
    11881 ggaacaggct ttatccgaag cagaaaaagc gcaaggcaag attttgatgt gccgcaccac
    11941 cgcgcaaagc gatatcaaca tcaacatccc cggctgcaaa gccgatgccc tacccgtccg
    12001 caccctgccc gcacgcatcg aaagtatggt tttcaaacac gatgtcgccc tcctgaaact
    12061 tgccctgccc aaagccccgc cgtttgcctt ctacgccggg caatacattg atttactgct
    12121 gccgggcaac gtcagccgca gctactccat cgccaattcg cccgaccaag aaggcatttt
    12181 ggaactacac atccgcaggc gcgaaaacgg tgtctgctcg gaaatgattt tcggcagcga
    12241 acccaaagtc aaagaaaaag gcatcgtccg cgttaaaggc ccgctcggtt cgtttacctt
    12301 gcaggaagac agcggcaaac ccgtcatcct gccggcaacc gacacaggct acgcccccat
    12361 ccgcagcatc ctgctcgacc ttatccgcca aaacagcagc cgcgctgccc atttctactg
    12421 gggcgcgcgt catcaagatg atttgtatgc cctcgaagaa gcacaagggt tggcatgccg
    12481 tctgaaaaac acctgcttca cccccgtatt gtcccgtccc ggagagggcc ggcagggaag
    12541 aaaaggccac gtacaagaca tcgcggcaca agaccacccc gacctgtcgg aatacgaagt
    12601 gtttgcctgc ggctctccgg ctatgaccga acaagcaaaa aatctgtttg tgcaacagca
    12661 taagctgccg gaaaacttgt ttttctccga cgcattcacg ccgtccgcat cataattcct
    12721 cggtataaag aggattcggg ctttccgttc ggaacacaaa aaacttcccg tccgtatttt
    12781 ccccgtgaaa aaatgccgtc tgaaacctga ttccggtttt cagacggcat atgttttttc
    12841 ctgttcaagg cgacaaccgc tcgcgtatcc agccgccatc cagcaaacgg tattggatgc
    12901 ggtcgtgcag cctgctcggc ctgccctgcc aaaactcaat caaatcggga atcacaatat
    12961 agccgcccca atgcggcgga cgcggcacgt gcaaaggatg tttgagtccg accgccgccg
    13021 cctttgccac caataccgcc ttgttcggaa tcacctcgct ctgcgcgctt gcccacgcac
    13081 ccaaacggct ctgatacggg cgactctcaa aatattcgtc cgacaacttc tccgccagcc
    13141 ttccgacacg cccttccacg cgcacctgac gctccagctc cggccaaaaa aacgtcatcg
    13201 ccgcaaacgg attgagttcc agcgaacgcc ccttgcggct gtggtaattc gtaaagaaaa
    13261 caaaaccttc tgaattaacc tccttcaaaa gcaccatacg gctgttgggc ctcccgcgcc
    13321 cgtcgaccgc cgccacattg accgccgtcg gctcgttgac ctcggcgcgt accgcctcgt
    13381 ccaaccaccg ctcgaactgc tcgatcggat tatcggcgca atcggcttcc gacaattccc
    13441 gtttgctgta atcttcccga atattgtgca aatccattta ccgctcctct tatcatttga
    13501 aagattctac tcccgcacgc aaaccgattt caaccgttgc acaaactttg tccccaaagc
    13561 cgcagcgacg atttcatccg caaaaccgcc gcatcaggta caatatcgaa ccgtccgacc
    13621 gaggacggca ttttatcaac ccgtcctgcc gcacacgccg cagaagaacc gccttatcag
    13681 gcgagttagg aaaaatgatg tccaagcaac ccaccagcaa acgccaatgg cgcgacggcg
    13741 cagccccgtc tgccaagaaa accgccaaac ctttcaaaag caaagcccgt cccaaagacg
    13801 aaacgcgcaa aaccgccgcc caagcttacg gacaaaaagc ttcagacggc atcaagctgc
    13861 aaaacgcccc caaacagcgc gccgccaaag ccaaaaaact cgtcgtccgc aatcccaacc
    13921 aaaaaattat ggaacacgcg cgcgatttga aagaacgccg cagcgacctg tcgcgtatgg
    13981 aacccgaacg cctgcaaaaa gtgcttgcag cctccggcgt cggctcgcgc cgcgaaatgg
    14041 aagaatggat caacaacggc tgggtaacgg tcaacggcaa aaccgcgcaa ctgggcgaca
    14101 aagttacccc cgacgaccac gttaccgtca aaggcagcat catcaaactc aaatgggccg
    14161 accgcctgcc gcgcatcatc ctgtattaca aacaagaagg cgaaatcgtt tcccgcgacg
    14221 acccgcaagg ccgcgtcagc atattcgacc gcctgccgca ggccgccagc agccgctggg
    14281 tcgccatcgg acgcttggac atcaacacca gcggacttct gattctcact acctccggcg
    14341 aactcgtcca acgtttcgcc caccccagct tcgaagtcga acgcgaatac gccgtgcgcg
    14401 tactgggcgg gctgactacc gaacaaatgc gcgtcctcac cgaagaaggc gtgatgctcg
    14461 aagacggctt ggcaaaagtc gaacgcatcc gcgaacaagg cggcgaaggc gcaaacaaat
    14521 ggtacaacat cgtgattaaa gaaggccgca accgcgaagt gcgccgcata ttcgaaagcc
    14581 aaggcctcac agtcagccgc ctcgtgcgcg tcgccttcgg ccccatcgga ctgcccaacc
    14641 gcctcaaacg cggacagttc tacgaactca accccgccga agtcgccaac atcctcaaat
    14701 gggcggatat gctgctgccg ggcgaacgcc gccgcaaaaa agcctgaaag taaaaatgcc
    14761 gtctgaaaca cctgctgttt cagacggcat tttattcggg cgttttcagg agaaaaggtc
    14821 gagtgctttg acaaagacca tcaccacgcc gtaggcgagc ggtatgacgg ccaatgccca
    14881 acgccaccag accgatatgc cgtcgccgga aactttttcg cccacaaggt aagcgtcgga
    14941 tatggcggtt tcgtcgtcgg ggttgccgct gcgtgcggcg gttttgatgt ctttttcgtg
    15001 gtgtttttcg tgtacggatt tgacggcgag gttgcacaac agtccgacaa tcaacaatcc
    15061 cgccataatg tacatggtta cgctgtatgc ctgtgccgcc ggtatgccgc tgtcgatttg
    15121 gctttggcgt atgtaattga ccagtaccgg gccgatgacg gcggcggttg accaggccag
    15181 caggatgcgc ccgtgaatcg cgccgacctg ataggtgccg aacaggtctt tcaggtaggc
    15241 gggaatggcg gcaaatccgc cgccgtacat ggaaataatc acgcagaagc cgatgatgaa
    15301 cagggctttg ctgccgcctt cgccgatgga gggaacggcg aaatacagca gcgaaccgag
    15361 tacgaagaag atggtgtagg tgtttttgcg tccgattttg tcggaaacgc tcgaccacaa
    15421 aaagcgtccg cccatattaa acaggctcag gaggctgacg aagcctgccg ccgcacctgc
    15481 gccgactgcc gactgcctgc ctgcggaggt ttcggaaaag agttcctgaa tcatcacgga
    15541 tgcctggccc aatacgccga tgccggcggt tacgttcagg cacaataccc agaacaacag
    15601 ccaaaactgc ggcgttttca tggcttggga cacgttgaca tgattgctgc tgaccggctt
    15661 gttttgcgtt ttcggcacgg tatagccttc aggtttccag ccgtcggcag gtacgcggat
    15721 ggtaaacgcg ccgaacatca tcagtgcgag gtaaaacagg cccaatacgg caaaggtttc
    15781 ggcaaccccg accgaagcgg cgtttgaaaa ggcgttcatc agtgatacgg aaagcggcga
    15841 ggccagcatt gcgccgccgc cgaaacccat aatcgccaaa ccggtcgcca tacccggctt
    15901 gtcgggaaac catttcatca gtgtggaaac cggcccgacg tagcccaaac ccaagcctac
    15961 gccgccgatg acgccgttgc ccaaatagag caggaagagg ttgtgcgtac gcacgccgaa
    16021 tgcggatacg aagaagccca ggctgaaaca gcaggcggcg gcaaatatgg ctttgcgcgg
    16081 ccctacccgt tccatccatg tgccgaacag ggcggccgac gcgcccagca tcgcgagtgc
    16141 gatactgaaa atccaaccta cggtcgtcag cttccaatct ccggccgccg attcggttat
    16201 gccgataagt ttggtcagcg gcgcgttgaa tacggaatag gcgtaaatct gcccgatggc
    16261 aaggtgcacc gccaatgctg cgggcggtac gagccaacgg ttgaaacccg gcttggcaat
    16321 gcttgcctca cggtctaaaa acttcataac atcctctttc tgtcagttga aaaataaaat
    16381 ttcatttgcc caatggaaac ttattaaaaa ttataaaaaa atatcgggtt gggtttttat
    16441 ccgccccaag atgcgccgtc tgaaacattt cgggtgtgcg gagaggtttc tgttttttcc
    16501 gacaaattcc tgcggctttc cgcttccgga ttcccgcttt ttagggactg gattcccgtt
    16561 ttcgcgggaa tgacggcggc aggttgctgt ttttttgttt ataaatttct gtaactttga
    16621 atttcagcca tcttcaataa attatcgaaa cgtatcaatc gtcattcccg cgcaggcggg
    16681 aatccgtttt ttgagtttca gtcatttctg ataaaccccg gcggctttcc gcttccggat
    16741 tcccgctttt gcaggaatga cgaattaaag attatcttaa ggtcaaggga ctggattccc
    16801 gctttcgcgg gaatgacggc ggagggggag cggtttttcc gattgggttt aaatgcaatc
    16861 gaacaaatcc tgctgccctt gttctttgct tacgcgcacg tcggtttcgc cgtcggagaa
    16921 ggtaatgtgc agcttctgcc cctgcttcaa aacatcggcg ttacggatga cttgtccgcg
    16981 tgtatttttg acaacggaga agccgcgttc cagaatgtgc tgcggcgaaa cggcttcgag
    17041 caatgcggct tgggcggtca ggctttgacg gcgacgggcg agcagccggc tgaaggcggc
    17101 gggcaatgcc gtctgaaggc ggacgatatc ttgccggtaa acggaaacat caggacggca
    17161 atgttgcagg gcttgggttt ggcgttcaaa acgggcggtg tgggcgcgga ggttttgcgt
    17221 catcgagtaa gacagcgttt gcgccagctt gccgattgaa gcgcgctgct cgtcaagttt
    17281 ttggcgcggg tggcggattt gccgcgcgag ccagtcgagt ttttggctgg catcgaaata
    17341 gcgttgctcc aaaacggttt tcagacggcc ttgggcttgg acgaggcggt gcagtgattc
    17401 ttggcggttg gggctgacca gttccgccgc gccggtcggc gtgggcgcgc gcacgtcggc
    17461 aacgaaatcg gcgagcgtga aatcggtttc gtgtcccacg ccgctgacga cgggaatcgt
    17521 gcaggcttcg atggcgcgca cgaccggttc ttcgttaaac gcccgcaagt cttcgatgct
    17581 gccgccgccg cgacagacaa tcagcacatc gcattcggcg cgttgcgatg cggttttaat
    17641 cgcttgggca atctgaaacc cgctgcccgc gccctgaacg gcggccggat aaacgatgac
    17701 ggggatttcg ggcgcgcggc gtttcaaggt ggttacgaca tcgcgcaaag ccgccgccgc
    17761 cagactggta acgatgccga tacattgcgg acggacgggc aaaggttttt tgcgttccgc
    17821 cgcaaacgcg ccttccgcct gcaactgcgc cttcaaccgc tcgtaggctt cgtaaagctg
    17881 ccccaaacct ttgagccgta cttcgtttac ggtaatctga aattcgcccc gcgcctcgta
    17941 aatactgatt ttgcccgcca cttcgatatg gtcgccttct ttcaaaggtt gcgccaaacg
    18001 cgccgccgca cccttgaaca tcgcgcaacg cacctgcgcg cggctgtctt tgagtgagaa
    18061 ataataatgc ccgctggcgg cgcgggtcag attggacact tcgcccgcaa tccacaggcc
    18121 ggcaaggtgg ttttccaaaa tacttttggc aaatgcgttc aactcggaaa cggacaacac
    18181 gtcagaatga aaaaaatcag acatcgaatc aatcaaataa taaaaaatat gaatatgttt
    18241 tgaagcccaa ggcggcatcg tgccgcctaa attgtcaaca atattataac acgcgccatc
    18301 ttgccgccag ccttttccta tatgactttt ttaagcgggg aatgggaaaa atattcatca
    18361 acccgccctc aatctattca aattgcaccg ccggcaggct atgatgcgga tattttcgac
    18421 aggaggaaaa atggatacgc aggcagttat cacacatatt gtccgatggt tggacgaata
    18481 cgccgctcag gcaaatgcaa aaggattcgt cgtaggcgtt tccggcggca tcgattccgc
    18541 cgtcgtctcc acgctcgccg cccgcaccgg ccgcaccacg ctgcttctgg atatgccgat
    18601 acgccaacac cccggccagc tcgagcgggc aaggcggcac atccgcaatc tgcaagggca
    18661 atatgccaat gtaagcgcgc aaacggccaa tctgaccgac accttccaaa cctttgagca
    18721 aaccgtcggc gttcatcaga cggcatttgc caatcagccg ctttcgctcg ccaacgccag
    18781 aagccgcctg cgtatgctga ccctgtacta ctacgggcag atacacggac tgctggttac
    18841 ggggacaggt aataaggttg aagatttcgg cgtgggtttt tttaccaaat acggcgacgg
    18901 cggcgtggac atcagcccga ttgccgacct gaccaaaacg caggtttacc ggcttgccga
    18961 agcattgggc gtggacgagg cgattcaaaa agcgccgccg accgacggcc tgtgggatac
    19021 ggaacgcacc gacgaggaac agatgggcgc aagctatccc gaactggagt gggcaatggg
    19081 cgtgtacggc acgcgcaaac ccgaagattt tgaaggacgg cggcgcgaag ttctggaaat
    19141 ctatacgcgg cttcaccgcg cgatgcagca caaaatcaac ccgattcctg tgtgccgcat
    19201 tccgcccgaa ttgctgggct gaaacatgaa aatgccgtct gaaacggaaa aaccgtattt
    19261 cagacggcat tgaaatatcc gactcctatc ccttaagaat cgagtacgcg ggcaaacaga
    19321 atatcgtttt ccaaatgaat gtggtcgttc aaatcgtccg ccatttcttt tgccagcgcg
    19381 taaaggcgcg tccaacttcc gcaagcccct tcgggtgcgt ggaaattacc ggtcagctct
    19441 ttgagccgtg cgatggcgcg gtcgtgttct tcatgttcgt gcatcatcac gctgatgggc
    19501 atcgccgcac cgcgtccgac accctgatta atcatcggaa acagcatccg ctcttccttc
    19561 atcatatgca tcagcagctc gtcccgcata tcggcaagca gcccggcaat ttccgccgga
    19621 aaggtatcgg catggacttg ggccactttc tgcgccagcg gcaccaattc ttcaaattgt
    19681 gcgcggtgga cattgtggta gcgttgcagg atatgatcaa cggttgcgcc aaagggagca
    19741 gcttcccaaa cggaaaaatc agtcattgca gtgttccttt tatagggttt cgggtttggt
    19801 tttgaacatt catactttaa gaatctattt aaacggagca tacaccgccc gcgcgtttct
    19861 gtacagcctc aaaaacattc cttatacttt gataataaaa gtaattttca aaaataaaat
    19921 actgtccgaa ccgtttttta gaatttgcaa aggcgattgg ggcggtacag aaaaactatt
    19981 atcccgcccg cccacttgaa atttttatgc ccaagcccta tcctgcacgc tatcgtgcga
    20041 atcccaaccg aaaaggaaaa ataatgagca gcgaattaat cgtacacacc ggcgatgccg
    20101 ccttcgagca agatgttttg aaatccgacc tccccgtcct gctggacttt tgggctccgt
    20161 ggtgcggccc ctgcaaaatg attgccccga ttttggacga cattgccgcc gaatttgaag
    20221 gccgtctgaa agtagtcaaa atcaacatcg acgacaacga agccaccccg tcccgtttcg
    20281 gcgtgcgcgg cattccgacc ctgatggtgt tcaaaaacgg cgaagtcgtc gccaccaaag
    20341 tcggcgcatt ggcaaaaggt cagctgaccg cctttgtcga agcctccatc gcctgataaa
    20401 gcgcaatcga aaagccgccg gaagattccg gcggcttttt cgcaccctta agatttgtgg
    20461 cggatttccc aacaccggtg gatttttttg ttgcggaaat cttcgggaac ggattgtttg
    20521 gaaatgtctt tcacggcgta ttgttccgat accgaatcgt ccaaaacgaa gctgcgcagg
    20581 ttgttggaaa aatacaaaat gccgtctgaa gcgagcaggt tcaccgcgcc gtctatgagt
    20641 ttttgatggt cgcgctggat gtcgaggatg ccgggcattt tcttgctgtt ggaaaaactg
    20701 ggcgggtcca tcacaatgag gtcgaactgc ctgccttccc catatgccgt ctgaaggtat
    20761 tggaacacgt cggcgcggac gattttgtgc cgttccatat cgatgccgtt caattcaaaa
    20821 ttgcgcctcg cccaatcgag ataagtgttg gacaaatcga cagtttcgct ggatgccgcg
    20881 ccgccggtgg cggcatagac ggtgaagctg ccggtgtagg aaaacaggtt caaaaaacgt
    20941 ttgcccgccg ccgtttcgcc gacttttttg cgcgtgttgc ggtgatcgag gaagaagccg
    21001 gtgtcgaggt atttgtcgag gttgacccaa aacttgcggc cgttttcggc aatgacgaaa
    21061 tcgtcgcccg ccttgccggt tttctcgtac tgctgcaaac ctttttggcg ttcgcggcgt
    21121 ttgagacgga tttgttcggg ttcaaaaccg gtaacgaaag cgacggcttc caatacttcg
    21181 gcaagccacg cttcgtattc tccgggccgc atcagccagc cggtatcgta ttcctgaagg
    21241 tggattcgat cgccgtaaac atcggcggca aaagggaatt gggggatgtc gcggtcgtaa
    21301 atgcgccagg cttcgatgcc gttgcgtttc gcccatttca taaggtgttt gatgtttttg
    21361 cccaagcggt tggcaaacgg tgtgatgtcg gtcattgatt tcaggcggaa taaagtggaa
    21421 aacggaaatt ttactgtaat taacgcccga ttgcttgacc ttttcgggca aaccctatac
    21481 catccgtcgc ttatcttgtc atacgaagcc atcgccttcc aacctaaacc gcccttacgg
    21541 gcgcgtttct tctgttgctt tgattttgca aagcatatct gtgcaggttg ccgtcgatgt
    21601 aaaccacaag caagccgctt gcgacaaccc tgtaacttca cattccccgt atcgttaccc
    21661 ttccctgctt caggccgtct gaacctttcg gacgcgggcg ttgttgtctt ccaaggatag
    21721 ccatgtctat taaatttgcc gatttgaacc tcgataaaaa catcttgtcc gccgtcagca
    21781 gcgagggtta cgaaagcccg acgccgattc aggcgcaggc gattccgttt gctttggaag
    21841 gctgcgacat catggcttcg gcgcaaaccg gctccggaaa aaccgcagcc tttctgttac
    21901 cgactttgca gcgactgacc aaacgcagcg aaaaaccggg caaaggcccg cgcgcgctgg
    21961 tgttggcacc gacccgcgaa ctggcggcgc aagtcgagaa aaacgcgctg gcgtatgcca
    22021 aaaatatgcg ctggttccgc accgtcagca tcgtcggcgg cacatcgttc ggctaccaaa
    22081 cccgcgccct gagcaaaccg gtcgatttga ttgtcgccac tccgggccgt ctgacggacc
    22141 tgatgcaaag cggcaaagtt gattttgaac gtttggaagt gctgattttg gacgaagccg
    22201 accgtatgtt ggatatgggc tttatcgacg acatcgaaac catcgtggaa gccaccccgg
    22261 ccgaccgtca gactttattg ttctccgcca cttgggacgg cgcagtcggc aaactggcgc
    22321 gcaaactgac caaagactct gaaattatcg aagtcgaacg cgtggacggc caaggcaaaa
    22381 tcgaagagca gttgttgtac tgcgacgata tgcgccacaa aaaccgcctg ctcgaccaca
    22441 tcctgcgcga tgccaacatc gaccaatgtg tgattttcac gtccaccaaa gcgatgaccg
    22501 aagtcatcgc ggatgaattg tacgaaaaag gtttcgccgc caactgcctg cacggcgata
    22561 tgccgcaggg ctggcgcaac cgcacgctga cggatttgcg taaaggccgc tgcaaaattt
    22621 tggttgccac cgacgttgcc gcacgcggta ttgacgtacc gaccattacc cacgtcataa
    22681 actacgacct gccgaaacag gcggaagact acgtccaccg catcgggcgc accggccgcg
    22741 caggccgcac gggtattgcg attacgtttg ccgaagtgaa cgaatacgtc aaagtccaca
    22801 aaatcgaaaa atacattaac cgcaaactgc ccgaactgac catcgaaggc atggagccga
    22861 cccgcaaacg caaatccgca agcggcaagc cgaaaggcaa aggcggctgg ggcgatagga
    22921 aatccggcgg ccggcgcggc gatcataaac cgggcaaaga aggcttcggc ggcaaaacgc
    22981 gcggcgaagg tttcaagaaa gaaggcttta agaaagacag tttcaaaaaa accggcgaag
    23041 gcttcaaagg caaacgcaaa gccggcgact cttttgcagg caaaagcgaa cgccgttaca
    23101 aagaccgcta agccccaacc tgccgcgtaa accaatgccg tctgaaaccg atttcgagtt
    23161 tcagacggca tttttgtaat gtttcagcac cgcccggctt tgatacccaa aggattaatc
    23221 tgcaatatag tggattaaat ttaaaccggt accgcgttgc ctcgccttgc cgtactatct
    23281 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat aaaaaccctt
    23341 ttccgctttg gcaacgattg aaaatttccg taaattcaaa tatctagatt ccttcctgcg
    23401 tgggaatgac gcagaagggt ttcagatgca ggatggacat tcctgcctac ccaatctcgc
    23461 cctacggctt gacggttcga tatgatgccg tctgaaaacc caacggcggt atgacaatgc
    23521 cgccctgcca acgcacgtag atcagaattg ccatcccgac atcaaacgct tggaaacaaa
    23581 atgccgtctg aaaatcaaac ggcaacataa caatgcccct aacaaataca aaaatgccgt
    23641 ctgaaagccc ttcagacggc attggcgcgc cgggtttacc gccttctgcc gaaaccgcgc
    23701 atagcgggac ggcggtaatt gacgggcggg cgcgttgtcg ggcggtaacg ctgcgcctgc
    23761 gccgcctgtt gttttgcacg gaggctgcgc gtgttcaaat ccctgctggt gcgcgcattg
    23821 gggcgtgcgg actgatggta ggcagcacgc gcgcgacggg cgacggggct gtcttggttg
    23881 cccgcccgtg tgaatttgtt tgccagcgcg ttgccgataa acgcgcctgc cgccgcgcca
    23941 atcaggcttt gcagcagcca gcttcctgtc gattggtcgt aaatatactg ctgcccgtct
    24001 ttgccggtaa cgggctgacc gttgttgccg tttgcctgcg cctgggcagg aatggtgtct
    24061 ttgactgctt cgggagtcag ttggtaaacc gtatcgtctg cctgctgtgc gagctgctgt
    24121 tgcagggctt caatctgttt ctgctgccgt tcgagccgcg cctgcgtgtc gtcttggcag
    24181 gcggtgagtg cgaatgttgc gataagcgcg gaggcgatga tttttttcat gtgtgtcctg
    24241 ttcgggtgga aaatcggttg tattgtatcg ccgtcgggaa ttttggcaag cattctgccg
    24301 gcaaatcgtg atgtttacag gggtagggtg tgcagtttgc ggacaaatgc gaggctgttg
    24361 gcgacagggt tgcctttgtt ttcgacttcg tgttcggttt ctatggtcag caggcggcgg
    24421 tttttgtgtt tgttcaggct caattcggct tgtgcgggtg tgaaaaacag gcggtgtttt
    24481 tcggcaaagc ggcaggcgcg cttgtttgcg gttttcaaaa tctgaagcag cgatacgtcc
    24541 agatggaagc gtcgcacgcc caatacgaga atccgtgcgg caaaaaaatc ggcggcatcg
    24601 gtaaactcgg cggggctgct gcggctgaag tcgtgccgtt cggcggctat cagggcggca
    24661 acggtgcgga tttgcggaat catcgattcg ctgatgagcg tgtcgtcccg ccctgcatcg
    24721 agaagcatgg gggaaaaatc ctgcgcatca tcgacaataa tgctgcaagt gtgcaggttt
    24781 tcgttttgta cggcggtttg gggcattgca tccatggtca ttttcctgat tctgtcgtgt
    24841 gttgccgaat cgggcgacct gtgtgaaggt aacaaaaaag ccgccccgtt ttcgagcggc
    24901 ctgttttgcg tatgggatgg atttcaagca agcgcaaaaa gcaccgcacg tctgtgtggt
    24961 accaatagca gtaagcggtt gtaaacgttt tgccttgcat gatgaaatgc cgtccgaaga
    25021 taaaaatatt ggggagattc taaatcaaaa cgctgccgcg cctcaagcat tatatcgaaa
    25081 tttttttgtt tttttcagct atccggttga aaatatttag gtttattttt accactgccc
    25141 gatattgtcg gcaatttccc tttatccgcc ttgaaaaacg gtgcataatc ccgaacaaaa
    25201 ccgcaatcag gagcaattat gcaaaactat ctgaccccca atttcgcctt tgccccgatg
    25261 attcccgaac gcgcttcagg cagccgcgtt tgggatacgg aagggcgtga atatattgat
    25321 ttttcaggcg gcatcgccgt taacgctctg gggcactgcc accctgccct tgtcgatgct
    25381 ttaaacgcgc agatgcacaa gctgtggcac atttccaata tctatacgac gcggccggcg
    25441 caggaattgg cgcaaaaatt ggtgaaacac agttttgccg acaaggtttt tttctgcaac
    25501 tcgggcgcgg aagcgaatga ggcggcgtta aagctggcaa ggaaatatgc gcgcgaccgt
    25561 ttcggcggag gcaaaagcga aatcgtcgcc tgtatcaaca gtttccacgg acgcacgctg
    25621 tttaccgtgt ccgtcggcgg acagccgaaa tacagtaagg attatgcacc gctgccgcaa
    25681 ggcattacgc acgttccgtt caacgatatt gccgcgctgg aagctgccgt cggcgaacag
    25741 acctgcgcgg tcatcatcga gccgatacag ggcgaaagcg gcatcctgcc cgccactgcg
    25801 gaatatttgc aggcggcgcg ccgtctgtgc gaccggcaca atgcgttgtt gattttggac
    25861 gaagttcaaa ccgggatggg gcatacgggc aggctgtttg cctatgaaca ttacggcgtt
    25921 gttcccgata ttttgagttc ggcaaaagcc ttgggctgcg gctttccgat cggcgcgatg
    25981 ctggcgacag aaaagattgc cgccgccttc caaccgggca cgcacggctc gactttcggc
    26041 ggcaacccga tggcgtgtgc ggtcggcagc cgcgccttcg acatcatcaa cgcgccggaa
    26101 accttgcaca acgtccgcag tcaggggcag aaacttcaga cggcattgct ggatttgggc
    26161 aggaaaacag gcttgttctc acaggttcgc gggatggggc tgctgctcgg ctgcgcgttg
    26221 gacacgcctt atcgcggacg ctcatccgaa atcgccgcta cctccttgaa acacggcgtg
    26281 atgatcttag ttgcgggtgc ggacgtattg cgtttcgcgc cttcgctgct gttgaacgat
    26341 gaggatattg cggaaggttt gcggcgtttg gaacacgtgc tgacggaatt tgccgccgca
    26401 aaccgcccct aggcagaaag cagatgccgt ctgaagacgg gaaggcctca gacggcatca
    26461 gaaacaaaaa accgcttcgg aaacgtgatc caacgttccg aagcggtttt gtttgccatc
    26521 aggactcgaa caccaattcc ggttccctgc cctcttcgat gactgccttg ccgaccacca
    26581 cttttttcaa gccttgcaaa tcgggcaggc agtacatcgt atcgagcagg cagcgttcga
    26641 cgatggaacg caggccgcgc gcgccggttt tgcgttccat tgcctgccgc gcgatggaac
    26701 gcaatgcgcc ttcttcaaat tccaacccga cgttttctat gccgaacaag gcttgatact
    26761 gcttgaccaa agcgtttttc ggctcggtta aaatattaat cagcgcgtcc tcatccagtt
    26821 cttctaaagt tgcaatcacg ggcaaacgtc cgattaattc cggaatcagg ccgaatttga
    26881 ttaaatcttc cggttcgacg atgccgaaca gcttggtaat gccggcattt tcgtccttgc
    26941 tgtgaacgga cgcgccgaaa ccgataccac ctttctcggt gcgctggcga atcacttttt
    27001 ccaagcctgc aaacgcgccg ccgcagataa acaggatgtt ggcggtatca acgttgataa
    27061 attcctgatt cggatgcttg cgtccgcctt ggggcggaac gcttgccacc gtaccttcaa
    27121 tcagtttcag caaggcttgc tgcacgcctt cgccggacac gtcgcgcgtg atggacgggt
    27181 tgtcgctttt acgcgaaatt ttgtcaattt cgtcaatata gacaatgccg tgctgggctt
    27241 tttcgacatc gaaatcgcat ttgcccaaca gtttggtaat gatttgttcg acatcttcgc
    27301 cgacatagcc ggcttcggtc agcgtggtcg catccgccat cacgaacggc acgtccagtt
    27361 tgcgtgccaa agattgcgcc aacagcgttt tacccgatcc ggtcgggccg ataagcagga
    27421 tgttggattt cgacaattcg acaccgccgt cggctttcgg gtggcgcagg cgtttgtaat
    27481 ggttgtaaac cgcaaccgcc agcgcctttt tcgcctgccc ctgcccgatg acataatcgt
    27541 cgaggttggc aacgatttcg gcgggcgtgg gcagtttgcc ggattcttcc ggctcccctc
    27601 cggcattttc cggaggcgtg ccgccgttgt cgccttcgta caatatttcg aggcagtttg
    27661 agacgcattc gtcgcagata aaggcgtttt cgccctcaat caaatgtttg acgtgtgatt
    27721 tggattttcc gcaaaaggaa caggtacggt tttcgttgga catggctttc tttacaatgt
    27781 atgcgttgca gaaacggcat gcgccgttcg ggttgccaag tataataact atatccgtgc
    27841 ttatcaacgt attaccttaa aatcccgccg attcggctat aatacgccct ttcgcaaccg
    27901 ccccggcggc agaaatgccg tctgacacca aatctgagga tattcatgaa aaaaccccaa
    27961 cgcggctacg cccgccaaga ccgtgtcaaa gaacaaatta tgcgcgagct tgccgaactc
    28021 gtccgtaccg gactgaaaga cccgcgcgcc ggcttcatca ctatcaacga agtcgaaatt
    28081 acccgcgatt acagccacgc caccgtgttc tacaccgttt tgaaccaaga tacgcgcgaa
    28141 attacggaag aagtgctgga acacgcgcgc ggacacctcc gcagcgaatt gtccaaacgc
    28201 atcaagctgt tcaaaatccc cgaactgcat ttcaaatacg acgaatcttt ggaacgcggt
    28261 atgagcctgt ccgcccttat cgatcaagtg gcggcggaaa aaccggttga agactgatac
    28321 tgccagaatg aacaatctga ttcccgaaca tttagccgcc tatgcacata gtgataacct
    28381 gcaaattgaa ggcgggcatc gttgcttttc attatcctgc caaggtagag atactttcca
    28441 catccgttac tatggagagc cttttgatgg attgattacc gatactgata aggcgccggt
    28501 aaaaattgtg gcggtagaag ctgtaagcgg cgatgaaatc gtattgtttg atggggcgga
    28561 acatggctat aacgctatgt tctgcgacaa atatagccaa aatcaaaagc aaaacagaac
    28621 gttaactgat ttggatgaat atacttaccg agttccgatt catctttatt acaatataga
    28681 ctacgaagat gaatatgaag atttcgtcaa ttctgaagga caagtcccct taattgatgg
    28741 ccgcatcatt agttttgact cattaaaacg aaatggcttt gatgcaatca gcattgatct
    28801 aattgatgaa aaacactctg ttcgtgaatt attgaatgaa gaattaagct gacacgcagg
    28861 ccgtccgaac cctttcagac ggtctgaaca ttaatattcc ctaattaata tgaccaataa
    28921 acccgccaaa cgcccggtca acggcgttct tcttctcgac aaacccgaag gcctttccag
    28981 caacaccgcc ctgcaaaaag cgcggcgttt gtttcatgcc gaaaaagcag ggcataccgg
    29041 cgtgctcgat cctttggcaa ccggactttt gcccgtctgc ttcggcgaag cggccaagtt
    29101 cgcccaatac ctgctggatg ccgacaaagc ctacaccgcc acgctgaaac tcggcgaagc
    29161 cagcagcacg ggcgatgccg aaggagaaat cattgccgcc gcccgcgccg atatttcctt
    29221 agccgaattt cagacggcct gccaagcact gacaggcaac atccgccaag tgccgccgat
    29281 gttttccgcc ctcaagcacg aaggcaaacc gttgtacgaa tacgcgcgca aaggcattgt
    29341 tatcgaacgc aaaccgcgcg acattaccgt ttactctatc gatattgccg agtttgacgc
    29401 gcccaaagcc gtgataagcg tgcgttgcag caaaggcacc tacatccgca ccctcagcga
    29461 aggcatcgcc aaacacatcg gcacattcgc ccacctgacc gccctacgcc gcaccgaaac
    29521 cgccggcttt accatcgccc aaagccacac gctcgaagct ttggcaaatt taaacgaaac
    29581 agaacgcgac ggtctgctgc tgccctgcga cgtattggtt tcacactttc cccaaaccgt
    29641 tttaaacgat tatgccgtcc atatgctcca atgcgggcaa cggccgcgtt tcgaggaaga
    29701 cctgccttcc gatacgccgg tacgcgttta cacggaaaac ggccgctttg tcggtttggc
    29761 ggaatatcaa aaagaaatat gccgtctgaa agccttgcgc ctgatgaaca cggcggcatc
    29821 cgccgcctga gcggcggtta aaaatacggg ctgtgtttga ataatatgtt gatatttccg
    29881 caaaatcccg atgcattcgg acacccgccc cgcttatcgc aacgttgcga acgcccccgg
    29941 aaacagcaaa gacagcaaag aattgatttt attataattt atttgtaaag ccatttgccg
    30001 ttacacaaga atggcacatt aaaataactg atgaggattt ataacgatga agacaggcat
    30061 tcaaaccgaa ttagcccaag ccctgctacc acacgaaaaa atatgggcga acgaagaaaa
    30121 aaccattttg gccaaaaaca tcctgttgga tttggtggaa aaaaccgacc cgtccattat
    30181 cggtttgtta ttgaataatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt
    30241 tttggtgttt aaattgcagg attttcgttt tttcttggac aaacacagca tcaataattc
    30301 ctacacaaaa tacgccaacc gcattggttt gacggacggc aaccgctttt tgaaagacaa
    30361 ttcggatatt gtgttggatt ttccgtttaa agattgcgta ttgaatggcg gacaaagcac
    30421 cgaagaaggc gaagaaattt attttaaacg caataatagt cagccagcca gccagccagc
    30481 cagccagtca gccagccaat tatacaccaa attaaccaga aaaagacaag aaatcttttt
    30541 taatcaaacg cttgcttttg atgaaattga ccggcttttt gacgcaaaag cattctcaaa
    30601 attctcccgc cacaccgcag acggcaaaca acccgttggc gaaatcaaac gccgttcaga
    30661 cggtacgcca gccgaaaatc ttattatcaa aggcaataat ctgattgccc tgcattcgct
    30721 tgccaagcag tttaaaggca aagtaaagtt gatttatatt gatccaccgt ataatacaga
    30781 aaccgatagc tttgcatata acgataaatt ttcacattcc acttggctaa catttatgaa
    30841 aaatcgcttg gaaattgcga aagagttatt aaaagatgat gggctaatct ttgttcaatg
    30901 cgatgataag gaacaggctt atttaaaagt tttattagat gaaactttta caagagaaaa
    30961 ttttataaac tgcattgctg taaaaatgtc agagccatca ggtaacaaaa tggcacatac
    31021 atcgcataga ctaccaaaga taaaggaata tattcttatc tataagaata aaaatataaa
    31081 attaaaccct attagagagc aaaaatcaga atgggacaat gagtacaata ttttttagaa
    31141 aattttacac aagaagataa aaaatttatt gatttaatag taaatagcca aacagaaaat
    31201 aaagaaataa atggtaatac tttaaaagaa attgatattc ttttaaagaa aatatctcct
    31261 atttcagtta atcaaaaatt agcccaatta aatattaaag ataatgaagt tataaaatgg
    31321 aaactagata atgcgtataa aattgtaaga acagcagcta gtagtagcgt aaaaaaatta
    31381 gcagatgaaa aaaaagaaat ttgccaacaa caattttttt cagttatttc taaacgagat
    31441 aaattattgt atatcgtcaa gtcagattac tcaaaagatg caaaagcacc acgagtgcag
    31501 gttttgtttg cagaagatta tttgagtatt agtttatgcg atttatggac aaatataaat
    31561 accacaggac tagaagcaga aggaaatgta gagttaaaaa atggtaaaaa gccagaaagt
    31621 ttaattgaaa ccatcattaa actagcaacc aacgaaaacg acatcgttct agactaccac
    31681 ttgggcagtg gcacaaccgc cgccgtcgcc cataaaatga accgccaata catcggcatt
    31741 gaacaaatgg attatattga aacgcttgcc gttgaacgca tgaaaaaagt aattgacggc
    31801 gagcaaggcg gtatctcaaa agctgtgaat tggcaaggtg gtggcgagtt tgtttatgcc
    31861 gaactttccc catttaacga aaccgcaaaa caacaaattt tggcgtgcga aaattcagac
    31921 ggcatcaaaa cgctgtttga aggtttatgc gaacgccatt tcttgaaata caacgtcagc
    31981 gtaaatgaat ttagccaaat tattgaagag cctgaatttc aatccttggc attagacgaa
    32041 caaaaacaaa tgatgcttga aatgttggat ttaaatcaaa tgtatatttc attatccgaa
    32101 atggacgatg aacaatttgc aggctgcctg aatgatgatg ataaagcgtt aagccgtgcg
    32161 ttctatcaat cagtaaaaca tcaagcggag aaaaaagatg gcgaataata aaacgttgtt
    32221 tgattgggtg gaagaccgca aatcaatgct ggaagaaatg gaacagacgg attttttcgc
    32281 gctgcctgaa tttgtttcca ataatttaaa atatccgttt tttgaatggc aaaaatcggc
    32341 tttagaaaat tttgtgattt ttgaccgcac ttcaaaatta aaggatttcc ctgacatcaa
    32401 aaaccgccct acccatttgc tgttcaatat ggcaacaggt gcaggcaaaa cgatgatgat
    32461 ggcggcattg attctgtatt attttgagaa agggtatcgg cattttttat ttttcgtcaa
    32521 taaaaacaat atagtatata aaacggaaaa taattttatc gatccgacgc atccaaaatt
    32581 tttatttacc gagaaaattt tgcaaggtga tacggtaatt cccattcgca aagtggagac
    32641 atttagccaa tattcagacg gcattgaaat taaatttacc agcattcaaa aactatataa
    32701 cgatattcac accgagcgag aaaatcaaac cacattggcg gatttgcacg aattaaatct
    32761 tgtgatgctg ggcgatgaag cacaccattt aaacgcgcaa accaaaggca aaaaacaagg
    32821 cgaattagat ttagaaaagg aaatgaacga gcgtaccagc gatgccgaaa ttgaacgcaa
    32881 aggctgggag catatggttt tggagttgtt actcaataaa aatggcaatc ccggccaaaa
    32941 tgtgctgttg gaatttaccg ccacactgcc tgaaaatgct gaagtgcaac aaaaatatgc
    33001 ggataaaatc atcacaaaat ttgggttaaa agaatttttg caaaaaggtt ataccaaaga
    33061 aatcaatttg gtatccggta cgctgaataa gaaagagcgg gtgttgcacg ctttattgtt
    33121 tgcctggtac cgccatcaaa tcgcgttgaa atacggcatt gccaatttca agcctgtgat
    33181 gttgtttaga agtaagacga ttgatgaatc aaaagcggat tattcggcgt ttttaaattg
    33241 ggtagaaaat gtgcaagcgg atgatttttc gtttttaact acattttcaa caagcttaag
    33301 cgatagcaat aacgccaacg aacaaggcaa aacccgtacc gaacaagccc taaaatttat
    33361 gcaggaaaat aaatttgagt ttgtgcattt ggcaaattgg gtgaaacaaa attatcaaaa
    33421 acataatgtc attattacca actccgaaac caacaaaacc aaaactgaaa aaaccgacag
    33481 cgaaaccgaa aaattgctaa acaatttgga agcggctgat aatccgatct gtgccatttt
    33541 tacggtggat agattaaccg aaggttggga cgttttaaat ttgtttgata ttgtgcgttt
    33601 gtatgaaggg caaaacggtg gcggttcgaa taaaaaatcg ggcaaaacgg ctgccgccac
    33661 cgtatcggaa aagcagttga ttggtcgcgg cgtgcgttat tttccatttg cgtttgaagg
    33721 taaacagccg aataaacgca aatttgacaa cgatatgcaa catgaattgc gtatcttgga
    33781 agaattgttt tattacacgc acgatgagca atcccgctat attacagaac tgaaaaacga
    33841 gttacgaaaa gacggttatt tgcctgaaaa agacgatgat aaggtattga caacatttaa
    33901 gctcaaatct gaatttgcag ataataagga ttttagagag ttgttaattt gggcgaataa
    33961 aaaaatcccc aacccaaacg ccaaaagcaa taatgcagac agcctgcaag ccaatccgca
    34021 aacgctttca ttccaagttc acggtaatca actgttgcag gaaacgcaat ttacagccga
    34081 tgaaaatgat gaaacagccc gacaaatcgg cacacaaaat aattttactc aaaccataaa
    34141 aatgagtgaa atggagcggc atattttcaa taaagcatta catatcaaag ggaaaaatag
    34201 tcaatcttta ttccattttg accgcctgca aagcaaactc gacattcaaa atcgcaatga
    34261 attgcaaaat aaattgttga aagactggca gattgaattt ttgggattag agcaagataa
    34321 acaggttcgc ccggatgata aacttgcagg ctgcctgaaa atcttggaaa tggttgaaaa
    34381 acatctgaat gaaagtgata taccgtttat cggtacaaaa gaatttacac ctaaaaaatt
    34441 gtgggaaatt ttcggcacgc caaaacaaaa atgggtcaaa aaagatgata taaaaactgc
    34501 tattgccacg caaaatgatt ggtatgtgat ggataatttt gccggaacgg gtttggaaga
    34561 agcgttaatt caatttattt cagcgcgctt gggcgatttg aaatctcaat atgacgttca
    34621 tttaatccgc aatgaagaag tgtttaaatt gaataacttt gccgatggcg aaggatttat
    34681 gccggacttt gttttattgc tgaaagataa acaaaaatct tcttccgaca gcgtggacga
    34741 ctttttgcat taccaaattt tcattgaacc gaaaggcggg catttagtgg aaaatgattc
    34801 gtggaaagaa gcttttttaa aatcaatcac agtggaatat gggagggata agatcctgca
    34861 aaaaaataca ccgcattatc gtttgatcgg tttgccgttt tttactgatc atcagaaaaa
    34921 tggacaattt acagagttat tccctttagg tgcggcatcg cttgaaaaat agagtagtgc
    34981 attgcaggca gccccgttcg gcaaaacttc ctttacaaaa gggcgttttg tcagatattt
    35041 aatcaacaca ttattaaaat acaaccaaat tttaatgccg tccgaaccat gtattcagac
    35101 ggcatcgtat ttttcagtat ctaaaccgtt tccctgcccc aatccttact cctcaaaatc
    35161 gaggcatcga catcttgaat atcgcggtgt cccgtaaacg ccatcgacac atccatttcc
    35221 ttgtacaaaa tctccagcgc gcgcgttacg ccttcttcgc catacgcacc caaaccatac
    35281 aggaacgccc gacctatcat cgtgcctttc gcacccaaag cccacgcctt caaaatatcc
    35341 tggccgctgc ggataccgct gtccatccaa acttcgatgt cgctgccgac tgcggaaaca
    35401 atatcgggca aggccttgat ggcggacaca gtatcgtcga gctggcggcc gccgtggttg
    35461 gaaacgacca atgcatccgc accgcttttc gccgcctttt ccgcgtcttc gggttccata
    35521 atgcctttga taatcagctt gccgccccac aaatctttaa tccgcgccac gtcgtcccag
    35581 cttaagcgcg ggtcgaactg ttccgccgtc cacgaagaaa gtgaagacaa atcgccgacg
    35641 tttttggcgt gtccgacgat attgcggaac gtgcggcgtt ccgtgttcag cattttcatg
    35701 caccattcgg gcttggtcgc cagattgatt aaattggcga tggtcggttt cggcggcgcg
    35761 gacaggccgt ttttgatgtc tttgtggcgt tgacccaaaa cctgcaaatc ggcagtcaat
    35821 accaatgccg aacatttggc atctttcgca cgcttaatca ggttttccat aaattcgcgg
    35881 tcgcgcatca cataaagctg aaaccaaaac ggcgcgctgg tgttctcggc aacgtcttca
    35941 atcgagcaga tggacatggt ggacaacgta aacggaatac cgaacttctc cgccgcacgc
    36001 gccgccaaaa tttcgccgtc tgcgtgtgcc atgccggtaa aacccgtcgg cgcaatcgcc
    36061 accggcattt tcacatcccc cccgatcatt ttggtttcga ggctgcgtcc ctccatattg
    36121 accaatacct tttgtcggaa gcggatatct ttgaaatccg aagtattctc cctataagtc
    36181 gtttccgtcc acgaacccga atcgatgtaa tcgtaaaaca tacgcggcat tttgcgcttg
    36241 gcaacgcggc gcaagtcttc gatgcaggtc attttgctca aatcacgttt catcttgttt
    36301 ttccctgttt aacagcctga tggaatatgc cgtctgaaac tgcttgagcc ttcaaacggc
    36361 atttcgggca atacccaaat aaatcactaa gatttagata atctatatca atattaaaaa
    36421 taaatcaaat cgttttgaaa ccgcttgccg gcaaaacgcc ccaaccgccg ctcctcaaaa
    36481 aacataacta actgattcaa aaaaccaaac aaataaagca aaatcagact ataccgccat
    36541 ttaaacaaac aatcggcaac agctccccca tacttgactg aaacactcag atattggaca
    36601 attccgccca ctataaaaaa gccgacacgg gcaaccacca ccatgagact gaccaccaaa
    36661 gggcgtttcg ccgttaccgc tatgctggat ttggcgatga acgcgcaaac cggcgccgtc
    36721 aaactcagtg ccatcagcga acgccaaaat atatccctct cctatctcga gcaattgttc
    36781 ggcaaactcc gccgcgccgg actggttgaa agcctgcgcg ggcccggcgg cggctacatc
    36841 ctcgccgccc ctcccgcaca aatcaacatc gcccaaatca tttccgccgc cgaagaccgt
    36901 ctggacgcga cccaatgcgg cagcaaagcc aactgccacc acggcgcgcc ctgcctgacg
    36961 cacgaccttt gggagaattt aaacaaaacc atcaacgact atctcggcag cgttaccctg
    37021 caaagcatca tcgaacagaa aaacaacggc gacggcagcc gcgtcgtcca atttacacac
    37081 atccattaaa taacacccga aaaagaaaga gcaaaccatg accgtcaaaa cccccgttta
    37141 cctcgactac gccgccacca cacccgttga caaacgcgtt gccgaaaaaa tgattcccta
    37201 tctgactgaa accttcggca accccgcctc caacagccac gcattcggct ggacggcaga
    37261 agaagccgtc gaaaaagccc gcgccgacat cgccgccctg attaacgccg accccaaaga
    37321 aatcgtcttc accagcggcg cgaccgagtc cgacaacctc gccatcaaag gtgcggcaaa
    37381 cttctacaaa accaaaggca aacacctcat caccgtcaaa accgaacaca aagccgtgct
    37441 cgacacgatg cgcgaactcg aacgccaagg ctttgaagtt acctacctcg gcgtgcagga
    37501 aaacggtttg attgatttgg aagaactcaa agccgccatc cgcgacgaca ccatcctgat
    37561 ttccataatg tgggtgaaca acgaaatcgg cgtggtgcaa aacattcccg ccatcggcga
    37621 aatctgccgc gaacgcaaaa tcgccttcca cgtcgatgcc gcccaagcct gcggcaaagt
    37681 gcctgtcgat gtcgaagccg ccaaaatcga cttgctctcg atgtccgcgc acaaagtgta
    37741 cggccccaaa ggcatcggcg cgctgtacgt ccgccgcaaa ccgcgcgtcc gcctcgaagc
    37801 ccaaatgcac ggtggcggtc acgagcgcgg tttccgttcc ggcacattgc cgacccatca
    37861 aatcgtcggc atgggcgagg ccttccgcat cgccaaagaa gaattggcac aagacacagc
    37921 gcactacctg aaactgcgcg acatcttcct caaaggcatc gaaggcatcg aagaagtcta
    37981 tatcaacggc gacctcgaac accgcgcccc gaacaacctg aacgtcagct tcaacttcgt
    38041 cgaaggcgaa agcctgatta tggcggtgaa agaactcgcc gtatccagcg gatccgcctg
    38101 cacctccgcc tcgctcgaac ccagctacgt cctgcgcgcg cttggccgca acgacgaact
    38161 ggcgcactca tccctgcgca tcaccttcgg ccgcatgacc accgaagaag aagtgcaatt
    38221 cgcggcagaa ctgatcaaat ccaaaatcgg caaactgcgc gaactgtcgc cgctgtggga
    38281 aatgttcaaa gacggtattg atctgaactc gattgaatgg gcggcgcatt aagattgatg
    38341 tcaaacaaag gctgtctgag aaagaatacc atgaaagtta aaattattaa tttgccaaat
    38401 ggttataaac gtattattta tggaaaatat tttgaacaat ttgacttaga ttatgaacaa
    38461 gatttagacg tattaaaaaa agatattgaa tttgctttat ctgttattga atacaatcga
    38521 tctatattca aaaaattctc ttccttattt gagaataaaa tcatttttgt ttatcaagga
    38581 ggacatcatc ttgatattat tgatcgtgat aaaggaagtt tgaaatagtt aactattgaa
    38641 gatcattgga caaaagatgt tgatgatatc actggttcat ggaaaatgga gaatttagaa
    38701 attagccgac aaactcaata tgcaatgcaa cttctaaaga ttggtaaaac aaatattgag
    38761 aaagaaataa gaaaaattaa acaaaggttc taaaaaaatg agtaaatacg gtggtgaaga
    38821 cgtaatctat atgaatgatg gtgtatttag aaataaatta gccattcaag atccacttaa
    38881 atttaaaaaa ggtcgaaaga gacatttctg ctatttccgc ccaaaatctg cttttgcaac
    38941 ccattacagg caatttcgat ttggcgcatt tacagaccat tcatcgcgaa ctgtttggca
    39001 atgtttatga ttgggcagga aaaatccgga gggttgatat ttctaaaggt aatacccgat
    39061 ttgccaattt tgcttttatc gaaaacgaaa gccgtaaact tttggagaag ctgaaaaacg
    39121 aaaactattt gcgggtgttg gataaagata aattcgccga gcgtgcagcc tattatttgg
    39181 acgagttgaa cgtcctacat ccttttcgtg aaggaaacgg cagaacattg cgcctgttta
    39241 tgacacaatt ggcaataaaa aacggttttc agatacattg gcaaaatatc tctgccgaac
    39301 aaatgattca agcctgtatt caggcatacc atgccgatag cagcttattg gcacgcctaa
    39361 ttattagaca atttagaaca atcgtctttt tcagacgact aaaacatcaa caaaccacga
    39421 tattttaagg aaaccacatc atggcataca gcgataaagt aatcgaccac tacgaaaatc
    39481 cccgcaacgt cggcactttc gacaaaaacg acgagtccgt cggcaccggc atggtcggcg
    39541 cgcccgcctg cggcgacgtg atgcgcctgc aaatcaaagt gaacgatgaa ggcatcatcg
    39601 aagatgcgaa attcaaaact tacggctgcg gttccgccat cgcttcgtcc agcctgatta
    39661 ccgagtgggt caaaggcaaa agtctggatg acgcgctggc aatcaaaaac agcgaaatcg
    39721 ccgaagaact ggaattgccg ccggtaaaaa tccactgctc catcttggct gaagatgcgg
    39781 taaaagcggc cgttgccgac taccgcaaac gtcaggaaaa cagataaagc ccttcagacg
    39841 gcatcgtccc acaatgccgt ccgaaccgcc cgccgcttcg gatccgtccg gggcggtaca
    39901 acaaggaaga aatatgatta cccttaccga gaatgccgca aaacacatca atgactatct
    39961 cgccaaacgc ggcaaaggct tgggcgtacg cttgggtgta aaaaccagcg gctgctcggg
    40021 gatggcgtac aaccttgaat ttgtcgatga agccaacggc gacgacctga ttttcgaagg
    40081 acacggcgcg cgcatttata tcgacccgaa aagcttggtt tatctggacg gcacacaagt
    40141 cgattacacc aaagaagatt tgcaggaagg tttcaaattt gaaaacccca atgtcaaaga
    40201 ctcctgcggc tgcggcgaga gcttccacgt ttaaggcata aaaacggcgg gacagtatca
    40261 aaaccgtccc gccgtttttt cgcttcctgc ctgttgtagc tgcctttgcc tttccttttc
    40321 cgttccacct tgtgccggaa caaatcggat tttactaagg cttttaaagc attgtcgcgt
    40381 attttgcctt tattgtgctg cgctttgccg cccatattca gtcctttcgt ttaagaagcc
    40441 gcagattata aggcaaaaac agttttctgc caaaatctta catttatcat cctactatgt
    40501 cccaatattt caccctcttc cggattgaac ccgctttcga tatcggcacc gaaaacttgg
    40561 aacaaaccta ccgcgccttg gccgcccgtt tccatcccga caaattcgct tccgcttccg
    40621 ctttcgagca gaaacaggcg gttatgatgt cttccaccat caacgatgcc taccgcacct
    40681 tgaaaaatcc catcgaccgc gccgcgtacc tgctgaaaac atcgggtatc gatgccgacg
    40741 cgccggaaca tacctctttc gcccccgact tcctcatgca gcaaatggaa tggcgcgaaa
    40801 cgctgatgga ggcacgggca ggcaacaacc ttgaatcctt gaaaaatctc gataacgaaa
    40861 tccgcgccga acaagaaaaa ctgttctgcg gtctgaaaca gtcattcgcg cgccaagact
    40921 gcgacaccgc cgcacaacaa gtccgccaag gcaggtttct cgacaaactc cgccacgaaa
    40981 tttcctccgc attataatcc gcaccgtgtt tcagacggca taaccgccgc accgtttcat
    41041 gtcgaaatat gctaaaataa gcaacaattt tttgccatac gaaacattga aaccatgacc
    41101 gacgcaacca tccgccacga ccacaaattc gccctcgaaa ccctgcccgt cagccttgaa
    41161 gacgaaatgc gcaaaagcta tctcgactac gccatgagcg tcattgtcgg gcgcgcgctg
    41221 ccggacgttc gcgacggcct aaagccggtg caccggcgcg tactgtacgc gatgcacgag
    41281 ctgaaaaata actggaatgc cgcctacaaa aaatcggcgc gcatcgtcgg cgacgtcatc
    41341 ggtaaatacc acccccacgg cgattccgca gtttacgaca ccatcgtccg tatggcgcaa
    41401 aatttcgcta tgcgttatgt gctgatagac ggacagggca acttcggatc ggtggacggg
    41461 cttgccgccg cagccatgcg ctataccgaa atccgcatgg cgaaaatctc acatgaaatg
    41521 ctggcagaca ttgaggaaga aaccgttaat ttcggcccga actacgacgg tagcgaacac
    41581 gagccgcttg tactgccgac ccgtttcccc acactgctcg tcaacggctc gtccggtatc
    41641 gccgtcggta tggcgaccaa catcccgccg cacaacctca ccgacaccat caacgcctgt
    41701 ctgcgtcttt tggacgaacc caaaaccgaa atcgacgaac tgatcgacat tatccaagcc
    41761 cccgacttcc cgaccggggc aaccatctac ggcttgggcg gcgtgcgcga aggctataaa
    41821 acaggccgcg gccgcgttgt tatgcgcggt aagacccata tcgaacccat aggcaaaaac
    41881 ggcgaacgcg aagccatcgt tatcgacgaa atcccctatc aggtcaacaa agccaagttg
    41941 gtcgagaaaa tcggcgattt ggttcgggaa aaaacactgg aaggcatttc cgagctccgc
    42001 gacgaatccg acaaatccgg tatgcgcgtc gttatcgagc tgaaacgcaa cgaaaatgcc
    42061 gaagtcgtct taaaccaact ctacaaactg actccgctgc aagacagttt cggcatcaat
    42121 atggtggttt tggtcgacgg acaaccgcgc ctgttaaacc tgaaacagat tctctccgaa
    42181 ttcctgcgcc accgccgcga agtcgttacc cgacgtacgc ttttccggct gaagaaggca
    42241 cgccatgaag ggcatatcgc cgaaggcaaa gccgtcgcac tgtccaatat cgatgaaatc
    42301 atcaagctca tcaaagaatc gcccaacgcg gccgaggcca aagaaaaact gcttgcgcgc
    42361 ccttggcgca gcagcctcgt tgaagaaatg ctgacgcgtt ccggtctgga tttggaaatg
    42421 atgcgtccgg aaggattggc tgcaaacatt ggtctgaaaa aacaaggtta ttacctgagc
    42481 gagattcagg cagatgctat tttacgcatg agcctgcgaa acctgaccgg cctcgatcag
    42541 aaagaaatta tcgaaagcta caaaaacctg atgggtaaaa tcatcgactt tgtggatatc
    42601 ctctccaaac ccgaacgcat tacccaaatc atccgtgacg aactggaaga aatcaaaacc
    42661 aactatggcg acgaacgccg cagcgaaatc aacccgttcg gcggcgacat tgccgatgaa
    42721 gacctgattc cgcaacgcga aatggtcgtg accctgaccc acggcggcta tataaaaacc
    42781 cagccgacca ccgactatca ggctcagcgt cgcggcgggc gcggcaaaca ggcggctgcc
    42841 accaaagacg aagactttat cgaaaccctg tttgttgcca acacgcatga ctatttgatg
    42901 tgttttacca acctcggcaa gtgccactgg attaaggttt acaaactgcc cgaaggcgga
    42961 cgcaacagcc gcggccgtcc gattaacaac gtcatccagc tggaagaagg cgaaaaagtc
    43021 agcgcgattc tggcagtacg cgagtttccc gaagaccaat acgtcttctt cgccaccgcg
    43081 cagggaatgg tgaaaaaagt ccaactttcc gcctttaaaa acgtccgcgc ccaaggcatt
    43141 aaagccatcg cactcaaaga aggcgactac ctcgtcggcg ctgcgcaaac aggcggtgcg
    43201 gacgacatta tgttgttctc caacttgggc aaagccatcc gcttcaacga atactgggaa
    43261 aaatccggca acgacgaagc ggaagatgcc gacatcgaaa ccgagatttc agacgacctc
    43321 gaagacgaaa ccgccgacaa cgaaaacacc ctgccaagcg gcaaaaacgg cgtgcgtccg
    43381 tccggtcgcg gcagcggcgg tttgcgcggt atgcgcctgc ctgccgacgg caaaatcgtc
    43441 agcctgatta ccttcgcccc tgaaaccgaa gaaagcggtt tgcaagtttt aaccgccacc
    43501 gccaacggat acggaaaacg caccccgatt gccgattaca gccgcaaaaa caaaggcggg
    43561 caaggcagta ttgccattaa caccggcgag cgcaacggcg atttggtcgc cgcaaccttg
    43621 gtcggcgaaa ccgacgattt gatgctgatt accagcggcg gcgtgcttat ccgtaccaaa
    43681 gtcgaacaaa tccgcgaaac cggccgcgcc gcagcaggcg tgaaactgat taacttggac
    43741 gaaggcgaaa ccttggtatc gctggaacgt gttgccgaag acgaatccga actctccggc
    43801 gcttctgtaa tttccaatgt aaccgaaccg gaagccgaga actgaaaatc atctcccgat
    43861 gccgtctgaa gattcagacg gcatttattt ttccctcatc cgtcatccag cttctcacaa
    43921 tatagcggat taacaaaaat caggataagg cgacgaagcc gcagacagta caaatatagt
    43981 ggattaaatt taaactagta cagcgttgcc tcgccttgcc gtactatagt caat
//
LOCUS       DAXFHF010000008        41252 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXFHF010000008 DAXFHF010000000
VERSION     DAXFHF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41252
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.008"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..276
                     /locus_tag="ACFIRM_000369"
     CDS             <1..276
                     /locus_tag="ACFIRM_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3028073.1"
                     /translation="LSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFP
                     KLINNLKKAGKPKMVIIVAIMRKLAKPAYYIVKTGQPYDAERHRLNQ"
     gene            566..1105
                     /locus_tag="ACFIRM_000370"
     CDS             566..1105
                     /locus_tag="ACFIRM_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC3028074.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLTAAAATGAQGAGEA"
     gene            1105..1263
                     /locus_tag="ACFIRM_000371"
     CDS             1105..1263
                     /locus_tag="ACFIRM_000371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028075.1"
                     /translation="MMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAESEIKNYKAM
                     LKTYERPD"
     gene            1247..1588
                     /locus_tag="ACFIRM_000372"
     CDS             1247..1588
                     /locus_tag="ACFIRM_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028076.1"
                     /translation="MNGLIKNPKLIAVSVCAVFVAGAWQYDRAAQYRRGYGAAVSEVS
                     ERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADGV
                     RELNAAVDDGG"
     gene            2078..2458
                     /locus_tag="ACFIRM_000373"
     CDS             2078..2458
                     /locus_tag="ACFIRM_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC3028077.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(2659..3501)
                     /locus_tag="ACFIRM_000374"
     CDS             complement(2659..3501)
                     /locus_tag="ACFIRM_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3028078.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSAAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(4000..4176)
                     /locus_tag="ACFIRM_000375"
     CDS             complement(4000..4176)
                     /locus_tag="ACFIRM_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028079.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(4504..4995)
                     /gene="dtd"
                     /locus_tag="ACFIRM_000376"
     CDS             complement(4504..4995)
                     /gene="dtd"
                     /locus_tag="ACFIRM_000376"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC3028080.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(5069..5827)
                     /locus_tag="ACFIRM_000377"
     CDS             complement(5069..5827)
                     /locus_tag="ACFIRM_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3028081.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(5897..6763)
                     /locus_tag="ACFIRM_000378"
     CDS             complement(5897..6763)
                     /locus_tag="ACFIRM_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3028082.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKLSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(6812..7090)
                     /locus_tag="ACFIRM_000379"
     CDS             complement(6812..7090)
                     /locus_tag="ACFIRM_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3028083.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(7090..7380)
                     /locus_tag="ACFIRM_000380"
     CDS             complement(7090..7380)
                     /locus_tag="ACFIRM_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC3028084.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(7383..7913)
                     /locus_tag="ACFIRM_000381"
     CDS             complement(7383..7913)
                     /locus_tag="ACFIRM_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC3028085.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTVLMLFF
                     FIIQGIYLSTYLKKED"
     gene            8221..10537
                     /gene="tspA"
                     /locus_tag="ACFIRM_000382"
                     /pseudo
     CDS             8221..10537
                     /gene="tspA"
                     /locus_tag="ACFIRM_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(10710..11126)
                     /gene="gloA"
                     /locus_tag="ACFIRM_000383"
     CDS             complement(10710..11126)
                     /gene="gloA"
                     /locus_tag="ACFIRM_000383"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC3028086.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(11151..12320)
                     /gene="lapB"
                     /locus_tag="ACFIRM_000384"
     CDS             complement(11151..12320)
                     /gene="lapB"
                     /locus_tag="ACFIRM_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC3028087.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(12331..12645)
                     /locus_tag="ACFIRM_000385"
     CDS             complement(12331..12645)
                     /locus_tag="ACFIRM_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC3028088.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            12955..13953
                     /gene="ilvE"
                     /locus_tag="ACFIRM_000386"
     CDS             12955..13953
                     /gene="ilvE"
                     /locus_tag="ACFIRM_000386"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC3028089.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(14198..14983)
                     /gene="fabI"
                     /locus_tag="ACFIRM_000387"
     CDS             complement(14198..14983)
                     /gene="fabI"
                     /locus_tag="ACFIRM_000387"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC3028090.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(15138..15959)
                     /gene="dapD"
                     /locus_tag="ACFIRM_000388"
     CDS             complement(15138..15959)
                     /gene="dapD"
                     /locus_tag="ACFIRM_000388"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC3028091.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(16169..17812)
                     /gene="pgi"
                     /locus_tag="ACFIRM_000389"
     CDS             complement(16169..17812)
                     /gene="pgi"
                     /locus_tag="ACFIRM_000389"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3028092.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            18322..19554
                     /gene="pilG"
                     /locus_tag="ACFIRM_000390"
     CDS             18322..19554
                     /gene="pilG"
                     /locus_tag="ACFIRM_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC3028093.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            19628..20488
                     /locus_tag="ACFIRM_000391"
     CDS             19628..20488
                     /locus_tag="ACFIRM_000391"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC3028094.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            20485..21117
                     /gene="coaE"
                     /locus_tag="ACFIRM_000392"
     CDS             20485..21117
                     /gene="coaE"
                     /locus_tag="ACFIRM_000392"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC3028095.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            21110..21319
                     /locus_tag="ACFIRM_000393"
     CDS             21110..21319
                     /locus_tag="ACFIRM_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC3028096.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(21405..23081)
                     /gene="pilB"
                     /locus_tag="ACFIRM_000394"
     CDS             complement(21405..23081)
                     /gene="pilB"
                     /locus_tag="ACFIRM_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC3028097.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(23327..23398)
                     /locus_tag="ACFIRM_000395"
     tRNA            complement(23327..23398)
                     /locus_tag="ACFIRM_000395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23363..23365),aa:Arg,seq:cct)
     gene            complement(23419..23865)
                     /locus_tag="ACFIRM_000396"
     CDS             complement(23419..23865)
                     /locus_tag="ACFIRM_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC3028098.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(23869..24843)
                     /locus_tag="ACFIRM_000397"
     CDS             complement(23869..24843)
                     /locus_tag="ACFIRM_000397"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3028099.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            25067..25375
                     /gene="rplU"
                     /locus_tag="ACFIRM_000398"
     CDS             25067..25375
                     /gene="rplU"
                     /locus_tag="ACFIRM_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC3028100.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            25400..25672
                     /gene="rpmA"
                     /locus_tag="ACFIRM_000399"
     CDS             25400..25672
                     /gene="rpmA"
                     /locus_tag="ACFIRM_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC3028101.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            25907..27091
                     /gene="ubiM"
                     /locus_tag="ACFIRM_000400"
     CDS             25907..27091
                     /gene="ubiM"
                     /locus_tag="ACFIRM_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC3028102.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(27280..27435)
                     /gene="rpmG"
                     /locus_tag="ACFIRM_000401"
     CDS             complement(27280..27435)
                     /gene="rpmG"
                     /locus_tag="ACFIRM_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC3028103.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(27467..27700)
                     /gene="rpmB"
                     /locus_tag="ACFIRM_000402"
     CDS             complement(27467..27700)
                     /gene="rpmB"
                     /locus_tag="ACFIRM_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC3028104.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(27950..29473)
                     /gene="farB"
                     /locus_tag="ACFIRM_000403"
     CDS             complement(27950..29473)
                     /gene="farB"
                     /locus_tag="ACFIRM_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC3028105.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(29497..30651)
                     /gene="farA"
                     /locus_tag="ACFIRM_000404"
     CDS             complement(29497..30651)
                     /gene="farA"
                     /locus_tag="ACFIRM_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC3028106.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      30892..30936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30940..31413
                     /gene="queF"
                     /locus_tag="ACFIRM_000405"
     CDS             30940..31413
                     /gene="queF"
                     /locus_tag="ACFIRM_000405"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC3028107.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            31488..32174
                     /locus_tag="ACFIRM_000406"
     CDS             31488..32174
                     /locus_tag="ACFIRM_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC3028108.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(32524..33816)
                     /locus_tag="ACFIRM_000407"
     CDS             complement(32524..33816)
                     /locus_tag="ACFIRM_000407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC3028109.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGGTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            33893..34861
                     /locus_tag="ACFIRM_000408"
     CDS             33893..34861
                     /locus_tag="ACFIRM_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028110.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(35062..36531)
                     /locus_tag="ACFIRM_000409"
     CDS             complement(35062..36531)
                     /locus_tag="ACFIRM_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3028111.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGLSVTREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(36773..37030)
                     /locus_tag="ACFIRM_000410"
     CDS             complement(36773..37030)
                     /locus_tag="ACFIRM_000410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3028112.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            36956..37117
                     /locus_tag="ACFIRM_000411"
     CDS             36956..37117
                     /locus_tag="ACFIRM_000411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028113.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            complement(37214..37627)
                     /locus_tag="ACFIRM_000412"
     CDS             complement(37214..37627)
                     /locus_tag="ACFIRM_000412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028114.1"
                     /translation="MSFDCFGAFSYQPGSDTRPTNVGDTADFSFSDKPANGVFHYFSS
                     GKTDQKSSEALYDEINITDNYRTDLKQAVKKQLQDLYKTRPEAWEENKKRVREEIIKQ
                     FGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            complement(38154..38399)
                     /locus_tag="ACFIRM_000413"
     CDS             complement(38154..38399)
                     /locus_tag="ACFIRM_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028115.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            38511..38939
                     /locus_tag="ACFIRM_000414"
     CDS             38511..38939
                     /locus_tag="ACFIRM_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC3028116.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            38951..39427
                     /locus_tag="ACFIRM_000415"
     CDS             38951..39427
                     /locus_tag="ACFIRM_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC3028117.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(39457..39945)
                     /locus_tag="ACFIRM_000416"
     CDS             complement(39457..39945)
                     /locus_tag="ACFIRM_000416"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC3028118.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKVYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEVALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(40013..41068)
                     /gene="aroG"
                     /locus_tag="ACFIRM_000417"
     CDS             complement(40013..41068)
                     /gene="aroG"
                     /locus_tag="ACFIRM_000417"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC3028119.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
BASE COUNT         9834 a        11556 c        10321 g         9541 t
ORIGIN      
        1 ttaagtcccg ccatcataca atcagggaca agcgtaagag gtcggggcag attgagccga
       61 tacggaaaca gacgattaaa aagtacgctg tatatgcccg ccctttgtgc ttaccgtttt
      121 aacgcatttc cgaaattaat aaataatctg aaaaaagcgg gtaagccaaa gatggtaatt
      181 atcgttgcca tcatgcgcaa actggcgaag cccgcctatt acattgttaa aaccggccag
      241 ccttacgatg cggaaagaca ccgattgaat caataaaatt caacaaaatt aaacggttgc
      301 gcgaatatat ttgtgtaacc gtgcatttgc atatcgtaaa taaacgtaaa taaaaataac
      361 aatataaatc agcatgttgc aactttgttt ttattttgtg ttgacgggca acatatcatc
      421 tgcgcggaaa gttaccgtat cggcaaacgc gactgaaaaa gacagcgtgt tcggcgatgc
      481 ggtgcacatt gtgattgcga cttaaaacat agaacgagtt gccaacccaa agtttacatc
      541 tcgtctttta ccgaaaggat taaaaatgac agaattaccg tggatagccg aagcgagaag
      601 gcacatcggt ttgaaagaaa ttcccggcgc gaaacacaat ccgacgattg tgcaatggct
      661 caaagagacg ggcggcttcc ccggcgcggc aaagtcttgg tactttgaag acgaaacgcc
      721 gtggtgcggc ctgtttgtcg gacactgcct gggcaaagcg ggacgcgcgg tcatcaggga
      781 ctggtatcgc gccaaagcct ggtcaatgtc gggtttgacg aaactcgaag cccccgcata
      841 cggctgcatc gcggtcaaac cgcgccgggg cggcggacac gtgttcttcg ttgtcggcaa
      901 agacgcggaa ggcagaatct tgggcttggg cggcaatcag ggcaatatgg tatccatcat
      961 cccgtttgac cctgcggaca ttgacggcta tttctggccg tccaagctga ttggcggcaa
     1021 acctgtgcct tcgtcccccg ccgaagggcg ttaccggttg actgccgccg ccgccaccgg
     1081 cgcgcaaggc gcgggagagg cgtaatgatg aatcctaccg agtttctgaa agcgcgtatc
     1141 gccgaatggg aagccaaaag caaagaggcg ggcggaaacg ccgattttaa agcttttgag
     1201 tttgccgaaa gcgaaatcaa aaactacaaa gcgatgttga aaacttatga acggcctgat
     1261 taaaaacccg aagctgattg cggtgtcggt ttgcgccgtg ttcgttgccg gcgcgtggca
     1321 atacgaccgc gccgcgcaat accgtcgcgg atacggtgcg gcggtgtcgg aagtttcgga
     1381 acgcctcaaa gccgccgcgg tcgaacacgc cgaacacgcc cgcaaatcgt ccgccgcgta
     1441 tcaggcgcaa aaggcggcgc gtgaggaaaa agaaagggtg cgctatgtgc aaacgcttaa
     1501 aatcattgaa aaacctgtgt accgcaatgc ctgttttgat gctgacggcg tgcgcgaact
     1561 caacgccgcc gttgacgacg gcggttaagc cgcccgccga tttggtgcgg ccctgcccga
     1621 aactgccgca ccttgaaggg aacacgggcg cggacgtgct gccgtgggcc ctgaaggcgg
     1681 ccggtatgta taacgactgc agggcgcggc acggcgcgct ggtacgggcg ttgggcgcgg
     1741 attgagttgc caaccgaaag tttgcaaccg aaccgtcggt tccgggttgg cggccgcatc
     1801 gggggaagtg tcggcattcc ccccgatttt ttacatatcg ggcggacgcg gcaaattttt
     1861 gccgttttgt ttgcaagaag ggggcgttat acaaaattat taagcgcacc aataatgggc
     1921 gggaatgaaa atgccgtacc ctatgggcag gcttcgcact ctgaaaggtt caggcggcat
     1981 ttttattgga aggctttttt ttgcaaccgc cttacacaaa gcggtttttt gtgtaagaac
     2041 tgctataata gccgcctgtc atcgtcaggg gcggctaatg cctttaaaat tccaaccaag
     2101 ggaacgttca gttatcatgt gcgactttcg cggttatgaa gaaccggaaa tggtcaagaa
     2161 acgccctgtc gtcgtcatag cgcgaaacag gcacaacggc aaactggtaa cggtcgtacc
     2221 cttaagcagc acagagcctg tccctttggc ggactgccac cacaaaatga gtgaaaaccc
     2281 cttaccggac aagccgcaca tccaatgtcg ggcaaaatgc gatatgacgg caacagtcgg
     2341 attggcacga ttagaccgat acaaacccaa agggcgcgac cgttgcatcc caataatcag
     2401 tgaagaggat tttcaggcgg ttaaaacagc cgttgccaag gcattcaaac tgtactagaa
     2461 taaaaccgtt cccttaaagg ggcttgcaag actgttccga aatatgggca gccgcgcacg
     2521 ggcgacaggc gatgacaagc cgtccgtgcg tgtgatgggg cgcggaatgc gccccttgtc
     2581 gtatctgcaa acgcctacaa atccccaatc atcccttcaa taaaaatgcc gtccgaacct
     2641 tcaggcggca ttttccgttt accttgccgc agccttgccg taaatgcctt caagacatgc
     2701 ggctatgcgg ccggaggcgt ctttgtccgc atatccccgc aacacttcga gggccgcggc
     2761 ggatcttttc aggattgaac gggtttcgtg ccaaaccgtc cacatcgtaa ccgcctgttt
     2821 gcagccgagc tgcttcagcg gcgcggaaac gtctttgccc aattcgatca tccgtgtgcc
     2881 gtaataaacc atagcggcaa tgtcggccaa agcgttgccg cttatgggca gggcgggttg
     2941 cggcgcgggc aacggttcgc ggtcggggac ttcgccggcc aaacccgtgt gcagtgtcaa
     3001 tgcgtggacg taggcgacgg cttcgggcag tttgtcggcg gggatgcctt cgacggcttc
     3061 gacgttgaag cgttggtgta tcatactgta cgcggaggag tagcctatgc gtttgcgccc
     3121 gacaagcgcg gcaaccgccc ggcgcaatcc ggtacggtcg tcggcggtgg ttttgggagt
     3181 aatttggtag ccgcctgttt tgcggatttg agggataacc tcttcaaata tccagtcttg
     3241 gaatttgacg gcttcggctt tgcgtgaacg gaagatgact cggtagagat tcggctcgtt
     3301 gatgaaaagt aattcttggt tgcctttttt tgttgcaacg ctggattttt gaatgccctg
     3361 gtcttttaat ggcaactgac gggcattttg aatttgaaga atttccgcaa catcgccaag
     3421 gcaaaaccaa ggttcagccc ccaagatttg agtacggaca ttttgagatt tgaaagaaaa
     3481 tacagaaatg gtgttttgca ttttaagctc cacgaaagtt tgaaagcctc tcttcgacca
     3541 agaagaggta ggcggacgtt tcaggttggt cgactgctcg tggaaacagc acatccggag
     3601 atgtcccgaa agtccgcccg ttggcttttt cagcaagggt gtatgtttaa catacaccct
     3661 tcagggaact cttatcaaaa caaaagccgc aagcgcggca ataaccacga aagaaacagg
     3721 cgaccaaacc tgtgaaccga actttactat tattccgcta ttgcgtcaat attttggggg
     3781 gaagcacgga cggagctttg ttgaaagttt aaaacttgga cagtattcat ggtagaatga
     3841 cctttctatt tcatggtaga tagacgcgga aagagagttg ccgctctgct ttccacctgc
     3901 ccaaatcaag ttacagcttg gtttgggctt cttttttacc tgcaatcagc aagtgttgaa
     3961 attataattt gtatctacga ttcatgcaag ccatttctat tattttttca gtttttctaa
     4021 ttcaagttca tgtttcagct tttcttttac ccatttggag aagtctaaat tattggaaaa
     4081 ttccaataaa ttagcttctt tttcagtgtt aaatgaaacg tgctttaaaa cgcgcttaga
     4141 ttcgtagctt tttttggatt gtttggttgg gttcattctt cccccttgtt gaaaataatc
     4201 gtagatacat tttactcgat ttaaaatcat agggcaacaa aaaaaccgcc aagtttgcgg
     4261 cttggcggcg gtcgggctat tttaagaact cttttcggat ttgttcttta acccattggg
     4321 aaaaactaat tttatttgca ccttatttgc acctttttta taaatataat tcaaatattt
     4381 gatttatata ttaatttaat taaatattac atgaccctgc ataggggcga cggaaaggcg
     4441 gcgcggggga agggtgtgtg tattgtcgtt cattgtcgat atttcatcat tgcggactca
     4501 atttcaatcc ggaacaacct tcatttttgg ggaaatccgc gtcatgaaag agtccagcag
     4561 tatggttacc ggcccgtcgt tgcagagcga cacctgcata tgcgtgcgga aacgccctgt
     4621 ttcgacatga atcccgtgtc cgcgtaacag ctccgccgtt cgcaggtaaa gccgttgcgc
     4681 ctgttctgcc ggcgcggctt gggaaaacga aggccgccgc ccgcttgccg cgtcggcata
     4741 aagcgtaaac tgcgacacca gcagcaccgc gccgccgaca tctttcaaag acaggttcag
     4801 cttgcccgct tcgtcttcaa acacgcgcaa atgtgcgatt ttgtcggcga tatagcgtgc
     4861 atctttttct gtgtcgctat gcgttacacc gagtaacacg acaaacccgc cgtcgatttt
     4921 gccgcaggtt tccgtgccgg cttcggacac gacatccacc tttgcaccta ccgttttctg
     4981 tatgaccgca cgcataagcc gtcctcctta cctgaacgtg caatgccgtc tgaaaaggct
     5041 tcagacggca tcggtatcgg aaatgccatt acggtttgac accgttttct tccaaaaggg
     5101 catcgatttt caagcgggct ttttcctgcc tcaaaccttg ttccaactgg tttctgacca
     5161 actcgaaagg ctgcgcgtcg gggtttttcc cgaccgcgcc gagtttgaac aggtaatagc
     5221 gttcgcccaa tttgaccgga ttgcgggtaa cgtcgccacg gttcataccg gcaaactgcg
     5281 aagccagcgg ctcgggaagc tgctgcgcca taatgaaacc gtcgaacgcc tgctcgtcgt
     5341 tcggataacg cttcatcagc ccttcaaaag acagcccttt gagcaggagc tgctgcgcct
     5401 gacgcgcctc ctcttcggtt gcgaagctga cctgctgcaa tttgatcatg cggatttgcc
     5461 gctcataaaa ctgacgcagt gcgctttcgg aaaccgtttc cgaacgttcc agaaaacgga
     5521 cgtactcctc ggcataaaaa gacgcttcgg cgattttaaa gcggttttgg acatccttat
     5581 ccttatccaa accttccttc aatgccctgt ttttcaaaac ttccaaagtt tgcagccggc
     5641 ggacggcatc gtttcggatt gcctgcccgt ccggtctttg ggactgctcc gcatgccggt
     5701 ctgcctgctg catgatctgc gccaccagcg tatccaccaa agccgggtcg atttcgggtg
     5761 ctttggctgc cgcaaaacct gccaacattg cagcaataac tgcggcagcg gtctttttct
     5821 gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc tgtatgaacg tcttgccgcc
     5881 gtatcccgtt ttcagattat tttgcaggtt tgatgtttgc cttacccaac agcgcaccga
     5941 cggcacggtc aatccgttcc gcctgaaggt tgccggcaat ctgtcctttc atttcgtcaa
     6001 aagaaggcac tttcacctcg cggctgtcgt tgacataata aacgccgtag aaatcgccgt
     6061 ttttcagcgg cgttgccgta aattcgcctt ttttcaagtc cttaattgcc tgataaagcg
     6121 gcggaacacc ctgttccaaa tctttcagcg gcacatatcc gtccggcgcg ccggtccgtt
     6181 tggtgcggtc gttgagcgag tattgtttca aaacggcatc aaaacctttt ttcgccttca
     6241 aatcggcaac cgcttttttc gcattttctt ccttgtcggt caggatttcg cccaactgga
     6301 cttcctgcgt gcctttataa aaaccgctga tattgtcgta aacggctttt acttcctgct
     6361 cggaaaccgg ttgggttttg gcgatatgca gtgcgtatgc ctcgccgttc aagccatatt
     6421 ttaccgcctg ccaaacggtt ttgaaggaca gtttcttgtc gtcgcccgac tttttcgctt
     6481 cggcacgcaa tttggcaagc gcatctttaa actctgccga ccggtcgagt ttcaggcgtt
     6541 tcacttcctg tgcgaccacg gtgttgacca cttcgttttc cagcagggat tggcgcagtt
     6601 gcggcgtgtc ttcggcacgg ctgttttccg cacggaatgc ggcaacctgc gcatcgatga
     6661 cggaactgtc gattttctga ccgttaacgg ttgccagcgt ttgggcaaac aggctgccgg
     6721 aacaggcaag cagcgcaacg gaagtcagga tttttgcttt cataatcttc tctctttaaa
     6781 ttttaaattg aaatattcag gcagacttca ttcagtccgc cgtatggaaa tactcgtcag
     6841 gggtagccgc ccggatgccg agcgcgtgaa tcatgccgcc tgaaaataaa tctttgagca
     6901 gcgatttgac cgttttctga cggttcagac ggcttaaacc ttcaaaacgg ccgctgacga
     6961 ccaaaacggc ataatgtccg ccgccggtat tgcccgcgtg tcctttgtgc agatggctct
     7021 catcgccgat ttccaacacc agaggatcga gcgtctgcag gcgttcgcgg atcaaatcga
     7081 cagccggcat tatttgaaca ccgctttaaa cggcttgatc agcacttcgc tgtacacgcc
     7141 ggcatgaaca tagggatcgt cttcagccca agcctgcgcc gcatccaaag actcgaactg
     7201 tgccacaatc aagctgcccg aaacacgttc gggattgtcc ggcagcaggt ttgggcctgc
     7261 cgtcagcaga cggccttccg atttcagtgt ttccagccgt ttaaagtgtt cgggacgtgc
     7321 cgccatacgc gcttcatgca catcctcccc gtctgttgcc agcaacataa aatattccac
     7381 agtcaatcct cctttttcag ataggtactc agataaatac cctgaataat aaagaaaaaa
     7441 agcatcagca cagtcgaacc gaacatctta tagttaaccc attgcgcttc aaacctagta
     7501 aacacaaacc agttggcaat acccataaaa atcagaaaac cgacccacat atatgtcaat
     7561 tttccccata cggcatccgg aagctgaatc tccctgccga tactcgcttt caagccgttt
     7621 ttacccgcaa ggtggctgcc cagcaggaat aacgccccgc accagaacaa tactgtcggc
     7681 ttccacataa tgaagcggct gtcgcccaaa acaatggttg cgccgccgaa tacgacaatc
     7741 agcaccagtc cgacccactg catcgtatcc agccttttat gcttccaata caggaaagcc
     7801 gcctgaacca cgcctgcaac caaggcaacc gccgcagcgg caatcatatt tttggtaacg
     7861 gtataagtag caaaaaacaa gatgacggac aaaaggtcgc tgacaaattt catatccaaa
     7921 tgagaaagca aatcaaataa acgctaatca tacaggcaga accctatctt tgcacctgca
     7981 aaaccaacaa attttgtttt gattaatgca aatatgcaca gatgtttttg aaaaaagatg
     8041 gaaatatgtc ataatttata aaattggctt taaaatacag cttcggccgg aacctctgcc
     8101 tatccgcacc ctatcgtccg tattgcctat gcctgatgac gaaatttaca gactgtacgc
     8161 ggtcaaaccg tattcagccg ccaacccaca ggggatacat cttgcaaaaa caacagacaa
     8221 atcaaactga ttgccgcctc catcgcagtt gccgcatcct ttcaggcaca tgcgcgactg
     8281 ggcggactga atatccggtc caaccttgac gaaccctttt ccggcagcat taccgtaacc
     8341 ggcgaagaag ccaaagccct gctaggcggc ggcagcgtta ccgtttccga aaaaggcctg
     8401 accgccaaag tccacaagtt ggacggcaaa gccgtcattg ccgtttcttc cgcacaggca
     8461 gtccgcgatc ccgtcttggt gttccgcatc ggtgcaggcg cgcaggtacg cgaatacacc
     8521 gccatcctcg atcctgtcgg ctactcgccc aaaaccaaat ctgcactttc agacggcaag
     8581 acacaccgca aaaccgctcc gaaagcagag tcccaagaaa atcaaaacgc caaagccctc
     8641 cgcaaaaccg ataaaaaaga cagcgcaaac gcagccgtca aaccggcgca taacggcaaa
     8701 acccataccg tccgcaaagg cgaaacgctc aaacagattg ccgccgccat ccgtccgaaa
     8761 cacctgacgc tcgaacaggt tgccgatgtg ctgctgaagg caaacccaaa tgtttccgca
     8821 cacggcagac tgcgtgcggg cagcgtgctt cacattccga atctgaacag gatcaaagcg
     8881 gcagcaccca aaaaaatcaa agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc
     8941 gaaactgcat ccatgccgtc tgaaccgtcc aaacaggcaa cggtagagaa accgatagag
     9001 aaacctgtcg aaaaaccggt tgaaaaacct gaagcaaaag ttgccgcgcc cgaagcaaaa
     9061 gcggaaaaac cggccgttcg acccgaaccc aaacccgcag tgtccgaaac tccggcttcg
     9121 gcgacagaac gccaacccgg acctgtaccc gctgcaaata ctgccgcatc ggaaaccgct
     9181 gccgaatccg cccccccaag aagccgccgc ttctgccatc gacacgccga ccgacgaaac
     9241 cggtaacacc gtttccgaac ctgttgcgga aacagccagt gaagttgccg ccgaacccgt
     9301 tgccgaacct gtcgaacagg tttctgccga agaagaaacc gaaagcggac tgtttgacgg
     9361 tctgttcggc ggttcgtaca ccttgctgct tgccggcgga ggcacggcat tgatcgccct
     9421 gctgctgctt ttacgccttg cccaatccaa acgcgcgcgc cgtaccgaag aatccgtccc
     9481 tgaagaagag cctgaccttg acgacgcggc agacgacggc ataaaaatca cctttgccga
     9541 agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac gatgtaaacg acacacttgc
     9601 cttaggtggg gaatctgaag aagagttatc ggcaaaacaa acgttcgatg tcgaaaccga
     9661 tacgccttcc aaccgcatcg acttggattt cgacagcctg gcagccgcgc aaaacggcat
     9721 tttgtccggc gcacttacgc aggatgaaga aacccaaaaa cgcgcggatg ccgattggaa
     9781 cgccatcgaa tccacagaca gcgtgtacga gcctgagacc ttcaacccgt acaaccctgt
     9841 cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc caaactgccg aaaacaaacc
     9901 ggaaaccgtc gataccgatt tctacaacaa cctgttctca aacaaccata tcggcacaga
     9961 agaaacagct tccgcaaaac ctgccgcacc ctccggactg gcaggcttcc tgaaggcttc
    10021 ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc caaacaccgg aagagttgca
    10081 cgatttcctg aaagtgtacg aaaccggtgc cgtcgcggaa actgcgcctg aaacgcccga
    10141 tttcaacgcc gccgcagacg atttgtccgc attgcttcaa cctgccgaag caccggccgt
    10201 tgaggaaaat gcagcggaaa tcactttgga aacgcctgat tccaacacct ctgaggcaga
    10261 cgctttgccc gacttcctga aagacggcga ggaggaaacg gtagattgga gcatctacct
    10321 ctcggaagaa aatatcccaa ataatgcaga taccggtttc ccttcggaat ctgtaggttc
    10381 tgacgcgcct tccgaagcga aatacgacct tgccgaaatg tatctcgaaa tcggcgaccg
    10441 cgatgccgct gccgagacag tgcagaaatt gctggaagaa gcagaaggcg acgtactcaa
    10501 acgtgcccaa gcattggcgc aggaattggg tatttgattc ccaactgccc tttcgcagat
    10561 caaggatgcc gtttcagacg gcatcttttt ttgccttatc ggtgtaacgg ataaagtttg
    10621 aaccggcaca ggctcaaaca gcaggccgtc ggcaacaaaa tgccgtccga aacccctaaa
    10681 ggcttcagac ggcattggcg gcggcggtat caggtattcg cataggcaac cgaatcgtcg
    10741 ccgcttttct tttgaacgaa ctcgattttg catccgtcgg ggtcttcgac gaaggctatc
    10801 acggttgtgc cgtgtttcat caggccggct tcgcggacga cgtttccgcc ctgccgcttc
    10861 acgcgttcgc aggcttcgta ggcatcgtcc acttcaaccg cgatgtgtcc gtaggcgtcg
    10921 cccaagtcgt atcgttccgt atcccagttg tgcgtcagtt ccaaaaccgt gctgtcggtt
    10981 tcgtcgccgt aaccgacgaa ggcaagggta aatctgcctt cgggatagtc ttttcggcgg
    11041 agcagtttca tacccaaaac gttttggtag gaatcgaggg atttttcgag attgcccacg
    11101 cggagcatag tatggagtaa gcgcattttt cgtgttcctc tcggtggtgg ttaaacttcg
    11161 attttattcg gcgtaaacgt ctgccatttg ttgcaggcgg gacagtgcca gaaaaagact
    11221 tgggatttga agtggcagtt gcggcaacgg tacatcacgc tgcgctggag ctgccgtccg
    11281 ataaccgaac gcatcatgtc ggcatcggct ttccaagccg gatccaaatc gctgagtttc
    11341 aaaccgagca ggcggtacac gccgttaagg tcgggcttgc ggcggacaag ctcgacggcg
    11401 gtttgcgcgg cttctttctc gcccttaagc agcagggatt tctcgtacac gacattgatc
    11461 aggtcaagtt cgggaaacgt ctgcatatat cctgtcagac ggttcaagcc ttcttcaggt
    11521 tttccctgcg cggcataggc ttcgtaaagc ttctcgccga ccatgctcaa gtatgcatgg
    11581 ttttgctgct cgatggcggc ataggcttcg acggcggcag ggaaattgcc ttgtcggtgt
    11641 tcaatgtcgc ccaaaatcat gttggcgcgg gtgcattttt tgttggcttc gagtgccttg
    11701 ccgacattga aacgcgcggc atcgaaattg gacttgaaca gcgcggcttg ggcaagttcg
    11761 caataaaact gtgcaatctc aaactgatat gtctgttcgt cgtgactaag aagttgggcg
    11821 gtttcaaccg ctttttccca atccctgtcc tgctggtaga tattgagcag gtgctgtctg
    11881 gcttcacgcg ccatttcacc gtcttgcagc cccaaaaaaa tctgttcggc acgatcgacc
    11941 aaacccgcgc tttggtagtt ttgcgccaat tcaaacagga cgcgcgcgcg cttttcgccg
    12001 accgtatcgg gagaatcgag cattgtccgg tgtatgttga tggctttgtc gttttcgccg
    12061 cgctgacggt aaagtttgcc gagggtgagg ttcaaatcat acgattgcgg ccggccgtcg
    12121 acgacttccg ccaactccct tgccgcgcgc ccgctgttgc ggtcgaccaa agcgtccagg
    12181 cttttataaa atcccgaagg gatgcttttt gcctgcttca atacggtttt catatccacg
    12241 cgggcggcaa accagcccat cgtgaagaag acgggcaaaa ggataatcgg cagcaggata
    12301 atccacaatt cgttgtccat atcggctttc ttaaggctgt ttggcagatt cggcagcatt
    12361 ttgtatcggc ggtgcagtca atttctgtcc gctcaagcgc gcacttttct tcacttccgc
    12421 acgcaggcgg ctgttttcgc cgcgcaagga cagcagccgc ccgaacaggg caaacattcc
    12481 gaacacgatg ccgacgacaa acgcgccgaa caatacgaca atcagcggca gattgacact
    12541 ctgccccgga agataggaaa aggtaacggc atccatatta atgacggcaa gcagcaggaa
    12601 gagcagcagg ataatgattt tgatgacggt atagataagt ttcatgtttg ctccgtaaat
    12661 aaaaacgccg cacggacagc ttcgcactct gaaccaagat tgcgttagtt taaacgattt
    12721 gcacggtttc ggatagaatg cggcagcgtg ttcggacggc atacggagta tgcgattgcc
    12781 gacaatttta ccaaatacac cgctcctttc catttgaaaa ataacggatt ggacaccgca
    12841 tcgacagaaa aacccgccgc gcacttgtca aaaccctgtt tgcaggcgta tctttacaac
    12901 cttcaaattc aaaccgttca ttgaaacata tcagaataag aaaggcttta catcatgagc
    12961 agacccgtac ccgccgtatt cggcagcgtt tttcacagtc aaatgcccgt cctcgcctac
    13021 cgcgaaggca aatggcagcc gaccgaatgg caatcttccc aagaccttac ccttgcaccc
    13081 ggcgcgcatg ccctgcatta cggcagcgaa tgctttgaag gtttgaaggc cttccgtcag
    13141 gcagacggta aaatcgtgct gttccgtccg accgccaata tcgcgcgtat gcggcaaagt
    13201 gcggacattt tgcacctgcc gcgccccgaa acccaagctt atctcgatgc tttggtcgaa
    13261 ctggtcaaac gtgccgccga cgaaattccc gatgcgcctg ccgcgctgta cctgcgcccg
    13321 accttaatcg gtaccgatcc cgttatcggc aaggccggtt ctccttccga aaccgccctg
    13381 ctgtatattt tggcttcccc cgtcggcgac tatttcaaag tcggctcgcc cgtgaaaatc
    13441 ttggtggaaa ccgaacacat ccgctgcgcc ccgcatatgg gccgcgtcaa atgcggcggc
    13501 aactacgctt ccgccatgca ctggttgctg aaggcgaaag ccgaatacgg cgcaaatcaa
    13561 gtcctgttct gcccgaacgg cgacgttcag gaaaccggcg catccaactt tatcctgatt
    13621 aacggcgatg aaatcattac caaaccgttg acagacgaat tcctgcacgg cgttacccgc
    13681 gattccgtgc tgactgtcgc caaagatttg ggctataccg tcagcgaacg taatttcacg
    13741 gttgacgaac tgaaagctgc tgtggaaaac ggtgcggaag cgattttgac cggtacggcc
    13801 gccgtcatct cgcccgttac ttccttcgtc atcggcggca aagaaatcga agtgaaaaac
    13861 caagaacgcg gctatgccat ccgtaaggcg attaccgaca tccagtacgg tttggcggaa
    13921 gacaaatacg gctggctggt cgaagtgtgc tgatgcttta aataaaaaaa tgccgtctga
    13981 aacccgtttg gcgtttcgga cggcattttc gcgttcggac cgtttccgcc gcacctgcag
    14041 caagtcggta caaaggcaat cggttaaaac aagcgtccgc atttcccatc ccgcctgccg
    14101 caagtcgggc atttccctgg aaatacgctt caaacggcaa accgccgccc gaaaccgata
    14161 ctcggcacgg acggcgcacg gatttgaaaa cggcggatta tccctcggtg ctcaaggcat
    14221 tgatgctgta accgccgtca acataagtga tttcgccggt aatgccggac gacaggtcgg
    14281 acagcaggaa ggcggcagta ttcccgactt cttcgatggt aacgttgcgg cgcagcgggt
    14341 tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc gatgccggag gcggcgagcg
    14401 ttttgatcgg gccggcggaa atgccgttgc agcggatgcc ctctttaccc agacaggcgg
    14461 cggtaaagcg gatgcctgcc tcaaggctgg cttttgccat acccatcacg ttgtaattcg
    14521 gaatcgcgcg taccgcgccc aagtagctaa gggcgacgat ggcggagttt ctgccgcgca
    14581 tcatcggacg ggcggctttt gccaatgcgg gcaggctgta tgcggaaatt tcgtgtgcgg
    14641 tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc gctcaaggct tctttcggcg
    14701 cgaagccgat ggaatgcacc aaaccgtcca agccatccca atgtttgcct aagtcggcga
    14761 acacttggtt gatttcgtcg tcgctggcga catcgcagcg gaatacgagt tcggaatcta
    14821 attccgccgc cattttgcgg acgcgctctt ccagtttgtc cacaacgtag gtaaacgcca
    14881 gttccgcgcc ctgttcgcgg caggctttgg cgatgccgta agcgatggaa cgctcggaaa
    14941 tcatgccggt aatcagaatt tttttgcctt gcagaaaacc cattttgtat ccttcaaata
    15001 gtgttcggtt cgttctaaac cgttgattat agcaaattgt ccctgtttct gtgttttcac
    15061 gttgcagcgt gcagacggca atgccgtccg aagcggattt cagacggcat tggacgtttc
    15121 aaatacggtt taaggcatca gatgccgcgc aagagttcgt tcacgctggt tttggcgcgg
    15181 gtttgcgcgt ccacgcgttt gacgatgacg gcacagtaaa ggctgtggct gccgtctttg
    15241 gaaggcaggc tgccggacac gacgaccgaa cctgccggta cgcggccttg gtagatttcg
    15301 cctgtggtgc ggtcgaagat tttggtggat tgaccgatga acacgcccat cgaaatcacg
    15361 ctgccttctt cgacaatcgc gccttcgacg atttcggaac gcgcgccgat gaagcagttg
    15421 tcttcaatga tggtgggtgc ggcctgcagg ggctcgagta cgccgccgat gccgacaccg
    15481 ccgctcaagt ggacgttttt gccgatttgc gcgcaagagc cgacggtcgc ccaagtatcg
    15541 accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt aagacggcat cagcacggcg
    15601 tttttggcaa caaagctgcc gcgtcgggca accgcacccg gaactgcgcg gaagcctgcg
    15661 tttttgaact cgtcttcaga ccagtcggca aatttggtcg gcactttgtc gaagtatttg
    15721 ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga aggacagcaa cacggctttt
    15781 ttcgcccatt cgttgacttt ccactcgccc acgcccaagc gttcggcgac gcgcagtttg
    15841 cctgaatcga gttggcggat ggtttccaac acggcttctt tgacttcggg ggcaacggtg
    15901 gtcggagtga tgtccgcgcg gttttcaaag gcggtttcga taatgttttg caaagacata
    15961 atgtttcctt ggttcgagat atttcgggac gcttgccggt attgttcaga cggcatggcg
    16021 gttggaacaa acgtgctatt gtgccataca aacgcccgcc cgtcagccgc gccgcaggct
    16081 tgccggatgc cggcgtttcg gaatcgggcg ggcggcaggc atatgtcttt ttccgcgccc
    16141 gaatcaatac agaaaggcgg cggtactttt atgccgcgcc gccgcctttc agacggcatt
    16201 cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg tacggacgtg ccgccttcca
    16261 attcgccgat gatggttttt gccaactgtt tgccgtactc aaccccccac tgatcgaagg
    16321 ggttgacgtt ccatatcgcg ccttggacga aggttttgtg ttcgtaagcc gccatcagca
    16381 tacccagatt gcagggcgtg aggcggtcga gcaggatgct gttgctgggg cggttgccgg
    16441 ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc gggcaaatct gccagttcgg
    16501 cgcgtgcttc gtctaaggtt ttacccttca tcagggcttc cgcttgggca aaggcgttgg
    16561 caacggtaaa acggctgcgt ccgtcctccg cgccctgcgc cgtcatcggg acgataaagt
    16621 cgcagggaat caggcgcgtg ccttggtgga gcagttggaa ataggcgtgc tggcagttga
    16681 ccccttcacc gccgaacacg atgccgcccg ttttgcacgc ggcgggactg ccgtctgaag
    16741 cgcggctttt gcccaaactc tccatatcga gctggttcag ccacgccggc agcaggcgca
    16801 ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg ctggaaattg ttataccaca
    16861 cggcaatcag tgccatcaga acgggaatgt tgcgacgcgg gggcgtgtgg aaaaaatggc
    16921 tgtccatcgc gtgcgccccc gctagcagtt cgcggaaacg cgccccgcca accgcaacca
    16981 tcacgggcaa accgacgggc gaccagacgg aatagcgtcc gcccacccag tcgtacatcg
    17041 caaacacgcg ttccgccgca atgccgaaac tttgcgccgc ttccgtatcg gcagacaccg
    17101 cgcaaaaatg gcgcgccgtt tcggattccg agaaccccgc accgcgatac cacgccttga
    17161 ccgcctctgc attgagcagg gtttccggtg ttttaaagga cttgctggca acgcaaaaca
    17221 ttgttgtttc ggggttcaga cggcataaga cctcatccag gcaggcaggg tcggcattag
    17281 aaacaaaatg gacggaaatc tgccgtctga acggctcgag cgcctgcacg cacattgccg
    17341 gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc cgcaatccgt tttccggtta
    17401 tcccctgata taaaccgtcg tccaaactgt gtgcaaactt caacgcacga tttaactcgc
    17461 ggcggatttc gggcaacacg tccctgccgt ccgcataaac ggcatccgca ccgtcgggca
    17521 ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc gttgactttc gcacccgtcc
    17581 gcaaagcacg cattttccct tccaaatccg ccgtctccgc aagacggcag agcagttgca
    17641 gcgtatcttc gccaaaacgg tttttgctgt aatcgaacaa catcccgtcc aaacgctcat
    17701 gcatacgctc aaaacggtcg ggttcggcag caaagcggtc gcgcaaaagg atgtggcacg
    17761 tatcctgata atggcgttcg agcgcatacc atgcacgggt aaaagcatcc atctgttttc
    17821 cttgattttt cagaaccgga ttaaaatata gcagaatgta gtctaacaaa cggcagacac
    17881 tttagcgaat ctcaggaaca ccgcccgccg cgtccaataa caccttattt ccatatggcg
    17941 aaaatatagt gaattaacaa aaatcaggac aaggcggcgg gccgcaggca gtacggatag
    18001 tacggaaccg gttcgcttgg tgcttcatca ccttagggaa tcgtcctctt tgagccgggg
    18061 cgggccaacg ccgtaccggt ttttgttaat tcactatacc ctccggccgg catttgaccc
    18121 gcaacaatat cctgcaagat ttattgtgtg cgcataaatg ccggacagcc gcctaaatta
    18181 aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg tcggcgattt
    18241 tcatcttacc gggaacacgc cgatactgtt ttttaacgat gtttcttaca ttttattcca
    18301 attactttac ggggctagaa tatggctaaa aatgggggat tttctttgtt cgcaaagaaa
    18361 gaaaaacgct ttatctttga aggcaggcat tctgcctccg acaaactggt caacggcgaa
    18421 gtatccgcgt ttaccgaaga agaggcacgc aaaaaactgg caaaacgcgg catccgcccg
    18481 ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga agacattacc
    18541 gttttcaccc gccagctttc cacgatgatt aaagcgggcc tgccgctgat gcaggcattt
    18601 gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat ggaaatccga
    18661 ggccaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc gaaatatttc
    18721 gaccgcttct actgcaacct ggttgcggcg ggcgaaacgg gcggtgtatt ggaaagcctg
    18781 ctggacaaat tggcaattta caaggaaaaa acccaggcca tccgcaaaaa ggtaaaaacc
    18841 gcgctaacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt cgtgatgatg
    18901 attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga gcttccccca
    18961 ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg gatggtgctg
    19021 atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc gattaaaatc
    19081 caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat tgtccgcaaa
    19141 gggacgattg cccgctgggg caggacgacg gcaacgctgt ttgccgccgg cgtgcctttg
    19201 gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga agaagccacc
    19261 cgggaaatcc gtacgcgcgt catccaaggt ctgtctatga cttcggggat gcgtgcgacg
    19321 gaactgttcc ccaatatgat gttgcagatg tcctccatcg gcgaggaatc gggttctttg
    19381 gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa tgcggtcggc
    19441 aggctgtccg ctatgatgga gccgattatt attgtgattt tgggcttggt catcggtacg
    19501 cttctggtcg ctatgtatct gccgctgttc aacttgggca acgtggtcgc ctgatttgcc
    19561 gcacagatcc ggcgcggatt ggttctgcgc cggtttgttt ttgctttgaa tatatcaagg
    19621 acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg cagcagtttt
    19681 ggggctgctg gtcggcagct tcctgaatgt cgtcatttac cgcgtacccg ttatgatgga
    19741 acgcggctgg acggtatttg ccaaagaaca tttaaacctg ccgctgaccg acgatgaaag
    19801 ccgtaccttc aacctgatga agccggattc ctgctgtccc aaatgccgtg tgccgatacg
    19861 cgcgtggcag aacatcccga ttgtcagtta cctgctcctg cgcggcaaat gcgcttcctg
    19921 ccaaaccaaa atcagcatac gttatccctt aatcgagctg ctgaccggcg tattgttcgg
    19981 gctggtcgcc tggcaatacg gctggtcttg gattacgctg ggcggtttga tactgaccgc
    20041 gtttctgatt tccctgacct ttatcgatgc ggacacccaa tacctgcccg actcgatgac
    20101 attacccttg atctggctgg ggctgatatt taatttggac ggcggcttcg tgcctttgca
    20161 gtctgccgtt ttaggtgcgg ttgccggcta tagttcatta tggctcttat gtgcagtgta
    20221 taaactgctc acaggaaaaa ccggtatggg caacggagat ttcaaactga ttgccgcatt
    20281 gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct ctctgatcgg
    20341 tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcgg cattttgcct tcggccccgc
    20401 actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc gggcggtcaa
    20461 ctggtggctg acccatccgg tgagatgaca gcatgggtcg gactgaccgg cggaatcggc
    20521 agcggcaaat cggcagccgc gcaatatttt gccgatttgg gcgtgccgcg catcgatgcg
    20581 gacgcggcgg cgcactcgct gacggcttca gacggcatcg ccctgccgga aatcaggcgg
    20641 ctgttcggcg acaccgtttt cgacacacag ggtttgttgc ggcgcgacat attgcgtaaa
    20701 gaaatctttg cctccccatc gcgcaaagcc ttgctcgaat ccgtgatgtt gccgctgatt
    20761 ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg cggtttacgg cattgtcgaa
    20821 attccgctgc tgacggagaa gcgtcaattt atcagcctga tacggcgtgt cctgaccata
    20881 agcgcacctt tggaaaaacg tatcggcagg gtaatggcac gcagcgggct gacgcgcggc
    20941 gaggtcgcgg acatcatcag ccatcaggca tccgaatccg aacgcctgct gcttgcagac
    21001 gatgtactgc tcaatgacgg cagcctcaaa agcctacgtg agaaaacaat gctcctgcac
    21061 gcgttttatt cagggatttt cgcctcaaaa ccaacacaag gaaaacacaa tggctgaatc
    21121 gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa acggcagtag tatggaaacc
    21181 ggaaaacgca ttccgcccgt tctgttcgca acgctgcaaa ctgatcgact tgggcggatg
    21241 ggcagacggg aaatatacgg tttccggcca aacggaaagt ttgccggaaa tatccgaacc
    21301 cgacggggca taccgctgac cgccccgcct tcccggcaaa caccctgaaa ggtcaaatgc
    21361 cgtctgaaac aaacacgctt cagacggcat tttcattctc aaacctaatc gttggtattt
    21421 gccgttacct cttccaatga agtaatgccc tgcataattt tcaaaatacc ggcccggcgc
    21481 aaatccacca taccctcctt ataggcaacg tccaaaatac ccacttccgt accgttgttc
    21541 ataatcacac gctgcatttc ttcgctgatg ggcataacct catacacgcc cgcacgcccc
    21601 ttataaccct gcccccggca acggtcgcaa ccgacggcgc ggtaaagttt ccaatctttt
    21661 gcaagatcct catcggtgaa accgacttcc ttcaaagcag aggcagacgg gcgttccact
    21721 tcctgtttgc agctcgaaca cagcctgcgt aaaagacgct gcgccataat caggctgacc
    21781 gaactggcaa tattaaacgg cgcgacaccc atattcagca tacgcgacaa cgtcgccggc
    21841 gcattattcg tgtgcagtgt ggaaaacacc atatgccctg tttgtgccgc cttaatcgca
    21901 atatcggcag tttccaaatc acgaatctca ccgaccataa tgatgtccgg gtcctgacgc
    21961 aggaaagact tcaaagcagc ggcaaaagtc agaccctgct tatcattgac gttaacctga
    22021 ttgatgcccg gcaggttaat ctcggcaggg tcttccgccg ttgcaatatt taccgactcc
    22081 gtattcaaaa tattcaaaca ggtatagagc gacaccgtct tacccgaacc cgtcgggccg
    22141 gttaccagca ccatcccgta aggacggtga atcgcttcca acaacaattt tttctggaac
    22201 ggctcaaaac cgagctggtc gatgttcaaa gacgcggcat cggaattcaa aatccgcatc
    22261 acgacctttt cgccaaacag cgtcggcaat gtgctgacac ggaaatcgac aggcttgccg
    22321 cccttttgaa aggtcagctg catcctaccg tcctgcggta tccgtttttc ggaaatgtcc
    22381 aaacgcgaca ttaccttaat ccgggaagca agctgccccc ttaccgcaat gggcggctga
    22441 accacctcgc ggagctgccc gtccacacgg aaacggatac gcgcattgtg ttcgtaaaac
    22501 tcgaaatgga tgtcggatgc cccgctacgc aaggcatccg acaaagtttt atggataaac
    22561 ctcggaacag ggccgtcttc tgcctcctcg ttgtcgatat acagggtgtg gctttcctct
    22621 tcctcttgcc cctccccaag ctcctgaagc agcgatgtcg aacgcgaacc cacccaatcg
    22681 agcaaacccg ccaactggtc atcctcgaca atgaccaact caaccgcaat ccctgcggca
    22741 gaaacggttt tctgaatttg cggcatctgg gtcggatcgg aaaccgcaaa aaatactttg
    22801 tcgccccgac ggaaaaccgg cacacagtgg aactccacca tctgctcctc cgtcaacacc
    22861 cccatcagca ccctgtggcg cggataatga cgcaaatcaa gaatcgaata actgaacacc
    22921 ctcgcaatca atgccgcaag cgacttgggc gaaatgacac cgtctgaaaa cagcatcggc
    22981 aacacttcct tacccgcctg cgactcattg tagtaatgct cggcctgctc aacagtaacc
    23041 acctggtttt gaaccagaat cctcagcaaa cctacgctca tacgacctta tccccaaatt
    23101 tattcattgt tatacctgta cagattttat cataaaacaa cagatttcca agcctgaaca
    23161 tcttttacga agcctgaaaa tcatttcacg attctgccgt cctaaaggtc ggtttttcaa
    23221 gcaggaagaa aaagttttca gatggcaaaa aagccctcca gcactgaaag gccttatatc
    23281 ggaaacttcc cgcaacacgg gaaacagaca aatgcaaacc gtcaaacctc gccaacagga
    23341 atcgaacctg tattttacgc ttaggaggca tacgttctat ccgttgaact atggcgagcc
    23401 gaaatgaaaa gggagatttt aaacctttcc ggcaacaaaa gacaagatgt cggccgcaat
    23461 cagcggcccg acagggatac cgcccataaa tgccaccccg atgaccgtac cgatcagcaa
    23521 gcccgttacc aataccggct gccgtcccat caaaggcacg ccgcatcccg ccagccaagc
    23581 cacgaaaata ccgataaaaa cggcggatat cattttaaaa ttcaaaaatt cggcaacagg
    23641 aggaacctgc gcctttcctg aaaccaacgg actcaaaacc cctatggtca aaagaatgat
    23701 accgaggttc aacccgtgct tctcgaccaa cgggacaaac tgtaccaatg ccgtctgctg
    23761 catcagcagc aatatggttg ccgagacggt aatcgaattg ttgttgctga ccacccccaa
    23821 cagaatcagc gtaaccagaa acaggggaac aaaactgaaa ttcatcggtt acgacaccct
    23881 gaccaaagat tccttcgcca gctgaatcat agcatccttc acttcgctgt cggacagggc
    23941 atccaacgcg gcaacggcac aatcgactgc tttgcgcgcc tcgcctatcg aatatgccaa
    24001 agcatccgaa cggacgacat aatcgcggat tttttcaaaa tagccgcgat ctgcattttc
    24061 caaagcagta cgcacatcgt tcgcaacctg ttcggaaccc tgacgcatca gataaatcaa
    24121 aggcagggtc ggttttcctt ccgccaaatc gtcgccgacg tttttacctg tttcttcggt
    24181 ttcccccgaa taatccagca catcgtcaat aatctggaat gccgtaccga cgtacatacc
    24241 gtagtctttc aaggcctgtt cgtgcccggg ggaagctttg cccaaaattg cgccgacttg
    24301 agcggcagct tcaaacaatt ttgccgtttt atattggatg actcggatat attgttcttc
    24361 ggtaatgtcc gtattgccga tgttcatcag ctgcatgacc tctccctcgg caatgatgtt
    24421 ggtcgcatcc gccatcactt ccaaaacgcg catactgccc gaggcaacca tcagttgaaa
    24481 tgcacgggta tataaaaagt cgccaaccaa cactgccgcc gcattgccga acagattgtt
    24541 tgccgttgcc cgcccacggc gcaaatcgct ttcatcgacg acatcgtcgt gcaggagtgt
    24601 ggaagtgtgg ataaactcga ccatcgctgc cagcgaatac agtttctcgt catcataacc
    24661 gaccgcctta cccgccaaaa tcgtcataat cggacgcagg cgtttgccgc ccgcgctgat
    24721 gatatatgta ccgatttgcg aaatcagtgc gacatcggat tgcaccgcac ggttgatgac
    24781 ttcattgact ttggcaaggt cttcaggcag atgtcgctgg aaatagggca gattctcgag
    24841 catggcagac tctcaggttt acgtttccgt caaacggcag ataccgcttg aaagctgatt
    24901 caaatagcgg gcaattatat cagcaaagcc atcagggtac atcttttcag acggcatatg
    24961 attgcccgaa ccgcgcaaac tttgacaaaa caggcaaata acaatacaat gcccaatttc
    25021 gcacaatggt gtgcggatat taaccataac ccttatggag ttgagtatgt acgcggtcgt
    25081 aaaaaccggc ggcaaacagt ataaagtttc cgtcggcgaa aaattgaaag tagaacagat
    25141 accagcccaa ctcgacagcc aaatcgaact gaccgaagtt ttgatgattg ctgacggcga
    25201 atctgtaaaa gttggcgcac cctttatcga aggtgcaaaa gtaacggcta aagtagtggc
    25261 acacggtcgt ggcgaaaaag tccgcatctt caaaatgcgc cgccgcaaac actaccaaaa
    25321 acgccaaggc caccgccaaa atttcaccca aatcgaaatc gtggcaatcg cctaacctta
    25381 aaacttagga gcattacaaa tggcaagtaa aaaagcaggc ggcagcaccc gcaacggtcg
    25441 cgattcagaa gccaaacgct tgggcgttaa agcctacggc aacgagctga ttcccgcagg
    25501 ttccatcatc gtacgccaac gcggtaccaa attccacgca ggcgacaacg taggtatggg
    25561 caaagaccac actttgttcg ccaaaattga cggttatgtc gaattcaaaa ccaaaggcgc
    25621 gctgaaccgt aaaactgtca gcatccgtcc ttacaccggt tctgaagaat aattcaaccg
    25681 atacctgaag ccgcatcttt tcacgatgcg gctttttaca taccccatac ccgatttgat
    25741 acacattaag ctgaaagtaa aaatccgcat acaccctccc ctgcatattt cttcaacaac
    25801 gggttttgat ataatcgcct atctgttaca gatagttcaa acggcattca aacccttaca
    25861 aatgccgtct gaaatcatcg gcatccgcct atgcaaagga tattttatga gtttacacag
    25921 cgacatcctc gtcgtcggcg caggcccggc gggtttaagt tttgcagccg aacttgccgg
    25981 cagcggtttg aaagttactc tgatcgaacg cagcccactg actgttctgc aaaatcctcc
    26041 ctatgacgga cgcgaaatcg ccctgaccca tttttcccgc gaaatcatgc agcgtttggg
    26101 tatgtgggac aaaattcccg aaaacgaaat ctaccccttg cgcgatgcca aagtgctgaa
    26161 cggacgttcc gactaccagc tccactttcc ccaaccgact gaagcgcgcg gcgagcctgc
    26221 cgactgtttg ggctatctga tttccaacca caatatccgc cgcgccgcct atgaagtcgt
    26281 atcacaactc gacaatgtca gcattctgac cgatacggcc gttaaagaag tcaaaacatc
    26341 tgataatgaa gcgcaagtct ttttggaaaa tggagacatt ctaaccgcgc gtctcctttt
    26401 ggcagcagac agccgtttct cacaaacccg ccgacaactc ggtatttctt cagatatgca
    26461 cgactacagc cgaaccatgt tcgtctgccg catgaaacat accctttcca accaacatac
    26521 cgcttacgaa tgcttccatt acggacgcac catcgcactg ctgccactgg aaaagcgcct
    26581 gaccaatacc gtcattacag tcgataccga taaaatcaac agcgttcaaa acctttcgcc
    26641 cgaagaattg gcggcaagcg taaaagaaca gctcaagggc aggctcggcg acatggaact
    26701 ggtcagcagc atccaccatt acccccttgt cggcatgatt gccaaacgtt tctacggcaa
    26761 acgcagcgcg ctgatcggcg atgcggctgt cggtatgcac cccgtaacgg cacacggctt
    26821 caacctcggg ctttccagcg cggatattct ggcaaaactg atactcgaag ccgaacagcg
    26881 cggtcaagac atcggcgcgg cttccttgtt ggaaaaatac agcagcaaac atatgctgca
    26941 cgcccatccg ctttatcacg gcaccaatat gatgctgaaa ctgtttacca acgaaaccgc
    27001 gccggcaaaa ctgttgcgcg gtttggtatt acgagccggc aacaacttcc cgccgctgaa
    27061 aaagctgatt accaaacaac tgaccggttg aaaactgccg aaatgccgtc tgaaacgttt
    27121 cagacggcat ttttgtatta tgaaccgttt tgcggtttcc atatccttgg cagacggttt
    27181 gcagcagatt cgcatacggc agatgtttca agcagaaagg aaacacaata aaaataaaaa
    27241 agcctccgaa cagtcggagg ctttgctttc aaactgaaat tatttcagtt tggtttcttt
    27301 gtacactacg tgtttgcggg caaccggatc gaattttttg atttccagtt tgccgggcat
    27361 agtgcgttta tttttggtag tggtgtagaa gtggccagta cctgcaccgg attccagttt
    27421 gattttatcg cgcattgcag taatccttaa ttaaatagtg tttaaattaa gcttcgccgc
    27481 gagcacgcaa atcagccaat acgacatcaa tgcctacttt gtcgatggta cgcaatgcag
    27541 cgttggaaac gcgcaggcga acccagcggt tttcactttc tacccaaaaa cgacgtgatt
    27601 gcaagttggg caaaaaacgg cgtttggttt tgttgttggc gtgcgatacg ttgttgccgg
    27661 acatcgggcg tttaccggtc actttgcaaa ctcgtgccat aatcagtctc caaacttcta
    27721 aaatagtaaa acgcgattta taccacgaaa aacaatgccg tttcaagtgt tttaaagaaa
    27781 aacagatgtt ttgcgcgggt aagccgcccg agtgccgcat ctgcggaacg tttgcaggtg
    27841 cgaaacagat ggggcgcgat tatagcggat ttcgggagct tctgcattca aaatgccgtc
    27901 cgaacatatt tccagtattt tcagacggca tttcaagttt tcaaatccct caatgtccac
    27961 cgccgccgct gtggaacggc ggtttcgcca gccagacaat gggaatcagg acgataaaca
    28021 aaatgctgcc ggcgaggaat atttcgttcg atccgatgat gaagccctgt tgcgtaatgg
    28081 tattgttgat gatgcctaga gtttgaccgt cggaaatgcc ttgttgggac aaatgcgccg
    28141 ccgtttcgtg caatgttgcg gaatagggcg tgatgtgttc ggcaaaacgc gtgtggtgca
    28201 acgcttcgcg ccgttcccac agggtgctga cgacggatac gccgacaccg cccatcagca
    28261 cgcgcaggaa attcgacagg ctgccggcgg cggcaatctg tccgcccttc atgtgcgaca
    28321 gtgtgatggt ggtcagcggc aggaagaaca tggcaacgcc taccccctgc caaaactgcg
    28381 gccagatgac gttgccgata tccatgtcgg cataaaaatc agtgcgccaa taaaaagtga
    28441 aggcaaaggt caggaagctg gcggttacga gcaggcgcat atcgatttta ttgccgaacc
    28501 tgccgattaa cggagatagg aagacgggga ggatgccgac gggcgcggca gcaagccctg
    28561 cccacgcgga ggtatagccc aagttggtct gcaacactaa cggcagcagg gtcagcgtcc
    28621 ccatatacac catgaaaccc aacgaggtgg caattacgcc gacggtaaaa ttccgatctt
    28681 taaacagcga taaatcgaca atcggatatt tttctcccaa ttcccaaaca ataaaatacg
    28741 acaagcacac cagtgcgact acgcccaagg taatgatttc tccagaggcg aaccagtcaa
    28801 gttccttacc cctgtccagc atcatctgta acgcgccgat accgaccatc atcagcgtca
    28861 gcccgacata gtcggtcggc gttctgacgg ttgccgtttc gcggtgtttc aaatgtttcc
    28921 atgtaatcca tgccgatatg atcccgatgg ggatattaat gaagaaaatc caaccccaat
    28981 gccagtttcc ggaaatccaa ccgccgagta tcggcccgag gacaggggcg acaacgacgg
    29041 tcattgccca caatgccagt gccagcatcc gttttgcggg cggataggat gccattaaca
    29101 ggctttgcga caggggaatc aacggcccgg cgataaagcc ctgcaaaatg cggaaaacaa
    29161 ccagcgactg aaggttgggc gcgatgccgc acagccacga tgcgatgacg aaaccggcgg
    29221 cggcggcggt aaacaatttg acctcgccga tacgttttgc caaaaagccc gtcagcggca
    29281 cggaaacggc gtttgccacg gaaaaagaag tgatgaccca cgtcccctgc gtggttgccg
    29341 caccgaggtt gccggcgatg acggggacgg cgacgttggc gatggtcgta tctaaaacct
    29401 ccataaatac ggcaagcccc aaagacagcg taacccacgc caatgccgca cccttaagcg
    29461 gtggataatc cattgtgtcc tccgtttcag acggcatcag ggcgcggatt gcccgaggat
    29521 ttcatcgacc gtccgatcga cttccgacca gtcggtactt tccatttccg gcaatgccgc
    29581 acccggcgtt tttgaaacag gcgcgcctgc ggcggaagta tccactttaa ccgtcatcga
    29641 caaaccgata cgcaacggat gcctgtccac atcttcgcgg ttcagcacga tacggacggg
    29701 gacgcgctgc accactttaa tccagttgcc cgttgcgttt tgcgccggaa tcagcgaaaa
    29761 cgcgctgccc gtacctgccg aaaaccctgc caccctgccg cgataaacaa tttgtttgcc
    29821 gtacaaatcg gacaccagct cggcaggctg tccgattttc atatgccgca actgcgtctc
    29881 tttaaaattg gcatccaccc acacatccga cagcggcacc accgccatca gcggcgcgcc
    29941 tgccgccacc tgctgcccga cctgcaccga acgcttcgcc acctgaccgt ccgccggcgc
    30001 gcggacttgc gtccgccgaa ggttcagcca cgcatctttc aacctgccga ttgccgtctg
    30061 aacctccggc tgttcgcgca aagaaacatc tccgcccaaa gccgcgcgtg ccgaagattc
    30121 ttccgccaaa gccgccttaa ccgccgcctg cgcctgagac actgccgtac gggcgtgtgc
    30181 cagctcttcg gcggacaccg cacccgattc cgccaaagcg gaccggcggc gcaaatcatc
    30241 ctgcgcgcgc gccaaatccg cacggcgcaa ggcaacctgc gcccccgcct gcgaagtggc
    30301 ggcattttgc cggcggtttt ggcgcaccgc ctgaaccaac tcgttttttg cccgctcgta
    30361 agccagcaca tcattatcgt catccaacac cgccagcacg tcgccttttt tcacggcatc
    30421 cgtatcgtcg tgcaaaacct tccgcaccgt gccgcccttt tgcggcgtaa cctgaaccac
    30481 gcgtccggca acataagcgt cttccgtttc ctcttcgtgc tgccaccata aaaaaaacgc
    30541 cgatccggcg gctgcggcgg aaagcgcgaa cagcagcgtc aatgccgtca ggcggcgttt
    30601 gcgtttgact tgcgtgtttt gaagttttgt ttcgtctgtg tgcgtttcca taagattggg
    30661 ttccgaattt cctgatttca tataaacaat gttctttcaa taggaaatag attttatatc
    30721 cgcgcaataa cgcaatcaag tcagtatttt agatgcaata ttcaaaaaac aacgcttcag
    30781 gcggcaaaac tgaaaatata tttcgtttat atatgacatg gcaaacaccg ccgcccgcac
    30841 catgccgtct gaagaaaact acacagatac cgccgcttat attacaatcg ccgccccgtg
    30901 gttcgaaaac ctcccacact aaaaaactaa ggaaacccta tgtcccgcaa caacgaagag
    30961 ctgcaaggca tctctctttt gggtaatcaa aaaacccaat atccgaccgg ctacgcgcct
    31021 gaaattctcg aagcattcga caacaaacat cccgacaacg actatttcgt caaattcgtc
    31081 tgccccgaat tcaccagcct ctgcccgatg accgggcagc ccgacttcgc caccatcgtc
    31141 atccgctata tcccgcacat caaaatggtg gaaagcaaat ccctgaaact ctacctcttc
    31201 agcttccgca accacggcga ttttcatgaa gactgcgtca acatcatcat gaaagacctc
    31261 atcgccctga tggatccgaa atacatcgaa gtgttcggcg agttcacacc gcgcggcggc
    31321 atcgccgttc atccgtttgc caactacggc aaagcaggca cagagtttga agcattggca
    31381 cgcaaacgcc tgttcgagca cgacgcacaa taaaccatcc ttcccatatt tcagacggca
    31441 ttccttcgat acggtatgcc gtctgaacgc ctttccgaaa ggtttttatg tacgcattga
    31501 ccgccgcaca gcaacagaag gcactcttcc ggctggtgct tttccatatc ctcatcatcg
    31561 ccgccagcaa ctatctggtg cagttcccct tccggatttt cggcatccac accacttggg
    31621 gcgcgttttc ctttcccttc atcttcctcg ccaccgacct gaccgtccgc attttcggtt
    31681 cgcacttggc gcggcggatt atcttttggg tgatgttccc cgcccttttg ctttcctacg
    31741 tcttttccgt tttgttccac aacggcagtt ggacgggctt gggcgcgctg tcccaattca
    31801 acacctttgt cggacgcatc gcgctggcaa gttttgccgc ctacgcgctc ggacaaatcc
    31861 ttgatatttt cgtattcgac aaattacgcc gtctgaaagc gtggtggatt gccccggccg
    31921 catcaaccgt catcggcaat gcactggaca cgttagtatt ttttgccgtt gccttttacg
    31981 caagcagcga tgaatttatg gcggcaaact ggcagggcat cgcttttgtc gattacctgt
    32041 tcaaacttac cgtctgcggt ctgtttttcc tgcccgccta cggcgtgata ctgaatctgc
    32101 tgacgaaaaa actgacggcc ctgcaaacca aacaggcgca agaccgcccc gtgccctcgc
    32161 tgcaaaatcc gtaaaacagc tctgtaggga atcgggacgg ttgggttttc ttttatttgc
    32221 aaacactgcc ggaacggata aatccggatt cccacccact tcgtcattcc cgcgaaagcg
    32281 ggaatccggt gcgttggggt tcagtcattt ccgataaact cctgccgcat tgtatttcta
    32341 gattcccgct ttcgcgggaa tgacggcgga tgggtttccg tgccgtggtg tccggattcc
    32401 cgccggtacg ggaatgacgg cagcgcttcc ttggcttagt tttattttaa gccactataa
    32461 atgccgtctg aaaacctgtt tgcagatttt cagacggcat tgttttcgtc gccggttcaa
    32521 acttcaatcc gattgcgaca cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc
    32581 cgccttctgt ttctgcgcgg caaacgccgc cttgtccgcc tgcgtcaatt cgggtgtcgg
    32641 caatgcgacc gaaacaggat tgacgggctg cccgttgatg cgcgcctcgt aatgcaggtg
    32701 cggcccggtc gaacgccctg tcgaaccgac aaaaccgatg acctcgccgc cgcgcacatt
    32761 gccttgtgcc tgcgaaaacg cgctcaagtg cgcgtacagc gtttccacac cgttggcgtg
    32821 gcgtatcatc accgcgttgc cgtatccgcc cttccggcct ttaaaggtaa tcacgccgtc
    32881 ggcggaagcc ctgaccggcg ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg
    32941 ccatgtgtgc aggatggggt gcatacggta gccgaacggc gaagaaatgc gcgtatagac
    33001 cagcggctcg atgttgaagc cgcctttttc ctgcaacacc ctgccgtctt catcgtaata
    33061 attgccgccc cctccgcctt ccttgtccga acggtaatag aacgcctgat gggttgtgcc
    33121 gcccttgaca acttccgccg ccaaaatatc gcccgccgcc acctgctgcc cgtggaaata
    33181 caggctgtcg taaagcaggc gcacggcatc gccttccttc aaaccgtcaa ggctgaagcg
    33241 gccggcaaaa atcccgctta aggattcgcg gatttcgacg ggcacttccg cccgcgccag
    33301 cgaaccgcgc gccgacgttt tgaccacgac cgaacgcagt gtcggcaaaa ccttcatatc
    33361 cgcatcagaa gccgaccgcc gccatatgcc gccttttttt tccaaagcga ccagattgcg
    33421 ctcgccgtct tcgtcggtaa aaaactgcac ttcgcgcgca ctgccgtcgc cgccgaccaa
    33481 aacatgaacc gactggtcgg cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc
    33541 cgtgattcgg gcaatctcgt cccgcgccat acccgaacgc gccagcacgt ccgccagcga
    33601 gtcgcccggc tgcaccgcct cctgcaccca atatgccgtc tgaacgccgc tgccgcccca
    33661 agacagcggc ggcagttttt gttccacgcg ctgcggtctg acgcgctccg tcccctctgt
    33721 cgaagcaatg tatgccgccg acaccagaat aatcgaaacg gcaagcgcgc gcagcgcgta
    33781 tttccgatgt tttgccgaaa gtgggaagac agccatattg ttcctttttg acggcgtttg
    33841 cgccgaatcc tctataatat ccgcccgatc ctcatttgtc aggcatcaaa cgatgaaact
    33901 caccctcgcc ctcccctcgc tcaatttgga tgaagacgaa atccgaatac ctctatgcct
    33961 gcccgccttc aacaaaatcc tgcaatacgg ctcgccgcac cgccaaagct gcacggcttc
    34021 ggcattttac gcacgttatt tgtggtgcgg aagactggca caacgcccgg cgcaaagcct
    34081 gaatatgccg tctgaaaccg tcgcgctcgc cacgcccgtc tggcaaaaaa tggggctgca
    34141 tcaggcaaat gtgctgacgg cggaatattt ggatgtcggt acggatgaag cggaacggct
    34201 ctgccgcgat ctgtcggcgt tttacggcga cattccgtgg cgtttcgttc ccgtcctgcc
    34261 cgaattatgg ctggtttccc ttccccgcgc gtaccgctgg ggcgcgaagc ccgttttgga
    34321 cttgggcgga ctgttgggcg cggacgatca gccggacggg gaagacgcgt tggaatggct
    34381 gcgcgtccaa accgaaatcc aaatgtggct gaacgctcac cccgtcaacc acaaccgcaa
    34441 aaaacgcggg ctgcccgaat tgaacggctt atggctttgg gacagtctgc acggaagcgc
    34501 acaaggcggc acgcttttcg ccgatacggt ttggagccgt tttcacccga accgccgagc
    34561 attgcccgac agcttccgag cttatgcgga aacggcggca cacctgcccg atacgcacca
    34621 catcctgttt atggacgatt tgcgcctgac cgccctgaca ggggacaggg agcggtatgc
    34681 ggcaatattg cagcagtggg aagaacgctg gttcgcaccg ctttacgaag ccgtccgcac
    34741 cggcaaaatc aaacggctgg acatcgccac ggacggacaa cacggcggta cgctgacctt
    34801 caaacccaca gacaggcgga agttttggcg ctgcacgaaa acctttgacg gaatctggta
    34861 aacccgcccg ccgcaaccgc gacaatgccg tctgaagccc gaatcgggtt tcagacggca
    34921 ttttaccccg catcgccgtt atgccggaaa aaccggaata cgtttccgca cacgcggcgg
    34981 cacgcaaaac accgcccgaa agccgccgat gcccgccgtg acaccgccgc cggcagggtt
    35041 tccgccgaaa aggaacagca atcagaacgt tttgttaaac tcgacaaacg ccctgttttt
    35101 ctcgtattcg ttaaacacat cgttgctcca cgtttcgcgg tgcgacagcg tcaggcgcgg
    35161 cgtgatgcct ttgaaatgca atgcccggtg ccaaaggctc aaggatgtgt ccgattcttt
    35221 atccctgcgc ctttcccctt taaaactgct gaagaagccg ggtttttcat aatgccgttt
    35281 cgccacgccg aggcggaaca gcgaagacag gccgctgcca ccccattcct gcccccaggc
    35341 aaagcgcagg ccgtaacggt tgaaattgtc gccacggtcg gcggggttgc gctcgcggta
    35401 aaaatccaaa ccgcccgtcc aatattggcg cgcattccgg taaaacacca gcgaattgga
    35461 aatttgcaaa tgggtattgt cggaacgcgc ccggcgcgta ttcttcaaac gcccccactc
    35521 cgccgaagac agcgtttgcc atctcggggt ttgccaacgg ttgaaataaa ggcgtgcgcc
    35581 gttggcgtaa gaataagcgt cgttgccgta ggtgcggcgt tcgtggaaca ctgccagccc
    35641 gacatcttta cgccggtcgg caaaaccgat gccgccggaa acacctgccg tcatatcgtt
    35701 gaatttctta ttccccggat aaaccctgcc ggacacgtcg ccgcccgccg tcgtgtacca
    35761 gccgtttttc agcgaccatt ttttctccgc gccgaaccgg taattgaccg ccgtgccgtc
    35821 cacctgtttc gggaaagtcc aattgccgta ctgctgctgt ttcggggctt ggttgatatt
    35881 gtgttcgcgg gtaacgctga gaccgccgtt taccttccac gcatcgcgtt cgcgcaatgc
    35941 cttgcggtac agctcgacct gctccataag ctgcggcggc agatcttctg ttttcaggcg
    36001 gtcgaactgg tctgccgccg cctcgttctg cctgtcttca aacaatgccg ccgccaaacg
    36061 catacggacg gcgggcgcgt cgggttgggc ggcaatcaat tcccggtaat gggaaaccgc
    36121 ctccttcacc ctgccctctg cctgcgccaa aatcccttgt gcataaagtg ccaacatctt
    36181 atcctgccgc gcctgttgta ggtaaatcgg caaaatcacg cggataccgg caatattgtt
    36241 tgagaccact gcggaataca tggcacgcga caacaattcg ggatttttca gcagggtttc
    36301 gccgtcaacc tgcaaaactt ttcctttttc ccgcacctgc cccggtacct tctccctgtc
    36361 gatcggtttg acctccgctt catgaagcct gaactcggga cggcttctca aatccggttc
    36421 gcgcggtgtt tcttcggcgt atgccgcgct tgccaataaa ggcagcagca tccatctgtt
    36481 tcgtgcaggc ataaatgtct tcccacaaaa ataaaaataa aaaataacca ttatcaaccg
    36541 gcggattgta tcagtttttt atgccgctgt tccaaaccct gccgcccgat gccgcgacaa
    36601 tgccgtctga agccagaatc gggtttcaga cggcggcaaa aaaccggcgg ttttccgccg
    36661 gtttcatcat tatgccccgc ccttctgggc ggcagggttc ggatcggttt gcggggcggc
    36721 gcacggggcc gtgcaggtgt gccgcctgtg tgtcaaaaca tttcccggcg catcaaaaca
    36781 accatttgac cgacaatgcg gcttctttgt cgccgtccgt ccttttgccg tatccgatgc
    36841 gtgcggacat atggcctttc caaccggctt caatgccaaa ccgcccttcg agcgccgtcc
    36901 tgcctgccag cgtctgtttt tcgccgtcca tttccatgcc gaaagatttt gacctgtgca
    36961 aaacattaaa agcggcaaaa ggctgaagat tgacaccgtt acgcaaagca aaacgggttt
    37021 ttgcccgaat gccggcgcgg atttgccact gaccgctgcc gagcagcccg accgccgccc
    37081 ctcgctgtcg gtaaagccgc cgtttacgcc caagtaggta aactgcgcct gcggttgcag
    37141 gtaaaactgc atattactgc cttatatatt gcgtccctaa gaaggggcga ttaacaaaaa
    37201 ttaacgcccc ttcttaggga cgcaatatat aaggagtgag tacctgttct tctattaaag
    37261 aattaatctt ttgaggcatt ttctgttgaa gtttattaaa cacatcttca gaaattccaa
    37321 actgctttat tatctcttcc ctaacccgtt ttttattttc ttcccaagcc tcgggtcttg
    37381 ttttgtataa atcctgtaat tgctttttaa ctgcctgctt taaatcagtt cggtaattat
    37441 ctgtaatatt gatttcgtca tacaaggctt cagatgattt ttgatcggtt ttaccgctgg
    37501 agaaataatg gaaaacaccg tttgccggct tgtctgaaaa tgaaaaatcg gcagtatcgc
    37561 caacatttgt aggacgagta tccgaaccgg gctgataact gaaagcaccg aagcaatcaa
    37621 aagacacact gcacgggccg cgccaaacgt gggaatttct gtcttccgcc gcttctaaaa
    37681 taaatttgtt tttaatctcg gcttctttga cgggatcgta cgcatccgaa ccccaagcat
    37741 atggcgtgca aaacagcgat aaaacagata atgttaatag attaacccga ccgcttgctt
    37801 gctttcagtg tcatgagaaa ttttcctttg tcaagtgtta aagttataat gattatatac
    37861 tatatcacac tacatcgcaa tgaaaaatcg ggaaaaacaa aaaacccctc cgccgtcatt
    37921 cccgcgaaag cgggaatcta gaattttaat gcggcaagaa tttatcgggg atggccgggg
    37981 tttaaaggtc tgggttcccg cctgcgcggg aatgacggtt tagaaatcac ccgaaacgag
    38041 aaaaagaacc gaaaccggac aagtcggatt cccgcgcggg cgggaatgac gataagcgta
    38101 atttcaatcc gccatattgt cttttcactt gcctgaaaac agatttcagc cgcctatccc
    38161 tgcatccggc ggctgatgta gtcggcaatc aaatcccgct tttcccaaaa aaacttctgc
    38221 attttttgtt cggaatacag tttgcggacc agagtgggca gaccgctgtg gacgatggcg
    38281 gcgatttctt tgttgccttc ttcttcctgc attgccgtct gaaaatccaa acccctgcct
    38341 ttcagatatg ctgcaaggcg gttgcggatg tgtgaattga gttcgatgga tttgagcatt
    38401 ttgcctccgt tttgcggtaa ggatttattc gatttgttta tccgcctgat tttatcacgt
    38461 ttcccaagcg gtttgcctgt acaataccgc ttttcggaag ggtgtgggcg atgatgtttt
    38521 cttggttcaa gctgtttcac ttgttttttg tcatttcgtg gtttgcaggg ctgttttacc
    38581 tgccgaggat tttcgtcaat atggcgatga ttgatgcgcc gcgcggcaat cccgagtatg
    38641 tgcgcctgtc ggggatggcg gtgcggttgt accgttttat gtcgcctttg ggtttcggcg
    38701 cggtcgtgtt cggcgcggcg ataccgtttg ccgccggccg gtggggcagc ggctgggttc
    38761 acgtcaaact gtgtttgggc ttgatgctct tggcttatca gttgtattgc ggcgtgctgc
    38821 tgcgccgttt tcaggattac agcaatgctt tttcacaccg ctggtaccgc gtgttcaacg
    38881 aaatccccgt gctgctgatg gttgccgcgc tgtatctggt cgtgttcaaa ccgttttgac
    38941 ggagcatctt atgccgatag aaatcgaacg ccgttttttg attgaaaacg acaaatggcg
    39001 gcaatacgcc gatgagccgc tgctgctgaa acagggctac ctgtctgttg aaaaagaacg
    39061 caccatccgc atccgcattg ccggaaaacg ggcgtggctg acgctgaaag gctatatttc
    39121 ggaaatcagc cgcagcgagt tcgaatacga aatcccgctt gccgatgcgg aaaagatgat
    39181 ggaaacgatg tgcccgttta agatggaaaa acggcgttat ccggtcagat ggggcggcag
    39241 cctgtttgaa gtcgatgttt tccttggcga aaactcgcct ttggtcgtcg cggaaatcga
    39301 gttgcccgcc gaaaatgccg attttgaccg tccggactgg ctggggcgcg aaatcacttc
    39361 agacggtatg tttaccaatg cctatttgag caaacatccg ttctcaagct ggaaaaatgc
    39421 cgtctgaagc ggcttcagac ggcatctgcc ttgcggttat cccttgaggt aatgcacgaa
    39481 tgtatatgca acgcctttgc tgctgacacg gcgttccgtc cgctctgctt ctttccaatg
    39541 cgtccggtct atttctggga aaaatgcatc tccttccaca gacaaatcca cttcggttat
    39601 ccgcaaatcg gtcgctaatg gcatcgcttg tccgtatatc tgcgcgccgc ccataatgac
    39661 ggcttcttcc gcgccggcgc acaatgccaa tgccacctcc aaacttgcca ccgtttccgc
    39721 gcctgccgcg caataatccg cctgccggct gatgacgatg ttcctccgtc cgggcagggg
    39781 tttgacgggc agggattccc acgttttccg ccccataatg acgggtttgc ccaaggtata
    39841 gactttgaaa aatgcgaaat cttcggggat gtgccaaggc atagcattgc ccgccccgat
    39901 gcacaggttt tccgcacacg ccgcaattat ggttattttg agcatatttt ctccacaaaa
    39961 atgccgtctg aacgcttcag acggcatttt atgacattct gcgtcaaaaa tctcaacctg
    40021 cgcgcgccat acgttttgtg ttcgcacccg ccaacaatgc caacaattct tcagtcgcgc
    40081 cccaaccgat acacgcatcg gtaatgctct tgccgtacac ttccggcttg tcctgcctgc
    40141 cttcgaccaa atggctttcc accatcacgc ccatgatatt gccgccgtcc tgttccaatt
    40201 ggtcggcaat gtcttgtgcc acttccatct gccgggtgta atccttgcgg ctgttggcgt
    40261 ggctgcaatc gatcatcagc ttgtcggtta cccctgccgc acgcagttgt tccgccgcct
    40321 cgctgacgtg tcccgcatca taattcggct cttttccgcc gcgcaaaatg acatgacagt
    40381 cgggattgcc gccggtatgg acaatggcgg aatgcccggc cttggttaca gacaagaaat
    40441 gatgcgaatg gctcgccgca ccgattgcgt cgatcgcaat cttcaaattg ccgtccgtac
    40501 cgtttttaaa gccgacgggg caggacagac cgcttgccaa ttcgcggtgg acttggcttt
    40561 cggtcgtccg cgcgccgatt gccccccaag aaatcaggtc ggcataatac tggggtgtaa
    40621 tcatatccaa aaattcggta gaggcaggca tgcccatatt attcagggac aacaacaggc
    40681 tgcgcgcctg acgcaaaccg aaattgatgt cgaacgtacc gtccaaatgc gggtcgttaa
    40741 tcaaaccttt ccaacccacc gtcgttctcg gcttctcgaa ataaacgcgc atcacgatca
    40801 aaagctcgtt ttcataccgc ttgcggagtt tcaacagacg ttccgcatat tccaacgccg
    40861 ctttcggatc gtgaatcgaa cacggcccga taatgaccaa cagccgcctg tccctgccgt
    40921 gaaccaaatc ggaaatttcc tgacgggtgc ggtgaaccaa gcccgaagcc tctttggaaa
    40981 tcggcagctc gtaaagatgg gcaatcggcg gcaacaactc tttaacttct ttaatcttaa
    41041 tatcgtctgt ggggtaatgg tgtgtcatct tgcatctttc ctgatgggtt tttatacgct
    41101 gcttaggtta catcgtttac cttgcacaaa caagtgcaac atattactac aaagctattt
    41161 tatttcaaaa taactgatat atcttttttc atagatttta aatttcatat ttttaatatt
    41221 ttaggaattt tatttcaaag aaaaagaaac ag
//
LOCUS       DAXFHF010000009        40993 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXFHF010000009 DAXFHF010000000
VERSION     DAXFHF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40993
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.009"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            210..1589
                     /gene="radA"
                     /locus_tag="ACFIRM_000418"
     CDS             210..1589
                     /gene="radA"
                     /locus_tag="ACFIRM_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC3028120.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
     gene            complement(1864..2343)
                     /locus_tag="ACFIRM_000419"
     CDS             complement(1864..2343)
                     /locus_tag="ACFIRM_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC3028121.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(2468..2827)
                     /locus_tag="ACFIRM_000420"
     CDS             complement(2468..2827)
                     /locus_tag="ACFIRM_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3028122.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(2866..6468)
                     /gene="recB"
                     /locus_tag="ACFIRM_000421"
     CDS             complement(2866..6468)
                     /gene="recB"
                     /locus_tag="ACFIRM_000421"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC3028123.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPCLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIAHALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETIDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            complement(6545..7453)
                     /locus_tag="ACFIRM_000422"
     CDS             complement(6545..7453)
                     /locus_tag="ACFIRM_000422"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC3028124.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGREIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            7738..8565
                     /locus_tag="ACFIRM_000423"
     CDS             7738..8565
                     /locus_tag="ACFIRM_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3028125.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADKKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            8555..9271
                     /locus_tag="ACFIRM_000424"
     CDS             8555..9271
                     /locus_tag="ACFIRM_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3028126.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLATVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            9281..10039
                     /locus_tag="ACFIRM_000425"
     CDS             9281..10039
                     /locus_tag="ACFIRM_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3028127.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALA
                     IQPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMD
                     GGVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(10095..11477)
                     /locus_tag="ACFIRM_000426"
     CDS             complement(10095..11477)
                     /locus_tag="ACFIRM_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC3028128.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIAIGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(11645..12154)
                     /locus_tag="ACFIRM_000427"
     CDS             complement(11645..12154)
                     /locus_tag="ACFIRM_000427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3028129.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            complement(12274..13689)
                     /locus_tag="ACFIRM_000428"
     CDS             complement(12274..13689)
                     /locus_tag="ACFIRM_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC3028130.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRMIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            complement(13918..14280)
                     /locus_tag="ACFIRM_000429"
     CDS             complement(13918..14280)
                     /locus_tag="ACFIRM_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC3028131.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            complement(14277..14483)
                     /locus_tag="ACFIRM_000430"
     CDS             complement(14277..14483)
                     /locus_tag="ACFIRM_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC3028132.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            complement(14544..15122)
                     /gene="pth"
                     /locus_tag="ACFIRM_000431"
     CDS             complement(14544..15122)
                     /gene="pth"
                     /locus_tag="ACFIRM_000431"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC3028133.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDILAGKWEEATRFLHSK"
     gene            complement(15175..15453)
                     /locus_tag="ACFIRM_000432"
     CDS             complement(15175..15453)
                     /locus_tag="ACFIRM_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC3028134.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            complement(15446..15877)
                     /locus_tag="ACFIRM_000433"
     CDS             complement(15446..15877)
                     /locus_tag="ACFIRM_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC3028135.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            complement(16099..18066)
                     /gene="ftsH"
                     /locus_tag="ACFIRM_000434"
     CDS             complement(16099..18066)
                     /gene="ftsH"
                     /locus_tag="ACFIRM_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC3028136.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIQTLLNKNVRVKVTPEEK
                     PSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            complement(18130..18750)
                     /locus_tag="ACFIRM_000435"
     CDS             complement(18130..18750)
                     /locus_tag="ACFIRM_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC3028137.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            18860..19144
                     /gene="yhbY"
                     /locus_tag="ACFIRM_000436"
     CDS             18860..19144
                     /gene="yhbY"
                     /locus_tag="ACFIRM_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC3028138.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            19328..20329
                     /gene="hemB"
                     /locus_tag="ACFIRM_000437"
     CDS             19328..20329
                     /gene="hemB"
                     /locus_tag="ACFIRM_000437"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC3028139.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(20393..21550)
                     /locus_tag="ACFIRM_000438"
     CDS             complement(20393..21550)
                     /locus_tag="ACFIRM_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC3028140.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            21754..22527
                     /gene="folE2"
                     /locus_tag="ACFIRM_000439"
     CDS             21754..22527
                     /gene="folE2"
                     /locus_tag="ACFIRM_000439"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC3028141.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            22724..23389
                     /locus_tag="ACFIRM_000440"
     CDS             22724..23389
                     /locus_tag="ACFIRM_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3028142.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWRFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(23444..24202)
                     /locus_tag="ACFIRM_000441"
     CDS             complement(23444..24202)
                     /locus_tag="ACFIRM_000441"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3028143.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(24227..25117)
                     /locus_tag="ACFIRM_000442"
     CDS             complement(24227..25117)
                     /locus_tag="ACFIRM_000442"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC3028144.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            complement(25137..25703)
                     /locus_tag="ACFIRM_000443"
     CDS             complement(25137..25703)
                     /locus_tag="ACFIRM_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC3028145.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            complement(25753..26529)
                     /locus_tag="ACFIRM_000444"
     CDS             complement(25753..26529)
                     /locus_tag="ACFIRM_000444"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3028146.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDAFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECDETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            complement(26584..27234)
                     /locus_tag="ACFIRM_000445"
     CDS             complement(26584..27234)
                     /locus_tag="ACFIRM_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC3028147.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKIGNGNP"
     gene            complement(27246..28286)
                     /gene="murB"
                     /locus_tag="ACFIRM_000446"
     CDS             complement(27246..28286)
                     /gene="murB"
                     /locus_tag="ACFIRM_000446"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC3028148.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPLYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPTSFSL"
     gene            complement(28330..29709)
                     /gene="norM"
                     /locus_tag="ACFIRM_000447"
     CDS             complement(28330..29709)
                     /gene="norM"
                     /locus_tag="ACFIRM_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC3028149.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHEAV"
     gene            30047..31198
                     /locus_tag="ACFIRM_000448"
     CDS             30047..31198
                     /locus_tag="ACFIRM_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC3028150.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            31303..32601
                     /locus_tag="ACFIRM_000449"
     CDS             31303..32601
                     /locus_tag="ACFIRM_000449"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC3028151.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(32837..33676)
                     /gene="htpX"
                     /locus_tag="ACFIRM_000450"
     CDS             complement(32837..33676)
                     /gene="htpX"
                     /locus_tag="ACFIRM_000450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC3028152.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            33891..34538
                     /gene="adk"
                     /locus_tag="ACFIRM_000451"
     CDS             33891..34538
                     /gene="adk"
                     /locus_tag="ACFIRM_000451"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC3028153.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            34907..35647
                     /gene="pyrF"
                     /locus_tag="ACFIRM_000452"
     CDS             34907..35647
                     /gene="pyrF"
                     /locus_tag="ACFIRM_000452"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC3028154.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            35703..36665
                     /gene="hldA"
                     /locus_tag="ACFIRM_000453"
     CDS             35703..36665
                     /gene="hldA"
                     /locus_tag="ACFIRM_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC3028155.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDVIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            36729..37733
                     /gene="rfaD"
                     /locus_tag="ACFIRM_000454"
     CDS             36729..37733
                     /gene="rfaD"
                     /locus_tag="ACFIRM_000454"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC3028156.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            37808..39352
                     /locus_tag="ACFIRM_000455"
     CDS             37808..39352
                     /locus_tag="ACFIRM_000455"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC3028157.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIET"
     gene            39468..40319
                     /gene="dinD"
                     /locus_tag="ACFIRM_000456"
     CDS             39468..40319
                     /gene="dinD"
                     /locus_tag="ACFIRM_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC3028158.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            40486..>40993
                     /locus_tag="ACFIRM_000457"
     CDS             40486..>40993
                     /locus_tag="ACFIRM_000457"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3028159.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFT"
BASE COUNT         9220 a        10499 c        11796 g         9478 t
ORIGIN      
        1 tctaccatta tatttaatgg gaaagtcatt gtttgggatt cctgttttgt attttccgtc
       61 attatgccac aaaataacag aaagccaccc ttgtttgtat cgtgatatgg tcttgtaatc
      121 aaaattaaca aactaataat aaaaaatttt ttagaaaaga agtttcagac gattttttta
      181 cacttcaaaa atcccatagg gaaaacatca tggcaaaaac gcttaaaacc ctttaccaat
      241 gcaccgaatg cggcggcact tccccgaaat ggcagggcaa atgtccgcat tgcggcgagt
      301 ggaacacgct tcaggaaagc tttgccgcgc ccgagccgaa aaacgcccga ttccaatctt
      361 gggcggcgga cacttcgacc gtccaatccc tctccgccgt taccgctgcc gaagtgccgc
      421 gcaatccgac cggtatgggc gaactcgacc gcgtattggg cggcggtttg gtcgatggcg
      481 cggtcatcct gctcggcggc gatcccggca tcggcaaatc cacgctgttg ttgcaaacca
      541 tcgccaaaat ggcgcaaagc cgcaaagtgc tgtacgtttc cggcgaagaa tccgcccaac
      601 aggtcgccct gcgctcgcaa cgtttggaac tgcacgccga aggcgtaaac ctgcttgccg
      661 aaatccgcat ggaagcgatt caggcggcct tgaaacagca tcagcccgaa gtcgtcgtta
      721 tcgattccat ccaaaccatg tattccgacc aaatcacgtc cgcccccggt tccgtgtcgc
      781 aggtgcgcga atgtgccgcc caactgacgc gtatggcgaa acaaatgggt atcgccatga
      841 tactggtcgg acacgttacc aaagacggag cgattgccgg cccgcgcgtg ctggaacata
      901 tggtcgatac cgtgctgtat ttcgagggcg accaacattc caactaccgc atgatacgcg
      961 ccatcaaaaa ccgtttcggc gcggcaaacg aattgggcgt gttcgcgatg accgaaaacg
     1021 gtttgaaagg cgtatccaac ccgtccgcca tcttcctcgc cagctaccgc gacgacacgc
     1081 ccggttcgtg cgttttggtt acacaggaag gcagccgccc gcttttggtc gaaattcagg
     1141 cattggtcga tgacgcgcac ggcttcacac ccaaacgcct cagcgtcgga ttggaacaaa
     1201 accgccttgc gatgctgctt gccgtgttaa accggcacgg cggcatcgcc tgtttcgatc
     1261 aggatgtttt tttaaacgcc gtcggcggcg tgaaaatcgg cgaaccggcg gcggatttgg
     1321 cggtcatcct cgcgatgctt tccagcttcc gcaaccgccc tatgcctgaa aaaaccgtgg
     1381 tcttcggtga aatcggccta agcggcgaag tccgccccgt cgcacgcgga caagagcggc
     1441 tcaaagaagc ggaaaagctc ggcttcaaac gcgccatcgt ccccaaagcc aatatgccgc
     1501 gcaatgccaa agagtttccg aacctgaaaa tccacggcgt ttcaagtttg caggaagcca
     1561 tcgatatttg ccgcgacagc agggaataaa cggaaatgcc gtctgaaatc aggtttcaga
     1621 cggcatttgg tttgtggcgg attgaaacaa gaaggcatac cggcgacaga taagatttgc
     1681 ggcaaagttg cctgtgatat ggcaaaacgc atacgcccgt catccctgcg aaggcgggaa
     1741 tccggaatcg tccgtttcgg caatgattgg aaatcacggt aacccaaccg cttggattcc
     1801 cgacttcggg ggaatgaggg gcgtgtgcat ttgatttcca tccgccatat gtcggcgacg
     1861 ggcttattcg cctacggttt tttgtatcag tttttcggcg tttgccaaag tgtttgccac
     1921 ttcgtcgaaa ctgatgcggc tgccgacgat gagggcgcgc gtatcggtat aggcggtgcg
     1981 tactttgccg tccgtttcgg taacgaggac gcgcaggggc agttgcaggg cgaaggcggg
     2041 gtctttgacc atcagcggcg taccggcctt gggcgtgccg aagacgatga cttttgccgg
     2101 ctgcatggtc aggccgtttc ggcgtgccgc ttcctgatgg tcgatgacgg caaaaatgtc
     2161 catccctttg ctttttatgg cagtttcaag gcggctgacg gtttcgtcaa aactgtattt
     2221 cgaggtgagg gtgtgcgtgg tcatagcggt ttcgttttgg gtgggcggtt tgccggcagg
     2281 gtgtgccgaa gcggttgaaa tgcagagtgc ggatgcggcg atcaggggga gtatgtgttt
     2341 catcgtattt ccttatcggt ttgtttatcg ggcttcggac ggggcggcac gcgccgcgcc
     2401 tgatgccgtg ccgcgtgccg gaacgccgta tgccgtctga aagcctgtcc tttcagacgg
     2461 cattgcgtca tttcatccct tttttgagca ggtcttcata accgccgtga ttggcaacat
     2521 ttgtataacc tgcttttttc agctcttgaa gggcggcttc ggcacgccgt ccgctgcggc
     2581 agtagaggtt gaccggcgtg tctttgtcgg gcgcggcttc gtatatgcgg cggacgattt
     2641 ggtcgacggg gatgttgacc gcgttgtgca aatgaccttc gctaaattcc tgttcggaac
     2701 ggacatcgat ccaaacggct gaatgttgcg cggtttgggc ggcggatacg ggtttttgcg
     2761 gggctgcctg cgtggcaaag gcggctgagg caatgagtgc ggcggtaatc aattgtttga
     2821 tattcatagg gttctcctgc ggttgtcgtc ttaagggacg ggaagttatt ttatctgttc
     2881 caaagcggcg gcatctatgt cccaacgcca aacgccgccg cctttgccat ccaatccgcg
     2941 caaaaacagg tagcggacgg aaacggcggc gggcggttgt ccgcgcagtt tgaagtagcg
     3001 cgcggcggca acggcgtaaa tcagtgcctg aaggtaatag tgctggtgcg cgacggcttc
     3061 gtccatcgcc tgccgcgtgt aggcggatag gtggttggat ttgtagtcga tgatgcagat
     3121 attgccgtcg gggtcttggc agaccatatc gataaagccg tttaaaaagc cgttgacggt
     3181 gtggaagtcg atcgtctcgg cggcggcacg gcagacttcg ggcagcctga tgtcgtcgcg
     3241 ggcaagccag tcgcgcagcc gtttgaggcc gaagtcttcg gtgtggaggg taaagcccat
     3301 ttcgggacgg cggcacccgg gcgggatgtc ggacaggccg tatgccccag tcagcggcgt
     3361 tttgcggcag gcttccgcca tttcggcaac ggcgggcagc catatttctt caaaaccgta
     3421 ttttttcagc ttgtcggcaa tgagggtttc ctgttcggcg gctgcttgtc cgaatttgaa
     3481 atcttcaaga atttcgtgca ggcacagccc cgcctgcgtg cctttcggaa agtcgtgtat
     3541 cgatatgccg tctgaagccg tcggcgtttc agacggcatc gccggcaccg aggtttcggc
     3601 ggcatccaag gacgggcagg catcttcttc gccgccgtcg ggcgtttggg tttggcggct
     3661 taaggcggtg aagctggtgt ggcggacaaa tcggaatccg cgttcgggaa tgctgtttgc
     3721 ggcaaattcg gcggcttgat cggcgttgcc gcgataggcg gcagggggcg gcgcatcttc
     3781 tgtgaaggcg aaatccgtgc cggccggggc attgtccgcc acgcgccgcc agttgcgttt
     3841 gagcatcgtt atgccgtctt tttcacacgc ataggtacgg cggacggttt cgcggctgtc
     3901 ttgcggcaag ccttcgatca ggtaggcaag ggggttgtcg gcggtatcgg aagaataggc
     3961 ggcgtagatg ttgagctgtt cctcggcacg cgtcagcgcg acataaagca ggcgcaggcg
     4021 ttccgccatt tcttcgtcgg cgtattgctt ctgttcgctt tccgacagtt gcgcctttgc
     4081 caaaagttcg gcccggtttg cgccttggtg gaggatttgc cagtcggacg gtccggtatc
     4141 ttgcgcgtcc cacgcaaacg ggcagtacac cagcggatac tgcaaacctt tcgaggcgtg
     4201 catggtaacg attttgacca aatcttcgtc gctttccaaa cggatggcgc ggttgtcgcc
     4261 gccgttgttg ccggccaggc tgatttgatc gcgcagccat ttgtgcagtg cggcggggtt
     4321 gcggttttgc gcgtcttcgg cggcaagcag ttcgagcagt tggaaataat tggtcaggct
     4381 gcgcccgttg ttccggctta agaggcgcgt ttcgatgccg tgtgtttggg aaaactgctg
     4441 catagcggcg aaaatgccgt atttttgcca tatgtcgagc gcggttcggg cagattccgc
     4501 ccaatgcaaa atctcgcttt cgttttggtt gaagtcgtgc aattgctgcg cgtcataacc
     4561 gaatatgccg cttgtcagga caaagcgcag cgttccggcg cggcgcggtt cgagccagaa
     4621 gccgatgagc gcggacaggg cggcggcttc gggcgaggcg aacacggatt cgcgcgaaag
     4681 caggacgctt tgcacctgcc gttttttcag tgcggcggaa atcatcaccg cctcgttgtg
     4741 cgtgcgtacc agaacggcaa tatcgcccga ctgcaacggg cggcctttga aattcagacg
     4801 gcctccggag gcttcgttga gcgcgtgggc gatttcgtcg gcgcaatagt cggcggcacg
     4861 gcggcgcaaa acgtctttgt tggctttttc attgtcgttt tcgtgcagcc aacggagctg
     4921 tacggcagga cgtttgggcg acaacctgct ttcggcacgc gccgcaccga cttccgaata
     4981 gccgatgttt tccaaaacga acgggcgttc tttgaggcgg aacagcgcgc cgatgctgcc
     5041 gataagcgcg gcgtggctgc ggtagttggt ggcgagcgtg tagcggtgcc gcgcgtcttc
     5101 cgccgcctga aggtaggcgt aaatgtccgc accgcgaaag ctgtaaatcg cctgtttggg
     5161 gtcgccgacg aggaacagcg ggcggtttcg ggcgatgaaa atcttttgga agatttcgta
     5221 ttgcagcggg tcggtgtctt ggaactcgtc gatcagcgcg atttcccagt tttcggcaac
     5281 ggcgcgggcg ggggtttcgg cgtgcggatt gtcggtcagc gcggtgtgga catcgagcag
     5341 caggtcgtcg aaaccgcgtt cgcggcgcga ttttttcatt tcggcaaggc tgctgttgag
     5401 atattcgatt aaatccagtt gcagccggat cattgttgct tcttccgctt cttcgagtgc
     5461 gttcaaatcg cgcccgaagt ctgccagttt ctgcaattcg gcaaatactg ccgcatcggg
     5521 cgttttgcct tttttcagtc cggcttcgag tttgtcggat gagagtttca agagccgttc
     5581 gtgcgtgtct ttgtccagac agggcagttg tccggcggcg gatttttgtg ccagttcttt
     5641 aaaaaggttg ccgaagctgt ttttgcggta actgttgccg ttgaggtcgg gatggatgcg
     5701 ccaaaagccg gcttccagct cgggcagcag gcggcagacg gtttgccatg aggtttcggc
     5761 gtcgcgctgc gcctgtttca aatccgcctg cggacggcgg aaattcaggt acgggcggga
     5821 caggtaggcg cggatttggg caaggacggt ttgcggcaca gctttgcgtt taaacgccaa
     5881 tgcggcaagc accgggtcgc cgctgacgcg ttcccgccaa aaatcttgag ccgggacaag
     5941 caggcggtcg ccgtcttctt cggtcagttc gacatcgaac ggtgcttggc acaggaaggc
     6001 gtagtcgcgc aggatgcgct ggcagaagcc gtggatggtg tagatggcgg cgttgtcgaa
     6061 ttgcccgatg gcggccttga ggcggacaat cagacgcgtc cggctttctt tttgcaaagc
     6121 ctgttccaag agtgcgggca ggaaggtgtc gccttcgtgg tgttcggcgc agtaggcggc
     6181 aatgccgtct gaaagcgtgt cgtctccaag ttcggcaatt tctttgcttt ccaaaacctg
     6241 caacacatcg tccaaacgcg cgcgcaggcg tgttttcagc tcggcagtgg cggctttggt
     6301 gaaggtaacg accaatacgc gctcgacgct tttttgttcc aatacgatca ggcgcgtaaa
     6361 cagggcggca atgccgtagg ttttgccggt gccggcagag gcctcaatca ggttggtgcc
     6421 ggaaatgggg gcggtcagcg ggtcgaatgc tcggatgggt gcggacatag cgtgtatgaa
     6481 aacggttgga cggtaaaacg ggaaaatgcc gtctgaaaaa gggtttcaga cggcatcgtc
     6541 cggcttagag gttttgcagg cgttcgacag acggcgcgta gtagtatccg cccgaaacgg
     6601 cggcggagag gtgtcggagc agcaggtcgg ttttgccgtc cgtgtcgccg aacatactca
     6661 ataattgcgc ttcgatatta tgcagcgtgc ggcagtatgc ggtaaacatc aaaccgtgtt
     6721 cgccgctgat tttgccgaag ggcaggctgc ggcggacgat tttcaggccg actccgtttt
     6781 ctttcaggtt gacgcggccg aggtgcgaat cgggcaggcg gacatcgcgg ctgaattcgt
     6841 cgtcggtttc cttgctgcgt ccgaccgagg cctcctgttc ggcgacgggg acggcatccc
     6901 attttttcaa atcgtgcagg tatttttgca gcaggacata gctgccgcct gcatcgggca
     6961 gcccttcggg gatgatggcg acttcgcgga ttgtttcatc gccttgcggg ttttccgtgc
     7021 cgtcgacgaa accgtccagc ccgcgatcct gatacaggcg caaaccgtgt tcttcggacg
     7081 cgacgcatat gctgtcgccg aacgcgccca aaacggattg ggcaagcgcg taggcggcgt
     7141 tttggcggaa ggattggatg tggatggaca tatcgtgctg cgtggacggc gcaagcccgt
     7201 tgcccatttc ggggaagggt ttgatttcac gcccctcgtc cgtgtgtccg aatgttgccc
     7261 aagctttgct gccgaaggcg atggtcagac ccaaaatatc gtccggaaag cgggctttca
     7321 aggcggataa cgcgtcgagc gaagcgcggc aggcggcttt aatatcgttg aggcggttgg
     7381 cggcgaagtc ggcttcgata aagatgccgg cttgggcgtg gtcgggaatg atggcggatt
     7441 ggggcgtgtt catgagatgt tcctttttgg tgtcatctgt ttcgggtgga ttatagcacc
     7501 gaatcggcag gcggattttt gccggaacgg cgtgcgtgaa tccgccgttt gcatacctga
     7561 tgccgctttt cggtttcgtg ccgcccgccg cctttcccgc cccctttatt tccgcttccg
     7621 gcggcttcgg catatctttc ccattccgat ttggaataac catataaaaa aagtattctt
     7681 tgtgtttgcc gcaatttcac ttagaatgcc gcacttgcac actttttaca ggagggaatg
     7741 atgttgaaaa aattcgtact cggcggcatt gccgcattgg ttttggcggc ctgcggcggt
     7801 tcggaaggcg gcagcggagc atcttccgcg cctgcacaat cggcaatttc cggttcttta
     7861 atcgagcgca tcaacaataa aggcacggtt accgtcggca cggaaggcac ttacgcaccg
     7921 tttacctacc acgacaaaga cggcaaactg accggttacg acgtggaagt aacccgcgcc
     7981 gtggcggaaa aactgggcgt aaaagtcgag tttaaagaaa cgcaatggga ttcgatgatg
     8041 gcgggtttga aagccggacg tttcgacgtg gtggcaaacc aagtcggcct gaccagcccc
     8101 gaacgccagg cgacatttga caaatccgaa ccttacagct ggagcggtgc ggttttggtt
     8161 gcgcgtaacg acagcaacat taaatccata gccgacatca aaggcgtgaa aaccgcgcaa
     8221 tccctgacca gcaactacgg cgaaaaagcc aaagccgcag gtgcgcaact cgtgccggtg
     8281 gacggtttgg cgcaatcgct gaccctgatt gaacaaaaac gcgccgatgc gacgttgaac
     8341 gatgaattgg cggttttgga ctatctgaag aaaaacccga atgcgggggt gaaaatcgtg
     8401 tggtccgcgc ctgccgataa aaaagtcggt tccggtctga ttgtcaacaa gggcaatgac
     8461 gaggccgtgg caaaattcag cacggcaatc aacgagctga aagccgacgg cacgttgaaa
     8521 aaactgggcg aacaattctt cggaaaagac atcagtgttc aataatttcc ttgcctctct
     8581 gccgtttatg acggaaacac gcgctgatat gctcatcagc gcgttttggc ccatggttaa
     8641 agccggcttt acagtgtctt tgcctttggc gatcgcttct ttcgttatcg gcatgattat
     8701 tgccgtagcc gttgctttgg taagaatcat gccttccggc ggtattttcc aaaaatgctt
     8761 gttgaagctg gtggaatttt atatttccgt cgttcgcggt acgccgctgt tggttcagct
     8821 tgtgattgtg ttttacgggc tgccgtccgt cggcatctat atcaatccga ttcccgccgc
     8881 catcatcggc ttttcgctca atgtcggcgc atacgcttcc gaaaccatac gcgcggcgat
     8941 tttgtccgtg ccgaaagggc agtgggaagc aggtttctcc atcggtatga cctatatgca
     9001 gacgttccgc cgcatcgtcg caccgcaggc attccgcgtc gccgttccgc cgttgagcaa
     9061 cgagtttatc ggcttgttca aaaacacctc gcttgccacc gtggtaacgg taacggagct
     9121 tttccgtgtc gcacaggaaa cggcaaaccg cacttatgac tttttgcctg tctatatcga
     9181 agctgcattg gtttattggt gtttctgtaa agtgctgttt ttgattcagg cgcgtttgga
     9241 aaaacgtttc gaccgttatg tcgccaaata aggagttgtc atgattaaaa tccgcaatat
     9301 ccataagacc tttggcgaaa acaccatttt gcgcggcatc gatttggatg tgggcaaagg
     9361 gcaggtggtc gtcatcctcg ggccttccgg ctcgggtaaa acaacatttc tgcgctgcct
     9421 aaacgcgttg gaaatgcccg aagacggaca aatcgagttc gacaacgcgc ggccgttacg
     9481 cattgatttt tccaaaaaaa caagcaaaca cgatattttg gcactgcgcc gcaagtccgg
     9541 aatggtattc caacaataca acctcttccc gcataaaacc gtgttggaaa acgtgatgga
     9601 agggccggtg gttgccgtac agggcaagcc tgccgcccaa gcgcgcgaag aggctttgaa
     9661 actgctggaa aaagtcggct tgggcgataa agtggatttg tatccctacc agctttccgg
     9721 cggtcagcag cagcgtgtcg gtatcgcccg cgcactggcg attcagcctg aattgatgct
     9781 gtttgacgaa cccacttccg cgctggaccc cgagttggtg caagacgtgt tggacgccat
     9841 gaaggaattg gcgcgggaag gttggacgat ggtcgtcgtt acccacgaaa tcaagttcac
     9901 gctggaagtt gccacgaacg tcgtcgtgat ggacggcggc gttatcgtag agcagggcag
     9961 cccgaaagag ttgttcgacc acctcaaaca cgaacggacg cggagatttt taagccaaat
    10021 ccaatctgcc aagatttgat tggctacttg tgaaaaatgc cgtctgaaag tttcagacgg
    10081 cattttttct atttttacag aggccagatt aaatgcggat tgctttcgat gacggctttg
    10141 aatcggtttt gaatgcgctc gatggctgct tgcgtatccg cttcaaaacg caacaccaaa
    10201 atcggcgtgg tattggaagc acgcatcaga ccgaagccgt cgggaaattc aacgcgcagg
    10261 ccgtcgatgg tgatgatttc ggttgcgcct tcaaattcgg ctttggcggc gagttcttcg
    10321 ataacttgat gcccgttgct gccttcgggc agggagatgt tgagttcggg cgtggaaatg
    10381 ctttgcggca ggttgtccaa cacttcggac ggattgtcgg aggcggacag gatttccaag
    10441 aggcgtgcgc cggcatacag gccgtcgtcg aagccgaacc agcgttcttt aaagaaaacg
    10501 tgtccgctca tttcgccggc aaccagtgca ccggtttttt tcatagcgga tttgatgaag
    10561 ctgtggccgg ttttttccat tatggcttct ccgccgtgtt ctttaatcca cggggcaagc
    10621 aggcgtgtgg atttgacatc gaaaatgact ttcgcgccgg gattgcggtt caaaacgtct
    10681 tgggcgaaca gcatcagttg gcggtcggga taaataatgt tgccgtcttt ggtaaccacg
    10741 cccaagcggt cggcatcgcc gtcaaacgcc aagccgattt cggcatcgcc gtttttcagc
    10801 gcggcaatca aatcttgcag gttttccggt ttggaaggat cagggtggtg attagggaaa
    10861 ttgccgtcca cttcgcagaa aagttcggtc acttcgttgc ccaaaccttt gtagagtttg
    10921 ccggcaaacg cgccgcccac gccgttgccc gcgtcgatgg cgatgtttat cgggcgtttg
    10981 agtttgacgt gtccgacgat gtggtcgtgg tatgcgccgg agatgtcttt ttcggttacg
    11041 ctgccttgtt tgtcggcggc aacaaaaccg tctttctcaa caatagctaa aagttcttga
    11101 atggcttcgc ctgcgagcgt gtcgccgccg agcatcattt tgaaaccgtt gtaatcgggc
    11161 ggattgtggc tgccggtaat catcactccg ctgccgccgc attcgttgac ggctgcgaag
    11221 tagagcatag gagtggtaac catgccgaca ttgagtacgc cgataccgct gtcggtcagg
    11281 ccgcgttgga tgtgctccat cagttcgggg ccgctcaagc gtccgtcgcg tccgatcgcg
    11341 atgcgggcga tacctttttc ggcggctttg gcggcgatgg ccctgccgat gaaataagcg
    11401 gcatcgtcgg tcagggtttt gccgacgatg ccacggatgt cgtaggcttt gaagatgtcg
    11461 cgggtgatgc ttgccataag gtttcctttg tgtccgttta ggaaaaacgg gcgtatttta
    11521 acatagcggt atgccgtctg aaggcttgcg tccggttttc agacggcata gcccggttac
    11581 atcaaataac atgccgtctg aaataaaagc agccttttat gcaggctgct ttcggattgc
    11641 cggtttatac tgcttcggct ttaatgatga tgacaggttc ggtcggcaca tcgtcgtggt
    11701 agccgtggcg tttggtggat acgctttcga tggcatcgac aacgtcaaaa ccgtcaacga
    11761 ctttaccgaa tacggcatag ccccagtctt ggacgacggt tttgccgtac agctctttag
    11821 aacggaagtt taggaaagcg ttgtcggcag tgttgatgaa gaattgcgcg ccggcggaat
    11881 gggggtcgga agtgcgcgcc atggcgatgg tgtatttatc gttgggcagg ccgttggacg
    11941 cttcgttttg aatcggatcg cgggtttctt tttcgttcat gttttcatcc atgccgccgc
    12001 cttgaatcat gaagcctttg atgacgcggt ggaagattac gccgtcgtag aagccgtctt
    12061 tgacgtattg ctcgaagttt ttggcggtaa caggggcttt gtcgaaatcg agttcgattt
    12121 tgatgtcgcc tttgttggtg tgcaggataa tcatgggttt tcctttcgtt agaatccggt
    12181 tttgaattat tcgacaaatt gtgtctgaac gacaaacttc aaggtcgtct gaaaaatatt
    12241 ctttcagacg gtcttgttgt ttaggtcgat ggtttacatc agtacagcat aagcccacag
    12301 agcaaccaat actacgcata ggatgttcag cagtatgccg acattcatca tttctcgttg
    12361 tttgattaag cccgtgccga acacaatcgc gttaggcggc gtggcaaccg gcagcatgaa
    12421 ggcgcaagat gcggcgatgc cgatgacgaa taccaagact tgttcgggca gccccatctg
    12481 catagcgatg ccggagaaaa tcggtacaag cagggcggcg gaggcggtgt tgctggtgaa
    12541 ctcggtcaga aaaataatga aggcggcgac gatgagtatc accaaaaatg cgggcgcgcc
    12601 ggaaaaggtg gcggcaacct gctgtcccaa ggcttcggac gcgccggatg ttttcaacag
    12661 cgtgctcagg ctgatgccgc cgccgaagag catcaacacg ccccagtcgg tattgcgggc
    12721 gacttccttc cattgcgcca cgccgaagac gacgacggcg acggcggcac tcagggcgat
    12781 aactgtgtcg ggattggaaa tgccgaaggc ggttttgatt ttggagccga atatccacgc
    12841 ggcggctgtg gcaaggaaaa tcaacagcgc gatcatgcgg tgcagcgtcc aagggatgga
    12901 ttcggctttg atttccacgc gttcgttcaa attaggtttg aggatgacgt acagggagag
    12961 cagcatcaag ggcagaatca acagcatcat cggcaggccg agcttcatcc agccgacgaa
    13021 gtccagattt agggctttgg cggcaatcag gttgggcggc gagccgacga gcgtgcccaa
    13081 gccgccgatg ctggcgcaat aggcgatgcc gagcaggagg aagacgtagg ttttgtgttc
    13141 tttttcccgg tcgaggtggc tcagcatacc cattgctaga ggcagcatca tcgcggcggt
    13201 ggcggtgttg ctgatccaca tggacagaaa ggcagtaacg aggaacaaca tcaaaaccgc
    13261 cactttcata ttgccgcgcg acaggcgcaa caggctgacg gcgattttac ggtccagccg
    13321 ctgcatatgc agggcggtgg caagcgcgaa gccgccgaaa aaaatgtaga taatcgggtt
    13381 ggaaaaatca gccatcgcct ttttgatgtc catgtcgggg aaaccgagta cgacggcgag
    13441 aatcggcacc atcagtgcgg ttacggtaat gtggacggct tcggtaaacc aaagtgcggc
    13501 aacgaaaatc agcagcgcga tacctttatt ggcatcgggg ctgtaaggca ggatgtggta
    13561 aatgccgaaa cagacgacgg cggaaataat ggtggtcagc aaacccttaa agtcggtaat
    13621 cggcttctgc gcactgagca gctcgacgtt ttcgggatgc tgggttttgt cctttgcatg
    13681 caggttcatg aatgctcctt taaggcaaca aaatcggttt ttcttttgtg tcgtgcaatc
    13741 cgaaacggtt tgcgaaaccg tcggttctgc aaccgcttcg agtatatttg taattcggtg
    13801 tagtgtaaat atattgtaaa cgatttgtcg gttttgttta tgagatggga ttgatatgta
    13861 aggggaaaaa caggatgcat cagggggatt catcctgttt gggagaaata aaccgattta
    13921 gtccgggcgg caggcagaac gcgcggcaaa gagggcaagg gctgcgcccg tcaggtcggc
    13981 aaggacatcg cccaaactgc cggttcgcgt tgcagtaaac catgcctgcg cgcattcgct
    14041 gccgacggca aaacagaagg cgaacgcaat caggctgcgg taggggatgg gaagttttcc
    14101 ggttttgaat gctttggcca gaagcaagat ttgtgcgaaa aacagggcaa ggtgtgctgc
    14161 tttgtcgaaa tgtggaaacg gcggcggcgc ggtgtcggca gctttgaaga gcagcgaata
    14221 gatgccgccg gcaaaccaca atgcggaaag caggctgaag cggttgcgcg gcagcttcat
    14281 gccgcctcct cgtccagcca cgtttggcag attttggaca ggcgcaggaa tttgccgccg
    14341 cgtgcggcaa gtatgtcgcg ccattgtgcc acttcttcgg cggacggtgc gtcgtcgatg
    14401 ctgcattcgt acagcaggaa atcgagggtt tcttcgatga cggggatgga ttcggtttgg
    14461 ataagctgct tgagttcggt catgactgtt cggatacgga aatcgggaaa atgccgtctg
    14521 aaagggcttc agacggcatt ggattatttg ctgtgcagga agcgcgttgc ctcttcccat
    14581 ttgccggcaa ggatgtcggg tacggcctgc agggatttgg cgacggcatc gtcgatttgc
    14641 cggcggtgtt ccgcgctcgg tttgttcaag acgtagccga cgacgaggtt gcggtcgccg
    14701 gggtggccga tgccgaggcg caggcggtaa tagtctgccg tgccgagttt tgcctgaatg
    14761 tctttcaagc cgttgtgtcc gccgttgccg ccgccgagtt tgaatttgat ccgtccgcaa
    14821 gggatgtcga gttcgtcgtg gacgacgagg atttcttcgg gtttgatttt gtagaactgt
    14881 gcaagcgcgg caaccgcctg tccggaacgg ttcatgaacg tggccggttt gagcagccaa
    14941 acatcgccgt cgggcagggc ggcgcgggca acttcgccga agaatttttt ttcttcttta
    15001 aacgaagcct tccatttcca cgccagttcg tcgaggaacc aaaagcccgc attgtggcgg
    15061 gtctgttcgt attctttgcc cgggttgccc aagccgacaa ccattttgat tgtgtttgac
    15121 atgatatttt ccgtgtttct gtcgaatgcc gtctgaaggc ttcagacggc atggttattc
    15181 ttcttgattt tgaacgcgtt tgcggcgcgc ttctttgggg tcgatcaaca gcgggcggta
    15241 cacttcgatg cggtcgccgt cgcgcagcgg cgtgtcgtct ttgacggctt tgccgaaaat
    15301 gcccaaaggc gcggaatgca ggtttaaatt ttcaaatatg ccgtccaaac cgctttgcag
    15361 tgcggcggtg cggacggttg ttccctcggc aagctgcatg gttttcaaaa cctgtcggtc
    15421 gggcagaccg tacacaatct caatttcaag cataacggcg gtctgcctct ttgacgaacg
    15481 cttcgaccag cgtggcggaa aggtggttga agacggggga aattaaggcg gacaaaacgg
    15541 cattggaaaa atcgtattcc aaattgaatt cgattttgca catatcgtcg cccaaatcga
    15601 taaatttcca cgttccacgt aaggttttga acgggccttc gagcagttcc atacggattt
    15661 ccctgccggg gatgttgcgg ttgtgcgtgg caaacgattg gcgaacgcgc atataatcca
    15721 taaacagccg cgccttcagt tcgttgccgc tacgcccgat gacctcggtc ttgctgtacc
    15781 acggcagaaa gtgcgggtaa tcctcaacct tgtcgaccag ctcgaacatt ttgtccgcgc
    15841 cgtgcagcac caagatgttt ttttcaactt ttttcacggg catcaccaat gcgggcgtgt
    15901 attcgaaggg cggtattata gcggtttaat tttaaaccgg tacagccttg ccccgttctg
    15961 atttcaattt aaaccgccat atcgcagatt cgcaaaaacc gatacggctt tggcgtttca
    16021 gacggcattg agcggaaaat gccgtctgaa aacgggatgg gaaaacggca gcttgcgggc
    16081 tgccgtttgt tcgggaagtt aagccttgtt ttcaggctgc tgcccggact ctgtcgaggc
    16141 ggtgtctgcc ggggcaggtg cttcggtttt ttcccgagtc ggagcgtgcg gcgcgttatc
    16201 ttccgccgcg tccgcattct cgcgcaggtt gtggctgtaa tccttgggcg ggctgggttg
    16261 tttgcccgcc ataatttcca gtacctgatc gcggtctatg gtttcccatt ccatcagggc
    16321 tttgcacatc gtttccatct tgtcgcggtt ttcatcgagg attttgtagg caacctgata
    16381 ctgctcgtcc aaaatccggc ggatttccgc gtcgatgtcc tgctgggttt tctcggaaat
    16441 gttttgcgaa cgggttacgc tgcgccccaa gaagacttcg ccttcgtttt ccgcataaac
    16501 catcacgccc attttgtcgc tcatgccgta gcgcgttacc atttcgcgtg ccatttgggt
    16561 tgcgcgttca aagtcgtttg atgcaccggt ggagatgcgt ccgacgaaga tgtcttcggc
    16621 aatccgtccg ccgaacagga tggagagctg gctcaacatc tgatctttat acatactgat
    16681 gcggtcgcgc tccggaagct gccaagtcag acccagcgca cgtccgcgcg gcataatggt
    16741 tactttgtgg acggggtcgg taaagggcag gctttcggca acaatcgcgt gtccggactc
    16801 atgatacgcc gtcgcacgtt tttcgtcttc gtgcatcacc atactgcggc gttccggacc
    16861 catatagatt ttgtctttgg cgtcttcaaa atcgctttga tcgactttga ctttgttgcg
    16921 gcggccggca aacagggcgg cttcgttgac caggttcgcc aaatccgcgc cggaaaaacc
    16981 gggcgtgccg cgcgcgaggg acaataaatc cacggattcg tccaaaggca cttttttaga
    17041 atggacgttc aaaatctgtt cgcgcccccg gatgtccggc agggggacga cgacttggcg
    17101 gtcgaagcgg ccggggcgtt gcagcgcagg atcgagtacg tcggggcggt tggttgccgc
    17161 aatcacaatt acagtctgat tgctctcaaa accgtccatt tcaaccaata attggtttaa
    17221 tgtttgctcg cgctcatcat tgccgccgcc caaacctgcg ccgcgttggc ggcctaccgc
    17281 gtcaatctcg tcgataaaga taatgcatgg ggcgtttttc tttgcctgct cgaacatatc
    17341 gcggacgcgg cttgcaccga caccgacgaa catttcgaca aaatcggaac cggaaatgct
    17401 gaagaacggc acgccggcct cgcctgcaat ggctttcgcc aagagtgttt taccggttcc
    17461 cgggctgccc gccagcagga tgccgcgcgg aacacggccg ccgaggcttt gatagcggtt
    17521 cggtgctttg aggtaatcga cgatttcctg cacttcttct ttggcttcgt cgcagccggc
    17581 gacatcggca aaggtaactt tgttggcatc tttgtccagc aggcgggcgc ggcttttgcc
    17641 gaaggagaat gcgccgcctt ttccgccgcc gcccgcctgc atacgcataa agtagaacca
    17701 tgcgccaatc agcagcagga cgggcagcag gctgtaaaac agggcagtca gcgcgctcgg
    17761 tttttcttcc ggcgttactt ttacgcggac gtttttgttc aaaagggttt gaatcaggtt
    17821 gtcatccaag ggcgcgttgg tgaagaaggt gcttttgtcg gtgcgctcgc ctttaatcag
    17881 gtaaccgctg acgacggatc cttcgatgtt gacgccggat acttcgccgt tgttgacctg
    17941 tcggatgaac tgagaatatt cgatttgccc gttgtcttct tttttgccgt ccaaagcgtt
    18001 gaacgcagcc atcaggccga tacccaaggc gacccagaca aggattgatt taaaggtgtt
    18061 ccccacttag caaggctcca taattgaggt gtaaaacgga aatgattgta aagcacgccg
    18121 tctgtattgt cagcgtttat ttttgcccaa taaataaatc tcactggagc gattgcgcga
    18181 ggcttcgggt ttgcgcgtct gcacagtgcc gaaaatttcg cgcatggctg ccatgtattc
    18241 ctgatagcct gcaccctgaa agactttgac caaaaagctg ccgccggttt tcaggtgttg
    18301 cgaggcgaag tccaaagcca gttcgcacag ataaaagctg cgtgcctgat cgcttacggc
    18361 gtttcccgac atattgggcg ccatatcgca gattacaagg tcgagcgggc ggttgtccaa
    18421 caaggtttcg aattgcgcca aaacgtcgtt ctcgcggaag tcgccctgaa tgaaggacac
    18481 gccccctatg gcttccatag gcaggatgtc caaggcgaaa accgctccgg aagtacccgt
    18541 cagcttggcg gcaacctgcg accagcttcc cggcgcgctg cccaagtcgg caagtaccgt
    18601 gccgggtttg attatcttgt ctttttcgtt gatttccaaa agtttgtatg cggcacgggc
    18661 gcggtagccg tctttttgcg ccatatggac gtagtggtcg ttgatgtgtt cgtgcagcca
    18721 cgcttttgag gatttggaac gtacagccat agtggttcgc gggtcggaat gtaaacggtg
    18781 tattgtacgt taattttgcc gttgccgtgc caaatcgcgt acaatgctgc attatccttt
    18841 ttattcaaac agtaggaaaa tgacggatac caaattgaac accaaagaaa ttttggaact
    18901 gaaagcgcgc gcgcaccatc tccatcctgt tgtgatggtc ggtcagcagg gtctgacgga
    18961 cgcagtcatc aaggaaaccg atgcggcact gacggcgcat gagctgatta aagtgcgcgt
    19021 attcggcgac gaccgtgccg agcgtatcga aatctgcaat accttgtgtg aggcggttga
    19081 cgcgcaacct gttcggcata tcgggaaact tttggtattg tggcgtaaga atatcgaggc
    19141 ctgacagcct gaagtggttg ttttgctatt gttctttcaa tgggcggcac gctgtccttc
    19201 ggcgcggcat ttcggcgtgc cgaaaccctt tccggtgaaa acggattttg attaccgctc
    19261 gatgccgtct gcaagttgcg gcggcttccg tatgatttga attgttgaca ggatgattgg
    19321 agggcttatg caatttcctt accgcaatgt tccggcttcg cgtatgcgcc gtatgcgcag
    19381 ggatgatttt tcacgccgcc tgatgcgcga gcatatgctg accgccgatg atttgattta
    19441 tccggtgttc gtattggagg gggcggcgcg cgaggaggat gtgccttcta tgccgggcgt
    19501 gaagcgtcag agtttggaca ggctgctgtt tacggcggaa gaggcggtga agctcggtat
    19561 tccgatgttg gcactctttc ccgtggttac ggcaaacaaa accgggcgtg cgcaggaggc
    19621 gtacaatccc gaaggactcg tgccgtcaac tgtccgagcc ttgcgcgaga ggtttcccga
    19681 actgggcatt atgacggatg tcgcgctcga tccttatacg gtgcacggtc aggacggact
    19741 gacggacgaa aatggttacg tgatgaatga tgaaaccgta gaagtcttgg tgaaacaggc
    19801 tttatgtcat gcagaggcgg gcacgcaggt cgttgctcct tccgatatga tggacgggcg
    19861 tatcggcgcc atccgcgagg ctttggagga tgccggacat atccatacgc ggattatggc
    19921 atattccgcc aaatatgctt ctgcattcta cggccctttc cgtgatgcgg taggcagttc
    19981 gggcaatttg ggaaaggcag ataaaaagac ctatcagatg gatcctgcaa ataccgatga
    20041 ggcgctgcat gaagtggcgc tcgatattca ggaaggtgcg gatatggtga tggtgaagcc
    20101 cggtttgccg tatttggacg ttgtccgccg cgtgaaggac gagttcggcg taccgactta
    20161 tgcctatcag gtttcgggcg aatatgcgat gttgcaggcg gcggttgcca acggctggct
    20221 ggacggcggc aaagtggttt tggaaagcct gctggcattc aaacgtgcgg gtgcggacgg
    20281 gattttgacc tattacgcca ttgaggcggc aaagatgctg aagcgttgat atttgattcg
    20341 caatattgaa atgccgtctg aaactatggt ttcagacggc attttttacg gtttacaaag
    20401 ttgtatcgag tgcggcggaa atatcgttcc aaatatcatc tacgtcttca ataccgatgg
    20461 agaagcgcag gagtccgacc ctgatgccca tttccatttt cacattatgc ggcacgccgc
    20521 tgtgagattg ggaatagcaa tggttgacca gactttccac gccgccgagg ctggaggtca
    20581 ttttgaccag tttcatgttt ttaatcacgc tgtttgccgc ttcgcgcgtg tcgtttttga
    20641 gataaaccgt aaccacgccg ccgatgcctt tgggcatttg cgcttgggcg agtgcgtaat
    20701 gttcgtgaga cggcaggccg ggatggaaca ctttttcaat ggcaggatgg gcttccaaac
    20761 ggcgcgcaat ttcgagtgcg tttttgcagt gtgcctccat acgcagggca agcgttttga
    20821 tgccgcgcaa caccagccag cagtccgtcg ggcctgcaat cgcgccggta tgcaccatca
    20881 tatcgtgcaa aggctgagcc agctctttgg ttttggcaac gacgatgccc atcaatacgt
    20941 cggaatggcc gcacaaatat ttggtagcgg aatggaatgc aaaatcgcaa cccatatcca
    21001 agggctgttg cagatacggc gtggcaaagg tgttgtcgat gccgaccagc gcgtcggctg
    21061 ctttggcttt cgctgcaagg gctttgatgt ctaccaagcg taaaagcgga ttggacggca
    21121 tttccagcca aaccagtttg acattgtgcg ctttgagcaa ttcgtccaaa ttatcgggat
    21181 tgcccaaatc ggcaaaaaca acgttcaccc cccatttttg ataaacatcg accaataagt
    21241 catatgcgcc gccgtaaata tcggcgaccg cgacgatggt atcgcccggg cgcaggaagg
    21301 tacgccacac ggcatcaatc cccgccatac cgctggaaaa cgcaaaacct gccgcaccgt
    21361 gttccaaatc ggcaacggtg tcttccaaaa tctgacgggt cgggttgctc aggcgcgaat
    21421 aacggtaagg cacattttcg ccgatttcgt gcagcgcaaa catactgttt tgataaatcg
    21481 gcggcatcag cgcgcggttg tgttcgtcgc aatcgtagcc ggaatggatg gcacgggtcg
    21541 ggaatttcat ctgtttctgc cttgtaagat atggataagt cgggatttta tcactatccc
    21601 gaaaatagaa acaatcaatg cagccgccaa aagcaagtga tataatctcg catttgcagg
    21661 caggacggcc gacagcgttt tttcagacgg catctgcctg ccatacaata cacacatctc
    21721 aataattatc tgaatattaa ggacaaacaa cgcatgaacg ccattgcaga cgtgcaatcc
    21781 agcagagatt tgcgcaacct gccgatcaat caggtcggca ttaaagacct gcgcttcccg
    21841 attaccctga aaaccgccga aggaacacaa tccacggttg ctcgcctgac gatgacggtt
    21901 tatctgcccg ccgagcagaa agggacgcat atgtcgcgct ttgtcgcatt gatggagcaa
    21961 cataccgaag tcttggattt cgcacaattg cataggctga ctgccgaaat ggtcgcgctt
    22021 ttggattccc gcgccggcaa aatcagcgtt tcttttccgt ttttccgcaa gaaaaccgcg
    22081 ccggtatccg gtatccggtc cttactggat tatgatgtca gcctcacggg tgaaatgaaa
    22141 gacggggcat acggccacag tatgaaggtc atgattcccg taacctcgct ttgcccgtgt
    22201 tccaaagaaa tttcccaata tggcgcgcat aatcagcgtt cgcacgttac cgtcagcctg
    22261 acttccgatg ccgaagtcgg tatcgaggaa gttatcgatt atgtggaaac gcaggcgagc
    22321 tgccaactct acggcctgct caaacgcccc gatgaaaaat acgttaccga aaaggcctac
    22381 gaaaacccga aattcgtgga agatatggtg cgcgatgtcg ctacttcgct gattgccgac
    22441 aaacgcatca agagtttcgt cgtcgagagc gagaatttcg agtctatcca caaccattcg
    22501 gcttatgcct atatcgccta cccgtaggcg cgtttgcgat gtccaaatgc cgtctgaaag
    22561 gcattgggga gttattcccg aatctgccgc cgctttgttt tcccagccgt agagagatgg
    22621 gtaaaatccg ggtgaagctt gatggcgggg acggcttata gggtgcggca gtgccgtatc
    22681 ggtttgaaat caatttgcaa tcaaagtaat aaaaggatgc acgatgacag tattaagcaa
    22741 agagcaggtt ctatccgcat ttaaaaaccg caaatcgtgc cggcattacg atgcggcacg
    22801 taaaatcagt gccgaggatt ttcagtttat tttagaactc gggcgtttgt cgcccagttc
    22861 ggtcggttcg gagccttggc ggtttgttgt ggttcaaaac cccgaaatcc gacaggcaat
    22921 caagctgttt tcttggggta tggcggatgc tttggatacc gccagtcatt tggtggtgtt
    22981 tttggcgaag aaaaatgccc gcttcgacag cccgtttatg ttggaaagtc tcaaacggcg
    23041 cggcgttacc gaaccggatg ccgtagaaaa atctttggcg aggtatcagg cgtttcaagc
    23101 tgacgacatc aagattttgg acgattcgcg cgccttgttt gactggtgct gccgccagac
    23161 ctatatcgcg ttagccaaca tgatgacggg tgcggcgatg gcgggtatcg attcctgccc
    23221 ggtggaaggt ttcaactatg ccgatatgga acgcgtattg tccgggcagt tcggtttgtt
    23281 cgatgcggca gaatggggcg tgtccgttgc cgcgacattc ggctaccggg ttcaggaaat
    23341 cgtcacgaaa gcgcgcaggc ccttggaaga aaccgttatt tgggcataag gcagtgccgt
    23401 ctgaaaacgc aaggattttc agacggcatt ttttaatgct tatttaaaac gattcgccgg
    23461 gagcgagata acgccatttg ccgggcggca gcctgccgag tttgaccttg cccatgcgga
    23521 tgcgtttcag cccgacgacg cgcagtccga ccagttcgca catacggcgg atttgccgct
    23581 ttttaccctg tttcaacacg aagcgcagtt ggtcttcatt ttgccattct actttggcgg
    23641 gacgcagttt atcaccgtcc aaactcaatc cgtgattcag taaggcaagt cctttttcgt
    23701 ccaatttgcc gcgcacgcgc accaaatatt ctttttcact gccgctgttt tcgccgataa
    23761 gctgcttggc gatacggccg tcttgcgtca acaccagcaa tccgactgaa tcgatatcga
    23821 gcctgccggc gggggcaaga ccgattttgt gtttcggatc gaaactgatg cggctggtgt
    23881 cgccttccca gcaattttca ggggtaatca gttcggcggc ggatttatag cctttttccg
    23941 cttgtgcgct gacatagccg acgggtttgt tcagcaggat ggtaatgcgt gccgcctgct
    24001 gttcgtgggc tttcttgttc agttcgatac ggtctgccgg tgaaactttc tgaccgagta
    24061 cggcggtttt gccgttgacc gttacccagc cctgttcgat atggccgtcg gcttcgcggc
    24121 gcgaacaaag ccccagttgt gccatacgtt tggagaggcg tatggtatct tctgtatggt
    24181 cggaggaaat tttggaattc atgggtactt tcgggtgaag gccggcttag actaattgct
    24241 cgccccagtg cagtttttgg cgcagggttt tgaaatattg gtagtcggtc gggtgtagga
    24301 tgcgtaaggg attgtggtag cggcggatga tgatgcggtc gaggttttgc acgtcgatga
    24361 aggattgtcc gtcgaaatga acgcgcgcgt cgccgccttg ggtaacgagg atttcgattt
    24421 cggacgtgtc tggaatggcg atggggcggt tagtcataga ttgcgggcag atggggacga
    24481 gcgtgaaggc gtgtaatcct gcctgcatga ttgggccgcc ggcggcaagc gaataggcgg
    24541 tcgatccggt gggggtggag acaatcagcc cgtccgaacg ttgtgtatag acgaattcct
    24601 gattgacgaa gacttcaaat tcaatcatct gtccggcacc gccgcgggag aggacggcat
    24661 cgttgagggc gagggcgcgt tcggcggttt tgccttcgcg gatcagtgcg gcttcaatca
    24721 ggatgcgctc ttcggcaagg tattttcctt ctaaaacggg caatagcttg tccgtcatat
    24781 attcgcgggg aatttgggta agaaagccca aatgaccttg gttgatgccg ataatcggaa
    24841 cggcgcgggg ggtaatttcg cgggcggcag agagaaaggt gccgtctccg cctaagacgg
    24901 cgaccaggtc gcagtattgc cccagttcgg tcttgttgac gatatggcag ccgtcggtgt
    24961 cttgggtata gatgcagcat tcccttacgc cgacttcgtc gagatagacg gtaaagccgt
    25021 gctgcttcaa aaaggtaatc agcgtgtgtg cggtatcttg gatgtcgggc gtgttggggc
    25081 gggttacgat accgatgttg tgaaaagggc tgttcatgtc ggatgccgtc tgaaggttag
    25141 tctatccaaa tgtcgcgttc gagcctgtcg aggcgttcgt tgaggcgttc cacgccgtcg
    25201 cgcagtctgc ttatttcgtc gaggcagtcg gcaagggctt cgttgccggt gtttgcggac
    25261 tcgggttcgc gggaaaatcc gccgatttgt tcggcgatgt tcctgccgat ttgtttgatg
    25321 ccgtgtccga tgtcggtggc acggctgccg atgcctgcct gcgtgccgaa aatccgtgcc
    25381 aattcgtccg atgcgcggga acgcaggctg ccgagcaggg acagtaccgc gatgccgagg
    25441 atgaggtcgc cttcgagcct gatgtcgcca gccccgggtt cgccgccttg gaggattttc
    25501 cgtatcgcgc tgttgcggaa ggtaatttcg gtgtctgcaa agccgtttcc cgccgagagc
    25561 aaaccgtctt ctgtgatgcg tcccgccagt ttcagcccgg caatgttcag ggtcagtgtt
    25621 ttgcctgcaa aggaggtaag ttccgagcgg ctgtccgggc tttgcagaat caggcggttg
    25681 atgatgggga ggagggcgga catatttttt gattggggcg gagaatgcct gttgttgctg
    25741 ttgccgctta ttttacaggc ttaagccgtt atcgcaaacg gtacggatga ttttgcccac
    25801 gctgtcgtcg ggtgagaaat ccggcgcggg caggccgagt ttggcggctt cttccgtata
    25861 aaattcgcgg cgtgtcgggt ggcgcggttc gataatgttt ttcagccgcc tgccgccggg
    25921 gttggatgcc gtctgaaaca ggttttcgac ggcgatgtcg cggtggacga tattgacagg
    25981 ccggttgccg ccttggatgt tttgcttttg cacgaggcgg ctgacgggat ggcgttcggc
    26041 gcaataaagc ccgcccagcc gcaggatgtc gatgttcgga acgccgttgt cgagcaggtg
    26101 ttgttcggcg gcaaggattt gtcgggcgga ctcggtttgc ggatcgggtg cggcggtttc
    26161 gtcgcattct cgcgctttat cgccgtaaac gcttgtgctg cttgtgaaaa tcaggtgttg
    26221 cacgttgcac gcccgggcaa gctctgccca ttgtttgacg gtgtcggcgt aatgcgccaa
    26281 aaaggagggc ggcagcagga agaaccagac gggtttgtcg gcgtgatgcc gccacaatgc
    26341 cgtctgaaag gcatcggtac ggttgaggtc gaaggtgtcg aggtgtatgg gcaggttgat
    26401 gtcgtccgaa gtcaggctgc gttttatggc ggcgacgcgg ctgccgtgtc ggtaaaactt
    26461 ttgtgccagc ggcaggccga ggtaacctag gcctgtgatg gagatatggg gcggggggac
    26521 tgcgcgcatt cgctgatacc gtcgggtaag tgccgtctga aggctgattc ggacgttatg
    26581 ggtttacggg ttgccgttgc cgattttgcg gtcgtattcc gcgcggtgtt cgggcaaaag
    26641 ataggggcga aggaggctta aggtacgacg gacgccgcgt gctttggagt cttcggtcag
    26701 cttggtacgg ccgcgcaggg aactgtcgaa gtcgaaccag tatttcgtaa ccatccacat
    26761 attgacggcg aggtcgttca tagcggtttg gtcggcgttg atgacgttca gcccgttgag
    26821 ctgggtcagc agtttgacta gaagcggcga gactttggct tgggtaaagg tattgtgttc
    26881 gcccaacaat tcggcactgc gcgcaagtag ggtgtttacg tcgctaaaga ggaagcggta
    26941 ttcccacatg acatcgtaaa tgcctgccat atagttgata gagtcttcca catcagaggg
    27001 caacacggct tcattcaggt atgccaacag ggcttcgctg taacgtttga agagttggac
    27061 gatgatttcg tctttgttgc ggaagtggta ataaagattg cccggactga tgcccaaatg
    27121 cgccgcgata tggttggtgc tgatgttgcg ctcgccttcc tcgttgaaaa gcgcaaggct
    27181 ggcgtcgatg atgcgggtgt aagtattggt ttttgcaatg cgggtcacgg gcatactcct
    27241 tggatttaca ggctgaacga agtgggcagc caattgggtt cggcgtgtaa ttctacctga
    27301 aatcgagtaa atactgtatt gcagacgtgt tgcgccagtt tccagatgtc gtctgaagag
    27361 gcattgtttt tgttcgccaa aaccagagcc tgccggtcat gtaccgccgc accgccgatt
    27421 tggaagcctt tcagacggca ttggtcgatc agccagccgg cggcgagttt gaccgaaccg
    27481 tcgggctgcg gatagagcgg catatcggga tgccgctgca acagggtggc ggctttttcc
    27541 gcgctgacga cggggttttt aaagaaactg ccgacattgc ccaaaacttt cgggtcgggt
    27601 aatttgctgt tgcggatggc ggataccgca tcggaaacgt ctttcgccgt tgcctcccta
    27661 cctgcactca attcggcaac cttcgccgcc aaatcgccgt aacccaagtt cggcacaaaa
    27721 tgcgttttta atgcaaatac gaccgaaaca atcacataac gccctttgcc ttcctgcttg
    27781 aacaggcttt cgcggtaggc gaagcggcag tcggcattgg aaagggttac aaaggtttcc
    27841 gtatccaaat cgaagcagcg cacgctgtga atcacatctt tcgcctccac gccgtatgcg
    27901 ccgatgttct gcacgggcga cgcgccgacc gtacccggaa tcaggctcag gttttccaaa
    27961 ccgctcaaac ccagcgcgac ggtgtgcaac acgaaatcgt gccaaatttc gcccgcctgc
    28021 gcttcaatca gaaccatgcc gtctgagcgc gcaatctcgc gtatgccctt gttttccata
    28081 tggacgacca agccgtcgta atcctgcatc aaaaggatgt tgctgccgcc gcccagccat
    28141 aaaacagtat cgcggtcgaa ctcgggcagt cggacgatgt cgcgcaattc gtcggcgtgt
    28201 ttaagcgcga taaaggcccg ggcttgggcg tgcaggccga aagtgttgta gggggtgagg
    28261 tcggttcggt atcggatggg ttgcatggtt tgaactttaa ttgtatttga attgaagtgt
    28321 gctgcgtttt cagacggcct cgtgtgattt gaccaactcc atactgtatt tttccaagca
    28381 ccacaccaag gcgacggctg cgatggtgag cgaggcaatc aatgccgtcc agaagccgta
    28441 aatgcccata tcgaaacggt aggcgagcag atagcccggc agcaggccgc agccccagaa
    28501 ggcggcggcg tggatgaaca tcggcacctt ggtgactttg tagccgcgca gggcatagga
    28561 cgcgatacat tgggtgaagt ctgccggttg gaacaggccg gcgaacagca ggacggtgga
    28621 ggcgatgctt aaaactgccg gatcatcgtt gtacatgctt gccagcggcg aacggaataa
    28681 taccaaggaa agcacggtaa tcacggcgag cacccagccc gacaccagcg acactcctga
    28741 aatataacgc gcccgcgaaa attcgcgccg cccaagcgaa aagccgatgc gcaccgtccc
    28801 tgccgagccg acgctttgcg gaatcatata gagaatcccc gacaaactga tgccgacctg
    28861 ctgcgccgcc acataatcct cgccgaaagg cgcaatcaaa aacacgataa acgaaaacgc
    28921 gctggcttcc aaaaaataag acagcccgat gggcgcgccg attttccaaa tctgtttgaa
    28981 caccgcccaa tccggtttgc cgaatttcgc tgtcagtccg aacgggcgga agaatttttc
    29041 cttggcgata taaatccaca atgccagcgc gctgaaccaa aacaccgcca ttgtcgccac
    29101 gccgcaacct gcgccaccca aagcgggcat accgaatttg ccgtaaacga aaatatagtt
    29161 cagcggcacg ttcaacacaa acgccgcaaa gctgaccaac ataatcaggc gcgggcggtt
    29221 caggctggaa gcgtaggcgt gcagtgcgcg gtgtaccatt gccgccggca tcgccaagct
    29281 ggtgaacagc atatactgcg ccattgtgcc ttccacataa tcgctcaaag tcagccagtt
    29341 gcggaacggc gtaatcgccg cccacatcaa aatcatgccg aaaatcccca aaatcagccc
    29401 gaaccaaatc ccctgccgcc ccgtttcgcc tgcttcaccg gttttacccg cgccgtaaag
    29461 ctgggcaatc atcgggttca gcgccgccat aatgcccata aaggtaatat aaaccgtggc
    29521 aaacgcgctg ctgcccaaag ccaccgccgc caaatcttcc ttgcccgcac cgcccgccat
    29581 cacggtatcg acgaaaccga tgcccacctg cgcgacctgc gccaacagca tgggcagggc
    29641 aagggcggtc agcaggcgga tttctttcag gaagacggaa aaggaaaagc ggtcgaggtc
    29701 gagcagcata agtgttcagt caataaaaat gccgtctgaa acaaaaaaaa cggcagcggg
    29761 aagtgatgag aaataatggt gcacattata tagtaaaaaa ataccgtgcc gtcagacggc
    29821 ggatacgggg tatataaagt atattcagat tgtgtgtatt ttatggtaaa gtttggtttt
    29881 aacgacttga cgggattgag ccgtcggaca ggggctgttc ggattctgaa tcggaaggaa
    29941 gcatcgccgt tttgacagcg gcgtgatgcg ttgcggcaaa gatgccgtct gattgcggat
    30001 cgggcagtct tttgtgttta caggataaaa cagaaggcag attctcatgc agacatggca
    30061 gcttccggaa catatcgccg acgtactgcc cacgaacgcg cggcagcttg aaagcgcgcg
    30121 cgagcagttg ttggcactgt tccgcgtaca cggttatgaa ctggtacagc ctccgctgat
    30181 ggagtacgca cattccctgc tgacgcatat cgatgcgggg ctttcactga aaaccatttt
    30241 ggtaacggac aggctcagcg gcaggcagtt gggcatacgc gccgacatca cgccgcaggt
    30301 ggcgcgtatc gatgcccatc ttttgtccgc caaccaaggg attaaccggt tgtgttatgc
    30361 cggtccggtg ttgcacgcgc agcccgacgg tctgccgaat atgcgcgaac ccttgcaggc
    30421 aggggcggaa atgtacggtt ttgccgacat ccgtggcgac atcgagctga tagaccttat
    30481 gctgaaaagc atgaaaattg ccgatatggg caaagtgctg ctttcgctgg ggcatatcgg
    30541 catattccgc gccttgtccg atgcggcgca tttggatgcg gggcagtccg caacgctgct
    30601 tgccttgatg caggataaag ataccgggtc ggtcgaagcg caggtcaagg cttggaagct
    30661 ggacggtatg tgggcaaaag cattctcgct gctgccgcgc ctgtacggcg ggcgtgaagt
    30721 gttgtccgac gcgcgcggac ggttgccgga tttgtcggcg gtcggcggcg cgttggacga
    30781 attgcaggcg gtgtgcgacg cattccccga taatgaaatc catatcgact tgtccgagct
    30841 gcgcgtcgac aattaccaca cgggcttgct gtatgccgcc tatgccgccg atttccacga
    30901 cgcggtcgcg cgcggcgggc gttatgacgg attgggcgga tatttcggca gggcgcgccc
    30961 ggcaacggga ttcagtttcg acttacgcag ctttatcggg cgtttgcccg ccgtcgaacg
    31021 gcagcccgcc gtgttggtcg atgcggaaga tgcagaagcg gcgcgcgaag cggtcgaagc
    31081 cttgcgtgaa caagggcagt gtgtcgtgat tgactacggt atcggacaca atgtttcgga
    31141 agagcttgca ggccgtctga aaaagacgga cggcgtttgg caggtcgtga aacgctaaat
    31201 acccgttcat agcggatgaa aggcaaaccg gggcggggcg caaagccgca ccggtttggg
    31261 gattttccgc aataattttt aatatcgata ggttatatgg ctatggctaa aaatgttgta
    31321 gtaatcggcg cacagtgggg cgacgagggt aaaggtaaaa tcgttgactg gctggcggaa
    31381 gaagccggcg gcgtcgtgcg cttccaaggc ggccacaatg cgggccatac cttggtcgtc
    31441 ggcggcaaaa aaaccatttt gcgcctgatt ccgagcggta tcctgcacga aggattggat
    31501 tgcttcatcg gttcgggcgt tgtcgtttcc cccgaagccc tgttgggcga aattgacgag
    31561 ttgaacgcgg caggcgtgaa aaacgtcgaa ggccgtctga aaatcgcgcc gacctgcccg
    31621 ctgatcctgc cttaccacat cgcgctcgac caagcccgcg aagcatcgcg cggcaaaggc
    31681 aaaatcggca cgaccggccg cggcatcggc cctgcctacg aagacaaagt ggcacgccgc
    31741 gccattcgcg ccgccgattt gctgcatcct gaaaaactgc gtgaaaaact ggatgccgtc
    31801 cttgcctatt acaacgtcca actccaatac ctgcacaacg ccggaccggt taaagcggaa
    31861 gacgtgatgg cggttatcga aaaagtcgcg ccgcgcattg cgccgatgat tgccgacgtg
    31921 tcccgcgtgt tgaacgaaaa aaacaaaaac ggcgaaaaac tgctgtttga aggcgcgcaa
    31981 ggtgcgttgt tggacatcga ctacggcact taccccttcg ttacctcgtc caactgtctc
    32041 gccggcgcgg cttcggcagg cgcgggcgta ggtcctcaaa tgctggatta tgttttgggc
    32101 atcgtcaaag cctataccac gcgcgtcggt tcgggcccgt tcccgaccga attgttcgac
    32161 gaagtaggcg caggtttggc ggagcgcgga cacgaattcg gttcggtaac cggccgcgcg
    32221 cgccgctgcg gctggtttga tgccgccgcc ctgaaacgct ccatccaaat caacggcatt
    32281 tccggtatgt gcattaccaa actcgatgta atggacggcg ttgaaaccat caatatctgc
    32341 gtcggctacg aattgccagg cggcggcaaa accgacatcc tgccttgcgg ttcggatgcg
    32401 gtggaaacct gcaaaccgat ttacgaaacc atgcccggct ggcgcgaatc gactgtcggc
    32461 gtgaaaagct acgacgcatt gcctgccaat gccaaagcat atttgaaacg gattgaagaa
    32521 gtctgcggcg cgccggtcgc catcgtctcc accggccccg accgcgaaga aacgattgtg
    32581 ctgcatcatc cgttcgcata aggttttgca gtaaaaatgc cgtctgaagc cctaattggt
    32641 ttcagacggc atttttaccg ctgttttatc tgatattttc tgtccggaca cggtttgtcg
    32701 gggatatatc aatgcggcgt atccggtgcg gaaatggata cggttggtgc cggtatggaa
    32761 acctgatgtt ttcagtcggc atatacgaaa aaaccgtact gcttgtgcgt acggtttttt
    32821 gtcgtttcaa atcggtttaa agcgatttga ggcgggcgat tcggttgtcc agcgaagggt
    32881 gggtgctgag cagggagtcg cgcgtatctc cggcgatgcc cattgcgttc atttcttcgg
    32941 gcaaatcgac cgggttgcct ttaagccttt gcagggcgga aatcattttc ggtgcgccga
    33001 ccagttttgc cgcgcccgcg tcggcgcggt attcgcgttg gcggctgaac cacatgacaa
    33061 tcaggctggc aaggaagccg aacaggattt ggaataccat gctgactagg aaataagttc
    33121 cctgggactg gctgccgtcg ttgtttcggg caatcaggtt ggcaataatg cgcgacagga
    33181 acacgacaaa ggtattgacc acgccttgaa tcagcgtcag cgtaaccatg tcgccgttgc
    33241 cgacgtgcgc catttcgtgc gccaacacgg cttccacttc gtcgcgcgtc atatggtcga
    33301 gcaaaccggt gctgacggcg atcagggagc tgtttctcga tgcgcccgtg gcaaaggcat
    33361 tgggttcggg ggagtggtag atggcgactt ctggcgtttt caggttccat tgccgcgctt
    33421 gggcttcgac agtgttcaga agccaggctt cttcttcggt gcgcggcgtg tcgatgactt
    33481 ccgcaccgac cgattgtttg gcgataaatt tggacatcag cagcgaaata atcgaaccag
    33541 tgaagccgac gacggcggaa tacgccaaca ggctgcccgc gccgccccgg ctgttgatgc
    33601 ccagaaccgc caaaacaatg ttgattacga ccaaaacagc gatattggta gccaaaaaca
    33661 gaaaaatgcg tttcacggat gttccttttt ggtagggtgt gatgttttga aattttgggg
    33721 attgtcccaa aaagttgccg ccttgtgaat atcaggctcg gcaaaggtat gcaaaacatt
    33781 tgcttgcaaa tggcagtttg tgcagttggt ttttgaacta ttgcgccaag ccgtgtagaa
    33841 tcgtaaacca tctgtttgat tccaataaac acatttaaaa ggatcacttc atgaaagcat
    33901 tacttttagg cgcaccgggc gcgggcaaag gcactcaggc gcaattcatc accgccgcgt
    33961 tcggcattcc gcaaatctct accggcgaca tgctccgtgc cgcgattaag gcaggcacgc
    34021 ccttgggttt ggaagcgaaa aaaatcatcg atgaaggcgg tttggtgcgc gacgacatca
    34081 ttatcggcat ggtcaaagaa cgcatcgcgc aagacgactg caaaaacggt ttcctgttcg
    34141 acggtttccc gcgtacattg gcgcaagccg aagcgatggt ggaagcaggc gtgggtttgg
    34201 acgcggtcgt tgaaatcgac gtatccgaca gcgtgattgt cgaccgcatg agcggccgcc
    34261 gcgtgcattt ggcttccggc cgtacttacc acgttaccta caatccgcct aaaaccgaag
    34321 gcaaagacga cgtaaccggc gaagatttga ttcagcgcga cgacgacaaa gaagaaaccg
    34381 tgaaaaaacg tcttgccgtt taccacgagc aaaccgaagt tttggttgat ttttacagca
    34441 agctggaagg cgaacacgcg ccgaaataca tcaaagtcga cggtacccaa gcagtagaag
    34501 ctgtgaaagc cgaagtattg ggcgcattgg gcaaataaat cgaaaaggtc gtacccacgg
    34561 gcaggcttcg cactctgaaa acagaaaatc aggtttcaga cgacctgttt ttgataaaca
    34621 gcgtgttgca accgaaaaat aatcattcgg cgtcattccc gcgcaggcgg gaatccattt
    34681 ctgaatttgg gcaatcgctg tttaaatctg atgaactgag ttttatcaat ggattcccgc
    34741 ctgcgcggga atgacggctg atgtaccggt tcaaatttat ccgaaacagt ttgtcggggg
    34801 cttgagtccg cgtgggtcgg acatcaatgc ccggcctacg gtttgaattt acgttgtact
    34861 gcgaaaaaga cgataaaagt cgtctgaaaa ccgaaacgaa aacaccatga atcctttaat
    34921 caccgacttc caaactccgc aacaacgtac ccccgtcatc gttgcccttg attttgccaa
    34981 cgaaaaagac acgctcggat tcgtccgcaa ccttgatccg gcgttgtgcc aaatcaaaat
    35041 cggcaaagag ctgtttaccg cgacgggacg cagtttggcg gaaagcctga ttcatcaggg
    35101 tttcaaactc tttcttgatt tgaaatacca cgatattccc cacaccgtcg cgcaggcgtg
    35161 taaagtcgcc gccgatatgg gcgtgtggat ggtcgatatg cacgcgtcgg gcggcagacg
    35221 tatgatggaa gccgccgccg aagccgttgc cggatacggc acgaaaccgc tcttaatcgg
    35281 cgtaaccgtg ttgaccagca tggagcaaag cgatttggcg gaaatcggtt tgaacaccgc
    35341 ccctgaagaa caagtcatcc gtttggcaaa actggcacaa agttcgggct tggacggcgt
    35401 ggtctgttcc gcccaagaag ccgcgccgct gcgccgcgaa ttgggacggg attttgtctt
    35461 ggttacaccg ggtatccgct tggacgttgc cggcaacaac gacgaccaac gccgcattat
    35521 gacacccgcc gaagccttgg cggcgggttc gacttatctg gtgatgggcc gccccgttac
    35581 ccgagccgcc gatccggtcg ccgtattgcg cgaagtgaac cgcgtggcaa accttgaagc
    35641 aaactgattt tcaggcggtc ttacaggtta aggttgtctg aacaaaacac aacggaggca
    35701 atatgcccgc caagttccaa caagaaaccc tcaaatcccg tttcgcacaa gccaaagttc
    35761 ttgttgtcgg cgacgtgatg ctcgaccgct attggtttgg cgacgtgtcc cgaatttcgc
    35821 ccgaagcacc cgtgccggtg gcaaaaatcg gacgaatcga ccaacgggcg ggcggcgcgg
    35881 cgaacgttgc gcgcaacatc gcttcgttgg gcggcagggc agggctgttg tccgtaaccg
    35941 gcgacgacga agccgccgcc gcgctcgacg cgctgatggt gcaggacggc gtcgcctcct
    36001 atctgatgcg cgacaaacaa atcgccacca ccgtcaaact gcgcgtcgtc gcccgcaacc
    36061 agcagcttat ccgccttgat tttgaagaac atcccaaccg cgaagtgttg gaacaaatca
    36121 agcggaaata ccgtgaaatc ttgcccgaat acgacgtaat cattttttcg gactacggca
    36181 aaggcggtct gtcgcatatc tccgatatga tcgattgggc gaaacatgtc ggcaaaaccg
    36241 tcttaatcga ccccaaaggc gacgattacg aaaaatatgt cggcgcaact ctgattacgc
    36301 ccaactgcgc cgaattgaaa gaagtggttg gcagttggaa aaacgaaggc gatctgaccg
    36361 aaaaagcgca aaacctgcgc cgccacctcg acttgaccgc cgttttgctg acccggagcg
    36421 aagagggcat gaccctgttc agcgaaggcg aacccattta ccagcccacc cgcgcccaag
    36481 aagtttacga cgtgtccggc gcaggcgaca ccgtcattgc cggaatgggc ttggggctgg
    36541 cggcaggctg caccatgccc gaagccatgt accttgccaa tactgcggcc ggggttgtcg
    36601 tggcgaaact cggtacggcg gtttgctcgt ttgcagagtt ggttgaagca ctggacgggc
    36661 aataaatctt ttcagacggc atgaccatcc aatgccgtct gaaaccctca aaacaaagga
    36721 aaccgaatat gaccatcatc gtaacaggcg cggccggctt tatcggcagc aacatcgtca
    36781 aagccctcaa ccaacgcggc attaccgaca tcgtcgccgt cgataatctg acaaaaggcg
    36841 aaaaattcaa aaaccttgcc gagtgcgaaa tcgcccatta tctcgacaaa cacgaattta
    36901 tccgccaagt gagggaacac attttacctt atcaaaacat cgaagccgtt ttccatcaag
    36961 gcgcgtgttc cgatacgatg aaccacgacg gtttgtatat gatggaaaac aactaccagt
    37021 acacgctgga tttgttggac tggtgtcagg acgaacgcat ccccttcctt tatgcctcca
    37081 gtgcggcggt ttacggcaaa ggcgaaatct tccgcgaaga gcgcgaactc gaaaaaccgc
    37141 ttaatgtgta cggctactcc aaattcctgt ttgaccaagt attgcgccgc cgcatgaaag
    37201 aaggtctcac cgcccaagtc gtcggcttcc gctacttcaa cgtttacgga caacacgaac
    37261 aacacaaagg ccgcatggca tccgtcgcct tccaccattt ccaccaatac cgcgaacacg
    37321 gttacgtcaa cctgttcggc agcaacgacg gctacggcaa cggcgaacaa acccgcgact
    37381 tcgtcagcgt cgaagacgtc gccaaaatca acctctactt cttcgaccat cccgaacttt
    37441 ccggcatcta caacctcggc accggccgca gccaacagtt caacgaactc gccgccgccg
    37501 ccgtcaacgc ctgccgcgcc gccgaaggca aatctgaatt gagcttgaaa gagttggtag
    37561 aagaagaact tatccgctat atcccctttc ccgacgcgct caaaggcaaa taccagggct
    37621 tcacccaagc cgacatcacc aaattgcgcg aagccggata taaggaagaa tttttcgatg
    37681 tcaaagcagg tgtcaaccgc tacgtcaaat ggatgctgga aaatttggct taatttgaat
    37741 gcccgtaaaa aaatcgtctg aaaatatcag gcgattttga tttgtttaac tttatatatg
    37801 gatttcgatg atgaccgaaa tgcaacaacg cgcccaactg caccgccaaa tttggaaaat
    37861 cgccgacgaa gtacgcggcg cggtggatgg ctgggacttt aaacaatacg ttctcggcac
    37921 acttttctac cgctttatca gcgaaaactt caccgactat atgcaggccg gcgacagcag
    37981 cattgattac gccgctatgc cggacagcat catcacgccc gaaatcaaag acgatgccgt
    38041 caaagtcaaa ggctatttca tctaccccgg ccagcttttt tgcaatattg ccgccgaagc
    38101 ccatcaaaac gaagagctca acaccaagct gaaagaaatc tttaccgcga ttgaaagctc
    38161 cgcctccggc tacccgtccg aacaaggcat caaaggcttg tttgacgact tcgacaccac
    38221 cagcagccgg ctcggcagca ccgttgccga caaaaacaaa cgccttgccg ccgtccttaa
    38281 aggcgtggcg gaactcgatt tcggcaattt tgaagaccac cgcatcgacc ttttcggtga
    38341 tgcctacgaa tacctgattt ccaactacgc cgccaacgca ggcaaatccg gcggcgaatt
    38401 tttcaccccg caaagcgtct ccaagctgat tgcgcggctg gcggtgcacg ggcaggagaa
    38461 agtcaacaaa atctacgacc ccgcctgcgg ctcgggcagc ctgctcttgc aggcgaaaaa
    38521 acagtttgac gaacacatca tcgaagaagg cttcttcggg caggaaatca accacaccac
    38581 ctacaacctc gcccgcatga atatgtttct gcacaacgtc aattacaaca aattccacat
    38641 cgaattgggc gacacgctga ccaaccccaa actcaaagac agcaaaccct ttgatgccgt
    38701 cgtctccaat ccgccctatt ccatcgactg gataggcagc gacgacccca ccttgatcaa
    38761 cgacgaccgc tttgcccccg caggcgtact cgcaccgaaa tccaaagccg attttgcctt
    38821 catcctgcac gcactgaact acctttccgg cagaggccgc gccgccatcg tctcattccc
    38881 cggcattttc tatcgcggcg gcgcagagca gaaaatccgc caatatctgg tggagggcaa
    38941 ctatgtggaa accgtgattg cccttgcgcc caatctcttt tacggcacct gcatcgccgt
    39001 caatatcctg gttttgtcca aacacaaaga caataccgac atccaattca tcgacgcaag
    39061 cggcttcttt aaaaaagaaa ccaacaacaa cgtcttaacc gaagaacaca ttgccgaaat
    39121 cgtcaaactc ttcgccgaca aagccgatgt gccgcatatc gcccaaaacg ccgcccagca
    39181 aaccgtcaaa gacaacggct acaacctcgc cgtcagcagc tatgtcgaag ccgaagacac
    39241 ccgcgaggtc atcgacatca gacagctcaa cgccgaaatc agcgaaaccg tcgccaaaat
    39301 cgaacggctg cggcgtgaaa ttgacgaagt gattgcagag attgaaacct aggcagttgt
    39361 tgcatatttt gcaacaactg aatgggaaag aggccgtctg aaaatacggg cggcaacctg
    39421 taagcaatcc ttacaagttc aaatcaagaa attttcgggg aaaatacatg acaacagaaa
    39481 ataacgcttt tgaaaacgcc aaacacatcg acgaaacagg caatgaatat tggtcggcgc
    39541 gcaccttgca gcaaatcttg gaatattccg aatggcgcaa tttccaacgg gccattgata
    39601 aagccataac cgcttgcgaa acatcaggaa atgacaaaaa tcatcatttt gttgaaacca
    39661 acaaaatgat agcccttggg aaaggcgggc agcgcgaagt ggcggattac cgcctttccc
    39721 gctatgcctg ctacctgatt gtgcaaaacg gcgatccgtc caaaagcgtc attgccgcag
    39781 ggcaaaccta ctttgccgtg caagcccgcc gccaagagct gcaagacgag gcagcattca
    39841 ggagtttggg cgaagacaaa caacgcctgc tgcttcgcag gcagctgcgc gaacacaata
    39901 ccgaccttgc cgccgcagcc aaggatgcag gcgtagaaaa acccgttgag tacgccgtct
    39961 tccaaaacca cggctatcgg gggctatacg gaggactgga caagcagggc atacacagcc
    40021 gcaaaggctt gaagaaaagc cagagaatcc tagaccacat gaacgccagc gaaccggctg
    40081 ccaatctgtt tcgcgccacg caaaccgaag aaaaactacg ccgcaaaaac atccaaggca
    40141 aaacacaggc aaaccgggtg catttcgaag tcggacaaaa agtgcgccaa accattgaag
    40201 aactgggcgg catcatgcct gaaaaccagc ccgtacccga gaaaagcatc aaacagcttg
    40261 agaacgaaga acagaaaagg cttgccgcaa ccgaacagca tcaaaacggc aaaaaataac
    40321 cgcattcagg ctacctgaaa aagcagcctg cacacgattt caggcagcct gaaagcagga
    40381 cgggcttcag cccgcagaaa taacggcaaa cggacatagc gggctgaaac ccgccccgca
    40441 accgccccat atcccgccgc agcgggaagg aaacggaaaa caaccatgga tatgcaaagc
    40501 aaagcgaaaa aattgattga gatgattcag acggcaccgg tggagtggaa gccattgggg
    40561 gaggttttag ttcgtacaaa aggaactaag ataactgctg gacaaatgaa agaaatgcat
    40621 aaagataatg cgccgttaaa gatttttgca ggaggaaaaa cttttgcatt agttgacttt
    40681 gatgatgttc ctgataagga tatccataga gagccttcta ttatcgttaa atcaagaggt
    40741 attattgaat tcgaatatta cgataaacca ttttcgcata aaaatgaaat gtggtcttat
    40801 cattcagtta ataaacatat ttatataaaa tatgtttatt atttcttaaa aacacaagaa
    40861 aactattttc gtaatatcgg atcaaaaatg caaatgccac aaatagcaac accagataca
    40921 gataactata aaatccccat cccatcattg gaaacccaac aaaaaattgt aaaaatactt
    40981 gacaaattca ccg
//
LOCUS       DAXFHF010000010        40525 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXFHF010000010 DAXFHF010000000
VERSION     DAXFHF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40525
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.010"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..106
                     /locus_tag="ACFIRM_000458"
     CDS             <1..106
                     /locus_tag="ACFIRM_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3028160.1"
                     /translation="DRKPFPPFLRECEFRFNFGTPSRQLKILRDRCGI"
     gene            281..2525
                     /locus_tag="ACFIRM_000459"
                     /pseudo
     CDS             281..2525
                     /locus_tag="ACFIRM_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2650..4200
                     /locus_tag="ACFIRM_000460"
     CDS             2650..4200
                     /locus_tag="ACFIRM_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC3028161.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4624..6408)
                     /gene="lpdA"
                     /locus_tag="ACFIRM_000461"
     CDS             complement(4624..6408)
                     /gene="lpdA"
                     /locus_tag="ACFIRM_000461"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3028162.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGKKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6426..6860)
                     /locus_tag="ACFIRM_000462"
     CDS             complement(6426..6860)
                     /locus_tag="ACFIRM_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028163.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6961..8550)
                     /gene="aceF"
                     /locus_tag="ACFIRM_000463"
     CDS             complement(6961..8550)
                     /gene="aceF"
                     /locus_tag="ACFIRM_000463"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC3028164.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSAAPAAVPTSASPAAAK
                     IDEAAFAKAHAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKP
                     AAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEE
                     ADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNY
                     FNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTIS
                     SLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGA
                     AGMRFTVFLANLLKDFRRITL"
     gene            complement(8700..11363)
                     /gene="aceE"
                     /locus_tag="ACFIRM_000464"
     CDS             complement(8700..11363)
                     /gene="aceE"
                     /locus_tag="ACFIRM_000464"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC3028165.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            12061..13773
                     /locus_tag="ACFIRM_000465"
     CDS             12061..13773
                     /locus_tag="ACFIRM_000465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC3028166.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13836..14492
                     /locus_tag="ACFIRM_000466"
     CDS             13836..14492
                     /locus_tag="ACFIRM_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC3028167.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14502..14957)
                     /gene="ruvX"
                     /locus_tag="ACFIRM_000467"
     CDS             complement(14502..14957)
                     /gene="ruvX"
                     /locus_tag="ACFIRM_000467"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC3028168.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14950..15498)
                     /locus_tag="ACFIRM_000468"
     CDS             complement(14950..15498)
                     /locus_tag="ACFIRM_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC3028169.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15500..16060)
                     /locus_tag="ACFIRM_000469"
     CDS             complement(15500..16060)
                     /locus_tag="ACFIRM_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC3028170.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGASFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16386..18182
                     /locus_tag="ACFIRM_000470"
     CDS             16386..18182
                     /locus_tag="ACFIRM_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3028171.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKHTEEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKA
                     QISRFVSGNYKNSRPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18309..19784
                     /locus_tag="ACFIRM_000471"
     CDS             18309..19784
                     /locus_tag="ACFIRM_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC3028172.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            20114..22141
                     /gene="uvrB"
                     /locus_tag="ACFIRM_000472"
     CDS             20114..22141
                     /gene="uvrB"
                     /locus_tag="ACFIRM_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC3028173.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(22319..23077)
                     /gene="cah"
                     /locus_tag="ACFIRM_000473"
     CDS             complement(22319..23077)
                     /gene="cah"
                     /locus_tag="ACFIRM_000473"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC3028174.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(23486..23833)
                     /locus_tag="ACFIRM_000474"
     CDS             complement(23486..23833)
                     /locus_tag="ACFIRM_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028175.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(24157..24873)
                     /gene="trmB"
                     /locus_tag="ACFIRM_000475"
     CDS             complement(24157..24873)
                     /gene="trmB"
                     /locus_tag="ACFIRM_000475"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC3028176.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDNLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(24995..26400)
                     /locus_tag="ACFIRM_000476"
                     /pseudo
     CDS             complement(24995..26400)
                     /locus_tag="ACFIRM_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(26517..28403)
                     /gene="uvrC"
                     /locus_tag="ACFIRM_000477"
     CDS             complement(26517..28403)
                     /gene="uvrC"
                     /locus_tag="ACFIRM_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC3028177.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGTERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(28458..30635)
                     /locus_tag="ACFIRM_000478"
     CDS             complement(28458..30635)
                     /locus_tag="ACFIRM_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3028178.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            30774..31724
                     /gene="trxB"
                     /locus_tag="ACFIRM_000479"
     CDS             30774..31724
                     /gene="trxB"
                     /locus_tag="ACFIRM_000479"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC3028179.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            31880..32248
                     /gene="rpsF"
                     /locus_tag="ACFIRM_000480"
     CDS             31880..32248
                     /gene="rpsF"
                     /locus_tag="ACFIRM_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC3028180.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLAAAAEEAAAQ"
     gene            32249..32551
                     /gene="priB"
                     /locus_tag="ACFIRM_000481"
     CDS             32249..32551
                     /gene="priB"
                     /locus_tag="ACFIRM_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC3028181.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGDCATVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            32558..32788
                     /gene="rpsR"
                     /locus_tag="ACFIRM_000482"
     CDS             32558..32788
                     /gene="rpsR"
                     /locus_tag="ACFIRM_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC3028182.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            32805..33257
                     /gene="rplI"
                     /locus_tag="ACFIRM_000483"
     CDS             32805..33257
                     /gene="rplI"
                     /locus_tag="ACFIRM_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC3028183.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(33880..34686)
                     /locus_tag="ACFIRM_000484"
     CDS             complement(33880..34686)
                     /locus_tag="ACFIRM_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3028184.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMLGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(34687..35433)
                     /gene="pssA"
                     /locus_tag="ACFIRM_000485"
     CDS             complement(34687..35433)
                     /gene="pssA"
                     /locus_tag="ACFIRM_000485"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC3028185.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            36017..36499
                     /locus_tag="ACFIRM_000486"
     CDS             36017..36499
                     /locus_tag="ACFIRM_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC3028186.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(36546..37757)
                     /locus_tag="ACFIRM_000487"
     CDS             complement(36546..37757)
                     /locus_tag="ACFIRM_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC3028187.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            37979..40417
                     /locus_tag="ACFIRM_000488"
     CDS             37979..40417
                     /locus_tag="ACFIRM_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3028188.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNEKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     FNGSRKILAHKDHL"
BASE COUNT         9802 a        10086 c        11041 g         9596 t
ORIGIN      
        1 cgatcgtaaa cctttcccgc cgttcttgag ggaatgcgaa tttcgattta acttcggcac
       61 accgtcccgg cagctaaaaa tcctgcggga tcggtgtgga atttagggat aatctagtac
      121 agccacaaaa ataaaccaat atttaacagt cggatagctc cgcctgatta agtttttcgg
      181 taatagttgt gattatcaaa tataattgcc gcccgaaata tccgataagt ttgtttgttt
      241 tatctttaat tccccaatac ttaaaaaaga gagcgttttc atgaacaaaa aacttgcctt
      301 aatgccgctg ttgattttga gcgcgttttc atctgccgcc gacaatactc cgcagcatgg
      361 cgaactcgga caggttcatg tccgcgccga tgccaagcgc gtcaaagccg cgcattctta
      421 ctccatcgcc agcgatggcg acttgcgcga ccgcgtaaat ttgggcgtat tgggtaaagc
      481 caacgcgttt accgcgccga ttaccgtcgt caactacgac gaacaagcct tcaacaatac
      541 cgaggcgcgt actttagtgg atgccgtagc caaaaaagac gcttccgttt gagagtttgg
      601 cggcgaaagc aacacgctga ccggtctgta tttccgtggt tatcagcttg atgcgcgcca
      661 attcagcgta aacggcttgg caggaatgta tggcacgcaa ggcacggcga gcgtgcaagt
      721 cggcttcgca caactgatta aaggcgcgtc caccgccgta aacggcatac cctgaaggcg
      781 cggtatccgg ttccgtcaac atcgaaacca aaaaagccgc cgacgaaggc aaccgcaaaa
      841 tcggtttggc ccggttcggc aacaaccgcg cccaaggtag gttcgactta gggcaacgct
      901 tcggcgaaaa caaagcattc ggcgtgcgtg ccaacggcaa actgcgccac ggcgacaccc
      961 cgcgccacgg ttacagagaa gacaacaaag aatttgcctt aaatgccgat tatcgcggcg
     1021 aaaaactgcg cgtgacgttc gactccatct acgcgaaacg caaaatcaac ggcggccgcg
     1081 cgcgtatgca ggatatccaa aacgccggcg gacgcttgtt cgacgcgccc gacggcaaaa
     1141 tcaacctgct gccttcttgg aactggcaaa ataccgtcgg cgaaaccaat atgctgacgt
     1201 ttgaatggga tgcgtttgac aatacctaaa ttaccggcgg tatcggctac aacaaagcgc
     1261 gttattacgg tacgctgatt tccccaaccg tttgcggtac aagcggtgcg agcagccaaa
     1321 ccaaaacctg catggctgcc aaccaatacc acaccggcac ggcacgcctg accgaccaat
     1381 atttccgtac tttgagtatg aacctgaccg cacgcggcga atttgaaacc ggcccggtca
     1441 cgcataactg gagtacggct ttcgaccgcg ttatccgcca gcggaaaacc ataaacggca
     1501 gcgggaacgg caacagcaag atagaagtga aggcaaacga aaatattgcg aatcaattgg
     1561 catcgttcaa agccgactat cccaattctt gggcgaactc ggccaacttg gatgccaata
     1621 tcaaagtcaa cagcttggct ttgtccgata ctttgggctt cgttgataac aaataccccc
     1681 tgactttggg cggacgtttc caagcggttg aatacactga taagaagaaa tcacagagcg
     1741 gcgatgccaa acgtttcagc ccgatgctga tggcggcgtg ggtgccgcaa cctgatttgg
     1801 tcgtgtgcgg caactatatg gaagacttgg agcgtgccga tatcaaaacc gatgattccg
     1861 gcgaaacgac aatggcgaag ccgcgcgtca gccgccagtt tgaaatcggc gtgcgtaaaa
     1921 actggggcga ttttgtaacc acgttgaacg cgttccaaat caaacgcccg ggctactggc
     1981 gcggcaatac caaaaaaggt actgattttg cagcatataa agcagcagga ggcgcagccg
     2041 gcgacgagca aggtatggaa cgcaatcgcg gtattgagtt caatgcctat gccaacctgt
     2101 tgaacaaaac cctgcgcccg actttcggcg tgatgtatct gcaatcgtcc gtgaagaatt
     2161 atccgaatgc gcgcgatatg ctggttaacg gcgtacaagt cgccaacccg cgcgtgattg
     2221 tcaaaacagg cgttgaacgg gatacaccgt ttgccaaagg tttgagcttg aacggcaatg
     2281 tttcgtattt cggcaagtct tatcaagaca cgcaaaaaca atacgccttc ccgtcctata
     2341 ccttggttga cgtaggcgcg cgctacaaaa ccaagctggg caaaaacacc ctgaccgtca
     2401 gcagctcggt agaaaacctg ttcaacaaaa actactggca ggtacagcgc ggacaatttg
     2461 accgtagttt cgccgtcgtc ggtctgccgc gtacttactg gctgaaggcg gaattggatt
     2521 tctaagctgc ctccgtttcc gaaaatgccg tccgaacagg tttcagacgg catttctttt
     2581 aaagaaatat ggcgggatat gcttaaataa tgctattcta gtacctttga aacctgcttg
     2641 tttgaagtga tgaaaaaacc tttgatttca gttgcggcag tattgctcgg cgttgctttg
     2701 ggtacacctt attatttggg tgtcaaagca gaagaaagtc tgacgcagca gcaaaaaata
     2761 ttgcagaaaa cgggcttttt gaccgtcgaa tcgcaccagt atgatcgagg ctggtttacc
     2821 tctacggaaa cgacggtcat ccgtctgaaa cccgagttgc tgcataatgc gcagaaatac
     2881 ctgccggata acttgaaaat agtgttggaa cagccggtta cgctggtaaa ccatatcacg
     2941 cacggccctt tcgccggcgg attcggcacg caggcgcaca ttgaaaccga gttcaaatac
     3001 gcgcctgaaa cggaaaaagt tttggaacgc ttttttggga aacaagttcc ggtttccctt
     3061 gccaataccg tttatttcaa cggcagcggt aaaatggaag tcagtgttcc cgctttcgat
     3121 tatgaagaac tgtcgggcat caggctgcac tgggaaggcc tgacggggga aacggtttat
     3181 caaaaaggtt tcaaaagcta ccgcaacagc tatgatgcgc ccttgttcaa aatcaagctg
     3241 gcagacaaag gcgatgccgc gtttgaaaaa gcgcatttcg attcggaaac ttcagacggc
     3301 atcaatccgc ttgctttggg cagcagcaat ctgactttgg aaaaattttc gctcgaatgg
     3361 aaagagggtg tcgattacaa cgtcaaattg aacgaactgg tcaacctcgt taccgatttg
     3421 cagatcggcg cgtttatcaa tcccaacggc agcatcgcac cttccaaaat cgaagtcggc
     3481 aagctggctt tttcaaccaa gaccggggaa tcgggcgcgt ttatcgacag cgaagggcgg
     3541 ttccgtttcg atacgttggt gtacggcgat gaaaaatacg gcccgctgga catccatatc
     3601 gctgccgaac acctcgatgc ttctgcctta accgtattga aacgcaagtt tgcacaaatt
     3661 tctgccaaaa aaatgactga ggaacaaatc cgcaatgatt tgattgcggc agtcaaaggc
     3721 gatgcttccg gattatttac ccatgacccg gtactaaata tcaaaatttt ccgtttcacc
     3781 ctgcctcagg gaaaaattga tgtgggcgga aaaatcatgt ttaaaggcat gaagaaggaa
     3841 gatttgaacc aattgggact gatgttaaag aaaaccgagg caaacatcag aatgagtatt
     3901 cctcaaaaaa tgttggaaga tttggcggta agtcaggctg gaaatatttt cagtgtaaat
     3961 gccgaagatg aggcggaagc cagagcaagc attgccgata ttaatgaaac attgcgcctg
     4021 atggtggaca gtacggtcca aagtatggca agggaaaaat atcttacttt agacggtaat
     4081 cagattgata cggtcatttc ccttaaaaac aacgccctga agttaaacgg gaaaacgctg
     4141 caaaatgaac ccgatcctga ttttgacgag ggagatatgg tttccggcca gccgcattaa
     4201 tgccgtttat gccaaatgcc gtctgaaatg ttcagacggc atttttgtcg gtctcgaaat
     4261 aggatggatt ttcatgtttc aaaaacaatt gattgtctct aatccgatat caggacacgc
     4321 ttccaatgtt tagatatgga attcggacgg gataggggaa ctattgaaat ccagttgaaa
     4381 tctgttattg gaaagcatgg aggaaaaata aaaggctgta ttttaatata gtattgatag
     4441 gcatgcttag cttcaaggta ttcaatagtg taggtgtctg cctaactgga aaataatcgt
     4501 cttttccctt gcaaccagtt aaaattgaca gatggttaaa acacagccaa ataaaaatgc
     4561 cgcctgaagg gttcagacgg catttgttcg gataaacctt atcggctgct tagagcatca
     4621 ggtttatttt ttcttttgag gaggcaggtc ggtacataca cccaaagcca cttccgcagc
     4681 catacctatg gattcgccca aggtcgggtg cgggtggatg gttttgccga tgtctgccgc
     4741 gtcgcagccc atttcgatgg caaggcagac ttcgccgatc atgtcgccac cgttcggacc
     4801 gacaatgccg ccgccgatga tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc
     4861 gttgtcgcaa ccgttggcaa tcgcacgacc ggaagccgcc catgggaagt tggctttggt
     4921 gattttgcgg cctgatgctt tggcagacaa ttcggtttca ccgacccatg ccacttcggg
     4981 ggaagtgtag gcaacgccgg gaataacgcg tgcgtcgaag taggctttgt ggccggcaca
     5041 gttttcagcg gcaacgtgac cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac
     5101 gatatcgccg atagcgtaga tgtgcggtac gttggtgcgc atttgtttgt caacttcgat
     5161 gaagccacga tcggtaacgg caacaccggc tttttctgcg ctgatgagtt tgccgttagg
     5221 cgcacggcct gccgcgacca gtacggcatc gtaacgttgc ggctctttag gtgcatttgc
     5281 accttcaaag gtaacgtaaa cgccgtcttc tttaggctcg acagcaacag ttttggtgtt
     5341 gaccataatg ttgtcaaaac ggtattcgtt ttgtttttgc catactttaa ccaagtcgcg
     5401 gtctgcgcct tgcatcaggc cgtccatcat ttcaaccaca tccaggcgag aacccagcgt
     5461 gctgtaaacc gtacccatct cgaggccgat aatgccgccg ccgataatca gcagtttgcc
     5521 cggtacttct ttcagtgcca gtgcgccgct ggaatcgatg atgcgcggat cttcaggaat
     5581 gaaaggcagt ttggttacgc ggctgcctgc tgcaatgata cagtttttga aggcaacgat
     5641 ttttttcttg ccggtagggg ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc
     5701 caagtggtga ggatccaaga attgcccgtc gccttggata acgtccactt tacggctttt
     5761 cgccatacct gccaaaccac ccgtgaggcg ggaaaccacg ccgtctttgt aggcgcgaag
     5821 catatcgatg tcgagttccg gttcggggta tttgataccg ttggcagcca agtggcgcac
     5881 ttcgtcgata acggcggcat tgtgcaacaa ggctttggaa gggatacagc cgacgttcag
     5941 gcaaacgccg cccaaagttt tgtaacgctc gacgatggcg actttcaagc cttcatcggc
     6001 agcggcaaat gcagcggagt aaccgccggg accgccgccc aatacgacca cgtcgtactc
     6061 ggcatcggca gcaccgccga attgcgcggc ttgcggagca ggagcggcag ctttgggtgc
     6121 ttcttgcgcc ggggcggcag ccgcttcggc tttaggggcg gcagccgcac cttcggcttc
     6181 gacgacgaca atcaaaccgc cttcggagat tttgtcgccg actttgactt tcacttcttt
     6241 gatcacgccc gcaacttcgg caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat
     6301 cagggtgtcg tcaacggcga tggtgtcgcc aacgttcact tcaaccgcga taatatctac
     6361 attttcgtgt ccgccaatgt cgggcacttt caattcaact aaagccatta tattgacctt
     6421 tcaacttagt taatttctat caactcttta gcaataatca cttcttcagg aatacagcca
     6481 caatcttcag ctctgattgt tacttccttc tcatctgggt tttctggatg ttcaacctca
     6541 tattcaaaaa tagaatattg accaaacaaa tctctattta aaacatatat atagccattt
     6601 tcgatgccgg agcttgttgc aaatttcttg gcaatttcct tgtctgttgt cgtagatata
     6661 taacatccgt catatagatc tgtttcaatt tgatgggcgt aaactgcatt cttcacactt
     6721 ggaccatgtg tagctttacc atcatattta aacttaccat cataacgaat tgcaacttca
     6781 gctttattac ctttaggttt taactgtcca ttattttgct catcttgttg gcaactaatg
     6841 cctctatata aataagaaat ttcccataag ttattaattt aaagtttgaa tttaacacca
     6901 tcactcatca atctcaagca atccaaacat tatcagactg cttgagaggg atgttttatt
     6961 ttataaggta atgcggcgga agtctttcaa caggttcgcc aggaatacgg tgaagcgcat
     7021 accggcggca ccgtcgatga cgcggtggtc gaaggacagg ctcaacgggc acatcaggcg
     7081 cggggcaaac tctttgccgt tccaaaccgg tttgatttgg gatttgcaca cgcccaagat
     7141 agcgacttcg ggagcgttca caatcggcgt gaaacctgtg ccgccgatgc cgcccaagct
     7201 ggaaatggta aagcacgcgc cttgcatttc ttgcggtttg agcttgcctt cgcgggcttt
     7261 tttggacaat tcggtcaatt cttggctgat ttgtttcaag cctttttgat ccacgtcttt
     7321 gatgacggga acaaccaagc cgttcggcgt atcggctgcg aaaccgatgt tgaagtagtt
     7381 tttcagcacc aggttgtcgc cgtccaaaga agcgttgaat tcggggaagg ctttcagcgc
     7441 ggaaacggag gctttgatga tgaacgccaa cggggacagt ttcacgcctt cgcgttccca
     7501 ttctttgttc agctgtttgc ggaattcttc cagttcggtc atatccgctt cttcgtgtac
     7561 ggtaacgtga ggaatcacca cccagttgcg ggacaggttt tgaccggaga ttttcttaat
     7621 gcgggacaat tctttaactt cgacattgcc gaatttggag aagtccactt taggccacgg
     7681 cagcaagtcc agaccgccgc ccaaagatgc gccggctgcg gcaggttttg ccgcgccgcc
     7741 ctgcattacg gatttcacaa aggctttgat gtcgtctccc atgatacggc ctttcaagcc
     7801 gctacctttg acttggccca aatccacgcc caattcgcgc gccagtttgc gtgcggaagg
     7861 accggcgtgt gctttggcga aagcggcttc atcgattttg gcagcggcag gcgaggcaga
     7921 tgtaggcacg gcggcgggtg cggcagatgt aggcacggcg gcgggtgcgg cagcttgagc
     7981 ggcaggcgca ggagctgctg cggcagagcc ggcggtttcc acttcgataa tggcagagcc
     8041 ttcggacact ttgtcgccga cttttaagaa tacggcttta acgacaccgg cagcggtaca
     8101 aggtacgtcc attgtcgctt tatcggtttc caaagtaatc agcgtgtcgt cttcggcaac
     8161 ggtgtcgcca actttgattt caacggcgat tacatctaca tcggtatggc cgccgatatc
     8221 gggaacggct acttgaacgg ttgcaccgcc tgcggcagcg ggtgcaggtt gtgcttcggc
     8281 ggcagcagcc ggggcggctt cggcagcggc ggcaccggtt tcaacggtca aaattacgcc
     8341 gccttcggag attttgtcgc cgactttgac ttttacttct ttcacgacac cggccgcatc
     8401 ggcaggcaca tccatcgtgg ctttgtcggt ttccagtgta atcagggtgt cgtcaacggc
     8461 gatggtgtcg cccgctttaa cttctacggc gatgatgtcg acgctttcgt gaccgccgat
     8521 atcggggact ttgatttcta cgatactcat ttgagttcct ttaatctttt tcagttggat
     8581 gccgtctgaa agctgcttca ggcggcatcg gtacggtttg tctgttcaaa ccattcgggc
     8641 attcggtttt cagacggcct aatgttggca tgaaacaaac aggccgtctg aaacatcaat
     8701 cagcgtttcc agctaggggc tgaatcggct ttgatgccgt atttctcaat ggcttgttga
     8761 acggtttctt tgctgacttt gccttgttcc gccaatgcgg ccaatgctgc cacggcaacg
     8821 ttgtagcggt ccacttcaaa gaagcggcgc aggttggcgc ggctgtcgga acggccgaaa
     8881 ccgtcagtgc ccaagacatg gtagtcgttc gggatgtacg cgcggatacg gtcggcatag
     8941 ctgcggatat agtcggtagc ggcgataacc ggaccgtcat gaccttgcag ttgggaagta
     9001 acgaaaggca ctttttcagc ttccagcgga tgcaggcggt tgaagcgttc ggtttcgatg
     9061 gcgtcgcggt gcagcaggtt gaaggacggg caagaccaga tgtccgcttc cacgccgaag
     9121 tcggctttca gcagctcggc accggcaatc acttcttgca gaatcgtacc ggaacccatc
     9181 aattggactt tcttgtcgcc tttgccgccg gctttcagca ggtacatacc tttgaggatg
     9241 tcttgttccg caccttccgg catatcggga tgggtgtagt tctcgttcat cagggtgatg
     9301 tagtagaaca cgtcttcatt attggcatac atacggcgca gaccgtcttg tacgatgacg
     9361 gccacttcgt attggaaggt cgggtcataa gatacgcagt tcggaatcag gtcggcctgg
     9421 atgtggctgt ggccgtcttc gtgttgcaga ccttcgccgt tcagcgtcgt acggccggca
     9481 gtaccgccca gcaggaagcc gcgcgcgtgc atatcgcccg ccgcccaagc caagtcgccg
     9541 atacgttgga aaccgaacat ggagtagtag atgtagaacg gaatcatcgc gaagtcgctg
     9601 ttggcgtagc tggtcgcagc cgcaatccag tcggccatcg cgcccggttc gttaatacct
     9661 tcttgcaaga tttgaccgtc gacggattct ttgtagaaca tcagttggtc tttatcttga
     9721 ggcgtgtatt gttggccttt gggattccaa ataccgtatt ggcggaacat accttccatg
     9781 ccgaaagtac ggctttcgtc gggaacgata ggtacgacgc gtttgccgat ttttttgtct
     9841 ttcagcaaag tggacaggat gcggacgaat gccatcgtgg tcgagaactc gcgttcaccg
     9901 ctggatttga gttgtgcgtc gaatgccgac agctcgggca cttccaatac ttcctgcgtc
     9961 ggtttgcgtt gcggcaggta gccgcccaaa gcatcgcggc gtgcgtgcag gtatttgtat
    10021 tcttccgtat cgggggcaaa agtcaggtaa ggcagatcgc cgctttcgat ttgttcgtcg
    10081 gtaaccggaa tgtcaaagcg gtcgcggaat tgtttcaggg acgctttgtc catttttttg
    10141 gcttggtggg caacgttctg accttcgccg gatgcgccca taccgtaacc tttaatggtt
    10201 ttcgccaaga taacggtagg tttgccgtcg gcatggttcg ctgcgcggtc gtaggcgttg
    10261 tacacttttt gaggatcgtg gccgccgcgg ttcaatgccc agagttgctc atcggtcata
    10321 tcggcaacca atgctttcag ttcgggcgta ttgaagaagt gttcgcgcac atacgcgccg
    10381 tctttggatt tgtaagtttg gtagtcgccg tccaaacatt cttccatacg ttggcgcagg
    10441 ataccgtctt tgtctttcgc caagaggcgg tcccaacggc ggccccaaat gactttgacg
    10501 acattccagc cggcaccggc aaagttgcct tccaattctt ggatgatttt gccgttgccg
    10561 cgcaccggac cgtccaagcg ttgcaggttg cagttgatga cgaaaatcag gttgtccaag
    10621 ccttcgcgtg cagccagcgc gattgcacct tgggattcgg gttcgtccat ttcgccgtcg
    10681 ccgcagaaac accatacttt acggcctttg gtttttgcca agccgcgtga ttccaagtat
    10741 ttcaggaaac gcgcctgata aatcgccatg atggggccca agcccatgga tacggtcggg
    10801 aactgccaga agtcgggcaa gaggtggggg tgtggatagg aaggcagacc gtgtccgtcc
    10861 acttcttggc ggaagttgtt cagctggtct tcggtcagac ggccttcgac gaatgcgcgt
    10921 gcatagatgc ccggggcaac gtgaccttgg aagaagacca aatcgccttc ttcaccttca
    10981 cctttggctt tccagaagtg gttgaaaccg acttcgtaca tggtggcggc agattggaaa
    11041 gatgcgatgt gcccacccag ttccaaatct ttcttgccgg cgcgcaatac gatggcggcg
    11101 gcgttccagc gtacgaatgc gcgaatgcgg tgttcgatgt tttggtcgcc cggaatgcct
    11161 ttttcgtttt caaccgaaac ggtattcaaa tacggggtgg tcgtgccgtg gggcatacgt
    11221 acgcctttgt cgcggcagta tttgagcagg ttttccaaga ggtattgcgc gcgttcgccg
    11281 ccttcatatt cgaggacgga gcttaacgcg tccagccact cttgggtttc gatagggtca
    11341 acatcgtgta attgggtgga catagtatct atcctttatg ttgagtgttt aatcggaagg
    11401 gagggtgtag ccaacccctt tattttcgtt tgtgaaaaat aataagcaaa aacgaaaatt
    11461 tctaaatcaa accgaaacaa aataatttaa gtttaatgtt ttcaaaaaat tataaaagtc
    11521 ttggaagcaa gggaaaactt gaaaaaaaaa tgttcggtct gctccgcacg aaatcctatc
    11581 agcaatcggg tctgctggca aataaccatg cttttaaaag caggttttat actgaagttg
    11641 gcgtttggta aggacaggtc tttttgattg cgaaaacatt ctgtttaatt ttggttatat
    11701 tttttcggaa ttgtgggata tcgaatcgtt atgtctctgt cggcaatttt cctattgcag
    11761 aaaatctata cacaagcttt gtggacaacc gtgttgataa gccacggctt gtgttaaaaa
    11821 agtcttacgc aacaaatgta cgcttatttt gattaaaaat taggcaaatt taaatctata
    11881 aaaatcatgt aattatgttc aatatgatgt ttctggtcat gtttttgccg tctgttatgg
    11941 ggtttgtccc gcgtttgggg cagggggtga attttggagg ttggtgcaaa acctgctttt
    12001 ttcgcgttac aatctgcacc cgatttactt ttgaatctgc ctgtttggaa aaatactttt
    12061 atgaaagcca gccaattctt tatctctact ttaaaagaag cccctgccga agccgcgttt
    12121 gccagccaca agctgatgat tcgcgccggt ctgattaaag ccaacgcgtc cggtctttat
    12181 acttggatgc cgatggggct gcgcgtgtta cgcaaagtcg aaaacgtcgt gcgcgaggaa
    12241 atggcgcgcg cgggcagcgt ggagctgctg atgccggtgg tgcagcctgc cgaactgtgg
    12301 caggaatccg gccgctggga gttttacggt aaagaactgc tgcgcctgaa agaccgccac
    12361 gaacgcgatt tctgcatggg cccgacctgc gaggaagtca tcgccgacat cgtgcgcaaa
    12421 gaaatcaaca gctacaaaca actgccgaaa aatttttacc acatccaaac caaattccgc
    12481 gacgaagtgc gcccgcgttt cggcgtgatg cgcgcgcgcg aatttgtgat gaaagatgct
    12541 tattccttcc acgccgacta cgcctcgctt caggcgacct atgatgccat gtatgacgct
    12601 tactgccgca tctttacccg tctgggtttg gcgttccgcc ccgtcgccgc agacaccggc
    12661 agcatcggcg gtacgggttc gcacgagttt caagtgttgg cggaaagcgg cgaagatgtg
    12721 attgcataca gcgacacttc cgattacgcc gccaatatcg agttggcacc gaccttgccg
    12781 cttaaaggtg aacgtgccgc cgctcaggct gtgttgacca aagtacatac accaaacgtc
    12841 aaaaccattg agtctttggt tgaattcctg aatattccgg ttgaacaaac cctcaaatcc
    12901 atcgtggttg aaggcgaaaa cgaaggcgaa ctcgtcctac tgctgttgcg tggcgaccat
    12961 gagtttaacg acatcaaggc agaaaaactg gcgggcgtaa aatcgccact gactatggca
    13021 agccctgccg cgattgttga acaattcggc gcaaacggcg gttcgctcgg ccccgtcggc
    13081 ttcacaggca aagtctatgc cgatttcgct accgaaaaag gcgcggactg ggttatcggc
    13141 gcaaacgaag acgactacca ctataccggc ttcaacttcg gccgcgatgc cgccgagcct
    13201 gaattcgtcg atttgcgtaa tgtggtcgaa ggcgacgaaa gccccgacgg gcaaggccgt
    13261 ctgaaactgg cgcgcggcat cgaagtcgga catgtcttcc agttgcgcgg caaatatacc
    13321 caagctatga acgtcagctt cctcgacaat aacggcaaat cgcaaatcat ggaaatgggc
    13381 tgctacggca tcggcatcac ccgcgtcgtt gccgccgcca tcgagcagaa taacgacgaa
    13441 aaaggcatca tttggaccaa agcgatggcg ccgtttgaag tcgtcatcgt gccgatgaac
    13501 tacaaaaaat ccgacactgt gcgtgaagcc gccgacagaa tctatgccga attgctggcg
    13561 gcaggcgcgg atgtgctgct ggacgaccgc gacgaacgcg caggcgtatt gctgaacgat
    13621 tccgagcttc tcggtatccc gcaccgcatc gtcatcggcg accgcgcctt gaaagaaggc
    13681 aatgtcgaat acgccgaacg ccgcgacaac gaagcgcagg cagttgcaat cggagaaatt
    13741 gttgcgcgtg taacagcttc attaaatgcg taagtctggt caaatgccgt ctgaaaataa
    13801 tttcagacgg catttttatt ttgggggaaa gtgaagtggc ttcagtattt ttagaaggag
    13861 aagcagtcga ggtcggcaat attttctgta tcggcaggaa ctatgccgca catgtcgaag
    13921 agttaaaaaa cgaaatacct tcagaacctg tggtgtttat gaagccgtca ggcagcattt
    13981 tgaacagcgg cggtacaatc ttgctgcccg agttcagtag ggatgtgcag tttgagtgcg
    14041 aactagtgtt gctgattggc cgggattcag acggcacggg cgagggtgaa gatattttgg
    14101 gatgcgtggc ggggtacggc gtgggtttgg atttgaccgc gcgggacatc caatgccgtc
    14161 tgaaggcgca ggggctgccc tggctgaagg caaaagggtt caggcattct gcgtgcgtgt
    14221 cggattttgc ggcggcagac agaatcggca atcaagataa ggtgttgttt tcattaaaac
    14281 aaaacggcgt attgaaacag cgcggggaga ctggtttgat gatttacccg attcgggaaa
    14341 ttttacacaa actggcggcg gattacggtt tggggaaagg ggatttggtg tttaccggaa
    14401 cgccctccgg cgtgggcgca atcggtgcgg gggacaattt ggcattggag ctggacggct
    14461 tggtgtgcgc ctctttcacg atagggtgtt aaccgcgccg cttaaccttc acgcccgttg
    14521 aaacattccg ccgggccgcc ctcgagaaaa ccgtgcagga tggcttgcgc cgccacttgg
    14581 tcgagcaccg atttgcgttt tttgcccaag acctgtgctt ccgaaagcag gctttcggca
    14641 tagacggacg acagccgttc gtcaacccaa tagacgggga gattgaacct gccgttcagc
    14701 ctgcgtccaa acttgcgcga caggtgcgtc atttcatgtt ccgtgccgtc ggcatgcacg
    14761 ggcagtccga cgacaaaata acgcggctgc cattcttgaa ccagcttggc gattgccgcg
    14821 aatttttcat cgttgctgcc gccggtaacg gtggccagcg gatgggacag ccccaattcc
    14881 gcatcgccct gcgccacgcc gatgcgcgct tcgccgaagt cgaatgccag tgccgttcct
    14941 tttggaattt tatgcatggc cggctcccga aaacagggcg agcgggtcga tgccgagttt
    15001 ggcgaatgcg gcggcgtaac ggtgttcgta ggggatgtcg aacaggatgt gttcgtcggc
    15061 gggaacggtc agccacgcat tgtcggcaag ttcgcgttcg agctgccctt tgctccagct
    15121 cgaatagcct atgctgatca aggctttgtc aaccgcgcct tcgcgtgaaa tgttttcaag
    15181 cacatcgcgg gaagaggtca gcgcgatgcc gtctgaaacg ccgatgctgc tttgccagtt
    15241 gccgatcgga gtgtggacga cgtaaccgcg ctcgacctgc accggcccgc ccatcatcac
    15301 gctgtcgtgc tgcatccgca tggggatgtt tttgccggtg gcggaaaaaa tcatgtccat
    15361 cgtaatcgga gagggtttgt tgatggcgat accgagtgcg ccgtcttcat cgtgtttgca
    15421 gatatagacg accgattgtg aaaaaaacgc gtcttccata tcgggcatgg cgacgagaaa
    15481 gtggttggac aggttcatat cagcgtttgt cgggattttt tatcgggttg gaaataatgc
    15541 aggcgccggc aagcagtttg ccggaagtat cggcttgcac cccgtcggtt tgcggtatgc
    15601 cgctgccgaa acaggaaacc gcgcttaagg aatttttcgg cgatccttgg acgattttat
    15661 cgctgtaaac caaataggcg aaggctttgc gcttggggtc gtaataacgg acaatctgcc
    15721 ggctcttgaa cgcgaagctc gcgccgcgct tgaaaacttc tttcggtttg cgcacggcgg
    15781 tttcgtcaaa agaaatcgaa gatgccgtct gaacgcacga aaccgatgcg tcggacgcgt
    15841 cctcttccaa attgaccatt tccttcaagc cgcctttttt tgcatacgaa atataacagg
    15901 caaccccttg aacgtcggga tcgtcgaatc cttccacttc gatacggtcg tttttgccca
    15961 acatgttgaa aacggtactt gcccgtccga ttttatcggt ttcgccgctg ccgcaggcag
    16021 tcggcaggac ggcggcagac agcagtagca gtctgttcat caaaatttcc ttatcggttg
    16081 aatccccgtt ttccggaagg cggcagggtt tgctttgttt ggaggggcgc acgctcccaa
    16141 atcgggcaac atacggtacg cagcttacat gtgctaccct gtgtgtcgga acacaatatc
    16201 cgttgcagca ttgaaaatat gccgcacaat ataaggctgt ttgagccgat tgcaagtatc
    16261 cgcgcaaaga aggtgcaaga ttgggcaaac ggcattttca gacggcataa ctgacagtat
    16321 aatccaaacg tccggctgct ttcgtgcagc cttcagtatt atccaacttt tccgaaggtc
    16381 gaaccatgcg ctacaaaccc cttctgcttg ccctgatgct cgttttttcc acgcccgccg
    16441 ttgccgccca cgacgcggca cacaaccgtt ccgccgaagt gaaaaaacag gcgaagaaca
    16501 aaaaagaaca gcccgaagcg gcggaaggca aaaaagaaaa aggcaaaaat gccgcagtga
    16561 aagataaaaa aacaggcggc aaagaggcgg caaaagagtt caaaaaaacc gccaaaaacc
    16621 gcaaagaagc agagaaggag gcgacatcca ggcagtctgc gcgcaaagga cgcgaagggg
    16681 ataaggaatc gaaggcggaa cacaaaaagg cacatggcaa gcccgtgtcc ggatccaaag
    16741 aaaaaaacgc aaaaacacag cctgaaaaca aacaaggcaa aaaaggggca aaaggacagg
    16801 gcaatccgcg caagggcggc aaggcggaaa aagacactgt ttctgcaaat aaaaaagccc
    16861 gttccgacaa gaacggcaaa gcagtgaaac aggacaaaaa acacacggaa gagaaaaatg
    16921 ccaaaaccga ttccgacgaa ttgaaagccg ccgttgccgc tgccaccaat gatgtcgaaa
    16981 acaaaaaagc cctgctcaaa caaagcgaag gaatgctgct tcatgtcagc aattccctca
    17041 aacagcttca ggaagagcgt atccgccaag aacgtatccg ccaagagcgt atccgtcagg
    17101 cgcgcggcaa ccttgcttcc gtcaaccgca aacagcgcga ggcttgggac aaattccaaa
    17161 aactcaatac cgagctgaac cgtttgaaaa cggaagtcgc cgctacgaaa gcgcagattt
    17221 cccgtttcgt atcggggaac tataaaaaca gccggccgaa tgcggttgcc ctgttcctga
    17281 aaaacgccga accgggtcag aaaaaccgct ttttgcgtta tacgcgttat gtaaacgctt
    17341 ccaatcggga agttgtcaag gatttggaaa aacagcagaa ggctttggcg gtacaagagc
    17401 agaaaatcaa caatgagctt gcccgtttga agaaaattca ggcaaacgtg caatccctgc
    17461 tgaaaaaaca gggtgtaacc gatgcggcgg aacagacgga aagccgcaga cagaatgcca
    17521 aaatctccaa agatgcccga aaactgctgg aacagaaagg gaacgagcag cagctgaaca
    17581 agctcttgag caatttggag aagaaaaagg ccgaacaccg cattcaggat gcggaagcaa
    17641 aaagaaaatt ggctgaagcc agactggcgg cagccgaaaa agccagaaaa gaagcggcgc
    17701 agcagaaggc tgaagcgcga cgtgcggaaa tgtccaacct gaccgccgaa gacaggaaca
    17761 tccaagcgcc ttcggttatg ggtatcggca gtgccgacgg tttcagccgc atgcagggac
    17821 gtttgaaaaa accggttgac ggtgtgccga ccggactttt cgggcagaac cggagcggcg
    17881 gcgatgtttg gaaaggcgtg ttctattcca ctgcgcctgc aacggttgaa agcattgcgc
    17941 cgggaacggt aagctatgcg gacgagttgg acggctacgg caaagtggtc gtgatcgatc
    18001 acggcgagaa ctacatcagc atctatgccg gtttgagcga aatttccgcc ggcaagggtt
    18061 atacggtcgc ggcaggaagc aaaatcggca cgagcgggtc gctgccggac ggggaagagg
    18121 ggctttacct gcaaatacgt tatcaaggtc aggtgttgaa cccttcgggc tggatacgtt
    18181 gatcggattg ccgaaacggc aggctttatg caaatcgtgc cgatatggtg cgctgttctg
    18241 tgaagtttgc cgtttgccgt acctgtgttt tccggttttt ggaaatcaag agagaaagtt
    18301 aaagaagaat gtcgaaacct gtttttaaga aaatcgcact ttatactttg ggtgcaatca
    18361 gcggcgtggc cgtaagtctg gcggtgcagg gttttgccgc cgagaaggac gggcgggata
    18421 acgaagtcct gccggtgcaa tccatccgta cgatggcgga ggtttacggt cagattaagg
    18481 caaactacta tcatgacaaa cccgatgccg atttgtttga aggtgcgatg aagggtatgg
    18541 tggccggttt ggatccgcat tccgaatata tggataaaaa aggttatgcc gagataaagg
    18601 agtccaccag cggcgaattt ggcggcttgg ggatggaaat cgggcaggaa gacggttttg
    18661 tcaaagtggt ttcgccgatt gaggacacgc ctgccgaacg ggcggaggtg aaaagcggcg
    18721 atttcattgt gaaaatcgat aatgtttcga cgcgcggtat gacggtcagc gaagcggtga
    18781 aaaaaatgcg gggcaagccg ggtacgaaga ttactttgac gctgtcgcgc aaaaatgccg
    18841 acaagccgat agtcgtcaac ctgacccgtg ccattattaa agtgaaaagc gtccgccatc
    18901 acctgatcga acccgattac ggctatatcc gcgtgtcgca gttccaagag cggacggtcg
    18961 aaagcgtcaa taccgccgca aaagagctgg taaaggaaaa taaaggaaaa ccgctcaagg
    19021 ggctggtgtt ggatttgcgc gacgaccccg gcgggctttt gaccggcgcg gtcggcgtgt
    19081 cggcggcgtt tctgccgtct gaagcggtcg tcgtcagcac caagggacgc gacggcaaag
    19141 acggcatggt actgaaagcc gttcccgagg attatgtgta cggtatgggc ggcgatcctt
    19201 tggcgggtat tcctgccgag ttgaaaacga ttccgatgac ggtattggtc aattccggtt
    19261 cggcttccgc gtcggagatt gtcgccggcg cattgcagga ccacaaacgc gcggtcatcg
    19321 tcggtacgca gagcttcggt aaaggttcgg ttcagacttt gattcctttg tccaacggca
    19381 gcgcggtcaa gttgacgacc gccctgtatt acacgccgaa cgaccgttcc attcaggcac
    19441 aggggattgt tcccgatgtc gaagtaaaag ataaggaacg tacttttgaa agccgcgagg
    19501 cggacctggt cggacacatc ggcaatccct tgggcggcga ggatgtgaac agtgaaaccc
    19561 ttgccgtacc gcttgaaaaa gatgcggata agcccgctgc aaaagaaaaa ggtaaaaaga
    19621 aaaaggacga ggatttgtct tcaaggcgga ttccgaaccc tgcgaaagac gatcagttgc
    19681 gtaaggcttt ggatttggtc aagtcgcccg agcagtggca gaagtctttg gggctggcgg
    19741 cgaaaaagcc ggtttcaaat aaagataaaa aagataagaa gtaggttttg acgatgccgt
    19801 ctgaaagggt ttcagacggc atattatgtt tgaaaggaca ggttatgagc gattttttac
    19861 cggaatttga accgtgcggc atttatagtg gattaacaaa aatcaggaca aggcgacgaa
    19921 gccgcagaca gtacaaatag tacggcaagg cgaggtaacg ccggaccggt ttaaatttaa
    19981 tccactatac ttgggtagcg attgggtttc agacggcata cattcaggat ttcaccgcct
    20041 tataccgtct gaaagacgtt tctgacccat ccctactaca cgctttgtcc gcaaacgtat
    20101 cacaggcaag attatggaag tcatccgata cccaaattcc cccttcaaac tccaccaacc
    20161 cttcccgccc gcaggcgacc agcccaccgc cattgcgggc ctgctcgaag ggctttcaga
    20221 cggccttgcc taccaaaccc tgctcggcgt aaccggttcg ggcaaaacct acacgatggc
    20281 gaacgtcatc gcgcaaagcg gcagacccgc catcattatg gcgcacaaca aaacccttgc
    20341 cgcccagctt tatgccgaaa tgcgcgagtt tttccccgaa aacgcagtgg aatatttcgt
    20401 gtcgtattac gactattacc agcccgaagc ctatgtgccc agccgcgatt tgttcatcga
    20461 aaaagacagc gcgatcaacg aacacatcga gcagatgcgc ctttccgcca ccaaaaacct
    20521 gatgacgcgc gacgacgtga ttatcgtcgc caccgtgtcc gccatttacg gtatcggcga
    20581 cccgaccgag tatcaacaaa tggtgctgtc cgtaaaagaa ggcgacacca tcgagcagcg
    20641 cgacatcatc gccacgctcg tttccatgca gtacgaacgc ggcgatttgg acttcaaacg
    20701 cggcagcttc cgcgtgcgcg gcgacgtgat tgacgtgtac cccgccgaaa gctccgaaaa
    20761 cgccttgcgt atcagcctgt ttgatgacga aatcgaccgc ctcgatatgt tcgacccgct
    20821 ttcaggcagc ctgcaccagc gtgtcggacg ctacaccgtc ttcccgtcca gccactacgt
    20881 taccccgcgc gacaccgtat tgcgcgcctg cgaatccatc aaagaagaat tgcgcgaacg
    20941 catcgaattt ttcgctcgcg aacaacgccc cgtcgagcaa caacgcatcg aacagcgcac
    21001 ccgtttcgac ctcgaaatgc tctacgaaat gggcttctgc aaaggcattg aaaactactc
    21061 ccgccacttc tccggtaaaa aagaaggcga accgccgccc acgctgatgg actacctgcc
    21121 cgacaacgcc atcatgttca tcgacgaaag ccacgttacc gttacccaaa tcggcggcat
    21181 gtacaaaggc gacgcatcgc gcaagcaaaa cctcgtggac tacggcttcc gcctgccttc
    21241 cgcccgcgac aaccgcccgc tcaaattcca cgaatttgaa aaagtcatgc cgcaaaccgt
    21301 cttcgtttcc gccacccccg cgaaatacga agaagaacac gccggacaag tcgtcgaaca
    21361 agtcgtccgc cccacagggc tggtcgaccc ccaaatcatc atccgccccg tcgccaccca
    21421 agtcgacgat ttaatgagcg aaatcaacga ccgtattcaa aaaggcgaac gtgtgctcgt
    21481 taccaccctc accaaacgca tggcggagca actcaccgac tattacagcg aactcggcat
    21541 caaagtgcgc tacctgcaca gcgacatcga caccgtcgag cgcgttgaaa tcattagaga
    21601 tttgcggctc ggcctgtttg acgtactcgt tggcatcaac ctgttgcgcg aaggcctcga
    21661 catccccgaa gtgtccctcg tcgccatcct cgatgccgac aaagaaggct tcctgcgctc
    21721 ccaccgcagc ctgattcaaa ccataggccg cgccgcgcgc aacgtgaacg gcgtcgccat
    21781 cctgtacgcc gacaaaatta ccgactccat gaaagccgcc gtcgacgaaa ccgaacgccg
    21841 ccgcgaaaaa cagattaaat tcaacgaaga acacggtatc gtgccgcagc agattaaaaa
    21901 acaggtcaaa gacatcatcg acggcgtgta ccacgaagaa gacagtggca aaggccgtag
    21961 acaaggcaaa aacaaggtca aagtcggcga aatccacaac gaagaagacg cgattaaaga
    22021 aatcgccaaa ctggaaaaag ccatgcagca ggcggctagg gatttacagt ttgaagaagc
    22081 tgctgtgttg agggatagga ttaggaatat taaagagaat ttgttgtttg gggcggagtg
    22141 attttgaaat agtaatcgca gcctgaatac atagcgtgtg cgccactggc gcgcgctcat
    22201 tgtgttatca ggaatccttg aaatcatgta cgcagcttgc atcacacatc ctagctatac
    22261 ataaacaaca aggccgtctg aattttcaga cggccttttc cgtttacaac tttactactt
    22321 attcaataac tacacgtgca ttcagaggct gtacggggcg gttgttttcc gaaccgacgg
    22381 cgcgggtgaa tttttccgct tgcgcttggt cgatgtggtc ataagttttc aacaccaacc
    22441 atgatacgcc ctctgtgcac ggcggcgtgg tcagcgaacc ggcaaagcgg tagtatttca
    22501 accgtttcgg cagtagggtg gatgcgtcga acggttggtt gagtttcact tttcctgcgg
    22561 tcatcggcat gacgttccag atggaagaca ggcggccgtt ggttttgccg gcttcataca
    22621 gcacggctaa tactaaaggc tgtttgtttt cgtctaagtg gacgaagtga gcttccatcg
    22681 ggaaagtgcg gcctttgatt tggttttcgc tcggcacgtg gaagtggaac tgtttcaggg
    22741 tataggtgcg gccgttcacg gtcagggtat tgccgccttc gggataattg acctgaatgg
    22801 tgtggccgtt gttttccacg tcaaccatac tcggtttgta attgactttg atggcgggca
    22861 gtttgccgga aacggtttcg gtaatgttta ccggagattg gtttttgccg gtggagcaca
    22921 aacggaattc ttctgacaga ttgccccagc tttcgggaga gtcgtgtccg gtatagcccc
    22981 aatgggtgtg attgccgtgt gcggcggcgg agaaagcggt acaggccaac agcaggacag
    23041 cggtcaggcg gggtaaagtg cgggggaaac gaggcattgt aagatccttt ctattaagga
    23101 gtagaaaaaa ggttgcagtg tgtatgtaaa tattttattt tttattaaat gatatctata
    23161 ttaagtgctt aagtcaaact tcccggtctg aaaaccatta gagcaatgaa attaaagtga
    23221 atttattttt ctataagtcc tgctttattt ggataaccat gaggcatggc tacctttatc
    23281 cggaaaaata ttaatagccg caacaaagat atttgaatcc gattcaaata tcaccagcaa
    23341 gcgtagctcg gatacttgta tccgacaaag cctttaacat ttccatcgtt gcaatccatt
    23401 gcagcaatta ccaaaatgtc ggattcgaga atccgatcta cggaaaacta aagattttat
    23461 gcgggctacg gcttgcttgt tgtatttaat catatttatt tgaattgaat tcttttattt
    23521 tattgtgaga tgaaaatagt attttcgatg agaaacccat gactaaaata gtatagtctt
    23581 tatcattctt tacgaagtaa caattaccat atcctgcaat atttttatcc caaaaatcac
    23641 aatatgattc agtggtaaaa tcagttttag ttgatataaa atcaaaatta tttgggtaag
    23701 atccatattg ctggtaatat ttatctatat tttctactaa gaaatcaatc cttttttcta
    23761 acattttaag attaatttgc aataataaaa tgtataaaac tatactaatt gctattactt
    23821 ttaaatatgt cattttattt tccagtaaat agaagtttag ttgttttgtt aactatgcct
    23881 gaatgtacac ctaagtcttc ttttggtaac gatgaataat tagcaagccg tagcctgcat
    23941 ctccgccaac cgcgtgccac tatctgcggg cggaataaac tttaaaaccg atgtgcaggt
    24001 taaaacctac aaggatgcgt gcggtataca tgcgtttttg ccgttcaaac cattgtttca
    24061 aaacttgtat tgaaacatcg cccgctgctt tatcttgaaa gaatgccgtc tgtaagcaaa
    24121 catgcttcag acggcatttt tttatccggc ggtctgttat ccgatccgtt tgaataccaa
    24181 gtcccaaacg ccgtgtccga ggcgtttgcc gcgcgcttcg aatttggttt cggggcggta
    24241 gtccggagtg ggggcgtagt ctgccgccgt attttgcagg ttgtcgaagc tgctgaggac
    24301 ttcaagcatc tgctgtgcat attcttccca gtctgtcgcc aggtggatat agccgccggt
    24361 tttgagtttg ggcagtagtt tggcgatgaa gggggcttgt atcagacggc gtttgtggtg
    24421 gcgttttttg tgccacgggt cggggaagaa tatgtggatg ccgtcgagcg agccgtcttg
    24481 cagcatattt tcgacaactt ctacggcatc gtgccgcatc acgcggatgt tttccaaatg
    24541 gttttcgttg atgagtttga gcaggttgcc tacgccgggg ccgtgtacgt cgatggcgag
    24601 aaagtcggtt tcgggcaggc ggcgggcgat ttctgcgctt gccatcccca taccgaagcc
    24661 tatttcgagg attttaggtc ggctgcttcc gaaacgggca tcaagatcgg ccggtgttgc
    24721 ttggtagtcg atgccgaagc tgtcccataa ggtatcgatg gcgcgttgct gtgcggcggt
    24781 catgtggctt tggcggagga cgaaactgcg gatgctgcgg gggtgtccgg cttgacggtc
    24841 gtttggtgtt tggttttcgg cggtgtctgt cataatgctt ctgtgcgctt gtgcaatcag
    24901 gatgccgtct gaatgtgaaa ggcctgttcg gacggcggga agggaaaggg gcgattatac
    24961 ctgtttcgtt tcggcagggt aggtgtaacg gtgttcagac ggcattttgc cactgttgca
    25021 gcagttcttg caggacgctt ttttgcccgt atccgttgtt tatggcttct tgcaggtagc
    25081 ggcaggcttt ttctttgtcc ggcctggcac cgtgtccgct gtaatggatg caggcgaggt
    25141 tgtagaacgc catactgtct ttttgcgcgg cggcttgttc aaaccatttg cgggctttgc
    25201 agtagtcggc ggctatgccc tgtccgaagt agtacatact gcccagattg gtttgggctt
    25261 tgctgtgtcc gagttctgct gcttctgtgt agagtttgag cgcggctgcg tattcgggcg
    25321 cgcattgtaa tccgtaatgc agggcttggg ctttttgata aagctgttct gccctttgcc
    25381 gcctaatggc ggaatctttg atgccgtcat attgctcggg atggtttaac gcgctgtcgg
    25441 atatgagttt ctgataggcg gtaaggctgc cggcttcggc tgcctgccgg taataatgcc
    25501 gggctttttc cgtatcgagg gctatgccca aaccgtagcg gcagatgtcg cccggtatcc
    25561 gcaaaccttc gggatggcgt tcggcggcgg ctgcttcggc atgatgcagg gcaagtttgt
    25621 ggtttctttc cagatacttg ccttgcaggt agatgtcggc cagtgcggca tgggcatcgg
    25681 ggtcattttc ctgtgccgcc tgttggaacc ggtaaacggc ttcatcggga gccgtaggta
    25741 ggatgagttc ggcaagcggg gtgtaggcgg gaatgtatcc ttgttgcgcg gcggcgcgca
    25801 aatgattgac ggcctgtgcc gtgtcggttt tcgtcccttg tccgaaacgg tattgcagcc
    25861 cgagttgcca atgtgcttcc ggcaagcctt gtgccgccgc tttgcggtag agcaggtgcg
    25921 ccgtttcaaa atccggcggc gtgccgtgcc ggcagtattg ggcgagcagg tattgcccgc
    25981 ggggattgtt gttttttgcg cttgttttgc ccattccagg gcttcttcaa aatgtcggga
    26041 cgcgtacaga aggtacatca ggcggacttg cgcttcgggc aggcctgctt ctgccagagg
    26101 cctgtaaagc ggtatcaggg tttccggggg cgcgccttcc aattcttgga tatatgccaa
    26161 attgtaacgc gcatagggat gcccctgttc ggcggcgatg cggagcattt ccataccttg
    26221 ttcgcggtct tggtgcgggc tgtccgcgtt catcaggtgt tttgccaact tgaatgcggc
    26281 ggcgggatga tctgccgagg ccgcttctgt cagcagtcgg cggacttcgg taaagttata
    26341 atattgtttt tgagacaggc gttccaaggc ctgccgatag ggtaagtcgg gattgttcat
    26401 atcagatcgt tttccgtttt gtcgaatcat cgttattcgt catgaagtta ggtttatatc
    26461 ggtatgccgg cctgctgtat catattttta caggcggttt ttgctttggc gacatactaa
    26521 tgcagatgat tataaatggt ttccgccaat gccttgctga tgccttccac tttttccaag
    26581 tcctcgcggc tggcggcaat cacgccgcgc agtccgccga agcgggtaag cagggcttgg
    26641 cggcgtttgc tgcctacacc gggaatgtcg ccgagagacg aggtaacgcg tgctttgtcg
    26701 cgttttttac ggtggcctgt gatggcaaag cggtgtgatt catcgcgtac ggtttgcaat
    26761 aaatgcaagg ccgggctatt aggaggcagg cggaagagtt cgccggtaaa aggcagtatg
    26821 agttcttcca tgccggcttt gcgttcggtg cctttggcaa taccgactaa agggatgtgc
    26881 agcccgagtt cttcccatac ggatacggct acgccgattt gccctttacc gccgtcaatc
    26941 aacacgacat ccggccattt gacgctttcg ccgttggctt cggcttcctg tattttgcca
    27001 taacggcgcg tcaacacttc gcgcatggcg gcgtagtcgt cgccgggttt ggcggtggtg
    27061 atgttgtagc ggcggtattg cgagggttgg atgttttgtt cgtcgtacac gacgcaggag
    27121 gcgatggtgg cttcgccttg cgtgtggctg atatcgaaac attcaaggcg gttgattccg
    27181 tctgaattca tgccgaggat tttggcaagt tcgtcgatgc ggtgttgttg gttgctttgt
    27241 tgcaggtggc gttgggtaat cgccatttgt gcgttttgtt ccgccatttt caaccaaact
    27301 ttgcgttcgc ctatggtttt ggtaacgaac tgcatctgct tgccgtgttc tccttccaaa
    27361 gcctctttca acgcatcggg aacgggaaag ttgctgatga tgatgtcggg tttgcttttg
    27421 cccagatagt gttgggcgac gaaggcttcg gcgtaatctt gaccgtttgg ttcggggtcg
    27481 tttttggtgt cgggaaagaa gcttttgtcg ccgacgtgcc gtccgccgcg gatgctgacc
    27541 cagtgtacgc agacaaggcc gtctgaaacg gcgagcgcga gcaggtcgat atcgttgggg
    27601 ttgttcgggt ttttgctgtc gatgaactgg tcgctctgta taatgccgag tgcttggatt
    27661 tggtcgcgat aacgggcggc ttcttcaaat tgcagattcg cggcggcggt ctgcattttg
    27721 tgttgcaggg tgcgggtcag ttcgtcggtt ttgccgttga ggaaagtggc ggcttgacgc
    27781 acgctgtcgc agtagtcttc ttcgctgatg tgtccgacgc agggcgcggt gcagcgtttg
    27841 atttggtaca gtaggcaagg gcggtcgcgg tgttcgaaca cgctgtcttc gcaggtacgc
    27901 agcatgaaga ctttttgcaa cacttgaatg ctgtcgcgca cggcgttgct gttcggatac
    27961 gggccgaagt attggttggg atttttcagc gtgccgcggt aatacgccat ttgcggatat
    28021 tgatggccgc tgagcatcaa ataaggatag cttttgtcgt cgcgaaaaag gatattgtat
    28081 ttcggcgaca aggctttgat gaagttgttt tcgagaatca gggcttcggc ttcggaacgc
    28141 gtgatggtgg tttcgatgtg gcgaacctgt ttcaccatca acgcgatgcg cggcgaatgg
    28201 tcgttttttt ggaaatagcc ggacacgcgc cgcttgaggt tgaccgcttt gccgacgtat
    28261 aagacgttgc cgccttcgtc aaaaaaacgg tacacgcccg gcagcttggg caggtttttg
    28321 aggaagagcg ggatgtcgaa ttgttccgga gaacggtttt ctgtattcat atcaatcatt
    28381 tgaaaaacgg cttcagacgg catatcggca gcggcagcct tatccgttcg gaaaagctgt
    28441 cgggcggtta tatgcggtca atcgattttt acccatttca ggcgcagggc attgcccaat
    28501 accgaaaccg agcttgccgc cattgccgcg cctgctatga cgggatttaa aaagccgagc
    28561 gcggcgagcg gaatgcccaa tatattgtag aagaaggcga aaaataggtt ttgcttgatg
    28621 ttttccaacg ttgcctgcga tatcagcagg gcatcggcga gctgattgac cgaatgctgc
    28681 atcagcgtgg cggaggcggt gtgttcggca acgtccgcac cgcctttcat ggcgaagctg
    28741 acgttggcgg cggcaagcgc gggcgcgtcg ttgatgccgt cgccgaccat cgccacggtt
    28801 ttgccggcgg ctttgagttt ctgcacttcg gcggctttgt cgcacggact catattaccg
    28861 aaggcgtgtg cgatgcccag ttgtttggcg acgtattcga ccgtactttg gttatcgccg
    28921 ctcataatat agacatcgat attgtgtttt ttcagacggc ctatggcttc ggcggtatcg
    28981 gctttcaacg cgtcggagag tgcgaatgcg ccgatcggtt tgccgtttac agatacggta
    29041 accgcactgg cgatttccca aacgccgtct gaaaacttcg gcaaggtcag ttcggcaaat
    29101 tcggctttgc ctgatttcac caaacccacg ccttccactt cggcggtaat gcctgctccg
    29161 acaacggttt gcgcattttg tgcagcggga atctccaaac cgcgcgcttg tgcggcggag
    29221 acgatggcgc gggcgagcgg gtgggcggcg ttttgctcga cggcggcggc gatgcggtac
    29281 aaagcgtctt cgtcaaagcc gctgtcggga acgtaataaa cggcggcaac ctgcggcctg
    29341 ccttcggtca gcgtaccggt tttgtccaat acgacggcat cgacgtgggc tgcttcctcc
    29401 attgccgccg cgtctttaaa ccaaatgccg tgtttcaccg ctttgcccat gccgaccata
    29461 atcgcggcag gggtcgccag accgagcgcg cacgggcagg caatcaccaa aacggcaacg
    29521 gcgtgcatca gtgcgaccgt ccaatcgccc ttaatcagcc aagtaacgat aaaagtcaga
    29581 agcgcgatgc ccacgacagt tggcacaaat accgccgccg ctttatcggc cacgcgcgca
    29641 atcggtgctt tactgccttg tgcttcagag agcgcgttca tcatgtcgcc gagcagggtt
    29701 tggctgccga gctgcgcggc gcggtacacc acgctgcctt cggtcatcag cgcgcccgcc
    29761 aacactttgc cgcccgcctt tttctcttcg ggattggatt cgccggtaag gtggctttcg
    29821 tccgcccaac cgctgccgct ttcgataatg ccgtcggcag cgatgcgttc gccgtggttg
    29881 gtgcggataa ggtcgccgat ttgcacttgg tcgatgggca gttgtttcca ttcgccgttg
    29941 cgttgcacgt tgacttgggt cggcgtgagt tttagcagta agcccaagct gttcaggctg
    30001 gattttttgg tgcggtgttc caaaaactta cccagcgaca caaaaccgat caccatcacg
    30061 cccgcttcaa aatacacatg cgccataccg tgcgccgcat gcgaactgaa aaacagcata
    30121 taaacggaat acaggtaaat cgacaccgtg ccgatggtaa cgagtacgtc catattcgcc
    30181 agcccgcctt taatgcttgc ccacgcgctt ttgtaaaacg ggattgccag ccaaagttgc
    30241 actatgcttg ccagtacaaa ctgccataca ggcggaatca tccaatcgtg ccgtgtccaa
    30301 ttcagccctt ttagcatcat ccctaccata ccgataagga acgggatatt gatggtcagc
    30361 aaaagccaca accgccagcc gatatggtgt tctgcttcag gttgcggcaa tgtatcttcc
    30421 gttttttcct tcgcgccgta accggttttc tcaatgattt tggcaatgtc ggcaaccgag
    30481 gttttgctgc cgtcaaacgt aacctgcgct tcctcactgg caaagttcac tcccgccgat
    30541 tcgacaaaat cttttttgtt caacactttt tcaatgcgcg aagcacatgc ctgacaggtc
    30601 atcccctcga tttggaaacg gattttttgt tgcataagtt tgattcccgt tttgatgttc
    30661 agacggcagt caaggctgcg ggatgccgtc tgaacgccgg tatggtcaaa atttctcaaa
    30721 cgcgccgata attcgggtag aatccgaaaa cttgacacag caaaggaaaa acaatgtccc
    30781 aacaccgcaa actgattatt ttgggttccg gccccgccgg atacaccgcc gccgtctatg
    30841 ccgcacgtgc caatttaaac cccgtcatta ttacaggtat cgcgcaaggc gggcaactga
    30901 tgacaacgac tgaagtggac aactggcctg ccgatgccga cggcgtgcaa gggccggaat
    30961 tgatggcgcg gtttctcgcc cacgccgaac gtttcgggac agaaatcatt tttgaccaaa
    31021 tcaacgccgt cgatctgcaa aaacgcccgt tcgcactcaa aggcgatatg ggcgagtaca
    31081 cttgcgatgc cctgattgtc gccaccggcg cgtccgccaa ataccttggt ttgccgagtg
    31141 aggaagcgtt tgcagggaaa ggcgtttccg cctgcgccac ctgcgacggt ttcttctaca
    31201 aaaaccaaga tgttgccgta gtcggcggcg gcaatacggc agttgaggag gcactctacc
    31261 ttgccaatat cgccaaaacc gttacgctga ttcaccgccg cagcgagttc cgtgctgaaa
    31321 aaatcatgat tgacaaactg atgaaacgcg tggaagaggg caaaatcatc ctcaagctgg
    31381 aaagcaacct gcaagaagta ctgggcgacg atcggggcgt aaatggcgca ttattaaaaa
    31441 acaacgacgg ttctgatcaa caaattgccg tcagcggcat ctttatcgcc atcgggcaca
    31501 agccgaatac cgatattttc aaagggcagt tggaaatgga cgaagccggc tacctgaaaa
    31561 caaaaggcgg cacggcggac aatgtcggtg caaccaatat cgaaggcgta tgggcggcgg
    31621 gcgacgtaaa agaccatacc taccgtcagg caattaccag cgcggcctcc ggctgtcagg
    31681 cggctttgga cgcggaacgt tggctgggca gccaaaacat ttgaatgccg tctgaaaccg
    31741 aaattttcgg cggaatgcaa gtttttcgta ccaaatcctt gattccgtcg gcaaacccca
    31801 atataatgcc tgctctttga accttgcctt ctgctttcgc atggcagaag gctgttttta
    31861 ccatccataa ggagataaca tgcgtcatta cgagatcgtg tttatcgttc atcctgatca
    31921 aagcgagcaa gtgcccgcta tggttgaacg ttacaaaacc atgattaccg aggcaaacgg
    31981 taagattcac cgtttggaag attggggccg ccgccaactg gcttacccga ttaacaaaat
    32041 ccataaagca cattatgttt tgatgaacat cgaaaccact cccgaagttg ttgaggagtt
    32101 ggaaaccgcg ttccgtttca atgatgcggt attgcgtcat ctgaccatca aaaccaaaca
    32161 cgccgttacc gaagcctccc ctatgctggg cggcgaaaag gctaagaacc tgttggccgc
    32221 cgcggctgaa gaagcggctg cccaataatt gggattcact aatcttgttt cgcttgccgc
    32281 attgattgaa aaggctttcc ctattcgata tacgcctgcc ggaatccctg ttttagatat
    32341 tattttaaag cacgaatcgt ggcaggagga aaatgggcag caatgccttg tccaattgga
    32401 aatccccgcg cggattttgg gtaggcaggc ggaagagtgg cagtatcggc aaggcgactg
    32461 cgcaacagtc gaaggttttt tagctcaaaa aagcagacgt tccctgatgc cgatgctcag
    32521 gatacaaaat attcaagaat ataaaggtta aacgacaatg gctcgtcaat cattcaaacg
    32581 tagaaaattc tgccgtttca cggctgaaaa aatccaagaa gtcgattaca aacaagttga
    32641 tttgctgaaa gactttatct ctgaaaacgg taaaatcatt cctgcccgca tcacaggaac
    32701 gaaggcattc taccaacgcc aattggctgt tgccgtaaaa cgcgcgcgct tcttggcact
    32761 cctgccttat accgaccaac acaaataatt ttggagttta aatcatgcaa attattctgt
    32821 tagaaaaaat cggcggtttg ggtaatttgg gcgacatcgt taccgtaaaa aacggttatg
    32881 cccgcaactt cttgattcct gctggtaagg caaaacgtgc tactgaagca aacatgaaag
    32941 agtttgaagc acgccgcgcc gaattggaag ccaaacaggc cgaaattttg gcagatgccc
    33001 gagcccgtca ggaaaaactg gacggtcaaa ccgttaccgt tgcacagaaa gccggtgtgg
    33061 acggccgcct gttcggttcc gttaccaatg ccgacattgc tgccgcaatc gttgctgccg
    33121 gcatcgaagc cgtgaaagca aatgtacgtc tgccgaacgg tcctctgaaa gccgttggag
    33181 agtacgaagt ggaagtggct ttacacactg atgccgttgc taaaattact gttgccgtca
    33241 ttgccgcagc cgagtaattg aaattatcag atgccgtctg aaaccctgcc gattatcaga
    33301 atgtggtttc agacggcatt tgttttgttt tccgtatatt ctgcaaaaat cgggacggtt
    33361 gttttttatt tgcctgaggt ctttgcagaa atggtttgtt aacgaaattt gatgcataaa
    33421 atgcgccaaa aaatattggc tgtgttttaa tatagtgttg ataagcgtac ctggtttcaa
    33481 tatattcaat aggataggtc tcttcccaac cgaaaaaata attgtctttt ctcttgtaaa
    33541 tagttgaaat agattgtcaa aatacatcct aaaaaattta ataacttata tgattgcaaa
    33601 ggttgcggct tgtatgccgg tcattatggc ggaaataccg tttgggaggc agtcgcatcg
    33661 catggagtcg gcacggcagg cgatgcgccg atgccttcgg gcgtgaaggc agggaaatcg
    33721 gcagtttgga aaaaggcaat ggaatccgca ggtaggtgtc gattgggtgg tgaaaggcaa
    33781 taagtcgggc gcacgctcag actgcctatc cccttgtcgg aacgggaggt agggcagctt
    33841 tgtcctgact aattgcttgt gcaaacggta agaaggtttt taaagcaggt tgtacagcat
    33901 ttttccggca atcaaaagca acataatgcc gaaggattct ttgagtttgg cagaagaaag
    33961 tttgtgggcg gttttgacac cgagcggggc aaaggcaata gttgccgcgc tgaggacggc
    34021 gacggcgggc aggtaaagga agcccagcga cccttcgggc aatcctgcaa tattcagacc
    34081 gttgaccaga tacgatattg cgccggagag tgcaatcggc caggcaaggc cggatgatgt
    34141 gccgatggct ttatgggcgg ggaagccgca gtggattaag aaggggacgg aaagtgaacc
    34201 gccgcctatg ccgacccagc tcgacattgc gccgaacagt gtggaaaccg cagtcagtcc
    34261 gggcagcccg ggcagcgggc gggatgccgt ctgacgaccg gtatgcagtg ttttgaatgc
    34321 gacggcggtt aaaaacagga tgaagaaaat ttgaagcccg aacgcgggga tatattttgc
    34381 ggagagtgcg ccagcgaata cgccgaatat catacccagc atcatcgcaa atatggtttt
    34441 ccagtcgacc gtctgttttt tgtgctgccc caacatactg gaaaaggcgg tgaagaccat
    34501 gacggcgaag gatgtgccga cggcgaggtg ttgcgcgtaa ggatgttgtg ccaaaccctg
    34561 caaatcaagc acccataaaa cgacagggac aatcagcgta ccgccgccta caccgaacag
    34621 gccggcaata aaacctgccg cactgcctac ggcaagcagg attaagataa tgtcccaatg
    34681 ccacatttaa tccgcctttc tgtatttttt ccaaaatcgg cgtgccgcca taatgtagcc
    34741 ggacaggctg tatccgagaa agaacaggaa aaggacgagc gacggttccc aagtgaccag
    34801 aagcagcagt aagacggcaa gcagcattcc gacaaagggg acttgtctgc ggatgttgat
    34861 ttctttaaaa ctccaaaaag ggatttggac aatcatcgac aggccggcaa acagtgtgat
    34921 gcctaatgcc caccagtgga cggcggggaa tttttcgacg ctgtggttga cccaaatcag
    34981 cccgacaatc agcgcggcgg cagtcggact gggtacgccg ataaaccagc gtttgtccac
    35041 cttgccgatg agcgtgttga acagcgcgag gcgtagggcg gcgcaggcgc agtagatgaa
    35101 ggcgacggaa taaccgattt tgccgaactg ccaaagctgc catttgtagg caataagcgc
    35161 gggggcaacg ccgaagctga ccatgtcggc aaggctgtcg agctgctccc cgaacgcgct
    35221 ttggctgttg gtcagccgcg ccacgcgccc gtccataccg tccagcaaca tagagatgaa
    35281 taccgcgatg gcggcggttt cataacgtcc gtgcatggat tgggtgattg cgtaaaatgc
    35341 ggaaaacagc gcggcgatgg taaaggagtt gggcagcagg tagatactgt tttgacgcag
    35401 ggagcgttgc ggggtgttgg tcggggtttc catagctgaa gtgtctcacg gacgtggaga
    35461 atcgataggg gattataacc caaaccatta tcgaacaata cgccctttag gatgaagccc
    35521 tcggaggcgg tttgtttttc tgatggcgga tgctttgcgg ggcggtttga ctctgcctgc
    35581 caccgggtgt gttgtttcgg attttgccgg atggatattg ttgccgggca tttctgcgct
    35641 tgccggaagg tatcggaggg agggcatcgg aatatgcggc gcgccgttca aaccggggcg
    35701 gtatttccct attttcaggg agtttgtagg tttcaacaaa ttgaaagata ttttttgcat
    35761 ttccgaccgt atgctgattt tgaagcggga taaattatag gacaaggttt ttcgcccgcc
    35821 gcaaaggttg cccgcaatgc cgtctgaaaa caacggcttg ccgctaaggc ggtggtatgc
    35881 ggatttgcgc tgccgaaacc ggtttgctac aacccggcag tgaaatctgc agggagggat
    35941 aggtcgataa ggcgggactt atcttgtttt ttgtttataa tgcaacagaa tttggaggaa
    36001 tcgtcgggaa tttatcatga tgaaaaagat actggccgta tcggcactat gcctgatgac
    36061 tgcggcggca cgggctgccg atacttacgg ctatctcgcc gtttggcaga atccgcagga
    36121 tgcaaacgat gttttgcagg ttaaaaccac aaaagaagat tcggcgaaaa gcgaagcgtt
    36181 tgccgagttg gaagcctttt gcaaaggtca ggacacgctt gcgggcattg ccgaagacga
    36241 gccgaccgga tgccggtcgg tcgtgtcgct gaacaatacc tgtgtctcgc tggcataccc
    36301 gaaagccttg ggcgcgatgc gcgttgaaaa cgccgtcgtg attacttctc cgcgttttac
    36361 gagcgttcat caggtcgcac tcaaccagtg cataaaaaaa tacggcgcac agggacaatg
    36421 cggcttggaa acagtgtatt gcacgtcatc ttcttattac ggcggggctg ttcgctcctt
    36481 aatccaacac ctgaaataaa aaatgccgtc tgaaggatgt tcggacggca tttccatatc
    36541 gacggtcagg attctttcgg atcgggcagc aggctgttca acacaatggc gagtacggcg
    36601 cacaagccca cgccgacaaa gctgaagctg cccaatttga gtgtcatacc gccgatgccc
    36661 gtggtcagta ccgagctgac gatgaccagg tttttcggca gcatcaaatc gactttggca
    36721 tcaatcaggg ttttgatgcc taaagaagca atcgtgccga acagcagcag cataatgccg
    36781 cccatcacgg gcatcggaat ggaagccaaa aacgcattga atttgccgaa aaacgccatg
    36841 cagacggcaa aaaccgccgc ccaagtcatg atgacggggt tgctgttttt ggtaatcatc
    36901 accgcacccg ttacttcgcc gtaggttgta accggcgggc cgccgatcag acctgcaacg
    36961 cacacgccca agccgtcgcc cgcaagggtt ttgtccaagc ccgggtcttt tgtgtagtct
    37021 tttcccgtta cattgccgat tgccatgatg ccgccgatgt gttcgatggc gggggcgacg
    37081 gcaacgggca gcataaacag tgccgcctgc cagttgacct gaggcgtttc aaaatgggga
    37141 acggcgaacc agggcgcgtg tgcaatgctt gtcgtgtcca ccagtcccat cagcagtgcc
    37201 aaaacataac ccgaagcgac accgatcaag atgggaatca gcttcatcat cctgctgccg
    37261 aaaaccgata cgatggcggt aacggcaaag gtaaagccgg aaagaatcag cgaatcggta
    37321 tagtcgatga cctgtttgcc gtccgcctga cccattgcca tgctgcttgc cgccacggca
    37381 acagacagac cgatgaccat gatgacgggg ccggtgacga ccggaggcag cagtttgtgt
    37441 accgctgcca gtccgcgcca acggatcagc gcggcaaaca caaaatacat aaagccggcg
    37501 gcaaacagtc cgaacatggt ggaaggcagc ccccattcgc cgacggagta gataatcggt
    37561 gcgataaagg caaacgaaga accaagaaaa atcggcactt tgcgtttggt tgtgatttgg
    37621 aacagcagcg ttcccaagcc tgcgcccaaa agcgcaagag ccggattcag accggtcagc
    37681 aggggaacca gcaccattgc gccgaatgcc acaaataaaa tctgcgcgcc ggaaacggca
    37741 agtttcagtt ggttcatacc atcttctttc aatcggttca aacggcacgg attatactgg
    37801 tttgccgagg ggctgcgtaa aaatacatac gtcgggcaaa gatatgccgt ctgaaaggca
    37861 acgtctgtct gttgcaggat gaaagcagga gaaatggagt ataatccgac actttccata
    37921 ttcaatccgc aagccgtccc gaacccttat ttcagacggc attttccggt aatacggcat
    37981 gacagaaaaa tcccataaaa aaacagcaaa aggcagggcg ggaagcccgt ccccaacgtc
    38041 cgcacgcaac aagaaagccg acaacggcgc gcggggcaat aaagtttccg agcggctcaa
    38101 ggcggtcaaa gagctgcaga aaaccgaaac caaaaaggcg cgtcccgaac atgtcgtcaa
    38161 cctgattgcc gacgcgctgt ggctgatggg tttggcggca accctgtatt tggcgatttc
    38221 cctgatcagt ttcgatatgg gcgatccgtc ttggtcgcac agttcaccgg ttgtggaaga
    38281 tgccgccaat tggggcggac tgttcggcgc gtatgttgcc gatgtcggct attatctttt
    38341 cggctggtcg ttctggtggt ggatagcggc tgcctgcgtc gtgctgtata aaaatttccg
    38401 tctgcacgca aaacagacgg aaaacgaggc atacaaccac aaaatcgctg ccgccgcgct
    38461 gtttgtcctg acggtcttca gccccgtctt ggagtatttt gtgctgggcg gaaaatatgc
    38521 cgactccctg cctgtcggag caggcggtat ggtcggcata cgtgtcggcg cagtgtttgc
    38581 gtggctgttg gggaaatcgg gcagcctgct gattatcctg gttgttctgc tgttgtcgtt
    38641 gtccctgctg gtgcagattt catggctgga atttttgaac ggtgcgggca gggcggttca
    38701 aaaccgcctg agtgccttat ccggcaaggt catggctttg agaaaacgcc ggccgaatac
    38761 caaaacagac ggtgtcgata cccaaaatac acggcgcatg gtgaaagaag ccaaaaacat
    38821 cactgccaaa cccgttgcct tgcccgaagg cagcagcagc aaccgcaaat ccgtcgcggt
    38881 ttccgttgcg ccgccgccta aaatccaacc ttcgctgttt gaagacaatg aagtacagca
    38941 gaacggcgaa taccacaagc ctacattgaa cctattgcgg attcccgaca gcgagccggt
    39001 cagcatcaat cccgccgaat tggagcgcac tgccgaacta atcgaatcca aactggcaga
    39061 attcggcatc ggcgtacaag tcgtatccgc gacatccggc cccgtcatca cgcgctacga
    39121 aatcgaaccc gcgcaaggtg tcaaaggcag ccaaattgtt gccctgtcga aagatttggc
    39181 acgctctatg tcgctgcaat ccgtgcgtat cgtcgaaacc attgcgggca aaaacacgat
    39241 gggcatcgag ttgcccaacg aaaaacgcca agacgtgatg ttgagcgaaa tcttgtcctc
    39301 gcccgtgttt gccgaagcca aatccaagct gaccgtcgcg ctgggcaaag acatcgccgg
    39361 tacgcccgtc gtcggcgact tggcgaaaat gccgcacctt ttggtcgccg gcatgaccgg
    39421 ttcgggcaaa tccgtcggcg tgaacggcat gattatgtct atgcttttca aagctacgcc
    39481 cgaagaagtc cgcttcatta tgatagaccc gaaaatgctc gagttgagca tttacgacgg
    39541 cattccgcac ctactctgtc ccgtcgttac cgatatgcgc gaagcagggc aggcgttgaa
    39601 ctggtgcgtc gccgaaatgg aaaaacgcta ccgcctgctc tcccacgccg gtgtacgcaa
    39661 tctggaaggc ttcaaccaaa aagtcgaaca agccaaagcg gcaggcaagc cgctgctcaa
    39721 tccgttcagc ctgaacctcg acgaacctga gccactggaa aaactgccga tgatcgtggt
    39781 cgttatcgac gaacttgccg acctgatgat gaccgaacgc aaagctgtcg agcagcaaat
    39841 cgcccgcctc gcccaaaaag cgcgcgccgc cggcatacac atgattgtcg ccacccaacg
    39901 cccaagcgtc gatgtcgtaa ccggtctgat taaagccaac atcccgacgc gtatggcgtt
    39961 taccgtgcaa agcaaaatcg acagccgtac catcctcgac caaatgggcg cggacgaact
    40021 gctcaaatat ggcgattcgc tgttcctgca accgggcagt gccgaaccga cccgcctgca
    40081 aggcacgttt gtttcagacg gcgaagtgca tcaagtcgtc aactacgtca aatcgcaagc
    40141 cccagccgac tatattgaag gtctgctcag cggcgaagcc gcgctggaaa ctgccaatat
    40201 cgttaatccg aatgcagaca gcgacgaatt attcgatcag gcagtcgcct atgttttgga
    40261 aagcaaaaaa acctccattt cgtctttgca gcggcagttg cgtatcggct ataaccgcgc
    40321 ggcaaacctg atggaggcac tggaaaatgc gggtatcgtt tctccctccg acttcaacgg
    40381 cagccgtaaa attttggcgc acaaggacca tttatagccc gtatcgcaaa atgccgtctg
    40441 aacggcggaa ttggcgtttc agacggcatg aggcctttgc aaaaaagccc ttccttcgac
    40501 agccgaaacc caaacacagg ttttc
//
LOCUS       DAXFHF010000011        39109 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXFHF010000011 DAXFHF010000000
VERSION     DAXFHF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39109
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.011"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(38..582)
                     /locus_tag="ACFIRM_000489"
                     /pseudo
     CDS             complement(38..582)
                     /locus_tag="ACFIRM_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(578..700)
                     /locus_tag="ACFIRM_000490"
                     /pseudo
     CDS             complement(578..700)
                     /locus_tag="ACFIRM_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(921..3227)
                     /gene="topA"
                     /locus_tag="ACFIRM_000491"
     CDS             complement(921..3227)
                     /gene="topA"
                     /locus_tag="ACFIRM_000491"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC3028189.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPGNGFAMKYQLISRNGKHVDAIVAAAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQAGVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQVELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIELPAPQE"
     gene            complement(3299..3760)
                     /locus_tag="ACFIRM_000492"
     CDS             complement(3299..3760)
                     /locus_tag="ACFIRM_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC3028190.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            complement(3786..4973)
                     /gene="dprA"
                     /locus_tag="ACFIRM_000493"
     CDS             complement(3786..4973)
                     /gene="dprA"
                     /locus_tag="ACFIRM_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC3028191.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWHNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTVAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            complement(5064..6341)
                     /locus_tag="ACFIRM_000494"
     CDS             complement(5064..6341)
                     /locus_tag="ACFIRM_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC3028192.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            complement(6334..8454)
                     /locus_tag="ACFIRM_000495"
     CDS             complement(6334..8454)
                     /locus_tag="ACFIRM_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3028193.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILARSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            complement(8454..9053)
                     /locus_tag="ACFIRM_000496"
     CDS             complement(8454..9053)
                     /locus_tag="ACFIRM_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC3028194.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            complement(9019..10278)
                     /gene="rsmB"
                     /locus_tag="ACFIRM_000497"
     CDS             complement(9019..10278)
                     /gene="rsmB"
                     /locus_tag="ACFIRM_000497"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC3028195.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            complement(10357..11283)
                     /gene="fmt"
                     /locus_tag="ACFIRM_000498"
     CDS             complement(10357..11283)
                     /gene="fmt"
                     /locus_tag="ACFIRM_000498"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC3028196.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            complement(11371..11874)
                     /gene="def"
                     /locus_tag="ACFIRM_000499"
     CDS             complement(11371..11874)
                     /gene="def"
                     /locus_tag="ACFIRM_000499"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC3028197.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            12058..13281
                     /locus_tag="ACFIRM_000500"
     CDS             12058..13281
                     /locus_tag="ACFIRM_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC3028198.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     GISGKYLYSPWQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KISPDKEVSGYGIPAIDVNFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
     gene            complement(13686..14351)
                     /locus_tag="ACFIRM_000501"
                     /pseudo
     CDS             complement(13686..14351)
                     /locus_tag="ACFIRM_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14414..14872)
                     /gene="pyrI"
                     /locus_tag="ACFIRM_000502"
     CDS             complement(14414..14872)
                     /gene="pyrI"
                     /locus_tag="ACFIRM_000502"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC3028199.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            complement(14882..15802)
                     /gene="pyrB"
                     /locus_tag="ACFIRM_000503"
     CDS             complement(14882..15802)
                     /gene="pyrB"
                     /locus_tag="ACFIRM_000503"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC3028200.1"
                     /translation="MPNPLYRQHIISISDLSREQLEYLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            16038..16880
                     /locus_tag="ACFIRM_000504"
     CDS             16038..16880
                     /locus_tag="ACFIRM_000504"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3028201.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            17582..18272
                     /locus_tag="ACFIRM_000505"
                     /pseudo
     CDS             17582..18272
                     /locus_tag="ACFIRM_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(18548..19099)
                     /locus_tag="ACFIRM_000506"
     CDS             complement(18548..19099)
                     /locus_tag="ACFIRM_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028202.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNPRYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAGKADG"
     gene            19337..20809
                     /gene="pyk"
                     /locus_tag="ACFIRM_000507"
     CDS             19337..20809
                     /gene="pyk"
                     /locus_tag="ACFIRM_000507"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC3028203.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            21043..22395
                     /locus_tag="ACFIRM_000508"
                     /pseudo
     CDS             21043..22395
                     /locus_tag="ACFIRM_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(22478..22900)
                     /locus_tag="ACFIRM_000509"
     CDS             complement(22478..22900)
                     /locus_tag="ACFIRM_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028204.1"
                     /translation="MAGCLTDLENCLEKYLEQFGPVSESIEACTAKLQEQPSFFNRLM
                     KANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHNRKADYAEKGKERIAL
                     FPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(23078..23227)
                     /locus_tag="ACFIRM_000510"
     CDS             complement(23078..23227)
                     /locus_tag="ACFIRM_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028205.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(23245..23442)
                     /locus_tag="ACFIRM_000511"
     CDS             complement(23245..23442)
                     /locus_tag="ACFIRM_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028206.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(23452..24468)
                     /locus_tag="ACFIRM_000512"
     CDS             complement(23452..24468)
                     /locus_tag="ACFIRM_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC3028207.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(24567..25781)
                     /gene="gltS"
                     /locus_tag="ACFIRM_000513"
     CDS             complement(24567..25781)
                     /gene="gltS"
                     /locus_tag="ACFIRM_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC3028208.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(26034..26315)
                     /locus_tag="ACFIRM_000514"
     CDS             complement(26034..26315)
                     /locus_tag="ACFIRM_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028209.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(26334..27458)
                     /locus_tag="ACFIRM_000515"
     CDS             complement(26334..27458)
                     /locus_tag="ACFIRM_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3028210.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(27521..28528)
                     /locus_tag="ACFIRM_000516"
     CDS             complement(27521..28528)
                     /locus_tag="ACFIRM_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC3028211.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYCKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(28521..29522)
                     /locus_tag="ACFIRM_000517"
     CDS             complement(28521..29522)
                     /locus_tag="ACFIRM_000517"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3028212.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(29553..31826)
                     /locus_tag="ACFIRM_000518"
     CDS             complement(29553..31826)
                     /locus_tag="ACFIRM_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC3028213.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASTVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            31941..32957
                     /gene="galE"
                     /locus_tag="ACFIRM_000519"
     CDS             31941..32957
                     /gene="galE"
                     /locus_tag="ACFIRM_000519"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC3028214.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            32958..33998
                     /gene="rfbB"
                     /locus_tag="ACFIRM_000520"
     CDS             32958..33998
                     /gene="rfbB"
                     /locus_tag="ACFIRM_000520"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC3028215.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            34103..34969
                     /gene="rfbA"
                     /locus_tag="ACFIRM_000521"
     CDS             34103..34969
                     /gene="rfbA"
                     /locus_tag="ACFIRM_000521"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC3028216.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPKRFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKHLEKT
                     AYGQYLLHLIGK"
     gene            35107..35664
                     /gene="rfbC"
                     /locus_tag="ACFIRM_000522"
     CDS             35107..35664
                     /gene="rfbC"
                     /locus_tag="ACFIRM_000522"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC3028217.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(35708..37726)
                     /locus_tag="ACFIRM_000523"
     CDS             complement(35708..37726)
                     /locus_tag="ACFIRM_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC3028218.1"
                     /translation="MNKSLSGSVEKYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLVLLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(37921..39042)
                     /gene="dnaJ"
                     /locus_tag="ACFIRM_000524"
     CDS             complement(37921..39042)
                     /gene="dnaJ"
                     /locus_tag="ACFIRM_000524"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC3028219.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         8993 a        10981 c         9896 g         9239 t
ORIGIN      
        1 ttatcagatt tgttttgccg ggatatgatt tgttttgtta aagaatgtat ttatttttta
       61 aaatcccggc caaccgggat aaatcctgct ttaccaattg ttttaaaatg gaaattcgaa
      121 cttttacccc actgttgtca aaacgccgtc cgcactcctt caaatacagc ccgaaatgct
      181 ctttgggaat gccgtcaaac ttgcgcaaat gacgtttgcc cgattccaaa agttcccaat
      241 tgcgttgata tggttttgcc gttcggcaaa atgtgtgccg cgattgatac ggaaacggcc
      301 aaatccgctt acaatctgat acatcgtaac tacgaacaat ccgtataaac aatgctgccg
      361 ggtttcaccc gtccgcggat aataagcaat aaaatagcgg ttcgagtgtc cgacagtaac
      421 cgtacaaacc ttgccgttgc gcttcaaaag cccgaatacg gcggctttgc cggcggcact
      481 gcggccgcgt ctgcctttac ggaaataact ttcacccgcc cctgcttcgc cgtcatacat
      541 ttccaaatga ggactgtttt gacggataag taatcgtaaa cgatatgtcg tatgcctcca
      601 taatttcgca gatttcgtcc aaatgcgtgt aaaggaagtt ttcccgatga tgtgccaaac
      661 actatttcgc catagaaaat aacaggctgc cgtgttttga ttaactgctg ccaaccctgc
      721 tgccgttctt gcagttaccc ctgcggcaaa cagtccgatg ggtttctttt gtttgtaccg
      781 gcttggacgg cttttctcat agggataatt ctgacttaat ttaaatttcc ccagccatct
      841 gggacagccc cttctaattt acaaatgccg tctgaaaccc ctttcagacg gcattttctt
      901 tcatcttaat cccaaccgct tcactcttgc ggtgcaggca gttcaatctg ttctttccag
      961 ccgcattctt tttgcggaca gactttttcc acaccccggc gtttagtggt tttgatggtc
     1021 aagaccggcc aatggcagtt cgggcattct tcggcaacgg gcgggttcca agtggcgtag
     1081 ttgcagtcgg gataggtgct gcaactgtaa aacagtttgc cgtagcggga tttgcgctcg
     1141 acgaggttgc cttttttgca ttgcgggcat tggacgccgg tatctttggg tttttccaac
     1201 ggctcgacgt gtttgcattt ggggtagttg gcgcaaccga tgaatttact gccggtgcgg
     1261 ctgtatttgt acaccagccg tccgccgcat ttggggcatt cgcgaccgtc gagttcgacc
     1321 tgttcggctt ccgctttggc gatgcgttcg gcagcttctt cggcggtttc gttgacgttg
     1381 cgggtgtaac tgcactcggg ataaccggca cacgcaacga aacggcccat tttgccgaac
     1441 ttgatttgca gtttgtgttc gccgcatttc gggcaggttt cgtcaagttc ctgcgtagtg
     1501 aatttggcgc gttcgatgcc ctctttttct tccacttgtt tgatgaacgg tttccagaat
     1561 ttgtccatca cgggaatcca gcggcgtttg ccgtcggcga tttcgtcaag ctggtcttcg
     1621 agtttggcgg tgaagtggta atcgacgtat tgggcgaagt gttcggtcag gaatttattg
     1681 acgatgtcgc ctgtgtcggt tggcatgaag cgtttttgct caagggtaac gtattcgcgg
     1741 tctttgagcg tggagatgat gctggcgtag gtcgaggggc ggccgatgcc gtattcttca
     1801 agggctttaa ccagtgtggc ttcgttgtag cgcggcggcg gggtggtgaa gtgttgttcg
     1861 ccgtagagtt tgtcgaccgg caatttgtcg ccttcgctca tttcgggcag ttttttgccg
     1921 tcttcgctct cttcatcgtc gctgctttct tcgtaaacgc tgaggaagcc tgcgaaggtt
     1981 tgcacttgtc cggttactcg gaacacgcct ttgccgacgg taatatcgac ggtggtttgg
     2041 tcgaatttgg cgggcgtcat ctgacaggca acggtacgct gccaaatcat ttgatagagt
     2101 ttgaactggt ctgcactcag gaagggtttg acgctttcgg gcgtgcggta cacggaagtc
     2161 gggcggatcg cttcatgggc ttcttgggcg tttttggatt tggttttgta ttgtttggcg
     2221 gcactcggca gatattcttt gccgattttg ttttcaatgt aatggcggat ttcggttaac
     2281 gcttcgtcag ccaaattcac gctgtcggta cgcatatagg taatcagacc gatggcgccc
     2341 tgccctacgt ctataccttc gtaaagctgc tgggcggtac gcatggtgcg gtcggtggtg
     2401 aaaccaagtt tgcgcacggc atcctgctgc atggtggatg tggtgaacgg cgcggcgggg
     2461 ttgcggctgc gctttttctt ttcgatggcg gtaacgacgg cctctttgcc ttcgagttct
     2521 ttcaacacgc cggcttgagc ggcttcgtcc ggcaggtcga attgttcgag tttcgcgccg
     2581 ttgtattggg cgagtttggc ggtgaacttg ctgcggcctt tatggctgtc gagatgcacc
     2641 gtccaatatt cctgcgcttc aaacgcgcgg atttcgtttt cgcgttcgca aatcaggcgc
     2701 aaagcagggc tttgcacgcg gcccgcgctc aaaccgcgtc ggattttttt ccacagcaac
     2761 ggcgagaggt tgaaaccgac cagatagtcc agagcgcgac gggcttgttg cgcatcgacc
     2821 aagtccattt cgatttcgcg aggatgggca accgcatcga gcacggcgtt tttggtgatt
     2881 tcgtggaaca cgacacgctg cggcttgatg tttttcaggc cgcgtttgga tttgaggatt
     2941 tccaaaagat gccaagaaat cgcttcgcct tccctatccg ggtcggttgc gaggtagatg
     3001 ttttcggctt ctttggcagc ggcaacaatc gcatcgacat gtttgccgtt gcggctgatg
     3061 agttggtatt tcatcgcaaa gccgttgccg ggatcgaccg cgccgctttt ggggacgagg
     3121 tcgcggacgt gtccgtagga cgcgaggatt tcaaaatcgc cgcccaaata ttttttcagg
     3181 gttttggctt tggacgggga ttcgacgatt aagaggtttt tcgccatttg tcgttctctg
     3241 gatggtggtt ttgatgccgg tgtttcagac ggcctcgggc gggccgtctg aagccgcttc
     3301 agttcatcgt gggtttgttg tcgagtaaaa gcgcgctcat cagctcgtcg ccgaccaaca
     3361 cgggcagctc gctcttgttt gcccataaaa gcagcagggt cagcactttg gcggtatcta
     3421 cggtaatttc gtcgccgggg atgtgcatga gcgcgtggat gatgatttcc cgctgttcgc
     3481 agctgacggc tttttcttca atcagatact gcatcagccc catcacttcc tgcggcaggt
     3541 tgtcggtttc ttctttgctg tacacgcgca atgcgccgct gccggcgggt tcggcggaaa
     3601 attcggagct gttgagcaat acttccatca tcatcagggt gttgccgatt tccatcgtat
     3661 cgaaacccgc ttcttcaagc agcataccca agtcttcggg cggcgggcag gtatcgaaat
     3721 cttggaaatg ttcgatgagg taggcgatga cttcggtcat tcgtgttcct taatataaag
     3781 tgcgttcaag ttcggatacg ctggtatctg ccgccgggca tggcggcaac gctgccgtcc
     3841 aattccaatt ccaacagtgc ggcatacaaa tctgcggcag gcatagccaa ctgtccggca
     3901 agcacgtcgg gatgaaccgg gtcgaaaccc attttatcca gtatgccgcc gacggctgtg
     3961 ccgcctgccg ccccttcaga cggcattttt gcggcaggcg gcggggcgta tgccgtctga
     4021 acggcgcggc ggcccgtgtc gggcgtatcc ttatttatag aatatgacga agcacccgta
     4081 ttttgcaata gccccgggca ttcgttcagg atgtcgtcca aacattccgt cagtttcgct
     4141 ccgtctttaa tcagtttgtg gcagcctttg ctgtgggggt tgtctatcga accgggcacc
     4201 gccatgactt cgcgccccat ttctgccgcc aggccggcgg taatcagcga accggattcc
     4261 aaggcggcct cgaccaccag cgtaacctgc gacagcgcgg caatcaggcg gttgcggcgc
     4321 ggaaagttgc cggcatacgg ccgcgtgccg atggggaact cgctgacaat cagccctttt
     4381 tcggcgattt cataggcaag gtttttgttg gccggcggat aaatgcggtc tatgcccgtt
     4441 ccccacacgg cgacggtgcc gccttctgcc tccagtgcgc cctgatgggc ggcggtgtcg
     4501 atgcccgaag ccatacccga cacggtagga atgcctttcc cacccaacgc cctgccgaaa
     4561 tctttggcaa tccgcatcgc ctgcggcgtg gcatggcggc tgccgacgat ggcggcggaa
     4621 ggcttgtgca gcagccgcac gttgccgcgc aaaaacaaaa ccggcggcgc ggtcagcccc
     4681 tgcgtcagca tttcggggaa atcttcatcc tgaagcagca tcaggcggca tccgtcccgc
     4741 atttcccatt ccaacgccgc ttctgccgcc tgccgcgcca aagcgcgttt ttccgcattg
     4801 tgccaagcct caagcgcgtg cttatgccgc accgccgggg caacctgttc cgccggtgcg
     4861 gacaaggcat tttgcgcgct gccgaaactg cgcatcagca gcaggaaact ttccgcgccg
     4921 atatagggcg taaacgccaa ttgcagccac gcgaaacgtt cgtcctctgt cataaaatcc
     4981 atccccttac gatttcagac ggcattttct ccaaatgccg tctgaaaaca aaagcccgca
     5041 gccgggaaac cggccgtccg ggcctattct tcggtttttt cccccgcccg gcgcgaaacg
     5101 ccgatgccga gctgtttgag tttgcggtaa aggtgcgtgc gttccaaacc gaccttctgc
     5161 gccactttgc tcatattctg accttcttgg gcgatgtggt actcgaaata acgccgctcc
     5221 acctcctccc ttaattcgcg cagcggcagg ttaaagtcga acccgcacgc catatgcccc
     5281 gttccgtcgg caggcacgcc gcgccccagt gccgaagaag ccgcgccgat accgatttcc
     5341 tgcccgtccg cctccagcag cagcgttgtc acgacgtttt gcaactgctc gaaattcccc
     5401 ggccagtcgt aacgggacaa tacggcaagc gcatcgtcac tgaatgcggt aggcgtgatt
     5461 ttttggcttt ccgccacatt gcaggcaatc ccctgtatca ggaagggaat gtcttcatgc
     5521 tgcatactca ggggcggaat acggacgacc gattccgaaa aaagcccggc caatttttct
     5581 tcacatgaaa tgccgtctga acctgccgcg tagctgcacg aagcaatcac cctgacacgg
     5641 cagcgttctg ccttgccgat gataaaggta atgccggttt ggatattgcg gctgtattgg
     5701 gcgatgtcgc cgacatacaa aacgccgccc tccgccttct gcaacaattc catcggcata
     5761 tcgatcagat attcgaccct tgccggactg acccacggcg taccgttttt atggaaatag
     5821 cgcgccacgg tttcaaacgg cgaacccgcc tcgcccgtca aaaatacagg ggacgtgcgc
     5881 tttgccgccg cttcgacttc gcggttcatc tcttgaatcg cggcactgtt gcccagtttg
     5941 tcgaacaccg gtcccgtttc gatttgcgcc gcaccgtgtt tcaacgcgtt ttcgaccgca
     6001 gacagcagct tttgcaggga aatcggtttt tccaaaaaat cgatcgcgcc gattttggtc
     6061 gcttctacgg cggtatcgat gctggcatgc ccgctcatca ccaccaccgg catattgagc
     6121 tgcccgtttt tcgcccactc cttcaaaagg gtgataccgt cgcaatcggg catccaaata
     6181 tccagcagca ccattgccgg gcgcgcctga tggcgcagct tgcgcgcctc ttcggcattt
     6241 tccgccaacg cgaccgaata gccttcgtcc tgcaggattt ccgacagcag gtcgcggatg
     6301 ccgacttcgt cgtctacaat taaaatatcg ctgctacgca taagtttcta ccgtttttgg
     6361 caagatgatt ctgacacacg ccccacccgc atcctgattg ctcaggctga tgcggccgcc
     6421 gtgttctcca atgatttttt tcactacagg cagacccagt cccgttcccg ccggcttatc
     6481 cgtgacatac ggctcgaaag cattgtgcag catttccttg ccgaatccct tgccgttgtc
     6541 gcaaaccgtc aggacaatcc gtccgtcctg ccccgtttcc gattttaccc tgacttcggg
     6601 catatcggct tcttccgccg cttcggcggc atttttgaaa atattgtgca gcacctgccg
     6661 catggcggtc gtatccgccg ccatcatcag cggttcgccg gcaagttccg cctcaaaccg
     6721 gcacgggccg gcttcgtaca gggccaaaac atcgccgatt aaggcgttca aatcctgatt
     6781 ttccagtttg agcgaagggg cgcgcgcgta attgcggaat gcctcgacca tttcttttaa
     6841 cgccgccacc tgtttgatga tggtgtcggt cgaacgcgcc aggatttgcg cgtcctgatc
     6901 gtccagcttc ccgcccaatt tccacgccag ccgttcggcg gaaagctgga tgggcgtgag
     6961 cggattgcgg atttcgtgtg ccagccgctt cgccacttca ccccacgcgg cttctttttg
     7021 cgcgcgtatc agcacggtga tgtcgtcaat caccatcacc acgccgttgc cgttgtcttc
     7081 gggcaatacc gtcgccttgc ccagcaggat tttggcatcg tccggcgcgg catattccac
     7141 ctggaccggt ttgtccgtac ctgccgccgc accgatggcg gcaaacactt cggcaagcag
     7201 ggactgctgc gccgaaacgc cgtgccaacc gtgccggctg ctgccccaca ggggggcgag
     7261 cggcatcccc aaaatctgtt ccgccgcctt gttgaaggtt ttcaaacggc ctttttcgtc
     7321 aaacaccacc acaccggtag tcaacccatc caacacgcac tcgaggtagt ggcgggcggc
     7381 ttcctcgcgc cggcggttgc gttcgtctgc ttctttggcg atggaaagct gctcggtcat
     7441 atggttgaac agcttggtca aacgtccgaa ctcgtcgttg cgcaatacgg ggcgcgtctg
     7501 gctgaaatca ccctgcgcca ccgcctttgc gccctcggca agcgacagaa tgggttcgac
     7561 gaaacggcgg gcaaaataca gtgccattac cagcgcaaga aaaatcgaca gcagcgaggc
     7621 aatcagcagg gttaccagaa aaaaggtctg caaacctttt ttgctgtaac tcaattcggc
     7681 atatttcgcc cgcgcctttt caatcagaac ggcatcctgt gccacatttt cgggaatcgg
     7741 ctggcggaag aacagcgcgt aatcgcgccc gttgtgcgta cctgccgaca accatccctg
     7801 cgcgtacaat acgccgccta tgctttccaa actccgaacc gaaccggtct gctgaatctg
     7861 ttcccaatgt tctttgtcgg gaagcggctg gtcgaattgg tgcggattga tgcttttttc
     7921 gattttcccg cttgcggcat tgtacagggc aagctgggca aaaccgctgc cggcgtagtg
     7981 ttccagcaca ctgcccatat tgcccgacag ggaggcggtg ccgatgaggt ctatctgtac
     8041 gggaacggcg ttgctgacgg cattgtctgc cgccaaatcc agtgcggact tgctcaaatt
     8101 aaggctgcgt tcgagggctt cgtgggtgtc gttgccgaac cacgaattaa tcgtgccgtt
     8161 gataaactgc gcggaaatgc cgaacaggaa caagccgggc agtacggcga ccagcgtgaa
     8221 catcccggaa aggcgtttgg caatctgcga accgaacacg ccgttgcgcc tgtctttcaa
     8281 cagcaatatg acataacgtg ccaaaacggc ggacaacacc agcagcagca ttgcgctgaa
     8341 cgagactatc caccagaaat aatccgccag cgaactggtg ctgccggttg ccgccgtcaa
     8401 tccgtacagc aggacgacgg cgcatatggc tgcgatcggt aggaagcggc gcattatttg
     8461 ttcccgatga tgtttagagg tttccaaccc gaatccaaat gccagttttg agaagtcaat
     8521 gcgttgattt ggaaaggctt gggcagtttt gaagtggaca gcgtcaggcg gatttccgcc
     8581 ttggtttccc ctgcttccgc accggacaac gcgcctttgt tcaggacttt ccagttggca
     8641 accgcgccgg tcgcgcgcaa tgccgcatcc aaagtgtcgt aatcggtgga aaatgcgccg
     8701 acggtaacgc ggtagcggtt ggtcagcgga tggaaactca gtttgtagtc aatattgtcg
     8761 tcatcgccaa tcagttgccc caatttaaac cgataagaag cgattgtcgg ggcggaaagc
     8821 tgccagctta aggtaaagtt gagcggtacg ccccggcgca acgcctgttg gagctggtcg
     8881 ggcagctcgg tttggaagcg gctgctgatg gaaagccgcc cgccgtcggt tatcctcgct
     8941 tcggcgcggg tcgcggcaat gccctccgcc gccgcgtccg gcaaaacgga gagcatcggc
     9001 aacagcacaa gccattgttt aatgcttttg aataagcgcg taataaaagc catcttggtg
     9061 tttgttcggt aaaagcaccc gcgattcgat cggttcggca tcggcatggc ggttgaggaa
     9121 tttttgcagt tgcccgtcgt tttcttcgac gaacacggaa caggtggcaa gcagcattct
     9181 gccgccctgt ttcaacgcct gccaaagtgc gtccagcagg gcttcctgct ggcgggcggt
     9241 tttgagcgcg tcggtcggtc ggcgcagcca tttcacgtcg ggattgcgcc gcgccacgcc
     9301 ggaagcggtg cacggcacgt cggcaaggat tgcgtcgaaa ggctttccat cataccacgc
     9361 cgccaaatcc cgcgcatcgg cacacgccgc cgatgccgtc tgaaagccca aacgggcgat
     9421 attgtcctcc acacgcttca gacggcctgc atcgatgtcc aaggcggtaa cgcggcagtc
     9481 cgccagttcc aaaatatgcc ccgtcttgcc gcccggcgcg gcgcacgcgt ccaaaatccg
     9541 ttcgccgtct ttcgggttaa ggagatacgc cgcctgctgc gcgccgaagt cctgtaccga
     9601 caccagtcct tcggcaaaac cgggcagacg gtttaccggc acggcttctt ccaacgtaac
     9661 cgcatattcg tccaacgcct tagccgcgat accttccaca gccagttttt ccaaatagct
     9721 ttcggcgttg ccgtgccggc ggttgacgcg caacgtcatc ggcggatggg attgcagcgc
     9781 ggcggcgatg ttgtgccagt gtttcggata atggtttttc aggtaggcaa cccaccacag
     9841 cggcaggttg tatttcgcca catcgtcttt tttgcaggaa gccgccaatt tttcacgttc
     9901 gcgcaaaaag cggcgcaaaa ccgcgttggc aaacgaacgg tactgcccgc gcccgatttt
     9961 ggctatgctt tcaacagctt cgttgaccac ggcgtggggc gcgttgcgcg tgtaatgcag
    10021 ttggtacaac gccgccaaaa gcaggctttc gagctgcgga ttgtcgatgg gctttttcag
    10081 catctgcgcg agcatatgtt tcaaactgcc caaataacgc tggcagccgt aggcgatatc
    10141 ctgcaacgcg ccgttttcct gcgccgtcag ctgcggatgc gccgcgcgga tgtgcgccaa
    10201 cacgtcctga aggttgcgcc cttcggcaac cgccgcaatg ctgtcggcgg caagtttttg
    10261 ggcaagtgcc atactcatat tatgtgattc gctttcttca actgatggtt tcagacggca
    10321 ttcccgtttg cggatcggaa tgccgtctga agggatttac agcttcgccc ctgcttcaat
    10381 agaccgtccc gccgcaaacg ccgcaatatt catacgcctg ccgccggcag gctgcaattc
    10441 ggtaatcttc agcgcgcttt cgccgcaggc aacgaccaaa ccgtccgccg aacaggacaa
    10501 cacttcgcct gccgtacctt gttgcgccac cacttcggca cgccggattt tcatcggctt
    10561 gccctgatat tcgacccacg cggcaggcac ggggttgaag gcgcggattt tgcgttcgat
    10621 aatgtccgcg ctttcgctcc aatcgatacg cgcttcttct ttgcttagtt tttgggcgta
    10681 agttacgcct tcttcgggct gtttgaccgc gttcagacgg cctttgcttt gaagctgttg
    10741 caaatcggca acaaccgccg ccgcaccgat ttccatcagc gcgtcgtgga cttcgttggc
    10801 agtatcggta ggttggatgg cgtagcggtg ttcgctgacc acatcgccgg tgtccaaacc
    10861 gatgtccatc tgcataatgc acacgccggt ttcggcatcg ccggcctcaa tcgcgcgctg
    10921 aatcggcgcc gcgccgcgcc aacggggcag cagcgaagcg tggatattga ggcagccgtg
    10981 tttcggtgta tccaacactt cctgcggcag aatcaagccg tacgcggcaa ccaccattac
    11041 gtccgcctcg acctctttga gcatttgcag ggcttcggcg ttgttgcgca gtttttcggg
    11101 ctgtgccacg cgcaaaccga gttccagcgc ggcttgtttg acaggcgggg cggtcagttg
    11161 cataccgcgc cctttcggac ggtcgggctg ggtcagcacc agcgggattt caaaaccggc
    11221 ggcggcaacg gcttttaagg cggcggcggc aaaatcgggc gtgccggcaa agatgacttt
    11281 catagcgtgt tcctgcgtgt gcgtaaagtt gtcggacaag gctgtgccgt ctgaaagcct
    11341 tgcagcgttc agacggcatg acgaagcgaa tcaaatcgta tgtttctgac gttttttcag
    11401 tttggtctta atccgcccct gcttgagttg ggaaaggcgt tcgacaaaca cgatgcccat
    11461 caggtgatct aactcgtgct gcacgcaaat cgccagcagc ccgtccgcct ccagcgtgaa
    11521 tttttcgcct ttttcgttca aagcctcgac cttgacgcgt tcggcgcggg taacggcgtc
    11581 gtaaatgccc ggtacggaca ggcagccctc ttcgtaagtg gtttcgccgt ctttttcaac
    11641 gatgacgggg ttgatgaaca cgcgcggttc gctgcggtct tcggtcaaat ccatcacgac
    11701 cacgcgttcg tgcacatcga cctgcgtcgc cgccagcccg atgccgcgcg attcgtacat
    11761 cgtttcaaac atatcggcaa ccagcttccg gatgcgctcg tcaacttgtt cgacaggctt
    11821 tgccaccgtg tgcagacgct cgtcgggata ttgcaagata ttcagtaaag ccataatttt
    11881 ctctttcctt cggcggatac gccgcctgtt tttcataatt accgtgatag tgcggataat
    11941 tcgatgttaa aatgcggaac atttgttatc acgcggcaca tcccgagggg ctgccgccgc
    12001 tttttccaac ccaatcaagc aagacaaacc gaaatttatc agataagggg aacggttatg
    12061 caacgtcgta ttataaccct gctctgcgcg gcaggtatgg cattctcaac acaaactttg
    12121 gcggcaaatc tggaggtgcg cccggatgcg ccgcaacgct acacggtcaa acagggcgac
    12181 accctgtggg gtatttcggg caaatacctg tacagcccgt ggcaatggtg ccgcctgtgg
    12241 ggcgcgaacc gggatcaaat ccacaatccc gacctgattt atccgggcca ggtattggtg
    12301 ttgcgctacg ttggcggcga accgcgcctt gatttggaac agacagacgg catccccgtc
    12361 gtcaaaatca gtccggataa ggaagtgtcc ggatacggta ttcccgccat cgatgtcaac
    12421 ttctaccgcg tctttatgca gcacccgcaa atcgtttccc gcaaagaaac cgctgccgcg
    12481 ccgcgcctgc tctcgggccc ggaaggcagg ctgctgtaca ccaaaggcgc cagggtttac
    12541 accaaaggcc tgaaagagcc gggccgttat ctgacctacc gaatcaataa aaacatcacc
    12601 gatccggata cgggtaaatt cctcggacaa gaagtcgcat tcagcggcat cgtgcgctcc
    12661 ctcgactata ccgactccgc cctcgaacaa cgctcgaaac aggctgaaga acggctgaaa
    12721 gacaacgaat actacacccg cacccatcct ttgattaccc cggtgcgcac ccgttcgata
    12781 cagccgctgg tggtcgaaac cgccatttcc gaaatacagc agggcgacta cctgatgaaa
    12841 atgccggaag acaccgaccg cttcaatatg gtgccgcacg aaccgtcccg cccggttcag
    12901 gcaaaaatcg tttccgtgtt tgaaggcgtg ggggtcggcg gacagttcaa aaccattacg
    12961 atagacaaag gcggggacga cggtttggac aaaggcgcag ttttaagcct ttacaaacgc
    13021 aagaaaacga tgcaggtcaa cctctccaac aatttgaccg aagaacctaa aagcagggat
    13081 accgtcgagc tgatttcgac acctgccgaa gaagtgggct tggcaatggt ttaccacact
    13141 gcgccgaaac tggcttacgc cattattttg gaaaacatct ccgatatttc cgaaggcgac
    13201 accgccgcca atccgggacg ggatttggac aatatgccgg atcagggccg cgcccgcgtc
    13261 gattccgacc ctttccaata acccctcggc aacaatgccg tctgaagcat tcagacggca
    13321 tttttattta ggctgtgttt tcatatcccg ttgatgcaaa accctgattt agtcggacaa
    13381 aatgcctttt atccgatgga atcggtttcc gcccgaacgg aaatcacagc ggctgtctcc
    13441 cttgcagaca gatatagcga attaacaaaa accggtacgg cgttgccccg ccccggctca
    13501 aagggaacga ttccctaagg tgtccaagca ccgggcgaat cggttccgta ctatttgtac
    13561 tgtctgcggc ccgccgcctt gtcctgattt ttgttaatct actataaaac caaccgggta
    13621 tccaaaccca atcttgccgc tttgccgccg cgccacccgt gcgctcccca tacatcggga
    13681 caccttcaat aaaatatctg cttaggcttt tccggcgttt tcgagatttc cctcaacgcc
    13741 tgattgagcg cgcccaaatc caacgccgtt tccgaacggc aggcatcgcc gaagatgttt
    13801 tttatattcc gccgtcagtt tcagtatccc tttgaacgcg cgcccttcca aagccgccga
    13861 caactcgaaa atatcggcga acacgcgccc ataggtttcg cccgaggcga ccgcgtccgt
    13921 ccccaatgcg aacatatcca tttttgccaa ctcggcggca atcgtgcgcg cgtcttcgtc
    13981 gtcgggcagc acttccgcct tgaacctgac ctgttccgcc atgcctttgc cgtggttttt
    14041 gaccagcagg gcgagcattt gcggcttgtg ttccaagtgt tgcaaagaag cgtgcaggac
    14101 gggggtatgc tttcgttctg tatcattttt tcgcggtaga acttttctgc cttagccttt
    14161 gaacgcaccg acacgaacgc ccacgccagc cacaccagcg cgacggcgga caatgcggac
    14221 agaagcggag gcagctcttc agtataggct ttctgattga accagatttg cgcggtcaaa
    14281 aaaccggcaa agaaaatcaa aacataaagt atcggtgcga aacgactttg gacagaagcg
    14341 tgcatcatca ttccatttca gattggcaaa acaggcggca ggcaaaattc cgccgccggg
    14401 ctgcgggaat ccgtcaggct tccgccaccg aatcccggtt gtaggttttt tcgcagtagt
    14461 ggcatttcag ccgcgtctgc ccgttgtgtt tcttaacata aaaccggctt ttgaccggct
    14521 cgccgtggcc ggcgcaattg ggattcgggc agcggaacac ttcggcgatt tcgtcgggca
    14581 gggtcaaatg ccgcttctgt acgaccttga aattgtcgat ggtgttgacc accgcttcgg
    14641 gggcgaacag ggcgaggcgg tcggcggctt tatcgtccaa gcacacgcct ttgattttga
    14701 tgatgtcttt gctgccctgg gttttgctgg gcaggttgaa gcccacggtt accgcgttgc
    14761 cgtagtgcaa aagtttgaac tggcgcagga tggtcagccc cctgccggcg ggaatatggt
    14821 cgataaccgt gcctttttcg atggcttcga cactgagttt ttgcgcttcc atatcggttc
    14881 ctcacacttc ttcgttcaac accagcgaca atatcgccat acgcgcataa acgccgttgg
    14941 tcgcctgctc gaaataatag gcgtgcggcg cggcatcgac atcggggtgg atttcgtccg
    15001 tgcgcggcag ggggtgcagc acgcgcaggt tcggtttggc gcgggcgagc atagacgctt
    15061 cgaggttgaa tttgccttgg attttggcaa attcctgttc gtcgaaacgt tcgcgctgga
    15121 cgcgggtcat atacaggatg tccgcccatt ccgccgcctc ttccaaactg ccgagggcgc
    15181 ggtatcggca gtcggcttct tccaactctt cggtaatgta gtcgggcatc gcgaggctgg
    15241 gcggcgagac gaaggcaaat tcgcagcccc agcgtttcaa cgcctgacac agcgaatgca
    15301 cggtgcgccc gtatttcaag tcgcccgcca tggcgatttt aagcttgtcc aaacgcccct
    15361 gcgtttcata aatcgttacc aggtccaaca aggtttggct ggggtgttgg ttcgtgccgt
    15421 cgccggcgtt gatgacgggg acgcgcgaaa actccgccgc cacgcgcgcc gcgccgtctt
    15481 tgggatggcg ttggacaatc gcgtcggtgt agccggaaat gatacgggcg gtatcggcaa
    15541 gcgtttcgcc ttttttggca ctggtattcg cgccgtccga aaagccgatg accttaccgc
    15601 ccaaacgctg caccgccgtt tcaaacgaca gcctcgtgcg cgtggacggc tcgaaaaagc
    15661 acgaaccgat gagtttgcct tccaacaaat cgccgcgcgg atgcgccttc agcttcaatg
    15721 ccgtctgaag caggtattcc aactgttcgc gcgacaaatc cgaaatggag atgatgtgct
    15781 gtctgtaaag cggattaggc atttttgccc cccttccgta aaaaacccgc gtcaggcggg
    15841 catccggtta tacgtccacg ccgcccgtat gtttgcggaa cagcttcgca aaagcggcga
    15901 cacggcgcgt attatcgcac atttgcctcc acaaagtttt accgcacgaa tcctgtattt
    15961 ttcagacggc atatccaaac acgcccgcat aagcataagg tataatcgcg ccagacattt
    16021 cttttcagga agccgccatg ttaatctgca acccctacga agtcgtcatc cacggcacaa
    16081 cgagttccgg caagattttc cgccccggcg actgggcgga acgcctgtgc ggcatcctgt
    16141 cctcgttcac caaagacaac cgcctctcct attcgaaatg ggtgcgcccc atgctggtgg
    16201 acaacatccg ctgcgtcgcc gtcgataaaa aactggaaac cgacaatccg cagatgttcc
    16261 gcttcctgat ggactttgcc gccgacaacg acctgcgcgt catcgactgc aaagccctgc
    16321 tcgaagaacg cgaacagggc ggacaaaacg accctgccaa cgaacgcgtc ctgctggcac
    16381 aggcaatcga agaaaaacac gccgccgaga aaacacagga acagaccgcc tcgggcgcat
    16441 cctacgtttt acgcgaaatc ggcgcggacg acaccgccac cgcctttgca gccttgagcg
    16501 ttttgcgttc cgccctgacc gacatcggcc gctttaccga acagatcaac aaaacccaac
    16561 gcccccaagg ctaccgcctg ctgggcattt ttgaagaagg caaacacaac gccgtcgctg
    16621 tctgcggctt ccgcgaagcc tgcaccctcg ccggcggccg ccacatccac atcgacgaca
    16681 tcgttaccct gccgcaaagc cgccgcaaag gctacgcctc gcgccttttg gaagaagtcc
    16741 gcaaaatcag cgcggaaaca ggggttacca aaatccacct taacgtccac gtcaaccacg
    16801 accgtaccga cgcgcaccgc ctgtatttca aaaacggttt tgaaatctgc gcctaccact
    16861 tccgttgcga ccccaaatga aaatactcct cctcatctgc accctgtcgg cacccgccgc
    16921 ctgaaccacc ccgtccggac aaggcggcac tcaggtttac ggcgaaatca aagcccgaga
    16981 cctttgcgtt gcctctccta tcctgctttc tgtcttgcct gctctcgttg agccaagcgt
    17041 tcttgcaagc tcgcttgcgc gttggcaagc attgcactct atcggctttc ttttcctatt
    17101 gcggctggtg gttcaggctc gcgttgtacg ctttgcacta aagtacaccg tgaatcgatg
    17161 ctcgctattt atagtggatt aacaaaaacc ggtacggcgt tgccccgcct tagctcaaag
    17221 agaacgattc tctaaggtgc tgaagcacca agtgaaccgg ttccgtacta tttgtactgt
    17281 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataattgtt aatcactttt
    17341 aatcagctaa ctaatttctg taatagaatt agttagctat ttattaatat aggttgcatg
    17401 attatgggtg tattaatttt ttttctaatc gttccaattt tgggatttat ttgcgctacg
    17461 ataaattatt ttattattaa taaatttaaa cttccaaaat acatggcgta tttactccca
    17521 tcattatcta ttttattcat atttattcat gcaataaaat tgcatatgat tttatttttt
    17581 tatgtttcat gcgtttattc cgcgtataca tattatgata aaaaatcttt ataaattgat
    17641 aaattatgcg gctttgattg gtttcgcccc aaatatagtt tttgcttggc ctatgtttgt
    17701 tgacccccgc ttcttaccaa aacatccatg tacggagttg gaaagcacgg cgagattccg
    17761 gcatagtaaa acaagatttg gatttttctt gcggcgcggc ttcgattgcc acgttactga
    17821 ataattttta tggcagacat tattctgaag cggaaatctt agacaaaatg gataaaaccc
    17881 aaatgcgtgc ttcttttgat gatatgcaac gcataatgcc cgaactgggt tttgaagcac
    17941 aaggttatgc tttgccattt gaacagttgg tacaactaaa aattcctgta attgtgtatt
    18001 taaaataccg taaaaacaac catttttcgg tattgaacgg tattaacgga gaaactgttt
    18061 tgctggctga tccatcgctg gggcacgtct caacgagcaa atcacaattc ttgagcgcat
    18121 ggaaaacacg tgatggcgaa atggaaggaa aaattttagc catcgtgcca aaaaatactg
    18181 attttgttag aaatcagatg ttttttaata aaaatcccgt tcgtcaaaca cgttttacgg
    18241 tagaacaaat ccaaatgcgg caaaaacgat aataaaccga tttttgccca tttattttat
    18301 atagtggatt aacaaaaatc aggacaaggc ggcgggccgc agacggtaca aatagtacgg
    18361 aaccggttcg cccggtgctt gggcgcctta gggaaccgtt ccctttgggc cggggcgggg
    18421 caacgccgta ccggtttttg ttaatccgct ataaaagcaa agaaaccgcc attagcacta
    18481 caccccccca aactgcggct gccaaacaca tcggctgctt ctcgacgcac ccgctgaaaa
    18541 atgcgcccta tccgtccgcc ttgcccgcac cccctgcctt tgccgccctg cccgatgccg
    18601 tctgaagcct gaaccgcccc accagcccga tgacgcacga caccgccgga aagggcatac
    18661 acaaagccgt cagcacataa cgcggattcc ccgtattgac cacgtccgcg cccttcagca
    18721 gcagcggcag cgcgttcgcc cactcccaca gcgaaggcgc gctgaacgcc aataccgcca
    18781 ccagccccaa catccggaac accccgattt ccaagacctg ccgccgccgc accgaacggt
    18841 tgagcagaaa cacaaccgcc aacccgaaac agaccaccgc cgccataccg ccgttggcga
    18901 caatctgcaa actgttgaac gcaatatcgg gcgtaaccgc caacacgcct tccgaatccg
    18961 gcacggcacg ctgcaaatcc aacccctgca acacattcaa cacaaaaccg tagatcaaat
    19021 caaaaaacac cacgcctatc ggcaaagcac tcaaaacccg ccacatcttc ctgtccgacc
    19081 aaccgttcaa aaccttcatt ttacccgacc gcgcaagccg ccgaacaaaa aataaattta
    19141 gcctaaatca taaaaccgtg taattttgca ccaaaaaagg cggtttgcag gtaaaatatc
    19201 aaaccattaa tttaaatcat atttttacag aatattccgc cgcgttcacc aaatggacat
    19261 caaaccggtt ccaaatttct gtaattttgc aacaaaatac cgcaaaacac ccgattgaga
    19321 ccgaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca gccacaacac
    19381 caaaatcgtc gccacccttg ggccgggcag caacaacgtc gaactgttgg aagacatgat
    19441 ccgcgtcggc ggcctgaacg tcgtccgctt caacttcagc cacggcacgc ccgaattcca
    19501 tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc gccggacagg aaatcgccat
    19561 cattgccgat ctgcagggcc cgaaaatccg cgtgggcaaa atcgccggcg gcggcatcga
    19621 attgaacaaa ggcgaaacat tggtactcga tgccgcgctc gaaggcgaag gcacgcgcga
    19681 ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg atgtcttgtg
    19741 gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca ggattatcac
    19801 aagggtggaa aacagccaca tcctgaaaag caacaaaggc atcaacaaac gcggcggcgg
    19861 tttgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg cgattgccat
    19921 cggttgcgac tacctcgcca tcagctttgt gaaatccgcc gaagacctgc acatcgcgcg
    19981 cgccaaagtc gaagaggaaa tgaagggcag cactgccgtc cgccccggtt tggtttccaa
    20041 aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atccttgccg gcgacggcat
    20101 tatggttgcg cgcggcgact tggcggtcga agtcggacac gccgccgtcc ccgccctgca
    20161 aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg cgacgcaaat
    20221 gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg atgtggcaaa
    20281 cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg tcggcgcgta
    20341 tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa aagagcagga
    20401 ttcgctcaac ggcgttgccg aacagacgga gtatcccgaa gccgtcagca ccaacttagc
    20461 gattgccggc ggcgcggtca gcgtggcgcg cgcggttcac gccaaagcca tcgtcgccct
    20521 gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aatattaccc tgccgatttt
    20581 cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg gcgtgcgccc
    20641 gctgattttg gcaaccagca ccgaccacga cacggcgttg aacgaagtcg aaacgatgct
    20701 ggtggaacac aacatcctgc attccggcga ccagtacatc atcaccagcg gctcgcaaat
    20761 gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat ccgccctgag
    20821 tggaaaatgc cgtctaaagc cgatactgtc gggcttcaga cggcatttta tttgcattcc
    20881 gtcaggtaaa accgaaagaa ttgtgcaaat ccggcaacat cggataaaat cgagtgccta
    20941 tactaaagcg aaacaaggca tttccgactg ccttttttat ttgtccaccg tccgcctttt
    21001 tacggaaacc gaaatgaccc ctttcacact gaaaaaaccg tcctgctgct cggcactgcc
    21061 tttgccgccg catctgtcca cgcatccggc taccacttcg gcacacagtc ggtcaacgcg
    21121 caaagcacgg caaatgccgc cgacgcgtcg accatcttct acaatcccgc cggcctgacc
    21181 aaactcgaca gcagccagat ttccgtcaac gccaacatcg tgctgcccag cattcattat
    21241 gaagcagatt ccgccaccga ctttaccggg cttcccgtcc aaggttctaa aaacggcaaa
    21301 atcaccaaaa ccacggtcgc accccacatt tacggcgcat acaaagtcaa cgacaatctg
    21361 accgtgggct tgggcgtgta cgtccccttc ggctctgcca ccgaatacga aaaagattcc
    21421 gtgttgcgcc acaacatcaa caaactcggt ctgaccagca tcgccgtcga acctgtcgcc
    21481 gcgtggaaac tcaacgaacg ccattccttc ggcgcaggca tcatcgccca acataattcc
    21541 gccgaactgc gcaaatatgc cgactgagga atcccaaaaa aagcgcaaat gctgcaagca
    21601 acaccttcta atcctactgc cgctgctcaa atcaaggccg acggacacgc cgatgtcaaa
    21661 ggcagcgatt ggggcgtcgg ctaccaactg gcgtggatgt gggacatcaa cgaccgcgcg
    21721 cgcgtgggcg tgaactaccg ttccaaagtt tcacacacgc tcaaaggcga tgccgaatgg
    21781 gcggcagacg gcgcggcggc gaaacaacag tggaatgaca atatgctcac accgctcggt
    21841 tacacggcga atgaaaaagc cagtgtcaaa atcgtaacgc ctgagtcttt gtccgtacac
    21901 ggcatgtaca aagtgtccga caaagccgac ctgttcggcg acgtaacttg gacgcgccac
    21961 agccgcttca ataaggcgga actgtttttt gaaaaagaaa aaaatattgc taatggcaaa
    22021 aaatccgacc gcaccaccat cacccccaac tggcgcaaca cctacaaagt cggcttgggc
    22081 ggttcttatc aaatcagcga accgctgcaa ctgcgcgtcg gcatcgcttt tgacaaaccg
    22141 cccgtccgca acgccgacta ccgcatgaac agcctgcccg acggcaaccg catctggttc
    22201 tccgccggca tgaaatacca tatcggcaaa aaccacgtcg tcgatgccgc ctacacccac
    22261 atccacatca acgacaccag ctaccgcacg gcgaaggcaa gcggcaacga tgtggacagc
    22321 aaaggtgcgt cttgcgcacg tttcaaaaac cacgccgaca tcatcggcct gcaatacacc
    22381 tacaaattca aataaacgtt acaccgtttg aatataaaaa tgccgtctga aacagatttc
    22441 cgtttcagac ggcattttta tccggcagcc gatacgccta tctcggtatt cgggagaata
    22501 tatacagaac ctccttcata tcaaaatggt agggacgctc gggcaacagg acggcggcag
    22561 gcagcttggt gatgccgttc aaaccgcgcg ggaacagcgc aatccgctct ttgccctttt
    22621 ccgcataatc tgctttgcgg ttgtggatgg aaactacttc gcgatggcgg taaagcagcg
    22681 tgttcatttc gatataggct tcgttgtgga tggctgccga ctgtttctgc aacacgtcaa
    22741 tctgcctgtt gagcttgtcg ttggctttca tcagcctgtt gaagaacgac ggttgctcct
    22801 gcagtttggc ggtacaggct tcgatacttt ccgataccgg cccgaactgt tcgaggtatt
    22861 tttccagaca gttttccaag tcggtcaggc agccggccat cttgcgggaa aattcttctt
    22921 caggctttcc ctgccgaatg gacaatatag tggattaaca aaaatcagga caaggcgacg
    22981 aagccgcaga cagtacagat agtacggcaa ggcgaggcaa cgccgtaccg gtttttgtta
    23041 atccactata tttgggaggg gcgtaacccc ttccgaatca ggacggcaca tagggcgacg
    23101 ctttatgtgc cgtcctgtgt gttgaaacat cagcatagga aacacaggaa aagccggctg
    23161 ttttgcgcct gcgtaccgta ttggtcaaaa tgccgtctga aaccgacaac agggtttcag
    23221 acggcatttc cgtcaatttt ccctttatct gccgaacagg ttggcgatat tttccatctg
    23281 ttcttcggta aaaccgtttt cgcccaatct ggcggcaact gcccattcgc ccgacaaacc
    23341 gtggtccgca ccgagctgcc gccatttttc gactttggct tcggctgtgg ttttttcggg
    23401 cagcggtttg acgggaatcc tgcttttggg gacggtattc atcggtttgc cctaatcgat
    23461 ggaatagccg cgaagatact gtatccgttc gcgcgtcatc cgcgtgtcgc attggagctt
    23521 gaggtattcg gcgtattcct gccggtctgc ctgcgcggcg gcttgtcggc agttgctgat
    23581 tttttcctgc gcccacttgc gctgttcgcc gaccaactct ttttgcacgt cggtatcgag
    23641 tcctccccaa agtttggtaa tttcggattc cgcacgttgg ttttgtacgc gcgcctcttc
    23701 cacttcgccc cgtgatacgg taacggtatc ggcacgctcg acgtcgtcgg gatgcaggat
    23761 ttcgggttcg ggcgcgcctt ctgcggcttg gggtacgccc gcatcgccgc cggcggcatt
    23821 gtgttccaaa atgtcttcgg gggtgggttt ggacggttct tcttcacggg ctttgccgct
    23881 caaaaccctg accgcgtctt cttttgtcac cgccttgccg tctatcatca cgatgctctt
    23941 cacgccgtaa ggcagcaacg cggcagacag cgtttgcgcc gccataccga ccgtgttgtc
    24001 gataaatgcc gtccgagcgt ctttggcggg caggaagcgg acggctgccg tcaatacgcc
    24061 gtctttaaac tcgacattgc cgcccgtctt ctgctgcacg atgtctgcca aagacgtttc
    24121 cccatacagc agggggctgt ttgcctcggc atcggcaagc gtttcagacg gcacggtaat
    24181 gttcaaatcg gcgatacaga acgtgcgccc gccttcctgc gtttccgaag cgtgttccaa
    24241 agaaaacgcc aaaccgtagg cggcggcgat aattttgtcg gcatcgacaa actgcctgcc
    24301 gtcttcgcgc gcgaaagaac gcgcttcctg cgtgagcgtt tcctgaatac tgccgcgtat
    24361 gtcctgcaac acggcggggt tggcgcattc caacgccttg ggcggttctt ccctgccgca
    24421 cgcggcaagc agcagggcaa acggcagcgc aatgagtttc cgatacatga tgttccttcc
    24481 aaacggtgca gaccgatggc ggctatcata gcaaaagcac ctgcaggcgg taaaccttgc
    24541 aggcgctttg tcggaaaggc ggaaaatcag cctttaaaga aattcacaaa accggtgaga
    24601 atcgcggcat taatcaaatc gacaaagaac gcgccgacca taggcacaat caaaaacgcc
    24661 ttatgtgacg cgccgaaagt atgcgtgacg gactgcatat ttgccaccgc cgtcggcgtt
    24721 gcgcccaaac cgaaaccgca gtggccggca gccaatactg cggcatcata gtcgcgcccc
    24781 ataaagacat aggtaacaaa agtcgcgtac aaaaccatca ctgcggtttg tactgcaaga
    24841 atcacggtta cagaccccgc caaaccggtc agctcccaca gtttcaaatt cagcaacgcc
    24901 attgccaaga aaagcgaaag cgaagcattg ccgaacacat cgatggcacg gtcgaacata
    24961 ttgaccttga atgcggcggt aaggatgttg cggataacca cgccgccaaa cagacaccac
    25021 acgaatttgg gcaggtcgaa caggtattct ttgtcgaaac cgtccataat ctcggcaaac
    25081 gccagacacg cggcaaacat ggcaagcgtt tcaacggcag attccgccgt aatcaggcgg
    25141 gtgcgttttg cctgctcgaa cacgtcgtcc gcgttgtcgt cctgatcctg ttttgtgttt
    25201 tcaaccggtt tgcggcccat tttgttgatc aggcggcgcg caaccggccc gccgatcagg
    25261 ccgccgaaca ccagcccgaa agtagccgaa gcaataccca aaccggttgc gccgaccaag
    25321 ccgtattgcg tttcaaaatt aggtccccac gcacctgacg tgccgtgtcc gcccgtcagc
    25381 gacaccgaac cggtaatcag accgatgagc gggtccaaac ccaaagccgt agccagtccg
    25441 accccgacaa agttttgcac caagataaat ccgcccacaa tcgcggtaaa aaccaccagc
    25501 ggcaaaccgc ccgccttcaa acgggaaaaa tccgcgctca agccgatgga cgtgaagaaa
    25561 atcagcataa acgcattttg cagcggtttc tcaaatttga agctcacgcc gtacgcctcg
    25621 tgcagcgcaa acaggataat cgcggcaatc agcccgcccg ccacgggttc ggggatgtta
    25681 aaatcacgca agattttgat tttcttaacc aaaaccttgc cgaccaacaa aaccaaagtg
    25741 gcggcaatca aggtgtaata actgttaaac gcccattcca tatcgtttga cctcctaaaa
    25801 tgtttttctt tatatccgcc cgaaggggac gcaagcgcga agatattagg ggaatagtta
    25861 aggcttgtca aagaatcgcc gttttttata gtggattaaa tttaaaccag tacatcgttg
    25921 actcgccttg ccgtactatc tgtactgtct gtggcttcgt cgccttgtcc tgatttaaat
    25981 ttaatccact atacaactat tttttacaaa caaaatgccg tctgaaactc cgttcagacg
    26041 gcatcatggg aatacacata tttgaaataa cagaaaaagc gggggcaaaa cccgccgctg
    26101 catctgtcgc tgtacatccc tgctgtcgga cttaccgctt caacttcgca aacgcatccg
    26161 ccatcgccga attggttggg acacgctcgt tgcgttgtgg tttgcggccg gctgtccgtt
    26221 cgcggctgcg gtttttccag ctcggacaga atgtccagaa tggtcggcag tccgaagtgt
    26281 tcgtcggtga agtcggacgc tttgatttgc ttcacgcgct cgcggttaac tgcctaaata
    26341 tgaaattctt ggtcttcttg tagcataagt tctcgatgat atgccaagta agatttacgt
    26401 ttgtcgttga gcagattgtt atctagcttc acatcacacc aacgtcgcca aacatcatct
    26461 gataaccgct tagatttcac catatttcct tcatcatcaa acgaaatata tttcaaatca
    26521 aataggctat ccaaggtacg acttaatagc aagccgttat ttgggtcgta tgcttcggta
    26581 tcatccgaca ggatgaaagg cttaatatga ctggcaatca atacaggata ggcaagtttt
    26641 tccagcatac atttcacatt accgtaatgt tcttcacttt cttcttgaag ttggtttttg
    26701 tataggcgat gtaaataagg gtcgcgtttc cttacagtaa tttcatccaa attaccaaaa
    26761 atacgttggg catcctcagc aaaatataag tcatcaccta ctcgttttaa gtcgtctaat
    26821 ttgtctaata ggttccataa ataacttatt tgattgtatt ttcgttcaat aaatcctgat
    26881 tctattccct gttgaaaata atcattcaat tctgtttcgg ttagataatc ttgttgaaag
    26941 gtttttaagt ctactaacat catggcggca atttcttttt tatttagctt gccttgtgga
    27001 tgctcgacca gcgttttaat caggaaattt atttgtcgaa tattagagtt ttcagttact
    27061 gaacgctgaa agccagagtg cgtatatacg attttggaaa gtagggtttg tcgttttcgg
    27121 ttagtacgag cttgtacata atctcgtgat aaaggtgtat agcttgataa aaacggttct
    27181 ataaatccca tttttacaaa ttgattaata gctttacgaa ccgaggccaa attcatggcc
    27241 ttgcttgttc tctgcgaagc cataacggaa tcttgggcaa tgttttgctg caatgcttca
    27301 tatagttcgt tgctgtatgg ctggtcttga ttcgcatcaa taaagtcaag acaaatattg
    27361 agtgcagtac aaaatttttg atcgttataa tttgtaaagg cattggtaaa cgcccaataa
    27421 tcttcataaa tttggctgtt tggctgatta ttactcatct ctgtctccaa gaatattcga
    27481 taccaatatt ctgtttttgt atttaagtaa tatctatcat tcatgtattt tctactaaaa
    27541 acagaatttc cttatggctt tgcttctttt cttttcctgt aaccttataa acgtgaggcg
    27601 tttccaagat ttgtacattt cgcccatttt tacctaacat ttcttctaat tcatctcggt
    27661 ttggatatga agcattgttg tagcttaaca gccaatattt aaatggtttc aaatgattga
    27721 ttaccctttt aaataattcg acagcctgct ttttatccat gaaatgattg gcaaatggtt
    27781 tgggtatgga tgatgtaatg taactgtcca ataatccgta aaagccaaaa taattgttca
    27841 tcgtgcctgt gtaaggtggg tctaaataga cggcatcagc ttgtatatta ggtaataaat
    27901 caaatatatc ttgattaaag gcgtgatgcc gccagccatt attgaaaaca gcttggttat
    27961 agtcatccaa attttgcaga aaatgacttt gaaaactttc attgtggtag gcgcgtcgac
    28021 ggccatattt agcataactg tattcttcat cacgcagttg tttaactttt tcccaaggaa
    28081 ttgtgaatcg tgaatagggc attttacgta tcatggcgcg acgcattaaa gtaaacgcca
    28141 aagcacgttt atactggtta ttcagcttcc ctatattttt acagtacaaa tcaagttgtt
    28201 ggcattcatc gtgaaaatag aatttttcgg aataacgctg actcataaaa ccggcatgcg
    28261 ggctgcctga aaaaatcatt gcgacatcat cgtcatttaa tgtttcatgg ttgttttcta
    28321 ttaatgctaa agcaatttgg taattaattg ccaaaatatc gttagtaatc acacgatagc
    28381 cgcgtttttt ggcttcgtag gcaaaggaac agcctccact aaatacatct gcaactgtat
    28441 ctacatcaga cggaagctgg tcacaaatcc aggaagctat tttctcttta ttaccgatat
    28501 agttaatttt cggatatttc ttattcattt tcactcattt ttaaaatact ttcagcaata
    28561 gcttttgcca ataaaggcgg cacggcgtta ccaacctgct gctgctgggc aattttgctg
    28621 ccgcaaaaaa taaaattatc agggaaagat tgtaaggcag ctaattcacg aacggttaaa
    28681 gcccgattct gttcatagtg aaaaactttg cgcatatctc ctgtaataca aacggctggt
    28741 ttgttgctgt tgtaacggat gtatttacgg atatcacctg ttttcggacg taatggttca
    28801 ggaatatcgt tacggttacc tccattttta acaaatgcca ttttttctaa catttgtgcc
    28861 gaatgattca tagcttcatg atttgcaacg tgtggattgc tttcgccagc agccagtttt
    28921 ggaaaatgtc ctattgctga tccaacagtc tgatgggaaa tctgcaaagg ttcgggaaag
    28981 gaaattttgc ctttatccct cctcccgata aatatcactc ggctacgtat ctgaggaaca
    29041 ccgaaatcgg ctgcactcag tatcttacat tccaccgaat aaccgatatt ctgaaatgct
    29101 tgaataatct caatacgtgt tttacctgaa ttgtgtgtat agagtcgcgc tacattttcc
    29161 ataacaaaaa aatatggttg gacaatttta actattcgga caaactcttt aaataaatgg
    29221 ttgcgtgggt catctgtaaa tgtccgtcca atctttcctg ccatactaaa accttgacaa
    29281 ggtggtcctc caataaccaa atcaactgat tgtccgttaa gacaattggt taaatcttgt
    29341 tcggttagtg tggttaaatc tttttgcagt aattgatgac gggggaagtt ggtacggtaa
    29401 gtctgacaat aatcagactc catttcaaca gaaagcaatt gttggaatcc ggcttgttca
    29461 aaacccaagg ataggcctcc tgctcctgag aaaaggtcaa tataggtaag tggtgtgtgc
    29521 ataagtcaaa atccataaac tcttcgatta ttttaccgct tcagcttcgc aaacgcatcc
    29581 gccatcgccg aattggtcgg ggcgcggtcg ttgcgttggg gtttgcggcc ggctgtccgt
    29641 tcgcggctgc ggttttcaga cggcatttta tgttttgcgc cgcccggttc gtcatccaag
    29701 cgcatggtca gcgcgatgcg tttgcgtgcg gcatcgactt ccagcacttt cactttcacc
    29761 acgtcgccgg ctttcaccac ttcgcgcggg tcttggacga acttattaga cagggcggag
    29821 atgtgcacca agccgtcctg atggacgccg atgtccacga acgcgccgaa gttggcgacg
    29881 ttggagacca cgccctcgag tatcatgccg acttgcaagt cgctgatttc gtggatgcct
    29941 tcggcaaacg atgccgtctg aaacgcaccg cgcgggtcgc ggccgggttt ttccagctcg
    30001 gacaggatgt ccaaaatagt cggcagaccg aagcgttcgt cggtgaagtc ggatgctttg
    30061 atttgcttca cacgctcgcg gttgccgatg agttcggcgg cggtaatgcc ttgttgcgcc
    30121 agcattttgg cgacgacggg ataggcttcg gggtggacgg tgctcgcgtc cagcggctct
    30181 ttgccgccgt taatccgcaa aaagcctgct gcttgctcga aggttttttc gcccaagcgc
    30241 ggtactttta gcaatttttt tcggctgtcg aacgcgccgt tttcatcacg gtaggcgacg
    30301 atgttttggg caagggtttg attcaagccg gaaatccgcg ccaagagcgg ggcggaggcg
    30361 gtgttcgcgt ccacgccgac ggcgttcacg cagtcttcga ccaccgcgtc cagcgatttg
    30421 gcgagccggc tttggttcac gtcgtgctga tactgcccca cgccgatgga tttggggtcg
    30481 attttgacca actcggcaag cgggtcttgc agcctgcggg cgatggacac tgcgccgcgc
    30541 agggaaacat ctaagtcggg aaactcgcgc gccgccagtt cggacgcaga ataaatcgac
    30601 gcgccggctt cggaaacgac gattttgtgc aggctgcttt cgggcattcc gcgcaccagt
    30661 tcgcccgcga ttttgtcggt ttcgcggctg gcggtgccgt tgccgatggc gatgagcttc
    30721 acaccgtgtt gtttaatcag gcgcgacaac gttgccaaca tattgttttc ttgatgcaaa
    30781 tagacaatga ccgtatccag cagtttgccc gtgtcgtcca ccacggcgca tttcacgccg
    30841 ttgcggtagc cggggtcgag tcccaaagtg gtcagccgcc cggcgggcgc ggcgagcagc
    30901 aagtctttga gattgcgggc gaacacggta atcgcgtcag tgtcggcagc ttctttcaaa
    30961 cgattgagag cttcgagttc cagcgacaaa aagattttcg cgcgccaagt caggcgcacg
    31021 gtgtcgcgca gccatttgtg gccgtctgaa accttgaagc ggcgggcgat gatttgctcg
    31081 tattcgcttt gctgcgtaat cggcgtgtcg tcgggctggt atttgagcgc gatgttcaac
    31141 acgccttcgt tgcgaccgcg caaaaccgcc agcgcgcggt ggctgggcat ggcgcgcacg
    31201 ggttcgcggc ggtcgaaata atcgctgaat ttttcgcctt cggtttcttt gccttcaacg
    31261 acttgcgtgt ggatttcggc ttcgttccac agcttgtcgc gcagcgtgcc gatgagttcc
    31321 gcgtcttcgg caaactgctc catcagaatc gcgcgcgcgc catccagcgc ggctttggcg
    31381 tcggggatgt tttcgttcag gtagccttgc gcggcggctt ccacgtcctg cggctgttcg
    31441 gcaagcaaca cgtccgccag cggctgcaaa ccgtgttcgc gagcgatttg cgctttggtg
    31501 cggcgtttgg gtttgtaggg caggtacagg tcttccaacg cggttttgtt gtcggcggct
    31561 tcgatttgcg ccctgaggtc gtctgaaagc ttgccttgct cttcaatgct ttttaacaca
    31621 acggccttgc gctcttccaa ctcgcgcagg tattgcagcc gctcggcaag ctggcgcagc
    31681 tgcgtatcgt ccagcccgcc cgtggcttct ttacggtaac gcgcgataaa ggggacggtc
    31741 gcgccgtcgt ccaaaagctc gatagcggca ttgatttgca cggtagtggc ggagagttct
    31801 tgggagagga ttcgggtaat gttcataaat tgcctccgtg atgccgtctg aacagccgac
    31861 ggcgcaatgt gggtaatcgt ttggaaaaga tgaagataat actgatatac tttgcccgat
    31921 acaatctgaa aggaatgttt atgaccgtcc tgattaccgg cggcaccggc tttatcggtt
    31981 cgcacaccgc cgtctcgctc gtccaatccg gttacgatgc cgtgattttg gataatctgt
    32041 gcaactcgtc tgccgccgtc ctcccgcgcc ttcggcaaat taccggcaga aacataccgt
    32101 tttatcaggg cgacatccgc gactgtcaga ttttgaggca gattttttca gaacatgaaa
    32161 tcgaatccgt catccatttt gccggtttga aggcagtggg ggaaagcgtt gccgagccga
    32221 caaaatatta cggcaacaat gtttacggca gcctggtgct ggcggaagaa atggcgcgcg
    32281 cgggcgtgtt gaaaatcgta ttcagctcgt cggcaaccgt ttacggcgat gcggaaaaag
    32341 tcccctatac ggaagatatg cgcccgggcg ataccgctaa tccttacggt gcgtccaaag
    32401 cgatggtgga gcggatgtta accgacatcc aaaaagccga tccgcgttgg agcgtgattt
    32461 tgttgcgcta tttcaacccg atcggcgcgc acgaaagcgg acttatcggc gaacagccca
    32521 acggcgttcc caacaatctt ttgccctata tctgtcaagt ggcttcgggc aggctgccgc
    32581 aactgtcggt attcggcggc gactatccga cccccgacgg tacgggaatg cgcgactaca
    32641 tccatgtgat ggatttggca gaagggcata tcgcggcaat gaaggcgaaa ggcggcgttg
    32701 ccggcgtaca tttgttcaac ttgggttcgg gacgcgccta ttccgttttg gaaatcatcc
    32761 gcgcctttga ggccgcatcc ggtttgcaca ttccttaccg aatccaaccc cgccgcgccg
    32821 gcgacttggc gtgttcctat gccgacccgt cccataccaa acaacaaacc ggctgggaaa
    32881 ccaaacgcgg cttgcagcaa atgatggaag attcgtggcg ttgggtcagc cgcaaccccg
    32941 gcagatatgg ggattaaatg caaaccgcag gcaaaaaaaa catcctcgtt accggcggcg
    33001 cgggttttat cggctcggca gtcgtccgcc atattatcca aaacacccga gattccgtcg
    33061 tcaacctcga caagttaacc tacgcgggca atctcgaatc gctgaccgac attgccgata
    33121 atccccgcta cgcttttgag caagtggata tttgcgaccg cgccgaactc gaccgcgtgt
    33181 tcgcgcaata ccggcccgat gccgtgatgc acttggcggc ggaaagccac gtcgaccgcg
    33241 ccatcggttc ggcaggcgaa tttatccgaa ccaatatcgt cggcacattc gacctgctgg
    33301 aagctgcgcg cgcctattgg cagcaaatgc cgtctgaaaa acgcgaagcc ttccgtttcc
    33361 accatatttc taccgatgaa gtctatggcg atttgcacgg cacagacgat ttgtttacag
    33421 aaaccacgcc atacgcgccg tccagtccct actccgcctc caaagccgcc gccgaccacc
    33481 ttgtccgcgc gtggcagcgg acttacaggc tgccctccat tgtcagcaac tgttccaata
    33541 attacggccc ccgacaattc cccgaaaaac tcattccttt gatgattctg aacgcacttt
    33601 ccggcaaacc actgcctgtg tacggcgacg gggcgcaaat ccgcgactgg ctgtttgtcg
    33661 aagaccacgc gcgcgcgctg tatcaggtcg ttaccgaagg tgttgtcggc gaaacctaca
    33721 atatcggcgg acacaatgaa aaaaccaatc tcgaagtgat caaaaccatc tgcgccctct
    33781 tggaagaact cgcccccgaa aaaccggccg gcgtggcacg gtatgaagat ttaattactt
    33841 tcgtacaaga ccgccccggc catgatgccc gctacgccgt cgatgcggcg aaaatcaggc
    33901 gggatttggg ctggctgcct ttggaaactt ttgaatccgg cctccgcaaa accgtgcaat
    33961 ggtatttgga caacaaaacc cggcggcaaa acgcataaaa cggcaactgc cgttcggaac
    34021 gcttaggtac gggcaaatag ttttcagacg gcatcccgac gcaatgccgt ctgaaaaccc
    34081 atcgcaaagg aagaaagaaa agatgaaagg catcatactg gcaggcggca gcggtacgcg
    34141 cctatatccg attacgcgcg gcgtatccaa acaactgctg cccgtgtacg acaaaccgat
    34201 gatttattac cccttgtcgg ttttgatgct ggcgggaatc cgcgatattt tggtaattac
    34261 cgcacccgaa gacaacgctg ccttccaacg gctactcggc gacggctccg acttcggcat
    34321 ccgcctgcaa tatgccgttc aacccagccc cgacggcttg gcgcaggcat ttatcatcgg
    34381 cgaagaattt atcggcaacg gcaatgtctg cctgattttg ggcgacaata ttttctacgg
    34441 tcaatcattt acgcaaacat tgaaacaggc cgccgccaag acccacggcg caaccgtatt
    34501 cggttatcgg gttaaagacc ccaagcgttt cggcgtggtt gaatttgacg aaaacttcaa
    34561 cgccttatcc atcgaagaga aaccgcaaca gcccaaatcc gattgggcgg taaccggctt
    34621 gtacttccac gacaaccgcg ccgtcgagtt cgccaaacag ctcaaaccgt ccgcacgcgg
    34681 cgaattggaa atttccgacc tcaaccggat gtatctggaa gacggctcgc tgtccgttca
    34741 aatattggga cgcggtttcg cgtggttgga caccggcacg caagaaagcc tgcacgaggc
    34801 ggcttccttc gtccaaaccg tgcaaaatat ccaaaacctg cacatcgcct gcctcgaaga
    34861 aatcgcctgg cgtaacggct ggctgacgaa aaaagatgtg gaaacacgcg caaaacattt
    34921 ggaaaaaacc gcctacgggc aatacctgct gcacctaatc ggcaaataaa taccgtctga
    34981 acgccccgac ggctttcaga cggcatccaa accgtgccgc gccgccacct taaccgacct
    35041 gccgcacggc gcgcataaaa aatcaaaccg caaaaaacag cggtaacgat accgccaaag
    35101 gactttatgg acatcatcga caccgccctc cccgacgtaa aacttttaaa accgcaagtc
    35161 ttcacagacg ggcgcggttt ttttatggaa acattccgcg acggatggtt caaagaaaat
    35221 attgccgatc gaactttcgt gcaagaaaac cactccaact ccagcaaagg cgtattgcgc
    35281 ggcctgcact accaaaccga aaacacccaa ggcaaactcg tacgcgtagt tgtcggcgaa
    35341 gtgttcgacg tggccgtcga tatgcgcgaa ggttcgccga ctttcggcaa atgggcgggc
    35401 gcaaccttgt cggcgcaaaa ccgataccag ctttggatac ccgaaggctt cgcacacggt
    35461 ttttgcgttt tgggcgacgc ggcggaagtc gtgtacaaat gcacggacta ttacaaccct
    35521 gaaaccgaac aggttttaat atggaacgac ccggcaatcg gcataggctg gccgcttcaa
    35581 accgcgccgc tgctttcgcc caaagacctt gccggcaaaa cgtgggcaca agccgaaaag
    35641 ctccgcctta cgctttcccg ataaaaaatg ccgtccgaac gtttcagacg gcattttctc
    35701 cgacaggcta cctacccgcc ttcagtacgc gctgtgcaaa gaaaaacatc ccggtaacga
    35761 agaacgccaa gcccaaccaa gaagcggcgg catcccagtt ttgcagattc aacgcgagcg
    35821 gcgcatccga gccgccgttg gtcacagaga aggcaataat aaacgccata attacaccaa
    35881 tcaggctttc accgacaatc aggcctgccg cgaaaagcgt accgatacgg tcggcgtttt
    35941 tcagacggcc ttcgcggttt tccgccttct tgccaatgat gtgtttcaac accgccgcca
    36001 acaccgcgcc tgccacgatg ggcatattga cggacggcgg cagataaatg cccataccga
    36061 ccgcaaggac gggcagggca agtttaccgc ctgatgattt tttcaacacc aaatcgacga
    36121 cgattaatac cgctccaatc gcgataccgg taaagatgta cgcccattcg aggttgtggg
    36181 cgaaaatgcc cgacgcaatg gtcgtcatca aagtcgcttg aggggctgcc aaagcctgcg
    36241 ccgcatccat gccttcgcgc ggcattgcgc cggtaaagcc gtaggcttcg taaagcagtt
    36301 ccaacacggg cgaaataacc agcgcaccaa cgatacagcc gataatcagg gcgacttgct
    36361 gccgccaagg cgtagctttg agcaggtagc cggttttcaa gtcttgcagg ttgtcgttgg
    36421 aaatcgaagc cacgcagatt actgccgatc cgcaaaaaag cgtcaacgcc agtaagaatt
    36481 tgcggttggc ctcatccgcc aacaaacctc cggattcgcc taccagcagc aaaaccagtg
    36541 aaataacgac gatggacacg atgcccacgc cggaaatcgg gctggaagac gagccgacca
    36601 aacctgccat ataaccgcag gcggcggcga ccaaaaagcc gatgacggaa gccaaaagcg
    36661 tgcaaaccgc caccaaaagc cacgccatgc cgcccgtaat gtgcgaatcg ccgataaagt
    36721 ggtaaaacga cacgcctaga ataaacatca tagacaacac ccagaaaatc atggctttag
    36781 gcgacaaatc ctgttcggcg cgttctgtag cgggcgcacc gccgccaaaa ctcttgaacg
    36841 acatcttcat gccttccacc attggcttga gcagcatcaa aagcgtccaa accgccgcga
    36901 tacctatggt tcccgcaccg ataaaacgca ctttttcctt ccacagcttc atcgcaaacg
    36961 ccgccatttc catatcggaa ggttgcggaa tgtgtgagga gaaatacggc acggcaatac
    37021 cccaagcaat cgaaatgccc agcaggatgg cgatgccgcc cgtcagtccg accaaataac
    37081 ccgcgcccaa caagaccagt gaaaagccca tcggcagttg gaaaattgcc gtaccgcttt
    37141 taaaccaata actcgcgcta tcggcaatca cacgcagacc tccggcgcaa aagctcatca
    37201 atcccgccaa cgcgccgccg gccgccagct ctttgatacc gctgccgccc tgacggtcat
    37261 ccccttcttc atgaccgccc actttcaaaa tttcagcagc cgccacacct tccggataag
    37321 gcaaatcgct tttcaccacc atcgcgtaac gcagcggaat ggtgaaaatc acccccaaaa
    37381 tcccgccggc aatacataaa agcgtcgtct gccaaaacgg gaaaccgctc cagtagcccg
    37441 ccatcagcaa accgggcagg acgaagatga tggttgaaag cgtacccgca gccgaggctt
    37501 gggtctgcac catgttgttt tccaaaatat tgctgccttt gaaaaatttt aaaaccgcca
    37561 tcgaaatcac cgccgccgga atcgacgagg caaaggtcag cccgactttc aaaccgaggt
    37621 aaacgttaga cgcagtaaaa attacagtga tcaatgcgcc gagtatcatg cctcggagcg
    37681 tcagttcgcg gtatttttct accgaaccgg ataaagattt attcattatt cttcctttga
    37741 caacagacgt tcacatattg ttggcatcac gccatgatgt caagttttaa aaagaacagt
    37801 taaaaacaat tatcccaccc tgcccatacc caattgaaaa taaaaactat ttccaaacga
    37861 acaaaaacag ccgtatcagg gaggttcccc gatacggctg cttgtttccg aaccttaaaa
    37921 tcaatcaaac aaatcgcgca gcttgtctaa aaacgatttc ttgcgcggcg tttggttttc
    37981 caagcccgta gaaatccgct caaattcttc caaaagctct ttttgacggt cggtcaaatt
    38041 gacaggcgtt tcgacaacaa tatggcagta caaatcgccg gtcgcgctgc tgcgtaaaga
    38101 tttgacaccc ttacctttca cgcgcatcct cctgccggtt tgggtttctt tggggacggt
    38161 gagcttgact ttgccgtcca aggtcggcac ttctaactcc ccgcccaaag cagccgtggc
    38221 aaaactaatc ggcagttcgc aatgcaaatc caaaccatcg cgttggaaaa tcttatgcgc
    38281 ccgaatgcgg acggttacat acaggtcgcc ggcaggcgcg ccgtgcatac ccggcccgcc
    38341 ttcaccgctc aaacggatac gctgcccgtc atcgataccg gcgggaatat tgacttccac
    38401 cgtcttgacc gccttattcc gccccacgcc acggcatttg acgcaaggtt ctttaatatg
    38461 tttgcccgca ccgcggcagg tcggacaagt ctgctgcata cggaaaatcg cctgctggat
    38521 gtgcaccgta cccgaacctt tgcaagtcgg gcaggtttcc ggggatgccc ccggtttcgc
    38581 gccgctgccg ttgcagacat cacacgcttc ataagtcgga atattgatgc gtttcttcac
    38641 accttttgcg gcttcttcaa gcgtgatttc gataccgacc tgcacgtcct caccctgata
    38701 atcaggctgg gcgcgccccg aaccacctcc aaacatttgg ctgaaaatat ccccaaagtc
    38761 aaaaccctgc gcaccgccaa atccgccaaa ccctccgaag cccccctgtc cgccgccttc
    38821 aaacgccgca tgaccatact ggtcgtacat ggcacgcttt tccttgtcgg acaaagtttc
    38881 atacgccttt tgtacttctt taaacttctc ttccgcctct ttattgtcag gattgcggtc
    38941 gggatggtat ttcatcgcca atttacggta ggctttttta atctcatcat cggtagctgc
    39001 tcttgccaca cccagcgtcg cataaaaatc ttgattactc attttttcat ctaaaaataa
    39061 aatcacggct taaaatgagg gcaattgcgc aaaacacaag acaaacaga
//
LOCUS       DAXFHF010000012        39101 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXFHF010000012 DAXFHF010000000
VERSION     DAXFHF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39101
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.012"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            194..2200
                     /locus_tag="ACFIRM_000525"
     CDS             194..2200
                     /locus_tag="ACFIRM_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC3028220.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2323..3414)
                     /gene="mltB"
                     /locus_tag="ACFIRM_000526"
     CDS             complement(2323..3414)
                     /gene="mltB"
                     /locus_tag="ACFIRM_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC3028221.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3562..4989)
                     /locus_tag="ACFIRM_000527"
     CDS             complement(3562..4989)
                     /locus_tag="ACFIRM_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3028222.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEEQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5174..8878
                     /gene="mfd"
                     /locus_tag="ACFIRM_000528"
     CDS             5174..8878
                     /gene="mfd"
                     /locus_tag="ACFIRM_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC3028223.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDGIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9104..9406
                     /locus_tag="ACFIRM_000529"
     CDS             9104..9406
                     /locus_tag="ACFIRM_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC3028224.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9568..9951
                     /gene="panD"
                     /locus_tag="ACFIRM_000530"
     CDS             9568..9951
                     /gene="panD"
                     /locus_tag="ACFIRM_000530"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC3028225.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9973..10815
                     /gene="kdsA"
                     /locus_tag="ACFIRM_000531"
     CDS             9973..10815
                     /gene="kdsA"
                     /locus_tag="ACFIRM_000531"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC3028226.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10830..11270
                     /locus_tag="ACFIRM_000532"
     CDS             10830..11270
                     /locus_tag="ACFIRM_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC3028227.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11317..12603
                     /gene="eno"
                     /locus_tag="ACFIRM_000533"
     CDS             11317..12603
                     /gene="eno"
                     /locus_tag="ACFIRM_000533"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC3028228.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12771..13049
                     /gene="ftsB"
                     /locus_tag="ACFIRM_000534"
     CDS             12771..13049
                     /gene="ftsB"
                     /locus_tag="ACFIRM_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC3028229.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13090..13380)
                     /gene="yfaE"
                     /locus_tag="ACFIRM_000535"
     CDS             complement(13090..13380)
                     /gene="yfaE"
                     /locus_tag="ACFIRM_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC3028230.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13501..14634)
                     /gene="nrdB"
                     /locus_tag="ACFIRM_000536"
     CDS             complement(13501..14634)
                     /gene="nrdB"
                     /locus_tag="ACFIRM_000536"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC3028231.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14947..17226)
                     /gene="nrdA"
                     /locus_tag="ACFIRM_000537"
     CDS             complement(14947..17226)
                     /gene="nrdA"
                     /locus_tag="ACFIRM_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC3028232.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17757..18068
                     /locus_tag="ACFIRM_000538"
     CDS             17757..18068
                     /locus_tag="ACFIRM_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC3028233.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(18118..18228)
                     /locus_tag="ACFIRM_000539"
                     /pseudo
     CDS             complement(18118..18228)
                     /locus_tag="ACFIRM_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18331..19098)
                     /locus_tag="ACFIRM_000540"
     CDS             complement(18331..19098)
                     /locus_tag="ACFIRM_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC3028234.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTVRPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(19186..20013)
                     /gene="mutM"
                     /locus_tag="ACFIRM_000541"
     CDS             complement(19186..20013)
                     /gene="mutM"
                     /locus_tag="ACFIRM_000541"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC3028235.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(20066..20731)
                     /locus_tag="ACFIRM_000542"
     CDS             complement(20066..20731)
                     /locus_tag="ACFIRM_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC3028236.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            20910..22885
                     /locus_tag="ACFIRM_000543"
                     /pseudo
     CDS             20910..22885
                     /locus_tag="ACFIRM_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            22953..23645
                     /locus_tag="ACFIRM_000544"
     CDS             22953..23645
                     /locus_tag="ACFIRM_000544"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC3028237.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            24119..25489
                     /locus_tag="ACFIRM_000545"
     CDS             24119..25489
                     /locus_tag="ACFIRM_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3028238.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVVVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITAIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            25722..26378
                     /gene="cmk"
                     /locus_tag="ACFIRM_000546"
     CDS             25722..26378
                     /gene="cmk"
                     /locus_tag="ACFIRM_000546"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC3028239.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26531..28216
                     /gene="rpsA"
                     /locus_tag="ACFIRM_000547"
     CDS             26531..28216
                     /gene="rpsA"
                     /locus_tag="ACFIRM_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC3028240.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28227..28541
                     /locus_tag="ACFIRM_000548"
     CDS             28227..28541
                     /locus_tag="ACFIRM_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC3028241.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(28737..29144)
                     /locus_tag="ACFIRM_000549"
     CDS             complement(28737..29144)
                     /locus_tag="ACFIRM_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC3028242.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            29294..30404
                     /locus_tag="ACFIRM_000550"
                     /pseudo
     CDS             29294..30404
                     /locus_tag="ACFIRM_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            30413..31240
                     /gene="fghA"
                     /locus_tag="ACFIRM_000551"
     CDS             30413..31240
                     /gene="fghA"
                     /locus_tag="ACFIRM_000551"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC3028243.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNPENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            31770..32921
                     /gene="zapE"
                     /locus_tag="ACFIRM_000552"
     CDS             31770..32921
                     /gene="zapE"
                     /locus_tag="ACFIRM_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC3028244.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            32980..33405
                     /gene="ndk"
                     /locus_tag="ACFIRM_000553"
     CDS             32980..33405
                     /gene="ndk"
                     /locus_tag="ACFIRM_000553"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC3028245.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            33549..34643
                     /gene="rlmN"
                     /locus_tag="ACFIRM_000554"
     CDS             33549..34643
                     /gene="rlmN"
                     /locus_tag="ACFIRM_000554"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC3028246.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            34646..35407
                     /gene="pilW"
                     /locus_tag="ACFIRM_000555"
     CDS             34646..35407
                     /gene="pilW"
                     /locus_tag="ACFIRM_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC3028247.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            35423..36688
                     /gene="ispG"
                     /locus_tag="ACFIRM_000556"
                     /gene_synonym="gcpE"
     CDS             35423..36688
                     /gene="ispG"
                     /locus_tag="ACFIRM_000556"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC3028248.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(36742..37356)
                     /gene="clpP"
                     /locus_tag="ACFIRM_000557"
     CDS             complement(36742..37356)
                     /gene="clpP"
                     /locus_tag="ACFIRM_000557"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC3028249.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(37452..38762)
                     /gene="tig"
                     /locus_tag="ACFIRM_000558"
     CDS             complement(37452..38762)
                     /gene="tig"
                     /locus_tag="ACFIRM_000558"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC3028250.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
     gene            38987..>39101
                     /locus_tag="ACFIRM_000559"
     CDS             38987..>39101
                     /locus_tag="ACFIRM_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC3028251.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYL"
BASE COUNT         9889 a        10797 c         9310 g         9105 t
ORIGIN      
        1 gtacagaccc gccgaaattg ttataatcga aaaaattaaa aatcaatacg gattattcta
       61 tcagattgat ttatcgatat ttattatatc attaatatag tttgatttca aaccggcgga
      121 aaatgcgccg cccgccgcag atgcgccctc cccgccatcg gggcgtttaa cgccggattg
      181 ccgatgcggt acaatggcag atatgaagaa aattacccct caaaacctgc gtcccctgct
      241 ttcggaaagc ttgggacata ccgattttgt caacgtcctc aacgcattga ttaaattttt
      301 gcgccgtggc ggcaaaaaat gtgcggggga gcgtttcgac ctgattatcg acacattcaa
      361 acaagacagg gaattactgt cccgcttcag ccggtgtttt tacatctggc tcgcgcaaat
      421 acacatttat ccggcactca tcaaactcgg cattttctcg cgccacagct ttgcccggga
      481 aatgggcata cgcatatacg aacgcttcaa cccgtcatat aaagattttg ccaacttggg
      541 cgaagtcttc ctttatcttt tccattccga aaacgacgac aaatggctgc aaacgctcaa
      601 tatccgccaa tggctggttt tatacgaact catccgtagc cacgccgagc cgtccaaatt
      661 gcagacggcg ggcatccgcc ttgccgatgc gcgtttgcgc gccatcgaaa tgctgtctgt
      721 ctggacggca tccgaagcca tcgaacccga tctcatccgt atcgccccgc gcctgctgga
      781 agccgattct tccttcgtcg ccctccaacg cgaaaccgcc aaactggtcg aacactaccg
      841 caacggcacc gcgccttacg acaccgccca cctcgaagtg atgttcgacc aatgtttcag
      901 ccagattgac tatttgcgtc gcaaagggac gggcgccggc tccggttcgt cggtcaaagt
      961 cgcccacctg ctcgaacgac tccggcagac cgtaggccgt ctgaagctgc tcacagacat
     1021 acaaaccggt gccggcaaca gcaaccgcct gaccatcgcc ctgatgaact ccctcatcta
     1081 cgcggcggtc gaacaataca gcacccgcca cctgcgccgc agcagcatcc gtatgctcgc
     1141 ccgcagcatt accgaaaaca aaagccacca cggcgaacac tacatcaccc gcaaccgcaa
     1201 agaatatttc aaaatgttct actcggcagc aggcggcggc ggcatcatcg ccctgatggc
     1261 gctgctcaaa atccgcatcg gcacactcga cctcagtccc ttcctcgctt ccttgtcggc
     1321 tgggttcaac tacggcatcg gctttatgat catccatatg ctgcactgca ccgtcgccac
     1381 caagcagccc gcgatgactg ccgccagctt tgccgaacag gtcgatctca acgaaggcgg
     1441 caaagcggtg gacaacaaac tctccaagct cctcatcgac gtatgccgct cccaaagcgt
     1501 cgccgtcttc ggcaacgttt ccatcgccat ccttttggcg tgcgccatat cgttcggcta
     1561 tgcccatctg taccggctgc ccatactcga tgcccacacc gccgcctacc agttcaaatc
     1621 catagacatc atcaatcatc ctaccctatg gtatgccgct attgcaggct tgtggctgtt
     1681 ctgctccggc atcatcgcag gttttttcga caaccgcgcc gactacctca acctgcgcca
     1741 acgcctgccc ttcaatccct tgctgcgtaa aatcatgcgc cccgggcccc gccgcgtcct
     1801 cgccgcctac atccacaaac actacggctc gctggtcggc aacttcatct tcggaatgct
     1861 cttgggtatg accggctatt tcggacacct cctcgggctg ccgctggaca tccgccacgt
     1921 cgccttttcc tccgccaacc tcggctatgc cgccgtcagc ggcaacgtcg gctttggtac
     1981 attcgtactc ggcattttca gcgtcctcgc catcggcttg gtcaacctct gcgtcagctt
     2041 cagcctcgcc ctcttcgtcg ccctgcgctc gcgcggcaca aaaatcggca gcatccgcaa
     2101 cctgatcaaa agtttttgga atcagattaa aagcaatcct tgcatacttt tcctcccgcc
     2161 cgccaaagga cagggacatc ctccttcgga caagccttga ccggcaatgc cgtctgaaac
     2221 gggattcgcc ccgaataccg ccctgatgcg ggaaatcccc ataaaaggat gcaaaaatgc
     2281 cgtctgaacc gaaacgtggt tcagacagca ttttaaaaaa cattacaatc ccgggccgcc
     2341 gagcgaattg gcaatgtccc tgaccgccgt tacatacatc cggctgtggt tgtactgcca
     2401 taccgtataa aaattgttca agcccaaata atattcaaac acgccgggtg cggtttccag
     2461 tttgaacaaa accgcctttt catcatctgc gagcgtttcc ccggggatga tgccgtacgc
     2521 cttcaaatcc gccaccgtcc gcgtcagggc ggttttttcg ccaatgattg cctgaacatc
     2581 cgcacccggc gccaacgtcg ccgacaccaa cattttaccg cccgtgcgcc aaccgtgctg
     2641 cttcatataa ttggcaaccg atgccgcgac atcaccgacg ttgccccata tatcccgatg
     2701 tccgtccccg tcataatcca ccgcccattt ccggtagctc gaaggcataa attgcggcat
     2761 acccattgcg cccgcatagc tgcccttaaa ggcgaaaaca tcgccgcctt cttcttttgc
     2821 cagctttaaa agctcgacca attctttttg gaaaaacccg gcgcggcggg gataatcaaa
     2881 gcctaaggtc gccaatgcgt ccgccacacg gaagctgccc gtatttttgc cgtaattcgt
     2941 ttcaatcccg ataatcgcca cgataagctc ggcaggcacg ccgtattttt gcgccacatc
     3001 atcgataacc gcgcggtttt ccgcataaaa cctgcgcgcg ccgtgaaatt tcgccctgcc
     3061 cgaatttccc gtgcggaaca cataccacgg acgcgatgtg gagggtcggt gcataatctt
     3121 gacgatgtcc gccttgtaag ccgctttgtc aaaaaaatcc tgccattccg cctgggaaaa
     3181 atcccctttc ccgacttcat cgtccacaaa acggcggaca tttgcattgg cggcaaaccc
     3241 gctgtcggat accggtacgg ctgccgcgtc aaacgcgggg cggctttctt ttttcatttc
     3301 atccgcgcgg ggggcttggg cttcatttgc ccggggtgtg cgggcctcca tcgccgtaca
     3361 ggcagacaaa gccgccaaac aaattgccag cggcagtatt tttctctttt ccataaacac
     3421 attccgaaca aatagggtaa gtgggaaagc ggcacaaggg tgaggcgcga aatgcggtat
     3481 ccgccgcaat cggcggtttt gcagaatgcc gcgcgttgcc ttttgcgccg cccgaaatct
     3541 gcctgccgcc gtatttgccc gctatccgca atatcggcag tccaatatat ctttgcggat
     3601 gtcgttcagc aggaaggctg tggtgtcttc gtgttcctcc tgtacgaaga caaagagtcg
     3661 ggcgatgatt ttgccgatga agactttttg cagggcgcag gcgtcggtca gggtgtgttc
     3721 ctgcaggaag ctgcggatgt cttcgggcaa agcgtagtcg cgggcaacgg cggcaaagcg
     3781 gacatcggtt tgcaggcggt cgagcagggt ttggtttttg tccaacttaa tgcctgcttc
     3841 tttttcttcg gcggtggcgc ggacgaactg gtcgtaaatt tcggcataaa gcatatcgtt
     3901 cgggccttca aaaatcgtga aggggcggat atcgatggcg atattgccgg ctggatgtcc
     3961 gcgttcaaaa cccttcgcgc ccaagagttt ttgcaacatt tgcgcggcgg cgtaagtgta
     4021 ttccgtggcg agggttttga cgatgttcgc ctccatcaat tgatgggcga cgggcgcgac
     4081 gggcgaaacg gaatggcaga cgtagcggta aaggatttcg gaaacctgat ggcggcgttg
     4141 gatttcgcgg cgttcgtaat cgacgaatct gatatcgttg cggacatatc ggttcaggtt
     4201 gtcaaggatg tattccataa tgccgtgcgt catgccgatc agttgcaggc ggctgcggat
     4261 aaagatgttt tggaacgcgc gcaaaccggc agcgtcgccc cgggagagtt tcatcacggc
     4321 ggttgcaggc atttcggcat cgatgcggtt gacggcgtaa cggacggcgc gcaggccttc
     4381 ggatgcgagg gtttcgcagc ggatgtatgt tttggggacg agcagcaggt cgatgacttt
     4441 ggcgagtttg ccgtttttgc gctctttggc ggcaacgagg aggaagtcgc tttgcgaatt
     4501 gccctgccag tatttcgcgg cgttgacgta aatggtttgt tcgtcggtat attcgtagca
     4561 ggactgcatt tcgcgtgcaa tcgccgcgcc ggaggtttcg ggttcggtaa cgcccaaacc
     4621 gccgccttcg cctttgaaaa tcatgtccaa accttgtgcg acttgctctt cgccgccgaa
     4681 ctcttgcaga ggctgcaaca ccagcgcgcc ttcgatgccg gtacgcagcg taacgggcac
     4741 gccgtaatgc cccgcaatcc ttaggacttc ttggatttca aactgactgc ccttgcgccc
     4801 gccgtgtttt ttgtcgagga agggcaacag caaacccgcc tgcttcaagg caagccattt
     4861 gccttcgggc aggtatcgca tcaggtcgat gccgtctgaa aaaatgcggc ggaatgcgga
     4921 ttcgatgtgc tttaaaaaag cagccgtgtc catggttgac ggctgcgcgc tcggttcggt
     4981 gtgtatcatc ggcttcctct gtcggttccc attaatcggc ggccggtcaa accgcctgcc
     5041 acagtttaga gttaattttc taaactttac cacaaagtgc accgggcaac aatccgccgg
     5101 cctttcagac ggcatcgcgc accctcacgt gctaaaatga ccgtttgcat cactgtccgc
     5161 cgattgccgc actatgacct gccccatccc caaaccccgt gaaaaatccc gttggttcaa
     5221 tctttcgcaa ggctcgctgc ccttggcttt ggcgcgttat ttgccgcaca agcggctcaa
     5281 agccgtgctg acccaagatg cggaacaggc gttgcgcctt cagacggcat ggcggttttt
     5341 ccgtccgcac gacacggcgg tgttcctgcc ggactgggaa acgctgcctt acgagcgttt
     5401 ttctccgcat caggatttgg tgtcggagcg gctgtcggcg ttgtggcaga ttaaaagcgg
     5461 cgcggcggac gtgttgttcg taccggttgc cacggcgatg cagaagctgc cgcccgtgcc
     5521 gtttttggcg gggcgcacgt tttggctgaa aacggggcag actttggata taggccgcct
     5581 gaaaaccgat ttggtggatg cgggctacaa tcatgtttcc cacgttgtcg cggcgggcga
     5641 gtttgccgtg cgcggcggca ttgtcgattt gttcccgatg ggcagcgaaa cgccgtaccg
     5701 catcgatttg tttgacgatg aaatcgacgg cattaaaacc ttcgataccg acacgcagcg
     5761 caccatttcc cccgtttccg aaatccgcct gctgccggcg cacgagttcc ccaccgacag
     5821 cgaggcgcaa aaaatcttcc gcagccgctt ccgcgaggaa gtcgacggta atccgaacga
     5881 tgccgccgtg tacaaagccg tcagcaacgg ccacttcggc gcgggcgtgg aatattatct
     5941 gccgctgttt tttgaaaacg agctggaaac gctgtttgac tatatcggcg aagatgcgct
     6001 gtttgtctct ttgggcgatg ttcatgccga ggcaaaccgc ttttggaacg acgttaaatc
     6061 acgttacgcc atggcgcagg gcgacgaaac ctatccgcct ttgcttccac agcatttgta
     6121 tctctctgcc gatgtgttcg caggccgtct gaaaaactac ggacaagttc tgcctgatgt
     6181 ttccggcaag gcacactccc tgcccgacct tgccgtcaac cgccaatccg acgatccttt
     6241 gcaggcattg aaggattttc agacggcctt tgacgggcgg attttgctgt gcgccgaaag
     6301 tttgggacgg cgcgaaacca tgctcggttt cttgcagcaa aacggtttga aagccaaacc
     6361 tgtgtccgac tggcagggct tcttgtcggc gcacgagccg ctgatgatta cggtcgcgcc
     6421 gttggcttac ggattcaaac tgggcggact gcaatcttca agccaacagc aaactgttcc
     6481 cgcctccgag ggagaaggca aagcggttac cgaccaaacc gaattttccg catccgcaac
     6541 aaaccctctc cccagccctc tcccgcagga gagggaacaa agtgccgccg ccgtttcaga
     6601 cggcctgaaa gcagccgccg tttcaaccga aagcagcctg tatctcgtcg caagtgatct
     6661 gcacgggcaa acccgacagc aatctgcccc ctcccccgtg ggggagggtt ggggagaggg
     6721 caaagcggtt gccgctcaaa gtgccatcgc cgtcatcacc gaatccgatc tttaccaata
     6781 cgtcgcccgt tcccgcgtcc acaaccgccg taagaaacac gccgctgttt cagacggcct
     6841 gttgcgcgac cttgccgaaa tcaatatcgg cgatcccgtc gtgcacgaag aacacggcat
     6901 cgggcggtat acgggcttgg taacgatgga tttaggcggc gaaaccaacg aaatgatgtt
     6961 gctcgaatac gcgggcgaag cgcagcttta tgtgcctgtt tcgcaactgc atttaatcag
     7021 ccgctactcc ggtcaggcgc atgaaagcgt cgccctgcac aagctcggca gcggcgcgtg
     7081 gaacaaggcg aagcgcaaag ccgccgaaaa agcgcgcgat accgccgccg agttgctcaa
     7141 cctctacgcc caacgcgccg cccaatcggg acacaagttt gaaatcaacg agtcggacta
     7201 tcaggcgttt gccgacggct tcggctacga ggaaaccgaa gaccaggccg ccgccatcgc
     7261 cgccgtgatt aaagatttga cgcaggcgaa gccgatggac cgacttgtgt gcggcgatgt
     7321 cggtttcggc aaaaccgaag tcgccctgcg cgccgcgttt gtggcggtga tgggcggcaa
     7381 acaggtcgcc gtactcgccc cgaccacgct tctggtcgag cagcacgcgc aaaactttgc
     7441 cgaccgtttc gccgatttcc ccgtaaaagt cgccagcctt tcacgtttca acaacagcaa
     7501 agccaccaaa gccacgctcg aaggcatggc ggacggtact gtcgatatcg ttatcggtac
     7561 gcacaaattg gtgcaggacg acataaaatt caaaaactta ggtttattga ttatcgatga
     7621 agaacaccgc ttcggcgtgc gacagaaaga gcagctcaaa cgcctgcgcg ccaatgtcga
     7681 catcctaacc atgaccgcca cgccgattcc gcgcaccctc agcatggcgc tcgaaggtct
     7741 gcgcgacttc tcgctgatta ccactgcacc gagccgccgc ctcgccgtga aaacctttgt
     7801 caaaccgttc agcgaaggca gcgtgcgcga agccgtgttg cgcgaactca aacgcggagg
     7861 acaggtattt ttcctgcaca atgaagtaga tacgattgag aatatgcgcg agcggctgga
     7921 aaccctgctg cccgaagccc gtatcggcgt ggcgcacgga caactgcgtg agcgcgagct
     7981 ggaacaagtc atgcgcgact ttttgcagca aagattcaat gtgttgctct gttccaccat
     8041 catcgaaacc ggcatcgaca tccccaacgc caacaccatc atcatcaacc gcgccgacaa
     8101 attcgggctg gcccaactgc accagcttcg cggacgcgtc ggccgcagcc accaccaagc
     8161 ctacgcctac ctgctcacgc ccgaatacat taccaaagac gcagaaaaac gcctcgacgc
     8221 catcgcggcg gcagacgaac tcggcgcagg tttcaccctc gccatgcagg atttggaaat
     8281 ccgaggcgca ggcgaaatcc tcggcgaagg acaatccggc gaaatgatgc aggtcggctt
     8341 cacgctctac accgaaatgc tcaaacaggc cgtgcgcgac ctcaaaaaag gccgccaacc
     8401 cgacctcgac gcaccgttgg gcattaccac cgaaatcaaa ctgcacagcc cggcattact
     8461 gcctgaagac tactgccccg acatccacga gcgcctcgtc ctctacaaac gcctcgccgt
     8521 atgcgaaacc gtgcagaaaa tcaacgccat acacgaagaa ctcgtcgacc gcttcggcct
     8581 gaccgaacaa cccgtcaaaa ccctcattga aagccaccac ctgcggcttg cggcaaaaga
     8641 actgggtatt gatgccatcg atgccacaag tgaagccgta accgttacct tcggcaaaca
     8701 ccactgtatc gacccgacgg ggattatcct gctgattcag acggataaaa aataccggct
     8761 ggcaggggca gacaaattga ggtttgcagc ggaaatggaa aacatcgagg tcaggatcaa
     8821 taccgtcaaa accgtactaa aaaccctgca aggcaaacgc ctgcccaaag gaaattgatg
     8881 taacgatgcc gtctgaagaa caccgttcag acggcataca atcccccaat cctcaccaaa
     8941 aggaacaatc atgtcgattt ttcaccccta tttccaacaa ctgtccaccg aaggttttga
     9001 cggagaagaa gatgtcctct gggaagacga gctgaccgtc aaaggcaatc ttgtcgaggt
     9061 attcctgtag gcagaaaatg aaaacagcct gccaaccgaa acattggatg catttgccgc
     9121 cttcctgtcc gaattggaaa aagccgacga tgctgcacgc gccgcactga cggaatattt
     9181 gaaacgggac agccgctata tcgactttca caccgaagat acccaaacaa gccgtgatgc
     9241 ggcaaaattt gtccgcacta tgcagttgat ctatatcagc ttatgggcaa aaaatccggc
     9301 ttttgccgtt atggattata tgcccgccaa tatggaaagc gacgaaatcc ttgccgtcaa
     9361 actgcatttg gacggcagca ttttctcaat cgattgggag agttgagccg gattgccaat
     9421 cggcaaaaat gaaatgccgt ctgaaaacag cttccctgcc cgaagccttg ccattatgaa
     9481 tttgaaggaa actccactat aatacggcat tcagattttc agacggcatc acgcccgtca
     9541 gaccgcacac aaaccaaaag gaaatacatg ttccgtacta tacttggcgg aaaaatccac
     9601 cgcgccaccg taaccgaagc cgatttaaac tacgtcggca gcattaccgt cgatcaagac
     9661 ctgttagacg cggcaggcat ctgccccaac gaaaaagtcg ccatcgtcaa caacaacaac
     9721 ggcgaacgtt ttgaaaccta taccattgca gggaaacgcg gcagcggcgt gatttgcctg
     9781 aacggtgctg cagccaggct ggtacagaaa ggcgacatcg tcatcattat gtcttatatc
     9841 caactttccg aaccggaaat cgccgcacac gaacccaaag tcgtcttagt ggacggaaac
     9901 aataaaatcc gcgacatcat ctcctacgag ccgccgcaca ccgtactgta atcccgcaaa
     9961 cggacatcga ttatggatat taaaatcaac gacatcaccc tcggcaacaa ttcgcctttc
    10021 gtcctattcg gcggcatcaa cgttttagaa gatttggatt ccaccctcca aacctgtgcg
    10081 cattacgtcg aagttacccg caaactgggc atcccctata tctttaaagc ctctttcgac
    10141 aaggcaaacc gctcgtctat ccattcctat cgcggcgtag gcttggaaga aggcttaaag
    10201 atttttgaaa aagtcaaagc agagttcggc atccccgtca ttaccgacgt acacgaaccc
    10261 catcaatgcc aacccgtcgc cgaagtgtgc gatgtcatcc agcttcccgc ctttcttgcg
    10321 cggcagaccg atttggtggc cgcaatggcg gaaacgggca atgttatcaa catcaaaaaa
    10381 ccccagttcc tcagcccttc gcaaatgaaa aacatcgtgg aaaaattccg cgaagccggc
    10441 aacgggaagc tgattttatg cgaacgcggc agcagcttcg gctacgacaa cctcgttgtc
    10501 gatatgctcg gtttcggcgt gatgaaacaa acctgcggca acctgccggt tattttcgac
    10561 gttacccatt ccctgcaaac ccgcgatgcc ggttctgccg catccggcgg tcgtcgcgca
    10621 caggctttgg atttggcact tgcaggcatg gcaacccgcc ttgccggcct gttcctcgaa
    10681 tcgcaccccg atccgaaact ggcaaaatgc gacggcccca gcgcgctgcc gctacacctt
    10741 ttagaaaatt ttttaatccg catcaaagca ttggacgatt taatcaaatc acaaccgatt
    10801 ttaacaatcg agtaacacgg tttcgcctta tgatgcagac tttccgaaaa atcagcctgt
    10861 atgccgcaac cttgtggctc ggtatgcaga ttatggcagg ttatatcgcc gcaccggtgc
    10921 tgttcaaaat gctgcccaaa atgcaggcgg gcgaaattgc cggcgtattg ttcgacatcc
    10981 tctcttggag cgggcttgcc gtttggggca cggtactggc tgccgccttt gccgccctaa
    11041 cccggcggca aaccgccctg ctgctttttt tattgtccgc ccttgccgcc aaccaatttt
    11101 tggttacacc cgttatcgag gcactgaaat acgggcatga aaattggctg ttgtcggttg
    11161 caggcggatc cttcggaatg tggcacggta tttccagcat gactttcatg gcaaccgccc
    11221 tactttcagc agttttaagt tggcggcttt ccggcaaaga ggccgtctga agccctccca
    11281 ttttttacct cccttcactt cacttggaga acattcatga gcgcaatcgt tgatattttc
    11341 gcccgcgaaa ttttggactc acgcggcaac cccacagtcg agtgtgatgt attgctcgaa
    11401 tccggcgtaa tgggacgtgc ggccgtaccg agcggcgcat ccaccggtca gaaagaagct
    11461 ttggaacttc gcgacggcga caaatcccgc tattccggca aaggcgtatt gaaggccgtc
    11521 gaacacgtca acaaccaaat cgcccaagcc ctcatcggta tcgatgccaa cgagcaatct
    11581 tatatcgacc aaatcatgat cgaattggac ggtactgaaa acaaaggcaa tttgggtgcg
    11641 aatgcgactt tggcggtctc tatggcggtt gcacgcgccg ctgccgaaga ctcaggcctg
    11701 ccgctttacc gctacttggg gggcgcaggt ccgatgtccc tgcccgtacc gatgatgaac
    11761 gtcatcaacg gcggcgaaca cgccaacaac agcctgaaca tccaagagtt tatgattatg
    11821 cccgtcggcg caaaatcttt ccgcgaagcg ttgcgctgcg gtgcggaaat tttccacgcc
    11881 ttgaaaaaac tgtgcgacag taaaggcttc ccgaccacag tcggcgacga aggcggtttc
    11941 gcccccaacc tgaacagcca caaagaagcc ctgcaactga tggtcgaagc ggccgaagcc
    12001 gccggctaca aggcgggcga agacgtatta ttcgcattgg actgcgcgtc cagcgagttc
    12061 tacaaagacg gcaaatacca cttggaagcc gaaggccgct cctacaccaa cgcggaattt
    12121 gccgaatact tggaaggctt ggttaacgaa ttcccgatta tttccattga agacgggatg
    12181 gacgaaaacg actgggaagg ctggaaactg ctgaccgaaa aattgggcaa aaaagttcaa
    12241 ttggtcggcg acgacttgtt cgtaaccaat ccgaaaattc ttgccgaagg catcgaaaaa
    12301 ggcgtagcaa acgcattgct ggtcaaagtc aaccaaatcg gtactttaag cgaaaccctg
    12361 aaagccgtcg atctggcaaa atgcaaccgc tacgccagcg tgatgagcca ccgctccggc
    12421 gaaaccgaag acagtaccat tgccgacttg gcagtcgcca ccaactgtat gcagattaaa
    12481 accggttctt tgagccgttc cgaccgcatg gcgaaataca accaactgct gcgtatcgag
    12541 gaagaattgg cggaagccgc ctactacccc ggcaaagccg cattctacca actgggcaaa
    12601 taaataaaaa agtggatgta tgtttcaaca cacagggcaa cacatgaagc gtcgccctgt
    12661 gtgttgacct gatttggaag aggttacacc tctcccaaat aaagtctaat tttaccgcca
    12721 cgaaggacgg acatccgagt ggtggggttt caaccagtaa ggaaacgttg atgaagtggg
    12781 taactgtcgt tttatccttc gcacttgtct gttgccaata cagcctgtgg ttcggcaaag
    12841 gcagcatcgg acgcaacagc agtctgagag aacagattgc cgttcaagaa gaaaaaaacc
    12901 agacactcgc cctacgcaat cattcccttg ccgccgaagt ctatgatttg gaaaacggtc
    12961 aagaagccat ttcggaaatc gcccgggtag aactgggtta tatccaagac ggcgaaacct
    13021 tttaccgact catcaggcat aaccggtaat accgtcaaaa agccgtccga accaatgttc
    13081 ggacggcttt tatttcaaca aactgtcaga cagcccctca tcctcccccg ccaaaccgca
    13141 atccagcctg acatccccct cgacgcaaca gcagcacggc agtatctcgt cccgccccaa
    13201 aaaagccaaa ggcggctccc gataagtaac gcttccctcc aaaatcttta cccggcacga
    13261 tccgcaatat ccgcttcggc actgatattc caccatatgc cccgtccgtt ccaagccttc
    13321 caacagggtc tcaccctcca agagctcaaa aaggccctta ttcgtaccaa tgcgcgccat
    13381 ttccgaccaa tcaaaaataa aaggaacaat aaccgatcct acctgctgtt tttcccatca
    13441 tacaacacac aaatgccgtc tgaaacatcc ggcttcagac ggcatttttt caaaaaacat
    13501 ttacaactca aaatcgccca agtcatccgt attcacttca gaatcgattt gaccaatcaa
    13561 ataagaggat atttccactt cctgcggcgc gacctgtacg ttgtcggacg acagccacgc
    13621 attaatccac ggaatcgggt tttgatttgc gccttcaaat ccggccggca gccccaccgc
    13681 ctgcatacgc agattggtaa tatattcgac gtattgggat aagatttctt tgttcaaacc
    13741 gatcatcgaa ccgtctttaa acaaatatgc cgcccattct ttttcctgtt ccgccgcttt
    13801 tttgaagagt tggaaacatt cgtcctgcaa ctcggcggca atttccgcca tttccggatc
    13861 atcaacaccc gaacgcatca gattaagcat atgctgcgtg ccggtcaggt gcagggcttc
    13921 atcgcgggca atcagtttga tgattttggc gttgccttcc atcaactcac gctcagcaaa
    13981 agcaaacgag caggcaaatg aaacgtagaa acggatggct tccaacacat tgacgcacat
    14041 caggcagaga tagagttttt tcttcaaccc gcgcaaagac acagtaacgg gttttccgcc
    14101 gacattgtgc accccttcgc ccaacaggtt gtaatactgg gtgtattcga ttaaatcatc
    14161 ataatagcag gcaatgtctt cggcgcgggc ggtaatgtat tcgttttcga caatatcatc
    14221 aaacacgacc gacggatcat tcacaatatt gcggatgatg tgggtatagc tgcgcgagtg
    14281 gatggtttcg ctgaagctcc acgtttcaac ccaagtttcc agctcgggaa tcgaaaccaa
    14341 aggcagcaag gcaacattcg gactgcgccc ttggatggaa tcgagcagtg tttgatattt
    14401 cagattgctg atgaaaatat gtttttcgtg ttcgggcagg ttggcgtagt cgatacggtc
    14461 gcgcgacacg tcgatttctt ccggccgcca aaaaaacgac aattgttttt caatcagttt
    14521 ttcaaatacc tcgtatttct gctggtcata acgggcgaca tttaccggct gaccgaaaaa
    14581 catcggctct ttcagcgcgt cgtttttggt tttgggaaag gtgctgtatg acatgactag
    14641 gtgttcgcag gacataacgt ctctcttttt aaaaattagt cgacttttct attaattttt
    14701 aattcaccat ataaaacatg ttgaattcta tcaataaagt gccatcctaa aatagcaaat
    14761 actaaaagaa cactaataaa tatataaaat ttatattcaa ctaagaatga ccgaatgaca
    14821 tccatacttt tccttttctg gaaaataaca acttagcctc atatagtagt aggcactaat
    14881 ttaagaaaat ccttgaagcc tcctactatc tagaagcctt ggatatattt caaaaattaa
    14941 cctgttttaa atcttacaag ccccaccagc gcagccgtca tcttgaatat cggtctgcgt
    15001 gtcgtccgcg ccgtcgcggg tgttgtggta gtacagggtt ttgacgccgt atttgtaggc
    15061 ggtcagcagg tctttgagca tttgtttcat agaaactttg ttaccttcga atttgcccgg
    15121 gtcgtaagca gtgttggcgg aaatcgcctg atcgacgaat ttttgcatta cgccgacaag
    15181 tttcaggtag ccttcgttgc ccggaagctg ccacagggtt tcgtaggcat ttttcaaggt
    15241 ttcaaactcc ggcacgactt gtttcaaaat gccgtctttc gatgctttga ccgttaccaa
    15301 tccgcgcggc ggctcgatgc cgttggttgc gttggcgatt tgcgagctgg tttcagacgg
    15361 catgagcgcg gtcagagtag agttgcgcag gccgtatttg acgatgtcgg cacgcaggct
    15421 ttcccagtcg taatgcaaag gctcgccgca gacggcatcc aaatcttttt tgtaggtgtc
    15481 gatgggcagt ttgccttgcg aataaacggt ttggttaaag agcgtgcacg ctccgtattc
    15541 tttggcaagg tttgccgatg ctttgagcag gtaatactgt atggcttcaa aggtacggtg
    15601 ggtcagaccg agcgcggaac cgtcgctgta gcggacaccg tttttcgcca gatagtaggc
    15661 gtagttgatg acaccgatgc cgagcgaacg ccggtccata gtagaggtac gcgcggcttc
    15721 taccggatat ccctgataat ctaaaagtgc atcgagtgcg cgcacggtca aatcggcaag
    15781 cccttccaat tcgtccaagc tgtttaatgc acccaagttg aaggcggaca gtgtacacag
    15841 ggctatttcg ccgtcagggt cgttgatgtt gtccagcggt ttggtcggca gggcgatttc
    15901 catacacaag ttggactgat gaacaggcgc aacgcgcgga tcgaacgggc tgtgcgtatt
    15961 gcagtgatcg acgttttgaa tgtagatgcg cccggtcccg gcacgctcct gcatcagcgt
    16021 ggaaaacagg tcggcagccg gaatgatacg cttgcgtatg ttcgggtctt gctcgtattt
    16081 cgtatagagc cgctcaaatt cgtcttggtc ggcaaaaaac gcttcgtaca accccgaaac
    16141 ctcgttgggc gaaaacagcg taatgttgcc gcccttaatc aggcgggtgt acagcaggcg
    16201 gttgatttgc acgccataat caagctggcg gatacggttg tcttccacgc cccggttgtt
    16261 tttcaacacc agcaggcttt cggcttcaat atgccacaag gggtagaaca aggttgccgc
    16321 gccgccgcgc acgccacctt gcgaacaaga tttgaccgcc gcctgaaaca tcttaaagaa
    16381 gggaatgcag ccggtatgcc gggcttcacc gccccggatt tcgctgtcca gcccgcggat
    16441 acgtccggca ttgatgccga tgcccgcgcg ttgggaaacg tatttcacaa tcgcgctggt
    16501 agtggcattg atggaatcca aactatcgtc gcattcaatc agcacacagc ttgagaactg
    16561 gcgcgtaggc gtacgcacgc cgctcataat cggcgtgggc aacgatactt tgaatgtgga
    16621 aacggcatcg taaaaccgtt tgacgtaacc caagcgcacc tctttcgggt atttgctgaa
    16681 aaggcacatc gccaccaaaa catataaaaa ctgcggcgtt tcgtaaatct ggcgggtaac
    16741 gcggttctgt accagatatt tgccttcgag ctgtttgacg gcggcatagg aaaaggacat
    16801 atcgcgttcg tggtcgatat aggcgttcag ttcgtcaaat tcttcgcggc tgtaatcctc
    16861 aaggatatgc ctgtcgtatt ttccggcatc ggtgagcttt ttaacatggt tgtaaaggtg
    16921 cggcggctcg tattcgccgt aggctatttt acgaagatgg aaaatcgcca aacgtgcggc
    16981 aaggtattgg tagtccgggg tatcttccga aattaaatct gcagcggctt tgatgatggt
    17041 ttcgtggatg tcgtcggtgc ggatgccgtt gtagaactgg atgtgcgatt tcaactcgac
    17101 ctgcgacacg gaaacatttt ccaatccttc cgccgcccaa gtaacgacac ggtgaatctt
    17161 atccaaatca atggcttcca atcttccgtc tcgtttggtt acttttaaat cagtcggtgt
    17221 attcatcgct tcctcttcca ctcttgatat tcaagacacg gtcttttcaa ataaattaag
    17281 gcagacaata tagtggattt ttagcatttt ctccagtctt gacaacgatt gcatttttca
    17341 gtttcaggca atgcgcgata aaacccgcct gtcgtatttt tcctataaca tttgttttat
    17401 ctgataattc tttaccgata aaaaacgggt aaattttttg ccttttgacc ggctccggct
    17461 acaaggcgat gaaataagga ttttccgacg aaaaaggaaa acttccggtt ttccgcccct
    17521 gcgaattgtt aaattttgca aagtatgatt tttgccacgc cgccgccgac aaattccatt
    17581 ttcttaccga ttggaattta ttattgagat taatatgtta tttgaatttg catatcaaac
    17641 ggcaagtttt gtcggctgaa tgagtctgaa actgccgaca gtttgcccgt tccatccttc
    17701 catcctatac tggaaggaat tacaaactga aaggatcgtc atgtccgtca ataaagatgc
    17761 ttgccatact gttgtctgca atactgggac tggtatcaac aactgccgct gccggtacgt
    17821 cagaacccgc ccaccgacat accaaacata tcagcaaggc aaacaagcag atgctgcacc
    17881 ccgaatgcag gaaatatttg gaacgccgtg ccgcgtggta ccgatcgcaa ggcaacgtgc
    17941 aggaattgcg cgaaaacaaa aaggcgcgca aagcattccg caccctgcct tatgcggaac
    18001 agaaaatcca atgccgggcg gcttatgagg ctttcgatga tttcgacggc ggcaggttcc
    18061 gccgttaatc ccatataaaa tatgctgtct gaacacaagt taagatggca ttttccatag
    18121 atatagtgga ttaaatttag accggtacgg cgttgcctcg ccttgccgtg ctatttgtac
    18181 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataca acaaactatc
    18241 cttacggcat ttcttttgag gtactcccgt taccgcagcc ttaaggcatc cgcaaaagcg
    18301 aatcataccg ctacattccg acaaccgctt tcagacggca acatccgcat cgcctgcgac
    18361 aacggcacgg atgctttctt cgattttatc ttttaaagca taacggtctt cgctttccgc
    18421 cgcgtccgcc acgcaaacga aatcgacttt gatagtcagt tttttcatag acacgattcg
    18481 ccacaggcaa gtcggcaaac cgacatcggc atacgacgga cggaccgtcc tttttcccgt
    18541 ttcgtcataa taacgcagtg cgaccgccaa aacctttgcc cccgcatcga tggcggattg
    18601 gaacagcgca gccttgaacg gcaggagccc cagtccggag gaagttcgcg cctcagggaa
    18661 aaaactgacg ttttgaccgc gttgcaaggt ttcgcagacg gcgcggttaa tcggttcgat
    18721 gtcgcgccgc gaattgcggt tgatgaacac cgttcccgcg ttctgcccca tcttgcccaa
    18781 taccggccag cttttgattt cctgcttggc gataaagcta ctcggataaa ccgcgctcat
    18841 cgcgaaaata tccagccagg acacgtggtt ggcggcaacc aagacaccgt tcggatgttc
    18901 gggtgcgggt ctgcccacct ccaatccgat atccaaagcc gccaaagcgc ccttgcccaa
    18961 cgctataact gcccgattgc gcgacttggg gcagccgccg tcaataccgc gcaggttttt
    19021 gccggttttg aacagccaga ccgtcaagcg gcacaagcgg cgcagacgtg taaaaaatga
    19081 agctttattt gaagacatgg gcaattcttt cccgataatc ggtgtttgtt gttgaaacgt
    19141 tcggacggca taccgataaa atgccgtcag aaaccgtttt ccgccctatt tctgacagtt
    19201 cgtgcaataa aacgtgccgc gctgccccaa agtttctttc acaaccaaac cgccgcaccg
    19261 gaggcacggc tgattgtgcc gcccgtacac cgtatattcc tgttgaaagt agccgctttt
    19321 gccgtcgctg tccacaaaat ccctcagcgt actgccgccc gtttcaatgg cgcgctgcaa
    19381 caccgctttg acggtttcaa ccaaaaccgc gcactctttc ttttttaggc ggttggcagg
    19441 acggtggggc gaaatgcccg ccctgaacag actctcgttg gcataaatat tgcccacgcc
    19501 gaccacgacg gtattgtcca tcagagcgag tttgaccgcg cgtttctgcg ccttcagccc
    19561 cgcatacaga taatccgtgc aaaatgcctc cgacaaaggc tccggcccca gtttttccaa
    19621 cagcggatga cgttcttcga ttccctcata ccaaagtatc gcgccgaact ttctcggatc
    19681 gcggtaacgc atgaccgtgc cgtctgaaaa cacaatatcg acgtgatcgt gtctgtccgg
    19741 cctgccgata cgtccgtccg acggcgtaaa gatccgcaag ctgcccgaca tccccaagtg
    19801 aatcagcaaa atccccgttt ggaaacggac aatcagatat ttggcacgcc tgccgcagga
    19861 caaaacctgc cggccggaca aaatctcccc caaatcggga ttgatctgcc agcgcagctt
    19921 cagttggcgc aatatcacgg cttccaccgt ttttccttca atatgcggcg cgatgccgcg
    19981 caacgtcgtt tccacttccg gcaattccgg cataacccct cccgacattt cttctgacag
    20041 atgccgtctg aaagacggcc gtcccttaat ccgcaaccct tgccgcaccc gccgcaaggg
    20101 ctttgccgcc ccaatacccg tcccacgcgg gcaggggcgt tacgcccgcc gcccttttga
    20161 ccaaaaccaa accgtacact gcggcacatt gcggccacca acggtcgccc gccttttcca
    20221 taaaccgcca aaagcgtatt tgcccgagcg acgaaacggg aggcagatac accatgaatt
    20281 tcccaaattc aatatcgaaa ccggcatcgg caagctgcct tttcaactcg ggcagcggca
    20341 gacaaaaccg tttttccggc agacggacac cgtcaaacca acggcagaat ccccaaagcg
    20401 aatacggatt gaaacccgtc agcatcaaac gtccggacgg tttcaatatc cggtgcgctt
    20461 ccgacaggat ttgcgaaggt acgccgcatt ccagcgtatg cggcaaaagc agcatatccg
    20521 caaaaacatc cgccaaagcc atattctccg ccgacatcga catatctcgc ggcacacaga
    20581 caatgtcttc agacaggctc agccactgac cgcccacctg aaccgcacac attcccgaaa
    20641 aacggtatga cgccacatac cgattaaaga agtcctgttc caattttgca acataccgcc
    20701 ccatcgccgt atcttcaaac catgcatcca tattgcgtcc gtttcaaaca gattgcctgc
    20761 cgatatttta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc atttccgaat
    20821 ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca tcggacgata
    20881 aaatatgttt attccgccga gtataaatca tgtccaaact caaaaccatc gccctgaccg
    20941 catcaggtct gtccgtttgt ccgggtttcc tatatgccca aaacacctca tcacaccaag
    21001 tcggtttagc gattatgcgg ttaaactctt caatactcga cctgccaccg acaaaacaat
    21061 atttccaatc cggcagcctg tgggacgagc tgcgccaagg cttccggatg ggcgaagtca
    21121 atcccgaact ggtacgccgc cacgaaagca aattcatcgc aagccgcagc tatttcgaca
    21181 gggtcgtcaa ccggagccga ccctatatgt accatatcgc caacgaagtc aaaaaacgca
    21241 atatgcccgc cgaagccgcc ctgcttccct tcatcgaaag cgcgttcgtc accaaagcca
    21301 aatcacacgt cggcgcatcg ggcctgtggc agttcatgcc cgctaccggc aggcattacg
    21361 gcttggaaaa aacaccggtt tacgacggca ggcacgacgt ttacgcagcc accgatgccg
    21421 cactcaacta tctgcaatat ctctatggac tgttcggcga ctggccgctc gcctttgccg
    21481 cctacaactg gggtgaaggc aacgtcggac gcgccgtcaa ccgcgcccgc gaccaagggc
    21541 tcgaaccgac ctacgaaaac ctgcgtatgc ccaacgaaac gcgcaactat gtccccaagc
    21601 tgctcgccgt gcgcaacatt attgccaccc ccaatctttc ggcatgaata tcagcgacat
    21661 agacaacaaa ccctattttc aggcagtcga accgggccgt ccgctcgaca acgaagccat
    21721 cgcccggctt gccggcatca cgcaaagcga gctgctcgcc ctgaatcctg cattcaacgt
    21781 ccccgcgttc atccccaaaa acaaacgcaa actgctgctt cctgtcgcgt ccgtccaaac
    21841 cttccaaagc aactacctca acgccgcacc cgacagcctg ttttcatggg aagtctatac
    21901 gcctgccgcc aaaaccagcc tgtccgacat ctcgacggca accggcatga gcattgccga
    21961 catcaaacgc ctcaacaacc tgaacggcaa ccttgtcaac gcaggacgca gcatccttgt
    22021 cgccaagaac ggcaagaccc ttcatacggc atcggaatcc gtcgtttcca tcgacatcga
    22081 caatacgccc gacacctacc gttccaatat gccggcaggc acggtgaacg tcagcattgc
    22141 ccgaatccaa cccgccgccg cacagacagc ggacattacc gtcgcacctt tgccgcaaga
    22201 aaccgtccgt acggaacccg atccccttgt ccgcattgcc gaacccgccc ttgcgacagc
    22261 cgcagcgcaa cctcaaaccg aaaaacagac tgccatgccg tctgaaaccc aaaccgcaac
    22321 actcgcgcag gtcgtccccc caaacgacat gcaggcggca gacgaactca tgcagcttgt
    22381 tgcccgaaac aacctgcgcc ggcaggctga agaaaccatc tccgccgtca tcggcacgcc
    22441 tgacacagtt gccgaacaca acatttcctc atctccgcaa cataccgttg ccgccgacgg
    22501 caaacgccgg gcacgtttgg aaacgcgcgt agccaaagct gccgacggcg aagccgaaac
    22561 ctccccgctc catgccagca tccaccgcgt tgtagaaggc gacaccctgt tcaacattgc
    22621 caaacgctac aacgtcagcg tagccgacct gattgtcgcc aacaacatca aaggcaacac
    22681 catccaaaaa ggacaggtac tccgcctggc gcaggcagcc cctgcccaaa cccgtattga
    22741 aaaagtatcc tacaccgcgc gcaaaggcga caccttcaaa agtatcgccg cgcgcttcaa
    22801 tatccatatc gacgacatcc gccggctcaa tcccaacctg aacaccatca atccgggaca
    22861 gagggtcaaa ctgattggaa gctgattcgg atacggcaca tgacaggact ttctcagtcc
    22921 tgtctttttc atcccacatt tcccatacca tcatgaaact catcaaatac ctgcaatatc
    22981 aaggcatagg aagccgcaag cagtgccaat ggctgattag gggcggctac gtctccatca
    23041 acggaacctg catggacgac accgatgcag acatcgattc ctcatacgtc gaaacgttgg
    23101 atattgacgg ggaagcagta accgtcgttc ccgaacccta tttctacatc ctgctcaaca
    23161 agcctgaaga ttacgaaact tcgcacaaac cgaaacacta ccgcagcgta ttcagtctgt
    23221 tccccgacaa tatgcggaac atcgatatgc aggcggtcgg caggctggat gcggatacga
    23281 ccggcgtatt gctgattacc aacgacggca aactgaacca caacctgact tcgccgagca
    23341 gaaaaattcc caagctatac gaagtaacgc tcaaacaccc tacaggagaa acgctctgcg
    23401 aaactttgaa aaacggcgtg ccgctccacg acgaaaacga aaccgtttgt gccgccgatg
    23461 ccgttttgga aaatccgacc accctgctgc tgaccattac cgaaggaaaa taccaccaag
    23521 tcaaacgcat ggttgctgcc gccggcaacc gcgtgcaaca ccttcaccgc cggcgattcg
    23581 cacatttgga aacagaaaac ctcaaacccg gggaatggaa atttatcgaa tgtccgaaat
    23641 tctgaaacaa aattccataa tttccctata ttcaaccata tgcttattaa ataagcagtt
    23701 ttacaaaaat taactcaaaa aacagttagt ttacaatata atccgcttgt ctaagtaacg
    23761 cctgttcaag caggattatg agcaagacgt tttccccgta atgtgttttg ccatctatac
    23821 ttctcccctt gtatagatgg ttttctattt caaattccct ctctttcatc atcattgaaa
    23881 tgctatcatt aatatgatta acaaaatttc acttccgtac accgatgtag aaaaataaca
    23941 gattaatctg ttcataaact ccgattcttc ctcatcggtt tatactcatc agaaatattc
    24001 actgaaatat atgcatttta catggaaaat cactaggcag ttgaaaaaca ttcagtcaat
    24061 ttctgttaag aatcacgtgt aaaatttccg ctttatctca atcggacacg gtttcaatat
    24121 gtcttcccct tcaaatacca accgtcaaac ctggtccagc cgtttgactt atatccttac
    24181 cgttgccggc gcgactgtcg gtttcggcgc gacgtggcgt ttcccgtatt tggtcggtga
    24241 aaacggcggc ggcgcgtatg tgtttttatt ctgtatcgcg atgctggtta tcggcatccc
    24301 gatgattttg gtggaaaacg tcatcggacg gcgcaaaggc gtgaacgcgc tggatgcgtt
    24361 cggcggcccg atgaacggca aacctgttgc caaaatttgg aaactggtcg gccggatggg
    24421 gctgctcggc gcgttcggca tcatggctta ttatatggta ctcggtggct gggtaatcag
    24481 ctatatcgtt aatattattg gaggaaattt gaatatttcc agccccgtcg acggtgtggt
    24541 tacaaaaggc ttctttaccg aacacattga aaacagccct tgggaaattg cgttttatac
    24601 gctgcttttt gtcgtcgtga accaatggat tttggtcaaa ggcgttatcg gcggcattga
    24661 aaaaacggcg aaatacctga tgcctctgct gtttttgttc ctaatcgcga tggtcgtccg
    24721 caacgttacc cttccgggcg caatggaagg ggttgctttc tatctgaaac ctgatttcag
    24781 caagattacc gccgaactgt tcgtcttcgt tttggggcag gtattttttg ccctgagcct
    24841 gggtttcggc gtgatgatta ccttatccag ctatctggat aaaaacgaaa atctggttca
    24901 gacggcagtt atcacggcaa ttaccaatac catcatcgct gtacttgcgg gctttatgat
    24961 tttcccgtcg ctcttcagct tcggcgtcgc gcccaattcc ggcccgactt tggtgttcca
    25021 aagtttaccg attgtgttct cacatatgtg ggcaggacct gtgttcgccg tcattttctt
    25081 ctctctgctc ctgattgccg cgttgacaac ttcgctgacg atttatgaag tgttgattac
    25141 ggccattcag gaaaaaacca aaatccgccg taccgccgca atcacgattg tattgtccgt
    25201 catcttcgtt ttcggcaaca tcccgtctat tttgagctat ggcccgtgga aagacgtttc
    25261 cgtgttcggc aaaaatattt tcgatgcctt cgactacatc agcggcaaca tcttgtttat
    25321 gctgaccgcg ctcggttccg cattgtttgt cggttttgtg atgaaggacg aagcgaagga
    25381 agaattgctt tataaaggca accatacgac ggtcaatatt tggtttgcct atgtgaaata
    25441 ccttgtgccg ctggtgattc tgctgatttt cgtcagcaac ctgttctaat ccgcagcaat
    25501 caatgccgtc tgaaggtcat acctctttca gacggcattg cattgatgcc cgccacgccg
    25561 gaatccgaac cggcttgccg gcttctgtcg gacgctttcc gggcaggcgg cgttccgtct
    25621 gcttagacaa ccgtccttta aaacaggtag aatccgcccc aacgggaaac acacccttca
    25681 gacggcaaaa cccatacccc aaaccatcag gaatccccct tatgaacaga caaaaagtca
    25741 tcgccatcga cggtccgggc gcatcgggca aaggcacggt cgccgcccgc gttgccgccg
    25801 cattgggata cgattatctc gataccggcg cgctctaccg cctgaccgcc ctatatgcac
    25861 aaaaacaagg cgtggaatgg cacgatgaag aaaacgtttc cgcacttgcc aaaaaactgc
    25921 ccgccgtatt ttcaggcaac cgcatcctac ttgatggcga agacgtttca gacggcatcc
    25981 ggacagaagc catcggtatg ggcgcatccg cagttgcgca atggcccaaa gtccgcgccg
    26041 cactgctgca acgccaacgc gattttctga ccgaaaaagg actggttgcc gacggacggg
    26101 acaccggatc ggtcgtcttc ccccaagccg aactcaaaat ttttctgaca gccgaatcta
    26161 aaatccgtgc cgaacgccgc gccaaacaaa tcggcattcc ctgcgaaggt ttcacattcg
    26221 agcgcatcct gtccgacatc gagaccagag acgaagcaga ccgaaaccgc aaggttgccc
    26281 ccctgaaaca acagcccgat gccctgcttt tggatacgag ccgcctaact atagaagaaa
    26341 ctgtaaaaaa agtgcttgat tggtatcgta aagtttaaat tttcaggtat aatcgcccgc
    26401 attgcgcttc agacagcatg aatttccatg tgccgtctga aaccttattt acccgcctgc
    26461 cgtgccaagg actgcagaca cccaccaacc caacccgcac cccttggcgg tgtaccgaaa
    26521 agagttatat atgtctatgg aaaattttgc tcagctgttg gaagaaagct ttaccctgca
    26581 agaaatgaac ccgggtgagg tgattaccgc tgaagtagtg gcaatcgacc aaaacttcgt
    26641 taccgtaaac gcaggtctga aatcagaatc cctgatcgat gtagctgaat tcaaaaacgc
    26701 tcaaggcgaa attgaagtta aagtcggcga cttcgttacc gttaccatcg aatccgtcga
    26761 aaacggcttc ggcgaaacca aactgtcccg cgaaaaagcc aaacgcgcag ccgattggat
    26821 cgctttggaa gaagccatgg aaaacggcaa catcctgtcc ggcatcatca acggtaaagt
    26881 caaaggcggc ctgaccgtta tgatcagcag catccgcgca ttcctgccgg gttctttggt
    26941 cgacgtacgt cccgttaaag acacttccca ttttgaaggc aaagagatcg aattcaaagt
    27001 gatcaaactg gacaaaaaac gcaacaacgt cgttgtttcc cgccgcgccg ttttggaagc
    27061 cactttgggt gaagaacgca aagccctgct ggaaaacctg caagaaggct ccgtcatcaa
    27121 aggcatcgtc aaaaatatca ccgactacgg cgcattcgtt gacttgggcg gcatcgacgg
    27181 cctgctgcac atcaccgatt tggcatggcg tcgcgtgaaa cacccgagcg aagtcttgga
    27241 agtcggtcag gaagttgaag ccaaagtatt gaaattcgac caagaaaaac aacgtgtttc
    27301 cttgggtatg aaacaactgg gcgaagatcc ttggagcggt ctgacccgcc gttatccgca
    27361 agccacccgc ctgttcggca aagtatccaa cctgaccgac tacggcgcat tcgtcgaaat
    27421 cgaacaaggc atcgaaggtt tggtacacgt ctccgaaatg gactggacca acaaaaacgt
    27481 acacccgagc aaagtcgtac aactgggtga cgaagtcgaa gtcatgattt tggaaatcga
    27541 cgaaggccgc cgccgtatct ctttgggtat gaaacaatgc caagccaatc cttgggaaga
    27601 atttgccgcc aaccacaaca aaggagacaa aatctccggt gcggttaaat ccattaccga
    27661 tttcggcgta ttcgtcggcc tgcccggcgg catcgacggt ctggttcacc tgtccgacct
    27721 gtcttggacc gaatccggcg aagaagccgt acgcaaatac aaaaaaggag aagaagtcga
    27781 agccgtcgta ttggcaatcg atgtggaaaa agaacgcatc tccttgggta tcaaacaact
    27841 ggaaggcgat cctttcggca acttcatcag cgtgaacgac aaaggttctt tggttaaagg
    27901 ttccgtgaaa tctgttgatg ccaaaggtgc tgttgttgcc ctgtctgacg aagtagaagg
    27961 ctacctgcct gcttccgaat ttgcagccga ccgcgttgaa gacttgacca ccaaactgaa
    28021 agaaggcgat gaagttgaag ccgtcatcgt taccgttgac cgcaaaaacc gcagcatcaa
    28081 actttccgtt aaagccaaag atgccaaaga aagccgcgaa gcactgaact ccgtcaatgc
    28141 cgccgccaat gcgaatgccg gtaccaccag cttgggcgac ctgctgaaag ccaaactctc
    28201 cggcgaacaa gaataaggtt gcagacatga caaagtctga gttaatggtt cgcttggcag
    28261 aagtatttgc cgccaaaaac ggcacgcatc ttctggcaaa agacgtagag tacagcgtaa
    28321 aagtcttggt tgacaccatg acccgatcgc ttgcccgagg tcaacgcatc gaaatccgcg
    28381 gtttcggcag cttcgatttg aaccatcgtc ctgcccgcat cggtcgcaat cccaaaaccg
    28441 gcgagcgcgt ggaagtacct gaaaaacatg taccccactt caagcctggt aaagaattgc
    28501 gcgagcgggt cgacttggct ttaaaagaaa atgccaatta aaccttagca tcaaaacgcc
    28561 gctggtacgc ggcgtttttt ctttggttta acttcatccg ctgcttcaat accttaagcc
    28621 aagcaagcaa cggattagag cgtggatttt ttaccattgc ttacaatccg ctttttaaac
    28681 aacaaattgt tgatttctat tacgaacagg acaaaatcct gcttattgca ctaaaactaa
    28741 gccgtttcag gaatttgcgg caaatttaca gcttttaccg agcctaatgc tttcgctttt
    28801 tggtaaaacg ccaatttgta ttcaagcaaa tctaaataac gttttaattc ggcaatttga
    28861 cacttcacat tttctatttg attttcaaac aaggaaaggc gttcttcaat ggtatcgtcg
    28921 ccaatgacgg tacattccgc aaagcgtttg atgtctttta agctcattcc cgtatttttc
    28981 aagcattgca ataagtacaa ccattgcaaa tcgttatcgg taaaacaacg gttaccgtat
    29041 tcatcacgtc cgatattagg caacaagcct tctttgtcgt aaaagcgtaa agtgtgggcg
    29101 gagatgccta ttttttcggc agctttggca gtggtataag tcattttccc tccctctaaa
    29161 caaaaaacgg taaaaaaaca cttgccttag agtgaactct aaaatgtaaa ctgttgctat
    29221 gttgctcagg caaggctatt ttgttaatga attaagagga aagacaatgg aaatgaaaca
    29281 aaccgattca accatcaaat ctcgtgcggc ggtggcattc gcccctaacc aacccttaca
    29341 aattgtggaa atcgacgtgg aaatgccgcg taaagacgag gtgttaattc gtaacaccca
    29401 cactggcgtg tgccatactg atgcatttac cttatcagga agcgatcctg aaggcgtatt
    29461 ccctgtggtg cttggacacg aaggtgcggg tgtggtcgtt gctgtgggcg agggtgtgtc
    29521 aagcgtaaaa ccgggtaatc acgtcattcc gctttacacc gcagaatgtg gcgaatgtga
    29581 gttttgcctc tcgggtaaaa ccaatttatg cgtctcagtg cgtgatacac aaggtaaagg
    29641 cttaatgcca gacggcacga cgcgtttttc ttatcaaggt cagccgattt atcactatat
    29701 gggctgttcg actttcagtg aatattctgt tgttgccgaa gtttcactgg cgaaaatcaa
    29761 cccgaaagcc aaccacgaac aagtatgttt gctcggctgc ggcgttacca ctggtattgg
    29821 tgcggtacat aacacggcaa aagtacaaga aggcgattct gttgccgtgt ttggcttggg
    29881 agcgattggt ttggctgtgg tgcaaggtgc gcgtcaagcc aaagccggtc gcattatcgc
    29941 cattgatacc aatcctgcaa aattcgagtt ggcaaaacag tgttggtgca actgactgtt
    30001 tgagcccgaa agattacgat aaaccaatca aagatgtgtt gttagacgtc aacaaatggg
    30061 gcattgacca cacctttgaa tgtatcggca atgtaaacgt aatgcgtcag gcattagaaa
    30121 gtgcacaccg aggttgggga caatccatca tcatcggcgt agcggatgca ggacaagaaa
    30181 tttcaacgcg tccgttccaa tcggtaacag gccgtgtttg gaaaggctcg gcatttggtg
    30241 gtgttaaagg ccgctccgag cttccgaaaa tggtggagga ttcaatgaaa ggcgatatcc
    30301 aattagaacc atttgtgacc catgcaatga cccttgatca aatcaatgaa gcctttgagt
    30361 taatgcacga aggtaaatcg atccgcgctg ttattcatta ctaaggtcca cgatgaaact
    30421 gattgaacaa catcaaattt ttggtggttc gcaacaagtt tgggcgcatc atgcccaaac
    30481 gcttcaatgc gaaatgaaat ttgccgtcta tttgccggat aatccggaaa atcaaccgct
    30541 tggtgtgatt tattggcttt ccggcttgac gtgtacggaa caaaatttca ttaccaaatc
    30601 gagcttccgg cgttatgcgg cagaacatca agtggttgtg gtcgcccccg ataccggccc
    30661 tcgcggagag caagtgccga acggtgccgc ttatgattta ggacaggggg caggctttta
    30721 tttgaatgcg accgaacagc cttgggcggc gaattatcaa atgtatgatt acattttgaa
    30781 cgagttgccc cgtctgattg aggagcattt tcctaccaac ggcaaacgtt ccattatggg
    30841 gcattcgatg gacggacacg gcgcattggt attggcacta cggaatcggg aacattatca
    30901 aagtgtttct gccttttcgc ctattttatc gccaagcctc gtaccgtggg gagaaaaagc
    30961 ctttactgct tatttaggga aagaccgtga aaaatggcag caatatgatg ctaactcact
    31021 cattcaacaa ggctataaag tgcaaggtat gcgtatcgat cagggcttgg aagatgagtt
    31081 tttgccgaca caattgcgta ccaaagattt tatcgaaacc tgtcgtgcgg caaatcagcc
    31141 aatcgatgtg cgtttccata aaggatacga tcacagctat tacttcatcg ccagttttat
    31201 tggtgagcat atcgcttatc acgccgcatt tttgaagtaa gtcaaagagc ggtcagtttt
    31261 caaagcagtt tggaatagcc ggcacgaggg cggtaagaag tgccggcata aacgtatgcc
    31321 gcctgaaccg gaaggagccg gccctacgga ttaccaaacc ctgcccttga tttgattagt
    31381 atgattttta agcttaaacc ctgattgatc aaacattaaa atcgcccgta tttcctactt
    31441 cactttcggg cttcgtatta ttcgttgccg aatttgcata agcaacctgt tcctgttgtg
    31501 ttttcgcccc ctccaagctg acggtggcgt aacagcttcg cgattcacca tctgtttcat
    31561 ttgtcctcct tcaatattaa aacgcagcct gaaataaaag gcattttggc tcatatctgc
    31621 accatattaa aacgccgcct ttgcttatac cccctttgtg cgcgtcatta ttcttttcca
    31681 cgggaaacgc caagtttgaa ggaaatcatt tataatacca gcggtaagca ttttctttct
    31741 tagccgcaag aagtataaca aggttaaata tgaataatag agaccaactt tttaaagccc
    31801 cgccgtttga aaaccacagc ccgctgacct ggtatcaggc tgcctcacaa ctgcccaact
    31861 tcatccgcga cgacgcacag gcggccgcca tcgaacacct tgaccggctt tggaccgaat
    31921 tgatgatgtt caaacgcaaa agaaaccgtt ttttaggcag gagtttgcgt tccccgcaag
    31981 ttcccaaagg gctttatttc tatggcggag tcggacgcgg caagagcttt ctgatggacg
    32041 cttttttcgg ctgcctccct taccgccgca aacgccgcgt ccactttcat gcctttatgg
    32101 cggaaatcca ccggcggctg aaagccctga aaagcgaaag caacccgttg aaatccgttg
    32161 ccgccgagat taccaaagaa acccgagtat tgtgttttga cgaatttcat gtcagcgata
    32221 ttgcggatgc aatgatttta ggccgtctgt tggaaaacct gcttaacgag ggcgttgttt
    32281 tggtggcgac ttcaaactac gcgccttccg aactctaccc gcaaggtcaa aaccggagcg
    32341 gttttcttcc cacaatcgcg ctcatcgagt ccagtctgac cgtcttaaac gttgacggag
    32401 gggaagacta ccggctgcgt accctccgcc ccgccgagat tttctttacg cctgccaatg
    32461 aagaaaatga ggcaaaactg gcaaaactgt tcaaagaaat gacaggcatt accgatttga
    32521 accccggcat cagcaccatc cacggccggg aaattcccca caaagccgag tccggtcgta
    32581 ccatatggtt tgatttccgc gcactgtgct tcagtccccg ctcacagtcc gactatctgt
    32641 atttggccga acattatgaa atggttttta tttcaggttt ggaacaactc tcaccgcaag
    32701 aaaaggcgga ggcgcgacgg ctgacttggc tgattgacgt actctacgat ttccgggtca
    32761 aactgtgtgc caccggcgcg gtagatgtca accatatcta cacggaaggc gattttgccg
    32821 aagaatttac ccgcaccgcc agccggatgg tcgaaatgca gtccgaagtt tatttggaac
    32881 agccgcacct gaccttatct cccaaggctt caggcggata agttattttt ttggtagaat
    32941 accgatttga ttcttcttaa gtaaaaataa ggatatagca tggcgattga acgtaccata
    33001 tccatcatca aacccgatgc cgtcggcaaa aacgttatcg gcaaaatata cagccgcttt
    33061 gaggagaacg gtctgaaaat cgttgccgcc aaaatgaagc agcttaccct caaagaagcc
    33121 caagaatttt atgcggttca taaagaccgc cccttctatg ccggattggt tgaatttatg
    33181 accggcggtc cggttatgat tcaagtactg gaaggcgaaa acgccgttct gaaaaaccgc
    33241 gaactgatgg gggcgaccaa tcccaccgaa gccgcagaag gcacgatacg cgcggacttt
    33301 gccacttcgg tcagcattaa cgccgtacac ggttccgaca gtgtggaaaa tgccgctttg
    33361 gaaattgcct actttttcag ccaaaccgaa atctgccccc gttgatacaa tacaccgccc
    33421 aattcctatt cagacggcat aaaatatatc catgccgtct gaaaactctg ttgcaaaaga
    33481 cttcaaatca aacttgcctg ccctgcaatt ttttatttga agccttaatt taagaaaaac
    33541 acaaatacat gaaaaccaat ctgctcaact acgaccttca agggctaacc cgacattttg
    33601 ccgatatggg tgaaaaacca ttccgcgcca aacaggttat gcgttggatg caccaatccg
    33661 gcgcgcaaaa ttttgacgaa atgaccgatt tggcaaaatc gttgcgccat aaactgaacg
    33721 aacaggcaag catcgaaatt cccaagctga tgatgtccca agaatcttca gacggcactc
    33781 gaaaatggct tttggatgtc ggtacgggga acggcgtgga aaccgtcttc atccccgaat
    33841 cggatcgcgg cacgctctgc atttcctcgc aagtcggctg cgctttggaa tgtacatttt
    33901 gttcgaccgg ccggcagggc ttcaaccgca atttaaccgc tgccgaaatc atcggacagt
    33961 tgtggtgggc aaacaaagcg atgggcgtta caccgaaaaa cgagcgtgtg atttccaacg
    34021 tcgtcatgat gggcatgggc gagccgatgg cgaacttcga caatgtcgtt accgccttga
    34081 gcatcatgct ggacgaccac ggctacggtt tgagccgccg ccgcgtaacc gtttccactt
    34141 cgggtatggt tccccaaatg gacaggttgc gcgatgtcat gccggtggcc ttggcggttt
    34201 ccctccacgc ttccaatgac gaagtccgca accaaatcgt accgttgaac aaaaaatatc
    34261 ccttgaaaga attgatggcc gcatgccaac gctatctggt caaagcgccc agggatttca
    34321 tcactttcga gtatgtgatg ctggacggtg tcaacgataa ggcacaacat gcatacgaac
    34381 ttattgaact ggtcaaagat gttccctgca aattcaattt aatcccgttt aatcctttcc
    34441 ctaattcggg atatgaacgt tcaagtaacg aaaacatccg tattttcaga gatatcctgc
    34501 aacaagccga atttgtcgtt accgttcgaa aaactcgtgg cgacgatatc gatgccgcct
    34561 gcggacagtt ggcaggtcag gttcaggata aaacgcgccg ccaacaaaaa tggcagcaga
    34621 ttttaatcgg acaacagggg taattatgcc ttttaagcca tccaaacgaa tctctttatt
    34681 acttgttctt gccttgggcg cgtgcagcac ttcctaccgc ccctcgcggg cagaaaaagc
    34741 caatcaggtt tccaatatca aaacccagtt ggcgatggaa tatatgcgcg gtcaggacta
    34801 ccgtcaggca acggcaagta ttgaagatgc cttgaaatcg aaccctaaaa acgagcttgc
    34861 ctggctggtc cgtgccgaaa tctatcaata cctgaaagtt aacgacaagg cgcaggaaag
    34921 tttccggcaa gccctctcca tcaaacccga cagtgccgaa atcaacaaca actacggctg
    34981 gttcctgtgc ggcaggctca accgccctgc cgaatctatg gcatatttcg acaaagccct
    35041 ggccgacccc acctacccga ccccttatat tgccaacctg aataaaggta tatgcagcgc
    35101 aaaacagggg caattcggat tggcggaagc ctatttgaaa cgttccctcg ccgcccagcc
    35161 gcagttccca cccgcattta aagaactggc gcgcaccaaa atgctggccg ggcagttggg
    35221 cgatgccgat tactacttta aaaaatacca aagcagggta gaagtccttc aggccgatga
    35281 tttgctgcta ggctggaaaa ttgccaaagc cctcggcaac gtgcaggcgg catacgaata
    35341 tgaagcacaa ttgcaggcaa atttccccta ctcggaagaa ttgcaaaccg tcctcaccgg
    35401 tcaataaaca gattcaaacc atatgaacac actccaacgc cgcaagacgc atcaagtcct
    35461 catcgatcat atcaccgtcg gttcagaagc acccgtcgtt atccaatcta tgaccaacac
    35521 cgacactgcc gatgcaaaag ccaccgcatt gcagattaag gaattgagcg atgccggctc
    35581 ggaaatggtg cggattaccg tcaacagccc cgaagccgcg tccaaagttg ccgaaatccg
    35641 ccgccgcttg gacgatatgg gctatgccac accactgatt ggcgatttcc attttaacgg
    35701 cgaacgcctg ttggcggaat ttcccgaatg cggcaaagca ttgtccaaat accgcatcaa
    35761 tcccggcaat gtcggcaaag gcgtaaaagg cgatgaaaaa tttgccttta tgattcggac
    35821 tgctgctgaa aacgataaag ccgtccgtat cggcgtaaac tggggttctt tggatcagag
    35881 cctcgccaaa cgtatgatgg atgccaacct cgtttcttcc gcgccgaaac cgcccgaaga
    35941 agtgatgaag gaagcactga ttgtctccgc tttggaatct gccgaaaaag ccgttctatt
    36001 gggactgccc gaagacaaaa tcatcctgtc gtgcaaagtc agcgcagttc atgatttgat
    36061 tcaggtttac cgcgaactgg gcagccgttg cgtctatccg ctgcatttgg gtttgaccga
    36121 agccggtatg ggcagcaaag gcattgtcgc atcaacggcg gcattatctg ttttgcttca
    36181 agaaggaatt ggcgacacca tccgcatttc actcactccg gaaccgggca gcccgcgtac
    36241 tcaggaggtc gtcgtcgggc aagagatttt acagactatg ggtttgcgct cgtttacgcc
    36301 gatggttacc gcctgcccgg gatgcgggcg taccaccagt accgtatttc aagagctggc
    36361 acaagatgtt caaaattacc tgcgccaaaa aatgtctata tggcgtaccc tttatcccgg
    36421 ggttgaatcc ctgaacgttg ccgtaatggg ctgcgttgtc aatggccccg gagaaagcaa
    36481 attggccgac atcggaatca gccttcccgg tacgggggaa acacccgtcg cacctgttta
    36541 tgtagatggg gagcgcaaag tcacactgaa aggtaataat attgcaagcg aattcctagc
    36601 tattgtagag gaatatgtta aaaccaatta tggcaaaaac agttctaaac gcaataaagg
    36661 aaaagtcatc ccgatacagt ctctataaaa caaatgccgt ctgaacgttt cagacggcat
    36721 ttgtttctct cagatttctt attaaagccg caaagaagcg cggttttcca aaacttggtc
    36781 gatcaaacca tattcttttg cttcttcggc agacatgaaa ttatcacggt cggtgtcgcg
    36841 ctccaaatct gccaaatcgc ggccgcaatg tttcgccatc aggcggttga gtttttcttt
    36901 gattttcaac aactcgcgtg cgtggatttc aatgtcggat gcctgaccgc ccaagccgcc
    36961 gctgattaaa ggctggtgaa tcataatccg gctgttgggc agggcgaaac gtttgccttt
    37021 ctcgcctgcc gacaataaga acgcgcccat acttgccgcc tgccccaagc acaaagtcga
    37081 tacatcgggc ttgatgaaat tcatcgtgtc gtaaatcgac ataccggccg ttaccgagcc
    37141 gccgggggaa ttgatgtaga agaaaatatc cttatccgga ttctcacttt ccaaaaacaa
    37201 cagttgggcg accaccagat tagcagactc atcggttacc gggccaacca gaaatacgat
    37261 gcgctctttc aaaagccggg aatagatatc gaatgcacgc tcaccgcgac cgctctgctc
    37321 gataacggta gggacaagat ggttatcaaa agacatttcg tctcctttca tgatggaaaa
    37381 gcaccaaagc gggctttaaa aacggcttcg gtgctttcaa aaactgcctt cagacagcat
    37441 tttcagaaca atcaggcttg cgcgcccatc acttcatcaa aagacaaagc ttttttgttt
    37501 actttggctt tgcccaaaac gaaatcaaca acgttgcttt ctactgccaa agaagtcggg
    37561 gcttgcaggc gggaagtatc tgcgtagtac cagtcaatca cttcttgagg atcttcgtag
    37621 ctttctgcga agttggcgac aacggctttg atttgctctt cagtcggttc cagtttgttt
    37681 tcgtcaacca gtttggccaa aatcagaccc aaagatacgc gacgttcggc ttgttctttg
    37741 aacatatcca aaggcaaatc caagttcgcg gcatcggtca taccttggtt aacgaagttt
    37801 tgtttcattt cgtttgccag gcgggcggct tcttcattga ccaaagcaac cggaactttc
    37861 aactctacgg ctttaatcag cgcgttcatt acggattctt tggtttgttc gttcacgcgg
    37921 cgttccactt cgcggcttac gtttttcttc acttcttcac gcattttggc aacatcgcca
    37981 tccgcaatac ccaaggcttt tgcaaaatct gcatcgactt caggcagagt gggctcggaa
    38041 acattgttca gcgtaatggt gaacacggca gttttaccgg caacatcttt gccgtggtat
    38101 tcttcaggga agttgacggt aacgtcttta ctttcgcccg ctttcatgcc gactacgccg
    38161 gcttcaaatt caggcagcat ttgacctgcg cccaatacga aggcgtagtt tttggatgcg
    38221 ccgccggcaa aaggttcgcc gtcgattttg ccttcaaagt cgatgatgac gcggtcgccg
    38281 tttcgggctt cgcggtcaac atggttgaag cgggtacgtt gtttacgcag gatttctaca
    38341 gtttggtcga cttcggcatc gccgacggaa gcggttactt tttcgacctc ttgtgcagac
    38401 aaatcgccga taacgacttc ggggaacact tcaaaaatgg cggcaacttt gaaagactct
    38461 ttatcgtctt gttcttcaac gccttcaaaa cgtggatagc ctgccacttt caactcttgg
    38521 gcaacggcaa catcgtagaa gcggcgttgc accagctcgt tgatcacgtc gttttgcgcg
    38581 ctcgcaccgt acatttgggc aatcattttt aaaggtgctt tacccggacg gaaaccgtcg
    38641 atttttgcac ggcgttgggt ttgtttcagt tttttatcgg tttctgcgtt gatttcggac
    38701 caaggtaggg acaacactac tttgcgttcc agattttcta aaatttcaac agttacgctc
    38761 atcataagcc cttaaatttg ttgtgttgat aaaatgataa accttcttcc ctgcatgggg
    38821 aagcaaacag cgcaacggta cgatatttga accgcattgc cgcaaagggg aaattttagc
    38881 tggcaagtat atcacaatgt ttcgcctgaa acataatatg ccgagacctt tgcaaaattc
    38941 ccctaaaatc ccctaaattt ccaccaaggc atttagggga tttcccatga gcaccttctt
    39001 ccggcaaacc gcccaagcca tgactgccaa acacatcggc cgcttcccgc tatcggagtt
    39061 ggaccaggtg attgattggc agccgatcga acaatacctg a
//
LOCUS       DAXFHF010000013        37846 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXFHF010000013 DAXFHF010000000
VERSION     DAXFHF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37846
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.013"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            42..115
                     /locus_tag="ACFIRM_000560"
     tRNA            42..115
                     /locus_tag="ACFIRM_000560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Cys,seq:gca)
     gene            216..305
                     /locus_tag="ACFIRM_000561"
     tRNA            216..305
                     /locus_tag="ACFIRM_000561"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Leu,seq:taa)
     gene            409..1461
                     /gene="sohB"
                     /locus_tag="ACFIRM_000562"
     CDS             409..1461
                     /gene="sohB"
                     /locus_tag="ACFIRM_000562"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC3028252.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(1512..2378)
                     /locus_tag="ACFIRM_000563"
     CDS             complement(1512..2378)
                     /locus_tag="ACFIRM_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC3028253.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(2389..2820)
                     /locus_tag="ACFIRM_000564"
     CDS             complement(2389..2820)
                     /locus_tag="ACFIRM_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC3028254.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(3055..3924)
                     /locus_tag="ACFIRM_000565"
     CDS             complement(3055..3924)
                     /locus_tag="ACFIRM_000565"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC3028255.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(4000..4911)
                     /gene="hisB"
                     /locus_tag="ACFIRM_000566"
     CDS             complement(4000..4911)
                     /gene="hisB"
                     /locus_tag="ACFIRM_000566"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC3028256.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(4928..6007)
                     /gene="hisC"
                     /locus_tag="ACFIRM_000567"
     CDS             complement(4928..6007)
                     /gene="hisC"
                     /locus_tag="ACFIRM_000567"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC3028257.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(6053..7342)
                     /gene="hisD"
                     /locus_tag="ACFIRM_000568"
     CDS             complement(6053..7342)
                     /gene="hisD"
                     /locus_tag="ACFIRM_000568"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC3028258.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(7339..8226)
                     /locus_tag="ACFIRM_000569"
     CDS             complement(7339..8226)
                     /locus_tag="ACFIRM_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC3028259.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFYNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(8318..8983)
                     /gene="hisG"
                     /locus_tag="ACFIRM_000570"
     CDS             complement(8318..8983)
                     /gene="hisG"
                     /locus_tag="ACFIRM_000570"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC3028260.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(9062..9715)
                     /locus_tag="ACFIRM_000571"
     CDS             complement(9062..9715)
                     /locus_tag="ACFIRM_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC3028261.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            9991..10075
                     /locus_tag="ACFIRM_000572"
     tRNA            9991..10075
                     /locus_tag="ACFIRM_000572"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10025..10027,aa:Leu,seq:caa)
     gene            complement(10138..10245)
                     /locus_tag="ACFIRM_000573"
                     /pseudo
     CDS             complement(10138..10245)
                     /locus_tag="ACFIRM_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10779..12506
                     /gene="ilvB"
                     /locus_tag="ACFIRM_000574"
     CDS             10779..12506
                     /gene="ilvB"
                     /locus_tag="ACFIRM_000574"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC3028262.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            12517..13008
                     /gene="ilvN"
                     /locus_tag="ACFIRM_000575"
     CDS             12517..13008
                     /gene="ilvN"
                     /locus_tag="ACFIRM_000575"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC3028263.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            13075..13368
                     /locus_tag="ACFIRM_000576"
     CDS             13075..13368
                     /locus_tag="ACFIRM_000576"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC3028264.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            13448..14461
                     /gene="ilvC"
                     /locus_tag="ACFIRM_000577"
     CDS             13448..14461
                     /gene="ilvC"
                     /locus_tag="ACFIRM_000577"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC3028265.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(14580..15575)
                     /locus_tag="ACFIRM_000578"
     CDS             complement(14580..15575)
                     /locus_tag="ACFIRM_000578"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC3028266.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTSFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(15717..18302)
                     /gene="acnB"
                     /locus_tag="ACFIRM_000579"
     CDS             complement(15717..18302)
                     /gene="acnB"
                     /locus_tag="ACFIRM_000579"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC3028267.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPKAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            complement(18451..18612)
                     /locus_tag="ACFIRM_000580"
     CDS             complement(18451..18612)
                     /locus_tag="ACFIRM_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028268.1"
                     /translation="MPSEPLFRRHLFSLTHSRRHIAVPYYSGLNLNRYGVASPCRTIC
                     TVCGFVALS"
     gene            complement(18617..19687)
                     /gene="lptG"
                     /locus_tag="ACFIRM_000581"
     CDS             complement(18617..19687)
                     /gene="lptG"
                     /locus_tag="ACFIRM_000581"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC3028269.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(19684..20799)
                     /gene="lptF"
                     /locus_tag="ACFIRM_000582"
     CDS             complement(19684..20799)
                     /gene="lptF"
                     /locus_tag="ACFIRM_000582"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC3028270.1"
                     /translation="MIYQRNLIKELSFTAVGIFVALLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            20788..20970
                     /locus_tag="ACFIRM_000583"
     CDS             20788..20970
                     /locus_tag="ACFIRM_000583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028271.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            20973..22379
                     /locus_tag="ACFIRM_000584"
     CDS             20973..22379
                     /locus_tag="ACFIRM_000584"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC3028272.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            22442..22882
                     /locus_tag="ACFIRM_000585"
     CDS             22442..22882
                     /locus_tag="ACFIRM_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC3028273.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            23142..23960
                     /locus_tag="ACFIRM_000586"
     CDS             23142..23960
                     /locus_tag="ACFIRM_000586"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3028274.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            24067..24693
                     /gene="purN"
                     /locus_tag="ACFIRM_000587"
     CDS             24067..24693
                     /gene="purN"
                     /locus_tag="ACFIRM_000587"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC3028275.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADVAAGRLIIEGNRVRNSENADAARFLTA"
     gene            24709..25380
                     /locus_tag="ACFIRM_000588"
     CDS             24709..25380
                     /locus_tag="ACFIRM_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC3028276.1"
                     /translation="MMKTFKNIFSAAILSATLPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            25498..25920
                     /locus_tag="ACFIRM_000589"
     CDS             25498..25920
                     /locus_tag="ACFIRM_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC3028277.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            26441..27157
                     /locus_tag="ACFIRM_000590"
     CDS             26441..27157
                     /locus_tag="ACFIRM_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC3028278.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGSCMERLEKTLED"
     gene            27306..28208
                     /locus_tag="ACFIRM_000591"
     CDS             27306..28208
                     /locus_tag="ACFIRM_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC3028279.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            28251..29024
                     /locus_tag="ACFIRM_000592"
     CDS             28251..29024
                     /locus_tag="ACFIRM_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC3028280.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRCDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAAASELVKRRSSLRIITNNIHVASVVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDYDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGGITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            29136..30824
                     /locus_tag="ACFIRM_000593"
     CDS             29136..30824
                     /locus_tag="ACFIRM_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC3028281.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPALHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            30916..31872
                     /gene="gshB"
                     /locus_tag="ACFIRM_000594"
     CDS             30916..31872
                     /gene="gshB"
                     /locus_tag="ACFIRM_000594"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC3028282.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKEFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            32031..32414
                     /locus_tag="ACFIRM_000595"
     CDS             32031..32414
                     /locus_tag="ACFIRM_000595"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC3028283.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            32546..33019
                     /locus_tag="ACFIRM_000596"
     CDS             32546..33019
                     /locus_tag="ACFIRM_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC3028284.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMSAMNHGHHHGEAHQH"
     gene            33144..34247
                     /gene="mnmA"
                     /locus_tag="ACFIRM_000597"
     CDS             33144..34247
                     /gene="mnmA"
                     /locus_tag="ACFIRM_000597"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC3028285.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            34318..35988
                     /locus_tag="ACFIRM_000598"
     CDS             34318..35988
                     /locus_tag="ACFIRM_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC3028286.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTQEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            36100..37734
                     /locus_tag="ACFIRM_000599"
     CDS             36100..37734
                     /locus_tag="ACFIRM_000599"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC3028287.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         8914 a         9684 c        10381 g         8867 t
ORIGIN      
        1 ctcgtttccc gctccaagtt ttgtagacaa gccagtttta gggcgagata gcaaagtggt
       61 tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc cgactctcgc ctccatatat
      121 cttacggcgg ggtggcagag tgtttatgct atgaggggtg caaccttcat ataggccggt
      181 taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa taggtagaca
      241 caacggactt aaaatccgtc gggactaaac atcccatgcc ggttcgattc cggctccggg
      301 caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg tcggatgggg
      361 atggacaata ttgatatgtt catgcctgaa caagaggaaa tccaatcaat gtggaaagaa
      421 attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg cgcaattgcg
      481 ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag tgtcgtactg
      541 acagattttt cggaaaatta taaaaaacag cggcaatcgt ttgaaacatt ctttttaagc
      601 gaggaagaga caaaacatca ggaaaaagaa gaaaagaaaa aggaaaaggc ggaagccaaa
      661 gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg ccgaaacgca aaaatcccgc
      721 ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc cttgcgtcat
      781 gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct cagattggaa
      841 agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag gcgtttgcgc
      901 gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg cggcaagcgg cggctatatg
      961 atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattatcgg ttcggtgggt
     1021 gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa aacatgatat tgatgtggat
     1081 gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa tacggaaaag
     1141 ggcaaacaga aattccggca ggaactggag gaaacgcatc agttgttcaa gcagtttgtc
     1201 agtgaaaacc gccccgggtt ggatattgaa aaaatagcga cgggcgagca ttggttcggc
     1261 cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga tttgttgttg
     1321 aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagcg aagcctgatc
     1381 cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt tgtttgccaa acttgtcaac
     1441 cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga agtgtgttca
     1501 gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat ccgttgtccg
     1561 atgtctttca aattggaata ttgttcgacc aatcgcggca catcgtccaa actcatggca
     1621 aacatccaca gataagtgcc gaccgaataa atatgccccg cgctgccgta gcctttgagc
     1681 gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac gcagagatag
     1741 ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg aaaccagtcc gtaatggcgg
     1801 tacagctgcc gctcgtcgcc ttttcggata aagtggttgt cgcgttccaa gctgttgttc
     1861 aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca taaaaacaac
     1921 gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag catgatgcac
     1981 gcgccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc aaaaaagctg
     2041 acaaattcac gcgacagggc aacccgtgca gttaccgctg aatgcgggac ttgccgctgc
     2101 cgttgttcca acacaaccgg cacggcgatt tcggtataaa tccgcgtaaa cgtgcgcgta
     2161 tcggcaatcc gccgtgccgc accgaccagc cacatcaaaa atacaaccaa agcgtacagc
     2221 aacgcctgcc acaccctccc cgcaatcacg gcattaatcg cccagccgcc aaacaccgga
     2281 taccccagca tcaaaaggtt ttccagtccg accggggaaa atgtgccaat caatcgcttg
     2341 cggtgggttt tggctatgtg tttcaacatt ttccacatat ttgtccctct attttttatt
     2401 ttccgagatt gttttttcca tcacttccgc cagtgcatcc aaatcggcaa acagccgggt
     2461 tgtgcgcttg tcgccgaatg tggaaaatac tttgtcgctg aattcctgcg cgttttctgt
     2521 taaaggtgcg gcatggactt tgcctttttc ggtcagcgac agcaaccgtt cgcgcctgtc
     2581 ctgttcgcct tcctgccatt caatcaaccc ttgtccggca agggttttgc atacgcctga
     2641 aacggtctgt ttgggcagac tccactcttc tccgatgtgc ttttgcgtgc ggctgccttc
     2701 ggttgccagg gtataaagta cggtaaagag gttgtaattc aaatcctgct gcccgatcca
     2761 tttgtcaaag acattgcaaa tcaggttgat acggattcca agttggtcga gtcggttcat
     2821 aattggtctt gatattgact aaatttgtgc ggattatagt ggattaacaa aaaccggtac
     2881 ggcgttgcct cgccttgccg tactatctgt actgtcttcg gcttcgtcgc cttgtcctga
     2941 tttttgttaa tccactatat agtcataatc gggactgatt tcaagcgtgg ggcgataaaa
     3001 atgccgtctg aaacgctttc tggcttcaga cggcatattg tttggaaagg gaagtcagtg
     3061 ttctgccaat ttcaggtaaa cgccggaaac gtcctgttcg ccgtagccgg cttcaactgc
     3121 tttgcggtag ctggcagcaa cggtttcgac ggcgggcagg gtgttgcctg cctgttcaag
     3181 ctctttgacg gcgaggttaa ggtctttgga agcgtgtttg agtgcaaagg caggggggaa
     3241 ctcacggttt gcccatagtg atttttttgt ttgaaacata ggcgagtcca ttgccgagcc
     3301 gccgatggct tcgacgatgg tgtcggtatc gatgccgaac tgccgcgcca tcagcatcgc
     3361 ttcgctgtac gcttcgccga aaatgcctaa gagcgagttc aagacgagtt tcgcgcccga
     3421 gcctttgccg acatcgccga aatggaaggt ttttttgccg acaagggaaa atattttttg
     3481 cagcgggttt aaaacggctt cgctgccgcc gaacagaatc agcagtgtgc cgttggtggc
     3541 gggtccgacc gatccggaaa cgggtgcttc ggcaaactgt ccgcctgcgg cttcgacaag
     3601 tgctttgacg gcgaggtttt cggtcgggga gatggtgctc atgttgacga tgattttgcc
     3661 ggccaatccg tcgcggactc cgttcaggat gtcgcacacg gcggcatagt cggaaaccat
     3721 caggaaaatg acgggacagg cgcggacgag ttcggcggtg ctgccgtaaa cttttgctcc
     3781 tttggcggag atgggggcag ttttgtcggg cgagcggttg tatacgccga cttcgatgcc
     3841 gccgtccaag agccgcgtta ccataggcag acccatttgc cctaagccta cccagccgat
     3901 ttgtgtgtat gtttctgcgg acatagtgtt tctcctttgt tggggcgctg taatgccgtc
     3961 tgaaggcttc ctgcgcttca gacggtctgt ttggggatgt tatgcggtca gcgtgccttt
     4021 ggtcgagggg gtttgcccag ccattctcgg gtcgtgttcg actgccatac gcagggcgcg
     4081 cccgaaggct ttgaatacgg tttccgcctg atggtgggcg tttttgccgc tgaggttgtc
     4141 gatgtgcagg gtcatcatgc tgtggttgac gatgccgtgg aaaaattctt caaacaaatc
     4201 gacatcgaaa cgtccgatta gtgcgcgggt aaattcgatg ttgtacacga gtccggggcg
     4261 gccggaaagg tcgatgacga cgcggctgag ggcttcgtcg agcgggacgt aggaatgtcc
     4321 gtaacggcgg atgccttttt tgtcgccgag tgcctgccgg attgcttgtc cgagtgtgat
     4381 gccgatgtct tcggcggtgt ggtggtcgtc gatgtgcagg tcgcctttgc agctgatgtc
     4441 gatgtcaatc atgccgtggc gggcgatttg gtcgatcatg tgttcgagga agggaacgcc
     4501 ggtatccagc ctgcttttgc cgctgccgtc gaggttgatg gagacggtga tttgggtttc
     4561 gcaggtattg cggttgacgg taacgctgcg gcagccggca gcagatgcgg tttcggcaat
     4621 tcgggtttcg gcagttccgg tgctttctgc ggcggtttcg gggacagtgc gttcgcggtg
     4681 ttttctgtcg agccagcctt tgggtttgcc ggtgtgtttt tcgagttttg ccatcaggct
     4741 gggacggatg ccgcgcgcat cggggtcttc tgcctgcttt tcaaggcgtt gtttgagttt
     4801 gtaaagtgcg acgggggtac ggtagccgca gagtttggca agcttgggca gcgaacctgc
     4861 ttcttgggcg agagtcagga agttgttcag gtggagttgt gtcttggtca tagggattcc
     4921 gtttggttta tcggtagagc cggcggataa cgtcgaggac ggcatcgttt tgtgcggagc
     4981 tgcctatggt aatgcgcagg cagtgttcca aaagcggatg tgcgccgtgc agttttttga
     5041 cgaggatgcg gttttgtttg agtgtgtcaa acaacaaatc ggcatcgggt acgcgtatgg
     5101 taatgaagtt tgcctgactt ggaaaggcgt tcagacggca tattttgccc aattcggcga
     5161 acatccgttc gcgttcgttt ttcaggctgt cgatgttggc agaaatgatg ccgtagtgtt
     5221 gcagggcgag tttggcggtg gtcaggctca gttggttcat attgtagggc ggcaggattt
     5281 tttgcagttc gccgatgact tcgggacagc ctgtcgcata accgatacgc agtccggcaa
     5341 aaccgatttt gctgagggtg cgtaagacta tcaggttggg aatcctgccc gcctgcggca
     5401 ggaagctgtc gccgttgaat gcgccgtagg cttcgtcgac gacgacgatg ccgtctgaag
     5461 cttcgatggc ggcttcgatt tcggcacgcg tgaaacatac gccggtcggg ttgttcgggt
     5521 aggcgataaa ggtcagggca gggcggtgct tcctgacggc ttcgaggacg gcgggcaggt
     5581 tgagggtgaa atctccgttc agtggaacgc cgacataatc catgccgtac agcgcggcgt
     5641 tgtggcggta catgatgaaa ccgggttcgg ctgccaacat tgccgcgccg ggtttggcgg
     5701 tcagcatggt gatgaactgt atcagttcgt ccgaaccgtt gcctaaggcg acggcggcgc
     5761 agtcagggat gtcgaacgcc gaacgcaatg cttcctgcaa accgcagccg gaggggttgg
     5821 gatagaggtg gatgggggcg gatgccagtt gcgcccgcca ttcccgcatc agggcttcgt
     5881 gcccctcaaa aggatgggcg gggctttcca tcgcatcgag tttggcaaaa ccgggcggaa
     5941 catcggtgat tttgtatgcg gacatggcga ggatgtcgtt gcggatgaag gagcggacgg
     6001 atttcatcgt gtttccttaa tggttggaat atgcctgtac gccgtttcgg cattatttca
     6061 tacggaactc tgccgcgcgt gcgtgggcgg tcaggctttc gccgtgtgcc agcacgctgg
     6121 cggtttcgcc taatttttgc gcgccctgtt ccgaaacctg aatcaggctg gagcgttttt
     6181 ggaaatcata tgtccccaaa ggcgaggaaa agcgggcggt tcggctggtg ggcaacacat
     6241 ggttcgggcc ggcgcagtag tcgccgaggc tttcgccggt gtagcgtccc atgaaaatcg
     6301 cgccggcatg gcggattttt ttcgcccatt cctgcgggtt ttcgactgac agttccaagt
     6361 gttcggggga aatgtagttg gagatttcgc aggcttcgtt caagtcttta acgagtatca
     6421 tcgcgcccct gttgccgagc gaggcctcga tgatgtcgcg gcgcggcatg gtttcgatga
     6481 gcctgtccat agcggcttct acttcgtcga gatacgcttg cgacgtgccg atgaggatgg
     6541 cttgggcaat ttcgtcgtgt tcggcctggc tgaacaaatc catcgccacc caatcggcgg
     6601 gtgtcgtgcc gtcggcgatg accagtattt cagacggccc cgccaccatg tcgatgccga
     6661 ccacgccgaa cacgcggcgt ttggcggcgg cgacgaaggc gttgcccgga ccggtgattt
     6721 tatcgacttg ggggatggtt tccgtgccgt aggcgagggc ggcgatggct tgcgcgccgc
     6781 cgacggtgaa gactttggtt acgccggcga cgtatgcggc ggcaagcacg atgtcgttgc
     6841 gttcgccttt cggtgtcggt acgaccataa tgatttcttt cacgcctgcg acgtgggcgg
     6901 gcatcgcgtt catgatgacg gaactcggat atgccgcctt gccgcctggg acgtaaatgc
     6961 cgacgcggtc aagcggggta atctgttgcc ccagcagcgt gccgtcttca tcggtgtagc
     7021 tccacgattc cattttttgg cgttggtggt agctttcgac acggcgggcg gcggtctgca
     7081 atgctgtctg aacgtcgttc ggaatgcgct cgaacgccgc gttcaaatcg gcttgcgtga
     7141 gtattaaatc atcgatgctt ttagcgtttg tctgatcgaa tttgttggtg tattcaatca
     7201 aagccgcatc gccgcgcttt tgcacgtcgg cgcaaatgtc ggcgacgatg cgttcggttt
     7261 cggggttttg cgcggtttca aaagccagca gggctttgag tccggcttgg aaatcgggcg
     7321 attgggtgtt gagttttttc atgatttggg attctccttt gttattttgg gacttgggat
     7381 cgtctgaagc gtctcttgct cggttttggg tctgatatac gcttttcctc ccccgctacc
     7441 aaacttcaat tcgggcaggc atttttgttg cagaccgagt ttggctaata cctcttcgct
     7501 acgagaggta gtacggaaac ggtaacgggt ttgttttctg ccgtttttgg tttcctcagt
     7561 cattgtttca aaaccgtcca cttcgtacgc actgacaaca gcgttttgca gaccggtgtg
     7621 aacgccgata acgtatttca cttttgatgc aacatcttta ccgataaccc agttgcccaa
     7681 cgtacgcgat ttgaggttgg catcgtcttg gttgtagaaa aggtagtcta attcttcgtc
     7741 agtatctaaa tcgaaagcat tgtggatctt gatggcgaga atcagcccgt cggggttaat
     7801 ctcgctaagt gggagagaag agaatccaaa gcggcgatct agttcttcta cgctaatacc
     7861 acccggacca tgcccggcaa ttttattttt gagtttttta cccaagacag atttaacaaa
     7921 atgaattaag gcagattcgg cggcgagcgc ttcgacttca gtcagatgat agctgatgat
     7981 atagcgaccg agtttcttgc atttggagat ggctttgagt ttccgatcga taatctcgcc
     8041 ggagactggg tcttgtgaac gactagcaac ccattcatgc tcgaagatac gattaccgca
     8101 accttttccg atgtagagga ttttgtcttt tttcaggtcg gtcagacaat agacataaaa
     8161 ccgacgctcg ccgttattca aaacggaaag ggtcgaatct gagaattttt ttatttttgc
     8221 aaccatttta acttctccta tacagtttac tgccggtgat attttcagac ggcctgtcgg
     8281 cgggaacgga tagggtcgtc tgaaaacgca tctttattca aatgaatgcc cacttcgcct
     8341 tcactgcgcc gccgaacgac tgaataatcg gctccagcag cgcgtatttc gttttcaaag
     8401 cagccttgtt gaccaccagg tagctggaaa tgtcgacgat gtgttcgact gcctccaagc
     8461 cgtttgcctt caaggtgttt cccgtcgaaa ccaagtccac aatcgcatcg ctcaagccga
     8521 ccagcggcgc aagttccatc gagccgtaca gtttgataat gtccacatgg acacccttgc
     8581 cggcaaaatg agatgccgcg atttcaggat acttcgtggc aatcttcaga cggcatccgg
     8641 gttgcgaagc ggcttcgtaa tcaaaccctt tacgcacagc aaccatcatg cggcacttgg
     8701 caatctccaa atccaaaggt cggtaaagcc ccgtgccgcc gtgttcgatc agcacgtctt
     8761 tgcccgcaat gccgaagtcc gccgcgccgt agcggacata agtcggcaca tcggttgcgc
     8821 ggacaatgac aaggcggatg ttttcatggt tcgtcccgat aatcagcttg cgcgattttt
     8881 cgggctcttc agtcggagca atgccggcag cggcaagcag cggcagcgtc tcctcaaaaa
     8941 tgcgcccttt ggataaggcg atggtcaaag cattatcctg catggtgtgg atgtcctttg
     9001 tcggttgaaa caataatgcc gtctgaacag gcaccggcgg tgcagacggc atacggtttt
     9061 atcagagcag ggtccttaca tcggcagcaa tcgtttccgg ctcgcttccg taaggcgaga
     9121 aaatggcaac ctcaccgttt ttgtcgataa gatacgcacc ggaagagtgg tcgaccaaat
     9181 agttttcgct gtcgtctttt tgattgattt tggcagaaac cacgcggtat tgctgcttga
     9241 tgaccggcag gttttggccg cccgttgccg tcagaccgat aaagtccgga ttgaactgtt
     9301 tggcatactt gccgatgatt tcaggcgtgt cgcgttccgg atcgatgctg acgaacacca
     9361 ctttcacgtc cttagcctgc ccgcccaact gcttcaaagt gtcgctgtac gtcaaaagct
     9421 ctgtcgggca gacatcggga cagtgcgtaa agccgaaaga cagaatcacg accttgcctt
     9481 tcaaatcgct caggctgaaa ggcttgcctt cgccgtcggt cagcgtgaaa tcgccgccga
     9541 tgtcttcctt acgcatatcc gtaccgcgcg tttgcggctt tgccgcattt tccgcagccg
     9601 gcgcggatgc acttgaagaa gcggcttgcg ccgcactgtt gtcttgaggt ttgcaggcgg
     9661 caagcgcggc aagtacgaaa acgcccggca aaaaggaacg cggtacggaa aacatgacaa
     9721 tctcccaaga ataaatagaa aaacctgatt cgtacggaat ttgaatattt ccatcttaac
     9781 gcgtttggca gatttaatgt agtatttttt tacgttgtcc gtacggcagg aattgccgaa
     9841 tcatacagaa tctttggagc ggaaagaaca gtaagattaa aataataaaa attctattta
     9901 aaatcaaaaa caaaataaaa acagataaaa taatttcaaa aaaaggttta cagaatctca
     9961 aaatccatta taatggcaat ttctttacca gcccaggtgg cgaaattggt agacgcaggg
    10021 gactcaaaat cccccgccgc aaggtgtgtc ggttcgagtc cgaccctggg caccacaacc
    10081 gcttttggag cggttatttt tttgtctatt gctgtttaat gaaatagata agactaaata
    10141 tagtggatta aatttaaacc ggtacggcgt tgcctcgcct tgccgtacca tctgtactgt
    10201 ctgcggcttc gtcgttttgt cctgatttaa atttaatcca ctatacaatc aatatattta
    10261 agtcgttgtt agccctcttg gttctgtcaa tgccgtccaa tacagtccgg tgcaatgagc
    10321 cattttcagg aagtatggtt gggaatattt ttgatatatc aaaaataaaa tcaagttcaa
    10381 tcaatatccc gagtgaaacc ctgtaaattt taaaattctt gatactttca catctgcccc
    10441 catgcaaact gtcaacaatt tccatctttc aaaaaaggta ctcctgattt tattctttta
    10501 aaatcaatca ataatttctc cgctttcaaa aaaatttctc cgctttcaaa aaatattaaa
    10561 agatgaaaga tttttcttga ttgaactgtc ggtttgcgtt atcgttttta ctctttttca
    10621 caaactctgt gttcctttcc aattgattgg ataatctgtc tgttgccgtg ttcccccaat
    10681 gcgcgtaccc taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg caaagcggtt
    10741 tgtccctatt ggtttgaaac cgtataaaag aggtcgttat gcagttatca ggcgcgcaaa
    10801 tcatagtgca gagtctcaaa gccgaaggcg tcgagtacgt tttcggttat cccggcggtg
    10861 cggttatcga aatctacgat gccctgttcc aactcaataa attcaaacac attcttaccc
    10921 gccatgagca ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc ggtaaggtgg
    10981 gcgtggcatt ggttacatcc ggcccgggcg ttaccaatgc gctgaccggt attgctactg
    11041 cctatacgga ttcgattccg atggtggtca tcagcggaca ggtaggcaat tccctgattg
    11101 gtacggatgc gttccaagaa gttgatacgg tgggtattac ccgtccgtgc gtcaaacaca
    11161 atttcttggt tacggacatc aatgagttgg tggaaaccat taaaaaggcg ttccaaattg
    11221 ccgcaagcgg ccgaccgggg cccgtggtgc ttgatgtccc gaaagatgtt acgcaggcga
    11281 tggcgaaatt cagctatcct caggaagaca tttttatccg ttcgtatcaa ccggttgttc
    11341 aagggcatat cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc aaacgcccgg
    11401 tcgtctattt tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg acccgatttg
    11461 tccgaatgac gggtgctccg tgcacgggta cgctgatggg cttgggcgct tatccttccg
    11521 gcgaccgcca attcctcggt atgctcggta tgcacggtac ttacgaggca aaccttgcca
    11581 tgcagaatgc ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt gtggtttccg
    11641 taccgtccaa atttttcgag aaggcgaaga aggttatcca tatcgatgtc gatccttcca
    11701 gcatcgccaa acgcgtgaag gcggacattc cgattgtcgg cgacgtgaaa aacattttgt
    11761 ccgagatggt tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat gctttgggca
    11821 aatggtggaa aaccatagag gaatggcgtt cccgcgattg cttgtggttt gacaacggca
    11881 gcgaaattat caagccccaa tatgtgattc agaagcttgc cgagattacc ggcaattcgg
    11941 caatcatcac atcggatgta gggcagcatc aaatgtttgc ggctcaatat tatcctttcg
    12001 aacgtccgcg ccaatggctc aactccggcg gtttgggtac gatgggcgtg ggtctgcctt
    12061 atgcgattgg tgcaaaactt gccgccccgg atcaagacgt attctgtatt accggcgacg
    12121 gctcgatcca gatgaacatc caagagttgt ccacctgttt ccaatatcgg attccggtta
    12181 acgtcattac gctgaacaac ggttatctgg gtatggtacg ccagtggcag gaaatatatt
    12241 acggcggtcg agagtcggaa acctatttcg attctttgcc cgatttcgtc aaacttgccg
    12301 aggcatacgg ccatatcggc atccgcgtgg acaagaaatc tgatgtggaa ggtgccttgt
    12361 tggaagcatt gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc gaccagaaac
    12421 agaatgtgat gcctatggtc ggcaacggca aaggtttgga cgaaatggta cttccgccgc
    12481 atatgcgtac ggacggaaag gcgtaaggag aggcaaatgc gacatatctt atctgttctg
    12541 atcgaaaacg aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc acgcgattac
    12601 aatatcgatt ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg gatgaccatc
    12661 gttacccacg gcgacgagca ggttatcgaa caaatcacca agcaactcaa taaattgatt
    12721 gaagtgatta aagtggtcga tttgaacgaa agccgttttg tcgaacgcga actgatgttg
    12781 gtaaaagtcc gtgccgccgg caaagaccgc gacgaatttt tacgcttgac cgaaatctac
    12841 cggggcagca tcatcgacgt aaccgaccgc agctatacga ttgaaattac cggctcgacc
    12901 gacaagctgg attccttttt ggaaacggtc ggacgcgccc aaattttgga aaccgtacgt
    12961 acaggtgcgg ccggcatcgg tcgcggtgag cgtattttga aaatttaacg ccgtaacctt
    13021 tcagacggca tggtatttga atgccgtctg aaaaacgaac ggcaggagag atttatgtcg
    13081 aacattaaaa ttgtcgcact ggttaccgtc aaaccggaat acacggaaac actggcagca
    13141 cagtttaaag aactggtcaa agccagccgt gcggaagagg gcaacatcag ctacaatctc
    13201 catcaggaaa tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa atcccaagca
    13261 gctattgacg aacataatgc cagcgcgcat ttccaagcct tcgtccagtc cgtcgacggc
    13321 aaaaccgaag cgttggaaat cgtattgatg aatgaagttg ccgtttaagc ggcacactct
    13381 gtttaacccg tccgaagccg ctcaacattt taaggcttcg acaaccattt accttaaagg
    13441 aaatcaaatg caagtctatt acgataaaga tgccgatctg tccctgatca aaggcaaaac
    13501 cgttgccatc atcggttacg gttcgcaagg tcatgcccat gctgccaacc tgaaagattc
    13561 gggtgtaaac gtggtgatcg gtctgcgcca cggctcttct tggaaaaaag ccgaagcagc
    13621 cggccatgtt gttaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg ttatgctgct
    13681 gctgcctgac gaaaccatgc ctgccgtcta tcacgccgaa gttgcagcca atttgaaaga
    13741 aggcgcgacg ctggcatttg cacacggctt caacgtgcac tacaaccaaa tcgttccgcg
    13801 tgccgacttg gacgtgatta tggttgcccc caaaggtccg ggtcataccg tacgcagtga
    13861 atacaaacgc ggcggcggcg tgccttctct gattgccgtt taccaagaca attccggcaa
    13921 ggccaaagac atcgccctgt cttatgcggc tgccaacggc ggcaccaaag gcggtgtgat
    13981 tgaaaccact ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag ccgtattgtg
    14041 cggtggcgtg gccgagttga tcaaagcagg ttttgaaacc ctgaccgaag ccggttacgc
    14101 gcctgaaatg gcttacttcg aatgcctgca cgaaatgaaa ctgatcgttg acctgatttt
    14161 cgaaggcggt attgccaata tgaactactc catttccaac aatgcggagt acggcgaata
    14221 cgttaccggc cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca atgccctgaa
    14281 acgcatccaa accggcgaat acgcaaaaat gtttatccaa gagggtaatg tcaactacgc
    14341 gtctatgact gcccgccgcc gtttgaatgc cgaccaccaa gttgaaaaag tcggcgcacg
    14401 actgcgtgcc atgatgcctt ggattacggc caacaaattg gttgaccaag acaaaaactg
    14461 attgttttca aacgggactg cctacacatc gtgtaggcag tttgttatat ggataccgtc
    14521 tgaacatcgt gttcggacgg tatctgtgtt gcggatgaat ttaaacaggc acagttctgt
    14581 cagtcgccca gagccgcgac cattaccgct ttaatcgtgt gcatacggtt ttccgcctga
    14641 tcgaacacga tgccggccgg actttcgaat acttcttctg taacttccac accgttcagc
    14701 ccgaaggttt cgtaaatcca ttcgccgact ttggtttcgc ggttgtggaa ggcgggcagg
    14761 cagtgcatga atttgacttg cggattgccc gatgccgcca tcagttcggg cgtaacgcgg
    14821 taatctttca gcaaatcgat gcgttcctgc cagacttctt tcggttctcc catactgacc
    14881 catacgtcag tatgaatgaa accgacacct ttgacggctt catgcgcgtt ttcggtcagg
    14941 gtaatttttg caccggtttc tttggcggcg gcgtgtgcgg cggcgataat gccttcagac
    15001 ggccacaggc tttgcggtgc gccgatacgt acgtccatcc ccaattttgc ccctaaaatc
    15061 agcagggaat tccccatgtt gtaacgcgcg tcgccgacat aggcaaatga ggtttggttc
    15121 aaaggtttgc cgctatgttc gcgcatagtc agtgcatcgg caagcatttg agtggggtgg
    15181 aactcgttgg tcagaccgtt gaacacgggc acgcctgcat attttgccaa ttcttcaaca
    15241 acttcctgac caaagccgcg atattcgatg gcatcgtaca ttctgcccaa gacgcgcgcc
    15301 gtgtctttga tgctttcttt gtgcccgatt tggctggcag acggttccag ataggttgcg
    15361 cccgcgcctt gatcgcgtgc ggcaacttca aacgcgcaac gtgtgcgggt cgaagttttc
    15421 tcaaaaatca gggcaatgtt tttccctttc atccgttgga tttcgcgccc agcctttttg
    15481 gcggctttca attcggcggc aaggtcgagg taggcggtga tttcttccgg cgtgaagtcc
    15541 aaaagtttca gaaaatggcg gtttttcagg ttcactgtcg tttcctttat gtgggggcgt
    15601 gaacgccgta tttgcagcgt ttgcggaaag aagggttagc ggaatgttgc aatgccgtct
    15661 gaaggttgtt cagacggcat tgcggtcaat cccttactcc atacggatga cggggatcaa
    15721 acattcacgg tctcggctac ttcgttgtag ctgtcgattt cgttgaagtt catatagcgg
    15781 tagattttat cgccctgttc gttgatgatg ccgatattgg cttgatattc ttcaacggtc
    15841 gggattttac ccagtttgga gcaaatcgcc gccaactctg ccgagccgag gtaaacgaag
    15901 gtgtttttac ccaagcggtt cgggaagttg cgggtcgaag tggacatgac agttgcacct
    15961 tcgcgtactt gggcttggtt acccatacac aacgaacagc ccggcatttc catacgcgcg
    16021 ccggcgcggc cgagtacgcc gtagtgacct tcgtcggaca actctttcgc gtccattttg
    16081 gtcggcggcg ctacccacag gcggacgggg atgtcgctct tgccttccaa aagtttggag
    16141 gcggcgcgga agtggccgat gttggtcata caagaaccga tgaacacttc gtcgattttg
    16201 gtgccggagc gttcggacat aaagcatacg tcgtccgggt cgttcgggca ggcgataatc
    16261 ggctctttga tgtcgtccat gttgatttca atcacggcgg cgtattcggc atctttatcc
    16321 gcttcgagca actcgggatt tgccagccat ttttccatag ctttgatgcg gcgttccaaa
    16381 gcgcgcggat cttgatagcc gttggcaatc atatttttca tcaacacgac gttggatttc
    16441 atgtactcga taatcggctc tttattgagc ttcacggtac agccggcggc ggagcgttcg
    16501 gcggatgcgt cggtcaattc aaaggcttgt tccactttca aatcgggcag gccttcgatt
    16561 tcgaggatgc ggccggagaa gatgtttttc ttaccggctt tggcaacggt cagcaaacct
    16621 tgtttaatcg cgtaaagcgg gatggcgttc accaaatcgc gcagggttac gcccggttgc
    16681 agcttgccgc tgaaacgtac caatacggac tcgggcatat cgagcggtat tacgcccgtt
    16741 gcggcggcaa aagcgaccaa gcctgaacct gcggggaagg aaataccgat ggggaaacgg
    16801 gtgtggctgt cgccgcctgt accgactgta tcaggcaaca gcaggcggtt gagccacgag
    16861 tggatgacgc cgtcgcccgg acgcagggac acgccgccac gggtagaaat aaaggcgggc
    16921 agttctttat gggtttttac atcgacaggt ttcggatagg cagcggtgtg gcagaaagac
    16981 tgcatcacca tatcggcgga gaagcccaaa caagccaagt ctttcaactc gtcgcgggtc
    17041 atcggcccgg tcgtatcttg cgagccgacc gtcgtcatgc gcggttcgca gtaagtaccc
    17101 ggacgcacgc cttgtccttc gggcagaccg caggcgcgac cgaccatttt ttgcgccaag
    17161 gtgaaaccga ctttgctttc ggcaggcgct ttcggcaggc ggaatgcagt agaggcaggc
    17221 agtttcaggg cttcgcgcgc tttggcggtc agaccgcgac cgataatcag gttgatacgg
    17281 ccgccggctt gcacttcgtc cagcaatact tgtgatttca attcaaactc ggcaacagtc
    17341 tcgccgtttt tcacgatttt accttcataa ggaaggatat tgacgacatc gcccattttc
    17401 agcgcggaaa catcgacttc aatcggcagc gcgccggagt cttcttgagt gttgaagaaa
    17461 atcggcgcga ttttaccgcc caagcacacg ccgccgaagc gtttgttcgg cacgaacggg
    17521 atgtcttcgc cggtatgcca aatgacggag ttggtcgcgg atttgcgtga agaaccggta
    17581 ccgaccacgt caccgacgta ggcaaccgga tggcctttgg ctttgagttc ttccaacaat
    17641 ttaatcggac cgacttcgcc cggtttgtcg ggcgtgatgc cgtcgcgcgg gtttttcagc
    17701 atggccagcg cgtgcagcgg aatatcggga cgactccacg cgtcgggcgc gggagagagg
    17761 tcgtctgtat tggtttcgcc gtcaactttg aagacggtaa cggtgatttt ttcgggaact
    17821 ttggcacggg aggcgaacca ttcggcatct gcccaagatt gcaaaacttc ctgcgcgtat
    17881 ttgttgcctt tttcggcttt ttcctgaacg tcgtggaagg aatcgaacat cagaagcgta
    17941 tgtttcaagc ctttggcggc aatgggcgcg agtttgtcgt cgtccaagag ttcgattaag
    18001 gcgtggatat tgtaaccgcc gagcatcgta cctaagagtt cggtcgcata tttgggggaa
    18061 accagcgggc tggatgcgct gccttcggca acggcagcca ggaatgaggc tttgactttg
    18121 gcggcatcgt ccacaccggg cggaacacgg tgggcaagca gctcgaccaa gaactcgcct
    18181 tcgcctgcgg gcgggttttt cagcagctca accaaatcgg cggtttgctg cgcattcaaa
    18241 gggagggcgg gaatgccgag ggcggcgcgc tcggcggcgg ctttacgata ggcttctaac
    18301 atctctttgt tcctttttcc gtttttcttt tgtcgggttg caaatgatct gcgttaaata
    18361 ttgtaaacaa catttgtacc gctatcatag actagtttta acaaaaatgg aactgttatg
    18421 acaggtaatg tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgcagacagt
    18481 acaaatagta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc cactataata
    18541 gggaacggct atatgtctgc gggaatgtgt caatgaaaac aaatgccgtc tgaagagagg
    18601 ttcggacggc atttgttcaa cgtttttcct gtttgcgtat cagccaaaca gcgagcaagg
    18661 cgaaggctat ggtaggcagt gcgccggcga ggaagggtgg ggtgccgtag agttggctgg
    18721 taaacccgaa gagcctgccg gcaaggtgga acagcaatcc gagacagatg ccgccgaaga
    18781 gttttaagcc catattgccg tggcgcgtgg tttgcggcgt aaaggcgaag gcaacgagcg
    18841 ccatgaccca tgcggcgacg gggtaaacga gtttacgcca ccatgcgatg gcgtagattt
    18901 gggtgttttg gctgttgttt tggaggtggc ggatgtaggt ggtcagctcg ccgacggaca
    18961 tttggtcggg cttgacgagc aatacgtcca tcaggttgcg tctgacggca atcggccaag
    19021 tttcttcggc ggcggcggat gtttcgattt tgtctgtacc catgatgctg cggcggatgt
    19081 ttttcaactg ccagctgccg tcgctgttca aaacggcgga atcggcttcc actgcctctg
    19141 ccaattcgtt tttatcgttg cgcgcccaaa ttttgatgcc caaaagcgta tggtcgggca
    19201 acattccgcg cacattgata atgctgtttt tttctttcag ccaaaggccg gtatggccgg
    19261 tgctgatttt gccgttaatg gcggcggctt tgatgttttc ggctttttgg ctcagcgtgg
    19321 gcgcaaccca ttcgccgagc gcgacggcgg caatagcaaa aataaaaccg aactgagaca
    19381 gaatcaacag cagctttttg gtgctcatgc cgctggcttt gatgacggcc agttcgctgc
    19441 cggcggcaag ctggctgaga gaggccagtc cgccgatgag gacggcgagg ggcatgagtt
    19501 cgtaggcgcg ggcgggcatt ttgagggcgg tgtagcccag catttcccat atgccgtaac
    19561 tgcctttgcc gaggttgccg gtttcgtaca ggatttcaaa aaagctgtac aaagcgagga
    19621 aggcaaggag cgcgcaaacc gccataaccg ccatttggcg gatgatgtaa cgtgaaatca
    19681 ggttcatttt ccgcctttca atgtcagact tttgccaacc gcctgccaga agggctggct
    19741 gggcatactg cggacgcgca gaagtacgat tgcgatgacg aacatgatga tgtgcatagg
    19801 cagcagtccg agccaaaaat ggattttgcc gtcttccacg gcttcaaaaa gcagggtcag
    19861 cccgttttgg taaattaaaa acaaaccgat ggcaatcaag atattgtagg tatgtccgct
    19921 gcgcgggttg aaataggaaa gcggcacggc gagtaggcag agcaggagga cgctgacggt
    19981 cagcgagatg cgccacatca attctgcctg atgttgcgga ttgctgctgc caatcagttg
    20041 ggcggtcgaa atggtgcggc ggtgggaaac ggggtcgata agtttgggcg tggtgctgat
    20101 aatcaggttg agtttttgga aggaaacctg attgtagtcg gcgcgtccgg gcgtgccgct
    20161 gtaacggtag ccgtggcgca attcgagcgt gcgtttgttg tcgttcagcg agaagttacc
    20221 ttctttggcg aagatgatgt tgtcgccgcc gtttttgtcc tgttcgcgca ggaacaggtt
    20281 tttcatgatg ccggattcgg tgtcaaaggt ttcgacgaaa taaaccctgc cgttgcgctt
    20341 gcccaagtta ttgaactcgc cggcttccac caaagacaat tcctgcttct gcttcaaaat
    20401 ttcggcatat tcgcggctgc gcagttccgc ccacggtatt acccaaagct gcatgacggc
    20461 aatcaggatg gcaaacggca cggcaaactg catgacgggg cgtatccact gtttcaacgc
    20521 caatccgcag gatagccaga ccgacatttc gctgtcgcgc cagtagcggg tcaatacggt
    20581 cagcgtgctg atgaatgcgg tcaacaccag caaaagcggg gtcataccga tgacccagaa
    20641 gccgactaag gccaacacgg catcgatggc gacacgcccg tcagctgcgc ggccaagcag
    20701 gttgatcgcc tgcgtggaca ccaacaccgc caagagggcg acgaaaatgc cgacggcggt
    20761 aaaagagagt tctttgatga ggtttctttg ataaatcata aaatcgcggt caggggggcg
    20821 gtaagcggtt cggcgcaggc ggattgtccg cgcaaggttt cagacggtgc ttcaaaaaat
    20881 ggggtacaat cgtgccagcc gtcttcagac ggtcttttga gcgaaaggcc gtctgaaaac
    20941 taccgacaac cgtctcaatc aggagaataa acgtggaatt tagcacaaaa accgaaatct
    21001 tgcaggaaca gcaggcaggc gcgcagttat ttgtctgtgc cgacaaagca cccgagcaca
    21061 acaccgccgc acatgcgctc ttctctgctt tggaagaggg tcagaatttt tccgacacca
    21121 aaatcccgac ggacaacggt ttgcaggcag tcgccgtcgt ccgcctcgaa aaaaccgacc
    21181 gcgccgcgct gaacaaagcc gccgccgaag ccgccaaatg ggcgcaaaat caagaaacgg
    21241 tcaatgtgga cgttcacgcc tttgatgagg cgcaagcagc agccgttgcc gaagcttttg
    21301 ccatcgcgtt cggcaacgct gcatatcgtt tcgaccgcta caaaaaagaa gccaagcccg
    21361 ccaaattttc gcaagccgtg ttccacagcg cacacgaagc cgccgtcaaa gaagccctgc
    21421 gcgttgccga agcacaagtt tacggacaaa gcctctgccg cgacttaggc aatgccgcac
    21481 cgaacgaatg cacgcctgag ttcctcgcgc gtaccgccaa agccgaagcc gagaaactgg
    21541 gcgcgcacgc caaaatcatc gaaaaagact acatcaaaga aaacatgggt tcgttttggt
    21601 cggttgccaa aggcagcgtc gaagacccat atttggttga actgagctat ttcggtgcgg
    21661 ccgacaaaga agccgcgcct gtggtattgg tcggcaaagg cattaccttc gacaccggcg
    21721 gcatttccct caaacccggc ctgaacatgg acgaaatgaa gtttgacatg tgcggcgcgg
    21781 caaccgtcat cagcaccttc tgcgccgccg tcaaactgca actgccgatc aatctgattg
    21841 ccatcgtcgc cacttgtgaa aacatgcctt ccggtgcagc caacaaaccg ggcgatgttg
    21901 tgaaaagtat gaaagggctg acgattgaag tgttgaacac cgatgccgaa ggccgtctga
    21961 ttttgtgcga cgcgctcact tacgccgagc agttcaaacc caaagccgtc atcgatgtcg
    22021 ccaccctgac cggcgcgtgc atcgtcgcct tggggcacga tgtcagcggc gtgatgggca
    22081 ataatcagga tttgatcgac agcctgcttg ccgcttccta caacgtggac gacaaagcgt
    22141 ggcaactgcc gctctttgaa acctacaaag accagctcaa atccaacttt gccgacatcc
    22201 ccaatatcgg cacgcccggc gcgggcacga ttaccgccgc aacattcctg tcctatttca
    22261 ccgaaggcta cccgtgggcg caccttgaca tcgcgggtac ggcgtggaaa tccggagcgg
    22321 aaaaaggtgc gaccggccgc cccgtaccgc tgctgatgaa ctatctgcgg aatctttaat
    22381 tttgccacaa tgccgtctga agcttttcag acggcatcat cctgtcaaat gctcaaacaa
    22441 tatgccgaaa gttacctttt atacccatgt tgaccaaatc ccccttttta cctgtcggtt
    22501 gattgcgcgc gctatccgag acggcggcag gatactggtg tggtccgact cgttcgggcg
    22561 gcttcaggaa ttggacaaaa tgctttggca atacgaggcc gagagtttta ttccgcacga
    22621 aatttgggaa acggaagaag ccatgccgtc tgacacatcc gtcctgcttg cctgcggcgg
    22681 caatctgccc cgaattcccg aaggcatggc cgttttgaac ctgtccgacg gtttttggaa
    22741 caccgcttcg gtcttgcccg cgcgcgtttt ggaaatcgtc ggcaacagcc tggaagatct
    22801 cgccgacgcg cgcgaacgct ttaccgccta ccgccgaagc ggttttgcca tcgaacatca
    22861 cggcatggag ggcaaggcat gaaatgccgt ctgaaatgcc gcatttacaa aatttaaaca
    22921 ggcttgattc tatttgatta aaatttcctt atcggttgaa ccccgccact tggacatctg
    22981 tccttcgggg cggtagaatc aaaccttatt ttggaaggtt caatcccttc caaaacaggg
    23041 caacacacag attgacgctt tatgtgccat cctgtgtgtt gaaacattca aactcggcta
    23101 taatcccgtt tttccgactt tatcgacagc gaagatccat catgaacacc attttcaaaa
    23161 tcagcgcact gaccctttcc gccgctttgg cgctttccgc ctgcggcaaa aaagaagccg
    23221 cccccgcatc tgcatccgaa cctgccgccg cttctgccgc gcagggcgac acctcttcaa
    23281 tcggcagcac gatgcagcag gcaagctatg cgatgggcgt ggacatcgga cgctccctga
    23341 aacaaatgaa ggaacagggc gcggaaatcg atttgaaagt ctttaccgat gccatgcagg
    23401 cagtgtatga cggcaaagaa atcaaaatga ccgaagagca ggcccaggaa gtgatgatga
    23461 aattcctgca ggagcagcag gctaaagccg tagaaaaaca caaggcggat gcgaaggcca
    23521 acaaagaaaa aggcgaagcc ttcctgaagg aaaatgccgc caaagacggc gtgaagacca
    23581 ctgcttccgg tctgcagtac aaaatcacca aacagggtga aggcaaacag ccgacaaaag
    23641 acgacatcgt taccgtggaa tacgaaggcc gcctgattga cggtaccgta ttcgacagca
    23701 gcaaagccaa cggcggcccg gccaccttcc ctttgagcca agtgattccg ggttggaccg
    23761 aaggcgtacg gcttctgaaa gaaggcggcg aagccacgtt ctacatcccg tccaaccttg
    23821 cctaccgcga acagggtgcg ggcgaaaaaa tcggtccgaa cgccactttg gtatttgacg
    23881 tgaaactggt caaaatcggc gcacccgaaa acgcgcccgc caagcagccg gatcaagtcg
    23941 acatcaaaaa agtaaattaa gtccgaatcc atgcccgaaa caggttttcg ggcattttta
    24001 cggcaaatgc cgtctgaagc caccaaacag cggttcagac ggcatccctt tcaaaagcgc
    24061 accattatga aaaacatcgt catcctgatt tccggacgcg gcagcaatat gcaggcaatc
    24121 gtcaatgccg ccattcccaa cgtccgcatt gccgccgtgt tgagcaacag cgaaacggct
    24181 gccggtttgc aatgggcggc cgaacgcggc atcccgaccg acagcctgaa tcataaaaac
    24241 tttgaatcac gacttgcctt cgatacggcc atgatggaaa aaatcgacgc atatcaaccc
    24301 gatttggtgg ttttggcagg ttttatgcgg attctgaccc ctgagttctg cgcccattac
    24361 gaaggcaggc tgatgaacat ccatccgtcc atcctgccct cgttcaccgg gcttcatacg
    24421 cacgaacgcg ctttggaagc aggttgccgc gttgccggct gtacgattca tttcgttacg
    24481 gccgagctgg attgcggccc gattgtatcg caagggattg tgccgatact tgacggcgat
    24541 acggcagacg atgttgccgc ccgggttttg gctgtcgaac acaaactcta tccgaaagcc
    24601 gttgccgatg ttgccgccgg ccgcctgatt atcgagggaa accgtgtcag aaattcggaa
    24661 aacgccgatg ccgcccgttt cctgacggcg taaaccgggc gggagcaaat gatgaagact
    24721 tttaaaaata tattttccgc cgccattttg tccgccaccc tgccgtgcgc gtatgcggca
    24781 aggctacccc aatccgccgt gctgcactat tccggcagct acggcattcc cgccacgatg
    24841 acatttgaac gcagcggcaa tgcttacaaa atcgtttcga cgattaaagt gccgctatac
    24901 aatatccgtt tcgaatccgg cggtacggtt gtcggcaata ccctgcaccc tgcctactat
    24961 aaagacatac gcaggggcaa actgtatgcg gaagccaaat tcgccgacgg cagcgtaacc
    25021 tacggcaaag cgggcgagag caaaaccgag caaagcccca aggctatgga tttgttcacg
    25081 cttgcctggc agttggcggc aaatgacgcg aaactccccc cgggtctgaa aatcaccaac
    25141 ggcaaaaaac tttattccgt cggcggcctg aataaggcgg gtacgggaaa atacagcata
    25201 ggcggcgtgg aaaccgaagt cgtcaaatat cgggtgcggc gcggcgacga tacggtaacg
    25261 tatttcttcg caccgtccct gaacaatatt ccggcacaaa tcggctatac cgacgacggc
    25321 aaaacctata cgctgaagct caaatcggtg cagatcaacg gacaggccgc caaaccgtaa
    25381 atcggttttc aggcggtttt ttgccgtttt gtccatattt ctgtataagc cgcttgaaat
    25441 tgtttacaat aacgccatat gcaaagcggt tttaacgcta ttttcgggaa tgacaccatg
    25501 caggttacat caaaatggat agacggaatg tgttttgtcg gcacggcgga aggcgggcac
    25561 agcgtcgtta tggagggatc ggcggcagaa ggcgcggcca agcgcgggcc cagccctttg
    25621 gaaatgctgc tgttgggggt ggcgggctgt tcgagcatcg atgtggtgat gattgccgaa
    25681 aaacagcgtc agaaagtgac tgactgccgt gcgacggtta cggcgaaacg ggcggacgat
    25741 gcgccgcgcg tgtttaccga aatccacatc catttcaagg taatcgggca tgatttgaaa
    25801 gaatcggcca ttgagcgcgc cgttcagatg tctgccgaaa aatactgttc ggcttcgatt
    25861 atgttgggca aagcggcaaa gattacccac agttttgaaa ttgccggggc ggagaaatag
    25921 gcatcgggcg gcattatggg gaaacggctg atgccgtctg aaggggaaag gtgcggcagg
    25981 caatccggag gcgcataaaa aagcagatat attcggactg cacctcccga atatatctgc
    26041 ctgctgtttc ctctttattc agcctttata atacttggac ttgtcggggt attgtgcagg
    26101 cttgattccg gattgtcaac aattttcggt caaattttaa atgccgcgtt ttaaaatgat
    26161 gcccgcctga ttttgcgggc gggcggaagg cgggaatccc gttattttaa gccatatatt
    26221 aatttattga attaaaataa atttgtgcaa tatagtggat taacaaaaac cagtacggca
    26281 ttacctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tactgattta
    26341 aatttaatcc actatacttt aatttctcct acacgatttg aaacacagcc gtcttaaaac
    26401 atcttaaaaa atttgaaagc gtaaaaatac ggaaaacatc atgaactttg aaaacgacga
    26461 cattatccat gcgccgacca cgtcttccct gattctcgaa gagcggcacg attcggagct
    26521 gttccgtgtt tacgccctga ttttggacgg cattaccgat caggtgctgc tgcccggcaa
    26581 aaagctgacc gaatccgaac tttgccgtca gatggtgtgt tcgcgcaaca ccgtccgcgg
    26641 cgcgctgtcg cttttggcgc acgacaagat tgtcgatttg caacccaaca ggggcgcgtt
    26701 cgtccacgtt cccgatttga aagaaatgca ggatgtgttc aatgcgcgca tcgaaatgga
    26761 gacgatgatt ttgaacatcc tcgccggcct gccggatttg gaaacgcgcc tcaagccgct
    26821 ttatgcgatg ataaggtgcg aagaagaggc ctccggcagg ggcgaccgcg tcggctggaa
    26881 ccgcctgtcc aatgccttcc acgtcgaact ggcgcgcctg gtgggcaacg atgtgctgtt
    26941 cgacattatg aacacgctgt gcgcgcggtc ttccctgatt gtcgccgtgg cgggcgtgca
    27001 tcgcgagaaa aaacacgcca tcaatacgca tacgcattcc gaacaccgcg aaatcctcga
    27061 cctgctgctg gcgggcaggc gcaaccgcgt ggtcaaaatc ctgcgccgcc atttgggcag
    27121 ctgtatggag cgtttggaaa agactttgga agattgaatg ccggagcgga taagccgcct
    27181 gaaccttcag gcggtttttt aatggcgaac ctgatgccgt ctgaaatatg gatgcgggta
    27241 tctgcaattt tcagacggca atttttaagc cgcacatatc atgcggcaat aaaggagggt
    27301 aggggatgag cagcctgatg acgttgtttt cggtattggt accgatgttt gccggatttt
    27361 ttatccgtgt tcccaagcct tacctgcccg cttcggacaa ggtgctgtcg gttttggtgt
    27421 atgccgtgct gctgctgatc ggcgtatcgt tgtcgcgcgt ggaggatttg ggttcgcggt
    27481 tgggcgatat ggcgttgacg gttctgtggc tgtttgtttg tacggtaggg gcgaacctgc
    27541 ttgccttggc agtgttggga aagttgtccc cgtggcggat agggggaaaa gggaagggcg
    27601 tttcggtcgg cgtgtcgggc agtgtgaggc agctcggatg cgtactgatc ggttttgtgt
    27661 ccggcaaatt gatgtgcgat atttggatgc cgtctgaaaa cgcgggtatg tactgcctga
    27721 tgctgctggt gttcctcatc ggcgtacagc tcaaaagtag cggcgtatcg ttgcggcagg
    27781 ttttgcttaa ccggcggggc atccggctgt cggtttggtt tatattgtca tctctttcag
    27841 gcgggctgct gtttgccgca tcggcagatg gtgtgtcgtg gacgaaaggt ttggcgatgg
    27901 cttccggctt cggttggtat tccctctcgg gtttggtaat gaccgaggct tacggggcgg
    27961 tatggggcag catcatgctg ctgaacgatt tggcacgaga gctgtttgca ctggcattta
    28021 ttccgctgct gatgaagcgt tttccggatg cggcggtggg ggtcggcggc gcgaccagta
    28081 tggatttcac attgcccgta attcagggtg cgggcggttt ggaagtcgtg ccggtagcgg
    28141 tcagcttcgg cgtggtggtc aatatcgccg ccccgtttct gatggtggtg ttttccacgc
    28201 tgggctgaac gcggtaaaat cggcatcccg atgcaaggaa gcaaaaaacg atgaaaccga
    28261 aaatccaaag gcatggagag attttaagcc ttgtccgccg gcatcagttt atgtcggtgg
    28321 acgagcttgc cgccgcattg gacgttaccc cgcagacgat acgttgcgac atccgcgagt
    28381 tggaggaggg cggcagcctg aaacgccatc acggcggcgc atcttcgggc ggaaacttgc
    28441 cggagggtct gcccgccgac cgccaaaccc ggtgtcaaaa cgaaaaaaac gccattgccc
    28501 ggctgattgc ggaacacatc cccgacggtt cgtcgctgtt cgtcagtatc ggtacgacca
    28561 tggaagccgc ggcatcagag ctggtgaagc ggcgcagcag cctgcggatt attaccaaca
    28621 atatccacgt cgcctccgtc gtttcggcac gtacggatta cacggtcatc atcacatccg
    28681 gcgtcgtccg ccctttggac ggcggtatta ccggcgtggc gaccgtcgac tttatcaacc
    28741 agttcaaagt cgattatgcc gtgatgagca cgcacggcgt ggaaagcgac ggttcgcttt
    28801 tggattacga ttacaaggaa gtcagcgtca tgcaggcgat gattgccaac gcgcgcgtcc
    28861 gttttctcgg cgtggatcac agcaaattcc gcagcaacgc gctggtcagg ctcggcggca
    28921 ttacggcgtt tgacaaagta tttaccgaca ggttgcctga taccgcgatg cagaagatgc
    28981 tgaaagaggc gggggtggaa tgcctgattg ccgatgccgt ctgaacgcta tgtcaaagcg
    29041 cgcaagtcgg gtacaataaa cacatcatca aaccgcttca gacggcatac ggaacctccc
    29101 caatgccgtc tgaagccatc tgtttaaaga aaaccatgct caataaagac caattcgcgg
    29161 acaaccattt catccgcacc atcatcgaag acgacctcaa aagcggcaaa cacgaagccg
    29221 tccaaacccg ttttccgccc gaacccaacg gctatttaca tatcggacac gccaaatcca
    29281 tctgcctgaa cttcggtttg gcgtatattt acgacggttt gtgcaacctg cgtttcgacg
    29341 acaccaaccc ggaaaaagaa aacgacgaat acgtcaacgc catcaaagaa gacgtcgagt
    29401 ggctcggttt ccattgggcg ggcgaaccgc gtttcgcttc cgactatttc gaccggcttt
    29461 atgactacgc cgtcggttta atcaaagacg gaaaagcgta tgtcgatgat ttgacgcccg
    29521 aagaaatgcg agaataccgc ggtacgctga ccgaagcggg taaaaacagc ccttaccgcg
    29581 accgcagtat cgaagaaaac ctcgacctgt tcacgcgtat gaaaaacggc gagttccccg
    29641 acggcagcaa aaccctgcgc ctgaaaatcg acatggcggc aggcaacatc aatatgcgcg
    29701 atcccgtcat ctaccgcatc cgccgcgccc atcaccacaa caccggcgac aaatggtgca
    29761 tctacccgat gtacgattac acgcattgca tttccgatgc catcgaaggc atcacgcatt
    29821 ccttgtgcac gctcgaattt gaagcgcacc gtccgcttta cgactgggtg ttggacaaca
    29881 tccccgcgct gcacgccacc cgtccgcgcc aatacgagtt ttcccgtttg gagcttttgt
    29941 acaccattac ctccaaacgg aaattgaatc agttggttgt ggaaaaacac gtttccggct
    30001 gggatgatcc gcgtatgccg accatttccg gtatgcgccg ccgcggctac acgcccgaag
    30061 gggtgcgcct gtttgccaaa cgcgccggta tttccaaatc tgaaaacatc gtcgacatga
    30121 gcgtgttgga gggtgcgatt cgcgaagagc tggaaaactc cgccccgcgc ctgatggcgg
    30181 ttttgaaccc gctcaaagtg accctgacca actttcaagc cggcaaaacc caaagccgcc
    30241 gtgccgcgtt ccatccgaac cacgaagaaa tgggcgatcg cgaagtacct gtttcacaaa
    30301 ccatctacat cgaagccgac gactttgccg aaaatccgcc caaaggcttc aagcgtctga
    30361 ttcccggcgg cgaagtacgc ttgcgccacg gctatgtcat caagtgcggt gaagtcgtca
    30421 aagacgaagc aggcaatgtg gttgaactca aatgcagcat cgaccacgac accttgggca
    30481 aaaatccaga aggccgcaaa gttaaaggcg tgattcactg ggtttccgcc gaacacgccg
    30541 ccgaaatcaa agtccgtctg tacgaccgcc tctttaccgt cgagcgtccc gatgccgtgc
    30601 gcggcgaaga cggcgaatac ctgccgttta ccgatttcct caatccggaa tccgttaagg
    30661 aaatcactgc ttacgccgaa cctgccgcga aagatttgcc ggcggaaagc cgttggcagt
    30721 tcgagcgtat cggctatttt gtgaccgacc gccaagacca cggcaaagac acgccggtgt
    30781 ttaaccgcac ggtgacgttg aaagattctt ggcagcctaa gtaaacccca tccttgccgt
    30841 ctgaatattg ttcgggcggc atttctcctt tacccgcgaa atgcggcaca ttcggcacac
    30901 cggaaaggaa atatgatgaa agtcctcttt atcgccgacc cgatggcaag tttcaaaacc
    30961 tacaaagaca ccacctacgc gatgatgcgg gaaatggcaa aacgcggctg gcggctgttt
    31021 cataccttga gcggggaatt gtctgtaaac ggcggtttgg taacggcaca ggcatcggca
    31081 tttgaatttt cgggtgcaaa aaacgatgat gaccatgaat ggtttaaagc ggcggacaaa
    31141 gttcagacgg cattaaaaga atttgatgcc gtgattatgc gtaccgatcc gccgttcgat
    31201 atgcaatacc tttactccac ccaattactg acgctggcgg aacagcaggg cgcgaaagtg
    31261 ttcaacagcg gacgggcgat gcgcgacttt aacgaaaaac tggcgatttt gaatttcagc
    31321 cgctttaccg cgcccacgct ggtaacgacc cgttccgccg atgtccgcgc atttttgaaa
    31381 gaacacggcg acatcatcgt caaaccgctc gacggcatgg gcggcatggg catcttccgc
    31441 ctgaccgaaa aagaccccaa catcggcagc atcctcgaaa ccctgatgcg gtttgattcc
    31501 cgcaccatta tggcgcaacg ctacattccc gaaatcgtac acggtgacaa acgcatcttg
    31561 attatcggcg gcgaagtcgt cccctatgct ttggcgcgta tcccgcaaaa cggcgaaaca
    31621 cgcggcaatc tggcggcagg cgggcgcggt gtggcgcagg aattggacgg acgcgaccgg
    31681 gaaattgcag agactctggc tcccgagctt aaacggagcg gcatcctgct ggccggtttg
    31741 gacgttatcg gcagcaacct gaccgaagtc aacgtaacca gcccgaccgg attccaagaa
    31801 attatgaaac aaaaaagttt cgacgtggcg gcaatgtttg ccgatgccgt tgccgcgtgg
    31861 tcggtacgtt aaaccgatgc cgtctgaaag gcttttgctt cgtaaccgcc tggtttgttc
    31921 gggcaggcag ggttttccct cagccggcag ggatgcgctt aatgccgtac ggacacccct
    31981 ccgccccgtt ttcagacggc atatattgag gacattttga aaggataccg atggaacctt
    32041 cctcctacgc ggcagaaaaa aaaggcaaaa gcggcatcag gcgcgtcatc aacgcattcg
    32101 gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc cgtcaggttt
    32161 tgtggctgaa cgcgctgctg gtgtgcgcgg catttttttg ggtttccgaa accgccgtcc
    32221 gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc aacaccgccg
    32281 tcgaagccgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa cgcgccaaag
    32341 atgcaggttc tgccgcacaa ctggttgcca tgctgatgtt ggcggcggtt tggctgtccg
    32401 ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta gtatttatta
    32461 ttcacttaat ctacatcaaa tttccgagca gtatttgtat gtaagattaa gcacattccc
    32521 cgtctgatat taaaggagca ggaagatgaa aaaattattg gcagccgtga tgatggcagg
    32581 tttggcaggc gcggtttccg ccgccggagt ccatgtcgag gacggctggg cgcgcaccac
    32641 tgtcgaaggt atgaaaatgg gcggcgcgtt catgaaaatc cacaacgacg aagccaaaca
    32701 agactttttg ctcggcggaa gcagccccgt tgccgaccgc gtcgaagtgc atacccacat
    32761 caacgacaac ggcgtgatgc gtatgcgcga agtcaaaggc ggcgtgcctt tggaggcgaa
    32821 atccgttacc gaactcaaac ccggcagcta tcacgtgatg tttatgggtt tgaaaaaaca
    32881 actgaaagag ggcgacaaga ttcccgttac cctgaaattt aaaaacgcca aagcgcaaac
    32941 cgtccaactg gaagtcaaaa ccgcgccgat gtcggcaatg aaccacggtc atcaccacgg
    33001 cgaagcgcat cagcactaat ctgctgaaaa tatttgaaat gccgtctgaa aaagcccggg
    33061 ctttcagacg gcatttttat gcccgccttt aaaatgtgtt aaaatccgcc tttaaaaacc
    33121 gccgtttcca agccatcctg cgtatgaata cgacatcaaa cacttccaat atcatcgtcg
    33181 ggctttccgg cggtgtcgat tcttccgtaa ccgccgccct gctcaagcag cagggttatc
    33241 aagtgcgcgg tgtgttcatg cagaactggg aaaacgacga caacgacgaa tattgcagca
    33301 tcaaacagga ttcgttcgat gccatcgccg ttgccgatat tgtcggcatc gacatcgaca
    33361 tcgttaattt cgccgcgcaa tataaagaca aagtttttgc ttattttctt caggaataca
    33421 gtgcggggcg cacgccgaat ccggatgtgt tgtgcaatgc cgaaatcaaa ttcaaatgct
    33481 ttttggacta cgccgtaggg cagggcgcgg ataccattgc caccggacac tatgcgcgca
    33541 aagaagtccg caacggcgtg cattacctgc tcaaaggttt ggatcgaaac aaagaccaaa
    33601 gctattttct ctaccgcctc aagcctttcc aactcgaacg cgcgattttt ccgttgggcg
    33661 gtttggaaaa acccgaagtg cgccgccttg ccgccgaatt taatttgccg actgccgcta
    33721 aaaaagacag taccggcatc tgtttcatcg gcgagcgtcc gttccgcgag tttctgcaga
    33781 aatacttgcc gaccgacaac ggcaaaatgg tgacgcccga agggaaaacc atcggcgaac
    33841 acgtcgggct gatgttttac acattgggtc agcgcaaagg attgggcatc ggcggcgcgg
    33901 gcgaaccgtg gtttgttgcg gctaaagatt tgacgaaaaa cgaactcatc gtcgtacaag
    33961 gacacgacca tccgctgctc tatacccgca gccttgtgat gaacgatttg agtttcacgc
    34021 tgcccgaacg tccgaaggca ggacgctata cctgcaaaac gcgttaccgt atggcggacg
    34081 cgccttgcga attgtgctat ttggatgatg aaaccgccga gctggtgttt gacgaaccgc
    34141 aatgggcggt tacgccgggt cagtccgccg tgctgtacga cgtcgacatc tgtttgggcg
    34201 gcggcatcat ccaaacgacc gacaaacccg tcatcatcac gcgataaagg taatgccgtc
    34261 tgaaacggtt ttcagacggc attgttccgc tcaattccac tttaaagacc gatacctatg
    34321 gaaaaaatct ggttagaaag ctacgagaag ggcgtcagtg ccgaaatcga catcacgcaa
    34381 tacaattccg tcagcgacgt attccgccaa agcgtggaaa aatttgcccg tctgcccgct
    34441 tttcaaaata tgggcaaaac gctcacttat gccgaaaccg gcaaactggc gaccgatttc
    34501 gcctcttatc tgcaaaacgt cctcaagctg ccgcgcggcg aacgtgttgc cattatgatg
    34561 ccgaacgtat tgcaatatcc gattgccctt ttcggtattt tgcaggcagg tttggtggcg
    34621 gtgaacacca atccgctcta tacgccgcgc gagttggagc atcagctgaa agacagcggt
    34681 gcgaccgcca tcatcgtttt ggaaaatttc gctaacacgc tggagctggt gctgccgcgc
    34741 acgcagatca aacacgtcat cgtcgcctcc gtcggcgaaa tgttcgggct gcttaaaggt
    34801 tcgctgatca atttcatcat ccgaaaaatc aagaaaatgg ttcccgaata ccgtattcgg
    34861 gaaaccgttt cctttcagac ggcattgaaa gagggggcga agcacgtttt ccaacctgtc
    34921 gcattaaacc gcgaagatac cgcattgttg caatacacgg gcggcacgac aggcgttgcc
    34981 agaggcgcgg tgctgagcca cggcaacatc tgcgccaata tgcttcaggc aaaagaatgg
    35041 attaaaaacc aattgcgcga gggaaaagaa accgttatcg ccgccctgcc gctgtaccac
    35101 atcttcgccc tgacagtgaa tctgatgatt tttgccaatg ccggctcgaa aattgtcctg
    35161 attgccaacc cgcgcgatat gaaaggcttt atcggcgaac tgaaaaagca gcgggttaac
    35221 gtatttatcg gcgtgaacac gctgtttaac gcgatggtca accggcccga tttcgccgaa
    35281 gtcgattttt caggattgcg gctgactttg ggcggcggta tggcgaccca aaaagccgtt
    35341 gccgaaaaat ggaaaaaaat caccggcacg cccatcgtcg aagcctacgg tttgaccgaa
    35401 gccagccccg gcgtgtgctg caacccctta aacatcgaat catacagcgg cagcatcggt
    35461 ttgcccgtcc cgtccaccga agtcgaactg cgcgacgcaa acggcaaaga agtccccgtt
    35521 gggcagccgg gcgaattgtg ggtaaaaggc ccgcaagtga tgcaaggcta ctggaaccgc
    35581 cccgaagaaa ccgccaaagc catagacgcg tgcggctttt tggaaaccgg cgatattgcc
    35641 gtgatggacg aaaaaggccg tctgaagctg gtcgatcgca aaaaagacct cgtcgtcgtt
    35701 tccggattca atgtttatcc gaacgaaatc gaggaattca tcgcgcacca cgaaaaagtt
    35761 atggaagttg cgtgtatcgg cgtacccgac gaaaaaaccg gcgaggcact caaagtgttc
    35821 gtcgtcaaaa aagacccgtc tttgacccaa gaagaactca ccgctttctg ccgtaccggt
    35881 ctgaccgcat acaaagtgcc gaaagacatc gaattccgcg acgagttgcc caagtccaat
    35941 gtcggcaaaa tcctgcgccg cgagttgcgc caaagtgccg ggaaataaag aaaagatacc
    36001 gtctgaaaac ggtcgtccac cgttcagacg gcatccgtcc gtttgcaaga accgcgcgct
    36061 tcacgttaaa atcacgcatt ccaacatggg tattccatca tgaccaaatt cattttcgta
    36121 accggcggcg ttgtctcctc actgggtaaa ggtatcgccg ccgcttctat tgccgccatc
    36181 ctcgaatcgc gcggcttgaa cgttaccatg ctcaagcttg atccttatat caacgtcgat
    36241 cccggcacga tgagcccgtt ccaacacggc gaagtgttcg taaccgacga cggcgcggaa
    36301 accgacctcg acttgggaca ctacgaacgc ttcatcgatt ccacgatgac ccgccgcaac
    36361 agcttcagca cgggtcaggt gtacgaaaac gtcatcgcca aagaacgccg cggcgactat
    36421 ctcggcggta cggttcaagt catcccgcac attaccgacg aaatcaaacg acgcatccat
    36481 gaaggcgcgg cgggttacga tgtggcgatt gtcgaaatcg gcggtacggt cggcgacatc
    36541 gaatcgctgc cgtttttgga agccatccgc cagatgcgaa gccagttggg acgcaacaac
    36601 accctgttcg cccacttgag ctacgttccc tacatcgccg ccgcaggcga aatcaaaacc
    36661 aagccgaccc agcacaccgt gaaagaaatg ttgagcatcg gcttgcaacc cgacatcctg
    36721 atttgccgta tggacaggaa aatgccggca gacgaacgcc gcaaaatcgc cttgttctgc
    36781 aacgtggaag agcgcgcgat tgtcggcagc tacgatgtgg acagcatcta cgaatgcccc
    36841 gaaatgctgc acgaccaagg catcgacaac atcattaccg agcagttgca gcttaacgtg
    36901 cagcaggcgg atttgaccgc gtggaaaaaa atcgtccacg ccgtcaaaaa cccgaaacac
    36961 accgtcaaaa tcgcgatggt cggcaaatac gttgatttga ccgaatccta caaatcattg
    37021 attgaagcct tgaaacacgc gggcatccat accgaaaccg atgtgcagat taccttcgtt
    37081 gacagcgaaa gcatcgagaa aaacaagggc gacgtttccg tactcaaaga tatggatgcc
    37141 atcctcgttc ccggtggctt cggttcgcgc ggcgtggaag gcaaaatcgc cgccgtgcgc
    37201 tacgcccgtg aaaacaacgt gccatacttg ggcatctgcc tcggtatgca gattgcgctg
    37261 attgaatacg cccgcgacgt ggcaggtttg aaaggtgcga attccactga gtttgacttg
    37321 aaatgcgctg cccccgtcgt cgccctgatt gacgaatggc aaaccgccga cggcagcgtc
    37381 gaaacccgtg acgaatccgc cgatttgggc ggcacgatgc gtttgggcgc gcaagaagtc
    37441 gaattgaaag caggcagcct cgccgtcaaa atctacggca gcggacacat tcgcgaacgc
    37501 caccgccacc gctacgaagt caacaacaac tacgtttccg cgctggaaca ggcaggtttg
    37561 gtcatcggcg gcgtatccgc cggacgcgaa cgcttggtcg aaaccatcga actgccgaac
    37621 catccttggt tcttcgcctg ccagttccat cccgagttca cgtccaaccc gcgcaaaggg
    37681 catcctttgt tcaccgcgtt tgtcaaagcc gcgttgaaca ataaaaaagc ctgataaagc
    37741 gttacttgat gatcaaaatg ccgtctgaaa gcctgacaaa gactttcaga cggcattttc
    37801 gcaaatcggg gatcataaaa gccatcaatt taatttgggt ctgtac
//
LOCUS       DAXFHF010000014        37758 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXFHF010000014 DAXFHF010000000
VERSION     DAXFHF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37758
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.014"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            42..2219
                     /gene="tdfF"
                     /locus_tag="ACFIRM_000600"
     CDS             42..2219
                     /gene="tdfF"
                     /locus_tag="ACFIRM_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC3028288.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPFGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     PDGTGEPSATVNLVRKHPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQLERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWSNSRNRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHSTAATDLIPGYWHADPRTHSASMSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYDTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRAGSYNSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQLQ
                     KDEHGSYLKPVTGNNLEADIKGEWLEGRLNASAAVYRARKNNLATAAGRDQSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKPRDQDGSRLNPDSVPERSFKLFTAYH
                     LAPEAPSGRTIGAGVRRQGETHTDPAALRIPNPAAKARAVANSRQKAYAVADIMARYR
                     FNPRTELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
     gene            complement(2480..2803)
                     /locus_tag="ACFIRM_000601"
     CDS             complement(2480..2803)
                     /locus_tag="ACFIRM_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3028289.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(2884..3540)
                     /locus_tag="ACFIRM_000602"
     CDS             complement(2884..3540)
                     /locus_tag="ACFIRM_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC3028290.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(3643..4170)
                     /locus_tag="ACFIRM_000603"
     CDS             complement(3643..4170)
                     /locus_tag="ACFIRM_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC3028291.1"
                     /translation="MYANMGDMMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWLWVGFYFVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGEL
                     AKILERRFEASRQAA"
     gene            4329..5102
                     /gene="tpiA"
                     /locus_tag="ACFIRM_000604"
     CDS             4329..5102
                     /gene="tpiA"
                     /locus_tag="ACFIRM_000604"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC3028292.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5109..5459
                     /gene="secG"
                     /locus_tag="ACFIRM_000605"
     CDS             5109..5459
                     /gene="secG"
                     /locus_tag="ACFIRM_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC3028293.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            5473..5558
                     /locus_tag="ACFIRM_000606"
     tRNA            5473..5558
                     /locus_tag="ACFIRM_000606"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5507..5509,aa:Leu,seq:gag)
     gene            5941..6062
                     /locus_tag="ACFIRM_000607"
                     /pseudo
     CDS             5941..6062
                     /locus_tag="ACFIRM_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6448..6815
                     /locus_tag="ACFIRM_000608"
                     /pseudo
     CDS             6448..6815
                     /locus_tag="ACFIRM_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6812..7123
                     /locus_tag="ACFIRM_000609"
     CDS             6812..7123
                     /locus_tag="ACFIRM_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3028294.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPGGAALKLLTIVKNKGIEAI
                     A"
     gene            7129..7389
                     /locus_tag="ACFIRM_000610"
     CDS             7129..7389
                     /locus_tag="ACFIRM_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028295.1"
                     /translation="MANGKISKFTYRRAAEYACQDRQDRRTEYQGVAAQMPDRIKPSR
                     NGGGTDGIARQQGTAQTGAKPETSPTARHGDFLRPLQGGDWI"
     gene            complement(7897..8452)
                     /locus_tag="ACFIRM_000611"
                     /pseudo
     CDS             complement(7897..8452)
                     /locus_tag="ACFIRM_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8449..9256)
                     /locus_tag="ACFIRM_000612"
                     /pseudo
     CDS             complement(8449..9256)
                     /locus_tag="ACFIRM_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(9249..9419)
                     /locus_tag="ACFIRM_000613"
                     /pseudo
     CDS             complement(9249..9419)
                     /locus_tag="ACFIRM_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(9386..10177)
                     /locus_tag="ACFIRM_000614"
     CDS             complement(9386..10177)
                     /locus_tag="ACFIRM_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC3028296.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(10406..13036)
                     /gene="leuS"
                     /locus_tag="ACFIRM_000615"
     CDS             complement(10406..13036)
                     /gene="leuS"
                     /locus_tag="ACFIRM_000615"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC3028297.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGNKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            13303..13819
                     /locus_tag="ACFIRM_000616"
                     /pseudo
     CDS             13303..13819
                     /locus_tag="ACFIRM_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            13826..14269
                     /locus_tag="ACFIRM_000617"
     CDS             13826..14269
                     /locus_tag="ACFIRM_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC3028298.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            14438..16495
                     /gene="ppk1"
                     /locus_tag="ACFIRM_000618"
     CDS             14438..16495
                     /gene="ppk1"
                     /locus_tag="ACFIRM_000618"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC3028299.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALIKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYQASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(16644..17747)
                     /gene="dnaN"
                     /locus_tag="ACFIRM_000619"
     CDS             complement(16644..17747)
                     /gene="dnaN"
                     /locus_tag="ACFIRM_000619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC3028300.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(17827..19383)
                     /gene="dnaA"
                     /locus_tag="ACFIRM_000620"
     CDS             complement(17827..19383)
                     /gene="dnaA"
                     /locus_tag="ACFIRM_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC3028301.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            19675..19809
                     /gene="rpmH"
                     /locus_tag="ACFIRM_000621"
     CDS             19675..19809
                     /gene="rpmH"
                     /locus_tag="ACFIRM_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC3028302.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            19812..20177
                     /gene="rnpA"
                     /locus_tag="ACFIRM_000622"
     CDS             19812..20177
                     /gene="rnpA"
                     /locus_tag="ACFIRM_000622"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC3028303.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            20242..20463
                     /gene="yidD"
                     /locus_tag="ACFIRM_000623"
     CDS             20242..20463
                     /gene="yidD"
                     /locus_tag="ACFIRM_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC3028304.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            20636..22273
                     /gene="yidC"
                     /locus_tag="ACFIRM_000624"
     CDS             20636..22273
                     /gene="yidC"
                     /locus_tag="ACFIRM_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC3028305.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSAQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(22354..23064)
                     /locus_tag="ACFIRM_000625"
     CDS             complement(22354..23064)
                     /locus_tag="ACFIRM_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3028306.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(23126..23716)
                     /locus_tag="ACFIRM_000626"
     CDS             complement(23126..23716)
                     /locus_tag="ACFIRM_000626"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC3028307.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            23864..24364
                     /locus_tag="ACFIRM_000627"
     CDS             23864..24364
                     /locus_tag="ACFIRM_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC3028308.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADKELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            24398..24577
                     /gene="rpmF"
                     /locus_tag="ACFIRM_000628"
     CDS             24398..24577
                     /gene="rpmF"
                     /locus_tag="ACFIRM_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC3028309.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            24752..25279
                     /locus_tag="ACFIRM_000629"
     CDS             24752..25279
                     /locus_tag="ACFIRM_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC3028310.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            25369..26415
                     /gene="plsX"
                     /locus_tag="ACFIRM_000630"
     CDS             25369..26415
                     /gene="plsX"
                     /locus_tag="ACFIRM_000630"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC3028311.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            26553..26804
                     /locus_tag="ACFIRM_000631"
     CDS             26553..26804
                     /locus_tag="ACFIRM_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC3028312.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            26831..27049
                     /locus_tag="ACFIRM_000632"
                     /pseudo
     CDS             26831..27049
                     /locus_tag="ACFIRM_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27200..27391
                     /locus_tag="ACFIRM_000633"
                     /pseudo
     CDS             27200..27391
                     /locus_tag="ACFIRM_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27421..27624
                     /locus_tag="ACFIRM_000634"
     CDS             27421..27624
                     /locus_tag="ACFIRM_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028313.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            27651..28613
                     /locus_tag="ACFIRM_000635"
     CDS             27651..28613
                     /locus_tag="ACFIRM_000635"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC3028314.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            28660..29031
                     /locus_tag="ACFIRM_000636"
     CDS             28660..29031
                     /locus_tag="ACFIRM_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC3028315.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            29057..29983
                     /gene="fabD"
                     /locus_tag="ACFIRM_000637"
     CDS             29057..29983
                     /gene="fabD"
                     /locus_tag="ACFIRM_000637"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC3028316.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHVIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAETLKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            30122..31990
                     /gene="msbA"
                     /locus_tag="ACFIRM_000638"
     CDS             30122..31990
                     /gene="msbA"
                     /locus_tag="ACFIRM_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC3028317.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPAELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            32090..33655
                     /gene="guaA"
                     /locus_tag="ACFIRM_000639"
     CDS             32090..33655
                     /gene="guaA"
                     /locus_tag="ACFIRM_000639"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC3028318.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVSDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            33717..34463
                     /gene="fabG"
                     /locus_tag="ACFIRM_000640"
     CDS             33717..34463
                     /gene="fabG"
                     /locus_tag="ACFIRM_000640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC3028319.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            34528..35013
                     /locus_tag="ACFIRM_000641"
     CDS             34528..35013
                     /locus_tag="ACFIRM_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC3028320.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPTVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(35231..36025)
                     /locus_tag="ACFIRM_000642"
     CDS             complement(35231..36025)
                     /locus_tag="ACFIRM_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3028321.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            36129..36854
                     /locus_tag="ACFIRM_000643"
     CDS             36129..36854
                     /locus_tag="ACFIRM_000643"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC3028322.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            complement(37408..>37758)
                     /locus_tag="ACFIRM_000644"
     CDS             complement(37408..>37758)
                     /locus_tag="ACFIRM_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC3028323.1"
                     /translation="FFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQE
                     LHYAKFLSQNSMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQA
                     ELEKVYGRRVILFK"
BASE COUNT         9439 a        11281 c         9059 g         7979 t
ORIGIN      
        1 ctatacaacc ccaccgcaca ccaacacaca aaggaaaccc catgacacgc ttcaaatact
       61 ccctgctttt tgccgccctg ctacccgtgt acgcgcaggc cgatgtttct gtttcagacg
      121 accccaaacc gcaggaaagc accgaattgc cgaccatcac cgttaccgcc gaccgcaccg
      181 cgagttccaa cgacggctac accgtttccg gcacgcacac cccgttcggg ctgcccatga
      241 ccctgcgcga aatcccgcag agcgtcagcg tcatcacatc gcaacaaatg cgcgaccaaa
      301 acatcaaaac gctcgaccgc gccctgttgc aggcgaccgg caccagccgc cagatttacg
      361 gctccgaccg cgcgggctac aactacctgt tcgcgcgcgg cagccgcatc gccaactacc
      421 aaatcaacgg catccccgtt gccgacgcgc tggccgatac gggcaatgcc aacaccgccg
      481 cctatgagcg cgtagaagtc gtgcgcggcg tggcggggct gccggacggc acgggcgagc
      541 cttccgccac cgtcaatctg gtacgcaaac acccgacccg caagccattg tttgaagtcc
      601 gcgccgaagc cggcaaccgc aaacatttcg ggctgggcgc ggacgtatcg ggcagcctga
      661 acgccgaagg cacgctgcgc ggccgcctgg tttccacctt cggacgcggc gactcgtggc
      721 ggcagctcga acgcagccgc gatgccgaac tctacggcat tttggaatac gacatcgcac
      781 cgcaaacccg cgtccacgca ggcatggact accagcaggc gaaagaaacc gcagacgcgc
      841 cgctcagcta cgccgtgtac gacagccaag gttatgccac cgccttcggc ccaaaagaca
      901 accccgccac aaattggtcg aacagccgca accgtgcgct caacctgttc gccggcatag
      961 aacaccgctt caaccaagac tggaaactca aagccgaata cgactacacc cgtagccgct
     1021 tccgccagcc ctacggtgtg gcaggcgtac tttccatcga ccacagcact gccgccaccg
     1081 acctgattcc cggttattgg cacgccgatc cgcgcaccca cagcgccagc atgtcattga
     1141 ccggcaaata ccgcctgttc ggccgcgagc acgatttaat cgcgggtatc aacggctaca
     1201 aatacgccag caacaaatac ggcgaacgca gcatcattcc caacgccatt cccaacgcct
     1261 acgaattttc ccgcacgggc gcctatccgc agccatcatc gtttgcccaa accatcccgc
     1321 aatacgacac caggcggcaa atcggcggct atctcgccac ccgtttccgc gccgccgaca
     1381 acctttcgct gatactcggc ggcagataca gccgctaccg cgcaggcagc tacaacagcc
     1441 gcacacaagg catgacctat gtgtccgcca accgtttcac cccctacaca ggcatcgtgt
     1501 tcgatctgac cggcaacctg tcgctttacg gctcgtacag cagcctgttc gtcccgcaat
     1561 tgcaaaaaga cgaacacggc agctacctga aacccgtaac cggcaacaat ctggaagccg
     1621 acatcaaagg cgaatggctt gaaggccgtc tgaacgcatc cgccgccgtg taccgcgccc
     1681 gtaaaaacaa cctcgccacc gcagcaggac gcgaccagag cggcaacacc tactatcgcg
     1741 ccgccaacca agccaaaacc cacggctggg aaatcgaagt cggcggccgc atcacgcccg
     1801 aatggcagat acaggcaggc tacagccaaa gcaaaccccg cgaccaagac ggcagccgcc
     1861 tgaaccccga cagcgtaccc gaacgcagct tcaaactctt caccgcctac cacttagccc
     1921 ccgaagcccc cagcggccgg accatcggtg cgggtgtgcg ccggcagggc gaaacccaca
     1981 ccgacccagc cgcgctccgc atccccaacc ccgccgccaa agcccgcgcc gtcgccaaca
     2041 gccgccagaa agcctacgcc gtcgccgaca tcatggcgcg ttaccgcttc aatccgcgca
     2101 ccgaactgtc gctgaacgtg gacaacctgt tcaacaaaca ctaccgcacc cagcccgacc
     2161 gccacagcta cggcgcactg cggacagtga acgcggcgtt tacctatcgg tttaaataag
     2221 gtcgtctgaa acggagtttc tgcgaagcta aaagcagcct gcacattgaa aatgccgccc
     2281 aagcaaacct gcagtttgcc gccctctccc tagccttctc ccacggggcg agggaattgg
     2341 ggtgcaggct gcctttaagg ttcaggcaaa ttttaacttc gttgatactg cgctttagct
     2401 tcgcagaagc tgcactttca gacgaccttt tggaacacca gaggtacacg catttaagga
     2461 atgccgtctg aagccgcctt caataccgcg ccatttccgc gtcaacctca accgcccacg
     2521 cgtcgatgcc gccctgaagg ttgtacaggt tttcaaaacc cgcctccgcc aaatacatcg
     2581 ccgtatgcag gctgcggata ccgtgatggc aatacaccac aagcggcaca tcgtccggca
     2641 actcgttttg ccgcagcgga atcagattca tcgggatatg cagcgcattt ggcagcgaac
     2701 aaaccgccgc ttcttcatcc gtacgcacgt ccaacaaaca aaacatccgc ccttcgtcca
     2761 tccacgcctt caatgccgcc gacggaagtt gcacaatatc catcacaaac cccccgaaaa
     2821 aaaccaagca aaatgccgtc tgaagcccca aacccgcttt cagacggcat cacaatccga
     2881 acatcaaaaa ttaaaactgt cgaaaggatg ggcatccttg tcgtccaaat gcgccaccaa
     2941 agtatcgaac agcacctttt cttcaaacac atcgcccctg cgcgtaatca aaagcgcgcg
     3001 ctgcaccggc ctgcgtccca caataaccgc catacgcccg ccgtccttca actgttcctt
     3061 taacacttca ggcacaaggg ttaccgcgcc gccgacgtaa accgcatcaa acggcgcacc
     3121 tgcggaaagt tcggtcaacc cgttattttg cacataatcg atgttttcca aagacaagcc
     3181 gtccaaaacc gctttggcgc gcttttgccg ttcggcatcg atgtcgtccg aaaccacacg
     3241 gccagccagt tttgccaaca gagccgtcgc atagcccgaa cccgtgccga tttccaaaac
     3301 cgtatcgttt ttcgtcagct tcaagccctg cgccagccgc gccacgactt tcggctcgag
     3361 catcttatga ccgttggcaa gcggcagctc catatccgca tacgccaaac cctgcaaaga
     3421 ctcatccgcg aaaagctcgc gcggaatctc ctccaaagcg tccaacacgt caaaatccaa
     3481 tacatcccaa ggacggatct gctgttcgac catattgaac cgcgcttttt caaaatccat
     3541 ttagtgctcc gtcaaataat tgttttatca atggcaggca ttataaaacc acactaccgc
     3601 cgcgccaact tcggcacgcg aagcccgccc gttttgccag tctcaagccg cctgacgcga
     3661 agcctcaaac cgcctctcca aaatcttcgc cagttcgccc aaatacaaag catctgcctc
     3721 atcaaactgc gccaaatgtt cgctgtccgc gtccaacacg ccgatacagc ggccgtctga
     3781 aaacagcggc acgacaatct ccgaacgcga caaagacgaa caggcaatat ggtcgggatg
     3841 cgcgttcaca tccttaacaa ccaccgtttc acccttcgcc caagcctgac cgcacacccc
     3901 gcgcccgaac ggaatccgcg tacacgccaa aggtccctga aacggtgcca aaaccaattc
     3961 gtccgaacgc gtatcgacaa aataaaaacc cacccacaac cagccgaacg cctccttcaa
     4021 aaccgccgcc gtgttcgcca aattcgccac ccaatccgcc tcgtccgcca ccacagactc
     4081 aatctgcggc aacacctcac gataaagcgc ggccttgtcc gaagccgaaa aatgaagcgc
     4141 gtgcatcata tctcccatat ttgcatacat atcaggcagc cattataaaa catcctgccc
     4201 gaaacaacat tccaaaccgc ccccgcagcc gcccgcccca agttgcgaac ccgccgcata
     4261 tccactaaac ttcacattgc aacgcgccac acgcggcaga cagaaaaaca cgacacggag
     4321 caaaaaagat gtatcaccaa atcggaatgt gggatcaaaa atgggtcatc ggcaactgga
     4381 aaatgaacgg ccggctccaa aacaacaacg cactgatgca ccgcttccgc atcctcccca
     4441 ccgccgaacg cgtcctcatc ggactcgccg ccccgaccgt ttacctgctg caactgcaca
     4501 acgccatgca aatcgtctta aacaaccgca tcctcacctg cgcccaagac gtgagccgct
     4561 tccccgacaa cggcgcgtac accggcgaag tgtccgccga aatgctcgcc gacaccggca
     4621 cagacatcgt cctcatcgga cactccgaac gcagccttta tttcggcgaa aaaaacgaaa
     4681 tccaacgccg caaaatggaa aacgtcctca acgtcggact catcccatta ttgtgcgtcg
     4741 gcgaaagcct cgaagagcgc gaaaccggca aagaacacga agtcatcgcc catcagcttt
     4801 ccatcctgca agggctggat accaaaaaca tcgccgtcgc ctacgaaccc gtctgggcaa
     4861 tcggcacagg caaagtcgcc accgtcgaac agattgccga tatgcacgca ttcatctaca
     4921 aagaaatctt gtctttgtgc ggaagcgatg ttaaaatccg cgtcctttac ggcggaagtg
     4981 tgaaagcgga caacgcggcc gacatcttcg cagtacctta tgtggacggc gcactcgtcg
     5041 gcggcgcgtc attgtcgtac gactccttta ccgccatcat cagtgccgca caaaatgcgt
     5101 agaaaaatat ggaagccttc aaaaccctaa tttggattat taatattatt tccgctttgg
     5161 ccgtcatcgt gttagtattg ctccaacacg gcaaaggcgc ggatgccggc gcgaccttcg
     5221 gatcgggaag cggcagcgcg caaggcgtat tcggctctgc cggcaacgcc aacttcctca
     5281 gccgctcgac cgccgttgca gcaacatttt tcttcgcaac ctgcatggct atggtgtata
     5341 ttcacaccca cacgacaaaa cacggtttgg acttcagcaa catacgacag actcagcaag
     5401 cacccaaacc cgtaagcaat accgaacctt ctgcccctgt tcctcagcag cagaaataac
     5461 agtttttcaa atgccgacat ggtgaaattg gtagacacgc tatcttgagg gggtagtggc
     5521 cgtaggctgt gcgagttcaa atctcgctgt cggcaccaac acacaaaaac gcttagaaat
     5581 tctaggcgtt ttttttcgtt taatcctgtc tgatgccatc aaccaatccc aatcaataca
     5641 aggatttcag gctgattgtt atcctgccgt ccccttcctg actgtgcaat cccgtccaat
     5701 ccgccctaat tgaactaacc tgatttttta cggtatccga aaaataccgc aaaaataaag
     5761 ctgaacgacc accaaatcag gaatgccaag cggaaaagag cttgcgggga atactgacga
     5821 accgtaggga acgaggggga aaccgtccaa gatgcagagc ggtttttttg ggtttttgga
     5881 aaaaacctat actaggaagc gataccctta gttgttacct tgttaccggg gaaaagttag
     5941 ataaataagt atatgaaata tagtgaatta aatttaaatc gggacaaggc ggcgaaccgc
     6001 agacagtaca aatagtacgg caaggcgagg caacgccgta ccggtttaaa tttaattcac
     6061 tataaaaata agaaaaagat aaaaaattgg taacaaatgc ggtaacaaat ggtaacgaat
     6121 cggtaacaac ttttggggtt ttccggtttt ttaccgtctt ggcagtggga gcgtagcgga
     6181 atgaaaagcc aaaacgcacg gaaccgcgcc tattttgagc aggaatggcg gttaaaccgg
     6241 ttggttatat tgcggggcat aggaagacag cgaaacgcgc taaaaacgca atttgagcca
     6301 ttaaaagcga ttgattaaaa aataatcaaa ttagccgccg gtgtttcagg cggcataaac
     6361 ggagaaattg cagggtataa aaaggcggct tgccgtgttg cctgtctttg gtataatccc
     6421 cagtatcact aagtaaacgg ttgcacaatg cggatattca aaaaccaatg gatagtgaaa
     6481 tttgccaaga agcacaaaat cagtgattca gagctgatag aggcggtaga gcgggcagat
     6541 aacgggctga tagatgcaga tttgggcggc gcgtgattaa gcagcgtata gcaaggcaag
     6601 ggcaaggcag aagcggcggt tatcgcagtc tgatattttt caggcatgga gaacgggcgt
     6661 ttttcatgac cgcctttgcc aagaacgacc gcgaaaacat cactgataaa gaacttacag
     6721 aattaaagaa aaccgccgcc atcatcttgg caatgacaga aaccgacata gagcaagcca
     6781 agtcaaacgg cactttcacg gagatacagc catgaaatat aaaagcggga ttttcgcagc
     6841 aatacacgaa accatgcagg gattgcacga catcggcgca atcgacaaaa agactatgcg
     6901 cggctttgac aagtcctgcc tgaccgaaat caagccgttg agcggcgggg acatcaaggc
     6961 aatcagggag aaggaggcac tatcgcaagc cgctttcgcc atctatctca acgtggggaa
     7021 aaatcacgtt tcggcttggg agcggggcgt taaaaagccc ggcggcgcag cgttgaagct
     7081 gctgaccatc gtcaaaaaca agggcatcga agccattgcg tagccgactt ggcaaacggc
     7141 aaaatcagca agttcacata tagacgcgct gctgaatatg cctgccaaga caggcaagac
     7201 cgccgaactg aatatcaggg cgtagccgca caaatgcccg accgcatcaa accaagccga
     7261 aacggcggcg gtacagacgg catagcccga cagcaaggca cggcgcagac gggcgcgaaa
     7321 cccgaaacat ccccgaccgc gaggcacggg gattttttgc gcccgttgca ggggggggat
     7381 tggatttaag cggcgcgggc ttgaaggcaa aacgggcggg gcgcagaact gtttaaatgc
     7441 cgtctgaatc tcaaacgatt tcagacggca ttttgaaaca acggctcaaa ttctcgatcc
     7501 ccttccctta acgccgacgt tttttattaa cgcgcccctt atttctgaca ctttgctcat
     7561 aaaccggcat aacggtcggc aacaaccgtt ttagattttc tatacgggca ttgtttacag
     7621 catcagcata agacaccgta ctctggaata ccgcctaaac cctgcggata gttttggatg
     7681 tggtatcgac attgtcggca gacttgtcta actcgaccgt tttcaaataa tctttggcag
     7741 atgctgcatt cagtgtcgcg gaatccgcgt attttgaaca aaaggtcggg ctactttatc
     7801 acaatagcag aaagatggaa aacggcgggg attggtgaaa ggtcgtctga aatgatattc
     7861 agacgacctt gaacggttgt gttttaaata tggtatttat tcatttgttt caccatcagg
     7921 cagtccaaac caccttaagg gaatcgtgtt aaattggtaa tggcagttaa cactgataat
     7981 ataatctaaa acatctttaa tattccggtt ttgtaaacca atcattcaaa acatagacat
     8041 atccaacccg ccaacctaaa gagtgaacta actttgtata ttgttttctc ttaaaatcgc
     8101 aagtttgtaa tttctcatct actgaaccgg gagtttgttg gaatttgatc tcaataataa
     8161 ataaggtatc acggatgatg ataaataagc cattatcggg cagtaatctt ttagacagat
     8221 ggtctttcca attaatattg tattccagtc tttccaaaaa tcggtaaaac tcatattttt
     8281 tgaaacaata agccttcaat tctccattaa accaaacttc ataacccgca ttatcatctg
     8341 tttttcttaa gtcgtacccg ggaatttctt caaacaactt tcgtaagtct gtacgttctt
     8401 cgaatctgag tcctgtttgt gtccttgcac cgccgattcc gccaataatc atttttctaa
     8461 ccttttcttt gctaattcaa aaaatcttct tccagttcgc aaccgcaaaa tctccgacca
     8521 tgttttaagg cggcaacgcc tgttgtgccg ctgcccataa aagggtcaaa aattaaatct
     8581 ccgatatttg aagccgatag tatgcagcgt tcaagtaagg agagtggttt ttgtgtcgga
     8641 tgtttgccga atgttttttc ggttttattt ggtggaggaa atgtccaaac acatttcatc
     8701 tgtttgccat tattttgtgc tttcatcatt tcataatgaa acgtatgttt ggctttttta
     8761 tttttctttg cccataagat tgtttctgtc gaatgggtaa agaaacggca ggacaaatta
     8821 gggggaggat tgggtttttc ccaagtaata ttgttcaaaa tatggtagcc gacggtttgc
     8881 atcaggtagc cgattaaata gatattatga aatgtgccgc aaacccaaat tgtgccgttt
     8941 ggttttaata aggcgtaaca cagtcggagc cattcttcgt aaaattccaa atctgccgcc
     9001 attcctttgg atttatccca gttgccctta ttgacggaaa ccatttgccc gttttgacaa
     9061 ctgaaaccgt cattggaaag aaagtaaggc gggtctgcga aaatcatatc aaaacagccg
     9121 tttggatgtt tttccaatat cttccgcatg acgttaaggg aattttcgtt gtagaggaca
     9181 aggtcgtctg aaacgatttt ttcaaacgac tttgataaat ggcagcttct tcagccggtt
     9241 tgtcaaaatc aatcataaat tgccaataat tctccgactt ttttacgact gctgcccttt
     9301 gcggcaatat tgcgttgtac ttcaaccgtt tttagaatgg cagaagaata aatatttctc
     9361 gtaaattcag tatcatgatt tgaaatcaaa acgccttggg agttgggtgc aatttgtcga
     9421 tttttttgta aagtccgcga ccaatgaatt cgatcatatt ttgatcacgc aaaatttgca
     9481 actgttggcg gattttgtct ttgatatggt tgttttgggg aaattggatg gatagtttgt
     9541 tttcaaattc atacatttgc gacaatgtga attcttcggt gagttggtcg atacatttca
     9601 taacagccaa aagccagtct ttgcgctccg cattttggtt gcgtaaaaac aggttggatt
     9661 gccatttttt cagaacggtt tcgggttcga taatgcggga atcgtctatt aagaatattt
     9721 ttccgctttc aggcaaaggg gcgagattga tggaacacat aatgtggtgc ggtcggtttt
     9781 taatgccttt atttctggga ataatcatat ccggcgtgat gaaatgtttg ggtacaagca
     9841 ccaattgccg tatggagtaa tccgcttttt tatatgcaag aaagaaaaag ttggggttgg
     9901 tatctgaccg gatgcgctcc aacatagtgc gatatgcacc gtcaggcacg ctgttgccta
     9961 tggttttttg atttttactc tttaattcat attgctcgtg gcaatttggg caaaagaggt
    10021 ctgcaacagg tttgttattg gcaaatctct gcatcggctt gcttccgcaa caggggcaat
    10081 agccgttttt ttccagccaa gcctcgctca ttacacggat tttgtgggtt gctttatttt
    10141 gttgctttcc caattgggta tcgaaaaata aattcatatt ttggattttg agattaattt
    10201 ttagtagatt aacaaaaatc aggacaagga gacgaagccg cagacagcag ggcaaggcgg
    10261 ggcaacgccg taccggttta aagttaatcc gctatatttg aggtctgtct tgcagacaac
    10321 ccaatctgcc ttaaaaaggc cgtctgaaac cctgtttcca agtttcagac ggcctttatc
    10381 cgtatggcta aaccttaaaa gcggtttaga cgacgatgtt caccagtctg cccggtacga
    10441 cgatgatttt cttcgcaggc ttgccttcca tgaatttcac tgcgccttcg ttggcgagtg
    10501 cggcggcttc gaggtcggct ttggaggcgt cggcggcgac ggtgattttg ccgcgcagtt
    10561 tgccgttgac ttgaaccatc acttcgattt cggatttgac cagggcggct tcgtcgactg
    10621 tcggccagcc tgcttcccac agtttcgcgc cgttcaattc gctccacagg gtttcgcaga
    10681 tgtgcggcac gatgggccac aacaggcgta cggcggcttc caatacttct tgggcgacgg
    10741 cgcggccttg ttcgctgccg gtgtcggttt tgtcgtattg gttgagcagt tccatcacgg
    10801 cggcgatggc ggtgttgaac tgctggcggc ggccgtagtc gtcgctgact ttggcggtgg
    10861 tggaatgcag tttgtggcgc aggtctttga gttctttaga caaaccgtct tggttgcctg
    10921 caaatgcttt gaccgcgccg ccttgcttca ggtattcgta aacggtacgc cacagacggc
    10981 gcaggaagcg gtgtgcacct tcgacgccgc tgtcgctcca ttcgagggac tgttcgggcg
    11041 gtgcggcgaa catcatgaac aggcgggcgg tgtccgcgcc gtaggcgtta atcagttctt
    11101 gcggatcgac gccgttgttt ttggatttgg acattttttc cgtgccgctg atgacgacgg
    11161 gcagtccgtc ggctttgagg acggcggaaa cggggcggcc tttgtcatcg aaagtcagct
    11221 cgacatcggc ggggttgatc cagtctttgc cgcctttgtc gttttcgcgg tagtaggttt
    11281 cgcagacgac cataccttgc gtgagcaggc gttcaaacgg ttcgtcaaca ttgaccaaac
    11341 cttcgtcgcg catcagtttg gtgaagaagc gcgcgtacag gaggtgcaaa atcgcgtgtt
    11401 cgatgccgcc gatgtattgg tcgaccgcgc cccagtattt cgcggattct gccgatacca
    11461 tgccgtctga aaacttgggc gacatatagc ggaagaagta ccagctcgac tccatgaagg
    11521 tgtccatggt gtcggtttcg cgtttcgccg cgccgccgca gcacgggcag gaagtttcgt
    11581 aaaactcggg catttttgcc agcggcgaac ccataccgtc gggtacgacg ttttcaggca
    11641 ggacgacggg cagttggtcg gcagggacgg gaacgtctcc gcatttttcg caatggacga
    11701 tgggaatcgg gcagccccag tagcgttggc gcgaaatgcc ccagtcgcgc aggcggtatt
    11761 gggttttcgg ttcgcccgca ccttggcttt gcagcttggc ggcaacggca tcaaatgccg
    11821 tctgaaaatc caagccgtcc aagtcgccgc tgttgaccaa tacgccgttt tctttgttgc
    11881 cgtaccattc ttgccatcgg tttgcgtcga atgcgttgtc gccgacggca atgacttgtt
    11941 ttttcggcag attgtatttg gcggcaaact cgaaatcgcg ttcgtcgtgc gccggaacag
    12001 ccatcaccgc gccgtcgccg tagccccaca agacatagtt ggcaatccac acttccagct
    12061 tgtcgccgtt gagcgggttg acgacgtagc ggccggtcgg cacgcctttt ttctccatcg
    12121 tcgccatatc ggcttcggca accgaaccgg ctttgcattc ggcgataaat gcctgcaatt
    12181 cgggtttgtc ggcggctgcg gctgttgcca gcggatgttc ggcggcaaca gcgacataag
    12241 tcgcacccat cagcgtgtcg gggcgggtgg tataaacttg caggaatttc gcgtaatcgc
    12301 cttccaaacc ttgcttgctg tcgtctgaaa cggcgaagcg cacggtcatg ccgcgagatt
    12361 tgccgatcca gttgcgctgc atggttttga cttgttccgg ccagtgttcc agcttgtcca
    12421 aatcgttgag cagctcttcg gcgtaatccg tgattttgaa gtaatacatc gggatttcgc
    12481 gtttttcgat caacgcgccc gaacgccagc cgcgtccgtc gatgacttgc tcgttggcaa
    12541 ggacggtttg gtcgaccggg tcccagttca ccgtgccgtt tttgcgatag acgatgcctt
    12601 tttcaaacag cttggtaaac agccattgtt cccagcggta gtattcgggt ttgcaggtgg
    12661 cgacttcgcg ttcccagtca accgcaaaac ccaggctttt gagctgggtt ttcatgtatt
    12721 cgatgttgtc gtaggtccaa gcggcggggg cgacgttgtt tttcatcgcc gcattttccg
    12781 ccggcatgcc gaacgcgtcc caacccatag gctgcataac gttgaagccg tttaaaagtt
    12841 tgaagcggct caatacgtcg ccgatggtgt agttgcgtac atgccccata tgcagcttgc
    12901 cgctagggta agggaacatc gaaaggcaat aatatttggg tttggaagcg tcttcggaga
    12961 cgttggaaat acgggcgtcg tcccattttt tctgcgccgc aggctcgatg gcggcgggct
    13021 ggtaatgttc ttgcatggtc attttgtttt tcgcttgaaa acgttaggaa tttaaaggag
    13081 gaattataac aggttgccga tgtcggcgaa tcggcaaatt gccggcagga tgcgtaaatt
    13141 cgcacgcgca tcattccgta tgccgtacaa atacaccgcg tttactgata cgcacgtttt
    13201 ttatgctaat attacgaacc aaaatcaaac gtttaaaact ctcctgatgc ggctcttccg
    13261 aacaaaaggc agacgggcat cgggtaaaaa aggattctgc atatgaaaat caaacaaatc
    13321 gtcaaaccga gcttggcagt attggcggca ggcattctgt ctgcctgcgc aaccataaag
    13381 caacgtcaaa gccgacggca cgaccgacaa tccggttttc ccggaaccct attccgtaac
    13441 gctcgacaac aagcgcggca cattcccgac ttatgacgaa ctggatcaga tgcgccccgg
    13501 tctgaccaaa gacgacatct acaaaatccc gggccgcccg cattacgacg aaggtatgta
    13561 cggcgtgcgc gaatgggatt acctgttcca cttccacacc ccgggcgtgg gtatcgaccc
    13621 tgaaaacact tccggcgtgg aaggcgttac tacctgccaa tacaaagtga ttttcgataa
    13681 agacaaattt gcccgcagct tctactggaa ccccgtcttc ccgaaagatg ccgtctgccc
    13741 gccgcccgca cccaaagccg agccgcaagt catcatccgc gaaatcgtgc cggcaaaacc
    13801 gaaacgtatc cgccaataag ccgctatgcc gccccgcctg ctttcaggga tattgtgctg
    13861 cctgctgacg actgcccccg tattcgcaca ggggcagccg gatgcggtca gtgcctatat
    13921 tcaaaagaaa aaagtcatcg tcgacacatc caaggcggaa ctctgttttg ccgacgaccg
    13981 tcagtgccac cccgtcctca tcggcactgc cacacccaag gggacgttcg gactgacgct
    14041 gaacagtacc gacaagcccg gttacggcgg cgaagtcatc ggtttcaagc aggagggcga
    14101 ttttcttttc gccctgcacc gcgtttggaa tcagataccg tccgaaaggc gaaacgaacg
    14161 catcgcctcc ccgtccgtgt ccgacaggat tatgaccaac ggctgcatca acgtcagcga
    14221 tgcggtgtac gaaaaactgc gtcattattt tgtgttggaa gtgatttgaa acagacggat
    14281 accgcacgta ccggtatccg ttttcacatt gccccgatgc ctgaaacaga ccgtccgtca
    14341 cgtcatgccg cctgaaaccg gcgcagatgc cgccaagcct tcagacggca ttgtccgccc
    14401 gctccgaccg aacaacaacc atctttggga gaaccttatg accgaacaaa accgcatcct
    14461 ctgccgcgaa ctgagcctgc tggcattcaa ccgccgcgtg ttggcacagg cggaagacaa
    14521 aaacgtcccc cttttggaac gcctgcgctt cctgtgcatc gtgtcgtcca atcttgacga
    14581 gttttttgaa gtccgcatgg cgtggctcaa gcgtgaaaac aagctgcacc cccggcgcag
    14641 gccggacaac ggcaaaatgc cgtctgaaac catcgccgac gttaccgaag cggcgcgctc
    14701 cctgatacgg caccagtacg acctgttcaa caacgtcctt cagcccgagt tggcgcggga
    14761 aagcatccat ttttaccgcc gccgcaattg gacgggcacg cagaaaaaat ggatcgaaga
    14821 ctatttcgac cgcgaactgc tgccgatcct gacccccatc ggactcgacc cttcccaccc
    14881 tttcccgcgc ccgctgaaca aatcgctcaa cttcgccgta gaactcgacg gcacggacgc
    14941 gttcggcagg ccttcgggta tggcgattgt ccaagcaccg cgcatcctgc cgcgcgttgt
    15001 tcccctgccg tccgaactgt gtggcggcgg acacggcttc gtcttcctgt cgtccatcct
    15061 ccacgcccac gtcggcaaac tcttcccggg catgaacgtc aaaggctgcc accagttccg
    15121 cctcacgcgc gacagcgact tgaccgttga cgaagaagac gtacaaaacc tccgcgccgc
    15181 cattcaaaac gaactgcacg accgcgaata cggcgacggc gtgcggctcg aagtcgccga
    15241 cacctgtccc gcctacatcc gcgactttct gctcgcgcaa ttcaggctga ccgacgccga
    15301 actctatcag gtcaaaggcc cggtcaacct cgtccgcctc aacgccgtcc ccgacctagt
    15361 caaccgcccc gatttgaaat ttccgccgca cacgccgggc cgtctgaaag ccttgggcaa
    15421 aaacagcccg atcttcgatt tggtgcgcca atcgcccatc ctgctgcacc acccctacca
    15481 atctttcgat cccgtggtcg atatgatacg cgaagccgcc gccgatcccg ccgttcttgc
    15541 cgtcaaaatg acgatttacc gcaccggcac gcgctccgaa ctcgtccccg ccctgataaa
    15601 ggcggcactc gccggcaaac aagtaaccgt cgtcgtcgaa ctgatggcgc gttttgacga
    15661 agccaacaac gtcaactggg cgaagcagct cgaagaggcg ggagcgcacg tcgtgtacgg
    15721 cgtgttcggc tacaaagtcc acgccaaaat ggcactggtc atccgccgcg aagacggcgt
    15781 gctcaagcgt tacgcccacc tcggcacggg caactaccac caaggcacat cgcgcatcta
    15841 caccgacttc ggcctcatta ccgccgacga acaaatcacc gccgacgtga acacattatt
    15901 tatggaaatc acaggcttgg gcaaacccgg gcggctgaac aaactctacc aaagtccgtt
    15961 taccctgcac aaaatggtta tcggccgcat cgctcgcgaa accgaacacg caaaagccgg
    16021 caaaccggcg cggattaccg ccaagatgaa ttcgctcatc gaaccgactg tcatagaagc
    16081 cctgtatcaa gcaagcgcgg caggcgtgca aatcgatttg attgtgcgcg gtatgtgtac
    16141 cttgcgcccg ggtgtaaaag gcttgtccga aaacatccgc gtccgctcca tcgtcggcag
    16201 gcagctcgaa cacgcgcgcg tatattgttt ccacaacaac ggcgcggacg acacctttat
    16261 ttccagcgcg gattggatgg ggcgcaattt cttccgccgc atcgaaaccg ccaccccgat
    16321 taccgcgccc gaactcaaaa aacgcgtcat ccgcgaaggt ttggaaatgg cactggcaga
    16381 caatacccac gcgtggctga tgcagcccga cggcggctat atccgcgccg cgcccgccga
    16441 aggcgaaagc gaagccgacc tgcaaaacga tttatgggat ttgctccgag gctgacccgc
    16501 acaagcactt cccccatacg ctcccaaccc caatccgccg cattccagac ggcattgcct
    16561 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
    16621 gggcatcgtg ttccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
    16681 ggattgttcg gcacggtaaa cagcgtcgag cggttggcgt cgccgaaggc aagctgcata
    16741 tcgtcggaat ggatgttgcg caacacgtcc atcaaatagc cgatattgaa accgacttcg
    16801 agttcgccgc cctgataggc gatttcgatt tcttcgcgcg cttcttcctg ctcgttgttg
    16861 ctgcacacga cgctcaacag gccgggttgc aggaacaggc gcgcgccacg gaatttttcg
    16921 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caacacgaaa
    16981 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
    17041 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gcttgtccag cagctcgatt
    17101 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
    17161 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
    17221 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
    17281 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
    17341 aaacgctcct gccccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
    17401 aaatcggcgg caggcagggt ttgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
    17461 cggttgtcgt cccaatccag cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc
    17521 tgaaatttct tggcattggt agtgatgcgg aagtcgcccg cacctccctc gggacccgcg
    17581 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc cctgccctca
    17641 atcagcacat tggacaggat gggcagggtg tgtcggcgtt cgacgatgcc ggtaacggct
    17701 tgcagcggct tgagcaggct gtcgcgctcg gcttgtaaaa tcaacatttt cgctccttaa
    17761 atcggttgga tttcctaccc ttcggacggc atggtcaatg tcatgccgtc tgaaaggcgt
    17821 gtccggtcag ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc
    17881 ttcctcgcgc agtttcgcca ccgccctgat gccgtgcatg acggtcgtat ggtcgcgtcc
    17941 gccgaacgaa tcgccgatag acggcaggct caaagtggtc aattctttgg tcaggctcat
    18001 ggcaacctga cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat
    18061 tttgatgcgg taatatttcg ccaccgcatc gatgatgatg tcggcggtga tgactttgtg
    18121 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
    18181 gttcataaag cggctgctcg ctccgacacg gttgaacgcg ccttccagtt cgcgcacgtt
    18241 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcgatactga tgcccgccgc
    18301 ttccgccttt ttctgcaaaa tggcgatacg catttccaat tcgggcggct cgagttccaa
    18361 agtcagcccc cacgaaaagc gggatttgag gcggtcgtcc ataccttcga ttttggcggg
    18421 taaaacatcg caagtgagga tgagctgttt tttctcattg tgaaaatggt tgtacagata
    18481 gaaaaattct tccatcgtac ggtcttttcc tttgatgaac tgaatatcgt cgataatcag
    18541 caggtcgtat tgcttgtatt gctgcttgaa cacgtcgtag gtattgttgc gaaccgcctt
    18601 cataaagctg cggatgtagt cgtccgaatg catatagcgc actttggcat cgggacggtt
    18661 tttcaacagc tcgttgccga ccgcctgcac aaggtgggtt ttgcccaaac ccgtgctgcc
    18721 gtacaggaag aacgggttgt aactctgccc cgggttttcc gcaatcgcct gcgccgcagc
    18781 cgccgcaagg cggttgccct taccttctac caacgtatca aacgtgtaat ccggagacag
    18841 gttggtttgt tcgtaacgcg cttcttccgc atcacgctgc gcatccgtcc gcgctttggc
    18901 aactgccgcc gattccggcc gggaagcagg cccggcagcc tgacgcggct cgtgcggcag
    18961 gtttttcata cgttccgcca aaatatccgc cgccgttttc gacgcagcgg gtttgacagg
    19021 cttttcagac ggcagctcgt ccaacagaac ctcctgcacg ggcatctcct ccgaccccgc
    19081 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
    19141 gccttctccc ggtttgaata cgaaggcggg acggccggca gccaactctt ccctcaccgc
    19201 ttctattttt ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta
    19261 caccacccat acgccaccct cctcaccaac cgtaaggggc gcaatccatt gcgcaaactg
    19321 cccgtgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
    19381 catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
    19441 aaaagaaaac aaatctgttg aatacgcgtc ggttcaaaac gcagcgaacc gattataccg
    19501 actcgccgat attttatcca caacccgtgc aaaaatttat ccacagaaag gcggcagaaa
    19561 tccgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
    19621 attggagtgt aattcacggt ttaattatct acccattcta ttttaggaac catcatgaaa
    19681 cgcacttatc aaccttccgt taccaaacgc aaacgcaccc acggcttcct cgtgcgctcc
    19741 aaaacacgcg gcggacgcgc agtattggcc gcacgccgtg ccaaaggccg caaacgcctg
    19801 gcagtgtaat tttggactac cgcttcggaa ggcagtaccg cttgttaaaa acggatgatt
    19861 tttcatccgt ttttgcattc agaaaccgcc gcagccgcga cctgctgcaa gtttcgcgct
    19921 caaacggcaa cgggctggac catccccgca tcggtctggt ggtcggcaaa aaaaccgcca
    19981 aacgcgccaa cgagcggaac tatatgaaac gcgtcatccg cgactggttc agattgaaca
    20041 aaaaccggct gccgcctcag gatttcgtcg tgcgcgtccg ccggaaattc gacagggcta
    20101 ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt cggcaacccc gcaaccggat
    20161 gcgggaaaca ggtatgatca gaacggtacc ctgcaggcaa ggttcagacg gcaacgggtt
    20221 tcccatacaa ggaacatccc gatgaacttc ctattgtcca aactgctgct ggggctgata
    20281 aggttttacc aatactgcat cagcccgctg attccgccgc gctgccgtta tacgccgacc
    20341 tgttcgcaat acgcggtcga agcggtcaaa aaatacggcg cattcaaagg cctccggctc
    20401 gccatcaagc gcattgcacg ctgccaccct ttcggcggac acggacacga ccccgttccc
    20461 tgacccgacg caatattcaa attgcacgct ttccttttat ttcccatcgg tttctatata
    20521 atgccgtctg aaccttcggg ctggcggcac ggccgtcggg tatgaagccc gcccttattc
    20581 cccgtctatc ggaacacgca acctgcggca tttccgacca ttcaggaaac tctatatgga
    20641 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
    20701 aatgttcccc accccgaaac ccgtccccgc gccccaacag gcggcacaaa aacaggcagc
    20761 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
    20821 ggttcaagcc gttattgatg aaaaaagtgg cgacctgcgc cggctgaccc tgctcaaata
    20881 caaagcaacc ggcgacgaaa acaaaccgtt cgtcctgttt ggcgacggca aagaatacac
    20941 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctga aaggcatcgg
    21001 ctttagcgca ccgaaaaaac agtacaccct caacggcgac acagtcgaag tccgcctgag
    21061 cgcgcccgaa accaacggac tgaaaatcga caaagtctat acctttacca aagacagcta
    21121 tctggtcaac gtccgcttcg acatcgccaa cggcagcgct caaaccgcca acctgagcgc
    21181 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
    21241 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
    21301 cttggacgac gatgcgaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
    21361 cggttggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacctaaagg
    21421 cggccaaagc gtttgcgccg caggcgactg ccgtatcgac atcaaacgcc gcagcgacaa
    21481 gctgtacagc gcaagcgtca gcgtgccttt agccgctatc caagccggcg ccaaagccga
    21541 aactgccgtc aacctgtatg ccggtccgca aaccacatcc gttatcgcaa acatcgccga
    21601 caacctgcaa ctggcaaaag actacggtaa agtacactgg ttcgcatcgc cgctcttctg
    21661 gctcctgaac caactgcaca acattatcgg caactggggc tgggcaatcg tcgttttgac
    21721 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcctaccgtt cgatggcgaa
    21781 aatgcgtgcc gccgcaccca aactgcagac catcaaagaa aaatacggcg acgaccgtat
    21841 ggcgcaacag caagcgatga tgcagcttta caaagacgag aaaatcaacc cgctgggcgg
    21901 ctgtctgcct atgctgttgc aaatccccgt cttcatcggc ttgtactggg cattgttcgc
    21961 ctccgtagaa ttgcgccagg caccttggct gggctggatt accgacctca gccgcgccga
    22021 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cctatctgaa
    22081 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
    22141 cgtcatgttc ttcttcttcc ctgccggttt ggttctctac tgggtggtca acaacctcct
    22201 gaccatcgcc cagcagtggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
    22261 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
    22321 tttattgttc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
    22381 cgggcgtttt ttcagattgg gcatttcctt catcctgcgc caatccgcaa ccgttttact
    22441 gaccaccaac tgagtcggca acgtcaaatc cgtagccgta cacaagcgcg tttcaggatg
    22501 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
    22561 ctgcgtctcg ccgcactgcc gcgaacgttg ctccaaagca ttcaaactct gaatgcgctc
    22621 atttttttca gacggcagat aaccattaaa cgcaaagttc tgcccattcg cacccgaagc
    22681 catcagcgcc agcagcaggc tggacgggcc gaccaaaggc cgtacttcaa aaccgtgttt
    22741 atgcgccaat gccaccaaat tcgcgcccgg atcggcaaca gccgggcaac ccgcctcgct
    22801 gacaatcccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
    22861 atccgtgtgt tcattcaacg tttgcagatt cagttcgcgt atcggcgtcg ttatccccaa
    22921 atgcttcaaa tgcgcacgcg ccgttttttc cgcttccacg acaaaatccg tcagcccgac
    22981 aaccgcccgt tgttcatgct gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
    23041 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtccgaa
    23101 cccttcagac ggcataaacg ggcagttaca aaacatccac gccctcgttt ttcaaaaaat
    23161 cgaccagacg gaaaaccggc agaccaatca aggcattcgg atcggtactt tcaatccgtt
    23221 cgatcaacag cgcgcccaag ccctcgcttt tcgccgcaca cgaacaataa accgcatccg
    23281 gctcgcgctc caaatagcgg aggatgtgca gctcgtccaa ctgcctcatc acgaccaccg
    23341 tcttatcgat atgccgatgc atcctgcccg taaccgtatt tagcagaacg accgcactgt
    23401 aaaactcaat ctccctgccg ctcaaatgca tcaacatctt ttgcgcgttg gcaaggttca
    23461 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
    23521 ccccggggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
    23581 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggatgcc gcctgaaagg
    23641 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaaccaaag
    23701 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcggtaaa
    23761 atccagccat gcgccgaaac acggacgccc gccttcggcg taccgcaaca tttttcttaa
    23821 aaatattgac gttagaacat ctaaattata tcataccccg tttatgtcag accctaattt
    23881 gattgacccg gaaatttttg ccgccgaaag gcagaacctg caaggcagtt ttctgctgga
    23941 agaattggac gagcgagtca gtttgcacga ttatcccgcc gacaggcgga ccaaaatatc
    24001 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
    24061 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgctcgatga
    24121 aagcagccgt atcgtcctgt tttccgacga agagtccttg gacgaatcca tgcttgccga
    24181 caaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcgcat tggtagaaga
    24241 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgga cactgcggca atacccttcc
    24301 ggaatccgcc aaccaagaca aacccaaccc ctttgctgtt ttggcgggtt tgaaaagcag
    24361 ttaattagga cacagtttat ttatctagga gcttgaaatg gccgttcaac aaaacaaaaa
    24421 atccccttcc aaacgcggta tgcaccgttc gcacgacgca ctgaccgcgc ccgcactgtt
    24481 tgtcgacagc acaaccggcg aagtacaccg cccgcaccac atctccccca acggtatgta
    24541 ccgcggccgc aaagtggtca aagccaaagg cgaataatcc ctattcgact gactgaaaaa
    24601 gccagaacat tgccatgcaa ttactggctt tttttgcatt ggacgcacca tccgtccaaa
    24661 ctttcgccat acgtcaacac acaggggcaa agcgttccgt ataatacccc gtgaaaatat
    24721 tccaaaagcc ccaaccacca aggaaattcc gatgaaacag aaaatctggt acacctacga
    24781 tgacatccac cgcgtcatca aagcattggc agaaaaaatc cggaacgccg gcgtcaaata
    24841 cgatgccatg atcgctatcg gcggcggcgg ctttattccg gcacgcatgc tgcgctgttt
    24901 tctggaaatt ccgatttatg ccgtaaccac cgcctattac gacagcgaca acgaaggaca
    24961 agttaccgaa gaagtcaaaa aagtccaatg gctagacccc gtccccgaag tcctgcgggg
    25021 caaaaacgtg ctcgtcgtcg atgaagtgga cgacagccgc gtaaccatgg agttctgcct
    25081 gaaagaactg ctcaaggaag acttcgacac tgtcggcgtc gccgtcctgc acgaaaaaat
    25141 caaagccaaa gcaggcaaaa tccccgaagg cattccctat ttcagcggca ttaccgtaga
    25201 agactggtgg atcaactatc cgtgggacgc actcgacatc gacgaacaca accgccttgc
    25261 cgaagccggc cgaggctaac cctttcagac ggcatatttt ccgaaccgat gccgtctgaa
    25321 gcccgcacga cccctgccgc agaccgaaaa tctaccggag aaacccctat gattacattg
    25381 gccgtagatg ccatgggcgg cgaccaagga cttgccgtta ccgtacccgg cgcaaccgca
    25441 ttcctccaag cacaccccga tgtccgcctg attatgaccg gcgacgaaac gcagctccgc
    25501 caagccctga acgcggcagg cgcgccgatg gaacgcatcg acatctgcca taccacccag
    25561 gttgtcggca tggatgaatc ccctcagtcc gccctaaaga acaaaaaata ctcctccatg
    25621 cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag ccgccgtatc cgcaggcaac
    25681 acgggcgcgc tcatggcaac cgcacgtttc gtcctcaaga ccatacccgg catcgaacgc
    25741 cccgccatcg ccaaattcct tccttccgac accgaccacg ttaccctcgc cctcgacctc
    25801 ggcgcaaacg tcgactgcac acccgaacag cttgcccaat ttgccgtcat cggcagcgaa
    25861 ctcgtccacg cgctccatcc tcaaaaagga cagccgcgcg tcgggctggt caacgtcggc
    25921 acagaagaca tcaaaggtac ggataccgtc aaacaaacct acaaactgct gcaaaacagc
    25981 aaactcaact ttatcggcaa catcgaaagc aacggcatcc tgtacggcga agcagatgtc
    26041 gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa ccatcgaagg cgcagtcaaa
    26101 ttcatgagcg gagccatccg ccgcgaattc cagagcaacc tgttcaacaa acttgccgcc
    26161 gttgccgccc tacccgccct caaaggcttg aaaaacaaac tcgatccgcg caaattcaac
    26221 ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa gccacggcgg cacagacaaa
    26281 accggtttcc gctacgcact cgaagaagcc taccacgaag ccaagtccgc cggcctttcc
    26341 aaaatcgaac aaggcgttgc cgaacaactc gccgcactgg aagccgccca aaacgaaacc
    26401 gccgccagtc tgtaacacgc acgatgccgt ctgaacgccc ccgccccttt cagacggcat
    26461 ccggcacccg tccaaacctg cgggcgcgga cggcgatgcg cctgtccggc acttcccaaa
    26521 tacttccctg taaaataagg agtatttgaa aaatgaagac attagaaaaa cggatgaaag
    26581 ctctagacaa acggatgatg aagttcggaa aatcccttga aggcaggctt gatgcccgtc
    26641 tgattgaatc cgcattggat tatattcatt attcggaacg ttttttggct tttgacatcc
    26701 tgtgtactta tatcgaagat ttcgatatcc ggctgacgga acaagaatcc cgggaaattt
    26761 cttttatcaa caaggaattt gagatagaaa gcacgtccga ttaaccaata aagccaatga
    26821 tttgataaga atgaaaacat cgacggtcgt ttttggcgga ttttttatgg cagacaacgg
    26881 ggagcgaatc caaatccccg ttttggaaaa tcctgacatt agggaaatca atcgcttttt
    26941 ttccgtatca aattttgaga aaaaagccgg cgtccttgtt ttcagaatca tccccgagcc
    27001 ggaatttggc aataccgaat taactgtcta ttttgaaaaa ggatattata gtggattaac
    27061 aaaaaccggt acggcgttgc cccgccttag ctcaaagaga acgattccct aaggtgccca
    27121 agcaccaagc gaaccggttc cgtactatcc gtactgtccg cggcttcgcc gccttgtcct
    27181 gatttttgtt aatccactat atttgccaat aattcaaaca attttagaag gcggggatat
    27241 taaagtaaaa aaccttaaaa cagaaaatta cagcgggaag gcaagggaaa tcttgggaga
    27301 ttcctaccct attgaacata tctctaaaaa tatttctaca attcaaaata tcatttccga
    27361 atttatcaga caaaaacaaa caccggcacc aaataatttg atggaaaccc aacaatcaag
    27421 atgccgtctg aagcagcttg ggctttcaga cggcatttcc ttcgcttaaa acagcgtatc
    27481 ggcaaccccg ccccgcccgt ccacggcaat ccgcatctga aaaccatctg tatgccaaac
    27541 cacaccccca tccctgtttc cgtcatttgc accctgcccg tattgggcaa tcatcggttt
    27601 ttcgcttaca atagccgaat ctgaaccaac tctctaaaaa ggccgttccc atgcagtatg
    27661 caaaaatttc cggcacgggc agctatcttc ctgccaaccg cgtcagcaat gacgaccttg
    27721 cccaaaaggt agatacctct gacgagtgga ttaccgcgcg tacgggcatc aaattccgcc
    27781 atattgcagc cgaaaacgaa aaaaccagcg atcttgcagc cgaagcggcg cggcgcgctt
    27841 tggcagatgc caaattaaat ataaacgaca ttgatttgat tatcgtggcg acggcaacgc
    27901 cggatatgca gtttccttct accgccacca tcgtgcagca aaaattgggc atcaccaacg
    27961 gctgccccgc gtttgacgtg caggcggtgt gcgcaggctt tatgtacgcg ctgactacgg
    28021 caaatgccta cattaaaagc ggtatggcga aaaacgcgct ggtcatcggc gcggaaacct
    28081 tcagccgcat cgtcgattgg aacgaccgca caacctgcgt gctgttcggc gacggcgcgg
    28141 gcgcggtggt tttaagcgcg tccgacaaac cgggcatcat ccacagcaaa ctcaaagccg
    28201 acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat cgcctgcggc aaagtttccg
    28261 gttcgccgta catttcgatg gacggtcccg gcgtgttcaa gtttgccgtc aaaatgctgt
    28321 ccaaaatcgc cgatgacgtt atcgaagaag caggttacac cgccgaacaa atcgactgga
    28381 tcgtgccgca tcaggcaaac cgccgcatta tcgaatcgac cgcgaaacat ttgggtttga
    28441 gtatggacaa agtcgtcctg accgtccaag accacggcaa cacgtccgcc gcatccattc
    28501 cgctggcttt ggacgcgggc atccgcagcg gacaaatcaa acgcggtcaa aacctgctgc
    28561 tcgaaggcat aggcggcggt ttcgcgtggg gcgcggtgct gttgcaatat tgaaaccggt
    28621 gccgtctgaa acaggctttc agacggcatt tcccatatca tgaagcggca ggctttcttc
    28681 aaaccgatgg cgtgtgcggc atttctgtcc gccgtttcgc tgcgcctccc cgtattgggc
    28741 gcgtgttacg caatattgtc cctctatgcg tttgcacttt acggcatcga caaacggcgt
    28801 gccgtgcggg gaaaacgccg cattcccgaa caccgcctgc tcctgcctgc cttgttcggc
    28861 ggttggacgg gcgcatactt gggtagtagg atgttcaggc ataaaacggc gaaaaagcgt
    28921 tttgttgtgc tgttccgtct gactgtttcg ggcaatgtcc tggcgacctg catcctgatt
    28981 gattatttcg ttccgcccga actttttgta aaactcgggc aacatctctg acaatccaac
    29041 ccaacaaagg aatactatgt ctttcgcatt cttcttcccc ggacaaggtt cccaaagcct
    29101 cggcatgatg aacggctttg ccgaacacgt catcgtcaaa aacacatttg acgaagcctc
    29161 cgccatattg gggcaggact tgtgggcgat gattaacggc agcgatgccg aaatcatcgg
    29221 tcaaacagtc aacacccagc ccattatgct cgccgccggc gttgccgttt accgcgccta
    29281 tttggaagtg ggcggcaaaa cgcctgccgc cgttgccgga cacagcctcg gcgaatacac
    29341 cgcgcttgtc gccgccgaag cattggattt tgccgacgcg gtcaaactcg tgcgcctgcg
    29401 cgccgaactg atgcagtccg ccgtaccgca aggcgtaggc gcaatggcgg ccattttggg
    29461 tttggaagat gaacaggtca ggcagatttg tgccgaatcc gcacagggcg aagtggtcga
    29521 agccgtcaac ttcaactcgc ccggacaagt cgtgattgca ggcaacgccg ccgccgtcgg
    29581 acgcaccatg gctgccgcca aagaagccgg agccaaacgc gccctgccgc tgccggtatc
    29641 cgttccttcc cattgcagcc tgatgaaacc cgccgccgac aaacttgccg aaacactcaa
    29701 aaccgttgaa atcaagcagc cgcaaatccg tgtcatccac aacgccgacg ttgccgccta
    29761 cgatgatgcc ggcaaaatca aagacgcgct cgtccgccag ctttacagcc ccgtgcgctg
    29821 gacggaaacc gtcaacgccc tcgtttcaga cggcattgcc gaatccgccg aatgcggccc
    29881 gggcaaagtg ttggcgggct tggcaaaacg catcaacaag gccgccgcgt gcagcgcact
    29941 gaccgatgcc ggacagatta ccgcctttat cgaagcgcac tgacttcgtt ccgcaaaaag
    30001 cagcctgccc tcttcaggct gcttttcacg tccgaacggc aaacagccgc atatttgcgc
    30061 tataatccat ccctaccaaa ccaccgacag cggctgccgt tgcagttccc gccctaccga
    30121 tatgatagaa aaactgactt tcggactgtt taaaaaagaa gacgcgcgca gctttatgcg
    30181 cctgatggcg tacgtccgcc cctacaaaat ccgcatcgtt gccgccctga ttgccatttt
    30241 cggcgttgcc gccaccgaaa gctacctcgc cgccttcatc gcccctctga ttaaccacgg
    30301 cttttccgca cccgccgcgc cggccgagct gtccgccgcc gccggcatca tttccaccct
    30361 gcaaaactgg cgcgaacagt ttacctatat ggtttggggg acggaaaaca aaatctggac
    30421 cgtcccgctc ttcctcatca tcctcgtcgt catccgtggc atctgccgct ttaccagcac
    30481 ctatctgatg acttgggtct ccgtgatgac catcagcaaa atccgcaaag atatgtttgc
    30541 caaaatgctg accctttcct cccgctacca tcaggaaacg ccgtccggca ccgtactgat
    30601 gaatatgctc aacctgaccg aacagtcggt cagcaacgcc agcgacatct ttaccgtcct
    30661 cacgcgcgac acgatgatcg ttaccggcct gaccatcgtc ctgctttacc tcaactggca
    30721 gctcagcctc atcgtcgtcc tgatgttccc cctgctctcc ctgctctcgc gctactaccg
    30781 cgaccgtctg aaacacgtca tttccgactc gcaaaaaagc ataggcacga tgaacaacgt
    30841 gattgccgaa acccatcagg gacaccgcgt cgtcaagctg ttcaacgggc aggcgcaggc
    30901 ggcaaaccgg ttcgacgcgg tcaaccgcac catcgtccgc ctcagcaaaa aaatcacgca
    30961 ggcaacggcg gcacattccc cgttcagcga actgattgcc tccatcgccc tcgccgtcgt
    31021 catcttcatc gccctgtggc aaagccaaaa cggctacacc accatcggcg aatttatggc
    31081 attcatcgtc gcgatgctgc aaatgtacgc ccccatcaaa agccttgcca acatcagcat
    31141 ccctatgcag acgatgttcc tcgccgccga cggcgtatgt gcatttctcg acaccccgcc
    31201 cgaacaggac aaaggcacgc tcgcgccgca gcgtgtcgaa gggcgcatca gcttccgcaa
    31261 cgtcgatgtc gaataccgtt cagacggcat caaagccctc gacaacttca acctcgacat
    31321 cagacaaggc gaacgcgtcg ccctggtcgg acgttccggc agtggcaaat ccaccgtcgt
    31381 caaccttctg ccccgctttg tcgaaccgtc tgccggcaac atctgcatag acggtatcga
    31441 catcgccgac atcaaactcg actgcctgcg cgcccaattc gccctcgtct cccaagacgt
    31501 attcctgttt gacgacaccc tgtttgaaaa cgtccgatac agccgtcccg acgcgggcga
    31561 agccgaagtc ctgtccgccc tccaagccgc caacctgcaa agcctgatcg acgcatcccc
    31621 gctcggactg caccagccca tcggatcgaa cggcagcaac ttgtccggcg gacagcggca
    31681 acgcgtcgcc atcgcccgcg ccattttgaa agacgcgccg atattattat tggacgaagc
    31741 caccagcgca ttggacaacg aatccgaacg cctcgtccaa caagcgctcg aacgcctgat
    31801 ggaaaaccgc accggcatca tcgtcgccca ccgcctgacc accgtcgaat ccgccgaccg
    31861 catcatcgtc atggatggcg gcaaaatcat cgaacaaggc acacacgatc aactcatgtt
    31921 ccaaaacggc tactacacca tgctgaggaa catctccgga aaagataccg ccgccgttca
    31981 gacggcataa acaaaatgcc gtctgaaatg gtacaatcat ccaaatccct tcagacggca
    32041 tcatatccgc cgacccatcc gattatcttc aaccaccgta aaacccatta tgacccaaga
    32101 caaaatcctc atcctcgact tcggttcgca agttacccgg ctgatcgccc gccgcgtgcg
    32161 cgaagcacac gtttactgcg agctgcattc cttcgatatg cctttggacg aaatcaaagc
    32221 cttcaacccc aaaggcatca tcctttccgg cggccctaat tccgtttacg aatccgacta
    32281 tcaagccgat accggtattt ttgatttggg cattccggtt ttgggcatct gctacggcat
    32341 gcagtttatg gcgcaccact tgggtggcga agtgcagccc ggcaaccagc gcgaattcgg
    32401 ttacgcgcaa gtcaaaacca tcgacagcgg actgacacgc ggcattcaag acgatgcgcc
    32461 aaacacgctc gacgtatgga tgagccacgg cgacaaagtg tccaaactgc ccgacggttt
    32521 cgccgtcatc ggcgacactc cgtcctgccc gattgccatg atggaaaaca ccgaaaaaca
    32581 attctacggc atccagttcc accccgaagt tacccacacc aaacaaggcc gcgccctgtt
    32641 gaaccgcttt gtctcggaca tctgcggcgc gcaaccgggc tggacgatgc ccaactacat
    32701 cgaagaagcc gttgccaaaa tccgcgaaca ggtcggcagc gacgaagtga ttttgggtct
    32761 gtcaggcggc gtggactctt ccgtagccgc cgcgctgatt caccgcgcca tcggcgacca
    32821 actgacctgc gtgttcgtcg atcacggttt gttgcgcctg aacgaaggca aaatggtgat
    32881 ggatatgttc gcccgcaact tgggtgtgaa agtgatacac gtcgatgccg aagggcagtt
    32941 tatggcgaaa ctcgccggcg tgaccgaccc cgagaaaaaa cgcaaaatca tcggcgcgga
    33001 atttatcgaa gtatttgatg ccgaagagaa aaaactcacc aacgccaaat ggttggcgca
    33061 aggcacgatt taccccgacg taatcgaatc cgcaggcgcg aaaaccaaaa aagcccacgc
    33121 catcaaatcg caccacaatg tcggcggcct gcctgaaaac atgaagctca agctgcttga
    33181 gccgttgcgc gacttgttta aagacgaagt gcgcgaactg ggcgttgctt tgggtctgcc
    33241 gcgcgaaatg gtgtaccgcc atccgttccc cggccccggt ttgggcgtgc gcatcttggg
    33301 tgaagtgaaa aaagaatacg ccgacttgct ccgtcaggcg gacgatattt tcatccaaga
    33361 attacgcaat actaccgacg aaaacggcac gtcttggtat gacctgacca gccaagcctt
    33421 cgccgtgttc ctgcccgtca aatccgtcgg cgtgatgggc gacggtcgta cttacgacta
    33481 cgtcgtcgca ctgcgcgcag tcatcaccag cgactttatg actgcacact gggcagagct
    33541 gccatactca ctgctcggcc gcgtgtccaa ccgcatcatc aacgaagtca aaggcatcaa
    33601 ccgcgtggtg tacgatgtca gcggcaaacc gcccgccacc atcgagtggg aataaacagc
    33661 aaacagggct gcaccgtccg gcgcagtcct tcgattatcg gaaaaaagga aaaaatatga
    33721 gcacacaaga tttaagcggc aaaatcgctt tggtaaccgg cgcatcgcgc ggcatcggcg
    33781 cggcaattgc cgacacactg gcggcagccg gtgccaaagt catcggtacg gcaaccggcg
    33841 agagcggtgc ggcggcgatt agcaaacgtc tggcacaatg gggcggcgaa ggccgcgtat
    33901 taaattccgc cgaacccgaa accgtcgaaa acctgattgc cgacatcgaa aaaacgttcg
    33961 gcaaactcga cattctggtc aacaacgccg gcatcacccg cgacaacctg ttgatgcgta
    34021 tgaaagaaga agagtgggac gacatcatgc aggtcaacct caaatccgtg ttccgcgctt
    34081 ctaaagccgt cttgcgcggc atgatgaagc agcgcgccgg ccgcatcatc aacatcacct
    34141 ccgtcgtcgg cgtgatgggc aatgccgggc aaaccaatta tgccgccgca aaagcgggct
    34201 tgatcggttt tgccaaatcc atggcgcgcg aagtcggcag ccggggcatt accgtcaact
    34261 gcgtcgcccc gggctttatc gataccgata tgacccgcgc cctgcccgaa gaaacccgcc
    34321 aaacatttac cgcccaaacc gccttgggca gattcggcga cgcgcaagac atcgccgatg
    34381 cggttctgtt cctcgcttcc gatcaagcga aatacatcac cggtcaaacg ctgcacgtca
    34441 acggcggtat gctgatgccc taacggacag gcttttcaac catgccgtct gaagcccttt
    34501 cagacggcat ttgcattccc aaacaaaatg aacactcacc accacctgcc ctgcccctgc
    34561 ggttcaggca caagctatgc cggctgctgc cgaccgcttc atctgcgcca aatcctgccg
    34621 cccaccgccg aagccctcat gcgttcgcgg tacggcgcgt atgtgctgca ccttatcgac
    34681 tacatcatcg ctactaccgt tccggcgcaa caaacattcc tcgatgccgc cgaactgatg
    34741 cagtggagca gagaagccga atggctgggc ttgaatgtca tcgcacaccg caacctcggc
    34801 aaacagcacg cccaagtcga atttgaagcc tacttccaag acggcggaaa ccgaacagtg
    34861 catcacgagc tgtcggcatt cgtgaaaata gcggagcaat ggtatttcat cgatccgact
    34921 gttccgctgc ctaccatgaa acagccctgc atttgcggat cgggcaaaaa attcaaagcc
    34981 tgctgcggca aatatctgca acctgtcgca taaaaatgcc gtctgaacgt tcagacggca
    35041 ttttcaacgc gcaaaaaaaa ccattcatac caagggtaag tatgaatggt caatacattg
    35101 cgggaaaaca tcttacttgc tgcactgccg aaaagggaga aacggcagcg ttaaccagca
    35161 aaagcattgt acctttatta atgttaaaaa acattaattt tgaaaatata ttaacgaata
    35221 tatctggaac ctactggttt tcccgtatcc acccgaccca gcgttcaaac agcttcggtt
    35281 cgagcgcggc gacgaccgag cgtttgaaca cgtgttcacc actccaaaac ccgtcgcctt
    35341 ccaaagtcgt cagcctgccg cccgcctcct cgaaaatcaa cgcacccgca gcataatccc
    35401 acagcttctg cccgccgtgg acataaacat cataacgccc gcacgccaga taacaccagt
    35461 ccagcgtgct gctgcccata ctgcgtatcg tcccgaaagg cgcgagcgta ctcatgcggc
    35521 tggaaagttt gcccgaacgc agatatttga tttccacgcc cgcaatcgcc tcattgagtt
    35581 ttttatccac gagacgcaaa ggcaggcgtg tcccattaag aaacgcaccc tgcccgcgtt
    35641 cggcataaaa acattcgccg ctgaccggat tgtagattac gcccaattcg gcgcgcccgt
    35701 tgcggacaaa cgccaccgac acggcaaaat gcggcagccc gttgacaaaa ttgttcgtcc
    35761 cgtctatcgg atcgacaatc cacagcccct tttcccccga atattgttcc cacaaagccg
    35821 actgttcctg ccgcgacatt tcctcgccca acatcgggca atcgatcaga agcggcaacg
    35881 cggcggcaaa agccgtctgc gcggcaatgt ccgcctcgct caacatcgaa ccgtcttcct
    35941 tgcggcggga cggcgtattc aaaaaacgcg gcataatttc ggtttgcgcg atatggcgca
    36001 cgactttctg caaacggtgt aacacttcct actgtcctca tattttgaac ttgcggcgcg
    36061 cgaacgtata atgtccgttt ccatcacgcc gctgcgacgg attataaccg tccgaaccgc
    36121 caaaaactat gcccagattc tacctgcccg aaaacctttc cgtcggacaa accgtcgccc
    36181 tgcccgacaa catcgtccgc cacctcaacg tcctgcgcgt ccgccccaac gaaaacatca
    36241 ccctcttcga cggcaaaggc aaggcacata ccgcacggct gaccgttttg gaaaaacacc
    36301 gcgccgaagc cgaaatcctg cacgaagaca caaccgacaa cgagtccccg ctcaacatca
    36361 cgctcatcca atccatttcc tccggcgatc gcatggattt caccctgcaa aaaagcgtcg
    36421 aactcggcgt aaccgccata cagcccgtca tcagcgaacg ctgcatcgtc cgcctcgacg
    36481 gagaacgcgc cgccaaacgc ctcgcacgct ggcaggaaat cgtcatctcc gcgtgcgaac
    36541 aaagcggcag gaacaccgtt ccccccgtac tgcccatcat cggctaccgt gaagcactcg
    36601 acaaaatgcc gtctgaaagc accaggctga ttatgagcat caaccgcgcc tgcaaactcg
    36661 gcgacatccg ccacccgtcc ggcgcaatcg tctttatggt cgggcccgaa ggcggctgga
    36721 cagaacagga agaacaacag gcatttgaag ccggctttca ggcggttaca ctcggaaaac
    36781 ggattttacg cacagaaacc gccccactcg ccgccatcgc cgcgatgcag acgctttggg
    36841 gcgattttac ctagcagatg aaaaaacacg ggattttatc cgaaaaagca acaatccccc
    36901 cgccgtcatt cccgcgaaag cgggaatctg gaaacgaaaa actacaggga tttatccgaa
    36961 acaacaaacc ctctccgccg tcattcccgc gcaggcggga atctagaaat ttaacgttgc
    37021 ggtgatttat cggaaatgac tgaaactcaa cgaaccggat tcccactttc gcgggaatga
    37081 cgaagtggaa gttacccgaa acttaaaaca agcgaaaccg aacggactgg attcccgcct
    37141 gcgcgggaat gacgaatttc aggtttctgt tttcaggttt ctgtttttgg ttttctgttt
    37201 tcgcgggaat aacggaattt taagttttag gaatttgtcg gaaaaacaga aatccccccg
    37261 ccgtcattcc cgcgaaagcg ggaatctaga attttaatgc ggcaagaatt tatcggaaaa
    37321 aaccgaagtt taaagaccta gattcccgcc tgcgcgggaa tgacagtgtg tccatttctg
    37381 attttaatcc cctatatttt acacaaacta tttgaacaat atgacccgcc tgccgtaaac
    37441 tttctcaagc tccgcctgcc tttgacgctc cattcttttc ttcttttccc taccgaattt
    37501 acccaaagca cgcttcaagt caaacatcac cttcaacgaa cggcggtgtc ttcttccttg
    37561 ttccctatct ttttccaaat cgctacccaa catactgttt tgactgagaa acttggcata
    37621 atgcaattct tgggtacata aggcgggact aacctgataa acaggcatcc cctccttatc
    37681 aaagaaataa gtaaacatca tcaaatctac cgctttaatc cgctctgccg gcaaaacggc
    37741 aaacctgtcc aagaaaaa
//
LOCUS       DAXFHF010000015        37582 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXFHF010000015 DAXFHF010000000
VERSION     DAXFHF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37582
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.015"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..998
                     /locus_tag="ACFIRM_000645"
     CDS             <1..998
                     /locus_tag="ACFIRM_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC3028324.1"
                     /translation="QAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIHHFDEIYEK
                     LTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTVYYSRTAKK
                     PNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKNSGGNIQVI
                     YPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKDEIQSDLYQ
                     IDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRATGT
                     MLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK"
     gene            complement(1061..2125)
                     /gene="hemE"
                     /locus_tag="ACFIRM_000646"
     CDS             complement(1061..2125)
                     /gene="hemE"
                     /locus_tag="ACFIRM_000646"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC3028325.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFAHA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMVQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(2200..3417)
                     /locus_tag="ACFIRM_000647"
     CDS             complement(2200..3417)
                     /locus_tag="ACFIRM_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC3028326.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(3414..4748)
                     /locus_tag="ACFIRM_000648"
     CDS             complement(3414..4748)
                     /locus_tag="ACFIRM_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC3028327.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(4760..5524)
                     /locus_tag="ACFIRM_000649"
     CDS             complement(4760..5524)
                     /locus_tag="ACFIRM_000649"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC3028328.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPTAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(5542..5964)
                     /locus_tag="ACFIRM_000650"
     CDS             complement(5542..5964)
                     /locus_tag="ACFIRM_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC3028329.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(6246..7214)
                     /locus_tag="ACFIRM_000651"
     CDS             complement(6246..7214)
                     /locus_tag="ACFIRM_000651"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3028330.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVNEPKLKESLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(7214..8038)
                     /locus_tag="ACFIRM_000652"
     CDS             complement(7214..8038)
                     /locus_tag="ACFIRM_000652"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC3028331.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(8068..8709)
                     /locus_tag="ACFIRM_000653"
                     /pseudo
     CDS             complement(8068..8709)
                     /locus_tag="ACFIRM_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(8878..9198)
                     /locus_tag="ACFIRM_000654"
     CDS             complement(8878..9198)
                     /locus_tag="ACFIRM_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC3028332.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDFLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(9221..9847)
                     /gene="upp"
                     /locus_tag="ACFIRM_000655"
     CDS             complement(9221..9847)
                     /gene="upp"
                     /locus_tag="ACFIRM_000655"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC3028333.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFADSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(9953..10264)
                     /gene="grxD"
                     /locus_tag="ACFIRM_000656"
     CDS             complement(9953..10264)
                     /gene="grxD"
                     /locus_tag="ACFIRM_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC3028334.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(10365..10580)
                     /locus_tag="ACFIRM_000657"
     CDS             complement(10365..10580)
                     /locus_tag="ACFIRM_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC3028335.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(10606..11385)
                     /locus_tag="ACFIRM_000658"
     CDS             complement(10606..11385)
                     /locus_tag="ACFIRM_000658"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3028336.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(11390..11740)
                     /locus_tag="ACFIRM_000659"
     CDS             complement(11390..11740)
                     /locus_tag="ACFIRM_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC3028337.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(11744..12721)
                     /locus_tag="ACFIRM_000660"
     CDS             complement(11744..12721)
                     /locus_tag="ACFIRM_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC3028338.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(12758..13885)
                     /locus_tag="ACFIRM_000661"
                     /pseudo
     CDS             complement(12758..13885)
                     /locus_tag="ACFIRM_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            14053..14754
                     /locus_tag="ACFIRM_000662"
     CDS             14053..14754
                     /locus_tag="ACFIRM_000662"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC3028339.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            14896..16689
                     /gene="lepA"
                     /locus_tag="ACFIRM_000663"
     CDS             14896..16689
                     /gene="lepA"
                     /locus_tag="ACFIRM_000663"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC3028340.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLNLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            16835..17854
                     /gene="lepB"
                     /locus_tag="ACFIRM_000664"
     CDS             16835..17854
                     /gene="lepB"
                     /locus_tag="ACFIRM_000664"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC3028341.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRAPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(17959..18066)
                     /locus_tag="ACFIRM_000665"
                     /pseudo
     CDS             complement(17959..18066)
                     /locus_tag="ACFIRM_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18290..18439)
                     /locus_tag="ACFIRM_000666"
     CDS             complement(18290..18439)
                     /locus_tag="ACFIRM_000666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028342.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(18485..19392)
                     /locus_tag="ACFIRM_000667"
                     /pseudo
     CDS             complement(18485..19392)
                     /locus_tag="ACFIRM_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(19701..20633)
                     /gene="cysK"
                     /locus_tag="ACFIRM_000668"
     CDS             complement(19701..20633)
                     /gene="cysK"
                     /locus_tag="ACFIRM_000668"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC3028343.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(20829..21434)
                     /locus_tag="ACFIRM_000669"
     CDS             complement(20829..21434)
                     /locus_tag="ACFIRM_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028344.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(21431..22282)
                     /gene="dapF"
                     /locus_tag="ACFIRM_000670"
     CDS             complement(21431..22282)
                     /gene="dapF"
                     /locus_tag="ACFIRM_000670"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC3028345.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            22419..22733
                     /locus_tag="ACFIRM_000671"
     CDS             22419..22733
                     /locus_tag="ACFIRM_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028346.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            22730..23398
                     /locus_tag="ACFIRM_000672"
     CDS             22730..23398
                     /locus_tag="ACFIRM_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3028347.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(23471..25594)
                     /gene="pnp"
                     /locus_tag="ACFIRM_000673"
     CDS             complement(23471..25594)
                     /gene="pnp"
                     /locus_tag="ACFIRM_000673"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC3028348.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRRIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKRLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(25828..26691)
                     /locus_tag="ACFIRM_000674"
     CDS             complement(25828..26691)
                     /locus_tag="ACFIRM_000674"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC3028349.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPV
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            26884..27747
                     /gene="rfbD"
                     /locus_tag="ACFIRM_000675"
     CDS             26884..27747
                     /gene="rfbD"
                     /locus_tag="ACFIRM_000675"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC3028350.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(28118..28351)
                     /locus_tag="ACFIRM_000676"
     CDS             complement(28118..28351)
                     /locus_tag="ACFIRM_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3028351.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(28369..28503)
                     /locus_tag="ACFIRM_000677"
     CDS             complement(28369..28503)
                     /locus_tag="ACFIRM_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028352.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            complement(28517..28717)
                     /locus_tag="ACFIRM_000678"
     CDS             complement(28517..28717)
                     /locus_tag="ACFIRM_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC3028353.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(28889..29764)
                     /gene="xerD"
                     /locus_tag="ACFIRM_000679"
     CDS             complement(28889..29764)
                     /gene="xerD"
                     /locus_tag="ACFIRM_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC3028354.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(29816..30256)
                     /locus_tag="ACFIRM_000680"
     CDS             complement(29816..30256)
                     /locus_tag="ACFIRM_000680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC3028355.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            30577..31515
                     /locus_tag="ACFIRM_000681"
     CDS             30577..31515
                     /locus_tag="ACFIRM_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC3028356.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            31644..31937
                     /gene="hfq"
                     /locus_tag="ACFIRM_000682"
     CDS             31644..31937
                     /gene="hfq"
                     /locus_tag="ACFIRM_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC3028357.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            32022..32588
                     /locus_tag="ACFIRM_000683"
                     /pseudo
     CDS             32022..32588
                     /locus_tag="ACFIRM_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            32642..33016
                     /locus_tag="ACFIRM_000684"
     CDS             32642..33016
                     /locus_tag="ACFIRM_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC3028358.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            33026..33520
                     /gene="folK"
                     /locus_tag="ACFIRM_000685"
     CDS             33026..33520
                     /gene="folK"
                     /locus_tag="ACFIRM_000685"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC3028359.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(33648..34127)
                     /locus_tag="ACFIRM_000686"
     CDS             complement(33648..34127)
                     /locus_tag="ACFIRM_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC3028360.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(34347..35084)
                     /gene="ubiE"
                     /locus_tag="ACFIRM_000687"
     CDS             complement(34347..35084)
                     /gene="ubiE"
                     /locus_tag="ACFIRM_000687"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC3028361.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(35127..35495)
                     /locus_tag="ACFIRM_000688"
     CDS             complement(35127..35495)
                     /locus_tag="ACFIRM_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC3028362.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            35615..37234
                     /gene="mnmC"
                     /locus_tag="ACFIRM_000689"
     CDS             35615..37234
                     /gene="mnmC"
                     /locus_tag="ACFIRM_000689"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC3028363.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
BASE COUNT         9341 a        10400 c         8695 g         9146 t
ORIGIN      
        1 tccaagcctt tttaaatgac ttagtgcaag cagtcaataa tggggaagac cttgcaggta
       61 gctttaaaga aagttatttg gatttgcaaa agaccaagcc cgatattcat cactttgatg
      121 aaatatacga aaaattgaca gcacttttag agaataaaca aatctctact cttgtcgtta
      181 actcgcagac agaaacagat tttgacttag aaaaaggttt caacatcatt attggtggta
      241 atgtgattgg tcgtggcttg actattccga aactacaaac ggtttattat agtcgcactg
      301 ccaaaaaacc gaatgcagat actttctggc aacattcgcg tatttttgga tatgaccgcg
      361 ataaatcatt attacggctt tatattccgt tcgatgtcta ttacttcttt gtacaactca
      421 atcaagcaaa taatctgatt attgggcagg caaaaaattc aggcggcaat attcaggtta
      481 tttatccgaa aaatatcaat ccaactcgaa aaaatgtatt gaaattcgat agtatcaatc
      541 aaattgtggg tggtgtgaat tattttccac tccacccaaa cgaagataat ttatcggaga
      601 ttaacaaaat tttgccttct attttgaaag atgaaattca atctgattta tatcaaatag
      661 atatagaaga tttattcttg gttctagata aattgggtcg ttatgttcca gatgactgga
      721 ataaggaaaa atttattgct ggtgtagaag cattaaaagc acaacgtcca agttttaaaa
      781 cctatgtttt gatcaaaacc ggacgtaaac tttctcgagc aaccggcaca atgctttcgg
      841 aagacgaccg taaattagga gaaaaatatc ccaatgattt attccttacg ctttatcaag
      901 tagtaggaaa taaagacaaa ggttggcagg gaaaagattt ttggttaccc aatatcaagc
      961 taccacacaa tggcttggtg taccaaagtg caaaatgaag catatgccgt ctgaaaactt
     1021 ccgattcttc agacggcata tcgggcggtt tcctgccggc ttacccgccg tgatactgcc
     1081 gcgacagctc gtgtacggtg tcgactaaga ttttggcgtg ttcggggtcg gcgtgttggt
     1141 tgatgccgtg tccgaggttg aagacatggc cgctgccgtg tccgtagccg gtcaggatac
     1201 gtgcgacctc ggtgcggatg gattcgggcg taccgaagag ggcggacggg tcgaagttgc
     1261 cttgcagggc gacttggttg ccgacgcggc ggcgtgcttc gccgatgttg cacgtccagt
     1321 ccaagcccaa tgcgtctgcg ccgatttgga ccatgctttc cagccacagc ccgccgcctt
     1381 tggcaaacac gataacaggc acgcggcgac cttcgctttc acgtttgagt ccggcaacga
     1441 tttggcggat gtatttgagg ctgaactctt taaacgccgc gtcgctcaac acgccgcccc
     1501 aagtgtcgaa aatctgcacc gcctgcgcgc ccgcgtcgat ttgggcgttg aggtaggcgg
     1561 taacggcttg ggcgttggta tcgaggattt tgtacagcaa atcggggcgc gagtacatca
     1621 tggttttgat ggtgcggaat tctttgctgc cgccgccttc gaccatataa caggcgagcg
     1681 tgaacgggct gccggagaag ccgataagcg gtacgcggcc gtccaatgct ttacggatgg
     1741 aagttaccgc gtcgaaaacg tattgcagtt tttccatgtc gggaacgtgc agcttggcga
     1801 tgtcggattc gtgttgcaag gcgtgtgcga atttcgggcc ttcgccttcg gcaaaataca
     1861 gccccaagcc cattgcatcg ggaacggtca agatgtcgga aaacaaaatc gccgcgtcca
     1921 aatcgaagcg ttccaaaggc tggatggtga cttcggtcgc caatccggtg tttttgcaca
     1981 aatcgaggaa gctgcccgct ttcgcgcgtg tggctttgta ttcgggcaga taacgccccg
     2041 cctggcgcat catccaaatc ggtgtgtatt cgacgggctg tttgagcagg gcgcggagga
     2101 aggtatcgtt ttttaaaaga gtcatacggg ttttctgtca gtctgtttgt caagtgaaat
     2161 ggttttcaga cggcatttca acgatgccgt ctgaaggttt caacgggttt cggcggaagg
     2221 gcggttttcc ccggcaacgc ttgccaaaac caagttgcgc tgtgcttcgg ctttttgcga
     2281 ctgtgcggtt tcgtcaaaaa cctttgccaa caccaaacgc gccggaatac tcggcttcag
     2341 tgcaatactc gcttcaaggt agccttttgc cttaccccaa agtttgcggc cgtaggcgag
     2401 ccggccgaga tacatcagca gaagcgcgtt atcgggctgt tctttcagcc aagaatcggc
     2461 aaaatcgatg gctttctgct gttcgcgctc gcccaaaaag cgcacgcttt cgacaaaggc
     2521 ttccaaaagc tcggggcggc ggttgtgcgg ataatgctgt ttgacccatt tgaccgcatc
     2581 ggcatacagt cccaaacgtt cgtacttttc cgcaaccgat acgctcaatt ccccgttttt
     2641 gaggctgtcg ggaatccgct tcaggcaggt tttcaaagcg gcggcatcgg cagcatccgc
     2701 catctggcgg cggtatgccc aattttgata ccgttccatt tccgatttgc ccaacgcgcc
     2761 cgccttggaa agtttttcgg tttttgccag aacctgcaac gcatcgcccc gatcgaaggc
     2821 gtaacgaagt tgcagacgca cgaggcgcgt aaggttggca ttcatcttcg ccgccgcatg
     2881 aagattggct tccgccgctt cgtaatcgcg ccggtttaac gccgattccg ccagcagaag
     2941 atagcgggaa agctgctgtt tttccggcag tttggcgatt tccgcaagat aacggtcgcg
     3001 cagctcgata ttttccatct gtcctgccgc gtgcgcgccc agcatcaatg ccaaagtccg
     3061 gttgtctccg gcctctttgt tgcccaacac tcgagaggct tcgagttccg ccttttcaaa
     3121 acgcccttcg aaatacgcca aacccgcctt attcaaggca agcgcggcct tgcggccttt
     3181 ccgcgccgaa ccggaacgct gcatcttttc ggggatatta agtacgccga tgatgaattt
     3241 aaacaggaaa taccacacca cgacggcaat cagcgaacct aacacaaaag cgtgcaggtt
     3301 gattctgagc atggtctgtc cgagtacgat atacacgtcg ccggtgtaaa tgcccgaagc
     3361 cagcgccagt ccgacggcgg cggcaaacag gacaacaatc cagactaccg ttttcatgcg
     3421 cgttctcctt tagcggacgg cgaacgccct gccttttccg gtgcgggctg tttttttgca
     3481 ggctgttccg gtttgcgttc cgaaggcagg gacggtgctt ctatggcctt cacatccgat
     3541 gcggcagccg gttcggaagc tgtgttttcg tttgccggtt cggaagccgc ctgttccgct
     3601 tcttgatttt ccgccgccgt catgcgcgta ccgtcgcgat aggcgcggac ggcatttaaa
     3661 ctggtcttca agccgtcatc cgccgtcatg cgtacatcca gtgttttcaa ttccgccagt
     3721 tctttcagcc acgactgcgt ggcgggcgac ttggcatcga aatactgtct gacggcggct
     3781 tcggcattgt tcaaatcgcc ctgatagact tcgccgttgc gctgcatcaa tgcggtgcgc
     3841 gcatccaaca gacgcagacg caggttttca cgcacaaaat atgcctgttc gggagaaatc
     3901 agcatggcat cgttgttttc caaacggcgg atttcgacca gccccttcaa tgtgccgagg
     3961 gatttttccc atacgttctg ccaccatgaa gcggaaacgg cttcgttgcg ggcctgtacg
     4021 cccggtttca atacgccgtc caaaatcagc ggcagtccgg atacggcggt ttccaaccgg
     4081 tcgaggcgca atgccgtgcc ggaaatatcg acatagggac ggtttttcag ttccgccaaa
     4141 tcgctgctga ccgcctgttt gatcggcaga agctctgcct gatcgaaacg ggacaggcgg
     4201 ctgtcgatat gctccaacac gccgactgcc gtttggatat tgccggtcaa caccagctgt
     4261 tgcgccgcca gattgagtat ggtctcggtt tcgtccacca gccaatcggc acgccctttg
     4321 gtcagctcgc gataggcttt ttgcgtcatc aggatttgtt cgccgttggc tttgacaccg
     4381 ctgtccaaac ggtcgagttc ggactgtatg gcactttggc ggttgaggtt gtctttgagc
     4441 agggcggcgt tttccgactc gcccaaggcg gctttgtcga ttttctggtt gaatgccagc
     4501 tcttggtttt tcaagacatt ctgtccttgg acaaacaaaa aaccacttgc acccaaaccc
     4561 aatgccgcca ataccaaggc gcaggccgcc aaagcgttgc tgccggactg tttgacgata
     4621 aaaggagcgg cgggaatgtg tacttctgtt tcgttttctt tgcgtgggga agaggtttca
     4681 gacggcattt cttttgatgc ctgtatctcg cgtacgggtt cggatgattt gttttcaggt
     4741 tcgcccaccg ttttgctcct tattggtttg aggttccggg aaagaccatg ccgtctgaaa
     4801 cggaaatgga agaataggaa agcgcggctt ccagcgtagg gacggtttcg accgaacaca
     4861 cgccttcgcg cttcaatgcc tccgcaatgc gcggatgatg ggtaaagtat agcaaggatt
     4921 tgaagaatcg ggaaaattgc ggcggaagct gtgcaaacag cgactgcaca agttctgtgg
     4981 acgtaatata ggcggcggca atattttcgg tttggaaatt ttgaaagttc aaaggtttat
     5041 gccgtctgaa atagacttct gccacctccg tccgaaaacc tttctcctgc aaggcattca
     5101 tcagaaaatc ccgcccgccg tgtccgcgca cagacaatac gcgcgcacct tcgggcagac
     5161 tgttccaaac cggcaggcgc aaaaccgcct cgctgtcgtt gccgtcttca ggtgcaaaca
     5221 cgcccgcgcc cgcataacgc gccaaggcac ggcggctgcc ttgccctacg gcaatgtgcg
     5281 cctttatgcc gtctgaaagg ttaaggtacg ggacggcggt ttcaacggcg gtcggactga
     5341 cccaaaacac ggcatccgca cgggcataca tttccggcag ccgtttcagg cttgcttcgt
     5401 cggtttcgat ttcaatcgga ctcaacactt ccgcctgcca tccggcacga cggcagactt
     5461 ccacatcgtc cttcgccctg cccgacgggc ggacaatcag catgacgggc ttcgctgttt
     5521 ccatcgcctt atccgccccg attaccggga aaaatcgtca tcgttgaaat tcgcctgcgc
     5581 ggttttcggc gtacagccta cccgtgcgac ttccttgccc ctacgctcca caacgacacc
     5641 gctgctttcc gtatgagtca cggaatcgaa gcctgtccat acggtgtaca taaattcgcc
     5701 gttttggaat ttcatcgttg cccggcgtcc gctgcccatg ccttgccacc tgtcggaacg
     5761 ccccaacagg tcagctttgc tgttgcgtat ggcaatttct ttttttgccg cactgccgaa
     5821 cgaatattcg taaagattgc ggttgatttt ttgaacttct atgtatttgt tgttgtccgt
     5881 tttacaggaa aataccgtat ccgcccacac ggtttgggcg gcagccgaca gaagcaatgc
     5941 ggcagaaagg aacatttttt tcatcataat ttccttaatg gttgaaaccc cgccattcgg
     6001 acatccgtcc ttcgtggcag cagcatcaga ttttatttga aaggggtgta acccctttca
     6061 aattagggca acacataggg cggcgcttta tgtgtcgtcc tgtgtgttga aacatcattg
     6121 cccgtatcct gaaatagaag gctgtgtttt aacaatccgt tgattgctta agatgaattc
     6181 acactaaaaa acagttctct atttcatcta ttacggcggg ttgttttgtt tataagggtg
     6241 gtaatctaca aattgttact aaatagcaag gtgtgaatga aacggcagat ataatcaaaa
     6301 tcttcttgtt ggtcaaaata cggtaacata atgcttttca atttttcgcc tttgggttta
     6361 cgtccataag aaaacagata gcggtttgct gtaatacatt tagcgtaata cagttttact
     6421 tctattggca tatcggactg tatgggtgtt aatacggcga catcactatt tgctacaaat
     6481 tcacaagttg aaacatagct ataagtatgg ctaccttcgc cattggtaga agtgaaaagc
     6541 gtatggcatg gaaaaatatg tttttcatca acgctatcac gagcgatata acttcgtagc
     6601 gttcttgctt gcgtactggc aggacggata tagacaaccg tgtctttttg ccgttgttca
     6661 aattctttta atttggttgc ggcaatgccg ttttgaatag taaaaatttc atcaagtcgt
     6721 tttgattgtc gaaccacagg caaatcaagc gattctttta atttcggttc gtttacaccc
     6781 gcagatggat ttaaattcac gctttcaacc cagcttggga tctcatccaa agatggcaca
     6841 aacagttgtc gtaacgtgcg gttagcttcc ctaccgcaag cagaaaaacg gtaggcattg
     6901 gcaaaaatag cagcacaata aaagagtttt tgttgttctg tcatttctgt tttgggattt
     6961 aaaacagcaa cattttgagc tgtataaaat ggtttgtttt gtaaaaaagt agataaaaca
     7021 gaaccaccta atgcaacagt taataatcct gctggataag gttttgtatt tttcaataaa
     7081 tccacagatg ctgttacacc attatttttg cctgacctgc ctacaaagtt gattgttgga
     7141 ttgaagctgc tcattttatt caaatctaat tttgaaccgt aactcacatc aaaaatctct
     7201 tgcaattcaa ccattattca tcgtctccga agtcgttatt gtcatcgtct tcatagcttt
     7261 cattatccaa attttcagtc ggccctactt ccgctcccag tagttgaaat aaagcaaatt
     7321 cacgcacgac ttgctcaaaa tctgctcggg taattttgga ataatcagtt tccaaatagg
     7381 cttcggcaac ccattcatcg ttatcagtta caaatgctgt taccgattct cctgtgtgaa
     7441 cttcgttgtt taaataatgt tccaaccagc gcgctttaat gccctgccag cggtcgtata
     7501 aatcaatgcg tcccaagtgt ttggtttttt caaaaccgtc atcacgccag tagccaaacc
     7561 acgttttgcg acaggctttt ttcgtgctga tttcttcttg tgtgtccggg tcaatcacga
     7621 ctgtctgaaa atgcggttta tgggcttcaa atacgacaat gcacgttacc gtgccaactg
     7681 ggtaaaacag ttcgctcggc atagacatca cggcttttaa gcgatgatat ttcacaattt
     7741 cgcttttggc tttgcttggt gcaatcacgc aactgactgg aataatggca atgcctgtgc
     7801 caccttctgc caacatatcc aacatttctt tgacaaaata aagttcatgc aattcggcat
     7861 cgcttttgga ttgggcgtaa ggtggattga gaaagccaac atttgggcgt tttaagcctg
     7921 tttcaggttt gggatttttg attaaatcct gaaaatcagt catgaaacaa ctgctttggt
     7981 gtaaattggc tttaccgtct ccgcgtaaaa tcatattact ggcggcaaga gcaaacattt
     8041 ttgggttgtt ttcaatgcca atcagttgat attttttaga gatggcttac ccaaattgct
     8101 aattctagca acagatttac cgtctaaatt gataccacaa ggaacacaaa cttgttcttt
     8161 ggaaatgcca cgtttcgttg ctctcgttcc tcgtttgtga gcaggacgtg gcagattaaa
     8221 atttttgtgg tgccctttgt aagaaatcgt cgaataagtt tcatcagctt gcacaatgcc
     8281 gtctaactca acttcattca tcatgttttg aagtgcgtcc aaaattttgt gccgccaagt
     8341 aaacgcagta gcaagattga ttttgcaaat ttcagcacat ttgcgaagtg ggtatttttc
     8401 aatcatacaa tgaatgtatt tttcccaaac ttcaatgtct ttttgcgtgt tgtagagaat
     8461 agtgcctgtc tgctcaacaa aagacttttt acaatcacga cacaaaaaac gttggttgcc
     8521 acaatctttg ccatttttga cgaaatgtgt agattggcaa tgtgggcact tcgtaacttt
     8581 tcttggttca atgacttttt taacctgttc ttttgagctg acagaagtca aaaaagcctg
     8641 tttatccgta tcggacaaat tttcaaaaag ttgttttaaa atgtctaatt cttttgttac
     8701 gcccgacatt ataaaatccc ttatagttca atttgttata agggatttta tcaaaaatta
     8761 gtgcaaaaat caacggattg ttaaaacaca gccaaataga aaagaaaatg cccgttttac
     8821 ggtcttcttt ccgacaaaat gccgtctgaa acgaatttca gacggcatgg tcgaaaatta
     8881 ttgcgcggca agcggtttga gcaggagggt ttcgccgctt tgcacgacat tttccagaaa
     8941 atcgatgttc ggatgcttcc ccggatgggc gcgcgcgtcg gcaacatgtt cggcaaacca
     9001 tatcaaaccc tgttctgccg cttctttacc caattttcca tcaaaacgtt ccgccaaggc
     9061 attgtacagc ttgagcgaac cgagcttgcc ctgaacggcg ggaatgtggt gaaccgtttt
     9121 gccttcggca tcctgaacat ccaaaccgcc cagatggtcg attgaaggaa atgtggcgag
     9181 atagtcttgg aaattcatat caaatccgaa aatcagtcag ttagcgcgtg ccgaaaatct
     9241 tgtcgcccgc atcgcccaga ccgggaataa tatagccgtt ttcgttcaaa cggctatcca
     9301 gcgcggcggt ataaatcgta acatcgggat gcgcatcatt aacagccttc acgccctcag
     9361 gggcggcaac caagactaag gctttgatgt tgcggcaacc tttttctttc aacaggtcga
     9421 tggtggccac catcgaaccg cctgtcgcca gcataggatc gataatcaaa gccggacgct
     9481 cgtccatact gtccgcaaat ttctcaaaat aggaaatagg cttcagcgtt tcctcgtcgc
     9541 gctgcaaacc gaccacgctg attttggcag tcggaatcag gtcgagcacg ccgtcgagca
     9601 tacccaaacc ggcgcgcagg atggggacga cggtcagggt tttgcctttg atgcggtcgc
     9661 cttcaatctg tccgcaccat ccgtcgataa ggtatttttc aatttcaaaa tcacggcttg
     9721 cctcgtatgc catcaggcgc gccaactcgg tggtcagcgt acggaatttg taggtgctgc
     9781 aatccgcctc cctcatcagg gtcagtttgt ggcggacgag cggatggttg ataacgttaa
     9841 cgttcatcgc ggtgttccag tgtgattttg agacgcgtca ttatactttt ttttaatttg
     9901 ccggaaaaga aaatgccgtc tgaaacacgt tcagacggca ttgccgaatc catcaggctt
     9961 tcagcagctc ttgcagctcg cctgcctcat acatttccat caggatgtcc gaaccgccga
    10021 caaactcgcc gttcacataa agttggggga tggtcggcca gtcgctgtat tccttaatgc
    10081 cttggcgtac ttcgggattt tccaatacgt tgacagcaac gtaatcggtg cagcctgccg
    10141 cgttcaggat ttgcacggcg cgggaagaga aaccgcattg cggaaactgc ttcgtacctt
    10201 tcataaacaa tacgacgcgg tgtgtcgtta ctacttcttt aatttggtcg tggatggaag
    10261 ccatcgctta ttccttttga taaagttgac aaaatttgtc aggtagggcg cattatacgc
    10321 aaaagcggaa acttcccaca atatttgccg tatatcgcgc agtatcaatg ttgcgaacgg
    10381 caatattccc actcctcgac aatcctgctg tccggcaaat ggcacagggc atatccgccg
    10441 ccgctttcgt ctttttttac ttcccgtctg ccgcctttgg caatgcagaa tttggaagcg
    10501 gaatttgcca tgcctgtccg aaacgtgttg gcaggcagcc ggcagcagcg cggacatgca
    10561 tacggatgaa ttgccttcat ggttttcctt atcgtcggca cagggtcaga cgacccgcat
    10621 atcgggtact tttttaaaca gccggtaaaa gttttccgtc gtatatgccg caacctgttc
    10681 caatgtttga ttccgcaatt tggcaatatg ttcggcagta tgacgcacaa aagccggttc
    10741 gttctgcctg cctcgtttgg gaacgggggc aaggaacggc gcatcggttt ccactaaaat
    10801 gcggtcgtcc ggaacgtatt tcgccgcctc ctgaaccaag ggggcgtttt taaaggtaac
    10861 gattcccgag aaagaaatat aaagccccaa atccatggca gcccgggcaa aaccgacatc
    10921 ctcggaaaaa cagtgaatca cacccgaatt ggttttacat tcttttaaaa tcgccaaggt
    10981 gtccgccgcc gcatcgcgcg tatggacgat aacgggcagt ccggtttgat tggctgcttc
    11041 gatgtggtct gcaaagcgtt tgtgctgcca agacaaatcg cctttgcacc agtaataatc
    11101 caaacccgtc tcgccgatgc cgaccacttt cggatgggcg gcggcttcga ccatttccgc
    11161 aatggaaaat tcttcggctt ccttgctgtc gggatgtacg cctatggtgc aatagatgtg
    11221 ttcgtgcgct tcggcgacgg caaagacttc ggagaagctt tccctactga cgctgatggc
    11281 gagtgcctgc cccacgccgt ttgcttccat gttggacaaa acttcgggca ggcgttcttt
    11341 caaaccttca aaattgaggt ggcagtgcga atcgatgata tgcatagcgt tacatggtaa
    11401 acgtaggtct gctgccgctg attgtgctgc ccagttcgac ttcgatctgg tctttgactt
    11461 tcaggcagtt ttcgtgtttt gtgaatgcca gccccacccc tttgggtttg gaggaagtac
    11521 tcgcaggatt aatccaggcg accttggtcg gcaggaacag tttgggaaag ttgaggattt
    11581 ctacggcaag cagaatatcg tccccgatgg aaaatacgtc gtcggtctgc acaaacagac
    11641 cgccgtgttc caaaaacggc atataggagc tgtacagcag attcatgtct ttcagctgca
    11701 acgacatcat ttttgccgga atattttgtc cgtctgacat aattcacctg ttctctttct
    11761 tcaattccaa atagttgatg agcagatgct cgatctgcat tttgacattt aaagtatgaa
    11821 acccgtaagg ggcaagctgt ttgagcatat cctccgccgc aaatacattg cgcggacgga
    11881 aacccgatgc cgtctgaagc agcctgtctt cataagcggg ataatagacg ggtttcatgt
    11941 gttgcaggca caagcctaaa tcgaccagcc atttctgcat ccacccgaca aatacggcga
    12001 gcggaagttt ttccttatcg aaaagcgcgg cgtaatccaa aatcttcaac aatctcggct
    12061 ctgccaatat ctccagcagc tttgccctca attcccgaag ctcgccttcc tcctgaaaca
    12121 gcggcgcacc cgaatggaaa gccagacgtt cctcaggctc cgccacaccc ctgtcgcgca
    12181 gatacgccaa tgcttcccca tgcgaaggag cgggcaaaac catcttccgg cagcggcttt
    12241 taatggtcgg taaaaccttg tccgccgcgt ggcttaccag caaaaagacc acttgtggcg
    12301 gcggctcttc caacactttc aacaaactgt tggcggcttg gacattcata ctttccgcag
    12361 gatgaatcag gatcacgcgc aaaccgcccc gtaccgaagt caggtacaca ttatcgatga
    12421 tttccctgac ggcatcgatt ttgatccgca acagtttgcg tccgttttcg ggttcgtccg
    12481 ccagaggcgt gatttcgtaa aaatcgggat ggcttccccg tccaaacaga tggcaggaca
    12541 tacattcgcc acagggtttg cagcccggtg caggggtttc gcacaacagc gccttcgccg
    12601 caaagcgggc aaacgcagtc ttccccgtcc cctttttgcc aacaaacagc cacgcattag
    12661 gacggctcgt ccaatgctcg gcaatctgcc gccattgttc ctgatgccac ggataaatca
    12721 tgtcaaactg cccgtccgga aatccatacc gccatcggag cagttccaaa tcggggtcgg
    12781 aactttgtgc cctgcggctg cgcaactgta ccgccaaacg caaatcatgt gcggaatcgg
    12841 catttttcaa ggcatcctgc aaggaaatct cgcctttttc atacaattgg taaaggtgtt
    12901 ggtcgaaggt ctgcataccc agggtagtgg attttttcat cacttctttg atttcatgga
    12961 tgttgccgtt gtgaatcaac tccgaaatca ggggcgaatt gagcagtacc tcgactgccg
    13021 ccaccctgcc cttgccgccg tctcgcggaa cgaggcgttg cgaaataaac gcctgaaggt
    13081 tgagcgacaa atccgtcagc aattgttcgc gccgctcctc ggggaagaag ttgatgatgc
    13141 ggtcgagcgc ctgattggtg ctgttggcgt gcagcgtcgc catacacaaa tgccccgttt
    13201 cggcaaaggc gatggcgtag tccattgttt cacggtcgcg gatttcgccg ataaggatca
    13261 catccggcgc ctgacgcagc gtatttttca acgccgccat ccagttttcc gtgtccacgc
    13321 cgacctcgcg ctgggtaatg atgcagtttt tgtgttcgtg gacaaactcg atcggatctt
    13381 cgatggtgat gatgtgtccg aacgaatttt cattgcggta gtcgataagc gaggcgagcg
    13441 aagtcgattt gcccgagccg gtgccgccga caaaaataac cagcccgcgt tttttcagcg
    13501 caacatcctt caaggccggc ggcaggttca ggctttcaaa cttgggaatc ttgctggtaa
    13561 tcgcgcggaa taccaacgcc gtcgcaccgc gctgtatcat cgcattgacg cggaagcggc
    13621 tggtgtccgg caggctgatg gcgaagttgc actcgttggt cgatgaaaat tcttccgcct
    13681 gcttcgcact cataatcgaa aaggcgattt ccatacattt ttccgccgtc agcggttcgt
    13741 ccgtgatgcg ggtgattttg ccgtccagct tcatagcggg cgggaaatgg gtcgtcacaa
    13801 acaggtcgga acctttgttt ttgttcatat ggcgcagcca tgcgaacagt tcttcctttg
    13861 cagtcatatt ttcatcggta aacatcctcg tcccccaatc ttcaaacaat ctgtaccttc
    13921 ccattctacc aaaaatccgc cgtgctgtct gaaaccgttt cagacggcac ggacaaaccg
    13981 cctccactca atcctgccgt gttagcgtgt ataattcggc gttctatccg aaattcacag
    14041 caggttagca atatgtcttt gaaaacagta gccgttatcg gcgcgatgga acaggaaatc
    14101 gagcttttgc gcgagatgat ggaaaatgtc aaagccgtct ctttcggcag attttccgcc
    14161 tatgaaggcg aattggcggg aaaacgcatg gtgcttgcat tgagcggcat cggcaaggtc
    14221 aacgcggcgg ttgcaacggc ttggcttatc cgtcaattcg caccggactg cgtcatcaac
    14281 accggcagcg caggcggttt gggcaagggt ttgaaagtcg gcgacgtggt gatcggcacg
    14341 gaaaccgagc accacgatgt cgatgtaacc gcattcggct atgcccgggg gcaagtgccg
    14401 caactgccgg caaggttcgc ttcagacggc atattgattg aaacggcaaa acgggcggcg
    14461 cggacgtttg aaggcgcgga ggtagaacaa ggcctgattg tcagcggcga ccgcttcgtc
    14521 cacagcagcg aaggcgtggc ggaaatccgc aagcacttcc ccgaagtcaa agcagtggaa
    14581 atggaagcgg cggcgattgc ccaaacctgt catcagttgg aaacgccgtt cgtcatcatc
    14641 cgcgctgttt ccgattcggc ggacgaaaag gcagacatca gctttgacga atttttgaaa
    14701 acggcggcgg caagttctgc aaaaatggtg gcagaaatcg tcaaatcttt ataaaacctt
    14761 tatcaaatca tgccaaaaca gcggttttcc gttagaatat cgggctattt ccacgttgcg
    14821 cccgatattc ggacgtaccg ctgtttgttc agacggcatt tctaaaacct accgttcagc
    14881 attaaaaaca actcaatgaa aaatatccga aatttctcca tcatcgccca catcgaccac
    14941 ggcaaatcga cgcttgccga ccgcttcatc caatattgcg gcggtttgaa tttgcgcgaa
    15001 atgagtacgc aggtgctcga ttccatggac atcgaaaaag agcgcggcat caccatcaaa
    15061 gcgcaaaccg ccgcactcaa ctacaaagca cgcgacgggc aggtgtatca gcttaatttg
    15121 attgacacgc cgggacacgt cgacttctct tacgaagttt cccgttcgct gtcggcttgc
    15181 gaaggcgcgc ttttggtcgt tgacgcgtcg cagggcgtgg aagcgcaaac cgtggcgaac
    15241 tgttacaccg caattgattt gggtgtggaa gtcgtgcccg ttttgaacaa aatcgacctg
    15301 cccgccgccg accccgagcg cgttgaacag gaaatcgaag acatcatcgg catcgatgcg
    15361 gtcggtgcgg tacagtgttc cgccaaaagc ggcatcggcg tggaagacgt tttggaagaa
    15421 atcgttgcca aaatccctgc gccgaccggc gacgaaaacg cgccgctgca agccgttatc
    15481 gtcgattcgt ggtttgacaa ttacgtcggc gtggtcatgc tgattcgagt gaaaaacggc
    15541 accatcaaac tgaaagacaa agtgcgcttt atgagcacca aggcggaaac gcaggtcgag
    15601 cagctgggcg tattcacacc gaaatcggtt caaaaacaag aactaaaagc cggcgaagtg
    15661 ggttttttga ttaccggcgt gaaagaattg ggacaggcga aagtcggcga tacggttact
    15721 ttggttgcca accctgccac tgagccgctg cccggtttcc aagaagtaca aagccaagta
    15781 tttgcgggtc tctatcctgt ggaaagccac gattacgaag ccttgcgcga cgctttggaa
    15841 aaattgcagc tgaacgatgc ttcgttgaaa ttcgagcctg aagtttctca agcattgggc
    15901 ttcggcttcc gctgcggctt cttaggcctg ctgcatttag aaatcgtgca ggaacgcttg
    15961 gaacgcgagt tcgacatgga tttgattacc accgcgccga cggtggttta cgaagtcgtg
    16021 ttgaaaagcg gcgaaaaaat cgaagttgaa aacccgtcca aactgcccga catcggcagc
    16081 atcgaaacca ttctcgagcc gattattacc gcgaccatcc ttgtgccgca ggaatacgtc
    16141 ggcaacgtca tgactttgtg taaccaaaag cgcggcgtgc aggtcaatat gcagtatatg
    16201 ggccgccaag tgatgctgac ttacgacttg ccgatgaacg aagtcgtaat ggactttttc
    16261 gacaaactca aatccacttc gcgcggttat gcttcgttgg actaccattt caaagaattc
    16321 caaccgtctg atttgattaa gctcgacatt atggttaacg gcgaaaaagt cgatgcctta
    16381 agcctgattg tacaccgtca aagcgcggtt cacaaaggcc gcgagctggc atcgaaaatg
    16441 cgcgaactga ttccgcgcca aatgttcgac atcgccgtcc aagccgccat cggcagccgg
    16501 attatcgccc gagaaaacgt taaagccctg cgtaaaaacg ttttggcgaa atgttacggc
    16561 ggcgatatta cgcgtaagaa aaaactgctt gaaaaacaaa aagcaggcaa acgccgtatg
    16621 aaacaagtgg gcaacgtgga aattccgcaa agcgcgttct tggcgatttt gcaggtgagc
    16681 gacaaataat atttcggcac atagggcggg cgcgtcggga acacctctca aaccgccctg
    16741 atttcagcgg cacgcgcccg caaacaaatt ttgcaatttg ccgccttaaa acggttgcac
    16801 ggccgggcgg gtttttaacc gttaaggaac aacaatgaac acaatgctaa tgtcgggcgc
    16861 ggctgccgcg ctgcttgccg gcatcatcct ttatttcaaa agcgacaaga aacggcagga
    16921 aaacggtgaa tggagttccg gcctcgaata cgcctatatc ctgacggcgg tcggtgtgtt
    16981 tgccgctttg tccctgttta tgagctttac cgccgttttc ctgattttcg ttgtattgtg
    17041 cggtacggct tggggggtat ataaataccg cctgaagact catcccgaaa tctcggaaag
    17101 cagccacttc ggcgattatt tcggcagttt cttccctacc gttttggtat tgttcctcat
    17161 ccggtcgttt attgccgaac cgttccaaat cccgtccagc tcgatgcgcc cgggattaat
    17221 caagggcgat ttcattttgg tcggcaaatt ttcctacggc ctgcgcgcac ccgttttaaa
    17281 caatgtattc atccccacgg gcaaaatcga acggggcgat gtcgttgttt ttaactatcc
    17341 tttgcagccg gagatgacct acatcaagcg tattgtcggc atcccgggcg acattgtcga
    17401 atatcgagat aaagttttga cggtaaacgg aaaaccggct tccgacattc ctgacggcac
    17461 ataccgttat cctgacgata ccgacccatc cgaaatccac aacacggata tgttccgcag
    17521 cggtctggac ggcaaatcct tcaatattct gaaaaaagaa ggacagcctg ccgtttccct
    17581 gcccgtattg ggcaaatata cctccgatat tatgtctgaa aacggatatt ccatagatca
    17641 aagcggtctg aaccattgcc aatatgccga cgacggcagc ggtttcgtgt gcaaagttcc
    17701 cgaaggacgc tatttcgcta tgggcgacaa ccgcgacaac agtgccgatt cgcgctactg
    17761 gggctttgtg gatgacaagc tggttgtcgg caaggcaatg ttcattttga tgaacttcgg
    17821 cgatttcggc agggccggca cggcaatccg ttaagccgga aaattacgcc ggaacatcaa
    17881 aaatgccgtc cgaaccctgt ttcagacggc atttttatca tcaaactcct tgtttcccta
    17941 ccggcagcct cggggataat atagtggatt aaatctaaac cagtacagcc tggcctcgcc
    18001 ttgccgtact atttgtactg tccgcggctt cgtcgccttg gcctgatttg aatttaatcc
    18061 actatatgca ccgccttgcc ggcattatcg atatgggcgg cacatatgga ttgcctggaa
    18121 aaatgatgcg ttcgggcggc gtatgatttg cctgtgttta aatcatcggt tgattttatc
    18181 cgtcggcaag tgaaaagccg gttatttccg tttgtgtgga aacccatccc attgaataaa
    18241 gggcatttcg tccgactgac ggtgtgggcg gcatatggaa atacggacat tattttagtt
    18301 gcattgccat tcctgcggag gcggcttttt tgattctgcc cgccccgatg ccgtctgaaa
    18361 gggcttcaga cggcattttc cgctccgcgc cgttgtcggc atgtttggtc ttgccggttt
    18421 tgactgcctg ccagtccaat cgtacgcagg tttgccacgg cagaaaatct gatgccgacc
    18481 ggagtcaaag ctcggacggc tgtttcagcg aaccgaggta aatcgccgaa agcgtcagca
    18541 ccacccctgc ccactgcacg ccttcaatcg ttttgcccaa tccgaaataa tcgatgaaca
    18601 gggcggcgac cggttcggac aaaagcaaca gccccgtcag cgacagggaa agcagcggaa
    18661 tcgcataggc aaccatcgcc cacgcaaaac actgcatcac cacgccccgt acacaagcac
    18721 caaacccgaa tctttccaag tggtcggata aagtgcgccg ccgtccatca gcaatgccgg
    18781 aacaatcggc gatgccgcgc cgcccaaact caaaatcatc attgaaggga aaagcgccac
    18841 ctgttcgact tcatgagtct tgcggacgaa caccatagat agggcaagca tcagcccgga
    18901 caccaaaccg ctggcgaatc cccaaaccgc attaccgttg tagccgaatt ccgcaccggc
    18961 aatcatcgcc acgcccacaa ccgccgacat caagcttgcc gcctgcagcc tgcccaaacg
    19021 ctcgccgaaa aagaaaacac cgattgccga caagaaaaag atttgcaggc tgttgagcag
    19081 ggtggaaata cccggcccga ccgcgtgtat gctttcgtgc caaagtgcca aatcgaaagc
    19141 gagaaacacg cccgccgtca gggcatatcg gacggttttt ctgttttttg ggaatttttg
    19201 tccgaaaaac cgtgccaaaa accaaaatac gaataccgaa atcagcaacc gccaaaatgc
    19261 gattgcatac gggccgacgg ggacggatct gacaatcagg ctgcccaatc cgaacaccgc
    19321 gcaacccagc accagcaatg gtgcggcata tttttttccg ttaccgacca caatatcccc
    19381 tcccttcccc atatggctgc cgcgccgccg gcaaaagtgc cgccattccg aaaatatgcc
    19441 gtctgaagcc caaagccccg gctgcgcccg gacaaacggc tgactttacg ggcaggccgg
    19501 ctattttccg ttggggcaaa atccgtcctg ccggataaaa cccgaataaa aaatatttcc
    19561 ctcaagcaat gcggcggcgc caacacctga ttaaaacaca accggcgttt tcggtatata
    19621 tgcaaaaacg cattttccgg cacggcgtta caccgatacc gaaaaatgcg tctgaatgtt
    19681 tcgacaatcc gattaaagca ttatgccaaa tctgcaaaaa gtggcgtaga gagatagcgt
    19741 tcgccataag aaggcagcag cacgactatc agcttgcctt cgttttcagg ctgtttggca
    19801 agctgcaacg cgctccaaac cgccgcaccg gaagaaatgc ccgccaaaat gccttctttt
    19861 tccgccattg cacgggcggt ttcaaaagcc gcttcgttcg gcactttggc aatgctgtcg
    19921 tagattttgg tattcaaaac ggtcggaata aaacctgcgc cgataccttg aatcgggtgc
    19981 ggaccttttt cgccgccgct caatacgggg gaagcctcag gctcgacggc aaccacttta
    20041 acttcgggtt tgtatttttt caatacttcg cccacgccgg taatcgtacc gcccgtgccg
    20101 acgccggcaa cgaagacatc gactttaccg tcggtatcgt tccaaatttc ctcggcggtt
    20161 gttttgcggt ggacttcggg atttgcctca ttgtcgaact ggcgcggcat aaaataagtg
    20221 tctggatgag cgtccaccaa ggattgcgct ttggcaatcg cgcccgccat accttcggcg
    20281 gcgggggtta gaatcagttc cgcgccaaac gtgcgcaaca gcattttgcg ctctttgctc
    20341 atgctttccg gcatggtaat cgccagtttg tagccgcgtg cggcacatac cattgccaaa
    20401 ccgatacccg tattgccgct ggttgcttcg acaatgacgg tgtttttgtt gatttttccc
    20461 gctttttcgg cggcctcgat cattgcttcg gcaatgcggt ctttgacgct gctgcccgga
    20521 ttaaaaaatt ccagtttcac ggcaacctct gccttcaaac ctttggtcaa acggttcagt
    20581 ttgaccaaag gcgtgttgcc gatcaattcg gtgatgctgt ttgcaatttt catcttctgc
    20641 tcctgaataa taaggtttcg acagtaaggc gcaatcccca tcggtatcct gtccgattgc
    20701 aggggatttg cgccgaacca taaacaaatt tcggatatag ctttatccta aacctatgcc
    20761 gcctaaaaaa ccaataccca aaatttattt ctttataact ttttgttccg atacagacgg
    20821 catcacaatc atctgccgat ttcggcaagc agcatcgccg ttaccgcaac ctgcatcaac
    20881 atcgccgcca acacgccgag tcgcagctgc gaaccgccga acgttgacgg cggctgccgg
    20941 tctataccgc agcggtggac ataggcaacc gccatcaata caaactgcaa cacgaaccaa
    21001 taaaccggct tcagcgcata aagtttgagc aatgccgcaa acacaaacac ttgaaccgtt
    21061 cccgaaaagc aataatgcac caacgcacat attccgacaa aagcaagcgt catcgataca
    21121 ttactgacgg caaaaagccc gagcagcgga tgttcggggt ctgcctgcca tccgtttcca
    21181 tctgttttcc ggttccaata cccgatgaaa aaaaatatgc tgcccaaagt caggtaaaaa
    21241 tgggggatga acaaaggcgc ggcgcgggtc attccccaca tccccggcat cagctttacc
    21301 cctaaaaccg agatgccgag ccacaacgca atactcgccc aaaaccattg caacattcct
    21361 gcgcggaata tcagaaacag aaaaaccgcc gcatacagta aggcagacaa aaaatccaaa
    21421 tgcagcaaaa tcatgaatac gccaactcgc cttcaaacac ggtttccgca gggccggtca
    21481 tcatcacatc gccgccgcag gcccattcga tatataaagt cccgcccggc aaaaccacct
    21541 ctaccgtttt cccttcatcc aacagcccca gacggatacc cgccaccaca gccgcacacg
    21601 cgcccgtacc gcacgcttgg gtttcgccca cgccgcgctc gaacacgcgc aaacggattg
    21661 ccgtccgtcc gacaacctgc ataaagccga cgttgacgcg ttcgggaaac tgcctgtgcg
    21721 gttcgatacg cgaaccggtt tcgcgcaccg gcgcgcattc cacgtcatcg actacaatca
    21781 cagcatgagg attgcccata ttgacgcagc tgacaggctg aatgccggat tcgagatgca
    21841 ccccgtaaat acaggcatca tccccctcgc ccgattcggg gacaaacggt atttcagacg
    21901 gcataaactt cggtttgccc atattgaccg taaccatacc gttatcggac aatttcggaa
    21961 aaataatgcc cttggccgtt tcaacacaaa tttctttctt atcggtcaaa cccttgtctg
    22021 caacaaaacg ggcaaaacaa cgcgctccgt tgccgcattg cccgacctcg ctaccgtcgg
    22081 cattgaaaat acggtaacgg aaatccacgc cttcggtttc cgaacacccg accaccaaaa
    22141 gctggtcgaa gcccacgccc cggaagcggt ccgcccatgc cgcaatcggc acgtcctcgg
    22201 gggtaaaatc ctgactgacc gcgtcaatca ccataaaatc gttgcccaaa ccgtgcattt
    22261 tggtaaattt caaagttttc atattgttcc gaaatcccgt attgtctgtt gcttccgaat
    22321 ccgaaatccc cgagcccgaa gggaaatgct tccatctccc gccgcctgag gattaaaatg
    22381 agccattata ctgtttgtta ccccttacaa gaaacacaat gtcccgcctg ttcctgctgc
    22441 ttttcccttg ggcaaccctt gccgcctgca cccgaaacat accggcaagc cccgccgttt
    22501 caaattgcgc gcatcttcac aaccgcaccg tcctgtattt tgacgcacac ggcaaacccg
    22561 ccgacagcac agcaaagggc ggattttaca ggaccgtcga aattcatgac acaaaccgca
    22621 tccttattca agactttaac agcacgggga atgccctcgt caaaccctat tggtcggact
    22681 gccggaaatt gtccgttttc cccgacaaac atctgaccat gagccatcca tgaaccacga
    22741 catcactttc ctcaccctgt tcctgctcgg tttcttcggc ggaactcact gcatcggtat
    22801 gtgcggcgga ttaagcagcg cgtttgcgct ccaactcccc ccgcatatca accgcttttg
    22861 gctgattctg ctgcttaaca caggacggat aagcagctat acggcaatcg gcctgatgct
    22921 cggattaatc ggacaactcg gcatttcact cgaccaaacc cgcgtcctgc aaaatatttt
    22981 atacacagcc tccaacctcc tgctgctctt tttaggctta tacttgagcg gtatttcttc
    23041 cttggcggca aaaatcgaga aaatcggcaa accgatatgg cgcaacctga acccgatact
    23101 caaccggctg ctgcccataa aatccatacc cgcctgcctt gctgtcggaa tattatgggg
    23161 ctggctgccg tgcggactgg tttacagcgc atcactttac gcgctgggaa gcggtagtgc
    23221 gacaaccggc ggactgtata tgcttgcctt tgcactgggt acgctgccca atcttttggc
    23281 aatcggcatt ttttccctgc aactgaaaaa aatcatgcaa aaccgatata tccgcctgtg
    23341 tacaggatta tccgtatcat tatgggcatt atggaagctt gccgtcctgt ggctgtaagc
    23401 cccattcatt cccgataccc gtaaaaaata ccgtctgaaa cctgttcaga cggtattttc
    23461 accctaagcg ttactcggcg gcattttctt cacggacagg cgcatccagc agggctttga
    23521 tggacagacg gacacggcct ctgtcatcca cttccaatgc tttcacgttc accacctgac
    23581 cgacttgcag gtaatcgccg acattgcgta cgcgctcgtg tgcgatttgg ctgatgtgta
    23641 ccaaaccgtc tttgcccggc atcacgctga caatcgcgcc gacgttgttg tcgaggattt
    23701 taaccacagt accttcgtac actttgccca cttccacttc ggcagtgatt tcttcgatgc
    23761 gttttttcgc tgcatcgccc gcttcttgag tagtcgcagc gatagtgatg gtaccgtctt
    23821 cagcgatatt gatttcagta ccggtttcag cggtaatcga acgaatggtt tcgccgcctt
    23881 taccaatcac atcacggatt ttgtcttggc tgattttcat ggtgaacaaa cgcggcgcgt
    23941 gtgcggacag ctcttgcgga cccgcaacgg cagctttcat ctgatccaag atgtgcaaac
    24001 gcgcttcttt ggcctgtgcc aaagcaattt gcataatctc tttggtaatg ccttggattt
    24061 tgatgtccat ttgcagcgcg gtaacgcctt cggtcgtacc ggccacttta aagtccatat
    24121 cgcccaagtg gtcttcgtcg cccaaaatgt cggtcaggac ggcaaactta ttgccttcca
    24181 aaatcagacc catcgcgata ccggcaacgt gtgctttcaa aggcacgccg gcagacagca
    24241 ggctcaaaca gccgccgcag acagaagcca tagaggaaga gccgttggat tcggtaattt
    24301 cggagaccac gcgcatggtg tagctgaaat cttcaggttt cggcaatacg gccaacaacg
    24361 cacgtttagc taaacggccg tgaccgattt cacggcgttt cggcgcgccc atgcggccca
    24421 cttcgccggt agagtacggc ggaaagttgt agtgcagcat aaagcggtcg gtatattcgc
    24481 cggacaacgc gtcgatgatt tgttcgtcgc gcgaagtacc caaagtcgct acggcaaggg
    24541 cttgggtttc gccacgggta aacagtgcgg aaccgtgcgt gcgcggcaat acgccggttt
    24601 ggatgttcag cggacggacg gtgcgggtgt cgcggccgtc gatgcgcggt tgtccgtcca
    24661 aaatttggct gcggacgaca tcggcttcca agcgtttgaa aatacctttg atttcgttgg
    24721 ctgccagcgt gtcggtttct tcggtaatca aggcttcttt taccgcattc caagcctcgt
    24781 ccaatttggc ggaacgcgct tgtttttgac ggattttgaa cgcttcttta atggtttcgc
    24841 cggcaatcct gcgtactttg gcaaccagtt cttcattggt ttcaggtgct ttccaatccc
    24901 aaacttccgg attcacttcg tcggcaaatt cattgattgc attgatggca acctgcattt
    24961 gatcgtggcc gtaaaccacc gcgcccaaca tcacgtcttc gggcaggatt ttggcttcgg
    25021 attccaccat caacacggct ttggaagtac cggcgaccac caagtccaat tgtgatttcg
    25081 ccaactcggc tttagtcggg ttcaaaacgt acacgccgtt tatataaccc acgcgcgccg
    25141 cgccaatcgg gccggcgaac ggtacgccgc tcaacaccag cgcggcggac gcgcccaaca
    25201 ttgcaggaat gtcggaatcg atttcgggat cgacggacac aaccatcgct acgatttgga
    25261 tgtcgtggta gaaaccttcg gggaacagcg gacgaatcgg gcggtcgatc agacggctgg
    25321 tcaggatttc tttttcgctt tgtttgcctt cgcgtttgaa gaagccgccg gggattttgc
    25381 ctgcggcata agtgcgttcc aaataatcga cggccaacgg gaagaagtcc tgaccttctt
    25441 tgacctcttt gttggtggta acggcaacaa aaacaacagt gtcgcccatc gatactttaa
    25501 cggcagcggc agcttggcgg gcaatttcgc cggtttccaa agtaacggtt tgattaccgt
    25561 attggaaggt cttaacgtat ttattgaaca tcattgttcc tttcaaaata ccgcactgct
    25621 aaaacactaa taatgcacac taaaatccga atgtgcatag ttagggtttc agaccgtgcg
    25681 gcaggttgta aacaagcttt cagacggcat ttcggacgct gaaagcaatt gcggattata
    25741 aaggcaacca tccttaaaat ccagtattga tacaataaaa aggccgtctg aaacatatcc
    25801 ttttcagacg gccttcagtc ttcaaaatca atcttgtgtc agtaaagtca atgcttcctg
    25861 atatttctcg accgttttcc gaattacatc ggcaggcact ttaggcgcag gggctttttt
    25921 gttccaaccg ctttgttcca gccagtcgcg gacgaattgt ttgtcaaaag acggcggatt
    25981 gatgccgact tcatattgat ccgccggcca gaagcggctc gaatcgggag tcaatacctc
    26041 atccatcagc gtcagcgtac cttcttcatc caaaccgaat tcaaatttgg tatcgcaaat
    26101 gatgatgccg cgcgatttgg catattccgc cgcttcggtg taaagccgaa ccgctttgtc
    26161 gcgcacttct tccgccaatt ctttaccgat aatgcgtccg cattcttcaa agctgatgtt
    26221 ttcatcgtga tcgccgactg cggctttagt tgagggcgta aaaatcactt cgggtagttg
    26281 ttgcgcttcc cgcatacctt cgggcagccg aataccgcaa accgagccgg ttttttgata
    26341 atctttccaa ccgctgcctg ccagataacc gcgcacaatg gcctccactt tcaccggagt
    26401 gagtttttta gctacaacgg cgcgtttttc caaagctttg gcttcgtttt caggcaaaac
    26461 atcgtacacg gtatcgccgg tgaaatggtt gggcataata tgcgccagtt ttttaaacca
    26521 aaaattggaa atctgcgtca ggatttcgcc cttacccgga accggggcgt ccaaaatcac
    26581 atcaaacgcg gacaggcggt cggaagcgac catcagcata cgtttatcgt cgatttcata
    26641 taaatcgcgc acttttccgg aataaatctt taccaaacca atctcactca tttcgcaccc
    26701 ccctgaaaat atcttgaaaa taccgacccg acaggtttga atcgcaaacc gatattctag
    26761 ccgaagtcgg cgcaaaacaa tacccatggc acaaaaagcc aacccgtcaa ccgtcggcaa
    26821 agttttggca ctataatacc gacagcaagt cctacaatac actttcacca aaggaaatac
    26881 ctcatgagaa tcctattgac aggctcgaaa agccaactgg cacactgcct gcgcgaccgt
    26941 cttcctgaag actgggaaac cattgcgacg gattccgcat ccctagacat taccgatgcc
    27001 gatgccgtct gcaacatggt caaaagtttc caacccgacg ctattgtcaa cacggctgcc
    27061 tatactgccg tcgacaaagc ggaaggcgat gcggcagcgg catttgccgt caatgcttcc
    27121 gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat ccacatctca
    27181 accgactatg tctttgacgg aaaagggaaa ataccctatc aggaaagcga ctttaccaac
    27241 ccttccaatg tatacggaca atccaaaacc gccggcgaac tgctcgcact gtctgccaat
    27301 cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga caactttgtc
    27361 cgcacgatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca caaccaaatc
    27421 ggctgcccga cctatgccgg cgacttgtcc gccgccatca tccgcctgtt gcagcagtcc
    27481 aatcccgttc gcggcattta ccactacgcc ggcggcaaat ccgtatcctg gtacgaattt
    27541 gcccggcaca tctttcagac ggcattgcaa caggatgcct cttttcccgt tcccgaactg
    27601 aaagccgttt cggatgaagg ctctgcggct gccgccccca ggcccgcata cagcattttg
    27661 gactgccgca aaatcgaaaa cgacttcggc atcaaaccgt cagactggca aaaagccctt
    27721 gcacaggtcg tttccaagct gctctgatgc cgcccgcccc tccgtttcct ccgccaagca
    27781 ccgccttggc ggttttctta taaaatctac tgattacaca aacacatatc atctttacac
    27841 aatcatgctt ccatcaacag caaaacatga tatgattgcc aacaatgaca tctcacaata
    27901 aattttctaa tttttattga aaaaatcaat aaattaagaa ttctcccaac cgacaaataa
    27961 aataaagaaa gggtcaatat gcaacaccgt agaagattgg caatttacca ggcatccaaa
    28021 cgtgcttcct ttaccggcag gtcatcagcc ccacaaaaac gtaaagaacg ttgatttgaa
    28081 aaaatgccgt ctgaagtcct gcttcagacg gcatttttta ccgttcgaga aactgtttca
    28141 acctgtcctc atccaaaaac cagtggcaaa tttcctcatc tcccgccaac aaaacgggaa
    28201 tcagctcatt gtatttttct tccaaaacag gattttcatc cacatcgacc acttccagcc
    28261 cgaacccgta ttcatcctga aaaggtttga gtgcatcgcg cattttgtgg cacaagctgc
    28321 aatattcacg aaacatcaag gtcaatttca tccgcgtttc cttatctgtc aatttgcaca
    28381 cgccaaagcc ttagacgcag cagaatcatg gtctatttgg gaaaaaacaa tgtcttcgag
    28441 gaagatgata ctcaagtcct gccaaaaaca gcaaaaatgc cgtctgaaca gttcagacgg
    28501 catttccaaa accgttttac gcttgaacgt tgatacccgc cgtaaccgtc ggttgcgcgt
    28561 tgtgcgccgt cagaaacgaa gcggcaagct ccccgcagca gccgcagcag ctcgccacgc
    28621 ccaattgcga ctgcaaatcg cccattgtgg tcgcgccggc agcgatggtt tccttgattt
    28681 gatggtcggt aacggcattg cagatgcaga caaacatttt gtgctccgtg tgttctcaaa
    28741 ctatcgtacc gatagcggct tttattatcg tatgcaaata taaataaaaa cggttcgcat
    28801 tgcaaggtcg gcatacacgg tttgtcttgg taatttttta tcaagtttgc attttcgatt
    28861 gatttcttat taaaccgaag cagaagcctc agtttcggga atggtgttcc ttaaccacgc
    28921 tatgcagccg cacattggca acatgggtat aaatctgagt cgtgttcaaa tcggcgtgtc
    28981 ccaacatatc ctgaactacg cgcaaatcca atccgtgccg caccagatgc gtggcaaagg
    29041 cgtggcgcag gctgtgcggg ctgatgtgcc cgatgcctgc cctgccggca tattctttga
    29101 caatcatcca cgccaactgc cgggaaatgc ccgttttttt ctgactgaca aacaatgcgt
    29161 cgcaactcct gcctttcagc agaagcgggc gtgcctccgt ataatagcgt tccacccaat
    29221 acgctgactc ctgtcccatc gggaccatcc tctgcttgtc gcccttgccc agcgtggtaa
    29281 tacaacccct gtccaaatcc acattgccga aattcagccc gaccgcctcg ctgacgcgca
    29341 agccggtcgc gtacatcaat tcgagcaggg ccttatcccg caaaccgtgc ggcgtgtcgg
    29401 tatccggtgc ggcaagcagt cgggaaatct gctgttctgt aatcagtgtc gggatgtttc
    29461 tgtcgatttt tggcggtttc agcaggcggg tgggattgtc cgccctcatc ccttcccgtt
    29521 ccatccacac atacaggcgt ttgcacgccg acagtgcgcg tgcctgcgaa ctccgttgct
    29581 ccccgtcaac ataaaccgcc gccgccaaat ctgcttcgtc cgcatccttc agcgttctgc
    29641 cgcacaaaga cagccggcgg gcgatctttt ccaaatcccg ccggtagctg tccaaagtat
    29701 tccgactgag ccgccgatcc aaccacaaag tttccagcag cctgtcaatc aaaccgtttt
    29761 ccatagcgtt ccaaaccaat gccgtctgaa tcttcttcag acggcatggt tcacatcatc
    29821 gggaaagcgt ttccaacact tcctgtgcat gacccgccac tttgactttc cgccactccc
    29881 ggacaatttc gccgtccttg ttcagaacga acgtactgcg ctcgatgcct aacgactctt
    29941 tcccatacag ttttttcaat ttgatgacat caaacaggcg gcacaccgtt tcatccttgt
    30001 cgctcaacag ctcgaaccgg aaaccctgct tggcgcaaaa attctgatgc gcctttacgc
    30061 cgtcgcggga aataccgacc acggtataac ccaatgcctc aaactgttcc aaacgcgcat
    30121 taaaatccaa gccttccgtc gtacagcccg gcgtactgtc tttcggataa aaatacacga
    30181 ccaaaggcag atgttctgcc gaatgaaaat ccgcaccgct gctcgaaggc agggtaaatt
    30241 catatttcat atccatagtc ctactcccga tattcccatt attcaaaacg gcacgcagac
    30301 gcaccgccgc aattgccaaa ccaaccccga ttctaccgcc ccaaaggaca aggattcaac
    30361 cgccggaaac atccaaaccg acacacgacg gcatgaaaaa tatccatgtc aaaccacaaa
    30421 atatgttccg atttaaaaac agaatgttat aaaacccaac tccaaaaaca caacaagacc
    30481 gcccgcctac gggcagtctc ctgtcagacg acatacttta cagatggctg ttttttcaac
    30541 aaaataacgc caatactcaa aaatacggaa ccaaaaatgt ccatccgcac cctgaaacgc
    30601 ctgccctcat cgctgctgct cggcctctgc ctttccctgc cgtcagccca cctttttgcc
    30661 gacaacgaca ttttagggca atttttagaa cagaacatgc ttacctcctc cgatccgata
    30721 gaaatattcg ccgaaagcac gatatatccc gccgataccc aggccattac gggcgggctg
    30781 attctgtcct cccaatccgc actggtcgtc aacaacaaaa cagggcaaat actgtatcag
    30841 aaaaacgccg acaggattat gcccattgcc tccatttcca agctcatgag cgcgatggtc
    30901 gttttggatg caaacttgga catgaacgaa accgttacca tcacacccga cgaaatcgac
    30961 cgcctcaaag ggaccggcag ccgtcttgcc ataggtacgg cacttacacg taaagaactg
    31021 ctgcacctta gcctgatgag cagcgaaaac cgcgccaccc atgcattggg cagaacctac
    31081 cccggcggca tgggcgcatt tgtcgccgcc atgaaccgca aagcccaaag cctgggcatg
    31141 tacggcagcc gcttttacga accgaccgga ctcaacttcc aaaacgtttc caccgccaaa
    31201 gacctgagcc tcatggtcaa cgccgccgcc caatatccgc aaatccgcac caactcgact
    31261 tccaactacg cctcggtaca aaccaaaaac gggcagcaaa actataaaaa ctccaatgcc
    31321 ctggtcaggg aaggcatgtg gaacatcgaa ttgcagaaaa ccggctacat ccgcgaggca
    31381 ggcaggtcta tggttgtcaa agccaacatt caaaaccaac ccgttaccat cgtattgctg
    31441 aactcgccca catccgccac acgcgtcaac gacgcccgca aaatcgaatc gtggatgctg
    31501 cagcaacgct cctgacatac aaatgcccgg cggaaaaccg cccaaacaca aaaaaattgg
    31561 taatttttcc tattccaggc tacaattcga cacgcacagg gcatctgccc gatttcacaa
    31621 tcataatagc ggagttaaaa aatatgacag ctaaaggaca aatgttgcaa gatcccttcc
    31681 tgaacgcatt gcgtaaagag catgttccgg tttcgattta cttagttaac ggtatcaaat
    31741 tgcaaggtca ggtcgaatct ttcgaccaat acgtcgttct tctgagaaac acttccgtaa
    31801 cccaaatggt ttacaaacac gccatttcca ccatcgtacc ggctcgctcc gtcaacctcc
    31861 aacatgaaaa caaaccccaa gccgcaccgg cttcgaccct tgtccaagtg gaaaccgtcc
    31921 agcagcctgc cgaataatcc gcacgaagca tgacgtgtca tatctttcaa taccttaccg
    31981 gacaacggta aggtattttt attttcaggc ggcatttaaa aatattattg aaaaacatcc
    32041 ttccattcgc ccattgcctt ttgcgggagg cacttcccga aggcggcaat gcgctggacg
    32101 gcaccgccgg caacggacac gacacccttt tcctcgcaca aaccgcaggc aacaggggga
    32161 aagtgtgggc attcgacatc cagccgcaag ccctgaacaa cacccgatgc cgtctgcagg
    32221 aagcaggtta cagcaatgta cggctcatct tggacggaca tgaaaacctg aagcaatata
    32281 ttccggagcc gctggatgca gccgttttca atttcggctg gttgcccgga ggcgacaaga
    32341 atctgaccac cgacaaggaa accagcattc ccgccctttg agcaaccctc tccctgctga
    32401 aagaagacgg tatgcttatt gccgtcctct atccgggaca cgaaaacggc aaacaggagg
    32461 cagaagccgt cggacaatgg gcaaaaaacc tgcctcaaga acagtttgcc gttttacatt
    32521 acggctttac caaccggaag aatagcccac cctatctttt ggcatttgaa aaactgcgtc
    32581 aaaaataact gtttgcggta aaataagccg attctaaccc ttattttgat acgagccgac
    32641 catgcagtat ctgattgtca aatacagcca tcaaatcttc gttaccatca ccattttggt
    32701 attcaacatc cgttttttcc tactttggaa aaatccagaa aagcccttgg tcggcttttg
    32761 gaaagcactg ccccacctca acgacacgat gctgctgttt acgggattgt ggctgatgaa
    32821 gattacccat ttctccccgt tcaacgcgcc ttggctcggc acaaaaatcc tgctcctgtt
    32881 cgcctacatc gcactgggca tggtaatgat gcgcgcccgt ccgcgttcga ccaagttcta
    32941 caccgtttac ctgctcgcta tgtgttgcat cgcctgcatc gtttaccttg ccaaaaccaa
    33001 agtcctgcca ttctgaaaca ctgccatgaa caacagacat tttgccgtca tcgccttggg
    33061 cagcaacctt gacaaccccg cacaacaaat acgcggcgca ttagacgcgc tctcgtccca
    33121 tcctgacatc cggcttgaac aggcttcctc actgtatatg accgcacccg tcggttacga
    33181 caatcagccc gatttcatca atgccgtctg caccgtttcc accaccttgg acggcattgc
    33241 cctgcttgcc gaactcaacc gtatcgaagc cgatttcgga cgcgaacgca gtttccgcaa
    33301 tgcaccgcgc acattggatt tggacattat cgactttgac ggcatctcca gcgacgaccc
    33361 ccgccttacc ctgccgcatc cgcgcgcgca cgaacgcagt ttcgtcatac gccctttggc
    33421 agaaatcctc cctgatttta ttttgggaaa atacggaaag gttgtcgaat tgtcaaaacg
    33481 gctgggcaat caaggcatcc gtcttttacc ggacaggtaa ttccgcacgc ggatgccgtc
    33541 cgaacaccgg tttcagacgg catttttcct ttgccgccaa cacgcgtgca aaaaaaatcg
    33601 ccccttgaga aaggggcgca aaaggaacac aaaccactac caaaacttta aatctgcaac
    33661 actgcctgcc gcatactgta cccgacagga tataaggccc tcactaaatc gtttcgagaa
    33721 atccaaactt cttcatcacc gacagaaaat ctgcctttct ccggcaccag ctccaacaaa
    33781 aacggctgaa ccgccgtatg cagcttgtcc ttacaccgcc ccagctttct ggacaacgcg
    33841 gacagggcgc gtttgtaggc attatccttg cggtcgagct ccctcgcact accgacccag
    33901 ctcaaacgaa ggttgcggct ttcatcttca atcaacaaaa tacctgtttc aacttccccc
    33961 acctcgggtt cgcacgaaag cgcaatataa tatttgccgt caatattgac atatgccgct
    34021 tcatgcacct gacagttgca acccgaggtt gcgagcaggg aataaacaga atcacggtgc
    34081 agcctcaaaa tatcggagga aatctgtttg aaaatacttt ccggcatttt tccctccttt
    34141 cgtctgctgc gttgtttact ggattgactg cattatacag acatctccca attttacaaa
    34201 tgataattgt tcctattttt taaaaagttt ataaattatt gataaatata tgaattaatt
    34261 tttaagattt aaaacctttg tctcaaaaaa tacaaaaatg ccgtctgaaa cgtgttcaga
    34321 cggcattggc ttcacaggca gtccgtttaa aatttcacgc ccttatgcag cgcgacgatg
    34381 cccgcactca tattgtgata atccacgctg tcgaagcctg catccagcat catctgtttc
    34441 aaagtttcct gatcggggtg catacggatg gattcggcga gatactggta gctgtccgca
    34501 tctttggcaa tcagcctgcc catcaccggc aacagcttga aagaataaaa atcgtatgcg
    34561 ccttcgagcg gcttgtagat tttggaaaac tccaacacca gcaacgtacc gcccggtttc
    34621 aaaacacggt acatttcctt aagcgcggca tctttatacg tcatgttccg caagccgaac
    34681 gccacggaga ccaagttgaa ataattgtcg gggaaaggca gtttttccgc atcggcaagc
    34741 gacacgggta aaatcatgcc ttcgttcaac agacggtcgc gcccgacggt cagcatagaa
    34801 gaattaatat cggtcagcca aacctcgcct tccttgccga cccgtttcgc ccaaccgcgc
    34861 gacaaatcgc ccgtaccgcc cgcaatgtcc aacactttat cgcctttttt caggtgcgcc
    34921 gtatggatgg taaaatgctt ccatacgcgg tgcagccctg ccgacatcac atcattcata
    34981 atgtcgtagt ttttggcgac ggagtgaaac acttccgcca cttttccggc tttctcatct
    35041 tcgttgaccg tgctgaaccc gaaatgcgtt ttttgtccgc ccatattcta accacctccc
    35101 tgataccgcc ctacggcatc ccaaacttaa caatctgatt gcttttcaaa acgcgacgcg
    35161 cccgccgccg ccaatttgtc caaatattcc tgccacatcg catcgtatcc gtatgccagt
    35221 ctgtgcagat acgcccaatc gtaaagaccg ctgtcgtgcc cgtccgaaaa actgattttc
    35281 agcgcatact gtccgacagg ctgcaaatcc gtaatctgca catccgcctt accggtctgc
    35341 aaaacatcct gtcccacgcc gtgtccgcgc acttccgcac tcggcgaata gacgcgtaaa
    35401 aattcggcag gcagattttt aggttcgtcc ccataagtca aaaccaacac ggcacggttt
    35461 ttcaacaaac ggatttcaac aggaatatta ttcaaaaaac acctcggttc ggcatacaat
    35521 atccggcagg cgtacgcgtg cctttaaacg gcgcgtattt taacagcttt atcccgatac
    35581 cgatattcaa acccacactt cagacggcat tgatatggac aatctcgtat gggacggcat
    35641 tcccgacatc cgcacactcg accaaaccat ccgcaaacac gcacacccgc tcaacctgat
    35701 tgtctgcctc cccgataatc agattcccga ttttcaaacc gcacaagatg cttcggactc
    35761 ggaatgccgt ctgaagcacc gtttggatca ggcaacccag tgcctccagt tcgacagcat
    35821 caacctcatc gaacacatcc tgcccgatgt ccgcttctgg ctggttcccc cttcacgcac
    35881 ccgccgcctg cacgaacact tccaccacat ttcctggcag accgaagcca tcccgcaaac
    35941 cgaaagcaag tccgacaaac cctggtttgc acttccacaa acatccgaac ggaaaaaacc
    36001 ggaacacgtc ctcgtcatcg gtgcaggcat tgccggcgca tcgaccgccc acgccttagc
    36061 atcacacggc atttccgtta ccgtattgga agcccgaaaa gccgctcaag ccgccagcgg
    36121 caaccggcaa gggctgcttt acgccaaaat ctcgccgcac gacaccggac agaccgaact
    36181 gctgcttgcc ggctacggct acaccaaacg cctgctcgga cacatcctgc ccgactccga
    36241 cacttggggc ggcaacggca tcatccacct caattacagc cgcaccgaac aacaacgcaa
    36301 tcacgaattg ggtttgcaaa aacaccataa ccacctctac cgcagcatca cgtctgcaga
    36361 agccgaaaaa atcgccggca tcccgctgaa cacgccctac gccgaaccat tatgcggact
    36421 ctactggcaa cacggcgtat ggctcaatcc gcccgcattc gtccgcaccc tcctcagcca
    36481 tccgctgatc gaactatatg aaaacacaac gttaaccggc atttcccacg acggagaaaa
    36541 gtggattgca agcacgccaa acggcacatt taccgccaca cacatcatct actgcaccgg
    36601 cgcgcacagc ccctgcctgc ccgaaaccaa cctcgccgcc ctacccctca ggcaaatacg
    36661 cggacaaacc ggcctcacac cgtccacccc gttttccgaa caactgcgtt gcgccgtttc
    36721 aggcgaaagc tacatcagcc cgtcgtggca cggactgcac tgctacggcg cgagttttat
    36781 tcccaacagc agcaataccg gatggaacga agccgaagaa gcctcaaacc gccaagcatt
    36841 ggcacacctt aaccccgccc ttgccgaatc attgtttgcc gccaacccaa acccccaaaa
    36901 acaccaaggg cacgccgcca tacgctgcga cagccccgac cacctccccg tcgtcggcgc
    36961 actcggcgac attgccgcca tgcggcagac ttacgccaaa cttgcactgg acaaaaacta
    37021 ccgcatcgac accacatgcc catatctgcc caatgcctac accaataccg cccacggcac
    37081 acgcgggctt gccaccgccc ccatctgcgc cgccgccgtt gccgccgaaa tcctaggctt
    37141 gccccatctc ttttcacaac gcctgcgcca cgccctacac cccaaccgca ccgtcatccg
    37201 cgccatcgtc agaaggcaga acctgattcc ttaagccttt gccgcatgcc gtcccgacca
    37261 caaaaaatca tattcaaata aacataccaa tcctcacggt aaaatatcga aaacacccat
    37321 aacgttttct tcccaaaata aggagtaagg aatgcctaac aaattgcctt tgctgtccaa
    37381 gatgaatttt ccgtaacatt cgaagacttc cttaaccctg ccagccgctt cggtttctgt
    37441 accccctgtt agataaggaa gggatgaaaa agccctgtta cttgtatgaa gcaaagggcg
    37501 ttaatttttg ttaatcttcc cttctcagga actcaatata taaggatgtt aatttttatt
    37561 tcccttctcg aggactcaat ag
//
LOCUS       DAXFHF010000016        34387 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXFHF010000016 DAXFHF010000000
VERSION     DAXFHF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34387
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.016"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(145..1035)
                     /gene="sucD"
                     /locus_tag="ACFIRM_000690"
     CDS             complement(145..1035)
                     /gene="sucD"
                     /locus_tag="ACFIRM_000690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC3028364.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTNLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1046..2266)
                     /gene="sucC"
                     /locus_tag="ACFIRM_000691"
     CDS             complement(1046..2266)
                     /gene="sucC"
                     /locus_tag="ACFIRM_000691"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC3028365.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            complement(2284..2571)
                     /locus_tag="ACFIRM_000692"
     CDS             complement(2284..2571)
                     /locus_tag="ACFIRM_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028366.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(2691..4124)
                     /gene="lpdA"
                     /locus_tag="ACFIRM_000693"
     CDS             complement(2691..4124)
                     /gene="lpdA"
                     /locus_tag="ACFIRM_000693"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3028367.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(4385..5566)
                     /gene="odhB"
                     /locus_tag="ACFIRM_000694"
     CDS             complement(4385..5566)
                     /gene="odhB"
                     /locus_tag="ACFIRM_000694"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC3028368.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(5666..8494)
                     /locus_tag="ACFIRM_000695"
     CDS             complement(5666..8494)
                     /locus_tag="ACFIRM_000695"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC3028369.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(8699..9982)
                     /gene="gltA"
                     /locus_tag="ACFIRM_000696"
     CDS             complement(8699..9982)
                     /gene="gltA"
                     /locus_tag="ACFIRM_000696"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC3028370.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHTMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10094..10342)
                     /locus_tag="ACFIRM_000697"
     CDS             complement(10094..10342)
                     /locus_tag="ACFIRM_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC3028371.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(10346..11053)
                     /locus_tag="ACFIRM_000698"
     CDS             complement(10346..11053)
                     /locus_tag="ACFIRM_000698"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC3028372.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11173..12936)
                     /gene="sdhA"
                     /locus_tag="ACFIRM_000699"
     CDS             complement(11173..12936)
                     /gene="sdhA"
                     /locus_tag="ACFIRM_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC3028373.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(12939..13280)
                     /gene="sdhD"
                     /locus_tag="ACFIRM_000700"
     CDS             complement(12939..13280)
                     /gene="sdhD"
                     /locus_tag="ACFIRM_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC3028374.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13274..13651)
                     /gene="sdhC"
                     /locus_tag="ACFIRM_000701"
     CDS             complement(13274..13651)
                     /gene="sdhC"
                     /locus_tag="ACFIRM_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC3028375.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(13938..15341)
                     /locus_tag="ACFIRM_000702"
     CDS             complement(13938..15341)
                     /locus_tag="ACFIRM_000702"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3028376.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQTDEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(15590..16327)
                     /locus_tag="ACFIRM_000703"
     CDS             complement(15590..16327)
                     /locus_tag="ACFIRM_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC3028377.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(16606..18882)
                     /gene="metE"
                     /locus_tag="ACFIRM_000704"
     CDS             complement(16606..18882)
                     /gene="metE"
                     /locus_tag="ACFIRM_000704"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC3028378.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSIEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNAGQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19018..19896)
                     /gene="metF"
                     /locus_tag="ACFIRM_000705"
     CDS             complement(19018..19896)
                     /gene="metF"
                     /locus_tag="ACFIRM_000705"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC3028379.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLRDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20095..20370
                     /locus_tag="ACFIRM_000706"
     CDS             20095..20370
                     /locus_tag="ACFIRM_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC3028380.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            20370..20495
                     /gene="ykgO"
                     /locus_tag="ACFIRM_000707"
     CDS             20370..20495
                     /gene="ykgO"
                     /locus_tag="ACFIRM_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC3028381.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(20532..20798)
                     /locus_tag="ACFIRM_000708"
     CDS             complement(20532..20798)
                     /locus_tag="ACFIRM_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028382.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKMPSETHFSVSDGIFEKGLR"
     gene            20941..22080
                     /locus_tag="ACFIRM_000709"
     CDS             20941..22080
                     /locus_tag="ACFIRM_000709"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC3028383.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22077..22658
                     /gene="metW"
                     /locus_tag="ACFIRM_000710"
     CDS             22077..22658
                     /gene="metW"
                     /locus_tag="ACFIRM_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC3028384.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            22775..23572
                     /locus_tag="ACFIRM_000711"
                     /pseudo
     CDS             22775..23572
                     /locus_tag="ACFIRM_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            complement(23752..24312)
                     /gene="efp"
                     /locus_tag="ACFIRM_000712"
     CDS             complement(23752..24312)
                     /gene="efp"
                     /locus_tag="ACFIRM_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC3028385.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(24355..25506)
                     /gene="earP"
                     /locus_tag="ACFIRM_000713"
     CDS             complement(24355..25506)
                     /gene="earP"
                     /locus_tag="ACFIRM_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC3028386.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(25525..25803)
                     /locus_tag="ACFIRM_000714"
     CDS             complement(25525..25803)
                     /locus_tag="ACFIRM_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028387.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26154..27095
                     /gene="miaA"
                     /locus_tag="ACFIRM_000715"
     CDS             26154..27095
                     /gene="miaA"
                     /locus_tag="ACFIRM_000715"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC3028388.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27099..27818
                     /gene="tadA"
                     /locus_tag="ACFIRM_000716"
     CDS             27099..27818
                     /gene="tadA"
                     /locus_tag="ACFIRM_000716"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC3028389.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            27823..28311
                     /locus_tag="ACFIRM_000717"
     CDS             27823..28311
                     /locus_tag="ACFIRM_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC3028390.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            28377..29129
                     /gene="rlmB"
                     /locus_tag="ACFIRM_000718"
     CDS             28377..29129
                     /gene="rlmB"
                     /locus_tag="ACFIRM_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC3028391.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCNLPDSTAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29191..30566)
                     /locus_tag="ACFIRM_000719"
                     /pseudo
     CDS             complement(29191..30566)
                     /locus_tag="ACFIRM_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            30946..31821
                     /gene="dapA"
                     /locus_tag="ACFIRM_000720"
     CDS             30946..31821
                     /gene="dapA"
                     /locus_tag="ACFIRM_000720"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC3028392.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAAGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEG
                     GQAKVRTALKASGQL"
     gene            31830..33026
                     /gene="bamC"
                     /locus_tag="ACFIRM_000721"
     CDS             31830..33026
                     /gene="bamC"
                     /locus_tag="ACFIRM_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC3028393.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKDKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33083..34015)
                     /gene="pip"
                     /locus_tag="ACFIRM_000722"
     CDS             complement(33083..34015)
                     /gene="pip"
                     /locus_tag="ACFIRM_000722"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC3028394.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8514 a         9294 c         8512 g         8067 t
ORIGIN      
        1 cactatattc tgactgcctc aaagggcaaa aataatgtga gtatgttgag gctgcctctt
       61 tttgaaatga caaacaaaat gccgtctgaa acccgatttc cggtttcaga cggcatttga
      121 tgttcaatca gttgtcaaac ctgattatgc caaacctttt gctttcaaca cttccagcat
      181 cgtcgtgccc aactcggcag ggctgcgggt gtaggcgata ccggcttttt caaacgcggc
      241 gaatttttct tcggcagtac ctttaccgcc ggagataatc gcaccggcgt ggcccatgcg
      301 tttgccttta ggagcggtaa caccggcgat gtaaccgaca acaggtttgg ttacgttgga
      361 ttggatgtat tcggctgctt cttcttccgc agtaccaccg atttcaccaa tcatgatgat
      421 ggcatcggta tctgggtctt cttggaagag tttcagtgcg tcgatttggt tcataccagg
      481 aatcgggtcg ccgccgatac cgatacaggt tgattgaccc aagcccaggt tggtggtttg
      541 tgccacggct tcgtaagtca atgtaccgga acgggaaata ataccgatgc ggcccggagt
      601 gtggatgtgg cccggcataa tgccgatttt gcactcgccc ggagtaatca cgcccgggca
      661 gttagggccg accaagcggg taccgttgcc gttggtttcc aagtaacgtt tggctttgag
      721 catgtccaga gttggaacgc cttcggtaat cacaacgacc aagcctacgc ctgaatcaac
      781 ggcttcaacg atagaatcca acacaaacgg agcgggaacg taaatcacgg atgcgtcagc
      841 gccggtttct ttaacggctt ctttcatggt gttgaataca ggcaggtcta ggtgggtttg
      901 accgcctttg cctggggtaa cgccgccgac aactttagtg ccgtaagcca gagcttgttc
      961 ggagtggaaa gtaccgtttt taccggtaaa accttgaacc aatactttgg tgtctttatt
     1021 aatcaatacg ctcattcttt tctccttagg cgtttacggc tgcaacaatt ttttcggctg
     1081 cgtcattcag accgtctgca gaagtcagtt tcagacctga ttcgttcagg attttcgcgc
     1141 cgagttcggc gttgttgcct tccaaacgaa caacgacagg aacgttgacg ttgatttctt
     1201 taacggctgc cacgatggct tccgcaatca tgtcgcaacg tacgataccg ccgaagatgt
     1261 tgatcaatac gcctttaacg gatttgtctt ccaaaatcag tttgaacgct tcaaccacgc
     1321 ggtctttggt tgcgccgccg ccaacgtcca agaagttggc aggttggccg cctttgagtt
     1381 tgatgatgtc catggtggcc atcgccaaac cggcaccgtt aaccatacag ccgatattac
     1441 cttccagggc aacatagttc aggtcgaatt cagaagcttt cagttcacgt tcgttttctt
     1501 gagatttgtc gcgcaattca gcgatttttg gcaggcggta gagcgcgttg ctgtcgatgc
     1561 cgattttgcc gtccacgcaa gccagtgcgc cgttttcgcg aactgccagc gggttgactt
     1621 caaacagggc gaagtcgttt tcgacaaacg ctttgtacgc gccggtcatc agtttggcga
     1681 actcgttgat ttgtttgtct ttcaagccca gttggaaggc aacttcgcga gcttggcaag
     1741 gttgcaggcc gaccagcgga tcaacggtta ctttgaagat tttttctgga gtttcagcag
     1801 caactttttc gatttccacg ccgccttcgg tagaagccat gaatgtaacg cggcgggtag
     1861 aacggtcaac cactgcgccc aagtacagct cggtttgaac agggtacatg tcttcgcaaa
     1921 ccaaaacgct gttgacaggt tgaccgttgg catcggtttg gtaagttacc aagttggtgc
     1981 caatcaggct ttcagccact tctttggctt tttcgcggct tttaacgact tttacgccgc
     2041 ccgctttacc gcggccgccg gcgtgtacct gtgctttgac aacagcgaat ttgccgccca
     2101 atttgtcgta agctgcagcg gcttcttcgc cgttgtgcgc caaaataccg ccttgtacgg
     2161 gcaaaccgta gctagccagc agttctttag cctgatactc gtgtaaattc atggatttct
     2221 ccttaaggtg agtgggtttt aggaatcgcc ctgcggccaa tcccatattt tcagacggtc
     2281 ctactattta ctgaagggca accacatccg cgccagcaga tacatgactg caccgcctcc
     2341 cccgagaata aaaaagacga tgcctttttt acgcgaattc ggacagagca gataaacggc
     2401 aaggctgaaa ctgataaaca acaatgccac gcgccaaacc aattctttat ctttgaaacg
     2461 gttgagcgta taagtttcgg tcatcatcac atacatctgc cacaatgcgg ccatacacat
     2521 gctgatgatg cccatcggta taaagaaaat accgactatt tctttgttca taatgaatgt
     2581 aaatgctgtt tgtctgacgg aattataaat cagaaaacga agctctcgga acattcaatt
     2641 tgccggaggc cttaaggccg tctgaaaatt tcagacggcc ttaatgtcta tcaaccgtgc
     2701 aaagcgcgtt tgtcggccgc caatgcagct tcgtgaacca cttcggacaa ggttgggtgg
     2761 gcatggataa tacgggcgat atcttcgctg ctggcgaaga attcgagcgc agtcacgcct
     2821 tcggtaacca attcgctgac aaccgggccg atcatgtgta cgcccaagat acggtcggtt
     2881 ttggcatctg ccaacacttt aaccgtacct ttggctttgc ccattgccaa tgcacggccg
     2941 tttgcaccga aacctgaagt accttttttg tactccacgc cttcggcttt gagctgctct
     3001 tcggttttac ccacccaagc gatttcaggg tcggtgtaaa tcacaaacgg tacgttgttg
     3061 aagtcgatat gcggtttttg accggcaatg cgctcggcaa cggcaacgcc ttcgtcgctg
     3121 gctttgtgtg ccaacatcgg gccgcgaacc acgtcgccga ttgcccatac gttaggcagg
     3181 ttggtacggc attcgccatc cactttgata aagccgcgct cgtctttttc caagcctacg
     3241 gcttccgcgt tcaggccttt ggtgtttgga atacggccga tggcaacgat cagtttgtcg
     3301 aatacttcgg ttttggcttc gccggcagca gtttcgtaag caacggaaac acctttgcct
     3361 tcagacttga tgtcgccgat tttcacaccc aattcgatgc tcagaccttg ctctttggtg
     3421 aagtatttga aggcttcttt ggcgatttgt tggtcggcgg cagccaggaa ggtcggcgcg
     3481 gcttcaagaa tggtaacttc cgcacccacg cggttccata cggaacccat ttccaaacca
     3541 atcacaccgg aaccgattac gccgagtttg gcaggtactt cggtcaggtt caatgcgcct
     3601 tcgttgtcca atacgttcac gttgtcgata gcgacttgcg gcaacggacg cggtacggaa
     3661 ccggttgcta cgatgacgtg tttggcttcg ataacggttt tctcgccttt gttatcgact
     3721 tcaatttggt aagcatcgcc atttttaccg gcaaaggaag cagtaccgaa caggctggtt
     3781 actttgtttt tttggaacag gaatttcacg ccgccggtca gtttggtcac gatggcatct
     3841 ttgcgctcaa tcattttggc cgcgtcaaat tttacgtcgc cgacagtgat accgtgttcg
     3901 gcaaaatcgt gttgcgcagc gtggaaatgt tcgctggatt gcaacagggc tttagaaggg
     3961 atacagccta cgttcaagca agtaccgccc aatgcagggg cattgcctgc tttgttaacg
     4021 cctgcatcga cacaggcagt tttgaaaccc agttgtgcgg cacggatggc ggcaacgtat
     4081 ccgcccggac ctgcaccaat cactactaca tcatattgag acatcgtgtc atcctttgct
     4141 tttagcagta tctcaaaata aaaacgatac ttttcgtttc tttttttatt ttgaatgact
     4201 gcgttttgat ggttttaaga aaaagccgtc tgaaagtcca tatctgccga ttcattcgaa
     4261 tcaaatgttt cagacggcct caatcgttcg ttgagcctat tgtagcacga tactacatta
     4321 cgctacatct aaataaaaat tcagacggcc ttttcattaa caaataaaaa gccgtctgaa
     4381 acgattacag atccaacaac aggcggaccg ggtcttccaa cgcgtcttta atggctacca
     4441 aggtcaatac agcttcgcgg ccgtcaatga tacggtggtc gtaagacaga gccagataca
     4501 tcattggacg gacaacaact tggccgtttt caaccacagc gcgctcttta gtggcatgca
     4561 tacccaaaat cgcagattga ggcgggttga tgatcggggt ggacatcata gaaccgaaag
     4621 taccgccgtt ggtaatactg aatgtaccgc cggtcaggtc ttcgatagcg attttgccgt
     4681 ctttggcttt tttcgcgtaa tcaacaattg cttgttcgat gtcggcaatg ctcatttggt
     4741 cggcatcgcg caggattggt acaaccaaac cgcgtgggct gccaattgcg ataccgatgt
     4801 cgaagtagcc gtggtacaca atgtctttgc cgtcaacaga agcattcaca accgggtatt
     4861 ttttcagggc tgtaacagcg gctttaacga agaaggacat aaagcccagt tttacgccat
     4921 gttctttctc gaatttttct ttgtacttcg cacgcaagtc catgatcggt ttcatgttga
     4981 cttcgttgaa tgtagtcaga atggcgtttt cttgttgaga agccaagagg cgttctgcaa
     5041 cacgggcacg caggcggctc attggtacgc gttcttcagg acgtgcgccg gcaggaagtg
     5101 caacggcagg ggccacggct gcggcaggtt tggcagcggc attttgtacg tcttctttca
     5161 atacgcgacc gtcacggccg gaaccttgca atacgttcac gtcaacaccg gtctcggcag
     5221 ccagttttgc agcggcaggc atggcggcgt tgttttgtgc agcagcagga acggcagccg
     5281 gggcagcttc ggcaggagcg gctgcaggtg cttcagcagc gacagtagca gctgtatcga
     5341 tacgggccaa aacttggtcg gcaacaacgg tttcaccgtc ttgcgctacg atttcaacca
     5401 atacgccggc ttgtggagaa ggtacttcca aaaccacttt gtccgtttcg atatcgatca
     5461 ggatttcgtc acgggcaact gcttcgccaa cttttttctt ccattccaag agtgtgcctt
     5521 cagatacgct ttcagacagc ataggtactt ttacatcaat aatcattttg tgtctccaat
     5581 ggccctttca gacggcctgt tgtgtgtttt taattctgca tatgggcatt ccgcctgagc
     5641 agactcagac ggaatgctac ttggtttata acgccaaagc gtcttcaacc aattgtttca
     5701 attgagcaat gtgtttgctt gagtagccca ctgcaggcga tgcgctgctt ggacggccgg
     5761 cataagacag tttttgctct tcgctgataa cgtcttcgat gcggtggcgg atttggtaga
     5821 acgcgccttg gtttttcggc tcttcttgcg cccaaaccac agattttgcg ttcggatatt
     5881 tcgccagttc ggctttaacc tcgtcgtatg ggaacggata cagctgctca acgcggacga
     5941 tagcgacatc atcttccagt ttacgttcgg ctcgaccggc ttccaagtca tagtaaacct
     6001 gaccggcaca caataccacg cgtttcacgc tgtcgttgct tgcgcgttcg gcggtatcac
     6061 caataaccgg acggaaagtc gaaccttcgg tgaagttttc cagcgggctc attgcacctt
     6121 tgaagcgcaa caggcgtttg gacatgaaaa tcaccagcgg tttgcggtat gaacccaaga
     6181 cttggcgttg caagagatgg aacatttgcg acgcttcaga cggcatgatg acttgcatat
     6241 tgttctcaga acacagttgc aaccaacgtt ctacgcgtgc agaagagtgt tcagggcctt
     6301 gaccgtcgta gccgtgcggc aggatggtgg tcagaccgca caagcgaccc cacttggttt
     6361 cgcctgaaga caggaattga tcaatagtta cttgcgcgcc gttggcgaag tcaccgaatt
     6421 gagcttccca aatggtcagc ttgtcaggag cggagcaggc aaagccgtac tcgaacgcca
     6481 tcacggcttc ttcgttcaag atagagtcga taaccaggaa ctcgcccaag ccttcgccca
     6541 tgttgcgaag aggaacataa gtaccgtcgt cccatttttc gcgtttttga tcgtgcagaa
     6601 cggcatgacg gtgtgagaat gtaccgcgac cggagtcctc accggagata cgcacgccat
     6661 gacctttggt cagcaggctt gcgtatgcga gggtttcggc cataccccag tcgatggctt
     6721 gtttgccgga tgccatggct ttacgcgctt cgatcacacg ttttgcagtc ggatgcaggg
     6781 caaagccttc cggcacagcg gtgaatttct cagtaagacg ctcaatgtcg gcggcaggca
     6841 aaccggtttc gattttttcg cgccaatctt taccttggta tttgctccag tcgatttgtg
     6901 tgcgttggaa gttgctcaac gttgtttgct caacatgttc gcctttgtcc aaagcatcac
     6961 ggtaggcttg gatgtaaccg tcggcctcaa cttgagttac cacgccttcg gcaatcagtt
     7021 gctcggtgta caaagcacgc gcacccggat gttgcgatac ttttttgtac atcatcggtt
     7081 gggtcaaagt cggatcatcg ccctcgttgt gaccccattt acggtagcag acaacgtcga
     7141 ttacgatgtc tttatggaat tttttgcggt aatccaaagc ggcttgaatg gcaaagcaaa
     7201 cgcgttcggg atcatcgccg ttcacatgga taaccggagc ggaaaccatt tttgcgatat
     7261 cggtacagtg tacggttgaa cgggtatcgc ggatatcgga agtggtaaag ccgatttggt
     7321 tgttgatgac gatatggacc gtaccgcctg tggtataacc gcgcgtttta gacaggttga
     7381 atgttgcttg gttgacaccc aaaccgataa atgcggaatc gccgtgaatc aataccggca
     7441 agactttatc gcggccgttt tcgcccaaac gtttttgttt ggcgcgtgca gaaccttcca
     7501 ctaccggatt gacgatttcc aagtgtgacg ggttgaacgc caaagaaacg tgcatcgggc
     7561 cgtgaggggt ggcaatatcg gagctgaagc ccatatggta tttcacgtcg ccactaggca
     7621 gtttgatttc ggcacggcct tcaaattcgg caaacaaatc gccgggtttt ttgcccaaaa
     7681 tgttcaccaa aacattcaga cggccacggt gcgccatacc gatgatgact tcttccacgc
     7741 cgtccttacc ggcgttttga atcaggtagt tcaaaccggc aatcgcgctt tcgccgcctt
     7801 cgacaccgaa acgtttctga ccgacatatt tggtatgcag ataacgttcc aaagtctcgg
     7861 cagcggtcat ctctttcaag atacggcgtt tttgatcggc attgtaatgc ggtgtggaca
     7921 atacgctttc aaaatagttg cgtacccaac ggcgctcttc ggtattggga atatagatat
     7981 attccaatgc gatgtggccg cagtaggttt gtttgaggtt gctgatgatt tgggacaagg
     8041 gcagtttgcc gcgattggca aaatcaccct cgcccatatt gaatcgaagc gccatatcgg
     8101 catctgacag accgtggaat ttcggatcga gggcttcaat atcgcgcgga gggatacgtt
     8161 tgagcggatc aagttgggcc gcacccacgc cttggatacg ataggcagaa atcagccgta
     8221 aaacgctgac ttgctttttc agcattgcct catccgcacc gcccgcaacg gcagatgcaa
     8281 ttttcttttt cgccaaagta acaaatgatt cgcgaatcgg tgtgtgtgcg acatcgacag
     8341 caaccgtccc cggctgtttg ctcaaatcgg tgaaatactg cttccatttt tcatcaaccg
     8401 catcggggtt ttccaaaaaa gcctcgtaca attcctcaat gtagggtgcg ttcgaaccga
     8461 acagataaga gaaattgagt ttttcgtcca tcatggcgtg cgctcttttc tttaaaatat
     8521 aaacaaaggg tagaagtccg cgtcctgacg gctgaactga tgccgtctga aaagacattt
     8581 tattctaccc tattttaaac cggattgctt gtcttgattt catccgacta caatcagacg
     8641 gattcaggaa acaatccggt tctctgatat tgcctgtttg acaatatatt aatgtatgtt
     8701 acctctcgcc tgccggcaca taatcgcggc gttccgaacc ggtataaagc tggcgcgggc
     8761 ggccgatttt cagcgaagga tcgctaatca tctcgtgcca gtgcgaaatc cagcccacgc
     8821 tgcgcgacag ggcgaagatg acggtaaaca tttcggtcgg gatgcccagc gcggacagga
     8881 cgatgccgga atagaaatcg acattgggat acagtttgcg ttcgataaag aacgggtctt
     8941 tcagcgcaat ctgttccaat tccatcgcca gtttgaattt cggactgtct tccaagccca
     9001 attccttcaa aacttcatag caggtttcgc gcataatgct ggcacgcgga tccatattgc
     9061 ggtatacccg gtgtccgaag cccatcaggc ggtatttgcg ctgtttcacg ccttccatgt
     9121 atgcggcaac attggacaca tcgccgattt catccagcat tttcaacacg gcttcgttcg
     9181 caccgccgtg cgaagcaccc cacaggcagg cgataccggc agcaatacag gcaaacgggt
     9241 tcgcacccga agagcctgcc agacggacgg ttgaagttga ggcgttttgc tcgtggtcgg
     9301 catgcaaaat aaagatgcgg tcgagcgcgc gtgccaaaac gggattaggt ttgtagtctt
     9361 cacacggtgt ggcaaacatc gtatgaagga agttttctga ataagaaaga ttattcttcg
     9421 gataattgaa cggcagaccg tttgaatagc ggtaacacat tgccgcaatg gtcgggattt
     9481 tggaaataag gcggtaaatc gcgattttgc ggtgttcggg attagtaatg tccaagctgt
     9541 cttggtagaa cgcagacagc gcgccgacca cgccgaccat catcgccatc ggatgcgcgt
     9601 cgcggcggaa accccggaag aaccaagtca gctgttcatg caccatcgtg tggcggcgga
     9661 ctgtattgtc aaattctgcc ttttgctcgg gagtcggcag ttcgccgtaa atcaacaggt
     9721 agcagacttc caaataatcg gacttttcgg ccagctgctc gatggggtat ccgcgataat
     9781 aaagcaagcc ttgatcgccg tcgatgtaag taattttaga ctcacagctt gcggttgaaa
     9841 caaatccggg gtcgaaggca aacaaacctg tattttttgt cagcccccga atgtcaacca
     9901 catcatgccc gatgctggct tccaataccg gcagctccaa acctgcccga cccggtacgt
     9961 tgagtttgat tgatttggac atattatcgc tcctttgcaa gttataaccc acgttactgc
    10021 atccgcagca cgttctttgc tttcagacgg cctttgcatt ttgaaatctg ccctcagaca
    10081 aatttcagat gcctcatgcc cgtctgattt tttcaagcat cgggataagg tgccctttgt
    10141 ccgtttccga atgcccgtga atcaaggcaa gcaattcttg atcttgaaat tcaaggattt
    10201 cggaaaactc ggacagctct ttatcgctca aatgctcgaa ttctttttcc ataaacctgc
    10261 cgaagattaa atctagttcc aacaatcccc ggcgggtttg aaaacggatt ttccgtttgg
    10321 caatgtcgtc aaaaaccatc atttcttaaa caacccgttt caacataatc tctttaatct
    10381 taccgatggc ccgggtcgga ttcaagtgtt tggggcatac gtccacgcag ttcataatgg
    10441 tgtggcagcg gaacaaacgg tacggatcgt tcaaattatc caaacgctca ttagtgatgg
    10501 tatctcggct gtccgcaatg aagcggtagg cgttcagcaa gccggacgga ccgacgaatt
    10561 tgtcgggatt ccaccagaat gacggacagg cggtcgaaca gcaggcgcac aaaatacatt
    10621 cgtacaaacc atccaactct ttgcgctctt cctgagtttg cagacgctct ttgtccgcat
    10681 caatcggatt gtcgttaaca acataaggtt tgacggaatg gtattgtttg aagaactggg
    10741 tcatatccac aatcaggtcg cggatgacgg gcagaccggg cagcgggcgg attttgacag
    10801 gctgtttcaa gctgcgtaaa tcggtcagac acgccaagcc gtttttgccg ttgatgttca
    10861 taccgtccga tccgcaaatc ccttcgcggc aggagcggcg gaaagacaag gtatcgtctt
    10921 gtgctttcag gcgtaccaaa gcgtccaaaa gtttcacgtc ggtcggttcc aattccaact
    10981 cgtaacgctg catataaggc ttggcatcaa cgtccgggtt gtaacggtaa atttcaaaac
    11041 tcattttttc catgagaagt gttccttttc tcatcaactg cccaatgatt ttgtattata
    11101 aattcaagca gttcaatgtg ggtatggtta ggtttcagac gacctttgag gcgaagaccg
    11161 tctgaaaacg catcaataaa cgcgtttggc cggtttgatg tattccacgc tcaaaggctt
    11221 ggtgtgtacc ggtttgtaag acaaggtatt ggtgtccgaa tgatacaggg tgtgtttcat
    11281 ccaattttcg tcatcgcgct caggatggtc gtctgaagcg tgcgcaccgc gtgattcttt
    11341 acgtgcttcg gcagacacca aagtcgcttt cgccacttca atcaggttat ccaattccaa
    11401 agcttcgata cgcgcggtat tccacacttt gctcttgtct ttgatttcgg tacgtttcac
    11461 acgctcggca atcgccatga tttcttgaac gcctttgctc agaatctcat cggtacggaa
    11521 cacgccggcg tgcagttgta cggagcgttg cagttcgcga cgcaatgcat caacgttttc
    11581 accatcggtt tgactgtcca aacgctcgat acgttggcgg gtcaactcac cggcattagc
    11641 aggcagaggt ttccaatcgc tttgctcttt gatgaattta atcatgctgt caccggcagc
    11701 tttgccgaac accaccaagt ccagcaggga gttcgtaccc aaacggttcg caccgtgtac
    11761 ggaagcacag gcgcactcac ctgcggcata caggcctttt acaggtactt cgtactcgtc
    11821 gccttgcgga acaacaactt caccgtgata attggtcgga atgccgccca tcatatagtg
    11881 ggtagtcggc acaaccggaa tcgggtcttt aatcggatcg ataccggcaa actgaatgga
    11941 aatctcgcgg atgcccggca gtttttccat aattttttct gcaccgatat ggtcgatttt
    12001 cagtaagacg tggtctttgt ttttaccaca gccgcgacct tcatagattt ccatcgccat
    12061 cgcgcgtgaa accacgtcgc gagaagccaa gtcttttacg gtcggcgcat agcgttccat
    12121 aaagcgttcg ccgtcggcgt tcaacagaat accgccctcg ccgcgtacgc cttcggtaat
    12181 caacacgccc gcacccgcca cgccggtcgg gtggaattgc cagaattcca tatcttccaa
    12241 cggaatgccc gcacgggcgc aaatgcccaa accgtcaccg gtattcatat aagcattggt
    12301 agaagaagca taaatacgac cgccgccacc ggtagcaaac atcacggcct tggcgtggaa
    12361 aatataaact tcgcccgttt ccatttccat ggcggttacg ccgacgacat cgccgttttc
    12421 atcacgaatc aaatcttgcg ccgtccattc cacaaagaat tgtgtattgg cacggacgtt
    12481 ttgttggtac aaagtatgca acatcgcatg accggtacgg tcggcaaccg cacatgcacg
    12541 ttctaccgca cgtttaccat gttcggcagt atgtccgccg aaaggacgct gataaatttt
    12601 gccgctttca acgcggtcaa aaggcatacc catgtgttcc aactcaatca ccgcttcagg
    12661 cgcagcgcga cacataaact caatcgcatc ttggtcgccc agccagtcgg aacctttcac
    12721 ggtatcgtac atgtgccagt cccaacggtc ctcctgcaca ttacccagag aggcggaaat
    12781 accgccctgc gccgctacgg tatgcgagcg ggtcgggaac actttagaca aaacggcaca
    12841 attcaaaccg gatttggata attggagggc tgcacgtaaa cctgcaccgc caccgccgac
    12901 aatcacggca tcaaacttgc gaacaggaaa acccatactt acccccaaat cactttaact
    12961 gaatacacga ggcagccgac cagccagaca atggtggcaa cctgcaaaaa caaacgcacg
    13021 ccgaagggtt tgatatagtc catccacaaa tcgcggatac ccacccaagc gtgcaagaat
    13081 acggcgataa agctcacttg ggtaaatact tttacccaag cttgactaaa aaatgcctgc
    13141 catgccggat attctttagg cagggcaaat agaaccacta aaagtgcaac ggtataaatc
    13201 aacataataa ccgcagtcgc acgctgcatt acccaatcgc gcaaaccgta atgggcaccg
    13261 gtcaatttac gttctaccat aacaacgctc ccaaaacgac agtcaaaacc aatgcagaag
    13321 caaatacggc tttagcggta ttgcgcgcag tattcagctc aaggcctttg tgcgcatcca
    13381 aaaataaaaa gcggataccg gcgagagaat ggtgcagata agcccacaat ataccgatta
    13441 aaaccagctt gaccaaagga tgggaaacaa tggcacggta agtttcaaat gcagactctt
    13501 gactcagggt accggacaga aaatacagca ggaaaggcag cataataaac agcccgaccc
    13561 cgctaatgcg gtgaaggatg gaaactatcc cgggtatcgg cagacggatg ttcggcaaat
    13621 ccagataaac aggacgcagt ttgacagaca tagtcaattc ctctgtagtt atatatcgac
    13681 tcaaaaaccg taaacccatt gctacactgg gttacatcaa cgttaattta cctgttttat
    13741 cccattttga acagtgtatt ttaaaaacaa actgatagat tttgacaaac acgcgtggaa
    13801 acagcagatt aaaagaaaga tttttagttt tgccgctccc ttcccgacac ggtatcagca
    13861 tccgaccccc aaccccatcc ccaaaaataa aatgccgtct gaaaaaaatt tcaggcggca
    13921 ttgttttgcc atatcggtca ggctttcaat ttcgcatcgg catcgcgcaa cgcggtacgc
    13981 agaccttcct cgataacagg atggtagaac ggcatatcca gcatttgcgg aacggtcatc
    14041 ttcatttgat gcgcccatgc caacaagtga gccaaatgtt cggcggcagg gcctacgatt
    14101 tccgcgccga taaagcggcc ggtggctttt tcggcataca ggcgcatatg gcctttgttt
    14161 accagcatca cgcggctgcg accttgattt ttgaacgata cttcgccgat gacaaattcg
    14221 tcggtttggt attgcgcggc aacctgcgcg tatttcaaac cgacaaagcc gatttgcgga
    14281 ctggtaaaca ccacgccaat ggtgctgcgg cgcaaaccgc tgccgatatt cgggtagcgg
    14341 cccgcgttat cgccggcaat cttaccttgg tcggcggctt catgcagcag gggcagttgg
    14401 ttggacgcgt cgcccgcaat aaagatatgc ggaatgctgg tctgcatggt cagcggatcg
    14461 gcaacgggta cgccgcgcgc gtctttgtcg atattgatgt tttccaaacc gatattgtca
    14521 acgttcggac ggcgacctac ggctgccaac atatattcgg caacaaatac gcctttttcg
    14581 ccatcctgct cccaatggac ttctacattg ccgtctgcgt cgagtttgac ctcggtttta
    14641 gcatccagat gcagtttcaa ttcttctccg aacacggctt tcgcctcgtc tgaaacaacg
    14701 gggtcggaaa tgccgccgat gattccgccc aaaccgaaaa tttcaacttt cacacccaaa
    14761 cggtgcaatg cctgacccag ttccaaaccg ataacgcccg gcccgaacac ggcaacgcgc
    14821 ttaggcagcg tatcccacga gaaaacgtcg tcgttgataa tcaaacggtc gcccaaagac
    14881 tgccactgcg gcaggatgac gggacgcgaa ccggtagcaa tcacgaaact ttttgccgca
    14941 atttggatat ggtcgtcgat ttggacggta tgctcgtcga tgaatttagc cgagcccatg
    15001 atgcgcttgt cggcaggcca ttcttccaca tcggtaacga caaagccgac aaaacggtcg
    15061 cgctcggatt tgacgcgccg cataacctct tctccgttga cgacgatgct gtctttgtcc
    15121 aaatgcacgc cgaaggggtc ggtatgcaat gcgtggtgac gcgcttctgc ggcggcaatc
    15181 aagagtttgg aaggcataca gcccacgcgc gcgcaggttg tgccgaacac attgttttca
    15241 atcaggtaaa cattatccga atgtaaacgg gcattgcgaa acgcacccat accggcagta
    15301 ccgccgccga ttacgacgat atccgcttga atttttttca taatctttgt ccttccgttt
    15361 tcgtctgaaa cattgccgac acagaacata tcggtttcag acagcattta aatatcttcc
    15421 caatcaaaaa cattattcag ataaataaaa acaaaatttc gattgagtgc gacaatatcc
    15481 aaatcttctt gtaaatatag agttattcgg agcggcagta tagaaccgcc cgaataactc
    15541 tatccggtat atcagacagt ttaatcaaac tgccttaagc aaacagctat cagtttttag
    15601 ccaagtaagc ttccaaatct tcgctgccgc cgatgtattt gccgccgatg aagacttgag
    15661 gggcagtcat cttgccggta atagcgcgaa cggaagtaac ggttgcatct ttgcccaata
    15721 cgatttcttc gtaagacaaa cctttgtctt gcaaagcttg tttgactttg gcacagaatt
    15781 ggcaacctgg tttagtgaaa attgccacag actcttgagc cttccaatcg ggagcaacga
    15841 atttcagcat agtatctgca tcggatactt tgaaaggatc gcccggttct tcaggttcga
    15901 tgaacatttt ttcaaccacg ccgtcgttaa ccagcatgga gtaacgccaa gagcgtttgc
    15961 cgaagcccaa gtcttcttta ccgaccagca tacccatacc ttcggtaaat tcgccgttgc
    16021 cgtcaggaat catgtagatg ttgtctgatt cttcttcggc agcccaagcg ttcattacga
    16081 aagtatcgtt tacagatacg cagcagattg cgtcaacgcc gttttctttg aacgcgccga
    16141 acaattcgtt gtaacgcggc aggtgtgaag aagaacaagt cggggtaaat gcaccgggca
    16201 gggagaatac gactactttt ttgcctttga acaaatcatc ggtagacaca tctttccaag
    16261 tgtcgccgac gcgggtgcgg aatactacgg aaggtacttt ttgaccggta cgatcttgta
    16321 aagccatttt gagctcctta atagattggt ttgagaaata attgtgaatc gccgcgcatt
    16381 ctaccccatg caaaatgatt tgtataattt atagtttaaa taatataaat tatttaaaac
    16441 tatcaaatac aactccgatt tgcctgttcc caacatataa agccgaatta gagaaaaaca
    16501 agctgcaatg aaaagatttt ccggcaactg ctcccctgcg ccgcaactca ggacaaaatg
    16561 ccgtctgaaa ggctttcaga cggcatttgt tcatacggtc tcggcttatt tcgccaattc
    16621 ggcacgcagt ttgcgggtta cgttcatcat tacttgaagc tgttccagag tttctttcca
    16681 gccgcgtgtt ttcaggccgc agtccgggtt aacccacaga cgctcaaccg gaacaacctc
    16741 aatggctttg cgcaacaggt gttccacttc ggcttctgtc ggtacgcgcg ggctgtggat
    16801 gtcgtaaacg cccgggccga tgtcgttcgg gtatttgaat tcgccgaacg cagccaagag
    16861 ttccatgtcg gaacgtgaag tctcaatggt gatcacgtcc gcatccattg cagcaatcgc
    16921 aggcaggatg tcgttgaact cagagtagca catatgagtg tggatttgag tgctgtcttc
    16981 gcaacctgca gaggataggc ggaaggattc gcccgcccag ttcaggtagg catcccaatc
    17041 ggcacgtttc aaaggaagac cttcgcggat ggcaggttcg tcaatttgga tgactttaat
    17101 gccggctttt tccagatcca atacttcgtc gttcagagcc agtgcgattt gtttgcacac
    17161 ggtagcgcgc ggaatatcgt tgcggacgaa agaccattgc agaatggtta caggaccggt
    17221 caacatacct ttcatcgggc gtttggtcag gttttgtgcg taagtagacc aagcgacagt
    17281 catagcttca ggacggctta cgtcaccgaa gatgataggt ggtttaacgc agcgtgagcc
    17341 gtagctttgc acccagccgt attgggtgaa tgcaaaaccg ctcaacagtt cgccgaagta
    17401 ttcgaccatg tcgttacgct cggcttcgcc gtgcaccagt acgtccaagt ccagtttttc
    17461 ttgctcttca accaccaagg cgatttcttt tttcattgcg gcttcgtaat cggaggcaga
    17521 cagttcgcct tttttgaagg ctgcgcgtgc ttggcggatt tcggtagttt gcgggaaaga
    17581 accaatgttg gtcgtcggca gcagaggcag gttcaaccat gcttgttgcg ctttgatacg
    17641 gtcggcaaac ggtgatttgc gttggcctgc gttgacaggc aggtcggcca agcgtttggc
    17701 aacgtctgca cggtggattt cactgctgtt ggcacgggag tcggcagcag cttggctggc
    17761 ggccagttct tcggcaacag aatcacggcc ttcattcaat gcggctttca gaacacgcaa
    17821 ttcttgggtt ttttgcagag tgaatgccaa ccaagagtac aggtcgggtt tgttggcttt
    17881 cagtttttct tcgattgaca agtcaaacgg agtgtgcagc agcgagcaag agctggaaat
    17941 ccacaaacgc tcgcccagtt tggcttgcag aggcccgacg gtttccaaaa ctttgttcag
    18001 gttggcgcgc caaatgttgc ggccgtcaat cacgccggca gacaggactt tgtcgtagcc
    18061 ggcgaacgcg tccagttgct cgggtgcgcg taccaagtcg atgtgcaggc cgtcaacagg
    18121 cagggatttc aacaatgcgg cgtgttcggc aacagaaccg aagtaagtgc ccaacaggat
    18181 tttggcgctt actttgttca aagtagcgta aacgtctttg tatgcttcta cccattcttt
    18241 aggcagatcg acagccaaag caggctcgtc gatttgaatc cactcggcac cggcttcaac
    18301 caaagcagtc aggatttcaa cgtaaacagg caacagttta ggcaacaggc tcagacggtc
    18361 gaattcaacg gagccttttt ctttacccac ccacaggaaa gtcaaaggac cgacaacggt
    18421 cggtttggct ttcaagccca aagtttgggc ttcttgcagt tgttgaacgt agtgtttggc
    18481 gttggctttg aattcggtat cggcatggaa ttcaggcacc aggtagtggt agttggtgtc
    18541 gaaccatttg gtcatttcga tggcgaattg gtctttgttg ccgcgcgcca gttggaagaa
    18601 ttgttccaaa ggcaggtttt ggctatcgaa gccgaaacgg gcgggaatcg cgccggtggc
    18661 aacttgcagg tcgaggatgt gatcgtagaa agtgaaatca cctacagcaa cgtaatcagc
    18721 gttggcagca gcttggtgtt tccagttttt ctcgcgcagg tctttggcaa cgtcgagcag
    18781 ctcttgttca ctgatctctt tgcgccagta tttttcttgg gcgaatttca attcacggaa
    18841 ggcgccgaca cgcgggaagc ctgagaaatg taatgttgtc atgttgaact ctccagttgg
    18901 attttgtaag gaccggttca atccgaatcg gccggtattg taaatcagaa tggctcggat
    18961 agggcggcgt tttgcttttc agacggcctc ggatgctgtc tgaagccgtt ttcggcttta
    19021 agggcgcgcg cctaaaatat ggcagatggc gtaagtcagc tcgctgcggt tgagcgtgta
    19081 gaagtggaaa tctttcacgc cttcgcggga caggacttta accatatcga tggcgatact
    19141 ggcggcgacg aggttgcgcg tgccttggtc gtcgtccaag ccttcataca tttgcgacag
    19201 ccatttcggg attttgacgt tggttacttg tgccattttg ccgagttgct tgaagttggt
    19261 aacaggcagg atgccgggaa cgatttcgac gtcgatgccc aacatcacgc agcggtcgcg
    19321 gaagcgcagg tagcgttcca catcgaagaa gaattgggtg atgacgtggt tcgcgcccgc
    19381 atcgattttg cgcttcagat taatcaggtc ggcttgtgcg gatttcgctt cgggatgtac
    19441 ttcggggtat gctgctacag agatgtcgaa gtcggcgacg gagcgtaata gcttaaccaa
    19501 gtcttcggcg taaaacggtt ttttctcata accggccggc tcgtcgccac gcagggcaac
    19561 gatgcggcgg ataccgctgt cccaatagtc tttggcgatt tggcgcaatt cgtcgtggga
    19621 tgcatcgatg ccggtcaggt gcggagctgc ttccaaaccg gtttcctgtt tgatgcgttt
    19681 gacgatgccg tgcgtgcggt cgcgttcgcc ggagtttgcg ccgtaagtta cggatacgaa
    19741 cttgggatgc agggtttgca gacggtggat ggaatctctc agcatcgttt ccatttgttc
    19801 gtttttcggc gggaaaaatt caaacgaaac gttgatgttg cctttcaaat cggaaaggct
    19861 gttgtttaac gcattgattt cttttgcata attcatatct gcataccttc gttatctttt
    19921 acatgatgtt tcaggcggta tgataaggtt gcaccgaata tgagtcaatt tcaaaatttt
    19981 caggtttatg atgaaaattt ttaatattta gcaaaaacta tacgcttttc ttgtacaagc
    20041 cttctaaatt atgataaagt ttgttatgtt ataacacttc aagcaaggca atatatgaaa
    20101 ccgaatattc atcctgacaa ttatcgaacc gtcctttttt tcgacagcag tgccaatgaa
    20161 ggctggctga ttcgttcttg cgccggaacg cacggaaaaa caatggtttg gacagacggc
    20221 aaagaatatc cgctcttttc cttagatact tcctcttcct cacatcccgt ctataccggc
    20281 aaacagcgta acgtcaatac cgaaggccgt gccagcaagt tcaaccaacg tttccaatcc
    20341 gtcatgtcat cgtttagaaa ggacaaataa tgcaggttct gtcttcgctc aaaaccgcca
    20401 aacaacgcca ccgcgactgc caaatcgtca gacgcagggg caaagtgtac gtcatctgca
    20461 aaagcaatcc gcgttttaaa tcgcgccaac gctgaccccc tgcccttcct gcttcggttg
    20521 taaagcccct cctaccgcaa gcccttttca aatatgccgt ctgaaacaga aaaatgcgtt
    20581 tcagacggca ttttccgtcg atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
    20641 taccaaaaca gcgtcgcggc ggctgcaccg agcagtaccc atgcgctgat ttcggcgact
    20701 tcgcgcagga cacgtttctg cctgcggcta aagaggcggt tgataatcag cgcggcaagt
    20761 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagcga aaagtatggt
    20821 tcgggcgcgg cggcatggga aaggatgttt cagcgcgtca ggctgcttgt ccgaccgtgt
    20881 caaaaagtca tgccgtttgc gatataataa cgcttttcgc cgcatttgtt ggaacaggat
    20941 atgagtcaaa atacctcggt gggcattgta acgccccaaa aaattccgtt tgaaatgccg
    21001 ctggttttgg aaaacggtaa aactttgccg cgtttcgatc tgatgattga aacctacggc
    21061 gagctgaatg ctgaaaaaaa caatgcggtt ttaatctgcc acgcgctgtc gggcaaccat
    21121 cacgttgcgg gcaggcattc ggcggaggat aaatatacgg gctggtggga caatatggtc
    21181 ggtcccggaa aaccgattga tacggaacgt tttttcgtgg tcgggttgaa caatctgggc
    21241 ggctgcgacg gcagcagcgg gcctttgtcg atcaatcctg aaacgggcag ggaatacggc
    21301 gcggattttc cgatggttac ggtgaaggac tgggtaaaat cacaagccgc gcttgccgat
    21361 tatctcggca tcgaacaatg ggcggcggtt gtcggcggca gcttgggcgg catgcaggct
    21421 ttgcagtggg cgatttccta tcccgaacgt gtgcgccacg ccttggtgat tgcgtctgcg
    21481 ccgaaactgt ccacgcaaaa tatcgcgttt aatgatgtag cacgtcaggc gattttgacc
    21541 gaccccgatt tcaatgaagg acattaccgc agccacaaca ccgttcccgc gcgcggtttg
    21601 cggattgccc gtatgatggg acacattacg tatcttgccg aagacggttt gggcaaaaaa
    21661 ttcggacgcg atttgcgttc caacggctat caatacggct atagcgttga atttgaagta
    21721 gaatcctatc tccgctatca aggcgacaaa ttcgtcgggc ggtttgatgc taatacatat
    21781 ttgctgatga ccaaagcttt ggactatttc gatccggcgg cggatttcgg caacagcctg
    21841 actcgcgccg tgcaggatgt gcaggcaaaa ttctttgtcg ccagcttcag caccgactgg
    21901 cgtttcgcgc ccgaacgttc gcacgaactg gtcaaggcac tgattgccgc ccaaaaatcc
    21961 gtgcagtata tcgaagtcaa gtccgcacac gggcacgatg cctttttaat ggaagacgaa
    22021 gcctatatgc gcgccgtaac ggcttatatg aacaatgttg acaaggattg ccgattatga
    22081 acctgcgcga tgatttgcaa ctgatttacg accggatacc cgaaggcagc cgcgtcttag
    22141 acttgggctg cggcgacggc gaattgctgg cggctttggc ggaacataaa aaatgcagcg
    22201 gctacggcat cgaaatcgac accgacaacg tgattgctgc gatgtcgcgc ggcgtaaatg
    22261 ttatccaagc cgatttggaa gaagggttga ccgcattcaa cgatcaaagt tttgatgtga
    22321 ttgttttgag ccaaaccatc caagcgatgc agaataccga aaaaatcctg cgctgcctga
    22381 tgcgcctcgc caaacaggca atcgtcagtt tcccgaattt cggctactgg cgcaaccgcg
    22441 tccaaatcgc actcggcgga catatgccgg tttccgaacg tatgccctac cattggtacg
    22501 acacgcccaa tatccattgg tgcacgctca aagactttga tttgttgtgc gccaaaaaca
    22561 atatccgcgt cctcgaacgc gccgtgatga cgggcaacag acaggtcaaa catttcccca
    22621 accttttggg cagccttgcg ttttaccgcg tcgggtaaga caggttgaaa accttgtcgg
    22681 caataacggg tttctcggtt ctattaacaa cagtccgtct gaaaaatgaa acggcaggtt
    22741 ttcagacggc cttttcaata ttggaaacaa tagaatgaaa aacaaaacgt catcacttcc
    22801 cttatggctt gccgcaatca tgctggccgc gtgttccccg agcaaagaag ataaaacgaa
    22861 agaaaacggc gcatccgccg cttcgtcttc cgcgtcccaa tccgatttgc aaccggccgc
    22921 atccgcccct gataacgtca agcaggcaga aagcgcgcca ctgtgaaatt gcaccggcct
    22981 gcaccccgcc gccggcattg gcgatctcat gcagcaaatc gccgaacaca tcgactcgga
    23041 ctgtctgttt gccctttccc ataacgaact ggaaacccgt ttcggcttac ccggcggcgg
    23101 ctatgacaac atacagcggc tgctgtttcc cgacatccgc cctgaagatc ccgactacca
    23161 tcagaaaatc atgctggcaa tcgaagactt gcgttacgga acgcgcacta tcagccggca
    23221 ggcacaagat gccataatgg aacaggaacg ccgcctccga gaagcgacgc tgatgctgac
    23281 acagggcagt caagaaagcc gagaacaagg cgaggaaccg aaacgcgcac gttattttga
    23341 agtttcggca acatctgcct atttgaaccg gcacaacaac ggactcggcg gcaatttcca
    23401 atacatcggc caattgcccg gctatctgaa aatacacgga gaaatgcttg aaaaccaatc
    23461 actcttccgg ctgtccaacc gtgaacgcaa tcccgacaaa ccgtttttag acatccattt
    23521 tgacgaaaat ggcaaaatca cgcgtattgt cgtttacgaa aaaaatatct atagtggatt
    23581 aacaaaaatc aggacaaggc gacgaagccg aagacagtac agatagtacg gcaaggcgag
    23641 gcaacgctgt actggtttaa atttaatcca ctatacttca atccaaacac ggggcgaata
    23701 taaaaaaatg ccgtctgaaa cgttttcaga cggcattttt caagtaggat atcaggcgcg
    23761 tttgcggaat tcgccggtac gggtatcgat ttcgactttg tcgccgtttt cgatgtaaga
    23821 catcacttgg atttcggtgc cgccgaccag acgcgcggtt ttcatcactt tgccggaagt
    23881 atcgcctttg acggcaggct cggtgtactc gacttcgcgc acgatgatgg tgggcagttc
    23941 gacagaaatg ggattgcctt catagaaggt aacttcgcat tggtcttcca taccgtcaac
    24001 gatgaatttc aacgcgtcgc cgatgttgtc ggcttcgatt tcgtattggt tgaattcttc
    24061 gtccataaag acgtacatcg ggtcggcaaa atagctgtac gtacagtttt tgcgggacag
    24121 gatgaccacg tcgaatttgt cgtcggcttt gtaaatggtt tcggaagcag cgccggtcag
    24181 caggtttttc agtttcatgc tgactttggc ggaagagcgg ccgcctttga tgtattcggt
    24241 tttttgaacg accataggat cgttgccgac cataaataca ttgccggcgc gcagttcttg
    24301 tgctgttttc attgtgattt tccaatcaaa tcggttgtcg taaaacgcgc tattctagcg
    24361 tattttttga tgctttgaaa caaaggcggc gagtttttcg gatgcggaag gctgcccgaa
    24421 aagataacgg ctccaatcct ccgcgccttg ccgccagccg ttttgatgtt gttgcagggt
    24481 ttgccaacat tcgaggcgtt gtgttgcgga taaagcctct ccgccgttga ggtcgtccga
    24541 aaggaggcgg tgcaccgatg cggtttcggg cgtgtagaag ccgtatgcct tatcccaaaa
    24601 ggcgtggagt ttgtcgagat ggacattctc gtcttgcggg tagatgtgcc aaaaaaaggg
    24661 ttttccggca agctgggtac gcacgaaact gtcttcgccg cgtatcacgg cgcagtcggc
    24721 gaggtgcagc aatttatcga agtcctgttg cggcacgaac gggattttga caaggcggac
    24781 ggatgccgtc tgaaagacac cgccgtcatt ttgcagggcg ttttgcggaa taacgccgct
    24841 ttgtttgagg ctgtcgataa tttgcgcccc cgccagcagt agggtcatca ggctgcctgc
    24901 ctgttgccac atgtccagcc actttgccca aacatcgccc cgatagccga aaagcagcca
    24961 ttcgggggcg tttttttcgg gcagcaccag ccgccggcgc agggcttcgg tatcgaaacg
    25021 gacggcttcg cggtaatcgc gttcgcgtat caacccgccg cttttttcgc tgaaacccat
    25081 aaaccaaaaa tatttttgaa cgccctcctg cggcgaaggc atcaggtgca gcctttcatt
    25141 gctttcctcc gcgctcaaat attcccaatt cagccagagc ggtttgtgtc ggcggatgat
    25201 gttcagcaca ttttcgggca ggtcgcaggc aaaagtttcg ataacggcat cgggaacggg
    25261 cgcggtatca atgtctgccg catcggaatg ccaagtgcgg acatgaatat cctgatgaac
    25321 gaagggaaca tcgggcaaat cgggacaaag cgcgcgcaag gcggacacgt cgtccgtcca
    25381 caaatgcacc tgccaaccga gttcgcggtg caaaacacgg gcgagccgcc acgaaacgcc
    25441 gatgtcgccg aaattgtcga tgaccttgca aaaaatccaa cagacaggaa aagcgtatgt
    25501 attcataacc atcgggaaaa caagctaaaa cgcgccgaaa tagtggaaca aatacacaac
    25561 cagcaaaaca atcggaataa acagaaacat ggtttgacgt ttcaaatcaa acccttgttt
    25621 aagatgatgc ttgatggttt caaagaggac gaatttaaac aaaaacgtcg cttcgcaggc
    25681 aaggatgatg ccgttgatca gatgctgggg tttgtcttcg ggcggattgg cggcgaaaac
    25741 ggcaagcgtc gccaagggga tgaggcagac ggcgagccag cggacaagca ggtttcggga
    25801 cattgcaagg ctttcaaaaa agcaaaacgc cattatcgcc tcaagaacgc accttgtcta
    25861 acggacgcgc cggagatgcc gtctgaagcc ttgccgccgg aaaacgccgc accgccgaac
    25921 ctttaaatat acccgggacg ggcgttcccc gagaaatcgg acgctgccgc cgatgcctgc
    25981 ggacaaggcg gcatcgggcg cgggcatatt gaaagcccga cgcatcggtt tgaacgcatt
    26041 ttataaaggc gtacggcaaa cccgcccctt gccgtcggcg tgcttccgca aagcaaaata
    26101 taagggcggc gcgctttccg ctataatccg catttttcca aacctgaaac attatgccga
    26161 ccccgaaagc ctttaccctg ctcggcccga ccgcctgcgg caaaaccgcg ctcgccctca
    26221 aaatcgccga aacccttccg gtcgaaatca tcagcctcga ttctgcactg ctctataccg
    26281 gtatggacat cggcacggcg aaaccgtccg cctccgaacg cgcctttgtc ccgcaccacc
    26341 ttatcgacat cattacgccc gttcagacat acagtgccgc ccgttttgtc gaagactgca
    26401 cgcgtctgac cggcgaaatc acggcgcgtg ggaaatgtcc gctgattgtc ggcggcacaa
    26461 tgatgtattt ccgcgccctg acccaaggtt tgaacgattt gcccgaagcc gatgcctgcc
    26521 tgcgtgccga cttggacgaa caaaaacaga tgtacggttt ggattttctc taccgcaccc
    26581 tgcaaaaagt cgaccctgaa acagcatgcc gtctgaaacc gaacgacagc cagcgcatcg
    26641 gacgcgccct tgaagtctat tacctgaccg gcaggccgat gagcgcgcac ctgaacggac
    26701 agcccgaaca caccctgccc ttcgaactct acaccgccgc cctgattccc gaaaaccgcg
    26761 cccgactgca cgaaaacatc gccctgcgtt tccacctgat gcttgaacag ggctttatcg
    26821 gcgaagtaga aaacctgcgc cgccgctatc ccggcctgac cgccgactcc cccgccatcc
    26881 gctgcgtcgg ctaccgtcag gcgtgggaac acctcgacgg tgcaaccgac agacaaacct
    26941 tcatcgaaaa aggcattgcc gccacgcgcc aacttgccaa acgccaactg acttggttac
    27001 gcaaaacacc tttagactgc gttgccgacc cgttttcaga cggcacttca ggcacgcgcc
    27061 tgattgaggc ggcaaaacgg tttttcggcg aataaaccat gctggccacg ccgccgctcg
    27121 cccccaaaac cgtcgccgcc ctgcacaggc tcggtatccg tacgctcgaa gaactgcggc
    27181 aaaacggttc ggtcaaagcc tttctgctgc tcaaagcatc gggtttgacc ctcacgaaaa
    27241 gcacgctgtg gcaactcgag tcgctgctga acggcacgcc gccgcaagaa atgtcccaag
    27301 cgcataaaga ctgcctgctt gccgagttga aaaaccatcc gcccgtcgcc gcctttccgc
    27361 cgcaggaaga aatgggatac ttcatgcgcg aagcactgcg ccaagcagaa caatccgccg
    27421 ccgacggcga aatacccgtc ggggcagtca tcgtttcaga cggcaaaatc atcacatcgg
    27481 cacacaacac ctgcattgcc gactgcaatg tcagccgaca cgccgaaatc aacgccttgg
    27541 cacaggcagg cagcgaaatg caaaactacc gccttgacgg atgcgacatc tacatcaccc
    27601 tcgaaccctg cgccatgtgc gcgtccgcac tgatacaggc acgaatcagg cgcgtgatct
    27661 acggtgcggc agaacccaaa accggcgcgg caggcagcat cgtcaacctg tttgccgaca
    27721 aacgcctcaa tacgcacacc gccatacggg gcggaatcct gcaagaagaa tgccgcgccg
    27781 tattaagccg ttttttccaa aacaaaagaa aaggttgaac ccatgaccgc cctactcgtc
    27841 atcctcgccc tcgccctgat agccgtcggc acggcaggca tcgtctatcc cgccctgccc
    27901 ggcttggcat tgatgtttgc cggaacatgg ctgcttgcct atgccggcgg ctatcaaatc
    27961 tacggcgcag gcatcttgtg gacggtcgga ctcatcagcc ttggcggcat actggcggac
    28021 tatatggcag gcatgttggg ggtaaaatac actggggcag gcaaactcgc cgtccgaggt
    28081 gcattggccg gcagcatcat cggcatattt ttctccctgc ccgggctgat actcggcccc
    28141 tttatcggcg cggcggcagg cgaactgatc gatcggcgca atatgcttca ggcaggtaaa
    28201 gcgggcttgg gtacgctgtt ggggcttgtc gtcggcacgg cgttcaaaat cggctgcgcc
    28261 gtatccatct tgtttatcct gttggtgaaa tacatcgcat acctgtttta accccttcag
    28321 acggcatacg gataaattgc tataatgccg tctgaaacct tttgacaaac accattatgg
    28381 caaaccaaag acccatctac ggcttccacg ccgtcaacgc ccgattgtgg caaaacccca
    28441 aatcgattac cgaactctat atccaagaag gcaaatccga cgcacgcacg cgcgaagtgc
    28501 tggaaaaggc ggcaaacgaa aacatccgcg tatattttgc cgatgccgac cgcctcaacg
    28561 ccatcagcaa aggcgcgcgc catcagggcg tggtcggatt tatcgatgcc tccaaaaacc
    28621 acgtccacct cgaagacgta ttggaaaacc tcagcgaacc gccgctgttg ctgatactcg
    28681 acggcatcac cgatccgcac aacctcggcg cgtgcctgcg taccgccgac gcaatgggcg
    28741 tacacgccgt catcgcaccg aaagacaaaa gcgcggggct gaacgccacc gtcagcaaag
    28801 tcgcctgcgg cgcggcggaa accgtcccct acatcaccgt aaccaacctc gcccgcaccc
    28861 tgcgcgaatt gaaagaatac ggcatttgga tcatcggcac cgacatgggc ggcgacgccg
    28921 acctttacca ttgcaatctg cccgacagca cggcgtgggt gatgggcaac gaaggcgacg
    28981 gtatgcgccg cctgacacgc gaacattgcg acatgctggt gtccataccc atgttcggca
    29041 cggtcgaaag catgaacgtc tccgtgtccg caggaatggt attaagcgaa acccgccggc
    29101 aacgcgtatt gaaaaacaaa aaagcgtaag cctcaaaaac aatgccgtct gaaacaggga
    29161 ttctgtttca gacggcattc attttcaaaa tcagtgttcc aaatggtcgg tatcaaactt
    29221 gaccgccgcc tcggttttat cttcgggcaa gaccaaattc agcaagactg ccgtaatgcc
    29281 gccgccggaa atagagtttt ggaacaagac gggcaggttt ttaaacactt ccggctcaaa
    29341 ccccacgccc aagcccaaac cgaccgacgt tgccgcaatc accgcctcgc gcctgcggat
    29401 gccgtggccg accaaaatcc gcacgcccgc aatcgcaatc aggccgaaca tcaaaaccat
    29461 cgcgccgccc aacaccggac tcggaatcgt cgtaaacgcg cgtccgacaa cggggaacag
    29521 acccaacagc accaaaatca cggcaatata tttgcccacg tggcgcgaag ccactccggt
    29581 catctgaatc acgccgttgt tttgcgcaaa cgtcgtcagc ggcagcgaac ccaaagccgt
    29641 cgcaatcacc gataccaagc cgtcagccaa cacgccgccg cgcaggcgtt tggtgtattc
    29701 ttcgccttca atcggctggt cggacaccat tgccgttgcc gtcaaatcgc cgaccgcctc
    29761 aaacacgccc aacaagaaaa tcgcacccgc cgcaataaat gcgtgccagt caaaagcaaa
    29821 accatattta aacggtacgg gcagcgtaac cggcggcagg ttttgcagtg cggaaaaatc
    29881 caccttgccc aaaaacagcg cgacaatgta gccggcaatc agaccgaccg caatgccgct
    29941 catccgcagc agcggatttt tcatgcagtt gaacaccagc acaatcagca gcaccagcga
    30001 agccagcccc aagttttcca tcgagccgaa cgtgccgtcc gccttcgcgc cgaagccgcc
    30061 gccgaaatcg gcaataccga cgtgtaccaa actcagacca atcagcatca caaccacgcc
    30121 gctgaccgtc ggcgtaatca cttttttcaa atacggcaaa agccacgccg agaaacacac
    30181 caaaaacgca ccggcaaacg atacgcccaa gagcgtcgaa accatcgccc cctcactcaa
    30241 accgccctct ttcatccccg cgccgagcgc aatcatgacg gtaacgctgg atggacagca
    30301 tccccgaacc gaccggcccg aagcggttga cctgcaaatt aagtgccgat gccggaagcc
    30361 accatcgcca tcgacacgag atacgccgtc atttccaccg gtagttccag cgcgccgccc
    30421 acaatcagcg cgggcgtaat catcggcacg aaaatcgcca aaagatgggt aaccgcgctc
    30481 aagagcgcat taccgaacgg cggcctgtct tccaaaccgt acaccaaatc aggcgaatcc
    30541 gcctgttttt tcattgtttc agccattgga gtctttctat ccgttgccaa acgttaaaaa
    30601 attttaagaa cgaaattgta acgaatcaaa gattccttat caattcaaat cctcatgtgc
    30661 aaaaacacca ccggttgtcc gaattcaaat ttctttgaat ttccgcaccg gacagtcctt
    30721 catttccaga gagccttcat gcgcttaaca cattttaatc ctcaacggcg catccactgc
    30781 ttaaacccaa taatttcatg attataaaaa aaatattatt tattaagata tgataggact
    30841 taagccttgg aaccaaccgt gcggccgata tgtttcacac aaacaacaaa gtaagctaca
    30901 atccgaagca taatcgacct cactcaatga ataaaaggtt tccgtatgtt acaaggtagc
    30961 ttggttgccc tgattacccc gatgaatcaa gacggcagca tccattacga ccaactccgc
    31021 caattaatcg actggcacat cgaaaacggc acggacggca tcgtcgctgc cggcacgaca
    31081 ggcgaatccg ccaccctctc cgtcgaagaa catttaagcg taatcgaaga aacggtcaaa
    31141 cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg cgaacaacac cgtcgaagcc
    31201 atcgcccttt cccaagccgc cgaaaaagcc ggcgcggact acaccctctc cgtcgtcccc
    31261 tattacaaca aaccttcgca agaaggcatg taccgccatt tcaaagccgt tgccgaagcc
    31321 gccgcggttc cgatgatact ctacaacgtg cccggcagaa ccgtcgtcag catgaacaac
    31381 gaaaccatcc tgcgccttgc cgaaatcccc aacatcgtcg gcgttaaaga agcaagcggc
    31441 aacgtcggca gcaacatcga actcatcaac cgcgcgcccg aaggcttcgt cgtcctttcc
    31501 ggcgacgacc acaccgccct gccgttcatg ctctgcggcg gacacggcgt tattaccgta
    31561 gccgccaacg ccgccccgaa actctttgcc gatatgtgcc gtgccgcgct gcaaggcgac
    31621 attgcccttg cacgcgaact caacgaccgg ctgattccga tttacgacac catgttctgc
    31681 gaacccagtc ccgccgcacc gaaatgggcg gtatccgccc tgggcagatg cggaccgcac
    31741 gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg caaaagtccg caccgccctg
    31801 aaagcctcag gacaacttta aatatagcca tgccgtctga accgttcgga cggcataaca
    31861 tgacaaacac tttaatatcc atcacacagg atgacacgat gacccatatc aaacccgtca
    31921 ttgccgcgct cgcactcatc gggcttgccg cctgctccgg cagcaaaacc gaacagccca
    31981 agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact cgaagtcccg cctgatttga
    32041 acaaccccga ccaaggcaac ctctaccgcc tgcctgccgg ttcgggagcc gtccgcgcca
    32101 gcgatttgga aaaacgccgc acacccgccg tccaacagcc agccgatgcc gaagtattga
    32161 aaagcgtcaa aggcgtccgc ctcgagcgcg acggcagcca acgctggctt gtcgttgacg
    32221 gcaaatcccc cgccgaaatc tggccgcttc tgaaagcctt ttggcaggaa aacggcttcg
    32281 acatcgaatc cgaagaaccc gccatcggac aaatggaaac cgagtgggcg gaaaaccgtg
    32341 ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac agtcggtttg ggcggcatct
    32401 actccaccgg cgagcgcgac aaattcatcg tccgtatcga acagggcaaa aacggcgttt
    32461 ccgacatctt cttcgcccac aaagcgatga aagaagtgta tggcgacaaa gacaaagaca
    32521 cgaccatgtg gcagccttcc gcttccgacc ccaaccttga ggccgctttc ctgacgcgct
    32581 ttatgcaata tttgggcgtt gacggacggc aggcggaaaa cgcattggca aaaaaaccga
    32641 cccttcccgc cgccaacgaa atggcgcgta tcgaaggcaa aagcctgatt gtctttggcg
    32701 actacggcag aaactggcgg cgcaccggcc ttgccctcga ccgcatcgga ctgaccgtcg
    32761 tcggtcaaaa caccgaacgc cacgccttcc tggttcaaaa agccccgaac gaaagcaatg
    32821 cagttaccga acaaaaaccg gggctgttca aacgcctact gggcaaaggc aaagcggaga
    32881 aacctgccga acagccggaa ctgattgtct atgccgagcc tgtcgccgac ggttcgcgca
    32941 tcgtcctgct caacaaagac ggcagcgcat atgccggcaa agacgcatcc gcactgttag
    33001 gcaaactcca ttccgaactg cgttaaaccg ttcaaaacaa aagcagcttc caaacagaag
    33061 ctgctttttt gtgcggaact ttttacaaca aacggggcaa aatatcctca accgcctgaa
    33121 ccaacgcatc cgccaaaggc ggatcgaacg cacaatgccc cgcctggacc accctcaatt
    33181 ccgcttcggg aaaggctttc gacagctccc acgcactctg cataggcgta cacaaatcat
    33241 accgcccctg cacgataacg gtcgggatat gccgtatttt gccgatattg ttcaaaatcg
    33301 ccttatcacc ctgcaaccaa ccgccgttga caaaataatg gttttccaaa cgcgcgattg
    33361 ccagtgaggc ataagcatct tcatccactc cctccggctc gaaacgaatc agatagcttt
    33421 cccaatccgc ccaagccttc gcggcagaca ggcacacttc ttcatcttga tgaaacagca
    33481 acccgtgata cgcctcaatc agccggttcc gccgattttc agcaatcggc gcgacaaatt
    33541 tttgccattg ttccggataa atccggctca cgccgcccgc ctcgttcagc cacgccgttt
    33601 cagacggcct gcacaaaaat atcccgcgca acaccaatcc ctttacccgt tcgggatggg
    33661 tttgggcata agccagcgac aaagtgctgc cccacgaacc gccgaacacc agccatttcc
    33721 cgatacccag catttcacgg actttttcaa tatccgccac caaatcccaa gtcgtattgt
    33781 cttccgcaca agcatacggg tgcgaacgtc cgcaaccgcg ctggtcgatg atgacgatgc
    33841 ggaacacatc aggattgaaa aaaccccgac attcaggcga agcccccgcg cccggcccgc
    33901 cgtgtaaaaa aatgaccggc acaccgtcgg gattacccga ctcttcccaa taaatttgat
    33961 gaatttcaga cacctgcaag tatccgctat gaaaaggctg ttttatttca tacatcatct
    34021 tctcccatat tgcttctgac aatccgaaca tcccgacacg cgacatcccg cacgaaacac
    34081 aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgataaa aaccatcaat cggaaaatat
    34141 tgcataaaca ggcgaaaata tatcattgaa aagaaaaccc atacaatgtc tgacagaaac
    34201 aagccgcttt gccattgttt cctaacagtt aaaccccgcc cttcaaggcg gcggggcagg
    34261 gcttgattca aaatggcgca agcccctgcc ctcaaatcca acacgcagga ttaaaccata
    34321 atagcggctt tcttattatt tcttattgaa acaccgcccg gaacccgata taatccgccc
    34381 ttcaaca
//
LOCUS       DAXFHF010000017        33816 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXFHF010000017 DAXFHF010000000
VERSION     DAXFHF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33816
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.017"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(2..466)
                     /locus_tag="ACFIRM_000723"
     CDS             complement(2..466)
                     /locus_tag="ACFIRM_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028395.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGAGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="ACFIRM_000724"
     CDS             complement(543..3380)
                     /locus_tag="ACFIRM_000724"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC3028396.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMTTRLTEARQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="ACFIRM_000725"
     CDS             complement(3485..4744)
                     /locus_tag="ACFIRM_000725"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC3028397.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVENETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="ACFIRM_000726"
     CDS             complement(4823..6214)
                     /locus_tag="ACFIRM_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3028398.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            6414..6560
                     /locus_tag="ACFIRM_000727"
     CDS             6414..6560
                     /locus_tag="ACFIRM_000727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028399.1"
                     /translation="MGMWRAAEIINAADCHTSEIYSGLNLNRYGVASPCRTSCTVCGF
                     VALS"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFIRM_000728"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFIRM_000728"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC3028400.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="ACFIRM_000729"
     CDS             complement(7496..8176)
                     /locus_tag="ACFIRM_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC3028401.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFIRM_000730"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFIRM_000730"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC3028402.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFIRM_000731"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFIRM_000731"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC3028403.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSEHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="ACFIRM_000732"
     CDS             complement(9944..10444)
                     /locus_tag="ACFIRM_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC3028404.1"
                     /translation="MIRLTRAFAATLIGLCCTTGAHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFIRM_000733"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFIRM_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC3028405.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKVELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSASVRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIRKYGKTDGADGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFIRM_000734"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFIRM_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC3028406.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFIRM_000735"
     CDS             complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFIRM_000735"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC3028407.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="ACFIRM_000736"
     CDS             complement(15560..16357)
                     /locus_tag="ACFIRM_000736"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC3028408.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="ACFIRM_000737"
     CDS             complement(16360..17106)
                     /locus_tag="ACFIRM_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC3028409.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFIRM_000738"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFIRM_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC3028410.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="ACFIRM_000739"
     CDS             complement(17861..18853)
                     /locus_tag="ACFIRM_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3028411.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19262)
                     /locus_tag="ACFIRM_000740"
     CDS             complement(18858..19262)
                     /locus_tag="ACFIRM_000740"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC3028412.1"
                     /translation="MVYGIDYCADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNR
                     IDPLNITYLRVRGMWGIENPWFVFNYIYQRNMEKSFNFMTIINEDKWNSFNNTDKLLA
                     IQDSKLAISDIKIKNPNNPARLRNAKLITYHL"
     gene            complement(19291..19695)
                     /locus_tag="ACFIRM_000741"
     CDS             complement(19291..19695)
                     /locus_tag="ACFIRM_000741"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC3028413.1"
                     /translation="MNIIDAIINLANNPVVGVESHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19804..20931)
                     /locus_tag="ACFIRM_000742"
     CDS             complement(19804..20931)
                     /locus_tag="ACFIRM_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC3028414.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(21001..21076)
                     /locus_tag="ACFIRM_000743"
     tRNA            complement(21001..21076)
                     /locus_tag="ACFIRM_000743"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21041..21043),aa:Thr,seq:cgt)
     gene            21183..21662
                     /locus_tag="ACFIRM_000744"
     CDS             21183..21662
                     /locus_tag="ACFIRM_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028415.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            21757..22380
                     /gene="rsmG"
                     /locus_tag="ACFIRM_000745"
     CDS             21757..22380
                     /gene="rsmG"
                     /locus_tag="ACFIRM_000745"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC3028416.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            22480..23253
                     /locus_tag="ACFIRM_000746"
     CDS             22480..23253
                     /locus_tag="ACFIRM_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3028417.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANHALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(23999..24583)
                     /gene="rnhB"
                     /locus_tag="ACFIRM_000747"
     CDS             complement(23999..24583)
                     /gene="rnhB"
                     /locus_tag="ACFIRM_000747"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC3028418.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(24651..26546)
                     /gene="mnmG"
                     /locus_tag="ACFIRM_000748"
     CDS             complement(24651..26546)
                     /gene="mnmG"
                     /locus_tag="ACFIRM_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC3028419.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(26719..28146)
                     /locus_tag="ACFIRM_000749"
     CDS             complement(26719..28146)
                     /locus_tag="ACFIRM_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3028420.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            28305..29297
                     /gene="pdxA"
                     /locus_tag="ACFIRM_000750"
     CDS             28305..29297
                     /gene="pdxA"
                     /locus_tag="ACFIRM_000750"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC3028421.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(29527..32286)
                     /locus_tag="ACFIRM_000751"
     CDS             complement(29527..32286)
                     /locus_tag="ACFIRM_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC3028422.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            32779..33771
                     /locus_tag="ACFIRM_000752"
     CDS             32779..33771
                     /locus_tag="ACFIRM_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC3028423.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPVRAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT         6704 a         8624 c        10075 g         8413 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtccat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct gcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtt tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc ttcgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtcg tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctgacggct ggcgcagccg
     1201 gtttggagct gtactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcaccgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgacaccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggtcacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccactccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggctgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcgtt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtgaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaacacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagtg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccaaaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gcgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccagac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcata gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttctgaa atctatagtg
     6481 gtttaaattt aaaccggtac ggcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattgaaaa atgcggttgt tttcgccgat ggtggtgtgg ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgatttggac gttggggccg ataacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcggc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtga tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg tgcccattct ttatgctcgg acatcgggaa gatgccggca
     9001 aggtgtttgc cgctttcggt aatgctgtgg gtgacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgta ccgacgccgc cgccgatgat gcagtagctg
     9121 cctatggtta cgctgccgga gatgccggtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacggtggtg
     9241 tcgctcatcg cgccgcggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggtcagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac acggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgcgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacgga
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcgga cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg caccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataacgcg ctgtcggtct tgttgcgccc
    11161 ccagccgacg gtgcctttgt acagccagcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtatttc ctgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcacgga
    11521 tgcggatttg cccgtgccga agaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtacc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggagcgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcgcacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat tttccggccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggtttcgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcgaccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcctggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaacctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 cgacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atccgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggtcatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaattat agataatgtc
    19321 tttctcttcc catttttccg catctttgca ggcttttgta agcatgctgt catcaacaga
    19381 gagctttgat tttgggtggc tgctattcag cgccagtgcg gaatctttac tttcaatttt
    19441 cttaacttcg atggcatccc cgtttcgcaa catggcatca ggagggttgg agttgttgcc
    19501 caaatatgag aatacttttg cgtgtcgggc tatgcgttgg gtttcattta ggttgaaact
    19561 gccggaaaac aagtctttta catattcttc caaagcatca cctgcctgat tggcacgatt
    19621 gttgctttga gaatgggatt cgaccccgac aacagggttg ttagccaaat tgatgatggc
    19681 atcgataatg ttcatggttt ttccttgggg ttttgcggca gattatagca aaaccgtcag
    19741 ggtgtttatc ggaccgatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccgg
    19801 ccgtcagccg tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc
    19861 atccagccat ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg
    19921 catattggtg ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc
    19981 ttccagttcg gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat
    20041 gagggcggcg gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt cgagcagccg
    20101 gatttcgctg ccgttgagtt tgggagattg cagcttggcg gcggtggtca ggagcagctc
    20161 ggtggtgttg acgattttgc ggtgggcgtg ctgcatggct tccatcatgg aggggctgat
    20221 gcggctttcg cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat
    20281 ttggcgcatt ttgaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct
    20341 gatttcggca atcattttgc tgcagtcggc caggttgtcg gcaagcatga aacgccacat
    20401 cagtgtggat ttcagcggca gcagtttggc ggcggcaatg gcgatggcgg cgccgatgag
    20461 gacgttcatc gcgcgcatca ggccgctgtc gagccattcg ctgccgttgt cgccgatgag
    20521 catgcacatc gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca
    20581 gccggccagt gcgcttgccg tgccgatggt caggtagaag aggaggttgc cgtggaaata
    20641 atgctggttc agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg taccgagcat
    20701 acgttccacc gccttggagt aaatcgcgcc ttggaactgg agcatgccga ggacgacgaa
    20761 gacggtcatc cctatccatt cgccgtgttg gaggtggagt agccgggcga gtgcggtggc
    20821 gaacaggacg gctccgccga gccgcacggc atgtatgagg cggcggtggc ggtagcgttc
    20881 gtaggagttg agccagcggc cggaaaggcg tttgcgttgc gaggagttca tatcggatgc
    20941 cgtctgaagc ggaaatgtga aaaagcacag gcttcccgag ggagggtctg tgcttggtat
    21001 tggtgccgga gaagggaatc gaacccccga ccttcgcgtt acgaatgcgc tgctctaccg
    21061 actgagctac accggcgttt ttcgttatga tatatataaa cggttgtttg tgcaactttt
    21121 cgggcgggcg gcaaggcggt gcgcggtata atgcggcctg cttcggaaga ggggggacgg
    21181 cgatgtttgt gaacgagaaa tatccttatg cgaccctgtt tgcggggctg gtgtttttga
    21241 cgctgccgtt tgcgttggcg gtgcatgatg cctttgcatg tgcgttcgga cgggcggggc
    21301 tgctggtgtc ggtgtcggcc ggcggattcg gccggcgtgg cggttgggac ggcactgttt
    21361 ggtttgtgtt cggtgtgttt gcgtttttga atgtggttgt gtcggcgggc ctgacgaaac
    21421 tggcgtacaa aaagatgatg cggcggcatt cgcgttacgc gctgtttctg tcgggcgtgg
    21481 cggcttgcgc ggcggtggct tggattttca agctgttgtt gggcagtgcc gccttgggcg
    21541 ggctgagcgg ggaggcggtg tcggaatatg cgtttgccgt gtggctggtg tctatgctga
    21601 cgctgccgaa acgcctgacg cgcgcgccgg tgcagcctgt ggtgttccac aggaaaaaat
    21661 aggacggaac ggaaatgccg tctgaaaccc gacacgcggt ttcagacggc atgtttttcc
    21721 gctaacatta cgcctgaata tggacaggaa gcagatatgg aacgtaaaga acgcctgcgt
    21781 gcaggcattg ccgcgatggg gccggatatt tcggaaacgg cgcaggacag gcttttggcc
    21841 tatgtggatt tgttgaaaaa gtggaacaaa acctacaatc tgaccgccct gcgcgacgag
    21901 gaaaaaatga ttgtccatca tcttttggac agcctgacgc tgctgcccta tatcgaaggc
    21961 gcacagacga tgctggatgt cggttcgggc ggcggccagc ccggcattcc ggcggcggtg
    22021 tgccgtccgg atgtgcagat aaccttgttg gatgcgaata cgaagaaaac ggcttttttg
    22081 cggcaggcgg ctatcgagtt ggggctggac aatgtgcgcg tggtgtcggg gcgcgtggag
    22141 gcggtttcgg acgtgcgtgc cgacgtggtt accagccgtg cgtttgcgga actggcggat
    22201 tttgtgtcgt ggacggcgca cctgttgaaa gacggcggct actgggcggc gatgaagggc
    22261 gtatatccgc agggggaaat cggccgcctg ccgcaggatg tgtgcgtcga aaaagtccaa
    22321 aggctcgacg tgccgggctt ggatgcggaa cgccatatcg tcatcctgag caagcgttga
    22381 gcgcgcttca gacggcatga ataccttttt tgtgcggata aaggtaaaat tccgcactgt
    22441 ttttcttttt ttcaacatca gacgggacac gggcgggaca tgagtgcgaa catccttgcc
    22501 atcgccaatc agaagggcgg tgtgggcaaa acgacgacga cggtaaattt ggcggcttcg
    22561 ctggcatcgc gcggcaaacg cgtgctggtg gtcgatttgg atccgcaggg caatgcgacg
    22621 acgggcagcg gcatcgacaa ggcgggtttg cagtccggcg tttatcaggt cttattgggc
    22681 gatgcggacg tgcagtcggc ggcggtacgc agcaaagagg gcggatacgc tgtgctgggt
    22741 gcgaaccacg cgctggccgg cgcggaaatc gagctggtgc aggaaatcgc ccgggaagtg
    22801 cgtttgaaaa acgcgctcaa ggcagtggcg gaagattacg actttatcct gatcgactgc
    22861 ccgccttcgc tgacgctgtt gacgcttaac ggcttggtgg cggcgggggg cgtgattgtg
    22921 ccgatgttgt gcgaatatta cgcgctggaa gggatttccg atttgattgc gaccgtgcgc
    22981 aaaatccgtc aggcggtcaa tcccgatttg gacatcacgg gcatcgtgcg cacgatgtac
    23041 gacagccgca gcaggctggt tgccgaagtc agcgaacagt tgcgcagcca tttcggggat
    23101 ttgctttttg aaaccgccat cccgcgcaat atccgccttg cagaagcgcc gagccacggt
    23161 atgccggtga tggcttacga cgcgcaggca aagggtgcca aggcgtatct tgccttggcg
    23221 gacgaactgg cggcgagggt gtcggggaaa taggtcaatc caaatcgggc tgcccgtgcc
    23281 tttatgctgt ttggccgggt gcgttaatat ggcggattaa aataaaaata cttaatatcg
    23341 tcatttatag cggattaaca aaaaccggta cggcgttgcc ccgccttagc tcaaagagaa
    23401 cggttcccta aggtgcccaa gcaccaggcg aaccggttcc gtactatctg tactgtctgc
    23461 ggcccgccgc cttgtcctga tttttgttaa tccgctatat cgtcattccc gcaaaaacaa
    23521 aaaaatcaaa aacacaaaac tgaaatatcg tcattcccgc gcaggcggga atctaggtct
    23581 gtcggtacgg aaacttatcg ggaaaaacgg tttttccaac cctgagactc cggattcctg
    23641 ttttcgcggg aatccggttt tttgagtttc agtcattttt gataaattct tgcagctttg
    23701 agtttctaga ttcccgcttt tgcgggaatg gcggtttgga agttacctga aattcaaaaa
    23761 aaaaacggaa accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt
    23821 ttttgatttt ctgtttttcg cgggaatgac ggttgggaag tttcccgaaa cttaaaaaca
    23881 actgaaaccc aaaaaactaa attaccgcct gcgggggaat gacggttcgg gttctttctc
    23941 tttgaagttg cgatgctgga aatgccgtct gaagttcaga cggcattttt gtgccggttt
    24001 aaaacaaggt ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg cggtgttcgg
    24061 gcagcacgcc gtattgtttg agggcttcca gatgctgctt tgtgccgtaa cctttgtgtt
    24121 tgtcgaaacc gtattgggga cggcgttgcg ccagtgcgta catttccgca tcgcgtgcgg
    24181 tctttgccaa aacggatgcg gcggagattt cgatgatttt gctgtcgcct ttgacgactg
    24241 cttcggcagg gatgcctaaa tgttcgggaa tgcggttgcc gtcgatgaat attttttcgg
    24301 gacgcgcagc caaaccgtaa acggcacgtt tcatggcgag catggtggcg tgcaggatgt
    24361 tgaggcttgc gatttcttcg ggcgtggagg cggcgacgtg ccacgcgacc gcctgttctt
    24421 taatcatttc ggcaagcgcg tcgcgttttt tctcgctgag ttttttggag tcggtcagtc
    24481 cgggcaggtc gaatgtttcc ggaaggatga cggcggcggc aaacacgctg ccgaccaaag
    24541 gtccgcgtcc tgcttcgtcc acgccggcgg tcagtatgtg catgatgttt cctgtcggga
    24601 tggtacgatg ccgtctgaaa atggtttcag acggcatcgt accgatgtgt ttatttcgcg
    24661 tctttaaacc cgcgcttcaa atgcaccatc agcagtgcga cggcggcagg cgtaacgccg
    24721 gaaatgcggc ttgcctgccc gacggtttcg ggtttgtgct ggttgagctt ttgttgcact
    24781 tcggccgaca agcctttgac tctgccgtaa tcgatgccgt cgggcagctt taaggtttcg
    24841 atgtcgcggc ggctgtcgat ttcttcgttt tggcggtcga tatagccttg gtatttgact
    24901 tggatttcga cttgctcgat gacttcggca gagaggtttt cagacggcat cgcgccttcg
    24961 agcgtcatca gcgcggcgta atcgaggttc gggcggcgta acaaatcgtg caggttggct
    25021 tcgcggctga gtttttgtcc gaacacgcgg atttgttcgt cttcggcgag tttttgcggc
    25081 gtgtaccacg ttgttttcaa acgttggatt tcgcgttcga cggcttcgcg tttttcgttg
    25141 aacatgcgcc attgcgcttc acccaccaag ccgattttgt agccgtcttc ggtcaggcgc
    25201 atatcggcgt tgtcttccct gagttgcagg cggtattcgg cgcggctggt aaacatccgg
    25261 taaggttcgt tcaggccttt ggtgatgagg tcgtccacca atacgccgag gtaggcttgt
    25321 tcgcggcgca gcaggagcgg atcttgtccg cggacgtatt gcacggcgtt cgcgcccgcc
    25381 agcaatcctt gcgcggcggc ttcttcatag cctgtcgtgc cgttgatttg cccggcgaaa
    25441 aacagtccct caatggtttt ggtttcgagg cttgctttga ggttgcgcgg atcgaagtag
    25501 tcgtattcga tggcgtagcc ggggcgcagg atatgggcgt tttcaagacc cttcatactg
    25561 cgcacgagcg cgatttggat gtcgaacggc aggctggtgg agataccgtt ggggtagtat
    25621 tcgtgcgtgg tcagaccttc gggttcgagg aaaatctggt ggctgtcttt gtcggcgaag
    25681 cggttgattt tgtcttcgat agacggacaa taacgcggac ccacgccttc gattttgccg
    25741 gtaaacatcg ggctgcggtc gaagcctgag cggatgatgt cgtgggtttg cgtgttggta
    25801 tgcgtaatcc agcaggacac ttggcgcggg tgcatttcgg cgttgccgcg cacggacatg
    25861 acgggaacgg gcgtgtcgcc gggctgttcg gtaagttggg aaaagtcaat cgtgcgtccg
    25921 tcaatacgcg gcggcgtgcc ggttttcaga cggccttgcg gcagcttcaa ttcgcgcaaa
    25981 cgtccgccga gcgatttggc ggctgggtcg ccggcgcggc cgccttcgta gttttccaaa
    26041 ccgatgtgga ttttgccgga caagaacgtg cctgcggtca gcaccacggc gcgtgcttta
    26101 aactccgcgc ccatcgcggt gatcacgccg ctgatgcgtt cgccctcgag tgttacgtct
    26161 tcgacggctt gttggaaaag gtcgaggttt tcttggtttt ccaacatttc gcggatggag
    26221 gctttataca ggatgcggtc tgcctgcgcg cgcgtggctc gtactgccgc gcctttgctg
    26281 gcgttcaggc ggcggaattg gatacctgat ttgtcggttg ccaatgccat tgcgccgccg
    26341 agcgcgtcga gttcgcgtac caaatgccct ttgccgatgc cgccgataga ggggttgcac
    26401 gacatttgtc cgagcgtttc gatattgtgt gtgagcaaaa gcgtctgcgc gcccatacgg
    26461 gcggcggcaa gcgcggcttc cgtgccggcg tgtccgccgc cgacgacgat gacatcgtag
    26521 gttttggggt aaatcatgtg ggtcatgtgt gtattgccta tcggtttttc agacggcatt
    26581 ccaagcagga tgccgtctga aaaacgaaac ggattcaaaa gtaaagggtt gggattgtac
    26641 gcctgttcgc cctgttttta cagtgcgcgg aaagggaaaa gccgcttcgc ggggaagcgg
    26701 ctccggtaag ggtgggattt accaaacatc ggatttgatg cggcgtttca ggcccggatg
    26761 ttcggaaagt ttgaactcgg ggtctttgcc cattttcagc ttggcggtgt aatcgcgcag
    26821 cagcataaac gccaatggcg agagcagcag gatggcgacg aggttgatcc acgccatgat
    26881 gcccatcgcc atatccgcca tatcccagac caaaggcaca ttggcaaccg cgccgaaata
    26941 gacccacgcc aaaaccagca tacggaaaac ggcggtaatc agccaatggc ttttgatgaa
    27001 ttggacgttg gactcggcat aggcatagtt gccgataacg gtggaaaagg caaacataaa
    27061 caggatgacg gcgaggaaac ccgcgcccca ttgccccact tggctgacaa tcgccgcctg
    27121 cgtcagcgcc gcaccgctca aatcgccata aggctgttgg taaatcaaga tgatgaaggc
    27181 ggtgcaagaa caaacgatga tggtatcgac aaacacgccc agcatttgaa tcataccttg
    27241 cgaaacaggg tgtttcactt cggcggcggc ggcggcgttc ggcgcggaac ccatacccgc
    27301 ctcgttggaa tacaggccgc gtttgatgcc catcatcatc gtttgcgaaa tcagaccgcc
    27361 gagtaagccg cctgctgccg cgtcgaattt gaacgcgccc gaaaaaatct gaccgaacac
    27421 gtccggaatc atcggaatat tggtcaaaat gatgaaaagc gcgataaaga ggtacaaaac
    27481 cgccatcagg ggcacgacga tttccgccgc tttggatatg cgcctgatgc cgccgaagat
    27541 aatcggcgcg gttaaaatca ccagggcgac gccgacataa tgaggctccc agccccacgc
    27601 cgctttgacg gtatcggcga tggtattggt ctgaaccgct tcaaacacaa aaccgaaaca
    27661 gaaaatcagg ctcagggcga acaacacgcc cagccatttc tgccccagac cgtgcgtgat
    27721 gtagtaggca gggccgcccc ggaaatggtg gttgtcgcag tcgcggactt taaagagctg
    27781 cgccagcgaa gattcgacaa acgccgaact catgccgatt aaggcggtta cccacatcca
    27841 aaacaccgcg cccggcccgc cgactttgat ggcgattgcc acgcccgcga tattgcccac
    27901 gcccacgcgg ctggcaaggc cggttacaaa tgcctgaaac ggcgtgatgc cgtgagggtc
    27961 gtccccctgt ttgcggccgc cgagcatttc tttgatgctg cgcccgaaca ggcggaattg
    28021 gacaaagccc gtggttacgg tgaagaaaag ccccgtaccc aaaagcatat aaaccaagta
    28081 tgaccacatc ggatcgttga tggcgccgac ccagccgtgc agccattcgg taaagttctc
    28141 gttcatatcg cttccttaaa gttgaaaccc gcacatatgg cggtatgcaa gcagggttta
    28201 aattttgtaa acgcccattc tagcagattg tcaacaaaat cagaaaaatt tacatcgccg
    28261 cgcgcctgtg gcgttagaat cgcgttttgt ttggagcaaa cacgatgaaa cagcctgttt
    28321 ttgccgttac ttccggcgag cctgccggca tcggccccga tatttgtttg gacttggcgt
    28381 ttgcacgcct gccctgccgc tgcgcggtat tgggcgacaa acacctgttg cgcgcgcgcg
    28441 ccgaagcctt gggcaaaagc gtcgtcctgc gcgacttcga tccggaatca ggcggcgcgc
    28501 acggagaagg cgggctggaa gtgctgcaca tccctgccgc cgaagcggtt gaggcagggt
    28561 ggctcaatcc cgccaacgcc gcctatgtgc tgcaactttt ggacaccgcg ctcgcaggca
    28621 tttcagacgg cattttcgac ggcatcgtta ccgcgccgct gcacaaaggc atcatcaacg
    28681 ccgcgcgcgc aagcacaggt tttttcagcg gacacaccga atatctggcg gaaaaaagcg
    28741 gcacggggca ggtcgtgatg atgcttgccg gcaaaggcct gcgcgtcgcc ctcgtaacga
    28801 cccacctgcc gctgaaagac gttgccgccg ccatcacgca accgctgatt gaaagcgtcg
    28861 cacgcatttt gcatcacgac ttaaaacaca aattcggcat caaaaatccc aaaatcctag
    28921 tcgccgggct caatccccac gccggcgaag gcggacacct cggacacgaa gaaaccgaca
    28981 ccattatccc cgcgctcgaa aacctgcgcc gcgaagggat aaacctcgcc ggcccgtatc
    29041 cggcggacac attgttccaa ccgtttatgc tcgaaggtgc ggatgccgta ttggcgatgt
    29101 accacgacca agggctgccc gtgttgaaat accacagctt cgggcagggc gtgaacatca
    29161 cgctggggct gccctttatc cgcacctccg tcgatcacgg caccgcgctc gatttggcgg
    29221 caaccggcag ggcggactcc ggcagcctga taactgccgt ggagaccgcc gtcgagatgg
    29281 cgcgcggcag cctttaaaga tgataaaaga cccgtcattc ccgcgcaggc gggaatccgg
    29341 accttcgggc ggcggcaata ttcaaaggtt atccgaaagt ttgaggttct agattcccgt
    29401 tttcacggga atgacgaaag gtggcgggaa tcacgggaat ggcgaagttt caggcggtat
    29461 tgcaggtatc cgaaaccatg caaaaagagg ttctgcggaa cagaacctct ttttgccgcc
    29521 gtcggttcag ccttgccggg tttcgacttg gatcatttct tcggcaggga cggttgcggc
    29581 ttcagacggt tttggctgtt cggaacggcg caaaccgcgt ccggcttgga cttcgggttg
    29641 tgccgcccat gccttcaatg cggcagggtc ggtttcgatc aggacgagtc cgccggtttg
    29701 tgcggtttcc cgtgcctgtt ccgccgcagc cgtaaaggtt gcggtttcag acggcatttc
    29761 ctgtgctgcg gctttcggtg ccgcgccttc gggcaggatg tcggcggcgg cacggcggat
    29821 tttttccgcc gcatcataaa tcagtgcgtc gccgtttgaa acggcggaag atgccgtctg
    29881 aacgggtgtg gcgatgagcg gatcggcaat gctgacggta atcggcgcgt ttgcgtcggt
    29941 ttcgccgaaa acgtgcgcgg cggcggaacg gactttgtcg gcggtgtcgt gaatattcag
    30001 gtactgctcg attttggcgg cagacgggat attgcgtttt ttgccgtttt gacggcggtc
    30061 gcgctgattg ttgcgttcgc ggcgttcttt ggcatcgcgg ctgtcgcgtt cgcggcggtt
    30121 gcgttcggat ttgggcttgc tgcctttgtc ttctgcggta tacggttcgg gcggcgtgtt
    30181 ttccgctgtc tgaacggctg tttcggcaac ggcggcggtt tccggtgcgg tttggccacg
    30241 ttcgttgcgg ctgccgttgc ggcggcgttt tccggtttga ccttcggttt cgggcggtgc
    30301 ggcatctgca acgggtgcgg caggctgtac gttgcggctt tggatttccg cttcgttggc
    30361 gcgttcggtg gcacggtcgc cgcgttcgtt gcggcggcgg ttgccgttgt tgcgcgtttc
    30421 ggctttgtcg gcgcgcgctt tctgtccggc agttttgccc gccgcttcgc ggacttctat
    30481 tttgctgcct tcgcgtttgc tgcggcgcgg gttttggcgg cggttgttgg cgcggctgcc
    30541 gctgcggttt gccgtgctgc gtttttcgga ggtttcggca gcgggtacgg cttgggtttc
    30601 gctgccgccg aaaatgcgtt tgagccatgc tttgaagctg tcccaccagg aggttttttt
    30661 ctcgggggcg gcagtcgggg cggggctggt gtggcgcacg cctttgacgg cgggttcggg
    30721 gcgggcggct ttggcttttt cgccgccgaa aggtttggcg gattcgtctt cttcaggctc
    30781 ggcaacgcgc ttgtagctcg gttcgccgtc ttcttctacg tcgtcggtgc ggatgcggtt
    30841 gatttcgtag tgcggatttt cgaggtggat gttcggaatc aggacgacgt tcacatccaa
    30901 acgctcttcc attgcaaaca gctcggcgcg tttttcgttc agcaggaagg tggcgacatc
    30961 gacgggcact tgggcgcgca cttcgccggt gttgtccttc atcgcttctt cctgaaccat
    31021 gcgcaaaacg tgcagggcgg tggattcgat gccgcggatc acgccggtgc cggcgcagcg
    31081 cggacaggcg gcgtggctgc tttcgcccaa agcgggtttc aggcgttggc ggctcaattc
    31141 caaaagtccg aaacgggaga gtttgcccat ctgtacgcgg gcgcggtctt ttttgagtgc
    31201 gtcgcgcagg acgttttcca catcgcgctg gtgtttgggg ttttccatgt cgatgaagtc
    31261 gatgacgacc aagccgccca agtcgcgcag gcgcatttgt cgggcgactt cttcggcagc
    31321 ttccatattg gttttgaagg cggtgtcttc gatgtccgcg ccgcgtgtgg cgcgtgcgga
    31381 gttcacgtcg atggagacga gggcttcggt gtggtcgatg acgatcgcgc cgccggaggg
    31441 caggctgacg ctgcgcgaga acgcgctttc gatctggtgt tcgatttgga agcgggaaaa
    31501 cagcggcgtg tggtcttcgt agagtttcag acgacctgcg ttgcccggca tgacgtagct
    31561 catgaactcg gcaacttggt cgtaaacttc ttgattgtcc accaaaattt cgccgatgtc
    31621 gggacggaag tagtcgcgga tggcgcggat gagcaacgag ctttccataa agagcaggta
    31681 ggggtcgtga tgcgcttttc ctgcttcttc gattgcctgc cagagttgtt tgaggtagtt
    31741 caagtcccat tccaactctt ccgcgctgcg gccgatgccg gcggtacgcg cgatgatgct
    31801 catgccgttc ggaatgtcga gttgcgccat cgcggctttg agttcttgac gctcttcgcc
    31861 ttcgatacgg cgggatacgc cgccgccgcg cggattgttc ggcatcaata ccagatagcg
    31921 tccggcgagg ctgatgaagg tggtcagcgc cgcgcctttg ttgccgcgct cgtctttttc
    31981 gacttggacg atgacttcca tgccttcttt gagcacgtcc tgaatgcgcg cgcgcccgcc
    32041 ttcgtagccg aggaaatacg aacgcgatac ttctttaaac ggcaaaaagc cgtggcggtc
    32101 ggttccgtaa tcgacgaaac acgcttccag cgacggctcg atgcgggtaa tgatgccttt
    32161 gtagatattg cctttgcgct gttctttgcc cagcgtttcg atgtccaaat ccagcaggtt
    32221 ttgtccgtcg acgatggcaa cgcgcagctc ttcggcctgc gttgcgttga ataacattct
    32281 tttcatgatc acctcgtgga cgggcggcat tcgggcgcgc cggtccggtt cggcattccg
    32341 taaggctggg tttccgatgt cttcggataa aaccggtaat cagtttttga gttgaaaatc
    32401 cgcagggatg cacgttccgg ggaaccgtgt gcggaagggt cggataaaga aggctataaa
    32461 gatcgatgcg gcggtctgcc tgccgcgttc cgaacgctgc ggtcggaaaa atgggcgccg
    32521 gcttcttctt gttatcgtga tgcctgtgtt ttcgggcggt ttgcgttcgg ggcttggggc
    32581 tccgctgccg tcttacttcc gcgccgaaac ggcaaaatca attcaaactt gactacgttc
    32641 tgcgcctgcc ggctgggaac aggcgcaggg aaaatgcttt gcggagtgcg tttttaatat
    32701 aaaattccgt tttaaagtaa accgtttcag ggggcgcggc ggacgcgctt tctgctgaaa
    32761 cggatgttcg gattatagat gaaaacgcac gaaataagca aaggttcggt cagcctgata
    32821 ggggttgccg aacatgaggc gggtcaacgc cttgataact atctgataaa aatcctcaag
    32881 ggcgtgccca agggctatat ccaccgcatt atccgcgccg gcgaggtgcg gttgaacaag
    32941 aaacgctgca aacccgacag ccgtattgcg gagggagata cggtgcggat tccgcctgtg
    33001 cgcgtggcgg agaaggaaat gccgtctgaa aggcgtgccg ccgtaccggt gcgtgcgttt
    33061 gaggtggtgt atgaggacga cgcgctactg gtggtcaata agccttccgg tgtggcggta
    33121 cacggtggca gcggcgtgag tttcggcgtt atcgaacagt tgcgccgcgc ccgtccggag
    33181 gcgaagtatt tggagttggt ccaccgtttg gacaaggata cgagcggctt gttgatggtg
    33241 gcgaagaaac gcagcgcgct cgtcaaactg cacgaagcta tccgtaacga ccaccccaaa
    33301 aaaatctacc ttgcgctggg ggtgggcaaa ctgccggacg acaatttcca tgtcaaactg
    33361 cccctgttca aatataccgg cgcacaaggc gaaaagatgg tacgcgtcag cgaggacggg
    33421 cagtcggcgc atacggtgtt ccgtgtgtta agccgtttct cagacggcat tttgcacggt
    33481 gtcgggctgt cgcacctgac tttggtgcgg gcaacgctga aaacgggccg tacgcaccaa
    33541 atccgcgtgc atctgcaatc tcaaggctgt ccgattgcgg gcgacgaacg ctacggcgat
    33601 tatcaggcga accggcgttt gcagaaattg ggtttgaagc gcatgttttt gcacgcgtcc
    33661 gagctgcacc tgaaccatcc gctcacgggt gagccgctgg tgttgaaggc ggagccgccg
    33721 ccggatttgg cgcagtttgc ggtgatgttg gaaaacggga cgaaaatgtg aaccccgatg
    33781 ccgtctgaag gcttcagacg gcatcggggc gtgaaa
//
LOCUS       DAXFHF010000018        33713 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXFHF010000018 DAXFHF010000000
VERSION     DAXFHF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33713
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.018"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACFIRM_000753"
     CDS             complement(116..835)
                     /gene="pyrH"
                     /locus_tag="ACFIRM_000753"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC3028424.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACFIRM_000754"
     CDS             complement(1052..1906)
                     /gene="tsf"
                     /locus_tag="ACFIRM_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC3028425.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFIRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACFIRM_000755"
     CDS             complement(2035..2763)
                     /gene="rpsB"
                     /locus_tag="ACFIRM_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC3028426.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2936..3169)
                     /locus_tag="ACFIRM_000756"
     CDS             complement(2936..3169)
                     /locus_tag="ACFIRM_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028427.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3247..3828
                     /locus_tag="ACFIRM_000757"
     CDS             3247..3828
                     /locus_tag="ACFIRM_000757"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC3028428.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3889..4479
                     /locus_tag="ACFIRM_000758"
     CDS             3889..4479
                     /locus_tag="ACFIRM_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC3028429.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPHLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLVCCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4863..6329
                     /locus_tag="ACFIRM_000759"
     CDS             4863..6329
                     /locus_tag="ACFIRM_000759"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3028430.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6516..6887)
                     /locus_tag="ACFIRM_000760"
     CDS             complement(6516..6887)
                     /locus_tag="ACFIRM_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3028431.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(7125..7424)
                     /locus_tag="ACFIRM_000761"
     CDS             complement(7125..7424)
                     /locus_tag="ACFIRM_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3028432.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            complement(7523..8302)
                     /gene="map"
                     /locus_tag="ACFIRM_000762"
     CDS             complement(7523..8302)
                     /gene="map"
                     /locus_tag="ACFIRM_000762"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC3028433.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8342..8992)
                     /locus_tag="ACFIRM_000763"
     CDS             complement(8342..8992)
                     /locus_tag="ACFIRM_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028434.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNVPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(9000..9608)
                     /locus_tag="ACFIRM_000764"
     CDS             complement(9000..9608)
                     /locus_tag="ACFIRM_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC3028435.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9672..10265)
                     /locus_tag="ACFIRM_000765"
     CDS             complement(9672..10265)
                     /locus_tag="ACFIRM_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC3028436.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            complement(10270..10617)
                     /locus_tag="ACFIRM_000766"
     CDS             complement(10270..10617)
                     /locus_tag="ACFIRM_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC3028437.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10664..11539
                     /gene="rsmI"
                     /locus_tag="ACFIRM_000767"
     CDS             10664..11539
                     /gene="rsmI"
                     /locus_tag="ACFIRM_000767"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC3028438.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11574..12104)
                     /locus_tag="ACFIRM_000768"
     CDS             complement(11574..12104)
                     /locus_tag="ACFIRM_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC3028439.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12383..13537
                     /gene="obgE"
                     /locus_tag="ACFIRM_000769"
     CDS             12383..13537
                     /gene="obgE"
                     /locus_tag="ACFIRM_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC3028440.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13591..14538
                     /locus_tag="ACFIRM_000770"
     CDS             13591..14538
                     /locus_tag="ACFIRM_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3028441.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIILETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14535..15587
                     /locus_tag="ACFIRM_000771"
     CDS             14535..15587
                     /locus_tag="ACFIRM_000771"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC3028442.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15603..17024
                     /gene="cysS"
                     /locus_tag="ACFIRM_000772"
     CDS             15603..17024
                     /gene="cysS"
                     /locus_tag="ACFIRM_000772"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC3028443.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGRSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17195..17974
                     /locus_tag="ACFIRM_000773"
     CDS             17195..17974
                     /locus_tag="ACFIRM_000773"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3028444.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17961..18305
                     /locus_tag="ACFIRM_000774"
     CDS             17961..18305
                     /locus_tag="ACFIRM_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028445.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18315..18845
                     /locus_tag="ACFIRM_000775"
     CDS             18315..18845
                     /locus_tag="ACFIRM_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3028446.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18938..20053)
                     /gene="asd"
                     /locus_tag="ACFIRM_000776"
     CDS             complement(18938..20053)
                     /gene="asd"
                     /locus_tag="ACFIRM_000776"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC3028447.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20228..20809
                     /locus_tag="ACFIRM_000777"
     CDS             20228..20809
                     /locus_tag="ACFIRM_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3028448.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20871..21725)
                     /gene="folD"
                     /locus_tag="ACFIRM_000778"
     CDS             complement(20871..21725)
                     /gene="folD"
                     /locus_tag="ACFIRM_000778"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC3028449.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            21948..22025
                     /locus_tag="ACFIRM_000779"
     tRNA            21948..22025
                     /locus_tag="ACFIRM_000779"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21983..21985,aa:Pro,seq:tgg)
     gene            22050..22126
                     /locus_tag="ACFIRM_000780"
     tRNA            22050..22126
                     /locus_tag="ACFIRM_000780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22084..22086,aa:Arg,seq:tct)
     gene            22154..22229
                     /locus_tag="ACFIRM_000781"
     tRNA            22154..22229
                     /locus_tag="ACFIRM_000781"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22187..22189,aa:His,seq:gtg)
     gene            22441..22947
                     /gene="rfaE2"
                     /locus_tag="ACFIRM_000782"
     CDS             22441..22947
                     /gene="rfaE2"
                     /locus_tag="ACFIRM_000782"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC3028450.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGATETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            22944..24722
                     /locus_tag="ACFIRM_000783"
     CDS             22944..24722
                     /locus_tag="ACFIRM_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC3028451.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRSVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            24835..25659
                     /locus_tag="ACFIRM_000784"
     CDS             24835..25659
                     /locus_tag="ACFIRM_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC3028452.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPVPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(26165..26377)
                     /locus_tag="ACFIRM_000785"
     CDS             complement(26165..26377)
                     /locus_tag="ACFIRM_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC3028453.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGSFVV"
     gene            complement(26392..27180)
                     /locus_tag="ACFIRM_000786"
     CDS             complement(26392..27180)
                     /locus_tag="ACFIRM_000786"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC3028454.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(27259..27453)
                     /gene="thiS"
                     /locus_tag="ACFIRM_000787"
     CDS             complement(27259..27453)
                     /gene="thiS"
                     /locus_tag="ACFIRM_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC3028455.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            complement(27875..28492)
                     /gene="thiE"
                     /locus_tag="ACFIRM_000788"
     CDS             complement(27875..28492)
                     /gene="thiE"
                     /locus_tag="ACFIRM_000788"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC3028456.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(28514..29614)
                     /locus_tag="ACFIRM_000789"
     CDS             complement(28514..29614)
                     /locus_tag="ACFIRM_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC3028457.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPTSVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(29611..30837)
                     /gene="cytX"
                     /locus_tag="ACFIRM_000790"
     CDS             complement(29611..30837)
                     /gene="cytX"
                     /locus_tag="ACFIRM_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC3028458.1"
                     /translation="MSGNASSPSSSAAIGLVWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVKLSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAEIPVAVGVTLIGTVLAVMLPVTEYKNFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(30903..31007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            31082..32566
                     /gene="tldD"
                     /locus_tag="ACFIRM_000791"
     CDS             31082..32566
                     /gene="tldD"
                     /locus_tag="ACFIRM_000791"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC3028459.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            32710..33369
                     /locus_tag="ACFIRM_000792"
     CDS             32710..33369
                     /locus_tag="ACFIRM_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3028460.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            33359..>33713
                     /locus_tag="ACFIRM_000793"
     CDS             33359..>33713
                     /locus_tag="ACFIRM_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3028461.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKH"
BASE COUNT         7327 a         8617 c         9824 g         7945 t
ORIGIN      
        1 tgaaaaacaa attgtatcaa aattgtaaat ttaatccact ataatctcca tataataaat
       61 ggctgtctga agcccatatg cgactatatc tgccgaccct atggtcaatc atcaatcagc
      121 agtggaccag tgtgccttcg tcttcgccgg taatgacgcg tttgagcgag ccttgtttgg
      181 cgatgccgaa gacgacgata ttgagcttgc gttcgcggca gagggcaaag gcggtcgcgt
      241 ccatgacttt gaggttttta ttcaaggctt cgtcaaaagt aatggtttcg tagcgtgtgg
      301 cggacgggtc ttttttcggg tctgcggtgt acacgccgtc gacgttggtg gctttgagca
      361 tcacgtcgca gttcatttcc gcgccgcgca atgcggcggc agtgtcggtc gtgaagaacg
      421 gattaccggt accggcggcg aaaatcacga ctttgccttc ttccaaatat tgaatggctt
      481 taggacgggc gtaagtttca gcgatttgct gcatagacag tgcggattgt acgcgcgctt
      541 tgatgcccaa ggtttcaaat gcgtctttga gtgccaaggc gttcatcacg gtcgccatca
      601 tgcccatgta gtcggcagtg gcgcggtcca tgctgcctgc ttgtgcagat acgccgcgga
      661 aaatgttacc gccgccgaca acgataccga cttgcacacc cattttcacg atttcggcaa
      721 tttcgccgac agtttgaaca atggtatcgt gattgatgcc gaacggatcg gaacccatca
      781 gggattcgcc ggagagtttc agtaatacgc gtttgtattt gatttgctgt gtcatgggat
      841 accttgcttt cttgaatgtc gtcggtcgga gttgggacgt ctgaatttca aatggtcgga
      901 ttacaatgat atggtgtgtc gttctattat tgttttgttt tcagacggcc tttatattcg
      961 gtcgtcttaa aataggtacc gtaaacggtt ttctcaaaaa aaagcaccct gattcgtttg
     1021 gaatccaggt gctttctttt tcataagtgc cttacacttt ggcggctgcg gctacttcgg
     1081 ctgcgtagtc gacaacggct ttctcgatac cgtcgcctac tttgtagcgg atgaagctaa
     1141 ccacttcagt gtcgttttct ttagcgaatt gggcaacagt ttggtcaggg ttcatcacga
     1201 atgcttggcc gttcagagtg atttcagcca agaatttgcg gatacggcct tcaaccattt
     1261 tagcggcgat gtcggcaggt ttgccggaag cgatggcttg ctcggtgtag atgtggcgtt
     1321 ctttttcaac ggtttcagca tctacttcgg cttcgcttac gcattgcggt ttggcggcaa
     1381 cgatgtgcat accgattttg cgtgctacat cttcagagcc tttgtactcg accaaaacgc
     1441 cttcggtcgc caatgcgccg tggatgtagg caaccagttg gttggcagtg tcaatcactt
     1501 ggaagcggcg gacagacatg ttctcgccca atttagcgat gatggctttg cgttctgatt
     1561 caaccagttc gctcagttct tcaacagaag ccggtttttt ctcggcagca gttttcgcaa
     1621 cgaagttggc aaattctacg aagccggcgt ctttagcaac gaagtcggtt tcgcagttta
     1681 cttcgaccaa tgcgccgaca ttaccgttga ttgcataagc caatacgcct tcggcagcag
     1741 tacggccagc cagtttgccc gctttcgcac cggatttaat acgcaggatt tcttcggctt
     1801 tgtcgaagtt gccttcagct tcaaccaagg cttttttgca ttccatcata cccaggccgg
     1861 tagcggcgcg caggtcggca accatttttg cagtaatttc tgccattttg aatctcctag
     1921 atttttggga aatacggaaa ggattttcca cattcgggaa tcgtgtgccg cgaacgggcg
     1981 ttcagacggc atatttgaga aaaggggcat aacgcccctc ttctgtgtac cgaattactc
     2041 ggcagcggtt tcttgggcag cggctacggt ttcttgcagc gcttggtttt tgccttccaa
     2101 aactgcgtcg gcgatgcctc ggcagtacag gcggatggct ttggcggagt cgtcgttgcc
     2161 ggggatgacg tatttcacgc cgtctgggct gttgttggta tcgactacgg cgataacggg
     2221 gatgcccagt ttttcagctt caaccagagt acctttttgg tagccggtat cgataacgaa
     2281 aatcgcgtcg ggcaggcctt tcatgttttt gataccgccc aaagagcgtt ccagtttttc
     2341 aacgtcgcgt tgcatttcca gaatttcttt tttgctgaaa ccgctttcgg cagcattttc
     2401 caaggctgcg gttttttctt ccaggcgttt gatggattgc ttaacggttt tgtagttggt
     2461 cagcataccg cccaaccagc ggtggtcgac gaaaggcata ccggcgcggg tcgcttcttc
     2521 gcggatgatg tcgcgtgcct ggcgtttggt acctacgaac aataccgtac ctttgttggc
     2581 aaccagacga cgtacggctt cttgcgcctc ttggaacatc ggcagggttt tttccaagtt
     2641 tacgatatgg attttgttgc gcgcgccgaa aatgtattga gccattttcg ggttccagaa
     2701 acgggtttgg tgaccgaagt gaacaccggc ttcaatcatc tgacgcatag taatttgaga
     2761 catgttattt ccttgaaagg gttaaagagc acacattcaa tcgcatagaa cttgataggc
     2821 ggcgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa atagtgtgtt
     2881 tccgaaatgg aaaactgacg gattatagcg gataagatgc cgtctgaaaa gggattcagg
     2941 gttttgtgcc gctgtctttt tgccgttgtg cgcgctgctg ttcggattct gcctgttcct
     3001 gctgccccat caggcggttg cggcggcgga cggttacggc gaagaccgtg aacacggtgg
     3061 gcagaaccac ccaaaaaacc aaataaatca aagcgcgcgc aatactcggc tgcgcggcgg
     3121 aatacataac ggcaacaaaa agatagccga tggcgacgat gtgccacatt gtgtgcgtcc
     3181 tgtttgaatg ttaacaaaat cgttataatc gcaacattct accgcaatcc tgaaaggccg
     3241 tctgaaatga ggaacgagga aaaacgcgcc ctgcgccgcg aattgcgcgg gcggcgttcg
     3301 caaatggggc gggacgtgcg ggcggcggcg gcggtaaaaa tcaaccgcct gctcaaacgt
     3361 tatatcaagc gcggtcggaa aatcggcgtg tattggccga tgggcaagga attgcgtttg
     3421 gacggctttg tccgcgcggc gcaaaaacgc ggcgcaaaac tctatctgcc ttatatcgaa
     3481 ccgcacacgc ggcggatgtg gtttacgccg tatcctgaac gcggaatgga acgggaacgc
     3541 aagcgcggta gggcgaagct gcatgtccct cagtttgcag ggcgcaaaat ccgcgtgcac
     3601 ggtttgtcgg tattgctcgt cccgcttgtc ggcatagacc gcgaaggcta ccgtttgggg
     3661 caggcaggcg gctattacga tgcgacgctt tcggcgatga aataccgttt gcaggcgaaa
     3721 accgtgggcg tgggctttgc ctgccagttg gtggacaggc tcccacgcga ggcgcacgac
     3781 ctgccgctgg acggttttgt atcggaagcg gggatattgt gtttttagcc gttcagacgg
     3841 catttcgggc ggaaatgccg tctgaaggcg gaatattggg agaaaaggat gtcggaaagg
     3901 ataatactgc cggttttgtc attgggcggc gtacggctcg aaccgttgga tgtgcatcat
     3961 gaaacgggtt tgcgcgaggc ggtttgcgat ggggaggttt ggaagctggg ggtaacgtcc
     4021 gcgccccatc cggatagggt ggcagactat atcgggacag cattggcaac gcgtttggcg
     4081 tttgcggttg tcgatgaaga ggcggacagg gtggtcggga caacggcgta ttatcatttt
     4141 gaaccgcaga ttccgcattt ggatatcgga ttcacatggt atgcggcatc ggcacggcgg
     4201 aaacggataa acacctgttg caaaatcatg ctgttggatt acgcatttga tgttttggtt
     4261 tgctgttgcg cgggatggcg cacggatatt ctgaaccttg cctcgcaacg tgccatcgaa
     4321 cggctgggcg cggaaaaaga cggcgtgctg cgtatgcata tgctccgtaa agacggcagt
     4381 gtgcgcgata cggttgtgta cagtatgctg agggaagatt ggtgcaaaaa cagggaaatc
     4441 ctgaccggca ggttggcagg gtatggcgtg caggtgtgag gcagtattct caaatttaaa
     4501 aaacttaacg cgatatgctg acaatctttt aaaaattaat atataaacaa ttttgatttt
     4561 ttgtatatta aaaatttgat atatatagat aaataactat ttttattttt tgtttcgcag
     4621 ttccccggct tggcgggcga cagaaaaact ttgcatttcc cagtaaaatc cactacattc
     4681 acgataacat attacaagcc cctgaagcgg tgtcggaagc catacttgca acgcattcca
     4741 accccgaaac agcgtgctcg gacacacacc attgccggtt tcccctgctt ttgcgattca
     4801 ggcgtttgta gtacggcaaa taaccataag ggcagaagat gcctgttttt taggagtaaa
     4861 ccatggctga agcgacagac gttgtcttgg tgggcggcgg cattatgagc gcgactttgg
     4921 gcgttttgct caaagaactc gaaccgtctt gggaaatcac cctgattgaa cgcttggaag
     4981 atgtggcgtt ggaatcgtca aacgcgtgga acaacgccgg cacggggcat tccgcgctgt
     5041 gcgaattgaa ctatgcgccg ctgggtgcgg acggcgtcat caatccggcg cgcgccctca
     5101 atattgccga acagtttcat gtcagccgcc agttttgggc gacgctggtc gcggaaggca
     5161 agttggaaga caattccttc atcaatgccg tgccgcatat gtctttggtg atgaacgaag
     5221 accactgccg ttacctgcaa aaacgctatg atgtgtttaa aacgcagaaa ctttttgaaa
     5281 atatggaatt ttccaccgat cggaacaaaa tttccgattg ggctccgctg attatgcgcg
     5341 gccgggacga aaaccaaccc gtcgccgcca actattccgc cgaaggcacg gatgtcgatt
     5401 tcggacggct gacgcgccag atggtgaaat atttgcaggg caagggcgta aaaaccgagt
     5461 tcaaccgcca cgtcgaagac atcaaacgcg aatccgacgg cgcgtgggtg ctcaaaaccg
     5521 ccgatacccg caacccagac tggcagctta ccctccgcac ccgcttcctc ttcctcggcg
     5581 cgggcggcgg cgcactgacc ctgctgcaaa aatccggcat ccccgaaggc aaaggctacg
     5641 gcggcttacc cgtgtccggc ctgttcttcc gcaacagcaa ccccgaaacc gccgaacaac
     5701 acaacgccaa agtgtacggg caggcttccg tcggcgcgcc gccgatgtcc gtcccgcacc
     5761 tcgacacacg caacgtagac ggcaaacgac accttatgtt cggtccttac gcaggtttcc
     5821 gttccaactt cctcaagcaa ggctcgttta tggatttgcc gctgtccatc catatggaca
     5881 acctctatcc tatgctgcgc gccggctggg cgaatatgcc gctgaccaaa tacctgctgg
     5941 gcgaattgcg taaaaccaaa gaagaacgct ttgcctccct gctggaatac taccccgagg
     6001 caaaccccga cgactgggaa ctcatcaccg cagggcaacg cgtccaaatc attaaaaaag
     6061 actccgaaaa aggcggcgtg ctgcagtttg gtacggagat tgtcgcccac gccgacggct
     6121 cgctcgccgc attgctggga gcgtcgccgg gcgcatcgac cgctgtgccg ctgatgatcc
     6181 ggctgatgca ccaatgcttc cccgagcgcg ccccgtcttg ggaaggtcgt ctgaaagagc
     6241 tggtaccggg ttacggcatc aagttgaacg aaaaccctga aagggcggat gaaattatcg
     6301 cctataccgc gaaagtgttg gatatttaaa ccgaatcttt caagaaaagc cgtaccccca
     6361 tatggtgttg acgccgtccc tgccggttat gcccttcatc cggcagggca ggttttgata
     6421 cacggggcag gtaatcgttt tttccctttt gcgaataatg gaacggtcaa aaaacagcgt
     6481 tttcagaaat gaaaacgctg ttttttgttg cctgattaac gtggggaaca gtctttgaag
     6541 acgatttggt tgtcaggtgc ggtaatcata ataggctgtt tgcggtagga tttgctgtcc
     6601 attgcgccgg tgctcagcac ataaccgcct tctttgccgt agaacgtgtc catattgtcg
     6661 gatttatcca aattgatggg catttgcaca cgtttgccgt tgatgacggc ggaggcgtat
     6721 gtggtcagac cctgtttgtt gaagccgtag gttactttga cttttttacc ttgctggcag
     6781 acgtagctga cggttttttt ggcaacggtg gggttgtccg tgccggcggc ggccatactg
     6841 ctgagcgcga ttgcggaaga caggattgca gtggtcagaa gtttcatttt tattcctttg
     6901 gatagatgtg gagtttgccc gtttcggact gtttgacagt ctaatgtcgg cgtatcctga
     6961 aataaacagg aaattggata aagtttgtaa aaaggcaggt tcgggcggtt cggcggtttg
     7021 aggcaggggc ggcaggcggg atacggcggc ctgtccggtg tttcagacgg cattgtttgc
     7081 atttttcgga aatgaaaaac ctccgggcat ccggaggttg cggtttaacc ttgttttttt
     7141 ctttcggcct tggtctgttt ggacaattcg gcatacaggt cgggatggtg ttttttcaac
     7201 agggcggcga tttccatatc ttttttgttg acttttgcca aatcgaggca ttcgatatgg
     7261 acgaggcgga gcagttcgcg tacgggtttg ggcatcttgc ggccggtttc gtagcgtgaa
     7321 cctccggatt gagtaacgcc gaccttgccc cagaagtcta tctggttcat cccggtcttt
     7381 ttacggatgg ctttaacatc ttctatattt tcaaatattt tcataaaact tcctttaagt
     7441 ttttctggtt gcggggcaaa ttccgatttg ctttgttttc gtaatgtgcc gggtcaagcc
     7501 tgtttttata ttgcatacag cttcagggtt tgccggtggc ggggctgacg gtgaggattt
     7561 cgtagccggt ttcggttacc aagacttcgt gttcccattg ggcggagagg gagcggtctt
     7621 tggtaaccac cgtccagccg tcgttgagga tacgcaggtg gcgtttgcct tggttgatca
     7681 tcggttcgac ggtaaaaatc atacccggtt ttagaacggg accctgccct ttttttccgt
     7741 agtgcaacac ttgcggggct tcgtggaaac cgcgcccgat gccgtgtccg cagaattctt
     7801 gtacaaccga ataacctgca ttttcggcaa cctgctggca ggcgtaacct acgccgccca
     7861 gcgtcgcgcc aggtttgacg gcttctatgc ccgccatcat agaggcgtgg gttacgtcga
     7921 tcaggcgttg ggcgatgggg gagactttgc cgacggtaaa catacggctg gagtcgccgt
     7981 ggaaaccgtc ttttttgatg gtgaggtcga tgttgataat gtcaccttct ttgagcggct
     8041 tgtcgtcggg aatgccgtgg cagatgacgt ggttgacgga ggtgcagcag gatttggggt
     8101 agggcgggtt gccgtagtgg aggggggcgg gatagccgcc ttggacgttg acgtggtagt
     8161 cgtaaacaag tttgtcgatt tcgtcggtgg tgatgccggg tttgacgaat tgtccgatgt
     8221 agtcgagggc ttcggcgacg agtttgccca gctcgcgcat tttttcgatt tcttcggggg
     8281 ttttgatgat gatgccgttc ataatgcttc cttggattgc gggtgtgcct gccgtgccgg
     8341 gtcaaaagag gttgtcgaga atctctttat ggggtttggc cggcgtgttt ttcggggtgt
     8401 ccggtttggt atggttttct ttgggcgttt ctttttcttt gggtgtttct ttgggctgcg
     8461 cctgtttttc gggtgtttct tttcggggtt cgggatgggg gacgtttgtg ggcagtacgg
     8521 gggtttcttt gagtgtgttt tcagcttgtg ttccggtgcc ggttaacgaa tcggctgccc
     8581 gggccgcatc cgaagggcgc ggtttgaccc ggtttttctt gggtgcggca gggggcggca
     8641 gtccgctgcc gctttcttct gtgccgtccg gctgtgcggg ttcggatgag gcggcgtggt
     8701 ggtgttcggg ttgggcggca tcttgtccga cggcgccgtt tggcagccag aattcggttt
     8761 cgcgggggct gtcggtgtgt tgcggttcgg tttggagggt tttgtcgccg ttcgggttga
     8821 atgtgctgac gatgcctact gccaatgcgc cgatggcggt gatacagatg agcaatacgg
     8881 cgcgtatcag gagtttgggg gtcagcctga agggtttgtt cggttttttt gccattttga
     8941 ttgtgctttt ggggtgtcgg gcaatgccgt ctgaaggcgg ttcagacggc attgccgagt
     9001 cagcgttgga cgtagttttg gtagagggta atgacttttt gtacgccgac ggtggtgctg
     9061 actttttggg taatctgcgc ctgttcttcg ggggtgagga tgcccataac gtaggttaca
     9121 ttgccgtagg taatgatttt gacgcgcgcc tgtgtagcgg ggctgatgcc cagcagcgtg
     9181 gcgcggactt tggacgtgtt ccaagtgtcg ccggcgatgt cgcccgcagt gcgcggcagg
     9241 gaggcgacgg taatgtagtt gtatacgcct tcggcggcct gttcggaacg tgcaatctga
     9301 ccgacgaact gtttttcgcc ttcggtggcg acttgtccga gcagcagcag gtggcggttg
     9361 tagccgacga cggagatttg gggcgtgtag cctttggttt ggttgttttg gcgcaggtag
     9421 gaacgggcgg tggtttcgat acgcaacgcc ataacgttgt catcggtttg cgcgccggtg
     9481 gttcggcggt cgatgacgga tttcgcgccg acggcggccc cgccgacgac tgcgctgaag
     9541 cagccgccga gggcaaggct gaggacggcg gcaatcaggg tgcggacggt gtgtggtttg
     9601 ggtttcatcg gggacttcct ttcttgggcg tttcagacgg cattgctttg cgccatgccg
     9661 tctgaaaagg gttacattcc ttccagcagt acggagtcga tacagtcgca tatggcgtgt
     9721 atcagcagga tgtggttttc ctgaatgcgg gcggtgcgcg gatgggggac gttgagcaga
     9781 acgtcggtgt ctttgagcat ggcggcgatt ttgccgccgt cgcggccggt cagggcgatg
     9841 acgtgcatat cgcgttcgtg cgcggctttg acggcttcga tgacgttggc ggaattgccg
     9901 gaggtggaaa tgccgacgag gacgtcgcct gcacgtccga gcgcgcgcac ttgtttgctg
     9961 aatacgtggt cgaaaccgta gtcgttgccg atggcggtca gcgcggaggt gtctgtggtc
    10021 agggcgacgg cggcgagttc catgcgttct ttctcaaaac gcccggtcat ttcggcggcg
    10081 aagtgttgcg cgtcggcagc cgaaccgccg ttgccgcagg ccaggatttt gccgtcgttc
    10141 atcaggcatt gcagcatcag ctcggcagcc tgtacggtcg gctcgaccag tattttttcg
    10201 gcttcctgtt tggcacggat gctttcggca aaatgtgcgg caacgcgttc ttgtaatgtc
    10261 gtcatatcgt caacctgtaa tattctttat ccactcgggc gggcggtttc cttcgataag
    10321 taccgcatcg aggcggcacg gtacgtttgt cagcctgttc tgttgcagat aatactctac
    10381 acttcgttgc agtttcaata atttggacgg agaaatgctg tatgccgcgc cgccgaaccg
    10441 ttggtttttg cggtatttta cttcaacaaa cagaatcatg ccgccgtttt tgacaatcag
    10501 gtcgatttcg ccgtaggcgc agtgccagtt gcgggcaagc agcgtgcagc cttgggattg
    10561 gaggaaggca agtgcggcat cttcccctgc cgtaccctgt ttgtggttta agcgcataat
    10621 cggtttaacc tttataatgt ctgtttttca gacggcattt tttatgtttc agaaacactt
    10681 gcagaaagcc cccgacagcg tcgtcggagg gacattatac gtggttgcca cgcccatcgg
    10741 caatttggca gacattaccc tgcgcgcttt ggcggtattg caaaaggcgg acatcatttg
    10801 tgccgaagac acgcgcgtta ctgcgcagct tttgagcgcg tacggcattc agggcaggtt
    10861 ggtcagtgtg cgcgaacaca acgagcggca gatggcggac aaggtaatcg gtttcctttc
    10921 agacggcctg gttgtggcgc aggtttccga tgcgggtacg ccggccgtgt gcgacccggg
    10981 cgcgaaactc gcccgccgcg tgcgcgaagc agggttcaaa gtcgttcccg tcgtgggcgc
    11041 aagcgcggta atggcggcgt tgagtgtggc cggtgtggcg gaatccgatt tttatttcaa
    11101 cggttttgta ccgccgaaat cgggcgaacg taggaaattg tttgccaaat gggtgcgggc
    11161 ggcatttcct gtcgtcatgt ttgaaacgcc gcaccgaatc ggggcaacgc ttgccgatat
    11221 ggcagaattg ttccccgaac gccgtctgat gctggcgcgc gaaatcacga aaacgtttga
    11281 aacgttctta agcggcacgg ttggggaaat tcagacggca ttggcggcgg acggcaacca
    11341 atcgcgcggc gagatggtgt tggtgcttta tccggcgcag gatgaaaaac acgaaggctt
    11401 gtccgagtct gcgcaaaatg cgatgaaaat ccttgcggcc gagctgccga ccaagcaggc
    11461 ggcggagctt gccgccaaga ttacaggtga gggcaaaaag gctttgtacg atttggcact
    11521 gtcgtggaaa aacaaatgat gccggatgga ccggcaccat agtgcgaacg gaatcaacgg
    11581 acacgccccg tcatgcggta cagcacgtct ttgccttgaa cacgatggac gacgaccatg
    11641 gcgacgtgtc cgacgacggc ggcaaacagc agccagccca aattgccgtg gaacgtgttg
    11701 cccaagtttg ccatccactc gattttttcc ggcgaaccct gcattacttc aacgccgaac
    11761 actttcaacg ggccgcgtcc gccaccgtat tggcggatca tgccgataac gggaacggca
    11821 agcatgagca gatacagaat gccgtgtcct gccgctgccg ccttgctgtc gctttgcgga
    11881 cgcttggcgc ggttggcaac cgcccacacg atgcgcaatg caatcaccgt cagcatgagg
    11941 aaaccgaaag atttgtgcag gccgaacagg ctgcccgccc attccgcctc ttcgtaaatt
    12001 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
    12061 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa atccttatca
    12121 tatgtaacgg tttgaacgga cattccgtcg ggtcggaatg tcaaaaaggc gcgattgtac
    12181 caaagaagtt ggacatattt tgtttgcagg ctgaagattt gcttaaaaat tcattaagat
    12241 gggcagaaca aatagtttct ccgcggtata ttgaaatacc gcttatacct tataatgtcg
    12301 gcagaaaatc tgttcagacg gcatcaaaac atatggcaat ccgcctgaaa ccctatttaa
    12361 aaccaacaaa caagagcatt taatgaaatt catcgacgaa gcaaaaatcg aagtcgccgc
    12421 aggcaaaggc ggtaatggcg caaccagttt ccgccgcgaa aaattcgtac cccgcggcgg
    12481 cccggacggt ggcgacggcg gcaaaggcgg cagcgtctgg gcagaagccg acgaaaacac
    12541 caacaccctc gtcgaatacc gcttcgtcaa acgctaccaa gccaaaaacg gcgaaaaagg
    12601 ccacggttcc gaccgctacg gcgcaggtgc ggacgacatt gtcctcaaaa tgcccgtcgg
    12661 cacccttatc cgcgacctcg acaccgacga aatcgttgcc gacctcacct accacggaca
    12721 gcgcgtctgc ctcgccaaag gcggcaaagg cggcttgggc aacatccact tcaaatcctc
    12781 cgtcaaccgc gccccaaaac aatccacgcc cggcgaagaa ggcgaaaccc gttccctgca
    12841 actcgaactc aaagtcctcg ccgatgtcgg cttgttaggc atgcccaacg ccggcaaatc
    12901 caccctgatt accgccgtct ccgccgcacg ccccaaaatc gccaactacc ccttcaccac
    12961 cctgcatccg aacttgggcg tggtacgcat cgacgaaaac cacagcttcg tgatggccga
    13021 catccccggt ctgattgaag gcgcggcaga aggcgcaggc ctcggccacc gcttcctcaa
    13081 acacctatca cgcaccggtc tgctgttaca cgttgtcgat ttggcaccct tcgacgaaac
    13141 cgtcaatccc gccgaagaag cactcgccat catcaacgaa ttacgcaaat acgacgaaga
    13201 actttacggc aaaccgcgct ggctggtgct gaacaaactc gatatgctcg acgaagaaga
    13261 agcccgggcg cgaacagccg ccttcctcga agccgtcggc tgggactacc ccgaacctga
    13321 tgaccgtttc caattcgaca tggaaacccc gcgcctcttc caaatcagcg cgctgacaca
    13381 tcaaggcacg caggagttgg tacaccaaat caaccaatac ctggccgaga aaaaacgtat
    13441 cgaggctgaa aaagccgaag cggagaaagc agcggcaaat gttgaaatta tcgaacagca
    13501 gcctaaaacg gatactggcg tgtttaagcc ggagtaaaag gacaaggccg tctgaaagtt
    13561 ttcaggcggc tcattctgca gagatgtgct atgtacaaaa caatagattt attttcaggc
    13621 attggcggaa tacgcctagg ctttgaaaaa tacgggtgca ccaatgtatt ctcatcggaa
    13681 tgggacaaat atgcccgaca agtgtatgaa gcaaactttg gagaaaaacc ttttggcgac
    13741 attaacggca tcgatccttc agatattccc gatcatgata ttctgcttgc gggatttccg
    13801 tgccaacctt tcagtatcgc aggtaaggga ttgggatttg aagatacgcg cggaacattg
    13861 tttttcaata ttgccgaaat cctgaaaacc aaacagccga aggcttttct tctggaaaac
    13921 gttaagcggc tgactacgca cgattcgggc aggactttcc gtattatttt agaaaccctt
    13981 aaacagcttg gttatacggt ttattttaag gttttaaata ctttggattt cggattgccg
    14041 caaaaacgcg agagaattta tattgtgggt ttttcagaca acatcccttt ttactttccc
    14101 gaacccataa accaatatcg gccattgggc gaactgctgg aaaacgatcg agatgtcgaa
    14161 ccaagctatt ttttatcaga taccttgaaa caaaaacgtt tggcagcctt aaaaaaagct
    14221 ccgcccaccc catcaatttg gcatgaaaat attggcggga atgtttccgc acttccatat
    14281 tcctgtgcct taagggccgg gggaagttac aattatttgg tggtaaacgg agtaagaagg
    14341 ctaacaggaa gggaaatgtt aagattacaa ggttttcccg atgattttga aatcaatatc
    14401 ccctactcgc aagtcaggaa agtcgccgga aattctgtat ccgtccctgt aatagaagcc
    14461 atcgcggaaa atatgctcgc ttctctttcc ggcaaggtcg aacaaaaagg gcaattggat
    14521 ttattggagg caggatgacg cttgaagaac aacaagccaa agaagcattg gacggcatta
    14581 tcaaaaaatc ccgtgtccac ctttataaac ccattcaaat tgcagaaatt ttatatcatg
    14641 accgttgtat aaaacagttg gattttttaa atttagatac ttaccgcaac caatctaaac
    14701 gctggaggga tgaaatctgc cgtcggtttt tagggcggat ttctacttca tctgcaaaat
    14761 tccaagataa tctgtttgaa aaaaatgcaa taccgcctga aaaactagcg gtattgggaa
    14821 cattaaacag acagtcggac ggcggggtag aatcgtacat ttataaacag tttttcaatc
    14881 gtttttccca aatgagcgaa gcattggctt atgtcggcaa tacagacaga tactccttcc
    14941 aactgtctga atttctaaat ttattttggc tcgagccggg attgaaaaga agtatagaca
    15001 agatatatga aatcgttgtt tatgcgttgt ttgatgcatt ggtttcagaa ttaggcataa
    15061 cggtttcaat tgattttcct aaggaaaacc tgtttttatg ggaggaatat caagattttg
    15121 ccgaaaaaat catcactatg ccgaaaaatg agcatttaaa acttcctgca aaaatccatc
    15181 gtgtaggcgt aaccaatgct gccgatagag ggttggatat gtggtctaac ttcggattgg
    15241 ccatacaggt caaacatctc tctttagacg aagagttggc agaagatatt gtatcctcca
    15301 ttagcgcaga tagaattgtc attgtctgca aaaaggcaga acaatcggtg attgtttcat
    15361 tactgacgca aataggctgg aaaagccgaa ttcaaaacat cgtaactgag gatgatttga
    15421 taagttggta cgaaaaagca ttgagaggcc aatatccgat tgcagaagcg ttgttggaaa
    15481 atattaaaac tgaaattatg cgtgaatttc ctgccgttaa tgaagccaat gaatttttag
    15541 atttcgccca aaatcgcgga tatgacatta ctgttactca tttctaaact tacttgatga
    15601 atatgaccgc catctacaac acccttaccc gtcaaaaaga acccttcgcc cccatcgacc
    15661 ctgaaaacgt gcgtatgtac gtctgcggca tgaccgttta cgactattgc cacttaggcc
    15721 atgcccgtgt gatggtggtg ttcgacatga ttgcccgctg gttgcgcgag tgcggttatc
    15781 cgctcactta tgtgcgcaac atcaccgaca tcgacgacaa aatcatcgcc cgtgcggctg
    15841 aaaacggcga aaccatcggc gaactgaacg cgcgtttcat tcaggcgatg cacgaagatg
    15901 ccgatgcttt gggcgtgttg cgtcccgaca tcgagccgaa ggcgacggaa aacatcccgc
    15961 aaatgattgc catgattgag accctgattc aaaacggcaa ggcatatcct gccgcaaacg
    16021 gcgacgttta ctacgccgtg cgcgagtttg ccgcttacgg acaattatcg ggcaaatcgt
    16081 tggacgacct gcgtgcgggc gaacgcgtgg aagtggacgg tttcaaacgc gatccgcttg
    16141 attttgtatt gtggaaagcg gcgaaagcag gcgaaccggc gtgggaaagc ccgtggggca
    16201 acggccgtcc gggttggcac atcgaatgtt ccgcgatgag tgaaaacctg ttcggcgata
    16261 cgttcgacat tcacggcggc ggcgcggatt tgcagttccc gcaccatgaa aatgaaatcg
    16321 cccaaagcgt cggtgccagc ggtcacacct gcggtcatga ccacgcgcaa acccaccacg
    16381 gtcgaagtat tgccagccac gtcaaatact ggctgcacaa cggcttcatc cgtgtggacg
    16441 gcgaaaaaat gtccaaatcg ctgggcaact tctttactat ccgcgaagta ttgaaacaat
    16501 acgaccccga agtcgtgcgt ttcttcatcc tgcgcgccca ctaccgcagc ccgttgaact
    16561 actccgacgc gcatttggac gacgcgaaag gcgcgttgac ccgtctgtac accaccttga
    16621 aaaacacacc ggcggctgag tttgatttgt ctgaaaacgc caacggctac acccgccgtt
    16681 tctacgccgc catgaacgac gatttcggta cggtcgaagc ggttgccgtg ttgtttgagc
    16741 tggcaggcga agtgaacaaa accaatgacg cacacctcgc cggctgcctg aaagctttgg
    16801 gcggcatcat cggcctgctg caacgcaacc cgattgagtt cctccaaggc ggcgcagttt
    16861 cagacggcct ctccaacgaa gaaatcgaag acttgattgc ccgacgcaaa caggctcgcg
    16921 cggataaaaa ctgggcggag tccgaccgca tccgcgacct tctgaacgaa cacaaaatca
    16981 ttttggaaga cagcgccggc ggcacgactt ggcggcgcgg ttgaactgta tcggaaaggg
    17041 caaaagtacc gccttttgcc cttttttcgt tttcagacgg catttcccgc ttgaagcccc
    17101 gccgttttca gcaggcagga ccgaatggga aataccgggc ggcgcaccgg gtgccgcttt
    17161 gtgtatggga acattgtgag aacttatttt tattatgctt aaaatcattt ccgccaacgt
    17221 caacggcatc cgctccgcct acaaaaaagg tttttacgaa tacatcgccg catcgggcgc
    17281 agacattgtc tgcgtgcagg aactcaaagc gcaggaggct gatttgtctg ccgatatgaa
    17341 aaatccgcac gggatgcacg gccattggca ttgcgccgag aagcgcggtt acagcggtgt
    17401 ggcggtgtac agcaaacgca aacccgacaa tgtgcaaatc ggcatgggca ttgaggaatt
    17461 cgaccgcgaa gggcggtttg tgcgttgcga tttcggcagg ttgggcgtca tttcgcttta
    17521 tttgcccagc ggcagcagcg cggaagaacg ccagcaggtg aaataccgtt tcctcgatgc
    17581 gttttaccct atgctcgaag cgatgaaaaa cgaagggcgc gacatcgtcg tctgcggcga
    17641 ctggaacatc gcccaccaaa acatcgacct gaaaaattgg aaaggcaatc agaaaaattc
    17701 gggcttcctg cccgaagagc gcgagtggat aggcaaagtc atccatacgc tgggctggac
    17761 ggacatgtgg cgcacgctct atcccgatgt gccgggttat acatggtgga gcaaccgcgg
    17821 gcaggcgtat gcgaaagatg tcgggtggcg catcgattat cagatggtta cgcccgaact
    17881 tgccgccaaa gccgtgtccg cacacgttta taaagatgaa aaattctcgg atcacgcccc
    17941 tttagttgtg gagtatgact atgctgccga ataaggtttt gggtaaatat gattggaatg
    18001 tggacggaaa aacaggaata ggcgcggcat gggttgtcgc tgcgtttatc ctgcccatat
    18061 tggtgtgggc ggtgtttatg ttgtcgcgga tgcaaggctg gcttgcgccg accaaggcaa
    18121 acccgatatg ggcgttggtg tggctgctga tttgtctgcc ctgcctgctg attgcggcca
    18181 aatgtttggg ttggaaaggc tggcggcggg tggtgaatat ttttgtctgc ctgaccgtct
    18241 gcgccatatt gagcgtaccc gcttcgctgc tcatcgcctt taccctccgg gacctgctca
    18301 aatagtcgcg gacaatggaa agtttcgagc ttgaggattt gacgctgtgg ctgatacgcg
    18361 atgcggatga agcggaaatg tggatagacc gttgggcggt cagttatccc gttgtgcaga
    18421 tgtccgaagc gtccgcaaac ggcagcacgg aagcgtggca aagcgcgctt cagacggcat
    18481 tcgaacggat acagggcagg catatcgccg ttgtcgcaca cggcgcgggc gcggccgcat
    18541 tcttggcgtg gctgtaccgc gccgacatcc tgacgcagaa gaaactcgcc ggtatcattc
    18601 tcgtatctcc gcgtcccgat atttttcccg acgatgcgga acacactttc caacgcgtcc
    18661 gctgtccttg ccgtgccgca ttggtggtgt ccgaacacgg cggcgtgccg cacggttggg
    18721 cgcaaaaaca ggcggatttg tggaacgccc gcctgttggt ttccccgcat tcgggcagtt
    18781 tgaacggtat gctcggcggc tggcagtggg gtatgaagct gatgcaggaa atgttgctgg
    18841 cgtgaacggt cgctttatcg gaatttcggt taaccggggt tgcaaggaaa ataccgtctg
    18901 aaacaggctt cagacggcat ttccattcaa acagggctta caggctgccc aacacgatac
    18961 gcaacacgcg gcgcatcggt tcggctgcac cccacaaaag ttggtcgccg acggtgaacg
    19021 cgctgatgta ttcgccgccc atgcccagtt tgcggatgcg tccgacaggg acggacagcg
    19081 tgccggtaac tttggcgggc gtcagctcgc ggatgctggc ttctttttcg ttggggacga
    19141 ctttcaccca gtcgttcgcg cctgccaaaa tcgcttcgat ttcggaaaca ggcaggtctt
    19201 ttttcaactt cagagtgatg gcttggctgt ggcagcgcat cgcgccgatg cggacgcaca
    19261 ggccgtcgat gacggtcgga ttgccgctgc gtccgaggat tttgttggtt tccacgccgc
    19321 ccttccattc ttctttggac tggccgttgc ccaaatccac gtcaatccac ggaatcaggc
    19381 tgccggcgag cggtacgccg aagttggctt tcggatagtc ttcgctgcgg aggaaatcgg
    19441 acactttgcg gtcgatatcg agaatcgcgc tggaaggatc ggcaagctcg tccgccactt
    19501 gggcgtgaat cgcgcccatg ccgctgatga gttcgcgcat atttttcgcg cccgcgccgg
    19561 aagcggcttg gtaggtcatg ctggtcgccc attcgaccaa atcgttttgg aacaggccgc
    19621 ccaaagccat cagcatcagg gagacggtac agttgccgcc gatgtagttt ttcacgccgt
    19681 ttttgagacc gttgtcgatg acgttgcggt tgacggggtc gagggcgata atcgcgtcgt
    19741 ctttcatgcg cagtgaggac gccgcgtcaa tccagtagcc gttccagccg ctgtcgcgca
    19801 gggcttggaa gacggatttg gtgtaatcgc cgccttggca ggtgacgata atgtccattt
    19861 tcgccagctc ggcaacgtcg ttcgcgtcca ataatgtttt agccgcctga ccgaaatcag
    19921 gtgccgcgcc gccgacgttg gaagaggtaa agaaaaacgc ttcgggaatg tgggcgaagt
    19981 cgttttcttc tttcatacgc tgcatcaaaa ccgaaccgac cataccgcgc cagccgacga
    20041 aacctacttt catgtattgc tccttgagga aaatcgttaa taaaattatg tatggcgtaa
    20101 agaaagcgtt ttaacgccgt attgggaaaa tgtaaagttc tttatcgaat tttctttttg
    20161 ggattggcgt ttgcagcagt agggcggtag aattccatct gtttaagaac aattatcagg
    20221 aacggatatg gaagaaaaaa acgactacac cgacgcggta tccgacaacc gcaacgggca
    20281 ggaaattgaa gtcggaattg ccggcgcagg cgaccggata cttgccgcgc tgctgaacca
    20341 attgtttacc tttttgattt tgttggtgcc gtttgtcgga ctgattgcct tcgccgtcaa
    20401 aaatgaaggc aggattggcg gccgtgagga aatattcggg ctgcttttgg gcatgacttc
    20461 cttttgggtc ggactggcgg gcattttggc gtacaccgtc atccaaatct actatatgag
    20521 cagggacggg cagtcattgg gtaagaaaat catgagaatc cgcgtgttga aaaccgacgg
    20581 ccgcaatccg ggttttgtcg gcacggtttt ggtacgcgaa atcgcgtggt cggttttggt
    20641 tgccattatt gccgccgtta tcggtcttgc agtaggtgaa aacggggaaa acgccatcaa
    20701 cctgctggca ttccttgcca actttgtcct gctctttatg gtcaaacgcg accgccgcac
    20761 gctttacgac atattggcgg atacggttgt cgtcaagctg cccaaataaa cggacgcggc
    20821 aaaatgccgt ctgaaaggct ttcagacggc atttttatct tcagaaccgc tcaagcatcg
    20881 tgcagcgaag ctgcgtgcaa agtgttttcc attaatgtgg caatcgtcat cggacccacg
    20941 ccgccgggaa cgggcgtaat catcgccgcc cgttcttttg ccgtttcaaa ttccacgtcg
    21001 ccgcacaggc tgccgtcgtc caaacggttg atgcccacat cgataacgac cgcgccgggt
    21061 ttgatccacc cgcctttgac aaagttcgga atgcccacgc cgaccaccaa aatatcggcg
    21121 gcggcaactt catcggcaag attttcggtc gcactgtggc agaccgttac cgttgcgcgc
    21181 gccagcagca attccaacgc ctgcgggcgg ccgacaatat tcgacgcgcc gaccacgacc
    21241 gcttttttcc ccttcggatc aatgccgtaa gcctccaaaa gcgtcatcac gcccttgggc
    21301 gtacacgggc gcatcagcgg cattttgacc gccagcctgc cgacattgta aggatggaag
    21361 ccgtccacgt ccttatccgg cgaaatacgt tccaagatcg cttggctgtc gaggtgcttc
    21421 ggcagtggaa gctgaaccag aataccgtcc acttcggaat cggcattcag acggtcgacc
    21481 aatgccagca gttcttcctg cgatgttgat tcgggcagtt cgtacgacag cgacttgatg
    21541 ccgcattttt ggcaggcggt tttcttgttg cggacataaa ctgcgccggc agggtcgcct
    21601 ccgaccaaaa ccacggccag gcaaggcgtg tgcagattgt cctgttggcg ttgcgccacc
    21661 gcttcggcaa tcgcctgcag gtgtttttgc gaaacttctt taccgttgat cagttgtgcc
    21721 gacatatccg tccttttgtt aaacaggtag aaaatgctga aaattatcgc attttccgtt
    21781 tgtttttaca gcagaatgcc gaaaataaat ttgccccgaa ataaaatacg gctgtttgga
    21841 cggattaaat attttttcaa atcaaaaaac tatatttttc atcttggtgt tttgaaaata
    21901 aatgttgacg catctttccc tgaactgtat attccgcttc ttcaagtcgg ggcgtagcgc
    21961 agcccggtta gcgcatctgc tttgggagca gagggtcgtg agttcgaatc ccaccgcccc
    22021 gaccaatcaa tcagcacgaa tcggcatgag cgcccgtagc tcaaccggat agagcaccga
    22081 ccttctaagt cgggggttac aggttcgatt cctgtcgggc gtgccaagaa tttgattgaa
    22141 aaccgttttt atggtggctg tagctcagtt ggtagagccc cggattgtga ttccggttgt
    22201 cgtgggttcg agccccatca gccaccccag ataaaggccc gtacttttcg gtacgggtct
    22261 tttcctgttt gcgcgtactg tccgcgccgt cgtaccggtg cggcggtatg ccgtctgaaa
    22321 gccgggccgg tcatacggaa accgcttcat acggcatttg cgccgccggc tttgcctttc
    22381 gcatatgctt ctgtatcatg tgggtttttc tgtcttttat ggtttttgtc ggaggctttg
    22441 atggttgacg cttggtctgt ccctgatttt gaatcgaaaa tctgtccgcc cgaggcgttg
    22501 gcggcgcgtt tggcgttgtt gccgcgcccg ctggtgttta ccaacggctg tttcgacatc
    22561 ctccacaggg ggcacgttac ttatctggcg caggcgcgtt cggcaggggc ggcgttggtg
    22621 ctggcgttga ataccgatgc ttcggtgcgg cgtttgggca agggcggcga ccgcccggtt
    22681 aatcctttgg agaaccgtgc cgccgttgcc gccgcgttgg aaagtgtgga tttggttact
    22741 tggtttgacg aggatacgcc ggcggcgttg attgaggcgg tcaagccgga ggtgttggtc
    22801 aagggcggcg attgggtcgt ggataagatt gtcggtgcga cggaaacgct ggcacgcggc
    22861 ggtcaggtgt tttcaattcc gtttctgcat cagacttcga caacgaagac tttggcaaaa
    22921 atccgtgcgg cagagggcgg aaaatgacgg ttttgaagcc ttcgcactgg cgggtgttgg
    22981 cggagcttgc cgacggtttg ccgcaacacg tatcgcaatt ggcgcgtgag gcggacatga
    23041 agccgcagca gctcaacggt ttttggcagc agatgccggc gcatatacgc gggctgttgc
    23101 gccaacacga cggctattgg cggctggtgc gccccttggc ggttttcgat gccgaaggtt
    23161 tgcgcgatct gggggaaagg tcgggttttc agacggcatt gaagcacgag tgcgcgtcca
    23221 gcaacgacga gatactggaa ttggcgcgga ttgcgccgga caaggcgcac aaaaccatat
    23281 gcgtgaccca cctgcaaagt aagggcaggg ggcggcaggg gcggaagtgg tcgcaccgtt
    23341 tgggcgagtg cctgatgttc agtttcggct gggcgtttga ccggccgcag tatgagttgg
    23401 gttcgctgtc gcctgttgcg gcacttgcgt gccggcgcgc tttggggtgt ttgggtttgg
    23461 aaacgcaaat caagtggcca aacgatttgg tcgtcggacg cgacaaattg ggcggcattc
    23521 tgattgaaac agtcagggcg ggcggtaaaa cggttgccgt ggtcggtatc ggcatcaatt
    23581 tcgtgctgcc caaggaagtg gaaaacgccg cttccgtgca gtcgctgttt cagacggcat
    23641 cgcggcgggg caatgccgat gccgccgtat tgctggaaac attgcttgcg gaactgggcg
    23701 cggtgttgga acaatatgcg gaagaagggt tcgcgccatt tttaaatgag tatgaaacgg
    23761 ccaaccgcga ccacggcaag gcggtattgc tgttgcgcga cggcgaaacc gtgtgcgaag
    23821 gcacggttaa aggcgtggac ggacgaggcg ttctgcactt ggaaacggca gagggcgaac
    23881 agacggtcgt cagcggcgaa atcagcctgc ggcccgacga caggtcggtt tccgtgccga
    23941 agcggcggga ttcggaacgt tttttgctgt tggacggcgg gaacagccgg ctcaagtggg
    24001 cgtgggtgga aaacggcacg ttcgcaaccg tcggcagcgc gccgtaccgc gatttgtcgc
    24061 ctttgggcgc ggagtgggcg gaaaaggcgg atggaaatgt ccgcatcgtc ggttgcgccg
    24121 tgtgcggaga atccaaaaag gcacaagtga aggaacagct cgcccgaaaa atcgagtggc
    24181 tgccgtcttc cgcacaggct ttgggcatac gcaaccacta ccgccacccc gaagaacacg
    24241 gttccgaccg ttggttcaac gccttgggca gccgccgctt cagccgcaac gcctgcgtcg
    24301 tcgtcagttg cggcacggcg gtaacggttg acgcgctcac cgatgacgga cattatctcg
    24361 gcggaaccat catgcccggc ttccacctga tgaaagaatc gctcgccgtc cgaaccgcca
    24421 acctcaaccg ccccgccggc aaacgttacc ctttcccgac cacaacgggc aacgccgtcg
    24481 caagcggcat gatggacgcg gtttgcggct cgataatgat gatgcacggc cgtttgaaag
    24541 aaaaaaacgg cgcgggcaag cctgtcgatg tcatcattac cggcggcggc gcggcgaaag
    24601 tcgccgaagc cctgccgcct gcatttttgg cggaaaatac cgtgcgcgtg gcggacaacc
    24661 tcgtcatcca cgggctgctg aacctgattg ccgccgaagg cggggaatcg gaacacgctt
    24721 aaaaaatatg ccttttttca acacggggcg gcatccgtcc gaatctgtcc ggattctgcc
    24781 gcccgacaga ctgccgcccg ggcgggcggg gtttcaaaca ttgaggaaat tctaatgaaa
    24841 tggctcttca cccttttggc ggtactcaat attgccgtat tcggcggcac ggtgggctat
    24901 aaattggccg ttaaagcggc gggcggcgtg ccggaaaacc gggccgtcga aaacacgccc
    24961 cccgcaacgc ccgccgccgg caatgcggcg gcatccgtgg aggatacggc ggcactgctc
    25021 aaaccgggcg acatcctgag cgaagaacag gcggagcagt tgcgcttgaa aaaagaagcg
    25081 gaacagaaaa aactgaggga gaaaaaacag cgtgaagaaa aagcccgccg cgaaaaactc
    25141 gccgccgaaa aggcgcaggc ggaacgtgaa aacggcgcgg cggatgcctt atgcgccgcg
    25201 caggcaagcc tcacgatgga cgaagacgac taccaccgca tcaaagggct tttgggcaaa
    25261 tggtcgcacg ttgccagcag gagcgtcgaa aaacgcaccg ccaaagccaa acctgccgac
    25321 aaaacctacc gcgtcgtcct gcccgtttcc gccgatgccg aaaatcaggc ggcggagctg
    25381 tctgccaaag gtttcaaccc cgtaccgttt gacggagcat tgagtttggg tgtcggcaac
    25441 agccgggaaa acgcccaagc cctgcaaaat cggcttgccg atgccggatt cggcggggcg
    25501 catattgtcg aacactttgc cgaagccgac aggcaggacg attctttgag cgtgtcgcgt
    25561 atgacggttt tgtttacggg cgtgaatgcc gccgatgcgg acgaaatccg taaaatcacg
    25621 tccctatacg gcaaactgaa cctcaagtct tgcaaataag tgcagaccaa aaacgccaaa
    25681 cccaccgcta gggtgggttt ggcgtttcgg ttttgagtat ctgattaaaa agggaatgta
    25741 tgtctcgtat atgccgactc caagtgtgaa agtgaattgt gagggaatac agtgtttaat
    25801 ggttcggaac ggacggcttc cgagtggttt tgattatacg ctaataataa taattatcaa
    25861 taaaaattta aaaataaatg cggatttttt gtaatgcgat gaaaaataaa caaattaatc
    25921 tgtccgccga tgtttgtgat ttgggtcggc aaactttcat tgcgccggac aaatcggggt
    25981 cggcaggcat ggacgggggc ttcgtgcctg tcggaaagaa gcctgccgtg tttgaacaac
    26041 cgtccgattt ggtgcggaca gggttgccat tcgggggtgt ctgagcgcgg attcggggat
    26101 gcaaaagcgg tgaaaccgtt gcggatgaag gcttacggat gccccgaggt atttttaaat
    26161 gtattcaaac gacaaaactc ccttctattg ccgtacttag ggcttgcgca gcagcctgcg
    26221 cgccggggag tccgggtgtt tcgttggttg cggtgtcggg gcgggcggca aggttttccg
    26281 gccggtttcc ctgttcctca cgacaaagcc gtgatgccgg ggaatggcgg tttgcccgtt
    26341 tggggccgcg atgccgtctg aagccttcag acggcatttt tgccgccttt ttcaatattc
    26401 cgccgaatgc caaaacggtt gtccgactgt cggcgtgctg gcttgggctt tggttcgcgc
    26461 ttcgaccggc ccggcttcaa atgccagccg tccggattcg acggcgagtg cgaaggcgcg
    26521 cgccatgttg acggggtcgc cgctgcggga aacggcggtg tttaacaata cgccgtcaaa
    26581 accccattcc atcacttgtg ccgcttggga aggcaaaccc aagcccgcgt cgataatcag
    26641 cggcgtgtcg ggcaggcgtt cgcgcaggat tttgagcgca taggcgtgaa ccgcccccaa
    26701 acccgtgccg atgggagccg cccacggcat caacgcctga cagcccgcat cgagcaggcg
    26761 gcggcaggca atcaggtctt cggtgcaata aggcaacact ttgaagccgt ctttaatcag
    26821 gatttccgcc gcttcgacga gttggaacac gtccggctgc aaggtgtcgt cgtcgccgat
    26881 gagttccaat tttatccaat cggtttcaaa cacttcgcgc gccatttgcg ccgtcgttac
    26941 cgcttcctgc acgctttggc agcctgccgt gttcggcagg acgggaacgc cggtttcttg
    27001 aagcagcgac caaaacccct gaccgtgcgc ctcgccgccg catcccgtgc ggcgcagcga
    27061 gacggtaatc atcgcgggcc gggcggttcg gacggattgt ttgaggattt caggggtcgg
    27121 gtaggcggct gtgccgagca gcagccgcga agggaaagtt tcgccgtaca gggtgagcat
    27181 gatggattct ttggtatggt tgtcggacgc gatgccgtct gaaagccggc ggcgtgcctt
    27241 tggggtttca gacggcattc agccgccgac gacggggcgg acgatgtcga ttttgtcgtt
    27301 ttcgtgtaaa acggtttccg tatacgcgcc tttggggatg aaaacggtgt tgaccgccac
    27361 ggcaaagggc ttttgcggcg cggtttgggc gatgaggtcg gcaacgcttg tgccgtgaag
    27421 ttcggcgggt ccgccgttta agatgatgtt catggtttct cgatgtttct gtatttatag
    27481 cggattaaca aaaaccggta cggcgttgcc ccgccttagc tcaaagagaa cgattcccta
    27541 aggtgcccaa gcaccaggcg aaccggttcc gtactatttg tactgtctgc ggcttcgccg
    27601 ccttgtcctg atttttgtta atccactata tctagccgaa ttactttatt ttttgatacg
    27661 caaccggccg gttgccgtca ttcccgcgca ggcgggaatc catttttgaa attcggaaac
    27721 tgtttttcaa atgatggttt ctcaagtttt acggcggatt cccgcctgag cgggaatgac
    27781 ggaatttgat gatatgccac atttaaagtt aagcatattt gcgataacgg gctaagtttt
    27841 gcctacacgg caattgaatt ttcttctttc ggttttatcc atcccacaaa gcctgaaacg
    27901 ctttaaccac cgcttcggga tttgccgctt cggttacggc gcggacggcg gcgagtgagg
    27961 aaacgccggt ggccagcacg gctcgggcgt tgttcaaatc gatgccgccg atggcgacga
    28021 cgggcgtgcc gcgagcctgt tccacatatt cgcgcaattt gtccaagcct tgcggggcgg
    28081 tgggcatttg tttggtcgtg gtctggaaaa tcgcgccgct ggcgatgtag ccggggtgta
    28141 cggacagggc gcggtcgagt tcggcgacgg agtgcgtact caaacccaaa cgcaaaccgg
    28201 cggcggcgat ggcggcaagg tcggcggtgt ccatgtcttc ctgcccgaga tgtacgccgt
    28261 atgcgcccgc ttcgattgcc tcgcgccagt ggtcgttgat gaaaagctgt gtgcggctgc
    28321 cttgacaggc ggcgacgcag cgggcgattt cgcgtttcaa ttcgttgccg tgcaaggttt
    28381 tacagcgcag ttgcaccgtg tcggcacctg ctttgaccat gcgccccacc caatcggcgg
    28441 tgggaacgac ggcgtagaat ttaagtaggg attttagggg cgggaaggtc atagggtgtc
    28501 ctttcgggcg gctttaatct tgtcttccga tatacgccaa accgctttct tcatcacgtt
    28561 cgggcgcgtc ttttccgtca aacagtgcca ctgccaatct gacggcggcg gcggttacgg
    28621 cgggggaaat cataaagccg tgccggaaaa ggccgttgat ttcgatgagg cggcgttcgc
    28681 ggctgtagcg gatttcgggg ttgtggtggt tgagcgtggg gcgcaggccg gtggcgattt
    28741 cgaggatgtc ggcttcgccg aaggcggggt ggacggcata gagcgcggat aagagttcca
    28801 gcccggaacg tacgctggtg ggggcttggc tttcgctttc gatttgggtc gcgccgatga
    28861 cgaagacgtg gttttctttc ggggcgatgt agagcggata gcgcgggtgc agcaggcgca
    28921 cggggcggtt gagcgtgatt tcgggcgtgt aaacccgcgc cacttcgccg cgtatgccgc
    28981 gcaaggtgct ggtgtgctcg ggggattggt tccacgcggt tttcgcgccg tagccccggc
    29041 agtcgattac ccagtcgtat tgggcttgca ggtcttgggg ggcgcattcg tgttcccaat
    29101 ggcaagggac gttcagttcg tccaaagcgt cggcaagtgc agacaatatt tgccgcccgt
    29161 cgagctggcc ttcggtcggc aggtagatgc cgtctgaaaa acgtccgccg agttgcggtt
    29221 cgcgttcggc gatttcatcg gcgcgccaac ggacgatttc gtcatccgct acgccgccgc
    29281 gtttgagatg gcggacgaac tcgctggata atggcttgtc ctgcccgtgc cacacaatca
    29341 ggctgccgtt ttcctgcatc atcgtgtgcg tgttcagacg gcatcggatg ccgcgccaaa
    29401 gcggaatgct ctgcctgccc agcctgatga cttcgggcgt tgcctcgacc gcttccgccg
    29461 caggcgcgag catcgcggcg gcaacatagg cggcggcgtg ttcgccttgg cgggtgcctt
    29521 tgtcgaaaag ttcaatctga taaccttgtt ctgcaagctg caatgcggtc agccttccgg
    29581 aaaggccgcc tccgaggacg gcgatacggg tcatgacggg ttcctttgta aagattgggt
    29641 ttttttaaag aaaaggcgta ccgatacggt ggcaatggca acggcagaca ttacgggggc
    29701 ggtcagaccg atgctgcttt cccaaccgga cgagagcagg aagcggtaga ggatgaagcc
    29761 tgccagccac agaaccagtc cggcaaagtc aaagccttca atctcctcac gccgttttaa
    29821 gacgaaaaag tcggcaatca aaaccgccgc catcggcgca aataccgagc cgataagcag
    29881 caggaagttt ttatattcgg taacgggcag catgacggca agcaccgtgc cgatcagggt
    29941 aacgccgaca gcgacgggta tttccgcaaa acgcgcggaa atgttgttcg cactcgcgcc
    30001 ggcggaatag gtatcgagaa acgttgtggt aacggtggag aggacgactg ccagaatgcc
    30061 cgttatgccc aagcccgcgc ccaacaggat tttcgccacg tcggtttctc cggtaaacag
    30121 agccgccgcc aaacccaagg catacatcca gcagcccgtc agcgtatagg cgagcgttgc
    30181 cgtcagggtt gccgcaaacg ggcggcgtgc ttggcgcgtg tagtcggcgg ccagcggcag
    30241 ccaggaaagc ggcatgacgg cggacagttt gactgccgtt ccgaaggtca tgccgtctga
    30301 aacggcgggc gcggcgtttg tgccggacga agcgaacact tcgacgctca accacaacac
    30361 ggcaagcagc atcagcagca tcgaaacggt tttcagcccg cccgttctgc gtgcgccgaa
    30421 aaccagccac agcacgatca gtgcgccgtt tgccaatgcc caccagacaa aggattcgcc
    30481 gtcccacaac actttgccca aagcggagct gaccgttgcg ccgacgtaaa tcatcaccgc
    30541 cgtccagccg gccagttgca gcatattcgc cacggaaaac agcactgaac cgcatttgcc
    30601 gaacgacagg cgcacacttt ccatcgagct gcgtccggtc agtgcgccga tatacgctgc
    30661 cgcaaaaaac agcgcgccgc cgacggcatg acccaaaagc agggccgcca gaccgcgctg
    30721 ccagcccaag ggggcgagca gcgtacccgt gctgatttcg gcaatcgata ccgccgcgcc
    30781 gaaccaaacc agcccgatgg cggcggaaga tgaaggagag gaggcattgc ccgacatatg
    30841 acgtccttta aaaagaagga aggtggtcgg ggaagtgcgg aaaagggaac ggaagcccga
    30901 aagggcgggg aggttttgtt tcctacgcag gcattacccc gacaggttct acggattttg
    30961 ctttcgcaat ctcagccgcc cggcgggcgg cactccgaca agaaggcttt aagtttacac
    31021 aaagcaacgt gcgttggcaa aattcttaca ttttcgacgg cacggcgcat ttccataaca
    31081 aatgccgtat aatcaagccc ctgatttttt atgcgccgac actatgcatc cgacctattc
    31141 cgccgtacag gcgcggctgc tcgaagccaa ccgcctttcc cccgaactgc tcgccaaaag
    31201 cctgtgcatc atcggcgcgc accacgtcga ttatgccgat atctactgcc agcgcaccgc
    31261 ttatgaaagc tggcatttgg aagagggcat cgtcaaatcg ggcagcttcc aaatcgatca
    31321 gggtgtgggc gtgcgcgccg tttcgggcga caaaaccgcc tttgcctacg ccgacagcct
    31381 gtgcatcgat tcgataaacc gttccgcccg cgccgtccgc gcgattgggg cggcaggcgg
    31441 caaggtgtcc gccaaaatgc cgtctgaaac gcgcggcaag ccggtttgtt ccgcatccga
    31501 ccccattgcc ggcctcgatt ccgccgccaa agtcgcgctg ttgaacaaag tggaagcaat
    31561 cgccaaagcc gccgatccgc gcatcgtgca agtgatggcc ggtttgacct gcgaatacga
    31621 tatggtttac ctcgcccgtc tggacggcaa acacgccgcc gacatccgcc cgatggtgcg
    31681 cctgaacgtt accgtcatcg ccaaacaggg cgaacgccgc gaacagggcg gcgcgggcgg
    31741 cggaggacgc tacgacttgg cttatttcga tgaaaccttg gtgcggcagt ttgtcgatgc
    31801 cgccgtcaaa caagccctga ccaatctcga atcccgtcct gcgcctgccg gcgagatgac
    31861 cgtcgttttg ggcaacggtt ggccgggcgt gttgctgcac gaagcggtcg gacacggttt
    31921 ggaaggcgat ttcaaccgca aggggaccag cgtcttttcc ggccggatcg gcgaacgcgt
    31981 cgccgccaaa ggcgttaccg tcgtcgatca gggcgatatt gccggccggc gcggttcgct
    32041 caacatcgac gacgaaggca acgaaacacg ccgcaccgta ttgattgaag acggcatctt
    32101 ggtcggctat atgcaggacg aaaccaacgc ccgcctgacg ggtacgcaat cgaccggcaa
    32161 cggccgccgc caaagctacg cctccgtccc gatgccgcgt atgaccaaca cctttatgga
    32221 aaacggcagc tacgagccgg aagaaatcat cgcgtccatc gacaagggca tttacgccgt
    32281 caacttcggc ggcggacagg tggacatcac cagcgggaaa tttgtcttcg gcgcgtccga
    32341 agcgtggtgg gtggaaggcg gcaggctgca ataccccgtc aaaggcgcga ccattatcgg
    32401 caacggcccc gaagtgctga aacacgtttc gatgataggc aacgataccg cgctcgacag
    32461 cggtgtcggc gtgtgcggca aagaagggca gagcgtcccc gtcggcgtgg ggcagccgac
    32521 cctgcgtatc gatgccgggc tgaccgtcgg cggcagcgca atctgacgcg gaatccggca
    32581 cgatgccgtc tgaaaggttt tcagacggca tcgccttatt cttttttccg atgtttgata
    32641 ttcctaagca ttctttaaca ttatcttaca agttcattaa aataccgcct ttcgttaagc
    32701 tgatttctga tgaactggcc ttatttaatc gatgccgtac ccaaattcgc cgatgcggca
    32761 aagctgacgc tggaattgtc cgtttacggc gttgttttgt cgttgctgtt cggcctgccc
    32821 gtcgccgtgg taacggcata ccgcatccgc cccttctacg cattggcgcg cgcctatatc
    32881 gagctgtcgc gcaatacgcc cctgctgatc caattgtttt tcctatatta cggtctgccg
    32941 aaaatgggca tcaaatggga cggtttcacc tgcggcgtaa tcgcgctggt tttcttgggc
    33001 gcaagctata tggcggaagc tgtccgcgcc ggcatcctcg ccgtccccaa agggcaggtc
    33061 ggagcgggca aggcaatcgg tttgagccgc tttcaagtgt tccgctatgt cgaattgccg
    33121 caggtttggg cggtcgccgt tcccgccatc ggcgcaaata tattgttttt aatgaaagaa
    33181 acatccgtcg tcagcacggt cggcattgcg gaattgttat ttgttaccaa agacgtcatc
    33241 ggtatggact acaaaaccaa tgaagccttg ttcctgctgt ttgccgctta tttgattatc
    33301 ctgctgcctg tttctttgtt ggcgcggcgg attgaaaacc gcgtacggag tgcgaaatat
    33361 ggcgtttgaa tggctgtttg aaggaaaaaa tgcggcacgg ttgggcgaag gcctgctctt
    33421 aacggcacaa atttctttaa tctctgttgc ggcttcttgc gtattgggca cgctgttcgg
    33481 cttggttttg cgttcgcgca accggcttgt ccgctttgtc ggacggtttt atctcgaaac
    33541 catccgaatc gtgccgattt tggtgtggct gttcggcctg tatttcggcc tatccgtctg
    33601 gacaggcatc catatcggcg gattttgggt ctgcgtctgg gtattttccc tgtggggcgt
    33661 tgccgaaatg ggcgacttgg tgcgcggcgc actggaatcg attgaaaaac acc
//
LOCUS       DAXFHF010000019        32974 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXFHF010000019 DAXFHF010000000
VERSION     DAXFHF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32974
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.019"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            164..595
                     /locus_tag="ACFIRM_000794"
     CDS             164..595
                     /locus_tag="ACFIRM_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3028462.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
     gene            787..2826
                     /locus_tag="ACFIRM_000795"
     CDS             787..2826
                     /locus_tag="ACFIRM_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC3028463.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASNQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            2841..3380
                     /locus_tag="ACFIRM_000796"
     CDS             2841..3380
                     /locus_tag="ACFIRM_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028464.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            3502..4056
                     /locus_tag="ACFIRM_000797"
     CDS             3502..4056
                     /locus_tag="ACFIRM_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC3028465.1"
                     /translation="MKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDRTINCKR
                     ADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYAMQEWAR
                     QSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELEQMAKYY
                     NVGGIGIYNSFVHLDTGRVRHWRG"
     gene            4320..4769
                     /locus_tag="ACFIRM_000798"
     CDS             4320..4769
                     /locus_tag="ACFIRM_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC3028466.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(4790..5113)
                     /locus_tag="ACFIRM_000799"
     CDS             complement(4790..5113)
                     /locus_tag="ACFIRM_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028467.1"
                     /translation="MTYGREGHSGKLPKGQPSNSCFKSNKNKKFGLLKNCIFLLMLTI
                     DMSIDKKTILLKAKSLRGLTLCFTIIQELLGKLNRRFDRGLIFQAMSWKTKNGEARKL
                     PTILK"
     gene            5954..6469
                     /locus_tag="ACFIRM_000800"
     CDS             5954..6469
                     /locus_tag="ACFIRM_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC3028468.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKNPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            6479..7060
                     /locus_tag="ACFIRM_000801"
     CDS             6479..7060
                     /locus_tag="ACFIRM_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028469.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            7286..7594
                     /locus_tag="ACFIRM_000802"
     CDS             7286..7594
                     /locus_tag="ACFIRM_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028470.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARFSAIDTKINAIYWFIGITTTVAVAAAKFL
                     "
     gene            7833..8111
                     /locus_tag="ACFIRM_000803"
     CDS             7833..8111
                     /locus_tag="ACFIRM_000803"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC3028471.1"
                     /translation="MTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAREA
                     AQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            8113..8604
                     /locus_tag="ACFIRM_000804"
     CDS             8113..8604
                     /locus_tag="ACFIRM_000804"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3028472.1"
                     /translation="MPLPDFRIRLLDAADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRISKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            8700..11183
                     /locus_tag="ACFIRM_000805"
     CDS             8700..11183
                     /locus_tag="ACFIRM_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC3028473.1"
                     /translation="MYNPVSKIDELLPDFAELDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETEPPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKASGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEGSVEVQLAKRFVDRQD"
     gene            11431..11688
                     /locus_tag="ACFIRM_000806"
     CDS             11431..11688
                     /locus_tag="ACFIRM_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028474.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            12241..12465
                     /locus_tag="ACFIRM_000807"
     CDS             12241..12465
                     /locus_tag="ACFIRM_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028475.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGSTFD"
     gene            12536..13657
                     /locus_tag="ACFIRM_000808"
     CDS             12536..13657
                     /locus_tag="ACFIRM_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC3028476.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRILDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTNGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            13734..14075
                     /locus_tag="ACFIRM_000809"
     CDS             13734..14075
                     /locus_tag="ACFIRM_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028477.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            14311..14703
                     /locus_tag="ACFIRM_000810"
     CDS             14311..14703
                     /locus_tag="ACFIRM_000810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028478.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGEGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGGIAVFIR"
     gene            14684..14893
                     /locus_tag="ACFIRM_000811"
     CDS             14684..14893
                     /locus_tag="ACFIRM_000811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028479.1"
                     /translation="MQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGFSLSDVLTD
                     GCRHVLREVATGWLNREILPELYNV"
     gene            14928..15500
                     /locus_tag="ACFIRM_000812"
     CDS             14928..15500
                     /locus_tag="ACFIRM_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028480.1"
                     /translation="MPITCGLLAAGAETLLEIVMSIKVDNILLIEGTKESVAEAAKLI
                     LFRSPVHPEHWAFDFNRILPLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIE
                     ICFLTGLAPPFGIYRKLAEIFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMA
                     DIRKFGIAEFDIWYEGLSDRLESDGYLVED"
     gene            15651..15890
                     /locus_tag="ACFIRM_000813"
     CDS             15651..15890
                     /locus_tag="ACFIRM_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028481.1"
                     /translation="MPDNMKSKTKVPTAVLASWAAILDDIAKAAIIGIGVVVWSNGYS
                     EEMKNLAMAGLVLLSFEMLYVSFLIRIHISKLEDK"
     gene            16203..16982
                     /locus_tag="ACFIRM_000814"
     CDS             16203..16982
                     /locus_tag="ACFIRM_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028482.1"
                     /translation="MPNWCGNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDEPFAKRIAENIRQYGHPNWYDWR
                     LANWGCKWDVNPDCTFVTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEE
                     GMWFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            17071..17535
                     /locus_tag="ACFIRM_000815"
     CDS             17071..17535
                     /locus_tag="ACFIRM_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028483.1"
                     /translation="MIENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLYTAVRLLNENQGILHSISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESRLDACLK"
     gene            17939..18250
                     /locus_tag="ACFIRM_000816"
     CDS             17939..18250
                     /locus_tag="ACFIRM_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC3028484.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKDPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            18252..18701
                     /locus_tag="ACFIRM_000817"
     CDS             18252..18701
                     /locus_tag="ACFIRM_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC3028485.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            18816..19886
                     /locus_tag="ACFIRM_000818"
     CDS             18816..19886
                     /locus_tag="ACFIRM_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028486.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMVSWFKS"
     gene            19939..20553
                     /locus_tag="ACFIRM_000819"
     CDS             19939..20553
                     /locus_tag="ACFIRM_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028487.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQVYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            20620..20970
                     /gene="exp2"
                     /locus_tag="ACFIRM_000820"
     CDS             20620..20970
                     /gene="exp2"
                     /locus_tag="ACFIRM_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Exp2"
                     /protein_id="HFC3028488.1"
                     /translation="MKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEATLNLFVEGFW
                     IIGLLQALAFTTIVDYCTESKHTNMLNNPACNDEDWDNDMLNNPACNDEDWDNDMLNN
                     PAYSWSMFNIHHSD"
     gene            21174..21752
                     /locus_tag="ACFIRM_000821"
     CDS             21174..21752
                     /locus_tag="ACFIRM_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC3028489.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVGNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPSVTFSVLTFFLNKLFRNMKLGSDADVVT
                     NNDWLNDFWEDRRRHCPSYSHHPSNIWHLSED"
     gene            complement(21856..22020)
                     /locus_tag="ACFIRM_000822"
     CDS             complement(21856..22020)
                     /locus_tag="ACFIRM_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028490.1"
                     /translation="MSIAHIVIEIEESSTLDDCNVRLSFSGGTELQSDEAKTVFYKTL
                     EAIEGVLGDD"
     gene            complement(22036..22479)
                     /locus_tag="ACFIRM_000823"
     CDS             complement(22036..22479)
                     /locus_tag="ACFIRM_000823"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC3028491.1"
                     /translation="MGPLLPDAHTIISPFGKRVHPILKKVINHKGEDYRGSINTPLLA
                     PFSGRVVESRYQANTVNGKTVGWGHTVVIEAPNGVRYRASHMNGASPLRFGETVSKGQ
                     QIGNLGASGGVTGPHLDAMLYSPSGKILPVSAYRGKTLNEIGKIK"
     gene            complement(22542..25454)
                     /locus_tag="ACFIRM_000824"
     CDS             complement(22542..25454)
                     /locus_tag="ACFIRM_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC3028492.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMHQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNSRFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGENRYQGRGDSFGNVSSTSTVSMSDVYTQAAD
                     RSTTAAKQYAASSVEQTTLGNTQTLAFGIQTVGQNGANYAVTNGMSSSTAKALDQILQ
                     SSKDIAQRNGMGNDVQTVSSIALDLGAQLAGGVTAGSKGAGPQASATVSGGASIRGEG
                     KSSNKLSHDISETAKALKSQGVSITRSMLENIGQSNAFTQALTDSSSLASQAQASYNK
                     AHQASISAQQSYNEAQNYREAASRTAQSGATVSIDNAKAIREYADQNGLAYSQVYNNP
                     SIVAAAAMNAAVTQASNLRQQAEGTRFDSTGPISPDDRPTNNVAAAYSSYKSGIDGQK
                     SSTFATIQAQADGNGVNRNEARANIDGWQQKLEQGSKDAKNFNQKLFDKGMKKQEENT
                     NKLHAQGEDTFKHVNTFVMGATQTFSSDKAYVQAQNKYGKTEGRPGSGSRNYRPKK"
     gene            complement(25461..26972)
                     /locus_tag="ACFIRM_000825"
     CDS             complement(25461..26972)
                     /locus_tag="ACFIRM_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC3028493.1"
                     /translation="MKFHIFRFPKPAKVPVVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGTWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(27144..27920)
                     /locus_tag="ACFIRM_000826"
     CDS             complement(27144..27920)
                     /locus_tag="ACFIRM_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC3028494.1"
                     /translation="MAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(27932..28357)
                     /locus_tag="ACFIRM_000827"
     CDS             complement(27932..28357)
                     /locus_tag="ACFIRM_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028495.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(28344..29900)
                     /gene="traN"
                     /locus_tag="ACFIRM_000828"
     CDS             complement(28344..29900)
                     /gene="traN"
                     /locus_tag="ACFIRM_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC3028496.1"
                     /translation="MICHFMLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVT
                     KDCWEYKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDV
                     DLRTLHQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKTINGV
                     PVTRDCWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTCQAREKQFEC
                     TEKGETTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQCSIKLDGAL
                     DSVFGGDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGT
                     ALSAAGGITGTSQIGALGFSAAGQQGMGVIVGFNSAVFAAAIAVIAIQQWLKCPQSEI
                     LVAMKRKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGKEQLKRGWGT
                     PENPKCEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKIREASVNGKN
                     LEVKNYYEYQ"
     gene            complement(29924..31168)
                     /locus_tag="ACFIRM_000829"
     CDS             complement(29924..31168)
                     /locus_tag="ACFIRM_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028497.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(31165..31917)
                     /gene="trbC"
                     /locus_tag="ACFIRM_000830"
     CDS             complement(31165..31917)
                     /gene="trbC"
                     /locus_tag="ACFIRM_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC3028498.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(31932..>32974)
                     /locus_tag="ACFIRM_000831"
     CDS             complement(31932..>32974)
                     /locus_tag="ACFIRM_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC3028499.1"
                     /translation="EAVPTAPIPNKLTCQGKMLNPINDICWSCMFPIKIGGKINLNMD
                     NQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPTHLVEVVRTPFCMVSLGGISLKNQKAD
                     TAETTGKADVSNKIQNLFGGSHKRGRNEQHDQHNAFYQVHWYKNPVVFWLEALLDNDC
                     MEKGTLDIGWMTELDPTWKYPSLAVYQAPDSALFANVVAQAACAADCVKAAAGFPFNK
                     LYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMAARIQTKMHRDLVAWRTSGRDALCGYK
                     LNPVMDKQNYKMAMTYPVAARKFRGRCCEPIGRSTLVRDSGKAFPYKGNQFAFQLFRK
                     RDCCGAYKFKTN"
BASE COUNT         8755 a         7477 c         7480 g         9262 t
ORIGIN      
        1 cgtgggcatt ttagctagat atagcataag gtttaatttg taatagttgc tagttgacaa
       61 gaggaattta tttagttatg ataatagaac ccttaatctc atattgggtt attttaccct
      121 gtcttttctg tcatattttt aaatcctttt tttggagaaa aatatgtcag ttcaactttt
      181 tgttcgtggc aatctcggtg ctgaatttaa tctgcgtcct gtagcaagcc ctaaaaacga
      241 tgaagcctca atcgtactga atttttcagt tgcctcacct aatttcaaac gcaatgccga
      301 aggtaaatca gaagtggttt caacggagtg gatttcttgc gaatactgga atcgtgatgc
      361 tgcccatctc cataaaatat tacaggtcgg tatgccggtt ttcgttaccg gtgaagaacg
      421 ttacgagtct tataccaatc gtgacggtat tgaagttcgc gttcgtaaat tgcgtgttca
      481 agatgtattc attgttccaa ctgagcgtgt agaagctatc aagttgcgtc ctaaacgtga
      541 gcagtctcag gacaataatc acgttgaaga cgctgatgat gacatcccat tgtaaatata
      601 tggccacgaa gaatacttcc ttttttcgtg aaatgaactg ttggcttgct gcattatggg
      661 ctgactttaa ggatatgttc attgttaccc ttatttatgg tggcttaatt gctttggcaa
      721 tttgggcttt tttgactatc gcatcttggc ttgttttgca gctgaagcaa agcttgggat
      781 tttaaaatgc gtttgttttt atgtgaaaaa ccatctcaag ctaaggacat cggtaaagtg
      841 cttggtgttc ttggcgggag gcatgatggt tattttcaga atggagacac tattgttacc
      901 tgggcatttg gtcatatcct gacacaggcc gcgccagatg cttatggcga acaatatgct
      961 gactttggaa atatctccgc attgcccatt ctccctgaag tttggcagat gcaggtaccg
     1021 ccgaaggtat cgaagcagtt taaagtcatt aaaagcttgt tggccaaagc agatgaagta
     1081 gtgattgcta ctgatgcaga ccgtgaaggg gaagttattg cccgtgaaat attggactac
     1141 tgtggctacc gtaaaaagat ttcccgattt tggacgagtg gcctagatac ccaatcggta
     1201 aaaaaagcct tatcttcaat aatgccgggt aaggataaag aaaacctata tcaggccggt
     1261 ttggcgcgcg gccgcgccga ctggctggta ggtatgaacc tgagtcgtgc ctacacagct
     1321 gcttattctg ccggatatgg ccgagaacat attttgtcga tcggacggat tcaaacaccg
     1381 actttaaagc ttgtagtcca aagggataaa gagatcgata ctttcgtgcc ttacaactac
     1441 tacacccttt cagtagattt tgagacagca gacaagtcag tgtttactac actgtggcag
     1501 gttcctgaaa actggcgtaa tactgacgga tactgtacca accttgaggc tgtaaaggca
     1561 cttgtcaaac agcttgagaa atctacaggc aaggttatta cctcaaataa agagcgtaaa
     1621 agcaccccgc ccccgttacc gtacagcctt tcggctttgc aaaaagaagc cggtaaaaaa
     1681 cttggcctat cggcttccaa agtgctcaaa attgcacaaa ccctctacga aaaatacaag
     1741 atcacgtcat atccccgcac cccttgcaga tacttgccaa aatcgcagat gagtgaagtg
     1801 aaagatgtat ttgccgcgat acagactatc gataaatcca tgctttcagt gattgaaaaa
     1861 gctgatcctg aacgtcaatc aagggtatgg aacgataacc aggtaaacaa acattcccat
     1921 catgcgatta ttccgaccaa ggccagtaat tttgatttgt cagtcctgga taagaatgaa
     1981 ttaacggtgt accgaatgat acgagaccgt tatatcgcac agttttatcc agattttgaa
     2041 tatgactcta ctgtagttga ggttgaggca tgttcacatt tgtttaaagc ctccaaccaa
     2101 tcaccggtta tttccggttg gaaggttctt ttgggcaagg acgtgtttga aggcgatcaa
     2161 atcgatgaag gtcctgctct accacatctg aaagtcaacg atgaggtcga cacgctttcc
     2221 ttcaatcccg aaactaaaaa gaccacgccg ccaccgcgct ttacagaggc ttctctgctc
     2281 gatgagatgc agactttgaa ggactttttg aagaatgtag aagacgaaca gatcagaaaa
     2341 attctcaaaa gtacggaagg cttgggaacg gaagccactc gcgctaccat tatcgatcgt
     2401 ttgtttgaaa tgggttatat ggaaaagaaa cggagcaaaa tctatgcgac agaaaaaggc
     2461 cgtaatctga ttgcccgtat tcccactatg attgccgatc cgataacgac agccaaatgg
     2521 gagctggctt tggctgggat agaagccggc aaattaactt tagctgaatt tatggcttat
     2581 cagcagaaag tgatcacaga gctggttggg caggcgaaaa aggatgctgt aggaaaagct
     2641 cgtccgccaa aacagacgga ttcagctggt acaaaaaaac aggcggtcgc tcgaaatgaa
     2701 gacgatgtct gtccgacctg taaggaaggc cgtctgagac agagaggatt caaggaaaac
     2761 cctgataagc ggtattgggg gtgtagtagg tttcccgaat gtaaacactt cgaatgggtc
     2821 aaataaaaaa ggactttgaa atgaagaagc tggtaaaaaa attatccgat tcaatattcg
     2881 gaaatacttg tatagagtat gtgctagatg aaaaccataa ttacaaggag caatacaacg
     2941 ctaataaaga tttattcatt tacatcaatg gcattcaagt agggagtatt tcacctggcg
     3001 aattgcaaga attgcaatat agagtgtttt attctagtag cctctggtgg agtatcttta
     3061 aatctatact aggattttta ttgaaacccg caataattgc gcttactgcc ctgccgctgg
     3121 gggtcttgct tttactgtgg ttgaacaccg acaatcctga agcgtttcat cgattattca
     3181 tgatgttaca ggatccggca cattttgcaa aagcaatatc cggacttttt cgtctgtttt
     3241 attgtatatc attctttggc acttttgtta aattggtttt taaccaagat tcaatttttt
     3301 caatttttaa taaagaactt tctaagaata tacagagtaa caaaacgtgc tcgatggata
     3361 ttgtggctgt cagagattga ggaagctcaa agatgttgcc ctctaccagc atactggaaa
     3421 gtttgcatat caatactgag tgttgcttaa aatttgattg gagtcattgg tttggaattt
     3481 cagatggata gaagaaattt tttgaaaaag tttgtcggcg ttggcgccct atatacgacc
     3541 ggtgctgccg gtttgttatt gcctaaagat gtagtagcgg caaccagtat ggctgatttt
     3601 tggtcacgag accgtacaat caattgcaaa cgggcagata ctggcgagaa acatgaaatc
     3661 cgtttttttc agcaacaaaa tggatatgat cttgatgctt accgcaatgc ctgttggctg
     3721 atgcgtgatg cgaaagatgg aaatgccatg gttcaaattg acgtagggct tttaaacctg
     3781 atgtatgcaa tgcaggagtg ggcacggcaa tcaggacggt caaaccctgt gattacgatc
     3841 aatagtgcat atagaacgcc aagacgaaat gcgactattg agggtgctgc tagaaactca
     3901 cttcatatgc gtggtaaagc agttgacttt actatgaggg gggtgggcat tggcgagctt
     3961 gagcaaatgg ctaaatacta caatgtgggc ggtattggta tctataatag ttttgtccat
     4021 ttggataccg gcagggttcg gcattggcgc ggttaaagat tttacgaaaa aatattgaca
     4081 tacgaaatta tcgggaatat attgtaattg tttttgtagt ttgtgacttt tcttaaaagt
     4141 ctcgtcattt agccgttcat gaatgagatt cgattcgtcg aatttacggt acttcttttt
     4201 cagccagcct ttaaggcttc taagtcagtt cggctaccgt gaaagccgca acttaaccct
     4261 ttggggatat actgtcccct ttgggattga tatatcccct gtttttttgg agatatatca
     4321 tgactcaatc taatcaaaac tactttaacc tgactgcaga tggtatcggt tttttaaacc
     4381 ggccacgtga agttcaagga aagagaaaat cttccaacta ttatgcttgt acgatccaag
     4441 cctcccgagg tgataatggg gagaaaaccc gtttcgatct tcgtatcgtc ggaggtcaag
     4501 cgaaagagct ctttgctcgc cttttggaag aatttccgca attgctgtct aaaaattacc
     4561 gtgaacgccc gactgtcgtg gtcggtttcc gtgccggtga tattcaacct ttaaaatttg
     4621 aaactaacaa tcgtgagacg ggcgaagttt tagaaacttt aagtatcgac ggtcggttgt
     4681 tgaaattcaa atttattaaa gtggatggcg atatgtggta tcaatcatcc tctcaagatg
     4741 aaacccaaaa taatcctgat gcttcttaga aagctattga atattttgat tatttaagaa
     4801 tggtaggaag ctttcgggct tccccgttct tagttttcca gctcattgct tggaaaatta
     4861 agcctctgtc gaaacggcgg tttaattttc caagcaactc ctgaattata gtgaagcaaa
     4921 gggtaagtcc cctcaaagac ttggctttca gcaatattgt ttttttatcg atgctcatat
     4981 ctattgtgag catcagtaaa aaaatacagt tctttaacaa tccgaatttt ttatttttgt
     5041 tgcttttgaa gcaactgttt gaaggctgcc ctttgggcag cttacctgaa tgcccctccc
     5101 taccataggt cattcaggta agcttccccg gttgacagac cgtttcttct gtctgcgtat
     5161 agccgtataa ccagtgcgcg caattattcg taattgttac ggttttatta ctatccagac
     5221 cattatctga ttacttcgtc ggtgcggact aggtaatcat tagccagggt aatccccatt
     5281 tgaacctgct ttgcaggttc tctaagacgt tttgatattt gcagaacggc tcagagaatc
     5341 tgtatttcaa gagttttata cagctgacag actgtttttt ctgtcattgc atagccgttg
     5401 attgcaagcc tgtttcaggt acggtacagc tgcttttttc tgaagcagat ttcttttaac
     5461 ccatcggggt acacttcccc gaatgggcat ggtgtaccct ttattttgga gtattaccat
     5521 gaatctaaat ctatcttttc accgttcagg tgtcatcctt cccccaaatt tactaccgga
     5581 aagaatacgt atcggtgaca tcctcaccca ccgacatact catcggaaag ttgtcgtctc
     5641 atgcatacaa gaacatcatt tgttacttgt tgatgcggat ggccgtattt cgaaaatacg
     5701 aacccaaaag gcggtcaatc ggtattgtag gagcgtcaac gatgttcata gccataaaaa
     5761 cgcttccgta gcgttgaata tggctattcg ggcattggat aacgacaaac gtatttttac
     5821 aagattggga ctgcgtatgt cccaaaaagt atatttggat aagatttaca gtgctattaa
     5881 gcgctaattt gttttttccc aaccacggga tatctgcccg cggccggtat cccttttctt
     5941 ttggagtgat accatgtcca tcaatctttc cggcaccttg acggtgcaag tcatacaggg
     6001 tcgccgaggc cctttcaaaa cagctaaatt agctacatca attggaacat ttgatgtaaa
     6061 aaacccaata ttgaaacagt ttgagcctgg tgaataccaa ggaaacttca ttattgaaca
     6121 gctcaaagtt aaggcttatg agtggggtgg tggcgccacc tcttacttag atgcagctct
     6181 tgactggcaa catctggcgg aatttgcgca ggaattttct tccgggtcgg attctccgca
     6241 agatgatcca tccgatccgt tcgatccggt tcctgaaaac ggcatccggt tgcctgaata
     6301 cgcggaaact gcggatcagg tcggcgaaat gatcgcccaa aaccgggagc atatcagttt
     6361 gggtgagtat atcctttcag accgtgacga gctgaacata gcccgttcaa tgatgaagga
     6421 ggcagggtat tcctatcggc cccagtcgca gacttggtgt ctgcaatagg aggttggaat
     6481 gctttacctg acttcccgca atgatttgat ggccgatgcc ttcttcattt ggaaccgaca
     6541 gttgatgttt gcttctctgc acggccgcga tgcagacatg ttgtccttcc aagctcaact
     6601 gcaagtaagt aatgaacggc ttggttttag acggccggag gaggttttaa gctgtccgcg
     6661 cctgacgact gccgagcatt gcttgaatct gagcaaatac atgacgaaat atcagactct
     6721 taattatggt tcggtcacgc acatgttttt gtattcggat attctgattc aacctaactt
     6781 cgatagtaaa acaggttggg ttatgttgga tgatgttacc gcagacttgg acaaggcagc
     6841 ttggcaactg gtgcggcaac tctccgatat accgttattg gaacattggc agaatgctgt
     6901 tttatcggta cttgaggctg ataaatccat catgcggttt actcctcgta tagatgaaga
     6961 gtacgcagta gttggagttc aagcggtacg agttgatatc ccggaagatt ttgatgtgcg
     7021 tttgaccgct atgctacaaa gcggccgctt acatacttaa tctgaagccc ctagcgggct
     7081 ttttcagacg gccttgcaaa ggctctctga aaaagtctgt tacataagag ttttacacag
     7141 atgacggact gtatattccg ttgatggata ttgctgctga ggttatccgg ccgtattcgt
     7201 atggttgcgg catttttcaa ttttaccaac cctgtgggga tatacgtccc atggggaggc
     7261 atattcccaa acttaggagg attttatgcc tactctatct gaaaagcatg aggtactgtc
     7321 cggcttgaac cgtcaggatg cagctgctat agtagcctta atcgatttga aaacggagga
     7381 cgacatggaa aaggttttga acaaactgga tttcatgacc cagaacttcg atgcccgact
     7441 ggacgctttt gaaaaaagtg tagatgcccg tatcagctca tttgaaaaaa gtgtagatgc
     7501 acggtttagc gcaatcgata ctaaaatcaa tgcaatttat tggtttatcg gtatcacgac
     7561 taccgttgcc gttgctgccg cgaagttcct gtaacattaa acccgttccg ttaaacggag
     7621 ctttttatct gtaaccttca atcccaccat gttccggcat ccggtgggat ttttatcatt
     7681 tctcactcaa ccctatgggg atatacatcc ctatggagat cgtatatctc cgttactttg
     7741 gagcatgtga tgctttctac aaaataccct ttcatatctt gttttgtacg gtaatcagcc
     7801 gtacaatcta gaaataattt aggagtatgt tcatgactgc cacatatgcc gattgccgtt
     7861 ttgaagcaag aatcacttca gatacccgtg ctttacttaa acacgctgcg gaacttcaag
     7921 gacgcagtat gtccgaattt gtcatcagtg ctgcccgcga ggctgcccaa aaagcagttg
     7981 ccgatgcgga gattctgatg ctgtccgaac gtgaccaact tcagtttgcc caagccctgc
     8041 tcaatccgtc caaaccgaat ggggcattga gcagagcatt ggaaaaacat gacgaactgt
     8101 tcgggagata aaatgccgtt accggacttc cgaatccgcc tccttgatgc tgccgatgac
     8161 cgcaatgcat ttgagcgccc ttccgaacct ttaaaccgct atctgcaaaa acaggtttcg
     8221 caagacgtaa aacggaaagt cgctacttgt tttgtcgcgg ttacatccga cggtcaaatt
     8281 cttggctact atacattggc tgcaacagga gttgccctaa gtaatatacc cgccggtttt
     8341 cgtaagaagc tgcccagata ccctacggtt cccgcagtgc tgatgggccg tttggcggta
     8401 gatggaaaat atggcgggtt tggactgggt tctttacttt tggctgatgc actcaaacgt
     8461 atcagtaaag cagaaatcgc cgcgtatgcg ttgattgtgg aggcaaaaga cgaagctgcc
     8521 atcggctttt acaggcattt cggctttact tctttcgaag atgccaccaa gcatctattt
     8581 tttcctttgg cgaatcttcg ctaacccctt tttccgcacc atacggtagc ggtttctaac
     8641 cctgacgggg caatcccccg tcgccggggt tttgctccgt ctttttttag gagcatacga
     8701 tgtataaccc cgtatcaaaa atagacgaac tcctcccgga ttttgcagag ctggatcaaa
     8761 ctgtgaccga gatgcaggaa cgtcagaatc aaataactac tgatttcaac tttatcatcc
     8821 gtcaagtggc tgagactgcc caaaaaagta cggaagctgc cgtattgccg gaggaatctc
     8881 cggaaactga gccgccggat tccgacatac ccctgacaca gtttcttgcc gagtttgggg
     8941 atagtctctt taacgcagtt actgagcaaa accaaccggt ctatcaggga caaacttatc
     9001 ctctccttga agaagtgctg gataacctgg aacgcccttt gtttgcggcg cagcgcgaag
     9061 ttgtgcgtgc cgtattacag caattggtgg cagaggacaa acccgcagcc attattaatg
     9121 ccgaaatggg cacaggtaag accatgatgt cggttgcagc agccgctgcc gcgcatcaag
     9181 caggtttgca ccgcaccttg gtattgagtc ctccgcattt ggtgtacaag tggagacgag
     9241 agatcctcaa aacggtaccc aatgcccgcg tatggattct taacggagcc gatacattgg
     9301 caaaactgtt gcaaatccgt gcccttggcc gcaaacccga agttccggag ttttttgtga
     9361 tggggcgtgt tcggatgcgc atgggctacc attggaggcc ttcgtatgcc gttcgccgcc
     9421 gccggatacc ttctgaaggt gcttctgagg gttttgccgt ttccgagtat tactgtccta
     9481 aatgtggctg tgaaatcttg gatgaggaaa acaagcctta taacactgaa ggtggtgtga
     9541 aaaacgccct ggctaaaaac cgccgttttt gcaaaggcaa tcatcatggc aaaccgtgta
     9601 aggaaccgct ctggaccttg tgccgcaagg aacagagtga agacggggtt gtgagcacag
     9661 tgtacgatcg cgtacttaaa accatgacca ccctacccgg catcggtcct aaaaccgcag
     9721 caaaaatcat cgggatgtat ggtgagcagt atctggctga aatcttggaa aacaacattc
     9781 aggcgttttc caatttgatg gatgaaaacg gagaattttt attcgacgac cgtcaggcgg
     9841 cgcgtatcga ccgagactta ggcaaagcag aattcagcct gggacgaggc gggtatcagc
     9901 cgaccgagtt cgtcaaacgc tatttaccga agaacttttt cgggctgttg gtagtagatg
     9961 aaggtcacga gtacaagaac tacggtacgg ctcaaggtca agccatgggc gttttggcac
    10021 gttgctgtaa taagatcctg tgcttaaccg gtacgctgat gggtggttat gcggaagacc
    10081 tgttctttct attgtggcgg ctttggccgc aaatgatgat tgaagacggt ttcagttaca
    10141 acaaagggaa taccttgggt tcggcaagca tggcgtttat gcgtaagcat ggcgttttga
    10201 aggatattgt ccgacatctt ggaactgaat acagtaatgg cgcattcagt agctcaaaag
    10261 cagagcgtaa cagcgttcgc accgccaagg cgtcgggatt ttcccctttg ggcatcatgc
    10321 gttatgtttt gcctattacc gtatttctta agctgcgtga tttgggagaa ggcgttttac
    10381 ctggctataa agaagttttc cggccggttg aaatgactga agatcaacag gcggtctata
    10441 aaaatatgga gagtgttttg aggcagcatt tgaaggtggc attaaggaat cgtgacaata
    10501 cccttaccgg cgttgttatg agcgccttgc tgtcatggcc ggattgttgt tacaaacctc
    10561 aagatgttta ctggcgcaga caaaacaaga ttcttttcca taccgaaccc cagttcgatg
    10621 aagaaaaaat ctcaccgaaa gaagctgaca tgctggaagt cgttaaagac agtctggcta
    10681 aaggacgtaa atgtctggtc tatactgttt ataccgatac ccgtgatacg acaggccgtc
    10741 tgaaagagat tttcaaacgg cacggtatta aggcggcggt aatgaaagcg acggtcaaag
    10801 ccgatcaacg cgaagactgg gttgaagacc aattggcttc aggcgtacag gttgttatct
    10861 gtaatccgga gctggtcaaa accggtttgg acttgttggc atttccaacc atctactttt
    10921 tgcaaacagg atataacgtt tataccctga tgcaggctgc ccgccgttct tggcgtattg
    10981 gtcagaagga agacgtggaa gtgtatttcg ccggttacag cgatacagcg cagcaaattt
    11041 gccttgaact gatggggcaa aaagttgccg ttactcagtc aacctccggt gatatgcccg
    11101 attcgggtct ggacattctg aatcaggctg aaggttcagt tgaagttcag ttggccaaac
    11161 gcttcgttga ccgacaagat tgatgcaatt tttgcatcta acccgcgttt agtgggtttt
    11221 ttcagacgac ctcgtgttga ggtcttctga aaaagcccgt aaggtggaat ttcacaggct
    11281 ggcagacctt atctgcccgc gcactgccgt tacccattgc gcacgtcctt cgggacggac
    11341 ggctcttttt caactttctt taaccctgtg gggatatacc gtatccccac agggcatcgg
    11401 tatatctccc tttttcatgg agataccgat gtgaaatttt ttatattcat caaacccgac
    11461 cttccttttc acaaggaaag cattgaatct tggatcaaat tgctggaaga tataggtaaa
    11521 acagccttgt tggcaatgct gccgttgttt tggctcaatt catactcttt gaccgaacgt
    11581 gcttccggcg tagccgtatt ggctttggtt gcctactttg tgcaagtata tgctgacaaa
    11641 ctacggaaat accgtgattc attaaacaga aaggatacgt catgctgata ggcggtctgc
    11701 tatttattct cttcctggtg cttgctttcg gctattggat taccaagcag cttgaaactt
    11761 cgcgtacgtc ggaagaacac aaaaaccgtt aacgcggttc attctatgta gctcaagtat
    11821 ttatctttta cttggataaa tatgaatcaa agattgtcaa acaggaaccc aaggaaaggg
    11881 aaaaaccatg atcatgacct acagcttact atttttgatt gccgtcatgt tgtttggcat
    11941 atttaaactg gtaaagtttg gcgagtctca gcaagacact aatacacatg accggtcaag
    12001 ttaattggta ttcccatctt ttaaatgctt tgttgctatt taataccaaa gcattttaaa
    12061 tagaacgctg atacgttcct ttttagataa atccggcttg aagccgcaat cccgctgttc
    12121 cgtacgtctg tacggcagtg ggatttttct tggaagtcat gcggttacgt tttttttaat
    12181 cgtatgcgtg tatttttaac catttgcagg aggaaaacct atttttgtat cggagaagaa
    12241 atgagatata gcaaaaacaa agattatcaa ttttttatac gccaactggt atcgggggga
    12301 gaatggatgt tcctccccaa aaacggtcgt aagcacagcg cattgaagca cctaccgacc
    12361 gatcgaaaaa ttccgatacc cggctcgccg ggacaggatc cgcgtggatt gctgaatttt
    12421 aaaacaatgg tacgtcatat agaacgtggc agtaccttcg attaaacttt gatttaaccc
    12481 tgcggggact gtcccgcacg gcatgtctcc gttttttatt tgaatggaga ccaatatgga
    12541 aactcaaaac catctgatgc acccccgtgt cgcgcataac caccgtgaca acggatactt
    12601 ccccaccgac caggccacat tgtcgcgtat agccgcttgc cttgatattc cggcatctgc
    12661 cggaggtttg ttacgcatac ttgatccttg ctgcggaaca ggagaagccc tggcttcttt
    12721 agcttctact ttgcaatcgg aaaaaataag ttgtcaaact tacgggattg aactggagaa
    12781 agaccgtgct caagaggcaa tgagccgact gaccagtgtc atcaaatccg atgtagaaaa
    12841 ctgcaccatg cagggttcgg cagtcggcct cctgtttctg aatcctcctt acggctttgc
    12901 cgccaaagac aatctgtcta atcaaagaac caaacggctt gaagaacttt tcttcgcacg
    12961 taccttcggt actttgcagc ccggcggagt gttggtaatg attgttccgg aagccgccct
    13021 gacagagcat ctaaccggcg aaatcgctac ccactgtacg gatgtccgta tgataagggc
    13081 tgcggcggat acctacaaac agttggttat attcggcatc cgaccgaaga acaaagcctc
    13141 tatcagcaaa aaacaggcgg atgcccaaca aaggctcctg cttgattttg aaaatgcccc
    13201 tgagactttg ccggccggta tgcctgcata ctgtgtgcct attgcaccca ataaggtctt
    13261 tcgccccaga agctttaaat tggagcagga tatattggaa cgtgaactgg gacagcagca
    13321 atcccgcacg ttatggccat cattttcgca atatttcggc accagtgccg ttatagccga
    13381 aaaacgccgg cctttatgtg ctctgggtca gtggcatgct gctttagcat tagccgcagg
    13441 gcaagttaat ggcgttgtca cctcaaccaa tggccgaaag ctgctggtta aaggttcaac
    13501 ccataaaacc aaagtggaaa ccaaatcgga agagtacggc aagaaggaca ggcttgtggt
    13561 cacgattact tcaactgacc ggttttctcc aagcatacgc gcaattgatt tgaccgaagg
    13621 atcgtcggat tttggacgag tcctgacaat caaatagatt ggcctattca atttttaaac
    13681 cctgccgggt acttcccgca ggggtgtatc cggttttttt aaggatacgc ctaatggaac
    13741 aacttgattc ggcagctccg gccgcatgtg ataccccctc tctgtttgac ctgggccgag
    13801 tgtctatgac ggcaacagta gattaccggg caaaagagca cctgcctgca caactgtatg
    13861 agaacagtct ttatgcccaa atattgcttg aggcccaccg ccatggcata tggggtgata
    13921 ttcctcctga ggatgccaag ccgaatcagc tggcactcaa gccaggtgaa gaaggcagaa
    13981 taatgtcttc ttacaagatt ggggaccaaa aaatttgggt tgttacagaa tgggacagaa
    14041 gccttaccac actcctgttc ccagaagatt actgaccttg taatcttttt atatatctca
    14101 cctgccttcg ggcaggtatt ttcaggcggc tttagtaagg ccgtctgaaa atacctgtct
    14161 ttacaggctg acagacctta tctgtcttgt gtcctgccgt taaacatgac acgcatcctt
    14221 atggatgtac ggcattttct tttatcaacc ccgtggggaa cacaatccct acggactgtg
    14281 ttctccgttt tctttttaag gagcaacaca atgcaaaact ttattgtatg gatgaatccc
    14341 gatcgggaag aatcggcagc tgcggcagct aaattgaacg gtgcagtttt gaccgctcac
    14401 acggatggtg tcatggccgc tattagacgt gaactgaaaa gatttggatt aggggctgat
    14461 ttggccgtct ttgcccaaac ctgtgaagag tttgccaaac aggctaatgc cgacatggtt
    14521 tgcatcagca gtagcaatgg tgatgccgtt gcagccgtca cttaccttga ggacggatac
    14581 gccggtgaag gcgtatccgc cgttgccgac atgcgctatc tgatgcctgc ctatcggcag
    14641 tatttcaacc gtcctgacaa aatggttatt tttcaggggg gcattgcagt atttatcaga
    14701 tgaagacatt gcctggattt tccgccgcca catcaatgcc atcatcgagt ggcagtcgaa
    14761 tgaagggctg gggctgtacg agttcggttt cagtctatct gatgttctga ctgatggctg
    14821 ccgccatgtg ctgcgcgaag tcgccaccgg ctggctgaac cgcgaaatcc tgcccgagct
    14881 gtacaatgtg taggcataac cgccgtgctt cttttccaac taggtaaatg cccattacct
    14941 gcggtctttt ggccgcaggg gcagaaactc tactggagat agttatgtct atcaaagttg
    15001 acaacatcct tctaatagaa ggaaccaaag aatcggttgc cgaagcggcg aaactgattt
    15061 tatttcgctc ccccgttcat cctgagcatt gggcttttga tttcaatcgg attttgcctt
    15121 tacccgaaaa tatcacgtct gaccttgcca aacagagaat cgaggcatgg ggcagcagtt
    15181 tttccccata tcccaccgaa acctttgtta cacaaagaga gggttttatt gaaatctgct
    15241 ttttaaccgg attggcaccg cctttcggta tttatcgcaa actcgcggaa atcttcgcca
    15301 acttggatgt atgttttaca gcgaaataca tcaaccagta cggaagtttc ggcggacgct
    15361 acgaataccg taacagcgtg ctttaccact gtctttgcga gatggccgac atccgtaaat
    15421 tcggtatcgc cgaattcgat atttggtacg aaggcttgtc cgatagactt gaaagcgacg
    15481 ggtatttggt agaagattag actatgttac acggtaggca gtccgtcaaa ccgcccgcat
    15541 accgccgtta accaaatgca catccgtttc ggatgcacgg ctcttttatc aaccctgaag
    15601 gggatgtatt ccccgtcagg cgtacatctc ctttttttat ggaggtgtat atgcctgaca
    15661 atatgaaatc gaaaacaaaa gtacccactg cggtactggc ttcatgggcc gccatactgg
    15721 acgatattgc caaagcggcg attatcggta tcggtgtggt ggtctggagt aacggatata
    15781 gtgaggagat gaagaatctt gccatggcag gattggtgct cctgtcgttt gaaatgctgt
    15841 atgtttcatt ccttatccgc attcacatct ctaaactgga ggacaaataa tggacttcgt
    15901 taccggaact tgggtcatgg ccgcagtcgg cctggttctt gttattgttg cgagaatcta
    15961 tcttaaaacc cccaaaaaat aaccgtgctt aagcacattt ttcatcaagc cggcggaacc
    16021 gtcgttccgc ttcccaaagg caaagttacc gtttcggttt ctttgccttt tatctaaagc
    16081 acctgtcgtc tgaaatgtgc gccggtttcc ggtggcggct tttttatcaa cccgggcgca
    16141 agcccatcaa cctgcgggtt tccttatccg cagggatggg gattcctgct caggagacta
    16201 ctatgcccaa ctggtgcggc aacagccttc attttcaagg caatcctcaa cgtatcgccg
    16261 aagtggcgga gcaaattctg atcaaaaccg acaaggggta tcaattcagc tttcagcata
    16321 ttgtacccat gccggaggag ctgattcttc ctgaagtgcc tgatagttac atgcagcaga
    16381 cctggtttca aattgctcaa caacctgaaa agaagttgat caatttggaa cgggaaatgc
    16441 tggaacagcg cgataaatcc ttttggtccg ttcaatatag attgccggat gaacttttct
    16501 ctgaattaaa gctatatttt tcggaaaaat ttgattggga caatctgact gttggtgaag
    16561 tatttctcca ttttgaagct aactctgaag tcgcatctcg tttccgttac gatgaaccat
    16621 ttgccaagcg tattgcggaa aacatccgcc aatacggtca tccgaattgg tatgactggc
    16681 ggttagctaa ctggggctgc aaatgggacg taaaccccga ctgcactttt gtgacagttg
    16741 gggaaagcgg catccggatc agctgtgaca ctgcctgggg tcctccggaa gggatttata
    16801 gagaactcgc caaacgtttt cctgacgtag aatttgaagc caaatacctg gaagaaggaa
    16861 tgtggtttgc aggaacctat gaaggtcatg aaggagcatt gttcgattat ccgtgcacag
    16921 atgatggagt ccgtgacttc gctaccgagc actttggatg cgaatacgat gacgaagatt
    16981 aaagaactta gatttcgtta gacgtattgc caactcaccc tgcggggaca acccgcaggg
    17041 ttgtcccccg cttttttgtg gagattagaa atgattgaaa atcagattga caaagagatc
    17101 actcaggctt catgcgaagg ccgattcatc ctaaaacagg aaaacggcaa acgtttcctt
    17161 taccttaacc tgcccgaagg tagtgacgaa ttaaatacca tttggcagac ggacgagtat
    17221 gactttaccg tacccgactt agaagtcagc atcgatgttg aatcccttta cactgctgtt
    17281 agacttttga acgaaaacca gggcattttg cacagtatca gcacaaaatg cagtgcgtat
    17341 tcgttcggtt tcgaaggaaa gttgagatac gaaagattgg atgtaaagcc tttccctatc
    17401 aaatcgttct cttattattt ggagttttat aacgactgga cggggacgtt gtatgaactg
    17461 gacttatcag cgttcctgga tgagtttttc ggagagtgtg atcctgaatc caggctcgat
    17521 gcctgtttga agtagcattt gtgcctcaaa taatcttttt caaaaccaac ggccgctaat
    17581 gcggccgttt ttttgtgccc atactctgat atttttgggg tctctgcccc caatcccccg
    17641 gtctcgccgc cgacagctcg ggaacaggaa ttacctccgg cctacgcctg ctccctcact
    17701 taagcgtgtc atcgctgcca gtcagtaaat gggtattcat aaacgccctt gcaggtcgaa
    17761 ggggttgggt attgaccctg taaggaaatt tccttacaat aaacagaatt ctttaattta
    17821 agcagttttt aacaatatca ataatttccc aagccgttaa atagaagatg tgcgtaacta
    17881 ggacattgaa gtcgcaagta gcaacagtaa atttgaatga aggcagggca aagagaccat
    17941 gaagattatt tttgatgcag tgtcaactat gaccagcaaa aaccaaacaa ccattcccga
    18001 gtcagtacgc aaggcgttgg ggctggaaaa acaggacaaa atccgttttt ccgtcttagg
    18061 cggcggacag gtattgctag aaaaaaacaa tactgaggaa gaagaatttg agcatgatcc
    18121 agttgttgga aaattcctgc attttttgga aaattctatg acaaaagatc cgaattccat
    18181 caaacctaca tctcaatcac gctttaaccg ccttcgccaa ctgacttggg aaacagatat
    18241 gaaagactaa tatgcctgat tcccctatgc agataaacgg atataccatt tacgaacacg
    18301 aatgctttac gcagcaatta gacagactgg ccactacgat agaaagccag aaacgatccg
    18361 aaaaatccag tagatccgaa gaggccaaac tgctggaacg aatcttaggc gaaatagaaa
    18421 atattgccct taatccggat agcccggaat atcggcttgg caagacactc ggcccggaat
    18481 acaaattttg gcgacgtgcc aagtttatgg gtagataccg attgtttttc cgttacgact
    18541 caaacagtaa aatcatcatt ctcgcatggg taaacgactc gggtacgaaa agaacttatg
    18601 gcggcaaaaa tgatgcatat accgtgttca aaaaaatgct ggggaacgga aatcctcctt
    18661 cagattggac tgcactgctt gaagtagtat caaaggaata gagcaaaaga aaacagccct
    18721 tagaagattc tcatggtcaa aatacagtat tagactgaat gttacaacat tgtaataata
    18781 attgctgtta tctataatgt tgcccatgtc cgtatatgag ccggacaaat ctaaaagggg
    18841 gaagaaaaat tgccctttgg cggcaaacag gatatatagg attttattgt tgtcttattc
    18901 tgatgatgct tttcttccca aaggatttta atcgtttgaa aattattgct tatacgagtc
    18961 cggatggtga tgaatgggta actgcgctgg aagcaaagct aaaacaccgg gaaggaatga
    19021 tctactacag ccgtcatcgt cccggtacaa gaaagaaaat ggttttgacc aggaggaagg
    19081 cgaccaattt ttttcgctat tacagtgaag ccgactccgg tggtgcttcc gcccctgaat
    19141 cgctaaccca cttgctttgc aaacaagtgc tgaacgaact atccaactta cccggcggcc
    19201 tgacgactgt tctaaattgc acagaacacg ccgagcaaca accgcctgtc actattaggc
    19261 tgaaccgtgc attatccgaa tatcggatag atatagatgg caaaactttt tatattgatg
    19321 ttttgcttga atttgatcag ccgggtaaca cttccctgct caggcacgag ataaggtggc
    19381 agagaaaatt ggcggttgag atttggcata catcacggtt ggcttccaat gcgcctaaat
    19441 gtttagcctt atcgaaaata ggtatcccgg tggttcagat acgggcagat aagggttcat
    19501 ttttgtatat tgatgaagac gagctgctca attatgataa tgaagaaatc aaaaaccgca
    19561 taaatcgcca tgtagagaaa cttcggaaca cctttcggaa acaaattctc tgcaccctac
    19621 tccgtaatcc tttgagtgcg gattttcaga cggccttgat gttgcataat caaattaagg
    19681 ccgatgaaca acaagcagag caaatacagg aaaaatttga ggcgctgagg aataaacatg
    19741 ttttattgga agctgaatat tcggctttgg ccgcccaata cgcagcctta ttagaacatc
    19801 agaatttcca agctcactcc gggaaaaggg aaattccgaa aaagcatggg attctgcagc
    19861 gcatggtcag ttggtttaaa tcataaacca ctgtttagaa caaaccctaa gtttcaataa
    19921 gatgaggaaa atattgcaat gaaaaaaatt atgttcggat tattagcagc tatggccatt
    19981 tcaaatgccc aagctgaagg taatttcttg aatcttgctt caaagccggc caaaattgac
    20041 ggggtattgg aagcttcttt agacaaaccc tatcccaagt ttgtacaaga ttggatgaaa
    20101 gacagtaaat tggcgggact tggccgtatc atccaaggca agatttttga aagtacggga
    20161 actattgaac gaaaattcta tgcaagatcc gaatccgaac atgaaaaaac ggttgaggat
    20221 gcagtaggaa ctttcaaagc atattgcgaa catcatcagg ggacggttga aaccctgact
    20281 tccgatattt tctgtatcaa cggcagacaa gtccagtgga tgggatggtg gtacagagaa
    20341 aactatacga attttgcatt ctctgatgga tatacagggg agcaagtcta ccgcagcaaa
    20401 gttacggatg tacaacccgg taaaaaaatc tccacttcct atgggtctgc cttggtctta
    20461 gaaaaaggtg agaatggctt aattaaaatt cagccgaaca gaggcagcga acgttggatt
    20521 acaagtgaca gcatccaagt aacttataaa taatgggatg tgtatcatat aagaaagagg
    20581 attggtagct gaaatgctgc caatcacatg gagtgcaaat tgaaaacaaa atctaaaaaa
    20641 cttttgaggc ttgttatctt atttgtagcg gcattctcca tctctacacc tgcggtaatg
    20701 tacttcagca caggcgaagc gacattaaat ctttttgttg aggggttttg gataattgga
    20761 ttacttcaag cactcgcttt taccaccatt gtagactatt gcactgaatc taaacataca
    20821 aatatgctca ataatccagc gtgcaacgat gaagattggg ataatgatat gctcaataat
    20881 ccagcgtgca acgatgaaga ttgggataat gatatgctca ataatccagc gtactcttgg
    20941 tcaatgttca atattcacca ttcagattaa aaaagcagga gccttatact cctgctttcc
    21001 gcagcaaagt tacggatgta caacccggta aaaaaatctc cacttcctat gggcctgcct
    21061 tggtcttaga aaaaggtgag aatggcttaa ttaaaattca gccaagcaga ggcaatgaac
    21121 gttggattac aagtgatagc attcaagtaa cctataaata aaggagaagt atcatgatcg
    21181 gattcgtaaa atggtatagc tatgagaaag attttggctt tatctttggt gaagatggtc
    21241 atgaatactt tttccgtaaa tccgccggtt cagatatacc aggacagata gtgaatttcg
    21301 aaccatttga gcatagtcgt gggctgcgag cgcgaaaggt tggtaatata cataatcctg
    21361 atcctcaaca gatccaaaaa ttgaaaacct tatgcaaaga ctttcagaaa gttgaaaagc
    21421 aacccatgtc taaaaaatac aacttgcttt cggcagctat attatcgctg tgtttgacta
    21481 tgttcataac actgggcttt ttctacatct tagccggctt ttttgatact gatagggaga
    21541 ctaacatagt tttgctgttt gtcttccctt cggtaacttt ttccgtgctg acattctttc
    21601 tgaataagtt attcaggaat atgaaactgg ggtctgatgc tgatgttgtc accaataatg
    21661 attggttaaa cgatttctgg gaggataggc gtcggcactg tccttcctat tcacatcatc
    21721 cgagcaatat ttggcattta tctgaggatt gattaattta taacccagct ttttccaatc
    21781 tcaattgcaa tatatttcat cgatcaaatg acgattaata aaaaaaacag gcagttaaaa
    21841 gaactgcctg tttttttaat cgtctcctag aaccccctca attgcttcaa gggttttata
    21901 aaacacagtc tttgcctcgt ctgactgcaa ttctgtaccg ccactgaaac ttaaccgtac
    21961 attgcagtca tccaaagtcg aagattcttc aatttcaatg acaatatggg caatgctcat
    22021 gatatgtacc cctctctatt tgattttacc gatttcattt aaagtttttc cgcgataggc
    22081 actcacaggt aaaattttac ccgatggaga gtacagcatg gcatcaagat gcggacctgt
    22141 tactccgcca ctggctccaa gatttccgat ttgctgccct ttacttactg tttcaccaaa
    22201 acgcagcggg ctggcaccat tcatgtgaga agcccgatac cttacgccat ttggcgcttc
    22261 aattacgaca gtgtgtcccc agccgacggt tttaccgttg actgtattcg cttgatagcg
    22321 actttccact actctaccgc taaacggtgc cagcaatggc gtattgatag agccacgata
    22381 gtcttcgcct ttatgattta tgactttctt caaaataggg tgaacccgtt ttccaaatgg
    22441 gctgatgata gtatgtgcgt ccggtaatag tggacccaaa gtttcatttc ctgagccata
    22501 tgactttgcc ccattgccgc gatagccgat accgggcggg gctacttctt tgggcgatag
    22561 ttacgagaac cacttcccgg tcgcccttca gtcttgccat atttattttg cgcctggaca
    22621 tatgctttat cggaagagaa agtttgtgta gcacccataa caaaagtgtt tacatgtttg
    22681 aaggtgtctt ctccttgagc atgcagtttg tttgtatttt cttcttgttt tttcatacct
    22741 ttgtcaaaaa gcttctgatt aaagttcttc gcatctttgc ttccctgttc tagtttttgt
    22801 tgccaaccgt caatatttgc tcgggcttca tttctattaa cgccatttcc gtcagcttga
    22861 gcctggatag tggcaaaagt gctagatttt tggccgtcta tcccagactt ataactacta
    22921 taagcagcag ccacattatt agtggggcga tcatcgggac taatcggtcc tgtactgtca
    22981 aaacgggtac cttcagcttg ctgtcttaaa tttgaagctt gagtaactgc tgcattcatt
    23041 gccgcagctg ctacaataga cggattattg tacacttggc tgtaggctag accgttctga
    23101 tcagcatatt ctcgaattgc ttttgcgtta tcaatactaa cagtcgcacc ggattgtgcc
    23161 gtacgggaag cagcttcgcg atagttttga gcctcgttat agctttgttg tgcgctgata
    23221 ctggcctgat gtgctttgtt atagctggct tgagcttgag aagcaaggct gctactgtca
    23281 gttagagctt gagtaaacgc attactttga ccaatatttt caagcatgct tcttgtaatg
    23341 ctgacaccct gggatttaag ggctttggct gtttcgctaa tatcatgtga tagtttgttt
    23401 gaagatttac cttcacccct aatagatgct cctccactta ctgtagcact agcctgtggc
    23461 ccggctccct tagatccagc tgtcacacca cctgccaatt gggctcccaa atcaagcgca
    23521 atagagctga ctgtctgtac atcgttgccc ataccgtttc tttgagcaat atcttttgaa
    23581 gattgcaaaa tttgatccaa agctttagcg gtagaactac tcatgccatt ggtaactgca
    23641 taattcgcac cgttttgacc gacagtttgt atgccgaaag ccaaagtttg cgtattaccc
    23701 aatgtagttt gttcaactga agacgcagca tactgtttgg ctgccgtcgt gcttcggtcg
    23761 gctgcttgag tgtaaacatc agacatactg acagtagagg tagatgagac attgccaaag
    23821 ctgtcccccc ttccctgata gcgattttca ccagttgcca tattccgcca tgatgttccg
    23881 gctgcagatt gagcagtcat catttgagga tcggtatata cagggtttaa gctatgtttg
    23941 ttggcattgc tattgttgta gttcatattg ccggcagagt aattccccga ccctacttgg
    24001 gaacccgctg cttcaccggt tgatttggcc gatgaagtca tgcccatcat gccgttcatc
    24061 aggctgcgtc cgcccatggc aagagcaccc gcgatgaccg gaatcagcca cattgcagcc
    24121 ccagcagctg cttgtgcatc ggctgctgcc atcaataacg tatcacctgt tcccggtgta
    24181 ataacgccgt tggcggcttc cacgctaaac agacgcttcg cattcatagt cataatcata
    24241 ttgatgatac tggctacggg tgcccaaagc tgaatccata gcatactcat catgtagaag
    24301 cttaataacc ggaatgccat gatgccaccc aacatggcga gaatcattac aaaggggaat
    24361 aaaccaatca gaatggcctc agcagcagaa cgaatcaagg gaagattcca acttgccatt
    24421 gatgagccgg ttttttgggc tgcaatatac tgattacgag cttgaacttg agcaaatgcc
    24481 tgctgtacgg cagccggatt tcggttgcgt tggccattga taatgcctgc ttgacgaata
    24541 acctcaacca tcgccgcctg ttcgatattt ttcaatgtat cgaagcggga attgtccagc
    24601 agatattgga atgagctatt gcctaaggta accagggcat ttacccggtt tattcctgcc
    24661 gaatttgtac ccaaacctgc aaaaacacga tcggcaatgt agccgggcaa gtaatttttg
    24721 ctataaaacc gggtacggtc ttttagttct tttgccgcct cattgcaggt catggtcttt
    24781 gatttgccgt tatactgcaa catgttgcct cttcttgagg cgatctgcga tacattggta
    24841 aacaaggctt ggttggtatg ggtcaatgta gccagaatat cggcactctg tgccagatcc
    24901 tgcacgttat agaagtgata caggttcata tcaaagaatg aacactgatg catgaacagt
    24961 gcccagtctt gagtcagttg cgcgtccggg attcgtgctt cacggatatt ttccatcagg
    25021 cgcgttccga acagcatgcc ggtgcgtgag taattgaggt cgtccggcag gtggttaacc
    25081 tgctcggctt tgcgggtaat actttggcca aaagaactga caaagtagcc catataagca
    25141 aggcctacgg gtacattacc gacagtatat tgacggcctg tatatccata cccacctgat
    25201 tcagtaatga gtacagtggt tttagggacc atcataccca tccaacaaaa taacacgaca
    25261 aacaaataac tgaagttatc cttcaaatcc aatttgaaga ctgcaacagc aagagtgacc
    25321 aggaatccaa ataaggcagc agcctgcatt gcgccaccca tcatcgtaga tcctgatatt
    25381 gtggcaacgc cattgaaaac cgcccgtaca gcttctccgt taccagggaa agtaaagtat
    25441 tcgacagcca tcatctctcc ttagaaacgg ttcatcgaat caaaagtcat ggagttgttg
    25501 gtatccacaa aggctgagcg ccattgtgtt tcaaactgct ccatagccct catcatgctt
    25561 tcttcgatct cgattttctt cattgagtca atgcgctcct ggtaaattct gcttcgcaat
    25621 tcgccaaggt tggcaaacaa ttttcgaacc gcttccccgc tggcatcgtc tgtcttgtta
    25681 tacataccca aggtcgagcg tacctggcgt tccgattggt ccaaaatatt ggccataata
    25741 tcaacggcaa tcgcatctaa aaccgctgtt tttttcaaat agccagttcc aaggaaagca
    25801 tctgatacag ccattttcag tatgggcaat ttactgttat tgacaatggc agcaatctgc
    25861 ttgcgcgtag ataaattcca accttggtta ttccttattg cagtcggaag cgtttcaatg
    25921 cgtttgtgaa ccatgtaccg caaagaaggt atttctacat catttttctc agtgcagttc
    25981 tccattaatt tcttagaacc gtctttgcac gtccacatat ccaatacaac cttctcccgg
    26041 atttctcccc cggcattgtt ttttccgttt aaaagatctt ggatgctgag gatttttggt
    26101 tcgaaacttt gaatacctac tgtgtatttg tccttatccc cttcatgttg tgttaatgga
    26161 gtgaacacaa ttgttcccag catgctgtaa tagaagtcca tgtcgatacc cagtttcgag
    26221 cgttgacccg tatagttctt catggcttca taagtcaaat taccgccggt aaagctaatc
    26281 gtatcttggg gttttccggt gtttcgcacc ttatcggctt catgaacaag gtttttagcg
    26341 gcctgacagt accaagtacc ttctgccggg tcatttgacg aacccgaatc aactgatgcg
    26401 gcgcgacaat ttgaactggc caacgaaccc agtttcccca ttgcgccgcc gacaatatcc
    26461 tgactcagtt tgcaactgtt taaactgttt tgattaacat aattgaccaa atcacgaata
    26521 ccgccaattg catcttggat catcggatca agggccttaa gtgccaggtc aaaggctaca
    26581 cctgctgcgt tattgcccag gtttcgcgta aattggatga attgttcttt attgacaaac
    26641 gagaaagagc cggcaaagaa atctataccg ccgcaaccgg cactgattga aggtaattga
    26701 atattcccaa ggctgatatc cgacaccgga aagcgaacac tcataccgcc tcccgtatat
    26761 aggctgccgc tttgaccgtg gaacgcacct gcttcagtat aattggcttc cgctcccatt
    26821 gagttaaaca tgtcagccat gttcttctct atgccggcac tactattgat cggaagggcc
    26881 agcgatgccg ccaagcatag atattttgca gaggaagcac aaactaccgg cactttggcc
    26941 ggcttcggaa atcgaaagat gtgaaatttc ataaccgctc catgaagcgg ttatagcagg
    27001 gaaatcctta ctataaccaa gataagttac agtaaagact tgagattaag ggaatcttat
    27061 tttacctgaa atatccgcgt tgtcaaccgg ttaatacata gaaatataaa tattgaaggc
    27121 cccgtatagg gcctttgatt tgattaatac aatgtcccga cagggcggga atacgctaag
    27181 aaacgatcct caagcgtggt ttgcgccatc accccaaatc caatgggtgt aaattgcttg
    27241 gtttttgtat ccatgacgta cattgcaggt acagtagtaa tattcagcct ttgggcaata
    27301 ccgttgttcg gcaaagcacg cgggaagtga ttaatacctc cgccatcgag actgaccggc
    27361 ataaccttaa taccgtattg gtcggcaaag tttttcaaaa tcggcgccat ggtatgacag
    27421 taagggcaat ccgatttgaa aacaaacata atgccgttct ctccggcaag ttttctaatt
    27481 gccccggcct tttgttgctg acggttatga tcatattgtt gctgggccag ttggttgttc
    27541 ggacgaccgc gctggctgta atccaaattt gggttttgcc aaattaccct ttgccattga
    27601 tcggcaaatg ccgcagattt gttcatcaca acctcttgaa gttcaatata tcgcttcaaa
    27661 ttttgaggtg tggggttcat gacggcaatt ttacgcgact catccaaatt tctttgcagc
    27721 atttcaaatt ctgcaagttc attacgaacc ggagtatttt gaggccggtt ttgtccggct
    27781 tgtttgggct ttttagcatc cggataccat aagaaagcac ggttttcgtt tttccagata
    27841 tcttggtcat accatcccat gccatcatcc gcagctcgca gattggaggc gtgcaggcta
    27901 aatatcagca tgacggccat ccgcactctt ttcatttttt accaccgttc tttctgttcg
    27961 ggcacgcccc gatatccata atttcagacg gaaaggtttg cgacagtgcg ggcgaagtat
    28021 ggaatgtcaa gcgaaccctg ttgcatccgg gtttgttttt aatttcttcc attctgaccg
    28081 cctcgacaaa aataggtatg tcctgccgga atttctgttt gacggcagcc gcagcacggc
    28141 cgctgaaata cccttttgcc gaaccgtgac gcattgccag cgcaagcaag gcatccggct
    28201 tgccgtcagg aagcgggatg gaagtccttg ctagagcttc tgttttttcc aaagcgatac
    28261 cgccggtata aggaagggat gcggcaagtg ccatacctgt aaaaaacata cccgatacaa
    28321 tacccgccga cgcagccaac caattattga tattcataat agttcttcac ttccaaattt
    28381 ttcccattaa cggatgcctc cctgattttt tgtgaaacct tttgcccctg cttggccacg
    28441 ttgtccatgt tggcatagat ttcttcataa aacccggaca gatcaagttt ggaaaagtct
    28501 aactgttcca attcctgtgc ggtaaatcct tcgcatttcg ggttctccgg cgtaccccaa
    28561 ccccgtttca attgctcttt gccgccgaca ttgactattt tggctaattt ggaaatgaaa
    28621 cagcactggc tctctatcat tgttacacat gcccccaata ttttgctgcc gcaataagaa
    28681 ccgacataat ggcacaaatc tgctttacgc ttcatggcaa ctaaaatttc actttgcggg
    28741 cacttcaacc actgttggat ggcgattacg gcaatagcag ccgcaaatac ggccgagttg
    28801 aagccgacaa ttacccccat accctgttgt cccgcagcag agaaacccaa cgcaccaatc
    28861 tgagatgttc cggtaatacc gccggccgca ctcagggccg tacccattgc ctgtgctgcg
    28921 gagctgacaa acatagtggt aaacgtataa tggctggcaa cgctggccat aaaatacgtt
    28981 gttgccatag ttgtccctgt ctgtaccgcg aagtcaacaa atttgcccgg atctgctttt
    29041 gttcgacagc agtctccgcc gaatacgctg tctaaagcac catccagctt gatagagcat
    29101 tgcccggctt ctccattgaa aaaacgctgt ttttcgggat cataaaaccg ttgcgtctca
    29161 cgggcgaatt ccatcgtcgt catagttttg gcaaaatccc gatcctgaca accggatacc
    29221 tctttggttg tctctccttt ttcggtacat tcaaactgtt tttctctggc ttgacaggta
    29281 ccgtccggca gctgatgcgt acacttgtcc ccaatcagac ggcattggtc tgaggatgta
    29341 taagcgctgc atgtttcgga atcgcttccg tccgtacatt gcacggtgcg cctttctttc
    29401 cagcaatcac gtgtaacggg tacgccgttg atggtcttgg tactgccggg ttccaaacac
    29461 tctgttgcaa ctgctttaca tttttttcct tgtacctgac atgacgactc atcccattgc
    29521 gaactaatca gatgggtatg ctccatttct tcgactttgg tcggaagcct tccttgatgt
    29581 aacgttctca aatccacatc gcaagaatac ttgtgcgtgt aacgcaaaca ctccccctca
    29641 ttcgactgct ccaaacaggt ttgtccctgt acttcacatt ttgccgaagg gtcttttaac
    29701 ggtgcgcaat aatcggcaga atccttctca aggcactgat actcttcttt gtattcccaa
    29761 cagtctttag ttacgtcgac cccgttgatg tttttggtcg acgggccatc aacacaaacg
    29821 gaagacgtcc gggtacacgc tgcactttcc ctgagtgcgg cagcagacgc tgaagtgatt
    29881 aacataaagt ggcagatcat aaccagcaaa gacgttatag ccctcattgt tttaccctct
    29941 tctcatactc cgaacaactg ttttcccatt tatcgtgaca aacgggctcg cagtattggt
    30001 gtacattaaa gaagatagca ttttctccgc cgcctccgac aacggtgcgg gtccaaatgg
    30061 tatttttgcc ctgatgcaga tacggccgta tgtcgatgtt caggtttttc ctccaggatt
    30121 tacctctttc agcgttaccc aaccgagtac cttcctcatt acgtaccggt gcaaaccttc
    30181 ttcctatagg aaatccatct tcatcttttg cagtataatc tgccgcaaac atctgattgt
    30241 tatagctgct gtacacaatt ttgccgttga ctttgacaat cagccaatca tcgtactcaa
    30301 cccggctcaa agtaaacagc gtcagttttt ccggattttt cacattgagc gtcatctccc
    30361 gatcatactg cccgtcacgc caatagttgt cgccaatagt accgaacatc agcctgtgtg
    30421 cgcttcccga agccgtcagc ctgaccgtca tatcaccctg caggctgcct gcttctatac
    30481 ccgaacgggt acagtttggt cctgatttct cgcataccac ctttaatttc ttgccgcatt
    30541 gatactgctg gtttcgatat ttgctctcca ggcaggaata gttccagccg ggatcgacat
    30601 tgaccttaag ggccgcctcg cacaagaaca tctctgcgcc atggtattca ttgcaacgtt
    30661 tgacggtttt taccgcagga acggtaatgg tttccgtagg acatgccgac gagggaactg
    30721 tgccgacgct gccgtcttca ttgacgggat atttccattt gtatttttcc agtgtttctg
    30781 ccggatcgcc tgcaattttc tgcgtgcctt taaccaactt ttcatttgaa gatatggtta
    30841 catcgggccg cttggtacgg cttcctgcaa tcaggtttac accttcgcat tcttgacgaa
    30901 gatagagatc cggattggca ggcagatttt tacagtcgtt gaatttggca tcaccaaact
    30961 gagtcggaag agaaactccg ccaccgtaat actgttcagc cattggtacg ttgtctgtat
    31021 atcttggggt atattgggca ggctgcatca tgtttgttgc agtcccggcc cccaattttc
    31081 ccagttcgcc gacgtaacta ccgtaatctg cagcaaaggc cgtctgcatg aggccaaata
    31141 cagttagata aatggcatgt cttttcattt tctgccttcc agaacggcta atttcgcatt
    31201 tgcaatatcg gaaagttttt tatcttcaga ctctcgaatc aagtgttcca aagccgaatg
    31261 caaggacaca tcacccttta cggcaacatg ttccgtacac atccccaatt ctgaatttac
    31321 agcgcaaccg tctaaaccat cttcgctgtt tcgggcgatt accgtaacag ggacttcggt
    31381 gatgccgtaa tggtcgaaga gttcgggatg aatcagcata tcgccttgca aagccgcaag
    31441 ttcttcggcc gccttaaccg tggctttcag tgagtcgtct ttaaaaccgc ggataaccat
    31501 tacgccccct gcctttgccg tttcacgggc aatgcgtttc aagctggctt cgggcattga
    31561 aaatgatacg aataccatta aatccgcttt ttccgcagcc tgtcgggctt cttgtgaagc
    31621 agtgcggtat ttttgtgcga tagacatcgg atcagccttg atttttgcat ttccgtaatc
    31681 acgaacatcg ggcacacggt gcttgatacc tttcaaatcc ggaatttcag gttgctgccg
    31741 attgtcaccc acatttgggt ttttaactcg ggtaacttta cccatggttg tacgcagacg
    31801 gtttcgcgcc gcctctacat ccgcatcggc gtaagcactg cctactccgg ccaaaccgcc
    31861 caaaagaatg aggaagacgc ctccggctac gattacattg cgtaaagact tgtccatcac
    31921 tccatctcct gtcaattagt cttaaattta tatgcaccgc agcagtcgcg cttacggaaa
    31981 agctggaaag caaactgatt acctttgtat ggaaatgctt tgccgctgtc tctgactaat
    32041 gttgaacgtc caataggctc acagcaacgg cctctgaatt ttcgggcggc caccggataa
    32101 gtcatagcca ttttatagtt ctgtttatcc attacgggat taagtttgta cccgcataaa
    32161 gcatccctgc ctgatgtcct ccaagctact aagtctctgt gcatttttgt ctgaattcta
    32221 gccgccatca atttagcggc ctgcacttcg gagaagtggc cggacatcca tcctgacaaa
    32281 gggtaaatcg ttccgtggca acctgcacac caatacaatt tattgaatgg aaagccggcg
    32341 gccgctttaa cacagtcagc cgcacaggct gcttgtgcaa ccacattggc aaagagcgca
    32401 gaatcagggg cttgataaac ggccaatgac ggatatttcc aagtgggatc aagttccgtc
    32461 atccaaccga tatccaaagt acctttttcc atacaatcgt tgtccaataa ggcttccaac
    32521 caaaatacga ccggattttt ataccaatga acttgataaa aggcattatg ctgatcatgt
    32581 tgttcgttac gaccacgctt atgcgagccg ccaaaaagat tttgaatctt attggatacg
    32641 tctgctttac ctgttgtttc cgccgtatct gctttttgat ttttaagtga aataccgcct
    32701 aggctaacca tacagaaggg ggtgcgtacg acttctacca agtgtgtggg ttcccaaaat
    32761 gataccgtca cgcctgaacg gctcgggtgc gtacacgaac aaaaccaatt gtttggagaa
    32821 gagccgttat cctcttgatt atccatattc agattgattt tgccgccgat tttgattggg
    32881 aacatacagc tccaacaaat atcatttatc ggattaagca tcttcccctg gcaggttaat
    32941 ttattcggta tcggagcagt aggaacagct tcag
//
LOCUS       DAXFHF010000020        31192 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXFHF010000020 DAXFHF010000000
VERSION     DAXFHF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31192
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.020"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..1189)
                     /locus_tag="ACFIRM_000832"
     CDS             complement(<1..1189)
                     /locus_tag="ACFIRM_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC3028500.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGEAFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAQTGSGGSMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIR"
     gene            complement(1186..2169)
                     /gene="patB"
                     /locus_tag="ACFIRM_000833"
     CDS             complement(1186..2169)
                     /gene="patB"
                     /locus_tag="ACFIRM_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC3028501.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(2180..3616)
                     /locus_tag="ACFIRM_000834"
     CDS             complement(2180..3616)
                     /locus_tag="ACFIRM_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC3028502.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(3746..4911)
                     /locus_tag="ACFIRM_000835"
                     /pseudo
     CDS             complement(3746..4911)
                     /locus_tag="ACFIRM_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            5353..5565
                     /locus_tag="ACFIRM_000836"
     CDS             5353..5565
                     /locus_tag="ACFIRM_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC3028503.1"
                     /translation="METLLLDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            5712..7291
                     /locus_tag="ACFIRM_000837"
                     /pseudo
     CDS             5712..7291
                     /locus_tag="ACFIRM_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            7374..8471
                     /locus_tag="ACFIRM_000838"
     CDS             7374..8471
                     /locus_tag="ACFIRM_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC3028504.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            8585..9700
                     /locus_tag="ACFIRM_000839"
     CDS             8585..9700
                     /locus_tag="ACFIRM_000839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC3028505.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTAGNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNPASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVPPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGKGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSFAEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGYLLNMPKI"
     gene            9768..10280
                     /locus_tag="ACFIRM_000840"
     CDS             9768..10280
                     /locus_tag="ACFIRM_000840"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC3028506.1"
                     /translation="MPSERVSASGGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            10357..10833
                     /locus_tag="ACFIRM_000841"
     CDS             10357..10833
                     /locus_tag="ACFIRM_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC3028507.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            10821..11747
                     /locus_tag="ACFIRM_000842"
     CDS             10821..11747
                     /locus_tag="ACFIRM_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3028508.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            11812..12369
                     /locus_tag="ACFIRM_000843"
     CDS             11812..12369
                     /locus_tag="ACFIRM_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3028509.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            12477..14543
                     /locus_tag="ACFIRM_000844"
     CDS             12477..14543
                     /locus_tag="ACFIRM_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3028510.1"
                     /translation="MLKCCQPNPTQPNPTQPNPTQPNPSPDLSDTAGANTEAIYTSDG
                     ITANSTQLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLL
                     RNLPPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYN
                     TGSDGFVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIA
                     RELLKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDY
                     EWIFVFAKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDP
                     KTGKKYPANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAG
                     EDFGKVAVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFT
                     FPKPSQLIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEK
                     TAEKSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDK
                     DFSPEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYL
                     LNKGFTSADLLAFIKKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIEL
                     NVIIRV"
     gene            14633..17572
                     /locus_tag="ACFIRM_000845"
     CDS             14633..17572
                     /locus_tag="ACFIRM_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC3028511.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(17666..18625)
                     /gene="ttcA"
                     /locus_tag="ACFIRM_000846"
     CDS             complement(17666..18625)
                     /gene="ttcA"
                     /locus_tag="ACFIRM_000846"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC3028512.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(18717..19580)
                     /locus_tag="ACFIRM_000847"
     CDS             complement(18717..19580)
                     /locus_tag="ACFIRM_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC3028513.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAK"
     gene            complement(19615..19977)
                     /locus_tag="ACFIRM_000848"
     CDS             complement(19615..19977)
                     /locus_tag="ACFIRM_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3028514.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            20052..20531
                     /gene="ybaK"
                     /locus_tag="ACFIRM_000849"
     CDS             20052..20531
                     /gene="ybaK"
                     /locus_tag="ACFIRM_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC3028515.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            20553..21245
                     /locus_tag="ACFIRM_000850"
     CDS             20553..21245
                     /locus_tag="ACFIRM_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC3028516.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIIQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            21249..22124
                     /locus_tag="ACFIRM_000851"
     CDS             21249..22124
                     /locus_tag="ACFIRM_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC3028517.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            22332..25760
                     /locus_tag="ACFIRM_000852"
     CDS             22332..25760
                     /locus_tag="ACFIRM_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC3028518.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAVTIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGMTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            25975..26958
                     /locus_tag="ACFIRM_000853"
     CDS             25975..26958
                     /locus_tag="ACFIRM_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC3028519.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            27229..28719
                     /locus_tag="ACFIRM_000854"
     CDS             27229..28719
                     /locus_tag="ACFIRM_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3028520.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRRDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(28787..30184)
                     /gene="aspA"
                     /locus_tag="ACFIRM_000855"
     CDS             complement(28787..30184)
                     /gene="aspA"
                     /locus_tag="ACFIRM_000855"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC3028521.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(30260..30397)
                     /locus_tag="ACFIRM_000856"
     CDS             complement(30260..30397)
                     /locus_tag="ACFIRM_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028522.1"
                     /translation="MAEHNPPKDILAAGGACRKVRKVTTENNVSRFQTAYDMDNFIRP
                     V"
     gene            complement(30461..31021)
                     /locus_tag="ACFIRM_000857"
     CDS             complement(30461..31021)
                     /locus_tag="ACFIRM_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC3028523.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT         7734 a         8051 c         8518 g         6889 t
ORIGIN      
        1 gcctgattgc ggcggcgcgg acgagttctt cgaggctgtc ggcaagcatt tccgccgcgc
       61 gccggtagcc ttgggcggag aagtgtacgc cgtctttggc ggcccatcct tggttgagcc
      121 agtttttcat gctgcatatg ccgcccattg cgttttgcca agaccaaaac atcgtctgcc
      181 cctgacgggc gacgcgccgc tgcatctgtt ggacttcggt caggaggacg gggcgcgtgc
      241 cgcatacgcc gagcgtgttt ttcagggatt cgggcgcgcc gatgatgagg atgccggcgg
      301 cgggcaggct gtcgcggatt tggcggacgg tatccagcca tttttgttcg gtatcggcaa
      361 tgtcgatgtt gttgttgaag gcttcgttgg tgccgtagga aaggataacc aaatcggcgc
      421 cggtttgggc aaggtcgttc atacggtcgg cacgccattt cgaccactgg gtcaattgtg
      481 cgccgttgat gcccatcgcg gaaacggtaa tgccgccggc gggattttcg atgttgatga
      541 agccgatgtc ccacggcatt tcggtctgta tggccagggg cagtgccgcg cccgtatcca
      601 gtacctgcca gccgccgccg ttggcggaga cggtgttgcc gttgacggtc agggtttgtt
      661 cggcgagcag cggtttggca aacagggaaa cgcgctgttt gccggttttg ccgtcagacg
      721 cggtcagggt catgctgccg ccgctgccgg tttgggcgag gatgccgccg agggggaagt
      781 ctgctgcggc acttcggctg ttgaggcttt gccagttgcc gttatagcgg acggccgcca
      841 tgcgctgccc tttgacgttg gcggggtaaa cccagcctat gccgccgtcg ccccatgttt
      901 tttgcaggcg tttgcgcagg gcgtcggtaa agaagtcgcc ggcggtatgc gagtcgccga
      961 tttgcaggat gcggaaggcc tcgccgctgc cttgtgcgac ggatcggagt tttttcatcc
     1021 aaggcgaggc ggcggcgttg ccgtagtcgg tcagcagccc gtttgtatcg gtgtaggggg
     1081 cggacgggga aacggtaacg gtgtcggggc tgacggaggc gacgggcagg gcttctgcct
     1141 gcgtggcggc gaacagggcg gaaaatgcga tgaagtgttt ggggttcatg gctgtgtact
     1201 tgatggttgc gtactcggtt caaaaacgat tttttccatt attttttccg ccagcagttt
     1261 ttgtccttcg gcggtaaagt gtatgccgtc cttactgcgg tagcggacgg gtttgccgtt
     1321 gacgttgacg gaatcggtgt agcggccttt cccgccgctc agtgtttgcg cggtgggaat
     1381 caggatgatt ttgcctttca agtgttccga aagcagtttg tcgaggtagc gcatctgacc
     1441 gtcgagcttg acttttttca tgtaggggat gccgagccag acgacttgga cgcggtgcgt
     1501 gtgtgcggct tcaaggatgc ggtcgacgcg tttcaggtat tcttgcgccc attcgtcgga
     1561 agcgaatttg aggtagcgtt tgccgacggg gaaatcccac gggtcgttcg ggccgaggaa
     1621 gacggcgagt acgctgattt cgggatgttt tttcaaggtt tcttcaatcg ttttcggcca
     1681 gtcgaagaat gagggatagg aaagccccgt gctttgtttg ctgaggttgg cggattcgat
     1741 gccgtattgc tgtttcaggc ttttttgcac gaagggcgca acgccctgca tcagcgaatc
     1801 tccggcgaaa aagactttgt cgccgctgcg gacggcggcg gcttcggtgt cttgtttcca
     1861 ttctgttccg ccggccggcg gggcggcttc ggatgcggcg gcttcaggcg gcatatctgc
     1921 cgaaccgccg tcttgagccg tcggcggcgt ttcgccggac aggaaggttt tgatgccgtc
     1981 tgaaagggcg taggcgtttt cttgcaacgc cgctccgctc cgccaccatc cgtaggcggc
     2041 aagcggttcg agcgggctgt tgcggtggta ggtctgctgc cagtaggcgt tgatggggtt
     2101 ttggctgaac cacacggcaa tcagggcaga catcagtatg gaggcgaaaa gggaaagaaa
     2161 gtttttcatg tccaaaccct taaaaattgg cataaataaa gccgggtatc cccgaagggg
     2221 cgaggacgat aatcagcagc aggatgatgg aaatctggat aaaccacagc cacatcggga
     2281 ttttttccaa acctttgacc gtgccgtcga aagcgcgttg caggtaaggg tagagcagca
     2341 tcaaggatgc aaacgaggca agcagcagca tatccgcccg ttgcggcgcg ttccagccgc
     2401 cggcattggc aaagagggca ctgaaaactg cgcctgcatc gtcggggttt gcggtattga
     2461 agacgacaaa gctaaggcag acgaaatgga aggtaacaaa ccacgagagc ggcgcaaggt
     2521 atttcagacg gcatagcgcg tcgcgtccga aataccggtc gcccgtgttg agcagcgcca
     2581 gtgccgtgcc gtgcagcgcg ccccaaatga ggaagttcca gccgtagccg tgccagatgc
     2641 ctgagagtac cattgccgcc atcaggttga gctgtgtccg caaaaagcct tttttgctgc
     2701 cgcccaaggg gatgtagatg tagtcgcgta tccaggtgga aaggctgatg tgccatttgt
     2761 cccaaaatgc gcggatattc gcggcacgaa gcggtgcgga gaagtttttg ggcagccgga
     2821 agcccagcag catcgccata ccgataacca aatcggaata tccggaaaag tctaaaaaga
     2881 gttggaaggt atagccgtac acgcctgcca acacgcccca gccgtcgaat tgggcgggat
     2941 tttcaaatac gggcgacacc cagttttccg ccagcatccc cgccagccac cattttttgg
     3001 caatgcccag taaaatcagg gaaacggcga gggcggggcg gacgggcgaa cgcggtcggc
     3061 gggtgcggat ttgcgccagt gcacccgcct gttcgccgtc cgtgcttttg aatgcggcgg
     3121 cgcggataat cgggccggag gtaacggtgg ggaaaaaact caggtgcagc agcagctcgt
     3181 gccacccaaa acgcgcggcg tgcggggcgc ggaagcagta aaccagatag gcgacagact
     3241 ggaaggtata ataggaaagc cccagcggca tcaggatgtc gattgcgccg ccttttccgg
     3301 catattgtgc aatcagcgga cggaaaaaat cgaaatattt gaaaaagccc aaaacggtaa
     3361 tcgaggcggc aatgccgcac cccagccaaa agcggcgcgt actttcgcga tcggaacgga
     3421 gcagttcgcc caaaaggtac acgcaggagg aataaaggac gatgattgcc gcaaatacgg
     3481 ggctaatatg gtagagccag cccataccgg cagccaaaag cagcaggttt tggacggacg
     3541 ggtatttcgc aaagccccag taaatcggca ggaagacgat aaaaaacagt gcgaactcga
     3601 cagacagcag cggcataaac catccttggg acaaaaaaat aggcgttaaa acacggcaaa
     3661 gcccgttatt ttaagcaggg gcggatattc cttcaatcaa aatgccgtct gaagggcggg
     3721 aggggcttca gacggcatcg ctcggtcagg ttcggatatg gtggttcaaa ttcaggcaga
     3781 cccgcaaacc tgccgccgcc gtcagcatac ccgcaacgcc gatgcaggaa atgcccgagt
     3841 attgcgtaac ctaatgtccc agcagtgcgc cgccgcctat accgacgttg taaagccccg
     3901 aataaatcga gttggcaagg tctgcggcat cggaggcgaa atccaaaact ttggcaacca
     3961 tccccaagct gacgaccaca atcgccgtcc cccatacgaa caccaacgcg taaatggcgg
     4021 caggaaaatg cgccaaaggc agcaaaagcc ccgaagagag ggcgataagc gacaccgcgc
     4081 ccgccaaaaa tgcgcgcgga tgtttggcaa accatttgcc gaacaggtaa gacgcggcaa
     4141 aacccgccag gccgtacagc cccggaacca ccgtaacctg ccgcgcggag aaaccgccga
     4201 tttggatgac gaagggctcg atgtagctgt atgcggtaaa gtgcgccgta atcaccggca
     4261 ccgttaccgc atacagcagc atcagttttt tccgtttcaa aagcaggggc aggctgctta
     4321 aagaaccggt attcacgctc ggcaggcgcg gcaggctttt ggcaagcact gccatcaccg
     4381 ccgccgaaca caggccgatc agcagaaaac tcgcctgcca gcccaaatat tgtccgacca
     4441 tacgcccgag cggaatcccc gccgccatcg ccatcaccgt ccccgtgctg agcagcccca
     4501 atgcctgatt cccttttccg acaagggcga tgcgtaccgc cagcgaagcc gtaatggacc
     4561 agaacaccgc gtgcgtcagc gcaatcccca cgcggcttgc gagtaggatt tcaaaacgcc
     4621 acgaggcaaa cgacaggatg tggctgacag taatacagcg caaacaggac cagcagcagg
     4681 ccgcgccgtt ccataattgc gcgtcagcag catcagcggc agcggcagcg aagtcagcgc
     4741 gacaatccac gcataaaccg taatcatcac acccgtttcg gtcgccgcca tgccgaagct
     4801 tcgtccgatg tcgctcaaaa gcgcgatggg gatgtattcg gccgtgttga aaataaatgc
     4861 gccgattgcc aaggcaacga tgctcaacca aggtttgtgc gaagcgttca tctcaattcc
     4921 ttatatgtcg caccccgcca aaagggggcg cattatggtg aattaaattt aaaccggtac
     4981 ggcgttgccc cgccttagct caaagggaac gattccctaa ggtgctgaag caccaagcga
     5041 accggttccg taccatttgt actgcctgcg gctcgccgcc ttgtcctgat ttttgttaat
     5101 tcactatact tttcttgtct gccggacggc aaaatccaaa tcagggggaa gcagccgcct
     5161 cccgttcgga gaaacactcg ggacgctgct ttgaaaatcg ttccatatgt tgaaacattg
     5221 ccgcctcccg attgggcaag gttgccatta tgtcgcaagc atccgtgccg caccgtttcc
     5281 gcgtttgccc aagttcacgc tccgacttac aataacgccc gtcagattgc agtaccaacg
     5341 aaaggaaaaa tcgtggaaac cctcctcctc gacatcggcg gcatgagctg cggcggctgc
     5401 gtcaaaagcg ttacccggat attagaaagc gtaaaaggcg tggcaagcgt cgaagtcagc
     5461 cttgaaaaca aaagtgcgac cgtcggatac gaccccgcgc aaaccgcagc ggaagcacta
     5521 atcgaagccg tagaagacgg cggctatgat gcggcgttga aataaagcgg caaaatgccg
     5581 tctgaagcct ttgcgtttcc ccgatgccgt ctgaagcacg ctccgcgcct tcgggcggca
     5641 tttgatttag ttgaacgggc aggatgaatt ggttaaactt gcgggcaata gtgccttcag
     5701 acaaacaaaa tatgccgaac aaaaagaaaa aagccgcctt tgcgcgctgc aacggagaac
     5761 ggtgtgccgg gcgtgtccga catcaaaaat tcatatgatg atttgatgta cgaatccaaa
     5821 gcattttccc aaaccgccat caacgccttg gaggcatgcg cccgcatgat gggtttgaag
     5881 cctacagcag ccgcaaaagc caaggtcttg gagctgggct gttcgatggg cagaaacatc
     5941 atcaacgcag gcactttatt acccggatgc ggaatttgtc ggtatcgact tgtccggcag
     6001 gcaggttgcg cagggcaacg ccatcattga aaaaatgggc ttggaaaatg tgcgtctgga
     6061 agaaaaagat attttgaccg tcgatgaatc attcgggaag ttcgactata tcatcgtcca
     6121 cggcatttgg tcgcgggtgc ccgacgcagt taaagacaaa atcttttcga tttgccggaa
     6181 caacctgacc gaatacggca ttgcctatat ttcatacaat gtttaccccg gttggaagcg
     6241 gcaggagcag ttgcgcgata ttatgcagtt tgccggcagg gatgcgcttg gagaaccctt
     6301 ggaagcaagg acgcggaaag gcttggatgc gatcaaggcg ctggcggaaa ttttggaaaa
     6361 cgacaagggc ttgggcggcg gcaaacttcc ggcgattcaa aaaatattga atcacaatac
     6421 ttattatgtc gcacacgaat atatggaaat attcaacaac ccgatttacg ttaacggttt
     6481 cattgaatgg gcaaaccgcc accggctggc gtatatcggg gataccgatt tgcatgtgtc
     6541 ttttgtttct tggatggcgg agcatacgcg ggagcggatt ctggcattgg cagggggtga
     6601 ttatattgcc aaagaatttt acagcgatat tttatccgac cgccaattcc accgttcgct
     6661 tttatgccgt gaggaagtcg gggatactgt caggcgtgat gagtcggttg ccgtcgaagt
     6721 gatagaaagt ttgaatttcc gcccggcaag aggggaaaca atcaactttg atgaaaacga
     6781 taccctgctt tcgggcatac gcgatgtgat gaaaaccgga gaggcgttta aaacggaaga
     6841 tgttgcggaa aatcttgccc gccgatttcc cggtctggaa tttgaccgca tgaaaatcaa
     6901 ttcccagctt ttattgcaaa ccattctcgg gcgtttttct gtttcatcag acaatgcggg
     6961 caaaccattt tttgaagacc ataaaaccta tgtgccggcg cgctttacaa actatgccgc
     7021 cgcctttgtg gaacacggtg cgggagcgtt tgtccggcct gccaaccgtt acaacgaaag
     7081 cacgccctca ttcggatacg ggcatttgta cattatgcgg caattgtcgc ggccgacgag
     7141 caaacaggca ttgattgaaa cggttgccga aaacttgaac atcgtcagcg ccacgcccga
     7201 cggtttgaca ttccatccgc ctgccgaagt gtatgtggaa gaaatattgg cagacttggc
     7261 agacaggcat tttctcgttt cggcggattg acgggcaagt cccgacggca atccgtccga
     7321 tatattttca gacaggaaag acacacactg cgaaaaacgg tacacgacac attatgaacc
     7381 agacatttac cttgcccgat acgcgcccgt atccccaaaa tccgattaaa aaccacctgc
     7441 tgctcaatgc ctaccagttg gcgcacaatt cgtcacaggc ttcgcgcaaa ctctcgtccg
     7501 ggcagcttca aaccgaaatc agggggatgc ttgagcaaaa ccactatatc aacctttccc
     7561 tcgcgctgac gatgtcgccc gatgccggaa cttatgccgc gctgctttcc agtgtgaacg
     7621 cggtactcga ttgcgagaaa gaaggcgaag tgcagtggtt cgccctgccg gtcgtgctgg
     7681 tgtccggctg caagaaagaa cgggcaatcg agatgaagct gccgacggag gcattgtttg
     7741 cctgcctgca aaactatccg cacctgcgcg cgttgacgca agagacacaa tggctgcctt
     7801 atcttgtgca ttcttccgat ttgagtgcgg tcgcgccgga tgagtggtgg cgtgccaaac
     7861 aaaataccga agcggcggcg caacacttac gccgtttcgc cccgcgccct ttgctgttgc
     7921 ccgaagggca gtccgtccac gtcgtttacg tgctgggttt cggcagcggc aaagttcagg
     7981 cggcattggg gcagaacctt cttcaggcag gcttgccctt aatgcaggta tggcaggaaa
     8041 atcttgcatc ggaaggcatt acgctgtttg ccaatccgct ttcccccgat accccggcgc
     8101 gcgcgctttc agacggcagc cacacgcgcc aacgtatggc gatggatgtt tttgcggcaa
     8161 acgcgatacg cgccgtccgg atgcagggtc cgcgcgtcgg cgtggtcgct gcggcaaagg
     8221 cgggcggaca gattctattc ggatttaatg cgaccgacgg cgcgtttgaa gtcgtgccgc
     8281 aggtgttttg ctggcagctt tcctttaccg ataacattgc cgtcatccag cagaatttcc
     8341 tcgacctgat ggcagaatgc cgtgtagaac acgtttacct gctgcacaat cccttgggcg
     8401 aacaggaaaa catcccgagc tatgcggaag cgttgaaacg ggaagggcgc aatccgtttt
     8461 tcagcgcata gtgatttgta gtagtgatag gctttctcat ttctataata aatcaaaata
     8521 aagagcacaa cactttttca ttctgtgttg tgccttgagt gaaacgaaag gatgaattat
     8581 aaatatgaag attgtaattg caccggattc gtttaaggaa agcttgacag ctcaacaggt
     8641 agctgaagca ataaaaagag gcttccaaca atcgatagca gatgtggaat gtctcctctg
     8701 tcctgtcggt gatgggggag aaggcactgt agatgctatc cgacattctc ttgacctaga
     8761 agaaaaatgt ctccaagtga caggaccttt tggacaaaaa gaagtcatgc gctattttca
     8821 aaaagaacaa ctagccctat tcgaagttgc tgatttggtt ggtcttggaa aaatcccgct
     8881 agagaaacga aatccattac aaatccaaac tcgtggtatt ggagaattga ttcgccacct
     8941 cattagtcaa gaaattaaag aaatctatat tggcgttggc ggtacggccg gtaatgatgg
     9001 aggtattggg attgctgctg gtttaggtta tcaattttat gatgaggatg gaaatgcctt
     9061 acccgcttgc ggtcaatcct tactaaaccc agcttctgtt tcaacagaaa atcgctatga
     9121 aattcctgaa gatgttcaca ttcgtatttt agcagatgtt gtgagtccct tatgtggaca
     9181 ccaaggtgcg acctatacgt ttggcaagca aaaagggctg gattctacta tgtttgaggc
     9241 cgtagatcag gcaatacaag atttttatga aaaagtcccc cctgcaacat taaaacttaa
     9301 aggagcagga gctggtggag gcatcgctgg tggtttgtgt gcctttgctc aggcaagtat
     9361 cgtatctgga attgacacct gcctggactt gattgacttt gataagaaag tgtcagatgt
     9421 tgacttggtt atcgttggta aaggaagact agatcgtcaa agtttagcag ggaaagcgcc
     9481 tattggtgta gcaaaaagaa cccctgtcgg agttcctgtt gtcgctattt gcggcagttt
     9541 cgctgaagat ttgccttccc taccatttga aaatatccaa gctgcctttt ctattttgga
     9601 gaaaagtgaa cctttagaag atagtttgaa aaatgccagt ctttatttgg agcatacggc
     9661 ttctaatatt ggatatttat taaatatgcc taagatttag ccaagccatt tcttccagat
     9721 ggatgttttg gcaggttctt ccttatgcgg ttacgattaa ccggccgatg ccgtctgaaa
     9781 gggtttccgc ttcaggcggc atcgcgttgg aacagggatg ggatatgaaa aaaccgaaaa
     9841 tcctttttgt ctgcctcggc aacatctgcc gttcgccgat ggcggaatac attttgcgcc
     9901 gccgtgccgc cgaagcgggc attcccctcg aaacggacag cgcagggaca tcgggctggc
     9961 acgacggcga ggatatgcac cgtgaaacgg caaagatatt gaaaaaatac ggcattgacg
    10021 cttcaggctt taccagccgc aaaatccgcc aaagcgatgc gacggcgttt gactacatca
    10081 tcgctatgga cggcaagaat ttgtccgaat tggaaaaaac tttcggcagg cggccggaaa
    10141 aaatattcaa gctgaccgac ctgatacccg aaagcggtta cgaccatgtc cccgatccgt
    10201 ggtacatggg tgattttgaa gaaacataca ggcttgcgga tgcaggctgt cgggcattat
    10261 tggaaaagat ttccaaataa agcaattgaa tacaaacaca aatataaaac cgcctcctgt
    10321 cgtatcggtc gttcggacgg cataaacagg gaatttatga aaacaaattt caaacagaaa
    10381 attatcgaac aggcacgcag agaggacttg caggtaaccg ctttgcgcga gcaggtatta
    10441 gatattgtct tgcagcaaag cggcgtgatt aaagcctaca atgttttgtc acagatgcag
    10501 cagcagagcg aaggcgtgct tgcaccgcct accgcctacc gtgcccttga tttttgggcg
    10561 gatcagggcg ttttgcacaa agtggcggcg gtcaacggct atattttgtg cagccacgcg
    10621 cagcacgagt gcaacgacca ttgccacgac cacgaagaag ccgaagcgca ccacagcgcg
    10681 tttattttgg tctgcaccga atgcggcgcg gcggacgagc aaaccctcag ccacgagtgg
    10741 gcggcactgc gtgcaggcgt tgccgaaagc ggttttgcgc tgaaagaaga acacgttgtt
    10801 ttaactggaa tctgtaaaaa atgtcagaag tgaaaaaaac caaagtccac ctgatttcag
    10861 gttttctggg aacaggcaaa accaccgcgc tcaaaagcct gatggcgcaa aaagacccga
    10921 acgaaaaatg ggtcatcatc gtcaacgagt tcggcgaaat cggcattgac ggcgcggtat
    10981 tgagcgacaa cggcatccct gtggcagaaa tcgccggcgg ctgtttatgc tgcaccgccg
    11041 gcccgcaaat gggcgtaacc gtgcagaaaa tgctgcgcga cgccaaaccc gaccgcctga
    11101 tgattgaggc aagcggactg gcgcacgccg ccagcgtcat cgacgaattg aaagccaaac
    11161 ctctggacag tcttttggaa atcggcgccg tctttaccgt cgtcgatccg cgccagttca
    11221 tcaatcccga ttacgcgcaa caggcgttgt ataaagacca aatcggcatt tgcgacgtat
    11281 tggtcgccag caaaaccgat ttatgcaccc ccgaacagct tgccgaattt catgacaaag
    11341 cggcaaaact gttcccaccc aaagccaaag tggtcgaagt ccaaaacgca caactcgata
    11401 tccaatggct cgatattccc gtagttgaaa aatcacgcta ccgcctcaaa gccctgccgg
    11461 acaacaccat gggcttccag tcgcaaggtt tcacattccc cgccggacgc gatttcgacg
    11521 gcgaaaaatt gaccaacttc ttcaatgatt tgcccaaaat gaccgaagga ctcgtccgcg
    11581 ccaaaggcgt gttccaagta ttgggaacgt gggtgtggct taactgggtg gacgggcagt
    11641 ggggcgcgaa ccaagtgtct tggcggcgcg attcgcgttt cgagctgatt gccaaatcgt
    11701 ttgacgcgga tttaatcgaa caaaaactta aagacgcatt ggaatagcgt tcacgcgctt
    11761 tcagacggca tatgaaatga aaataccgtc tgaaggcttt aggaggacgg gatgaaaccc
    11821 aaattcaaaa ccgttttaac cgcgctgctt ttggcggttt ccctgccgtc tatggcggca
    11881 acccgcgtcc tgatggaaac cgatatgggc aatatccgtt tggttttgga cgaatccaaa
    11941 gcctccaaaa ccgttgccaa tttcgtgcgc tatgcccgaa aaggctttta cgacaacacg
    12001 attttccacc gcgtcatcgg cggcttcgtc atccaaggcg gcggattgac cgaggacttg
    12061 gtgcaaaagg caaccgataa ggccgttgcc aacgaatccg gcaacggctt gaaaaacacc
    12121 gtcggcacca tcgcaatggc gcggacggca gaccccgatt ccgccgccgc ccaattcttt
    12181 atcaatctgg cggacaacgg ttcgctcgac tacaaaaacg gacaatacgg ctacaccgtt
    12241 ttcggcaggg tagaaagcgg aatggacacc gtttccaaaa tcgcccgcgt caaaaccgcc
    12301 acgcgcggct tttatcaaaa cgtacccgta cagcccgtca aaatccgccg cgttgttgtc
    12361 gggcagtaac acgcagacag acgttcagac ggcgtcgccc gtttcccaaa aaacgccgtt
    12421 taaagtaaaa aaatatttta aaacagacag ttgatattga caaattcaaa ccgaggattt
    12481 taaaatgctg ccaacccaac ccaacccaac ccaacccaac ccaacccaac ccaacccaac
    12541 ccaacccaag ccctgatcta agcgacacag ccggggcgaa cactgaggca atctacactt
    12601 cagacggcat taccgccaac tctacccaac tcgaacagct caaaaaactg tttcccgcct
    12661 gttttgacgc agacggaaat ttccttatcg acagattaca agccgaaatt gcgccacaga
    12721 ccgacatcgg acgcgaattt tacgaaatga actggctggg caaatcatat gcccgcctgc
    12781 ttcgcaacct gccgcccgaa accctgataa gtgaagacaa aacgcacaac gccaagcccg
    12841 aaaatgcagg cagccaaaat ctgctgattc gcggcgataa tctggaagtg ttgaaacact
    12901 taaaaaacgc ctacgcaaac agcgtgaaga tgatttacat cgacccgccc tacaacaccg
    12961 gatcggacgg ctttgtctat caggacgacc gcaaattcac acccgccgaa cttgccctcc
    13021 tggcgaatat tgacgaagac gaagccgcgc ggattttaga tttcaccgac aaaggctcaa
    13081 actcgcacag cgcgtggctg acctttatgt atccgcgcct gtatatcgcc cgcgaactgt
    13141 taaaggacga cggcgtgatt tttatctcga ttgacgataa cgaagcggcg cagttgaaat
    13201 tgttgtgtga tgaagtgttt ggggaaggga attttgttgc acaattgcct tggcgaaaaa
    13261 gaacagctaa atcagatgtg ccttttggta tttcgcagga ttatgaatgg atatttgtat
    13321 tcgcaaaatc ttgccaattt attgcagcaa ctaaaggcaa ggaacgacgc tattatgaga
    13381 ctgatgattt ccccgatcgt ccttggcgta cccacgactt aacgaaacaa acaactgcgg
    13441 cggaaaggcc aaatagtttt ttcacaatgg ttgatcccaa gacaggaaaa aaatatccag
    13501 caaatccaaa tgcaacttgg cgtgtaacca aagatacatt tcaagattat tataataaag
    13561 gaaaaatcgt tttccctgat gattatgatt tcttaaatat cagcaatcca gttatgcgtt
    13621 actttaaaga tgacgacatg aaaaaagctg gcgaggattt tggcaaagta gctgtaagca
    13681 gtagattacc tgaaaatgtt ggtacattag ccgatgcagt agccgaatat ttggctattt
    13741 ttagtaggac gctacctgaa aatatcggaa tgactaaaga aggcacaaaa gaaatcacag
    13801 atttatttgg tagcaaaata tttaccttcc ccaagcctag tcaattgatt aaatttttag
    13861 tttcaataag ttcaaagagt aatgacctaa tcctagactt cttcgcaggc agcggcacaa
    13921 ccgcccacgc cgtgatgcag cttaacgccg aaggacaaaa cggtaaccgc cgctatatct
    13981 gtgtacagct tcccgaaaaa accgctgaaa aatccgaagc ccgtaaagca ggctacccga
    14041 ccatcttcga catcaccaaa gcccgcatag aaaaagccgc cgccaaaatc cgcgtcgaac
    14101 atcccgatta cacgggcgat tcgggcttca aaatctttca aacggcagac aatttcaggc
    14161 agcatccaga caaggatttt tcgcccgaac aaccggattt gccgcttaac gatgaattaa
    14221 gcgaagaaca gctgcaaacg cttctgacca cctggacgct gtatgacggg gcggcactga
    14281 ccacgccggt tgagcctgtg cggttgggca gttatacggc gtatctgtgc gaaaaacggc
    14341 tgtatctgct taacaagggt tttacttccg ccgatttgct ggcgtttatc aagaagctgg
    14401 acgacgatgc ggatttcaat cccaaccgtg tgattgtatt cggcagcaat atggcaagcg
    14461 ccatgcagca cgaactcgac caggcggttc gcggttatac caataaaaaa gagattgagt
    14521 tgaatgtgat tatcagggtg taaccggaat ccgaaagtgc aggctgcttt tcggcttgga
    14581 ttccccttgc gggggaagga cgatacaaca atgacttcat ggagaaacat tcatgagcgg
    14641 ttttaattac gagaaaaacc agccgcacca aatgcgggcg gtttcggcgg ttttgggcgt
    14701 gtttgacggg gcaacgccca aatatcggac ggcagacgaa aatcccgaac ttttgtttgc
    14761 tgcaaaacaa tacgcaaaca atatcctgaa agtgcaaagc caaaacggta tagacggccg
    14821 attccccgac cgttcggacg accaaaatat ccttgatatt tccatggaaa cgggcacggg
    14881 caaaacctat acctacacac aaaccatgtt cgagctgcac cgttggctgg gcgtgttcaa
    14941 atttatcgtg gtcgtgccga ctttgtccat taaggcggga acacagcagt ttttgcaaag
    15001 caaggctttg gcagagcatt ttgaacagga tttcggcggc gattatgaag gcgtacgcct
    15061 gaaaacctat gtggtggaaa gcgcgaaaaa gaataagggc aaaaagtcca atgcgcccat
    15121 aacgattgag caatttgtca aagcggaaaa caaaaaggaa attcatgtgc tgctgattaa
    15181 cgcgggcatg gttaattcgt cgtccatgaa cgatacgggc gacaaggcat tgaaggattt
    15241 gtttgacaat cccgttgatg cattggctgc cgtgcgcccg tttatgattg tggacgaacc
    15301 gcataaattc ccgacccgag atagcgcgaa aacgtggggc aatatcaaac gcttaaaacc
    15361 gcaatatatt ttgcgctacg gtgcaacatt taacgatgaa tattacaact tgctttaccg
    15421 tttgacggca gtagacgcgt ttaacgacgg gctggtcaaa ggcgtgcgcg tgtttcagga
    15481 agaaatgcag ggcggcatgg atgcggcggt aaaactggtg tcgtcggacg gcaaagaagc
    15541 gaaatttgaa ttaaacgaaa aggacaaaaa gcagacgttc aaactggcaa aaggcgaaga
    15601 tttggcgcaa atccatccgg ctatttcgga tttgaaaatc gacaaaatga ataaaaccgt
    15661 ggtggtgtta agcaacggct tggagttgaa aacgggtgcc gtcatcaacc cttattccta
    15721 ttcgcaaacg gtgcaggatg cgatgatgca gcgggcggtt gccgaacatt tcaagctgga
    15781 acgcgcgctt ttggcagaac gcgcgccaca gcccaaaatc aagccgctga cgctgttttt
    15841 tattgacgat atcgcgggct accgcagcgg caacgagctt tcaggcagcc tgaaagataa
    15901 atttgaaagc tggattcgcg cggaagcggc acgccgtctg aaaacggaaa gcgacccgtt
    15961 ttaccgcgat tacctgcaaa agacgttgga cgatgtatcc gcctgccacg gcggctattt
    16021 ttccaaagac aatacagaca gcgacgatag aatcgagcag gaaatcaatg aaatcctgca
    16081 cgataaggaa aaactgctgt ctttggacaa cccgcgccgc tttatttttt ccaaatggac
    16141 gctgcgcgaa ggctgggaca atcccaacgt tttccagatt tgcaaactgc gttccagcgg
    16201 cagcacgact tccaagctgc aagaagtcgg acgcggcctg cgcctgccgg taaacgagct
    16261 gatggcgcgg gtgcgcgatg taccgtacaa actgaattat tttgtcgata gcagcgaaaa
    16321 agactttgtg aagcagcttg tcggcgaaat caacgacaat tcttttcagg aagaaatctc
    16381 caaaaagttt accgaagagc tgaaacaaaa aatattgcaa aaataccccg atatcaaacc
    16441 gctggtattg gtaaaccaac tgttttcaga cggcatcatt gacgacaatg aaaactttgc
    16501 cgaagacggc tatgacaaat taaaagccgc ctatcccgaa gccttcccca aaggtttgga
    16561 caaaggcaaa gtcagcaacg ccaaagacga gggcaaagac accatcatca tgcgcgaagg
    16621 caaatatgaa gaactcaaag ccttgtggga gctgattcac cataaagccg ttttgcagta
    16681 caaaatcaaa gatgaagccg aatttgtcga tttatttacc gcctatttgc gtgaaaacgc
    16741 cgccaaattc ccgcaggcag gcatatgcac ggcggtaaac gaagcttata tcaacaacgg
    16801 gcttatgctt tcccgccgca tagacagtat tgaagatgaa gattttatcc gtttcaacac
    16861 aatgacttac cgtgagtttc tggaaaaact ggcacaaacg gcaaaaatcc agatgcagac
    16921 tttgcatcag gcgttttacc gcgtccgcga cgaactgaac attggcgatt ttttgaatat
    16981 gcagaccatc gcccaaatca aaaacggctt caaccggttt ttgcttcatc attccttcca
    17041 taaattcgaa ctggattacc ggcttgtcgg cagcaaaatc catccgacca aatttaccaa
    17101 taaagacggc aaaccgcgcg cggtgaaaaa agcagatttg ggcagatttg aagatacgga
    17161 gcaccggcct gccgccggct atctcttcgg cgagattttc tacgattcgg atatagaaca
    17221 tgaaaatgtc gccaacaacc aaattgaagg cgtaatcgta tttaccaaaa taccgagaaa
    17281 ctccatcaaa atccctgttg ccggcggcgg cacgtattcg cccgactttg cctatatcgt
    17341 gaaaaccaaa agcggcgaga ttctgaactt tgtgattgaa gccaaaggga ctgacggggc
    17401 ggaagatttg cgaaaaagcg aagagcggaa aatcaaacat gccgaaaagc tgtttgccga
    17461 gatttccaaa gaaatcaagg tggtgttcaa aacgcagttt gacggcgaga ggatagccga
    17521 actgatcggg caaaatatgc cagcaggcgg gcattctgaa aacggacact gagaaacaaa
    17581 tgtttggaag ccgtctgctt cgggatttta aaatgccgtc tgaaacgcca aacgggcttc
    17641 agacggcatt tttgacggtg gaggtttacg cgccgcaggt tttcggcttg ttcgccagaa
    17701 tgttgatgac tttgcgttcg gctttttgcg gctcgatttt gatttcgctc tcatcttctt
    17761 cgctgccgtc tgaaaagcgt tcgggcattt tttcgctgtc aaaagccaaa tcgccgccgt
    17821 gtttcagatt ttgaccgcgt tccaatccgg caaagtcaaa gagttcggta tcggcaaggt
    17881 gggaagggac gacgttttgc agggcggaga acatcgattc gatacggccg gggaagcgtt
    17941 tgtcccaatc gcgcagcata tcgccgatga cttggcgttg taggttgggc tgcgagccgc
    18001 agaggttgca cgggataatc gggaactgtt tcaattcggc gtatttgatt aagtcttttt
    18061 ctttcacata cgccagcggg cggatgacga tgtgttcgcc gttgtcgctc accagcttgg
    18121 gcggcatggc tttgagtttg ccgccgtaaa acatatttaa aaacatggtg gcgaggatgt
    18181 cgtcgcggtg gtgtcccaag gcgattttgg tgcagcccaa ttcttttgcg gtgcggtaga
    18241 ggatgccgcg ccgcaggcgg ctgcacagcg aacaagtcgt tttgccttcg tccaacacgc
    18301 gtttgacggt ggagtaagtg tcttcttcaa cgattttgta gggaacgccg atgctttcga
    18361 gataggtcgg caatacttct tcggggaagc ccggctgttt ttggtcgaga ttgacggcga
    18421 ccagttcaaa atcaatcggc gcgctggctt ggagccggcg caggatgtct aacagggcgt
    18481 agctgtcttt gccgccggag aggcagacca tgattttgtc gcccggctcg atcatgttga
    18541 aatcgttaat cgtgtcgccg acggcgtggc gcaggcgttt gctgagtttg ttgttttcga
    18601 gttcttgctt ggtttttttg gacatggcgg tggtttgaaa aattagggat gcggcattgt
    18661 aaccgaaaac aggggcggca atgccgtctg aagggcttca gacggcatta gagggcttat
    18721 tttgcatttt tggttttaaa gaagagatga accgctttga agataccgcc gtttgggacg
    18781 gtgagttctt tttgcgcggc ggagaattta atcacggcaa gggcggtaaa gttttccgaa
    18841 tcttgaacgc tgtcgagtac gatgccggct tcttcgccgt ccgccgtcag caggatgccc
    18901 gcttcggctg ccgaatttcc cgacaatacc gccaagccgc gtttgacctg cccccgatac
    18961 tgggcgcgcg cgatgatttc ctgtcccggg tagcagcctt ttttgaagtg tacgccgccg
    19021 atgatgtgct ggttgagcat ttgggcgacg gcggtttctt tggtagccgc gcatatccac
    19081 ggataaccgc tgcggatttc gtgcagccgc cacgcgtttt cggcggcagc gtcataaggg
    19141 ggtagggcgg tttcgggggc gatgtgcaaa atgccccgat ggtgcaggat gacggaacag
    19201 atgccgtctg agacgcattc ggaggtaaag gcgagactgg gttcttgcgc ggcaagcggt
    19261 tcggcggatg ctgccaattc cgcgccgacg gcgtaatctt caaggatttc aaaaacggct
    19321 ttggcgcgca acacaaacat ccgcaggcgt ttgaccgttg cttcaagcaa atcttgcgcc
    19381 ataatcagca gcaaatcgtc tccacggttg acgacaatca tattggcgat gacgcggcct
    19441 ttgggcgtgt tgtaagtcgc gtaacacgcc tgcccggtct gaagattatt gatgtcgttg
    19501 gaaagctgtc cgtgcaggaa ggtttggcgg tcttcgccgc tgacgcgcgc cacgccgaaa
    19561 aagggcagta aggttttcat catttgcgta ctctgaaaat ataaggaaat ctgtttatgc
    19621 agttgccgcg tctctcttca cggcggttat tttgatttcg acgcgccact ccggacgggc
    19681 gagccgcgct tccacgcagg cgcgggcggg cgttctgccg gcggcaaccc aagcgtccca
    19741 cacgccgttc atttccgcat agtcgcccat atcacgcagg tagatgaccg catccaaaac
    19801 gtgtgccttg tccgagccgc attccgccag ccagcggtcg gtttgggcaa gcacgtcggc
    19861 agtctgttcg gcagccgttt caccgttttc gggaaccatg ccggagagga aaatcaagcc
    19921 gcccgcgcaa accgcttcgg aatagcgggg cgttgtgccg aaataccgga tatccatatc
    19981 ggtttccttc gataaagggg atatatggta acattgcgct tgaccgattt ccatgttttg
    20041 catgacgaaa aatgagtaaa cacacttatc cgataacacc tgccgtacgc gttttgcgtg
    20101 aaaacggcat cgaatttgaa ccttttacct atgcctatga ggaacacggc ggcacggcgc
    20161 agtttgcccg actgttcggc aaagacgaac acttggtcat taaaaccatt gttttgcaag
    20221 atgaaaacgg taaggggccg attgtcttga tgcacggcga caaacagatt tcaacccgca
    20281 atctggcgcg gcatttgggt gcgaaacaca tcgaacccgc cgcgcctgcg caggcaaaca
    20341 agtggacggg ctatctggtc ggcggcacaa cgccgttcgg catccggaca aaattggata
    20401 tttacgtcga acagtcggtg atggatttgg aaaccatcta tatcaatggc ggaaaacgcg
    20461 ggttcattat cggtatccgc cccgatgatt taaatatttt gaacccgaaa acaatacagg
    20521 cagcggtttg accgggaagt ataaggagca atatggacaa agatttgtat gccgtattgg
    20581 gcgtgtcgcc gcaggcgggg gcggacgaaa tcaaacgcgc ctaccgcaag ctggcgatga
    20641 aataccatcc cgaccgcaat ccgggcaatc tgcaggcgga agaaaagttc aaagaaatcc
    20701 aacgggctta cgatacgctt tccgacctgt caaaacggac gcaatacgac gcgtccttca
    20761 gacggcatga ggaacgcggg cggcaggaag aggcattccg ccgcgaacag gcgcgcaggg
    20821 agcagtttta cggcgaacag atgcgccgcg aacaggcgtt cagacgggag tttgaacggc
    20881 aggcttcacg ttcgcgccat gcttacgaac cgtccggcag cggaagcggg cgcaactata
    20941 tcctcgccgc ctacatcctg ttcggtttgg gtgcaatcat acagttcatg cccatagtcg
    21001 gcgtgattct cgcctatatg aaacggaaca gtttggacag cattgtctat gccgcacata
    21061 ccgaatacct gattaaaacc ttttggcgca cattttggct ttatcttttg ggcgcgctga
    21121 ctgcccgttt cggcatcggc gtgctggtta ttattgcaac gaccgtctgg tatttctacc
    21181 gcatcatcgc cggctttatc cgcttcaacg acggcagggc ggttgcgccc gagaaatgga
    21241 tatagagcat ggcctacctg ttaatcagca tcgtgttcag cgtgtcggtt tccattttgc
    21301 tgaaaatggc aaggaagaaa aaaatcgaca tcgcgcaggc ggtcgccgtc aattatgtgg
    21361 tcgcggtcat actgaccctg tcggtattga agccggatgt cggcaatatc ggtgcatttt
    21421 tgccgacgtg gccgctgttt gccgctttgg gcgtgctgct gccgtccgta tttgtcatta
    21481 tgggcaaatc cgtagaatcc gccggtatcg tcaaatccga cgcggcgcag cgtttgtcgc
    21541 tgtttttacc gattgttgcc gctttgacgc tgtttggcga aaaactcagt gaaggcaaac
    21601 tgatcggact gtgcctcgcc tttgccgcac tgttctgcct gctttggaaa agcgacagcg
    21661 gcaaaaaatc aggcagcgcg tggcggcagg cgttattgct gctcggtgtg tgggcgggtt
    21721 acggcattat cgacatcctg ttcaaacaga ttgccaaaag cggcacggca tttgcgggca
    21781 acctgctggt tgcatttgcg ctggcgggtg tgctgatgtt tgcctgcctg tttgccaaat
    21841 cggtcagatg gcgcgttgag agcgtggtcg gcggcatact cttgggcggt ttgaatttta
    21901 tgaacatcgt aacctacatc actgcgcacc aaatgatgaa ggacagtccc acgctggttt
    21961 ttgccggtat gaatatcggt gtgattgttt tagggacgct ttcgggcgca ttgttcttta
    22021 aggaaaaaat caacacaatc aatacggcag gcattgtact gtcattatgc tccattgcct
    22081 gcctgttcta ttggacagaa gttaaggcat tgttcggcat ataatccggc tgattgatat
    22141 aacaaaatgc cgtctgaaac aggaaaatgc tgcttcagac ggcattttgt tatacgggca
    22201 gattcaaaat ttaattaata atagttattt ttaattataa tttaaatatt aataataatt
    22261 aaaaatttca aataattctt attatgagtg tatcttaata aaattcattt tatattctta
    22321 ataaggaata catgatgtcc ggattctccc ccaaacctaa gaccattatc ctaagccttg
    22381 caggtgcatt tggcgcattg gcttttgcag acaccccgaa caataccgaa cagcagaaag
    22441 aactgaatac catagtcgtc catggcaaac gcagtgccga ccaaaaaggc gcggacgacg
    22501 tgtattacaa aaacgtctcc aacgcctacg tcggcaaaga atacctcgaa cgctaccgcg
    22561 tccaatccgc cggcgacgta ctcaaaggcc taaacggcgt gtacaacatg aacacccgca
    22621 ccgccggcgg cgccatcacg cccaacatac gcggtatcac cggcaagggc cgcatcccgg
    22681 ttaccataga cggcaccgaa caaaccatcg acgtatggat gaacaactac ggcgtgggcg
    22741 accgcaacta cctcgacccc gccctgttcc gcagcatcgc ggtggaaaaa agccccgccc
    22801 tcacgcgcgg cgtgaaatcg ggcgtcggcg gtgcggtcac catccgcacc atcgaagccg
    22861 gcgacatcgt gcccgaaggg cagaaattcg gcttccagct gaaaaccgag tttgccaaca
    22921 acagaacccg cccggccaac aacctcaacc aatggctggg ctgggaagac taccgcaccc
    22981 tgccgctcgg cgccaccgcc gacggcgcgg gcggcggttc tgacccgatc accggacagc
    23041 agagcccgca ggcgttggtg gtagacaact ttaccccgcc gcagcacaaa agcggccgcg
    23101 acaactggcg cttcggcggc gaccgcagtt atatggccgc cgccgccttc aaaaccgaac
    23161 tttcagacgg ccttgccgcc tacagctacc gcaacaaagg caactacttc gccggtcgca
    23221 agggtgcgga aggctacctg aataatccgg tatacgacct gcaaaaatgc tacgacgaag
    23281 gcggcagcga tttcaactgc aaaaattccg ccaccttcat cccaaatatg gcgcgcatct
    23341 accaccccgg ggtggaagtg ctcaacagca acaccgaaac caaaaccctg ctgctgaaaa
    23401 acaactggca cctgcccggc agccatcggc tcggctggca atatatgcgc accgacgtgc
    23461 gcttcggcga gatcaacccc ttccacacca cctatgtgat gaatatggaa gagcacaacg
    23521 agaccaaccg ccccaaagaa ctgtcgccac aggcgcagag catcgattcc accatccgca
    23581 ccgacaccta caaactcggc tgggcctgga agccggaaaa cagccgctgg gtcgatctgc
    23641 aggccaacct gtggcgcatc aaaaccaaca gcacccgcca ccagagtggc ggcatggatt
    23701 tgtcttccgc ccgccccgat cccttctacg acgtttggta ttggtgtacc caacgcggcc
    23761 ggataccgcc cgagcacgtc gacaactaca gcagctgcaa cgacctgatg aatgatttcg
    23821 gcgtcaacgg cctgaccaaa gaacaggtgt tggccatgac cccgaacgac aacggccaat
    23881 tccgcgtgat ttccggcgcc gagcagaaaa cccgcgtcag ccgcaccggt ttcgatatca
    23941 gcaaccgctt ccgcctcaga gaccgcctga gcatgacctt ggccgccgat tatcagaaag
    24001 agaaactggc ggaggaagtg gaaatcgtca attcgcaaga cctgttcaac ctggccggca
    24061 tggtaaccgg catgaccaag ctggccgggc cgcgcggcgg cgagcggcgc gaatggggca
    24121 ctaatttggt gttcgactgg caggccgccg accgcctgaa aatctccgcc ggcatccgct
    24181 accacaactt ccggggcttc gataccgccc tggccgaagg gcgtgcccgc cgcgacccgc
    24241 gctatcaggc aggcgatgga acccagcatt atgccgatgg tgcctatctg ccgtatttcg
    24301 aactggcagg cgatcaggag cggcgtgact ggaatgtggt ggccgaacag cttcggcagg
    24361 cgtatcagtc aggcgatgct gccgccattg ccgcggcagt acaggcagaa caggcgcacg
    24421 gcgcccgtta ccatttgctg aaatactatg acggctcgta tcgtaccgat ggttatgtgt
    24481 atatcggcaa cgatggccgt gcctattttg acggacggtt cggcggggtt aacgaggcac
    24541 agaaccaacc gctctatcgg gtgcgccccg tttatgtgcc gtttgtcaac ggcaagctgg
    24601 acagcggcgc attgccccag cattttcatg atttcgtcag caattatcag gaaaaagtga
    24661 ccaatccgca ggggctgcac gggacgcatt atcgatattg ggcaggcgtg gggaatccta
    24721 tcgactgtat aagaagtgat gcggcctctt gccttgctca taacctatcg ggaaaagtaa
    24781 ccaattttca agtcggctat cccgagtacg gtgatgatac cggtctcggt ctcaaaatca
    24841 tcggtcggca ctataccgag gaacagtatt gggcgcagcc caaaccgatc cgtgcccatg
    24901 cctgggcgcc gaccatcgcc gtcagctacg atctgaccga caacagccgc ctgttcgtac
    24961 gctatgcgca gatgagccgc ttccccagcg tgtatgaggt gggcagtttt tataacgacg
    25021 tggcctatgc cggtatgccg aaggccccaa atttccgctt caagccggaa cgcagccgca
    25081 gctgggaaat cggctacagc ttcaattttg ccccgtattg gagccggctg cgtgccggcg
    25141 atatgcgcct gacctactac cgcaaccgta tccaaaatgt gatcgaaacc acagattatt
    25201 tccgcaccgt gcaatacgac cgcaaggaca ccgccgggct ggaatggcag agccgcatcg
    25261 acaccggccg cttctttgcc gcgctcggtg ccacctaccg cctgaaacag cagatgtgtg
    25321 accgcgatac cgctttcgat tacgaccctt atggtgccaa aggcgtgccg atctgcatcg
    25381 aaggcggcta cggcagtacg cgcggctatc aggccttgca gccgaaatat tcaataaacc
    25441 tggatgccgg ggtgcgcctg ttgggcgaga aactggagct cggcctgcgc ggcatctacc
    25501 acagccgggt caacaccaag caatacgacc aactgctgca aaaggatttg ggcatcatct
    25561 tcgataccac cggcaagccc caccattggc ggtcatcgct gacttgggat gtttatggcc
    25621 gctaccggct gcacaaaaac ctcaacgtca acctcggcat caccaacctg accaaccgct
    25681 actatctcga cccgatgtcc aacgttgccg cccccgggcc gggccggacg gttaccttcg
    25741 ggctgactgc caagttctag ccgcagggcg gagtggggtt ttcaggtagc ctgcgggtat
    25801 ggcgggaagg ctacctgaaa acggattgga tataacaaca aacaattgaa cactccggac
    25861 gcacaaaccc aaccggcccc gcttatccga ggtaatggtt tttaccgttt tttccgccgg
    25921 ttcggcggta ataaaacggt ggcttttgtt taactcttaa ttaaggaaac gattatgtct
    25981 atacgtaata aaaccctgtt ggccgtcttg ctgtgcggcg tttttggtgt ggctgcggct
    26041 gctccggctg gtggttccag ccaaaggaaa agccaagatc caaatagtga cccgacgaca
    26101 gttcgccccg gcacctctga caagcaacct ttcggtgccg gtaaggccgg tttggcattc
    26161 agtatcaata acggtggaag ttggtcgaaa tccatcaatc tggatgatgg tccggtcaaa
    26221 tatatccgtg cgaaatatca ggcaccgatt aacggcaacg atccctatgc taatgttgat
    26281 ggtacgcgca atctgcaata tctgcgctta ggcgatgtga aaccggttat cggctggttc
    26341 tacaacccaa cgttgggtca ggtgtggtat gaaaaccacc acaacaacac tgaagtgtac
    26401 agtgtgcgcc agatagccaa cccggcaatc cccgccgcac ccaagttcgg cggcctggta
    26461 atcgccaaag tgccgggcat gggcggcagc aacggcaacg tcttcttcgg cgaatgggcg
    26521 ccgcgtgccg gtaacccgca gcaaatcagc accgacctga atatggcgga tggtaaacgc
    26581 accgtttggt atgtgggcga taatcccacc ggcaacacca ccggtgtgag aaccgccacc
    26641 tacaatgtgc tgggtatcaa taagcacacc ccggggcaga acgatttcta taccggtcag
    26701 gtaaccgcca ccttcggttc tggtataacc gggacgatgg caggcagaat cagccgcagt
    26761 tccgatcacg tcgattttga tcacgccaaa atcgataaca gaaatggtac ctttgccgat
    26821 cccagtcaag gcatgaccgg ccgcttctac ggcaaccagg cggcagccat ggccggctat
    26881 accacacgtg gtaccggtaa tgtcggcgat gacgtagctt tcggcggtag cgccaccgat
    26941 attgagaccc gcaaataaca gctgccatcc gatttggttt ggcaacggct aggctggatt
    27001 taacccctga ggagtttcaa gctgccgttt tatgccgact gccaagccat ataaatcatc
    27061 cgtccgatac ggattgctgc tcggtttcag gtagcccgtg ttcaacacag gctacctgaa
    27121 atttgatatt tgtaaataaa catcgcctat cccatacggt ttcggcctat cggacatttt
    27181 gggacagcaa ttaaaacctg cgttacttta tcttttcgga aggcaaccat gaacaaacca
    27241 ctcagcctcg ccctcttctg cctgcttctg cccatcaccg cccacgccga caaccccgcc
    27301 gacgaacgcc gccgcctgct ggacgagggc agccgccaaa cgcagcaata ccgcgaaagc
    27361 ggttggctgg acacggaaca ggcacgcggg gaagtagagg aaaacgacgg ctatatttcg
    27421 ataggcggcg aaatctacca agtcggcgat acggcggaag aactggaaag cgcaatttac
    27481 cacgccctca atgcgcggca gtggcacaag gtgcggcagt tcgccgcccg ctacgccaaa
    27541 ctgccccggc acaaacccgc gctgattcat ttggccgatg cgctgcaaaa acgggacgaa
    27601 ggggatttcc gcgcggcggg gaacagtttt cagacggcct tggaagccga acccgacaac
    27661 ccccgcctgc tgctcgaagc ggggcggttt tatgccgaag acaaccaaaa caaagagtct
    27721 gccgccgcgt ttgagaaagt tctgaaaacg gacattcccg cagaaacccg ccccatcgtg
    27781 gaaaactacc tgtccgaact gggcaaacgc cgccgctggc acggccaaat cagcctcggc
    27841 tacggctata acagcaatgt aaaccaaggc aacggcatca accaatgcgt gtgggaaatt
    27901 gcgggcatgt gcctgatgga gcgcaccctg cccgcgccaa cggattcgac cttctcatcc
    27961 tatagcgcga ccgccgaaaa aaccgtcccg ctcaaagggc atcacggcgt acaggtgcgc
    28021 ggtgtactgt acggcaaccg ctacacggaa aaagacaaag attcggcggc catgccggat
    28081 tacggctatc gcaacggttc gctgtacgcg ggatacgcct atgccgacgc acgctcctcc
    28141 ttttccctgc tgccttattt cgaatacgac ttccgcaacc gccacaccca ctaccgcgca
    28201 tggggtgcgg atgccgactg gtcgcgcaca ctctccccgc actggcgcat taactcacat
    28261 gccggagcaa aaaagaccgg atacggcgga caaagcaaaa cttattttgc cgatttcaaa
    28321 caatacgaac tcggcgcggg ggcggaattt tccatcacgc cgaaaagcgg cctgcttgta
    28381 aatttcgatg ccgcacgcaa agcctatccc gaaaaatcct cttccagcaa agaatatacg
    28441 gctcggctgg gcgcgtatag gcttttttcg ggcggtactt atttaaacgc cgtcctgctc
    28501 taccgccgca gcctttatga cgcggcaagt ttcgtcagcg ataacaaacg gcggcgcgac
    28561 aagcaataca tcatgatggc tgcggcgggt tttccgcaat ggaatatcaa aggcgtatat
    28621 cccgaactgc gtttcaggcg aacaatcgca cacagcaacg cggtgtatta ccgctaccgc
    28681 caaaacgaat ggctgttggg ttttaaatat cggttttgac cagtcgggtg ataaaaaatg
    28741 ccgtctgaaa gggcgcttcc tttcagacgg catttgtccg taatgtttac agatgaggat
    28801 tcatcaggtt ctcgggggag aggatgcggt tgagttcttc ttcgctcaat aacccgcgtt
    28861 ccagtacgac ttcgcgtaca cctttgccgg tctgggcgca gattttgccg accaagtcgc
    28921 cgttgcggtg gccgatgtac ggattcagat aagtcaccag cccgatggag ttgaaaacgt
    28981 aacgctcgca gatttcgcgg ttgaccgtaa tgcctttgac gcatttgtcg gataggttga
    29041 ctgcggcatt gcccaagagg gaaatggttt caaacataca ttgggcgatg accggctcca
    29101 taacgtttaa ttgcagttgc ccggattcgg cggcgaaggt aatcgtcgta tcgttgccga
    29161 tgactttgaa gcagacttgg ttgacgactt cgggaatgac gggattgact ttggcaggca
    29221 tgatggaaga accggcctgc aattcgggca ggttgatttc tttcaaaccg gcgcgcggac
    29281 cggaagagag aaggcgcaaa tcgttgcaga ttttagagag tttgacggcc gtgcgtttca
    29341 atgcgccgtg taccatcaca tatgcaccgc agtcggaggt cgcttcgatc aggttttcag
    29401 tcagtttgca aggcaagccg ctgacttcgg agagtttttt gaccaccagt tcggcgtagc
    29461 ctttgggcgt gttcacgccc gtgccgattg ccgttgcgcc caaattgact tccaagagca
    29521 gttggcgggt gcggtcaagg ttgaggattt cctcttccaa caatacttgg aaagattgga
    29581 attcctggcc tgccgtcatc ggtacggcat cttgaagctg ggtgcgaccc attttcaaaa
    29641 cgtctttaaa cgcttcggct ttggcggcaa aggcgttttt caatacggtc agtttgtcga
    29701 gcaattcgcc gatgctgtaa tacacggcaa ggcggaagcc cgtgggatag gcatcgttgg
    29761 tggattggct ggcgttgacg tgatccatcg gattgacgat gtcgtagcgg cctttctcat
    29821 agcccaaggc ttccaatgca aggttggcga tgacttcgtt ggtgttcata ttgaccgaag
    29881 ttcccgcacc gccctgatac acgtcggacg ggaattggtc gaggcagcgg ttgttcagca
    29941 gaacttcgtc gcaagccttt tcaatggcgg cggcgatttc gggttttact gcacccaatt
    30001 cgccgtttgc ctgcgcggtt gcttttttca ccatcaccat actgcggaca aactgcggca
    30061 cgtcggaaat tttttgtgtg gagattttaa agttttcaat ggcgcgcagg gtgtggatgc
    30121 cccaatacac ttcggcggga atctcgcggt cgcccaataa atcgtgttcg atacggacag
    30181 tcatgttttt acctttgtaa gtcggataat taatattgaa aaaatgcgcc atcggaaaga
    30241 tgccgcagca ggacgaacac tataccggcc ggatgaaatt gtccatatcg tatgccgtct
    30301 gaaaacggga aacgttgttt tcggtcgtta ctttccggac tttccggcaa gccccgcctg
    30361 ccgccaaaat atctttcggc gggttgtgct ccgccatatc ggcttacggt tggcagggcg
    30421 gtttgatgaa gacggcattt gtgctgattc gcatcaaggc ttattgtttt gcagcttcga
    30481 tttggatgtc gatgcggacg tttttggtca taccggcgtt aacgaggtag tccacgcccc
    30541 atttggtgcg gtcgatggtg gtgctgaagt cgccgccgca aacttcggtt tccgccatcg
    30601 ggctttggta gcagttgaat ttttcggctt tgagtttgac gggggcggtt ttgccgcgca
    30661 tggtcaggtt gccgtcaacg gaaacaagtt ttttgccgtt gaagttgaat ttggtggaaa
    30721 cgaagcggat gtccggatat tgagcggcat cgaagatgtc ggcggatttc aggtggccgg
    30781 tgaagtgttg cgaaccgctt tgcaggttgg cgacgggaat ggtgatgtcg attttgccgt
    30841 cgcgttttgc ttgatcgaac tcgacggaac cggtcagacc gtaaaaaccg ccgacgttgg
    30901 tgctggtgtt gaagtggtcg atggcgaaac ggacgttggc gtgatattcg tccactttgt
    30961 aggtggcgga ggcagtgccg acggctgccg ctgcgagcgc ggcgaagatg atttttttca
    31021 tgatgataat cctttgtgtg ggccggtaaa ggcgtttatc ctaacatagg cggggattta
    31081 tggcatttcc tgccggtaaa cggtgggttt gcaggcggtt gacgaacggg cgggcggaac
    31141 agggcggatg aaaaagcgcg gtgttatccg tgctttgttt ttgggtctgt ac
//
LOCUS       DAXFHF010000021        30941 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXFHF010000021 DAXFHF010000000
VERSION     DAXFHF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30941
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.021"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..271
                     /locus_tag="ACFIRM_000858"
     CDS             <1..271
                     /locus_tag="ACFIRM_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3028524.1"
                     /translation="FRCIELPQSIRRVLPGAVNLFTRMIKTSSLAWLIGVIEVVKVGQ
                     QIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQKWEH"
     gene            273..1004
                     /locus_tag="ACFIRM_000859"
     CDS             273..1004
                     /locus_tag="ACFIRM_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3028525.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            1117..1971
                     /locus_tag="ACFIRM_000860"
     CDS             1117..1971
                     /locus_tag="ACFIRM_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3028526.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTAADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            2387..3208
                     /gene="prmC"
                     /locus_tag="ACFIRM_000861"
     CDS             2387..3208
                     /gene="prmC"
                     /locus_tag="ACFIRM_000861"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3028527.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVCGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            3294..4682
                     /locus_tag="ACFIRM_000862"
     CDS             3294..4682
                     /locus_tag="ACFIRM_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC3028528.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            4683..4907
                     /locus_tag="ACFIRM_000863"
     CDS             4683..4907
                     /locus_tag="ACFIRM_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC3028529.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(5307..6200)
                     /locus_tag="ACFIRM_000864"
     CDS             complement(5307..6200)
                     /locus_tag="ACFIRM_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3028530.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            6243..8945
                     /gene="ppc"
                     /locus_tag="ACFIRM_000865"
     CDS             6243..8945
                     /gene="ppc"
                     /locus_tag="ACFIRM_000865"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC3028531.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            9068..10057
                     /locus_tag="ACFIRM_000866"
     CDS             9068..10057
                     /locus_tag="ACFIRM_000866"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC3028532.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            10111..10440
                     /locus_tag="ACFIRM_000867"
     CDS             10111..10440
                     /locus_tag="ACFIRM_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028533.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            10451..10756
                     /locus_tag="ACFIRM_000868"
     CDS             10451..10756
                     /locus_tag="ACFIRM_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC3028534.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            10762..10941
                     /gene="ssrS"
                     /locus_tag="ACFIRM_000869"
     ncRNA           10762..10941
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFIRM_000869"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11119..11550
                     /gene="rplM"
                     /locus_tag="ACFIRM_000870"
     CDS             11119..11550
                     /gene="rplM"
                     /locus_tag="ACFIRM_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC3028535.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            11563..11955
                     /gene="rpsI"
                     /locus_tag="ACFIRM_000871"
     CDS             11563..11955
                     /gene="rpsI"
                     /locus_tag="ACFIRM_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC3028536.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12336..12830)
                     /locus_tag="ACFIRM_000872"
     CDS             complement(12336..12830)
                     /locus_tag="ACFIRM_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028537.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKEIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKELDSEKIIS
                     LNYD"
     gene            complement(13023..13952)
                     /locus_tag="ACFIRM_000873"
     CDS             complement(13023..13952)
                     /locus_tag="ACFIRM_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3028538.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            14081..14830
                     /locus_tag="ACFIRM_000874"
     CDS             14081..14830
                     /locus_tag="ACFIRM_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC3028539.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            14953..15534
                     /gene="petA"
                     /locus_tag="ACFIRM_000875"
     CDS             14953..15534
                     /gene="petA"
                     /locus_tag="ACFIRM_000875"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC3028540.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            15553..16902
                     /locus_tag="ACFIRM_000876"
     CDS             15553..16902
                     /locus_tag="ACFIRM_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC3028541.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            16917..17705
                     /locus_tag="ACFIRM_000877"
     CDS             16917..17705
                     /locus_tag="ACFIRM_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC3028542.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            18037..19827
                     /gene="rmuC"
                     /locus_tag="ACFIRM_000878"
     CDS             18037..19827
                     /gene="rmuC"
                     /locus_tag="ACFIRM_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC3028543.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALSNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(19894..20526)
                     /locus_tag="ACFIRM_000879"
     CDS             complement(19894..20526)
                     /locus_tag="ACFIRM_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC3028544.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            20619..21443
                     /locus_tag="ACFIRM_000880"
     CDS             20619..21443
                     /locus_tag="ACFIRM_000880"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC3028545.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRARTDR"
     gene            21512..22075
                     /locus_tag="ACFIRM_000881"
     CDS             21512..22075
                     /locus_tag="ACFIRM_000881"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC3028546.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            22159..22596
                     /locus_tag="ACFIRM_000882"
     CDS             22159..22596
                     /locus_tag="ACFIRM_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC3028547.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            22817..23086
                     /locus_tag="ACFIRM_000883"
     CDS             22817..23086
                     /locus_tag="ACFIRM_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC3028548.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            23086..24861
                     /gene="ptsP"
                     /locus_tag="ACFIRM_000884"
     CDS             23086..24861
                     /gene="ptsP"
                     /locus_tag="ACFIRM_000884"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC3028549.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(24988..25929)
                     /locus_tag="ACFIRM_000885"
     CDS             complement(24988..25929)
                     /locus_tag="ACFIRM_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3028550.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(25938..26813)
                     /locus_tag="ACFIRM_000886"
     CDS             complement(25938..26813)
                     /locus_tag="ACFIRM_000886"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3028551.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(26996..27072)
                     /locus_tag="ACFIRM_000887"
     tRNA            complement(26996..27072)
                     /locus_tag="ACFIRM_000887"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27036..27038),aa:Met,seq:cat)
     regulatory      27221..27320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27411..29312
                     /gene="thiC"
                     /locus_tag="ACFIRM_000888"
     CDS             27411..29312
                     /gene="thiC"
                     /locus_tag="ACFIRM_000888"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC3028552.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     gene            complement(29688..30035)
                     /locus_tag="ACFIRM_000889"
                     /pseudo
     CDS             complement(29688..30035)
                     /locus_tag="ACFIRM_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(30075..30428)
                     /locus_tag="ACFIRM_000890"
                     /pseudo
     CDS             complement(30075..30428)
                     /locus_tag="ACFIRM_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(30468..30851)
                     /locus_tag="ACFIRM_000891"
                     /pseudo
     CDS             complement(30468..30851)
                     /locus_tag="ACFIRM_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         7420 a         8259 c         8390 g         6872 t
ORIGIN      
        1 attccgctgc atcgagctgc cgcaaagcat ccgccgcgta ttgcccggcg cggtcaacct
       61 gttcacgcgc atgatcaaaa ccagctcgct cgcctggctt atcggcgtga tcgaagtcgt
      121 caaagtcggg cagcaaatta ttgaaaactc gttgctgacg cagcccaatg cttcattttg
      181 ggtttacggc ctgattttta tgctgtattt cttctgttgc tggccgctat ctctactggc
      241 ggcaaaactt gaacaaaaat gggaacactg acatggcttt actgagcatc cgcaagctgc
      301 acaaacaata cggcagcgta accgccatcc aatccttaga cttggacttg gaaaaaggcg
      361 aagtcatcgt actgctgggc ccgtccggct gcggcaaatc caccctcctg cgctgcgtca
      421 acggtttgga gccgcaccaa ggcggcagca tcgtgatgga cggtgtcggc gaattcggca
      481 aagacgtttc ctggcaaacc gcccggcaaa aagtcggtat ggtctttcaa agctacgaac
      541 tgtttgccca catgaccgtc atcgaaaaca tcctcttagg cccggtaaag gtacaaaacc
      601 gcgaccgtgc cgaagcagag gcgcaagccg gcaaactgtt ggaacgcgtc ggactgctag
      661 accgcaaaaa cgcctatccg cgcgaacttt ccggcggtca gaaacagcgc atcgccattg
      721 tccgcgccct gtgcctgaat ccggaagtca tcctgctgga cgaaatcacc gccgcacttg
      781 accccgaaat ggtgcgcgaa gtcttggaag tggttttgga actcgcccgc gaagggatga
      841 gtatgctcat cgtaacccac gaaatggggt tcgcacgcaa agttgccgac cgcatcgtct
      901 ttatggacaa aggcggcatc gtcgaatcgt ccgaccccga aacctttttt tccgcaccaa
      961 aaagcgaacg cgcccgccaa tttctggcag gtatggacta ctgatttacc gcaaaccgtc
     1021 cgaacccttt tcagacggca tttcataggg cagtgcttta tgtgtcgtcc tgtgtgttga
     1081 aacatcccaa gcaaacattc aaaaaggaga tttccaatga aattgaacgc caaactcaaa
     1141 gcccttttgg cttccgccgc catcgccgtc ggtctgaccg cctgcggggg cggctccggc
     1201 gatgcccaat cttcacaaag cagcggtgcg gcaaccgttg ccgccatcaa agaaaaaggc
     1261 gttatccgca tcggcgtatt cggcgacaaa cccccgttcg gctatgttga cgcaaacggc
     1321 aaaaaccaag gctttgacgt tgaaatcgcc aaagacctgg ccaaagacct gctcggcagc
     1381 cccgacaaag tcgaattcgt cctgaccgag gccgcaaacc gcgtcgaata cgtccgttcc
     1441 ggcaaagtcg acctcatcct cgccaacttt acccaaaccc ccgaacgcgc cgaagccgtc
     1501 gatttcgccg atccttacat gaaagtggcg ttgggcgtgg tttcccccaa aaacaaaccg
     1561 attaccgaca tggcgcaatt gaaagaccaa accctgctgg tcaacaaagg caccgccgcc
     1621 gacgctttct tcaccaaaag ccatcccgaa gtcaagctgt tgaaattcga ccaaaacact
     1681 gaaaccttcg acgcgctgaa agacggtcgc ggcgtagcac tcgcgcacga caacgccctg
     1741 ctgtgggcgt gggcgaaaga aaacccgaac tttgaagtcg ccatcggcaa tctcggtcct
     1801 gccgaattta tcgcccccgc cgttcaaaaa ggcaacgccg acctcctgaa ctgggtcaac
     1861 ggcgaaatcg ccgccatgaa aaaagacggc cgtctgaaag ccgcctatga aaaaaccctc
     1921 ttgcccgtgt atggcgagaa agtcaaaccg gaagcattgt tggccgaata agtttgtttg
     1981 acaagaaaat ccgtatccgc atttgaaggc gggtgcggat ttttttcagc cgccgaaaat
     2041 attgcaaatt caaaggaaaa atcaactatc tcatcgtttt atttatttta ttattcttgc
     2101 gcgtgcggaa atcaagttcg tccggttttg cctgtttttg aatttcggga aacttccaaa
     2161 tcgtcattcc agataaaacg cttcagcctt atgtttccgg atgaccgttt tcgcgggatg
     2221 acggcggagc gtttctgtta ttcggataaa ttcctacggt attgaaacgg cgataaggtg
     2281 ttttccttta ttatataatt tcaatgcaaa atgatatttt ttgaacaagc gtgccgcaaa
     2341 gccggaaaaa atgttcaagc cgcatcggcg gcagacggga cgaaaaatga cgtttgacga
     2401 atggttgggc ttgtcaaaac tgcctaaaat tgaagcaagg atgctgctgc aatatgtttc
     2461 ggaatatacg cgcgtgcagt tgttgacgcg gggcggggaa gaaatgccgg acgaaatccg
     2521 acagcgggcg gacaggctgg cgcaacgccg tctgaacggc gagccggttg cctatatttt
     2581 aggtgtgcgc gaattttatg gcagacgctt tacagtcaac ccgaacgtgc tgattccgcg
     2641 ccccgaaacc gaacacttgg tcgaagccgt attggcgcgc ctgcccgaaa acgggcgcgt
     2701 gtgggatttg gggacgggca gcggcgcagt tgccgtaacc gtcgcgctcg aacgccccga
     2761 tgcgtttgtg cgcgcatccg acatcagcac gcccgccctt gaaacggcgc ggaaaaatgc
     2821 ggcggatttg ggcgcgcggg tcgaatttgc acacggttcg tggttcgaca ccgatatgcc
     2881 gtctgaaagg caatgggaca tcatcgtgtc caacccgccc tatatcgaaa acggcgataa
     2941 acatttgtcg caaggcgatt tgcggtttga gccgcaaatc gcgctgaccg acttttcaga
     3001 cggcctaagc tgcatccgca ccttggcgca aggcgcgccc gaccgtttgg cggaaggcgg
     3061 ttttttgttg ttggaacacg gtttcgatca gggcgcggcg gtgtgcggcg tgttggcgga
     3121 gaatggtttt tcgggagtgg aaatcctgcc ggatttggcc ggtttggaca gggttacgct
     3181 ggggaagtat atgaagcatt tgaaataatt gtttgcaaaa ttggcggatt ttccgttatt
     3241 gggttatgat gtgttttgat atgtaaacat ttataggttt ggagcgataa acaatgaatg
     3301 cagtcgtagt tgccgtaatc gttatgctgg tgctgtcgct gtcgcgcgtg cacgtggtat
     3361 tgagcctgac ggtcggcgcg tttgtcggcg gcgcggtggc gggtatgccg ctgcaaaaca
     3421 ttgccgatgc ggcgggacag gtcagtcagg cggggattat ccccgtgttc aacaaaggtt
     3481 tggaaggcgg cgcgaagatt gcgctttctt atgcgatgct tggtgcgttt gcgatggcga
     3541 ttacccattc cggcctgccg cagcagcttg ccggcgcggt cgtccgcaag ctgaaccggg
     3601 gcggtatgcc cgacagcgtg cgttcgggcg agggcgcggt caaatggctg ctgctttcca
     3661 tcatccttgt gatgggcatt atgagtcaga acgtcatacc catccacatc gcctttatcc
     3721 cgatgattgt tccgccgctg cttttggtgt ttaaccgcct gaaaatcgac cgccgcctga
     3781 ttgcgtgcgt catcactttc gggctggtta cgacttatat gttcctgcct tacggcttcg
     3841 gcgcgatttt tttgaacgaa atcctgttgg gcaacatcca ttctgccgcg ccgcagcttg
     3901 atgtgaaaaa catcaacgtg atggcggcaa tggcgattcc cgcattggga atgctggccg
     3961 gactcctgct ggcgtttgtc cactaccgca aaccgcgact gtaccaaagc aacaatgccg
     4021 atacggcggg caacgccgat gcggcaaacc gtccgcagcc gtccgcctac cgcagcctgg
     4081 ttgccgccgc cgccatcgcc gtatgctttg ccatacagtt gatgtatgaa gattcgctgg
     4141 tgttgggcgc gatgctcggt ttcgccgtat ttatgatgtt gggggtcatt aaccgcgaca
     4201 aggcaaacga cgtattcggc gaaggcatca agatgatggc gatggtcggc ttcattatga
     4261 ttgccgcgca gggttttgcc gccgtgatga atgcgaccgg gcatattcag ccgctggtgg
     4321 aaagcagtat ggcgatattc ggcaacagca aaggtatggc ggcgttggcg atgctggtgg
     4381 tggggctttt ggtaacgatg ggcatcggtt cgtccttttc cactttgccg attattgccg
     4441 cgatttatgt gccgctgtgt accggtttgg gcttttcgcc gcttgccacc gccgccattg
     4501 tcggcacggc gggggcgttg ggcgatgccg gttcgcccgc gtccgattcc acgctgggcc
     4561 cgacgatggg gctgaacgcc gacgggcggc acgaccatat ccgcgattcc gtcatcccga
     4621 ccttcatcca ctacaacatc ccgcttctga ttgccggctg gattgccgcg atggtgctgt
     4681 aaatggacgc ggttcaagag ttggaacgcc gtattgtcga actggaaatc caaaccgcgc
     4741 ttcaggagga cgtaatctcc ggtctgaacg cgatggtggc ggaattgcgg cagacgttgg
     4801 atttgcagca ggctcagttg aggctgctgt atcaaaaaat gcaggacagg aatcccgacg
     4861 cgcaagagcc gtattccctg cgcgacgaga ttccgccgca ttattgatgc gccgccgtat
     4921 ccggatttcc ttaaataaag gctgtgtttg aatattccgc cggtgcaacc ctaagatgta
     4981 accttaatca gtcggacaaa atgcctttta tccgatgaaa ccggttcttg tccgaacggc
     5041 gatgagcggc ttcccccgct gcaaacagat aaaaccaacc gggtattcaa acacagccaa
     5101 aaaaagccgt ccgaacccaa aggggacaga cggccaaaca cattacgggg aaaacgtctt
     5161 actcaatgag tctgcgaaac agacattgct aaaacaactg caattataag gtaagtttat
     5221 ttttttgcaa agaaatgtaa atttttaaat ttttaaaacc gttccgccgc atccgttcgg
     5281 atgccgtctg aaacggcggc agggttttac cgtgccgcgc cgcaaaccgg gcattcgggg
     5341 ttgcgcggca ggtcgaaata ttgccagccc ccttccaagg cacggtaaac cgccagcctg
     5401 ccgtgcgacg gttcgcccgc ttccagcagg attttcagag cctccgccgc ctgtgtgcag
     5461 ccgacgatgc cgaccagcgg cgagagcacg ccgaagaggg aacagatgcc gtctgaagcc
     5521 gatccgccgt cgaacaggca ggcgtaacac ggcgagtcgg gcaagtcggg acggtatgcg
     5581 gcaagctgtc cttcaaaacg taccgccgcc cctgaaacca gcggtgtttt cgtttgcacg
     5641 caggcacggt tgaccgcctg ccgcgtggcg tagttgtcgc agcagtctaa aacgatgtcg
     5701 gcggtttgaa ccaaaccggt caggcggcag ccgtcgagtt tttcgttgac ggcgcggaca
     5761 tcgacagtat ggttgatgcg tttcaggcgg cctgccaagg tttcggcttt aggcttgccg
     5821 acatcgccct cgtcaaatgc gacttggcgt tgcaggttgt gcagctcgac cgtgtcggaa
     5881 tcggctatgg tcagcgtgcc gacgcccgag gcggcgagat agggcagggc ggcggcgccc
     5941 aatccgccgc agccgacgac caaaatatgc gcggcggaaa gcttctgctg cccttcgatg
     6001 ccgatttcgt ccaagaggat gtggcggctg taccgcagca ggagtgcgtc gtcgttgtcg
     6061 tgttcggtcg cggtcatgat gatgttcgga aaaaacagtt gcgggcgatt gtaacgccgc
     6121 cgtcgggcgg cgttcaactt cagacggcat ttcgggacat gagtggttaa agtgtgaacg
     6181 gtttggcacg gatgcggcat ttgaggtaca tttacagtat ttacggcaat gagaggaaaa
     6241 tcatgcaact gcatatcctg aacaatccga aggacgcggc tttggcggcg gacgcggaat
     6301 ttttgaaaca gtccctgttc aacctcctgc acgaagaagc ctcgccgttg gttgtcgaaa
     6361 cggtcaaact cttgtccact tccgacgaca gcgcggcatt gattgaaaaa gtattgccgc
     6421 aattggacga acggcagacc tacgatctga cgcttgcctg cgggctgttc gcccagattc
     6481 tgaatatcgc cgaagacgtg caccacgaac gccgccgcca aatccacgaa gatgccggcc
     6541 acaacgcagc agaaggcagc ctgacggaaa ccgtccgcag gctcaaagcg gggaaagccg
     6601 acggcaaatc ggtgcagcgg cagttggaca atacgtccgt taccgccgtt ttgaccgcgc
     6661 acccgaccga agtgcaacgc caaaccgttt tgaatttcaa ccgccgcatc cgcgcgctgc
     6721 tgccgcaacg cgaacgctgc accaatgccg acgcgctggc acggctgcgc cgcgaaatcg
     6781 acaccgtgct tctgggcttg tggcagacca gcgaaacgcg ccgccacaaa ctcagcgtca
     6841 acgacgaaat caacaacggc gtgtccatct tcccgatgag cttttttgaa gccctgccca
     6901 aactctaccg caagatggaa cacgactttc agacggccta tcccgatgtc cgcgttccga
     6961 acatccttaa aatcggcggc tggatcggcg gcgaccgcga cggcaatccg tttgtttctg
     7021 gcgaaaccct gcgctttgcc ttccgccgcc acgccgatgc cgtgttccgt ttttaccgca
     7081 gcgaactcga caaactttac cgcgaactgc cgctttccat ccgccgcgtc aaagtcaacg
     7141 acgacgtaat ggcactggca gccctctcgc ctgacgaaga aatcgcccgc accgaagaac
     7201 cttaccgccg cgccattgcc tacattatgg cgcgcgctat gggcaaagcg cgctcgctcg
     7261 gtttgggcat gggctgcaaa ttcggctttc tcgagcctta tgcttcggcc caaaaatttc
     7321 tcgatgattt gaaaaaattg caacgttccc ttatcgacaa cggcagccgc ctgcttgccg
     7381 aaggccgttt ggcagacctc atccgttccg tgtccgtgtt cggcttccac atgatgccgc
     7441 tcgacctgcg ccaacacgca ggcaaacacg ccgatgtggt tgccgagctt ttccaacacg
     7501 caggcttgga agactacaac agcctgaacg aagagcaaaa acaagccgcc ttgttgcgcg
     7561 aattgggcca ccaacgtccg ctgtacagcc cgttcatcac atacagcgac catacccgcc
     7621 gcgaattggc gattttcaac gaagcgcgca aaatcaaaga cgaatttggc gaagatgccg
     7681 taacacaaag cattatttcc aactgcgaac aacccggcga cctgctcgcc ttggcattgc
     7741 tgctgaaaga aagcggcctg ttggcggtgg aaaacggcaa accgcacagc cgcatcaaca
     7801 tcgtgcctct gtttgaaacc atcgaagcgc tcgaaaatgc ctgtccggtc atggaaacca
     7861 tgttccgcct cgactggtat gatgccctgc tcgaaagccg cggcaacatc caagaaatca
     7921 tgctcggcta ttccgactcc aacaaagacg gcggctacgt taccagctca tggtgtctgc
     7981 atcaggcgga attgggcttg gtcgaactct tcaaaaaata cgatgtccgt atgcgcctgt
     8041 tccacggtcg aggcggcagc gtcggacgcg gcggcggccc gtcttaccaa gccatcttgg
     8101 cgcaacccgc aggcagcgtt gccggacaaa tccgcattac cgagcaaggc gaagtcatca
     8161 ccgccaaata cgccgacccc ggcaacgccc aacgcaactt ggaaaccttg gttgccgcga
     8221 ctttggaagc cagcatcctg ccggataaaa aagaccctga tgcgaaactg atgcaggcat
     8281 tgtcggacgt atcgttcaaa tactaccgcg aactgattac ccaccccgac ttcatcgact
     8341 acttcctgca aaccagcccg attcaggaaa tcgccaccct caacctcggc agccgtcccg
     8401 ccagccgcaa aaccctggcg cgaattcagg atttacgcgc gattccgtgg gtattctcct
     8461 ggatgcaaaa ccgcctcatg ctgccggctt ggtacggttt cggcagcgcg gtggaaacct
     8521 tgtgcgaagg cagccccgaa accctcgccg ccctgcgcgg acacgcccaa aacaacccct
     8581 tcttccaagc gatgctctcc aatatggaac aagtgatggc gaaaaccgac atcaccttgg
     8641 cggaaaacta cgccggtttg agcgaatcac ccgaaaaggc aaaagtcatc ttcggcatga
     8701 ttaaggaaga ataccgacgc agccgcaaag ccctgctcga cctgctgcaa accgaagaac
     8761 ttttgcgcga caaccgcagc ctcgcccgtt cgctcgcttt gaggattcca tacctgaacg
     8821 cgctcaacgg tttgcaagtc gccatgctca aacgcctgcg caaagaaccc gacaatccgc
     8881 acgccctgct gatggtgcac ctgaccatca acggcgtggc gcaaggtttg cgcaataccg
     8941 gctgatagtg ccgcatcggg gtaaaatgcc gtctgaacgc ctttcagacg gcatttacct
     9001 gaccgcactt gcagagaaac accgattgtt ttaaagtgaa cggcagtgat atgttgaaag
     9061 acgaccaatg aaaattaccg ttatcggcgc aggttcgtgg ggtacggcgc tcgccctgca
     9121 tttttcccaa cacggcaacc gcgtatccct gtggacgcgc aacgcagacc aagtccgcca
     9181 aatgcaggaa gcgcgtgaaa acaaacgcgg actgcccggc ttttcctttc ccgaaacctt
     9241 ggaagtgtgt gcggatttgg cagaggcgct caaagacagc ggacttgtcc ttatcgtaac
     9301 ctccgttgcc ggattgagaa gcagcgcaga gctgctcaaa cagtacggcg cgggacacct
     9361 ccccgtcctc gccgcctgca aaggcttcga gcaggataca gggctgctga ccttccaagt
     9421 cttgaaagaa gtattgcccg acaataagaa aatcggcgta ctttccggtc cgagttttgc
     9481 acaggaactc gccaaacaac tgccctgcgc cgtcgtcctc gcctccgaaa accaagagtg
     9541 gattgaagaa ctcgtaccgc agctcaacac gaccgtcatg aggctttacg gcagtaccga
     9601 cgttatcggc gtggcagtag gcggttcagt caaaaacgtc atggcaatcg ctaccggact
     9661 ctccgacgga ctcgaatacg gactcaacgc ccgtgccgca ctggttacgc gcggactggc
     9721 cgaaatcacc cgccttgcct ccgcaatggg cgcgcagccc aaaaccatga tggggcttgc
     9781 cggcatcggc gatctcatct tgacctgtac cggtgcactt tcgcgcaacc gccgcgtcgg
     9841 cttgggtttg gcagaaggca aggaactgca tcaggtgctg gtcgaaatcg ggcacgtttc
     9901 cgaaggggtc agcaccatag aagaagtctt caataccgcc tgcaaatacc aaatcgatat
     9961 gccgattacc caaaccctgc tgcaactcat ccgcaaagaa atgacccctc agcaggtcgt
    10021 cgaaaggctg atggaacgca gcgcgcgttt cgaataaaca acaaacagat gccgtctgaa
    10081 cccccagacg gcatacagac aggtaaggtc atgaaacaaa atatcgaaaa actcgaaagc
    10141 agcgtttata cgttggtaca aaaattcgaa accctcgtca gcgaaaaccg ccgcctcaaa
    10201 gaaaccgtcg ccgaactcga acgggcgcac gagcggcaaa aactcgaaca cgagaccgcc
    10261 gtcgacgaac tcagcgaagc cctgctcgtc caagtcggca aactcaaaga agacctgcaa
    10321 aacaaaatcg acagcctgac ggaagaaaat gcacgatacc gcgccctgct cgaacagagc
    10381 agggagaaaa tcagcgcact ggcagcgcgc ctcccccaac ggcaggaaac gcagcaataa
    10441 ggattaaagg atgaacatcg aacaagtcta catcgaagtc atgcacgccc ggctgaccgt
    10501 caacacgccg gcagaagaaa aagacacact gttgcaggca gtcggaatgc tcaacggcaa
    10561 agccgaagcc atccgcgaag gcggccgcgt cgcggacagc gaaaaaatcg tcattatggc
    10621 cgcgctcaac gtcgtccacg acctattgaa aacctccctg aacggcggcg atttggcaat
    10681 cggcgatttt gcgcgtaaaa taaccgatat ggacaacgcc tgccaaaagg cactatcccg
    10741 cttggggcag gaataacccc tttttccctg cggtgtccgc gaaggcatat attcctttga
    10801 accaataagt tcgcttaagg ttgccgggag cagtagtggg cgcgagcgtc cttttgcgga
    10861 cgcacccgaa actacccggg gcagccgcct tgtcagcaag gttcaagcgg attcggccga
    10921 aacggctctc gcggggattc ccattcaaaa ccgcatcgca acgatgcggt ttttcacatt
    10981 ggccgccgcc ccgcccgaaa aagcagtatg cagtctgccg aaatccggaa aatccttccg
    11041 taacgcggta atgcgattga caaaatcaaa tcctgtcttt aaaatccata actttctata
    11101 tctatctgga tttccactat gaaaaccttt tcagcgaaac ctcacgaggt aaagcgcgaa
    11161 tggttcgtca tcgatgccca agacaaagtc ttgggtcgcg ttgcaaccga agtcgccagc
    11221 cgtctgcgtg gcaaacacaa acctgaatac accccccacg tcgataccgg cgattacatc
    11281 atcgtcatca atgcggacaa actgcgtgta accggtgcca aattcgaaga taaaaaatac
    11341 ttccgccatt ccggttttcc aggcggcatc tacgagcgca ctttccgcga aatgcaagat
    11401 caattcccgg gccgcgcttt ggagcaggct gtaaaaggta tgctgcccaa aggtccgctg
    11461 ggttacgcca tgattaaaaa actgaaagtg tacgctggtg cggagcatgc ccatgctgcg
    11521 caacaaccca aagttttgga actgaaataa taaggacacg acatgaacgg taaatactac
    11581 tacggcacag gccgccgcaa aagttcagtg gctcgtgtat tcctgactaa aggtacgggt
    11641 caaatcatcg taaacggccg tcccgttgac gaattcttcg cacgcgaaac cagccgtatg
    11701 gttgtccgcc aacctctggt tctgactgaa aacgccgaat ctctcgacat caaagtaaat
    11761 gttgtcggcg gcggcgaaac cggccagtcc ggcgcaatcc gccacggtat tacccgtgcc
    11821 ctgattgatt ttgacgctgc tttgaaaccc gccttgtctc aagccggttt cgttacccgc
    11881 gatgcgcgcg aagtcgaacg taaaaaaccg ggtctgcgca aagcacgccg tgcaaaacaa
    11941 ttctccaaac gttgattttg gaaattcaaa aaaccctgct tatcgcaggg ttttttattt
    12001 gtaacaggcg gtttcccatt ggtaatctaa agattacgga ttgggcaaaa atcaaaaaca
    12061 gcaccagtat cgactacacg gacgaactga ccgtcaggga tggctgttac tggagaggac
    12121 atcgtatttc tactttgtaa aataactttt aatttattta gagttattga aaaaatagaa
    12181 cggctatttc aactagccta taaaaatggc aatctattag ggttagcata ggtgtcaccg
    12241 ccgccgttca agccgatcag taaaggaaca gtttcctagt ttttagctaa cctgtattat
    12301 ataatgggga gaatatctta aataaccaag catccttaat cataatttag agaaattatt
    12361 ttttcagagt ctaactcttt taatatgatt aacttattaa ctttatctat ttcccagtct
    12421 gatattatat cggtagatac tgaattctct cttattaaat ttttatctgt aataattgca
    12481 cccaactcat gaataataca aattttttga ttatccaata aataaatatc aaaaactatt
    12541 ccatctatat ttagtatgtt aacttttgaa ttttgtatag aaatacaaaa aacgactgaa
    12601 tcaaatccga taaaaatatt tttatcatca agcattaata cttttggggg aatacttatg
    12661 caagaaacag ctaatccaat acctatattg aattctttat aaaaagaaag aataaaacat
    12721 cgataaatct cttttcctcc ctcttgtaga ataatatatt tttgataggt gtttattaaa
    12781 tagtcttcta actctcttat ggagttataa ggatatttcg ttaatctcat tattttgctc
    12841 catgtctaaa acgtcctgtt gatccatata tttcacccgt tatgagtctg ttcccatctc
    12901 atatagtttc aagtttaaca accccttgag gctatgttaa aagagttttt ctaataacta
    12961 ttccgttttc ttaaatataa aaaaatagaa aaatagcccg ggtatagttt gaacaaggga
    13021 aatcagaccg gttccaactc acttaatccg ggcaagtccg cgaagccgcg ttcacgtatg
    13081 atttggcaaa agttgttcag ataactttta tctgtgtctt cagtgcggat ggcggcatac
    13141 agtttgcttt gcagtccgtc ggcagtaatt tggcggtgga cgacatagcc tttttcaagg
    13201 tagggcatga ctgtccaata gggaagggcg gcaatgccac gtctgctggc aaccagttgg
    13261 ataatggcga tggtcagctc gctgtgccgg cgcggcgggt tgatgttttt cggaatcagg
    13321 atttttttgg gtaaatccag catttcatcg ggaacgggat aggtaatcag ggtttccccg
    13381 ataaagtctt ccgccgtcca aacgtttttg gcggcaagcg gatggtccgg ggcgcaaatg
    13441 ccgaccattt cgtaggcaaa cagtgggtgg aagctgatgc tgctttgttt ttcggcttcg
    13501 gagacaatgg cgaggtcggc gcggtgttgc agcagcagcc cgacgggatc cgcttggaat
    13561 cctgatacga tatccagttc gacttggggc cacatcgggc ggaattcgcc catggcgggc
    13621 atcagccagt cgaaacaggt atggcattcg acggcaatcc gcagctctcc cgcctcccct
    13681 tccgtgattc gcgccaaatc tcgttctgca acagcaactt gaggtatcag ttcgtgggcg
    13741 aggcgcagca gcctttcgcc caccggggta aagcgcaggg gcgtggattt gcgttcgaac
    13801 agcggcgtgc cgtagtggtt ttcgagcata cggatctggt gggaaagggc ggattgggta
    13861 aggaaaaccc gtttggcggc aagggagacg ctgccggttt cttcaagtgc cagcagggtt
    13921 ttgaggtggc gcaattcgat aatggaatcc atggggcttc agacggcata ttgaaccggc
    13981 gcatattaaa ataattcata tgcgggtgca aatcggaaaa atgggcgttt cgggcatttg
    14041 cggttagaat gcccgcttgt ttggaaatga caggaaaaat atggttttgc gcagagattt
    14101 tttagcctgg tgcgacgaaa cgttgcagac ggcatcgttt aaagattacg cccccaatgg
    14161 tttgcaggtt gaagggaggg aatatatcgg gaaaatcgtt acgtcggtaa cggcaagcag
    14221 ggcagcgatt gattttgctg tggagcagaa ggcagatttg cttttggtac atcacggtat
    14281 gttctggaaa aacgagctgc cgaccgttac cggttggaaa aaagaacgga ttgccgcact
    14341 gttacggcac gacatcaata tggcaggcta ccatctgccc ctggatgtgc atcccatact
    14401 gggcaacaat gcccaactcg ccgacagatt gggttttgcg acagaaaaac ggttcggcga
    14461 acaaaacctg ctcaactcgg gcagcctgaa acaagccaag acactcggcg cattggcggc
    14521 gcatattgaa acagttttgc aacgtaagcc tgtcgccatc ggcaatcccg aacgcgaaat
    14581 ccgacgggtt gcatggtgta ccggcggtgc acagggcttt tttcagacgg caatagatga
    14641 aggcgtcgat ctgtatctga cgggtgaaat ttccgaagcc caataccacc tcgccaatga
    14701 aacgggtacg gctttcattt cggcagggca tcacgcgacg gaacgttacg gcgtacgcgc
    14761 gctggcagaa tcggcggcag aggttttcgg gttggaagtg tgccattttg acgaaaacaa
    14821 cccggcttga gtcttgagaa atatcataaa actttacctt attttaaata atgctttgag
    14881 tatcactgca aactgggtaa aattgcaatg tttaatggct cggcattccc aaaatattag
    14941 gacgactgaa taatggataa tcaagaaatc aacaacggcc gccgccgttt cctgacactt
    15001 gcgacctgcg gcgcgggcgg agtggcagca ttgggtgtgg caacgccgtt tgtggccagt
    15061 tttttccctt cggaaaaagc caaggccgcc ggtgctgccg tcgaagtgga tgtcagtaaa
    15121 atcgaagcgg gtcagctgct gaccgccgag tggcaaggca aaccgatttg ggtgctcaac
    15181 cgtacagatc agcagcttaa agacctgaaa ggcctgaacg gcgaacttac cgatcccaat
    15241 tccgatgcgg aacagcagcc ggagtatgct aaaaacgaga cccgttcgat taagccgaac
    15301 atccttgtcg ccatcggtat ctgcacccat ttgggctgct cgcccacctt ccgtcccgac
    15361 attgcccccg ccgatttggg cgcagactgg aaaggcggct tcttctgccc ctgccacggt
    15421 tcgaaattcg acttggccgg ccgcgtatat aaaggtgttc ctgccccgac caacctggtt
    15481 gtcccgccat ataaatactt gagcgacaca actatcttgg tgggcgaaga ctaagaataa
    15541 ggaacgataa ttatggcaaa ccaaaccaat agcaaagcaa aagcattgtt aggctggatg
    15601 gacgctcgct tccccctgtc taaaatgtgg aatgagcatt tggcgcaata ctatgcgccg
    15661 aaaaacttta atttttggta ctatttcggc tctttagccc tgctcgtcct cgtgattcaa
    15721 atcgtcagcg gtattttcct gacgatgaac tacaaaccgg acggcaacct gaatgcctac
    15781 catctgcctg ctgcctttac cgcagtagag tacatcatgc gcgacgtgtc cggcggttgg
    15841 attatccgct acatgcactc caccggcgca tctttcttct tcattgttgt ttatctgcac
    15901 atgttccgtg gtttgattta cggttcatat aaaaaaccgc gcgaattggt gtggattttc
    15961 ggttctttga tcttcttggc attgatggcg gaagccttta tgggctacct gctgccttgg
    16021 ggtcagatgt ccttttgggg tgcgcaggtg attatcaacc tgttctccgc cattcccgtc
    16081 atcggtcctg atttgtccac ttggattcgc ggcgacttca acgtttccga cgttaccctg
    16141 aaccgcttct tcgccctgca cgttatcgcc gtaccgctgg tattgctcgg tttagtcgtg
    16201 gcacacatca tcgcactgca cgaagtgggc tctaacaacc ccgacggtgt ggaaatcaag
    16261 aaaaacaaag atgaaaacgg cattccgcgc gacggcatcc cgttccaccc ttactacact
    16321 gttaaagaca tcctgggcgt cgttgtattc ctgattgtct tctgtgccgt attgttcttt
    16381 gcccctgaag gcggcggcta cttcctcgaa gcgccgaact tcgatgcggc aaatgcgctg
    16441 aaaacacccc cgcacattgc gccggtatgg tatttcacac cgttctacgc cattttgcgt
    16501 gcgattcctt ccttcttggg aacgcaggtt tggggtgtaa tcggtatggg cgcggcggtc
    16561 gtgttgattg ccctgctgcc ttggctggac aggggcgagg tgaaatctgt ccgttaccgc
    16621 ggcccgattt tcaaaaccgc attggtcttg ttcatcattg cctttatcgg tttgggtatt
    16681 ttgggcgcga tggtggcaac cgatacgcgt actttggttg cgcgcatcct gtccttcgtc
    16741 tatttcgcat tcttcctggg tatgccgttc tataccaagc tggataaaaa caaaccggtt
    16801 cccgaacgcg taaccatgag caccgccaaa caaaaaatca tgttctttgt ttacgtcggc
    16861 attactgccg tgggtgctta cctgtttgca accaacatct gatgagggca gtcgaaatga
    16921 aacaagctat gaagaattgg tttgctgcct tgctgtcggc agtacccatg agtgcggcat
    16981 ttgcttcggg cggtcatgca cactatgaga aagtcgatat cgatttgcgc gaccaagtaa
    17041 gcttgcagcg cggcgcacaa atctttacca actactgttt gtcctgccac tcggcaagcg
    17101 gtatgcgttt caaccgtttg aaagacatcg gtctgactga cgaagaaatc aagaaaaacc
    17161 tgatgtttac caccgataat gtcggcgatg tcatgcattc ggcgatgaac ccgaaagatg
    17221 cggcaaaatg gtttggtgct gctccgcccg atttgacatt gattgcgcgt tccaaaggtg
    17281 cagactacct ttacgcttat atgcgcggct tctataaaga tccgacccgt ccgagcggct
    17341 ggaacaatac tgtattcgat aaagtcggta tgccccaccc gttgtgggaa cagcaaggcg
    17401 ttcaagccgt tgagttggat gccaaaggtc agccggttat ggtaaaagac gaacacggcg
    17461 agatgaagcc taagctgtat tgggaatcta ccggtttgca cagccgccgt ctgcctaacg
    17521 gcaaagtgat ccagaaagag tacgacgcat atgtacgcga tttggtcaat taccttgtgt
    17581 acatgggcga acctgcacaa ctgcaacgca aacgtatagg ctatgtcgtg atgattttcc
    17641 tatttgcggt tatgctgcct ttggcgtatt tcctgaaaaa agaatattgg aaagacgtac
    17701 actaagcgtt tggaacaaaa gggcaaatcc tttagggttt gccctttttt cattttgcct
    17761 gccgtttgaa aagcctgaat ccgtatgccg tctgaacata gctgcaacat ttcagacggc
    17821 atcattctaa aaatgtcagg catcgggaac ttaatcaggg tttgtggcag attgatattg
    17881 aattaggaaa aggtctgaaa ccgcaagcag catggtaagg cgatgtcgaa caggtttaaa
    17941 gccaatccgc tatatttttt ggagctgcac cgtgcaaaca ggtagaatgg cggcggtttt
    18001 gtccggtgtg aaaatgtttg aatattagga tgattgatgg agctgatgac tgttttgctg
    18061 cctttggcgg cgttggtgtc gggcgtgttg tttacatggt tgctgatgaa gggcaggttt
    18121 cagggcgagt ttgccggttt gaacgcgcaa ttggcggaaa aggcggcaag atgtgatttt
    18181 attgaacagg cacacgcaga aattgcatca gaattggccg ctttggatgg gaaatacgag
    18241 catttgcagg acgaaaatta tgctttgagc aaccgttttt ccgcagccga aaagcagatt
    18301 gcccatttgc aggaaaaaga ggcggaatcg gttcggttga agcagtcgta tatcgatttg
    18361 caggaaaagg cgcagggttt ggcggttgaa aacgaacgtt tggcaacgca gctcgggcag
    18421 gaacggaagg cgtttgccga gcaatatgcg ctggaacgcc aaatccgcca aagagtcgaa
    18481 accgatttgg aagaaagccg ccaaactgtc cgcgacgtgc aaaacgatct ttccgatgtg
    18541 ggcaaccgtt ttgccgccgc cgaaaaacag attgcctatt tgcaggaaaa agaggcggaa
    18601 gcggagcggt tgaggcagtc gcataccgag ttgcaggaaa aggcgcaggg tttggcggtt
    18661 gaaaacgaac gtttggcaac gcaaatcgaa caggaacgcc ttgcttctga agaaaagctg
    18721 tccttgctgg gcgaggcgcg caaaagcttg agcgatcagt ttcaaaatct tgccaacacg
    18781 attttggaag aaaaaagccg ccgttttacc gagcagaacc gcgagcagct ccatcaggtt
    18841 ttgaacccgt tgaacgaacg catccacggt ttcggcgagt tggtcaggca aacttatgat
    18901 aaagaatcgc gcgagcggct gactttggaa aacgaattga aacggctcca agggttgaat
    18961 gcgcagctgc acagcgaggc aaaggccctg accaacgcgc tgaccggcac gcagaacaag
    19021 gttcagggca attggggcga gatgattctg gaaacggttt tggaaaattc cggccttcag
    19081 aaagggcggg aatatgtggt tcaggcggca tccgtccgaa aagaggaaga cggcggcacg
    19141 cgccgcctcc agcccgacgt tttggtcaac ctgcccgaca acaagcagat tgtcattgat
    19201 tccaaggttt cgctgacggc ttatgtgcgc tacacgcagg cggcggatgc ggatgaggcg
    19261 gcacgcgaac tggcggcgta tatcgccagc atacgcgcgc atatgaaagg cttgtcgctg
    19321 aaggattaca ccgatttgga aggtgtgaac acattggatt tcgtctttat gtttatccct
    19381 gtcgaaccgg cctacctgct ggcgttgcag aatgacgcgg gcttgttcca agagtgtttc
    19441 gacaaacgga ttatgctggt cggccccagt acgctgctgg cgactttgcg gacggtggcg
    19501 aatatttggc gcaacgagca gcaaaaccaa aacgcgctgg cgattgcgga cgaaggcggc
    19561 aagctgtacg acaagtttgt cggcttcgta cagacgctcg aaagcgtcgg caaaggcatc
    19621 gatcaggcgc aaaacagctt tcagacggca ttcaagcaac ttgccgaggg acgcgggaat
    19681 ctggtcggac gcgccgagaa actgcgtctg ttgggcgtga aggcaggcaa acagcttcaa
    19741 cgggatttgg tcgagcgtgc caacgaacag acggcatttg ctttgggcaa accttcggaa
    19801 caggaagcgg ctgacgaagc cgaataggaa aggcggatgc gtttcagacg gcatcagtgc
    19861 agatgatgcc gtctgaagac tttgggaagt cggttagaaa aacggattgt gccgtttttc
    19921 gtgtccgatg gaagtcatac gcccgtgtcc ggcgacaact tgcacggttt cggggaaggc
    19981 gaataatttg ttgcggatat tgttgattaa gtcggcgtgg ttgccgcgcg gaaaatcggt
    20041 cctgcctatg gtttcgtaaa acagcacgtc gcccgcaatc agcagctccg cttcggcaca
    20101 atagaaaacc acgtgtccgg gcgtgtgccc cggaatatgc agcacttgga aggcatagcg
    20161 tccgaccgtg agcgtttcgc cttcttcgag ccaacggttc ggcgcaaagg cgggcgagac
    20221 ggggaatccg tattgcgcgg tggtttgcgg cagcgattgg agcaggaatt catcttcgcg
    20281 gtgcgggccg aggacgggga ctttatgcgt ttccagcatc tcgaccacgc cgcccgcgtg
    20341 atcgagatgg ccgtgcgtca gccagattgc cgtgagcgta agtttgcggt ttgccaacgc
    20401 ttgcagcagg aacggcacgt cgccgccgac atcggtcagg acggcttcgc cgctttcgtc
    20461 gtcccaaatc agggtgcagt tttggcggaa gggggtaacg gggagaattt cgtaacgtag
    20521 ggtcatcggc gtgttcctaa aacggttttt cagacggcat cgggtttgcc gtttgtttta
    20581 tggcggtttg ccgcccgttt tgatattggg ggaatcagat gattaagaag acaatcggcg
    20641 gcatcatacc gatttttacg gcggttttca tccctgcatc ggcaggcgcg gcggatttga
    20701 tgctggcgca ggaatacaaa gggcaggaca ttgccggctg ggcgatgagc gagaaactcg
    20761 acggcgtgcg cgcctattgg gacggaaagc acctgataag ccgtcagggc tacgcgttta
    20821 cgccccccaa aggctttacc gcgcagtttc cgccttatcc tttggacggc gaattgtata
    20881 gcggacgtgg tcagttcgag cagatttccg ctaccgtgcg ttctgtttct tcagactggc
    20941 gcggcatccg cctgcacgtt ttcgatgtac ccaaggcgca gggcaacctc taccaacgtt
    21001 tggcagtcgc aacgcagtgg ctgaaaacgc atccgaacgc gccgattacc atcatcccgc
    21061 aaatcaaagt gcgcgaccgg cggcacgcga tggacttttt aaaacaaatc gaagcgcagg
    21121 gcggcgaagg cgtgatgctg cgccagcccg aatcccgtta cagcggcggc aggagcagcc
    21181 aattattgaa gctgaaaagc caatacgacg acgaatgcac ggtaacgcgg cactatgagg
    21241 gcaaagggcg aaacgccgga cggctgggcg cggtcggctg caaaaaccga cacggcgaat
    21301 tccgcatcgg cagcggtttc aaggacaaag accgcgacaa cccgcccaaa atcggcacgc
    21361 tgattaccta ccgttaccgt ggctttacgc ggaaaggcac gccgaaattc gccaccttcg
    21421 tgcgcgcacg taccgaccgt tgagcaggac ggctcggacg gcatccgata ctttggttta
    21481 taattttcct tttaccgacc gattccgaca tatgaccgat ttagaaacca aacgccttga
    21541 aacacaggcg atgcttgaaa acgccgatct tttgttcgac caaggccaat gccgtgccgc
    21601 actgcaaaaa gtggcggacg agattacgcg tgatttgggg gataaatatc cgttgctgct
    21661 gcccgtgatg ggcggcgcgg tggtgtttac ggggcagttg ctgccgctgt tgcgttttcc
    21721 cttagatttt gattatgttc acgtttcccg ttacggcgac aagctggagg gcggcgcgtt
    21781 caactggaag cgtatgcccg atgcggaaca aatccggggc aggcacgtgg tcgtgttgga
    21841 cgatattttg gacgaagggc atacgatgtc cgccattcaa gccaaacttt tggaaatggg
    21901 tgcggcaagc tgccgtgcgg cggtgttcgc caataaatta atcgacaaag aaaaaccggt
    21961 caaagccgat tatgtcggac tggatgtgcc gaaccgttat gttttcggtt acggcatgga
    22021 tgcggcaggc tgctggcgca atttgggcga gatttacgca ttgggcggaa aataagggat
    22081 gcgatgccgt ctgaaggcct ttcagacggc atcgcgccca tacgccggca ggattaatga
    22141 ggaacaggac gcatcaatat gatagggctt ttaatcatca cacacgaaac cataggcgaa
    22201 gcctaccgca agctggcgca tcattttttt ccgggcgggc tgcctgaaaa cgtccgcata
    22261 ctcggcgtgc agccgacgga aggccaagac gacattatca acaacgccat ttccgcgctg
    22321 caagagtttc ctgaaaacca cggtgtattg attatgaccg acatcttcgg cgcgaccccc
    22381 tgcaatgccg cccgccggct cgtgcgcgaa aacaaatcgg cgattttgac cgggctgaac
    22441 gcgccgatga tgattaaggc cgtccaatat tcgccggcgg cggaagacct tgccgccttt
    22501 accgaatgcg tcagggaggc ggcggtaaaa ggcattttcg ccatcacgtc cgcgcccgaa
    22561 gatttggtgt gccggcgcag cggcgatgcc gtctgaagaa gcggcagggc aggaaaacat
    22621 tttaaacggc gtctgctgcc gatatatagc ggattaacaa aaatcaggac aaggcggcgg
    22681 gccgcagaca gtacggatag tacggaaccg gttcgcccgg tgcttcagca ccttggagaa
    22741 tcgttctctt tgagccaagg cggggcaacg ccgtactggt ttttgttaat ccactataca
    22801 taacacggga accgaaatgc tcaaacaatc catcgaaatc atcaacaaac tcggactcca
    22861 cgcccgcgcg tccagcaagt tcacccaaac cgcgtcccaa ttcaaaagcg aagtctgggt
    22921 tacgaaaaac ggcagccgcg tcaacggcaa aagcattatg gggctgatga tgctcgcagc
    22981 cgccaagggt acggtcatcg aactggaaac cgacggcttg gacgaagccg cagccatgaa
    23041 ggcattgacc gacttaatca acgactactt cggcgagggc gaataatgag tatcgtgctg
    23101 cacggcgtgg cggcgggcaa aggcattgcc gtcggttgcg cccacctgat tgcgcgcggt
    23161 acggaggaag tgccgcagta tgatgttgct caggcggaca ccgatgccga agccgaacgt
    23221 ttcgatgccg ccgtcaaagc cacgcgcaaa gagttggaac agctccgcag cgcgattccc
    23281 gaaaacgccc cgaccgagtt gggcgcgttc atctcgctgc acctgatgct cttgaccgat
    23341 gttaccttgt cgcgcgaacc cgtcgatatt ttaagggaac aaaaaatcaa cgccgagtgg
    23401 gcattgaagc agcagagcga caaactcgcc gcccaattcg acaatatgga cgatgcctat
    23461 ttgcgcgaac gcaagcagga tatgctgcaa gtcgtccgcc gcatccacaa caacctgatc
    23521 gggcagggca acgagttgga ggttgccgac aacctgtttg acgaaaccgt tctgattgca
    23581 aacgaccttt cacccgccga cacggttttg tttaaagagc agcgcattgc cgccttcgtt
    23641 accgatgccg gcggccccac cgggcatacg gcgattttgg gcaggagctt ggacatcccg
    23701 tccgtcgtcg ggctgcacaa cgcgcgcaaa ctgattaccg aaggcgaaac ggtcatcgtg
    23761 gacggcatca acggcgtgtt gattatttcg ccggatgagt cggtgttgaa cgaataccgc
    23821 cgccgcgccc gcgaataccg cagccacaaa cgcgatttga acaagctcaa aaaaaccgcc
    23881 gccgccaccg ccgacggggt ctgcatcgag cttgtgggca atatagaatc cgccgaagac
    23941 gtgaaaccgc tgcacaacct cggcgcagac ggcatcgggc tgttccgcag cgagtttctt
    24001 tacctgaacc gcgatacgat gccgtctgaa gacgagcagt acgaagtgta cagcgcgatt
    24061 gtcaaaaaaa tgaagggcaa aagcgtaacg atacggacag tcgatttggg tgtggacaaa
    24121 aatccgcgct ggttcgggaa aaacagcacg cccaacggca gcctcaaccc cgcgctgggc
    24181 atgaccggca tccgcctgtg ccttgccgaa ccggtcatgt tccgcacgca gatgcgcgcc
    24241 atcctccgtg cggcggtaca cggccccgtc cgcatgatgt ggccgatgat tacctccgta
    24301 tccgaagtgc gccagtgcct catccacctc gacaccgcgc aacgccagct tgccgaacgc
    24361 ggcgatgcct tcggcgaagt cggcatcggc tgcatgattg aaattccgtc tgccgcgctg
    24421 accgtcggca gtattttgaa actggtcgat ttcatctccg tcggtaccaa cgatttgatc
    24481 caatatattc tgtccgtcga tcgcggcgac gacagcgtca gccatctcta ccaacccgga
    24541 catccttccg tgctgaaaat gctgcaacac gtcatccgta ccgccaaccg catggacaaa
    24601 gacgtgtccg tatgcggcga gatggcgggc gataccgcgt ttacccgcgt tttattgggt
    24661 atggggctgc gccgtttttc catgaatccg aacaacatcc tgcccgtcaa aaacatcatt
    24721 ctgcacagca atgccggaca gctcgaaggc gatattgcga aagtcatccg ctgcgaagac
    24781 gaagagaagt cggaaaagct gatcaaacag atcaacagcg tgtctgtcgc ggaagaagcc
    24841 gaagtcaagg gacggaaata aatattgttg acaatgcgag cgggttgcag tcaaataacg
    24901 cccttaaaaa atacagctgc tttggtcaag aaatgccgtc tgaaaaattt tcagacggca
    24961 ttttgtttgg caggcatttc tgccgcatca tcggaaacgg acgatacgcc cttcatcgac
    25021 gcggatgcgg accgtaccgt ttccggaaat accgtccgta tgttgtccga cagtcaggtt
    25081 taaggtcagc tcgccgtgtt cgggatggac ggcggaaagc cggagcgagt cgggcaggcg
    25141 gacgagggac agcaggcggc attccgttcc atgattgtcc aagcacacgg cattttgcgg
    25201 aatatggcgg tcatcgtcgg tattgggcag ccccatcaga cgggcgacct gcacgccggc
    25261 aggcgtttga atcaaggttt cgggcgtacc gcattgaagg attttcccct cgtgcatgac
    25321 ggcgatttcg tccgccgccg tgcaggcctc ttcgggcgaa tgcgttacca aaacggcagg
    25381 gatgccgccc ttgcggatgc gttcggcggt catacggcgc agccggtcgc gcaaatgcgt
    25441 gtccaaactg gaaaacgatt catccagcaa cagcagggaa gggcggacaa ccaaagcgcg
    25501 cgccagtgcc aaccgttgct tctcgcctcc ggaaagtttt tcaggcttgc ggtgcgcctc
    25561 gttttccagc ccgacttcgg caagtgccga caaggcgagg cgttcggctt cggctttcgg
    25621 cattttttgc attttcaaac cgaatgccgt attttccagc gcactcatat ggggaaacag
    25681 cgcgtaatct tgaaacatca gcgagatacg gcgtttttcg ggcggcatac aggtaatgtt
    25741 ttccccgttc agccgaattt cgccgccgtc cggccggacg atgcccgcaa tcatattcag
    25801 cagggtggat ttgccgcagc ccgaccgccc cagtacggcg agtattttgc cgcgcccgac
    25861 agtcaggcag atgttgtcgg cgaccgtttt gccgccgaag catttgcaga gtccgttcag
    25921 ttcaagcatg gcgcatccta taaacgtatg ccgtccagcc actcggacag cggatgggcg
    25981 gcatcaatca gaccgtaacg gtaaaacgcg tccagcgtaa ccagttgcgc gtcgtgcatc
    26041 atgtttttcg acaacatggc atccaatagg ccgccaatgt ccatcttttc aaaacccgct
    26101 acctcgccat cctgattttc aggcaggaag gtttcgggca ggacggcatc gaacacatac
    26161 aggatttcat tgtgcacacc tcggctgacg gggcgaaggc tgtgcagccg cgatactggg
    26221 cggatgagcg gaaacagcgt tttatccaaa ccggcttctt cgctgctttc gcgacacacg
    26281 gcttcagacg gcatttcgcc gccggaaaca ccgccgccgg caatattgtc gagcttgccg
    26341 ggatcgactg ctttgtgcgg actgcgcctg cctatccaaa aatgccatct gccgttcgat
    26401 tcgaccaaac cgttgagatg gacggcgcgg ctgagtagtc cgaacggacg gaaagcggcg
    26461 cgttcgagcg tgaacaaggg gttgccgccg ccgtcggtca ggtcgaaaca ctcgttgcgc
    26521 catccgtgaa gcagccccgc cttgttccat gtgcgggcga ggtgctgcaa gcgtccgccc
    26581 atatccggcc agccgtccgc attcagaaaa atgccgtctg aagactcgga gcagcctgcc
    26641 tcccagtctt ttttgatgcg ctcagcccat tccggcgaca gattgcccaa aggaagacgg
    26701 ttcagataca gcgttttcca gcaactttcg gcaccgtaac ttgcttttgc ccgctcgaac
    26761 agggcatcaa ggtcttgttt gctgacggat tcggtaaaac ggacggtcgg catagccggc
    26821 tttcttgaac acaatgcgga agattgtagc caaaccgcct gaatttgcaa ccgcaaacag
    26881 acggcggcct gccgttccga tattgcggca ggaaggaaaa tccggcaaaa aacggaggcg
    26941 gcaggaaaca ggaaaaacaa aaccccctcg gcattgaacc gaggggtttg atatttggtt
    27001 gcgggagcag gattcgaacc tacgaccttc gggttatgag cccgacgagc taccatgctg
    27061 ctccatcccg cgtcagaaga tgaaactata cggcagattt tttatgttgt caaacattat
    27121 ttccgtgaaa aatcataaag ttttccgtcc ggcggcttat gcctgatttg aaatatcatt
    27181 ttctttacaa aagttcatgt tcgtgattta attttggtta acatcgaaac aggggtgctg
    27241 cctgatgttc aggcggctga gaaataccct ttacacccga tcgggataat acctgcgtgg
    27301 ggagttttca cggattctgt ctttcagacg gcattggttt tcaaatgccg tctgaaaacg
    27361 caaaacgctc ctgtttcttt attctaaacg agaaaacagg agcatttttt atgactacgc
    27421 caaaaaaaac cgccaaaatt tccggcaacg aagcgcgcga actttccgac ttgagcgaag
    27481 acatcggcat ccgcttcaaa tatccgaact ccgaacgcgt gtatctgcaa ggcagccgcg
    27541 acgacatccg cgtgcctttg cgcgaaatcc gtcaggacga cacctacacg gcgcaaggta
    27601 cggaagccaa cccgcctatt ccggtgtacg acaccagcgg cgcatacggc gatccggcgg
    27661 cgcacatcga cctgaaacaa ggcctgccgc acatccgcac ggcgtggctg gacgaacgcg
    27721 gcgataccga aatcctgccc aaactctcca gcgaatacgg catcgaatgc gcacacgatc
    27781 cgaaaaccgc ccatctgcgc ttcaaccaaa tcacccgccc gcgccgtgcc aaagccggcc
    27841 gcaatgtaac ccagcttcac tacgcgcgcc aaggcattat cacgcccgaa atggagttcg
    27901 ccgccatacg cgaacgcatg aagctggacg agcttttcag acggcctgaa tacgccaagc
    27961 tcttgaaaca gcacacaggg caaagtttcg gtgcgaatat cccgacccgt cccgaccaaa
    28021 tcacccccga attcgtgcgc caagaaatcg ccgccggacg cgcgattatc cccgccaaca
    28081 tcaaccaccc cgaactcgaa ccgatgatta tcggccgcaa cttccgcgtc aaaatcaacg
    28141 gcaacttggg caattccgcc gtaacctcca gcctgaccga agaagtcgaa aaaatggtgt
    28201 ggtcgctgcg ttggggcgcg gacacgatta tggacttatc cacaggcgcg cacatccacg
    28261 aaacgcgcga atggattatc cgcaacgcgc ccgtccccat cggcacagtg ccgatttacc
    28321 aaactttgga aaaaaccggc ggcatcgccg aagatttgac ttgggatttg ttccgcgaca
    28381 ccttaatcga gcaggcggaa caaggcgtgg actatttcac catacacgcg ggcgtgttgc
    28441 tgcgttatgt gccgatgacc gccaaccgcc tcaccggcat cgtatcgcgc ggcggttcga
    28501 ttatggcgaa atggtgtttg gcacatcatc gggaaaactt cctctacacg catttcgacg
    28561 aaatctgcga aatcatgaag gcgtatgacg tatcgttcag cttgggcgac ggcctgcgcc
    28621 ccggctgcat tgctgatgcc aacgacgaat cccaattcgc cgaattgcac accttgggcg
    28681 aattgaccga taaagcgtgg aaacacgacg tacaagtcat gatcgaaggc cccggccatg
    28741 tgccgctgca acgcgtcaaa gaaaatatga ccgaagagtt gcaacactgc tttgaagcac
    28801 cgttttacac actcggcccg ctcgttaccg acatcgcccc cggctacgac cacatcactt
    28861 cgggcatagg cgcggccaat atcggctggt acggcacggc gatgctttgt tacgttaccc
    28921 ccaaagaaca tttggggctg cccgacaaag aagacgtgcg caccggcatc atcacctaca
    28981 aactcgccgc ccacgccgcc gacctcgcca aaggctggcc gggcgcgcaa ttacgcgaca
    29041 acgccctgag caaggcgcgt ttcgaattcc gctggcgcga ccaattccgc ttaagcctcg
    29101 accccgaacg cgccgagagc ttccacgacg aaaccctgcc cgccgaaggc gcgaaaatcg
    29161 cccatttttg ctcaatgtgc ggccccaaat tttgctcgat gaaaatcacg caggaagtgc
    29221 gcgactacgc cgacaagcaa aaagcccaac ggcagggtat ggaggaaaaa gcggtcgagt
    29281 tcgtcaaaaa aggcgcgaag atttacagtt gaaacgtcaa gcaaaaaatg ccgtctgaag
    29341 cccggaaaaa aggcttcaga cggcattttt tcgcttgtga aaatgattaa acaaccgatc
    29401 attaaagtaa tttttaaaat ttttatattc acatagatgg gaatgatggg atttagggtt
    29461 ctgattttgt tttgagagaa tgaaggaatt tgagattgtg ggcgattatc gggaaaaata
    29521 gaatctttcc gccgtcattc ccgcgcaggc gggaatcccg accgatcggc gcagaaactt
    29581 atcgggtaaa acggtttctt tagattttgc gttccggatt cccacttttg tgggaatgac
    29641 ggtttaggta tttttataga aagccgtagg tggtgtttcg tgcaaacggc agatgctgcg
    29701 tcgcggcagg ttgacggcag gtgcttggtg tcgattttgt tgttgccgtc ggctttgacg
    29761 acgtcgtcgt tgtctttggc ggtgcgcgta accggctgtc cgcagaacca ttttaccgaa
    29821 ccgtcttcac gcctggccca cagggagagt tttttgcctt ggatttcttt gtttacgccg
    29881 gttgaagcca tttgggcggt aacgacgccg tttttgactt caacttcctt aacatatttg
    29941 cctttgattt cggcggggga tgccacgccg gcagaagtgt tgtctttcgg ccatttgccg
    30001 tgattcgggc aatacccggc aacggctgat tttttggaag tgtttgaaat cggcgcgtgg
    30061 tgtttcgtgc aaacggcaga tgattcatcg cggcaggttg acggcaggtg cttggtgttg
    30121 attttgtcgg tgtctttgcc gtcggctttg acgtcgtcgg tgccggcttt ggcgtcgcgc
    30181 ttaaccggct gtccgcagaa ccattttacc gaaccgtttt cacgcctgcc ccacagggag
    30241 agttttttgc ctttgatttc attgtttacg ccgcttggat tcatttgggc ggtaacgacg
    30301 ccttttgcga ccgtaacgct tttaacatat ttgcctttga tttcggcggg ggatgccacg
    30361 ccggcagaag tgttgtcttt cggccatttg ccgtgattcg ggcaataccc ggtaacggct
    30421 gattttttgg aagtgtttga aatcggcgcg tggtgtttcg tgcaaacggc agatgattta
    30481 tcgcggcagg ttgacggcag gtgcttggtg ttgatgttgt cggtgtcggg ggtggcggcg
    30541 gtaacgtcgt cgttggcgtc gttgttgttg gcgccgcgcg taaccggctg tccgcagaac
    30601 cattttaccg aaccgtcttc acgcctgccc cacagggaga gtcttttgcc ttggatttct
    30661 ttgtttacgc cggttgaagc catttgggcg gtaacgacgc ctttttcgac tttaacttcc
    30721 ttaacatatt tgcctatgat tgatgaagaa gatgccacgc cggccttgtc gttgtcttcc
    30781 ggccatttgc cgtgattcag gtaatactcg gtgacggctg atttttgacc ttcggccaaa
    30841 aggatggctt cgtcattccc gcgcaggcgg gaatccagcc tcgtcggtac ggaaacttat
    30901 cgggtaaaac ggtttcttta gattttgcgt tccggattcc c
//
LOCUS       DAXFHF010000022        28178 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXFHF010000022 DAXFHF010000000
VERSION     DAXFHF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28178
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.022"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..173
                     /locus_tag="ACFIRM_000892"
     CDS             <1..173
                     /locus_tag="ACFIRM_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC3028553.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFIRM_000893"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFIRM_000893"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFIRM_000894"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFIRM_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFIRM_000895"
     CDS             2263..2631
                     /locus_tag="ACFIRM_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028554.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHGPYDKDEWIEKIFCL"
     gene            2638..3111
                     /gene="cas4"
                     /locus_tag="ACFIRM_000896"
                     /pseudo
     CDS             2638..3111
                     /gene="cas4"
                     /locus_tag="ACFIRM_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            3289..3474
                     /locus_tag="ACFIRM_000897"
                     /pseudo
     CDS             3289..3474
                     /locus_tag="ACFIRM_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(3514..5442)
                     /gene="rpoD"
                     /locus_tag="ACFIRM_000898"
     CDS             complement(3514..5442)
                     /gene="rpoD"
                     /locus_tag="ACFIRM_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC3028555.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(5629..7401)
                     /gene="dnaG"
                     /locus_tag="ACFIRM_000899"
     CDS             complement(5629..7401)
                     /gene="dnaG"
                     /locus_tag="ACFIRM_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC3028556.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(7554..10304)
                     /gene="secA"
                     /locus_tag="ACFIRM_000900"
     CDS             complement(7554..10304)
                     /gene="secA"
                     /locus_tag="ACFIRM_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC3028557.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            10436..10858
                     /locus_tag="ACFIRM_000901"
     CDS             10436..10858
                     /locus_tag="ACFIRM_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC3028558.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            11141..11692
                     /gene="azu"
                     /locus_tag="ACFIRM_000902"
     CDS             11141..11692
                     /gene="azu"
                     /locus_tag="ACFIRM_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC3028559.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            11787..12296
                     /locus_tag="ACFIRM_000903"
     CDS             11787..12296
                     /locus_tag="ACFIRM_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3028560.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            12330..12950
                     /locus_tag="ACFIRM_000904"
     CDS             12330..12950
                     /locus_tag="ACFIRM_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFC3028561.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            13063..14208
                     /gene="dapE"
                     /locus_tag="ACFIRM_000905"
     CDS             13063..14208
                     /gene="dapE"
                     /locus_tag="ACFIRM_000905"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC3028562.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            14628..15443
                     /locus_tag="ACFIRM_000906"
     CDS             14628..15443
                     /locus_tag="ACFIRM_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3028563.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(15421..15978)
                     /locus_tag="ACFIRM_000907"
                     /pseudo
     CDS             complement(15421..15978)
                     /locus_tag="ACFIRM_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            16328..17080
                     /locus_tag="ACFIRM_000908"
     CDS             16328..17080
                     /locus_tag="ACFIRM_000908"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC3028564.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFKGHLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            17271..17346
                     /locus_tag="ACFIRM_000909"
     tRNA            17271..17346
                     /locus_tag="ACFIRM_000909"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17304..17306,aa:Asn,seq:gtt)
     gene            17477..18487
                     /gene="waaF"
                     /locus_tag="ACFIRM_000910"
     CDS             17477..18487
                     /gene="waaF"
                     /locus_tag="ACFIRM_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC3028565.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSRFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            18542..18988
                     /gene="smpB"
                     /locus_tag="ACFIRM_000911"
     CDS             18542..18988
                     /gene="smpB"
                     /locus_tag="ACFIRM_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC3028566.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            19360..19515
                     /locus_tag="ACFIRM_000912"
     CDS             19360..19515
                     /locus_tag="ACFIRM_000912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028567.1"
                     /translation="MKLEASKQKFKKSFIISLFFSILYTSPLLAVDYVYDNSKLTNEQ
                     IDRLKKT"
     gene            20048..20632
                     /locus_tag="ACFIRM_000913"
     CDS             20048..20632
                     /locus_tag="ACFIRM_000913"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3028568.1"
                     /translation="MGALELTNSLWEPRWNSNIDYLITKNAEIRFNTKNESLLVKEDY
                     AGGARFRFAYDLKDKVPEIPVLTFEKNITGTSDIIFEGKALDNLKHLDGHQIVKVNDT
                     ADKDAFRLSSKYRKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLND
                     KNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKQHL"
     gene            20650..21390
                     /locus_tag="ACFIRM_000914"
     CDS             20650..21390
                     /locus_tag="ACFIRM_000914"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3028569.1"
                     /translation="MQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFRNPDTDNLTTGN
                     VKGFGAGVYATWHQLQDKQTGAYVDSWMQYQRFRHRINTEYATERFTSKGITASIEAG
                     YNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAQVNLLGSRQLQSRVGVQ
                     AKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEIDGDRRVINNKTVIETQLGVAAKIKSH
                     LTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(21373..21678)
                     /locus_tag="ACFIRM_000915"
     CDS             complement(21373..21678)
                     /locus_tag="ACFIRM_000915"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028570.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(21719..23086)
                     /locus_tag="ACFIRM_000916"
     CDS             complement(21719..23086)
                     /locus_tag="ACFIRM_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC3028571.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(23196..23492)
                     /locus_tag="ACFIRM_000917"
     CDS             complement(23196..23492)
                     /locus_tag="ACFIRM_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028572.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEAPAAEAPAAEAPAAEAPAAEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
     gene            23965..24447
                     /locus_tag="ACFIRM_000918"
     CDS             23965..24447
                     /locus_tag="ACFIRM_000918"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3028573.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            24534..25346
                     /gene="hpnC"
                     /locus_tag="ACFIRM_000919"
     CDS             24534..25346
                     /gene="hpnC"
                     /locus_tag="ACFIRM_000919"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC3028574.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(25419..25889)
                     /locus_tag="ACFIRM_000920"
     CDS             complement(25419..25889)
                     /locus_tag="ACFIRM_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC3028575.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            25980..27821
                     /locus_tag="ACFIRM_000921"
     CDS             25980..27821
                     /locus_tag="ACFIRM_000921"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC3028576.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FAPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         6571 a         6648 c         7824 g         7135 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcgacga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catggtccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcggac
     2821 gagccggaaa cgcgcaaggg cgtacgtttt gagcggacgg tacacgtttc ggtagaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata caaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggct tgtgcttgga agaaatgacg gggcaaaccg tctctgaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg tgtccccgtc gtgttttcag acggcctgag acctttgcaa
     3121 taacataggt tactaaaatt ttatgctcaa tctcattttc aaaatgcaaa acttttctga
     3181 tttttcctac tttttgctca atattaggaa ggttttaggc aattgaaaat tttttggcgc
     3241 atttttatgc gtcaaatttc gttaacagac tatttttgca aaggtctcgg cctacgcgcc
     3301 caaaccctcg caactatagc cgccgtccgc gaactcttaa acagcggaca aaccccgccg
     3361 cccgactacg gcaaacgctg caaagcctgc tctctggtgg aaatctgtca gccggagttg
     3421 ttggcgaaac gggatgggag tgtgggatat gtggaagcgt tgtttattgt gtaagaaaaa
     3481 accgcaggta tttgaaacct gcggtttttt ggtttacagc ttgctgtctt cgctgtccaa
     3541 gaaacttctc aaacggtcgc ttcttgtcgg atgccgcagc ttgcggagtg cttttgcctc
     3601 gatttgacgg atgcgttcgc gcgttacgtc aaactgtctg ccgacttctt ccagcgtgtg
     3661 gtcggtgttc atatcgatgc cgaaacgcat acgcaggact tttgcctcac gcggtgtcag
     3721 gctttcgagg atttctttgg ttacttcgtg caggctggtg tacattgccg catcggccgg
     3781 cgcaacattg ttggcatctt cgatgaagtc gcccaagtgc gaatcgtcgt cgtcgccgat
     3841 gggggtttcc atcgaaatcg gctctttggc gattttcatg attttgcgga ttttgtcttc
     3901 gggcatctgc atcagttcgg caagtttggc ggaatcgggt tcttcgccgg tttcttgaag
     3961 gtgttggcgc gagatgcggt tcatcttgtt gatggtttca atcatatgta ccggaatacg
     4021 gatggtacgc gcctgatcgg caatcgagcg ggtaattgcc tggcggatcc accaggttgc
     4081 gtaggtggag aatttgtagc cccggcggta ttcgaacttg tcgaccgcct tcatcaggcc
     4141 gatgttgcct tcctgaatca aatcaaggaa ctgcaagcca cggttggtgt attttttggc
     4201 aatggaaatg acgaggcgca agtttgcctg aatcatttcc tgtttggcgg ctgcggtttc
     4261 tttttcgctc gacaccatat ttttgttgat ttctttcaac tcttcgatgg aaatgcgggt
     4321 ttctttttcc atatccgcca actcggtttg tttttcgagg atggcgtggc ggaagcggtc
     4381 gagtgcgttg ctccaaaccc tgcctttggc gatttcttct tcaatccatt gcagattggt
     4441 gatttcgggc aggaagtttt ggatgaagta gtcgcgttcc atatggacgc ggtcgaggca
     4501 gatgtcgcgg atttcgcgtt cgagtttgcg gatgttttct acttttccgc gcaggctgct
     4561 gctgaggctg tcgatttgcc gggtggcgaa acggacttcc agcagtttgt tggcaatcgc
     4621 gtcgcggtag gcgagatagt ctttgtgccg gctgtggtgt ttttccaaac agccgatcat
     4681 ttttttgtag tctttttcga tttgggcaaa gtggccgatg actttttgtt tcaattcggc
     4741 gagattggct gccgagactt catccgcatc ttcttccgat tcttcgtcgt cttcgttttc
     4801 gtccgaattg tcgttggaag gtttctcggg tgctgtggtt tccaagtgcc ccaagcccaa
     4861 ttcgttgagc aatacttcat tcgggtcgat aatggcttct acgacttcgt cgacgcggat
     4921 ttcgtctttg cagatttttt cgatgagttc taagatttcc gcaatggaac ccgggcaggc
     4981 ggagatggcc tgaaccatat ttttcagggc gttttcgatt tttttggcga tgatgatttc
     5041 gtcttcgcgg gtcagcaggt cgacctgtcc catttcgcgc atatacatac ggacggggtc
     5101 ggtcgtcctg ccgaactcgg aatctgcgct ggaaagggcg gcctcggctt cttcgacggc
     5161 atcatcgtcg gtaacggcgg cattgtcgct taacaatatg tcttccgcat caggggcgtg
     5221 ttcggtaact tggataccca aaccggaaat catactgacg atgttgtcga tttgatcggc
     5281 atcggacata tcgtcgggca gtgcgtcgtt gatttcggag taggtgatgt agccgcgttc
     5341 tttgcccata atgatgagtt ggcgcaggcg cgcacgttgt tcctcaatcg tcagggggcg
     5401 ggcatcgtct tggtattctt cgtgattttg gtttttggac attgtctgct ttctgttggc
     5461 aagatgccga cggtttgcgt ttggacgcac ctgaaggcgt ggcaaagtag ggtactctga
     5521 tgccgtctga aggctttttg ggatgggtgt tctgcaggtg tcgttgttgc agccggggtg
     5581 aatgcttttc agacggcata acggtttaca gatggcggaa tccgccggtc aattttgttt
     5641 tgcggtcagc agcgacagca aaagtttttt ctcgctttca tttaagccgg attgcaggct
     5701 tttttgtttt aatgtttcaa tttggctgta ttttaactca ttgagcagtt ttttgatgcc
     5761 gatttggaag ttttcgcaat cttcttcgcc gccgccttcc atttcttccg attgaagggc
     5821 cgaccggaag atgcggttga ttgtttcttc gtagggcgaa ccgcgcatat gttccaaaac
     5881 ctgtgcggtt gccggcacgg aaggatggtt tttaatggtt tcggcgaggt tggcaaggca
     5941 ggcgaaatcg ccgtccaacg ccagataatc gggcaggtct atatatgcag cccaatccgg
     6001 atttatcaag aggctgcgga tttgccgttg caccaatgtc ggcataacgg gctgtttgac
     6061 ggaaatcgga ggcagtttgt agtttttttg tttgacgtgc cgctttggcg cttcctgtcc
     6121 gagcagttgc gcgaggttgt cggggtcgat gccgaccagc tcgctaagcc gttgttttaa
     6181 caaataagcc aatgccggtg cggtaatctg caccaaaagc ggcgaactgg ttttcaccaa
     6241 ttccgccttg ccttcctgcg tattgagatg aatgccgtct gaaaggtgtt cccagaaata
     6301 ttccgacaaa ggcttgcttt gattcagaag cgcgtcttca aattgcgctt tgccgtaggc
     6361 gcggatgtag ctgtcggggt cgtgttcttc cggcaggaat aaaaaatgca gcgatttgtc
     6421 gtctttcaac tgcggcagcg cgttttccag cgcgcgccaa gccgcttttc gccccgcgct
     6481 gtcgccgtcg aaacagaaat aaatactgtc cgcctgccgc atcaggattt tgacgtgttc
     6541 cgccgtcgtc gccgtgccca aggccgccac accgtagccc acgccgaact gtgccagcgc
     6601 gaccacgtcc atatagcctt cgaccaccaa aatccgttct gcctctttta cagcagcacg
     6661 cccctcatac aagccgtaaa ggtttttccc cttatcgaac aaaggcgtat cgggcgaatt
     6721 caaatatttc ggtttcgagt cgtccagcac cctgccgccg aaaccgatga cctgcccgcg
     6781 cggattgcgg atggggaaca taatccgatg gcggaagcgg tcgtaatgcc gcccctcatt
     6841 gtcaatcacc atccccgtat ccaccaacgc ggtattcgga tacggttgga acacttgcgc
     6901 caaaggctgc cagccgtcgg gcgcgtagcc caaaccataa tgcgcgatga cttccgcgct
     6961 caagccgcgt ttgtccaaat aagctttcgc agccggattg aatttcagct gctgcgcgta
     7021 aaaatcagct gccgccgccg tcgtttcttc cagcgtctgc tgttttttct tgcgctcggc
     7081 acggacttcg ggattatcgt tctgcccgcg cactttaggc acaatcatac ctacgcggtc
     7141 ggcaaggaac tgaaccgcct ccggaaacga cagtccctga tgttccatca caaaaccaat
     7201 cgccgaacca tgcgccccgc aactgaaaca atgataaaac tgcttggtcg gactgaccga
     7261 aaacgacggt gttttttcct tgtggaacgg gcaacacgcc atatagttcg ccccgccttt
     7321 tttcagcgga acctgctcgt cgatgatgcc gacaatatcg gttttggcca gaagctcgtc
     7381 aatgaagtcg gatggaatca tgatgtgggg ggacgggatg tctgttttcg gtacggacgg
     7441 tacgaaatcg ggatttttcg gaatggcggt cggatgtggc ggttcaggca aaatgccgtc
     7501 tgaagccgga aaccggatgt tcagacggca ttacggcatt tgcgttcaaa cgcttaagcc
     7561 aatttgccgt ggcattgttt gtatttcaaa ccgctgccgc aggggcaggg gtcgttgcgg
     7621 tggacgattt gtccccgcgc ttccaaggct tcggggctga aatccgtccc atcgggatta
     7681 aaggcttcgg taaccagatc ggtttgcgac tgacccaaaa gttcttctat atcgggcgat
     7741 tcggaatgga tggactggat gttgccgacg ggttgctctt caaccgccgc gacagggttt
     7801 tgttcgattt gaaccgaagt aagcagggag gcgatatgga atttgatgcc gttccacagg
     7861 tcttggaaca tggtaaaggc ttcgcgttta tattcctgct tcggattttt ctgggcatag
     7921 ctgcgcaggt gtatgccttg tcgcaggtag tccatggcgg caagatgttc gcgccattgg
     7981 ttgtcgatgg cctgcaacat cacgttgcgc tcgaaatcag ccattgcctg cttgccgacc
     8041 agttcggttt tggcggcata ttcgttttcg atacgttcga tcaggcgctc tttgatgtct
     8101 tgaccgtcaa tcgcattgtc cgccttcagc caggattgga tgtcttcctg aagcctgaat
     8161 tcggcagcca ggcggttttc cagtgtcggg atgtcccatt gttcttccat gctgtcgggc
     8221 ggcatatagg tatccacgag gtcgctgacg gcatcagaac ggatttcctg catcaggtcg
     8281 ccgatgtctt tgctggtcag aatttcgttg cgctggctgt aaatgacttt gcgctgttcg
     8341 ttggcaacgt cgtcgtattc caaaacctgt ttgcgcatat cgaagtttct gccttcgact
     8401 ttgcgttgcg ccccttcgat ttggcgcgtc agcaggttgt gctcgatggc aacgccgcgt
     8461 tcgggggcga ggcggttgag gatggcggcg gcgcggtcga gtgcgaagag gcgcaacagc
     8521 gggtcttcaa acgagaggta gaaacggctg gatccggggt cgccctgacg gccggaacgt
     8581 ccgcgcaact ggttgtcgat acggcggctt tcgtggcgtt ccgtaccgat gatgtgcaaa
     8641 ccgcctgctt ccatcacttt atcgtgttct gcctgccagc cgttttcgag tgcggcgatt
     8701 tgtgcctgtt tctcttcgtc gctcaaggtt tcgtcggcgc ggatggcatc ggtttggtgc
     8761 ttcaggttgc cgcctaaaac gatgtccgta ccgcgtcccg ccatattggt ggcgacggta
     8821 attgcgccga ctttgccggc ttgggcgaca atcagggctt cgcgttcgtg ttctttggcg
     8881 ttgaggacgt tgtgcggcag tccggctttt tgcagaaggc gggagaccag ttcggagttt
     8941 tcgatgctgg tcgtaccgac gaggacgggc tgcccgcgtt tgtggcattc ctcgatgtct
     9001 ttgacgacgg cttcgaattt ttcttcggcg gaacggaaaa tctggtcgtt gaagtctttg
     9061 cgctgtacgg ggcggttggt cgggatgatg acggtttcga ggttgtagat gctttggaac
     9121 tcgaaagcct cggtatctgc cgtaccggtc ataccggaga gcttggtgta caggcggaaa
     9181 tagttttgga aggtgatgga ggcgagggtt tggttttcgc gtttgatttc cacgccttct
     9241 ttggcttcta cggcttgatg caggccttcg gaccagcggc ggccggacat cagacggcct
     9301 gtaaattcgt caacgatgac gatttcgccg tcttggataa cgtaatgttg gtctttgtgg
     9361 aacagcgagt gcgcgcgtaa tgctgccata aggtggtgca tcagggagat atttgccgcc
     9421 gagtacagcg agtcgttttc tgccagcagc cccatttggg tcaggatttg ttcggcgtgt
     9481 tcgtgacctg tttcgctcag gatgacctga tgtgcctttt cgtcgaccca atagtcgcct
     9541 tcgccttctt ctgtctcttg acggacgagg tggggcggaa cggtgttcat gatttggtag
     9601 agctggatgt tgtcgtctgc ctgaccggag ataatcagcg gggtgcgcgc ttcgtcaatc
     9661 aagatggagt ccacttcatc gacaacggca aaattcaatt cgcgctgcac tttgtcgtat
     9721 tggtcggtta ccatattgtc gcgcaggtag tcgaagccga attcgttgtt cgtgccgtag
     9781 gtaatgtcgg cagcgtaggc gttttgacgg tcgaacggct gcatatctga aataatcacg
     9841 ccgacggtca gaccgaggaa attgtagagc ggctccataa tgcctgcgtc gcgcgaggcg
     9901 aggtagtcgt tgacggtaac gacgtgcacg cctttgccgg ccagcgcgtt gagatagacg
     9961 gcgagggtgg cgaccagggt tttgccttcg ccggtacgca tttcggcgat tttgccgtcg
    10021 tgcagcacca taccgccgat aagctgcacg tcgaagtggc gcataccgag tacgcggcgg
    10081 gacgcttcgc ggcagacggc gaaggcttcg ggcaaaatgc cgtccaaagt ctgaccgtcg
    10141 gcgaggcgtt gtttgaattc ggcagttttg gcttgcagat cagcatcgct tagggcttgc
    10201 atctgttctt cgagcgcgtt gattctggca acggatttac ggtattgttt cagcaagcgg
    10261 tcgttgcggc tgccgaagat tttcttggca atgtttgtca gcatgaataa ttcctgcgcc
    10321 tgatataata gtggggcgaa aacgcgattt taacacaagc gtttgtgcaa agtgctaatg
    10381 ccgtctgaaa taaaggcgcg tttgcggtat ttcaatcttt tggggtgtat gttttatgaa
    10441 tttggaacag ttgggcaggc gggacgcgct gctttccgga cttttgaaac aggcgggaca
    10501 gtggcggcgg ttggatgctg ccgtgaaaaa actgctgcct gccaacctgc acccccactt
    10561 tcagacggca tgtatcgaag acggcaggct tgtccttttg gcggcaaaca atatggcggc
    10621 atcgcgtttg aagatgattg ccccgtcggt attgccgcag ttggcagggc ttgatgcttc
    10681 gatacggtct gtttcggtca ggctggttcc aaaaccggaa aaaccgccga aaaccaatac
    10741 cctgcatttg agtaaggctg cgttggagag tttcgattcg gcggcggcaa agttggaaga
    10801 acggcatccc gaattggcgg aggcactgga ggagctggtc aggaagtacg gggcataagg
    10861 cggccggcag ggatgttgta aatatccgtt aatatttgcg cggcggggga cggctgccct
    10921 aatattaaag aaataggttc ggggtaagat tgcccttttt ttgggtaaac gattgtaaac
    10981 ttgcaaacag gctttgattt caaatgaagt ttgtagcaaa atgccgcccc gaaacatctg
    11041 tttgtgcaac acggcggaat ctttttcaag gttttgttaa tggcggttgc actttgattt
    11101 ccgtaaaacc gaatattatt ttatcgattg gagatttacc atgaaagctt atctggctct
    11161 gatttctgcc gccgttatcg gtttggctgc ctgctctcaa gaacctgccg cgcctgctgc
    11221 cgaggcaact cctgccggtg aagcacccgc ttccgaagcg cctgccgccg aagctgctcc
    11281 tgcagatgct gccgaagccc ctgctgccgg caactgtgcg gcaactgtcg aatccaacga
    11341 caatatgcag ttcaacacca aagacatcca agtcagcaaa gcatgtaaag agttcaccat
    11401 cactctgaaa cataccggta cgcaacccaa agccagcatg ggtcacaacc ttgtgattgc
    11461 caaagctgaa gacatggacg gcgtatttaa agacggcgta ggtgctgccg ataccgacta
    11521 tgtcaaacct gacgatgcgc gcgttgttgc ccacaccaaa ctgatcggcg gcggcgaaga
    11581 gtcttccctg actttggatc ctgccaaatt ggctgacggc gactacaaat ttgcctgcac
    11641 cttcccgggt cacggtgctt tgatgaacgg caaagtgact ttggtcgatt aatccgctta
    11701 aagtttcaaa agacagacag cctgctttgt gcaggctgtt ttattataaa atgactgctt
    11761 gaaaagtccc cgttgagaac gaaaacatga atccgtttga aacccaaagc gttacctttg
    11821 ccgaaccgat tgaaatgctg tatgcctgcc acggcaaagt gcgccgtttt tgcggccaga
    11881 ttgctatgtt gtcgggctat atcgccgaaa acggctgcaa tcagcttgtt ttgcaaacca
    11941 tccgccaaat ctcccggtat ttcaacgttg ccgcgccgct gcaccatgag gacgaagaag
    12001 aaaacttttt cccgctgctg ctgcaatacg cgccgcaagc ccgtgaaggc gtggacgagc
    12061 tgttgcgcca acatatcggg ctgtacgaca actgggcggc tgtttccgcc gaatttgcca
    12121 aactcgaagc agacaacgct tatatccccg atgcggaagc gttcaaacgc tttgtggagg
    12181 gatatgatgt gcatctggcg attgaagagc ctttgttcga catgggcaaa acgtttatcc
    12241 ccgaagagaa gctgaccgaa atcggcgaaa tcatggcggc gcgccggtgc aaataatgcc
    12301 gtctgaaaca atcgtctcaa ggatatatta tgctgacgcc caaaagctgc gatttgttca
    12361 atatcccgtt tttccagttt gcccagctca aaaaatacca gcccgagagc attccgcaaa
    12421 tcaaagccga ctacaaggaa aactggcaga tatggcagca gttgatacag caggtcgccg
    12481 cagatttgag cgaacctttc gccccgccgc atatcgaacg ctggtgtaac ggctggcagg
    12541 ttcgcgccca tttcttcgcc tacttcaaat acgcccaata caaaaattcc gccgccatcc
    12601 tgtcgatact gctcaaccgc cgccgcctga gcgtcagctt ggactggcat tgctacaaag
    12661 ccgacgtgtc gccgattgcg ctgcccgagt acaaccgctg gctggatgat ttcgataccg
    12721 aaaaatacgc cgcattcgat atgtggcacg gcgcggaaag cgaatatgac gactaccgca
    12781 ccgtcgcaca acaaagcgaa tccgaccgaa ggttgcaaaa cgacgaagac tttttctgca
    12841 tcggcaaaca cattgagcgc gacgatttgg gcaagcagga tgtagcaaaa tggatagccg
    12901 aaacggtgga agatttactg ccgctttacg aagcctgtca cggcaaatga ttcagaaaac
    12961 tgacgggtac gaatgcccgt cttatacacc aatccaaccc ggaggtcgtc tgaaaatcag
    13021 cttcaggaca tttcagacga cctgatttcc aaagtgaaca aaatgaccga aacccaatcc
    13081 ctagaactcg ccaaagcgtt gatttcccgc ccgtccgtta cccccgacga ccgagattgc
    13141 caaaaactgc ttgccgaacg cctgcacaaa atcggttttg cggctgaaga actccatttc
    13201 ggcgacacca aaaacatctg gttgcgacgc ggcacgaaag ctcctgtcgt ctgttttgca
    13261 gggcataccg acgttgtgcc gacaggtcct gttgaaaaat gggattcgcc cccgttcgaa
    13321 ccgaccgagc gcgacggaag attatacggg cgcggcgcgg cggacatgaa aaccagcatc
    13381 gcctgtttcg ttaccgcctg cgaacgcttt gttgccgaac atcccgacca tcaaggcagc
    13441 attgcactct tgattacttc cgacgaagag ggcgatgcac tggacggcac gaccaaagtc
    13501 gtcgatgtgt tgaaagcacg cggagagttg attgactact gcatcgtcgg cgaaccgacc
    13561 gccgtggaca aattgggcga tatgattaaa aacggccggc gcggttcgct gtcgggcaat
    13621 ctgaccgtca aaggcaagca agggcatatc gcctatccgc atttggcggt gaatcccgtg
    13681 catacttttg ccccggcctt gttagagttg acgcaggaag tttgggacga aggcaacgaa
    13741 tacttcccgc cgaccagctt tcaaatttcc aatatcaacg gcggcacagg cgcgaccaac
    13801 gtcattccgg gcgagctgaa cgtcaaattc aatttccgct tctccacaga gtccaccgaa
    13861 acagggctga aacaacgcgt ccacgccatt ttggacaaac acggcgtgca atacgatttg
    13921 cagtggtcgt gttcgggaca gcccttcctg acccacgcgg gcaaactgac cgacgtggca
    13981 cgcaccgcca ttgccgaaac ctgcggcgtt gaggccgaat tgtccaccac cggcggcact
    14041 tcggacggac gcttcattaa agccattgcg aaagaactca tcgaattagg cccgtccaat
    14101 gccaccatcc accaaatcaa cgaaaatgtg cggctggacg atattccgaa gctgtcggcg
    14161 gtgtatgaga ggatattggc gcggttgttg gctgaaaagg ccgtctgaaa agaagatggg
    14221 ctggttgtcg gatatggatg tccgacaatc tgattagacc caacatgagt ttgagataac
    14281 gcattgattt ttatgtagag cgatttattt gaacggtaat ccattccctc tcccgtggga
    14341 gagggttagg gagagggcat tgggacggca acggaaatat tgtctgtatc tgccacgttt
    14401 taccctccct ccgaccctcc ccacagggga gggagctaag ttgcaggcac accgacggaa
    14461 tatggttgaa accctgccct tcagggcagt agaattttgg tttatttttt gggaagtaac
    14521 cccttccaaa tcagggtaaa cagggctgcg ccttatgtgc ggccctgtgt gttgaaacat
    14581 tgaggaatat aaccgtgccg tcaaattact ttcatacgcg tgaacgattg ggtgctttag
    14641 gcggcatcgt gcaacatcat accgccttgt accgcagcgt ggcggtatat gaaccgaccg
    14701 tggttatcgt gcggcgcgga tgcaagaagc tgcggtgggc ggggcgcgaa ttgaggattg
    14761 cggcgggtga ggctgtcggc ttggcaggcg ggcagacgct tgacgtcatc aatattcccg
    14821 attcagacgg cctctatcag gcgcaatgga ttgctttcga acaggaaacc gtagaaaggt
    14881 tcgccgcaca atacgggacg gcgcaggcag tgtgcgatgc ggtgaagctg ccgcaccccg
    14941 ggcgtatggg cgcggcgttt gattatgcgg ctgcggtact ggcggatgaa gaagtgccgc
    15001 acaatgcggc ggaggccgcg ctttgcggcg tgttggcgtg gttgcagcat gacggcatcg
    15061 gttttgccgt gtacggaggc gtcaacctga tgcggcaaat ccgcaaactg attactgccg
    15121 atatggctgc ggactggtcg tcggcaatgc tggcgcagcg gcttaattgc agcgaggccg
    15181 cgttgcggcg gcggttggcg cggcaggaca cgaatttccg tacgctgctg acggatgtac
    15241 gcatgatgcg cgcgctgacg ctgttgcagg ttacgcaatg gtcggtagcg cagattgccg
    15301 gcgcggttgg ctatgactgc ccgtcccgtt tcagcgcgcg cttcaaagag ctgttcggtt
    15361 gcgtaccgtc ggtggtccgc tcggaagcgg agccggcggc atatcggcgc acggggcagg
    15421 tcagcgtcgg tgttcgaccg tgaatttcgc ttcgcccaag ctgtttgcct tagtggagaa
    15481 accgaccagc acgggtgtgg attcggcggt gatgttcggc agcttggcga ctttcagcgc
    15541 ggtaagcgtg aattcgtagc ggtgtttcct gccttcggga ggacacgcgc cgccgtagcc
    15601 cggcgcgccg aagtccgtgc gggtttgcag cgcgcctttg ggcggcctgc ctcattgtgc
    15661 ggtaatgcct gcgggcaggc ggcggacatc ggcgggaatg tcggcgacca cccagtgcat
    15721 ccggtccaga ccgatcggcg cgtctttatc gtaaacggtc aggacgaaac tttttgtccc
    15781 tgcgggcggg ttttaccacg acagcgcggg cgaagcattg ccgcccgaac agccgaagcc
    15841 gtaaggcgcg ctcaaaagct ggttttgcgt gaagccgccg tcttcggacg ggttgtcgaa
    15901 ttgcagcgtg aatgcgcccc cggcaaaggc agcggttgaa aaagccagca gtacggcgga
    15961 gaggatgcgg gtttgcatag atgattcctt tgcttgatga ataagtggat tcattatagg
    16021 ctgtcaacgg cggcaggaat gtgccgaaac ggttggatga tgtgccggaa tgcgcaaatg
    16081 caaaaatcgt ggtttcagac gaccctttga aggccgtctg aaatttttca tagggcgacg
    16141 ctttatgtgt cgtcctgtgt gttgaaacat tcctttccct gatgctttgg aaatattgta
    16201 tatttaggca ttgccgcaac cagaccctca aggtttccgc cgcagcctgt tcctgtaccg
    16261 gcgtgcggga atggcggaaa tcggggattt cgccgtcaaa tttatgatag ggatacggag
    16321 caaacgaatg attaccctgc cttccttaaa caatctgccg tcaaaatggg atgaaatccg
    16381 ccattgcctc gaaacccggg tttttgagtg cggcgttatg ccgtatccca atctgacgga
    16441 atgcgaagcc gggcagttcg agcgggattt ttgggacaat atcggctgcg cgcctgaaga
    16501 atatgtccgt atccgccgcg ccatccgtct gttggaatta cgttatcccg acagcttgaa
    16561 cgagctggtg tgtgccgcca ttgccacgcc tttgggcgag atgttggcgg tgttcggcgt
    16621 taagggattg tgcctgctgg agtttgtcgg acagaagtat ttggaacagg aaattgccgc
    16681 cgtccaaaaa gccttgcgcg gacggtttgt gtttcgggaa gacgggcgga tgcaattttt
    16741 gcgtcaggaa ttggacttat acttcaaggg tcatctgaaa acctttgcca cgcctttgga
    16801 gcagattggc accgagtttc aaaagcaggc atgggacgcg ctcttggcga ttccttacgg
    16861 cgaaacgcgc agctacaaag agcaggcgca gcgtttgggc aaccccaaag ccgtccgcgc
    16921 cgttgccgcc gccaacgggc agaacaaggt gtctgtcatg attccctgcc accgcgtcat
    16981 cggcagcgac ggcaaactga ccggctacgc gggcgggttg aaccgcaaac agtttttact
    17041 ggctttggaa cgcggcgaag ttcagacggc attgttttaa gcggggaaat gcagaacggc
    17101 tcatcgggct taaatatggt gtgatgacgg ctgtgaaaat tcatacaacg gcaatccttt
    17161 taaggcatcg gtcttaatgt ctttctgatt catatgcttg tcgttttgtc gcccattttt
    17221 ccaaaattaa ggattgacac attttttccg gacgctataa tacgccccat tccccgatag
    17281 ctcagtcggt agagcgacgg actgttaatc cgcaggtccc tggttcgagc ccaggtcggg
    17341 gagccagaat tttgaagccc gaacttgaat gttcgggctt tttcacattt gcacgtccgg
    17401 cgctcggaat gcggcgagat aggttttcca acggtttcgc ggtaaaattc tgcctgtctg
    17461 atttcggttt cgttttatgt ctatcaaaat cctgattatt tctcccagtt ggataggcga
    17521 ctgcgtgatg acccagccct tgttccgccg tttgaagaaa cttcatcccg gttgcacgat
    17581 tgatgtgttc gcaccgaagt ggtcgatggc ggtgttcgag cgtatgccgg aagtgaatga
    17641 gattcttgaa aatccgttcg gacacggtgc gttggagctg aaacgccgtt ggcgggtcgg
    17701 cagggagttg ggacggcgcg gatacgacag ggttatcgtg ttgccgggtt ctttgaagtc
    17761 ggcagtcatc gcgctggcaa cgggcatcgg aaaaagaacg ggttatgtcg gcgaaagccg
    17821 ttattttctg ttgaacgata tacgcaggct ggataaggaa cgtctgcctt tgatggtgga
    17881 ccgatatacg gctctcgcgc atcagagtca ggaagatttt gacgggcatt cgagattccc
    17941 cgagttttcc attgatgaac ggcggcggga aatttctgtc gaaactttcg gtttgaatct
    18001 tggaaagcct gttctggctt tttgcccggg tgcggaattc gggccggcaa agcgttggcc
    18061 ggcaaggcat tttgccgagt tgggcaaaca ctattcggag gcgggttggc aggtttggtt
    18121 gttcggttcg caaaaagatg atgaaatcgc cgaagaaatc aactgccttt cagacggcat
    18181 gtgtgtcaat ttgtgcggca aaaccgattt gtcgcaggca atggatttgc tgtcgttggc
    18241 ggacacggtc gtgtgcaacg acagcgggct gatgcatttg gcggctgctt tgggcaggaa
    18301 ggtagtggcg gtttacggtt cttccagccc gacgcatacg ccgcctttga gcgacagggc
    18361 gaaaatcgtc agcctgcact tggaatgttc gccctgtttc aaacgtgaat gcccgttggg
    18421 gcataccgac tgcctcaaca ggctgtatcc cgagaagatt gtgcaggcgg ttgaagaggc
    18481 ggtatgatgg cttctgtttt cggatttata ccgtctgacg gcggatattg ataggatgtt
    18541 tatggctatt gcgaacaata aaaaagcatt tcacgatttt ttcattgaag accggattga
    18601 agccggtttg gtcttggaag gctgggaagt caaagcaatc cgcgccgcgc gcgtacagct
    18661 taaagagagt tatatctatt ggaaaaaaga tgcgttttat ctggtcggct gccatattac
    18721 ggctttgcct acggcttcga cacacatcaa accggatgcc gtacgtccgc gcaaactgtt
    18781 attaaaacag tcggaaatca ataagttaat cggaaaaacc gaacgtgcag gttacaccat
    18841 cgtgccgctg gatttgcatt tttcacgcgg aaaaatcaag atggaaatcg gtttggcaaa
    18901 aggtaagaaa cagcacgata aacgacaaag tatgaaggaa gccgactgga aacgcgagaa
    18961 acagcggttg attaagcata cgcgctgatt gtggaaacaa tgcttcagac ggtacgggca
    19021 aggctgccgc ctgatgcgta aacgttctac acctaaacca gcatccgctt taatcagcac
    19081 ggcatgatgc cccaaacctt cgtcaaaggt ttgagcaatt tgatgataaa ttggcattag
    19141 ccaagggtaa agtgcggtca tttttcctga aatttttgta aataacgagg gcaattatac
    19201 acattttcaa accggcttga caaaatagca aaaagcaaac gtttactttt aataaataag
    19261 aaataaatag agaaaatttg aactaaatga cattttttgt tcagttttat gctaaaatcc
    19321 acgccctatt tatttttact tgacaaagga aaatttatta tgaaactcga agcaagcaag
    19381 cagaagttta aaaaatcatt tattataagt ctattttttt ctattcttta tacctctccg
    19441 cttttggctg ttgattacgt ttacgacaat tctaaattaa caaatgagca aattgatcgc
    19501 ttaaaaaaaa cttagagata aaagcagcga atactggaaa caagaaactt atcttttaaa
    19561 accgacgaat ggtagctctc agaactctct caaagttccc gcactatcct ttaaaacaca
    19621 ccaattcgaa aacatcaaca actcaaaaaa gatttctttt tatgacaagg aatacactga
    19681 agattacctt gttggcttcg cccgaggttt cggggttgaa aaaagaaatg gggaagaaga
    19741 aaaaccactc cggcaatatt ttaaggactg tgtaaatact gagaattcca ataatgataa
    19801 ttgtaaaatt tcatccttcg gaaactatgg cccaatatta ataaagagcg atattttcgc
    19861 cttagcatca caaataaaaa atagccatat caacagcgaa attttatctg taggtaatta
    19921 tatcgaatgg cttcgcccaa cacttaataa gcttactggt tggcaagagc atctatatgc
    19981 tggtttagat ccattccact atattgaggt tacagacaac tcgcacgtta tcggacaaac
    20041 gattgatttg ggagcattag aattaacaaa ttctctatgg gaaccccgtt ggaactctaa
    20101 tattgattat ttaataacaa aaaatgctga aattcgtttc aacactaaaa atgaaagttt
    20161 actcgtaaaa gaagattatg ctggcggagc tcgttttcgt tttgcttacg atctaaaaga
    20221 taaagtccct gaaataccag ttttaacttt tgaaaaaaat ataactggca catcagatat
    20281 tatttttgag ggaaaagcgt tggataattt gaaacaccta gacgggcatc aaattgtcaa
    20341 agtaaatgac accgcagata aagatgcctt tagactttcc agtaaatacc gaaagggaat
    20401 ttatacgctc tctttacaac aacgcccaga gggctttttt accaaagtgc aagaacgcga
    20461 tgatattgcg atttatgcac aacaggctca agccgccaat accttattcg ccttgcgttt
    20521 gaacgacaaa aacagtgata ttttcgaccg cactttacca cgcaaaggct tgtggttgcg
    20581 tgtgattgac ggacattcca accaatgggt gcaaggtaaa cagcacctgt agaaggctat
    20641 cgaaaaggtg tgcaactcgg cggcgaggtg ttcacatggc aaaacgaaag taatcaactt
    20701 tctattggct taatgggcgg acaagcagaa caacgcagca ctttccgcaa tccggataca
    20761 gacaatctta caacgggaaa tgtgaaaggc tttggtgcgg gcgtttacgc cacttggcat
    20821 cagcttcagg acaaacagac aggtgcgtat gttgatagct ggatgcaata tcaacgtttc
    20881 cgccaccgca ttaacactga atatgctaca gaacgtttta cttcaaaagg tattactgcc
    20941 tcaattgaag cgggttacaa tgcgttattg gcggaacact tcactaaaaa gggcaacagc
    21001 cttcgtgttt acctacaacc acaggcgcaa ttgacttatt tgggggtaaa cggaaaattc
    21061 agcgatagcg aaaatgccca agtgaatttg cttggctccc gccaattaca aagccgagtg
    21121 ggcgttcaag ctaaagcgca atttgctttc actaatggcg tcactttcca accatttgtt
    21181 gctgtcaatt caatctacca acaaaaacct ttcggtgtgg aaatagacgg ggatcgtcga
    21241 gtgataaaca ataagacggt aattgaaact caattaggtg tagcagcgaa aatcaaatct
    21301 cacttaacct tgcaagcctc attcaaccgc caaacaagca aacatcatca cgctaaacaa
    21361 ggtgcgttga atttacagtg gacgttttaa gttaaattgt aacaaaataa aatcggcagg
    21421 gaaattcact gccgatttta ttattttaaa agtgcggtca tttttcgtgt aatttccacc
    21481 aatttttacc gcactttcaa tttcagcttg aacaagttca atagtctgct ctgcattaat
    21541 tacgactgct tttggattat cttttactaa ttctaaatag cgtgctcgag tgcgatggaa
    21601 aaaatctaaa tccatttgct caatacggtc taactcgcca cgtccacgag ctcgcgctaa
    21661 accgacaatc ggatctattg tccttttctt tgttgtgtat gcaaatcaga aatgccggtt
    21721 acggaagcag tttgccagga ttcataatgt tatatggatc aaggtgttgt ttgatgcttt
    21781 tcatcagggc aatttcagag ggcgtgcgga cagcgggcag ccattgtttt ttgatgatac
    21841 ctatgccgtg ttcggcggca atcgtgccgt tgcgggcaag gacgttgcga tagactgtgc
    21901 tgttgatgtc gttttcgtaa cggtaaactt cattgctgag gatttcgggc ggaaaagtat
    21961 tgtagtgcag gctgccgtcg cccagatgtc cgaagcagac gatttgtatg cctttgaaat
    22021 tctgttccaa atctttggcg caccggcgga caaagtcggc aacgcgcccg ataggaacgg
    22081 caatatcgtg tttgatgctg gtgcccagtt tgcgttggga ggcggagatg ttttcgcgca
    22141 acgcccacat atggatacgt tcttgttcgc tttgcgccaa tacgctgtcg gtaaatcctt
    22201 ttttataaag aaattcgaca agccgatcat caagattgct gtcgggtaat gagtcggtca
    22261 actcaagtaa aatatgccat tctgaatgtg tcggcagggg gagtttgctg aattcggaag
    22321 acaactcggc ggcaaaacgg ccgatcagtt caaaactgca taagcgttcg gcaaagtgcg
    22381 cttgggtttc ggtcagcagg cggacggcgg attcgatgtc gggaatgccg acccatgccg
    22441 ttgccttgtc cgagggacgg gcaaaaagct tgagcgtggc ggcagtgatg atgcccagtg
    22501 tgccttcgct gccgataaac agatggcgca ggtcgtagcc ggtggtgttt ttatgcaggg
    22561 gatggagatg ggaaaccagt tcgccgttgg ggaggacgac ttccaaaccg ataaccaggt
    22621 cgcgcatcgt gccgtaacgc aatacgttca aacctccggc attgcaggcg atgttgccgc
    22681 cgatttggca cgagccttcg ctggcgagac tgagtgggaa cagcctgttt gaggcttcgg
    22741 ctgcctgttg gacggtttgg agtacggaac ctgcttcgac ggttatgcag ttgtctgaca
    22801 aattgatgct gcggatgcgg ttgagtttgg aaaggttcag caatacgccg ttttccgata
    22861 ctgccgcgcc gcacaaaccg gtattgccgc cttgcggcgt aaccggaatg cggtgttcgt
    22921 aacaaaaacg cataatggtt tgcacgcttt caacgctgcg cggctgcaaa atgatgtctg
    22981 gtgcagacgt aaagcggttg cgctgatctt ttaaaagcgt cggagaaggt tcggcaattt
    23041 catctgccgg caggagtcgg gaaaattcgg tatgaaggtc aggcatgttt gtcggaaggg
    23101 tagggagaag attgaggaag ggaatctgat gccgtctgaa aaatcaaaaa agcaggatac
    23161 tgaacgtatc ctgctttttt gcaagcggaa aatgcttatt ttgcagcttc ggcagcagga
    23221 gcttcagcgg caggtgcttc ggtagcagca gcttcggcgg caggagcttc agcagcggga
    23281 gcttcagcag cgggagcttc agcagcggga gcttcagcag cgggagcttc agcagcggga
    23341 gcttcagcag cgggagcttc ggcggcaggt gcttcggcag caggtgcctc ggtagaggaa
    23401 gcctcagcag caggagcttc agcggctttt tctccgccgc aggcagccaa agccaaagac
    23461 aacaaagcag cggcaaacag ggattttttc attttagtaa cctttaaaat caatttaaat
    23521 tattgaaaca aataggtctt tttcgaccat gctacaaacc gggttggaaa ttcatcgctg
    23581 aatttgcgta attatgcacc atggaataag ggattgcaat gtcgtacaga tgggaaaaag
    23641 gtttgaatga aaagttttct ggcgggcgga ttgataaatc ctgtaaaacg gttttgctgt
    23701 taaaaatccg atagggataa atattataga gattaatata attcagtatc ttgtatgatt
    23761 tattaaggca gattggggaa gtcggcagat gggtcggaat ggtttttgag agtgttgtaa
    23821 aaaatttaca ctgcaataaa ataacaaaaa tttacattga aatatgtgcc gtacagttta
    23881 tgaacaggtt aacggtaatc ggccggcgcg gcggttgtgt tagaatgcgc ctgtaatcaa
    23941 tctgctgttc ctgaaaggac aaatatggct attgcaaaaa attccgtggt ttcgctgcat
    24001 tatgagatgt atgatgccaa caatcagctt ttggataaaa ccgaagaacc gattgcgtat
    24061 ctgcacggcg gttacgacgg cattttccct ttggtggaag aggcgttgca cggtaaggat
    24121 gccggcgata cggtcgatgt ggcgctttcg tccgacgatg cgttcggcga gcaggattcg
    24181 gagttggtcc gtattgaaga tgcgggcgcg ttccctgttg aagtcgaagt cggcatgatg
    24241 tttgaagccg acgatcctga aaccggcgat gttgtcgtct atcgtgtaac cgatgttgcc
    24301 gacggcaagg cggtggtgga cggcaaccat cctttggcag gcatgaaaat ccgctttaaa
    24361 gctacggttg aaagcgtgcg cgatgcatcc gatgaggaaa tcgcacacgg tcatgtccac
    24421 ggttcgcacg gtcattacca ccactgattg ccggcagctt ggaggtatct gatgccgtct
    24481 gaaaagggtt tgttccgttt cagacggcat tttgtttgat ggatggagaa ggtatgtcgg
    24541 ttgggcatta tgagaatttt cccgtcggtt cgctggtttt gccgcgcagg ttgaggaagc
    24601 cggttcatgc ggtgtatgcg tttgcacgga cggcggacga tatggcggac gagggcagta
    24661 tgccgtctga agccaggttg tcggggttgg agggtttgcg gcgcgagttg gacgtgttgg
    24721 catcgggcgg ccggtcggcg catcctttga ttgcacgatt ggatgccgag gcggttgtgc
    24781 cgttcggttt ggatttgcag ccgttttatg atctgctctc ggcgttttcg caggatgtgg
    24841 ttaaaacgcg gtacgcgcat ttcggcgatc tgaccgatta ttgccggcgt tccgccaacc
    24901 ctgtcggacg cattatgctg gctttatacg ggaaaacgga tgcggtgtgc gtagcgcaaa
    24961 gcgacggtat ttgtacggct ttgcaactgg tgaatttttg gcaggatgta gctgtggatt
    25021 ggcaaaaggg cagggtttat atcccgcagg acgatttgtt gaaattcggt gtttctgagg
    25081 aacagatcgc agcaggacgg gcggatgcgg cgtttcagcg gctgatggcg tatgagtgcc
    25141 gacgtgcatt ccgtatgctg aaggcgggtt cgcctttggc gcgcgaattg aaagggcgta
    25201 tcggtttgga actccgtatg attgtgttgg gggcgcagtt gattttgcag aaactggacg
    25261 cgtgccgata cgatgtgttt gcacaacgcc ccgttttgga taagaaggat tggttgatta
    25321 tgttgaaacg cgcgttgtgg aaatgattgg gggaaaatga atgccgtctg aaaggggttg
    25381 ttccgcttca gacggcattt tgcaggcggt cggtatggtc aggcgatttc atgcgggttt
    25441 aacctggctt gatcctctgc acccaagccg atgagtttgc gcagtcgggt catgtagaaa
    25501 ttgacatcca aaccggtgcc gtagcgttgc gccgtccaca gtgtttccgc cagtgcctcc
    25561 atcatttcgt gttcggcttc cggccagccg cgtttggcgc acagggtgtc gtggatggcg
    25621 cgtatgccgt gcggctggtc tatgcccgcc tgttcttgga cggacaaatg cagcgacata
    25681 tgcaggaagg ggttgctttc gccgttttcg ggcagccagt cggtgtccag atggtcttcg
    25741 atgcgttcga gataacggtg gtattcggga tgggcttcga caatgcggag ggctttctgt
    25801 tccaacgcgc ccaattgcag cggattgagc cgctgctgcc acacgcgggc gaaaaagcgg
    25861 cggacatcgt gggtattgac atcgtacatg gcgggtccga taagatgttt atggaaatag
    25921 gttcggataa tggcgggttt tacataaatt aaatcctacc gtcaacctta ccatttataa
    25981 tgcggacaaa ttatttgtct gaaacggatt cgattatgaa aaaactgatt tgcctgttcg
    26041 ccgtattttt gatgttgtgc ggacgggctt tcgcgctgga tgcgaacgat ctgctgccgc
    26101 cggaaaaggc attcgcgccg gagcttactg ttgccgacga cggcgtgaac gtccgtttca
    26161 ggattgccga cggatactat atgtatcagg cgaaaatcgt cggcaagacc gatccggcgg
    26221 atttgttggg acagccttct ttcagtaagg gcgaagagaa ggaagacgag tttttcggca
    26281 ggcagacggt ttaccatcac gaggcgcagg ttgcctttcc ttatgcaaag gctgtcggcg
    26341 aaccgtataa attggttttg acctatcagg gctgtgccga agccggcgtg tgctatccgc
    26401 ccgtggatac cgagtttgat atttccggca acggcactta ccatccgcaa accgacgaac
    26461 cggcatccgc caaagaccgt tttttgcagc cttcctctca aaacggcagc ggggcgttgc
    26521 cgcccccgaa gggggacgag ggcggcgacg gccgtttcaa gctgtcttgg gatacgctca
    26581 acgccaatct tttggcgttt tttctcgccg gtttgggcct gagttttacc gcttgtatgt
    26641 atcccctgct gccgattgtg tccagtatcg tggtcggtga caaaaaggcg ggcaaggcgc
    26701 gggcgtttgt gctgtccgtc gtttatgttc agggtttggc tctgacttat acgctggtcg
    26761 gcattgttgc cggactgacg ggcgcgctgc tgaccgtatg gttgcagcag gcttgggtcg
    26821 tgttggcggc atcggctttg atggtcgtct tggcgctgtc tatgttcggg ctgttcaaca
    26881 tccagcttcc caacgccgta cagtcatatt tccaaaacca gagcagcagg ctctccggcg
    26941 gtaaaatcgt ttccgtcttt attatgggta ttctgtccgc actgattgtc ggaccgtgcg
    27001 tcgccccgcc gctggcgttt gccttgggct atatcggtca gacgggcgat gcggttttgg
    27061 gcggtttggc actttacact ttggcgttgg gcaccggcgt gccgctgatt gccatcggca
    27121 cgttcggagg acatatcctg cctaaggcgg gcgattggat gaatgccgtc aaatacgctt
    27181 tcggctttat cctgcttgcc gtcgccgttt acctcgccac gccgcatttg ccctattatc
    27241 tcgtcgtcgc gctgtacacg ctgctgatgc tggttcctgc ctttatgctg ctggtcaacg
    27301 gacgcaggca gaaacgccgt ccgaaagctg tggcattcgc attgggcagc atattgctga
    27361 taggcggcgc gtggttcggc tggcagggcg caaacggcaa aacgaccgcg ctgcaccatt
    27421 tcttgaccct caatccgccg gccgaagcag gcaaattttc ggaacacggc aaaatgtttg
    27481 ccgatactgc cgcgctgaag gcagcgatgg atacggcgtt gaaagaacat cccgacaaac
    27541 ctgtcgtttt ggatttttat gccgactggt gcatttcctg caaagaaatg gcggtttaca
    27601 cgctcaatca gccggaagtg catcaggcag tcgatatgga acgctttttc cagatcgacg
    27661 taaccgccaa cacgcccgaa catcaggcgt tgttgaaaga atacggtctg ttcgggccgc
    27721 cgggcgtgtt tgtcgtccgc gccgacggca gccgcagcga gccgctgctg ggttttgtca
    27781 aagcagacaa gtttatcgag tggtatgaac aaaaccgctg attccgatgt ttcaaatgcc
    27841 gtctgaagtt ctcagacggc atttttgtca gacaggaaaa cggcaatatc cgctatacac
    27901 acggaagcag tccaaagatg tgaaaatcgt gtgtataagt cggtttgcgt attgaaatta
    27961 aaagataaaa atacgatgat taaaaaatag gcataagaaa agataaacac agccggtgtt
    28021 tgccggtgtg tgtatctttt gtggaaaaac atcttaatgc catgtatttt atggaaaata
    28081 ataacatgat taaaaaatag tcaatcatta aggttcaggt aggcggctgc aaccgtcaat
    28141 gtctgaaaag aggtagtgct gtttaagcat ggaaagcc
//
LOCUS       DAXFHF010000023        27968 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXFHF010000023 DAXFHF010000000
VERSION     DAXFHF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27968
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.023"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(194..2236)
                     /gene="recG"
                     /locus_tag="ACFIRM_000922"
     CDS             complement(194..2236)
                     /gene="recG"
                     /locus_tag="ACFIRM_000922"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC3028577.1"
                     /translation="MMSPETQKQLKITDASAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYAGHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMTPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2322..2765)
                     /gene="secB"
                     /locus_tag="ACFIRM_000923"
     CDS             complement(2322..2765)
                     /gene="secB"
                     /locus_tag="ACFIRM_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC3028578.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2786..3043)
                     /gene="grxC"
                     /locus_tag="ACFIRM_000924"
     CDS             complement(2786..3043)
                     /gene="grxC"
                     /locus_tag="ACFIRM_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC3028579.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3262..4755
                     /gene="rng"
                     /locus_tag="ACFIRM_000925"
     CDS             3262..4755
                     /gene="rng"
                     /locus_tag="ACFIRM_000925"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC3028580.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4790..6041)
                     /locus_tag="ACFIRM_000926"
                     /pseudo
     CDS             complement(4790..6041)
                     /locus_tag="ACFIRM_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6123..6752)
                     /locus_tag="ACFIRM_000927"
     CDS             complement(6123..6752)
                     /locus_tag="ACFIRM_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFC3028581.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAELKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLRKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(7019..8332)
                     /locus_tag="ACFIRM_000928"
     CDS             complement(7019..8332)
                     /locus_tag="ACFIRM_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC3028582.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8710..9282)
                     /locus_tag="ACFIRM_000929"
     CDS             complement(8710..9282)
                     /locus_tag="ACFIRM_000929"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC3028583.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9441..10850
                     /gene="dacB"
                     /locus_tag="ACFIRM_000930"
     CDS             9441..10850
                     /gene="dacB"
                     /locus_tag="ACFIRM_000930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC3028584.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10915..12084)
                     /gene="metK"
                     /locus_tag="ACFIRM_000931"
     CDS             complement(10915..12084)
                     /gene="metK"
                     /locus_tag="ACFIRM_000931"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC3028585.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSEPGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12267..12377)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12430..13323
                     /locus_tag="ACFIRM_000932"
     CDS             12430..13323
                     /locus_tag="ACFIRM_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3028586.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13368..14432)
                     /gene="tsaD"
                     /locus_tag="ACFIRM_000933"
     CDS             complement(13368..14432)
                     /gene="tsaD"
                     /locus_tag="ACFIRM_000933"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC3028587.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14548..15735)
                     /gene="ccsB"
                     /locus_tag="ACFIRM_000934"
     CDS             complement(14548..15735)
                     /gene="ccsB"
                     /locus_tag="ACFIRM_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC3028588.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15728..17680)
                     /locus_tag="ACFIRM_000935"
     CDS             complement(15728..17680)
                     /locus_tag="ACFIRM_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC3028589.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVAGATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17958..18581)
                     /locus_tag="ACFIRM_000936"
     CDS             complement(17958..18581)
                     /locus_tag="ACFIRM_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3028590.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18788..19417
                     /gene="yihA"
                     /locus_tag="ACFIRM_000937"
     CDS             18788..19417
                     /gene="yihA"
                     /locus_tag="ACFIRM_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC3028591.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19537..21933)
                     /locus_tag="ACFIRM_000938"
     CDS             complement(19537..21933)
                     /locus_tag="ACFIRM_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC3028592.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVSAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            22085..23200
                     /gene="pilM"
                     /locus_tag="ACFIRM_000939"
     CDS             22085..23200
                     /gene="pilM"
                     /locus_tag="ACFIRM_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC3028593.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23203..23802
                     /locus_tag="ACFIRM_000940"
     CDS             23203..23802
                     /locus_tag="ACFIRM_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC3028594.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSKIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23803..24450
                     /locus_tag="ACFIRM_000941"
     CDS             23803..24450
                     /locus_tag="ACFIRM_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC3028595.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24468..25013
                     /locus_tag="ACFIRM_000942"
     CDS             24468..25013
                     /locus_tag="ACFIRM_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC3028596.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            25032..27203
                     /gene="pilQ"
                     /locus_tag="ACFIRM_000943"
     CDS             25032..27203
                     /gene="pilQ"
                     /locus_tag="ACFIRM_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC3028597.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPFTESVVSVSAPFSP
                     AKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTL
                     KNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKSAAPGYFTF
                     QVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNG
                     KMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADL
                     GALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTR
                     SVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLKNETSAFGW
                     GVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESLSKTKTLAN
                     PRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNITPDGQIIM
                     TVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNGNTLTKVPL
                     LGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT         7089 a         8072 c         6725 g         6082 t
ORIGIN      
        1 aaaaaccggt acggcgttgc cccgccttag ctcaaagaga acgattctct aaggtgctga
       61 agcaccaagt gaatcggttc cgtactattt gtactgcctg cggcttcgtc gccttgtcct
      121 gatttttgtt aatccactat aaaaaatcag atttccgtta cacttttttc taatatttca
      181 gacggcattt tgctcacaca cccaaataac cttccctgcc ggaaagccac cttgccaaat
      241 gcgcttcgac gatttcaggg ttttgttcaa tcagcatcgg ggcggtttcg cgcgcttgtt
      301 ccaaaaggtg taagtcttct tcgagcttgg cgaagcgcag cataggcacg ccgctttggc
      361 gcgcgccgag aaattcgccg gggccgcgga tgttgaggtc ttggcgggcg atttcgaagc
      421 cgtcggtgtg ttcgtagatg actttcagcc gcgctttggc gagttcgccc aagggttcgg
      481 caaacaggag gacgcacacg ctttccgccg cgccgcgccc tacccgtccg cgtaattggt
      541 gaagctgcgc caagcccatg cgctcggcgt gttcgatcac catcagggcg gcgttgggca
      601 catctacgcc gacttcgatg acggtggtgg caaccaagac gttcagacgg cctgcggcaa
      661 attccgccat gacttcagcc ttttcggcgg ccttcatgcg cccgtgtacc agtccgatat
      721 tgggttcggg caatgccgtc tgaagccggg cgagggtttc ggcggcggtt tgcagttgca
      781 gggtttcgct ttcttcaatc aatgggcaga cccaatatgc ctgccgccct tttcggcaag
      841 tgccgaggac gaagccttcg acttcggcgc ggcggacgtt gttgacgagg cgcgttttaa
      901 tcggcgtgcg cccgggcggc aattcgtcga tgacggatac gtccaagtca gcgaagaagc
      961 tcatggcgag cgtgcgcggg atgggtgtgg cggacatcat cagctgatgg acttcgcgcc
     1021 ctttgttttt gagtgcgagg cgttgtgcaa cgccgaaacg gtgctgttcg tccacaatgc
     1081 tcaagcccaa attgtgaaac gccacgccgt ctgaaaacag ggcgtgcgtg ccgacggcga
     1141 ttttgacgct gccgtcggcg agtttggctt tggcttcgtc tttggctttt ttgcgcaaac
     1201 tgccaaaaag gcagacaact tcaatgccca aaggttccag ccattgtttg aacttgatga
     1261 agtgctgttc ggcaaggatt tcagtgggcg tcattacagc cacttgcgcg cctgattcga
     1321 tggcggtcag cgcggataag gcggcgacga tggttttgcc gctgccgaca tcgccttgca
     1381 gcaggcggtg catggggtgg gtttgcgcca tatcgcggca gatttcggaa acaacttttt
     1441 cttgtgcatc ggtcagggca aacggcaggg cttggcgcag ggcttgggtc aatgtgccgt
     1501 cgccgcgcaa tgccgccgcc gtgccgctga cgcgcttctg ccgcgccaaa cgcatggaca
     1561 actgttgcgc caaaagttcg tcgaatttga gccgctgcca cgccggcagc gtgccgtctg
     1621 aaagttgatg aatagtgaaa ctcggcggcg gcgaatgcaa aaggcgcagg ctttcggcaa
     1681 ggtgcggcag cttcagacgg cacagcaggg catcgggcag cgtgtcgtgc agcggcgtaa
     1741 cgtccaacgc cgtctgaatg atgcggcgca aagtgggctg gttcaaaccg tttacggtcg
     1801 ggtaaaccgg cgtgaggctt tccgccaaac cgccgccctc ggcatcgcgg attttgggat
     1861 gaatcatctc gtcgccgtaa aatccgtgtt tgatttcgcc cacggcgcgg atgcgtttgc
     1921 cgaccgccgt ctgcttctga tgtccggcgt aaaaatggat gaagcgcaga aaaaggacgc
     1981 tgccggaacc gtcggcaatt tggacaatca gctgcttgcg cggtttgaac gttacttcct
     2041 gatggataac ctcgccctcg acctgacacg gcacgccaat cggcgcgtcc ttaatcggca
     2101 taatgtgcgt ctcgtcctcg taacgcagcg gcaggtgcaa caccaaatcc cacgcggtat
     2161 ggaggttgag tttgtcgagc ttcttggcgg aagcatcggt gattttgagc tgtttctggg
     2221 tttcgggcga catcataggc agattccttt ggacgcgcct attttatccg aaaacaaaaa
     2281 tgccgtctga aacggattca gacggcatcg acaggcagga atcaagcccc ggcggcttcg
     2341 gcttcctgct gttgttggta aatcgcttca aaattaatcg gcgcaagcag gacgggcggg
     2401 aagccggcgc gcgttaccgt accggaaaca gcttcgcgcg cgtaagggaa gaggatgttc
     2461 ggacacgcca cgcccaacag cagctgcaca tcttcttcgg ggatgttttc cagacggaaa
     2521 ataccgcttt gggttacttc gttcaaaaac atcgtgcgct cgttatccaa tttggcggta
     2581 acggttacgg taacgtccac gtcgtagtag ccgtcttcca gcttttggct gccggtggaa
     2641 acgcgcatat ccacttcggg atcgccctgt tccaaaaaga tttgcggcgc gtgcggcact
     2701 tccaaagaca agtctttgac atacagtcgc tcgatgctga atacgggttg cagttcttcg
     2761 ctcattttgt tttcctagtt gtgggttaag ggttcagcag tccgtccagc ccgccttcct
     2821 gctgaaggcg gtagaggtcg gtaaatcctc cgacgtgcgt ttcgccgatg aaaatctgcg
     2881 gcacgctgcg ctgtcccgaa agccgctgca tttcggcaaa ggcttcgggg cttgcatcga
     2941 cacggatttc gtcgatatgt ccgacacctg ccgcgtgcag cagccttttc gccatcgcgc
     3001 agtaggggca aaacggacct gtgtacatgg taacggtctg catattgggt ttccgaaagt
     3061 tttgcaatga taatcaatat aggggcaatc gctccgtttg gcaagtgcgg aacagatgca
     3121 cgttcagacg gcatgtgcgg agtgtgtcaa agtttctttt ttaaagtatg ataggcattg
     3181 tgaaaaatat ttttgcaccc gctctgcgcg gcggggcgga acggatgcaa aaatattttt
     3241 attacatttt caggaaaaac catgttgtca ggactcccca tccccaaaga catcgcgcgc
     3301 ccgcccgaaa cgatattggt caacatcacg ccgcaggaaa cgcgcgtggc ggtgttggag
     3361 gaaaacaata tctgcgagct gcacatcgag cgcaacagcg ggcacagcct ggtcggcaat
     3421 atctatctgg gcgtggtgcg ccgcgtactg cccgggatgc agagcgcgtt tatcgacatc
     3481 ggcttggaac gcgcggcgtt tttacacatc gtcgatgtcc tcgaacaacg ccgcaacccc
     3541 gaagaaaccc agcgcatcga acatatgctg tttgaagggc agtcggtttt ggtgcaggtc
     3601 atcaaagacc cgatcaacac caaaggcgcg cggctttcca cccaaatctc gctggcgggg
     3661 cgtttcctcg tccaccttcc gcaagaagac cacatcggcg tgtcccaacg catcgaagac
     3721 gatgccgaac gcagcagcct gcgcgaacgc ctcgacaagc tcctgccgga aaatgcctgc
     3781 cggggctaca tcatccgcac caacgccgaa aacgccaccg acgaacagct ccagtccgac
     3841 atcgactacc tgaccaaagt gtgggaacac atccaagaac aggcgaaaat ccggccgccg
     3901 gaaaccctgc tttatcagga tttgccttta agcctgcgcg tgttgcgcga tatggtcggc
     3961 tgcgacacgc agaaaatcct cgtcgattcc accgtaaacc acgggcgcat gacgcgtttt
     4021 gccgaacaat acgtccacgg cgcattgggc aggatagaat tgttcaaagg cgaacgcccg
     4081 ctgtttgaaa cccacaacat cgaacaggaa atcagccgcg ccctgcaacc gcgcgtcaac
     4141 ctcaacttcg gcagctacct gattatcgag tccaccgaag ccatgaccac catcgatgtc
     4201 aacaccggcg gcttcgtcgg cgcgcgcaat ttcgacgaaa ccatcttccg caccaacctc
     4261 gaagcctgcc acaccatcgc ccgcgaatta aggctgcgca accttggcgg catcatcatc
     4321 atcgacttca tcgatatggc gcaggaaagc caccgcgaag ccgtgttgca ggagcttgcc
     4381 aaagccctcg ccttcgaccg tacccgcgtt accctgcacg gttttaccag cctggggctg
     4441 gtcgagctga cgcgcaaacg ctcgcgcgaa aacttaaacc aagtcctctg cgaaccctgc
     4501 ccttcctgcc aaggcagggg ccgcctgaaa acgccgcaaa ccgtgtgcta cgaaatccag
     4561 cgcgaaatcg tccgcgaagc gcgccgttac gatgccgaaa gtttccgcat cctcgccgcc
     4621 cccaacgtca tcgatttgtt tttggacgaa gaatcgcaat ccttggcaat gctgatagat
     4681 ttcatcggca aaccgatttc tttggcggtc gaaaccgctt acacgcagga acagtacgat
     4741 attgtgctga tgtaaaaaat gccgtctgaa gccttcagac ggcatctgtc tatttcaggg
     4801 tttccttgtc caacagcgcg cgtatcaaca gcccgtgtcc gaaacgtcgg ctgtcggtca
     4861 gttccaaata tccgccgtat tttttggcca gcgtgtcggc aatcgacagc cccagccccg
     4921 ccccctgctg ctccgttccc aaaatacggt aaaacggatc gaggacgcgg gcgcattcgg
     4981 attcgggaat gccgttcccg ttatcctcca cccacacggc gagatatttc ccttcgtccg
     5041 tgaaacccaa atctatcctg cccccgttcg gcgtgtaacg taccgcgttg tcggcaaagg
     5101 ttttaaccag cgtatagatt tccgtttcgt cggcagacac ttcgacatcg cctccgaccg
     5161 ccacaccgat gtcctgacgt ttttccaaaa ccagcggcat cagttcctgc aacacttggc
     5221 ggaaacggct ttgcagaccg aatgtcgttt tcgccaaggg gggtttcgtc cgactgcgaa
     5281 cgcgccagtg ccaaaagctg ttcgagcagg tgtttgttgc gcctgatgct ctgctgcaaa
     5341 acggcggact gccgccccgc atcgggtggg agcggcatat tgttgagccg ttccgcctga
     5401 agggaaaggg cagtcatcgg cgtacgcaat tcgtgtgccg cgtcggcgac aaaccgctga
     5461 cggcggcgta tatcttcatc aacacgtttc aaaagcagat tgatggcggt tacaaaccct
     5521 ctgatttcac tgggaatatt gtacacactc aaagcaggca ggccgttgat tcggcgttgt
     5581 tcgagacttt gcgacaattt gcggacgggg cgcatggctt tgtgcgtaat ccacacagtc
     5641 agcagtatca tcagcggcag tgccgccaac aggggcaaca cgctttgccg tgccgcatcc
     5701 tccgccaaat cttcacggta ttcgttttcc tgcatgacgg caatccgtcc ctgttcggtc
     5761 gtgcggatat aggcgcggta ataatcgtcg tccccgtccg cccgaagcgt gtgcagaccg
     5821 tccgccagat gcgcgggcag gctgacaaca gggtcttcct gccgcggcat ctgtaccaaa
     5881 atacgcgtat cgccgtcgcc ctcgggcaaa gtttcgggcc tggaatcggg gccgacatac
     5941 aatgccgcct gacggagcag gtcgtcctgc aactcttccg tttcgtggaa ggtttcgtag
     6001 taggaaaaca tacctgcaag cattgccagc ggaacaaaca tccaaatcag ggcaaggctg
     6061 attctgacct gaagcgaatg ttttaaaatt ttaaaaaaac ggtccggcat cttcgttcct
     6121 gttcagacgg catcttgacg cggcatcagc cagccgacac cgcgcacatt ttgtatgctt
     6181 tccttaccca atttcttgcg cagcccgtga atcagaaaat ccaccgcatt gctttcgact
     6241 tccccgcccc aaccgtaaac cttgtcctcc gaatccgagc gcgacaaaat cacacccggc
     6301 ctcgccagca aagcctgcaa taccgaaaac tcctggttgc tcaatgccac ctgcctcccc
     6361 tcggcaataa tttctacctg atacgttgca ggattgaggg ataacgcacc atttgacaga
     6421 cacgcctgcg cctgtccgct cccgcgccgc aacaccgccc gcatccgcgc cttaagctcc
     6481 gccatatcga acggcttgac gatatagtca tccgcaccgc cgtccaaacc gttcagccgg
     6541 ctgtacaaat catcccgcgc cgtaacgatc agcacgggga cggtacagcc tgccgcgcgt
     6601 atttccgaca aaacatccaa accgtcccgc ccaggcaaac ccaaatccag cagcatcaag
     6661 tcataaggct gggcagcggc agccgccgca acctgcgcgc cgtttttgac ccaatccacc
     6721 gcatagccgc cgtctttcaa acttgccgac accgcttacg caatcatcgc atcgtcttcc
     6781 accagcaaaa cacgcatcaa ctttcccttc aaaataaacc gtgcctattc taacacccca
     6841 aaattagccg caatttagcg gcctttacgc ttgccggtat ttttcaaaac tgcggcacaa
     6901 aaaaccgcgc cggcaactgc cttcagacgg cattggggcg cgattgcaac acacggacag
     6961 gcaggcaaag cctgcgacag atcacaggaa cgattcgggc ttcaggcggc ttcgccgttt
     7021 acggcagagg cacgattcct gccgctatcg aactgatcaa tatcgccagc gacaaacccc
     7081 acgcccagaa aaacgaataa cggatgtgtt tgcccatcga caatttcgcc aaacccaaac
     7141 ccatccacaa agccggcgaa agcggcgtaa caaaagtacc gacgatactg ccgatcaaca
     7201 tcgcatagcc tgccgcttcg ggtgcaacgc ccgcctgcga ggtaatctgt tccacaatcg
     7261 gaaacagtcc gaaataataa gcgtccgtac tcaaaaccaa ctcaagcgga atacccaaca
     7321 caccgatggc aatatgcaga taaggcagca acgcgtccgg caggatatgc acaaggtctt
     7381 tggaaatcgc gtccaacatc cccgcgccct tcaaaatccc caaaaacgta cctgccgcca
     7441 aaataatgga cgccatcatc accgcgccgc cggcgtgggc ataaatccgc tccatctgtt
     7501 cctgcgggct gcggtaattc aaaagcaacg ccgccgttgc agccagcata aatacataac
     7561 ccggcgggaa gatacccgaa aaaagcaggc tcatcgccgc caaaaacagc aggacattcc
     7621 accaaaacag tttcggacgc gccaattttt gttcttcttc cgacaaaggc gccggcttta
     7681 tcaaatccgc cacggcgggc aacgcgccca actcccggac aatccgcctt ttttcacgca
     7741 cacccaaaaa cagggacagc acaaggataa acaccacacc gataatttgc accgtcaaca
     7801 aaggtttata caattcgccc acatctgcgc ccaacacgct tgcaacccgc ccgatcggcc
     7861 cgccccgcgg caaaaggttg attagccccg cgctggaagt cagcagcaaa aacagcaggt
     7921 aaggattcat atgcagacgc ttgtaaagcg gcaaaagggc agggacgacc aataaaaacg
     7981 tcgtcgcgcc cgccccgtcc aactgtgcca ccaccgacac caagaccgtc cccacactca
     8041 ctgccacgat attaccccga gtcagcttaa tcaaaccgcc tatcatcgga cggaacagcc
     8101 ccacatcgtt catgattcca aaaaacaaaa tggaaaacat aaacataatg acaatctgcg
     8161 tcaccgattt cgtgccgccc gaataaaatt cttttaattg ggatacatca aaccccgcca
     8221 gcaacgcccc aatcagcggc accaagatta atgcgatgat gggcgacact ttttccgtca
     8281 gcaacagcca tacgatgacc ccgataatca gcaatccgat aaacgtcagc attatttctc
     8341 ctttatttta ttttgaacat aaaaccgccc gtgcaggcaa aaccgccaac atatgcggga
     8401 taagccttgc attccattca cgcctgccga catttctgca tccgtccgtc atttcacggc
     8461 ggcaaccgat acggaacaac cggcaaaccg gtatcgggac ggcgcggggg catccgtccc
     8521 ggtgcaccga ttcaaacgaa actgccccct atcattgcgg agcgcggggc gtgccgtaca
     8581 cgcgggattt taaacacgcc gatcatttgc cgcgcattat ccggcaaacg gcaaaccttg
     8641 acgctgcccg gcccatataa aaaagccgca aaacccgaac cggttttgcg gcttttcgat
     8701 ttggtttgtt tatttggcat ttctttcaat caaaccgaca aactgattga tatatgactg
     8761 aacaaaatcc cttgccgagt caatcaactt accgttttca tcaaacagcg tcggcgaatt
     8821 gcccaaaaat acttccggct gtccggttac gggcatatca aaatacgaca gggcaaggcg
     8881 caggtttttt tgggaactgt aaccgcccat cttgccgacg gaatggctga tgatgccggc
     8941 cggtttgttt ttccaagcca cgtcggcatt cggtttcgag ccgatgtcca ccgcattttt
     9001 caaacaggcg ggaatggtgc ggttgttttc ggacgtaacg aacaaaatgc cgtccgaagc
     9061 cttaatcgtt tcgcggaaag ccgtgtagct ttcgggcagc ggcacatctt ccaccgcagg
     9121 gtcgtcataa tcgaaattgt aaagcggcag atgtccgatt tcgacgattt ccgcctgcca
     9181 gccttcgggg aacatctccg ccgcattcaa tgccactttg cgcgcaaacg aagcacggcg
     9241 caggctgccc accaaaatac tgattttctt agccacaatc attcctcctg aatattaagt
     9301 ttgtgcgttt caatcatttc ataatggtaa cgattattat atatgtgatt tcccctgcaa
     9361 acaagccggt ccgccgcccc ggcgttccca cttatccggc tttgccttat aattgctttt
     9421 tttatgtaac agattcacct atgaatttcc ccaaaacagc ggcctccctg ctgctgcttc
     9481 tcgcctccct cgccgcacac gcgctcgata ccggccgcat tccgcaaaac gaaatcgctg
     9541 tatatgtcca agagcttgac agcggaaaag tcatcattga ccaccgtgcc ggcatacccg
     9601 tcaatcccgc gtccacgatg aagctcgtta ccgcgtttgc cgccttcaaa accttcggca
     9661 gcaattaccg ctgggcgacc gagtttaaaa gcaacggtac ggtaaacgac ggcacgcttg
     9721 acggaaacct gtattgggcg ggcagcggcg accccgtttt caatcaggaa aacctgcttg
     9781 ccgtccaacg ccagttgcgc gacaaaggca tccgcaatat cacggggcgc ctgatgctcg
     9841 accacagcct gtggggcgaa gtcggcagtc ccgaccattt tgaagccgac agcggttcgc
     9901 cgtttatgac gcccccaaat ccgactatgc tgtctgccgg tatggttatg gtgcgcgccg
     9961 aacgcaatgc cgccggcagt accgacatcc tcaccgatcc gcctttgccg catatttttg
    10021 cccaaaacaa cttgaaaatt accgcctccc aagctgcctg cccttcggtc aaaaaactga
    10081 tgcgcgcatc tttttcgggc aatacgctga aattgcgcgg caatattccc gaaagctgtt
    10141 tgggcaagcc tgtcggtgtc cggatgttcg cgcttgacga actgatccgc caaagtttta
    10201 ccaaccactg gctgctcggc ggcggacgga tttcagacgg catcggcata gccgacacac
    10261 cggaaggcgc gcagacgctt gccgttgcac actccaaacc gatgaaggaa attttgacgg
    10321 acatgaacaa gcgttcggac aatctgattg cgcgttccgt cttcctcaaa ctcggcggcg
    10381 acggcaaact gcccgccgtt tccgaacagg cggcgtctgc cgtccggcga gaacttgccg
    10441 tatcgggcat cgatgttgcg gatttggttt tggaaaacgg ttcgggtctg tccagaaaag
    10501 aaagggtaac ggcgagaatg atggcgcaaa tgttggaaac ggcttatttc agcccgtttg
    10561 cacaagattt catcgacacg ctgcccatcg ccggcacaga cgggacttta cgcaaccgct
    10621 tcaaacaaag cggcgggctg ttgcgcttaa aaaccggcac gctcaacaat gtccgcgccc
    10681 ttgcaggtta ttggctgggc gacaaaccga tggcggtggt cgtcatcatc aacagcggcc
    10741 gcgccgtttc cctgctgccc gacttggaca acttcgttgc caaaaacatc atctccggcg
    10801 gcgacggctg gctggatgcg aaactgatgt gcaaagaacg ccgcgcctga atcgggaaaa
    10861 tataaaaatg ccgtctgaac tgttcggacg gcatttttga ttttcaaacc ggaattacag
    10921 ccccgctgcc gctttcaatg aggccgcttt gtcggtgcgc tcccaagtaa actcaggttc
    10981 ttcgcggccg aaatgtccgt aggcggcaga ttttccgtaa atcgggcgca agaggtcgag
    11041 catttggacg atgcctttgg gacgcaggtc gaaatgttcg caaaccagcg caatcagttt
    11101 ttcctcgctg attttacccg taccgaaagt atcgatggaa atcgaagtcg gttcggcaac
    11161 gccgatggcg taggaaactt ggatttggca ttgggttgcc aagcctgcgg cgacgatgtt
    11221 tttcgccaca taacggcagg cgtaagcggc ggaacggtcc actttggacg ggtctttgcc
    11281 ggagaatgcg ccgccgccgt gcggagccgc gccgccgtag gtatcgacaa tgattttgcg
    11341 gccggtcaaa ccgcagtcgc cttgcgggcc gccgatgacg aagcggccgg tcgggttgat
    11401 caggtatttt gtttcgtcgg tcagcagttc gggcggcaaa acgggcttga taatctgctc
    11461 aatcacggct ttactcagtt cttcatggct gatggcagga tcgtgctggg tggacaggac
    11521 gacggtatca atgcgtttca ccttgccggt ttcgctgtcg taaaccacgg tcagttgggc
    11581 ttttgcgtca gggcgcagcc aaggcaggcg gccgtctttg cgcaattcgc tttgacgctg
    11641 catcaggcgg tggctgtaat agatggcaaa cggcatcagg gtcggggttt cgtcgcaggc
    11701 atagccgaac atcaagcctt ggtcgcccgc gccttggttc aggtcgatgc cttcgccttc
    11761 gttcacgcct tgggcgatgt cgggggattg ttggtcgtag tacacgccga ccgcgcagcc
    11821 gttggcgtca aagcccggct cggaggagtt gtagccgatg cgtttgatgg tttcgcgtgc
    11881 gactttgatg tagtccactt gggcggtggt ggtgatttcg cccgccaata cgcacaagcc
    11941 tgtgttgacc aaagtttccg ccgcgacacg cgctttgggg tcttgcgcca agatggcatc
    12001 caaaatcgca tcggatactt ggtcggctac tttgtccgga tggccttcgg aaaccgattc
    12061 ggaagtaaac agatattcgc tcattgaaat tttccttgaa aaacaaacca tcttcttcag
    12121 acagcatgtt gtatgaacat aatgtcgaca gcgggaaata tagcaaaatt tccctattcg
    12181 tgccattcag ttgagaaata ttcctattta aacggcgctt tggaatctct gtccgtacgt
    12241 ttcgtacagg caaaaaaatt cccgcatcaa gcgggtttgg attgctccgg cgagccacat
    12301 cggtttttca accgtccacc ttacctttcc gtttgaaaag caggttggca tggaattccc
    12361 aactcttaat gcagcgcgca tcgtagcaga aaaggcatat tgccgcaata cttccctttt
    12421 tcagacggca tgtttcgttt acaattcagg ctgtttcccc ctttgcgaac cgccatgcac
    12481 atcctgttga ccgccctgct caaatgcctc tccctgctgt cgctttcctg tctgcacacg
    12541 ctgggaaacc ggctcggaca tctggcgttt taccttttaa aggaagaccg cgcgcgcatc
    12601 gtcgccaata tgcggcaggc gggtttgaac cccgacacgc agacggtcaa agccgttttt
    12661 gcggaaacgg caaaatgcgg tttggaactt gcccccgcgt ttttcaaaaa accggaagac
    12721 atcgaaacaa tgttcaaagc ggtacacggc tgggaacacg tgcagcaggc tttggacaag
    12781 ggcgaagggc tgctgttcat cacgccgcac atcggcagct acgatttggg cggacgctac
    12841 atcagccagc agcttccgtt ccacctgacc gccatgtaca agccgccgaa aatcaaagcg
    12901 atagacaaaa tcatgcaggc gggcagggtg cgcggcaaag gcaaaaccgc gcccaccggc
    12961 atacaagggg tcaaacaaat catcaaggcc ctgcgcgcgg gcgaggcaac catcatcctg
    13021 cccgaccacg tcccttctcc gcaggaaggc ggcggcgtgt gggcggattt tttcggcaaa
    13081 cctgcataca ccatgacact ggcggcaaaa ttggcacacg tcaaaggcgt gaaaaccctg
    13141 tttttctgct gcgaacgcct gcccgacgga caaggcttcg tgttgcacat ccgccccgtc
    13201 caaggggaat tgaacggcaa caaagcccac gatgccgccg tgttcaaccg caataccgaa
    13261 tattggatac gccgttttcc gacgcagtat ctgtttatgt acaaccgcta taaaacgccg
    13321 taacgaaaat aaaaatgccg tctgaacagg tttcagacgg catcttgtca tttgacgatt
    13381 tcggacaacg accaacgcgg acggacgttg aacgcaccga cctccctgcc cttgcccagg
    13441 tgcatcgcac cggcaaaggc aatcatggca ccgttgtccg tgcagtatgc catcggcggg
    13501 aaaaacacgc tgactttttc ggacggatgt ttcggcttgc ctttgggggt cgggatttgt
    13561 accgtcatgt tgccgaaagt ttcacggagt ttgcggtttg caccgacccc gccggcgacc
    13621 actacggttc tgaaccctgt ctgcaacagg gcttttttca cttttgcctc caacacttct
    13681 actaccgcat cttgaaacgc acggcaaata ttgttgcggg tttgctcggg tatggtttcc
    13741 gaaccgtttg cctcacgcac tttttcaacg gcagtcaaaa cagcggtttt caatcccgaa
    13801 aaactcatct gcaaatcgtc ggaatgaatc atcgggcgcg gaaaaacaaa cgcttcgggc
    13861 ctgcccgatt ccgcaagttc cgacagtttc gcaccgcccg gatacggcaa acccagcagt
    13921 ttcgccgttt tgtcgaatgc ctcgcccgcc gcatcatcga cgctctcgcc caaaagctcg
    13981 tagtcgccta tgcccctgac cgccataatc tgcgtatgcc cgcccgaaac caacagcgcg
    14041 acaaaaggaa agtcgggttt ttcctccgcc aacagcggcg acagcagatg tccttccaaa
    14101 tgatggacgg ggatgacggg cttatccaac gctaaagcca gcgcgttggc gtagctcgaa
    14161 cccgccagca gcgcaccgcc caaaccgggc ccctgcgtaa aggcaaccgc gtcaatatcg
    14221 ccatacgatg cgcctgcctg cgccaaacag ccttcagtca acggaacaag gcggcggata
    14281 tggtcgcggc tcgccaattc cggcacgacc ccgccgtatt ccgcgtgcat tgccatttga
    14341 gtgtgcaggc agtgcgaccg caatcctcgt tcggtgtcgt acagcgcaac gccggtttcg
    14401 tcgcaagaag attcgattcc taataccaac atggtctgat gccgttaaaa actgaaaaac
    14461 gtattctagc gaatttcggc acgactgccg tatccccaaa acggaacatg ccgtctgaag
    14521 cccttcagac ggcatcgtcg caccgtatca aagcgttccg taagaatgca gcccgctcaa
    14581 aaacatattc acgccgataa aggcgaatgc ggttacgaac aaaccgataa tcgcccacca
    14641 cgccagcact ttgccgcgcc agcccgccac cagccgcaaa tgcagccaca cggcgtaatt
    14701 caaccaaacg atgaacgccc aagtctcttt cggatcccag ctccaatagc gtccccaagc
    14761 gtccgccgcc cacaacgcgc ccaaaatggt ggcaatggta aagaacaaaa agccgacggc
    14821 aatcgcctta tacatcacct cgtcgatcaa tgccgacggc ggcagccaca gttttccgcc
    14881 tttttcttcc gcacgcaggg aaaccagttc ggcaataccg agcatcgcgg aaatgcagaa
    14941 cgcgccgtaa ccgataaagt ttgccggaac gtggattttc atccaccagg actggagcgc
    15001 gggaatcagc ggctggatgg tatgagcctc acgcgacacg ctgtaccaca acacaaagcc
    15061 gaccacgacc gccataaagc cgaacacgaa gccgcccaat ttctgcacgg caaatttacc
    15121 ctcgtaatac aaatacatca gcgcggtaat gaccaaaaac agaatgaaca cttcataaag
    15181 gttggacacg gggatatgcc ctgcatcggg acggagcaga tagctttcgt gccaacgtac
    15241 cagcagaccg gtaaagcctg ctacggcaga cacccatgca aacacggttc ccatacccaa
    15301 cagcgtgttg gtcggcacat tttttacgct tgccaaaacc gcgcccgaaa tataggcgaa
    15361 cagggcgaag aagacaaaag cacactgcca catgatcgcc gattggctgc tgaggaaata
    15421 ccgcaacagg aaaatctctg ccgatttaat gttgccgcca tacagaccga cggcggcata
    15481 ggcaagcaat acgcttaaag gaacaaacca gcgcatcggt ttgaaaaacc aacccaaaaa
    15541 cacggcaata ccggcactcg cccacaacat gaccgtttcg taaatgtcca tatgcatacc
    15601 ggaacgggtc tgcacgaaaa ccgtagccgc aaaaaccagc acggcaaata cccaatccca
    15661 aagattcaga ttgcggatca aagatttctg aatcagcagc tcgtgttccg gaagggtttt
    15721 atagtgttca gtcatgattc aagtccttgc cgagccgttg caggctctcg acgtgttttg
    15781 gaaattcctt ctgcaaatcc cgttcgctgc gggccgaaga catagcaaaa cggattttgc
    15841 cgtctgaaaa caatacccac gcccgttttt cgcgcacata aaacataaat accgtaccca
    15901 aaaccaacaa taccgagccg agatagacca aaagcgcacc cggcgaacgg gtcatctgca
    15961 aacctgagga acgcacctcg gaaaacccgt caagctggag cagcataggc gcgggatatt
    16021 ccgtcagccc cgtataggca tccatactgt gcagcaggaa acggttccgc gcttcatcct
    16081 gccgccattc gggcaagccg taccggcgta tggtttcatc caaagcagcg ttcatcacgc
    16141 cgtaaagcat ttcgtagaaa tagccctgca tcttatcctg ctgccctttc gggatattgg
    16201 acgtaataaa ttcgtccaat cccaaatagc ctttttgcgc aaagatattc agcgtgtttt
    16261 ccgcagccag catgaattgt tcgcggattt cggcaggtgc gtctttggtt gcgccggcaa
    16321 ccagacgttt gcgcccttcc ccatctttca aaaactcacg caatgccata aaggtgtccg
    16381 ctttcaactg cttgtccaag gggatacgca gccagcggta ttgctgctgc aagccgctgc
    16441 gcgtgccggt cagccaaaaa taatctttgt cctgcaaaat cggcagcata tagtttttat
    16501 attcgaccgc ctgccctgcc gcatcacgga tgcggtacac gatggaaggg ccgatattgg
    16561 tgtatttttt accttcctga gtaacggcgc ggacatcgtt cagagtggat ttcaggcttt
    16621 tttcccgttc cgcaccctcg ctcatgtcct ccacattcat agaagtgaac tgatcgaact
    16681 caagacgata tttgtgtttg ccgatttcca acggaaactg gtgtatggag gttgccttca
    16741 acacgacagg ttcgcgcgaa gcatccctca aattccacgc cttgaatgtc aaatccgaac
    16801 cgccgtcggc aaaactcgcc tgataaatcg tgatgccgtg caaggtcaaa ggatggttca
    16861 cgcggatggt gcgctcgagt ttctcaccgg ttgccttgtc cgttacttca atatcgctgg
    16921 caaaatcgcg cggcataccc gtattgtaaa aatcgatatg gaattttttc agtttgactt
    16981 caaaaggcaa gtcctgaacc aacatcccgt tgtcggcatt caggaaaacc acatccgcac
    17041 tttgcccctc ggaaatattg acgttgcccc taaatgagag attggacgca cccaaaatac
    17101 tttcgggctt gaaatccttg gcataaaccg cctgattgtc cggaacaatc cgaccggcca
    17161 gcatacccag cttcagcagc aggttactgt ctatcaaccc gcccaggcaa atgacaatca
    17221 aagcaacatg ggcaaagata tagccccatt tgttcatcgt gccttttttg gcggcaatca
    17281 gaaccgaccc gtcctcacgg ctgacggttt ttccctgaaa accccgcacc tccagataac
    17341 gtttggcaac ttcgggggca atttttacat ccaacagcga agaatggcgc atcgccgcca
    17401 gagatttttc tttaaccttt tcccggaaag acttcatttc gcgccaaaac ggcggaacgt
    17461 tacggattaa acacaaactg gtagaaacca ccagaaacat catgataacg acaaaccatg
    17521 ccgaagcata gacatcgtac aaacccaaaa aatcaaaaat ccgagtccaa aacggtccga
    17581 atttgaccaa ataatccgtc tgcggctggt tttgctgtaa caccgtgccg ataaccgatg
    17641 caatacccag cagactgagc aaagcgaccg caaagcgcat ggagctgaaa aaagcgaacc
    17701 acggacggga aagaagtgtg ggggatatac gggatttact cattgtgtgt ttattccgcc
    17761 atcaggaata tgggaaagca gaattgggca gacagaaaac aacgtcccga ttctactgtc
    17821 ttgatgcttt ttatttcaag acaatgaaga cagcctgcat cgattccaac ggttgcgatt
    17881 gaaaaaactt atcgcaaaat tttcagaagc cgtctgaaaa ccttttcaga cggcttctga
    17941 agcagaccct tggatgatta acgcaaaccc tggataaagt tagcaactgc ttttaaatct
    18001 tcttcggaca tacggtttgc aatatcttcc atgatggtat ttttacgctg accggacttg
    18061 taggcattca tctgttcaac aatatatgcc tggtgctggc cgcccaaacg cggataagcc
    18121 tgaatttcgc ttccgcctcc cggcataccc gcaccgctcg gaccgtggca ggacatacac
    18181 gccggcactt ttttatcgct caaaccgccg cgatagattt tcgcgcccaa ttcgggattt
    18241 tccttaggat tggcttcgcc ggatttgggc tgctgtttgg catagaatgc ggatacgttc
    18301 aaaatatcct gatcgctcaa attcattacc accggtttca tcacagctgc cgaaccgtgg
    18361 gtgcgtttgc cgtcgcggat gccgatagtt tgatgataga tgtaagcagt atgctgtgcc
    18421 gccaaacgcg gatacatcgc aatgccgctg ttaccgtctg ctgcatggca agccgcacaa
    18481 accgttgcgg caacctgttt gcctttttcc acgtctgctt tgggagatgc ggaaaccgca
    18541 ccggcagcca aaacaaaggc caataaagtc aatcgtctca tggagtgctc ctgattacag
    18601 cattggataa cgcaacaatg ctctttttta tattcaaata tgggattttt gacccgatta
    18661 aaaccgatga ttctgtaaac gtgttattct atactaaatt tacattaaat taccactgtg
    18721 tttcacataa aaccaaccgc atatttttgc tgtcggacaa acggcggcgg aaaacaagga
    18781 tatgcccatg aacctttttc aaaacgccaa attcttcacg acggtaaacc atctcaaaga
    18841 cctgccggac acaccgctcg aaattgcctt tgtcggcagg agcaatgccg gaaaatccag
    18901 tgccatcaat accctgacca accatgtccg tcttgcctac gtttcaaaaa caccgggacg
    18961 gacacagcat atcaacttct tcgagctgca gaacggcaat tttatggtcg atttgcccgg
    19021 ctacggttat gcccaagtcc ccgaagcggt acgcgcacat tgggtcaatc tgctcggcga
    19081 ctacctccgc catcggaaac agcttatcgg gctggttttg attatggatg cccgccatcc
    19141 tttaaaagaa ctcgacatcc gtatgctgga ctttttccac acgaccggca gaccggttca
    19201 catcctgctg tcaaaagccg acaaattatc caaaaacgaa cagataaaaa ccctgtccca
    19261 agtcaaaaaa ctgctcaaac cttattccga caggcaaaac atcagcgtac agctgttttc
    19321 cagcctgaaa aaacaaggta ttgacgaggc caaccgaact gtcggaagct ggtttgacgc
    19381 agcagatgcc gccgcttcct ctccagagga aaactgacct caattatacg gaaaccgtat
    19441 ccccccactt gaccggccgc aaacatttaa aaaattgcca ctgccaaatc taaaatgccg
    19501 tctgaaagac ttttcagacg gcattttgcg gagtctttaa aacagggaat ccaactgctg
    19561 ctgtttggaa tccgtattgc tcggaagcac cggcgtttcc tgtacgtcct gacgcgtttc
    19621 gtcagacctt ccctgctgtt cgttttctac tgccgcttca tcatcttctt ttgcccgtcg
    19681 ggaaggttgc ggcgcaatac cgctgttgtc cagcatcaag cccggatcgg ttaccatacg
    19741 ttccttcata tagtattcgc cattgctgct gaccacacct tcaggcattt tcatcccttt
    19801 gccctgcttt cctttcaacg caaaacgcat atagtccacc caaaccggca ccgcaatcgt
    19861 accgccgtag ccggcacgcc ccatactctt aggtttgtcg aagccgatat atacggcagt
    19921 aaccacatca gggttaaaac cgacaaacca cgcatcttta ttgtcgttgg tcgtacccgt
    19981 tttaccggca atatccgttc ttcccaacgc agctgccccc cttgccgtac cgacacggac
    20041 cacatcctgc ataatcttat acataatata ggcattgcgc ggatcgattg cctgaggcgc
    20101 attttgccct gccaccaaag gttgcatttg ggcgcgcaac ctgccgtctc tgtcataaat
    20161 cttatcgatc acgtgcgaag aaaccctata tccaccgttc gcaaatacac tatatgcctc
    20221 cgccactttc aacggcgtcg tctcgcccgt acctaaagcc atagacaggc ttgccggcag
    20281 ctcggacggc ctgaagccga aacgccggat atactgttgc gcgtaaccga caccgataga
    20341 catcaaaata cggatggaaa ccatattctt ggaagccgtc agagcctgtc tcaaagtaat
    20401 gtagccggaa tatctgccgt ctgaattttt aggtgtccaa accgaaccgt tcggcccttt
    20461 ccccggcagg gaaatcggcg catcgttaac cactgtggac gcggtcatcc ccttagataa
    20521 tgccgccgaa tagacaaacg gcttaaaggt cgaacccggc tgccgcattg cctgaacggc
    20581 acgattgaat gttttgctgt gaaaatcata accgccgacc agcgcgcgca cagctccggt
    20641 ttttgcatcc agcgaaacca aagccccctg cagcaacggc tcttgaacca ccgcccaacg
    20701 cccgccgttg tttttgacac ggatgaccgc gcccctgcgg atacggtcct cccccatttt
    20761 ctcattatcg accgctcggg ccgcaaaacc caaggcgcgc ctgtcaagcg caacccgcct
    20821 gccgccgggc agctgtatga cgacattttt ctttttagta acatccaaca caacggcgga
    20881 aaccatttta tcgacggtat agagtcccga cagatactgg ctgacagtct cctcgacatc
    20941 ttcactctta ctcaaatcga tatagttttc cgcaccgcgg tagctgctgc cgcgatcgaa
    21001 attccgtaga gccttgcgca atgcctcggt tgccgccttc tgatgatcgg tgcggaccgt
    21061 ggtataaacc ttaaaaccct gcgtataggc atcttcacca tatttctcat acagttcccg
    21121 acgcaccatt tccgccacat ataaagcact ctgatcgatt ttccgaacaa accgctcata
    21181 atgcagttcc tcattcaatg cctgatcgcg ctgttgcacg gtaatcatct tctcctcgag
    21241 catattgttc aaaatatact tctggcgcaa cttggcacgc tccggattaa caatcggatt
    21301 ataggcagac ggagccttgg gcagtcccgc aagcatggcg gcttccgcca aagtcaaatc
    21361 tcggacattc ttattgaaat agatttgcgc ggcagatgca aaaccatagg cacgctgacc
    21421 gaggtaaatc tgattgaaat acaactcaag gattttgtct ttgcttaaag actgctcgat
    21481 tttataggca agcaacacct cattgaattt gcgtgtgaac gttttttcac tgctcaaata
    21541 aaaatttttc gccacctgct gtgtaatcgt actcgcaccc gactgcacgc tgccggacac
    21601 gacattgccg acggcagcgc gggcaacacc ccaaacatcc accccccaat gccggtaaaa
    21661 gcgtttatcc tcggcggcaa taaccgcatt ccgcaacacc tcggggaaat cgccgatttt
    21721 tgtaaattcg cgccgctgct ccccatacat accgatgact tctccatccg ccgaataaat
    21781 agtcaacggc attttaggct ggtaatgctg caaagaatcc aaagacggca gtttcggata
    21841 cgttaccaaa atagcaatgg caaccagacc cactccaaat acacaaaaac caaaaaacaa
    21901 accaaaacaa gtagttaaaa tctttttaat catagctgaa taataattta ccattattgg
    21961 tattaaataa agtaaaatag caaccgattt ctacaaagca cggtttcaat gtgcaaagaa
    22021 caaggaatcc attacggata ccgaaacggt tactcactgt acaaataaag caggaacttt
    22081 catcatgcgc ttgtttaaaa gcttgaaaaa ccctaaaaaa acagatgcca agctccctaa
    22141 aaaatcttcg ggactcaata accgcgcggc aatcggcatc gatatcgacc agcattccat
    22201 caaaatggta caattgtcag gacgtagttt aaaccaaatt caattggaaa aatacgtcat
    22261 tgccaaatta ccaaagaata tcattcaagg caataaagtc caaaattacg atcaacttgt
    22321 tacatatttg caacaagcct atgccaaact gggtacttcg tgcaaaaaca tcgtcgcgtc
    22381 cgtcccgcaa aatctggcaa ccatcgaaca attgacctac acggccaaag atgcagaatt
    22441 ggacctgcag gggttcgtgg agtcctccat ctccgaagcc agctcgatat cgctcgaaga
    22501 agccaattac gactatcagg tcttgtccca atcggccgtc ggcgaagctg ttttgtccgt
    22561 cgcatcgaga aaggatgaaa tcgaacccct gattgacgca ttcaacgcag ccggtatgaa
    22621 attatccgcg cttgatgtgg acattttcgg acaatacaac gcctacgcgc tgtggataaa
    22681 ccattttgcc cccgagcttg cggacgaaaa agtggccatt ttcggcgtat atgccgcaca
    22741 gacctacgcc ctggtcatcc aagacggaaa aatcctatac aaacaggaaa cctccgtcag
    22801 cgaagaacag ctcaaccaac tcatccagcg cacctatcag gtaacagcag aaaaagcgga
    22861 agagatcatc aactccccgc aaaaaccttc cgattaccaa gaaagcgtgg caaactattt
    22921 caaccagcag attacccaag aaatacaaag ggtcttgcag ttttattaca ccacgcagac
    22981 cgcagacgat atgaccgaca tcaagcatat cctgctgacg ggggaagcgg tgcgccagaa
    23041 aggcatcgcc caaaccgtcg cctcgcaaac caatgcggat gtacaatgcg tccatcccgc
    23101 gcgttatttt gcgaacgacc tcaaaacaga cgaacaacaa ttcgaacttg atgcgccgac
    23161 actgaccaag gcgttcggtt tggcggtacg gggattataa ttatgaacaa tttaatcaaa
    23221 atcaacctcc tcccctacag ggaagagatg aacaaacgca aacagcagca gtttaaaacg
    23281 ctgatgtacg gcgccgtgct gacgggcgtt gccgccgttg cggcaaccta cctgtttatc
    23341 gacaatatga tcaataaaca gtcggaaaaa aacacgctgc tggaaatctc catcgcacac
    23401 ttggataccg agctgtcgaa aatacaaaag ctcaaacagg aaaaagatgc cttcctgatt
    23461 aagaaaaaca aaatcgggga gctccagatc aaacgcctcc aagccgcaaa aatcctcgac
    23521 agcctgaatg aggccgtccc cggaagcacc tacctaacct cgctggatgc cgttaccgcc
    23581 gactcttatc ggctcagcgg caggacatcc agcgacaacc gcgttgccgc catgatgagg
    23641 gcgatgccca ataccggcat attcaagcaa cccgaattgt taagcatcaa gaaaaacaat
    23701 tcgcatcaag aatttaccct tcaggcaaca ttacaaccta tcgtaaaggc ggccgaatcc
    23761 aaagagaatc cggcttcggg aaacgcacag gaggcaaact gaatggcttc taaatcatct
    23821 aaaaccaact tggatctcaa caaccttcac ctgctcaacc ttcctgccag gctttttatc
    23881 gccctgctgg tcgttgccgc cgtgctgggg ctcggttatg ccggattgtt caaaagccag
    23941 atggaatccc ttgaggaata tgaagcaaaa gaaaccgaac tgaaaaacac ctacaaacag
    24001 aaaagtatcg acgcggccag cctgaacaac cttagggacg aacttgcctc aatccgctct
    24061 gccttcgata tcatgttgaa acagctgccg acagatgcag aaattcccaa tttggttcaa
    24121 gagcttcatc aggcgggttc gagcaacggt ctgcgcttgg acagcgttat gccccaacct
    24181 cccgtagatg acggtcccat caaaaaatta ccctattcca tttccattac cggaaattac
    24241 gaacagatca gccaatttac ccgcgatgtc ggcagtctct cccgaatcat tacccttgag
    24301 tcgctgaaaa tcgcccaatc tccggaaaac ggcggcaatc ctgacggcaa gagcagtatc
    24361 ctgaacctca gcgccattgc caccacctac caagcaaaat ccgtagaaga gcttgccgca
    24421 gaagcggcac aaaatgccga gcaaaaataa cttacgttag ggaaaccatg aaacactatg
    24481 ccttactcat cagctttctg gctctctccg cgtgttccca aagttctgaa gacctaaacg
    24541 aatggatggc acaaacgcga cgcgaagcca aagcagaaat catacctttc caagcaccta
    24601 ccctgccggt tgcgccggta tacagcccgc cgcagcttac agggccgaac gcattcgact
    24661 tccgccgcat ggaaaccgcc aaaaaagggg aaaatgcccc cgacaccaag cgtattaaag
    24721 aaacgttgga aaaattcagt ttggaaaata tgcgttatgt cggcattttg aagtccggac
    24781 agaaagtctc cggcttcatc gaggctgaag gttatgtcta cactgtcggt gtcggcaact
    24841 atttgggaca aaactacggt agaatcgaaa gcattaccga cgacagcatc atcctgaacg
    24901 agctgataga agacagcacg ggcaactggg tttcccgtaa agcagaactg ctgttgaatt
    24961 cttccgacaa aaacaccgaa caagcggcag cacctgccgc agaacaaaat taagaagagg
    25021 attactccat tatgaatacc aaactgacaa aaatcatttc cggtctcttt gtcgcaaccg
    25081 ccgcctttca gacggcatcg gcaggaaaca ttacagacat caaagtttcc tccctgccca
    25141 acaaacagaa aatcgtcaaa gtcagctttg acaaagagat tgtcaacccg accggcttcg
    25201 taacctcctc accggcccgc atcgccttgg actttgaaca aaccggcatt tccatggatc
    25261 aacaggtact cgaatatgcc gatcctctgt tgagcaaaat cagtgccgca caaaacagca
    25321 gccgtgcgcg tctggttctg aatttgaaca aaccgggcca atacaatacc gaagtacgcg
    25381 ggaacaaagt ttggatattc attaacgaat cggacgatac cgtgtccgcc cccgcccgcc
    25441 cagccgtaaa agccgcgcct gccgcaccgt ttaccgagtc cgtagtatcc gtatccgcac
    25501 cgttcagccc ggcaaaacaa caggcagcgg catcggcaaa acaacaggcg gcgacaccgg
    25561 caaaacaaac caatatcgat ttccgcaaag acggcaaaaa tgccggcatt atcgaattgg
    25621 cggcattggg ctttgccggg cagcccgaca tcagccaaca gcacgaccac atcatcgtta
    25681 cgctgaaaaa ccataccctg ccgaccgcgc tccaacgcag tttggatgtg gcagacttca
    25741 aaacaccggt tcaaaaggtt acgctgaaac gcctcaataa cgacacccag ctgattatca
    25801 caacaaccgg caactgggaa ctcgtcaaca aatccgccgc gcccggatac tttaccttcc
    25861 aagtcctgcc gaaaaaacaa aacctcgagt caggcggcgt gaacaatgcg cccaaaacct
    25921 tcacaggccg gaaaatctcc cttgacttcc aagatgtcga aatccgcacc atcctgcaga
    25981 ttttggcaaa agaatccggg atgaacattg ttgccagcga ctccgtcaac ggcaaaatga
    26041 ccctctccct caaagatgtg ccttgggatc aggctttgga tttggttatg caggcgcgca
    26101 acctcgatat gcgccagcaa gggaacatcg tcaacatcgc gccccgcgac gagctgcttg
    26161 ccaaagacaa agccttctta caagcggaaa aagacattgc cgatctgggc gcgctgtatt
    26221 cccaaaactt ccaattgaaa tacaaaaatg tggaagaatt ccgcagcatc ctgcgtttgg
    26281 acaatgccga cacgaccgga aaccgcaaca cgcttgtcag cggcaggggc agcgtgctga
    26341 tcgatcccgc caccaacacc ctgattgtta ccgatacccg cagcgtcatc gaaaaattcc
    26401 gcaaactgat tgacgaattg gacgtacccg cgcaacaagt gatgattgag gcgcgtatcg
    26461 tcgaagcggc agacggcttc tcgcgcgatt tgggcgttaa gttcggcgcg acaggcagga
    26521 aaaaactgaa aaatgagacg agcgcattcg gctggggcgt gaactccggc ttcgggggcg
    26581 gcgataaatg ggaggcccaa accaaaatca acctgccggt tgccgctgcc gcaaacagca
    26641 tttcgctggt gcgcgcgatt tcctccggcg cgttgaattt ggaattgtcc gcatccgagt
    26701 cgctttcaaa aaccaaaacg cttgccaatc cgcgcgtgct gacccaaaac cgcaaagagg
    26761 ccaaaatcga atccggttac gaaattcctt ttaccgtaac tacagcctcg ggcggcggca
    26821 actctaccaa cacggaactc aaaaaagccg tcttggggct gaccgttacg ccgaacatca
    26881 cgcccgacgg acaaatcatc atgaccgtca aaatcaacaa agactcgcct gcacaatgtg
    26941 cttcaggcaa caacacaatc ctatgtattt cgaccaaaag cctgaatacg caggctatgg
    27001 ttgaaaacgg cggcactttg attgtcggcg gtatttatga agaaaacaac ggcaatacgc
    27061 tgaccaaagt ccccctgttg ggcgacatcc ccgttatcgg caacctcttt aaaacacgcg
    27121 ggaaaaaaac cgaccgccgc gaactgctga ttttcattac cccgaggatt atagatacgg
    27181 ccggcaacag cctgcgctat tgatgcgtca aaataagggc atatgtttta cagcatatgc
    27241 cctttcttta tgctttttgc cgcgaccgaa atgccgtcat tcccgcgcag gcgggaatcc
    27301 aacttgtccg gtttcggttg tttttcgtct cgtaactttt gagccgtcat tcccgcgaaa
    27361 tcggaaatcc agtccgttca gtttcggtca tttccgataa attcctgttg cttttcattt
    27421 ctagattccc acttttgtgg gaatgacggc ggaagggata aatcctcgca atccaaagcc
    27481 tcgtcatttc cacaaaaaac agcaacccga aacaccccgt cattcccgag caggcgggaa
    27541 tctaggtctt taaacttcgg tttttttccg ataaattctt gcctcattga aattctagat
    27601 tcccgctttc gcgggaatga cgggatatgg gtttccgtgc ggacgtgttc ggatttccgc
    27661 ctgcgcggga atgacgattc gggtattact gacgattcat cgggccgtag ggtgggcttc
    27721 agcccaccaa tttcaccgca tcaagggttt tggggaatag gcgtaactcg gcggtttcgg
    27781 tttttggcga agttggttgg aatcggcggg ctgaagcaca ccctacagtc caccctacaa
    27841 cccatcttac gcatctgcct tacgcattta tccttcgcct gccgatatgc cgtcattccc
    27901 gcacaggcgg gaatctagaa ccgcaacgcg gcaggaatct gtcggatacg gctgaaaccg
    27961 aacggact
//
LOCUS       DAXFHF010000024        27145 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXFHF010000024 DAXFHF010000000
VERSION     DAXFHF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27145
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.024"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            771..1034
                     /locus_tag="ACFIRM_000944"
     CDS             771..1034
                     /locus_tag="ACFIRM_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028598.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            1114..2562
                     /gene="gnd"
                     /locus_tag="ACFIRM_000945"
     CDS             1114..2562
                     /gene="gnd"
                     /locus_tag="ACFIRM_000945"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC3028599.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWGLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2625..3896
                     /gene="waaA"
                     /locus_tag="ACFIRM_000946"
     CDS             2625..3896
                     /gene="waaA"
                     /locus_tag="ACFIRM_000946"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC3028600.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWVGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3937..4386
                     /locus_tag="ACFIRM_000947"
     CDS             3937..4386
                     /locus_tag="ACFIRM_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC3028601.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFIHMLLIVFLAKAMFYISW"
     gene            4419..5393
                     /locus_tag="ACFIRM_000948"
     CDS             4419..5393
                     /locus_tag="ACFIRM_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC3028602.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5462..5537
                     /locus_tag="ACFIRM_000949"
     tRNA            5462..5537
                     /locus_tag="ACFIRM_000949"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5495..5497,aa:Lys,seq:ttt)
     gene            5758..7011
                     /gene="murA"
                     /locus_tag="ACFIRM_000950"
     CDS             5758..7011
                     /gene="murA"
                     /locus_tag="ACFIRM_000950"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC3028603.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            7092..8285
                     /locus_tag="ACFIRM_000951"
     CDS             7092..8285
                     /locus_tag="ACFIRM_000951"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC3028604.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGEVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMVKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDITDVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(8337..8585)
                     /locus_tag="ACFIRM_000952"
     CDS             complement(8337..8585)
                     /locus_tag="ACFIRM_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3028605.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8678..9595)
                     /gene="ftsX"
                     /locus_tag="ACFIRM_000953"
     CDS             complement(8678..9595)
                     /gene="ftsX"
                     /locus_tag="ACFIRM_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC3028606.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9592..10242)
                     /locus_tag="ACFIRM_000954"
     CDS             complement(9592..10242)
                     /locus_tag="ACFIRM_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC3028607.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10437..10919)
                     /locus_tag="ACFIRM_000955"
     CDS             complement(10437..10919)
                     /locus_tag="ACFIRM_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC3028608.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            11043..11396
                     /gene="arsC"
                     /locus_tag="ACFIRM_000956"
     CDS             11043..11396
                     /gene="arsC"
                     /locus_tag="ACFIRM_000956"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC3028609.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11393..12049
                     /locus_tag="ACFIRM_000957"
     CDS             11393..12049
                     /locus_tag="ACFIRM_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC3028610.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12165..13559
                     /gene="gltX"
                     /locus_tag="ACFIRM_000958"
     CDS             12165..13559
                     /gene="gltX"
                     /locus_tag="ACFIRM_000958"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC3028611.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILGGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHNLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13580..13986
                     /locus_tag="ACFIRM_000959"
                     /pseudo
     CDS             13580..13986
                     /locus_tag="ACFIRM_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13990..14481
                     /locus_tag="ACFIRM_000960"
     CDS             13990..14481
                     /locus_tag="ACFIRM_000960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3028612.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14757..17351)
                     /gene="mutS"
                     /locus_tag="ACFIRM_000961"
     CDS             complement(14757..17351)
                     /gene="mutS"
                     /locus_tag="ACFIRM_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC3028613.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17782..18786
                     /gene="gap"
                     /locus_tag="ACFIRM_000962"
     CDS             17782..18786
                     /gene="gap"
                     /locus_tag="ACFIRM_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3028614.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18938..19618)
                     /gene="tsaA"
                     /locus_tag="ACFIRM_000963"
     CDS             complement(18938..19618)
                     /gene="tsaA"
                     /locus_tag="ACFIRM_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC3028615.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            20078..20713
                     /locus_tag="ACFIRM_000964"
     CDS             20078..20713
                     /locus_tag="ACFIRM_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC3028616.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVDNPVDMLKPTGLSDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            20750..21718
                     /gene="waaC"
                     /locus_tag="ACFIRM_000965"
     CDS             20750..21718
                     /gene="waaC"
                     /locus_tag="ACFIRM_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC3028617.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21944..22693
                     /locus_tag="ACFIRM_000966"
     CDS             21944..22693
                     /locus_tag="ACFIRM_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC3028618.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22704..23639
                     /locus_tag="ACFIRM_000967"
     CDS             22704..23639
                     /locus_tag="ACFIRM_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC3028619.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23702..24313
                     /locus_tag="ACFIRM_000968"
     CDS             23702..24313
                     /locus_tag="ACFIRM_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC3028620.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(24365..24670)
                     /locus_tag="ACFIRM_000969"
     CDS             complement(24365..24670)
                     /locus_tag="ACFIRM_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC3028621.1"
                     /translation="MDEIQSKYREWCHLLLQLEENIRRWKYAVALIRDMDNFYTHEYQ
                     VRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSVDTLDRYTQEN"
     gene            24840..26111
                     /gene="purD"
                     /locus_tag="ACFIRM_000970"
     CDS             24840..26111
                     /gene="purD"
                     /locus_tag="ACFIRM_000970"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC3028622.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            26145..26747
                     /locus_tag="ACFIRM_000971"
     CDS             26145..26747
                     /locus_tag="ACFIRM_000971"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3028623.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26762..>27145
                     /locus_tag="ACFIRM_000972"
     CDS             26762..>27145
                     /locus_tag="ACFIRM_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028624.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTS"
BASE COUNT         6495 a         6292 c         7683 g         6675 t
ORIGIN      
        1 tccccgccgg tatgggaaaa catcaagatg gcggtataaa gcgcggtatg gcggaaaacc
       61 cgccttttca aagttttatt catattttat tccttaagtt tgccttcgcg ggacggcgcg
      121 ccgcgaacgc cggggttcgg taaaccgccc gattccgcgc ccgccgaatt gctgattgaa
      181 aagcttacct ccccatttta actttgcgca cttatcacgg taaagcgttt tttagcaaac
      241 cgccgcagat gcccgaccgg tccggattcc cgcctgcgcg ggaataacgg catcggtttg
      301 acggtattta attgaattgc ggaaattgac ggattcagtg agattggcgg gatgaagcct
      361 accctatagc ccgcctttta tagcggatta acaaaaaccg gtacggcgtt gccccgcccc
      421 ggctcaaagg gaacggttcc ctaaggtgat gaagcaccgg gcggatcggt tccgtaccat
      481 ttgtactgcc tgcggcccgc cgccttgtcc tgatctttgt taatccgcta tgcttataca
      541 caaccagcaa aaagccatct tattgaaata tcataagatt tactcaaaaa tcttattttc
      601 tccaccactt atgcacaaac tcggatacac actctgccaa tatgcagaaa aaaagacatt
      661 cgcgccgaca ccttttcatc cccacaagtt ccaaacacat ttccggctta aatatccaca
      721 gcattcccgt tcttaaaggc accggcagcc attcggtaaa gaaaagcggg atgtcgtccg
      781 cacaacaagc aaactatcca caaaacctat tccccacgaa aaacaatcca caggtatgca
      841 caaaagggaa tcctccttac cgacaccctt ttaaaacggc aattcagatg attttaaaaa
      901 taaaaaaaag ttacccacag aaaattcccc atatcaatat aatcatcgtt ttattattta
      961 aaccttatat tttatttata acccttcagg gaaacggcac ggcaaagcgt aaaggcatct
     1021 tttcaaaaaa ttaaaaagtt aaggattgtt aaacaagtgt aaatcaggta gagttgcagc
     1081 cgataaatta aatctataac ggagcttcct gcaatgaaag gcgatattgg cgtaatcggt
     1141 ttggcggtta tgggtcaaaa cctgattttg aatatgaacg attgcggatt taaggttgtc
     1201 gcctacaacc ggacaatcgg taaagtggac gaatttttaa acggtgcggc aaaagagacg
     1261 ggcattatcg gcgcgtattc cctgcaagac ttggtcgata agctggcaaa accgagaaaa
     1321 atcatgatga tggttcgcgc aggttcggtg gttgacgact ttgtcgaaca actgcttccg
     1381 ctgttggaag aaggcgacat cattatcgac ggcggcaatg ccaattaccc cgacacgaca
     1441 cggcggacac attaccttgc cggaaaaggg attttgtttg tcggcgcggg cgtatccggc
     1501 ggcgaagaag gcgcgcggcg cgggccgtcc atcatgccgg gcggggataa gcgcgcttgg
     1561 gaagcggtca agccgatttt ccaggcgatt gccgccaaaa cgccgcaggg cgaaccgtgt
     1621 tgcgactggg tcggcaagga tggcgcggga cattttgtca aaatggtgca caacggcatc
     1681 gaatacggcg atatgcagct gatttgcgaa gcgtaccagt ttatgaaaga cggtttgggg
     1741 ctgtcctacg acgaaatgca ccgcgtgttt gccgagtgga acaaaaccga gctggattct
     1801 tatctgattg aaatcacagc ggcaattttg ggctataagg acgaaggcgg cgaaccgctg
     1861 gccgagaaaa tcctcgatac ggcagggcaa aaaggcacgg gcaaatggac gggcatcaat
     1921 gccttggatt tgggcattcc gctgacgctg atttccgaag ccgtattcgc ccgctgtgtt
     1981 tcgtcgttca aagaacagcg cgtgcagacc ggcaagctgt tcgcccgaac cgccacgccc
     2041 gtcgaaggcg gcaaacaaga atgggtcgag gcgttgagac aggctttgct tgcctccaaa
     2101 atcatttcct acgcacaagg ctttatgttg atccgcgaag cgggcgaaag ttacggctgg
     2161 ggtttggact acggcaacac tgcgctgctg tggcgcgaag gctgcatcat ccgcagcgcg
     2221 tttttgggca atatccgcga cgcgtatgag gccaatcccg atttggtgtt cttgggtgcg
     2281 gatccgtatt tcaaaaatat tttggaaaac tgcctgccgg catggcgcaa ggtggttgcc
     2341 aaggcggtcg aatgcggcat ccctatgccc tgcatggctt cggcgattac gttcttggac
     2401 ggctatacga ccgaacgcct gcctgccaac ctcttgcagg cacagcgcga ctacttcggc
     2461 gcgcacactt acgagcgcac cgacaaaccg cgcggcgaat ttttccatac caactggacg
     2521 ggcaagggcg gagataccgc ttcgacaacc tacgatattt gattccgttg ccgaaatgcc
     2581 gtccgaaccg ttcggacggc atttgttttg caggaaatag aaagatgttc caatggcttt
     2641 atgatgtatt gtggctgctt gcgccgatat ggatacggcg ttatttggac aaacgctccg
     2701 gaagtgcccc ggcataccgg gcgcataggg acgagcgttt cggcaagccg catccgaatc
     2761 ccgttaccgg cgcggtttgg atacacgccg tttcggtcgg agaaacgcgt gccgcccagc
     2821 ccttgatacg cgagttgcgg cggcgttttc ccgatgcgcc gctgctgatg acgcagatga
     2881 ccccgacggg acgggaaacc gcgcaagttc tgtttcccga tgcgcaatgc cgctatcttc
     2941 cgtatgacaa aaaaacgtgg gtacggcagt ttttgcgcga acaccgcccg atgttcggca
     3001 ttttgatgga aaccgaaatc tggcccaatc tgatgaagga atgccggcga gcgggtgtgc
     3061 cgctgttttt ggcgaatgcg cggctgtcgg aaaaatcgct gaacggttat ctgaaagtcc
     3121 gccgcctgat ccgtcccgca gccgcttcgc tgacgggctg tctggcgcag acggaggcgg
     3181 atgcggcgcg gttggcgaaa ttgggcgcgg catccgtgca ggtgtgcggc aataccaaat
     3241 acgacctgat gccgtctgaa gacatgaaaa cgctggcggg gcagtttgaa aaacgcatcg
     3301 gaggcaggcc ggttgccgtg tgcggcagca cgcgcgttta tcggggcgaa gacgaggcgg
     3361 aaaaactgct ggcggcgtgg caacaatatc gcggcgatgc cctgctggcc gtcgtgccgc
     3421 gccatcccga gcattttcag acgacatttg aaacggcgaa acgcttcggg tttaaggttc
     3481 agcggcgcag cgacggtttg ccggtcgaac ccgatacgca ggtgtgggta ggcgacagta
     3541 tgggcgagct gtatgcgtat tacctgtgcg ccgatgtcgc ttttgtcggc ggcagtctgg
     3601 tcggttcggg ctgtcagaac atcatcgaac cgctttcctg cggcgttacg acgatattcg
     3661 gcttttcgac ctacaatttt tccgaagcct gccggcacgc cttggcatcg ggtgcggcgg
     3721 ttcaagtcga atcggcggac gcgtggcgtg aagccgttga aaaaaactta tctggcgagg
     3781 ggggcggaat gcagatgcag gcgcgcgtgg acggctttat cgcacaacat cgcggagcgg
     3841 gcgcgagaat cgccgaggcg gtgcgggaag cggtatgcgg acatcggggg cgatagtgat
     3901 acaatccgta tccgagtttt ccggttggag catcgtatga gtatttatgc cgtcgcgcac
     3961 atcatccacc tgtattgcgc caccgccttt gtcggcggcg tgttttttga agtgctggtt
     4021 ttgtccgtcc tgcatacggg acgggtgtcg cgcgaggcgc ggcgcgaagt ggaaaaggca
     4081 atgtcttacc gcgccgtcag ggtgatgccg tttgcggtcg gactgctgtt cgccagcggc
     4141 atcgtgatgg cggcaaaccg ctaccttcct atatcgggcg aaccgtttgc cacttccttc
     4201 ggtacgatgc tgacgctgaa aatcctgttg gcgttcagcg tgttggcgca cttcgccatc
     4261 gccgtcgtca aaatggcgcg ttccacgctg acggtcggct ggtcgaaata catacacgcc
     4321 gtcgtcttta tccatatgct gctgattgtc tttttggcaa aagcgatgtt ttatatcagc
     4381 tggtaacaca actacatttc aaatcaggag attgaaccat gacagaatat cccggcatcg
     4441 ggtcgccgtt gttttacggc gttttttttg cggcagtgct ggtcatgatt gccttggata
     4501 tgttttcgct gaagaaaaac ggcagccata aagtcggcat caaagaaacc ttggcatgga
     4561 gcggcttgtg ggttgccgta tcctgcctgt tcgcaggctg gctgtatttc gaactcgccg
     4621 gcaatcccgg ctacggcgcg gcagccgcaa aagaaaaagt attggaattc tttaccggct
     4681 acattttgga aaaatcgttg gcagtcgaca atattttcgt gttcctgatg atattcggct
     4741 acttcaaagt cgcaccgcag tttcaacacc gcgtgctgct gtacggcgta ttgggcgcat
     4801 tggtattgcg caccgtcatg attttcgtcg gcgcggcact ggttcggcag ttcgagtgga
     4861 ttctgtatct gttcggcgcg ttcctgctct ataccggcat acacatgatg aagcccgaag
     4921 gagatgagga agaggatttg gcaaacagca ggctgctgaa tgccgtcaag aaagtcgttc
     4981 cggtcggcac ggcgtttcac ggcgagaaat tttttaccgt cgagaatggc aaaaaaatcg
     5041 ccacgccgct gtttttggtg ctgattatga ttgaattgag cgatgtcgtt tttgccgtgg
     5101 acagtatccc cgccgtcttt gccgttacca ccgatccgtt tatcgtgctg acttccaata
     5161 ttttcgctat tttgggtttg cgggcgatgt atttcctgct ggcggatgtg gcggaacgct
     5221 ttatcttcct gaaatacggc ttggcattcg tgttgggttt tatcggtgtg aaaatgctgg
     5281 tgatgcattg ggtacacatc ccgatttccg tttcgctgtc ggtcgtgttc ggcgcgttgg
     5341 gtgcatcgat actgacctcg ttaatttata cgaaaaaaca gcctgataaa taaaacgccg
     5401 tctgaacgcc gacaaaatgg ttgacagcat caggcttgat gtataaactt tcaaagtttt
     5461 cgggtcgtta gctcagtcgg tagagcagcg gacttttaat ccgttggtcg agcgttcgaa
     5521 tcgctcacga cccaccaggt aaagcaaaaa agctgagttt tcaaaactcg gctttttttt
     5581 gttttccgac aatccttgtt cggaaagcgc ggcatttcgt caaattcttt ggtgcggctt
     5641 ccgtattttt cccgaaaatc ccttataatt ccgcctctgc aaccttcatc gggtgatgct
     5701 ttatgcattg tcccgagtat tgaaatatta gcaagtaccc accaggaaag aacaaacgtg
     5761 gacaaactga aaatctccgc aaacggcccg ctcaacgggg aaataacggt ctcgggcgcg
     5821 aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga catcgggtac gttgcgcctg
     5881 aaaaacgtcc ctatgctggc agatgtcgca accacgcaaa agctgcttca gggcatgggc
     5941 gcgcgcgttc tgaccgacaa tatcagcgaa tttgaaatca acggcggtac ggtcaacaat
     6001 acctgcgccc cttacgaatt ggttcggacg atgcgcgctt cgattttggt gcttggcccg
     6061 acgctggcgc gtttcggcga ggcgcaagtc agcctgccgg gcggctgcgc catcggttcg
     6121 cgccccgtca atcagcattt gaaaggcttg gaagcgatgg gggcggaaat cgccatcgaa
     6181 cacggttacg tcaaagccaa aggcaaactc aaaggtgcgc gcgtggcgat ggatgtcgtt
     6241 accgtcggcg gtacggaaaa cctgctgatg gcggcgacgc tggcggaagg tacgacggtt
     6301 ttggaaaact gcgccatcga gcctgaagtg gtcgatttgg cggaatgcct ggtcaaaatg
     6361 ggcgcgaaaa tcagcggcat cggaacatcg accatgattg tggaaggtgc gggcgagctg
     6421 tacggctgcg aacacagcgt cgtccccgac cggattgaag cggggacgtt cctgtgtgcg
     6481 gtggcgataa ccggcggcag ggtagttttg cggaatgccg ccccgaaaac gatggaagtg
     6541 gtgttggaca aactggtcga ggcaggtgcg gtgattgaag cgggcgacga ttggatcgcc
     6601 atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca cggtcgtcca tcccggcttc
     6661 cccacggata tgcaggcgca gtttatggca ttgaatgccg tggcagaggg aagctgccgc
     6721 gtggtggaaa cgatttttga aaaccgcttt atgcacgttc cggagttgaa ccggatgggg
     6781 gcgaacatta caaccgaggg caatacggca tttgtgcagg gtgtggaacg gctttccggc
     6841 gcggtcgtca aggcgacgga tttgcgcgcg tccgccagcc tagttatcgc cggtttggcg
     6901 gcgcgcggcg aaaccgtggt cgaacggatt taccacttgg atcgcggtta tgaaaatatt
     6961 gaaaaaaaac tcggcagcgt cggtgcgaac atcgagcgcg tatccggctg atcgggttgc
     7021 gatgccgtct gaaggcttca ggcggcatca attgtttctg tatcgcaaac actgattgaa
     7081 aaggactgat tatggcattt ttaaaactga ccgaacaaaa cgtgcggggc aaaaccgtcc
     7141 tcatccgcgc cgatatgaac gtgccgttca aaggcggcaa aatcagcgac gacacccgta
     7201 tccgcgcctc gctcgcgtcc gtcaaatact gcttggacaa cggcgcgtcc gttatcgtga
     7261 tgacccactt gggccgcccg accgaaggcg agttccaccc cgaagacgat gtcgcccccg
     7321 ttgccgcgca cttgggcggc ctgttgggca aagacgtgaa agtattgaac gactggcgcg
     7381 aaaacaaacc cgccctaaac gcgggcgaag tcgtgatgtt gcaaaacgtg cgtatcaaca
     7441 aaggcgagaa gaaaaacgat ttagaactgg gcaaagccta cgccgccttg tgcgacgtgt
     7501 tcgtcaatga tgcgttcggc accgcacacc gcgcccaagc ttcgaccgaa gccgttgccc
     7561 aagccgcgcc cgtcgcctgc gccggcgtat tgatggcggg cgaactcgac gctttgggca
     7621 aagccttgaa acagcccgcg cgcccgatgg tggcgattgt tgccggcagc aaagtgtcca
     7681 ccaagttgac catcctcgaa tcgttggcgg acaaagtcga tcagcttatc gtcggcggcg
     7741 gcatcgccaa cacattcctg ttggcggaag gcaaagccat cggtaaatcg ctggcggaac
     7801 atgatttggt ggaagaatcc aaaaaaatta tggtgaaaat ggcggccaaa ggcggttctg
     7861 tgccgctgcc gaccgatgtc gtcgttgcca aagcctttgc cgccgatgcc gaagcggtgg
     7921 taaaagacat caccgacgtt gccgaagacg agatgatttt ggacatcggc ccgaaatctg
     7981 ccgccgcgct tgccgatttg cttaaagccg ccggtacggt cgtttggaac gggccggtcg
     8041 gcgtgttcga gttcgatcaa ttcgcaggcg gcacgaaagc ccttgccgaa gccatcgccc
     8101 aaagcaaagc gttctcgatt gcgggcggcg gcgacacgct ggcggcgatt gccaaattcg
     8161 gcgttaccga ccaaatcggc tacatctcca ccggcggcgg cgcgttcctt gagttcttgg
     8221 aaggcaaaga gctgcctgcc gtagccgctt tggaaaaaac gcggcgagta aacggtttga
     8281 tataaaaaaa tgccgtctga aacgaaatgg tttcagacgg catttttgtg tggcggttat
     8341 tgtgctttgg cagcccattc cgccggagtg gcggcaaccg aaatggaaag tgcgtgcagt
     8401 tcgttgcttt ccagttgggc tttgagtccg tctttaatca ggcggtggcg cgcgaggcgt
     8461 gccttgcctt caaattctga tgaaacgatg acggcgaaaa aatggtgtcc gtcgccttct
     8521 acttcgatgt gttcgcattt tgccacgcct gcaatcaggg ccttgacttg ttcgggtgtc
     8581 agcatagtgg ggagtcctta ttggggaaat gcgccattat actggcaaat cgaaattccg
     8641 tctgaaaacg gcttcagacg gaatttttga cggtgtttta ttttttggct ttgaagcaga
     8701 gcaggtgttg tgtggtggca agccacgcgc cgaatacgcc caacgcgatg acgaagccga
     8761 acaccagccc gagttcgccg acgtagaaga aacgccagcc gatgttcaag ccgtagggtt
     8821 tgaagatggc atcgaccaag gggcgcacgg cagagagcag ccaaccgcaa agccctaagc
     8881 tgacggcggc ggagaagata ctctgccaca tagcctgata aaggaagggg cggcggataa
     8941 acgacgcggg cgcacccagg agtttggtga tttcgatttc ttctttgcgg ctgaggattt
     9001 gcagtcggat ggtgttgtgt gcgacaagga cgaaagccat acccagcgtc agggaaagaa
     9061 accataagat tttgcggatg aactcgttga tttggtacag cgtttgcacc cattcggtat
     9121 ccatagacgc ggattcgacc ataggcagct tggtaatgtc ccggtagatt gcctgcattt
     9181 gggcgggcgt ggttgccggg tcgggggtaa cgataaagac atccggcagg gggttgccgt
     9241 caagcatgga aatcagattt tggtcgaggt tggactgtaa ttccgccaaa ccgtcttcct
     9301 tgccgatgaa gcggatgttg tcgagccgtt tatcgcgcgt cagcaggctg cgtacggtat
     9361 cgctgtcgct ttgtgcggcg gcggtttcca tatagacggt gatttgcggc gactcgttga
     9421 gtttgcccaa cacgctttgc ccgctttgga tgcccagata cataaacagc ggcagggtca
     9481 tcgcgacggc aagcattatg agggtaagca gtgtgccgaa gggttggcgc agaagctgtt
     9541 tgagtgcgga gcgcgcggat tcgacgtgca gcgagaagta gtggatgatg ctcatgcgag
     9601 tcgtcctttc gagaggcgca ggatgcggtg tccgtagtcc gccatcaggg tttcgtcatg
     9661 tgcggcgacg atgacggtgg ttcccgcttc gtggaaggtt ttgaacaatt ccataatatc
     9721 gagcgcgtag gcacggtcga ggttggcgga gggttcgtcg gcaatcagca ggccgggctg
     9781 gtggacgacg gcgcgggcga tgcacaggcg ttgttgttca ccgcccgaga gggttacggg
     9841 atcgtccaat tctcgtcctt tcaagccgac tttttcgatg gcgatgcggg cgcgttcttc
     9901 ggctttgcgc ggcggatagc cgataatccg caggggcagg atgacgtttt gcaggacatt
     9961 gcggtcgttg aggattttgt ggtcttggaa cacgatgccg atgtgttggc gcataaagcc
    10021 gatttggttg tcggacaatg tgccgaggtc ctgcccgtta aacaggactt tgcccatgct
    10081 cggcttggtg atgcccgaaa tcagcttgag gacggtggat ttgcccgaac cggagtgtcc
    10141 cgcgataaaa atcatctcgc ctttgttgat ttggaagctg acgtttttca gggcttcaaa
    10201 accgccggga taggttttgg aaacttgttc gaaacggatc ataggaggtc ctgtaaggaa
    10261 gggcggcata tcgtttcggg cggttgcgtc cgcagggata tgccgtctga acaataggta
    10321 agacagtacc gattataaag attccgcctg tttttagata gtggataagt ttggcggata
    10381 ccgaaggccg ggcgggaaaa atgccgtctg aagcggcttc agacggcatc cggcgtttaa
    10441 cggcattttg aatgggcgag tttgacggct tcggtcaggc ttttctcgtt cacctctcct
    10501 gtaatggtct gcctgtatcc gcatttcggg gcttcgacga cggtaaaggg cagtacgccg
    10561 acattgtttc cgtaggattt cataaagttt cggctgttcg ccccggtgta acgccaaatc
    10621 gggtagctga ccggggtctg cttgagaaag ttgccgatat tgtcggatgt gtcgagcgcg
    10681 atgccgacca tatcgacgct gccttttttc tgcgctttgt accatttgga catggcgggc
    10741 atctcttttc gacacggacc gcaccaagtc gcccataggt tggcgatgcg gacgggggct
    10801 ttgagcgatt gcaggttttg cggggtattg tctttccatc cggccagttc gtctgccgag
    10861 gcggtatgtg cggcaaatgt ggcggcaagg gcgattgcgg cgagattcaa gcgtttcatc
    10921 gttttgtcct ttgtatgtcg attcgggtat tgtaaaccgt ttggtacggt tggaggcggg
    10981 gtattgagta aaaccctgcg taagccgata ataaggggat tgtcgagaag tagggaaatg
    11041 tcatgtctga aatcaaaatt ttccacaatc cgcgttgcag caaatcgcgt gccgccgtgt
    11101 ccctattgga agaacgcggc attgctgccg aagcggtcaa atatttggat acgccgcccg
    11161 acttgtccga attgaaggat attttcaaca aattgggctt ggaatcggcg cgcgggatga
    11221 tgcgcgtgaa agatgatttg tacaaggaat tgggtttgga caaccccgat ttggacaatg
    11281 acgcgctgct gcgtgccatc gccgatcatc ccgccctgtt ggagcgtccg attgttttgg
    11341 caaacggtaa ggcggctgtc ggcagaccgt tggaaaatat cgaagcggta ttgtgaacaa
    11401 acggctgttt tgcagccgca acggcttgcg ctattacctg ttgggcggtt tctgtttgag
    11461 tgtttttccg cttttgctgg tgtttgcctc atccgtttgg gcggtttatc ggacgggcgg
    11521 gcaggttttg ccgccgtatg tccgggcgga tgccgcgctg gtgttgggcg cagccgcgtg
    11581 ggacaaacgc ccttccccgg tttttcggga acgcatcaac cacgccatcg cgctttacca
    11641 aagccgcagg gtaggcaaaa tcgtgtttac gggagggcga accaaaaaag gctatatgac
    11701 cgaagccgag gtcgggcggc ggtacgcgct caaacagggg gttccggcgc gcaacatcct
    11761 gtttgaaaac acttcgcgca acacttatga aaacctgaac aacatccgtc cggttttgcg
    11821 tgccaacggt attgccagcg tggtgattgt cagcgatccc taccatttgg cgcgggcggc
    11881 ggaaatggcg gaggatttgg gtgtccgcgt ttatatgtct gccacgccga ccacgcggtt
    11941 tgatgcggga aataagaaaa aaatctttat gctgcaagag gggtatgcgt tatccctcta
    12001 ccgtttggaa aaatggggca gccgcttttt gggatggctg tctgattgaa gcggtagaaa
    12061 atgccgtctg aaacttatcc gcaggcaggg ttcggacggc atttttgatg cctaagcacg
    12121 gtaaaaacag taaaataaac gctttcaaag atttcggagc cattatgacc gtcaaaaccc
    12181 gtttcgcccc cagccccacc ggttacctgc acatcggcgg cgttcgcacc gccttgtttt
    12241 cgtgggcgtt tgcccgccat cataaaggcg aatttttatt gcgtatcgaa gacaccgact
    12301 tggcacgttc caccgccgaa tccgtcaaca tcatccttgg cggcatgaaa tgggtcggtc
    12361 tcgattacga caacgccgac aacgtggtgt accaaacccg ccgtttcgac cgttataaag
    12421 aagtcattgc cgaactcttg gcaaaaggcg atgcctacta ctgctattgc agcaaagaag
    12481 aattggaagc gatgcgcgag aaagccgaaa aagaaggcac ggcgacttac gaccgccgct
    12541 ggcggccgga agcaggcaaa accctgcccg aaattcctgc aggcgtgcaa cccgtcgtcc
    12601 gcttcaaaac gcctttggac ggcgttacca aatggacaga cttggtcaaa ggcgaaatct
    12661 ccatccccaa cgaagcccta gacgacctga ttattgcccg cgccgacggt acgccgacct
    12721 acaacttctg cgccgtggta gacgactacg acatgggcgt tacccacatt atccgcgggg
    12781 acgaccatgt gaacaacacc ccgaaacaaa tcaacatctt gaaagccatc ggcgcgaacc
    12841 tgcccgaata cgggcatctg ccgatgattc tcaacgaaca aggcaaaaaa atctccaaac
    12901 gcagcggcga taccgtcgcc attaccgatt tcggcgcaat gggcattctg cccgaagcga
    12961 tgctcaacta tttggcgcgt ttgggctggg cgcacggcga cgatgagttt ttcacgacgg
    13021 aacaattcat cgaatggttt gatttgaaag acgtttcccc gtccccaagc cgtatggatt
    13081 tgaaaaagct ctattggatc aacggcgaac acatcaaaat cacacccgac ggcaaactta
    13141 ccgaactcgt caaaccgcgc cttgcgctgc gcgatattca tgaaaccgaa aaacctgcgt
    13201 tggaagacgt gttggcactg gtcaaagacc gcgcccaaga tttgaacgcc ttggcagacg
    13261 aatgcctgta tttctacaaa aaacaagtcc ctgccgaagc cgatgttgcc aaacactggg
    13321 acgacgaagc cgccgcccgt atgctgcgct tcgccgaacg cctcgaaggg ctggaagact
    13381 ggaacgccaa agccattcac aacctcttca aacctttctg cgacgaagaa ggcatcaaaa
    13441 tgggcaaact cggtatgccc ctgcgccttg ctgtctgcgg tacggcaaaa accccgagcg
    13501 tcgatgccgt gttggcatta atcagcaaag aggaagtgtt gaaacgcatc cgtgcttaag
    13561 ttatccacag gtgcgggcaa tgccgtctga tgtcggaatg cggcttcaga cggcattcag
    13621 acggcattca gacggaagtt gaacatgaaa aaaatattgt attttctgat gtttgttttt
    13681 tctacaagcg tatgggcagg gggtgcggaa gacaatttgc tcagcatcca atccggttac
    13741 cgtgctttac tgcaaaagca aaacaatctg gacggaaaaa tcatcgggat gcagtcggat
    13801 ttggaagatg cgcgccggcg tttgcaggcg gctcaggcag acatcgcccg tttggaagcg
    13861 gaaattcctg cagcaatggc ccaaaaagcc cggcaggctg aagagatgag gcaaatcgga
    13921 gtgcgtttgg accatgcttg gaatgcggtt tacggcgcag gggaacgaaa gcgtcggggg
    13981 aattgaccga tgaattccct ctttgtggac aacactgttt ttattacacg gctgaaagcc
    14041 gggcatatcg gcaggttggt tcaggcgttg tttgaggagt ggcacggatt tgaaccgtgg
    14101 tcttccgtgg ataagattcg tgcctattac ggcagatgtt tgaaggatga cgaactgccg
    14161 ctggcttttg cggctgtgga tgattccgga accctgttgg gttcggctgc agtcaagcgg
    14221 catgatatgg aaaattttcc acagtatgaa tattggttgg gcgatgtctt tgttttacct
    14281 gaatatcgcg gaaaaggcat tggcaggagg ctggtcgccc actgcatagg cgcagcgcgt
    14341 tcgctgggga taaagttctt gtatctttat acgcctgatg tgcaaatatt ttatgaatca
    14401 ttcggctggg cggttgtcgg gcgacatttc cataacgggg aatgggttac ggttatgcgt
    14461 ttggatgtgg ataaggttta agccgaatgg gcagatgccg tctgagctgt tcggacggca
    14521 ttttttatgt ttggaaattt cctgtcagat atagtggatt aacaaaaacc ggtacggcgt
    14581 tgccccgccc cggctcaaag ggaacggttc cctaaggcgc ccaagcaccg ggcgaaccgg
    14641 ttccgtacca tttgtactgc ctgcggcccg ccgccttgtc ctgatttttg ttaatccgcc
    14701 ataaagttta tccacagatt tttccatatg gtttgatgct gctccgccaa cggaaattaa
    14761 gatacggatt tgcacaaatc tttcagacgg tacagttctg acaatgcttc gcgcggggtc
    14821 aggctgtcgg gatcgaggtt ttccagggct gctgccaata taccttcaaa atgtttttcc
    14881 tctgctttat ccacaaagca gtccacattc ggttcatctc ctttttcaga cggcatggta
    14941 ctgaaaatat ccagttgggg acggtttgcg gcggcttggt tttccagtcc gttcaaatgc
    15001 ttttgggcgg ctttcaatgc gcgcacaggc aggccggcga gtttggcgac ggcgatgccg
    15061 tagctttttc cggcgggacc gggttggatt tggtgcagga acacgatgtc ccgtccctgt
    15121 tcgagcgcgg aaaggtgcat attgacggcg gcggcgtggg cttcgggcag gtaggtcagc
    15181 tcgaaatagt gggtggcaaa caggctgaag gatttgtttt tttgcagcag gtgttcggcg
    15241 attgcgtgcg cgagggcgag gccgtcgaaa gtggaagtac cacgtccgac ttcgtccatt
    15301 aaaacaatgc tttgttcggt ggcgtgatgc aggatgtagg cggtttcgct catttcgacc
    15361 atgaaggtgg agcggttgga ggcgaggtcg tccgatgcgc cgatgcgggt gaagatttga
    15421 tcgacaggcc cgattgtggc ggcatcggca ggcacgaaac agccggtgtg tgccaataaa
    15481 acaatcagcg cgacttggcg catataggtg gatttgccgc ccatattggg gccggtgagc
    15541 agcatgaggc ggtgtttgtg gtcgaggtcg gtgtggttgg ctgtgaagtg gcgtacctgc
    15601 tgttcgacaa cgggatggcg gccgttttcg atgtggacaa ccggatagtc ggcaaactcg
    15661 ggacggacaa agttccgctc ttttgccaag gctgaaaatg tggataacac gtccagcgcg
    15721 gcggcggctt tggcggcttt ttgaagctgc ggcaatgccg tccgaaggtt tttcaatacg
    15781 ccgtcaaaga gttgtttttc taaggcgagg gcttggtctt gagcagtcag cactttgtct
    15841 tcaaaggctt tcagttccgg cgtgatgaag cgttcggcgt ttttgagggt ttgccggcgt
    15901 tggtagtcgg caggtgcttg ttcggcttgg gttttggaca attcaatgta aaagccgtga
    15961 acgcggttga actcgacttt gagtgtggac aaaccggtac gttcgcgttc cttggcttcc
    16021 aaatccagca aaaattcgtc gccatggttt tgaatgcggc gcaattcgtc cagttcggga
    16081 tgaaaaccgt ggttgatgac attgccgtct ttcagccaga cggaaggttc gggcatcacg
    16141 gcggctttga gggtttcggc aacgggcagg gtttcgggga aaacggcttt aagggtttcc
    16201 aacaggctgc tgccggttgc ggacaagtcg atttgtgcca actcgaacag actgtcgcgt
    16261 aaagatgcca aatcgcgcgg gcgggcattg ccgacggcga tgcgggcggc gatacgttcg
    16321 atgtcggcaa tgctcttcag atggcattgg aggggttcgt attggctttc cagcgcggtt
    16381 acggcttctt ggcgcgctcg gatgtgggcg cggttgcgta aagggtggtg aagccacaat
    16441 gccaacaggc ggctgcccat gtgggtggcg cagccgtcga gtatggaaaa cagggtcggc
    16501 gtttttttgc cggagagggt ttgcgtgatt tcgagattgc ggcgcgtggc ggcatccatg
    16561 ccgatatatt ggctgtcggt ttcgagcgac aggccgtcca aatgttgcgg catcaggttt
    16621 tgcgtcaggc ggatatagtt caacagcgca cctgccgcgc caatcgaaac ggcgtgttct
    16681 ttgctgtcca aaccgaagcc gcgcaaatct tggcagccga aatattccgt cagcagtttt
    16741 tcccccgcgt cggcggcaaa ctgccacgcg ttcaggcgtg taacacccga tgccgtctga
    16801 agttgcggtg cgtttttact gtccggcagc aaaatttccg ccgcctgcag gcgcgccagt
    16861 tcgtcgttca atttatccgc agttgtcagc ttggttttga attcgccgct ttgcagcgat
    16921 gcccacgcca aaccgatgta ttttttgtcg ggagacacgg caacgatgcg gttggtttcc
    16981 ttgtcttcca gtaatgcgga atcggttagc gtgccgggcg ttacgatgcg cacgactttg
    17041 cgctccaccg gccctttgcc cgcgccgact tcgccgacct gttcgcaaat cgccacgctt
    17101 ttgcccaact tgaccaggcg cgccagatac tgttccgccg cgtgaaacgg aacgcctgcc
    17161 attttaatcg gcacgccgtc catctgcccg cgtgtggtca gggtgatgtc caacaatttc
    17221 gccgcttcca ccgcgtcatc caaaaacagc tcgtaaaaat cacccatacg gtaaaacact
    17281 agtttgtcgg tatgttgcgc ttttatgccg aggtattgct gcatcattgg ggaaacggcg
    17341 gatttggaca tggggctgcc ttattattag gaaaacggta ttgtagcaaa aggttttgtc
    17401 ttcgacggaa tgagactgac cgtaagcttg tcaataaagt tgtggagaca aataggaaaa
    17461 cggcaaagtt atccataaga atattattaa gtcaaaaaac atccacgctt atctgtctta
    17521 tacggcaagc cttaaactca aatttattta acaaaaaggt aatgaaaata ttggatattt
    17581 gctacttata cacaagcagg tcggcaatca tcaaaatcat cattaaaatt ttaaaatcaa
    17641 atattgaatg cgcgtttttg aaatagcgaa attcacctgc gtcaaacaaa acgcaagtaa
    17701 ttttatgtaa tagtggaatg tagttttatt tcagaataca atacaggcat cggactttat
    17761 ttcacaaccg ggagacaaaa tatgagcatc aaagtagcga ttaacggttt cggccgcatc
    17821 ggccgcctcg cattgcgtca aattgaaaaa gcccacggca tcgaagtcgc cgccgtcaac
    17881 gacctgaccc ctgccgaaat gctgctgcac cttttcaaat acgacagcac acaaggccgt
    17941 ttccaaggta ctgccgaatt aaaagacgat gccatcgtgg taaacggcag agaaatcaaa
    18001 gttttcgcca atccgaatcc cgaagaattg ccttggggcg agttgggtgt ggatgtcgtc
    18061 ctcgaatgta ccggtttctt taccaacaaa accaaagccg aagcacatat ccgtgccggc
    18121 gcgcgcaaag tcgtgatttc cgcacccggc ggcaatgatg tgaaaaccgt cgtatatggc
    18181 gtaaaccaag acatcttgga cggtagcgaa actgtcattt ccgccgcttc ctgtaccacc
    18241 aactgccttg ccccgatggc ggcagtcctg caaaaagagt tcggcgtagt cgaaggcctg
    18301 atgaccacca tccacgccta caccggcgac caaaacaccc ttgacgcgcc gcaccgcaaa
    18361 ggcgatttgc gccgcgcccg cgccgccgcg ctcaacatcg tgcccaacag caccggcgcc
    18421 gccaaagcca tcggtctggt tatccccgaa ttgaacggca aactcgacgg ctccgcccaa
    18481 cgcgtccctg ttgccaccgg ctcgctgacc gaattggttt ccgtcctcga acgccctgta
    18541 accaaagaag aaatcaacgc cgcgatgaaa gccgccgcca gcgaatctta cggctacaac
    18601 gaagatcaaa tcgtttcttc cgacgttgtc ggcatcgaat acggctcgct ctttgacgca
    18661 acccaaaccc gcgtgatgac cgtgggcggc aaacaactgg tgaaaaccgt tgcctggtac
    18721 gacaacgaaa tgtcctacac ctgccaactc gtccgtactt tggaatactt cgcaggcaaa
    18781 atctaaggct tgaacaaacc tgtggataag taaatgccgt ctgaacacat tgcgttcaga
    18841 cggcattgtt tatctgtaac ttgatattgc ggcttatggc gaacaaacaa cagccgtttg
    18901 ttcaactgct atgcggcaaa accggatttt gcccgattta aagcggggtt ggggaaagat
    18961 taataaccgt cgcgcggttt tcctcgattt gaaacctgac ttcgtaatcc gcaatattca
    19021 tcacataaat ccgttcggga atattctgat aggcggggcg cggatcttgg gcaatgcttt
    19081 ggctgataag gtttttggtg tttgcagata aattttccgc gccgatgttt tcctgccaaa
    19141 cgacttccaa ctctacgggt ttgccgctga cgaaaccgga tgctgcatcg ggtttggatt
    19201 cgataaaggg gatataaggc ttgatgtcca caatcggcgt gccgtccagc aggtctgagc
    19261 cgctgcaata gaggcggacg ggtttgccgg tttcgatgcg ttcgagttta aggagagaga
    19321 gtccgagatg gttggggcgg tgggggctgc gcgtggcgaa cacgcccatt ttttgtttgc
    19381 cgccgagccg tggcgggcgc accatttgcg cccaaccttc atccaatacg ccgtggaaga
    19441 taaaacttat ccacacataa tcgaaatctt ccaacccgcg cacgctgtct gcggtaaatt
    19501 cgggattcag ctcgatgcag gcttctgcgg cggagaccaa accgggctgg cgggcgatgc
    19561 cgaatttctg tttgtagggc gagcgggcgg tgccgatggg ggtaatggta taagtcataa
    19621 gattgtggat aagttttttg gaaaaacctt atcttatcac agccggtttt accgctttcg
    19681 ggtagtaatt tgttataata cagggcattt aaatcgcgtt gaaataaagt agcacagaat
    19741 cctcgtgtcc aagacgctct taaaaaggca ttggagtatt gataaaaaag aagcataacg
    19801 gttcgttata acgaagaggg aatcccgtat attacaccca agtcaggttg ttatctttcc
    19861 ttttggatct cacttacagg aggtgagatc tgaaattttg ggtaataaaa tcggattaca
    19921 gcttctagca aggagtacct taggtatagc taaaatggag ggggtggata tgacatatca
    19981 tatccaccta gatggattgt actccttaaa taaagaaggt aagagttttg ttattcgtaa
    20041 aatagagaat gacgatctta aatgaagaag taatgagatg attgtttcca ttgatgttga
    20101 tgcacaaaaa acttttacgc ctttgtgtcc tgacgaactg cccgtgaacg aggggcattt
    20161 gattgtcgag gagttaaatg cccaagccgc tttggcggat ttgcgtgtga tgacgaaaga
    20221 tgcgcatcat atggcggcga aatggcttgt ggataaccct gttgatatgt tgaagccgac
    20281 aggtttgtcc gatgcggatt tgacttgggt ggctcatgcg atggtcggta cgcgcggcta
    20341 tgaattgttg gacggtctgc cttccgccaa ggaatacgat tattgcgttt ggaaaggtgt
    20401 cgatcccgaa ttgcatcctt acggcgcgtg ttttcacgat attgaggaaa aactgagcac
    20461 tggattgatt gagtggctgc gttgtcaaaa tacgaatatg gtcattgtcg gcggcttggc
    20521 tacggattat tgtgttaaaa caacggtttt gcagttactt aaaggcggtc gttggcaggt
    20581 tatcgtgaat gaagcggctt gtcggggtat tgcgcccgat accattgaag cagcgtggca
    20641 ggaaatgcgt tcttcgggtg cgataatttt gaaaaacgcc gaaaaaatta aaaaatatat
    20701 taataatcaa taacttataa ttgtttcacg tgaaacaccc tgtctacata tgaaaatttt
    20761 acttgtccgt ttgtccagta tgggtgattt aattcacact ttgcccgcaa tcgaagattt
    20821 ggcgcgacaa tgctctgatg tagaactgca ttggctatgt gaagccgggt ttgcgggtat
    20881 tgcacggttg catccgtttg taaaaaaggt ccatgtaatg aagtggcggc aatggcgcaa
    20941 acatctcttt caggctgaaa cttggcggga gatgggtcgt ctgaaacagg ctttgctgca
    21001 ggaagcattt gattttgtat tggacagtca gggtctgatt aaaagcgcgt gtttcgccaa
    21061 aatggcaaaa tcccctattt atggtttgga taaacacagt gcgcgtgagg gggtggctgc
    21121 tttggcgtat gataaaaagt atgtcgtacc gaaaggtaga aatgccgttt ggcgcaaccg
    21181 tgatctgttt gcccaggtgt ttgggtatgc aatgcctgag acgcaggtat ttggtttgtc
    21241 cgttcctgaa gcaagccgtc tgaaaaattt agcgcagccg tattatgcgg ctttgcacgc
    21301 gaccagtcgg gacagtaagt tatggcctat ggaaaattgg cgggagttgc tgcagaaact
    21361 gaatcaaaaa caacaatgca atgtttatct gccttgggga aacgaggctg aaaaagtgcg
    21421 tgcggaacag attgcagacg ggctgccgtt taccatagtg tgcgctaaaa tgaatttatt
    21481 acaggcggcg tatctgttga aacacgcggt tggaatagtt ggtgtagata ccggtttgct
    21541 gcatttggca aatgccttgg aaaaacctgt ggtcggcatt tataccgata ccgatccgat
    21601 taaaacaggc gttcaagttt cggctattgc aaaaaatgtg ggcaatatcg ggcagattcc
    21661 gaccgcagat ttggtttatc aaacgctgat ggattgtgtt tcagcagata aaggctaaag
    21721 ggtcgtctaa aactgatatg gcggcatgat attttccaat ttattagata caggaatgta
    21781 tatccgccag cctgagtaaa tactttaaaa atggtataat cggtaaaatt atccgaaaga
    21841 ttgctaaata aattcgtttt taaggaaaac ggatttgatt ttataaagaa gaaggtttgg
    21901 tttcgctgga tggtgaaagc agcgttcttt ttaggagaat tcaatgaaag cactggtcgc
    21961 agtaaagcgc gtagtggact acaacgtcaa agtccgtgtg aaagccgatg gttcggatgt
    22021 ggacatcggc aatgtcaaaa tgtcgatgaa cccgtttgat gaaatcgctg tggaagaagc
    22081 cgtccgtttg aaagaagccg ggaaagtaag cgaaattgta gcggtttctt tgggtgaaaa
    22141 gaaatgcgaa gaaaccttgc gtaccgcttt ggcaatgggt gccgaccgtg ccattcatat
    22201 tgaaaccgat gtgaaattag agccgctggc agttgccaaa ctgctgaaag ctgttgcgga
    22261 caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgacg atgccaatca
    22321 agtggcgcaa atgctggcag ctttgttgaa tgcggcgcaa ggtacatttg tttcaaaagt
    22381 acaaattgaa ggtgatgaag tacagattgt gcgcgaaatt gatggcggcg aagaaacgat
    22441 tgcgttgaaa ctgcctgcag tcatcagcgc agatttgcgt ttgaacgagc cgcgctttgt
    22501 caaactcccc aatattatgg cggcaaagaa aaaacctctg gaaaaactgg attctgccga
    22561 tttggctacc gatatttcgc ctcgtctgaa aacggtgaaa tttgccgaac ctaaggcgcg
    22621 tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga aaaacgaagc
    22681 caaagtgatt tgaggaggct gaaatgagcg tattgattat tgccgaacac gacaacaaac
    22741 agttgaatcc tgccactttg catgctgttg ccgctgccgc caaactgggc aaggtcgatt
    22801 tattggttgt cggaaacggc gcatcgtccg tcgtggaatt cgcgaagcaa gtagcgggag
    22861 tggaaaaagt tttggttgca gatgctgctc attatgctga aggtttggct gaagagttgg
    22921 ctccgctggt tgtcaaattg gcggcggatt accgctatgt tgcagcaacg gcaaccacat
    22981 tcggtaaaaa ccttttgccc cgcgtagccg ccttattaga cgtaccgcaa atttctgatt
    23041 tgaccgagat tgtagataac acgacttttg tacgtccgat ttatgcaggt aatgcatttg
    23101 aaaccgtgca agccgattca gaaaaattgg tgctgacctt ccgtgtgacg gcttttgacg
    23161 cagtggcggc gcaaggtggt aatgctgagg taattaatgt taaaacaacc cctgcccaaa
    23221 acctgagtcg ttttgttaac cgtcaacttt cccattccga tcgtcccgaa ctgactcagg
    23281 caaaagtgat tgtttccggt ggtcgtgcgt tgggtagtgt ggaaaaattc agtgaagtgc
    23341 tgacaccgtt ggcggatgtt ttaggtgcgg caatcggtgc atcccgtgcg gcggttgatg
    23401 ccgagtatgc accaaacgat gctcaagtcg gacaaaccgg taaggtggtt gcgccgcagc
    23461 tctattttgc aatcggtatt tcaggtgcga ttcaacacgt tgccggtatg caggacagta
    23521 aggtgattgt tgcaatcaac aaagatgccg atgcgccgat tttcaatgta gccgattacg
    23581 gattggttgg cgatttattt gaaattgttc cacaattaat agaattattg aaaaattgat
    23641 gtttcacgtg aaactttcag gcgactttgt tattaatgag gtcgtctaaa aacatttcag
    23701 catgattacg acttataaaa ccattacttc cccgacgcag gctgagttta aagataaagg
    23761 cagccgtttt attgcatttg cctatccgat tcggacattg gctgatgtga aaaaatacct
    23821 caatctgtta aaggaagagc attataaagc gcggcattgg tgctatgcct atcgtttagg
    23881 tgtgaacggg atgcagtttc gcggcaacga tgatggagag ccaccaggaa gtgctgggcg
    23941 gccaattttg ggacagattg attcggtggg tatcactgat gttttggtcg tggtcgtccg
    24001 ctatttcggt ggtgctttat tgggcgttcc cggcttgatt cacgcataca aagaggcaac
    24061 ggctcaggcg ttggcgatcg cggaagtggt tgaaaagaat attgaaaaaa ccgtttggct
    24121 gaaatgtgag tatccgtttt tgaatgaagc aatatgcatt gccaaacaat atcaggcgga
    24181 tatattggag caggatttgc agttggattg ccgattgacg gtaagtttgt cgttggcaaa
    24241 ttatgaggcc tgtgttgcgg cttggaagaa tacacggcag gttgaagtaa atacggaaaa
    24301 gccttttgaa tgaaaggttg tatgaaatgc tttgtaatgg tatttcaggc aacctcttcg
    24361 ctgattaatt ttcttgtgta tatctgtcta aggtatcgac gctggagcat aaatatagcc
    24421 aagccaattg atggaattcg cccggcgcgt tccataatgc atcttcactc ataatgctat
    24481 attcgccttc tgtttgcaaa ttcagttcta ccccgtcttc aatagtctga tggcgcacct
    24541 gatactcgtg ggtatagaaa ttgtccatat cgcgaattaa agcgacggca tatttccaac
    24601 ggcggatgtt ttcttccagt tgcagtagta aatgacacca ttcgcggtat ttgctttgga
    24661 tttcatcaat acgtttttgc gtgtttgctt gttatttttt aatttgtgaa taagttgaat
    24721 aggttgcaga gtggaatgaa gtggtctttt gtttgattct actaaaattt cttcagatgg
    24781 gacaaaagtt cctttgtatc gagttttcgt taaaagtaca tatttgtcaa ggagatggca
    24841 tgaaactgct ggttatcggt aatggcggtc gcgaacacgc gctggcttgg aagttggcgc
    24901 agtcgcccaa agtggaaaca gtatttgttg cgcccggtaa tgccggtacg gcgattgaat
    24961 ccaagctcca aaacattgcc ttgaccgcgt atcaggattt gattgaattc tgccgtaaag
    25021 aaaatattgt ttttaccgtc gtcggccctg aagcgccttt ggcggcaggt attgtggatg
    25081 attttcgtgc cgcagggctg aaaatatttg gtccgacaca atatgcggcg cagttggaaa
    25141 gttctaaaga tttcgccaaa gcatttatgg tgaaatacaa tattccgacc gcgcaatacc
    25201 aaacctttga aaatgccgat gccgcacatg attacgtcaa tcagaaaggt gcgcctatcg
    25261 ttatcaaagc cgatggtttg gtggcaggta aaggcgtgat tgtggcaatg actttggatg
    25321 aagcgcatgc tgcgattgac gatatgttgt tgggcaataa aatgggcaat gccggcgaac
    25381 gcgttgtgat tgaagatttt ctgcaaggcg aagaagcgag ctttatcgtt atggttgatg
    25441 gcaatcatgt attacctatg gcgaccagtc gagaccacaa acgtctttta gacggagata
    25501 aaggcccgaa tacgggcggt atgggtgcgt acagtcccgc gcctgtggta acgcctgctg
    25561 tgtacgagcg tgcgatgaac gagattattt tgccgaccgt agcgggtatg aaagcggagg
    25621 ggcacgagtt caccggcttt ttgtacgcag gtttgatgat cgatcaaagc ggtgcaccct
    25681 atacgattga gtttaactgc cgtttcggcg atcccgaaac ccagccgatt atgagccgtc
    25741 taaacagcga tttggcggat ttggttgaag cggcaataga cggcagactg gacagcgtca
    25801 aagcggaatg gaatccgcaa actgccgtgg gtgtggtact ggcggcgcaa aattaccccg
    25861 aaacacccaa gaaaggcgat gttatttccg gtttggatga cgtcaaccga atcggcaaag
    25921 tattccatgc cggcacaacg gtaaacgaga aaggcgacgt gttgaccaac ggcgggcgca
    25981 tattgtgtgt tgtaggattg ggcgacgatg tggcgcaagc gaaagccaag gcgtatggcg
    26041 cgttggaaaa aatcagcttc gacgggatgc aataccgtaa agacattgcc gataaagcga
    26101 ttaaccgtta attagaaaga ggatgatgcc gtctgaagcc aagtatgact gatgcgcttc
    26161 agacggcatt ttatattatg ttattttcga gaattatcgc agcatcagcg caacgtaagt
    26221 taagcgttta tttgaaaaaa ggcggtttgg ttgcttatcc gaccgaatct tgttatggct
    26281 tggggtgtct tccgacatta gccaaagcac tcggtaagtt ggcgcatttg aaaaaaaggc
    26341 cgcaacacaa gggtatgatt gtcatcggga atcaacttga acaattgcag cctttacttc
    26401 aaatgccgtt tgaaaacatt caaaccatgc ttagaaacga atggcctgct cctaagactt
    26461 tcctattgtt ggctaaatct ggcgtattac ctgcgttgcg cgggaagagg cgcagcaagc
    26521 ttgcggtcag agtgcccgat catacaggtg caaggcgttt gtgtcaggca ttgggtatgc
    26581 ctttggtttc cacttcatgt aatcgggcgg gtaaacgcgc gtgtcggacg gagagggaag
    26641 tcagaaggca gttcggacgc gatgtgtgga ttgtcggtgg gagaatcggc aggcagaaat
    26701 cgccgagtca gattatcgat ggggaaacag gtaaacgttt gcgttagata tggatgcttg
    26761 agtgaaaact tttttgcata cacaaagaaa aaattttttg gatggtattt ctctacatga
    26821 ttgttatcgc acggcgataa aggtagaagg acgcaacgtc tgctttgatt ttgaggacgg
    26881 ttttgtcgtt ctggacaata atccgaacaa tcaagcgggc aagcatctga aaacagattt
    26941 ttcccgtgtt gttttaaccc atgaaaatag aaacgccgaa gacgactatt tggtcgatat
    27001 ttttgaagac atcgtctttt tagggaaacg tttgtttaca gtcagaaaat ttcttgattt
    27061 ttctgaattg cttgaaatga tcaatgcaca aggctatttt cttgaatttc tgtatctgta
    27121 tgaatgtatc ggtgtcaaaa caagc
//
LOCUS       DAXFHF010000025        25790 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXFHF010000025 DAXFHF010000000
VERSION     DAXFHF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25790
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.025"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(51..707)
                     /locus_tag="ACFIRM_000973"
     CDS             complement(51..707)
                     /locus_tag="ACFIRM_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC3028625.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(716..1261)
                     /locus_tag="ACFIRM_000974"
     CDS             complement(716..1261)
                     /locus_tag="ACFIRM_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC3028626.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(1261..1809)
                     /locus_tag="ACFIRM_000975"
     CDS             complement(1261..1809)
                     /locus_tag="ACFIRM_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028627.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(1837..4413)
                     /gene="traC"
                     /locus_tag="ACFIRM_000976"
     CDS             complement(1837..4413)
                     /gene="traC"
                     /locus_tag="ACFIRM_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC3028628.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(4423..5004)
                     /locus_tag="ACFIRM_000977"
     CDS             complement(4423..5004)
                     /locus_tag="ACFIRM_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC3028629.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(5001..5720)
                     /locus_tag="ACFIRM_000978"
     CDS             complement(5001..5720)
                     /locus_tag="ACFIRM_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC3028630.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(5730..7043)
                     /locus_tag="ACFIRM_000979"
     CDS             complement(5730..7043)
                     /locus_tag="ACFIRM_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC3028631.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRSIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(7045..7779)
                     /locus_tag="ACFIRM_000980"
     CDS             complement(7045..7779)
                     /locus_tag="ACFIRM_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC3028632.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKDTLFIQNGSYTAADMQGFSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(7781..8446)
                     /locus_tag="ACFIRM_000981"
     CDS             complement(7781..8446)
                     /locus_tag="ACFIRM_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFC3028633.1"
                     /translation="MKTEDMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     RGN"
     gene            complement(8443..8724)
                     /gene="traL"
                     /locus_tag="ACFIRM_000982"
     CDS             complement(8443..8724)
                     /gene="traL"
                     /locus_tag="ACFIRM_000982"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC3028634.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(8746..9042)
                     /gene="traA"
                     /locus_tag="ACFIRM_000983"
     CDS             complement(8746..9042)
                     /gene="traA"
                     /locus_tag="ACFIRM_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC3028635.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(9098..9619)
                     /locus_tag="ACFIRM_000984"
     CDS             complement(9098..9619)
                     /locus_tag="ACFIRM_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC3028636.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(9589..10050)
                     /locus_tag="ACFIRM_000985"
     CDS             complement(9589..10050)
                     /locus_tag="ACFIRM_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3028637.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            10561..10980
                     /locus_tag="ACFIRM_000986"
     CDS             10561..10980
                     /locus_tag="ACFIRM_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028638.1"
                     /translation="MNETDLPKLLSVINRFTDIDKNWSCVLLFWIQRASGYLEMMTLD
                     DDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEILFLMAQYGSEYWNSLEESF
                     EEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            10977..13529
                     /gene="mobH"
                     /locus_tag="ACFIRM_000987"
     CDS             10977..13529
                     /gene="mobH"
                     /locus_tag="ACFIRM_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC3028639.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            13526..15568
                     /gene="traD"
                     /locus_tag="ACFIRM_000988"
     CDS             13526..15568
                     /gene="traD"
                     /locus_tag="ACFIRM_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC3028640.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSDKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            15558..16253
                     /locus_tag="ACFIRM_000989"
     CDS             15558..16253
                     /locus_tag="ACFIRM_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFC3028641.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWPESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPPYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(16683..17195)
                     /locus_tag="ACFIRM_000990"
     CDS             complement(16683..17195)
                     /locus_tag="ACFIRM_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028642.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(17286..17681)
                     /locus_tag="ACFIRM_000991"
     CDS             complement(17286..17681)
                     /locus_tag="ACFIRM_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3028643.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(17668..19590)
                     /locus_tag="ACFIRM_000992"
     CDS             complement(17668..19590)
                     /locus_tag="ACFIRM_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3028644.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(19667..20038)
                     /locus_tag="ACFIRM_000993"
     CDS             complement(19667..20038)
                     /locus_tag="ACFIRM_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC3028645.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            20234..21541
                     /locus_tag="ACFIRM_000994"
     CDS             20234..21541
                     /locus_tag="ACFIRM_000994"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC3028646.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            21534..21968
                     /locus_tag="ACFIRM_000995"
     CDS             21534..21968
                     /locus_tag="ACFIRM_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028647.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(22144..22413)
                     /locus_tag="ACFIRM_000996"
     CDS             complement(22144..22413)
                     /locus_tag="ACFIRM_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC3028648.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(22595..25057)
                     /gene="lon"
                     /locus_tag="ACFIRM_000997"
     CDS             complement(22595..25057)
                     /gene="lon"
                     /locus_tag="ACFIRM_000997"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC3028649.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVATDLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            25309..>25790
                     /locus_tag="ACFIRM_000998"
     CDS             25309..>25790
                     /locus_tag="ACFIRM_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC3028650.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHKKDTTLIDNS
                     "
BASE COUNT         6601 a         6092 c         5930 g         7167 t
ORIGIN      
        1 tgggagtagt aataacggaa tcagtaattt cagcggtgtg acaaatattt tcacggtttc
       61 atgacctcta cacggagccg gcccccttct tgggagacca aacttggaac ttcattcagc
      121 tgaaaacttt tggacatccg cccttcttgg tcgtaataaa ccgcctgatc catttctact
      181 gataactcat accaattgcc tgcagtcaga ataggcatga atgattcgcc atattctgct
      241 gcaagtttct tggccagttc gatttggtca ggttcccggc cgtcaaagaa aaacattttg
      301 aatgggattg gggctgcttc caaaggtttg acaacagtac cggcaggagc aataatttga
      361 ccgtcttggt cataaattgt ttcggttaag gtataacttg ggtcgaagta tcgaacagag
      421 cctttagttg ccttcgccag tccttccaag ggctgtagat ttaaggcgga gcgatttatt
      481 ctatcttgga cctctttctg cattgccgcc aattggccgc tgtcttgttt agctttcaaa
      541 cgcgcttcaa tttcgcggat tgcatgtttt tccgctattg ggtatgtggt gccgattttg
      601 tcgccaacct tctcgacaga gggtgtcgaa gcggctaccc atgaggaaat tccggttgca
      661 agtattacta gcgatgcgac tgaagctatt tttttcttgt gggacatact cactcctaaa
      721 aaatggggac cgccttacca ataatttgcc ctgaatggat taagccggat aattcatatc
      781 gactgtccaa gctgtctttg tgccctgttt taacccacat gaaccgttgt ggaatttccc
      841 cttcgaatgg gttgatggcc agcttcatgc catccaggga gtattctttt gcagtaccta
      901 cctctttccc atctatgaaa aaagtacgat tcgccttcgt tacatgattt ccgggaagtc
      961 cggcaacctc tttgacgacc tgcgtaccaa tagggtaaaa accaccttgc catgcaaagg
     1021 ccacataatc gccctgcttc agatcactta gcttgttcgc atcttttttg atgaagtaaa
     1081 gatggtgagg caaggaacgg gaaagattaa atgccaaagc ataatgttga ttgaatgccg
     1141 acaagcagac cgcaactgct aaaaaagggg ttgcatattt ctgacaaaac tccctataaa
     1201 gacgtttcat cactgctttg ttgctttctt tttgtgatat ttttatcatt gcctcaaaca
     1261 ttaacgatcc atttcttctt cagtaaccgg ggtgccttta ccctcttcgg ccatcggttt
     1321 cggaattgtc ttttcaactt gttgatgtac cggtgccgcc actacagctg tggccgggac
     1381 acgcttatcc aactctttca taatgtcagc agtaacatca accacctctg tgttccgtcc
     1441 gaccaatgca gatttgacaa agatgttaca gccgcaccga attacctttt cttcgataac
     1501 ctgttgcaag gtattcatct ttgcttggta gttttggagg atcgcggctc gttgccggtc
     1561 ggaaattcca ttctgcagtc cttgattctt cgcctgttcc cggtaagtca tggagatacc
     1621 ggccaaatct acaatatgga ccggtttctc atgggttttc atgaaattcc atacgcctaa
     1681 tgccaatgcg gcaatagaaa ttgcgccgat cacaaggctg cccaatgaaa cctctacgcc
     1741 gttatcctcc ggattctttt ccggatcggt atttgccgtg ttttctttct tttcttttgc
     1801 ttcagtcatc ttattaatat cctatcctct gcctaattag cgggttgcgg cacggtccct
     1861 taaaactgct tcaatggcct cggctgtatt caacccctga tttctgtaat ggttcacggc
     1921 ttcaacgtct tccgcttttg agcttgaggc aagaacgttg aatgggtccg agtacaacct
     1981 acctactcca taacctatcg gggtagagac aaatatttca ctgtagtagt cagttttgct
     2041 caaagttcgc agaatctgca taatcccgtc attgacttca aaaggttctt tgtcttgaat
     2101 ggttttgagc gatttagccc cggtacgcaa ttggaaaaca aagtcggcat tctctaatgc
     2161 cgcctcagcc gccttagtct tgtagtagtc atttgcacct tgcgtcagag tgccgcatgc
     2221 acctttgtac ttacggacac ggcggtagcc ttcctcaata aaacgagcgg ctgtacccgt
     2281 gtcgcccaac aaagcccaag cctcgtcaat catcataatt ttgtactggc ttcgtgatag
     2341 gtacatgccc tgcataatgc ggtacataat cagctgcatg atgatctgtt gcagatccgg
     2401 tttggttttt aactcttcaa gttccagtac gaccaggtca tttttaaact cgacattcaa
     2461 ttttccattg aagtaactgc cgtagatacc ttccgaacag tatgtgtaca attggtcggc
     2521 aacggccacc atagacaaat ctcgttcacc gggctttcct ccttcattac tgccggtaat
     2581 caaacacttg agacagcgtt cccttacatg atcgaccgtg ccttcttgtc cgaattcgta
     2641 ccatacttct tcgattatcc gttgaagctt ggcttcctgc tgttccgtta gggagtctga
     2701 cggagaggcc atcaatgaga agattagttt caacatctca atatcttcgt tgatgtccgt
     2761 taccatttgg aatggcggga agcacaggcc tgcttcatag gtaaattcaa tatactgccc
     2821 gccaatcatt tggcagaatt tttcatacga acggccaacg tcgataatcc atacctgagt
     2881 ccccagcccc atgtagttgc gcacaatttc attggcaaaa aatgatttgc cggaaccgga
     2941 agcgccaata atggcaaagt tatagttacc tgccgggttg gcaaataagt cgattcccat
     3001 ggcctgcccg cgttttccga acaaaccgat gacaggtctt cctaaaccgt gccactcgcc
     3061 gataacggga gacatacaga cagcattagt catggttttg gttgaccatc ttttcttact
     3121 gtccaaatat ttctcaacct ctttgtcgat agccattggg agggcagtca aatatgaccc
     3181 cagttgaaga tattggtcac gataaagacc gaagccctga gcctgccaaa tcgataccgc
     3241 ctggctttca gccaagttaa tgttttcggg acgctcccaa atgacaactt ggtgcaacaa
     3301 actcaccaga ccgccgccgc ctttgccgaa cgcttctagg catatgtcgt aatcacgttt
     3361 gatttttgct gcctccggca taactgcggc catcttggat tccgccgttt gtttggcgcg
     3421 ggccgctttc agttttacgg tattgctgga agtgtcataa tcacgcttga acatcgccat
     3481 ggaaatcaga aatggtgatg tgtaattcat gtttgcctca atgacatcac caatcaatgc
     3541 gcccatattg gacagatgaa actcttcagg gtagcgattg gcagaatagc attgagcaat
     3601 cacgcaatcc cccgcggcac ggttgccaaa acgcagcccc gagccgctat caagaacctt
     3661 gatagatgtg cgggtctcaa tcatttgttc actcaagaac ttggtttcat cccaaacagg
     3721 atcgatttca tcttgttgtc cggtaaacat cctattaggg ttgagtagga gcgttgtcca
     3781 agccaataaa tcgtctgcat tccaagttcg gccgtaaatc tgcatggagc ggagtgaatc
     3841 gcgcatgcgg gcaaggagcg aaaccgcctt gctgacttcc actgcattat caggtggaat
     3901 attcaaattt acgctgagga tacagcgcag atttcgtaaa cgaatggccg tattttctac
     3961 caagaccttc tgtgtaccct tattccaaaa ttcagtacgt tttttggcca aagtttcgaa
     4021 agtgcttcgc atcggatcgt ctataggtcg tgatgcttgt acttgctcat attccttgaa
     4081 gtaatttctt aaatcggggg agccgaaaat ttgaacctgt atcggcgtct tttcaggaag
     4141 cagcgcatac aggctgcccc acttaccgat caggtcgtca ctgccgccta aaaatgggtt
     4201 aagttccaga ataaagccca tgcccaacgg tttgtcgcct ttaacggaca tcagcgtaaa
     4261 caactctgtt ttggtatcgt atgcaccgta attgagatag tcgctcaaat ggtcaaagtc
     4321 aggatcgaca gggttgttgt gcaaggcttc catcggttga tcgacatcat ccgaaccggc
     4381 caaccaattt ttaaattttg acaggacacc cattacatac ctctatcgaa cggtaccggg
     4441 aatacccgat gtttcctgca gatgaagatt cacgccaaca tcggggatgc ggtcatttgt
     4501 gttgtttgaa ttggttggag aagtctcaac cgaactgcct gaagttttgg ccccatcccc
     4561 caataagcgg atgggacgat attcatttat aatctgttgc ccgttgtggg caattaacca
     4621 atcaccctga ttcagaacga catatgtgaa actttggtca tttaaatcgc cgtccgtatc
     4681 aagataggga gcgacccaaa tgcgagcaat ttcactttgc gagcgaacgg gcgtaccgga
     4741 ggaataagct tgcgttaaca ttaatgtgcg aacactttga tcagtgtaag gttgtccaac
     4801 ctgtcttttc ataccgtctt ttaaaacacc tgcaacagat gctttatagt tactgcttat
     4861 agattcacaa taaggatcgt cactgttccg ggcattaggg catcggaatt tttcactacc
     4921 gccgataccg gacatggtta aggttgaaca tccgctaaga atcaatattg ccgcagtaat
     4981 cgataaggct ttcaaacgca tcattctgct cccgctccat ttaaccattt ttccaattcg
     5041 gcacgcggca tggcaccgga tgtacgccga ccatctttat gaatcatgct tggagtaccg
     5101 cgaactttaa gctgctctgc gagtgcaata ttttctgata cagggttttt acagttaccg
     5161 gccgccggtt ctttatccat cagcatgtag ttattccatg ctttttcacg atctttactg
     5221 caccagatat gttctgcttt tgaaattgca tcagggtgga gggatttaat cgggaacatg
     5281 aaaacgtatg cggtatagtc tgtcatgccg gccaatgttt cttccaaacg acgacagaac
     5341 gggcaatccg ggtcggaaaa aagtgcaaat tctcgcttgc ctttgccgcc attaccttta
     5401 acgattttga tggcgttttt gaatggcagt cgggaataac tcttttgtgt taccgctgaa
     5461 cgggccatct cggtcaagtc tttctgctct tgcatatcgt acaacgcgcc aaaaatgaaa
     5521 tagcgaccgt tttcttgtac ataggcaatg ttgtcaccca ttgtcacttc ataaattgaa
     5581 gccatggggg tagcctttac tgaagaaaac gtggtattcg gatacaactt gtgcagagtg
     5641 cttccaacag ttttgacatc ggcaaaggcc gtctgaaaag ctgtgattgc tgcaatagtg
     5701 gccaagattt tctttttcat agatggatcc tatttggttt cgccggtatc aatttcgata
     5761 ccttgagtca gtacgacatc gacttgacgg cctgcattga cttcaactac cgggaagacc
     5821 ttatcggcca gttttatgta atactcagca agcctgtcag ctgcaccacc gataccagat
     5881 gcaatccctg cacgatattg atcactgccc cgaatagagg ttgtgctgcc aaatgcggta
     5941 ttattcgtca ccttgaatgc ttccgatacg cccttaccca taccgctaat cacgccggac
     6001 atcagtgcat ttgccaagag ttggccttgt ttcgttacaa gattacctct cacgcctgtt
     6061 ttgccgtctt caccggcaac gtaacctttt accggcatgt cgatggctcg gccatcggta
     6121 cctacacagc tgagattctg cagtctgatg taagcgcgtt ctgaagatat atcgccgtaa
     6181 gcctgcccgg ttacgaaaca ttctctgaaa ttgtatttaa accggttggg aagttgcgca
     6241 aaatccgtta cgcgcattaa aacagggtgg gggctggcat tttgtgcctc acccccggtc
     6301 ggtgcgtcaa ccccacctaa cagtacggat cggaaaaaag taccgctagg tatatacgag
     6361 tcacgcgccc gaaacttgcc ttgcgagacg acaaccttac cgccgtcttc aactacttct
     6421 atattcatac gcggggtttt tgatttaacc cactccgctt cttttaattg cggggaaacc
     6481 cgtacatttt cattcggatt tccggtattt accgatccgg tcgttccagg ttgcagctct
     6541 gcttcactca tacttcctga aacgattttc cgttcaatgg acctatctgc cggaggcgcc
     6601 ccgttcggca atgtgcctgc cggaggggcc ggaggaagcc ggccgttatc cccgataccg
     6661 ctaattgcag gcggtttgcc taatccatca ggccccagcc ccttacttgt atcaggatta
     6721 ctgctgttct ctttttcttt taactcctga accatggttt ccaattcgct gatttttcga
     6781 tttgcatatt caatcttcga actttctgtg ttcatccata aggaattgtc cgtaatgcct
     6841 gcttgggggc ttgtaaaatc agttctgaga atctgccgtt tgttttgaac ctgtgtgact
     6901 tcggcagatt tctttttatt gccttcaatg acataaattg cactgcccac aatcgccgcc
     6961 atgctgcccg caaccatgcc cgcgatggct aactggcgtt ttcgcgctgc tgaaagttta
     7021 aatttgttta ctttcaccct catctcaacc tcccggacgg ctgataataa atacgtcggt
     7081 aatttccccg ggttgtagga tctgtttccg aaccgctacc gccaaaactc cttgtcggta
     7141 aaattcctgc tctctaattt caagttgttt agtacctaaa ttagttaatg taaaagaaaa
     7201 gccttgcata tcagcggcgg tgtaacttcc gttttgaata aagagggtgt ctttccataa
     7261 tgggacggtt tgatattcat gactatgatt gatgcccata tcgccggtag tgccacgcat
     7321 catagaggcc atcatttttt tgatggcccg tacatattct gtactattca tcgttacagg
     7381 cattggaccg gtttgcgaag ccaatactgc tgcttcacgg cgattagctc cagccacatc
     7441 gattacgatg ttgtcaccct gttttgatgt aggtttaaga accagcaaat aggtcttacc
     7501 ttcttcgctt ataacgaaaa gatttgtcgg attggaggtc aaactacgga caaacagttg
     7561 gcctgttgtt gtatctttct gaagctcaag atcaccttcc ataaatttcc atgatgaaat
     7621 tcgaccgcct tcaatggcga ttctgctcaa gttccgttta cttattgaaa caacgtgagg
     7681 tgtattttct gttgccggca ctcgttgcgc tgcaaaacta gtcgcgggtc cggctgccaa
     7741 caaggcagaa aagacaattt tttttaacag tgaacacata tcaattacct cgattaccgc
     7801 ctttaaattc tgttgccgtt ttactgattt ctacatcctc ggtttcaact tcggattttt
     7861 cctgacctgc gggttctgct ttagttacac gcttttgaac ttctttaatc gataataatt
     7921 gcagtgttcc ataattattt ttgaaagaaa taagtagctc gcggtctttt tgctctacca
     7981 gggcattgtt gcgtttaacc tcaaaaaccc cacgaatgga aactgtattg tctgaaatgt
     8041 ccgccgttgc ttctgtcgcg ctaaaagtgg tgccgacatt atttttctta acccataagg
     8101 catttaaaga cagctccctc tctagggcat ctttataatc aggtgaaaca atcttcaata
     8161 attgactatt ctgataatcg actgttttag gggttacatt taagagaaga taggcagcat
     8221 agggagccat ttcttcaaga tattctttag aaaaagcgat gtttgataca gtcattgacc
     8281 gccggatttc cggcggggtc agcgtcgttt gaacaatcgt acctttgaag agcagggtcg
     8341 cggacaaaaa tagattgaca gctatagaca ttcccaaaag tatccaaaaa aagaccttta
     8401 tgccggttct tatcgccaat tcactcaaca tatcttcagt cttcaattta gaaactcccg
     8461 tttattcgtt tcaggtatcc gtttgttttt ttcattaatg ggtaagtacc agtaaagaac
     8521 atgcaaaaaa aaccattgat gcttgccaga cttaagcttg ccccacctcc atgcaatgaa
     8581 aaggctaatt gacatggaaa tgaaaaagtg acgagagaca atacctatac cgaagccgga
     8641 cataaataat ataaaaacgt ccaagtccca aataaatgcc tttttgggat cgtctatcga
     8701 taccggcaca tgataatctc tcatcacttc tcttatctag tttgattaaa taatcaaggt
     8761 aaacatacct ttaatgatgc ccgggcctaa tgttgcggcc aagccaacac ccaaaccgac
     8821 gatacatggc atgatggttt gtttcatcag gcctaccgcc aaaccgatca agaatgcagt
     8881 caagcccaaa gccataccca gataaccttc agaccagcct gttaccatgt cagccaagcc
     8941 tttgaattcg gcacctgttg tagcggccat tgccaagtta gcatacaatg ccaatgcaac
     9001 cgcactagcg gactttacta atttattgcg tttgatattc attatgaacc tcattaaaca
     9061 gagcttatat cgccaagctt ttgcgtccga tcattcttta ccaagtgatt acaacggttc
     9121 tcgtatcatc actattgcag caggccgggg aacttgctgt tcttattttt gcggatacat
     9181 tatgtttcct cagccattta cctattactg ctgcccgttg tttagccaaa cgtctattaa
     9241 ttgcagtatt tcgacctccg atactgtcgg cccatccgcg taaataaacc gtctgagtat
     9301 ttttcaattt aggcagaaca cgtttgagcg ttctcacgcc ctccgcaccg aggtgcgaac
     9361 tgcctaaatt gaaaaacacc ctgatctcat ctttttgaag attggtttgt gtttgagcat
     9421 aaaccggttg ttccgccaaa accttgcgct caaatcccga acactcttca ttgcggcaga
     9481 agacaaattc gcccagctga ttttgttcca ctgtataagt tgcgtcttga ggcagcccgg
     9541 ccacttcctg ttttggggcg gcacaggctg tcagaatcat tgcggaggct acgaagagcg
     9601 tgtttttaat cgtttccaat tttgatatac cttatttgcg taagtcattc ttgcgcgata
     9661 gcacgcccgg cctttaagtt tggtacatgc ggcgttataa gcaccaactg cttcccatga
     9721 tttcccatgt tgccgataac tatttgccaa tatccatgca ccaatatgta catttgtgca
     9781 tggattccat acatccgccc ctgttaaacc aaatttcttg tggagtacgg gtagccatga
     9841 acggttgata cccatcaagc caatatcagc tgtatgactt tcaatattcg gcctgaaagc
     9901 gctctcggtt aatgcgatcg attgcaacat ggcgggatcg atgccatatt tgctgccggc
     9961 ttccttataa caaaaagcaa ctgctcttgc agatgtgagt accaaaacac ccggtacgac
    10021 gaactgtaaa agtatggccc ttaatctcat gtctttactg tcgttgtatg tatgactaat
    10081 ttttatctcg attcgacaat ttttgttaaa tcaaacgaaa ttaactaaat tttttagaaa
    10141 tgtttcttta aagatagatt atggtataat taaaagttac gcttatattt tatgaaatta
    10201 ccgattcata acatattgat ataaaaagga atacgataag ggtaaaggct cttatccaaa
    10261 agccaaaagg ccgtctgcta aaaaaaatcg cagccggcct tttttaaaag ctgtaacggt
    10321 aaagcaacag ccttaatggt taaaggaagg gttcaaggga aaaagggtaa aagttaaagg
    10381 taaaacaaag gtctgcatgt aaaaatgcag acctttgcct attaaaaggg gcagcattat
    10441 accttataaa tcttattctg tgacaattct aattaaaata acatttttta ttttgatttt
    10501 tgacatggca actttttttt attatatgaa tggtttgaca accctgtctt tttaatcaaa
    10561 atgaatgaaa cagatcttcc caagcttctt agtgtcataa atcggtttac ggatatcgat
    10621 aaaaactggt cttgcgtgtt gttgttctgg atacagcgtg ctagcggtta tctggaaatg
    10681 atgacgctgg atgacgatga aaatacggca accttcctta tcgaaaaact agagaaacta
    10741 ctagagccac taccgataga tattgataac acgacttttg cagaagcact tgccgatgac
    10801 tttgaaatct tatttctaat ggcccaatac ggttctgaat actggaattc acttgaagaa
    10861 agctttgagg aattttgtat tagacatact acgctaccta atcaattgat tgctgatatt
    10921 ccacatgcaa aaaaggaaaa agtcaccatg tggctaaaat ctttgcttaa atcatcatga
    10981 aaacaagcct tctcactatt gcatcctcac taatgcttgt cagcggctca atgctatacc
    11041 tgataaatac gagaaaaagt acagattccg tgtcacataa tgatgaggtt gcccatatca
    11101 atcagaacat agtaaatgac cggtttcgca ttttaaacgc gcatgaactg attcaggtgc
    11161 tcgacttaag ccctcaaata tccggtatta agatgaactt agggttatcg gatgaaaact
    11221 ggagtaagga tgcccttccc tttttggaaa aatatatagc ctttgttcaa aggcttcccg
    11281 ccagtgagtc tcatcaccat gcaggtgatg gtggtttagt ccgccataca ctcgatgtgg
    11341 ctgcactggc tcttgtggca tctacatcgc aatcatggcc cccaaatgcc aaaaccgagg
    11401 aaatagcgaa gaaaactgcc gtttggcgtt atggaatcat gtgtgccgcc ctgcttcacg
    11461 acgttggtaa aactgtgacg ggatttcagg ttgaactgtt tgattcggct atcagtctcg
    11521 aaaaattatt atggttgcct gatacaggct ccatggctga aagtggcaag ctttattatc
    11581 gtgtcgaatt tcctgatgca aagtctgctt acagtacaca tgccgaaatc gcctggacat
    11641 tctttcaagc tttggttcct tcacatgtca gacaatggct tgccacaacg gatccgaatt
    11701 taatgattac tttgagaaat tatctgagtg ggaaaaagga tggcagtcct cttgagcagt
    11761 taataaaaaa tgcggatatg acctcagttt caagggattt acgaagcggc agccgtcaac
    11821 gcttctcaac cgccaagcgt aaaccattca ttgaaaccat tatggaaaca ttgaaggaga
    11881 tgttatccga tagaggagtc catttcagca tcgcaacaac tgccggtggt gacctgttcc
    11941 gtaagggtga tcgcatctat ttgatgtcaa aaaacgtacc tgactacatc cggcagtatt
    12001 tgaggaaaaa tcaacatcct gctgcaggct catttccggc tgacaaccaa aggatatttg
    12061 acacattatt cgagtaccgt gccgtcattc cgccggaaaa cgatcctcac agggcaatta
    12121 accatataga ggttgagttt acccgtatgg acggtgaaaa ggtccgtaat atcttcagtg
    12181 tactttgttt caatgccaaa accctgtatc cggacggaga ttatccgact gagttcttag
    12241 gtaatctttc agaagtttcc gccaagaagg aaataccgga tgttgtagta tcagaggata
    12301 aagtgtcaac tcagatacaa ggtgccgaag ataacccagt cactatgacc aaggacaaaa
    12361 tggacttcct gtctatccaa ccggtgacag cagaacaacc tcccctacct gaaaaaaatc
    12421 aaactgagac agctaactcg gcttttgaca acagtatgaa tactgctgct ccagcggaaa
    12481 ataaatctag cgagtcagag aaagctaaag aaccgggtta cggcacaatt gataatttaa
    12541 ttgaaaactt taacctgata gaagctgatt cagaacaaac agaatcaacc aaatcagaag
    12601 ccgagacagc agaaaatgcc gttaacctgg ttcaagaaaa tataagagta gaagttaaaa
    12661 ctgagacagt ggacaaacct cgcgctgagg ttaaagaaac tgagacagat aaagcaccaa
    12721 aaacaattaa atcaggaacc aaagcaagcc gtaagaaact tgccggcctt ttcgcagaca
    12781 atcgtaaacc tattggaaaa acagccgaac ccgatgcaca gattaaaaca actgagacag
    12841 taactcatca aatagattct gaagcagttt cattgcctga atctgaatca gcacaaaacg
    12901 gtatgcgtga actggagcag ctaagactaa gagaacctac atctagtcca cgcccggttc
    12961 aagtcatgga tgactccgaa ggtttagata gccttgcaag cggggtaaaa aatatggccg
    13021 aactcgaagc catagtttct gaacgtaatt tgaaaaagca atcagaaaca gagtcgaccg
    13081 aaatagtcga ggattcagtc aaagccaaac atatggaggc cagagaccgt ggaatgcagt
    13141 ttttacgatg gctggcagat ggattaggag acggcagcat agcagtcaat agaagtggtg
    13201 caacagtcca ctttattgaa caggggatgc ttttggttac gccagccgta tttcgagatt
    13261 acgccggcgg cgtgttcaat aaatctgata cggaaagtct aggccctctt acacaaaaag
    13321 gctttgaggc tttgaggttg catgcaagaa cgaaaaaaag ctcattgcat agggttctga
    13381 ctacaaacgg tagcaatcgc agactcttct attgctacct tatccctgaa cataatatca
    13441 aacatatcat acagccaggt tcaagacctc agaataatac cgatattaaa ttagatgaat
    13501 ccgacttatt agtaatggaa caaaaatgag tgcccacttc cctgaaaacg tttatcgatc
    13561 accctatgaa tggatcagtg tagccgcata ctccaccaca gcggtttcag tagcacattt
    13621 gcccgtacct ccgacaattg cttatggcag tttagctatt ttttcgttgg tttcagtcaa
    13681 acgtgcgtta tcagcattac gcattatgcg tttccgtaaa aatattcgga agttgcccta
    13741 ttatgccctt actgccgatg aaattccttc ttctgataaa gctctttttc ttgggaaagg
    13801 ttttagatgg acacagacac atacccaacg tctttttatg gttcgccagc ctgagaacga
    13861 cggacttcgt aacccctcaa aactctataa ggcagcgcga aaacatgaga ttgagacaat
    13921 gggtacagac tggatttccc gcataactcg gaaatcggca tggtggaatc cggttgctcc
    13981 cttacctccg gtcggaggtt ctcctgaatt acatggcatt gaaattgagg aagatgatat
    14041 ttgggaagat ttgggggagc gtgttggcca caaggttgta ttgggcacaa cccgcgttgg
    14101 taaaacccga ttatgcgaaa ccatgattgt tcaggatatt cggcgggggg atgttacgat
    14161 cgcatttgat ccaaagggtg acgctgacct gatgactaga atgtatgtcg aatcgcaaaa
    14221 atcaaacagg ccgttttata tgttccattt gggtttcccc gagtacagtt gccggtacaa
    14281 ccctatcggg gattatggcc gtattaccga ggtggcgaca aggattgcaa accaactgcc
    14341 ttctgaaggt cagtctgctg ctttccgaga ttttgtatgg cgtttcgtca acgttttaac
    14401 gaaaaccatg gaaggtttat ccattaaacc aagctatgaa aatatctata agtctgctgc
    14461 aaatgtagat gaacttgcag gggattactt taaaaagata ttagataaac accaccctgg
    14521 ttgggagaaa gacttcgaca attttgatat gcccgagtct gaagcaaaaa gcgcagtcaa
    14581 aactgggcgc aacttggaca caatgaagct cggttctttc ttgaaggcca aaaagcataa
    14641 cgaacctctt attgacgcat tgggtggtat tttgtccaat gacagatcct attttgagaa
    14701 gctagtcagc tccctgtatc cattgctgga aaaactgacg actggcgaag ttgccaagct
    14761 tatcagcccg gatacagagg atttgtcaga tccaagaatc attatggact ggcgcaaagt
    14821 catggaatcg aatgccgtcg tctatattgg attggattcc ctatctgatg ccgaagttgc
    14881 agcagccgtt ggtaatgcga tgtttgctga cttaacctct cttgcgggtc agatctataa
    14941 attcggccat ggggttggac agagtggaaa aacccagaaa agaaaagtct caattcatgc
    15001 agacgaattc aatgaattaa tcggtgacga attcattccg ttattgaata aggccggtgg
    15061 tgccggctac caagtgacgg tttatacaca aacctggaaa gacgtagaag ccaaaattgg
    15121 ttcccctgct aaagcaggtc aaattggcgg caacttgaat acgatgatta tgcttagggt
    15181 gaaaactgtc gaaacagcag agatgctgac gaatcagttg ccggaagtca aaattatgac
    15241 cagcactcta gtctcaaggg ctggtgatgt tgccttccct gatagtcacg aagattttag
    15301 cagtggaaac gaagacagga ttggtgctga gacagttcct atgctgacac ccgccgattt
    15361 aacccaatta cccaaaggtc aagcattcgc gcttattgaa ggtggccagc tatataaaat
    15421 tcgactccct ctacccaaaa aggacaagca ggaggaaatc cctgcgcata ttggagaaat
    15481 ggcggctgat atgcgcgaag tttacagact aagccggcct tcggctgaag aaaacgcatt
    15541 tacggaggga agtagttatg ccgtctaata caagcgcatg gtacatgaat tcattgcctt
    15601 taaagttctt atttctcccg tttaaagctg cttatacctt attcttaatt gtgctgctta
    15661 tctttggtag ttcggtgttg acacaaaact attttggtac cggtaatcta ttggaagatg
    15721 aaatcaaatg gcctgagtct gttggccatg atgcgtttcc aggaaaagag gttgacgata
    15781 ctataaatct gcaaattgcc agacttagtt acaaggggct gaaaaccgta ttttttgacc
    15841 tgacattagt caatgaatca ctaaaagcca agtcagaagc cgattttggt tacaaattta
    15901 aaaattattt agtcccccgt atgaaatatc tacagcaatt cgatactacg ttgaaggtta
    15961 tttccttacg tttgggaaat ttggcattaa tcgtgggtat ctcagtaatc tttgcaattc
    16021 cggttttgat agatggttta gtaatgagag ccattagaca ggaaaatgcc tcgcgagaga
    16081 gtgcaggcat ttatcaccgt gcaaaatact ggagaacagg gattatttgg ttgggttgca
    16141 tgatttatat gtgtgtgcca atatcaatac cgccctactt gctctattta cctctggtag
    16201 cagcaatttg gatgattttc atgcaagcta aatatcttaa gaaatatctc taggcttcca
    16261 gtagaatcta aatagtgctt ttttagttca ttaaacgggg gcttaatgct ttaactgacg
    16321 gtacagaaat ctcgaaaagc attttgcaaa atacttaaaa taagagtata gtaaaaatga
    16381 aatatttcaa gtttaactaa tagttaacaa tctagagtaa actatcgtaa tcgttaaatc
    16441 tgggtataga ttgcataata tacattatgc gaactactgg aaacagctgt atgtgttgcg
    16501 ttgaccttca atacgatata caggcagcag gcacacagga tatgccgccc atactttttt
    16561 aatccaaaac attatatcaa cgggcgcaaa cagtaaatcg cccaacttat cccaaaaatt
    16621 ctcctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
    16681 ccttacatac gtcccaattc tctttgcagt gcttggatat tttgctgtct gtccaaaaca
    16741 ttgctttgca atgcgttgat tttttgatgg ttgatgttgc cgccttttgc cagacgtgct
    16801 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cattgcttaa ttctgcttcg
    16861 agaatggagc gtctgctgtt atttacaggt gcttgttgag gcggcgtatt ggattttgcc
    16921 ggcttggata ctgttttgac cggggcttta tatttgacag cctgtccgcc gtttgacggt
    16981 gatggtgccg gttcgggagt ttggggcggg atatagcgtt cgctgctgta gttgccgatt
    17041 gggggcaaat cggttgagtg gcagctttta gacggcttgg tggtgtaaac ggtttctccg
    17101 ttgattgtgc aggtatagat tttggccgca ttcgcaccca atgggcttga aatcagggaa
    17161 aggttgatta ggattaaggg gagttttgat ttcataatgt gtttgatatt cggataataa
    17221 tttgattttt tgaggatttt ttgtaaggtt ttcttggcgt tttatgggct tttcttttcg
    17281 gttgtttatt tttgattttc cttcggggct ttggcagcct gtctgttctt tttcccggca
    17341 tgttttttgt ttttcttttt gtttcctgtg tgtattttgg cttcttgact aagtttttta
    17401 attttcaggc ggtatccgcc tccctgatgg ctgctgattt taggtaaatc agcatcggca
    17461 cacgatcctg acgggtctga tgtatatacg cttttgccat ttgagttgca atggtatatg
    17521 gaggcttgcg ccgccgcgcc ggaaagggac aggagaccca aggcggcaaa aagtctgatg
    17581 tttataccgg taaagtaaaa aggcggtggt aacgtccgaa ttataccgtt attggcaggc
    17641 aaagataagc accctgcccg cgcttcttta tcgctcggca aactgtttct cgatttcagt
    17701 ttcaatccgt tccaattctt cttgacaagc cagccaagtt tcttcgattt gggcaagctg
    17761 tgttttgact tttgccagct cggataaggt attttgcaat ttttctttgt ttgcttcaag
    17821 gtaagcgtct tcttgtgtta aaaatgcttc gcatcccgtc tgaatttcag aaagctgcac
    17881 catttctttt tcggctttgt cgattttctg ttgtatcggc ttactgcgtc gggctttttc
    17941 ttgacggatt tgcgcttcga tacgcttggt atccttgcgg ttttggcttt gagtggaagc
    18001 ggctggcgcg gttgcggcgt tttcctgtgc caagcgccat tggcggtaat cgttcaaatc
    18061 gccgtcaaag tttttcagac gacctttgtc tatcaagagg aaactgtcgg tcgtggcttc
    18121 aagcaggctg cggtcgtgcg atacgacgat taatgcacct tggaaacttt gcaacgcgag
    18181 cgttaaggcg tggcgcatat ccaaatccag atggttggtc ggttcgtcaa gcagcagcag
    18241 gttcggcttt tgccagataa tcatggcaag ggcaaggcgg gctttttctc cgccggaaaa
    18301 aggttcggtt ttctgcaacg ccatatcgcc gacaaagttg aagccgccga ggaaatttcg
    18361 gatttcttgt tcgcgtactt cgggagaaag ctgctgtata tgccaaacag ggctttggtc
    18421 ggcgcggatg gtgtcaagct ggtgttgggc gaaatagccg atattaagtt tttcggaatg
    18481 gacgatgctg ccggagagta aatcgatttt tcctgccaaa gctttgataa acgtagattt
    18541 accgctgccg ttgacaccca atagcccgta gcgtgcgccg ctttccagcg acagggtaat
    18601 gtcatgcagg acggttttac cttcgtaacc caaatcagcg tgctccagct tcagcaaagg
    18661 attgggcaga tggtcgggat tgtaaaactc aaaggaaaac tcgctgtcca gatgtgcggg
    18721 agcgatgcgt tcgagcttcg ccaacgcctt catgcggctt tgagcttgaa cggctttagt
    18781 ggctttggct ttgaagcggt cgataaagga ttgcagatgt ttgatttgcg cctgctgttt
    18841 gacatatgcg gcttgttgtt gcgccagacg ctgcgcgcgt tcggtttggt aaaaatcgta
    18901 attgccgctg tattgcgtga gcttttgctg cgacaattca atggtttggg tggtggccgc
    18961 attgaggaaa tcacggtcgt gggaaatgat gatttgcgtg cagggtaaag aggcaaggtg
    19021 gttttccaac cacaagacag tttccaaatc caaatggttg gtcggttcgt caagcaagag
    19081 caaatcagcg cggcaaatca gggcttgcgc aagattcagg cgcatacgcc agccgccgga
    19141 aaaggatttg acgggacggc tgtgttcttc ttgcgaaaaa cccagcccgt tcaacaattt
    19201 tgccgcgcgg gccggcgcgg tataggcgtc gatttcttcc aatttggcat gatattccgc
    19261 ctgcttcatg ccgtcatttt gcacttccgc ctgcctcaat gccgtctgaa aagcctgcaa
    19321 ctcggcatcg ccctgcaaaa cgtaatccaa agcggaaata tccaaatcgg gcgtttcttg
    19381 ggaaacggaa gcgagccgcc agtttttcgg aatcgagata tcgccgccgt cctgagtgat
    19441 ttcgcccttg attaaggcaa acaggctgga tttgcccgtc ccgttcttac cgatcaaacc
    19501 gacgcgctga ccgggattga cggcagcgga agccttgtcg agcaggactt tcaaaccgcg
    19561 ttgcagggtg aggtttttga tttcaatcat aacggaaaca tcgtcgggcg ggaaaagccc
    19621 gtattttacc tgaaagtcag tgccgatgcc gtctgaaacg ggaaatttac ggctgaagcc
    19681 aagcccaagc cctgcgccct tccgagtgca ggaaaaccaa tgtcctgaat gccgaatcgg
    19741 tattcatgca ttccacgctg attccgattc gggaaaaatc cgccatgatt ttgggatgga
    19801 taaactcctg agccgcgccc gtcccgataa tcaatatttc cggatagtca acaggtttga
    19861 tgtcggacaa caggttttcc ggagtcaggt cggacaaggt tcggcattgc ggcaggcaga
    19921 ccgaatcctt atgtacaagc acgggtttat ggaaactttg ccccgccagc cggattccgc
    19981 ccgcgccgca ttcatattct gcaaactgtc cgtctatcgg attttcttca aacaacattt
    20041 tttaccccgt tgctgcatca tctgcaccga aaggggtgca aaatcaggcg aattaatgta
    20101 ggattggcag atttcatctg acgcgcctgc cgatttcaga cggcatttga ttcaaagtgc
    20161 ggcacaatta taccggcagc ggatattttc gtctttcaat atttacattt cagtcggctt
    20221 acaaggagac acaatgaagc cagtaaacat cggtctttta ggcttaggta cggtcggcgg
    20281 cggtacggct gccgtgttgc gggacaacgc agaggaaatt tcccgccgct tggggcgcga
    20341 aatccgtatt tctgccatgt gcgacttaag cgaagaaaaa gcccgccaaa tctgcccgtc
    20401 cgccgccttt gtcaaagatc cgttcgaact ggtcgcgcgt aaagacgtcg atgtcgtcgt
    20461 cgaattgttc ggcggcacgg gcattgccaa agaagcggtg ttgaaagcca ttgaaaacgg
    20521 caaacacatc gttaccgcca acaaaaaact gctcgccgaa tacggcaacg aaatcttccc
    20581 gctggcggaa aaacaaaacg tcatcgtcca atttgaagcg gcagtagcgg gcggtatccc
    20641 aatcatcaaa gccctgcgcg aaggtttggc ggcaaacagg attaaatcca tcgccggcat
    20701 tattaacggc accagcaact tcatcctctc cgaaatgcgc gaaaaaggca gcgcgtttgc
    20761 cgacgtattg aaagaagcgc aggcattggg ttatgccgaa gccgacccga ccttcgacat
    20821 cgaaggcaac gacgcaggcc ataaaatcac catcatgagc gcgctggcat tcggtacgcc
    20881 gatgaacttc tccgcctgct atctcgaagg catcagcaaa ctcgacagcc gcgacatcaa
    20941 atacgccgaa gaactcggct accgcatcaa actgttgggc atcacccgca aaaccggcaa
    21001 aggcatcgaa ttgcgcgtcc atccgaccct gattcccgaa agccgcctct tggcaaacgt
    21061 cgacggcgtg atgaacgccg tgcgcgtcaa cgccgatatg gtcggcgaaa ccttatatta
    21121 cggcgcgggc gcgggcgcat tgccgaccgc ttccgccgtg gttgccgata tcatcgacat
    21181 cgcccgcctg gttgaagccg acaccgccca ccgcgtaccg catctggcgt tccaacccgc
    21241 ccaagtccaa gcgcaaacca tcctgcctat ggacgaaatt accagcagct actacctgcg
    21301 cgtccaagcc aaagacgaac cgggcacgct ggggcaaatc gccgcgctgt tggcacaaga
    21361 aaacgtgtcc atcgaagcct taatccaaaa aggcgttatc gatcagacca ctgccgaaat
    21421 cgtgattctg acccacagca cggtcgaaaa acacatcaag tcggcaatcg cagccatcga
    21481 agcactggat tgtgtggaaa aaccgattac catgatccgc atggaaagcc tgcatgactg
    21541 agccgaaaca cgaaacgccg acggaagagc aggttgccgc gcgcaaaaaa gcaaaagcca
    21601 aaatccgcac catccgcatt tgggcgtggg tcattttggc gttgctcgct tcaaccgccc
    21661 tgctctccca atgcgcgatg tccaaaccgc aggcaaaaca gaaaattgtc gagtcttgca
    21721 tgaaaaatat tccgtttgct gaaaaatggc agaacgattt gaaagcgcgc ggcttggatg
    21781 cggacaatac ccgtctcgcc gtcgactact gcaaatgtat gtgggagcag cctttggacg
    21841 gattgagcga gaaacagatc agctccttcg gcaaactcgg tgcacaagaa cagcttgacc
    21901 tgctcggcgg cgcaaacgcg tttgaaactc gagacaaaca atgtgtcgcg gatttgaaag
    21961 ccgattgaac ctcaggtctg gaatgccgtc tgaacgcggg gaaaagcgtt tcagatggca
    22021 tttttccatc cgggtttgac gggcagccct tcatccgaat ccgacgggca aacccctatc
    22081 ctgattcggc ggggaaaaca aaaagccgat tcttcaagga atcggctttt gctgaaaaac
    22141 agtttacagt gcgtctttca atgctttacc tgcacggaat ttaagcgttt tggcggcggc
    22201 aatggtcaga ggctcgccgg ttttggggtt gcggccttgg cgttccgcac gttcgccgac
    22261 gtagaaagta ccgaaaccga ccagagtaac ggtgtcgcct tgtttcaggg cgttggttac
    22321 tgcgttggta gtggcatcca aagctttttg tgcggcggct ttggaaatat cggcttcttg
    22381 agcaatcgct tcgatcaatt cagacttgtt cacaatcagt cccttcctgt cttaaaaaat
    22441 gatgaaatgc ccgaatactc ggggtttgta ctgcttgagc aactttcgct ttatagcaat
    22501 tctgaaattg ccgtgtcaag caaaaaatac ggaatcgccc tattttacag gctttcagga
    22561 cgaaaccgca tttttacaac acatttcctg cgtttcaatg tttgattgcc ctgctgcggg
    22621 gtttcggttt tgaagcggat tccgccgccg cttccgcggc agaaggttct gcccaagact
    22681 caggccggct ttccaaaccc aaagccaata cctcgtctat ccatttgacc ggatggatgg
    22741 ttaatccggt tttcacgttt tcagggattt cttccaagtc tttgacattg tctttcggaa
    22801 tcaggacgtg tttgatgccg ccgcgaagcg cagccaacag tttttccttc agaccgccga
    22861 tcggcaggac ttcgccgcgc agggtaattt cgcccgtcat ggcaacatcg gcacgcaccg
    22921 gaattttggt aaaggcggac accatcgcca aagtcatagc aatgcctgcg ctcggaccgt
    22981 ctttcggcgt tgcaccttcg ggaacatgga catgaatgtc ttttttctcg taaaaatcag
    23041 gagccaaacc tactgattcc gcacgggaac ggacaacaga ccatgcagcg gatacagatt
    23101 ccttcatcac atcgcccaac tggccggtac actggatcat gcctttaccc ggtaatgctg
    23161 cggcttcgac agtcagcaat tcgccgccga cttccgtcca agccaaaccg gtaacctgtc
    23221 cgatgcggtt ttcactttcg gcaacgccgt aatcgaagcg gcgcacgccc aagtagtcat
    23281 gtaggttttt ctcattgact ttaaccgccc taggcttggc tttgctggtt ttcttggttt
    23341 cagacaacct cttcttatct tcgttcaagg taatctgcat caccacctta cggcagattt
    23401 tggcaatttc gcggtcgagc gaacgcacgc ccgcctctcg ggtgtaataa cggataatat
    23461 cgcgtaccgc gctttcttcg accaccaatt cgccttcttt cacgccgttg cgtttcattt
    23521 gcttcggtac gagatactgc atcgcgatat tgattttctc gtcttcggta tagccggaca
    23581 gacggatgat ttccatacgg tcaagcagcg gggtcgggat attcaggctg tttgaagtcg
    23641 caataaacat cacgtcgctc aaatcgtaat ccacttcggc ataatgatcg gcgaacttgt
    23701 tgttttgttc gtgatcgagc acttcaagca acgcgctggc gggatcgccc cgaaagtcgc
    23761 tgcccaattt gtcgatttcg tcgagcagga acaacgggtt tcttacgcct gcttttgcca
    23821 tattctgcaa aatcttgccg ggcatagagc cgatataggt acggcggtgc ccgcggattt
    23881 cgctttcgtc gcgcacgccg cccaaggcca tacggacata tttccgcccc gttgctttgg
    23941 cgatggattc gcccaaagag gttttgccca cgcccggagg gccgaccaaa cacagaatcg
    24001 gacctttgag cttgtccata cgtttttgga cggcgaggta ttccaaaatc cgttctttga
    24061 ctttttccag gccgtagtgg tcggcatcca gcaccagtcc ggctttggcg atgtctttgc
    24121 tgacgcggga ttttttcttc cacggcagtc cgagcagggt gtcgatgtag ttgcgtacga
    24181 cggtggattc ggcagacatc ggcggcatca ttttgagttt tttcagttcg gacaggcatt
    24241 tttcttccgc ttctttggtc atacccgcct ttttgatgcc tgcctccaag gcatccagtt
    24301 cgccgttttc gtcttcttcg cccaattctt tgtgtatcgc tttaatctgt tcgttcagat
    24361 aatattcgcg ttgggatttt tccatttggc gtttgacgcg tccgcgtatg cgtttttcgg
    24421 cctgcataat gtcgagttcg gattccagct ttgccagcag gaattccatc cgtttgccga
    24481 tttcgggaat ctccaaaatc tgttggcgtt gcgccagttt caactgcaaa tgcgctgcga
    24541 ccgtatcggt tagccggctg ttttcggcaa tgccgttgat gctgccgata atttcggcag
    24601 ggattttttt attgagtttg gcgtattgtt caaactgcgc caacagggtg cggcgcacgg
    24661 cttcgaggtc ggtattgccg cccgtgtctt cttccacgac cgcctctata tgagaaacga
    24721 acagcccgcc cgtgtcttcg atggtcaaaa cacgtccgcg atacagccct tcgaccagca
    24781 cttttaccgt gccgtcgggc agtttcaaca cttgcaggac ttgtgcgacg gtaccggtct
    24841 gatacaggtc ggtggcaact ggttcttcta ccgccgcatc ggtttgcgcc aacaggaaaa
    24901 ccggttcctc gcgggtaatg gcgttttcca gtgcggcgat ggatttcggc ctgccgacga
    24961 acagcggcag aaccatatgc gggtaaacga cgacatcccg taaaggaagg gtcgccaaag
    25021 cagcatattc ctcaaaatgc ttttcttttt gtgtcatgga tactctcttg tgtctgacag
    25081 gatacggatt gccgcgtaaa ttggggttga aagtattatt tcaagcatac gcggtttatt
    25141 tatggagttt gatgccatgc cgtctgaaac attccggctt cagacggcat gggcttggaa
    25201 agacaaggcg ggaacaaaaa aacagttctg tgttgccgct ccttgctgta ccatccgtat
    25261 ggtttgcggt tctgccgccc tattttcaaa acatgacgca ggtatcccat gtcttctttt
    25321 atttttcccg ataacgggtt cgaatggcgc aacgaaagtc tgcaaaagcc gcccaaaggt
    25381 tggtattatg tccgcgaaag cggtgcagac ggcattttga aggctaccta tcaaaatatt
    25441 gcaactgtct ggcagggcga tttccacaat gccaaacagg tgctttcggc aatgaagaag
    25501 aaggtccgca aacctgccgc cgtccgttcc gatgcggata tcgccgccct tttccacgcc
    25561 caccgtatga agcaggcgca acagagccgt attctgaata tgcttgccgt tgaaatccgc
    25621 cccggtttcg tgttggacaa caaacgcgcg cccgatatac gcgccgcttt gctcgacgtg
    25681 tacggagagg cggacggcaa accgtttttt ctgccgctca atctgctgct ggggtttatg
    25741 ggtgtgcatg agtggcataa gaaagatact actttaattg acaacagcat
//
LOCUS       DAXFHF010000026        24926 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXFHF010000026 DAXFHF010000000
VERSION     DAXFHF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24926
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.026"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(59..253)
                     /locus_tag="ACFIRM_000999"
     CDS             complement(59..253)
                     /locus_tag="ACFIRM_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC3028651.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFIRM_001000"
     CDS             complement(243..2324)
                     /locus_tag="ACFIRM_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC3028652.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLATQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="ACFIRM_001001"
     CDS             2847..3227
                     /locus_tag="ACFIRM_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC3028653.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3855)
                     /locus_tag="ACFIRM_001002"
                     /pseudo
     CDS             complement(3363..3855)
                     /locus_tag="ACFIRM_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4187..5620
                     /locus_tag="ACFIRM_001003"
     CDS             4187..5620
                     /locus_tag="ACFIRM_001003"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC3028654.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNP
                     TVRKISFTGSTEVGAKIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYVQSGIYDVFCRKLSEKAAALKLGDGLDEGLNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGAVK
                     RSGLGREGSKYGADEYLELKYLCIDAG"
     gene            5731..6516
                     /locus_tag="ACFIRM_001004"
     CDS             5731..6516
                     /locus_tag="ACFIRM_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC3028655.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6884..8440
                     /locus_tag="ACFIRM_001005"
     CDS             6884..8440
                     /locus_tag="ACFIRM_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC3028656.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARITGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFAVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8457..9203
                     /gene="surE"
                     /locus_tag="ACFIRM_001006"
     CDS             8457..9203
                     /gene="surE"
                     /locus_tag="ACFIRM_001006"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC3028657.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFYYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9305..10318
                     /locus_tag="ACFIRM_001007"
     CDS             9305..10318
                     /locus_tag="ACFIRM_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3028658.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVTAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10716..11198)
                     /locus_tag="ACFIRM_001008"
     CDS             complement(10716..11198)
                     /locus_tag="ACFIRM_001008"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3028659.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11351..11512)
                     /locus_tag="ACFIRM_001009"
     CDS             complement(11351..11512)
                     /locus_tag="ACFIRM_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028660.1"
                     /translation="MSEQPEKHHASPIEDERKNPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11586..12047)
                     /gene="recX"
                     /locus_tag="ACFIRM_001010"
     CDS             complement(11586..12047)
                     /gene="recX"
                     /locus_tag="ACFIRM_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC3028661.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12120..12830)
                     /locus_tag="ACFIRM_001011"
     CDS             complement(12120..12830)
                     /locus_tag="ACFIRM_001011"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC3028662.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13225..14444
                     /locus_tag="ACFIRM_001012"
                     /pseudo
     CDS             13225..14444
                     /locus_tag="ACFIRM_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14504..15310)
                     /locus_tag="ACFIRM_001013"
     CDS             complement(14504..15310)
                     /locus_tag="ACFIRM_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC3028663.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15502..15711
                     /locus_tag="ACFIRM_001014"
     CDS             15502..15711
                     /locus_tag="ACFIRM_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC3028664.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15800..17014
                     /locus_tag="ACFIRM_001015"
     CDS             15800..17014
                     /locus_tag="ACFIRM_001015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC3028665.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17086..19665)
                     /gene="clpB"
                     /locus_tag="ACFIRM_001016"
     CDS             complement(17086..19665)
                     /gene="clpB"
                     /locus_tag="ACFIRM_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC3028666.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALVADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            19925..20935
                     /gene="trpS"
                     /locus_tag="ACFIRM_001017"
     CDS             19925..20935
                     /gene="trpS"
                     /locus_tag="ACFIRM_001017"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC3028667.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLLAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            21016..21561
                     /locus_tag="ACFIRM_001018"
     CDS             21016..21561
                     /locus_tag="ACFIRM_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028668.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21878..22213
                     /locus_tag="ACFIRM_001019"
     CDS             21878..22213
                     /locus_tag="ACFIRM_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028669.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAAEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAAQEA
                     ADKMKDAAK"
     gene            complement(22423..23931)
                     /gene="ppx"
                     /locus_tag="ACFIRM_001020"
     CDS             complement(22423..23931)
                     /gene="ppx"
                     /locus_tag="ACFIRM_001020"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC3028670.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNKDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDSKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24093..>24926)
                     /locus_tag="ACFIRM_001021"
     CDS             complement(24093..>24926)
                     /locus_tag="ACFIRM_001021"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3028671.1"
                     /translation="NNASSEKTIEFAGRSWTKTADSLPQKAHGLRYGLGVSREINAGG
                     HHFLYGGISGGVHYWDNKDFSEQSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYNF
                     AGGFNADFSRRLGERWRLTLNAGNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDRL
                     LYGGADWPHDMTKEAEQASVRKGLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVYR
                     FPRKDHEYQANLSLRHDKISWKGFTPQLNFRYLKIGSNMKSFYTRKNTQIFMSVEKDF
                     K"
BASE COUNT         5703 a         6283 c         6914 g         6026 t
ORIGIN      
        1 ccttattata aaaatgccgt ttgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agtaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgt cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 taggatttta acgcaaatta gcacactgat atcggttttt acttggaatt tggaaaaatt
     2581 tacattcctc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 attaaatagg gcgcgtctgg ttttatagcc caagttcccg cataggttgg caacccaatc
     3601 ctcatagcgt tgccgaaatt tttcggtatc aaaaaaatct tggtcttccg aactgtcata
     3661 aacgaaagtc ctgctgtttg ccaacgcttg caagaccgtc gtgcctaaag tttcattgtc
     3721 ggtatccaat ggcaggatat ggggggatat aggtggtcta ttgccgttaa ccccaaacct
     3781 gaacatgttt gaacaatcag agcttcttca ttagcataaa aaactgccca ataattgatc
     3841 ctgtttaaaa atcattattt aatccccgta attttgactg taatgttttg acttttgcca
     3901 tactctacca cgcgttgtaa ctgcaatctt tgcttcttat atattgtacc ctatcaaagg
     3961 gctgcattac ttttctttct aaagattact atcgcataaa aagtattttt cttaaacgac
     4021 agggatgcgc ccgtgccata ttcaaacacc gcccggatgt tgcgctgccc gatcggatgc
     4081 ttcagacggc atcggaaggg tttgcagttt tggaatatga agatgataat gtccgcgaga
     4141 ttgacggcat ttgaaagcgg ttatcccgta taccggagga cacgaaatga acgaatattc
     4201 ccaattaatc aagcatcccg atatttccct ttccccgatt tcagacggca tcggggtcgg
     4261 caatccggcg acgggcgaaa ttttggcgta tgtccgcaac acgggttcgg acaagctgaa
     4321 aaacctgatt caaaaggcgg cggcagcgca aaaattatgg gcggcgaaaa ccgccttgga
     4381 acgcgccgac atattgtggc gttggtattt tttagttaaa gaaaacaaag aagcactggc
     4441 gcgtctcatg acgatggagc agggcaaaag cctgaccgag gcgcgcggcg aaatcgatta
     4501 tgcggcttcg tttgtgcgct ggtttgccga agaggcgcgg cggattgacg gcgatgtgct
     4561 gacgagtgtg aaagcgtcgc aaaaactggt tgtgttgaaa cagcccgtcg gcgttaccgc
     4621 tgcgattacg ccgtggaatt tcccgtccgc gatgattgcg cgcaaggccg cgcctgcttt
     4681 ggcggtgggc tgcgcgatga tagtcaaacc tgcatcgctc acgcctttga gtgcgtatgc
     4741 gttggctgta ctggcctacg aagcgggcgt gccgcaggat ttgcttcccg tcgtcagcgg
     4801 ccgcgcttcg gaaatcagcc atgaatttgc cacgaacccg accgtgcgca aaatcagctt
     4861 caccggctcg accgaagtcg gcgcgaaaat ttttgccggc agcgcggcgg acattaaaaa
     4921 actcagtttg gaactgggcg gcaacgcgcc gtttatcgtg tttgacgatg ccgatttgga
     4981 caaagccgtc gaaggcgcgc tggcgagcaa gttccgcaac agcggtcaga cctgcgtctg
     5041 caccaaccgc gtttacgtcc aatcgggcat ttacgacgta ttttgccgca aattgagtga
     5101 aaaggcagcc gcgctcaaat tgggcgacgg cttggacgaa ggcctgaacc aaggaccgct
     5161 gattgaggaa aaagcggtag aaaaagtcga acagcacatc gccgacgcac tctccaaagg
     5221 cgcggtctgt ctgaccggcg gcaaacgcag cgcgttgggc ggaacgtttt ttgagccgac
     5281 tgtcttaagc ggcgttacgg cgcaaatggc ggtagctcgc gaggaaacct tcgggccttt
     5341 gtgtccggtg ttccgttttg aaaccgaagc cgaagtcatc gaggctgcga acaatacgga
     5401 atacggtttg gcagcttacc ttttcacctc cgacaccgcc cgtcaatggc gcgtcggcga
     5461 agtcttggaa tacggcatgg tcggcatcaa tacgggctta atcagtaatg aagcggcacc
     5521 gttcggcgcg gtgaaacgca gcggtttggg acgtgaaggc agcaaatacg gtgcggacga
     5581 atatctagaa ttgaaatatt tgtgcattga tgccgggtga cggatgccgt ctgaatggca
     5641 gggtttcaga cggtgtcgca ttttaagcag tctctatctg ttgtacaatg cgccctgttt
     5701 ttacggttat ttttgatttg aatgtaagat atgatggaaa acggaaaaac attcccgaga
     5761 tggcgttttg ccttgaaaag tgcgggctgg cacctcttaa tcagcctatt ggttgcaggg
     5821 ctggcggcat tgctggtttt taaggtttgg tatccttatc cttatgccga gctgacgggc
     5881 ggtctgtcgc tttatcagct ggtggtggcg gtcgatatcg tatgcggccc gctgctgact
     5941 ttaattttgg caagcccgaa gaaaaagaca aaggcacgca tggtcgattt ttccatggtc
     6001 ggcatcatcc agctggcggc tttggcgtac ggtttgcaca gcgtttcgct ggcgcgtccc
     6061 gtggtggaag cgtttgaaca ggaccgcatg accattgtta cggcggcgga agtcgtggtc
     6121 gaagatttgc acaaagcccc cgaagggctg caaagcctgt cgtggttcgg catccgccgc
     6181 attgcattga aagaacctga ggatgcggat gagaagaaca agacgctgga tttgtccctg
     6241 aaaggtatcg agccgagtat gcgtcccgac cggtggctgc cgtattccga caaggaagca
     6301 gaaaaaatcc gcaaacatct gaaaccgctg aaagtcttgg cggatgcgag aaaaacgacg
     6361 gttgcggaca ttctgaaaca ggcaggtctc gccgaagggg aggagctgta ttacctgccg
     6421 tttaccagca gcaggcagaa agagtggata gtcattaccg ataaagaggg caacaccaaa
     6481 ggctacgcgc cgatagacgg cttcatcatc ccttaaccat tgggattccg cccgcactcg
     6541 aacatccgtt cttcgcggcg gtagaatcag actgtatttg agaggggcgc aacccctcaa
     6601 tcagggcaac acatagggag acgctttatg tgccgtccgt gtattgaaac atttcaaaat
     6661 aagcccatgc cgtccgaaca tcctttcaga cggcatggcg ttttgccatt gccccgataa
     6721 gctgttaaac tgtttaaatt atttcgaccg aaggtacacg cacatgaaag accccgagca
     6781 gagcagtaag cccgcccgcc gttttttgtg cgtaccgtct gttcaggcag cttgagcgtt
     6841 tcaggctgcc tttttgcgtt tgaacttgag gaacacgaaa atcatggatt tcagttggtt
     6901 ggcagaaccg catacctgga taggttttgc cacgcttttg gtgttggaag tcgtattggg
     6961 gatagacaac cttgtctttg tggcgatttt ggcaaacaag gtccagcccg cacggcgcga
     7021 ccgcgcacgg attaccgggc tggggctggc agtcgtcatc cgcatcatta tgcttgcttt
     7081 tatggcgcat atcatcacgc tgaccgaacc gctgttccaa atcggcggcc tcgccgtttc
     7141 cggcaaggac atgattatgc tcgcgggcgg tattttcctg ctttacaaag ccactaccga
     7201 actgcacgaa cgcctcgaag ggcataaccg gtttgccgtt gccgacaacc aaaaaaaaca
     7261 cgcgccgttt tggggcgtgg tcgcgcaaat cctgatactg gatgccgtgt tttccatcga
     7321 ttcggtcatc accgccgtgg cgatggtcga tcacatcgtc gtggcgatgg gcgcggtcgt
     7381 cgtcgcaatg gctgtcatga tttccgccag caaactcttg accgaatttg tggacaggca
     7441 tcccaccgtc gtgatgctct gccttggttt tttgttgatg atcggtttca gcctgattgc
     7501 ggaagccttc catttccaca ttcccaaagg ctacctctac gccgccatcg gcttctcgat
     7561 tttaatcgaa ctgtttaacc agatttcgca acgcaacagc cgcaaaaacg actacatcgg
     7621 cagctcgtgg cgcaagcgca ccgccgaaaa cgttttgggt atgatgggca tacgcgaaag
     7681 cgtgcttgct gacgcgggcg gcgaatccgt ggatgacgcg cattttgaag aaaacgaaaa
     7741 atcgatgata cgcagcgtgc tgacgcttgc cgaacgcccg attatggggg tgatgattcc
     7801 acgccgcgac atcgaacggc tggacatttc ccaaagccgc gaagaacagt atgcccaact
     7861 gcaaaacacg ccttacagcc gcctgctcgt tgtcggaaag gcgggcgtgg acgaaccgtt
     7921 gggctacatc aacaaaaaag acctgctgtc ccaactgctg gaaacaggcg gtctcgacat
     7981 tcagacggca ttgcgccagc cgctcgtcct gcccgacggc acgaccgcct tgggcgcgct
     8041 cgaactcttc cgccaaagca gcgcggatta cgctttggtg gtggacgagt tcggcgcggt
     8101 gttgggtatg gtgacgatga aagacctgtt ggaagccatc gcaggcgagt tccccgaaga
     8161 attcgagcgc gaagaagaac ccgccgttca ggggaatccc gatgaaagcc tgacggtgga
     8221 aggcgcgttg gaatatgtgg agctggcatc ccaactcaac ctgccgcagc aggaggaaga
     8281 taccgatttc cataccgttg ccgggctgat tatggaagaa ttgcaaacca tccccgatgt
     8341 cggcgatttt gccgattttc acggctggcg gtttgaagtg gtcgaaaaag aagggcagcg
     8401 catcgagcgg gtcaaaatca ccaaattgcc cgaagaataa gcatccagga taaaaaatga
     8461 acgttttgat ttccaacgac gacggctatc tggcagaagg cattgccatt ctggcgcggg
     8521 ttgcgtcgga atttgccaat gtcagggtgg tcgcgcccga gcgggacagg agcggggtca
     8581 gcaattcgct gacgctcgac cgtcctttgc agctgaaaca ggcgcaaaac ggcttctact
     8641 acgtcaacgg cacgccgacc gactgcatcc atgtcggcca gtttgccctg cccgatttta
     8701 agcccgatgt cgtgttttca ggtatcaacc gtggcgcaaa tatgggcgac gatacgcttt
     8761 attcggggac ggttgcggcg gcgaccgaag cctatctgat gggtatgcct gctgtggcgt
     8821 tttctttaaa cgatgcttcc ggacgctatt gggcaaccgc agaaaaggca ctgtggacat
     8881 tgttggcgca tttttttaaa aaaccgccgt ccgcgcccgt cttgtggaat gtcaacatcc
     8941 ctgctgtcgc accggaagat gtcaggggaa tcaagattac ccgtttgggc agacggcatc
     9001 atgagcagaa catcgtcccg tcccgcaatc cgcgcggcga acagatttat tggatagggc
     9061 cggtcggcga agtttccgat cgggaagagg ggacggattt tggtgaatgc ggcgcaggtt
     9121 ttattaccgt aacgccgctg caaatcgatt tgaccgccta tccggacatg gcggagacgg
     9181 cggccttttg gcatacggac tgaccgtttt tcaaataccg tcgggttttg cgcttgagaa
     9241 gaacgcgcat ttttattatg atataccgca ttcaggctat atatcggaac atgaaggtta
     9301 tcttatgttg aaacaaacga cacttttggc agcttgtacc gccgttgccg ctctgttggg
     9361 cggttgcgcc acccaacagc ctgctcctgt cattgcaggc aattcaggta tgcagaccgt
     9421 atcgtctgcg ccggtttaca atccttatgg cgcaacgccg tacaatgccg ctcctgccgc
     9481 caacgatgcg ccgtatgtgc cgcccgtgca aactgcgccg gtttattcgc ctcctgctta
     9541 tgttccgccg tctgcacctg ccgtttcggg tacatatgtt ccttcttacg cacccgtcga
     9601 catcaacgcg gcgacgcata ctattgtgcg tggcgacacg gtgtacaaca tttccaaacg
     9661 ctaccatatc tctcaagacg atttccgtgc gtggaacggc atgaccgaca atacgttgag
     9721 catcggtcag attgttaaag tcaaaccggc aggatatgcc gcaccgaaaa ccgcagccgt
     9781 agaaagcagg cccgccgtaa cggctgccgc gcaaacccct gtgaaacccg ccgcgcaacc
     9841 gcccgttcag tccgcgccgc aacctgccgc gcccgctgcg gaaaataaag cggttcccgc
     9901 ccccgcgccc gccccgcaat ctcctgccgc ttcgccttcc ggcacgcgtt cggtcggcgg
     9961 cattgtttgg cagcgtccga cccaaggtaa agtggttgcc gatttcggcg gcggcaacaa
    10021 gggtgtcgat attgccggca atgccggaca acccgttttg gcggcggctg acggcaaagt
    10081 ggtttatgcc ggttcaggtt tgaggggata cggaaacttg gtcatcatcc agcacaattc
    10141 ctctttcctg accgcgtacg ggcacaacca aaaattgctg gtcggcgaag gtcagcaggt
    10201 aaaacgcggt cagcaggttg ctttgatggg caataccgat gcttccagaa cgcagcttca
    10261 tttcgaggtg cgtcaaaacg gcaaaccggt taacccgaac agctatatcg cgttctgact
    10321 ttccgtttgc aatcaggtta aaaatgccgt ctgaaaggtc ttcagacggc atttttgttt
    10381 tggcggctgt gttttcaaat cattccgaag ggacggggcg tataaatttg tttgactgtg
    10441 tttgggtatt tgcttgattt catccgtttg caatggaaaa gccgcttatt tccgttcgga
    10501 gggcggtttt atcggataaa aggcattttg tccgactgat tagggctgca tcaagggaat
    10561 atgaaaacac aaccgcttag tctcacggct gtgttccaaa atggcgttga aaaacggatg
    10621 agccagaatc agccgatttt acttcaataa atcaaaccat tacaccgaat gtcggaacaa
    10681 tgcttgtcct atctttcaga cggcataggc cgccgtcagc agccgcagga cacgtctccg
    10741 gaggcttgga ggctgatttc tctgcgtttt ttggcttttt cgtagcggca gcgttggcgg
    10801 tcggtcagga tttccagcgg agggacgggg acgggtttgc cgtctttgac cgcaaccatc
    10861 gtgaagtagc agctgttggt atggcggatt tcgccggtgc ggatgttttg tgcttcgaca
    10921 cggatgccga tttccatcga ggtgcgtccc gtgtagttga cagcggcgta aaaggtaacc
    10981 aaatcgccga tgtggatggg ttctttaaac aggactttgt caaccgaaag ggtaacgcaa
    11041 taattgccgc tgtaacggct ggcgcaggaa taggcaacct ggtcgagcag gagcaggagt
    11101 tcgccgccgt gtacgttgcc gctgaaattg gcggtgtccg gcatcatcag ttcggacata
    11161 atgagttcgt gggaaggcag ttggcgttgt tgtgtcatgg tgtacctgtt ttaagaaagg
    11221 gtgttttgaa atgggtgcgg gaaaatttta gggaatgggg aagtggaatt caagctaaaa
    11281 aaagctatgg agggatagtc tgccgaaatg ggccgccgat gccgtctgaa gccttcagac
    11341 ggcattttgt tcaggaaagc caaaaacgga aaaccaagaa aaacacgagt gccagcacgc
    11401 ccgcccaaac ggcgagcatg aatccggcaa ccgcccgtcc catacggtaa accgggtttt
    11461 tgcgttcgtc ttcgatgggg gaggcgtggt gtttttcggg ttgttcggac attttgttct
    11521 caaccggaaa agttggaaac gggcgtaagg ttacgctgcc gcgcaaaaag atttaagaag
    11581 gaaggtcagc agctgtcttc ccaattttcg tcccaagcgt gtttcaatgc cgtctgaacg
    11641 gtatcggcat caaaaccgcg ataggcgagg aaacgcgcct gtttttgttt ttctttgagg
    11701 ttggcggcgg ggtgtttgaa ttttttacgc aacacggcta tggcggcttg cttttcgctt
    11761 gagcggtcgg gaagcaggtt gcggctggtt ttttcatcta tgccctgttg cgccaaagcc
    11821 tgtttcagcc tcaatgaacc gtgtttgcgg cttttgctgc ggatataggc ttcggcgtag
    11881 cgcaaatccg actgccagtt gcgttcggca aattcgttta acacgttttc caactcctct
    11941 tcgctttcgg cgtgcggtgc aagtttgcgt ttcagaccga tgcggctgac ttcttggcgc
    12001 gagaggatgt ccatcgcgcg ggcgcgtagg gatttttgcg gtttcatggc ggcggtttcg
    12061 gcacggaaag attcagacgg tatgggaaac ggattccgat gccgtctgaa cagattgttt
    12121 tattcttcgt ctttgttttt ctgcggtcgc aggttttcgt aaatttcggg cagcgcgccg
    12181 ataatccggt cggggcgggt cgtatcgtct tgcgagagca gcgtcatatc gccgtaaccg
    12241 aaggtaacgc cgacactcag gcagccggcg gctttggcgg cgatgatgtc gttgcgcgag
    12301 tcgccgacca taagcatatt ggcggcatcg atacccaaaa cttcggcggc gtgccgcagc
    12361 ggcagcgggc tgggtttttt ttcgggcagg ctgtctccgc cgagtatcag gctgaagtag
    12421 tcggcgagtc ccagttgttt tagaagctcg gcggcaagga tttcgttttt gttggtaatg
    12481 attaccagcg gaatgcccaa ggatttgagc aatgccagcc cggcttcggt ttcgggatag
    12541 gggcgggtga agacgctcaa atggtcgcgg tagtatttca tataggacac gaaacctttt
    12601 tcccacagtt cggaatcggc ttcgcggtcg cggtcgttgg tgaggacgcg gtgaaccagt
    12661 ttgccgatgc cgtcgccgac gtagctttcg accactttgg cgggcagcgg tttcataccg
    12721 agttgttcca acatcgcttc tgcggcggcg gcaaggtcgg ggacggaatc gcacagtgtg
    12781 ccgtccaaat cgaaggcgac ggcttggacg tgttcgatgg ctgcattcat aatccggttt
    12841 cctgtcgggt tgaaagggtt tattttaaca tcgtttgcac agggcttcta atgtatagat
    12901 tattaacaat cgcaacaggt ttttcgggcg gtgttttcgg attttatggc ggggtctggc
    12961 aagggtttga aatcccggcg atgttccaat taaattttta aaaaataaaa tgatgactat
    13021 atctttattt aattggacat ttgcttttcg gatgcgcgct gcttttttca gacggatgct
    13081 gtgcggcttg aaaaataaaa tcatatatag attaataaaa tcgggcttat gtcgactaca
    13141 tgctattacg ttttgtcggg aaattggtta tagtaatgag cgaaatgcac gcccctgaaa
    13201 atctttttgc gaaaaggaag caaaatgtct gatgccgttg caaaagaaac cctcaatccg
    13261 tttgaaatcg cgcgaaaaca ggtcaaaacc gcctgcgacc ggctgaaaac cgatccggcc
    13321 gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaaactcga cgacggcacg
    13381 gtcaaaacct ttaccggtta ccgttcgcgt gcgttcccat cccggtgtga atctggacga
    13441 agtcaaggca ctgtcgattt ggatgaccat caaatgctgc gttgcaggca ttccttacgg
    13501 cggcggcaaa ggcggcgtta ccttggatcc gcgcgattat tccgaggcgg aactggaacg
    13561 catcgcccga gcttatgccg aagcgattgc cccgctgatc ggcgaaaaaa atcgatattc
    13621 ccgcccccga tgtgaacacc aacggcaaaa ttatgtcgtg gatggtggat gcctatgaaa
    13681 acgtggtgaa acattccgcg ccgggcgtat ttaccggcaa accggttgag ttcggcggct
    13741 ctttggcgcg taccgaggct accggttacg gcgtgaacct cgccgccgtc caagctttgg
    13801 aaaaactggg caaagatgtg aaaggcgcaa cctacgccat tcaaggtttc ggcaatgtgg
    13861 gctaccacac cggctattac gcgcatcaat ccggcgcgaa agtcgttacc gtatctactg
    13921 ttgacgttgc tatctacaat gaaaacggct tggatatgga agcgctgttt aaagagttcc
    13981 aagaaaaagg cttcatcacc aacaaagccg gttacggcaa agaaattacc aacgccgaac
    14041 ttttggcttt ggatatggac gtactcgccc cttgcgcatt ggaaaaccag ctgacttccg
    14101 aaaacgccgg taaagtccgc gcgaaaatcg tggttgaagg cgcaaacggc ccgactacgc
    14161 ccgaagccga cgttatcctg cgtcaaaacg gcgtattggt cgtgcccgat attttggcaa
    14221 actgcggcgg cgtggtcgtt tcctatttcg agtgggtgca aaacctgcaa ggctattatt
    14281 gggagtttga cgaagttcag gaaaaagaaa tcgtcgtcct gcgccgcgcg ttccgcgata
    14341 tttggaactt ggcgcaagag tatgatgtcg atttgcgtac cgcgtcttat atgatgagta
    14401 tccgccgtgt tgaaaaagcg atgaagctgc gcggctggta ttgatgtttg aacaaaggat
    14461 gccgtctgaa accatttcag acggcatttt tgtttggacg gaattaatgg tgagaatggc
    14521 cgatttggaa ggttaaagtt gtgtagttcg cctgtttttg gcacaggctt tgatcgggga
    14581 aatcggcttt gtattcgaca ctcgctttcc aaaagccttg gcgcaagggg atgatgtcca
    14641 cttcgccttt gccgtcggtg gtgtcggaga aggcttgggc ttcggttttg tgcgttttgc
    14701 tgcggtcgct ggtgtcgaag ccgtcaaatg tagcggtaac ggtggcattg ggcagcggtt
    14761 cgccacggaa cagaacgcgg actttgaagc gttcgcctac gtgaatgttg gcgggattgt
    14821 ccagcgggac gatttccaag ttttgtccga ccggtttggt gatgatggcg gtgtccgcgc
    14881 tttcgtgtcc cacgttgaca atgtttttac cgaacatacg ggtttgttcg caatagcttg
    14941 cgtcaggcat ttctttgatg ccagcctgtt tccagcctgc tttgtttttt gaccggaaag
    15001 taggctgata ttcggcggta acgaggtagc tgccgtcttt gacgggacgg ttgctgcggt
    15061 attggtagtt gtatgtgccg cgttgaatca tgttttcctt acctttttcg gtaaccagct
    15121 gcatcggttt gctgaagatg tgcaggcggt ctttggcgat gggttcgagt tcggggaatt
    15181 cgccgtagcc caagtcggct ttaaggtatt cgccgccgtg cgtgtgggcg gtttcgaccc
    15241 agacgcggtg ggcgtgtgcg gatgtggcaa atagtgcgga aacggcgagc agtgtcaatg
    15301 ttttcttcat ggtgcagcct ttatatcgtg cgggttaaaa attttgttat gctataacaa
    15361 aatatgtatc gtgtgaagac ggatttgccg cctgaaaact gtctttttgt atcgatttga
    15421 atttttcaga aaatgaagcg gacgtttggg cggaggcaac ttgtttgata agatagcaat
    15481 atttcaaaac ggagaaatat catgccttat gtcaatatta aagtaaccgg cggcaaggaa
    15541 gcaccgactg ccgcgcaaaa agcggaactg atcggcggcg taatcgaatt gctggcacgc
    15601 gtgctgggta aaaatcccga aacaacggtt gtcgtgattg acgaagtgga taccgataac
    15661 tgggatatag gcggcaaaag cgtcagcgaa cggcgcaaag agggcaggta aaaagcctga
    15721 aaaccccgcc ctgatgcttt aaattccgcg tgaaaaagag tacattccca cccattgccc
    15781 aaaatttacg gaacacatta tggataaatt tcccaagtct gcaaagctcg accacgtctg
    15841 ctacgacata cgcgggccgg ttcacaaaaa agcccttcag ttggaagagg agggcaacaa
    15901 aatcctcaag ctcaatatcg gcaaccctgc gccgttcggc tttgaagcac ctgatgaaat
    15961 cttagtcgat gtcatccgca acctgccgac ttcgcaaggc tattgcgatt cgaaggggtt
    16021 gtattccgca cgcaaagcca ttgttcacta ctatcagaac aaaggtttgc gcgatattac
    16081 ggtaaacgat gtctatatcg gcaacggcgt gtccgagctg attgcgatgt ctatgcaggc
    16141 gttgctcaac gacggcgacg aaatcctgat tcccgcgccc gactaccctt tgtggacggc
    16201 ggcggcaacg cttgcgggcg gtacggtacg ccattatctg tgcgacgaag aaaacggctg
    16261 gtttcccaac cttgccgata tggaagccaa aatcacgtcc aaaaccaaag ccatcgtcgt
    16321 catcaacccc aataacccga caggagcggt gtacagcaag gaaatcctgc tggaaattgc
    16381 cgaactggcg cgcaagcatg gtttgattat tttcgccgac gagatttacg acaaaatcct
    16441 ttatgatggc gcggttcact gccacatcgc cgcgctcgcc cccgaccttt tgacggtaac
    16501 gttcaacggt ttgtccaaag cctaccgtgt agccgggttc cgccaaggct ggatggtgct
    16561 aaacgggccg aaacatcacg caaaaggtta tatcgagggt ttggatatgc tctcgtccat
    16621 gcgcttgtgt gccaatacgc cgatgcagca cgcgattcag acggcattgg gcggttatca
    16681 gagcattaac gaattcatct tgcccggcgg acggcttttg gaacagcgca acagagcgtg
    16741 ggaactggtc aaccagattc ccggcgtgtc ctgcgtcaaa ccgatgggcg cgatgtatat
    16801 gttcccgaaa atcgataccg aaatgtaccg catccgcgat gatatgaaat tcgtttacga
    16861 tttgctggtg cgcgaaaaag tcctgctggt acagggaacg gggtttaatt ggatcaaacc
    16921 cgaccatttc cgcattgtta cgctgcctta cgtccatcag attgaagagg cgatgggcag
    16981 gttggcaaga ttcctgcaaa cctaccatca gtaggattct gataaaaaat gccgtctgga
    17041 acggagattc ccgtttcaga cggcattttc aacagcagga acaaatcagg caaatttcag
    17101 tctgtcgccg tcggcttcta ccttgatttc gctttcgggc gcatagtttc cggcaagcag
    17161 ggctttggcc agcgggtttt cgatttccga ttggatcgcg cgtttgagcg gacgtgcgcc
    17221 gtaaatcggg tcgaaaccgg ctttggcgat gatgtccaga gcggcatcgg aaacagtcag
    17281 gcgcaggttt tgtttttcca aacgtttttc caaacctttg agctggattt tcgcaatact
    17341 gcggatattg gcctgatcca agccatggaa cacgaccact tcgtcgatgc ggttgatcat
    17401 ttccggacgg aaatgttctt tcacatcctc catcacaact tctttcaccg cttcgtagtc
    17461 ctgcgtgccc atttgttgga tgtgctggct gccgatgttg gaagtcatca caatcacggt
    17521 atttttaaaa tctaccgtcc tgccttgtcc gtcggtcaaa cgaccgtcat ccaatacttg
    17581 cagcaggatg ttgaatacgt cgggatgggc tttttccact tcgtccaaaa gaatcacgct
    17641 gtacggtttg cggcggactt gttcggtcag gtagccgccc tcttcgtatc cgacataacc
    17701 cggaggcgcg ccgattaagc gggcaacggc gtgtttttcc atgtattcgg acatgtcgat
    17761 gcggatgagg tggtcttcgc tgtcgaacag gaagcctgcc aaagctttgc acaactcggt
    17821 cttacccaca ccggtcgggc ctaagaacag gaagctgccg taaggtttgt tcggatcggc
    17881 aaggccggag cggctgcggc ggatggcatc ggacacggcg cgcacggctt cgttttgacc
    17941 gactacgcgg cggtgcaaca cttcttccat tttcaacaat ttatcgcgtt cgccttccat
    18001 cattttggat acgggaatgc cggtcatacg ggaaacgatt tcggccactt catccgcgcc
    18061 gacattatta cgcaagagtt tgtttgccgg ttttgtgctg tccgtatctg cccgttcggc
    18121 ggctgcacgc tgtttttcca aatgctccaa atcttcatac atcaattttg aagccagtgc
    18181 caaatcgcct tgccgttttg cctgttcgat tttaattttg acttcgtcaa tttgtttctt
    18241 aatattggca gcaccgtctg aaatcgcttt ttcggctttc cagatttcgt ccaaatcggc
    18301 gtattctttt tgcaagccgt cgatttcctc gtctatcagt tccaaacgtt ttttgctggc
    18361 atcgtctttt tctttttcaa cgtgcgcctt ttccatccga agctgaatca aacggcggtc
    18421 gattttgtcc atagcttccg gtttggtttc tttttccatc ttgacacggc tggcagcttc
    18481 gtcaatcaaa tcaatcgcct tgtcgggcaa aaagcggtcg gtaatgtagc ggtcgctcaa
    18541 ctccgctgcg gcaacaatgg cggggtcggt aatatcgatg ccgtggtgga tttcgtaacg
    18601 ttcctgcaaa ccgcgcagga tagcgatggt gtcttccacg ctgggttcgc caaccaatac
    18661 tttttggaaa cggcgttcga gtgccgcgtc tttttcgatg tattggcggt attcgtccaa
    18721 agtagtcgca ccgatacagt gcagttcgcc ccgcgccaag gccggtttca acatattgcc
    18781 cgcgtccatc gcgccgtcgg ttttgcccgc gccgaccaaa gtatggattt catcgatgaa
    18841 aatcagggtg ttgccgtcgt ctttcgccaa atcgttcaat acgcctttca agcgttcttc
    18901 aaattcgcca cggtatttcg caccggcaat caaagccgcc aaatccaaaa ccagcaggcg
    18961 tttgttacgc agggattcag gtacttcgcc gttgacgatg cgttgcgcca aaccttcgac
    19021 gatggcggtt ttacccacac cgggttcgcc aatcagcacg gggttgtttt tggtgcggcg
    19081 ttgcagcacc tgaatcgcgc ggcggatttc atcatcacga ccgataacgg ggtcgagctt
    19141 accgtctcgg gcgcgttggg tcagatcgag cgtgtatttt ttcaaagcat cgcgttggtc
    19201 ttcggcattc gcatcgttca cgttttgtcc gcctcgtacc gcatcgatcg cggcattgat
    19261 gttttgttcg gtcgcaccgg cttctttcaa gattttgccg gccgcatcgt tctgctgcac
    19321 caaggcgagc aggaaaagtt cgctggcgat ataggcatcg ccgcgtttgg ttgccgcttt
    19381 gtccatcagg ttcaacaccg cctgcaattc gcggctgggc agaatctcac caccttgtcc
    19441 ggacactttc ggcaggctat tcaaatgctg ctgcaaacgc tgtttaacct gcggcacgtt
    19501 cacgcccgca tgagccaaga gcgcggcagc tccgctgttt tggtcgtcaa gcagggcttt
    19561 cagtacgaaa cctgcttcca gatagctgct gtccgcaacc aacgccaaac tctgagcttc
    19621 tgcaagggct tgttggaatt tggcggttaa tttgtcgtat cgcattttcg tttccttttc
    19681 aaaatgtccg ctgtcgaagc ctatatgtgc atgactgtgg ataactcaag tgttgttttc
    19741 tgtttttcta tatttaattc gatatattgt tgaatttaaa gtatatagaa atgtataata
    19801 atatgtataa ctatatcttc ttaatatgga aaagtctgtt gtcggctgga tgtatgtggc
    19861 aaatcgggta taatcggcgc atcttttccc tttcagacgg cattgctgcc gtaaggacat
    19921 ttttatgagc aaaaaacgag ttctgaccgg cgtaaccacc accggcaccc cgcatctggg
    19981 caactacgtc ggcgccatcc gccccgccgt ccgcgcggcg caaaaccccg ataccgaatc
    20041 cttcctcttc ctcgccgatt accacggcat catcaaatgc cacgagcagg agatgattca
    20101 ccaatccacc caagccgttg ccgccacttg gcttgcctgc ggactcgacc ccgagcgcac
    20161 caccttctac cgccaaagcg acattcccga agtgatggag ttgaactgga ttctgacctg
    20221 catcaccgcc aagggtttga tgaaccgcgc ccacgcctac aaagccgccg ttcaagccaa
    20281 tgcggaaaac gggcaggaag accccgattt cggtgtggaa atgggtttgt tcagttatcc
    20341 gattctgatg actgccgaca ttttgatgtt caacgccaac gaagtccccg tcgggcgcga
    20401 ccaaatccaa cacgtcgaaa tggcgcgcga cattgccggc cgcttcaacc accgtttcca
    20461 agaactcttc accctgcccg aagtgaaaat cgacgaaaac gtcgaactct tggtcggttt
    20521 ggacggacgc aaaatgtcca aatcctacgg caataccatt ccgctttggg aaaacgacaa
    20581 aaaaacccaa aaatcggtca acaaaatcat caccaacatg aaagagccgg gcgagccgaa
    20641 acagcccgac gaaagccctt tgtttgaaat ctataaagcc ttctccacgc cgtctgaaac
    20701 ggcggaattt acgcaaatgc ttgccgacgg cttggcttgg ggcgaggcga aaaaactttt
    20761 ggcggcgaaa atcaacgccg aattggcaga actgcgcgaa cgctacaacg cattgacctc
    20821 taatccttcg caaatcgaag agattttgca ggcaggcgcg caaaaagcgc gcaaagaagc
    20881 gcgcgaattg ttggataaag tacgcgatgc ggtcggcatc cgcccgttga aataaaccca
    20941 atgccgtccg aacctccgcc tgccacgttt cagacggcat ttcagacggc attttgaaac
    21001 catcaggagt gcggaatgct gaaaatacct tttgccgtgt tgggcggctg cctgctgctt
    21061 gccgcctgcg gtaaatccga aaatacggcg gaacagccgc aaaatgcggc acaaagcgcg
    21121 ccgaaaccgg ttttcaaagt caaatacatc gacaatacgg cgattgccgg tttggctttg
    21181 ggacaaagta gcgaaggcaa aaccaacgac ggcaaaaaac aaatcagtta tccgattaaa
    21241 ggcttgccgg aacaaaacgc cgtccggctg accggaaagc atcccaacga cttggaagcc
    21301 gtcgtcggca aatgtatgga aaccgacgga aaggacgcgc cttcgggctg ggcggaaaac
    21361 ggcgtgtgcc ataccttgtt tgccaaactg gtgggcaata tcgccgaaga cggcggcaaa
    21421 ctgactgatt acctgatttc gcattccgcc ctgcaaccct atcaggcagg caaaagcggc
    21481 tatgccgccg tgcagaacgg acgctatgtg ctggaaatcg acagcgaggg ggcgttttat
    21541 ttccgccgcc gccattattg agttattcgg acatcacgga atatatttgg atttttcaaa
    21601 ccttgcggaa aaagtccgcg ccgccgggaa actcaaaagg aggggatgtg tgttacaatt
    21661 ttccgaactg ttttcataaa atagttttcg gacgtgtttc aatatggcat tgatgccgcc
    21721 ttgttgttcg agaaaaaacc ctttatattt aatataatgg gttttaacta aacgggaaac
    21781 cgttttctct ccggccgatg ggcaaaatca gccgattgat ggaacacggt ccggttttta
    21841 agcaaaacct taatcaacaa ctcaagaagg ataagacatg aaaaaattat tgattgccgc
    21901 aatgatggcg gctgccttgg cagcttgttc gcaagaagcc aaacaggagg ttaaagaagc
    21961 ggcccaagcc gttgagtccg atgttaaaga cactgcggct tctgccgccg agtctgccgc
    22021 cgaagaagcg aaaggccaag tcaaagatgc tgcggctgat gcaaaggcaa gtgccgagga
    22081 agctgtaact gaagccaaag acgcggcagc cgaaaccaaa gaagcggtaa gcgaagcggc
    22141 taaagacact ttgaacaaag ctgccgacgc ggctcaggaa gcggcagaca aaatgaaaga
    22201 cgccgccaaa taatttgttg ccttggcaaa aatgacgaga tgccgcctgc cggcaaccca
    22261 aatgaaaccg cctgaagatt ttcaggcggt ttttgagttg tatgcccctt gttttttacg
    22321 cctgcatgac cgtgcaggga ggatgtgccg cgcatatcgg gatattgcgg acagggaaat
    22381 gccgtctgaa catcatttag acggcatttg gtttgtcaag gttcagacgg cttcaacttt
    22441 gaacggcata ttgatttttt gccattggac gctttcatat tccaatgcgt cggcaatcag
    22501 ggggtggcgt tccagccatt tactgtcaat acgcaggatg aagccgcagc tttccgtatc
    22561 cgtacgcaac tgcatatttt tcgggaaaga caggtcttgg cgcgaacggc agaacaaggc
    22621 ggcaaggcgc agggacaaaa cggcatacca caacatttcg ttgccgccga taatgccgcc
    22681 catttttttc atatcgccgc gatgaccgat gaccagttgg gcaagtatgg tctgttcctt
    22741 gcgtgaaaaa cccggcatat cggcgttttc gaggatgtag gcggaatgct tgtgatagcc
    22801 ggtatgggcg atgtccaaac cgatttcatg cagcgcggcg gcgcgtccga gatactgttg
    22861 ccacaaggca agttcttgaa ccgtaacgtt tttggcatga cagaggctgt ccataaaggt
    22921 ttgcgcggtc tcggcggtgc gtttcgcctg attgaggctg acgtggtagc ggtgttggaa
    22981 ctcggcaacc gtttgtccgc gcatatcttt gtttaagccg cgcccgatca aatcgtaaaa
    23041 cacgccgtcg cgcagggcgg cttcggttac ggtcatcctg tcgagtttca tttcctcaaa
    23101 cgccgccatc atcacggcaa gtccgccggc aaaaacttcg atgcgttccg gtttcaggtt
    23161 ttcaaatttg gcttttttga ccgaaccggc ttcgatgatg cgttcggcga gggcgcgcat
    23221 tcccttgtag gtaatgtccg cctcttgggg catttcggcg gcaagcacgt cgcggatgga
    23281 ttttgccgaa cccgatgtgc cgacggcgaa atcccaacct tcgcgcctca tatttttgct
    23341 gatgcgctgg atttcgttgc gggcggcgga aatggcggct tggaagtctt tggcggtgat
    23401 tttgttttgg aagaagcgca ggctgtaggt tacgcagccc aagggcaggc tttcggtaat
    23461 gtcgggattc agcgtcgaac cgatgacaaa ttctgtcgaa ccgccgccga tgtcgataac
    23521 cagcattttg cccccgcagg gggggagggt gtggatcacg ccggtataaa tcagccgcgc
    23581 ctcttcgcgc ccggcgatga tttcgatggg gaaacccagt gccgcttcgg ccttgggaag
    23641 gaaatctgcg atgtttttgg cgacgcggaa tgtattggtt gccacggcgc gcacctgctc
    23701 gggacggaag ccgcgcaggc gttcgccgaa ttttgccaaa cagtccaaag cctgttcctg
    23761 cgaggatgtg ctgaggtttt tctgttcgtc cagaccggcg gcaaaacgga ccatctgttt
    23821 gaaggaatcg atgactttca actgtccgtt gttgttttcg caaatctgga ggcggaaact
    23881 gttggaaccc aaatcgacgg aggcgaggac gtttgcggga ttggtggtca tggcggatac
    23941 cggtggggga aaaacgcaat gttactctga cggcgcaggc gttgacaata aatgatgcgg
    24001 ctgtttttga ttctgctcac ggatgttgcc gacggtatgt ttccgacagc aaaaaatgcc
    24061 gtctgaaaaa cgttcggacg gcatgtttgc gcttatttga aatccttttc cacgctcatg
    24121 aaaatctgcg tgtttttgcg tgtgtaaaaa cttttcatat tgctgccgat tttcagatag
    24181 cggaaattga gttgcggcgt aaagcccttc caagagattt tgtcgtgccg caacgacagg
    24241 tttgcctgat attcgtggtc tttgcgcggg aagcggtaca caattgtccc gggtgcatca
    24301 aaagtcctgc gggtatagcg caggtttgcc cgcagcccca agccgccgtc gaacgttttg
    24361 accgcgccga cacgcaaacc cttgcggacg gaagcctgtt ccgcctcttt cgtcatgtcg
    24421 tgcggccagt ccgcaccgcc gtaaagcagc cggtctttcg gcgcggaata catcagcgtc
    24481 gcgcccgcca gcggcatatg gctgccgtat cgggcggcgg tgcggtcttc ctgataatgc
    24541 ttccacatat tgcccgcgtt cagcgtcagc cgccagcgtt cgcccaagcg tcgggagaaa
    24601 tcggcattga agccgccggc gaaattgtat cggctgccgc ccaagaggtt ttgcccgaca
    24661 aacggcacgg tgccgaacga gcgcgttacc gaacggtttt gatggccgaa cgacaggcgc
    24721 aggctctgtt cgctgaaatc tttgttatcc caataatgca cgccgccgct gatgccgccg
    24781 tagaggaaat gatgcccgcc cgcattgatt tcgcgcgaca cgcccaagcc gtagcgcaaa
    24841 ccgtgtgcct tttgcggcag gctgtcggcg gttttcgtcc agcttctgcc cgcaaattca
    24901 atcgtttttt cggacgaagc gttgtt
//
LOCUS       DAXFHF010000027        24017 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXFHF010000027 DAXFHF010000000
VERSION     DAXFHF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24017
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.027"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            136..351
                     /gene="rpmE"
                     /locus_tag="ACFIRM_001022"
     CDS             136..351
                     /gene="rpmE"
                     /locus_tag="ACFIRM_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC3028672.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            615..1243
                     /locus_tag="ACFIRM_001023"
                     /pseudo
     CDS             615..1243
                     /locus_tag="ACFIRM_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            1389..3065
                     /locus_tag="ACFIRM_001024"
     CDS             1389..3065
                     /locus_tag="ACFIRM_001024"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC3028673.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASFSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     RCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            3272..3877
                     /locus_tag="ACFIRM_001025"
     CDS             3272..3877
                     /locus_tag="ACFIRM_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC3028674.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3949..4341
                     /locus_tag="ACFIRM_001026"
     CDS             3949..4341
                     /locus_tag="ACFIRM_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC3028675.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            4346..4492
                     /locus_tag="ACFIRM_001027"
     CDS             4346..4492
                     /locus_tag="ACFIRM_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028676.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(4540..4986)
                     /locus_tag="ACFIRM_001028"
     CDS             complement(4540..4986)
                     /locus_tag="ACFIRM_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC3028677.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(5118..5330)
                     /gene="rpsU"
                     /locus_tag="ACFIRM_001029"
     CDS             complement(5118..5330)
                     /gene="rpsU"
                     /locus_tag="ACFIRM_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC3028678.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5619..7469)
                     /gene="ltgA"
                     /locus_tag="ACFIRM_001030"
     CDS             complement(5619..7469)
                     /gene="ltgA"
                     /locus_tag="ACFIRM_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC3028679.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            7545..7682
                     /locus_tag="ACFIRM_001031"
     CDS             7545..7682
                     /locus_tag="ACFIRM_001031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028680.1"
                     /translation="MNYMPKLHRLWNVSDIIYSGLNLNQYGVASPCRTICTVCGFVAL
                     S"
     gene            7950..8687
                     /locus_tag="ACFIRM_001032"
     CDS             7950..8687
                     /locus_tag="ACFIRM_001032"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC3028681.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            8689..9375
                     /locus_tag="ACFIRM_001033"
     CDS             8689..9375
                     /locus_tag="ACFIRM_001033"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC3028682.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            9534..10400
                     /locus_tag="ACFIRM_001034"
     CDS             9534..10400
                     /locus_tag="ACFIRM_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3028683.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(10463..11032)
                     /locus_tag="ACFIRM_001035"
     CDS             complement(10463..11032)
                     /locus_tag="ACFIRM_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC3028684.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            11190..12050
                     /locus_tag="ACFIRM_001036"
     CDS             11190..12050
                     /locus_tag="ACFIRM_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC3028685.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            12186..12362
                     /locus_tag="ACFIRM_001037"
     CDS             12186..12362
                     /locus_tag="ACFIRM_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028686.1"
                     /translation="MNTRINFTATVGKRVSQCYVTSVISTIGVTTSDAICSGGGTNKA
                     KSQCNALLKAAGRC"
     gene            12725..13078
                     /locus_tag="ACFIRM_001038"
     CDS             12725..13078
                     /locus_tag="ACFIRM_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC3028687.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            13068..13934
                     /gene="atpB"
                     /locus_tag="ACFIRM_001039"
     CDS             13068..13934
                     /gene="atpB"
                     /locus_tag="ACFIRM_001039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC3028688.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            13991..14227
                     /gene="atpE"
                     /locus_tag="ACFIRM_001040"
     CDS             13991..14227
                     /gene="atpE"
                     /locus_tag="ACFIRM_001040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC3028689.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            14297..14767
                     /locus_tag="ACFIRM_001041"
     CDS             14297..14767
                     /locus_tag="ACFIRM_001041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC3028690.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            14772..15305
                     /locus_tag="ACFIRM_001042"
     CDS             14772..15305
                     /locus_tag="ACFIRM_001042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC3028691.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            15316..16863
                     /gene="atpA"
                     /locus_tag="ACFIRM_001043"
     CDS             15316..16863
                     /gene="atpA"
                     /locus_tag="ACFIRM_001043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC3028692.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            16888..17763
                     /gene="atpG"
                     /locus_tag="ACFIRM_001044"
     CDS             16888..17763
                     /gene="atpG"
                     /locus_tag="ACFIRM_001044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC3028693.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            17801..19198
                     /gene="atpD"
                     /locus_tag="ACFIRM_001045"
     CDS             17801..19198
                     /gene="atpD"
                     /locus_tag="ACFIRM_001045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC3028694.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            19209..19631
                     /locus_tag="ACFIRM_001046"
     CDS             19209..19631
                     /locus_tag="ACFIRM_001046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC3028695.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            19803..20699
                     /gene="glyQ"
                     /locus_tag="ACFIRM_001047"
     CDS             19803..20699
                     /gene="glyQ"
                     /locus_tag="ACFIRM_001047"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC3028696.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENAGLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            20816..22879
                     /gene="glyS"
                     /locus_tag="ACFIRM_001048"
     CDS             20816..22879
                     /gene="glyS"
                     /locus_tag="ACFIRM_001048"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC3028697.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAGQMNAVADI
                     ALLGE"
     gene            22943..23980
                     /locus_tag="ACFIRM_001049"
     CDS             22943..23980
                     /locus_tag="ACFIRM_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3028698.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSGGGYIARTDADDIAAPG
                     WIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHKHGKIWKKPTRHEDIAAFFP
                     FGNPIHNNTMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSKLGRLAYYPEALVKYRLH
                     ANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLP
                     EEDFERARRFLYQCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQA
                     RSDSAGKEQEI"
BASE COUNT         5641 a         6352 c         6279 g         5745 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatacat tttatcgaat atcctcttga cgaaacaaca
       61 gaaaatagcg agaatgccga gcttgtctga ggcataggta catcccctat gcccgctttt
      121 ttgaaaggaa atcaaatgaa acaaggtatt cacccgaact accgcgaagt taacgttacc
      181 tgctcttgcg gcaacaaatt cgtaaccaaa tccgcaatgg aaaaagaaaa ctttaacatc
      241 gaggtttgct ccctgtgcca cccgttctat accggcaccc aaaaaatcgt cgataccacc
      301 ggccgcgtgg acaaattcaa caacaaattc ggcaacctgt tcaaacgctg attgccgctt
      361 tgcaagaatt aagccctgcc atcatttcga tggcagggct ttttaatatt tggcacactg
      421 acttcttatc tatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagaccg
      481 tacaaatagt acggcaaggc gaggcaacgc cgtaccggtt ttgttaatcc actatatttt
      541 gacggggttg aagttgacat ctacaaagtt taatgtttga atacattaaa aaatatattt
      601 gaatgggatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat tctgggacag
      661 ccgtagactt acttacttat cctaatgtta ttttttgcca aaaaaaagca gaaaagacat
      721 cattaacatc tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct
      781 ttttgcattt gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat
      841 tccgattttc ctaggcctca aagttttgct gttaggagat tccgatggag agtctattgc
      901 caaagagggt ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt
      961 tgcaagttgt ggcgctgaca atattggtgt ctttgtccca tattttacta ccttaaattt
     1021 agcgaatttg atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc
     1081 tgcccaaaaa ttggcacaag tcccttctgt cggagaaact ttggaaaaat atagcagatg
     1141 gtttgttgcc gttgtttatt taggattggg gatatatatc ctgattgaaa acaacagttt
     1201 tgatatgcta tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca
     1261 aagactgccc cgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag
     1321 cagtctctgc acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc
     1381 tcacgcttat gctgacctat accccgcccg atgcccgccc gcccgccaaa acccacgaaa
     1441 aaccgtggct gctgctgttg atggcgtttg cctggctgtg gcccggcgtg ttttcccacg
     1501 atttgtggaa tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
     1561 ccaccccctt ggttgcccat ctgttcggtc aaaccgattt cggcataccg cccgtgtatc
     1621 tttgggttgc cgccgcattc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
     1681 ccgcacgctt tgcaggcgta ttttttgccg ttatcggact gacttcttgc ggctttgccg
     1741 gtttcaactt tttgggcaga caccacgggc gcagcgttgt tttaatccat atcggctgta
     1801 tcgggctgat tccggttgcc catttcctca atcccgccgc cgccgccttt gccgccgccg
     1861 gactggtgct gcacggctac tcgctggcac gccggcgcgt gattgccgcc tctttcctgc
     1921 tcggtacggg ttggacgttg atgtcgctgg cggcagctta tccggcggcg tttgcgctga
     1981 tgctgcccct gcccgtgctg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
     2041 cggcagtcgc ctcgcttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
     2101 caaaaacgca gcccgcgctg tttgcgcaat ggctcaacta tcacgttttc ggtacgttcg
     2161 gcggcgtgcg gcacattcag acggcattca gtttgtttta ctatctgaaa aatctgcttt
     2221 ggttcgcact gcccgcgctg ccgctggcgg tttggacggt ttgccgcaca cgcctgtttt
     2281 cgaccgactg ggggattttg ggcattgtct ggatgcttgc cgttttggtg ctgctcgcct
     2341 ttaatccgca gcgttttcaa gacaacctcg tctggctgct gccgccgctt gccctgttcg
     2401 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc ttttgtcaac tggttcggca
     2461 ttatggcgtt cgggctgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg
     2521 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca
     2581 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     2641 cccggaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     2701 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     2761 ccgtcgtccg gagtatggag gcatcgtttt ccccggaatt aaaacgggag ctttcagacg
     2821 gcatcgagtg tatcggcata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     2881 acggcacatt gccgcaccgc gtcggcgatg tccgttgccg ctaccgtatc gtccgcctgc
     2941 cccaaaacgc ggatgcgccg caaggctggc agacggtctg gcagggtgcg cgcccgcgca
     3001 acaaagacag taagtttgca ctgatacgga aaatcgggga aaatatatta aaaacaacag
     3061 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt
     3121 tatatagtgg attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     3181 tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa tccactataa atcagccgtt
     3241 ttgcaggcat cacacaggag cgacaattat tatgatgacc ctctattccg gcattacctg
     3301 ccccttcagc caccgctgcc gcttcgtttt gtacgaaaaa ggtatggatt ttgaaatcaa
     3361 agacatcgat atttacaaca aacccgaaga cctcgccgtc atgaacccgt acaaccaagt
     3421 ccccgtgctg gtcgagcgcg atttggtgct gcacgagtcc aacatcatca acgaatacat
     3481 cgacgaacgc ttcccccatc cgcagcttat gcccggcgat cccgttatgc gcggccgggg
     3541 gcggctggtg ctgtaccgta tggaaaaaga attgttcaac cacgtccaag ttttggaaaa
     3601 ccccgccgcc gccaacaagg aacaggcaaa agcgcgcgaa gccatcggca acggcctgac
     3661 gatgctttcc ccttcgttca gcaaaagcaa atacatcctc ggcgaagatt tttctatgat
     3721 tgatgttgcc ctggccccgc tgttgtggcg gctcgaccat tacgatgtca aactgggcaa
     3781 aagtgccgcg ccgctgctca aatacgccga gcgtatcttc cagcgcgaag cctttatcga
     3841 agcactgaca cccgccgaaa aagccatgcg caaataagtc cgaaatgctt gcaaaacccg
     3901 ccgttttgca ggcattttca cattcaggca caccacacgg aacccatcat gcccacctca
     3961 accaaaccct acatcctccg cgcactttgc gaatggtgca gcgacaacag cctcacgccg
     4021 cacatccttg tctgggtaaa cgagcatacg cgcgtcccca tgcagtacgt ccgcgacaac
     4081 gaaattatgc tcaacatcgg cgcaaccgcc acgcaaaacc ttcgaatcga caacgattgg
     4141 atcagctttt ccgcccgttt cggcggacaa gcgcacgaca tatggatacc tgtcggacac
     4201 gtcctcagcc ttttcgcacg ggagaccgga gaaggtatgg ggtttgaatt ggaggagtac
     4261 cgccccgaca cgccgtctga aaacacctct gccgaaaccg cgccccggcc cgccaaaaaa
     4321 ggtttgaaat tggtcaaata aatctatgcc gtctgaacgg aatcgtgttt cagacggcat
     4381 tttgtccgat ggggcgcaaa cggaatccgt ttatcggcaa aacctgtttc ggcgtatcga
     4441 aaccgtgttg cccctgccga ttcgatattg ggacattgaa aatgccgtct gaacctgcga
     4501 tacgggcttc agacggcgtt ttgtccgata ttcgggcaat caggcggtca gtacggtttt
     4561 caagattttg ttgacttcgc ccatatcggc tttgccggcg aggcgggttt tcaatacgac
     4621 catcactttg cccatatccg ccatacctgc cgcgccggtt tcggcaacgg ctgcttcgac
     4681 ggcggtgcgg atttcgccgg cggagagcat ttgcggcagg tagcggtgca gcacgtcgat
     4741 ttcggcgttt tctttgtctg ccaaatcctg acggccggct tcagtgtaga ttttcgcgcc
     4801 gtctttgcgc tgtttgacca ttttggtcag gatggcggtg attttggcat cgtcggcttc
     4861 ggtgcgttcg tctacttcaa actgtttgac ggcggcattg atgaggcgga tggtgccgag
     4921 ggaaacttga tctttggcgc gcatcgcggt tttcatatct tcggtaaggc gggttttcag
     4981 gctcatgatg tcctcgctgg gatgtcggat ggaaacggcg ggtttcgatg ccgtctgaaa
     5041 agcaaaacac accgcagagc aggtcttgcg gtgtgtcttc atatcacagg gctgacctgt
     5101 aatctgtact tgaacgttta gtacattttg ggcggcagct gttggctgcg caggcgtttt
     5161 tgcaggcgtt ttacggctgc cgcttttttg cgtttgcgtt cggtagtcgg tttttcgtag
     5221 gcttcgcggg cgcgcagctc ggtcagcagg ccggtttttt ctacggcgcg tttgaaacgg
     5281 cgcatagcga cttcaaatgg ttcattctct tttacgcgga ttgcaggcat tttatttcct
     5341 ttaataaatt cggttgtttc atctgcccat catatcggtg gaaagggtag ggcagacggt
     5401 attgtaggtt ttccgtatcg ctgtgccttt cggcggcggg atacgcgccg tgacggaaac
     5461 atcacctcct aacgggccgg cttgtgccgt gtcaagctgc ttgaaaaagt aggaaaaaca
     5521 attttcggat tgtcttatat ttatggttcg ccgtcaatgc cgttcgggat aaaaatgccg
     5581 tctgaaagac cgggcgggtt tcagacggca tcggcacgtc agcgtgcggg aacggtgccc
     5641 atacgctgtt tgagcgggat gtgcggcgcg ccgaagaggg aggcgtagta ggcggcattg
     5701 gtcatcactt ttttgacata gtcgcgcgtt tcggaaaacg ggatggtttc ggcatatacc
     5761 gcgccttcga ggggtgtgtc cgcctgccat cggcgcgctc tgccggggcc ggcgttatag
     5821 cctgcggtgg cgaggatttc gttgttttgc aggcggcgtt tggtgtccgc catataccac
     5881 gtccccatac ggatattgcc gtcggcggtg tagagctggg cggcatccat accgattttg
     5941 ccggcgattt cgcgcgcggt ggcgggcata acctgcatca gcccctgcgc gcctacgcgg
     6001 gattgcgcgc ctataacgaa gcggctttcc tgacgaatca ggccgtaaac ccaagccgga
     6061 tcgacattaa cattttgcgc gtggcggatt acggtgtctt taaacggcga aatgtagcgc
     6121 aatgtgtagt tgagtttgcg gtcggtgcgt tccgcgctgt tgaccgccat atcgtaaaaa
     6181 ccgtggtcga acgcggtttg cgcggcggcc agcagcttgt cttcgtcaaa gccgcgtgtg
     6241 gcgaaacgcc attccgcctg agcctgacgg cgcattttcg catcgccggc ggttcggctg
     6301 tttcggaaca gcaccagcgc gcgcttaatc gcgccgtctt ccgccatacg gaggacactg
     6361 tttttgccgg catcgggcac attgttgcgc gtatcgattt tgcgccccaa ttcttcccct
     6421 gccagtacgg cataaaaatt cctgcccgtc gctgccgcct gtttgtaaag tttttccgct
     6481 tcttgcgtgt tgcccgttgc ggcgcggctg cgtgccagcc agtagagcca ggtcgggctt
     6541 ttttgcagtt tttcgggcat atgcgagatc acggaagcca gctcgtccca acgtcgggcg
     6601 cgcaaggcgg cgcgggcgta ccactcgatt tggtcgtcgg tcagttggcg gcggtcggca
     6661 accttgccgt aatagtccaa ggcggcaggc acattgaggc tttgcgactg ataatgcccc
     6721 aatacgcccc acgcgaaact gcgttgttcg gggcttaaac cgctttccat ttcggacagt
     6781 agggcggcgg cattcggcga tttgcgtgct tctttgccga tgacgttcaa cagggcatat
     6841 tcgcgcgaac cttgtgtgcc gccgtcaaac gggctgccca atgcggcggc aaggttgcgc
     6901 gcgtccgtcg tttggcggcc ggccagcagt ccgcgcacgc gcctccaggc atcgttgccg
     6961 tccaacaagc cggatgcggc tgcctgttcc aacagtttgg tgcagcccga aggcagtttg
     7021 cccgtatttt taactagttc ggcggcacgc gtatagtcgt tgcggctcga atcggcgtag
     7081 cattcgactt cttgggcgcg cccttcaggt ttgagtttgg catattcctg tgcaaacagc
     7141 gtccactgtc tgcgcgcgcc caaagacttc agccactcgt tgcggacatt ttccgccatc
     7201 gcgctgtcgc cggcgttttc caaataggcg gcggctgccg catcgttgtt ctgtttcact
     7261 gcatccagtg cggacgggta gccgccgtaa tctgccagcg tttttccttc cggttcggca
     7321 gggcgggtgg gaacgcttgc cgaaaggtcg gcagtttcta tattgtctgc cggggtcttg
     7381 ccggctggca gtgtgtttgt cgaagaacac gcggcaagca ccagggccgc cagcagcggc
     7441 agggaatgct tcatagaggg taggtacatc ggatttcctt aagaatcgga accctgaacg
     7501 gtcagggttg gaaaagacaa aatgccgtct gaacaggcgt ttgcatgaat tatatgccga
     7561 aactgcaccg cctttggaat gtttccgaca taatttatag tggattaaat ttaaaccagt
     7621 acggcgttgc ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct
     7681 gatttttgtt aatccactat atatttttca attatttgcc gttttggtgc gaaccgctgc
     7741 ctttgcccgt ttcagacggc attgtccgaa atggttgccc gcttcctgcc ttattgacaa
     7801 aaaaacgctt tcccgataat atcctacgaa aattaacctg ccgatttgac acagcttgcg
     7861 ggcataacag ctaaagcgtt ccgacaattt cagctttatc ttccgcgccc gttgtgtccg
     7921 acatcgggat ttgttgtatg ggaaagacaa tgattatttt ggacaaggtt tccaagcatt
     7981 accaaacgcg cgacaaaacc cgttttgccg ccgtcgagcc gaccagcctc gaaattcaag
     8041 acggcgaaat cttcgggctg atgggctatt ccggcgcggg caaatccact ttgttgcgcc
     8101 tgatcaacct gttggaacgc cccgacacgg gcaaagtcaa cgtctgcgga caagaactga
     8161 ccgcgctcga tgccgccgca ttgcgtcagg cgcggcagaa tatcggcatg gtgtttcagc
     8221 agttcaacct tttaagcaac cgcaccgtcg ccggcaacgt tgcctttcca ctggaaatcg
     8281 ccggatggcc gtctgaaaaa atcaaagcgc gcgttgccga gtgcctcgaa atcgtcggct
     8341 tgaccgaacg cgccggccac taccccgccc agctttcagg cgggcagaaa caacgcgtcg
     8401 gaatcgcccg cgcgctcgcg cccaagccgc aagtcatcct cgccgacgaa cccacttccg
     8461 ccctcgaccc cgccaccacg cgcagtgtct tggaatgttt ggaagacatc aacaaacgct
     8521 tcaacgttac catcgtcatc gtaacccacg aaatgagcgt catcagacga ttgtgcgacc
     8581 gcgccgccct gttggataaa gggaaagtgg tggaaatcgt cgaagtacgc ggcaaccaaa
     8641 tccacgctca atccgaaatc gggcgcgaac tgattcggga ggactgatat ggcagactta
     8701 acattcgaac aagccgtttc caccatcgtc ggcatgaaag acgaaatcgt ccgcgccttg
     8761 ggcgaaactt tcgtgatggt cggcttgtcc accacattcg ccgtcatctt cggcacgctc
     8821 ttgggcgtgt tgctcttcgt aacttccagc cgccaactgc attacaacaa gccggtgaac
     8881 ttcctgctcg acaacctcgt caacctcatg cgcgccttcc ccttcgtcat cctgatgatt
     8941 gcgatgatac ccgccacacg cgccatcgtc ggcagcacca tcggcccggt tgccgcctcg
     9001 ctggtgttga gcgtgtcggg attgttttat tttgcccgac tggtggaaca aaacctgcgc
     9061 gaagtcccaa aaggtgtgat tgaagccgcc gccgcgatgg gtgcgccgcc gattgccatc
     9121 gtcctcaaag tcctcttgaa cgaagcgcgc gcgggcatgg tttccagcat taccgtgctt
     9181 gccatcgggc ttttgtcata cagcgcggcg gcaggcatga taggcggcgg cggcttgggc
     9241 gacctcgcca tccgctacgg ctactaccgc taccaaaccg aagtcatcgt cttcatcgtc
     9301 gccctcctcg tgctgctggt catcctgatt caaagcaccg gcaacgcgtt ggcgcggaaa
     9361 ctcgacaaac gttaaacccg aatgccgtct gaacgccaaa aaccccgccg ctatccgaaa
     9421 aatgctataa aatccccctg ttcgcggcaa atgccgtctg aacgccgaat ccggacggca
     9481 ggactccctg cctgtcattt ttgtttgaaa ccgccacaac atcaggagaa aatatgaaaa
     9541 ccttcttcaa aaccctttcc gccgccgcac tcgcgctcat cctcgcagcc tgcggcggtc
     9601 aaaaagacag cgcgcccgca gcctctgccg ccgccccttc tgccgataac ggcgcggcga
     9661 aaaaagaaat cgtcttcggc acgaccgtcg gcgacttcgg cgatatggtc aaagaacaaa
     9721 tccaagccga gctggagaaa aaaggctaca ccgtcaaatt ggtcgaattt accgactatg
     9781 tgcgcccgaa tctggcattg gcggagggcg agttggacat caacgtcttc caacacaaac
     9841 cctatcttga cgatttcaaa aaagaacaca acctggacat caccgaagcc ttccaagtgc
     9901 cgaccgcgcc tttgggactg tatccgggca aactgaaatc gctggaagaa gtcaaggacg
     9961 gcagcaccgt atccgcgccc aacgacccgt ccaacttcgc acgcgccttg gtgatgctga
    10021 acgaactggg ttggatcaaa ctcaaagacg gcatcaatcc gctgaccgca tccaaagccg
    10081 acatcgcgga aaacctgaaa aacatcaaaa tcgtcgagct tgaagccgca caactgccgc
    10141 gcagccgcgc cgacgtggat tttgccgtcg tcaacggcaa ctacgccata agcagcggca
    10201 tgaagctgac cgaagccctg ttccaagagc cgagctttgc ctatgtcaac tggtctgccg
    10261 tcaaaaccgc cgacaaagac agccaatggc ttaaagacgt aaccgaggcc tataactccg
    10321 acgcgttcaa agcctacgcg cacaaacgct tcgagggcta caaataccct gccgcatgga
    10381 atgaaggcgc agccaaataa ggcagtcgta taaaatgatg ccgtctgaac cgcattccgt
    10441 gttcagacgg catttttgtc ctttaatccg ccattccctg ccattccgcc aaatccggcg
    10501 tatcgattcc gaacagcgac agcgtgtgtg cgatactgtg cgccactatg tcgtccgccg
    10561 tctgcggctt gcggtacatc gcaggaacag ggggaaacac cacgccgccc atttccgtta
    10621 cccgcttcat attgtccaaa tgggcaaggt tcagcggcgt ttcgcgcacc atcagcacca
    10681 gccgccgcct ttccttcaaa accacatccg ccgcacgcgt caagagattg tcgccgaagc
    10741 cgtgcgcgac agaggcaagc gtccgcatcg aacagggtgc gaccagcatc ccgtccgttt
    10801 taaacgtacc gctggcaatg cacgccccga tattgccgat cggatggacg aaatcagcca
    10861 aggcatatac ttcgtctttc gtataatccg tttccgaagc gcgcgccatc tccgcgcctt
    10921 tcgataccac aaggtgcgtt tcgacatctt gcgcgcgcaa aagttccaaa gccttcacgc
    10981 cgtattggaa accgctcgcc ccgctgatgc cgattatcaa acgccgtacc atcatccgcc
    11041 tttcccataa aaccgcctgc aacggcaaac cggctattat agtgaaaaaa cagaaatccg
    11101 ataaacacgg atacaaattg tcggcaacac ccaatatccg ataaaatacc cgatttaaca
    11161 tcctatctga ataggcacgg gagggcggta tggcaaaagt aaaaggcgga ttggggcgcg
    11221 gcttggattc gctgctcgcc aacggtgcgg acaacagcag cggcgaccgg ctgactgcgg
    11281 ttgcggttaa agatatccgg cccggccgct atcaggcgcg cgtccaaatc gatgacgaag
    11341 ccttgcagga actggcagat tcgattaagg cgcaaggcgt gatacagccc gtcatcgtgc
    11401 gcgaacacgg actgtcccga tacgaactga ttgcaggcga acgccgttgg cgcgccgctc
    11461 agattgccgg cctgtccgaa atccccgccg ttatcaaaac catcagcgac gaaaccgcat
    11521 tggcaatggg tttgatcgaa aacctccagc gcgaaaacct caaccccatc gaagaagcac
    11581 aaggcttgaa acgccttgcc gacgagttcg ggctgaccca cgaaaccatc gcccaagccg
    11641 tcggcaaaag ccgaagcgcg atttccaaca gcctgcgcct tttaagcctg cccgaatccg
    11701 tgcaggaaat gctttaccaa cgccgcctcg aaatggggca cgcccgcgca ctgctgaccc
    11761 tgcccgtcgt cgaacagctc gaattggcgc aaaaggccgt caaaaacggc tggtcggtgc
    11821 gcgaagtcga acgccgcagc caggccgccc ttcaaaacaa acgtcccgag ccaaaaaaaa
    11881 ctgccgccgc cgacatcggc cgcctgaatg atttgctgac tgaaaaattg ggtgtcaacg
    11941 ccgaaatcaa gaccgccaac cataaaaaag gcaaaatcat cctgcatttc gacacgcccg
    12001 aaaccttcga ccatatcttg aagcagttgg gcatagatta ccggccttaa ttttgcggga
    12061 tatgccgttt gaaatataga gaatagcttt ccagatttta agtgggaaat ataattctat
    12121 tgacattttt tctgcttcgc gtaagaatcg ttttcctgtt ttcattttta attttcgagg
    12181 aaattatgaa cacacgcatc aactttaccg caactgtggg taagcgcgtg agccaatgct
    12241 atgttaccag tgtaatcagc acaatcggcg ttaccacttc cgatgcaatt tgttcgggag
    12301 gcggaacgaa caaagccaaa agtcaatgca atgctttgct taaagcggca ggccgctgct
    12361 aatcctttat tcggaaaagg tcgtctgaaa atattttcag acgacctttt atttattgag
    12421 caaactcccc aaactgccga caatccgacc gataaactac tacaatttcg cgttgatttt
    12481 ttattatttc cacgtaaagc aaacaaatca cgtaagaaca ttaaaagtgt aaagaaattg
    12541 cggacaaact ccttaatatc ctgtaatcat atgtaatatg tgttagaata gcggagttgg
    12601 attttgggga gtcgctcgtc tgtatcggcg gcgagtttca caaagattaa catattgctt
    12661 gacgtaataa ggcttctaaa ttatattagc cgcgccccaa acctgaggcg gaaacattcc
    12721 ctttatgaag cagattatca tcctacaatc cgccgtattg tccatatgcg ccgcagttgc
    12781 ctttgccgta tggggttttg ccggattcct ttcggcggtc gggggcggtt tgtcctacct
    12841 gcttcccacg tttgttgcag ttttacttct aaaacttttc aggggaaacc ccttcctgca
    12901 aagcaggatg ttcgtcttcg gagagatttt aaaagtagtg ctgtcgctgt tgtccatgct
    12961 tgccgtattt gcaatatggc atcaatcgct ggtgttcgtc ccgtttttga tggggctgct
    13021 cggtgtcagc catctggttt ttttagtatt gttgagagtg aaagattatg gcaggtgaaa
    13081 ccattaccgc tgccgactac atcaagcacc acttgcagag cttgaccagt ttgtcggatg
    13141 ttactcaggg tcagggactg aaaaacattg ctgatttttc gtttattaac cttgatgccg
    13201 ttttttttgc cgtcctgttg ggcgtaatcg gcagcttcct gttgtggcgc ggtgcaaaaa
    13261 aagcaacggc aggcgttccc ggacgtttcc aggccgccgt ggaaatcttg ttcgagtttg
    13321 tggacgatat gtgtaagagc atcattcaca gcgaaaagtc tagaaaagcc gtcgcgccgt
    13381 tgggtctgac gctgtttgtt tggattttcc tgatgaacgc gatggatatg ctgccggtcg
    13441 atttgctgcc gatggtatgg cagggtatta ccggcaacca tcacgccctg ctgcgcatcg
    13501 taccgactgc cgatttgaac accgctttgg cactcgctgt cggcgtgttg ctgatttgta
    13561 tctattacaa catcaaaatc aaaggattgg gcggctggtt tcacgagttg ttcagtgcgc
    13621 cgttcggtgc aaaactcgca cctgcgaact ttctgttgaa cttggtcgag tttctttcta
    13681 aaaccgtatc ccacggtatg cggttgttcg gcaatatgta tgcgggcgag ctggtattcc
    13741 tgctgatagc cttgcttggc ggtgcttggg cggcttccgg cagcgttgaa gtcatggatc
    13801 cgattctgtt tgtattccac attattgccg gtttggcatg ggcgattttc catattttgg
    13861 tgattaccct gcaggcattt attttcatgg cgttggcgtt cgtctatatc gggcaggcgc
    13921 acgatgccca ctgattttcc caagtagttt gtttttgtag tttaaccttt gtttctttaa
    13981 ggagtttaaa atgggtttga ttgctatcgc atgtggtttg atcgttgcat tgggtgcatt
    14041 gggtgcatct atcggtatcg caatggtcgg ttctaaatat ttggagtctt ctgctcgcca
    14101 acctgaactg attggtccgc tgcaaaccaa actgttcctg attgccggtc tgattgatgc
    14161 cgcattcttg atcggtgtcg ccattgcact actgttcgcc ttcgtcaacc cgtttgcagg
    14221 tgcataatca ggacggattc gtccgttcgt acaggtgcag cctgtttgat taaccccaat
    14281 acgaaggtta agtaacgtga atatcaatgc aacattattc gctcaaatca tcgtcttttt
    14341 cggtttggta tggtttacca tgaaatttgt gtggccgccg attgcaaaag ctttggatga
    14401 gcgtgccgca aaaatcgccg agggcttggc tgccgccgag cgtggtaaaa gcgatttcga
    14461 gcaggctgaa aaaaaggttg cagaactttt ggcagaaggg cgtaatcagg tttccgaaat
    14521 ggttgccaac gccgaaaaac gtgccgccaa aattgtcgaa gaagccaaag aacaggcttc
    14581 ttccgaggcg gcgcgcattg cagctcaggc aaaggccgat gtggagcagg aattgttccg
    14641 cgcacgcgaa tccctgcgcg atcaggttgc cgtgttggct gtcaaaggtg ccgaatctat
    14701 tttgcgcagc gaagtcgatg cttccaaaca cgcaaaactg ctcgataccc tgaaacagga
    14761 gttgtaatct tatggcagag ttcgcaacga tcgccagacc ttatgcaaag gcattgttcg
    14821 gtctggctca ggaaaaaaac caaattgagt cttggttggg cggactggaa aaacttgcgg
    14881 cggttgttca ggaagggaag gtggcttcat tgattgaccg tcctgaaacg aatgcttcag
    14941 aaaaagcaga tatcctcatc gatttggtcg gtttgaaaga caaggagtta aaaaactttg
    15001 ttatcgtctt ggccgggcag aaacgtttgt cgatattgcc ggaagtgtat gctcaatatc
    15061 aagacttgac cttatcattc aaccatatca aatctgccgt catttacagt gcctatccgt
    15121 tgaccgacaa acaggtcggc gagttggcgc aaatgctgaa taagcgtttc gacagcgagc
    15181 tgaaaatctc tgtcgaaatc gaaccggagc tgattggcgg cataaaagtt gaagtgggtg
    15241 atcaggtttt ggatttgtct gtacaaggca aactgagtgc tttgtacacg actatgacga
    15301 attaggagag ttttcatgca gcttaatcct gctgaaatta gcgatctgat taaagccaag
    15361 atcgaaaatc tgtctgtaaa tgccgaagtg agtacccgtg gtacggtgat ttcggtaact
    15421 gacggtatcg ttcgcatcca tggtttgtca gatgcaatgc aaggtgagat gctcgaattc
    15481 ccgggtaaca ctttaggctt ggcgatgaac ctggagcgcg actccgtcgg tgccgtagtg
    15541 ttgggcgagt acgaacatat taaagaaggc gacacggtta cctgtaccgg ccgtatttta
    15601 gaagtgccgg tcggacgcga attggtcgga cgcgtcgtcg atgcattggg tcggcctatc
    15661 gacggcaaag gcccgattaa tacaactttg accgcccctg tcgaaaaaat cgcaccgggc
    15721 gtgattgcac gcaaatcggt tgatcagccg atgcaaaccg gtctgaaggc gattgactct
    15781 atgattcctg tcggtcgcgg tcagcgtgag ttgattattg gagaccgtca gacaggtaaa
    15841 acggccgtag cattggatgc catcgtcaac caaaaaggta cgggtgttat ctgtatctat
    15901 gtcgctatcg gtcaaaaagc atcttctatt gccaacgtgg tccgcaaatt ggaagagcat
    15961 ggcgcgatgg aacacacgat tgtggttgct gcaactgcat ctgaagcggc ggcattgcaa
    16021 tatatcgcac cttattcagg ttgtacgatg ggtgaattct tccgcgatcg tggcgaagat
    16081 gccttgattg tttatgacga tttgtccaaa caggctgtgg cttaccgtca aatttccctg
    16141 cttttgcgcc gtccgcccgg ccgcgaagcc tatcccggcg atgtgttcta cctgcactcc
    16201 cgtctgttgg aacgtgcggc acgtgtcaat gaacacgaag tggagaaatt gaccaacggc
    16261 gaagtaaaag gcaaaaccgg ttctctgacc gcgttgccga ttatcgaaac ccaagcgggc
    16321 gacgtatctg ctttcgttcc gactaacgtc atttcgatta ccgacggtca gattttcttg
    16381 gaaaccgacc tcttcaacgc cggtatccgt cctgcaatca atgccggtat ttccgtatcc
    16441 cgcgtaggcg gtgctgcaca aaccaaagtg attaaaaagc tgggtggcgg tatccgtttg
    16501 gcgttggcac aatatcgtga attggcggcg ttctcgcaat ttgcatccga tttggatgaa
    16561 gctacgcgca aacagttgga gcatggcgaa gtcgtaaccg aactgatgaa acagaaacag
    16621 ttcagtacgt tgaatacggc tgaaatggct ttgacccttt gggcaatcaa caacggttcg
    16681 tattctgatg ttccggttgc caaagctcta gcttttgaat ctgaattttt gagctttgtc
    16741 cgtacccaac atccggaagt tttggaagcc gtcaatgctt caggtgcaat gtccgacgag
    16801 agcgagaaaa cgcttgaagc agccatgaaa tccttcaaat cttcttacgc ctatcaggca
    16861 taaggcttga agtgaaagga gtctgaaatg gcagtaggaa aagagattct caccaaaatc
    16921 cgcagtgttc agaataccca aaagatcact aaagcgatgc aaatggtgtc aacctctaaa
    16981 atgcggaaga ctcaggaacg gatgcgtttg gcgcgtccgt atgccgaaaa agtgcgtatg
    17041 gtgatgagcc atcttgcgca aaccaatacc gatcatggta ttccgttact ggaatctcat
    17101 cgggaaatca gacgtgtcgg ttttattttg attacgtctg ataagggttt gtgtggcggt
    17161 ttgaacgcca acgtgctgaa aaagtttttg gcacaagttc aagagtatcg gaatcaaggt
    17221 attgaagagg aaatcgtatg ccttggcagt aaaggtctga tggcgtgtca gagcattggt
    17281 ctgaatgtgg ttgccagtgc cgtaaatttg ggcgataccc caaaaatgga aatgctgctc
    17341 ggacctttga cagaactttt ccaacggtat gagaaacatg aaattgacag aatccatctg
    17401 gtgtattcgg gttttgtcaa taccatgcgt caagaaccga ggatggaagt attgctgcct
    17461 atcggtgaga acgtgattgg cgattcagct cccaaatcac cgttcagctg ggaataccgc
    17521 tacgaaccga ctgcacttgc agtgttggaa tatctggttc gccgctattt agagtctgtg
    17581 gtttatcagg cgttgagcga caatatggca tccgaacagg cagcccgcat ggttgccatg
    17641 aaagctgcaa cagacaatgc aggcaatgcc atcaaagagt tgcgtctggt atataacaaa
    17701 tcgcgtcaag ctgcgattac cacggaattg tcagaaattg tagcaggtgc ggcggccgtc
    17761 tgatgccgtc tgaatcctgt acggaaatta ggatacgata atgagccaag gcaaaatcgt
    17821 acaaattatc ggtgcggttg ttgacgtgga atttccacgc gacatgattc cgcgcgttta
    17881 cgacgctttg aaattagacg aaaacggtct gactttggaa gtccaacagc ttttgggtga
    17941 tggcgtagtc cgtaccatcg ctatgggcag ctcggacggt ttgaaacgcg gcatgactgt
    18001 gagcaatact ggttcgccca ttactgtgcc ggtaggtaaa ggtacgttgg gacgcattgt
    18061 cgatgtattg ggaacgcctg ttgacgaggc aggtccaatt gataccgaca agagtcgtgc
    18121 catccaccaa gccgctccta agtttgacga actgtcttcc acaaccgaat tgcttgaaac
    18181 gggcattaaa gtgattgact tgctgtgtcc gtttgccaaa ggcggtaaag taggtctgtt
    18241 cggcggtgcc ggtgtgggta aaaccgtgaa catgatggaa ttgatcaaca acatcgccaa
    18301 agcgcacagc ggcttgtccg tgttctcagg cgtaggtgag cgtacccgcg aaggtaacga
    18361 cttctaccac gagatgaaag attccaacgt attggataaa gtagccatgg tgtatggcca
    18421 aatgaacgaa cctccaggca accgtctgcg cgttgctttg accggtttga ctatggctga
    18481 atacttccgt gacgaaaaag acgaaaacgg taaaggtcgt gacgtattgt tcttcgttga
    18541 caacatctac cgttacactc tggccggtac cgaagtatcc gcactgttgg gccgtatgcc
    18601 ttctgcagtg ggttaccaac cgacattggc tgaagaaatg ggtcgtttgc aagagcgtat
    18661 tacctctacc caaaccggtt ccattacttc catccaagcc gtatatgtac ctgcggatga
    18721 cttgactgac ccgtctccgg cgacaacttt cgcccacttg gacgcgaccg tcgtattgag
    18781 ccgtgatatt gcctctttgg gtatttaccc ggcagttgac ccgcttgact ctacttcgcg
    18841 ccaattggat ccgatggtat tgggtcaaga gcactacgac gtagcgcgcg gtgtacagtc
    18901 tactctgcaa aaatacaaag aattgcgcga catcatcgcc attctgggta tggacgaatt
    18961 gtctgacgaa gacaaactgg ctgtaatgcg tgcccgtaaa atccaacgct tcctgtctca
    19021 accgttccac gttgccgaag tgtttacagg ttctccaggc aaatatgtcg ccctgcgcga
    19081 taccattgcc ggcttcaaag ccatcttgaa cggcgaatac gatcatctgc ccgaacaggc
    19141 attctatatg gtcggcagca tcgaagaagc ggttgagaaa gcgaaaacct taaactgagg
    19201 aggtcggcat gagcatcatg caagttgagg tggtaagtgg cgagcagaaa atctattcag
    19261 gcgaggcaac atttatcgtt gttccgactg tacagggcga actcggtatt tatccgcgac
    19321 acgagccgat tatgagtttg gtgcggccgg gggctttgcg tttgaccgtt ccgggcgagg
    19381 ataaagaggt tttggttgct gtttccggcg gtgttttgga agtacagcct gataaagtaa
    19441 ctgtcttggc ggatgttgcc gtccgcagtg cggaaatgga tcgggcacgt gcggaagagg
    19501 cgaaaaaagc cgcagaagcg ggcatttccc aagctaaaga cgataaggct ttggcggaag
    19561 cacataaagc attggctgcc gcaattgcac agctcaaaac tttggactat atccgttcgc
    19621 acaagaaata aacttgtttg gtttgaaaaa gcacgaccgt ttggccgtgc tttttatttt
    19681 ccggatttaa attaaaaaat tatccatccc ctggtttcaa atcaggctgt cttcgagata
    19741 aatctattca agcggctatg gaaacagtaa aataaaacct ttattttatc gaacaataag
    19801 ccatgcttac cttccaacaa atcatcttca aactgcaaac cttctgggca gacaaaggct
    19861 gcaccgtcat ccaacccttc gacatggaag tcggtgccgg cacatcccat cccgccacct
    19921 gcctgcgcgc gctcggcccc gagccttggt ttgccgccta cgtccaaccc agccgccgcc
    19981 ccaaagacgg ccgctacggc gacaacccca accgcctgca acactattac caattccaag
    20041 tcgccctcaa acccgccccc gccaatatcc aagacctcta tctcgactcc ctgcgcgaat
    20101 tgggcatcga tcccaaagtc cacgacatcc gctttgtcga agacgactgg gaaaacccca
    20161 ccctcggtgc gtggggtttg ggctgggaag tctggctcaa cggcatggaa gtaacccagt
    20221 ttacctattt ccaacaagtc ggcggtatcg attgcacgcc cgtactcggc gaaattacct
    20281 acggcatcga acgcttggcg atgtacctgc aaggcgtgga aaacgtctat gacctcgttt
    20341 gggcaaaaac gccggacggc aataccgtaa gctacggcga cgtgtaccat caaaacgaag
    20401 tcgagcaatc tacctacaac ttcgaataca gcgatgccga ctggctgctg cgccagttca
    20461 acgactacga agcgcaagcc aaacgcctgt tcgccgaaga aaacgccggc cttgccttac
    20521 ctgcctacga gctggtcctc aaagcggggc atacgttcaa ccttttagac gcacgcggcg
    20581 cgatttccgt aaccgagcgg gcaacttata ttggacgtat tcgtgcattg agccgcgccg
    20641 tggcgcaaaa atatatcgaa agccgtgaga aactgggctt cccgttgatt aaaaaataac
    20701 gatacgtcgt ctgaagcagg ttttcaggcg acctttattg cgtatccatt aatacaggcc
    20761 gtacggcata tgcaggcttt ataagcatgg cctacggaca aacagaaagt ctcacatgac
    20821 aacccaaacc cttttaatcg aactccttac cgaagaactc ccgccaaaag ccctgaataa
    20881 tctgggcaac cattttgccg cttccgttgc cgaaggcttg gaaaaagcgc aactggttga
    20941 cggcgcggcc gaatttacgg cttatgcctc gccgcgccgt ttggcggttc aagtcaaaaa
    21001 cgtcaaagct gttcaggccg atcagaaaat cgtgaaaaaa ggccctgccg tggcgaatgc
    21061 cgtaaaagac ggtacgccga ccaaggcttt ggaaggtttt gcgcgcgggg cgggggcgaa
    21121 aatcgaagac ctgaccatca tccacgacgg caggcaggac gtgtacgcct acgaatacgt
    21181 ccaaaccggc agaccgttgg gcggactttt ggaagacatt atcaatcaag ctgttaagaa
    21241 gctgccgatt ccgaaagtaa tgcgttgggg cagcagcacg tttaccttcg tgcgccctgt
    21301 tcacgggctg attgtgctgc acggcggcga tgtcgtgaac gtcagcgttt tgggcctgca
    21361 aagcggcaat caaaccttgg gacaccgctt tctttccgac ggcgaaatca tcattgaaaa
    21421 cgccgacagc tacgccgcac aaatgcgcgg gcaaggcaaa gtcgtcgctt cgtttgccgg
    21481 gcgcaaagcc gcgattcaga cggcattgga agggcaggca cgccgtctga acgcgaccgt
    21541 tgccgccgat gaagccctgt tggacgaagt aaccgcgctg gtcgaatggc ctgttgtttt
    21601 ggaagccggt tttgaggaac acttcctcgc cgtgcctcaa gaatgcttga ttttgacgat
    21661 gcagcaaaac caaaaatact tcccgctgct cgaccaaaac ggcaagctga tgaaccgctt
    21721 cctgctggtt tccaacctgc aaaccgaaga cccgtcgcac atcatccgag gcaacgaacg
    21781 cgtattgcgc gcgcgcctgt ctgatgccga gttcttctac aaacaagacc aaaaagcgac
    21841 cttggaaagc cgcctgccca agctggcgaa cgtggtgtac cacaacaaaa tcggttcgca
    21901 ggccgaacgc atcgagcgtc tgcaaagcat cgccgcccat atcgccaaag cattgggcgc
    21961 ggatgctgcg gcagccgagc gcgccgcgcg tcttgccaaa gccgacttgg taactgaaat
    22021 ggtcggcgag ttccccgaac tgcaaggcac gatgggcaaa tactacgccc gcttggacgg
    22081 cgaaaccgaa gaaatcgccg aagctattga gcaacactac caaccgcgtt ttgccggcga
    22141 caagctgccc gaaagcaaaa ttgccgccgc cgtggcactt gccgacaaac tggaaaccct
    22201 ggtcggcatt tggggcatcg gtttgattcc taccggcgac aaagacccct acgccctgcg
    22261 ccgcgccgcc ttgggtattt tgcggatgct gatgcagtac ggtttggacg tgaacgaact
    22321 gattcagacg gcattcgaca gcttcccgca aggtttgctc aacgagaaaa cgccgtctga
    22381 aaccgccgac tttatgcagg cgcgccttgc cgtgttgctg caaaacgatt atccgcaaga
    22441 catcgttgcc gccgtactcg ccaaacagcc gcgccgtttg gacgatttga ccgccaaact
    22501 gcaggccgtc gccgtattca aacaactgcc cgaagccgcc gcgctcgccg ccgccaacaa
    22561 acgcgtgcaa aacctgctga aaaaagccga tgccgcgttg ggcgaagtca atgaaagcct
    22621 gctgcaacag gacgaagaaa aagccctgta cgctgccgcg caaggtttgc agccgaaaat
    22681 tgccgccgcc gtcgccgaag gcaatttccg aaccgccttg tccgaactgg cttccgtcaa
    22741 gccgcaggtt gatgccttct tcgacggcgt gatggtgatg gcggaagatg ccgccgtaaa
    22801 acaaaaccgc ctgaacctgc tgaaccgctt ggcagggcag atgaacgcgg tggccgacat
    22861 cgcgcttttg ggcgagtaac cgttgtacag tccaaatgcc gtctgaagcc ttcaggcggc
    22921 atcaaattat cgggagaata aattgcaacc tttagtcagc gtattgattt gcgcctacaa
    22981 cgtagaaaaa tattttgccc aatcattagc cgccgtcgtg aatcagactt ggcgcaactt
    23041 ggatattttg attgtcgatg acggctcgac agacggcaca cttgccattg ccaaggattt
    23101 tcaaaagcgg gacagccgta tcaaaatcct tgcacaagct caaaattccg gcctgattcc
    23161 ctctttaaac atcgggctgg acgaattggc aaagtcgggg ggggggtata ttgcgcgcac
    23221 cgatgccgac gatattgccg cccccggctg gattgagaaa atcgtgggcg agatggaaaa
    23281 agaccgcagc atcatcgcga tgggcgcgtg gctggaagtt ttgtcggaag aaaaggacgg
    23341 caaccggctg gcgcggcacc acaaacacgg caaaatttgg aaaaagccga cccggcacga
    23401 agacatcgcc gcctttttcc ctttcggcaa ccccatacac aacaacacga tgattatgcg
    23461 gcgcagcgtc attgacggcg gtttgcgtta cgacaccggg cgggattggg cggaagatta
    23521 ccaattttgg tacgatgtca gcaaattggg caggctggct tattatcccg aagccttggt
    23581 caaataccgc cttcacgcca atcaggtttc atccaaacac agcgtccgcc aacacgaaat
    23641 cgcgcaaggc atccaaaaaa ccgccagaaa cgattttttg cagtctatgg gttttaaaac
    23701 ccggttcgac agcctagaat accgccaaac aaaagcagcg gcgtatgaac tgccggagaa
    23761 ggatttgccg gaagaagatt ttgaacgcgc ccgccggttt ttgtaccaat gcttcaaacg
    23821 gacggacacg ccgccctccg gcgcgtggct ggatttcgcg gcagacggca ggatgaggcg
    23881 gctgtttacc ttgaggcaat acttcggcat tttgtaccgg ctgattaaaa accgccggca
    23941 ggcgcggtcg gattcggcag ggaaagaaca ggagatttaa tgcaaaacca cgttatcagc
    24001 ttagcttccg ccgcaga
//
LOCUS       DAXFHF010000028        23974 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXFHF010000028 DAXFHF010000000
VERSION     DAXFHF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23974
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.028"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(94..534)
                     /locus_tag="ACFIRM_001050"
     CDS             complement(94..534)
                     /locus_tag="ACFIRM_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC3028699.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(766..945)
                     /locus_tag="ACFIRM_001051"
     CDS             complement(766..945)
                     /locus_tag="ACFIRM_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC3028700.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(951..1538)
                     /locus_tag="ACFIRM_001052"
     CDS             complement(951..1538)
                     /locus_tag="ACFIRM_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC3028701.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(1560..2306)
                     /locus_tag="ACFIRM_001053"
     CDS             complement(1560..2306)
                     /locus_tag="ACFIRM_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC3028702.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSPPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(2296..3138)
                     /locus_tag="ACFIRM_001054"
     CDS             complement(2296..3138)
                     /locus_tag="ACFIRM_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC3028703.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELTYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(3256..3594)
                     /locus_tag="ACFIRM_001055"
     CDS             complement(3256..3594)
                     /locus_tag="ACFIRM_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028704.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGASKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEG
                     KCGEGKCGSK"
     gene            complement(3668..4117)
                     /locus_tag="ACFIRM_001056"
     CDS             complement(3668..4117)
                     /locus_tag="ACFIRM_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3028705.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            4457..5350
                     /locus_tag="ACFIRM_001057"
     CDS             4457..5350
                     /locus_tag="ACFIRM_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC3028706.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            5453..6556
                     /gene="prfB"
                     /locus_tag="ACFIRM_001058"
     CDS             5453..6556
                     /gene="prfB"
                     /locus_tag="ACFIRM_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC3028707.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            6635..6994
                     /gene="rnpB"
                     /locus_tag="ACFIRM_001059"
     ncRNA           6635..6994
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFIRM_001059"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7325..8782)
                     /locus_tag="ACFIRM_001060"
     CDS             complement(7325..8782)
                     /locus_tag="ACFIRM_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3028708.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(9564..13733)
                     /locus_tag="ACFIRM_001061"
     CDS             complement(9564..13733)
                     /locus_tag="ACFIRM_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC3028709.1"
                     /translation="MTDTTPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     AVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(13788..15635)
                     /locus_tag="ACFIRM_001062"
     CDS             complement(13788..15635)
                     /locus_tag="ACFIRM_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC3028710.1"
                     /translation="MHDTRTMMIKPTSLLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPECALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            16006..17235
                     /gene="sstT"
                     /locus_tag="ACFIRM_001063"
     CDS             16006..17235
                     /gene="sstT"
                     /locus_tag="ACFIRM_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC3028711.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            17383..18664
                     /locus_tag="ACFIRM_001064"
                     /pseudo
     CDS             17383..18664
                     /locus_tag="ACFIRM_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            18664..18867
                     /locus_tag="ACFIRM_001065"
     CDS             18664..18867
                     /locus_tag="ACFIRM_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3028712.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            18927..19175
                     /locus_tag="ACFIRM_001066"
     CDS             18927..19175
                     /locus_tag="ACFIRM_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028713.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(19168..19383)
                     /locus_tag="ACFIRM_001067"
     CDS             complement(19168..19383)
                     /locus_tag="ACFIRM_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028714.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(19376..19591)
                     /locus_tag="ACFIRM_001068"
     CDS             complement(19376..19591)
                     /locus_tag="ACFIRM_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC3028715.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(19794..21137)
                     /gene="argG"
                     /locus_tag="ACFIRM_001069"
     CDS             complement(19794..21137)
                     /gene="argG"
                     /locus_tag="ACFIRM_001069"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC3028716.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            21262..22062
                     /locus_tag="ACFIRM_001070"
     CDS             21262..22062
                     /locus_tag="ACFIRM_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC3028717.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            22102..23088
                     /locus_tag="ACFIRM_001071"
     CDS             22102..23088
                     /locus_tag="ACFIRM_001071"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC3028718.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            23749..23974
                     /locus_tag="ACFIRM_001072"
                     /pseudo
     CDS             23749..23974
                     /locus_tag="ACFIRM_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         5382 a         5894 c         6997 g         5701 t
ORIGIN      
        1 atggcaaaat gtcgtctgaa atgctgatat ttcagtattc cagacggcat ttttataata
       61 aggcttatgg ataaatttgt gtataagttg aaatcagagt tttttcccgc atacggtttc
      121 atattgtttt tcagttaatg tcccggacag gatgctcatc gggcggaggg aagaggctgt
      181 gtagttttgt gtggaaattt ccgtgttttt tgtatcaaat aactgtagcg aacttaagcg
      241 gtatgttttg ttgttgcagt ggatttccca ctcggcaatg gcagtcttgt atgcgggggt
      301 gttggcaaaa cgttcttgtt tcagattggt aacaactttt ttatcttgga aaatcatcag
      361 atttccgttt tttctcacac tgtctttatt aatatatgta tgaatattgc cgtttgagat
      421 tgtgccgata ttttcccatc gggcagactt gtccggtgtg ctgcaggcgg ttatcaaaat
      481 agggagtaaa acgaaagata ggaagattgg gcgcattagg gtttccttaa tcattgaaat
      541 ccgctcattc agaaagaacg ggttgggcgg gaggggtaat gccgtctgtg aaatgaggca
      601 aatatgggaa attgatgcag ccttcgtgtt ttacgtattt atatattccg tatggaggaa
      661 tatgctgctt ttgaagagtg tattatggat gaactgccga taacagtaaa tcggtttgat
      721 ttgtttccgt cagcttatgc cgtctgaagc cctttcagac ggcatttatt ttgaagttct
      781 ggttgttttt gccagtgata ttttgatggt ttgaagctgt cttttatatt cccggcaatg
      841 cgggcagaac agcaggtgca tatatatgga aatcttctcg cccggggtgg tttcccggtc
      901 ctgatgtttg gaaagaagca gggcaatatc gcggcatttt ttcataacgt ttacttcggg
      961 ttttcttggt tgaaccattt gatttgcagg cattggcgca atgattctcg ggcgcggtgc
     1021 ataatggtgt ggtagttgga cgtgctgata ccgcacattt gttgtatttc gttggatgaa
     1081 aaaccgagta tttccttcag ggtgaatacc cgtgcggtgt tttcaggcag gttgtatagg
     1141 cagctttgca gaattttttg gaattcgttg ttatttaatg atttttccgg agtgttccaa
     1201 tgttgcggct gcccttccgg cgtccaatgc ccgttttggg aaaaatggct ttcaaatgct
     1261 tcatccagca gctcgtcatc cagtgcggta aagactttcc tctgcctccc gatttgacgt
     1321 aatgcgtcaa taattttgtt tttcaatatg gcaaaaagcc agctgttgac aagtgccctg
     1381 ccttgaaaac tgtcgcctgc gctgtatgcg gacagcaatg tttcctgcac taaatcttca
     1441 gccaaatcag ggtggtcggg caactggagc cgcgcaaaat gcagaagcag tttgcgcgac
     1501 tccattaatt cggcatcggt caggtcgggt agcggcatag tatcgggaac aaggtaattt
     1561 tatgcggata gatggttttg gctcggggaa ttgccttcca tattttcgga tatggcggac
     1621 aaggagggga tgataatcct ttgttcaatc cagcctgacc atttcccaag caaaatattt
     1681 ttccaattat cggctttagg cataaattcg acaagggttt gggcgagggt gtcaaacgaa
     1741 agcgcggagg aacccattat ctccagcagc atcatatcga agccgtccaa tgtttggtac
     1801 atcacatcat cttcggcgtt tcgccatatt aacaaggctg tttccgcttc ctgcaaatca
     1861 tgggtaacat cgtatcgata ttgccggata aaggccgcag gggagggcgt gtatttgctg
     1921 tcatttgaat aatgaatgtc gggaatatcc ggaatttgtg caacttctgc cagcaattgg
     1981 gtatattcaa aatccatcag cgccaaaatg ccgtctgaaa gcggcgggct ttggcaatat
     2041 tgtaggaact cgccggggat ttcttgaaaa tagggcgttt gggcacgcgc gtcgcggacg
     2101 aaaccttctt tcagacggct ccattctttg ctgtcaaagt attgccgcgt ttcggtatag
     2161 cagcggtcga taaagctgtg gatattgttg cgtatcaggc ggatatagac gttcaatcgg
     2221 tcttgcggca gaccgtctgc ggcttcgccc ccgcgtatgg cttgggagaa acggtgctgg
     2281 tattgggcgg aggtttcagg ctgcacggcg gtattccttt ccggcacgcg tttgataatc
     2341 ggcaattttg gcaacttcgg cttcgagttc ggcaaaaggt gggaaattga aatcgcgttc
     2401 caacagggtg ggagggatgg tgggaagctt ggtgtaggtg agttcgagca agtcccaaac
     2461 ggtcggtaaa accgccgcgc cgtgggtatc aatcaaaagc tcgggggttt cggcatcgtg
     2521 tccggcgatg tggatgtagc acacgcgccc tgcgtccaca ttctctaaaa aggcctcagg
     2581 cgacagcagg ccgtgattga cggcgttgac gtaaatattg ttcacatcca aatgaatacc
     2641 gcagtcggct tcacgggcaa ccgcgttgag gaactcgacc tcgttcatct cggcaagcgg
     2701 agaatgcagg tagtaggacg tgttttccac ggcgatgcgg cagcccaaac ggtcttgtac
     2761 ttcgcggata cgccgcgccg tatgatgcac catttcttca gtgaaaggca gcggcaataa
     2821 atcgtaaaga tgaccgccgt cgtggcagta gctcaaatga tcggagaaaa acgtgcagtc
     2881 ataacggcac atcatttctt tgatgccgtc tatcaaatcg gtatccagcg gggcttgtcc
     2941 gcccagcgac atagataatc cgtgcaacgc cagcggcagc cgttccgcca cacggtcaaa
     3001 ctgtttgcgc gccctgccgc ccattttcag ccagttttcc ggtgcggctt cgataaagca
     3061 tatcgggctg ttttcggaaa gcgagagaaa gtcttccgcc aaatcgcggc ggtagcccaa
     3121 gcctgcgtgt tgaatcatat ttttacccgg ttcggatggc acaaaagaat gatgtgcttg
     3181 ttaaaaaaaa tctttgtttg ccgttaaaaa agccgcattt tactgaaaaa cgcggcttgg
     3241 tttgaagggg cgggattatt tagaaccgca tttgccttcg ccgcatttgc cttcggcaga
     3301 tttggctttg gctttagatg ctttggtgtg tttgtgggct ttttttacag ttgcaccgca
     3361 tttgccctcg ccgcatttgc cttcgcctgc tttagaagca gccgcgccgc acgaaccttc
     3421 ggcagatttg gatgcgccgc acgaaccttc ggcagatttg gatgcgccgc aagagccttg
     3481 ggcggatttt tgaacgcctg ttgcgttgct tgccggtttg tgggcggcaa cggcgccggc
     3541 ggccagagac agggataaag caccggcgag tgcggcagca atatttttgt tcatttctat
     3601 ttccttttca gaatatcaag ttaacgagat aaggttggaa tgtttaaagt cggattccac
     3661 gactgaatca atcttgtttc agacggcatt tggggcaaaa cagttttttc agcagcgtat
     3721 ccagcgacca tccgcccgca ccctggaaaa gcagcgggat taataccacg atataaatta
     3781 aagccatttt ataaccgttg tcgcagacat tgtaacccga accggcgtga accgcagccc
     3841 aagcgacggc ggtaacgacc atcagcccca atgccgacag gcgcgttgcc aaacccaaaa
     3901 gcaacagtac gggcagcaaa agctccgcat acatggcgag attccagttt aacgcgtccg
     3961 gcagcaagtt gaacgggaat ggaaactgat cgttgatttc ggaaaaccaa ttctccccgt
     4021 tccatttttg caaacccgat tccaaaaatt catatgcggc aaacagacgc aaaaccagca
     4081 tagcggatgc cgtctgccaa gttgttccga ttttcataag tgttcctgtt tttgttcctg
     4141 ttgatacccc gttagacgga ttttgtcgga aaaactgaca cccgtcagct aataatttaa
     4201 caagttaatg aatataaaaa gaaagtattt tgacggaaag aaagggaaaa aggcgggata
     4261 tttggataac ggcggtgttg tgagataatt cccgccttgg acggtatgcc ttcaaataca
     4321 atttccgtaa aggaaaaaag aaaatattaa ttttgatgat gacttaacaa agcccctttc
     4381 gcttggctgt caaaggagat tgttaagaaa agcaatctcc ctttgacggg gtgcgatatg
     4441 taaaggaatg gaaaatatgg taacgttttc aaaaatcaga tcatttttgg caatcgccgc
     4501 cgccgcgttg cttgccgcct gcggtacggc gggaaacaat gccgcccgca agccggtgca
     4561 aaccgccaaa cccgccgcag tggtcgcttt ggcactcggt ggcggcgcat ctaaaggatt
     4621 tgcccatata ggaattgtta aggttttgaa agaaaacggt attcctgtga aggtggttac
     4681 cggcacatcg gcaggttcga tagtcggcag ccttttggca tcgggtatgt cgcccgaccg
     4741 cctcgaattg gaagccgaga ttttaggtaa aaccgattta gtcgatttaa ccttgtccac
     4801 cagtggtttt atcaaaggcg aaaagctgca aaattacatc aaccgaaaag tcggcggcag
     4861 gcagattcag cagtttccca tcaaatttgc cgccgttgcc actgattttg aaaccggcaa
     4921 ggccgtcgct ttcaatcaag ggaatgccgg gcaggcggtt cgtgcttccg ccgccattcc
     4981 caatgtgttc cagccagtca tcatcggcag gcacaaatat gttgacggcg gtctgtcgca
     5041 gcccgtgccc gtcagtgccg ctcggcggca gggggcgaat ttcgtgattg ccgtcgatat
     5101 ttccgcacgt ccgagcaaaa atgtcggtca aggtttcttc tcttatctcg atcagacgct
     5161 gaacgtgatg agcgtttccg tgttgcaaaa cgagttgggg caggcggatg tggttatcaa
     5221 accgcaggtt ttggatttgg gtgcagtcgg cggattcgat cagaaaaagc gcgccatccg
     5281 gttgggcgag gaggcagcac gtgccgcatt gcctgaaatc aaacgcaaac tggcggcata
     5341 ccgttattga cgctatgccg tctgaacgta tttccagaag gcttcatcca tagaaaaaaa
     5401 tacccgaatc ccgtacaata cgcccctaaa attttgaaag aacgaaagaa tcatggaagc
     5461 cgaagtaatc aaccagctca acaacaccct gaatgatttg gagaagcgta gcgaagacat
     5521 ccgcgtttat atggattatc agggcaagaa agaccgattg gaagaagtca tcgggctttc
     5581 cgaagacccc gagctttgga acgacccgaa acgtgcccaa gaaatcggca aagagagcaa
     5641 aatccttgaa ggcatcgtgc tgacgctcga caacatcgct tcgggtatcg aagacaaccg
     5701 tatgctgatt gaaatggccg tcgaagaaaa cgacgaagaa ggttttgccg ccgtgaagga
     5761 agacgtggcg gggctggaaa aacagatggc ggatttggag ttcaaacgga tgttcaacca
     5821 gcccgccgac ccgaacaact gctttatcga catcaccgcc ggcgcgggcg gtaccgaagc
     5881 ggaagactgg gcggggatgt tgttccgtat gtacagccgc tatgccgagc gcaaaggctt
     5941 caaaatcgaa atccttgagg aagacgacgg cgaaatcgca ggcatcaacc gcgccaccat
     6001 ccgagtggaa ggcgaatacg cttatggttt gctgcgtacc gaaaccggcg ttcaccgctt
     6061 ggttcgctac tcgccgtttg attcgaacaa caaacgccat acctcgtttg cctccgtgtt
     6121 cgtttaccct gaaatcgacg attccatcga aatcgaaatc aaccccgccg atttgcgcat
     6181 cgacacctac cgtgcatcgg gcgcgggcgg tcagcacatc aacaaaaccg actccgccgt
     6241 gcgtattacc cacgagccga cggggattgt ggtgcaatgt caaaacgacc gttcgcaaca
     6301 cgccaacaaa gcggccgcga tggaaatgtt gaaatccaaa ctgtatgaat tggaaatgcg
     6361 caaacgcaac gaagagaaac aggcattgga agaaggtaaa tccgatgtgg gttggggcag
     6421 ccaaatccgt tcgtatgttt tggattcctc acgcatcaaa gacttgcgta caggctacga
     6481 agtcggcaac accaaagccg tattggacgg cgacttggac ggctttatcg aagccagcct
     6541 gaaacagggt gtgtagttta ggctgtatgc cgtctgaaac ctgtaccgtt tcagacggca
     6601 tttttgcgtt tcgtgttata atccgcgccg cacacgggac gggcagacag tcgccgcgta
     6661 tcgcgtaagg catacgggga ggaaagtccg ggctccgcag ggtagaatgc cggttaacgg
     6721 ccgggcgcgg taacgcgacg gaaagtggaa cagaaagcaa aaccgccgat ggctgctttg
     6781 gcagcacagg caagggtgaa aaggtgcggt aagggcgcac cgcgcatttg gtaacaatat
     6841 gcggcaggcc aaaccccatt cggagcaaga ccaaacagaa cgcaatgacg ctgcccgccg
     6901 agcgttcggg taggttgctt gagcataccg gcaacggtat gcctagagga atgactgtcc
     6961 gagacagaac ccggcttacc gcctgtccgg tgtgcatccg acacgcaaat gccgtctgaa
     7021 acctgaaata cggttcggac ggcattttta tttaggctgt gtttttatat tccgttgatg
     7081 cagccctgat gagtcggaca aaatgccttt tactcaatag gaccggtttc cgcccaagcg
     7141 gaaataaacg gctatttccc ttgcaaacag acggaatcag gcgggtattt aaatgacaat
     7201 cggcaggctg ccttccggtt gggggggggg aagcctgtcc tgtcgggtgg aaacggtttg
     7261 gtttcattat gcggaaaaat gccgtctgaa gggttcagac ggtatcggcg catcggggca
     7321 ggacttagtc tgtgccttcg agcattttgt tcaatttggg gacgagcagg agcagcagga
     7381 agcctgtggc tgcgccgatg tagaacagca gccggtagaa gccgatttcg tcgcccgcct
     7441 gatagccttt ttcaaacaat acgccgccca aagtgaagcc taatgaaaag gcaaggaaat
     7501 taagggcgac catttgggtt ttgaataaag gcggtgcgat tttggtggag atggacagcg
     7561 caatcgggga aatcatcagt tcgcctatcg tgatggcgag gacgatcagt gcgaaaaccg
     7621 caataggcat cggcgttccg gaggaaataa agggaacgaa tcccaaaaac gacgcgccgg
     7681 taacaaatac cgccatagcg aatttcagcg gggttttggg ttgtttgcgc cccatttttg
     7741 tccacattgc cgccatgagt ccggaaaaca ggatgaccca caggctttgc atagaatctt
     7801 tccaggcgac gggtacagta aacgaaccga tggtgcggtt gacggtttcg tcgaaataga
     7861 cggttgccac ggtgtaaatc tgaaaccaga cggcccaaaa catacagatg gtcaggaaaa
     7921 gcgggatgta ggcgatgatg tgccgtttgt tgtcggaact gacgcggggg ttggtcagca
     7981 ggcgggcgaa ataggcgatg acggcaagga tgacggtaga taagaggatg ccggagaaat
     8041 tgtcgaggtt gacaagcccg gttttgatgg cggttgcaag tgcggcgatg agggtgatgc
     8101 cgacggcggc cgcagttttg ccctgtcctt ttgaaagcgg atgggggacg gtggggtggg
     8161 gcaggttttt acgtcccaag gaataacgcc acaagccgaa tgccataccg accgccgccg
     8221 cgccgaaacc ataatggaaa ccgatgtttt cttgaagcag tccggtcaat aaggggccta
     8281 agaagccgcc gatattgatg gcgatgtaga aaatggaaaa tcccgcatcg cgcagcgggc
     8341 gcatttcgtc ctgttcgtat aatgcgccca ccatagaact ggccgtagat ttcacgccgc
     8401 cgctgcccaa tgcgatgaat atcagcccga ttagaaggcc gtacaggccc ggtgcggcgg
     8461 caaggacgat gtgcccgagc attacgacga tgcccgagag gaagagggtt ttttccgcac
     8521 cccatacgcg gtcggcaaac cacgcgccca aaatggtgga caggtacacg ctgccgctgt
     8581 atgcgccgac aatgccgcct gcgagggttt tgtctatgcc caagccgcct ttgtcggcgg
     8641 tgtagtagag gtaaatcagc aggatgccct gcattccgta aaatgaaaaa cgttcccaca
     8701 attcgatatg gaagagggtg gaaagctgga aggggtggcc gaaaaatgtt ttttctccgg
     8761 tgcgggcggg atgccgggac ataaaggttc tcctaaaaga ttttctctca ataccgtctg
     8821 aaacggatgc ggacggcggc ggtgtttttt tgtatgaaaa tatcggtttt taaccgatat
     8881 tttcattctt tgtcaaacga cgcgctgccg tttttcgcgg gcggatgttt ttatatttgt
     8941 ttcaattcaa tggattgtat tttagaggac gtgttccgat acggcgcggg taaatccttt
     9001 tctgtcaatg gcttatccga tagggcggtt tttacttaga tggaaaatca cttccttata
     9061 tatccgccac gccgaagggc gcattatggt ggattaactt taaaccggta cggcgttgcc
     9121 ccgccccggc tcaaagggaa cggttcccta aggcgcccaa gcaccgggcg aaccggttcc
     9181 gtaccatttg tactgcctgc ggcccgccgc cttgtcctga tttttgttaa tccgctatat
     9241 ttttcttaac catcccttcc aacagccgtg cgaaggtttt tttatatcag tgggaaatgc
     9301 aatattctat tgtttttatt gtagaatttt aaaaacagat tgttgtgttc cgcgtttttg
     9361 ccggtttgga agcggtgggg cgcatcagcc ttttataaag gcatatcgga agcctgtata
     9421 aggtttttga acatatcgat cctgttccct tgcaggcgga cggcagcggc ggatggcgaa
     9481 aggcgggttt tcaaatgtcc ggtttggcgg cgcgtctttc agacggcatg gcggcggagc
     9541 gtgccgcgcc gtctgaaaac cgtttatttc cctttgccgt ttccggcgga gtcttttttg
     9601 tccgaaccga agaggcggtc gaaacgtatg gtgtatgtca gctcgccgcc cgacgaacgg
     9661 ctgccgatac gggcaaccgc ctgtatggcg cgggtcagcc ggtaaatcag tttgacggac
     9721 tgttccgcgc tggagatgcc gtattcgtag ccgatgtaga gtttgccggt cagttgtttg
     9781 ccgacggtca gcacctgttc ggcggggttg agttcgccgg tttgcgcgtt gcggctgcgc
     9841 ttgctggtaa agcccaaatc atccaccagc ccgatgcggt cgttgatttg cccggcaagc
     9901 agcgcgcctg cggctgcgga cagggcggca ttgtcgccgc tgctgccgct gccggcacgg
     9961 ttgaggatga gccaggagag cttgtctttt tcactcatcg gttcgtttgc cgtcagcgta
    10021 atgcgcgggc tgttgaggct gcccaatatt tccacgcccg caccgacggg ggaaaggcgg
    10081 cgttcggcgc ggatgttgag gttggggtcg ttgagcgggc cgacaaagga gactgtgcct
    10141 ttggtaatgt ctaaatcctg cccgtatgct ttgtaacgcc ctttgatgac gcggaccgtg
    10201 cccaccccac gcacatttcc gcccggttgc gcggtcaggg tcagtttgcc gcctatggta
    10261 acgtccgcgc cgtagccgga gaagcggatg ccgtcattga ggtctaaagt caggttcata
    10321 ttgacgggga gcgatgccgc cgcctctttc ttgacttcgc ccaatacgac gacatcgtcg
    10381 ccgacggacg gcatcgagga tttttgcgaa ccgaacagcc cctgatcagt tttaatcata
    10441 ccggtaacgg atatgccttt ttgcggcgaa tagcgcaggc gggtgttgcc ggaaaccgtc
    10501 aggcggcggt tggggcggga caggatgcgg tatttgtcga acaccgcgcc gatatcgaca
    10561 tcgggcacgc tgttttccat gctgaccgtg ccggagagtt ccgccgtccc ttcgtgccgg
    10621 aatttcaggc tgtcgattac ccatttcctg cctgcaatat gcgaacgcag cgagccgttg
    10681 tccaagatga tgccttgggt ttggttgcgg taatagagct tgtcgccgtt gatactgccg
    10741 cccaagtgcg ggtcggcgat gctgccgccg agggttacgg cggcattcag gctgcctttg
    10801 acggtttgtc cgaccggtag gaagttgcgg aatacttcgg catcggcaac ggtcaggttg
    10861 agcctgccgc ccaaaggtgc ggtatcgaag gagcggcttt gcccgacggt gatattgccg
    10921 ttgattttcc cgtagttgct gctaccgttg acggcggcat tgacggacgg agagcttacc
    10981 cgtccgccga tttgcgcgga ggcattcagg ctgccggtaa tgttttgcgc ggcggcgggc
    11041 agaaagggct tcaatgcgcc caagtcggga agggaggctg taatcctgcc gccgagcggt
    11101 gtatttgcca tattgccgcc gaaggcgttg ccgatgccca aatcggcgtt aatccgtccg
    11161 aaacgcgcgc cgccgtcaag caggattccg atgcggtcgt tttgaaagcg cgttttcagg
    11221 gaaaatgcgt tcaaacccaa agcctgcccg ccgggcaata cggcatcgcc gctttgccgg
    11281 ctgatattga ggtagccgcg cgcgttgtgc ccgtaggcga catcccagtc gccgtttaaa
    11341 accagattgt gttcgaaggg cggtttgaag aaattgtgca actcggcgat gtgcaggccg
    11401 cgtgcgccgc cttttgccga tatgccggtt ttcctgtccc aagaaaagtg ttgcaggttg
    11461 aggctgccgc ccattgcctg ccaatttgcc gcacttgccg ccacgtgttc cgcaccggct
    11521 tcgagcgtca tacggttttg cagcttgagg ttgaatgcgc cgccgatgtc gaggatgccg
    11581 atgctgcctt tccatcgggt aagttccctg ttgatgccgc ctgaagcgtc caaatcgagt
    11641 ttgaacggtt tgccgtccag cgtcatggcg gcgtgtgtgc ggatgcggtg ctgcgcgccc
    11701 gtaccttcca gcgtcaggcc ggcggtatcg acaaccgccg cgccgcccga cagggaaagg
    11761 cggccgccct tgatatcggc gcgcatcggg cggcttgtgt cgggcgagcc tttgagggta
    11821 aaatcgagcg aacggatgtc tgccgctttg ccgatgtgta agttgcgcgc cgtgccggaa
    11881 aggtcggttt caaaggttcg gatgccgccg tccaaatcgc cggaaaggtg tccgcgtaca
    11941 tttaaagacc ccgcgagtcc gaaaccgaaa cgggataaat cgggtgcggt gatattgagg
    12001 ttaagccggt cgcctttttt gccgaagccg ccgtctgttt tgacgatgtt ccgccccaac
    12061 cgcaaatcga cggcggcgcg cggaaggtgg cgggactcgt aaacaatgtc ggcgctgccg
    12121 gcaatcggca cgccgttgaa cgtaccgggc aaaaaacgca ttttgcccgt aaatttctct
    12181 tttgccagtt caccggcaag atgaatcgaa ccgttgatat tgcctgccgg aaattgcgga
    12241 tcgatgcgcg aagggtcgaa tgcgcgggaa cggatgtcca gcttgagcag gcggtcttta
    12301 aacagctcga gatagccttg cgcggtcagg ctgccttccc cggcggagat gttgacggtg
    12361 tcgaacacca gtttccgctg ttcgtttgcg gggtcgcttg cgatggcgag gctgccgtcc
    12421 gtgcgtgccg tgccggtgcc gagttgccaa gagattttgg gcgaggcggt cgtgccgccg
    12481 atgccgatgc tgccgtccaa cctgcctttg aacgcggttt gcagcacgtc ttccgcgccg
    12541 acggagttga tgcctatatt taaatcaagg atgtcttttt cggtgtcgat tttgcccgac
    12601 agcctgatgc cgccccgtcc gagcagggcg gcggacgtat tgccgatatg caccgtgccg
    12661 tcctgccgga tgacaaagcc gcccaaaacc tgacggacgg ggatgccgtt gcggtcggca
    12721 aagccggctt tggtgttttc caaatcgagc gagccttcca gcgcgatgcc gtctgaaaac
    12781 gacgggatgg cggtcaggtc gaaattcagc ccggcatcgg gcagggaagg cacgaaggcg
    12841 gacggattga tgttgaatcc tttgaccagt acttcttcca atgttttatc caatgattcg
    12901 gcaaacgggt ggatgacgga ttttcccgag aggcggatat tgccgccgtc gatcgtcagt
    12961 tcggcgcgca catccttcag gctgccgctc agccgcgccg tactgtgtat ggtttcgcct
    13021 tcgaatccgc ctttggtgta aatggcggta tcgagggcaa acggtttttt caagccgacc
    13081 gaggctgacc ccgacgaact gctccacggc gtgtcggcgg ccttcaggtc gaggcgatgc
    13141 cctttacggt cgtaacggta tgccgcgttg aggcgttcga gatagacggt ttgtttgtca
    13201 aaggttttgc ccatgctgat tttgcccgtc tcgaagcggt cgagatagac ggcggcgggc
    13261 aggtctatgc tgtcgggcag gccttgaggc gggcgttctt ctttaggcgg agtcggtttg
    13321 gttacgatgg cgatgtcgcc ggcggagatg tcggtgatgt gcaggctgcg gcgcatcagt
    13381 tcggacggtt tccacgcgaa gcggaagcgg ctgattttaa ggtctgcccc ctcggtttct
    13441 atcgaccagt tgtcgccgtc gaagccgtcg agcagtgtgc ctttgaggtt ttgggaggaa
    13501 atgtttacgc cgaaccagga cgggatttgg tacagcccga agcgcaaacc tgcttccgta
    13561 ccggcgatcc agccgaggaa acatactgcc aaaatcagga cagacagcag tgccgccgac
    13621 agcttcagca gcgggcggcg tttttttgcc ggcggggcgg ggcggtgttc agacggcatt
    13681 ttgcgcgtgc cgttttcggt cggatcggta tctgtcggtg tggtatcggt cataatgtct
    13741 gtttcaaaat ggtttgctgc aatgccgtct gaagcggccg tatcggttta gaaacgcgtt
    13801 cccaagctga tgtgccagcg gatttttttg tcgctgtgtc cgtaggcgat gtcgaaggaa
    13861 aacggcgcga gcgggctgaa ccagcgcacg cccagtcccg aaccgtgttt cagcttcata
    13921 cgtttgaaat tggcggcggc atcgcccata tcgtggaata ccgcgccgga cagggtgcgc
    13981 gtaaacggca gttggtattc caagctgccc accaagaggg cgcattcggg caggaccgat
    14041 ccgttcggac cggcaagccc gatgctgtca agttcgtaac cgcgcacgga agacgcgccg
    14101 ccgctgcgga acatcagccc cgaggggaca tcggcattgt cgcgtgcaac ggtgtaaccc
    14161 gcttgtccgc gtatgatgaa cgtgccgagt tttttgtttt cgggcgtgaa gaaataacct
    14221 gcgcgggcag aggtgcggat tagcgcggtg gaggacagga atgtgcccaa agtcgtcccg
    14281 attttgccgt cgaggtaatg gccgttttcg gggtgcagca cgttgttgag cagctggcgt
    14341 ttccaagagg cggtcagcat cgtggcgtgg ctgttgccca aatcgacatc cgatccgggg
    14401 attttccggc cttctgcgag aaattccgcc cccagcctgg catcgatgcc cgcgcggtcg
    14461 cgcacatacc agatgccgcc ggagaaggcg cgtttttcga ggttttgggt ggtcgaacgg
    14521 ttgtaggaaa cgttgcttgt ccagtagttg ccccgatagt tgcgcggctg gctgatgccg
    14581 gcggcaagcg tggtttcgta tttgtccata tcccagacga ccgagccgat atagcctttg
    14641 ttgaagaggt tgtaatagtc gtaggcgatt ttgccgccca aaccgtattc cgaatcgagg
    14701 cggatgccgg tttcgagttt gtggcgtttg acctcggtta cgctgacttt gacggggacg
    14761 cggtcgcctt ggaggcggtc gaagtcggct tgtacggacg cgccggaata atgcccgttt
    14821 tgttcgagcg cctgttggaa gtcgagcagc aggtcgaggt cgtagggcgt gcccggttgg
    14881 aagcgcgcca ggccggagac ggtttgttcg gggtaacgct gtgtgccggt gatttcaaag
    14941 tcgccgaagg caatggggcg gccgctgtcc acgacgacgt tcaaatcggc ggtggcggta
    15001 tcggggttga cggccgcccg ggtgttgccg agcttggcaa gcgggtagcc tttgcgcgtt
    15061 accgcgccga ggacggaagt tttgctgttt tcccaactgt cctgatcgaa atcgctgcct
    15121 accggctgct gccagttttc cagcgcgttg cggtagtatt cggcgaggtt gccgtctgaa
    15181 aggatgtcgc cgaggatggc gacgccgacg ttggcgattt tggtgcgcgg gcccggtgtg
    15241 atgtgcaccg tataagctcc gtctttttcc gtcaggctga ccttgctgct gaaatagcct
    15301 ttgctgcgga gcatggtttt gacgttgtcc ggtgcttctt cggcaaggaa tcccgtctgt
    15361 tccttatcca aaacctcttc ctgctgctgc gtgatgagcg gcaggtgttc ttcgaccata
    15421 tctttgattt cactgtcctg cgtgtcgatg cggacgggga atttgggttt taatttgact
    15481 gattcggtgt cggggctttt gcttttgaac aatgcgaaac ccgccgcctt gttttcggaa
    15541 aggtcggcgg caggcgcgta tgcgtgcgga aagaaaaata aagccggcag gagcagggag
    15601 gtcggtttga tcatcatggt acgggtgtcg tgcatcggtg tggcggattt cgattctaac
    15661 actgttcgga cgggacggga cgcttcagac ggcataccgc cgccggtatt tcggaggttg
    15721 cggcccgccg gaaatgtatc tgcttgtttt aaggcatttt gtttccaatc cgcatatgga
    15781 aaacaggctg cgtttcggaa tagtgtcgaa aaataggcag gattaaataa atttattctc
    15841 gttcaataaa ttaaaccgtt gcgccgaatg ataaaacaaa gcctgtccct gcatagggca
    15901 aatcaacgca cctcaaaaca cagcttgaaa aataaaaaaa cacagagaat gggttttctt
    15961 ttaaaataca acatttttta acatatcaca acataaggaa gaattatggc gttcggcaaa
    16021 tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg
    16081 ttgggcatcg taatcggttc ggtttcaccg caactgggct tggcggcagg cttgttcggc
    16141 agcctgtttg tcggtgcgct caaagcggtc gcgccggttt tggtatttat tttggtggcg
    16201 gccacaatcg cgcagcacca aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt
    16261 tacctcatcg gcacgttttc cgcagccctg accgccgtca tcgccggtat ggttttcccg
    16321 acgcacattg ttttggcggg cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg
    16381 gaagtgttga aatcgctgct gatgaacctg gtcgccaacc cgattaacgc gattgccaat
    16441 gccaactata tcggcatttt ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac
    16501 ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg
    16561 aaatggatta tccgttttgc ccctttgggt attttcgggc tggtgtcgtc cacaatcgcg
    16621 gagacgggtt tcggcgcgct ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt
    16681 atggcgttta tcgcgctggc ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac
    16741 ccttatccgc tggtgtttac ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt
    16801 tcttccgccg ccaatatccc cgtgaatatg gctttggcga aaaagctggg gctgcacgaa
    16861 gacacttatt cgatttccat cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt
    16921 acgattaccg ttttggctat ggcggcggcg cacacccaag gcatacaggt tgattttgcc
    16981 accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcc
    17041 ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt tcggcatcag caacgatgtc
    17101 gccatgcagg tggtcgccgt cggcttcatc atcggcgtga ttcaggattc ggcggaaacg
    17161 gcgttgaatt cttcaaccga cgttttgttt accgctgccg ccgatttggg ccgacagaga
    17221 aaccgtgcgg aataaaccaa gctatgccgt ctgaagcccg tttggcgttc agacggcata
    17281 tttttacaat tttatgggca tatggcggaa acaataccgg ctgccttgaa tatgtttcaa
    17341 cacacagggc gatacataaa gcgtcgccct atgaaggaga tgatgtttaa acgcagtgtg
    17401 attgcaatgg cttgtatttt tcccctttca gcctgtgggg gcggcggtgg cggatcgccc
    17461 gatgtcaagt cggcggacac gccgtcaaaa ccggccgccc ccgttgttgc tgaaaatgcc
    17521 ggggaagggg gtgctgccga aagaaaagaa agatgaggag gcagcgggcg gtgcgccgca
    17581 aaccgatacg caggacgcaa ccgccggaga aggcagccaa gatatggcgg cagtttcggc
    17641 agaaaataca ggcaatggcg gtgcggcaac aacggacaac cccaaaaatg aagacgcggg
    17701 ggcgcaaaat gatatgccgc aaaatgccgc cgaatccgca aatcaaacag ggaacaacca
    17761 acccgccggt tcttcagatt ccgcccccgc gtcaaaccct gcccctgcga atggcggtag
    17821 cgattttgga aggacgaacg tgggcaattc tgttgtgatt gacggaccgt cgcaaaatat
    17881 aacgttgacc cactgtaaag gcgatccttg taatggtgat aatttattgg atgaagaagc
    17941 accgccaaaa tcagaatttg aatcgttaag tgatgaagag aaaattaaga aatataaaaa
    18001 agatggagaa aagtttactg gtttggttgc tataaaagtt gaaaataatg ggttgaataa
    18061 atataccatt atttatcaag ctcagcctac tcgttctgca cggtcgagga ggtcgcttcc
    18121 ggccgagatt ccgctgattc ccgtcaatca ggccgatacg ctgattgtgg atggggaagc
    18181 ggtcagcctg acggggcatt ccggcaatat cttcgcgccc gaagggaatt accggtatct
    18241 gacttacggg gcggaaaaat tgcccggcgg atcgtatgcc ctccgtgtgc aaggcgaacc
    18301 ggcaaaaggc gaaatgcttg ttggcacggc cgtgtacaac ggcgaagtgc tgcatttcca
    18361 tatggaaaac ggccgtccgt acccgtccgg aggcaagttt gccgcaaaag tcgatttcgg
    18421 cagcaaatct gtggacggca ttatcgacag cggcgatgat ttgcatatgg gtacgcaaaa
    18481 attcaaagcc gccatcgatg gaaacggctt taaggggact tggacggaaa atggcggcgg
    18541 ggatgtttcc ggaaggtttt acggcccggc cggcgaggaa gtggcgggaa aatacagcta
    18601 tcgcccgaca gatgcggaaa agggcggatt cggcgtgttt gccggcaaaa aagatcggga
    18661 ttgatgttga tgccgcccga agcgcaggta ttcattacgg cgggacgacg cgttcaaatc
    18721 atcaaacaag cccccgaaaa aggcggcatc ccaaaatccg gcactgaaat tgccgcgcgt
    18781 atcggcggca ttccgcagcc tgccggggaa accgtcggca gcagccggaa tgtcgggtat
    18841 ttccgcccgc ctttttcggg ctgttgaaat atgaaaataa agcataggcc gtctgaatgt
    18901 tcagacggcc tgttttttta acggctgtgt ttcaacactc cgccggttcc aaccggcagc
    18961 agcagggaga taatgccttg aaccccgctc ttcggggcgg cagggttcgg gccggtttgg
    19021 ggaaaggcat cgcctttccg aatcggggcg gtgcaacggg gtgcgcctta tgtgccgaaa
    19081 gggtttgcgg caacggtatg ccgtctgaag cgggtgcgtt cagacggcat tgtgatgttt
    19141 ggggcgcggg tccggttcaa atccgcatca gttgataaac ccgttgtctt tcattttctt
    19201 ttcagaaaaa tactggccgc ataccagcag ggcggcgagg atgatgacaa acagccctgc
    19261 catagtcagt gtgattttga agaacaattc ggcttcgaga aaagaaaacc aaagttgcac
    19321 aatcagcagc agcacccagc cggcaaacag tccgagacag gcataaacgc cgagcttaaa
    19381 cacggtcccg ctccaaaatg acgacgtagg cttcgcgttt gaaaaacagc agcatacggc
    19441 gcaaatcttt ttccatcgtt acgacccgcc agccttcggg ggtttgtttg ttgaggaacg
    19501 cgctgaaatt gacggggttg acctttgccg cgccgaaaaa caggctggac aacaggcttt
    19561 cctgataaat gacgactttg tattctttca tcgtatttcc ttttcggttg aaaccccgcc
    19621 ctttggggcg gtaggatcag actttatttg ggagggaacc ccttccaaat caggacgata
    19681 cataggtcgg tgctttatgt atcgtcctgt gtgttgaaac ataggattcc tttaatcaag
    19741 ggattgcctt ttcaggcaat cccttaaatt gcagcgatgc aatgcggcga aatttattgc
    19801 ttattgccca actgcggcaa taccgagcct tcgcccagcg agagcaaacc gctttgcgaa
    19861 taaataccca gtttggcgcg ggtgtcggtg atgtcgaggt tgcgcatcgt gagctgtccg
    19921 atgcggtcga gcggtgtgaa cgccgcacct tcgacttttt ccatactcag gcgttcgggt
    19981 tggtaggtca ggttgggcga ttcggtgttc agaatcgagt agtcgttgcc gcgccgcagt
    20041 tcgagggtaa cttcgccggt aacggctttg gcgacccagc gttgggcggt ttcgcgcaac
    20101 atcaaggctt ggctgtcgaa ccagcggcct tggtagagca aacgtccgag gcgcaggccg
    20161 ttgatgcggt attgttcgat ggtgtcttcg ttgtggatgc cggtaaccaa gcgttcgtag
    20221 gcgatgtgga acaatgccat gcccggggct tcgtagatgc cgcgcgattt ggcttcgatg
    20281 atgcggtttt cgatttggtc gctcataccc aagccgtggc ggccgccgat gcggttggct
    20341 tcgaggaaga gttcgacggg gtcggcgtat tctttgccgt tcaatgcaac cggcacgcct
    20401 tcttcaaagc gcacgctgac ttcttcgggt tcaatttcga cgttttcgtc ccagaaggca
    20461 acgcccatga tgggcttaac gattttgatg cccgagttca aaaattccaa gtctttggct
    20521 tcgtgggtcg cgccgagcat attggaatcg gtggagtagg ctttttcaac cgacattttg
    20581 tagttgaagc cgttggcaat cagaaattcg ctcatttcgt gacggccgcc gagttcgtcg
    20641 ataaattgct ggtcgagcca gggtttgtag attttcagcg cgggattggt gagcaagccg
    20701 tagcggtaga aacgctcgat gtcgttgcct ttgtaggtgc tgccgtcgcc ccagatattc
    20761 acatcgtctt ctttcatggc ggaaacgagc atggtgccgg tcacggcgcg gcccagaggc
    20821 gtggtgttga aataggcgat gccgccggtg gaaacgtgga acgcgccgca ttggatggcg
    20881 gcgatgcctt cgtgtgccag ttgtgcgcgg cagtcgatca ggcgggcgtt ttccgcaccg
    20941 tattccattg cttttttggg aatggcgttg taatcgtctt cgtcgggctg gccaaggttg
    21001 gcagtgtagg cataaggcag cgcgcctttg agtttcatcc acaacagcgc ggcagaggta
    21061 tcaagaccgc cggagaaggc gatgccgact ttctgaccga cggggaggtt ttgtaaaata
    21121 gtatggtttt ggttcatgaa aaaatcctcg atagcttgaa aaatcaaagc cattatctta
    21181 taaatagaaa atattgtcag ccatcatgcc gtctgaacag gtattccgcc gtttcaggcg
    21241 gtatttaaaa ccgaaaacat tatgaacgcg ttcaacctca tcatcatcgg agacgaaatc
    21301 ttacacggca gccgccaaga caaacatttc gcctttttta aatccctgct cgaatccaaa
    21361 gggctgaagc tcaatcaggt gcaatatctg cccgacgaac ccgatttgct ggtcaggcaa
    21421 ctgcgccgca gcttttcaga cggcataccg acgttcgtta ccggcggcat cggcgccacg
    21481 cccgacgacc gcacgcgcca aaccgcagcg gctgctttgg atttgcccgt cgtccgccat
    21541 cctgaagccg ccaagtttat cgaaggcata acccggaaac gcggcaagcc gctcgattcg
    21601 cccgaacacg cccaacgcct gaaaatggcg gatttccccg agggcgcaga gcttgtgccc
    21661 aacccgttca acaacatcgc cggattttcc atccgcgagc attatttctt ccccggcttt
    21721 cctgtgatgg cgcacccgat ggcggaatgg gttttggaaa cttattacgc cggccgcttc
    21781 aaccaaaccg aacgcggcag ccgcagcgtg tatgtgtttg agcagcccga atcgcgcatt
    21841 acgccgatta tagagcatat cgagcaaact tatcccggcg tgcgttcgta cagcctgccc
    21901 agcgtcggtt ggacgcattc agacggcacg caggtcaaac cgcacattga gttcggcatc
    21961 aaagccgaag gagaagccgt caaccttttg gacgcggcgt gggcggaagt gttgcacagc
    22021 ttggacggat tgggagcaaa gctgaaaaat cgggtaaact gaaaactgtt tgcccaaatc
    22081 ggacgatatt taaggaaaac catgcaatac cgaatcagaa gagaaaacga agcacccgaa
    22141 gcaaaaaatg cgggggaaac cttatgggag cgcgacatta tgcgcgaagt gctgctgtcc
    22201 gcctatcggg acaggcgcag ggagcggatg tggaaaaaca tctggcgcgc cgtcagcacc
    22261 ctgattctgg ttgccctgat tgcaggcatt ttccgaaaag acgaagccgc attgcagttg
    22321 gcgggcaaca cgccgcatac cgcagtcgtc aacctgtacg gcaaaatcgg caacggcgta
    22381 gaagaccagg tcaaaaaact caaagacggg atggaggccg cctacaaaaa cccgcaggca
    22441 aaagccatcg tcatccgcgc caacagcccc ggcggttcgc ccgtcgtctc caacaccgct
    22501 tttgaagaaa tacgccgtct gaaggcgcag caccccggca tccccgttta tctcgtggca
    22561 gaggatatgt gcgcgtccgg ctgctactac atcgcggcgg cggcggacaa aatctatgcc
    22621 gacccgtcca gcatcgtcgg cagcatcggc gttatcggca gcagtttcga tgcgaccggc
    22681 ctgatggaaa aaatcggcgt aaaacgcagg gttaaaattg cgggcagcaa caaaggcatg
    22741 ggcgatccgt tttcgcccga aacgcccgaa cagtcgaaaa tctgggaaga aatgctgacc
    22801 ggcatacacg gcgaatttat caaagccgtc aaaaccggac gcggcggccg cctcaaattc
    22861 cgacaatatc ccgacgtgtt cagcggtcgc gtgtacacag gcgcggacgc gctgaaagtc
    22921 gggctggtgg acggactcgg caatatttac agcgtggcgc gagacgtggt caaagctccc
    22981 gatgtggtgg attacactcc gaaggacgat ttcggcagaa tcttgggcag acgcttcgga
    23041 gcagaactca aagcaagcgt ttcggaagct ctgcaggccg tccggtagcg ttgtttacag
    23101 tatttctaag ggaataccag aataaaaata tacacaaaaa cactcacctc cccagtatgc
    23161 tgccactaag tcagccgcgt tactatttac cgtgcactta aagccgcaaa aggcaggttg
    23221 tccccgggag gttttgtgca tcctgatacc gatcggttgc tgttgctgtt gctgttgctc
    23281 aaaggacgga aggccgccga taaacgagat tacctgtttg tcgctattga cgatttttat
    23341 actctgccat tttgccggac aaaaccgcag acagtgctgc caagtttctg accgaacatc
    23401 tggctgaccc ctgcttgtac ctggttgagt acgtttactc tgacaatgat aggtaatata
    23461 aagagccgtc caacatgctt tcggtgcagc ttgttatgat aatgggattg gttggaggct
    23521 tgcccgattt gcttgtccgc agaccaacgg taaggcggag cgggttatcc gtaccttgat
    23581 ggagatgtgg catgaggaac agtcgtttga cagtccggaa catcagcaaa aggagttgtg
    23641 ctgctttatt aatttaggcg ctgtcctaga taactaggga aattcaaatt aagttagaat
    23701 tatccctatg agaaaagccg tctaagccgg tataaacaaa ataaactcat tgggctattt
    23761 gtcgcaggtg taactgcaag aacagcggca gagttggtag gcgttaataa aaataccgca
    23821 gcctatgatt ttcatcgttt acgatgactg atttatcaaa acggtccgca tttagaaatg
    23881 tttgatggcg aagtagaagc agatgaaagt tattttggcg gacaacgcaa aggtaaatgc
    23941 ggtcgcggtg ctgccggaaa agtcgcagta ttca
//
LOCUS       DAXFHF010000029        23461 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXFHF010000029 DAXFHF010000000
VERSION     DAXFHF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23461
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.029"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(197..754)
                     /locus_tag="ACFIRM_001073"
     CDS             complement(197..754)
                     /locus_tag="ACFIRM_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3028719.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(829..2463)
                     /locus_tag="ACFIRM_001074"
     CDS             complement(829..2463)
                     /locus_tag="ACFIRM_001074"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC3028720.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2526..2867)
                     /locus_tag="ACFIRM_001075"
     CDS             complement(2526..2867)
                     /locus_tag="ACFIRM_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028721.1"
                     /translation="MIFKCRPNVFQTASAVSAFSGGGWGCGVKLRCRRLSVLFNPGVM
                     LFVPDCPVGGASGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLV
                     FLPCFAPLPYN"
     gene            complement(2864..3970)
                     /gene="serC"
                     /locus_tag="ACFIRM_001076"
     CDS             complement(2864..3970)
                     /gene="serC"
                     /locus_tag="ACFIRM_001076"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC3028722.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4336..4767
                     /gene="rimP"
                     /locus_tag="ACFIRM_001077"
     CDS             4336..4767
                     /gene="rimP"
                     /locus_tag="ACFIRM_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC3028723.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4795..6297
                     /gene="nusA"
                     /locus_tag="ACFIRM_001078"
     CDS             4795..6297
                     /gene="nusA"
                     /locus_tag="ACFIRM_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC3028724.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQIILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6309..9140
                     /gene="infB"
                     /locus_tag="ACFIRM_001079"
     CDS             6309..9140
                     /gene="infB"
                     /locus_tag="ACFIRM_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC3028725.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9300..10640)
                     /locus_tag="ACFIRM_001080"
     CDS             complement(9300..10640)
                     /locus_tag="ACFIRM_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC3028726.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10714..12054)
                     /locus_tag="ACFIRM_001081"
     CDS             complement(10714..12054)
                     /locus_tag="ACFIRM_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC3028727.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVVASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12197..12823
                     /locus_tag="ACFIRM_001082"
     CDS             12197..12823
                     /locus_tag="ACFIRM_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC3028728.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12888..14306)
                     /locus_tag="ACFIRM_001083"
     CDS             complement(12888..14306)
                     /locus_tag="ACFIRM_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3028729.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     MQKKAGQDLSYQYEFHEFHDKQ"
     gene            14633..15361
                     /locus_tag="ACFIRM_001084"
     CDS             14633..15361
                     /locus_tag="ACFIRM_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC3028730.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15420..15908)
                     /locus_tag="ACFIRM_001085"
     CDS             complement(15420..15908)
                     /locus_tag="ACFIRM_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC3028731.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     ILATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGAAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16727..17191)
                     /locus_tag="ACFIRM_001086"
     CDS             complement(16727..17191)
                     /locus_tag="ACFIRM_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC3028732.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHVHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17413..18471
                     /gene="alr"
                     /locus_tag="ACFIRM_001087"
     CDS             17413..18471
                     /gene="alr"
                     /locus_tag="ACFIRM_001087"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC3028733.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(19013..20368)
                     /locus_tag="ACFIRM_001088"
     CDS             complement(19013..20368)
                     /locus_tag="ACFIRM_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC3028734.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(20383..20655)
                     /locus_tag="ACFIRM_001089"
     CDS             complement(20383..20655)
                     /locus_tag="ACFIRM_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC3028735.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(20780..21516)
                     /locus_tag="ACFIRM_001090"
                     /pseudo
     CDS             complement(20780..21516)
                     /locus_tag="ACFIRM_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
     gene            complement(21654..22553)
                     /gene="prmB"
                     /locus_tag="ACFIRM_001091"
     CDS             complement(21654..22553)
                     /gene="prmB"
                     /locus_tag="ACFIRM_001091"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3028736.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            complement(22652..22849)
                     /locus_tag="ACFIRM_001092"
     CDS             complement(22652..22849)
                     /locus_tag="ACFIRM_001092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028737.1"
                     /translation="MGERLVQTDYAHPAVGIADIGHLVVFCGYPLLALNKPRFFPAYQ
                     VAHTVRHRRFRSGNGLRAAFM"
     gene            22885..23370
                     /locus_tag="ACFIRM_001093"
     CDS             22885..23370
                     /locus_tag="ACFIRM_001093"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028738.1"
                     /translation="MRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAET
                     KELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVL
                     MNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKD
                     K"
BASE COUNT         6191 a         6415 c         5804 g         5051 t
ORIGIN      
        1 ccgattccga tgccgtctga accttcagac ggcatgaggt ctttgcctgc gcacttggtg
       61 cgctggtcgc ctccgaacat ggcgcaacac ccgacatttc cgccgaacgc atcgggcgtt
      121 tcataaaccc ggtttaaaac gcatggaaaa atgccgtctg aaagcctttc agacggcatc
      181 ttgtttgaga ttccgtttac caatggctga caaacgcttc caaatcggta ttcttgggct
      241 tatgcacttc ctctgtcggc gtgccgacca tcatcagccc gatgatttta tccttatcct
      301 cgcaaccgaa agcctcccgc aacaatgggc tgttgaccca catccccgta atccagacat
      361 tgtcgaatcc ctgagccgtt gccgccagtt gcagcgcata cgccgcacaa cccgccgtca
      421 gcatctgctc ccattccggt ttcggcttag gcacatcgcg gttcggcaca aacgttaccc
      481 cgatgaccat cggcgccata ttgcccactt tttccgcctt tttcatcgca tcgtcgccga
      541 aattcagttc ggcaaccgtt tgcttcaaca catcgcgaaa acgttgcaat cctacctcgc
      601 cttgaatcac ggtaaaacgg aaggggcgca tgttgccgtg atcggaaact tgggttgccg
      661 cctgcaatat ttgttccaac tccgccgcat cgggggcggg gtgcttcagc tttttggaag
      721 atcggcggtt cgtcaataat tttaaagcat ccatatcgtt atactccggt catcggtcgg
      781 gtgtttcgga cacatatgcc gtgaaggctt cagacggcat atccggcatc agcgcggacg
      841 gcggcaggct gccaatatat ccatttcctt ccgataggtt tggctgttgg aaatgtccat
      901 cagccccaat accgtgctga aatagtggtc gtgcgaatat tcgttttccg ccgctttttg
      961 tttgaggcat tggaaatcta tgcccccgtg ttggcggaag gctttggaaa accacataac
     1021 catcgggata tgcgtctgtc cggaaggcgc gatggcgtaa ggcgcggcgt gcaggtacat
     1081 cccgttttcg cccaaacttt cgccgtggtc ggaaacataa tacaccgcgc tttccaaatc
     1141 gtcgcggttt tcaagtttgc ggataacctt gtcgataaac tgatccacat acaaaaccgt
     1201 attgtcgtaa gtgttgacca acgtggcgcg ggcgcatttg tcgatttcgt tggtatcgca
     1261 tgtcggcgtg aatttgcgct cggcttcggt atagcgttcg taatacgtcg gcccgtggct
     1321 gccgatggta tgcaggatta aaaccgcgtc tttatcgttt ttgttgaggg cttcgtcgaa
     1381 cttggtcaac aggatattgt cgaggcactc gccgttgcgg cagtattcgg gcaggttgag
     1441 cgaggtaacg tcggtattcg gcactttgcc gcacacgccc ttgcagccgg aatcgttttc
     1501 caaccaagta acttccacgc cggcgcgctg tacgatgtcc agcaggttgt cttggtgttc
     1561 ggctttgatt tcgtcataat ccgtgcggtc gaaggttgag aacatacacg gcagggagtg
     1621 cgcggtcgat gtgccgcagc ttctgacctg cgggaaattg acaatttcat cgccgcgcgc
     1681 ggcaagcagc ggcgtagttt ggcgactgta accgttcaaa ccccagttgg cggcacgcgt
     1741 ggtctcgccc acgaccagca ccacgaaacg gcgcaggctg ccggccggcc ggttttgcac
     1801 gaccgccata tccaattgcg tataaggaat attggaacgc ttccaatctt tgtatttcga
     1861 cacgcccgcg ccgatgaaat tagacggcac aatcagatgg gttactgatt tattgttgcg
     1921 gaaaaacgag gcgtaatcct gatattgcaa catcgcgatg cccaacgcgc acaaaaagga
     1981 aacggcggca agcacaaggc gcgtcaaaag ctccttatac caaacgcggt atttgacctt
     2041 gacggcgata tatgccagcg caggcaatac gcccaaacat acaatccaca gcacatagcc
     2101 cggcgtaatc aggcgcgcgc tttccgccgc cgtggtttgc aagacattat tcaacatcga
     2161 cttgttgaaa tagatattga aaaatatttc ttggtaagac accgccgcac tgataaccaa
     2221 tatcaatgga atcaacactt tatgcacaaa aggcagggca atgacgtgaa aaacgaaatt
     2281 acttaaaaaa aacagcacca ccggcatcgt atagaggaag atgtccgccc cggtgtcgtt
     2341 aaaagggcga agttcgacaa ctttggcaaa aaaggcgtaa ttcaatacca gcgaagaata
     2401 cagggaaaga aaggcaatca gcgcggaaga gccgagcttc ggcctcaggt tcggttttat
     2461 catttggaat gtgtcggata agggttggaa aaggcatccg gaatccggat tattgaaaaa
     2521 gattcttaat tataaggcaa cggagcaaag cagggcaaga aaacgagtag gcggcgcggg
     2581 ggtttccgcc cgccattcaa acgtccggca gacataaaaa catcgtaagc aaaattcgaa
     2641 ccggtctgca accgcccctg ccggaaaagc gggcaaagca tttcatattg gaaaaacccg
     2701 gacgcgccgc cgacgggaca gtccggcaca aacagcatca cgcccggatt gaaaaggacg
     2761 gatagccgcc ggcagcgcag ttttaccccg catccccaac cgccgccgga gaacgccgaa
     2821 acagccgatg ccgtctgaaa cacattcggg cggcatttga aaatcaaccg taacgccgtt
     2881 ggaaatcgcg cataaaatct gccaaagccc gcacgccttc aagcggcatc gcattataaa
     2941 tgctggcgcg catcccgccg acagacttat agcccttcaa caggcacaag ccctgcaact
     3001 cggcttccaa cacaaaacgg cggtcaagct cctcatcccc cgtttggaac acgacattca
     3061 ttttagaacg cgcattcgga cggatacggt tgatatagaa accgccgctg ccatctatcg
     3121 tctcatacaa ggtttgcgcc ttcagccgat tgaccgcttc aatttttttc acaccgccct
     3181 gcgcctgcag ccagcggaac accagccccg acatataaat cgcgtaagtt gacggcgtgt
     3241 tgtacatacc gtcgcggttc aaatgagaac ggtagttgaa cacatcggga atatcgttcg
     3301 gacaacgctc gagcaaatcc tcacgcacaa tcaccaccgt aactcctgcc ggcccgatgt
     3361 ttttctgtgc gcctgcgtaa atcagtccgt agtcggcaac atcaaactcg cgcgacagaa
     3421 tctcgctgga catatcgcac accaacggcg gcatgccgtc tgaaaggcgc ggcacttcac
     3481 ggtattgcag cccgttgaca gtttcattga cggcaaaatg gacaaacgcc gaatcgggtg
     3541 caacatccca cgtttccaca ggcggcaggt cgagatagtc gaactgctcg cccccgtgcg
     3601 ccgccaaacg gatttccgta tcggtcaaac ggctcatctg ttcataggcg atacggctcc
     3661 agttgcccgt taccaccgcg tcggcagtgc ggaaaccgtg tgccagattc atggctgcca
     3721 tattaaattg ggttgttgct ccgccctgaa gaaacaatat cttatagttg tcaggcactt
     3781 tcaaaagctg cctcaaatcc tgttccgcat gatgcaggat gcttaaaaac atttccgaac
     3841 ggtggctcat ttccatcacg gaaaaacccg taccgttgta gtccgacatt tcctgctgcg
     3901 ccgtcctcaa cacagcttca ggcaacacgg cagggccggc ggaaaaattg taaatcggat
     3961 aaagagacat gatgcagccc tggttctgaa caataacccg cccgatttta ggcttgcagc
     4021 aggctttgtg caaggatgga aaatacctgc cctccgcccg attccatgcc gcccgaacac
     4081 gggaaataat atcaatatat tgatttacaa acataaaaat catgctagcg acaaatagat
     4141 acatttgttt tgtcaacaat attcacgatt tcccattaca aacctcccgt acacccgctt
     4201 ttttccgtcc caaaaacaca aaataaatca acactttcat ttctccgcaa aagcggttat
     4261 aatcacgccg atttttcaac ttttgacgaa aaatggccac ggggccattt ttttattatg
     4321 tacataggga gcagcatgga tattcaaacc atcctcgaaa aaaccctgcc cggcctggga
     4381 tacgaactgg tcgatttcga actgaccgcg caaggaacat tgcgcgtgtt catcgacaaa
     4441 gaaggcggca ttaccgtcga agactgcgcc accgtcagca accacttgag ccgcgtcttc
     4501 atggttgaag acatcggcta caaaaacctg gaaatttcca gccccggact cgaccgcccc
     4561 ttgaaaaaag ccgccgactt cgtgcgcttt gccggtcaga atgccaaaat caaaacccgc
     4621 ctgccgatag gcggtcagaa aaactttatc ggtaaaatcg aaggctgcga aaacgacact
     4681 gttaccgttt ccttcgacgg caaaaccgtg caaatcgaat tgggcaacat cgacaaagcc
     4741 cgcctgcgcc ccgaattcaa attctaaaac acagcaatat tggagatgtt caaaatgagt
     4801 cgtgaaatgt tacagctggc agaagcactg gcaagcgaaa aaaacgttga tgcggaagtc
     4861 gtcttccaag cactggaatt cgcactctct accgccgcca agaaaaaggc agaccgcgaa
     4921 cacatggacg tgcgcgtcca aatcaaccgc gataccggcg aataccaaac cttccgccgc
     4981 tggctgattg tggccgatga agactatacc tatcccgatg tcgaaaaaac catcgaggaa
     5041 atccaagagg aaatccccga tacgaccatc caaatcggcg aatactacga agagcagctg
     5101 cccaacgaag gcttcggccg ccaagcggcg caaactgcca aacaaatcat cctgcaacgc
     5161 atccgcgatg ccgagcgcga gcagaatctg aacgagttcc tcgccgtcaa agaagacatc
     5221 gtgtccggca cggtcaaacg cgtcgaacgc cacggcatca tcgtcgaagt cgttgccggc
     5281 aaactggacg cgctgattcc gcgcgaccaa atgattccgc gcgaaaactt ccgcagcggc
     5341 gaccgcatcc gcgccctctt cctgcgcgtc gaagaaatcg gcaacaccgg ccgcaaacaa
     5401 atcatcctga gccgtacttc cggcgatttc ctcgtcaaac tgtacgccaa tgaagtacct
     5461 gaaattgcag gcggcatgct tgaaatccgc gctgtcgccc gcgacccggg ccaacgtgcc
     5521 aaagtcgccg tcaaagccaa cgaccagcgc atcgatccgc aaggcacctg tatcggcgtt
     5581 cgcggttcgc gtgtcaatgc cgtcagcaat gaattgtccg gcgagcgcat cgacgttgtc
     5641 ctttggtcgc ccgagcctgc ccaattcgtc atgagcgcgc tctcacccgc cgaagtcagc
     5701 cgcatcgtca tcgacgaaga caaacacgcc gtcgatgtca tcgttgccga ggaccggctc
     5761 gcgctcgcca tcgggcgcgg cggtcaaaac gtgcgccttg cttccgacct gaccggctgg
     5821 cagctcaaca tcatgacttc cgccgaagca gacgaacgca atgcggcaga agatgccgcc
     5881 atccgccgcc tgtttatgaa tcacttgaat gtagatgaag aaaccgccga cgtactggtt
     5941 caagaaggtt ttgcaacctt ggaagaagtc gcctatgttc ctgccgccga actgcttgcc
     6001 attgaaggat ttgacgaaga aatcgtcgat atgctccgca accgtgcccg cgatgccatc
     6061 ctgaccatgg cgattgctgc cgaagaaaaa ctgggcgaag tgtccgacga tatgcgtaac
     6121 ctcgaaggcg tagatgccga tatgctcctc agccttgccg aagcaggcat taccacccgc
     6181 gacgacttgg cagagcttgc cgtggacgaa ctgattgaaa tcaccggtgt aaacgaagaa
     6241 actgcaaaag ccgtcatcct gaccgcacgc gaacactggt ttaccgaaga caaataaagg
     6301 gggtacagat gagtaacaca accgtagaac aatttgccgc cgagctgaaa cgccccgtcg
     6361 aagacctgtt gaaacagttg aaagaagccg gcgtcagcaa aaacagcggc agcgattccc
     6421 tgacgctgga cgacaaacag cttctgaacg cctacctgac caagaaaaac ggcagcaacg
     6481 gcggcaccat cagcatccgc cgcaccaaaa ccgaagtcag caccgttgac ggcgtaaaag
     6541 tcgaaacacg caaacgcgga cgcactgtca acattccttc tgccgaagaa ttggcagcac
     6601 aagtaaaagc cgcccaaacc caagccgcac ctgttcagcc ggagcagacg gcagaagacg
     6661 cggtaaaagc ccgagccgaa gctgccgcac gcgcagaagc ccgtgccaag gcagaagcgg
     6721 aagcggcaaa actgaaagcg gcaaaagcag gcaacaaagc caaacctgcc gcgcagaaac
     6781 ctaccgaagc aaaagccgaa accgcacccg ttgcggcgga aaccaaaccc gccgagccca
     6841 aagaaaaagc cgtcaagccg aaacacgagc gaaacggcaa aggcaaagat gccaaaaaac
     6901 cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa gaacaggcgc
     6961 aacgcgacga agaggcgcgc cgcgccgccg cattgcgcgc ccaccaggaa gccctgttga
     7021 aagagaaaca ggaacgccag gcacgtcgcg aagccatgaa acaacaggca gaacaacagg
     7081 caaaagccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct gccgagaaac
     7141 cgcaggcagc cgcaccggcc gtcgaaaaca aacctgtcaa tccggcaaaa gcgaaaaaag
     7201 aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc aaaggtgcaa
     7261 aaggcgggcg tgaccgcaac aatgcccgca acggcggcga cgagcgcgta cgcggcggca
     7321 agaaaggcaa aaaactcaaa ctcgagccga accaacacgc cttccaagca ccgaccgaac
     7381 ccgtcgtaca cgaggttttg gttcccgaaa ccattaccgt tgccgatttg gcgcacaaaa
     7441 tggcggtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg atggttacca
     7501 tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc ggacacatcg
     7561 gcaaacccgc cgccgccgac gatcccgaag ccttcttggg cgagggcgcg gaagcggtgg
     7621 aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc gaccacggca
     7681 aaacctcgct gctggactac atccgccgcg ccaaagtggt acagggcgaa gcgggcggca
     7741 ttacgcagca catcggcgcg taccacgtca aaaccccgcg cggcgtgatt accttcttgg
     7801 acaccccggg ccacgaagcc tttaccgcta tgcgcgcgcg cggcgcgaaa gcaaccgata
     7861 tcgtgattct tgtggtcgcc gccgacgacg gcgtgatgcc gcaaaccatc gaggcaattg
     7921 cccacgctaa agcggcaggc gttccgattg tggttgccgt caataaaatc gataaagaca
     7981 ctgccaaccc cgaacgcatc cgtcaggaac tgacccaaca cgaagtcatc cccgacgatt
     8041 ggggcggcac ggttcaattc atcgacgttt ccgcgaaaaa aggaacgaac atcgacgcgc
     8101 tgctcgaagc cgtattgctc gaagccgaag tattggaact gaccgcacct gtcgatgcgc
     8161 ccgccaaagg catcatcgtc gaggcgcgtt tggacaaagg gcgcggcgcg gttgccacat
     8221 tgctggttca aaacggtacg ctgaaaaaag gcgacatgct gctggccggt acggcattcg
     8281 gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc ggcccgtcca
     8341 tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac gcgatggtat
     8401 tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa taccgcgacg
     8461 tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat atgggcgaaa
     8521 cccaggccca atctttgtcg gtcatcatca aggcagacgt tcagggctct tacgaggctt
     8581 tggcgggcag cctgaaaaaa ctgtccgccg acgaggtgaa agtgaacgtg ttgcacagcg
     8641 gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc gcgttcatta
     8701 tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat gaaaacgtgg
     8761 aaatccgcta ctacaacatc atctacgatg ccatcgacga cgtgaaggcg gcgatgagcg
     8821 gcatgctttc tcccgaaaag aaagaacagg ttaccggtac ggtcgaaatc cgtcaggtca
     8881 tctccgtttc caaggtcggc aacattgcag gctgtatggt caccgacggc gtggtcaaac
     8941 gcgattccca tatccgcctc atccgcaaca acgtggtcat ccacacgggc gaactggctt
     9001 cgttgaaacg ctataaagac gacgtaaaag aagtccgcat gggcttcgag tgcggtctga
     9061 tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc gacatcgtcg
     9121 aagttgcccg caccctgtaa ttcctttgca aataaaatgc cgtctgaagc gttcagacgg
     9181 caacgaaacg ggttctgtat catacagaac ccgttttttg tcgcaaatcg gcttcagcga
     9241 ccctcctgcc ttatcccgat ttgaatctga cttgccatac aaacaggctt cagacggcat
     9301 tatttgcccg ccaaacgtat cccgatcttc tccgcatatt ccctgcgttc ggcgcggctg
     9361 gtttccgggc ggtgcgtatt gagcgacgac catttccaat gactgcgggc tttgttgagt
     9421 tcgggcggga gtttggcggc atcccacggg gctttgcggc tgtgcagctc gatatccgac
     9481 tgtgccgcgt gtccgcgcgt ttgcaggacg tggagcagat cgagggcgcg ggcggcgagc
     9541 agggtcaggg tttcagggtc ggtgtgcagg gtttgacggc cggcgagttt gtcggaaatg
     9601 gtgcgggtat tgggcaggat gccgcccaaa aagccgccga ttgccgtacc caatccgagc
     9661 gagccgccga gtgtggcgat gtccagcccc aagccgatga gcgcgccggt tgccgcgccc
     9721 gtgccggtgc ggatgccgta ttgtttgagc aattcgctgt cgaacgggtc ttggcggaag
     9781 gcttgcggca tccagtcgcc gccgtcgatt tcgctgtggt agaaacggta aagggcaaac
     9841 agccgctgct gcatctgccg ttcgagttgg cgtatttccg cctgcatggt ttgcagcacg
     9901 gtggcggtat cctcgttttc gtccacttcc tgcctgaagg cggcggcatc gagcaggaaa
     9961 tcggcgattt cgcggcgcgc ttcgccgtcc agccgctgcc attcgcgccg gcgcatggct
    10021 gtcaggcggt caagtgtgct gcgttcgggc aacatggtgg cgaggttttc ccacaggcgc
    10081 agttcgcctt caaaatcaaa ggcgacggtg tcgaaccctg cgaaaacgtg caggtttctc
    10141 ctcgccagca tggttgtcca tgattcggga agctgtccgc cggtaaagtt gaacacgggc
    10201 ataaccggtt tggcacacca tgaaaggatg gtcaattcat cccgatattt gtcgaggacg
    10261 ggttcgcgcg cgtcgatgac gtacattgcc atatcgcttt gcaaaacctg ccgcagcact
    10321 ttggcttctt ggttgaaatc gtgatgcgcg ccgtggctgc cgagaaactg ttgcagccgc
    10381 tcgatgccgt ctgaacgatt gtccgtatgg ttttccagcc attccaaaac accgcccgcg
    10441 tcttcgagtc cgggcgtgtc atacaggaaa accaacatgt ccgcgccgtc gctgatggcg
    10501 gcttcttcga catggcgtgt ggtggacggt gcgtttctga cttcgccgaa gccgctgtcg
    10561 cgcaacaggg tgcgcaggag cgaggttttg ccggtgttgg tgtgtcccac gacggcgagg
    10621 gaaaggggtt gtttgttcat gatgtttttg aagaatggat tttcagacgg tcttttttca
    10681 gaatggcggc ttaacagaac atttcaagtg agtttattgg tctttcaaac ggccttcctg
    10741 cgccaccctg tcaggctcaa gccacgccgc gccgcattcg gtcagcgcgt tacgccaatg
    10801 ttccagcttt tccgaaaggt cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc
    10861 gccgccctgc gccgcttccg aaagccgcac aatctgccgc agcacgcccc ggtccggcac
    10921 agtttgggcg cgtacgccga taagcagttg cgccggtttc tgcttcagct ctgtctccag
    10981 cgcggcaacc tgttcccgat tggcggcaac gcccttatcc agccattcct gcgccagcct
    11041 gccctcgaac cattggccgt cctgccactc ggtctccagc atgagcgccc atttcggcgc
    11101 atcgttcaag acgattttcg gcgaaacggc ggacacggtt tcccgacgcg tatccgcatc
    11161 ggtgattttg ttctgccagc ggcggatgac cgcctgataa taggtttttt ccaaatccaa
    11221 tccgttttcg cttgttttca aaaggatttt acacactacc caagccaaga ggcgcggcag
    11281 gatgccgtag cagacgatac tgccgaccag cagccccgac caagcccgcg catcggcaat
    11341 attgccgttc agacgacctt cgatgaccgc ccgcgcatcg gggacaggga aaccgagttt
    11401 cgacggcagc catgccaaca tttccaccgc gcgtaccgaa gcggcattgc tcaacagcgt
    11461 gctttcccag ttgaacgtat attgccgcac caaaagcagc agcaataccg acaccagcat
    11521 tccgagcagc gtgcagagcc acaagctgtg cgccgttgcg cctattttcc atcgtaccga
    11581 aggttgccgc cactggtccg catacagccg caacaccgcc tgatttacag ggcctttgcc
    11641 ccgaaaccac gtcgccggac tgctgaaaaa ccgccccact ttcacgcgca ggaacaacgt
    11701 tgccaaccat actgccagca tcagcgtatt catgcccaac actcccgcca aaactaaaaa
    11761 gaaattcagc ccctgattgt ccatcagaag ataagtgcct gaaaatccgg cggtaaacat
    11821 catcgatgcc accaccaccc ataaccagaa cgaccccgca cgcacacgtt ccaacgtgtc
    11881 ccgcagcata cggtccctgt cgatcatctc cgcccgacgg atgatttttt cctccgtact
    11941 gccgtccacg cggcgcaaag cctccgtcgc ctgcacggga tcgccgctga aaataaaacc
    12001 gccttcatcc aaaatacgga ccagctcaac cagttttcgg gatggattca acataaaatg
    12061 ccgtctgaaa ataaaaaaca aattttaaca cacgcatttt caagaatatt cacagtgtag
    12121 gcaaagagta aatcccacac agaggcaaaa gtatcggcgt aaactgactg cctctacttt
    12181 cccgaaagat tgtgcgatgt atgcaggcga acgcttcaat acttacagcc atttgagcgg
    12241 tttgattctg gcggcggcag gtttgatgct gatgctgctg aaaaccatag gacacgggga
    12301 cggctaccgt atcttcagcg tatcggttta cggcatcagc cttcttctgc tctatttgag
    12361 ttcctcgctg taccacggaa ttgcagccgg aaaactgaaa agcattttga aaaaaaccga
    12421 ccactgcatg atttatgtgc tgattgccgg aagctacaca ccgtttgcac tggtttcttt
    12481 gagaaacggg ccgggctgga cggtattttc actgtcctgg ctgctggcgg ctgcaggaat
    12541 cgcacaagaa ctcaccatcg gacggaaaag cgaaaaacgt ctgctgtcta ttgcgattta
    12601 tatcgtaatg ggctggatgg tcttggcggt aatgaaatcc ctgacagcct cactcccgcc
    12661 ggcaggactg gcttggctgg cggcaggcgg tatgctgtac agcgtcggca tttactggtt
    12721 tgtaaacgat gaaaaaatcc gacacgggca cggaatctgg catctgttcg tattgggcgg
    12781 cagcataacc caatttgtca gcgtgtacgg ttatgtaatc tgaatgccgt ctgaaacaca
    12841 aaaacctccc aaacctgaag gcagggaggt tttctctttg ccgggcatta ttgtttatcg
    12901 tggaactcgt ggaattcata ctgataggat aaatcctgtc ccgccttttt ctgcatcagg
    12961 tagtcatcat aaatggcgcg gatttcctta cgcagcaaga ataaggcaat caggttgggg
    13021 ataaccataa agccgttgaa catatcggac agattccaaa ccaaatcgac tttgccgagc
    13081 gtacccaaaa cgatagcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg
    13141 aaaagaaaac ggatgttgga ctcgccgaaa taataccagc cgataatggt cgtaaaggcg
    13201 aagaaggtca gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag
    13261 gcaaattgag taaccgccgc gccctgttcg cccgaaaggt tggcatcggt cagcaggata
    13321 atcaatgccg tagccgtaca taccaaaatc gtatcgataa atacaccgac aaatgccgtc
    13381 ataccttgct gaacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg agtcgaaccc
    13441 atacctgctt cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcacgcata
    13501 ccgatacccg cagcaccgcc caaaacggcc tcgggattga aggcggcggt aaagatgtgg
    13561 ttgaacatcg gcacaatatg gtcggaaaat tcaaacagga taacgacggc gcacaaaata
    13621 taaacaaccg ccataaacgg cacgacaaac cgggcaatat tggcaatacg gttcacgccg
    13681 ccaatcacaa ccatgcccgc aaggacggca aacacaatac cgactgccaa agaaggcaca
    13741 tcaaatgcaa tggtaacggc agaagcgatg gaatttgcct gagtcgcatt gccgataaag
    13801 cccaacgcga caatcagcgc gatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg
    13861 ccgattttcg gagtcagacc gtgcgtaata taaaacgccg gcccgccgat gtatttgccg
    13921 tggctgacga cgcggtattt ctgcgccagc agtgcttccg caaaaatcgt ggacatcccc
    13981 aaaacggcag aaagccacat ccaaaaaatc gcacccggcc cgcctgcggt aatggcggtc
    14041 gccacaccgg cgacgttgcc cgtaccgatt tgcgcggata tggcaaccgc caacgcctga
    14101 aactgcgata aggacttatc gtctttatca cctttggcaa acaagccgcc gaatacggat
    14161 ttgaatcccg cgcccagctt ggtaatctgc ggcgcaccaa gatacagcgt aaaaaacagg
    14221 ccgataccca aaagcgcata aatcagcagg tagtcccaaa gaaaccgatt gactgtaccc
    14281 accagaacag acaatatatt ttccataaaa taaaccttat cttacgatta aaatgactgt
    14341 tttccaaaag acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat
    14401 gccttaaatt gtcaataatc cgagagaagc tgtgccagca taccgtaaaa tatcgttaat
    14461 taaaatattt ctttattttt aagcggaaag cggaggaaat cgttttcaga cggtatcgac
    14521 aacggcacgg cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc
    14581 gtaaaaaatc tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg
    14641 ccatagcaag tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa
    14701 aatcttcacc cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc
    14761 cggcgcaaat ccgcgcctgc gtctggcttt ggaaaaagca gccgaaaaca atatgcccaa
    14821 agacaatgtg caacgcgcca tcgacaaagg tacgggtaac ttggaaggcg tggaatacat
    14881 cgagttgcgc tacgaaggct acggcatcgg cggcgcagct ttgatggtgg actgcctgac
    14941 cgacaacaaa acccgcaccg ttgcggacgt acgccacgca tttaccaaaa acggcggcaa
    15001 cttgggtacc gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga
    15061 acccggcgtt gacgaagacg agctgatgga agcggctttg gaagccggtg cggaagacgt
    15121 ggttaccaac gacgacggtt ccatcgaagt cattaccgcg ccaaatgatt gggcgggcgt
    15181 aaaatccgct ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc
    15241 ccaaaacgaa accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc
    15301 gctggaagac ttggacgacg tgcaagacgt ttacacttcc gccgtattga atctggactg
    15361 atacgcagca cagcagacat acaacaaaat gccgtctgaa cctttcagac ggcattgatt
    15421 tatttagccc ttgcccacgc ccaccaaacc gtcaggacaa ccgccaaaaa atacgccgcg
    15481 ctccaaacag gcaaagcaac ccctaacaga taatccggtt cggcacaatt tccgaacccg
    15541 cgcacaacag gctcgaacca atcaaacaaa ggccagccct tcaatcgaaa cgtccacggc
    15601 gcgccgcacg aaggagccgc acccggcggc agcgactgca accacaactg atatgccgca
    15661 acagaaatac ccgtaacggc gggaatactg ataaacactg cgcctgacag tccgcccacc
    15721 ctgcccttgg gcttacacgc cagcacaact gccgcacaca atgcggttgc caaaatacat
    15781 aaccgctgac tgatgcacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa
    15841 gaaccggcaa atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac
    15901 ggggtcattt tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc
    15961 ccgagatggc aagggacgaa tcgacaccgg cacaggtgcg gacgatttcg gttttcccga
    16021 tatgcacaaa acctgacaaa ggacaaaacg gcgatttgtc cgcacaatgc ccgaaaaaat
    16081 acatccggag gatttgaatt tgaatttcgg caaatttagc gaatcaaaag caacagccga
    16141 taaaaaaatc aaaagtttat ttcaatgttt caacacacag gacgacacat aaagcaccgc
    16201 cctatgtgcc gtcctgattt ggaaggggtt acacccctcc caaatatagt ggattaacaa
    16261 aaaccggtac ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgccgc
    16321 cttgtcctga tttttgttaa tccactataa agtctgatcc tgccgcccta aagggcaggg
    16381 tttcaaccga aaaggaaaca cgatgaaagc atatgatttt ttgaataatc ggattgacgg
    16441 gtttttgagg gaatttgtta ccggatagcc atcgggcaag ttttttgcaa aatttgaatc
    16501 ggtcgggtaa attttcaaaa aataattgac agcagataag aaacggcgga taattaccgc
    16561 cgttgagttg cttgatgcag cttgattttt ctcctctact tctcctttgt agacttggca
    16621 cacattctat tgaatgtgtg cattttttat ctgaagcaac aagcctctgt gcgtgatgtt
    16681 gttatgtttc atttagatgt caaaccgcat acccggtctg aaatattcaa tccaaatcca
    16741 aaaccggatt ttctttgact tcctccatca caacataact cctactctcc gaagcggcag
    16801 gcagttgcaa taggatattg ccgagcatat cccgataggc agacatatcg ggcaaacgta
    16861 ctttaatcag gtagtcgtat tcgcccgaca ccagatggca ctccataatt tgcggaattt
    16921 tcagcacttc ttttttgaaa tcttcgaaaa tattgcccga tttggattgc agcttcagct
    16981 cgacaaaaac caatggcggt ttgcccaaca gatggggatt gagatggacg tgatagccgg
    17041 aaatataatg ttcccgctcc aaacggcata ccctctctgt aacgggcgtg ctggacaacc
    17101 ctaccttctc ggcaagctcc gtcatcggga tacgggcatt ttgctgaaga attttgagga
    17161 tgcggaaatc gattttatct agttctttca tttggattac cttgtattta ttattgattt
    17221 taacaaatag agtataaatt tgggaaagca attatatcag gaaaagcaaa ccgccttcct
    17281 acctgaaaac tgctgctctg gcttgaagac acaaggttct ttaatatttt aaaagccttg
    17341 ccgttggatt ataattcccc aattgatttc ttaattttgc taataaacac ttgtttggta
    17401 aggaatgaat ttatgcggcc tttgaacgtg cagatcaggt tgggcaacct taggcacaat
    17461 tatcggattt tgaaggaaat gcacggaggc aagctgttgg cggtagtgaa ggccgacgca
    17521 tacggacacg gtgcggtcag atgtgctttc gcgctggcag acttggcaga cggctttgcc
    17581 gtggcgacaa tcgacgaggg aatcaggctg cgggagagcg gcattaccca tccgattgtc
    17641 cttttagaag gcgtatttga agcatcagag tacgaagcgg tcgaacaata ctcgctttgg
    17701 ccggcagtcg gaaaccaatg gcagcttgag gctttgcttt cccgccattg gaaaaaacct
    17761 gtcaaggtct ggttgaaaat ggattcggga atgcaccgta ccggattttt ccctcatgat
    17821 tacgcttcgg catatgcggc attgaaacag tcggaatatg tggacagtat tgtcaagttc
    17881 tcgcatttct cctgtgcgga cgaacctgaa agcggtatga cggaaataca gatggaagca
    17941 ttcgatttgg gtacgaaagg gctggaaggc gaagaaagcc ttgccaattc ggcagctatt
    18001 ttgaatatcc ccgaagcacg cagggattgg gggcgcgcgg gcttggcgtt atacggcatt
    18061 tccccgttcg gaggcagcga tgacaggctg aaacccgtga tgaggctttc aacccgtatt
    18121 ttcggcgaac gcgttttaca gccgcactcc cctatcggtt atggcgcaac attttatacc
    18181 agtaaatcta cgcgcgtcgg tctgattgcc tgcggttatg cggacggtta tccgcgccgc
    18241 gctccaagca attcccccgt cgccgtcgac ggtaaattga cccgggtcat cggcaggatc
    18301 tctatggata tgatgactat cgagctggac gcttcgcaag aaggtttggg acacgaggtc
    18361 gaactgtggg gcgatacggt caatatcaat accgttgccg aagcggccgg aaccatccct
    18421 tacgaattga tgtgcaatat caaacgtgca aaattcactt atatcgagta atcgagtcca
    18481 aacgaaaatc ccgtctgaag cttttcagac ggcataaatg cgttttgaca aaatcagtaa
    18541 aagacattgc caaatagaaa atactgataa tgcccacaat ctgaaacccc atccttcccg
    18601 caaaaacgga aaatcaaaat cagaaaccta aaatttcgtc attcccgcgc aggcgggaat
    18661 ccggtttttt gagtttccgt catttctgat aaattctcgt cgtttttcat ttctagattc
    18721 ccgcatgcgc gggaatgaca aaattaaagt ttcagaattt atttgaacaa caaactagat
    18781 tcccgctttc gtggggatga cggcggatag gttttgttgt ttcggataaa ttcctgttgt
    18841 tttttggttt ccagattccc acctttaaaa gaatgacgta agaaagcaga aataagaaca
    18901 cggggatttt ttaaatttgc aatattcttt tctaaagaca ttttacccaa tgcccataaa
    18961 caaaaatgcc gcctgaagcc tttcagacgg cattttccca tcaaaaccgc aatcagtttt
    19021 tcatcgattg aaccggtgcg ggaatcctgc cgccccggtt gacgaacact tcgcacgaac
    19081 cttcttttgc cggcattaca ggcgcgtagc ccaacagacc gccgaactcg acgctgtcgc
    19141 cgacggtttt gcccgttacc ggaataatgc gcacggcggt ggttttgctg ttgatcatgc
    19201 cgatggcggc ttcgtcggcg atgatgccgg aaatggtgtg cgcgggcgtg tcgccgggaa
    19261 cggcaatcat gtccaaacca acggagcaga cggcggtcat ggcttcgagt ttgtccagcg
    19321 tcaacacgcc tgcctcggcg gcggcaatca taccttcgtc ttcggaaacg gggataaacg
    19381 cgccgctcaa accgccgacc gcgctggaag ccatcatgcc gccctttttc acggcatcgt
    19441 tcagcaatgc caaagctgct gttgtgccgt gcgtaccgca gacgctcaag cccatttctt
    19501 caagaatgcg cgccaccgag tcgccgacgg cgggggtcgg tgccagcgac aaatcgagaa
    19561 tgccgaacgg gatattcagc atttttgagg cttcgcggcc gatgagttcg cccacgcggg
    19621 tgattttgaa agcggttttc ttcacgactt cggcgacctc ggtcaggctg accgcgtccg
    19681 aattttccag cgcggctttg accacgcctg gaccggatac gccgacatta atcacagcat
    19741 ccgcttcgcc cgagccgtgg aacgcacccg ccataaacgg attgtcttcc accgcgttgc
    19801 agaacacgac gattttggcg cagccgaaac cttcgggcgt gatttcagcc gtgcgtttga
    19861 tggtttcgcc tgccagcttg accgcatcca tattgatacc ggcacgcgtg ctgccgatat
    19921 tgatggagct gcacacgata tcggtagttt tcatcgcttc gggaacggaa cggatcaaca
    19981 cctcatccga aggcgacata cctttttgca ccagcgcgga aaagccgccg ataaaggaca
    20041 cgccgatggc tttggctgcc ttgtccaaag tctgcgccac gctgacataa ctgtcggctt
    20101 tggtcgccgc cgcgatttgg gcaatcggcg taacggaaat gcgctgattc acaatcggca
    20161 cgccgtattt ggcggaaagg tgtttcgccg ttgccaccaa gtctttgccg accgtagtga
    20221 ttttgttgta aatgttttgg tttaacacgt cgatgtcggt gctgatgcag tcgtgcaaat
    20281 caatgccgat ggtaatggtg cggacatcaa aattctggtc ggcaaccatt ttgacggttt
    20341 ctaaaatttc gccggattgg atactcatca cattcctccg actcaaatgc ggtgcatcgc
    20401 ttggaagatt tcttcgtttt gcatacggat gtcaagcgcg agttttttgc tctcttccgc
    20461 aaacaaatcc aaaatctcct gccgcgattt agggcatttc gaggtatcca ccaagataat
    20521 catagtaaaa aaatcgtcca tcagctgttg gctgatattg aggatgttga ttcggttttc
    20581 cgccaaaatt ttggaaacat cgtacacgat gccgacgcgg tctttaccga tgacggtgat
    20641 gactgaattg ttcacaggct tactccttgc agatatccgt taaaatccga aattatacca
    20701 ccgtcggatt ttgaagaata ttgtcaacaa tatatacata caaaatgccg tctgaaacta
    20761 tttcagacag catcaagatt cagggttcga ttaaataacc atccttatcc cattgggttt
    20821 tcctgaccaa cttgtcatcc tgataaacag cctcgctctt tttagaacca tcttcatacc
    20881 actccaaaac caccccgttg cgtttatggt ggcggataga cagttccgag agtaatcggc
    20941 cgctttcatc ccaagtcaga attttggcag gctcatcgtt gaccataacc atttccgtct
    21001 tgatacttcc atcggcatac cattgcttcc atacgccgtt cgccttattt tgcttaaact
    21061 ggatttcgct ttccttgccg ccgttacggt aataacggta tcccgtaccc tcactcaagc
    21121 catttttata aggcataacc gcagattttt taccgttcgg ataccagttg acccattccc
    21181 cgtccggctt acccttgctg aagcccccgc catttttttc tgaccattaa aatgccacaa
    21241 aatcaacata ccgttttgca gggtaggcac aaaagatttg atttgcgttg aagcaacgat
    21301 ataaggttcg gaatatttct tcatcgacgg ataataaaaa tcctgcgcgt gtgcgatacc
    21361 cgccgccaca ctatattgcc tgatataagc ggcagaagac atcgtcgccg tcagtttccc
    21421 gttctgatta aaataaacag aataggtctg cgccggcaaa gcggccgaaa aacccaacag
    21481 tacgattgaa aatacaatcc gagataattt cttcattatg atagcgatat aaaaaacaat
    21541 taacttataa acaatatggt aaggatttct caaccaagcg tcaaacccga aacaatgtat
    21601 cgtacaaatg ccgtctgaaa acaaatcgtc ttcagacggc atttccctcc aactcactct
    21661 tcgcccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc gctggtttcc
    21721 aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt atgcccgatt
    21781 tccaccagca atacaccttt aggattcaga aactttgccg cattcagaag aatctgcctg
    21841 gtggcatcca acccgtccgc cccgctgccc aacgccaatt ccggttcgtg caaatattcc
    21901 tcaggcaaag ccccaaccga ctctgcatcc acataaggcg gattggaaac aatcaaatca
    21961 taagtgcctt ccagtccctc aaacaaatcg gtatgaataa gctgaatacg ctcttccaga
    22021 ccataatctt caatattgat ccctgcaact tccaaagcat ccaaactcac atcaaccgca
    22081 tcaatttggg catcaggata atggtgcgcc atctgaatgg caaggcaacc gcttccggtg
    22141 caaagatcca aagcattatg caccagctca tcgtattcta tccaaggtcg aagtccgtca
    22201 cccagcaatt cataaataaa agagcgcggt acaatcacgc gttcatctac ataaaaatca
    22261 aactccccct gccaggcctg gtgtgtcaaa taagcggcag gaatgtgttc gacagcacga
    22321 cgctcaataa ccgccagcac ttcctctttt tcagcttcca agagttttgc atcaagatat
    22381 ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataaac cgcttcatca
    22441 tgcgcattat ctgttccatg accaaaaaag agccctgcct cattaaaacg gctgactgca
    22501 aaacgtaaaa catcacggat agtcgtcaat tcttgtgcgg cctgattaaa cataatatga
    22561 accattctgc gtatagatgc ttttaattat aacagaaaca acaagcaaac cttttcatat
    22621 cgccaaataa ccactcaatc tacccataca actacataaa tgccgcgcga agaccattgc
    22681 ccgaacggaa acggcgatgg cggacggtat gggcaacctg ataggctggg aagaaacggg
    22741 gcttgttgag ggcaagcagt ggataaccgc aaaagacgac aaggtgtccg atgtctgcaa
    22801 tgccaacggc gggatgggcg taatcggttt gcacgagcct ttctcccacg gtgcgttgac
    22861 gataccggag catccaaatg gcaggtgcgc ggttgttccc gttgtcgggc ggacaggcaa
    22921 gctggcgaaa ggcagccgcg cgatggaaac gggcgatgga cggcaggtga ggttgtttcc
    22981 gtatcgggcg gcgaattggg ggaatttgct gaaacaaagg agcttcgtaa agcggctatg
    23041 cagtatgcgc gtgataactt tgtcggcaaa agctatgtca atgaaagcag cgggcatgaa
    23101 ctgaaggtaa cttggcaagg tgtgaaacac gctgcgtcaa aatcgaacag catcgaactt
    23161 tcccttatgg tcgcgcttga taaagttttg atgaatgcaa aatatgaagg aagttttcct
    23221 aacaaattag ggagggatca tattattgcg gtacacaaat actcaaccgg tgtcagattt
    23281 ggcaataaag tcttaaaaat caggataata gcccgtgaga aatttgatgg gataaaacat
    23341 tacgatcatt ttattttgaa agacaaatga aaaaaccctg tccagtatat ctgggatagc
    23401 ttatggaagc atttaaccca gcctcggata aggctttaaa tccatcatgc catttatacg
    23461 g
//
LOCUS       DAXFHF010000030        23368 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXFHF010000030 DAXFHF010000000
VERSION     DAXFHF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23368
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.030"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..400
                     /locus_tag="ACFIRM_001094"
     CDS             <1..400
                     /locus_tag="ACFIRM_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3028739.1"
                     /translation="KEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLK
                     YRKDDHFSVLRGIGGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVPKKA
                     EAISNKLFFTHHPKRQTEFAVGQIRQGRAE"
     gene            528..1823
                     /gene="hisS"
                     /locus_tag="ACFIRM_001095"
     CDS             528..1823
                     /gene="hisS"
                     /locus_tag="ACFIRM_001095"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC3028740.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            1824..2453
                     /locus_tag="ACFIRM_001096"
     CDS             1824..2453
                     /locus_tag="ACFIRM_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC3028741.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALMILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            2603..4060
                     /gene="der"
                     /locus_tag="ACFIRM_001097"
     CDS             2603..4060
                     /gene="der"
                     /locus_tag="ACFIRM_001097"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC3028742.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFENIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            4091..4990
                     /locus_tag="ACFIRM_001098"
     CDS             4091..4990
                     /locus_tag="ACFIRM_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC3028743.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            5042..5459
                     /locus_tag="ACFIRM_001099"
                     /pseudo
     CDS             5042..5459
                     /locus_tag="ACFIRM_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5526..6092
                     /gene="dcd"
                     /locus_tag="ACFIRM_001100"
     CDS             5526..6092
                     /gene="dcd"
                     /locus_tag="ACFIRM_001100"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC3028744.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6158..6862)
                     /locus_tag="ACFIRM_001101"
     CDS             complement(6158..6862)
                     /locus_tag="ACFIRM_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC3028745.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            7006..8201
                     /locus_tag="ACFIRM_001102"
                     /pseudo
     CDS             7006..8201
                     /locus_tag="ACFIRM_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            8227..9180
                     /locus_tag="ACFIRM_001103"
     CDS             8227..9180
                     /locus_tag="ACFIRM_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC3028746.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9285..9608)
                     /locus_tag="ACFIRM_001104"
     CDS             complement(9285..9608)
                     /locus_tag="ACFIRM_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028747.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(9781..11139)
                     /locus_tag="ACFIRM_001105"
     CDS             complement(9781..11139)
                     /locus_tag="ACFIRM_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC3028748.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(11497..13197)
                     /gene="recJ"
                     /locus_tag="ACFIRM_001106"
     CDS             complement(11497..13197)
                     /gene="recJ"
                     /locus_tag="ACFIRM_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC3028749.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(13249..13980)
                     /locus_tag="ACFIRM_001107"
     CDS             complement(13249..13980)
                     /locus_tag="ACFIRM_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028750.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTDRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILVYAPLVDIGCG
                     DRGAGLAYGSDCSREFLRQMNGNAV"
     gene            complement(14027..14533)
                     /gene="yjgA"
                     /locus_tag="ACFIRM_001108"
     CDS             complement(14027..14533)
                     /gene="yjgA"
                     /locus_tag="ACFIRM_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC3028751.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADEGALTQFMSDFPNAGAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            14687..16018
                     /gene="pmbA"
                     /locus_tag="ACFIRM_001109"
     CDS             14687..16018
                     /gene="pmbA"
                     /locus_tag="ACFIRM_001109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC3028752.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16317..16520)
                     /locus_tag="ACFIRM_001110"
     CDS             complement(16317..16520)
                     /locus_tag="ACFIRM_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC3028753.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            16765..17067
                     /gene="clpS"
                     /locus_tag="ACFIRM_001111"
     CDS             16765..17067
                     /gene="clpS"
                     /locus_tag="ACFIRM_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC3028754.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17069..19360
                     /gene="clpA"
                     /locus_tag="ACFIRM_001112"
     CDS             17069..19360
                     /gene="clpA"
                     /locus_tag="ACFIRM_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC3028755.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(19400..22498)
                     /locus_tag="ACFIRM_001113"
     CDS             complement(19400..22498)
                     /locus_tag="ACFIRM_001113"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC3028756.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILNRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQADSPH
                     PLKTATIFSFAANEEQNAVGEIVDENFEPEAMDSSAKEFLQAVINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(22730..23299)
                     /locus_tag="ACFIRM_001114"
     CDS             complement(22730..23299)
                     /locus_tag="ACFIRM_001114"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3028757.1"
                     /translation="MVWKTLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSG
                     YVPSEGKMTEYIVNDILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQ
                     VLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSV
                     SEGLPHEIALRRKQYEYYREQLLAFPKAA"
BASE COUNT         5591 a         6598 c         5830 g         5349 t
ORIGIN      
        1 taaggagcag atgcgcgcgt cgtttgagga tatgcggcgc attatgcctg atttgggttt
       61 tgaggcgaag ggctatgccc tgtctttcga gcagctcgcg cagttgaaaa tccccgtcat
      121 cgtgtatctg aaataccgca aggatgatca tttctcggta ttgcgcggca taggcggaaa
      181 tacggttttg cttgccgacc cgtcgccggg tcatgtttcg atgagcaggg cgcagttttt
      241 ggaggcttgg caaacccgtg agggaaattt ggcaggcaag attttggcgg tcgtgccgaa
      301 aaaagcggag gcgatttcaa ataaattgtt tttcacacat catcccaagc ggcagacgga
      361 gtttgcagtc ggacaaatca ggcaagggcg cgcagagtaa gtcaaatgcc gtctgaaaac
      421 tgtttcagac ggcattttta cccgtccgca aaccatctcc atcctcacaa gcccttaaaa
      481 atccgctata atcgcccgca atttgatttc agcaccccct ttttgaaatg gcacaaaaaa
      541 tccaatccgt caaaggcatg aacgaccttc tgcctgtcgg gcaaaaagat ttcaaactca
      601 cggctgtgtt ttggcaggcg tttgaggata cggtaaaccg ttggacgcga gcttacggtt
      661 atcagcaaat ccgtacgccg attgtcgagc aaaccggttt gtttgtccgc tccatcggcg
      721 aggaaaccga tgtagtcggc aaggaaatgt acaccttctc cgattcaaac gattctttga
      781 gtttgagcct gcgtcccgaa ggtacggcct cttgtctgcg tgcggtggtc gaacacaacc
      841 ttctgtacaa cagcccgcaa aagctgtggt atatggggcc gatgttccgc cgcgagcgtc
      901 cgcaaaaagg acgctaccgc caatttcatc aggtcggtat cgaggctttg ggttttgaag
      961 ggccggatat tgatgcggaa atcatcgcaa tgtctgccga cttgtgggaa aaattgggta
     1021 ttcgcgaata cctgactttg gaaatcaaca gcttgggcaa ccgtgaggaa cgcgcggcac
     1081 accgtgcggc attggttgaa tatctgaccc gctatgaagc gcaactggac gaagacagca
     1141 aacgccgtct gaaaaccaat cctttgcgcg ttttggatac gaaaaaccct gatttgcagg
     1201 aaatctgcaa tgccgcgccg cgtttggtag attacttggg cgaggcttcg caaaaccact
     1261 atgcacgctt caaggcgatg ttggacggtt tgggtattca atatattgaa aattcgcgct
     1321 tggttcgcgg tttggattat tacaatcaga cggtttttga gtggacgacc gacaaactcg
     1381 gcgcacaggc gactgtgtgc ggcggcggac gttacgacgg tttgattgaa gaactcggcg
     1441 gcaagcctgc gccgtccatc ggctttgcga tgggcatcga acggctgctg cttttggtga
     1501 gcgaatacgg ttcgctggaa gtgaacgccg cgcctgatgt ctatgcaatg caccaaggcg
     1561 aaggggcgga cttgcaggtg atgaaatacg cacaagcctt gcgcgcgcaa ggtttcaatg
     1621 taatgcagca ttccggctat caaagcctga aggcgcaaat gaaaaaagcc gacaacagcg
     1681 gcgcgcgctt tgccctgatt gtcgcgcaag acgaactggc ggacggtacc gttacgctca
     1741 aagacatgaa cggcgcacac ggtcagcaaa ccgtttccgc caccgatttg acagatactt
     1801 tacaacaatg gaagaacgca taaatggcag cccatctcga agaacaacaa gagttagaca
     1861 actttaaata tttttggaaa accacgggca aatggctgtt tgccctgatg attttggcgg
     1921 cactcggcta cttgggatac acggtttacc aaaaccgtgc ggcttcccaa aatcaggaag
     1981 cggcggcggt gctggcaaac atcgtggaaa aggcgcaaaa caaagccccg caaagcgaaa
     2041 tcaatgccga actgtccaaa ctccaacaaa gctaccccca ttccatttcc gccgcccaag
     2101 ccacgctgat ggcggcggca accgaatttg acgcgcagcg ttacgatgtt gccgaaggtc
     2161 atttgaaatg ggtgttgtcc aaccaaaaag acagcctgat tcaggcgttg gcggcgcagc
     2221 gtctgggcgt tgtgttgttg caacaaaaaa aatacgatgc cgcgcttgcc gcactcgaca
     2281 cgccggttga ggcggacttc gcccccctgc tgatggaaac taaaggcgat gtttatgccg
     2341 cacaggaaaa aagccaggaa gccttaaaaa actacggaca ggctttggaa aaaatgcctc
     2401 aagattctgt cggtcgcgaa ttgcttcaaa tgaaactcga ttcgctgaaa taaataccgt
     2461 ctgaaatgcc gctctctctt cagacggcat ttcagacaac cccatcctac ggcaattccc
     2521 gtaaaaccat ccgccgtgtc ccaacggaca cctgctcgtg ttcagttcta ccgaacggca
     2581 cttgacacag aaagacccaa tcatgaaacc aaccatcgcg cttatcggcc gccccaacgt
     2641 cggcaaatct accttgttca accgtttgac gcgcaccaaa gacgcgctcg tgcatgacct
     2701 gccgggtctg acccgcgacc gccattacgg acacggcaaa gtcggcagca aaccttattt
     2761 tgtcatcgat accggcggtt tcgagccggt tgtggacagc ggcattttgc acgaaatggc
     2821 aaaacaaacc ttgcaggctg tcgatgaagc cgatgcggtt gtgtttttgg tggacggccg
     2881 taccggttta acaccgcaag acaagattat tgccgaccgt ttgcgccaaa gtccgcgtcc
     2941 cgtttatttg gccgtgaata aaggcgaggg cggcgacagg gccgtacttg ccgccgagtt
     3001 ttacgagctg gcattgggcg agccgcacgt tatttccggc gcgcacggcg acggcgtgta
     3061 ttacctgatt gaagaaattt tagaaaactt ccccgagcct gaagccgaag aagccgatgc
     3121 aaaacatccc gtttttgccg ttatcggtcg tccgaacgtc ggcaaatcta cgctggttaa
     3181 cgccattctc ggcgaaaaac gcgtcatcgc cttcgatatg gcaggcacaa cgcgcgacag
     3241 catccacatc gatttcgagc gcgaaggcaa accgtttacc atcatcgata ccgcaggcgt
     3301 gcgccgtcgc ggcaaagtgg atgaagcggt ggaaaagttc tccgttatca aagccatgca
     3361 ggcggttgaa gcggcaaacg ttgctgtttt ggtattggac gcgcaacagg acattgccga
     3421 ccaagatgcg acgattgccg gttttgcttt ggaagcggga cgcgcgctgg tagttgccgt
     3481 caacaaatgg gacggcatca gcgaagagcg gcgcgaacaa gtgaaacgcg acatttcccg
     3541 caaactgtat ttcctcgatt ttgccaagtt ccactttatt tccgcgttga aagagcgcgg
     3601 tatagacggt ttgtttgaaa acattcaagc tgcctacaac gcggcgatga ttaagatgcc
     3661 gacgccgaaa atcacgcgcg tattgcaaac cgccgtcgga cgccaacagc cgccgcgtgc
     3721 cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc ggcatgaatc cgccggtgat
     3781 tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc tatacgcgct atctgaccca
     3841 gaccttccgc aaagccttca acctgcaagg cacgccgttg cggattcaat acaatgtttc
     3901 ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa aaaccgctgc gccgcgtcag
     3961 cctgagcaac cgtattgaga aacgcgaagg ccgaaaggaa gagaaaaacc gcttcaagaa
     4021 gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa ttcccgacat ttcaaacaat
     4081 aggaaacatt atgtggttca agcagattag tttttatccg ctcaacaaag aaaagctgcc
     4141 tgaggcggac gtacttgccg acaaacttgc tgaagctgaa tttacccatt gccaaggttt
     4201 ggactggttc agcgaaggct ttaccgcacc ggtttctttt tcgcccgaac tcgttttccc
     4261 tgccgacttt accttgcgcg tcgccctgaa aaaagaggaa aaagtcctgc ctgccggcgt
     4321 catccgcgat attttggaag agaaggtagc ggaaatccaa aacaatgaag cccgcaatgt
     4381 cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca gacgacctgc tgccccgagc
     4441 gtttacccgc agcagccgta cagaagcggt gtttaacacc cgccacggct acctgctcgt
     4501 caataacgcg gcttccgcca aagcggaaaa catcctgacc aagctgcgcg aagctttggg
     4561 cggtttggaa gcctcgctgc cgaataccaa acaatcgccc tcttccctga tgaccggctg
     4621 gctgttgcaa gggcattgcg aaggcggttt tgaattagac agcgattgcg aactcaaagg
     4681 tacgggcgat attgttcccg tcgtcaaagt atccaaacaa gacttaaccg ccgacgaagt
     4741 ggttcaacac gtcaaaaacg gtaaaaccgt tacccggctc ggtttggttt ggcgcgaaca
     4801 aatcgccttt atcctcactc aagacttcac actcaagcgc atccaatacc tcgacgtatt
     4861 gcaggaagaa gccgaaagca acggcgacga tgccgccggc cttgccttcg cctcgcaaat
     4921 cctgatggcg gaatccgtca gcaccatgtt ggaagagctg gtttcctatt tgggcggctg
     4981 gcaagattga tttttcagac ggcttttata ccgtcagcca aaatccaatg gaataaccct
     5041 tatgcttaaa catctcgcat tcctactgcc cgccatgatg ttcgccctcc ccgcccagac
     5101 cgccgtccta agcccctatc aggaaaccgg ctgcacctac gaaggcggga tcggaaaaga
     5161 cgggcttcct tcaggcaaag gcatatggcg ttgccgggat gggcgcggtt ataccggttc
     5221 attcaaaaac ggcaaattcg acgggcaagg cgtttatacc gttgccgccg gccgcgaagt
     5281 atttctcgag ccgttcaatt ccgacagtac caaattccgc aatatggcat tgtcgggcac
     5341 gttcaaacaa ggcttggcac acggcaggtt cgccgcctcg caaaacggcg aaaccctctt
     5401 ttattatgaa atgcgaacac ggcatgatta aagaagtgaa actgcccaaa aacaaataac
     5461 cccccgtttt tcaaacagca tcccccaatg ctttatccga acaccgcaaa aaacaaggaa
     5521 accccatgag catcaagtcc gacaaatgga tacgccgaat gagcgaagaa ttcggcatga
     5581 ttgatccctt cgagccgaac caaatcaaag aagccgacgg acagcgcatc atctcctacg
     5641 gtacgtccag ctacggctac gatatccgct gcgccaatga atttaaaatt tttaccaaca
     5701 tcaacagcac catcgtcgat cccaaaaact tcgacccgaa aaatttcgtt accgtcgaag
     5761 acgactgctg catcatcccg cccaattcct tcgcactggc gcgcacggtc gaatatttcc
     5821 gcattccgcg caacgtcctg accgtctgtt tgggcaaatc cacctacgcc cgctgcggca
     5881 tcatcgtcaa cgttaccccg ttcgagccgg aatgggaagg ctacgttacc ctcgagtttt
     5941 ccaacaccac ccccctgccc gccaaaatct acgcaggcga aggcgtggcg caagtcctct
     6001 tcttcgagag cgacgaagtg tgtgaaactt catacaaaga ccgcaacggc aaatacatgg
     6061 gacaaaccgg cgtaaccctg cccaaagcct gaaatacaaa atgccgtctg aacccttatc
     6121 gtcgggtttc agacggcatc tacctattta tccgtggtta gaactgaacc gtcccattga
     6181 tgctgatgct gatttcctcc acacccggtg cggctgaatc cgtacccttc atattgacgc
     6241 ttgccgccat cggcatcgcg cgcagcattt ttgcccgaac agccccatcg cccgcaatat
     6301 ggctgccgat ttgcccaaaa ttcaatttga cgattttata accggacgca cccagaacgc
     6361 ccgccagttt ttcggcacgc gccttgaaac gcaaaacggc atccttgctg acctgatcga
     6421 tgacctcgtt tcggcgttcg cgcgacacgc tgaaatcggt atcttcaagc gaagcatccg
     6481 tctgaacatc agcaataaaa cggtttaaag catcaaaatc cctgccctcc gccttaaatt
     6541 ccgcacgctc ctcccagcct gtttgaatgc gtctgccgtt ggtatattga tagcgcggca
     6601 tcgcactgcg cgataccaat tcggttttaa agctaccatt tttcgatttt ctggtgaaat
     6661 tgttgaattt tttaacaaac tcggcattga cggcattttt gtcccgtcct tccgccgcca
     6721 cctggaaacg cgcggacatt gtatcctgag ccacctcgat acccgccgat tcggaaaatt
     6781 cgacaatatt gtaattcaat gcctcagccg ccgccggaaa agataccgcc agcagggaag
     6841 ccgccaaaac agaacgcagc atatccgtat ccttacttga aaaaccgcca atctaacata
     6901 taggtacgct aaattgcgtt agcataagac aacaatttaa atattcgatc ggtctgcctt
     6961 ataataaccg tctttccgat actgaatcat attatgtccg aaccgttgca cgtcctcgtc
     7021 atccccttat ggtatccacg atccgaacag gatgtggacg gaatattttt ccaaaatcaa
     7081 gcacaggccc tccaacgcaa aggcatcaaa accgccatcc tcgcccccat gttccgttat
     7141 ctgcggaaag aaacagcaag catcctgacc ggtccttacg gttttgcccg ataccggcaa
     7201 agcggtttgg acatctacgc atggcgcagc atgtatttct tccccgcttt ccgtttatcg
     7261 acatcgaccg catccgctgg gtgcgcgccg gtttgaaagc cttcaaacac tacatccgcg
     7321 aaaacggact gcccgacctg attcacgccc actgcatgaa ctatgcaggc atacttgccc
     7381 aaaaaatttc cgaaaaatac ggcattccct acgttttaac cgaacacagc agcaccatca
     7441 cgcgcggtct gatccgtcac caccaatggc agccgatgga aaaagcagcg gcgcccgcct
     7501 ccgcacgtct cgccgtcagc cgccatctcg cacacgtcct gcaacacaaa tacggctgcg
     7561 aatggcagta cctccccaac ataccgggtg gaatattcaa gcaaacattt gaataaaaag
     7621 aaaaaaataa caaaccgcat ttcgtgttct gcaccgtctc gcaccttcgc cgcctcaaag
     7681 gacacgatgt cctgctcact gcttttgccc gggcgttggc acaatgcccg caactgcgcc
     7741 tgaacatcgg cggcagcgga caggaagaac agcggctgaa acagcaggcg gcagacttgg
     7801 gcatcgccca tgccgttaca tttttaggcg cactgcaacc cgaagcaatc ttggatttga
     7861 tgaggaacag cgacgccttc atcctcgcca gccgcaccga aaccttcggc gtggtctata
     7921 tcgaagccct ctcgcaaggg ttgcccgtca tcgccataca ttgcggcggc gcggaatcca
     7981 tcgtttcaga cggcaacgga tacctcgttt ccgttgacga tgccgatgtc ctagcctttc
     8041 tcaaaatata tgaacaccat gccgattttt ccgccgaaca gctcagaacc gactgcctcg
     8101 caacattcgg cgaagatgcc gttatcggca gactgattgc cgtattccgt caggcggcgc
     8161 ggaacacggc gcgaaaacgc ccgaaaaata gattaaaatg acgcaaaaca accttcggat
     8221 tcaaatatga cccataccgt ccacctgcac ttggaagaaa ccgacaaccc gacgctgcaa
     8281 cgcctgtgcg gttctttcga cagcaacctc gattcgctcg ccaaagcact cgacatccat
     8341 atcagccgcc gttttgaaca tttcactttc aacggtgcat ttgcacacgc cggcaaacgc
     8401 gcactgctca aactcttgga aacggcgcag acgcgcgacc taaacgacag catcatccgc
     8461 cttgccgccg tcgaaaccca aaccgaagat gccgggcatc aggaaaaaaa tcacgaacac
     8521 gcctattatt tccgaaccaa gcgcggcagc ataggcggca gaacgccacg gcagaacggc
     8581 tatatccgcg ccctgctcga ccacgacatc gtattcggcc tcgggccggc aggtacgggc
     8641 aaaacctatc ttgccgtcgc cgccgccgtc gatgcgatgg aaaaacacca agtcgaacgc
     8701 atcattttag tgcggcctgc cgtcgaagcc ggtgagaaac tgggcttcct gcccggagac
     8761 ctgacccaaa aagtcgatcc ctacctccgt ccgctttatg atgccctcta tgacctgatg
     8821 ggctttgacc gtgtaaccaa gctgattgaa aaaggcctga ttgaaatcgc cccgctcgcc
     8881 tatatgcgcg gcaggacgct caacggcgca tacatcatcc tcgacgaagc gcaaaacacc
     8941 acgcccgaac aaatgaaaat gttcctgacc cgcatcggct tcggcgcgaa agccgtcatt
     9001 accggcgaca ccagccaaat cgacctgccc aaaaacatca aatcgggatt gaaagacgca
     9061 cgtgagaaac tgcacggcgt ggcaggactg tatttccata ccttcaccgg cgaagacgtt
     9121 gtccggcatc ctctggtgca aaaaatcgtc gaagcctacg aatcggcaga acacgactga
     9181 cactccaatg ccgtctgaaa cccgatgcat ggaaaatcgg gcagagaaaa acagagacgg
     9241 aagccaagct ccgtatccgt ttctttatcc gtccaaccga tacatcaatc gaattttctg
     9301 catcggattg cctcatctcc gcaaaacatt gccgaagttt cgcggttgca cgcctgacgc
     9361 actttctgcc gcgccacgcc ttcattccga tcgcttgcct caaacgtctt ctgaaaaggc
     9421 gtaacgctgc atacatacat ggtggaattt gaaccgaaaa ggtcgatttt gacggtattg
     9481 ccgccgcttt ccaatacggc gacacggtgt tccaactgcg ccagacgtgc ttccaaatcg
     9541 ctgattctgt cggcaaatgc aggcatggcg gcacacgcgg tcaaaatgcc caaaatacat
     9601 tttttcatca atttttcctt gttgcttgaa ccctgcccac ccggacatcc tttttccggg
     9661 cggcagggtc agactttatt tgcagggatg aagcccctta caaatcagaa taatacatga
     9721 atcggcactt tatgcttcat cctatgtgtt gaaacttcat tgctccttgt ctgttgaccg
     9781 ttattcccaa tccgttccca caaccttcgg cttgggtttg cgcctgcgac ggcggcgttt
     9841 tttcgcctgt ccttcgtttt tttcgtgccg ggcggcggcc tcgtttttgg tcatttccgt
     9901 ccgctgttcc gccgatgccg tctgaaacgc cgtccaccac tcggcaagtg cgcggtccgc
     9961 attgccggtt tcggcgcgca agagcaggaa atcataggcg gcacggaaac gcgcctgtgc
    10021 aaacagtttg tgcggacgtg cgcctttgcg gttttcaaac tgcggctgga acatccaaat
    10081 ttcacgcatc gtggcggaaa agcgttgcgg cacgccccaa ccgcgttcga cggtttcgcg
    10141 catcgtattg attgcatcgg acagggcggg cgtaggtttc aaaccctgtt gcagattgct
    10201 tttccaatgg cggtccaact ctggccacat cagggccgcc agtacgaaac cgaccgaaac
    10261 cgatttgtcg gcacgcagcc gctcatcggt atttttcagg gcaagcgccg tcatttttcc
    10321 ggcgatgccg tctgaaacgc gcaaagcatt gagcagcgga tggatgtccg gtatgtcaaa
    10381 tccgttcaaa cgtttcagac actcgcgcgc gtgccctgaa aacagcagtt tcatgatttc
    10441 gtcgaacagc cttgccacag gttcgtgctt cagacggcat atcgattcgg caatcggtgc
    10501 ggcggtttct tccgacagct caaagcccaa tttgcccgac aggcggatgg cgcgcaaaat
    10561 cctgacaggg tcttcctgat agcgttcggc ggcattacca atcataacca gcctgcgggc
    10621 ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct tctttttcag gatcgtaata
    10681 caaggcattg caggtaaaat cgcgccgcat cgcatcttct tcgatgctgc cgtaggtgtt
    10741 gtccttcatg attctgccgt gcgcgttctg atgaaccttc gcaccgccgc gaaacgtcgt
    10801 tacttcgata atctccgcac cgttcatcac atggacaatc tgaaaacgcc tgccgatgat
    10861 gcggctgcgg cggaagagtt tgtgcacctg ttcgggcgtg gcatcagttg cgacatcgaa
    10921 atctttgggt tcgatgccga gcagcaggtc tctgaccgca ccgccgacca cataagcctg
    10981 aaaccccgcc cctttcaggc ggcgtatgac gttttcggcg gcaaagctca acatttcggc
    11041 acggatgttg tgtctttcgg caggaatgac cgttttactt tccgcttttt tactactccg
    11101 accggaaggc agcatcttat tcaaccattt tttcagcata aatattctga cccgaagagc
    11161 ggcatctcca cgggcaaccg tgttcagact gcatcaggcg gtacgattcc acaagatgcc
    11221 gaaaaccata agcaaaaggc gtagaaacta cgccgtccga atcacgccgc cctcgcggcg
    11281 gcaacgcgtc attataacag attgcccctt aaaaaatcag accctgcttt tgtggcggag
    11341 tctgaaattt gatgcacctt atatattgcg tccctaagaa gggatgatta acaaaaatta
    11401 acacccttta ctttcttttc cgattgggaa cattcccgtc gtacaaacaa gcaaatgaag
    11461 gaacagaaat cacattcaaa cggcgttccg ccgcctctac gccgcttccc agtagtcgat
    11521 ataaagctgc aattcgaggt tgtcgcgcca ttcgttggca acggggcggt acaccgtgcg
    11581 gatgtattcg ggaatgtctt cgctgcaacg ccagaacatc gcttcaaatt cgcagccgtc
    11641 tttttgcaac cacgcttttt tgtgtttgcc ttccgcgccc aaaggctgct ggcgaacgac
    11701 gtggaactcg tcggtaaagc tcggcggcgc aaagccctgc ccccaaacgt ggcaggcgag
    11761 gttttgcgcc tgttccaacg tgatgtcgca ggcgggcagg ctgccgtcag taatataggt
    11821 ttgcgacaaa tcgtcttcgc acaccatctc acgcacggct tcttcaaagg ccgtctgaaa
    11881 cgcgggaata ttgtgttcaa gtatgcttaa acccgccgcc atcgcgtgtc cgccgaattt
    11941 caaaatcaaa tcgggatggc gtttggacac caagtccaaa gcatcgcgca agtgcagatt
    12001 gggaatggag cgtcccgaac cgcgcacttc gccgttgtcc gccggcgcaa acacgatggt
    12061 cggacggtaa aaacggtctt tgaggcggct ggcaacaatg ccgaccacgc cttgatggaa
    12121 gtcgtcgcga tacgccacca aagtcgtctg acctgaaggc agggtttcgg gaaaggcgtt
    12181 caacgcgtct cgcagcatag actgctcgat ttcgcggcgt tcgatattga ggttgtttaa
    12241 ccgagccgcc agttcctgcg cttcggaatc atctcgcgcc aacaggcagg cgatgccgac
    12301 cgacatatcg tccagccgtc cggcggcgtt gatgcgcggg cccaacgcaa agcccatatc
    12361 aaacggctga gccttgcgcc aatcgcgccg cgccacttca aacaaggcgc ggatgccggg
    12421 gcgcattttg cccgaacgca tccgtttcaa accttgcgac acgaggatgc ggttgttgtg
    12481 gtcgaggggg acgacatcgg cgacggtacc gagtgcgacc aaatccaaaa ggccgcccag
    12541 attcggctct ttgatgccgt ctgaaaaata attgcggcgg cgcagttcgg cacgcaatgc
    12601 cgtcaacaca taaaaaatca cgcccacgcc cgccaagctt ttgcttggaa aaccgcagcc
    12661 tttttggttc ggattgacga tgatgcagtc gggtacggta tcggctggca aatggtggtc
    12721 ggtaacgatg acatccaaac ccagagcctg cgcgcgcgcc acgcctgcaa tactggcaat
    12781 gccgttatcc accgtaatca gcaaatccac gccttgcgcg gcggcgattt cggcgagttc
    12841 gggcgttaag ccgtagccgt gctcaaagcg gttgggcacg aggaaatcca ctttcgcgcc
    12901 catcgccgcc aaaccgtcca aaccgacggc acacgcggtc gcgccgtcgg catcatagtc
    12961 ggcgacaatc aggatttttt ccttgcgttc aaccgcatcc gccaaacggc cggcggcggc
    13021 ttcgcaattc gtcagcgatt gataaggcag gagggaagcg agtttgtcgt ccaactcggc
    13081 aggactttgc acaccgcgcg aggcacaaag ccgggcgatt aaaggatcgg cgccggcggc
    13141 gagcaaatga ttgaaaacgt cggtattgac ggggcgggtt tggattttga ctgacatagc
    13201 gcggggtttt acagtgcggg tgtgcttgaa atgccgtctg aaggcttttc agacggcatt
    13261 gccgttcatt tgtctgagga attcgcggct gcaatcagag ccataggcaa gaccagcacc
    13321 gcggtcgccg catccaatat caacaagggg ggcgtatact aagatatttc caaacagctt
    13381 ggatttgtcg gtatgttttt cagtaaccgt ataataaata tcggcgggca cactttgctc
    13441 aaaatgataa tcggcgttca gtttttgcgg tgtggcgtag tatttgcctt tggcggatac
    13501 gcagcgcgtg taaatggtcc gattgtcgag tttgaccgct ttaaactcaa gctgtttcag
    13561 cttggcgatg tcgtcaggtc tgtcggtatc atagcgcagg caaagacctc cggtactgaa
    13621 attctggctg ccgggcgctt cgaatttgac cggcagggct tggtggcggg catagctcgg
    13681 ggtatcctca actatttgga agggcttgtc caacccggcc ttcaaaaggc ccgtcagctt
    13741 cgccgaatct tcgggattga cggcgaacca gtatttcccg cccatcatca ccaggctgcc
    13801 cttttccaat tgggcattgt cttcggcaac cacaccgaag gcgcggattt ggtctttgtc
    13861 aacgtgtttg cgggtgattg tttggctgac cgggttgttc atcccccgca acatcatcgt
    13921 acagccgttc agtatcaagg ttgccgccaa aacggcggcg gtcgttttac ggaacaacat
    13981 tttatcttcc aatctaaata acggcggatg ccgtctgaaa atatgcctaa atttccgcgc
    14041 ccccgttttc catcacggtt ttcaattctt gaaacagggc gcggaagttt ttcggcggtt
    14101 tgtcctgttc ctgttctttt ttggtgttgc ggacgagcgt cctcagcctg cccgcgccgg
    14161 cgtttggaaa atcggacata aactgcgtca gcgcgccctc gtctgccaac aaccgtacac
    14221 gcgcctgttc cacgcgttgc aaaaaggcgt tgtgcgccgc atcgtcgccg cgcagcttgg
    14281 caaggaacgc ctcgatgggc gcgggatcgg tatcgcgcat cagacgtccg ataaattgcg
    14341 cctgacgttt gagcgcgccg ttggacgtaa tttttttata ggtaacgacg gcctcgtaca
    14401 aatcttcatc caaaccgatt tttttcagcg tgtcgtttga gagcctggtc agttccatac
    14461 ccaaatcctg caaaccgttc atctgctttt tcatttgggt tttgctgatc cactcgtctt
    14521 cttgttcaaa catcttgatt ctcgaatttt ttccaatagc gtattttaac agaatgccgc
    14581 cgcgttcaga cggctttttg caccgcacgc cctgcaagcg gcacgctttt gcccctgccg
    14641 cccgaaacgg ttaaaatgcc gcaacgctcc accaagaaag attgttatgc tgttcaacca
    14701 caccgcttcc gaactcctcg acctctgccg ccgcacgctc gacttggcaa aagcagcggg
    14761 cgcgaccgcc gccgaagccg acttcagcga atcgctcgga caaagcgtca gcgtgcgctt
    14821 gggcgaaatc gaacaaatcg agttccagca ggacaaatcg ctggacatta ccgtttacgt
    14881 cggcaaacgc aaaggccgcg ccggcaccgc cgacttctcc gaacaagccc tgcgcgacac
    14941 cgtccgcgcc gccgccgaca tcgcccgcca caccgccgaa gacggttgcg ccggacttgc
    15001 cgaccccgag ttgatggcgc aacacatcgg cgaccccgac ctttaccacg aatgggattt
    15061 ggatacggaa gccgccgtcg gcttggcaaa acaatgcgaa caggcagcgc tggacacgga
    15121 cagccgcatc gaaaactccg aaggcgcggc ggtgcaaacc ggacactacc aatacgttta
    15181 cggcaacacc cacggttttg ccgcacaccg gcaaagcacg caccacagca tttcctgcag
    15241 cgtcgttgcc gccgacgaaa acggaatgca gcgcgactac tggtacgatt ccgcctgccg
    15301 ccatccggat atggacagcc ccgaaaccat cggtcaaacc gccgcccgcc gcactttgcg
    15361 ccgactcggc agccgcagca tcccgacagg cagctacccc gtcctcttcg ataccaccgt
    15421 ttcgggcggg ctgatcggac acctcgtcgg cacactctcc ggcggcgcgc tctaccgcca
    15481 aagcagtttc ctgatcgaca gcatcggcaa aaaagtcctg cccgatttcc tcaacctgcg
    15541 cgaagaaccg cacatccccc gctctttccg cagcagctat tttgatgcgg aaggtgttgc
    15601 caccgcgccg cgcttcgtga ttcaaaacgg cattgtcgaa ggctatttcc tcggcagtta
    15661 cagcgcgcgc aaactcggaa tgcagaccac gggcaacgcc ggcggcgcgc acaacctgta
    15721 tttgaaccat acgcacgaaa cgcaatccga cctgctgaaa gaaatgggca cgggattgct
    15781 ggtaaccgaa ttgatgggac agggtgcgaa caccatcacc ggcgactatt cgcgcggcgc
    15841 ggcgggtttt tgggtggaaa acggcgtgat tgcctacccc gtccacgaga ttaccgttgc
    15901 cggacgcttg caggatatgt accgcgacat cgtcggcgtg gcggatgacg ctttgcggcg
    15961 ttcgtccaac aaaatcggtt cgattctgat tgcggggatg acggttgccg gaagctgaac
    16021 gccctgccga tgccgtctga agacgaaggc ggcgcgtttt gccccgtgcc tgcccgtttg
    16081 ccgttttatg ccgcaccgcc ttttaccggc gacattgcac atttttttca tcagcactga
    16141 ttttccctta tccgtcaaaa tactaagcca cttgtccaca acagggtaca cacaagttca
    16201 ggataaggcg ggcggggatt ggggactttt ccgccatcgg caaaacggca gacaaaaacg
    16261 gcgggacatt gcctgtcccg ccgttttcaa atatacattt gaccgtacat caggaattaa
    16321 gctgcctgaa tgttggcggc ctgtttgcct ttagggccgg tggttacgtc gaaagagacg
    16381 cgttggcctt ctttcagggt tttgaaacct tccatattga tcgctgagaa gtgtgcgaac
    16441 aaatcttcgc cgccttcgtc aggagtgatg aaaccaaaac ctttagcgtc gttaaaccat
    16501 tttacgatac cggttgccat tagaaacttc ctatacttaa aaattaacaa aatcagcaaa
    16561 ataaggcata cagcaaactt aaacgttcag cgtttccacc cagctttata tacttggtcg
    16621 agtgccgact tttgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt
    16681 tttcaaaaaa gtgggaaaat aggcgaaagt accgaaaata caacaaaaat gccgtctgaa
    16741 cgcggctttc aaatgggaca cgctatgacc gcgcaacacc aatccgacac gcttttgcac
    16801 cgcctgaaca ccctgccgcc gaaacgttac ggcgtatttt tattgaacga cgattacacc
    16861 acgatggaat ttgtcgttga aatcctgacc gaaatcttta tgctcgggca ggaacaggcg
    16921 gtagcggtaa tgctctcggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc
    16981 gatatcgccc aaaccaaaca gcaacaagtc atgcagcgcg caaaagccga aggacatccg
    17041 ctgcaatgta ttgtcgagga gatttaatat gcttgcccct gaattggaac agattttgca
    17101 gcagctttac cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct
    17161 tttggtactc atcgaagaag atgccgccgt gccgaacgtc ttaaaactct gcggtgcgga
    17221 tttaaaagcg gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc
    17281 cgaccacctt ttagacacgg tcgaaacccg gcccacgctc ggcttccaac gcgtcatcca
    17341 gcgggcgatg gtgcataccc aatcggcagg caaaggcttg gccgagcctt tggacgtttt
    17401 ggtcgcgctg atgagcgaaa ccgacagcca cgccgtctat ttcctcaagc tgcaatcggt
    17461 tacgcgtttt gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagacga
    17521 tggcaactat ttttcagacg gcatggacga cgataatgag aaccgcacca aaccgggcaa
    17581 aaacccttta tcggcgtaca ccgtcaacct caacgccgaa gtcaaagccg gccgtatcga
    17641 tcctttgatt ggtcgcaaac acgaaatgga acggctggtg caaatcctgt gccgccgccg
    17701 caaaaacaat ccgcttttgg tcggcgaagc aggcgtgggc aaaaccgcgc tggcggacgg
    17761 tttggcacat caaatcgtca acgacgacat tcccgacgcg ctcaaagagg ccgaagtgta
    17821 cgcgctggat atgggttcgc ttttggcggg cacaaaatac cgcggcgact ttgaagcgcg
    17881 ggtcaaatcc gtcttgaaac agctcgaaaa aatcccgcac gccattttat tcatcgacga
    17941 aatccacacc atcatcggcg cgggcagcac cggcggcggc acgatggacg cgtccaacct
    18001 gctcaaaccc gcattggcga aaggttcatt gcgctgtatc ggcgcaacca cttacgacga
    18061 ataccgtact atttttgaca aagaccacgc cctaagccgc cgcttccaga aaatcgacgt
    18121 ggtcgaaccc accgttgccg aaaccgttca aatcctgcgc ggcttgaaac cgatgttcga
    18181 agccttccac caagtccgct acacgcaagg cgcactcgaa gccgccgccg aactctccgc
    18241 ccgctacatc aacgagcgct tcctgcccga caaagccatc gacgtaatgg acgaagcagg
    18301 cgcggcgcaa cggattctgc ccaaatccaa acagaaaaaa gtcatcggca aagcgcaaat
    18361 cgaaaccgtc atcgccaaag tcgcgcggat tcccgaaaag accgtgtcgc acgacgacaa
    18421 acaagtgttg caattcctcg gccgcgattt gaaaaacatg gtttacggtc aggaaaatgc
    18481 catcgatgcg ttggttgccg ccgtcaaaat gtcgcgctcc ggccttgccc tgcccgacaa
    18541 gccgataggc agcttcctct tctccggccc taccggcgtc ggcaaaaccg aagccgccaa
    18601 acagcttgcc tactcaatgg gcgtaccgct gcaacgcttt gatatgtccg aatacatgga
    18661 acgccacgcc gtatcgcgac tcatcggcgc gccaccgggc tacgtcggct ttgaacaagg
    18721 cggccttttg accgaagccg tcaacaagca gcctcattgc gtgttgctct tggacgaaat
    18781 cgaaaaagcc catccagata ttttcaacgt cctcctgcaa gtcatggacg caggcaaact
    18841 gaccgataac aacggtaaga gcgccgattt ccgcaacgtc atcctgatca tgaccaccaa
    18901 cgcaggcgca gaaagcctca gccgacccag cctaggcttt accgccaaac gcgagcgcgg
    18961 cgacgaaatg caggcaatca acaaactctt cacgcccgag ttccgcaacc gcttggacgc
    19021 gatcatcccg tttgcacctt tgtccgaacc catcatcgtc aaagtcgtgg acaaattcct
    19081 gctccagctc gaacaccggc tcctcgacaa aaaagtcgaa gccgaattca caccggcatt
    19141 gcgtaaatac ttggcagaaa aaggtttcga cccgcaaatg ggcgcgcgcc cgatgcaccg
    19201 cctgattcag gaaaaaatcc gcaaaccgct cgccgacgaa ctcctgttcg gcaaactcgc
    19261 cgacggcggc tttgtgcgga tagactggga tgcggcgaaa gaagaagccg tattgaagtt
    19321 taagaaaagc aaggtcaaaa taaaaaccgc ctccgcataa aatcaaaaat gccgtctgaa
    19381 aggttcagac ggcattttgt caaatatcgg cccctattcc ggcaaacttc tccacaaacg
    19441 ccgcgatttt ttggaagacg ctttgtttct tcgtcagata ttgcgggttg agcggactca
    19501 ttttcggcag ggtttcggtc agttccgtgc cgttttcgct ggcatagccg cgtttgagcg
    19561 agccggtcaa atagcgtttg gcggcggttt cgttcaggcc ttcggcggca atcaatcctg
    19621 ccgcttcgtg ccgcatcacc tcttgcgcgt aggtgtagaa ggtttccagt atggcgggaa
    19681 cgtcgggcac tttgtccaaa tccgtatcgt tgatgaaatc cacaatcagg ccctctttgg
    19741 cgcggtggcc gatgctggcg cggatgatgc ggcggatttc ttccaccagc tccgctttgc
    19801 ctttgatttt tttgtgatgc tcgaaaacca gttgcaggat gtaatccaga ttgatttcct
    19861 gcgatttgag caaatccacc tcaaaaacca catcgtccca gtcgattttt gactgttctt
    19921 tctgttcgcc tgctttttgg cggcgcagcc agtctcggat gtcgttatag gcggaacggt
    19981 agtcctgcac cgccctttca gacggcatcg gcactttccg catttcctgc acgtcttcgt
    20041 cgctcaggta gtatttttcc tgaaacgcct tcatcgcatc ttcgtccgcc gcgtccacat
    20101 tctgcaattc gcgcagcgcg gcaaattcat cgtagttctg caatacattt tccgcccgca
    20161 ggtattcgcc gaagagtttg gcaaaatctt ttttgtcttt ttccgtttcg attttgtcgg
    20221 gatcggggaa acgctcgtgc aattcttttg ctacatccag ataaccgcgc cgtgcttcgc
    20281 cggtctggct gtcgatatag ccgttcatgt attcttcgta gcttttttcc agcactacat
    20341 ttttggtgtt tttgtcgccg aagagcgtga tcgcatcaat ggttgcctgc tccaagtcgc
    20401 ggaagcagac aatgttgccg aaggttttgg tagcatcgta aatgcggttg gtgcgagaaa
    20461 acgcctgcat caggccgtga tagcgcaggt ttttatcgac gaacagcgta ttcagcgtcg
    20521 gtgcgtcaaa acccgtcaaa aacatgccga ccacaatcag caagtccact tcccggtttt
    20581 tcacccgttt tgccaaatcg cggtagtagt tctgaaaggc tttgctgtcc gtgccgaaat
    20641 tggttttgaa acaggcgttg taatcgttga tgacagcctg caaaaattct ttcgcgctgc
    20701 tgtccatcgc ttccggttca aagttctcat cgacaatttc accgacggcg ttttgctctt
    20761 cattggccgc aaaggaaaaa atggtggctg ttttcaacgg gtgcgggctg tctgcctgtt
    20821 gtgttttgaa cgcttcgtaa tagcacttcg ccgcatccac gctgctgacg gcaaacatgg
    20881 cgttgaagcc tttgccgccc gcattcagcc ggtgcgtttt ctgcctgaac cggttcagga
    20941 tatattgcgt gatttcgcgg atgcgttcgg ggtgcagcag ggcttggcgg ttttcggcgg
    21001 cactcagttc cttctcgtcc tgttccgctt ccacggcttt gaactgcggg cgcacatcgt
    21061 tgtaatccac tttgaatttc aatacttttt catcgcggat ggcatcggta atcacataag
    21121 aatgcagctc ccgcccgaac acgcccgccg tggtttccgc gcccaaagcg ttttcgggaa
    21181 aaatcggcgt gccggtaaag ccgaactggc agaatttttt aaatttcttt ttcaggtttt
    21241 tttgcgcttc gccgaattgc gagcggtggc attcgtcgaa aataaataca acctgccggt
    21301 gataaaccgg cagattatct tcacttttca tcaggttgtt cagcttttgg atggtggtaa
    21361 cgatgatttt gttgccgtct ttttccaaat tgcgtttcaa gcccgccgta ctttccgagc
    21421 cgtttacgct gtcgggcgaa aaacgttggt attccttcat cgtctgatag tccaaatcct
    21481 tcctgtccac cacgaagaaa accttgtcga taaacgccga ttccgtcgcc agacgcgccg
    21541 ccttaaagcc ggtcagcgtt ttgccgctgc ccgtggtatg ccaaacatag ccgccgcttt
    21601 ccggcccgct ccaattcttc gcctgcgccg agctgttgat tttccacaaa atgcgttcgg
    21661 cggcggcaat ctggtacggc cgcataatca gcagcgtatc attcgcatcg aacacgctgt
    21721 aatgcagcaa aacgctcagc aatacgcttt tctgcaggaa cgtggcggta aagtctttca
    21781 aatccttaat cggatgattg tccgaccgcg cccaattcat cgtgaaatcg aagctgtttt
    21841 tgtcgcgctt ggtggtgttg gcgaaatagc gcgtgtccgt gccgttggaa atcacgaaga
    21901 tttgcaggaa tttgaacagc gaattttcgc tgttgaagct ctctttgctg taacggtgca
    21961 cctgattgaa tgcctcgcgc accgccacac cgcgcttttt caattcaatc tgcaccagcg
    22021 gcaggccgtt taccaacacg gtaacgtcat aacggtttgc atgcgtgccc gtctgctcaa
    22081 actggttgat aagctgcaca tggttgcggg caaggttttt cttgtccagc agataaatgt
    22141 ttttcagacg gccgtcatca aaagcgaaat cgtaaatatg gtcgtcgtgg attttgcggg
    22201 ttttatcggt aatgttttca gccggcctgt ccagatattc cgtcaaaaac cgcgcccatt
    22261 cgccgtctga aaacgccaca tcgttcagcc gctgcaactg ggcgcgcagg ttttccagca
    22321 gcctgctttg gctgttcaaa tccttgcggt attcgtaccc ctgattttgc aaatcggcga
    22381 ttaactccgc ttccaaccgg ttttccgatt ggtagctgcc cgactgttcg attttttcat
    22441 attggtcgag cacgatgaaa ttcggcgttt cggcgatggg tttggtttcg aggttcatgg
    22501 gtcgtccttt gcggtgtcgg gttttatagt gaattaaatt taaaccggta ctgcgttggc
    22561 tcgccttagc tcaaagagaa cgattcccta aggcgcccaa gcaccaggcg aatcggttcc
    22621 gtactatttg tactgtctgc ggctcgccgc cttgtcctga tttttgttaa ttcactataa
    22681 acggtttgat gttcggattg ggctgcctga aaaagtgcag gctgcttttt caggcagcct
    22741 tggggaaggc aagcagctgt tcgcggtaat attcgtattg tttgcggcgc agggcgattt
    22801 cgtgcggcag gccttcgctg acggagtggg tcagggtatc gaatttgcct aggatggcga
    22861 cgattttttc ctgttcgggg agcgggggga tggggatttt atattgcata atcgctgctt
    22921 tgctgccacg cggcattttt gcacctttgg cgtgcttcat attaaaagca aaaaatttat
    22981 catcggccaa tacctgatac agatattttg gattaacctt ttcatctgtt acccggataa
    23041 ccaaaacatc accgtttgtt ccgcccgtgc agtctgcctg ccagattttt ttcaaatacg
    23101 gacgaatatt tccaatcaaa atgtcattga caatatattc tgtcattttt ccttcgcttg
    23161 gaacataacc ggataacttt ttaccttctc tgttttgtaa gagattatcc acgccaacgt
    23221 aattatgttc gttcagttta tccgaacaaa tacggttttt tgaatattcg gcaacctccc
    23281 ccaacgtctt ccaaaccaca tttttcagac ggcattgata gccatcagct atcccccccc
    23341 gatttgattg tcaaaatcta aaagtaag
//
LOCUS       DAXFHF010000031        23161 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXFHF010000031 DAXFHF010000000
VERSION     DAXFHF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23161
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.031"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(63..401)
                     /locus_tag="ACFIRM_001115"
     CDS             complement(63..401)
                     /locus_tag="ACFIRM_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC3028758.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            552..4508
                     /gene="purL"
                     /locus_tag="ACFIRM_001116"
     CDS             552..4508
                     /gene="purL"
                     /locus_tag="ACFIRM_001116"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC3028759.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4579..5790)
                     /locus_tag="ACFIRM_001117"
     CDS             complement(4579..5790)
                     /locus_tag="ACFIRM_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC3028760.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARTQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5864..6175)
                     /locus_tag="ACFIRM_001118"
     CDS             complement(5864..6175)
                     /locus_tag="ACFIRM_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3028761.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6407..7159
                     /gene="gloB"
                     /locus_tag="ACFIRM_001119"
     CDS             6407..7159
                     /gene="gloB"
                     /locus_tag="ACFIRM_001119"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC3028762.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7393..8847
                     /gene="mgtE"
                     /locus_tag="ACFIRM_001120"
     CDS             7393..8847
                     /gene="mgtE"
                     /locus_tag="ACFIRM_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC3028763.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8933..9841)
                     /gene="hslO"
                     /locus_tag="ACFIRM_001121"
     CDS             complement(8933..9841)
                     /gene="hslO"
                     /locus_tag="ACFIRM_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC3028764.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AISCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10086..10754
                     /locus_tag="ACFIRM_001122"
     CDS             10086..10754
                     /locus_tag="ACFIRM_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3028765.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLL
                     GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGAPVARSELQPGDMV
                     FFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARRIKKNDPS
                     RFLN"
     gene            10773..10991
                     /locus_tag="ACFIRM_001123"
     CDS             10773..10991
                     /locus_tag="ACFIRM_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028766.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11136..11480
                     /locus_tag="ACFIRM_001124"
     CDS             11136..11480
                     /locus_tag="ACFIRM_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC3028767.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11480..12172
                     /locus_tag="ACFIRM_001125"
     CDS             11480..12172
                     /locus_tag="ACFIRM_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC3028768.1"
                     /translation="MSEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGMGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12239..13459
                     /gene="argJ"
                     /locus_tag="ACFIRM_001126"
     CDS             12239..13459
                     /gene="argJ"
                     /locus_tag="ACFIRM_001126"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC3028769.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVMPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13519..14928
                     /locus_tag="ACFIRM_001127"
     CDS             13519..14928
                     /locus_tag="ACFIRM_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC3028770.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            15116..16507
                     /gene="hrpA"
                     /locus_tag="ACFIRM_001128"
     CDS             15116..16507
                     /gene="hrpA"
                     /locus_tag="ACFIRM_001128"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC3028771.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDDTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            16575..18077
                     /locus_tag="ACFIRM_001129"
     CDS             16575..18077
                     /locus_tag="ACFIRM_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFC3028772.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDGLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEPNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENSQLLKLIDKHSIASTQY
                     FPIYGFSRICSDIHKEAVLKRQQKEKLDHFIEEINRRCKNNHSSIQSILDDENISDTY
                     KNDAIAWGIWNNQLSEDEVENYLKNFVNKKNTHYKRLLCMFDYKKYADTV"
     gene            18304..19881
                     /gene="lpt3"
                     /locus_tag="ACFIRM_001130"
     CDS             18304..19881
                     /gene="lpt3"
                     /locus_tag="ACFIRM_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC3028773.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKASRLLIAVFFAFSMIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            19947..>23161
                     /locus_tag="ACFIRM_001131"
     CDS             19947..>23161
                     /locus_tag="ACFIRM_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC3028774.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     SFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         5658 a         6763 c         5754 g         4986 t
ORIGIN      
        1 gtacagaccc aaaaacaaat gccgtctgaa agcctttcag acggcatttc cattccaaac
       61 gctcaaaccg ccgcgtccga acgttcgccc gtgcggatac ggattgcctc ctcgaccggc
      121 agcacaaaaa tcttgccgtc gccgattttg cccgaacgcg ccacctcgac aatcacgtca
      181 atcgcgcgtt ccacggcatc atccgccaac accaactcga ttttgacctt gggcaggaaa
      241 tcgacggcgt attccgcacc gcgatagatt tccgtatgcc ccttctgcct gccgaaccct
      301 ttgacctcgc tgacggtcat gcccgtaatg ccgatttccg tcaacgcctc gcgcacgtcg
      361 tcgagcttga acggtttgac aatcgcctcg atttttttca taaaatttcc tttgaacaaa
      421 caatacaaac acatccgaaa aacgggaacc cccgtcagat tgtcaacatt ttaaaccaaa
      481 atacccaagc aatacagccc ccgttgcgca tataatgaca gcttttccaa ccgcatttga
      541 gagccgaatc catgtctgtt gttttgccct tgcgcggcgt taccgccctt tccgatttcc
      601 gtgtcgaaaa actcttgcaa aaagccgccg cactcggcct gcccgaagtc aaattaagca
      661 gcgaattttg gtatttcgcc ggcagcgaga aagcacttga tgccgcgact gtcgaaaaac
      721 tgcaagcctt gttggcggcg caaagcgttg aacaaacgcc cgaagcgcgc gagggtttgc
      781 atttgttttt ggtaacgccc cgtttgggta cgatttcgcc gtgggcttcc aaggcgacca
      841 atatcgcgga aaactgcggt ttggcaggca tcgaacgcat cgagcgcggt atggcggtgt
      901 ggctggaagg tgcgcttacc gatgaacaga aacagcaatg ggcggcgttg ctgcacgacc
      961 gcatgaccga atccgtgctg cccgattttc agacggcatc caaattattc caccatctca
     1021 aatccgaaac cttctccacc gtcgatgttt tgggcggcgg taaagaggct ttggtcaaag
     1081 ccaataccga aacgggtttg gcactctccg ccgacgaaat cgattatctg gttgaaaact
     1141 atcaggcttt gcagcgcaat ccgtccgatg tggagctgat gatgttcgcg caggcaaaca
     1201 gcgaacactg ccgccacaaa atcttcaacg ccgatttcat cctcaacggc gaaaaacaac
     1261 cgaaatccct gttcggcatg atacgcgaca cgcacaacgc gcatcccgaa ggtacggtcg
     1321 tcgcctataa agacaattcg tccgtgatca aaggcgcgaa aatcgagcgt ttctatccga
     1381 atgcagcgga aaaccaaggc taccgtttcc acgaggaaga cacccacatc atcatgaaag
     1441 tggaaacgca caaccacccg accgccatcg cgccgtttgc gggcgcggca acaggcgcgg
     1501 gcggcgaaat ccgcgacgaa ggtgcgacgg gcaaaggttc gcgtccgaaa gcgggcttga
     1561 ccggctttac cgtgtccaac ctcaacatcc ccgacctcaa acagccgtgg gaacaagact
     1621 acggcaagcc ggaacacatt tcctcgccgt tggacatcat gattgaaggc ccgattggcg
     1681 gtgcggcgtt caacaacgaa ttcggccgcc cgaacctctt gggctacttc cgcacctttg
     1741 aagaaaaatt tgacggtcag gttcgcggct accacaaacc gattatgatt gccggcggtt
     1801 tgggcagcat tcaggcgcag cagacgcaca aagacgaaat ccccgaaggc gcactgctga
     1861 tccaactggg cggcccgggc atgctcatcg gcttgggcgg cggtgcggct tcttcgatgg
     1921 ataccggtac aaatgatgcg tctttggact tcaactccgt acaacgcggc aaccctgaaa
     1981 tcgaacgccg cgcgcaggaa gtcatcgacc gctgctggca gctcggcgac caaaacccga
     2041 ttatctccat ccacgacgtg ggcgcgggcg gcttgtccaa cgctttccct gaattggtca
     2101 acgatgccgg acgcggcgcg gtattcgagc tgcgcgaagt gccgttggaa gaacacggtt
     2161 taacgccttt gcaaatctgg tgtaacgaat cgcaagagcg ttatgtcttg tcgattttgg
     2221 aaaaagattt ggacaccttc cgcgccatct gcgaacgcga acgctgcccg tttgccgtag
     2281 tcggcacggc gaccgacgac ggtcatttga aagtgcgcga cgatttgttc tccaacaacc
     2341 ccgtcgattt gccgctgaac gtcttgctcg gcaaaccgcc caaaaccacg cgtaccgaca
     2401 aaacggttac gccgtccaaa aaaccgtttc acgcgggcga tatcgacatc accgaagccg
     2461 cctaccgcgt tttgcgcctg cctgccgtag ccgccaaaaa cttcctgatt accatcggcg
     2521 accgcagcgt cggcggcatg acccaccgcg accaaatggt cggcaaatac caaacccccg
     2581 tagccgactg cgccgttacc atgatgggct tcaacaccta tcgcggcgaa gcgatgtcta
     2641 tgggcgaaaa accggccgtc gccctgtttg acgcgcccgc ctcgggcaga atgtgcgtcg
     2701 gcgaagccat caccaacatc gcggcagtca acatcggcga catcggcaac atcaaacttt
     2761 ccgccaactg gatggcggcg tgcggcaacg aaggcgaaga cgaaaaactc taccgcaccg
     2821 tcgaagccgt ttctaaagcc tgccaggcat tggatttgag cattcccgta ggtaaagaca
     2881 gcctgtcgat gaaaaccgtg tggcaggacg gcgaagagaa aaaatccgtg gtttcgccgt
     2941 tgagcctgat tatttccgcg ttcgcgcctg ttaaagacgt gcgaaaaacc gttacgcccg
     3001 aattgaaaaa cgtcgaaggc agcgtattgc tgtttatcga tttgggcttc ggcaaagcgc
     3061 gcatgggcgg ctcggcgttc ggtcaggtgt acaacaacat gagcggcgac gcgcccgatt
     3121 tggacgatgc aggccgtctg aaagcgtttt acagcgtaat tcaacagctt gtcgccgaag
     3181 acaaactttt ggcgtatcac gaccgcagcg acggcggttt gtttgcgacg ctggcggaaa
     3241 tggcgtttgc ggcacggtgc ggtattagtg ccgatataga ttgcctgatg gataaattcc
     3301 tgccgattca tctcccggat tttcaaggcg accctgccga agacttatct gacgaacttt
     3361 ataatcatgc cgccattaaa atcttattca atgaagaatt aggcgcggtt atccaaatcc
     3421 gccaaaaaga tagggattat gttgatgcgg catttgaaac ggccggctta accgatgcgg
     3481 tcagccggat tggctctcct gattttgaca atgagtttat ttctttcttt ggttacggtt
     3541 atttcttgga acaaaaccgc gccgacctgc aacgcgcgtg gcaggaaacc agccacgcca
     3601 tccaacgcct gcgcgacaac cctgcctgcg ccgacagcga gttcgccctg attggcgaca
     3661 acgaacgcag cgcattgttt gccgacgtga agttcgacgt gaacgaagac atcgccgcgc
     3721 cgtttatcaa cagtggcgcg aaacctaaaa tcgccatcct gcgcgaacag ggcgtaaacg
     3781 ggcaaatcga aatggccgcc gccttcaccc gcgccggatt cgatgcctac gacgtgcata
     3841 tgtccgacct gatggctggc cgcttccgcc ttgccgactt caaaatgctg gcggcgtgcg
     3901 gcggcttcag ctacggcgac gtactcggcg cgggtgaagg ctgggcgaaa tccatcctgt
     3961 tccaccctgc cttgcgcgac caattcgccg ccttctttgc cgatccgaac acgctgacat
     4021 tgggcgtgtg caacggctgc caaatggtca gcaaccttgc cgaaattatc ccgggcgcag
     4081 aaacatggcc gaagttcaaa cgcaacctga gcgagcagtt tgaagcgcgc ctgaatatgg
     4141 ttcacgtccc caaatcagcg tcgctgattt tgaacgaaat gcaagactcc agcctgcctg
     4201 tcgtggtcag ccacggcgaa ggccgcgccg acttcgcgct tcacggcggc aatatttccg
     4261 ccgatttggg catcgcgctg caatatgtgg acggtcaaaa ccaggttacc caaacctatc
     4321 cgctcaaccc caacggctcg ccgcagggca tcgccggcat taccaacgcc gacggccgcg
     4381 ttaccattat gatgccccac cccgaacgcg tgtaccgtgc cgcacaaatg agccggaaac
     4441 cggaaggctg gacggaactg tccggctggt accgcctctt tgccggcgca cgtaaagcct
     4501 tgggctaaac cgtctgccca aacaaatgcc gtctgaaaaa ggttttgaaa cccgtttcag
     4561 acggcatttt gaccattctc aagccctgcc ggtactcaat cccgctttac cgttttcccg
     4621 caaagttttc agcaatactt tccacgccga caatttaggg ttggactgtt ttccttgtcc
     4681 gatcaaatcc atttgcgtca tataccagtt catttctaac attgcacccg cttttttatc
     4741 aacaaatgct acgccagttt ggatgcggtc tgcttgtacg gtttggtaag ttctgttttg
     4801 cattttgttc gccaaatcag ggactaaggc aaacgggcgg gctatgccga ccaaatccaa
     4861 atgaccgctg gataaggcat cttccatggc ggtttgcgaa cggaatccgc cggtgataat
     4921 cagcggggct tggctggctg cacgggcttt ttcggcgtaa tcgatgaaga aggcttcgcg
     4981 tttgcgggtg ctgtctttgg cggcgagcat ttgcgggctt tcgtagttgc caccggaaac
     5041 ttcaataaaa tcaatgccca tttccgacag tttttgcacc acctgcaccg attcgctttc
     5101 gtcaaatccg cctttttgga aatctgccga attgagtttt acgcccacca agaaatcttt
     5161 gcccgcggca gcgcgaatag cggtgtaggt ttccaaaagg aagcgcatac ggttttccaa
     5221 actgccgccc cattggtctt ggcggcggtt gtggtgcggc gagaggaatt ggctgatgag
     5281 ataaccgtgt acggcgtaaa tttgtacgcc tgaaaagcct gcctgttcgg caattttggc
     5341 agtttgtacg aattgctgaa tcagcccgtt gatttcatcg gcactcaatt cgcgcggcgg
     5401 attgataaag ccattcatgc ccaccagcgg cacggcgctc ggtgcaagcg gtgttttatt
     5461 gaccaccgca ggcgactgtt tgcccgcatg attgatttgc atgatgagca gcgtgtcgtt
     5521 ttgcgtgcgg gctttcgccc attttttcag catttcaagg gcgcggtcgt ctgaaatcaa
     5581 cacatcgttt atcgaacctt tgccgctttc agccaccatc acattgcccg tcaccaaaac
     5641 gcctgcgccg ccttctgccc aagcgccgta caggcggaca agtttttcag acggctggtc
     5701 gttttgggca agttgctctt ccatggcgga tttgaagata cggtttttgg cggttttgcc
     5761 gttttgaaaa gtgaatggcg tgaataacat gatcgttcct ttaagttgat aagtttaaaa
     5821 atgtaaacgt atttgggtaa aaataaaggg ccaacggata accctatttt gaaagcgggc
     5881 aattttgagc catcaaatcg gcaagctgtt tcaatctcgc caccgcatcc gccatggcaa
     5941 tactgtaaaa ccgctctttg ccgacctgct ccaccgccac cgctccggct tgcagcatga
     6001 tttttaaatg gtgcgacaca gcagggcgcg acagatgcag atgctcggtc agctcattca
     6061 cattcatcct accgtgtttc cacaatacat gcaggatctg atggcggttt tcatcgctca
     6121 atacggtaaa aatgggtatg cactcgcgca ttaaattcat ggtttgttgc ggcatattcg
     6181 atctattgag tttatatatt taaaaattcg aacgtatttt agagggaagg tcggcagtta
     6241 tcaaaagcag tttggtttgc agtttggcaa tatattgttg ctgaaaggtt tttataagaa
     6301 aaaatgccgc ctgaaacagc atgatggcgt ttgctagggt tccgctgtat attaacgcct
     6361 ttcggataca cctttcagac ggcatccgaa ccatcaagga acactcatga aaattacccc
     6421 cgtcaaagcc ctgaccgaca actacatctg gatgatacag cacggcaacc atgccgtctg
     6481 cgtcgatcct tccgaaccct cgcccgtctt ggaattcctc gtacgcaacc gactcatgct
     6541 cgcccaaacc tgggtaacgc acccccatcc cgaccacgaa ggcggtgcgg cggcactctg
     6601 gcgcggctac atggaatcgc ccgtttacgg cgaatccgac atcgaagccg ccacccacac
     6661 cgtaaccgcc ggcacacgat tcaccttcgg cgacggtcag gttaccgttt gggcaacccc
     6721 cggccacaca gaccgccaca ccagctacct tctcgaaact tcagacggca tacacgtttt
     6781 ctgcggcgac accctttttt ccgccggctg cggacgcgtg tttaccggca cggtcgaaca
     6841 gctttacgac agcttccaac ggttcaacca attacccgaa ggcaccctgt tctatccggc
     6901 acacgaatac accgccgcca acctgcgttt cgccgcccat atcgagccgg acaacgccga
     6961 cattcagacg gcattgaagg cggcggaacg cacgcccacc ctgcccgtta ccctcgcgca
     7021 cgaacgccgc gtcaacccgt tcttacggac agaaatcccc gccgtccgcc aacgtgccga
     7081 agccctggtc ggcaaaacgc tgaacagcgg tttggaagta ttcgccgccc tgcgcgaact
     7141 gaaaaacgcc taccgctgac ctgccctccg aaaaatgccg tctgaaaccc gcgtttcaga
     7201 cggcatttgc gttaaaaata gtaaaccgtt tcaaaaggga gtagaatagt gctgtttcca
     7261 atcccgtgcc tgatttttcg ggcttttatt atggaccttc ccagttcgtt tttactgaac
     7321 accccttccg attccaaacg acaataacca tcccgccgga atgcctcccc gcacacggcg
     7381 ggcggagcat ttatgagcat cgaacacact cctccgacac acgacggcga aaccggtcaa
     7441 aaccatgccg aacgcccttc cgccgatttc gaccgtgtcc actccctctg cgaaatcctc
     7501 gaacctgctt ttgaacaaat cgaaaacggc acaccgctcg aagatgcgcc gctgcgcgac
     7561 aaactgaccg agctgaccgt cctcttggcc gagttgcacc ctgccgacgt ggccgacgtc
     7621 ttggagtccc tgccgccgcg cgaacgcaat atcgtttggc ttctggtcaa acccgaagac
     7681 gacggcgaag tattgctgga agtatccgac gcggtgcgag aaacgctgat cgagtcaatg
     7741 gacaaggacg aattgttggc ggcggtcgat gatttggatg cggacgaatt ggcggagctg
     7801 gcagacgatt tgccgcacca agtggtttat gaagccttac agacgcgcga tgaggaagag
     7861 cgcgcccaag tcaaggcggc aatgtcgtat gaagacaacc aagtcggtgc gattatggac
     7921 ttcgagttgg tcagcatccg cgccgatgtc gcctgcgaag tggtgctgcg ttatctgcgc
     7981 cgcttcgaca gcctgcccga ccataccgac aagatttttg tcgtcgatga aaacgacgtg
     8041 ctgcagggcg tgctgcccat ccgcaaactt ttggtcgccg atcccgaaga tttggtggaa
     8101 aacgtgatgg cgaaagatgt cgtgcgtttc cgcgccgagg acgacgtgga agaagcggcg
     8161 caggcgtttg aacgctacga cttggttacc gcgcccgtcg ttgatgaaaa caaaaagctc
     8221 atcggcagga ttaccatcga cgagatggtg gacgtgatcc gcgaagaatc cgaagcggat
     8281 atgttgaaca tggcaggttt gcaggaagag gaagacctgt tcgcccccgt cttggattcc
     8341 gtcaaaaacc gctggatgtg gctcgccgtc aacctctgca ccgccttcct cgccagccgc
     8401 gtcatcggcg cgtttgaagg cagtatcgaa aaaatcgtcg cactcgccgc gctgatgccc
     8461 atcgtcgccg gcatcggcgg taactcgggc aaccagacga ttaccatgat tgtccgcgcg
     8521 atggcgatgg ggcagctgac ggatatgcag gcgggaaggc tgttgaaaaa agaagtcggc
     8581 gtcgccttgg tcaacggcat tatttggggg acggtgatgg gagcggtttc gtggctgctt
     8641 tacggcagcc tcggcatcgg tttggtcatg gttgccgcga tgacgctcaa cctcctgctg
     8701 gcggcaacgg tcggcgtatt gattcccgtg gtaatggaaa aattcggacg cgatcccgcg
     8761 ctgggcagct cggtgctgat taccgccgtt accgactccg gcggcttcct gattttcttg
     8821 agtctcgcca cgctgttcct gctttaaatg ccgtcccgac ccgcgcaaaa atgccgtctg
     8881 aagcagaagc tgcttcagac ggcattcgac cattcatcct tgttgctcaa gattattgga
     8941 cggtatgtcg gggcagccct ttggcaacgc cgaccacatc ctccccgaac agcgcgttga
     9001 catcggtttc atcaaacacg tatttgctgt ggcagaagtc gcaatcgact tcgatgctgc
     9061 cttgttccgc caccacgccg ccgacttctt ccccgcccag catcaacagc atatcgctga
     9121 ccttgccccg agaacaggta catgaggatt caaacgtttc cggctcgaac acgcgcggcg
     9181 gcgtttcgtg gaacaggcgg tataaaacgt gttgcgcgtc caattctgcc agctcctccg
     9241 ccgtcagcgt gcgcgccagc gtactgacgt gttcccatgc ctcttcatcc aacacctctt
     9301 caggcagacg ctgcaccagc agcccgcccg ccgcttcatc gcttgcagac agggcgatgt
     9361 gcgtatcaag ctgttcggaa cgtttcatat agttcaccaa catttgcgcg atgctgccgc
     9421 cttccaaagg cactacgccc tgccagggtt cgccgtcttt gggttgcagc gtcaatacga
     9481 acacgccgtt gccgcccaaa aggtcgccga ggctttcgtc atcggctatt tctgcggttt
     9541 cgtcccaccg cgcggttgca cggacggtac ggtcggaagt cgcttccgca accagcattt
     9601 tcagttttcc ctgcccctga acctgcacaa tcagcgtgcc ttcgttttta aggttgcccg
     9661 acagcagaac gccggcagcc agtagctctc ccaaggcgca gctgatggcg gcgggatagt
     9721 ttttctgttt tacaatgtgc tgccacacgt tttccagacg gacgtgcagc ccgcgcacgg
     9781 gcatatcgtc gaagataaag cgggtacgcg catcggcgcg gttgatggcg gtttgattca
     9841 tgattttctc tgactgattg ttcggatggc ggctatatgg ttgcggtcgg cgcgaaaaca
     9901 aggcggacgg cggatgcgct tcccaaattc tcaataaatt atataaaaat caacatatta
     9961 actcaatcta acaagccgtt ttttgccaaa cagccgtttt tttatataca atcaacaaga
    10021 tattttcgac tgatacagca taacatcgca cggcggcacg atgcctcctg cgcggaaaca
    10081 ccgatatgga ttcttttttc aaaccggcag tttgggcggt tttgtggctg atgtttgccg
    10141 tccgccccgc ccttgccgac gagttgacca acctgctcag cagccgcgag cagattctca
    10201 gacagtttgc cgaagacgaa cagcccgttt tacccgtcaa ccgagccccc gcccggcggg
    10261 cgggcaatgc cgacgaactc atcggcagcg cgatggggct taacgaacag cccgttttac
    10321 ccgtcaaccg agcccccgcc cggcgggcgg gcaatgccga cgaactcatc ggcagcgcga
    10381 tggggctttt gggtattgcc taccgctacg gcggcacatc ggtttctacc ggttttgact
    10441 gcagcggatt catgcagcac atcttcaaac gcgccatggg catcaacctg ccgcgcacgt
    10501 cggcggaaca ggcgcggatg ggcgcacccg ttgcccgaag cgaattgcag cccggggata
    10561 tggtgttttt ccgcacgctc ggcggcagcc gcatttccca tgtcggactt tatatcggca
    10621 acaaccgctt catccacgcg ccgcgcacgg ggaaaaatat cgaaatcacc agcctgagcc
    10681 acaaatattg gagcggcaaa tatgcgttcg cccgccggat caagaaaaac gacccgtcac
    10741 gctttctgaa ctgatttccc aaggaatacg caatgagtat gcccgaaatg cccaaatggt
    10801 acggcgatga cggacagatc gtatcctgta ccgaaaaggt caaagtgatg tccgaaaata
    10861 tggccgagct gtatcagacg gcacaagacg cgtttgaaga cgcgctgctg atgggttgcg
    10921 gcgaacggca gttgcgcgct tacctgctcg cgctgattga aggtttggaa aatccctacc
    10981 gcaaagtctg aacacgcccc ggttgctgcg gcacggttta tccgtgccgt ttttgcgttt
    11041 gtgcgcggct tcggcttttc agacggcata tttgacgtta tgattaaaca gttaacaaga
    11101 tttatcacaa cgccgtcaaa agacagacac acaacatgaa catcatccgc gcgctcctca
    11161 tcatcctcgg ctgcctcgcc gccggcgaaa ccgccgtttt cctagcaggc atcaaactgc
    11221 ccggcagcat cgtcggcatg ggcgtgctgt ttgcgctttt gcaggcgggt tgggtcaaaa
    11281 cgtcttggct gcaacagctt accgacgcgc tgatggcaaa cctgacgctg ttcctcgtgc
    11341 cgccctgcgt ggcggtcatc agctatttgg atttgattgc cgacgattgg ttttcgatac
    11401 tggtttccgc ctccgccagc actttgtgcg tactgctggt tacgggcaag gttcaccgct
    11461 ggatacggag cattatctga tgagcgaaat cctcaggcag cccagcgttc tgcttttcct
    11521 cacgcttgcc gtgtacgcgc ttgcgattat cgtgcgcacg cgcacgggca atatcttctg
    11581 caaccccgta ctcgtcagca ctatcgtgct gattgcctac ctgaaaatcc tcggtatcga
    11641 ttatgcggtg taccacaacg ccgcgcaatt cattgatttt tggctgaaac ccgccgtcgt
    11701 cgtgcttgcc gtgccgctct accaaaaccg ccgtaaaatc ttcaaccagt ggctgcccgt
    11761 catcgtttcg cagcttgcgg gcagcgttac gggcattgtt acggggatgt attttgccaa
    11821 gtggctgggc gcggaacgcg aagtcgtcct ctcgctcgcg tccaaatctg ttaccaaccc
    11881 catcgccatc gaaatcaccc gctccatcgg cggcattccc gccattaccg ccgccaccgt
    11941 catcattgcc ggtctggtcg gacagattgc cggttacaaa atgttgaaga acacggtcgt
    12001 catgccctcg tccgtgggta tgtcgctcgg cacggcttcg cacgcgatgg gcattgccgc
    12061 ctcgctcgaa cgcagccgcc gcatggcggc atacgcgggg atggggctga cgttcaacgg
    12121 cgtgctgacc gcgctgattg cgccgctgct tatccccgtt ttggggttct gaacccgttt
    12181 cagacggcat ttcaacccat gccgtctgaa cgccgacaca ctcgcaagga gaaccgttat
    12241 ggctgtcaac ctgaccgaaa aaaccgccga acaactgccc gacatcgacg gcattgccct
    12301 ctacaccgcc caagcaggcg tgaagaagcc cgggcatacc gacctgacac tgattgccgt
    12361 agccgccggc agcaccgtcg gtgcagtctt cacgaccaac cgtttctgtg ccgcgcccgt
    12421 ccacatcgcc aaatcgcacc ttttcgacga agacggcgtg cgcgccctcg tcatcaacac
    12481 gggcaacgcc aacgcgggta cgggcgcaca gggcagaatc gatgctttgg cagtgtgtgc
    12541 cgccgccgcc cggcaaatcg gctgcaaacc gaaccaggtg atgcccttct ccaccggcgt
    12601 gattctcgaa ccgctgcccg cagacaaaat catcgccgcc ctgcccaaaa tgcagcctgc
    12661 cttctggaac gaagcggcac gcgccatcat gaccaccgac accgttccca aagccgcctc
    12721 gcgcgaaggc aaggtcggcg accaacacac cgtccgcgcc acaggcattg ccaaaggctc
    12781 gggcatgatt catcccaata tggcgaccat gctcggtttc atcgccaccg atgccaaagt
    12841 ttcccaaccc gtcctccaac tgatgacgca ggaaatcgcc gacgaaacct tcaacaccat
    12901 caccgttgac ggcgacacca gcaccaacga cagcttcgtc atcatcgcca ccggcaaaaa
    12961 cagccaaagc gaaatcgaca acatcgccga cccgcgttac gcccaactca aagaattgtt
    13021 gtgcagcctt gcgctcgaac tcgcccaagc catcgtccgc gacggcgagg gcgcgaccaa
    13081 gttcatcacc gtccgcgtcg aaaacgccaa aacctgcgac gaagcccgcc aagccgccta
    13141 cgccgcggca cgttcgccac tggtcaaaac cgcctttttc gcctccgacc ccaacctcgg
    13201 caggctgctc gccgccatcg gttatgccga cgttgccgac ctcgataccg acctcgtgga
    13261 aatgtatctc gacgatattt tggttgccga acacggcgga cgcgccgcaa gctacaccga
    13321 agcacaaggg caggcggtga tgtcgaagga cgaaatcacc gtccgcatca agctgcatcg
    13381 cggacaagcc gccgccaccg tctatacctg cgacctgtcg cacggatacg tttccatcaa
    13441 cgccgactac cgttcctgac ccgacacggc ttcagacggc atacataaaa tgccgtctga
    13501 accgtcggac aacataccat gacctccaca ttcccccgcc gcctcgcccg caaaatccgc
    13561 caaacccgcc gcctgtcgcg caaaagcatc gcctttctgt tccttctggc aggttcggca
    13621 ctcgtcgccc tgaccgcact gttttttgcc catcttgccg attttgcgct ggaactgaac
    13681 gccaaactgg ttcaacaata cccgtggttc gcatggattg ccctgccgcc gggactgccc
    13741 gtcatcgtct ggctcacacg caaattcgcc cccttcaccg ccggcagcgg catccctcaa
    13801 gtcatcgcct cactgtcgct gccctacggc gcacaaaaaa cgcggctgat acgcctcggg
    13861 caaacgctgc tgaagattcc gctgaccttt ttgggtatgc tgttcggcgc gtccatcgga
    13921 cgcgaaggcc cgtccgtgca ggtcggcgcg gcagtaatgg gcgcgtgggg cgcgtggtgc
    13981 aaaaaacacg gtttggcgtt taagggtatg caggaaaacg atttgatggc ggcgggcgcg
    14041 gcgggcggtt tggcagcagc gttcaacgcg ccgctggcgg gcgtgatttt cgccattgag
    14101 gaactggggc gcggcatcat gctgcgctgg gagcggcaga ttctgctcgg cgtactcgcc
    14161 gccggtttca tacaggtcgc cattcagggc aacaacccgt atttttcggg tttcaacggc
    14221 ggcgttttgg aaaatatttt gatgtggatc gtggcggcgg ggctggtttg cggcgcggct
    14281 ggcggcatat ttgcccgtat gctctatcgc ggtgcggcgg cgtttgcacc gcgcaagata
    14341 cgcggcttca tccgcaaccg tccgctgctg ctggcggcac tgatggggct gctgctcgcc
    14401 ctgctcggca cgttctacca aggcaaaacc tacggcaccg gctaccacga agccgcccaa
    14461 gccctgcacg gcatctacga agcccccgtc ggactcgccg ccgccaaatg gctcgccacc
    14521 gtattcagct attgggcagg cattccgggc ggcattttca ctccctcgct gaccataggt
    14581 gcggttttgg gcgagcatat cgccgccatc gccgacatat cgcagggtgc aaacatcatc
    14641 gtcctcatct gcatggcggc atttttagcg ggcgcgacac aatcgccgat tacttccgcc
    14701 gtcgtcgtca tggaaatgac cggcgggcaa agcctgctgt tttggatgct gattgcctgc
    14761 attttcgcct cgcaggtttc gcgccagttt tcgccgcgtc cgttctacca cgcatcggga
    14821 atgcgcttcc gccggcgcgt gcttcaggaa accgccgccc aaaccggcaa tgcgcccgca
    14881 agaccgcaag ccgcaaacag caaaacggga atgccgtctg aaaattaaaa cgcccccgat
    14941 caaacgccgg cagccgcctt gattcaaata ccgttccgct gcgcttgaaa tttcagcaac
    15001 aatgccgtct gaacgacaga atgcggtttt cagacggcat ttccccatcc cgatattgct
    15061 caaacaaaac cgaagcgttt gctataattg cactttttac cgcacacgca cgcccatgtt
    15121 tcccgatttc tcccaaaccc tctccaaaga ccgccacttc ctgcaatccg ccttcaaaaa
    15181 tcccaacaaa tacggcggct tgtccaaaat cgaagaaaaa taccgaaaat cgcacgaaat
    15241 cttcttgaaa cgtttggccg cattgccaaa acccgaattc gacgacaccc tgcccgttca
    15301 cgaaaaactc gaagaaatca aaaaaaccgt tgccgaaaat caggtaacga ttatctgcgg
    15361 cgaaaccggt tcgggcaaaa ccacgcagtt gcccaagatt tgtttggaac tcgggcgtgg
    15421 ggcggcagga ttgatcgggc atacccagcc gcgccgtttg gccgcgcgtt cggtggcgga
    15481 gcggattgcc gaagagctga aatccgaaat cggcagcgcg gtcggctata aagtgcgctt
    15541 taccgaccac acctcgcgcg atgcctgcgt caagctgatg accgacggca tcctgctggc
    15601 ggaaacccag accgaccgtt atctcgccgc ctacgacacg attatcattg acgaagcgca
    15661 tgaacgcagc ctgaacatcg acttcctctt gggctaccta aaacaactcc tgccgcgccg
    15721 ccccgatttg aaagtcatca tcacatcagc caccatagac gcagaacgct tctcccgaca
    15781 cttcaacggc gcgcccgtac tggaagtgag cgggcgcacc tatcccgtcg aaatcctcta
    15841 ccgaccgctg accggcaaag acgaagacga cgcagaagtc gagctgaccg acgcgattgt
    15901 cgatgccgcc gacgaattgg cacgctacgg cgaaggcgat attttagtat tcctgccggg
    15961 cgaacgcgaa atccgcgaag cggcagaagc cctgcgcaaa tccacactgc gccgcaatga
    16021 cgaaatcctg cccctgtttg cacgcctgtc gcacgccgaa cagcacaaaa tcttccaccc
    16081 ttcaggcgca aaatgccgca tcgtgctggc gaccaacgtc gccgaaacct cgctcaccgt
    16141 gccgggcatc aaatacgtca tcgacaccgg cctcgcgcgc gtcaaacgct attccgcgcg
    16201 ggcaaaagtg gaacaacttc acgtagaaaa aatctcccaa gccgccgccc gccaacgctc
    16261 cggccgctgc ggacgcgtct cagcaggcgt gtgtatccga ctgttttcag aagaagattt
    16321 taacagccgt cccgaattta ccgatcccga aatcgtccgc agcaacctcg ccgccgtcat
    16381 cctgcgcatg gcatcgctga acttgggcga cgtagcagca ttcccgtttt tagaaatgcc
    16441 cgactcgcgg tatatcaatg acgggtttca ggtgttgttg gaattggggg cagtggaggt
    16501 tgtctgaaaa caggcagatc gggcgtttat gcccaacgag aaccttattg tcactattat
    16561 tagggattaa tacaatgaat ataaaagaat ttatgtctaa ctataccaac catcccgttc
    16621 tctttattgg aacaggtatg agtttgagat acttagataa ttcatatact tgggatggtt
    16681 tattatctaa aattgcaata gatttatttg gagatgatag ggaatatttg aacatcaaat
    16741 cacggtactg tgaagatggt agattccaat atgaagagat tgcagaggaa ttacaaagta
    16801 aatttgataa agttttagaa aatgaccctg atggtagatt taaagaaata aatgataagt
    16861 tctttgaaaa tatgagggcg ggaaacacct tgagcaggtt taagatttat atatccaccc
    16921 tgctctcaca attgaattat aaagataatt ctaatacaga attatctgaa ttaaaaaaag
    16981 cgagaaaaaa tgtagggtca atcattacaa caaattatga taaattagcc caagatattt
    17041 ttgaatttaa cccactaatt ggtaatgata ttcttttaag caacccttat ggctcagtat
    17101 acaaaataca tggttgtgtg gacgatccat caaaaattat tattaccaaa aaggattatg
    17161 agaaatttaa agaaaaatat gaacttatta gagcccaact attatcgtta tttattcaca
    17221 atccaatcat atttcttgga tataatgttg gtgacgaaaa tatcaaagaa attttaaaaa
    17281 caatctttac ttatgtagaa ccaaactctc cttcagctaa taaaattcgt aggaattttt
    17341 tactcgtaga atatgaacct gagtctaaca atgaagatat tgttgaacat gatatagata
    17401 taactggatt ctctactatc cgtattaata aaatcaaaac agataacttc tcacaaattt
    17461 ataaagctct tgcagaacta acactaccaa tctcagctat ggatgtacgt aaatttcaat
    17521 ctatagcaaa ggagatttat actggcggta acattaaagt tagctttacg gaagatatgg
    17581 acaatttaaa taatagcgat aaagtggttg ctattggttc aactaaaact atcagctaca
    17641 actttcaaac aacatcagag atgatgtcaa attatttcaa aataattgaa gaggaaaatt
    17701 cacaacttct aaaattaata gataaacata gtatagcatc tacgcaatat tttcctattt
    17761 atggatttag taggatatgt tctgatatac ataaagaagc tgtactaaaa cgccaacaaa
    17821 aagaaaaatt agatcatttt attgaagaaa taaataggcg ttgtaaaaat aatcattcat
    17881 ccatccaatc aattttagat gatgaaaata tttcagacac atataaaaac gatgcgattg
    17941 cttggggaat atggaataac caactttcag aagatgaggt tgaaaattat ttaaaaaatt
    18001 ttgtgaataa aaaaaatacg cactataaaa gactactatg tatgttcgat tataaaaaat
    18061 atgcggatac tgtctaaact ctaaaaaata aggtcgggca tttatgtccg acctacaaaa
    18121 ctatgcaaaa cactaaaaat caagcacgca gctcaggcat tcatgcccaa cacaatccga
    18181 caaatgacaa accactttca gacaacctcg ttttttgaaa aacaatctgt aaacagatag
    18241 ctgctgaaga ataccgttgc cgagccccaa aacccgtcct gcaactttta ttgtgaactg
    18301 cccattatga aaaaatccct tttcgttctc tttctgtatt catccctact taccgccagc
    18361 gaaatcgcct atcgctttgt attcggaatt gaaaccttac cggctgcaaa aatggcggaa
    18421 acgtttgcgc tgacatttgt gattgctgcg ctgtatctgt ttgcgcgtta taaggcttcg
    18481 cggctgctga ttgcggtgtt tttcgcgttc agcatgattg ccaacaatgt gcattacgcg
    18541 gtttatcaaa gctggatgac gggtattaac tattggctga tgctgaaaga ggttaccgaa
    18601 gtcggcagcg cgggcgcgtc gatgttggat aagttgtggc tgcctgcttt gtggggcgtg
    18661 gcggaagtcg tgctgttttg cagccttgcc aagttccgcc gtaagacgca tttttctgcc
    18721 gatatcctgt ttgccttcct aatgctgatg attttcgtgc gttcgttcga cacgaaacaa
    18781 gagcacggta tttcgcccaa accgacatac agccgcatca aagccaatta tttcagcttc
    18841 ggttattttg tcggacgcgt gttgccgtat cagttgtttg atttaagcaa gatccctgtg
    18901 ttcaaacagc ctgctccaag caaaatcggg caaggcagta ttcaaaatat cgtcctgatt
    18961 atgggcgaaa gcgaaagcgc ggcgcatttg aaattgtttg gttacgggcg cgaaacttcg
    19021 ccgtttttaa cccggctgtc gcaagccgat tttaagccga ttgtgaaaca aagttattcc
    19081 gcaggcttta tgacggcagt atccctgccc agtttcttta acgtcatacc gcacgccaac
    19141 ggcttggaac aaatcagcgg cggcgatacc aatatgttcc gcctcgccaa agagcagggc
    19201 tatgaaacgt atttttacag tgcccaggct gaaaaccaaa tggcaatttt gaacttaatc
    19261 ggtaagaaat ggatagacca tctgattcag ccgacgcaac ttggctacgg caacggcgac
    19321 aatatgcccg atgagaagct gctgccgttg ttcgacaaaa tcaatttgca gcagggcagg
    19381 cattttatcg tgttgcacca acgcggttcg cacgccccat acggcgcatt gttgcagcct
    19441 caagataaag tattcggcga agccgatatt gcggataagt acgacaacac catccacaaa
    19501 accgaccaaa tgattcaaac cgtattcgag cagctgcaaa agcagcctga cggcaactgg
    19561 ctgtttgcct atacctccga tcatggccag tatgtgcgcc aagatatcta caatcaaggc
    19621 acggtgcagc ccgacagcta tattgtgcct ctggttttgt acagcccgga taaggccgtg
    19681 caacaggctg ccaaccaggc ttttgcgcct tgcgagattg ccttccatca gcagctttca
    19741 acgttcctga ttcacacgtt gggctacgat atgccggttt caggttgtcg cgaaggctcg
    19801 gtaacaggca acctgattac gggcgatgca ggcagcttga acattcgcaa cggcaaggcg
    19861 gaatatgttt atccgcaata agcagcgtga aaaccaataa agacaaattt agatgatgtc
    19921 ggggaagatg cccgaccgac aagactatgc aaaatatgaa aaaccaagta cgcggatcag
    19981 gcatggatgc ccgatccaac ccggccaatg tttcagacgg cctgcaaaac agttcgggcc
    20041 atatcggtac caacacgcgc taccgcctga ccaagctcgg cgaacaaatg gcgcgcctgc
    20101 ccatcgatcc gaaaattgcg cgcattttgc tggcggcgaa gaaacacgac tgcatggcgg
    20161 aaatattggt gattgcgtct gcgctgtcga ttcaagaccc gcgcgaacgg ccgctggaag
    20221 cgcgcgatgc cgcggccaag gcgcatgaac gctttaccga caagcaatcc gatttcctta
    20281 cctacctgaa tatttgggac agtttccagc gcgagcgcga caaaggtttg tccaacaagc
    20341 agttggtgca atggtgccgt caatattttc tgtcgcacct gaggatgcgc gagtggcgcg
    20401 agctgcacca ccagcttgcc caaaccgcga ttgaaatggg cttgaccacc aaagaagctg
    20461 cgtttagacg acctcccgaa gtcaagcaac tgacgtcgtc tgagaatgcg ggcgaccaag
    20521 acctatctgc caaactcaaa caaaaacaac tggataagaa acaacaccgc acccaaatcc
    20581 gcgctaccaa agaagcgggc tacgaacaaa tccaccgcgc cctgctcacc ggcctcatcg
    20641 ccaacgtcgg catgaaatcg cccgacagca acgactacac cggcgcgcgc ggcagccgct
    20701 tccacctttt ccccgcctcc gccctgttca aagccaagcc caaatgggtg atggcggcag
    20761 aattggttga aaccacgcgc ctttacgcgc gcgacgtcgc cgttatccag cccgaatgga
    20821 tcgagcagga agccccgcac cttgtccgct accattattt cgagccgcac tgggaacaaa
    20881 aacgcggcga agtcgtcgcc agcgaacgcg taacgcttta cggcctggcc gtattgccgc
    20941 gccgccccgt gtcttacggt aaagttgcgc ccgaagaagc acgtgaaatc tttatccgca
    21001 gcgcgttggt ggcgcaggaa tacgatttga aagcggattt ttttgtccac aacaaaaagc
    21061 tgattaaaga aattaccgaa ctcgaacaca aatcgcgcaa gcaagacgta ttggtcgatg
    21121 acgaagccct gtttgcgttt tatcacgaac ggctgcccga tttttatatg gcggattcgg
    21181 tttcagaggg cctgtgtcct gcaaatccgc agcaaaccac cccctccccc gtgggggaag
    21241 gctggggaga gggcaaaaca gttgccgcac aaaccaactt ttccgcaacc tcagcaagcc
    21301 ctctccctaa ccctctccca caggagaggg aacagagtgc ctcagtttca acggtttcag
    21361 gcagtctgaa aacaatgtct tgcgaagcaa ggctgaattt ctgcgaagct aaaactaaaa
    21421 ccgaaagcag cctgcactcc caaaggctat ctgaaaacta caccccgcca ttttcagacg
    21481 gcctgcgtcc tgcaaacccc cagcaaacca ccccctcccc cgtgggggag ggctggggag
    21541 agggcaaaac agttgccgca caaaccaact tttccgcaac cgcagcaaac cctctcccac
    21601 aggagaggga acagagtgcc tcggctttaa cagtttcaga tgaccccaaa cccaaaaagc
    21661 agcctgcatc tcaaaaaggc cgtctgaaac ctttgcccct tgccgatatc cgcacctttg
    21721 aagcctggct caaaaccgcc gagcgcgaca acccgcgcct gttgttcctc agccgcgacg
    21781 acctcatgca gcacgccgcc gcgcacatca ccgaagaaca attccccaaa cactggcaga
    21841 ccgcagacgg caaattcaaa ctcagctacc gcttcgagcc gcaccacccg ctcgacggcg
    21901 ttaccctcac cctgccgctc accgtgctca accgcatcag ccccgccgcc ctcgaatggc
    21961 tcgtgcccgg catgatacgc gaaaaaatcc agctacaaat caaagcactg cccaaacaaa
    22021 tccgccgcat ctgcgtaccc gtacccgaat tcatcaccca atttttaagc caaaaccccg
    22081 accgcaacgc ccccatcctg ccccagctcg cccaagccat cgccaaaacc gcaggcgaca
    22141 tccgcatact cgagcaaatc aaccaagacg aatgggccgc gttcaggctg cccgaacact
    22201 gctatttcaa cctccgcatc atcgacgacg gcggacaaga gttagccatg ggtcgcgatt
    22261 taatccaaat ccaacaacaa cttggcaaag ccgccaccac caccttccgc gacaacaccc
    22321 aagaattcga gcgtgacaac gttaccgcat gggacatcgg catcctgccc gaatccatca
    22381 aattcgcccg cggcaaacaa cagctcaccg gctacctcgg cctgcaaaaa gaaaaagacg
    22441 gccgcatcgc cctgcgcctg ttcgacacgt ctgccgccgc cggacacgcc cacagattag
    22501 gcgtgattga actcatgaaa ctacaactaa aagaacaagt taaagatctg aacaaaggca
    22561 tccaaggctt cacccaagcc gccatgctgc tcaaacacat caacgccgac accctgcgcg
    22621 acgacctcac ccaagccgtc tgcgaccgcg cctttatcgg cgaagacaag ctgccgcgca
    22681 acgaaaaatc cttcaaagaa caaatcaaac gcgcccgcag ccgcctgccc gccgtcaaag
    22741 aagccctcag ccgctacctg caggaaaccg cagccgccta cgccgaactg aacggcaaac
    22801 tcggcaaaca cccattgacc cacctcctaa gactacgcct gcaaaccctg cttgcacccg
    22861 gcttcgccac ccgcaccccg tgggcacaat ggccgcgcct ccccatctac ctcaaagcca
    22921 tgaccctgcg cctcgaaaaa tacagcagca accccgcccg cgacgcagcc cgcgaagccg
    22981 atatccaaga gctggaacaa atgtggcagg aaaaaactga cggcttggcg aaacaaggac
    23041 agcccgtttc agacggcctc gccgcgttta aatggatgat tgaagaattg agagtgtcgc
    23101 tgttcgcgca ggagttgaag acgccgtatc cggtgtcggt aaagcggctg ttgaagatgt
    23161 g
//
LOCUS       DAXFHF010000032        22842 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXFHF010000032 DAXFHF010000000
VERSION     DAXFHF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22842
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.032"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(41..211)
                     /locus_tag="ACFIRM_001132"
     CDS             complement(41..211)
                     /locus_tag="ACFIRM_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC3028775.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1420)
                     /locus_tag="ACFIRM_001133"
     CDS             complement(344..1420)
                     /locus_tag="ACFIRM_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3028776.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIGRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRVVKEKYLA
                     DILQGKVLAGMSNTVKHLAGIEKHNLQAECVEGGYTVNGALPWVSNIGEDHIWANTAQ
                     IGGGYVMFITGGQREGVSLQNCPEFCALEGTHTFSLNFKDVFIPDEDIIAAPEQFAGY
                     IQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAWAE
                     TERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVGEA
                     MFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1558..2106)
                     /locus_tag="ACFIRM_001134"
     CDS             complement(1558..2106)
                     /locus_tag="ACFIRM_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3028777.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVLKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPELQAYA"
     gene            complement(2246..2617)
                     /locus_tag="ACFIRM_001135"
     CDS             complement(2246..2617)
                     /locus_tag="ACFIRM_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC3028778.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2802..4493)
                     /gene="dld"
                     /locus_tag="ACFIRM_001136"
     CDS             complement(2802..4493)
                     /gene="dld"
                     /locus_tag="ACFIRM_001136"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC3028779.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLEQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4590..5009)
                     /locus_tag="ACFIRM_001137"
     CDS             complement(4590..5009)
                     /locus_tag="ACFIRM_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028780.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5232..5328)
                     /gene="ffs"
                     /locus_tag="ACFIRM_001138"
     ncRNA           complement(5232..5328)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFIRM_001138"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5589..9422
                     /locus_tag="ACFIRM_001139"
     CDS             5589..9422
                     /locus_tag="ACFIRM_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC3028781.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKAVERFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARATHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9580..10415
                     /locus_tag="ACFIRM_001140"
                     /pseudo
     CDS             9580..10415
                     /locus_tag="ACFIRM_001140"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
     gene            complement(10560..10742)
                     /locus_tag="ACFIRM_001141"
     CDS             complement(10560..10742)
                     /locus_tag="ACFIRM_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028782.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10915..11046)
                     /locus_tag="ACFIRM_001142"
     CDS             complement(10915..11046)
                     /locus_tag="ACFIRM_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028783.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(11305..12449)
                     /locus_tag="ACFIRM_001143"
                     /pseudo
     CDS             complement(11305..12449)
                     /locus_tag="ACFIRM_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12774..13250)
                     /gene="greA"
                     /locus_tag="ACFIRM_001144"
     CDS             complement(12774..13250)
                     /gene="greA"
                     /locus_tag="ACFIRM_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC3028784.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            complement(13514..13642)
                     /locus_tag="ACFIRM_001145"
     CDS             complement(13514..13642)
                     /locus_tag="ACFIRM_001145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028785.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13641..14942
                     /gene="aroA"
                     /locus_tag="ACFIRM_001146"
     CDS             13641..14942
                     /gene="aroA"
                     /locus_tag="ACFIRM_001146"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC3028786.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHVGELQNNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(15020..15553)
                     /locus_tag="ACFIRM_001147"
     CDS             complement(15020..15553)
                     /locus_tag="ACFIRM_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3028787.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15610..17136)
                     /locus_tag="ACFIRM_001148"
     CDS             complement(15610..17136)
                     /locus_tag="ACFIRM_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC3028788.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(17248..18789)
                     /locus_tag="ACFIRM_001149"
     CDS             complement(17248..18789)
                     /locus_tag="ACFIRM_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC3028789.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            18937..19716
                     /locus_tag="ACFIRM_001150"
     CDS             18937..19716
                     /locus_tag="ACFIRM_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC3028790.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19713..20414
                     /locus_tag="ACFIRM_001151"
     CDS             19713..20414
                     /locus_tag="ACFIRM_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC3028791.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20411..21865
                     /locus_tag="ACFIRM_001152"
     CDS             20411..21865
                     /locus_tag="ACFIRM_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC3028792.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIVITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(22014..22433)
                     /gene="fitB"
                     /locus_tag="ACFIRM_001153"
     CDS             complement(22014..22433)
                     /gene="fitB"
                     /locus_tag="ACFIRM_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC3028793.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22433..22669)
                     /gene="fitA"
                     /locus_tag="ACFIRM_001154"
     CDS             complement(22433..22669)
                     /gene="fitA"
                     /locus_tag="ACFIRM_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC3028794.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         5289 a         6110 c         6205 g         5238 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtcaatc acgcccgcgc
      721 cgataccgat ttgcaggagg atgaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg gaataaatac gtctttgaaa ttcaggctga
      841 aggtgtgcgt gccttcgagg gcgcaaaatt cggggcagtt ttgcaggctt acgccttccc
      901 tttgcccgcc tgtgatgaac atgacgtagc cgccgccgat ttgggcggta ttcgcccaaa
      961 tgtggtcttc gccgatgttg gacacccacg gcagcgcacc gttgactgta taaccgccct
     1021 ccacgcactc ggcttgaagg ttgtgttttt caatgccggc aaggtgtttg acggtattgg
     1081 acatgcccgc caatactttg ccttgcagga tgtcggcgag gtatttttct ttgacgaccc
     1141 ggttgggcgt ttggtgcaga taccacgcgc aagccgcctg acaccacgcg ctgaaggagg
     1201 tcgcgccgca ttctttgccg atttcgcgca agacggcgat tcgtgttgcc aagcctaagc
     1261 cgttgccgcc ttcggcttct ataccgactg cgccgattgc gccgagttcg cgcataaatg
     1321 cttcggggta gtatcctttg cggccgatgt cgtcgacgag gggtttgagc ttggttttga
     1381 cgagttcggc aacgttggca agcagggttt gggcgttcat ctttgttcct taaggttttt
     1441 ggggggaaat cggggacacg cctgatgcgc ggctgctttt cccgccgctt gggcggcttg
     1501 tttttgtttg agggggtgtt tttgaaaaac cgcccgatat tcgggcggtt tgccgtatca
     1561 ggcgtaagcc tgcaattcgg ggttgatttc ggtttgggcg aggttgttgg cgtagttgca
     1621 caaagttgcc aaggctacgc ccattacgac ttcgactgcc tgctgccggt tgtagcccgc
     1681 atcgaaaaat gctttgagtt cgtcgtcgga tactgcgcct tttttcgcca ttacggcttg
     1741 ggtaaaggcg gcaagcgcgc cgagtttggc atcgtcaaat tcacctgccg ccaaagcgcg
     1801 ggcggcattg agggattgct cggacaggag ttttttcagg gttgcgagtt tggtgtgccc
     1861 tgccacgcag aagccgcatt ggttggtgcg tgcggcaatg atccggatca cttcgacttc
     1921 gccggcggtc aggctgttgg cggcgttgag cttgccgact tcttggtaaa acgccaaggc
     1981 ttcgggggcg tttgccaata cgccgatgag gttggggata aagccgttgt ttttgagtac
     2041 ggcctctacg cgcggtttgg cggcttcggg ggcggtttcg agggtgtgta cggttaaacg
     2101 tgccattttc tttccttgtt cgcaaatatt ggggacgggc gcatggtacg catttcggaa
     2161 cggaatagga aagactgtgc cacaaacaaa aagttataag aaatgccgcc tgaagccttt
     2221 cagacggcat tgccgggaaa cacgcctacc cgcccccgcg tccgaaatat tgcgccaatg
     2281 cggcttgggg gttggcggct tcgactgttt cgggcaattc ggtatagccg cgcgataaaa
     2341 ggtggcgtgc ataaaggctg ttgccctttg cgccgaacca gacggaaacc gccaagccca
     2401 atacgttaaa cgccgagtct gtggcgggcg tgtcgccgta aaccagttgg gcaaacaccg
     2461 ccaacacgaa tatcgcgccc gtcatcccca gccccaaacc ccacattctt ttgaaacacg
     2521 cccacagcgt gccgaacaaa agacccggcc acgaccagcc ttgtttgacg gcttggggcg
     2581 gcagggcggg atgggtgtag attttgtatg gtttcatcgt gtttcctttt cggttgaaac
     2641 cccgcccttt ggggcggtag gatcagactt tatttgtgag acgcgcgccc gtgtgccgac
     2701 atacggcttg aacatgcccg tcggggaaga cagatccgac aatgccgtct gaacggtgtt
     2761 ttgccttcag acggcattgc ctgccttcaa agcggacgcg cttattccgc ccagtttttc
     2821 tttttgctgg ttttgccgat acccgggttg aagctgttgg tcgggtcgag tttgcggtaa
     2881 aactgtttga gcgcgggctt ggcttcatac aaatggccga cgttgtgttc ggcgggatat
     2941 tgcgcgccgc gttgctctaa gagatgtagc atttcgtgtt ccaacgccat acagtcgttg
     3001 ccttttttga tgatgtaatc ctgatggaaa acgtggcaca taaaatgccc gtaatacaat
     3061 ttatggatga ttttattgtc gatttccggc ggcagttttt caaaccagtc gcggtcgtcg
     3121 cggcgcaggg cgatatccag cgcgaccaag tcctccactt cgtcgtcgtg cacggaacgg
     3181 tagcggatgg cggcggaggc gacggcgaaa cggtgcagca tggcggcttg ggtttcttcg
     3241 gcgttgcatt cgaaaaacgc gccgccgtgg tgtgaaaaat attcttttaa gaacgcgcgc
     3301 gcctcatcca cgccttttcc gcccattttc agaatcaggt ggtgttcgta tttgtcgcgg
     3361 taatcgcgca tggatttggg caggtggtcg ggcaggtatt tgctgacgaa ctgcattgcc
     3421 ttgtcggaaa aatgtttggg caggaagctg acttttttgc cgaacctgtc cacgcgcgcc
     3481 ttcaagtcaa ataatttcgg cagttggtgc gtaccgaatt ttttgatgac gtagaacgtg
     3541 tctttgccgt acacgtcggc aatgtcgaaa gcgtggcggt ggatgtattc gccggaaacg
     3601 ggcaggcttt caaattcgcc caaggcggcg cggcggatgt cggtcagctc gttgatgtcg
     3661 ttcgtgccga tgtagaacac ggcggtttgt ttttcttgcg ggaaggtgtc caaacggacg
     3721 gcaaaaacca tcagcttgcc cgcgcagccc gaggcttcgt aatggcgggc ggggtcggca
     3781 ttgaaacgcg cggcggtcgg ttcgtccact tggcggacgt gttcgcaata ggcgtggtca
     3841 tgtcctttgc ccgcgtcttg tttgatgtct tttttctgat aatgatgacc ttgcaggttg
     3901 gtcaggattt cttcgggcgt gtcgcccaag tctatgccca agtggttgac cagttccaac
     3961 ctgccctctt cgttgatttg ggcgaacaac gccatttcgg tgtaggccgg gccgcgctgt
     4021 accaacgcgc cgccggagtt gttgcacacg ccgcctaaaa cggacgcgcc gatgcaagaa
     4081 gagccgatga ccgaatgcgg ttctcgtccc aaaggtttca gcagcagttc aagctggttc
     4141 agggtcgagc cgggcaggca gacgacttgt tcgttgttgt tgatggtttg gatgatgttc
     4201 atccgcatgg tgttcacaat cacgatgtcg cggtcgtaat cgttgccgtc gggggtcgaa
     4261 ccgccggtca gtccggtatt cgccgcctgc gtaatcacaa tcacgtccgc ttcgacgcag
     4321 gcctgcagaa ttttccacat ttccaaaatg cttcccgggc gcaccaccgc caacgccttg
     4381 ccttcgccga aacggtagcc ttggcggtat tgttcggttt tcgcggggtc ggtgatgatg
     4441 tatttttcgc ctacggtttg ggtcaggctg ctgattaatt gcgaggcgtt cataacactc
     4501 tcctatcggg ataagcggcg gcgcaaatcc cggtacgccg ccgtatgttc aatttggata
     4561 ttttggtgcg gcaagttgca cgctgcaaat tatttttggt taaagaaata atgtaattta
     4621 atatttttgg catttgccgc gttttccaaa tcggccttgt cgctttcgct atcgcccgtc
     4681 atatagacac tgacattatt tccgactttc tttgcatggt ttaaggaata aagtgcggtc
     4741 gccttattaa acatttggct gcctcccgcc acgccgacgt tgatattgtc cgcgccgagc
     4801 atttcaatca tatttgatgc cgaaggcgat ccggatgttt taatcattcc gacagccacc
     4861 gcatcggaaa gcgggttatt cgtcgcggga acctgcacga caacagagcg ttctccctgc
     4921 ggcatttcaa ccatcaaccg gccgacattg acacctgttg aacaagctgc aagcataccg
     4981 gtgagggtta tcattagaag ttttttcatt tgcctactcc tttagtcttt aatcaaaccg
     5041 ccctgttcgt catataactt gaaattgaac catacttcca tttttatgaa gccggttctt
     5101 cattggcctg tatggataca ttgtaatgat gcgttatggc aaagtaaagg aaatgccgtc
     5161 tgaacggctt tcagacggca tcggtgcgat acgggaacgc cggaacatcg aagctccggc
     5221 gtttcaaata gggcggcggg ccaaaccccc ggcactggcg catcggagtg ggctgctggc
     5281 ttccgcccct gacccggtgt tccgatttgc catgcgggga gacccgcctc ggagaaacgg
     5341 cattataacg ggttttctga aaaactcaac cgttttgata cggtcatacg ccggaaacac
     5401 cgcctaaaat ttatatttga taatattgtc aacaatttct caaagcgtta ttttgtttct
     5461 ataagggtat tgcctgtttc ggcattgaaa aatatcaaaa attgaactac attatcgcct
     5521 tttcaaaccc ggctgaaacc gacttttcaa acggcattca aataaaaact gccaaacacg
     5581 gacacaccat gaccacgact accgccccgc agcgtattcg ggaaatcccc tacaactata
     5641 cttcctacac cgaccgcgaa atcgtcatcc gcctgctggg cgacgaggcg tggcacattc
     5701 tacaggactt gcgcggacag cgcaaaaccg ggcgttcggc acggatgctg tttgaagtgt
     5761 tgggcgatat ttgggtggtc gtacgcaatc cgtatctggt cgatgatttg ctggagcatc
     5821 cgaaacgccg cgccgcgctg gtgcgggaaa tgcgccacag gttgaacgaa atccgcaaac
     5881 gccgcgacga taatcagcaa gcggatgtgt tgattgcggc ggcggaaaag gcagtggagc
     5941 gttttgacgg cagctttgac gaaacgcgcc aaaagcggcg gcagattttg gagcgtttga
     6001 gcaaaattac caagccgcac aatattatgt ttgacgggct ggcgcgggca acacacgtta
     6061 ccgatgcgac cgactggcgc gtcgaatatc cgtttgtcgt cgtcaatccc gacacggagg
     6121 cggaagtcgc gcctttggtg cgcgccttaa tcgagctgga cttagttatt atcccgcgcg
     6181 gcggcggtac gggttatacc ggcggcgcgg tacctttgga cgcaaacagc gcagtcatca
     6241 ataccgaaaa actcgacaag catcgcagcg tcgaatacgt tgagctggca ggcttgtacg
     6301 gcagacatcc gattatccgg tgcggcgcgg gcgtggttac gcggcgggtg gaagaaaccg
     6361 cgcatcaggc gggcttggtg ttcgccgtcg atccgacttc cgccgacgcg tcctgcgtgg
     6421 gtggtaatgt ggcgatgaat gcgggcggta aaaaagccgt attgtggggt actgccttgg
     6481 acaacctcgc ctactggaac atggttaacc ctcaaggcga atggctgcgt atcgaacgcg
     6541 tgcgccacaa tttcggcaaa atccacgacg aagaaactgc cgtgttcgac gtgcacacgc
     6601 tggattcaga cggcatcaac atcgttaaaa ccgaacgctt ggaaatcccc ggacacaaat
     6661 tccgcaaagt cggtttgggc aaagacgtta ccgacaaatt tttgagcgga ctgcccggcg
     6721 tgcagaaaga aggtacggac ggcatcatta ccggcgttgc cttcgtattg cacaaaatgc
     6781 cgaaatacac gcgcaccgtg tgtatggagt ttttcggcac ggccgccacc gccacgcctt
     6841 ctatcgtcga aatccgcgat tttctgctcg cacacgacag cgtgcggctg gcgggtttgg
     6901 agcatttgga ctggcgttat gtccgcgccg tcggctacgc caccaaagcg gcgggcaaag
     6961 gacgaccgaa aatggtcttg ctggcggacg tggtttcaga cgacgaagcc gccgtagagg
     7021 cagccgccga acacatctgc gaacttgccc gcgcccgtga cggcgaaggc tttatcgccg
     7081 tatcgcccga agcgcgcaaa accttttggc tcgaccgcag ccgcaccgcc gccatcgcca
     7141 aacacaccaa cgcctttaaa atcaacgaag acgtggtgat tccgctggag aggctgggcg
     7201 agtattcgga cggcatcgaa cgcatcaaca tcgagctttc catccaaaac aaactcaaac
     7261 tctgtgccgc tttggagcaa tatctttcgg gcaaactccc catcgacaaa atgggcaccg
     7321 acctgccgac cgccgaactg ttgggcgaac gcggcaaaca cgccctcgcc cacgttgccg
     7381 ccgtcaaagc gcgttgggac tggctgctcg cccatttgga tgcgccgctt gccgactaca
     7441 aatcacgcta cggtacagcc gtccacgccg cgcccgaagc caaagacgat gaaagctgct
     7501 tcaccgcctt ccgcgatttc cgcctgcgcg tgtctgtcaa agcagacgta atgaagccgc
     7561 ttgccgaaat cttcagcggc aaaaccgata ccaaaattat ccaaggcttg ggcaaaatcc
     7621 acgccaaaac cgtacgcagc cgcgtctttg tcgccctgca tatgcatgcc ggcgacggta
     7681 acgtccacac caatattccg gtcaactcag acgatgccga aatgcttcag acggcctacc
     7741 gttctgtgga acgcattatg aaaatcgccc gttcgctcgg cggcgtgatt tccggcgaac
     7801 acggcatcgg catcaccaag ctcgaattcc tcaccgatga agatttgcaa ccgttttgga
     7861 actacaaaaa ccaagtcgac cccaagcaca ccttcaaccg ccacaaactg atgaaaggtt
     7921 cggacttacg caacgcctac acgccgtctt tcgagctgtt gggcgcggaa tcgctgatta
     7981 tggaaaaatc agacctcggc acgattgccg attccgtcaa agactgcctg cgctgcggca
     8041 aatgtaaacc cgtctgctcc acccacgttc cgcgcgccaa cctgctgtac agcccgcgca
     8101 acaaaatcct cggcgtgggt ttgctgaccg aggccttctt atacgaagaa caaacccggc
     8161 gcggcgtttc cgtcaaacat tttgaagaac tgatggacat cggcgaccac tgcaccgtgt
     8221 gccaccgctg cgtcaaaccc tgccccgtca acatcgactt cggcgacgtt accgtagccg
     8281 tccgcaacta ccttgccgat tccggccaca aacgctttgc gcctgcggct tcaatgggta
     8341 tggcgttttt gaacgccacc ggtccgaaaa ccatcaaagc cctgcgcgcc gcgatgatac
     8401 agaccggctt ccccgcgcag aactttgcct acaaaatcgg caagcttctt ccgatcggca
     8461 cgaaaaagca aaaagccgaa cccaaggcaa ccgtcggcaa agccccgatt aaagaacagg
     8521 ttatccattt catcaaccgc ccactgccca aaagcgtacc cgccaaaaca ccgcgctcct
     8581 tattgggcat cgaagacggc aaaagcatcc ccatcatcca caaccccgcc gcgcccgaag
     8641 atgccgaagc cgtgttctac ttccccggct gcggttcgga gcgtttgttc agccaaatcg
     8701 gactcgccgt ccaagccatg ctctggcacg tcggcgtaca aaccgtcctg ccgcccggct
     8761 atatgtgttg cggctatccg caagacgctg gcggcaacaa ggcaaaagcc gaagaaatga
     8821 gcaccaacaa ccgcgtcgcc ttccaccgta tggcgaacac cctcaactac ctcgacatca
     8881 aaaccgtcgt cgtcagttgc ggcacttgtt acgaccagct tgaaaaatac cgcttcgagg
     8941 aaatcttccc cggctgccgc atcatcgaca tccacgaata cctgctcgaa aaaggcgtga
     9001 aactcgacgg cgtgaaaggt cagcaatacc tctaccacga cccctgccac accccgatta
     9061 aaaccatgaa cgccacccaa atggccagca gcctgatggg gcagaaagtc gttttaagcg
     9121 accgctgctg cggcgaatcc ggtatgttcg ccgtcaaacg ccccgacatc gccacacagg
     9181 tcaagttccg caaacaagag gaaatcgaga aaaacctcaa agagctgccg cagggcgaac
     9241 ccgtcaaaat gctgacctcc tgccccgcct gcctgcaagg actgagccgc tattccgacg
     9301 acaacaatat gcctgccgac tacatcgtca ttgaaatggc gaaacacatc ctcggcgaaa
     9361 actggctgga tgagtttgta aaaaaagcca acaacggcgg cgtagagaaa gtgttgctat
     9421 aacaacggac acggaaatgc cgtctgaacg ccgaaagcct tcagacggca ttgtttgaat
     9481 caaataccta cccgaatcat gattgacgga caaacaaccg aacccaaaca aaaaaccaga
     9541 atcataccag cccccatgcg ggggctggta gacgatgtga tgcgcgacct gctgacacgt
     9601 atcggcggct acgacgaatg cgtcagcgaa tttgtacgca ttacccatac cgtgcattca
     9661 cgatccatat ggttaaaata tgtccccgaa atcgccaacg gaaacaaaac gttttccggc
     9721 acgccctgca ccgtccaact tctgggcagc gatgcggaca atatggcggc aaacgcattg
     9781 gaagccgtcc gattcggcgc ggacaaaatc gatttgaact tcggctgccc cgcgcccacc
     9841 gtcaacaaac acaaaggcgg cgcaattctt ctgaaagagc cggaactgat attccacatc
     9901 gtcaaaacgc tgcgcggacg tttgcctgca catattccgc tgacggggaa aatgcggcta
     9961 ggctatgaag acaaaagccc tgctttggaa tgcgcctgtg cgattgccga agggggcgcg
    10021 tgcggactga ccgtgcacgc gcgcaccaaa gccgagggct acgaaccccg gcgcattggg
    10081 aatggataag gaaaatccac gataccgtca atattcccgt taccgccaac ggcgacgttt
    10141 tcagcctgca ggactatatc ggcatcaaaa caatcagcgg ctgcaacagc gtgatgctcg
    10201 gtcgcggcgc ggtcatccgc cccgatttgg cgcgccaaat caagcaatac gagaacggcg
    10261 gaccggtcaa agacacggat tttgccgaag tttccacatg gatagggcag tttttcgagc
    10321 tgtgcctgac aaaagaggca aacaacaaat acccgatcgc ccgtcttaag caatggctgg
    10381 gcatgatgaa aaaaacgttc gacccggcgc aaacgctgtt cgaccgcgtc cgaacggtta
    10441 aagatgcgga tgaagtgcgc cgaatcctga acgcatttga gcatgagatg gatgtttgat
    10501 gtctcccgcg cttcaaagca aatgccgtct gaaaaccttt cagacggcat ttgttatctt
    10561 tattgccgtt tttcccctgc tttcggtgta tttttgtttt tcagtttcgc ccccaaaccc
    10621 aaacgttttt cataatccga ttgcggggta tcgtcttcct gcataccgaa cgcgccggca
    10681 ttgacccaca gcgacagcag cgcgacgaca aaggcgcaaa aaccgattac ataccaaaac
    10741 attgcccctc caaatttgtt aaaatcatat caaatacagt gccgaattta tcacaagcgc
    10801 acgagcaaat aaatcttctg accgcagtat ttcattcatt taccttgaaa acaaaaaagg
    10861 gcacctgaaa attttgcgaa acttttcaga tgccctgctt tgccgaaaaa cgtatcagcc
    10921 gcagcaaccg ccttcgccgt gtccgccttt tttgtcgtta tcgacaatgg gcagttcgac
    10981 tttttcgttt ggtttttctg cattttcggg ccggtcgttg agtttgattt tcgcggtttc
    11041 gctcatttcc ttacctcatt tggaaaacgg tttgaaagat tcataaatat agcatgttta
    11101 atccgaatct gaaaagggaa gccgtatgcg aaatcccgtc tgaagcccaa tcagggcttc
    11161 agacgggatt tgctatcttt attgccgctt ttcttccgta tccggatttt tgtttggggc
    11221 tgaagcagat tggcagtcag attgcaatcg aagaatgaag gcaagccatc aaaaacaaag
    11281 ctatccgctt caccgccccg atatttagaa tttgtggcgc agaccaacgg aggcggcatt
    11341 aatttgagtg tagttgccga tgccggtatt tcgtttcagc caagcggcag acatgatggc
    11401 ggaagtgcgc ttggaaaaat cgtaatcgac accggcgatg atttgatcat agctggtatg
    11461 ttcgcgtttc tgactgcgtt cgacaaagtc gaaaccatgg gcatagctga tgcgcgggac
    11521 tgtattaccg aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt
    11581 gtcggcattt tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcccc
    11641 atagccgccc gtcaggcggt gtacctgatg gtttttcaat ggatcggtat cactcgctct
    11701 gccgagcaag aacaaaaaga aagcatcatg cccctcattg gcgtgtttcg catatttaag
    11761 ggcataattt ccgaaaaagc cgccattttt gtaattcaga ccggcataat acacatccga
    11821 ccccggcttg ccgacaacag ccggaactgg tttcatctga ttactttcca gcgtgaaagt
    11881 agcaggcgta taggcggact tgctgttttg actcggaaca aattgaatgc tgccgctgaa
    11941 accggaaaat ccgggggaat cgtaacgcac ggaaaccggc ataccgtcgt ggcgtttgaa
    12001 aatacccaat tgcgaagcca cattattatt gctgtcccaa ggatcaatgg ctttgctggc
    12061 atcgccaaac ggattcgcaa cgcgaccggc gagcgccgtg ccgaattcgc ctgccaagcc
    12121 gataaaggat tccctgttac cccaacgggt cgcgccgccg ccggcaacgg atacgtcttg
    12181 ctcgagctgc caaacagcct tcagcccgcc gcccaaatcc ccaccccctt aaagccgata
    12241 aacgagccga aatcactgac tttcgtcctg atgcggcttt tggccttagt aactgtattg
    12301 cccgtttgac cttctgaggg tggctcggtc aactgcagcc ggatgttcct gccttccaca
    12361 ccagctttga cttcgccgta caggctgaca tcggcaactg ccgcaaacgg cagtgcggac
    12421 aatacgaggg cggtaagttt ttttcgcata tcggcttcct tttgtaaatt tgctaaaaac
    12481 atcgggcaaa cacccaatca gtctccaatt accccccgaa aaaaaaacca tttttcggaa
    12541 caaatttcgg gtaaattttg gaatttttat tttgaaaaat aatatttcaa tataaaacaa
    12601 taaattaact tttttgaaaa tgttgctttc ccgcaacatg atttaaatac agaccttgtc
    12661 aaagccgatg cggcgcagac ccggatgccg tctcgttttt taaaacacca taaaaaaaca
    12721 cacgcggtgc tttaatttcc tgcgtgtgtc gagtgtatcg aaatcaagcc gaatcaaata
    12781 taccggactt cgataatatc gtattcgcgt acgccgcccg gtgcctgaac ttccgccgta
    12841 tccccttctt ccttgccgat cagggcgcgg gcaatcggag agccgacata gattttgccc
    12901 tgtttgatgt cggcttcgtc ttcgccgaca atttgatagg taacgtgttc ttccgtttcc
    12961 aaatcttcca gcgtaaccgt cgtaccgaac acgattttgc cttcggcgtg gatttcggcc
    13021 ggattgatga tgtgggcaac ggaaagtttg tgttccaact cggaaatgcg gccctcgata
    13081 aaaccttggc gttctttggc ggcttcgtat tcggcgtttt cggacaaatc gccgtgcgat
    13141 ctggcttcgg caatcgcttc gatcacttcg ggacgcgcca cgcttttgag ctgctgcaat
    13201 tcctgtttca gcaattccgc accgcgtacg gtcaggggga ttttttgcat cggtcttttt
    13261 ctccatattc cggcacaccg gtttgcggcg gcaagcatac cgcgtaccgt cttgttttgt
    13321 gcgtccggat attaaaataa aaatacaagc cgcccggaaa atcggcggct tgtctgtcgt
    13381 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgccggcaa
    13441 acgcgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
    13501 aatttaaaaa aaatcaattt tcggcataaa aaaccaaatt cacgtacttt aaaaccgaaa
    13561 atgccaaacc tgagattttc cagacggcat ttgcaccagt ataatgcagg ctgtttttat
    13621 ctttatattt acgttttgcc atgaccgaat ccgtccgcct ccccgccgcc tcgctcaaac
    13681 cttccaccgt cgccctgccc ggctccaaaa gcatcagcaa ccgcaccctg ctgctcgccg
    13741 ccctgtccga caatgtttgc gaaatccatt ccctgctcaa atccgacgat accgaccgta
    13801 tgctcgaagc actcgataaa ctcggcgttc aaatcgaaca tcttgccgaa ggccgtctga
    13861 aagtacacgg cacgggcgga cgcttcccca accgctctgc cgatttgttt ttgggcaacg
    13921 cgggcacggc gttccgtccg ctgaccgccg cgctggccgt tttgggcggc gattatcatc
    13981 tgcacggcgt gcctcgtatg cacgaacgcc ccatcggcga tttggtcgat gcgttgcgga
    14041 ttgccggcgc cgatgtcgaa tatctcggca acgaacacta tccgccgctt catgtcggcg
    14101 aactccaaaa caacggcgaa cgcgtgattc cgattaaagg caatgtgtcc agccagtttc
    14161 tgaccgccct tttgatggcg ttgccgctga ccgggcaggc gtttgaaatc cgtatggtcg
    14221 gcgagttgat ttccaagccc tatatcgaca ttaccttgaa actgatggcg caattcggag
    14281 tacaggttgc caatgaaggc taccgcgttt tcaaaatccc tgccgatgcg cactatcacg
    14341 cgcccgaaca cttgcacgtc gaaggcgatg cctccggcgc gtcctacttc ctcgcagccg
    14401 gtttgattgc cgccacgccc gtccgcgtta ccggcatcgg cgcaaacagc atacagggcg
    14461 atgtcgcctt tgcccgcgaa ctggaaaaaa tcggtgcgga cgtggtttgg ggcgaaaact
    14521 tcgtcgaagt ctcgcgcccg aaaggacgcg ccgtccaagc ctttgatttg gatgcgaacc
    14581 atatccccga tgccgcgatg accctcgcca tcgtcgcgct tgccacaagg caaacctgca
    14641 cgcttcgcaa catcggttcg tggcgcgtta aggaaaccga ccgcatcgcc gcaatggcga
    14701 acgagttgcg caaactcggt gcaaaagtcg tcgaagaagc cgaagcgatt cacattaccc
    14761 cgcccgaaac gccgacaccc gatgccgtca tcgacactta cgacgaccac cgcatggcga
    14821 tgtgtttctc gctgatttcg ctgttgggag tacccgtcgt catcaacgac ccgaaatgca
    14881 cccataaaac cttcccgact tattttgaag tgttctcatc actgaccgaa acagcggaat
    14941 aaggcggaaa aatgccgtct gaaacccgaa aacagggctt cagacggcat tttgccgcga
    15001 ttccggcgcg gcgggcggct cattctgtaa aaaacgtatg cgctccaagg tagtttttgg
    15061 cgtaaaacgg tgtggagagt ttttcggttt tgatggtttt gccgctgccg ggggcatgga
    15121 tgaattcgcc gttgccgatg tagagtccga cgtgtgagta gcggtgtgcg ccgccggtgt
    15181 tgaagaatac gaggtcgccg gccttgaggc ggctgtcggg gattttgcgg cttgccgccg
    15241 ccatgtcgcg ggcggtgcgc ggcagcttga cgttgagggc gtttttgtaa acgaattgaa
    15301 tcatgccgct gcagtcgaag ccggttgcgg tgctgctgcc gccccatttg tagggcgtgc
    15361 cgatgagtcc gaggctgtgg agcatgagtt cctgcgagcc ttgtgtgcgg ccgatgtggc
    15421 tgatgcggac ggcttggatt tgccggactg tctgtttggg tttcggttgg cggtgtttgc
    15481 cggaggtcgt gccgcatgag gcgaggagca gtgcgccgag acagaggaaa agggttttgt
    15541 cgggggaaaa catggttttt tcctttgcgg gttcggatat gccgtctgaa ggtgtttcag
    15601 acggcatttc tataataaac cttcgatggg cagcagggat aggatttttg cggcgatgcg
    15661 tttccaaagt ttggcttcgg gttcgttcgg gtaggttttt cgggtggcgg gatcgtgcca
    15721 ttgcaggcgg ttgtgtttgt cgagggtaac gcggtaggcg tattcgggtg tggtatcggc
    15781 gagggtgcgc tccatctgtt ctgcgatttt ggggctttcg atgacgacgc ccatttcggt
    15841 attgagccgt gcggaacggg ggtcgaggtt gaatgagccg atgaagatgc gtttgccgtc
    15901 cacaatgaag gttttggcat gcaggctggt tacggagctg ccggtcaggc ctttgtcttt
    15961 tgtggcgggg acggcatggt tgggttgcag ctcgtagagt ttgatgccgg ctttgagcag
    16021 cggttttcgg tatttgacgt agccggaatg gacggcggca acgtcggtcg cctgtagcga
    16081 gttggtcagg acggtaacgt ctatgccgtc ctgcaccagt tttgccagtg cgtctgtgcc
    16141 ggattttgta gggacgaaat agggtgaaac cagatagacg cttttttcgg gctgtttgag
    16201 cgcgtcttgc agcctcccgg caatcggcgg tttgcggcgg tcgcggtcga gtccttttgc
    16261 agggttgtcg ctgatcaggc gggtttggac gctctgccag tcgatgcgtc ccgtctgtat
    16321 tttttggtag aggggcgact gttcgacggt ttcgcggtag cgcaggagcg cgtgtctgga
    16381 tgtttcgtcg ttgtatccga gtgcttgaag acccttgccg atgttgccgc tgcggatgat
    16441 gcgcgtggcg ttgtgggcgg aatggcttgc ccagtagcgg tcgaagtcgt gcgatacttc
    16501 gccgacgacg ctgccggtgg cgaggatgtc caggtcggcg aaaacggtgt cctcaccgac
    16561 tttgaagtat tcgtcgccga tattgcgtcc gccgagtatg gtggcgcggt tgtcggcggt
    16621 aaaggatttg ttgtgcatgc ggcggttgag gcgggggaag tcggtcaggt agccgagtgc
    16681 gcgccatttg cgtaggacga aggggttgaa caggcgcact tcgatattgg gatggctgtc
    16741 gagggcgagc aggagatcgt ccaacccgcg cgtgttgttg tcgtccaaca gcaggcgtac
    16801 gcgcacgccg cgttctgcgg caaggtacat gaggttgaac agcagcctgc cggaaatgtc
    16861 gttgcgccaa atgtagtatt gcaaatcgag gctgtgttcg gcagattcga taagggcggc
    16921 gcgggcggca aaggcttcgt gggggtcgtc gagcaggtag atgtcggata gcccgttgtt
    16981 atgaggggtg tgccggattt gcaggatgtt gtccaggagg acaggtttgg aagtattgaa
    17041 atgacggctt tccgtccgtt cttccagtgg gggcaaccat gaagaacatg aacagagaag
    17101 gaggcataaa agggaaatga ggctgcgtgt tttcatcggg gatatagttt cagacggcat
    17161 tgcctgtatt ttggggtctg tatgcattga aatgaagcat cataatctaa aggttggaat
    17221 gggtaaaagt tttgtcggga tgcggggtta aggccgcgtg cctcttgtca ggtttgcacc
    17281 gtctgaaacg tgcagacgct taaaaaccaa cgacgaggca agggtgatgc cgccgatact
    17341 gagcagggtc atacggaagg cgaaatgcag gcctgaagaa gccggtatca gaaatgtcca
    17401 gtttttaagg attaatgcgc cggcaacaat gcccacgctg atggcaagct gttggttgac
    17461 cgccatcagg ctgttgccgc tgccggtctg ttgcgggcgc aaatcggcga gggtcagtgt
    17521 gttcatggca gaaaactgta gggagttgca tgcgccgatc gccagcgaga ggaaaatcca
    17581 aatccacagc ggcgagtttc cgtcaggcag ggcgagcagc atgatgaagg cggcaagcag
    17641 cttggtgttc caaagcagta ccgtgcggta gccgaaacgt ttcatgagcg gtgcaatcag
    17701 cggtttgacc agcagcgaag acagggcgac gggtgcgacc agccagcccg acaggcttgc
    17761 gccgaagccg aaagcgactt gaaacatcag gggcatcaga aaaggaatcg agctgatgcc
    17821 gagacggctg aacagattgc ccgccagtcc caaacggaag gtgcgtatca gaaacaggtc
    17881 ggcggaataa atcggtttgg acgcggtttt catatgtcgg aaataacggc gtgcaaacag
    17941 cagtccgccg cacagcggta acagtgcaaa atacggaggc agcgcgtgcg acaggctttc
    18001 tgccgcaagc agcagcaggc aggcggcaac ggaaaaggtc agatagcctt tgaaatccag
    18061 agcggtatcg ttgcctttga catcgggcat gatgttgcgt cccaatatga aacccagcag
    18121 accgatgggc aggttgagca ggaaaatcca gtgccacgaa gcgtattcga ccagataacc
    18181 gcccgccaga ggtcccaacg cagggccgat cagtgcaggc atgaccgcat aattgatggc
    18241 gttgagcagc ttcgattttt catatacacg caagatggtc agacgcggta tcggaaccag
    18301 catcgaaccg ccgatcccct gaacgacacg ggaaagcgtc aattcaaaca gcgaacccga
    18361 tgcggcgcac aatgccgatc cgagcataaa aacggcaatc gaaccgaaaa agactttttt
    18421 cgttccgaac ttgtccgcca aataactgct caaaggaatc agcagggcaa ccgtcagcgt
    18481 ataggcaacg actgcctgtt gcatatccgg aggcgattcg ttcaggtcgg cggcaatttc
    18541 gggcagtgcg gtatttaaaa tggtcgcatc caacatctgc ataaaaatgg caattgccag
    18601 cagaagcggc agccaagggg atggtgcgcg ggcggacagg gtgttttttt ccatagggtg
    18661 attgtacccc atccttgtgc cgttattgtt ttcagatgct gtctgaatgc cggcagagtc
    18721 ggcatcctga atgttcacaa gcaaacgaat cggcattgca ttgtaatgat aattattatc
    18781 gaaaatcatc agagtaaggt acagtaagcg ttatggggca gtttgtaaga aaaaccggat
    18841 tattttttaa aattagactt gacccgcaac ggtcaattac ttaaagtaaa cgcttacctt
    18901 tctacagaaa aaacgggttt cccgttatca aaaaatatga gcgcaaccat tcccccaaaa
    18961 atcatccgat acgacagcaa tccgacagat gtctattttt tcggcacttg cgtccttgat
    19021 ctttttatgc ccgaagcagg catggatgcc attgccctaa tcgagcagca gggcatacgc
    19081 gtccatttcc cgatggcgca aagctgctgc ggtcagcctg cctattcatc cggccatccg
    19141 accgaagcct tcgatgtcgc caaagcgcaa ctcgaccttt tccccgaaaa ctggccgatt
    19201 gtcgtaccgt ccggctcatg cggcggcatg atgaaacatc actggcctac cctgtttaaa
    19261 aatacagaat acgaatccaa agcggttgat tgtgccaacc gtatcattga gtttactcat
    19321 ttcttgcttg ccatcggcta caagcctgaa gacaaaggcg agccagtcaa agtcgccgtt
    19381 cacacttcct gtgccgcccg ccgagaaatg aatgtccatc tttcaggctg gcagttgatt
    19441 gacggtatgg aaaacgtcga acgcatcgtc cacgaccacg aaagcgagtg ttgcggcttc
    19501 ggcggcacat tctccgtcaa acaagccgat atttccggcg cgatggtaac cgacaaagtt
    19561 gccgcgctga aagaaaccgg cgcaaccgaa atcatcagcg cggactgcgg ctgtatgatg
    19621 aacatcggag gcaaaatcgc caaggacgag ccggatatgc cgcgtccgaa gcatatcgca
    19681 tccttcttgt tggaacgcac cggaggcaaa gtatgagcgc gcgcgaaaat attttggcaa
    19741 aactgaaaaa agccggcgca ttgccgatgg aagagcctgc ggtttttgat tattaccgcg
    19801 aaaagggcgt ttcttgggac agcgaagccg agcgtctgaa acattgggct gccgccatgc
    19861 gcgcggtcaa aaccgaaatc tattgggtga cgaaaagcaa ttggatgcag gttttccgcg
    19921 aagcggcaga aggcaagggt ttaaaaaaca tcctgctgcc cttggcgacc gaacacggac
    19981 aaattgcccg tgccgcattg gcgggcagca atatcgaccc gattgccttc gagcgcgaaa
    20041 tcgatacttg gaaaaccgag tttttcacga acatcgatgc aggcttcagc ggcgcgcaat
    20101 gcggcatcgc ccgcaccggc acgctgatgc tgttttccag ccctgaagaa ccgcgtactt
    20161 tgagcctcgt tccgcccgta catttctgcc tgttcgatac gtccaagatg tacaacgagt
    20221 ttcacaacgc cgtcgaaggc gaaaaactgg tggaaaacgg tatgccgacc aatgtattcc
    20281 tgatttccgg cccgtccaaa accgcagaca tccagttgac gctcgcttac ggtgcgcacg
    20341 gtccgcgtga tttggtcatc ctcgccattt tgcccgacca catttcccct gccgatttgg
    20401 aggaaaacgc atgactacgc aaaccatcaa gttccacatg aagccggaaa ctttcaaaca
    20461 aaacgccgca atttcccttc aagacaagcc cttgcgtaaa agcctgcgta ccgcgatgga
    20521 tatgctgatg accaagcgca aagccgtttt gaccgacgaa gaagagttgc aaagcctgcg
    20581 cgatttgtgc gaacacatcc gtcagcgttc cctctccaaa ttacccgcgc tgctggagca
    20641 gttggaagaa aacctgacta agttgggcgt gaaagtacac tgggcggaaa cccctgccga
    20701 agcctgccaa attatccacg acatcatcac agccaaaaac ggcaagctga tggtcaaagg
    20761 caaatccatg gtcagcgaag aaatcgagct gaaccattat cttgaagcaa aaggcattaa
    20821 ggcggtggaa agcgacttgg gcgagtttat cgtccaaatg gcaggcgaaa aaccgaccca
    20881 tatcgtgatg cccgcaatcc ataaaaccaa agaacaggtc agcgaactgt tccaccaaaa
    20941 cctcggcaca ccgcttacag acgacgtgga ccaactgacc ggattcgccc gcaaagccct
    21001 gcgcgatatt tacagcactg ccgatgtcgg cttgagcggc gtaaacttcg ccgttgccga
    21061 aacgggcacg ctgtgtctgg tcgaaaacga aggcaacggc cgcttgagta ctaccgttcc
    21121 gcccgtacat atcgtcatta cgggtattga aaaagttgtc gccaagctgt ctgatgttcc
    21181 gcctttgtac agtctgctgc cgcgttccgc catcggtcag aacattacca cttatttcaa
    21241 tatgattacc ggcccgcgcc gcagtgaaga attagacggt ccgcaggaaa tgcacttggt
    21301 tttgctcgac aacggccgca gccaagctta tgccgaagac caaatgcgcc gcaccctgca
    21361 atgtatccgt tgcggcgcgt gtatgaacca ttgcccggtt tatacacgca tcggcggcgc
    21421 ggcatacggc acaacctatc ccggtccgat tggcgagatt atttccccgc acctgttggg
    21481 tcttgatgcc acccgcgacc tgccgaccgc ctgcacgatg tgcggcgcgt gcgtggaagt
    21541 ttgtccggta cgcatcccga ttaccgaaca aatgcaacgt ttgcgcgttg aagcacaacg
    21601 ttcgccgacc gaaaccgtgc cgcaccccat ccgagggcaa ggcgcatcgc ataccttcgg
    21661 cgaacaaatg gcgtggcgca cattcaacgg tattttcagc ggtagcaaaa cctaccgcgc
    21721 cttcggttgg gcagccacca agttccgcaa tctgaccccg cgcaaacagt tgggttggac
    21781 gcaaaaccgc gtaccgatga aaccggcgaa gaaaaccctg cacgaactga tggcggaaaa
    21841 aatgcgccaa aaagaacagg cataaaaagt tgttcgcaaa aataccgtct gaaacccgaa
    21901 aacagggctt cagacggcct gaaaagcagc ctgaacgcgc aaaaataaat aatgaaacgg
    21961 aatgctaaga cgctttcagg cagcctgaca ttaaggctgc ttaaaagcgt cttttaatcg
    22021 tgccacggat tgaacaccgc gacatcggcc gcaaaaaatg agccggtatc acgcgtagca
    22081 actgtcaaac tgtgctgttt tgcagtggcg gcaatatagc cgtctgcggc agcaatctct
    22141 ttgccatgtg ttttggcata ggaacgaatt tgcgcataga ttgcggcaac cggttcatca
    22201 aaaggcagaa tccgccccgc aaataaaggc aaaatggatt gttccaaacg ttcgtgcagc
    22261 acattctttt tcttgccatt gagcaacaac gccacaccca aacgcaattc tgcaacagta
    22321 atggcagaca aatacacatc ttccaatatc aaactatcca accatgccac cacacgttca
    22381 ttgggttgtg ggcgcaaagg ttcggaaatc acattcgtgt ccagcaaaat catcacaaag
    22441 aaacctcgtt atcagtatta cgaccgcgta cgtcttccag ctcaacacct ccgatttcct
    22501 gccctattga tgccaacatt gaccccaaac gtacagtttg ttgtgctttg gcgatgttat
    22561 ccaaaattaa gcggatttct gcttcggtac tgcgccctgc ggctcgcgca cggaatttga
    22621 ttgcgttgtg cgtggcctcg gataaatttc taatcacaac agaagccatt gcctttcctt
    22681 cctgaaaaaa gtttttcaaa tgctatcaaa ataaaaaaaa atgatagcaa tctatttttt
    22741 aacgcctgcg gatgcctgaa aattcaggtg gccgcaggcg tttttttaca tctaaaaaaa
    22801 cgtctcccct ccctctttct gataaataaa aaacagtcgc tc
//
LOCUS       DAXFHF010000033        22096 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXFHF010000033 DAXFHF010000000
VERSION     DAXFHF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22096
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.033"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            96..917
                     /locus_tag="ACFIRM_001155"
     CDS             96..917
                     /locus_tag="ACFIRM_001155"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC3028795.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1003..1665)
                     /locus_tag="ACFIRM_001156"
     CDS             complement(1003..1665)
                     /locus_tag="ACFIRM_001156"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3028796.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1703..2530)
                     /locus_tag="ACFIRM_001157"
     CDS             complement(1703..2530)
                     /locus_tag="ACFIRM_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC3028797.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTTEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2913..3536
                     /gene="lolA"
                     /locus_tag="ACFIRM_001158"
     CDS             2913..3536
                     /gene="lolA"
                     /locus_tag="ACFIRM_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC3028798.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3809..4948
                     /locus_tag="ACFIRM_001159"
     CDS             3809..4948
                     /locus_tag="ACFIRM_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3028799.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5043..6047
                     /gene="pglE"
                     /locus_tag="ACFIRM_001160"
     CDS             5043..6047
                     /gene="pglE"
                     /locus_tag="ACFIRM_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HFC3028800.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKISNTKHQTPNTKHQTPNTKYIYQDNQGLSEARNTGIKNSNGKYIAFI
                     DSDDFINCQVLLDFLGKDDSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEV
                     LKGLCRFRKFPGSAWNKIIKRELIIREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDG
                     CYYYYRQGRKDSITGTVSEKGIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLF
                     IMTSKNIACDSDIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI
                     "
     gene            6088..6618
                     /locus_tag="ACFIRM_001161"
     CDS             6088..6618
                     /locus_tag="ACFIRM_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC3028801.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(6786..8381)
                     /locus_tag="ACFIRM_001162"
     CDS             complement(6786..8381)
                     /locus_tag="ACFIRM_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC3028802.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(8488..8883)
                     /gene="hisI"
                     /locus_tag="ACFIRM_001163"
     CDS             complement(8488..8883)
                     /gene="hisI"
                     /locus_tag="ACFIRM_001163"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC3028803.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(8914..9681)
                     /gene="hisF"
                     /locus_tag="ACFIRM_001164"
     CDS             complement(8914..9681)
                     /gene="hisF"
                     /locus_tag="ACFIRM_001164"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC3028804.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(9694..10431)
                     /gene="hisA"
                     /locus_tag="ACFIRM_001165"
     CDS             complement(9694..10431)
                     /gene="hisA"
                     /locus_tag="ACFIRM_001165"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC3028805.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDTTVKLAQSVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(10464..11102)
                     /gene="hisH"
                     /locus_tag="ACFIRM_001166"
     CDS             complement(10464..11102)
                     /gene="hisH"
                     /locus_tag="ACFIRM_001166"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC3028806.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(11232..12734)
                     /gene="pta"
                     /locus_tag="ACFIRM_001167"
     CDS             complement(11232..12734)
                     /gene="pta"
                     /locus_tag="ACFIRM_001167"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC3028807.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLTQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(12919..13977)
                     /gene="fbpC"
                     /locus_tag="ACFIRM_001168"
     CDS             complement(12919..13977)
                     /gene="fbpC"
                     /locus_tag="ACFIRM_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC3028808.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(13998..15515)
                     /gene="fbpB"
                     /locus_tag="ACFIRM_001169"
     CDS             complement(13998..15515)
                     /gene="fbpB"
                     /locus_tag="ACFIRM_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC3028809.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(15583..16578)
                     /gene="fbpA"
                     /locus_tag="ACFIRM_001170"
     CDS             complement(15583..16578)
                     /gene="fbpA"
                     /locus_tag="ACFIRM_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC3028810.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(16830..17310)
                     /locus_tag="ACFIRM_001171"
                     /pseudo
     CDS             complement(16830..17310)
                     /locus_tag="ACFIRM_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(17368..18744)
                     /gene="argH"
                     /locus_tag="ACFIRM_001172"
     CDS             complement(17368..18744)
                     /gene="argH"
                     /locus_tag="ACFIRM_001172"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC3028811.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIELRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(18763..19632)
                     /gene="galU"
                     /locus_tag="ACFIRM_001173"
     CDS             complement(18763..19632)
                     /gene="galU"
                     /locus_tag="ACFIRM_001173"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC3028812.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHSVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(19660..20259)
                     /gene="rdgB"
                     /locus_tag="ACFIRM_001174"
     CDS             complement(19660..20259)
                     /gene="rdgB"
                     /locus_tag="ACFIRM_001174"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC3028813.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHHTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(20252..20485)
                     /locus_tag="ACFIRM_001175"
     CDS             complement(20252..20485)
                     /locus_tag="ACFIRM_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC3028814.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(20593..21126)
                     /locus_tag="ACFIRM_001176"
     CDS             complement(20593..21126)
                     /locus_tag="ACFIRM_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC3028815.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(21228..21686)
                     /gene="nudB"
                     /locus_tag="ACFIRM_001177"
     CDS             complement(21228..21686)
                     /gene="nudB"
                     /locus_tag="ACFIRM_001177"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC3028816.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            21897..21973
                     /locus_tag="ACFIRM_001178"
     tRNA            21897..21973
                     /locus_tag="ACFIRM_001178"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21931..21933,aa:Pro,seq:ggg)
BASE COUNT         4679 a         5452 c         6633 g         5332 t
ORIGIN      
        1 gggtgtaatc cgaggtaggg gcggctgggg tgcttctccc ttgtctgccg ctgctgttat
       61 gatgatattt ttattctgta tttaaggagg gggtgatgag cagtccgcgc caagtgtttt
      121 atatttccga ccgcaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
      181 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
      241 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
      301 ttgtcagtgt ggttgatgac gaaatccgcc ggattattaa aggggcggat gcttttcaga
      361 ttaatttctt tgagactttt ttgggactgt tggagaagga actcaatacc gaagcaacgg
      421 tatccgggca ggggcatcac agtatcggca atacgaagcg ttacgatgcg cgtatggagg
      481 cggtcaattt ttctttgaac catgatgacg gggtcagcga taagaacctt caggaggcgg
      541 atgtgattct gatgggcgtg tcccgttcgg gcaaaacgcc gacctgcctg tatctggcgt
      601 tgcaatacgg tatccgtgcg gcaaactatc cgctgattcc cgacgatttg gaatcggccg
      661 atctgccgcg tatggtcaag ccgtataaag acaaactgtt cgggttgact atccagccgg
      721 aacgtttgca ggccatccgt caggaacgcc gcccgaattc ggcttatgcg cgcatcgaca
      781 cctgccgcag cgaagtggcg gacgcgcaga gtatgttcag acggcatggc attccgtttg
      841 cgaatacgac ggacaagtcg gttgaggaac tggcggtaca catccttcag gcgtgcaagc
      901 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg tggaaatgcc gtctgaaatc
      961 aggtttcaga cggcagtttt atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     1021 cagacattcg agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     1081 atcgtcttca tcggaaatat agccccattc ggcgaggacg gttttcatac cggcgttgcg
     1141 tccggcttgg atgtcgcgtt ccgcgtcgcc gacgtagagg gtgtgttgcg ggtcggcgtg
     1201 gatttttccg caggcgtgca gcatgggttt gatgctgggt ttgggttcgc cgcaggtgtc
     1261 gccgctgacg acggtggcgg gcggaacggc gaatccgagt ttgggaacga gtttgtcggt
     1321 aaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgt cgagttcggc
     1381 gatgagttcg ttcacgccgt cgaagagagt ggtgtcttgg gcgtagcggc tgtcgtattc
     1441 ctccagatat tcggtgcgcc atgcggtata gtcggggtgt tcgggcgtga tgttcgcgcc
     1501 gagtttgagc aatcctgccg cgccgtggct ggcttgggtg cggatttcgt ccatgctttt
     1561 ttcgggcagt ccgtggcggg cgagttgggt gttgagtgcg ccgccgaggt ctagggcggt
     1621 gtcggcgagc gtgccgtcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttttt
     1681 ataaagtgcg ggacgaaagg tttcagacgg catgtttatt ttgtttcaaa ccctgctcga
     1741 aatcttccaa catatccaat tcgaagcggc tgaagcccgc tttttcccgc gcttcgatgt
     1801 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggcga
     1861 caggttccaa accgcgttcg cggcagaggt gttgatacca ccggttgccg atggcgacgt
     1921 gtcccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa tcgccgcgct
     1981 gctccacctt cgcgcgtatc cccggcgtaa cgtccagtcc gcgcgcttcc aacacgcgcg
     2041 gcaccaacgc catacgcaac aaaggatcgt aggcggtttt gtatgccata tcccacaaat
     2101 ggttgtgcgc ctcgaaactg ccgtaatcga agccgaaagc gcgcaatctg tcgcgcacca
     2161 ggcggaagtg gtacacctcc tccttcgcca ctttcaccca gtcgcggaca aactgaaacg
     2221 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     2281 gcgtaatcgc gtgcagcata gccgcatagc cttcggttgt gttcattttg cgcggggtca
     2341 gttgcgaggg cgcgaccaaa acaggcgtgt ccggccgtcc cgcgcggggg aagtccgccg
     2401 gcggtgcgtt tgcctccgcc ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca
     2461 gccgtccttt ttcatcgggg tcgcccgaaa gcagggcgcg ttccggcaaa gcataaatat
     2521 cgggtttcat ctcaagtccg ccgtgttcgg aaaacgaata ttatagcgtt taaaaaaaac
     2581 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     2641 gattaacaaa aaccagtacg gcgttgcccc gccttgccgt actatgttgt actgtctgcg
     2701 gcttcgtcgc cttgtcctga tttttgttaa tccactataa cgcggcacac aattgaaagg
     2761 gcagcgtggc gcgcctgctt tttccgagcg gtcaaaaaaa tcagccctcg gaaaacgcgg
     2821 tttgcaaaat gcaaaccgcc cgtaacgccg cccgtatgat tgttttgccg cgccgatact
     2881 ttgcgccaca cccatcccga caaggaaaaa taatgatgaa accgcacaac ctgttccaat
     2941 tcctcgccgt ttgctccctg accgtcgccg tcgcttccgc acaggcgggc gcggtggacg
     3001 cgctcaagca attcaacaac gatgccgacg gtatcagcgg cagcttcacc caaaccgtcc
     3061 aaagcaaaaa gaaaacccaa accgcgcacg gcacgttcaa aatcctgcgc ccgggcctct
     3121 tcaaatggga atacactttg ccctacaaac agactattgt cggcgacggt caaaccgttt
     3181 ggctctacga tgttgatttg gcacaagtga ccaagtcgtc ccaagaccag gccatcggcg
     3241 gcagccccgc cgccatcctg tcgaacaaaa ccgccctcga aagcagttac acgctgaaag
     3301 aggacggttc gtccaacggc atcgattatg tgcgggcaac gcccaaacgc aacaacgccg
     3361 gctaccaata catccgcatc ggcttcaaag gcggcaacct cgccgccatg cagcttaaag
     3421 acagcttcgg caaccaaacc tccatcagtt tcggcggttt gaataccaat ccccaactct
     3481 cgcgcggcgc gttcaagttt accccgccca aaggcgtgga cgtgttgagc aactgatgcc
     3541 ggccgccccg atgccgtctg aacgccgccg aggcttcaga cggcattttc acgcaggcgg
     3601 aacaatgtcc cgcattatcg gccgatcggg caacggaacg gcaaatccgt gaaaattaac
     3661 ggttgcgccc cggctgtttt tgccgtttaa tgcaaacctt gctgcaccaa gggccaagaa
     3721 agccgaccgg ccgcccccac agcttccgat gcaggcggcc cgtccgtccc tgcaatgttt
     3781 tttatttttg aacgaaaggt cgaaaaccat gaaaaaaaca ctggtggcgg cggcaatcct
     3841 gagccttgcc ttgactgcgt gcggcggcgg aagcgatacc gccgcccaaa ccccctccgc
     3901 caagcccgaa gccgaacaat cgggcaaact caacatctac aactggtcgg attatgtcga
     3961 tcccgaaacc gtcgccgcct ttgaaaaaga aaccggcatt aagatgcgtt ccgactatta
     4021 cgacagcaac gaaacactgg aggcaaaagt cctgaccggc aaatccggct acgacctgac
     4081 cgcgccgtcc atcgccaacg tcggccggca aatcaaagcg ggcgcgtatc agaaaatcga
     4141 caaggcgcaa atcccccatt acggcaacat cgataaagat ttgctgaaaa tgatggaagc
     4201 cgtcgatccg ggcaacgaat acgccgtccc ctatttctgg ggcatcaaca ccttggcaat
     4261 caacacgcgg caggtgcaaa aggcattggg tacggacaag ctgcccgaaa acgagtggga
     4321 tttggtgttc aaacccgaat acaccgccaa actcaaatcc tgcggcatca gctatttcga
     4381 cagcgcaatc gaacagattc ccttggcgtt gcactatttg ggcaaagacc ccaacagtga
     4441 aaatcccgaa gacatcaaag ccgccgtcga tatgatgaaa gccgtccggg gcgacgtgaa
     4501 acgcttcagc tcttccggct atatcgacga tatggcggcg ggcaacctgt gtgccgccat
     4561 cggttacggc ggcgatttga acattgccaa aacccgtgcc gaagaagcgg caaacggcgt
     4621 ggaaatcaaa gtattgaccc cgaaaaccgg cgtgggcgtg tgggtggatt cctttatgat
     4681 tccgcgcgac gcgcaaaacg ttgccaacgc ccaccgctat atcgattaca cgctccggcc
     4741 cgaggtggcg gcgaaaaacg gcagcttcgt tacctacgcg cccgccagcc gccccgcgcg
     4801 cgagctgatg gatgaaaaat acacctccga cgcatcgatt ttcccgacca aagaactgat
     4861 ggaaaaaagt ttcatcgtat cacccaaatc cgcagaatcc gtcaaactgg gcgtgaagct
     4921 gtggcaaggg ctcaaagcgg gcaaataacc ggaatccctg ccgtctgaaa cctttcgggc
     4981 ggcaggaaac ggcgtgtccg cattaagtca ggattaggat tatttagaaa gatgagatgg
     5041 atatgaattt aagtattgtc gttcctattt ataatgtcga aagttatttg gaagcgtgtt
     5101 taaattctat agaacctata ttaagtaatg aaaatgtcga acttattctt gtgaatgacg
     5161 ggtcaaaaga cggaagtgaa gatatatgtt ataaatatat agataaaata tcaaacacca
     5221 aacaccaaac accaaacacc aaacaccaaa caccaaatac caaatatata tatcaggata
     5281 accaaggatt gtcggaggcg agaaataccg gaataaaaaa ttcaaatgga aaatatatag
     5341 catttattga ttcggatgat tttattaatt gtcaggtttt gctggatttt cttggtaaag
     5401 atgatagtga tatgccggat gtggtttttt taaatgcagt taaatatgat aagggaagag
     5461 tttcatattt tggcgaagat tatcagcctg aaaaaatatt aaatcaatcc aaagtcgaag
     5521 ttttgaaagg attgtgccga tttagaaaat ttccgggttc ggcgtggaat aagattataa
     5581 aaagagaatt gattattaga gaaaaactgt tttttgaaaa gggaatttat tctgaagata
     5641 ttgaatggtc aatgaggttg tttaatgcgg caacaacttt ttcttatttg gacggttgtt
     5701 attactatta tcggcaggga agaaaagatt ctattacggg aactgtttcg gaaaaaggta
     5761 taaagtcatt attatatatt ttggaaaaaa atgcggaaat ggaatttaat agggatatat
     5821 cgagttatct ttattctttt ctttcctacg aatatctcgt tttgcttttt ataatgacga
     5881 gtaaaaatat agcgtgtgat tctgatataa aaagaagggc gtatcattta aggtttatgc
     5941 tgttaaagtc caataagctg atatataagc tgatattccc gataatcaca ttattcgggg
     6001 tcgatattac aggcaggatt ttaaaagcaa tcagggggaa tatttaataa atcctttaac
     6061 aatatatacc ttaccgaagg aggaaaaatg aacgcaatca gaactttcca aaaccgcacg
     6121 cccgaaatcc acgaaacctg catgatagac gaggcgtgcg tcgtcatcgg cgaagtctcg
     6181 cttgccgaag atgtttccgt gtggccgtgc gccgtgttgc gcggcgatgt gaacagcatc
     6241 accgtcggcg cgcgcagcaa tatacaggac ggcagcgtcc tgcacgtttc ccacaaaacc
     6301 gccgccaaac ccgaagggtc gccgctggtt atcggcgaag acgttaccgt ggggcacaaa
     6361 gtgatgctgc acggctgccg aatcggtaac cgcgtcttgg tcggtatggg cagcaccgtt
     6421 ttggacgatg ccgttattga agatgacgtg atgatcggcg cgggcagcct cgttcctccg
     6481 cgcaaacgct tggagggcgg ttatctttat gtcggttcgc cggtcaggca ggtgcgcgtg
     6541 ctgaccgatg aggaaaaagc ctttttgaag tattccgccg cacattatgt gaagctgtcg
     6601 aaacagtacg ggatgtgaaa tcacatcggc gttcttgcgt cagtcccaaa ttcacgcggt
     6661 tgggatgccc gataacggta tccgatgcgc cttgattttg accggctgcg tttgagttgc
     6721 aggcaaaaat gccgtctgaa agcctttttt tcggcttcgg acggcatttt attgccgatt
     6781 tctttttaga gtttgaccga atgttcgcgc gtttcgtgga acacgatgtc cggccaacgt
     6841 tcttgcgtca accccaaatt cacgcggttg ggggcgaggt aggcgaggtt gccgcctgcg
     6901 tcgatggcga ggttgcctgc gttggctttt tcaaattccg ccagtttttt cttgtcgtcg
     6961 cacgataccc agcgcgccga ccagatggat gcgctgtcga acacggcttc cacaccgtat
     7021 tcgttggcga ggcgtgaggt tacgacttca aactgcaaca cgccgaccgc acccaaaatc
     7081 aaatccgcgc cgctcatcgg tttgaatact tgaaccgcac cttcttcgcc gagttgttgc
     7141 aaaccttttt gcagttgttt gattttcagc gggtttttga tgcggacgct gcggaacagt
     7201 tcgggtgcga agaatgggat gccggtaaac gccagttgtt cgccttcgga gaagctgtcg
     7261 ccgatttgga tgttgccgtg gttcgggatg ccgatgatgt cgccggcgta ggcttcttcc
     7321 gccagttcgc ggttgtgcga catgaaggtt actacgctgg aggcggcgat ttcgcggttg
     7381 atacgcaggt gtttcatctt catgccgcgc tcgaatttac cggagcagac gcgcaagaag
     7441 gcgatacggt cgcggtgttt cgggtccata ttggcttgga ttttaaagat aaatccggaa
     7501 aatttcggct cgtccggccc gaccatgcgc atggtcgcgt cgcgcggttt cggtgcgggt
     7561 gcccagtcaa tcaatgaatt gaggatttcc tgaatgccga agttgttaat cgcagagccg
     7621 aagaacactg gcgtgagttc gccggcgaga aattcgtcga gattaaattc gttggaagcc
     7681 gcctgcacca attcgatttc gtcgcgcaac tgctggattt ccaacggaaa gcgttgttcc
     7741 aattcgggat tgtttatgcc tttgatgatg tcgaactcgt gcggcaggcg ttcgccgccc
     7801 gcttcaaaga gatagatttc gtcgttcagg atgtggtaca cgcccttgaa gtttttgccc
     7861 ataccgatcg gccaggtaac gggcgcgcag cggatttgca ggatgtcttc cacttcgtcc
     7921 aagagttcca aagaatcgcg cacttcgcgg tcgtatttgt tcatgaaggt aacaatcggc
     7981 gtatcgcgca ggcggcagac gttcaagagt ttgatggttt gcgcttccac gccttttgcc
     8041 gcgtcgatga ccatcaaggc gctgtccact gcggttaaaa cgcggtaggt gtcttcggag
     8101 aagtcttggt gtcccggcgt gtccaagagg ttgacggtgt ggtctttgta gtcgaactgc
     8161 atcacgcttg atgccacgga aatgccgcgc tgcttctcga tgtccatcca gtcggaggtg
     8221 gcgaatttgc cggttttctt acctttcacc gtgcctgcgc tttgaatcgc gcccgaaaac
     8281 agcagcagtt tttcggtcag cgtggtttta cccgcatcgg ggtgggagat gatggcaaac
     8341 gtgcggcggc ggcgcacttg gtcgaggatt tcttgggaca tggttttctt tgcaaaaagg
     8401 ttcaggccgc ttttcagacg gcccggacag tgtttgagac ggcgaaattg tacaaaaaaa
     8461 tgcctgataa ttcaatgttg gaggcggtca gtgcgtgctg ccgtaaatct ctttttcgtc
     8521 tttcaggacg gcatcggcgg tttcccacgc accgccgttc cattttttgt aaaagcagct
     8581 ttcgcgcccg gtgtggcagg cgatgccgcc gttttgggcg atgagcatca caatggtatc
     8641 gccgtcgcag tcgaggcgca gttcgcggac tttttgcgtg tgtcccgact cttcgccctt
     8701 catccattgt ttttggcgcg aacggctgta atagtgggca aagccggttt cgacggtttt
     8761 ttgcagggct tcggcgttca tccacgccac cattaaaaca cgtttggttt cggcatcttg
     8821 ggcgatggcg caaaccaaac ctttttcgtc aaatttgacg gcttcaagca ggtttttatc
     8881 catatttcct ttcagacggc atagtcgggg cggtcagagg cgcacttcga tgccggcttc
     8941 gcgcatagtg cgtttggctt cgcggatggc gatttccccg aaatggaaaa tgccggcggc
     9001 aagtacggca tcggctttgc cttcggttat gccttcaatc aggtgccgga cattgccgac
     9061 cccgccggag gcgatgacgg ggatgtcgac ggcttcggca acggcgcggg tcagcggcag
     9121 gttgaaaccc tgtttcgtcc cgtccctgtc cataccggtg agcaggattt cgcccgcgcc
     9181 gcgtttttgc atttcgaccg cccattccac cgcatccaaa ccggtcggat tgcgcccgcc
     9241 gtgggtaaag atttcccagc gtgtgttttc ggggttgacg gctttggcat cgacggcggc
     9301 gacgatggct tgcgaaccga aaaatccggc ggcttcgtta attaaatcgg gacgggtaac
     9361 ggcggcggtg ttgatgctga ctttgtccgc gcctgcattg agcaggcggc ggatgtcggc
     9421 aacggtgcgt acgccgccgc cgacggtcag cgggatgaag acttgtccgg caacctcttc
     9481 gatgatgtgc aggatggtgt cgcggttgtc ggatgaggcg gtgatgtcga ggaaggtcaa
     9541 ttcgtccgcg ccttcgccgt tgtagcgttt ggcggcttcg acggggtcgc ccgcgtcgcg
     9601 caaaccgatg aagttcacgc ctttgacgac gcgcccgtct tttacgtcga gacaggggat
     9661 gatgcgtttt gccagtgcca taatcggatg cctttagtcg agggaatctg ccagttgctg
     9721 cgcttgggca aaatcgatgc tgccctcgta aatcgcgcgg ccggtaatcg cgcctgctac
     9781 gccatgtttt tcggcggcac acagggcgcg gatgtcgtcc aagccggtca gtccgccgga
     9841 ggcgatgacg ggaatgcgga cggattgggc gagtttgacc gtcgtgtcga tgttcacgcc
     9901 gctcatcata ccgtcgcgcc cgatgtcggt gtagatgatg ctgttgacac cgtcgtcttc
     9961 aaagcgtttt gccaaatcaa ttacatgatg cccggttacg gttgcccagc cgtcgatggc
    10021 ggccataccg tctttggcat ccagtccgac gataatcctg ccggggaagg ctttgcacgc
    10081 ttcgcgcacc aagtcggggt ttttgactgc cgccgtgccg ataatcacgt cgttcagtcc
    10141 caaatccaaa tattgtccga tggttttcaa atcgcgcatg ccgccgccga gctgcacggg
    10201 gatgtctttg gcgacggcgg cgaggatgtc tttgatggcg ggcaggtttt gcggaacgcc
    10261 ggcaaatgcg ccgttcaaat ctaccaggtg caggcggcgc gcgccttgtt cgaaccagtg
    10321 cagcgcggtg tcggcgggcg aatcggaaaa gacggtcgcc tcttccatca gcccttgttt
    10381 caagcggacg caacgtcctt ctttcaaatc gatggcgggt atcagcagca tagtttttct
    10441 ccttgtgcgg ggccgtgtcg ggcttaccag tttaaaaagt ttttcaacat cgtcagcccg
    10501 gcatcgtggc ttttttcggt gtgaaattgc gtggcgaata cgttgtcttt gccgacgatg
    10561 caggcaaacg gggacgggta gtcgctttcg cccaatatgg tttcgggatt ttcgggggcg
    10621 aaatagtagc tgtggacgaa ataaaaacgc gtgttttggg ggatgccttg aaaaagcggg
    10681 tggttttggg tttggcgcac ggtgttccag cccatatgcg ggactttcag acggcatccc
    10741 tgcgggtcgc ggaggtcgcg ggcaaagcgt ctgactttgc cgccgaacca gcccaagccg
    10801 tcggtgtttc cttcttcgct gtggtcgaat aaaagttgcg caccgacgca gattccgaaa
    10861 aacggtttgt tttttaaggc atctttgact gcctcgtcca agccgccgcg cgtcagtgcc
    10921 gccatacagt cgggcatcgc gccctgcccg ggaaaaatga ctttgtcggc gcgggacacg
    10981 cggtcggggt cgccgcttaa aaagattttg gtattttttc cggcaagctg ccccgccgtc
    11041 cggacggatt tcaatacgga atgcaggttg cccataccgt aatcgataat ggcggtttgc
    11101 atggcttcct cctctttttt gcaatatggc tgcgatttta acaaacaaat gtgccgggct
    11161 gataaaaatg ccgtctgaaa acgggaatct gtcttcagac ggcatagggt tcaaacccgg
    11221 aaagccgttt gtcagccttc catttgtttt gcctgaacgg cagtcagggc gatggtgaac
    11281 acgatgtctt ccaccagcgc gccgcgagac aggtcgttga ccggtttgcg caggccttgc
    11341 agcagcgggc cgacgctcaa gacgttggca ctgcgttgga cggctttata ggtgcagttg
    11401 ccggtgttca ggtcggggaa gaccaaaacg gttgcctgtc ctgccaccgg gctgcccggg
    11461 gctttggatt tgcctacgcc cggcacggtt gccgcgtcat attgcagcgg gccgtcgatg
    11521 gcgaggtcgg ggcgtttttc ccgggcaagt ttggtggctt cgatgacggt atcgacatcg
    11581 gggccgctgc cggagttgac ggtggagtag gaaatcatcg ccactttcgg gtcgatgccg
    11641 aaggcttttg cggaatcggc agactggatg gcgatgtcgg caagctgttg cgcggtcggg
    11701 ttcggattaa ccgcgcagtc gccgaacacc agaacctgat ttggtagcag cataaagaat
    11761 acgctggata cgaggcttgc gcccggtgcg gttttaatca gttgcaaagc ggggcggatg
    11821 gtgttggcgg tggtgtgaac cgcaccggat accaaaccgt ccacatcatt ttgcgccatc
    11881 atcatcgtac cgagtaccac ggtgtcttgc agttgcttgc gcgcgtcttc gggtgtcagg
    11941 cctttggatt tgcgcagttc gcacatcggc tcgacgtatt gttcgaccaa tgaggcggga
    12001 tcgatgattt ccaaagagtc gggcaggctg atgccgcgtt ctttggcaac ggcttcgact
    12061 tcttcgcgtt tggcaagcag gacgcagcgg gcaatgcctt tttcgtggca gatggcggcg
    12121 gcttggacgg tgcggggttc tgcgccttca ggcaggacga tgcgtttgcc ggcttggcgg
    12181 gcgaagtcga tcaggttgta gcggaattgc gccggcgaca ggcgttttgc ttcgcggcct
    12241 gccaatacgg atacgtcttt cagcgcgtcg ctcgaaccga agaaggtcag gccggttttc
    12301 tcggctgccg cttcggcaac ggaggctgcc gcaccgtcca cgacaaaacc ttccaatacg
    12361 cccggcgcgg cggcgaagaa ctgtttggca aggttcaatt ggtgtgccag cgcgtcggca
    12421 tcggtattgt cggaacggac ggcgaagacg gctgccgcgt caagggacaa tgccagttcg
    12481 acgtttttgc ctgcgagata gattttgtcg gcatcgggcg cgatgccttc gatgacgagg
    12541 ttggcggcat cgagtgcggc aactttgccg accagtgcgt cgaaccagtc gtcgcttttg
    12601 ccttgcgtga gcagggtttc ggcggttgcg tcaacggctt ggaaaacttg tgcgtccagt
    12661 gcttttgcaa aggcttgtgc ggcggcggag gtgttcagtc cggcagatac gggtacgacg
    12721 agtacttttg ccataatata tcctttcgta tgctgcggtg tgcggcatat gtggttggaa
    12781 ggggcggcat atgggcagaa acggctgcct gcgtgccgtg cgtgccgtgt ttggcttggg
    12841 gcgcgcaggt tgaatatagc aaacaaattc tgtttccaac aagataaata tccgcaggct
    12901 tgtggatgct gccgcctttc agagggtatt tccggggaag aacagggcgg gaccgtccaa
    12961 atggaggacg gcggaaatgc cgtctgacag ggtgggggcg gaagggaggt tgagcgtgag
    13021 gacggtttgt ccggccctga ggctgatttc ggtatgccgc gctttgggcg tggttttgag
    13081 aaccacggcg tgaatggagg cggcgggtgc ggaatggggg tgaaggctga actgttccgg
    13141 acggatgagc agtgtaccgc gcgtgcctgc gggtgcgccg ctttggacgg gcaggcggcc
    13201 caatctgcaa tcggcggtgc cgtcggcgtt gagcgcggcg gggaacacga tgccttcgcc
    13261 gataaacagg gcggcatcaa ggtcggcagg ttgtcggtac aattcgtgag ggcttgcggt
    13321 ttggaggatg cgcccctgtt tcatcacggc aatccggtcg gcgtattgca gggcttcttc
    13381 gcggtcgtgg ctgacaaaaa cggcggattt tccgttggcg cgcagggcgg caatcatgtc
    13441 ttcgcgaatc tggcggcgca actgttcgtc cagcgcgctg aagggttcgt ccaacaaaat
    13501 cagttcgggg tcgggggcga gggcgcgggc gagggcgacg cgctgttgtt gtccgcccga
    13561 aagttcgtgc ggatagcgtc cggcaagttc ggaaatgccg gtcaattcca acatggcttc
    13621 gatgcgctgt cgctcttgcg ccgtcctgcc tttgccgttg ccgagaccgt aggcgatatt
    13681 gcggtaaacg gtcaggtggg ggaacagcac gccttcctgt acgaggtaac ccaaacggcg
    13741 ttcgcggacg ggaaggttgg tatttttcga gaagatggtt ttgccggaaa gcgaaatttc
    13801 gccggaatcg ggttgttcga aaccggcaag gcagcgtaaa agggtggttt tgccgcagcc
    13861 ggacgcgccg atgataaaga gaatttcgcc cgggtcgagg ctgagcgaaa tgtcgtttaa
    13921 aaccggggtg ttttgaaaac ttttggacag gtgtccgatg tgcagggcgg cggtcatagc
    13981 ggtgcttcct caagctgtta tttgaaggcg tatttcttca gcaggaatac gggtatgccg
    14041 gaaaataata ccagcatcag cgcgtaaggg gcggcggcgg cgtattgtgc gtccgatgtg
    14101 tattcccaaa cggcggtgga gagcgtgtgg acatcgtcgg cggtcagcag cagggtggcg
    14161 gtcaactctt tcatcagctt gaggaagacg agtgcgaatg cggcggtaat gccgggcagg
    14221 atggacggca gtaccaacgt cctgaaaata aagaagtgtc cgcgccccaa tgttgcgccg
    14281 acctgttcca tccctttggg gagttgttcc aaggaagtcc tcagggtggt ttgcgccatc
    14341 ggcaggtaaa gcatgaaata ggcaaggatg acgacgataa aggtttggta aacggcaggg
    14401 gtgtagttga tgctgaaata aaccaaggat agggcgataa ccaaaccggg gacggcgtgc
    14461 agtaaaaacg gcagcctgtc tatccaaacg gttaaaaaat tgcgatagcg aaccgatgcc
    14521 caaacaaggg gcaaggcaca taatatagtc aaaatcgcac ctaaagccga tacgcttaag
    14581 gaacggataa aggcatcaaa tacggatacg agcgcgaatg tgccggaagt gccgaccatc
    14641 atccaatgta tcaatacgcc aaaggggata ataatgccca aaatcaacaa gctgcttaaa
    14701 aaaacaatcg cgccgatctg accgggcagt ttgagggttt tgacgggata aggacgggca
    14761 acgcctttgc cgctgtggta aatcttggct ttgccgcgaa atatgctttc tccaaatacg
    14821 acgatgccgc acaccgccgt caaaacagcg gaaagcaggg cggcggtatt gttgttgtag
    14881 gacatttcgt attcttggaa aatggcagtg gtaaaagtgg ggtagttcaa aatggatacc
    14941 gcgccaaatt cgaccagcat atgcagggcg atcagtaaca cgctgctgcc gatggcgggt
    15001 ttgagctgcg ggaggatggc ggaaaaaaag gtttgcaggc ggcttttgcc caaggacagg
    15061 ctgacttctt cgtaagacag gctgatgcgt ttgagtgccg cctcgacggg caggtaggcg
    15121 agcggaaacg aggacaggct cataatcatc actgtccccc aaaagccttc gacgcggaag
    15181 gtcaggctga tccaggtgaa acagctgaca aatgcgggga tgcacaaagg cagggtgatt
    15241 gccgtctgaa aaaaggtttt gccgaagaag cggtaacgtt ggaacaaaag ggcgcaggca
    15301 atgcccaaaa caatggaaat cagggtaacg cccgccatca tcgtcaaggt gttggagagc
    15361 aaatcccaca tacgcgggcg gaacaacagt tcgacggcgc ggttgatgcc gacctgccac
    15421 gaacgcatag cgacatataa aaaaggcagg gtaagcggca gggcgatcag taggatgagg
    15481 ccggtaagcc aaatgggtat ttttttagga gacatagtgt ttttcatcgg caaaacgggc
    15541 ggacagtata aatgtccgcc cgtttgacaa tccgaaaacg gcttatttca taccggcttg
    15601 ctcaagcagc cgggtggcgt gttctttttc ggaaacagtg gtggcggaca cttggggtgc
    15661 ttccaacttg gcgatgggtt ccaaattgaa ggtggatacc acgtgcggat tcaaaggata
    15721 ttcggcacgg acggcggtca gggcgcgctg tccttccttg ccggcgagga aggcgacgaa
    15781 ttttttcgcc tcatccttgt tttgggagga ttttaacacg gctgcgccgg aataggtaac
    15841 gagtgcgccg ggatctctgt ggcggacgaa attcaggcgg gtgtggacat tttgtacgcc
    15901 tttttcacgc gcgaaagcgt gccagtagta gttgttgatg agggcggcat cgatttcgcc
    15961 gttttcaacc gcttgaaggg cgacggagtt tttagcgtaa ggcttgccgt attctttcag
    16021 acctttgagc catttcaatg cggccgcttc gcctttcagt ttgacgatgg cgacaatctg
    16081 ttccaagaac gcgccggaag tggggacgta accgatgcgg tttttccatt tcggcgtggc
    16141 gtaattcagg acggattttt ccaaatcttt ttcagacagt ttgcgggtgt cgtaaacgac
    16201 gacgcgcgaa cgtccgctca gtgccaccca gtcttttttg gcggcaaccg gcacgccttt
    16261 gccgcgtgtt tcgttgatgg tggaggcggg caggggctct aggaggttgg ctgcggaaag
    16321 ggtggcgagt gccgggattt gttcggaata gaatacgtcg gcggggcttc ggctgccttc
    16381 ttctttgatt tggccggcaa gctggtcgcc tttggcactg ttgagtttga ctttgatgcc
    16441 ggtagcccgg gtaaaggcat ctgcaacggc ttgtgccgct tctttgtgtt ggccgttgta
    16501 cacggtaatg tctgccagcg cgggggtggc ggcggtcagg gctgcggcaa gcagtgcgta
    16561 tcggatagat gttttcatat cgattttctc ctaaatgaat gagggtgtat accttgttaa
    16621 gacataacgg ggtgtagtgt attccttctt ttttataaat gcaaataatt attttttaaa
    16681 tttgttatta tccgatccgg ttattgtttg ttctgacttg tattttttcc gtgcatcgcg
    16741 cccgtaaggc ggaagcggcg ggcaatgtgt ggcggaatgt gggtaaaggc ggcattttga
    16801 tttgtcggaa tgctggagaa cctctccctt taaaacgccg tctgaacaag gttgccggaa
    16861 tagtattgcc atcccggcag atacagtttg tcgggatctt gccaatattg ttcatccaga
    16921 ctgttcggca gcgaggcggt tttgttgtcg agatgttttg ttaatccact atatatccca
    16981 catttctttt aggtttttac cttccgattg gaggtggcgg atttcttttc ccaatgcgtc
    17041 tgccgcccga tggatcagca ttgcgctgtg taccgcgccg atttttttca gaacggaaca
    17101 agtcttttcg gcggcgggaa aaccccagtt gcagacaaat tgcagtatgc cgccgttgcc
    17161 gatgtcggct tccgcccgcc aaatcaaaac cagctcctgt tcccgtccgt ccatgctttc
    17221 gagtttgccg tcatgctgtt caaaaagttt gtccaccgcc tgccgcatca tctgctcgaa
    17281 gcggtcggct tgggtatccg tatcggtcat agtattcttg ccttttaaaa atgccgtctg
    17341 aacatttctt cagacggcat ttgggggtta agctgacatt tcccgccagc gtttgacttg
    17401 gaggcgtacc tgttcgggcg cggtgccgcc caagtggttg cgcgcgttga ggctgccttc
    17461 gggtgtcaga acgccgtaca cgtcgtctga aatcagcttg ctgaagcctt gcagggcttc
    17521 gagcggcaat tcgctcaaat caacgccggc ttcatcggcg tggcgtacgg cttgggcgac
    17581 gacttcgtgg gcatcgcgga agggcatacc ttttttgacc agataatccg ccaagtcggt
    17641 ggcggtggca aaaccctgca tcacggcggc gcgcatattg ccgggtttga cggttacgcc
    17701 gcgcatcata tcggcgtaaa tccgcagcgt gtcgatgagc gtgtcggcgg tgtcaaacag
    17761 cggttctttg tcttcctgat tgtctttgtt gtatgccaag ggctgcgatt tcatcagggt
    17821 aatcaggccg ataaggtgtc cgatgactcg gccggattta ccgcgcacga gttcgggtac
    17881 gtcggggttt ttcttttgcg gcatgatgga cgaacccgta cagaagcggt cggcgatgtc
    17941 gataaagccg aaacgcggac tcatccacaa aatcaattct tcagacaggc ggctcaagtg
    18001 aaccataacc agcgaggcgg cggcggtgaa ctcaatggca aaatcgcggt cggatacggc
    18061 atcgagcgag ttctggcaga tttgttcgaa acccaacaat tcggcggtga tttcgcgctg
    18121 aatcggatag gtcgtaccgg caagggcggc agcgccgagc ggcatacggt tgacgcgttt
    18181 gcgacagtcg gccatgcgtt cgaaatcgcg tccgagcatt tcgacgtagg cgagcatgtg
    18241 gtgtccgaag ctgaccggct gggcgacttg cagatgggta aaaccgggca tgacggtttc
    18301 ggcgttttgt tccgccaaat ccaataatgc cgtctgaagg ttttgaatca ggttttggat
    18361 aacggtaatc tggtcgcgca gccaaaggcg gatgtcggtg gcgacttggt cgttgcggct
    18421 gcggccggtg tgcaggcgtt tgcccgcgtc gccgattttg tcggtcaggc ggagttcgat
    18481 gttcatgtgg acatcttcca aatcgagcgg ccattcgatt ttgccgctgc ggatttcttc
    18541 gaggatttcc gacatgcccc gacggatgtc ggtcagatcg ttttcactca gcacacccga
    18601 ccgggtcagc atctgcgcgt gggcgagcga gccttggatg tcccattcgg caaggcgttg
    18661 gtcgaaaccg atggaggcgg tgtattgttt gacgagttcg gaaacgggtt cgttgaaacg
    18721 tccggaccag gttttgtcgt gcataaggat tccttgatgg ggttattcgg tgcggtattt
    18781 ttccaaaagg cggcggaagg gttcgccggt ttcggggtgt tttaaaccgt aggcgacggt
    18841 ggcttcgaga tagccgagtt tgctgccgca gtcgtagcgc gtgccttcaa aggggtgcgc
    18901 caagacaaat tcgtgatcga gcagcttggc gatgccgtct gtaagctgga tttcgttgcc
    18961 cgcgccgcgc ggcaggccgg ttaaaaggtc gaaaatgcgc ggggtgagga tgtagcgtcc
    19021 gacaacggca aggttggagg gcgcgtcttc gggcttgggt ttttcgacaa tgccggtaat
    19081 gcgttggaac tgtttgagct gttcggtttc gacgatgccg tatgagccgg tttgcgacgg
    19141 ttcgacggtt tctacgccca aaatgctgtt accgctgcgc ccgtacactt cgaccatttg
    19201 tttgagcgcg cctttgggcg catcgattaa gtcgtcggca aggatgacgg cgaagggttc
    19261 gtcgccgatg gcggcgcggg cgcacaagac ggagtgtccc aatcccagtg cttccgcctg
    19321 acggatgtag aggcaggtaa tgttcggcgg caggatgttg cggacgtgtt ccaacaattt
    19381 gtctttatgg cgcatttcca acttagtttc gagttcgtat gccttgtcga aatggtcttc
    19441 gatgctgcgc ttgttgcgcc cggtaacaaa caccatttcc gtgcagccgg cttccacggc
    19501 ttcttctacg gcgtattgga tcagcggctt gtcgacgatg ggcagcattt ctttcgggtt
    19561 ggccttggtg gcgggcagga agcgggttcc catccctgca acggggaaga cggctttttt
    19621 tatcggtttc attctttttc ctttgtattg ttttgatgtt taaagggcga gtttgcgaat
    19681 taaatcggcg agtgcctgcg cgcggtggct ttcgcggttt ttgacctccg aatccaattc
    19741 ggcggcggtt ttgccgtgtt cgggcagata aaaatacggg tcgtaaccga aaccgttttg
    19801 cccgagcggc gtgtggtgcc attgtccgtg ccatatgccc tcggcgataa tcgggcgcgg
    19861 gtcgtcttta tgacggacaa aaaccaatac gcagacataa gaacaacttt tgtcggcctt
    19921 gccggcaagt tcggcggcaa gtttcaggtt gttggcggta tcggatttgg gattgctgcc
    19981 ggcgtaacgt gcggaatgga tgcccggcgc gccgtttagg gcggcggcac agatgccgct
    20041 gtcgtcggcg agtgcgggca gcccgctgtg tccggcggcg tgccgggctt ttgccagcgc
    20101 gttttcgaca aaggtgggat agggttcggg gcattcgggt atgccgaatg cggattgcgg
    20161 caatacggtg atgccgtaag gtttgaataa gttgccgaat tctttgagct tgccggcatt
    20221 gccgcttgcc aaaacgattt tttccggttt ttcagacata gcggtttccc tgtgatgaag
    20281 atggggcggc gcgtagggat ttgtgccgca ggtagagggc gagtgcgccg atttgcccga
    20341 acagcgcgcc gaatgcgaaa aggaaggacg cgacggcgaa ggctttgctg ccggcggtca
    20401 gcagataagc acccaagagg acgggcagca taaatgccag tgtgaacaag gcaacagaca
    20461 acagatagat ttttcggcgg ttcatggcgt tcggtcggaa acgggatgtt cggattatag
    20521 ccgattagga cggcattccc tagaggctgg aaaaatgccg tctgaagcgg cgttcagacg
    20581 gcatggcggg cattatgcct gtttgttcca acgttcgatg gattctttga tgactttttt
    20641 cgcttcttcc gcaccgcccc aggattcgac tttggtcgta cccgcttttt tcaggtcttt
    20701 gtagtggttg aagtggaact cgatttgttt gatgagctgt tgcggcaaat cggacaaagt
    20761 tttgtaggcg ttgccgttat tgcggtcgtc ggcaggtacg cagacgattt tgtcgtccac
    20821 ttcgccgtcg tcgacgaatt tcatcacgcc gataacgcgc gcttccaaga atacgccggt
    20881 tgccagaggt tgttcggtaa cgagcaacac gtccaattca tcgccgtctt cgtccaaagt
    20941 ttgggggatg aagccgtagt tggtcggttt ggcgaagatg gcgggttcga tgcggtcgag
    21001 ttggaatgcg gcgagtttgc ggttccactc gattttgtgg ttgctgccgg cggggatttc
    21061 gttgacgacg ttgatgatgc cgccgtccac gtcgcccggg gtcaggattt ggttgaagtc
    21121 tgccatttgg tttcctttat ttgggaaggt ttgaagtttg aaagtatagc acaaacgtcc
    21181 ggctggaaat gcgtccgatg ccgtctgaaa gggaaaccgg gcgcgtgtta ccgtttgccc
    21241 aaaaacctgc ccagttccaa aatcgcgcgc ctgttggacg gggaaaacac tttttccgcc
    21301 gcttcttcca agccgaacca gccgtaggag acgtgttcct cgggttgcag gacgacgggc
    21361 gtatcgcgcg ggatttcggc ccggaagacg tgttcgcggt tttcaaacac gcctttgggg
    21421 tagcggtgcc gccagtggtg gtagatttcg taaaccgtgc tgtcgtgccg gtcttggagc
    21481 tgcccgtcct ccagcaggat gccggtttct tcccaaactt cgcgccttgc cgtttgggcg
    21541 acggtttcgc ccggttcgag gctgccggtt accgactgcc aaaatccttt cggatgcgtg
    21601 cgttcgatga gcaggatgcc gccgtccccg ctataaagga cgaccagtgc ggagacgggg
    21661 tatttgagcg gttttgccat cggcatcttt cggcgggctg cggtaatgaa ggggcggatt
    21721 atagcaaacg ccgcacgtta tggcgtttat ccttttccgt atcctttttt ctgcacggat
    21781 gggacgcgcc ggtgtttgcc ggtaaatttt cctttgtgtc aaaaagataa gggcggttgt
    21841 gattttaatg cttgccaaag tgacgggcgg aaactataat ccgcaactta ccgagtcgga
    21901 gtgtggcgca gtctggtagc gcacttgcat ggggtgcaag gggtcgaagg ttcgaatcct
    21961 ttcactccga ccaaaaattc cgaaagccgc tttcaaaagc ggcttttttg ccgtccgtat
    22021 gattatgatg tagagtacgc ggcgacagac attcaaatgc cgtccgaaaa ccgttcagac
    22081 ggcatctctt tatctt
//
LOCUS       DAXFHF010000034        21618 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXFHF010000034 DAXFHF010000000
VERSION     DAXFHF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.034"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            119..505
                     /locus_tag="ACFIRM_001179"
     CDS             119..505
                     /locus_tag="ACFIRM_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028817.1"
                     /translation="MWKIYKENSTDLNFALGSIYCQAINLTEFKMWVEKIIREMDLDE
                     IPNYFFDLTDFQSLFHLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALKKHPEIYQRFSYFFPFVELPPL"
     gene            653..1042
                     /locus_tag="ACFIRM_001180"
     CDS             653..1042
                     /locus_tag="ACFIRM_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028818.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINFSEFKLWLDIVVRDTPIDT
                     VPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNNQKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYHKFKRFFPFVELPLL"
     gene            complement(1082..2539)
                     /locus_tag="ACFIRM_001181"
     CDS             complement(1082..2539)
                     /locus_tag="ACFIRM_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC3028819.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILASALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIC
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(2554..3384)
                     /locus_tag="ACFIRM_001182"
     CDS             complement(2554..3384)
                     /locus_tag="ACFIRM_001182"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC3028820.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(3461..3850)
                     /locus_tag="ACFIRM_001183"
     CDS             complement(3461..3850)
                     /locus_tag="ACFIRM_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3028821.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(3997..4524)
                     /gene="nspA"
                     /locus_tag="ACFIRM_001184"
     CDS             complement(3997..4524)
                     /gene="nspA"
                     /locus_tag="ACFIRM_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC3028822.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYVGKVNTVK
                     NVRSGELSAGVRVKF"
     gene            complement(4967..6142)
                     /gene="hemW"
                     /locus_tag="ACFIRM_001185"
     CDS             complement(4967..6142)
                     /gene="hemW"
                     /locus_tag="ACFIRM_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC3028823.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTVEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGTGLIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(6199..8670)
                     /gene="ligA"
                     /locus_tag="ACFIRM_001186"
     CDS             complement(6199..8670)
                     /gene="ligA"
                     /locus_tag="ACFIRM_001186"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC3028824.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAALNQRQTENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGTVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(8804..10090)
                     /locus_tag="ACFIRM_001187"
     CDS             complement(8804..10090)
                     /locus_tag="ACFIRM_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC3028825.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQALHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(10284..10856)
                     /gene="ampD"
                     /locus_tag="ACFIRM_001188"
     CDS             complement(10284..10856)
                     /gene="ampD"
                     /locus_tag="ACFIRM_001188"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC3028826.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            10940..11935
                     /gene="mltG"
                     /locus_tag="ACFIRM_001189"
     CDS             10940..11935
                     /gene="mltG"
                     /locus_tag="ACFIRM_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC3028827.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            11995..12615
                     /gene="tmk"
                     /locus_tag="ACFIRM_001190"
     CDS             11995..12615
                     /gene="tmk"
                     /locus_tag="ACFIRM_001190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC3028828.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            12837..14117
                     /locus_tag="ACFIRM_001191"
     CDS             12837..14117
                     /locus_tag="ACFIRM_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC3028829.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCVEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILSPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            14519..15550
                     /gene="lpxK"
                     /locus_tag="ACFIRM_001192"
     CDS             14519..15550
                     /gene="lpxK"
                     /locus_tag="ACFIRM_001192"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC3028830.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            15756..16289
                     /locus_tag="ACFIRM_001193"
     CDS             15756..16289
                     /locus_tag="ACFIRM_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC3028831.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            16274..16381
                     /locus_tag="ACFIRM_001194"
                     /pseudo
     CDS             16274..16381
                     /locus_tag="ACFIRM_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            16511..16693
                     /locus_tag="ACFIRM_001195"
     CDS             16511..16693
                     /locus_tag="ACFIRM_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC3028832.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            16690..17451
                     /gene="kdsB"
                     /locus_tag="ACFIRM_001196"
     CDS             16690..17451
                     /gene="kdsB"
                     /locus_tag="ACFIRM_001196"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC3028833.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            17474..17989
                     /locus_tag="ACFIRM_001197"
                     /pseudo
     CDS             17474..17989
                     /locus_tag="ACFIRM_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            18146..18238
                     /locus_tag="ACFIRM_001198"
     tRNA            18146..18238
                     /locus_tag="ACFIRM_001198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18180..18182,aa:Ser,seq:gct)
     gene            18511..19296
                     /gene="trpA"
                     /locus_tag="ACFIRM_001199"
     CDS             18511..19296
                     /gene="trpA"
                     /locus_tag="ACFIRM_001199"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC3028834.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            19333..20205
                     /gene="accD"
                     /locus_tag="ACFIRM_001200"
     CDS             19333..20205
                     /gene="accD"
                     /locus_tag="ACFIRM_001200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC3028835.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(20265..20876)
                     /locus_tag="ACFIRM_001201"
     CDS             complement(20265..20876)
                     /locus_tag="ACFIRM_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC3028836.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            20984..21208
                     /locus_tag="ACFIRM_001202"
     CDS             20984..21208
                     /locus_tag="ACFIRM_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC3028837.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            complement(21242..>21618)
                     /locus_tag="ACFIRM_001203"
     CDS             complement(21242..>21618)
                     /locus_tag="ACFIRM_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028838.1"
                     /translation="GIFRRLSDKSVKMFQKHGTVCPLGFHTATVARQAQIINAAIAFK
                     RPQRLYIGFCNSGRQTGQVFLSDRDKMSDSGKLGNRFETTLSQDAGTPKQIDGYACKI
                     LMPYTGLPVLGITRSKHKCRQQ"
BASE COUNT         4766 a         5238 c         6506 g         5108 t
ORIGIN      
        1 gggtaaccaa agaaatgttt gacggcaaag gaacatctaa aataccggat tacacttggc
       61 atcatcatca agatacagga tgtatcggtt ggaaagcgat gaacaaaaga agataattat
      121 gtggaaaatt tataaagaaa atagcactga cttaaatttt gccctaggca gtatatattg
      181 ccaagcaatc aatcttaccg aatttaaaat gtgggtcgaa aaaatcataa gggaaatgga
      241 cttagatgaa attccaaatt atttttttga tctaacagat tttcaatcac tattccatct
      301 aattgatatt ataggatttg ttcccgaaaa taacttatca aaaaatcagg ataatgcatt
      361 aacaggcatt gccttcttaa gggggataga tgtctatgac cctcccgttt caaaagaaaa
      421 agcattaaaa gccttaaaga aacatcctga aatttatcag aggttttcgt atttctttcc
      481 gtttgtagag ctgcctccgc tttaaacagt caaaatgccg tctgaaacga tattcggctt
      541 tcagacggca tttttgatat aaagcgggta accaaagaaa tgtttgacgg aaaaggagaa
      601 gctaaaatac cggatgtatc ggttgggaag caatggataa aggtaaataa ttatgtggaa
      661 aattagtaaa gaaaattgtg aagatttagg atttgcaata gtctgtatgt tctatgatgc
      721 tattaatttt tctgaattta aattatggtt ggatatagtt gtcagagata ctcctattga
      781 tacagttcca ttgtatattt ttgatttgat tgattttgat aagagtatag gggaaattta
      841 tgatgtaatt ggagtcgtta attatggtta catttcaaat aatcaaaaaa atgcattaac
      901 gggcattgcc ttcttaaggg ggatagatgt ctatgaccct cccgtttcaa aagaaaaagc
      961 attaaaagcc ttaaaaaaac atcctgaaat ttatcataaa tttaaacggt tctttccttt
     1021 tgtagagctt ccgcttcttt aaaggacaat atgccgtctg aaaagttttc agacggcatt
     1081 tttatttctt ccagtaggcg ggggtgaaga ggatgaagac ggtgaagatt tccagcctgc
     1141 ccaagagcat tgcggtaacg cagatccatt tctgcatcac gtccaaaccg gcgtaattgc
     1201 cggcgggccc gacttcgccc agtccggggc cggcgttggt aatgcaggcg atgacggcgg
     1261 taaaggcggt ggtaaattcc ataccgctcg ccatcagcag gaagctgaag aggacgacgg
     1321 tcataaagta gatgaagatg aaggacataa cggtcagcgc gaggcggtcg ggtatggctt
     1381 tgccgctgat tttgacggtg cggacggctt tggggtgcag cagcaccatc atttcgcgca
     1441 ggctgaattt gaacaggacg agggcgcgta tggttttgat gccgccgccg gtcgagccgg
     1501 agctggcaag gatgttggcg aggaaaaaca tccacaggca aatcaggagc ggccattgtg
     1561 cgaagtcggt gttggacagc ccgtttgcca atccgatgga gacgaagttg aaggcggtgt
     1621 agcgcaggga ttcgccgaag ccggcgtaat ggccggtgtg ccacaggtac agggcggagg
     1681 caaggatgct gccggagagc agcagcagca tcgtccggca ttcttcgtct ttccaatagg
     1741 ttttgaggct gcggctgttg agggcggcga aatggctggc aaaattgatg ccgccgaaaa
     1801 tagtgaaaac gatgatgact gcttcgatga ggggggagtt gtaataaccg atgctggcat
     1861 cgtgggtgga aaacccgccc agcgagaggg ttgccagcgc gtgacagacg gcatcgaacc
     1921 aacccatccc ggcaaaatgc aggcaggctg ccgcgaggat ggtgatcagg gtgtagccga
     1981 accagagttt tttcgccact tgggaaatgc gcggcgacat tttgctttct ttgtcaatgc
     2041 cggggatttc ggctttgaat aactgcgtgc cgcctacgcc gagcataggc aggatggcga
     2101 cggcaaggac gatgatgccc attccgccca gccagttgag catatgccgc caaaagttga
     2161 cggagggggc gagcccgtcg acgtggggga tgacggtcgc gccggtggtg gtcagtcccg
     2221 acatcgattc aaaaaatgcg tcggtaaagc ccatattcgg gaaatacagg tacatcggca
     2281 tcgcagccat agcggcaaac gccagccaca acatcaggac gagggtgaag ccgtcgcgcg
     2341 ggcgcagttc gcgcctgaac cggagggtgg cgagccggac gatgcacgag ccggaaaggg
     2401 taacggtcgc ggtggtggcg aaggcggtgt acgcaccgtc cgaaaaggcg taggagaggg
     2461 cggcgggtat cagcaggata aaggaaaaca gcatacccag tcgggagagg acgtgggcga
     2521 tgggcaggat tttgtgcata gtggggcggt ccgttatttt gcgaagcttt tccagtctat
     2581 gccgccggcg gcttggactt gggtaatttc ttccgtttcg aggttgacgg cggtcagctg
     2641 cccgccccac agcgcgccgg tgtccagcga gatgacgttg tcggcattcg tgtagcccag
     2701 cgaggaccag tgtccgaaga tgatggtgtg gtcgaggttt tgccgatcgg gggctttgaa
     2761 ccacgggcgc aggtaaggcg gcattttttt cactgtggat ttgtagtcga aatccagttc
     2821 gtttttaaag gtcagggcgc gcatccgcgt gaaggcgttg acgatgaagc gcaggcgggc
     2881 atagccttcc aaaccttcgt cccatgcggc cggtttgttg ccgtacattt tggagaagaa
     2941 tttgacgtat tttttgccgc gcagttcggc ttcggcttct ccggcgagcg attcggcttt
     3001 ggctatgcgc cattgcggca ggatgccggc gtgtatcatc acgcggccgc cctcgcgtat
     3061 caaaagcggt tgcgcgcgca gccagtcgag catttttccg ccgtcggggt gtttgagtat
     3121 gggttcgatt gtgtcgctgc gtttgagcgc gccttcgccg cagccgacgg cgagcaggta
     3181 caggtcgtgg ttgccgagga cgatttgcac gctgttttcg tgccggatgc agaattgcag
     3241 cgtttcgagg gatttcgggc cgcggttgac gatgtcgccc gtcagccaga gggtgtccgt
     3301 gccgtggttg aagccgattt tgccgagcag cgcggtcagt tcgtcgaaac agccttggat
     3361 gtcgccgatt gcgtaatgtg ccattgcggg tgttgtgaag tgggaaagtg ttgcggttcg
     3421 gacggcacgg ttggaaatct tatgccgtcc gaacgtggaa ttatgcgttc aaaacgagga
     3481 cggcttccgc ttcgacctgc acgcctttgg gcagcgaggc aacgccgacg gcggcgcggg
     3541 cggggaacgg ctcggtgata aattccgcca tcacttcgtt gaagacggca aaattgccca
     3601 agtcggtcag gtaggcgttg agtttgacga tgtcgtccag cgaaccgcct gccgcttcgg
     3661 cgacggcctg caggttttgg aacacttggc gcgcttcggt gtggaaatcg ccgttgccga
     3721 ccacagtcat cgtggcggga tcgagcggaa tctgaccgct catgtaaacg gtgccgccgg
     3781 cgcgcacggc ctggctgtat gcgccgatgg cggcgggggc tttgtcggta tggatgacgg
     3841 ttttggacat ttcggattcc tcaaaaaata gggcggcaga agccgcagcg ttcgggatta
     3901 tcgtacaaaa ccgccggctt gtgtagtttt aggcggtata tgtgccggaa acaaaaaacc
     3961 gccgaaggct cggcggtttg cggaataacg cgtatatcag aatttgacgc gcacgccggc
     4021 ggacagttcg ccggaacgga cgtttttgac agtgttgact ttgccgacgt agttgtagcg
     4081 gtagccggca tccagatcga cattgggtgt aacggcatag cttacgcccg ccaatacgcc
     4141 gaggccggcg gaggttttgc tgaagctgtc gctgccgccc aagtgggcgg aagcgcggtt
     4201 gaggctcaaa cgcgcgccga aatacggttt gacgggcgat tgggtgtcga agtcgtaaat
     4261 gacggacgcg ccgatgctgt aaagtttgaa atcggtggat ggggcttgtt tatagttttt
     4321 gtagcgcgtg taatcgacgg cgaagcggag gtcgttgatg cggtagcctg cggagatgcg
     4381 cgggctgaag cctttggcag aacctaaaga gcttgaggct ttggcgtgtg cggcatcggc
     4441 ttggacgtaa aagccggatg cgccttccgc cagtgcggcg gccgggagtg cgagggcaat
     4501 cagtgcggca agtgcttttt tcatattttg gttcctttat ggtcagggag aaaaattgtt
     4561 aataatccgt taaagaatcc tgccgtatta tactgaaatt ttctttttgc atcgtaatat
     4621 tttcaatgcc tcaagatacg cggcggcatc cggctgcttt gccgacggca aagccgttaa
     4681 cccgcgcgtt gcccttaaac ggcggcatca cgcggcggat gggtgaaact tgcaaacggt
     4741 ttggaaaaaa ccgcaaaggc ggggcggcgg catttgtcag attgttgcag gcgcaggcgt
     4801 acggtttttt gtgtgcggcg ttaccttagg cgtcggacat ttccggcggc ggctgtgccg
     4861 tctgaaacgc ccggcggggg atgcggctgc gtttcccatc gataggcata tttgccggcc
     4921 gcgttcgggg cgggttttac ccgggcggcc gccgatttgt ttgcgcttat aaaaaacact
     4981 gcaacaaatc gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg gtcgggtcgg
     5041 tttccagcag gcctttttgc cttgccgttt cgatttgcac catgattttg gcggcgggta
     5101 cgcccgtgcg ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc agggcgttca
     5161 tcatgaattc gaacggcaaa tcttcggcgg cgacggtttt gcgttcgacg gcttcgcccg
     5221 gctgactttg cattaaggcg aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga
     5281 tgcggtcggg ataggagatt ttgccgtgcg cgcccgcgcc tatgcccaaa taatcgccga
     5341 actgccagta gttcaaattg tggcggcact gcatggtggg tttcgcaaaa gccgatgttt
     5401 cgtagtggat aagacccgtg ccttccagtg tgccgtgtac cgcgtcttcg atgtcgagcg
     5461 ccgcttcgtc ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg ttcggctcca
     5521 tcgtcagatg atatgcgctg atgtgggttg cgtctgtggc gatggcggtt tgcacgtcgt
     5581 ccaatgccgt ctgaacggtt tggttcggca gggagtacat caagtcgata ttgactttat
     5641 caaataattt taaggcggta tcgatagcgg ttaaggcttc cttgccgttg tggacgcgcc
     5701 ccagccttga gagcatatcg tcgttgaagc tttgtacacc aatagaaagc cgcgtgatgc
     5761 ctgcgtcttt aaatccttga aacttctcga tttcaactgt acccggattg gcttccaacg
     5821 taatttccgc ttcgggctgc aagcgcaaca gcgaacgcac gccgcttaac aaacggtcaa
     5881 tcgattccgc ctgaaacagg ctgggcgtac cgccgccgaa aaatatcgtt tccaccggcc
     5941 tgccccaaat attgggcaat tcaagctgca agtcggtcag cagcgcgtcg atataggcgg
     6001 cttcgggcaa tccgtttttc aggctgtggg aattgaagtc gcaatacggg cattttttga
     6061 tgcaccacgg gatgtggata tagagcgaca ggggcggcag ggcggtgagt cgggtgcggt
     6121 ttggaaagga aatggtgtgc atggtgcggt tcggaaaagt ggacgatgcc gtctgaaggc
     6181 ggttcggacg gcatgggttc agcagagcag ggtaagcagt tcggcttcgc tgaggacgga
     6241 aacgcccaag gcattggctt tttccagctt gctgcccgcg gcttctccgg cgacgacgta
     6301 gtcggttttt ttggacacgc tgccggacac tttgccgcct gcggcttcga ttagggcttg
     6361 ggcttggtcg cgtttgaagg tgggcagggt gccggttaac acaaaggttt tgcctgccac
     6421 ggctttattg atgccgtctg aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa
     6481 gaaggttttt aggttttcga gcagggcggt gttttgcgct ttgctgcgcc acgcctgcca
     6541 gtcggcaggg agggctttgt cgttttgcag tccttctata ctttgtccgg cgagttccca
     6601 taaggcttgg gctttgtttt cgctgatttt gaaaccgggc aggcgggtaa tccagcgttg
     6661 cggcccggca tattgtgcgg caggcaggct gacggcttgg gcttgcgggg caacgcctgc
     6721 ggcgagcagc tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga
     6781 acgcgccact accgtgccga tgtcgggcag gcaggcgagg acgggttcgg gggcgcggcg
     6841 gacgcgttcc aatgtgccga atgcctgcgc cagcgttttg gcggtgcgtt cgccgacgtg
     6901 gcggatgccg agcgcgaaca ggaagcgggc aagttcgggc gttttgccgg attctatgcc
     6961 tgcgaggatg ttttgcgccc acttggtcgg ttgttttttg ccgttttggg tattgtattt
     7021 agacaggtcg cctgaaaccg tttctgcgtc gccgttttcg ttttcagatg accctttatc
     7081 cgccgtttcc ttcatttttt gcagggtcgg aatatcgatg cggtagaggt cggcgaaatg
     7141 gcggacgagg tcttgggcga ccagctgttc gatttgtttt tcacccaagc cgtcgatgtc
     7201 catcgctttg cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat
     7261 accgccgctg catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat
     7321 ggggcagcgg gccggcaggc ggtagggctt atggagcgga acggattcgg tttgcttggc
     7381 ggacggtgtt tcggcaaaca ggtcgtcctg ctgatgcccg atgccgtctg aaacggcaac
     7441 ggcggtttcc tgcatcgggc ggcgttcgaa aatcacgcgc acgacttcgg gaatcacgtc
     7501 tccggcgcgg cgcacgacga cggtatcgcc gacgcgcacg tctttgcgcg atacttcgtc
     7561 ctgattgtgc agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgcaggcg
     7621 ggcaaccggc gttaccgcgc ccgtcctgcc gatttgcacg tcaatcgctt cgacaatggt
     7681 cagggcttct tcggcaggga atttgtgggc aaccgcccag cgcggcgcgc gggagatgaa
     7741 gccgagttcg cgctgttgcg ccaagctgtt gactttgacg accgtgccgt cgatttcgta
     7801 gggcagttcg gggcgttttt gctgcatgtg ttcgtaaaac gccaatactt cgccgatatt
     7861 tttgaaacag ccgaaattgc cattgggcag actgaagccg agttcttgga aataggcgag
     7921 ttcttggatg tgttcttcag cttcgaaacc gccttgctga cgggcaatcg aataggggaa
     7981 aaagtgcagt ttgcgttgcg cggtgatgcg cgaatcgagt tggcgcaggc tgccggcggc
     8041 ggcattgcgc ggattggcga aaggtttttg cccgttttcg gtctgacgtt ggtttaaagc
     8101 ggcaaaatcg gctttgagca tcagcacttc gccgcgtact tcgatgagtt cgggcacgtt
     8161 ttcgccgtgc agccgcaggg ggatgttgga tacggttttg acgtttcggg taacgtcttc
     8221 gccggtcgta ccgtcgccgc gtgtcgccgc ctgcaccaat acgccgtcgc ggtagagcag
     8281 gctgatggcg aggccgtcga atttgggttc gataacgtat tcgggattgc cgccgtccaa
     8341 gccgtcgcgt acgcgttggt cgaaggcgta catttcggca tggtcgaaca cgccgttttc
     8401 atcttgcggg gaaaaggcgt tggtcagcga cagcatcggc acttcgtggc gcacttcggc
     8461 aaagcctgcc aaaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gcttgagctc
     8521 gggatggttt cgttccaacg cttcgagttc gcggaacagg cggtcgtatt cggcatcggg
     8581 tatgctgggc gcgtcgaggg tgtagtattc gtaggcgtag cggttgagga ggtcggtgag
     8641 ttcgtgtatg cgttgtgcgg tcgggttcat ggattcgtac aggttcagac ggcatggaca
     8701 aatgccgtct gaacggtaaa acgtgttgga aatggtcgga ttttacctga aaacaaaagg
     8761 cggatgcacc gtcgccgatg tatccgcctt tgtgtttgcg gagttatgaa aacaggcgca
     8821 gggcggtttt gccgcccggt tcgataccga ctttgagcat ctcggactga cgcgccaata
     8881 cataagtgcg tacgtctttg agccattggg tcgaaacttc ttccattttg tcgttgacca
     8941 gattcaggtt caactgaccg gacaggcgta ccgccaaatc cataaacaaa tcgtcgaagg
     9001 ttttttcgcc tgccggagag tgcgggatgt cgagcagcat actgaagcct ttgtaggact
     9061 ggttgtccaa aagggcattg gtaaacggct cgttgttgag cgagcagatg gagaacatgg
     9121 tcgagcccga cgtgtcggta tagtggaacg cgccgtcgtc ttccaaaacg aaacccacgc
     9181 ccgttacggc ggaacgcagt tctacgccgc tgatgctggt cggcgaaacc aaatggatgg
     9241 cgatggtctg gtcgacgcgc gcgcagaatg cgtccagtgc ggaagccact tcgataaagg
     9301 cggcaaggtc ggtgtgcagc gcctgaccgc ccatgctttg tgcgaatgcg tccgcctggc
     9361 ggttgaatgc ggagagttct tcctgcgagg caagtccgtt gcggctgact gcctggatac
     9421 ccacgataaa tgcctgatag cggatgcccg ggatgggttc ggcaatctgg aaatggtcgt
     9481 ccatggtgca gccgacaatc tggtagcggc agcggttgga aaggcgcggc agtgcgtgca
     9541 gttctttggc ttcggtcagc gcgatatagg agatgaagtc gaagcgcacg tcaaaccagg
     9601 gcagttcgac cttcgacagc tctttgagcg taatcagcgg ttttgccggc gtttgcggaa
     9661 cgggaacggg ttttgccggc gcgtcggcag gtttcggtgc ggaatgtccg gtttggggtt
     9721 cggagacggt gtgggcggag ttgccgataa tgccgctttc ttccaaggcg gtttcgattt
     9781 cggttttgaa cggggaggct tttgcctgtt tctgcttggc gatgtagacg gcatcctgtt
     9841 cttgcaggtt gcgcatggcg gagtcttggg gtttggccgg ttttttgacc gccggttggg
     9901 gtttcggcat catgactggc ccgccggacg gtttgccgtc gcggacatgg ctggttttgc
     9961 tgttgagcag ggcatctttg tcggagtgtc cgaactggtc gcgcactttt ttgcggtatt
    10021 ggttttcctg atacatattg taggcgacaa cggcgaggac ggcggcgagg aacagtacga
    10081 tgtaaatcat ggcaatcact ttaaatttcg ggatgcagga tgcgcaaagt gcgggtactg
    10141 cggttaaatc gggcttgcgt ttccggcagt ctgacggaac ggccggttat aacgtttgaa
    10201 ttataacgaa aattgcaggg tctgacagca gtgtgtcgaa ataagcggaa attttccgaa
    10261 atgccgtctg aaatctgtgg ttttcagacg gcatttctgt ccacgggaaa ccctttttcc
    10321 cgtatccgcc gccagtcgaa aaaatggccg gggtcggttt tgcggccggg tgcgatgtct
    10381 tggtgtcccg ttaccgccgt gatggggtag cggcggcaga gggctgccaa cagcgtttcg
    10441 agcgaacggt attgcgcttc ggcaaacggt tcgaaatcgc agccttccag ttcgatgccg
    10501 atggaaaacg cgttgcactt ctcccgcccc cgaaacgagg atgcgccggc gtggtacgcc
    10561 atgtcgccgc atgatacgaa ctgcaccgtt tcgccgtcgc gtgcaatcag gaaatggctg
    10621 gacacgtgca aagtgtgtat caggctgaag aacggatgtc cgtcggggtc gagccggttg
    10681 gcaaacagct tttccaccgc atccgtgccg tattcgaacg gcggcagcga aatgttgtgc
    10741 agcacgatca gggaaaccgt ttctcccgtt tccctcgggc tgaaattggg cgacggggta
    10801 tggcgtatgc tttgaagcca gccgttttgc cagtgtgctc cggcgtgatt gtccatgatg
    10861 ttcttcctgt ccggcgggca atttgggtta tactgtcgcc cgaattttaa gacgtattcc
    10921 gaatgctggg aatcctacca tgttgaaaaa aatgttgaaa tggactgccg tttttttgac
    10981 cgtatcggca gccgttttcg ccgcgctgct ttttgtccct aaagacaacg gcagggcata
    11041 ccgaatcaag attgccaaaa atcagggtat ttcgtcggtc ggcaggaaac ttgccgaaga
    11101 ccgcatcgtg ttcagcaggc atgttttgac ggcggcggcc tacgttttgg gtgtgcacaa
    11161 caggctgcat acggggacgt acagactgcc ttcggaagtg tctgcttggg atatcttgca
    11221 gaaaatgcgc ggcggcaggc cggattccgt taccgtgcag attatcgaag gttcgcgttt
    11281 ttcgcatatg aggaaagtca tcgacgcaac gcccgacatc ggacacgaca ccaaaggctg
    11341 gagcaatgaa aaactgatgg cggaagttgc gcccgatgcc ttcagcggca atcctgaagg
    11401 gcagtttttt cccgacagct acgaaatcga tgcgggcggc agcgatttgc agatttacca
    11461 aaccgcctac aaggcgatgc aacgccgcct gaacgaggca tgggcaggca ggcaggacgg
    11521 gctgccttat aaaaaccctt atgaaatgct gattatggcg agcctgatcg aaaaggaaac
    11581 ggggcatgag gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc tgaaaatcgg
    11641 tatgcgcctg caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat acaagggcaa
    11701 aatccgtaaa gccgacctgc gccgcgacac gccgtacaac acctatacgc gcggcggcct
    11761 gccgccaacc ccgattgcgc tgcccggcaa ggcggcactc gatgccgccg cccatccgtc
    11821 cggcgaaaaa tacctgtatt tcgtgtccaa aatggacggc acgggcttga gccagttcag
    11881 ccatgatttg accgaacaca acgccgccgt ccgcaaatat attttgaaaa aataaaccat
    11941 gccgtctgaa aagtttgtgt tttcagacgg cataccctta ccggaactgc aagcatgaaa
    12001 ccgcaattca tcactttgga cggcatagac ggcgccggaa aatccaccaa ccttgccgtc
    12061 atcaaggcat ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga gccgggcgga
    12121 acgccggtcg gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa agccggtttg
    12181 cgtgcggaaa cactgatgat gttcgccgcg cgtatgcagc acatcgagga agtcatcctg
    12241 cccgcgcttt cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc gaccttcgcc
    12301 tatcagggcg gcgggcgggg gatgccgtct gaagacattg aaattttgga acattgggtg
    12361 cagggcggtt tgcgccccga tttgaccctg ctgttggatg tgccgctgga agtatcgatg
    12421 gcgcgtatcg gacaggcgcg cgagaaagac cggttcgagc aggagcaggc ggatttcttt
    12481 atgcgtgtgc gcggcgttta tctcgaccga gccgccgcct gtcccgaacg gtacgccgtt
    12541 atcgacagta accgcagctt ggatgaagtc agaaacagca tagaaaaagt gttggacgga
    12601 catttcggct gttgatgcgg caaatattga aacaagcgca tccgcccgcg ccgaaaacca
    12661 aacggcagtg ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat gatagaatta
    12721 cgctcggtta caaggcagga tgcgtcggca atattaacga accgcccgta acatgatgac
    12781 ccgaaagcgt ttcggacagt tcgattcaaa tctttttctc gcaacaggat tgacacatgg
    12841 aaaactcatt gaaagaagcc gccctcaagt tccacgaatt acccgttccg ggcaaaattt
    12901 ccgttacccc gaccaaatct ctggcgaccg acaaagattt ggcgttggcg tactctccgg
    12961 gcgtagccgc cccctgtgtg gaaatccatg ccgatccgca aaatgcctac aaatacactg
    13021 ccaaaggcaa cttggtcgcc gtcatttcca acggtacggc cgttttgggc ttgggcgaca
    13081 tcggcgcgct ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc aaaaaattcg
    13141 ccggtgtgga cgtgttcgac atcgaaatcg acgaaaaaga cccgcaaaaa ctggtggaca
    13201 tcatcgccgc tttggagccg accttcggcg gcatcaacct cgaagacatc aaagcacccg
    13261 agtgtttcta catcgaacgc gaattacgca aacgctgcaa aatccccgta tttcatgatg
    13321 accagcacgg cacggccatc attaccgccg ccgccgtatt gaacgccctg cgttataccg
    13381 gccgtaaaat tgaagaagcg actttggtgt gctccggcgc aggtgccgcc gcgattgcct
    13441 gcctgaacca actgctggat ttgggcttga aacgtgaaaa cgtaaccgtt tgcgactcca
    13501 aaggcgtgat ttaccaaacc cgcgaagaca aagaccgcat ggacgaatcc aaaaaattct
    13561 acgccgttga agacaacggc cggcgcgtac ttgccgatgc ggttaaaggc aaagacatct
    13621 tcttgggcct ctccggcgca aacctgctga cgcctgaaat gttgaacacg atgaacgaaa
    13681 aacccatcgt gttcgccatg gccaacccga atccggaaat cctgtcgccg ctggcgaaag
    13741 aaacccgtcc ggacgtggtt atcggtaccg gccgctccga cttcccgaac caagtgaaca
    13801 acgtattgtg cttcccgttc atcttccggg gtgcgttgga tgtcggcgca accaccatca
    13861 acgaagaaat gaaacgcgcc tgcgtgtatg ctttggcaga tttggcgatg gaagaagtaa
    13921 ccgaagaagt ggttgccgct tacggtaaga aattcgaatt cggcgcggaa tacctgattc
    13981 ctactccgtt cgattcccgc ctgctgcctc gcgttgctac ggctgccgcc aaagcagcga
    14041 tggaaagcgg cgtggcaacc cgtccgattg cagatttgga agcttacgct gccaagttgg
    14101 gcgaatggaa gctgtaagcc gtttgtggtt aaaatgccgt ctgaactgtt ttcaggcggc
    14161 attttgttgt cagattgata aatgaaagat actggaaaat gaaagagatg aaacctgtcc
    14221 gttatcatat tggcgatata cccgaaactt caaaacaaac cgcccccggt catgacgaca
    14281 gggcagtggg tgttgacgat gattgttttc atgattcctt tggtcaatat cgttatgttt
    14341 tttgtttggg cgttcggcag aggcaacccc aaccgtgcca atttctgtaa agcactgttc
    14401 ttatttacct tgttggttcg cttatcggtt tgattttcat attgcttata ggtgggtctg
    14461 catcgggtac gcattattaa tgtgccggct gattctgctt cgaagatttg tatcgaatat
    14521 gccaaatttt tttaaatttc ataccgttat cgaacggcat tggcaaaaac cttatccggt
    14581 tttgtctttt ctgctcaagc cgctctccgg gctgtttgcc aaaattgcgg caaaatggcg
    14641 ggcggatttt ttatcgggaa aacggcaaag cgaaaagctg tccgtgcctg tggtcgtggt
    14701 cggcaacatc cacgcgggcg ggacgggcaa aacgccgatt gccgccgcgc tggtgtcggg
    14761 cttgcaggaa aaaggtgtca aggtcggcat catcagccga ggctacgggc gcaagagcaa
    14821 ggcggtttat gtattgaatg ccgcgagccg tgcggaagat gcgggcgacg agcctttgct
    14881 gctgttccgt aaaacaggtg cgccgacggc agtgggcagc agccgtgtgg aggcaggcag
    14941 ggcgttgctg gcagcgcatc cggagcttga attgattgtg gcggacgacg gtttgcagca
    15001 ttacgccctg caacgcgatg tggaaatcgc ggtgtttccg gcggcggata cggggcgtac
    15061 ggatttggac ttgctgccca acggcaattt gcgcgaacct ttgtcgagat tggaaagcgt
    15121 ggatgcggtg gtggtcggcg gcagggcggc ggatggtttt atgccgtctg aacatttgtt
    15181 cggcagccgt attgaggcgg gtgcggttta ccgtttgaac cgtccgtctg aaaaactgga
    15241 tatttcgaca ttgtcaggga agcgcgtcgc agcggttgcc ggtatcgcca gaccgcagcg
    15301 tttcttcgat actctgacac acatgggcat ccgccttgac caaacggttg ctctgcccga
    15361 ccatgccgat attttcaatc gggatttgcc gcctgccgat gtggtgctgg taactgaaaa
    15421 agatgcggtc aaattttcag acggcatttg caccgataat gtttgggtgc tgccggtttg
    15481 tgcgataatc gaacctgatt tggcggagtt tgtgctggag cggttggagg gtgtaccgaa
    15541 ggccgtttga aagcgcggtt tggacgaagt gataacggac tggaataaga acgccctacg
    15601 ccgtcattcc cgcgcaggcg ggaatctaag tctcgaattt tcaggaatgc ctaggagact
    15661 tcagaaatcc caaatctccg gatttccact tgggcaggaa tgagaaagcc ggttgtattt
    15721 tttatctgca ttaatcattc gttaaaggag tggatatgaa gctgaaaacc ttgttattgc
    15781 ccttcgccgc actggcattg tgtgccaacg catttgccgc cccgcccggc gacgcgtcgt
    15841 tggcacgttg gctggatacg cagaatttcg accgggatat agaaaaaaat atgattgaag
    15901 gctttaatgc cggatttaaa ccgtatgcgg acaaagccct tgccgaaatg ccggaagcga
    15961 aaaaagatca ggcggcagaa gcctttaatc gttatcgtga gaatgttttg aaagatttga
    16021 ttacgcccga agtgaaacag gctgtccgca ataccttatt gaagaatgcc cgtgaaatat
    16081 acacgcaaga agaaattgac ggcatgattg ccttttacgg ttcgcctgtc ggtcagtccg
    16141 tcgttgccaa aaatccgcgc ttaatcaaga aatcgatgag tgaaatagcg gtatcttgga
    16201 ctgcattgtc agggaaaatc gcgcgacatc atctgcccga gtttacggaa gagttacggc
    16261 gcatcatctg cggcggtata gtggattaaa tttaaatcag gacaaggcga cgaagccgca
    16321 gaccgtacaa atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact
    16381 ataaaaatcc cgatgcgggc tgcaagcagg ccggacagct tgggaaaagg tatcagaagt
    16441 aaataatagc cgcctgaaat attgcggagg gcatccgatt gattaaacca tcaaacccga
    16501 aagcaacctt atggaaaaaa aattcttaga catcctcgtc tgccccgtta ccaaaggcag
    16561 gctggaatat catcaggaca aacaggaatt gtggagccgt caggcgaagc ttgcctatcc
    16621 gattaaagac ggcattccct atatgctgga aaacgaagca cgagcgttga gcgaagagga
    16681 actcaaagca tgaccgaatt cgtcgtattg attccggcgc ggctggattc gtcgcgcctg
    16741 cccggaaaag ccttggcgga cattcacggc aaaccgatgg tcgtgcgcgt tgccgaacag
    16801 gcggcaaaaa gcaaagccgc gcgcgtcgtc gtcgccaccg atcatcccga cattcagacg
    16861 gcctgtcagg cgcacggtat cgaagtcgtc atgacttcaa accggcacga aagcggcacg
    16921 acgcgccttg ccgaagccgc cgccgcgctg aagctgccgc cgcatttgat tgttgtgaac
    16981 gtacagggcg acgagccgct gattgccccc gaactcatcg accgcaccgc cgaagtactc
    17041 gtcgaaaaca acgtccaaat ggcgaccgcc ggccacgaat tgcacgattt cgacgaattg
    17101 atgaatccca atgccgtcaa agtcgtcctc gacaaaaacg gcaacgccat ctacttcagc
    17161 cgtgccccga ttccctatcc gcgcgatgcg atgcgtgccg gaaaacgcga aatgccgtct
    17221 gaaaccgccg tcctacgcca tatcggcatt tacgcctacc gcgccggctt cctgcaacgc
    17281 tatgccgaaa tgagcgtctc gccgctggaa accatcgaat cgctggaaca actgcgcgtc
    17341 ctgtggcacg gctaccccat cgccgtcgaa accgccaaag aagcccccgc cgccggtgtg
    17401 gatacgcagg aagatttgga cagggttcgc gctgtatttc agaccgtata aaacaggttc
    17461 aaagggaaaa gatatgcagc aacatattga aaagtggcaa cacttgagcc gtgaagaaca
    17521 gaaaatcctt gccgaagtat ggggtctcgt gcaaaacgat gatcaggagg ttcactatga
    17581 aatgctcaaa ttgaacgcac ctgatgaagt cagcggtgaa ttttggttca gaatggcaga
    17641 aacactcagc accctgccgc ccaatcgttc cctcgacctt agaatgaacg gcggcaggct
    17701 gtcgaccgcc gtatccatcc tttccgccat gattgaagac aatcccgaca taccgcagct
    17761 ttgggcgtaa aaaattaccg ccctcaatta tttggtacac ggacacaaag cccgtgccga
    17821 cggtttggta caacagcccg acaaagcggc agaagccaac gaggaggaat acctgaccaa
    17881 agccctgtcg caaaacctgc tgtcaacatt ggatgccgca cttgcacgtt ttcctgaaga
    17941 cgcgtggttt caggaaatca aacaggatgc acaaaagcat tttgcttgag gacgtggcgg
    18001 tcaggaatat ttccattcag gaagaaaaga agtgcctaat tgggtataat cagggtaaat
    18061 cttattttat ttcagaagat taatatttgt tttccgtttt tccttgacgg tatcggaaaa
    18121 gttgattata gttacagctt ccttaggagt aatggctgag aggctgaagg cacttccctg
    18181 ctaaggaagc atgtgggatc aacctgcatc gagggttcga atccctctta ctccgccaga
    18241 taaaaaatag acgctgtgtt ttacagcgtc tattttttta tgcaatttta tagcgggttg
    18301 gcgcaaaccc ggtatggtat tgccctgtct tgattctgaa ttttgttaat ccgagatgtt
    18361 tgccgtttat ttttgcctcg ttcaaacggc ggctctgatc tgcgcggttt ctgtttgccg
    18421 tattcgccta tccgtaccgc aaatgttata ctgggaaaaa ttcaccgatt gtattttacg
    18481 gcgtatttgc cgataggatg gaagagacaa atgagcagaa tccggcaggc ttttgccgct
    18541 ttggatggcg gaaaggcatt gattccctat attgcggtgg gcgaccccga tattcggaca
    18601 actttggcat tgatgcacgg catggttgca agcggtgcgg atattttgga gttgggcgtg
    18661 ccgttttccg atccgatggc ggacgggccg gttattcagc gtgcggcgga gcgggcgttg
    18721 gcaaacggga tttcgctgcg cgatgtcttg gatgtcgtca gaaaattccg tgaaaccgac
    18781 acgcaaacgc cggttgtttt gatgggatat ttgaatccca tacataagat gggttatcgg
    18841 gagtttgctc aggaagccgc aaaggcgggg gtggacggcg tgttgacggt ggattccccc
    18901 atcgaaacca tcgattcttt atatcgcgag ctgaaggata acgaggtcga ctgtattttc
    18961 ctgattgcgc cgacgacgac ggaagaccgt attaaaacca ttgccgagct ggcaggcgga
    19021 tttgtctatt atgtttcgct caagggcgta acgggcgcgg caagtttgga tacggatgag
    19081 gtttcgcgta aaatagagta tttgcgtcag tatatcgata ttcccatcgg tgtcggtttc
    19141 ggcatcagca atgcggaaag cgcgcgcaaa atcggccggg ttgccgccgc gatcattgtc
    19201 ggcagccgga ttgtgaaaga gattgaaaac aatgcgggca acgaggctgc cgccgtcggt
    19261 gctttggtca aagagttaaa ggatgccgtg cgctgattct gtcgcgcatt ctaaatgttt
    19321 taggagttgt ccatgagctg gttagataaa atcctgccac ccaaaatcaa gaatcgtggg
    19381 aaagacggtt cttccaatgt tcccgagggt ttgtggcgca aatgcccgtc ttgttcggca
    19441 accgtttatt cgaccgagtt gcagcagaac aatcaggtct gcccgaaatg caaccaccac
    19501 aatccgttat cggcgcgcca gcgtttgaac ctgcttttgg atgaggaagg cagggaggaa
    19561 gttgcgggta atgtcaaacc gaccgatcct ttgaagttta aagacagcaa aaaatatccg
    19621 gaccgtttga gtgcggcacg caagctgacc ggggaagatg acgcgctggt ggtgatgaag
    19681 gggatgatga acggtctgcc cgtcgtcgtt gccgcgtttg aattccgctt tatcggcggt
    19741 tcgatgggtt cggttgtggg cgagcgtttc gtacagggtg tccgccgtgc ggttgccgat
    19801 aattgtccgt ttgtctgtgt ggcggcttcc ggcggcgcgc gtatgcagga gggtgtaaac
    19861 tcgctgatgc agatgacgaa aaccagtgcc gcgctgcatt tgctgacgga aaaacgcctg
    19921 ccgtttatat cggtgttgac cgatccgact atgggcggcg tatctgccag cttcgcattt
    19981 ttaggcgatg tcgtgcttgc cgaaccgaac gcgctgatcg gttttgccgg tccgcgcgtg
    20041 attgagcaga cggtgcgcga aacgctgccg gaaggcttcc aacgcgccga gtttctgctg
    20101 gaaaaaggcg cgatcgacca gattgtcgac cgccgcgata tgaagcggcg catcagtgat
    20161 ttgattacgc tgttgtgccg tcaggacaaa gtttccgcca cctgatggct gatgaatcga
    20221 atgccgtctg aaaccgatgt ttcagacggc atttttgtgt ctggttattt gtcgtgcggc
    20281 ttcatcgacg gggcatagcg tccggcacgt tctttcaggc gttgtaccaa accttgcgtg
    20341 tcggcgggta cgccgccctc gcagaatgcc tgatacagga cggcgcgcaa tgcgtcgttg
    20401 cgcccaagtg tgccgcctat cggtttccat tcggcgtttc ggggctgtat ccagcggctg
    20461 ttgaccgtat cgccgtatcc gaatactttg taggaggaaa gtttgccgtt gccgaacccg
    20521 atggaggagc gggcgcagaa tatgccttcg gcagtcaggt tgtcgtagcc tttgtcggag
    20581 cggatattga gaatgtagcg gatgctgccg tcgggcgcgg gcataatttg caggctgtcg
    20641 agcaggattt tcggctgttt gccgtaattt tcatccacat aaatgtcaaa ccagccgtcc
    20701 gagtgcgcat cgggcagagg cggcagttcg gcggtatgtt ctttaaattc gcgggcggcg
    20761 gcttcttcgg gcgtttcgcg gtagcgggtg ttgatcggcg tgtctttttg gctgaagccg
    20821 gcggcaaggg acgtgccgac agtcagggtc agaatcagaa ggatggcgcg gcgcataagt
    20881 ttctccaaat tgaaaacggc tttattttat ggtttggcgg gaagggctgc aagcaatcgg
    20941 ggtataatct gacctgattt tcattttaaa gcggtgtcga accatgaaca gcgaaacttt
    21001 agacgtaacc ggattgaaat gtcccctgcc gattttgcgg gcaaaaaagg ctttggcgca
    21061 aatgcggcag ggcgaggtat tgaccgttct ggcgaccgac ggcggcgcgc cgggggactt
    21121 tgaggctttt tgccgccaaa ccggtcatgt gctgttggat tcttccgaac aggacggcgt
    21181 gttcacactg gtcgtcaagc acaaataaat gccgtctgaa atgcagatgt cctgattgat
    21241 gttattgctg tcggcatttg tgcttgctgc gtgtaatgcc caaaaccggc aatccggtgt
    21301 acggcatcag gattttgcac gcgtagccgt cgatttgttt cggagtgcct gcgtcctgac
    21361 tcaaggtagt ttcgaaccgg tttccaagtt tgccgctgtc ggacattttg tccctgtcgg
    21421 aaaggaagac ctgtcccgtc tgccgcccgc tgttgcagaa cccgatgtac aggcgttgtg
    21481 gacgcttgaa cgcgatggcg gcatttatta tttgagcctg acgcgcgaca gttgcagtgt
    21541 gaaagccgag cgggcagaca gtgccgtgct tttggaacat tttgacggac ttgtccgaca
    21601 gccgccgaaa aatgccaa
//
LOCUS       DAXFHF010000035        21390 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXFHF010000035 DAXFHF010000000
VERSION     DAXFHF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21390
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.035"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..545)
                     /locus_tag="ACFIRM_001204"
     CDS             complement(<1..545)
                     /locus_tag="ACFIRM_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3028839.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLV"
     gene            732..1004
                     /locus_tag="ACFIRM_001205"
     CDS             732..1004
                     /locus_tag="ACFIRM_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028840.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            1212..3140
                     /gene="dnaK"
                     /locus_tag="ACFIRM_001206"
     CDS             1212..3140
                     /gene="dnaK"
                     /locus_tag="ACFIRM_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC3028841.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            3363..5283
                     /locus_tag="ACFIRM_001207"
                     /pseudo
     CDS             3363..5283
                     /locus_tag="ACFIRM_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            5340..6317
                     /locus_tag="ACFIRM_001208"
     CDS             5340..6317
                     /locus_tag="ACFIRM_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC3028842.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFDT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            6654..6791
                     /locus_tag="ACFIRM_001209"
     CDS             6654..6791
                     /locus_tag="ACFIRM_001209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028843.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            6870..7640
                     /locus_tag="ACFIRM_001210"
                     /pseudo
     CDS             6870..7640
                     /locus_tag="ACFIRM_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            7667..8359
                     /locus_tag="ACFIRM_001211"
     CDS             7667..8359
                     /locus_tag="ACFIRM_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3028844.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            8551..9165
                     /locus_tag="ACFIRM_001212"
     CDS             8551..9165
                     /locus_tag="ACFIRM_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC3028845.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            9461..10557
                     /locus_tag="ACFIRM_001213"
                     /pseudo
     CDS             9461..10557
                     /locus_tag="ACFIRM_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(10604..12793)
                     /locus_tag="ACFIRM_001214"
     CDS             complement(10604..12793)
                     /locus_tag="ACFIRM_001214"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC3028846.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTDQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRALLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            12933..13718
                     /locus_tag="ACFIRM_001215"
     CDS             12933..13718
                     /locus_tag="ACFIRM_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC3028847.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(13817..15751)
                     /locus_tag="ACFIRM_001216"
     CDS             complement(13817..15751)
                     /locus_tag="ACFIRM_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC3028848.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(15820..16995)
                     /locus_tag="ACFIRM_001217"
     CDS             complement(15820..16995)
                     /locus_tag="ACFIRM_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC3028849.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLNMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQTALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPR"
     gene            17221..17610
                     /locus_tag="ACFIRM_001218"
     CDS             17221..17610
                     /locus_tag="ACFIRM_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3028850.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFQTYEKNKLKQNEYFKITLIKTSPDHFTLKADPNPMTNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(17704..21189)
                     /gene="smc"
                     /locus_tag="ACFIRM_001219"
     CDS             complement(17704..21189)
                     /gene="smc"
                     /locus_tag="ACFIRM_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC3028851.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIAAELENLAPGLSAAEAAFKQTE
                     AAVCSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPHGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
BASE COUNT         5011 a         5821 c         5485 g         5073 t
ORIGIN      
        1 ttgaccaaca gattttcatt aatccgcaac acatacagtc cgtctctcgg cgtattttcc
       61 ccatgattga ccaaaatcga atctccgtca ttcaaaaccc cctccatcga atcccccttg
      121 acggaaatta cagacaggtt tttcgtatct cgggtaacat aattctcaat ccaatgccgt
      181 ctgaacgcca ttgtaaatac cggttcctca tgaccgacaa actgcccgta tcccgcagcc
      241 gcccgaatat catatctcgg cacaaagaca aactcgtccg tatcaacttc atttcccaag
      301 gtatcgtaag caagggattt ttttggggct tcatccggaa acggattacc ctcccctgtc
      361 agcagccaat cgatactaca ccccttcaac tgcttaattt ttttcaatgt ttcagacttc
      421 ggcagaccgc cttcattcca tattctgctg aagcccgcaa tcgtcatttc aatatcggat
      481 gcgatttttg cctgcctcgc ttcacttttc cataaaaaaa ccagtctgtc tttaaaggtt
      541 tccataaata tccttccgct ccccttcatt caatcatcta aagtaaacat gaaatatatt
      601 ttacattagc tatcccaaaa acggaataac tatgataaac taaatttaaa ttatgatttt
      661 tcttatccaa tacataattt ttcagatttt ccataggttt gatcccgaat tgacagaaaa
      721 aggatagaaa aatgaagaaa accagcaaat atcttatcta tactgcggca tttacctcat
      781 tctgctttgc cttccaagaa aaccgttctg aagccaaaca gcccgacatc actttatccg
      841 catccctgtg cgaacaattc aacatgctga acgccaaaga tatggatgca gaacaagtct
      901 ccctttccaa agaatgcgac atcatcgagt cttcacacga ctgggaaaaa gagtacggca
      961 acttgaacga acaggaaatg ctcgccggcg tcgtctatga ataaacctgc ctgccatttg
     1021 aaacattatg cttgaatgca ttggagccaa atgtattaaa tcaaatataa aaccaatata
     1081 ttcataaagt tatatactta tagccatgct gtagcttgaa acagcccgcc gcccgcccta
     1141 tttacagccc atcgggacaa aatgttccaa catatttctc aaaataagca aaatcaaata
     1201 ggagtatcca catggcaaaa gtaatcggta tcgacttagg tacaaccaac tcttgtttgg
     1261 ccatttccga aaacggtcaa accaaagtca tcgaaaacgc agaaggcgca cgcaccacgc
     1321 cgtccattat cgcttatttg gacggcggcg aaatcctcgt cggcgcgcct gccaaacgcc
     1381 aagcggtaac caacgccaaa aacactattt acgccgccaa acgtttgatc ggtcacaaat
     1441 ttgaagacaa agaagtccaa cgcgacatcg aatctatgcc tttcgaaatc atcaaagccg
     1501 acaacggcga cgcatgggta aaagcacaag gcaaagagct gtctcctcct caaatttccg
     1561 cagaagtcct gcgtaaaatg aaagaagccg ccgaagctta cttgggcgaa aaagtaaccg
     1621 aagccgtgat taccgtccct gcctacttca acgacagcca acgtcaagcc accaaagacg
     1681 caggccgtat cgccggtttg gacgtaaaac gcatcatcaa cgagccgacc gcagccgctt
     1741 tggcattcgg tatggacaaa ggcgacaaca aagaccgcaa aatagccgta tatgacttgg
     1801 gcggcggtac cttcgatatt tccatcatcg aaatcgccaa cctcgacggc gacaaacaat
     1861 ttgaagtatt ggctaccaac ggcgatactt tcttgggcgg tgaagacttc gaccaacgct
     1921 tgattgacta catcattgac gagtttaaaa aagaacaagg cattgatttg aaacaagacg
     1981 taatggctct gcaacgtctg aaagaagctg ccgaaaaagc caaaatcgaa ttgtccagcg
     2041 gccagcaaac cgaaatcaac ctgccgtaca ttaccatgga cgcaaccggc ccgaaacact
     2101 tggcaatgaa aattacccgc gccaaattcg agagcctggt tgaagacctg attgcccgct
     2161 ctatcgagcc ttgccgcacc gcattgaaag atgccggctt gagcaccggc gacatcgacg
     2221 acgtgatttt ggtcggcggt cagtcccgta tgccgaaagt acaagaagcc gttaaagact
     2281 tcttcggcaa agaaccgcgc aaagacgtga accctgacga agccgttgcc gtaggtgcag
     2341 cgattcaagg cgaagtattg agcggcggcc gcagcgacgt attgctgctg gacgtaaccc
     2401 ctctgtcctt gggtatcgaa accatgggtg gtgtgatgac caagctgatt caaaagaaca
     2461 ctactattcc gactaaagcg tctcaagtgt tctctactgc cgaagacaac caaagcgcag
     2521 taaccatcca cgtactgcaa ggcgaacgcg aacgcgcttc tgccaacaaa tctttaggtc
     2581 aattcaactt gggcgacatc gcacccgcac cacgcggtat gccgcaaatt gaagttactt
     2641 tcgacatcga cgccaatggt atcttgcacg tttccgccaa agacaaaggc actggcaaag
     2701 cggccaacat taccatccaa ggttcttcag gtttgagcga agaagaaatc gaacgcatgg
     2761 tgaaagatgc cgaagccaat gccgaggaag ataaaaagct gactgaattg gtcgcttccc
     2821 gcaaccaagc cgaagccctg attcactccg tgaaaaaatc tttggcggac tacggcgaca
     2881 aactcgacgc tgccgagaaa gaaaaaatcg aagccgcgct gaaagaagcc gaagaagccg
     2941 tgaaaggcga cgacaaaacc gccatcgatg ccaaagccga agcactgggt acagccagcc
     3001 aaaaactggg cgaaatggtt tacgcgcaag cgcaagccga agcccaagcc ggcgagggcg
     3061 cacaagccaa tgcttctgca aagaaagacg atgatgtcgt agatgccgac tttgaagaag
     3121 taaaagacga caaaaaataa ttgatgccgt ctgaaaaaaa cgcgaaccat tcggttcgcg
     3181 tttttttcaa ttgagataaa agacaatagc atgacagaga ttccaaattc atctaccgtg
     3241 atattcccaa gcccaagttc caactgttgc atcggttatc tggaaatttt tcatatcatt
     3301 ttcttattta cttaaatttt ttaataagat aataaataat aattattatc atgagctaag
     3361 aaatgaaact aaatactctc acatgggctt tgatgaccgt tttttccgtt gcgccatctt
     3421 gggcagaaca accggcaaat actgaagaaa tacaacccgt caaaaccttc tccccgccca
     3481 aaccgattgc accgaccgcc gcacaaggct atttccccga aaaccaattc gaccgctccg
     3541 accgcagcga ttattacttt gttaccgaaa acatagacca agccttccgt ccgctgaagg
     3601 caaacagcag tttttacggc aaaagctttt acaactccgt taccgcgcaa gcactcgggg
     3661 cgaaggtata cggcgtagcc aacctcaatc gcaccaaggc aaacggctac aaagatggcg
     3721 gtgggcgcga caccgattgg aaatacagcc gcttcaatca ggctttggta ctcggtttcg
     3781 tgccgtctga aaatcaagaa taccgcctca cttatctgca cgacgacatc aacaacgacc
     3841 gccagccgca ggtcgtcaac gacgcattgg acaccgaacg ccacatctcc aaactcaacg
     3901 tgcgttgggg caatgccgat ttgagcaata cggtcagcgc ggaagcaggc gtcatcaaac
     3961 tcaaacgcca tgccgacaat tactccttgc gaccaaacaa cacgccgcag caagtgttcg
     4021 tagaacttga ccgcaaagtg tatgatttct cgctcaaaca cgatgcggat ttcggcaaat
     4081 tccacaacac ggcagccgtc agctaccgca acgacagcca aaacggcgaa cgcaacaccc
     4141 acactgccat gtgcgatttc ctcaacggct accgtttcgc cgatgtgcac atcgaccgct
     4201 ggtgcattgc cgatacctta tcttacaagt ttgacgaccg acacaaactg ggcttgggct
     4261 taagctacga actcaacgaa gcggacattc gcaaaaatac ggcacagcct gaaaacccaa
     4321 taaaacccgg tttccccttc gcctcatcac aacaaatctg gaaaacccat tacggctacg
     4381 acttcaacgg caaagtgcgc cgtcatgctt tgtcgggcga actcaaatac gatttcacgc
     4441 cgtctgaaac gcaaaaatac agcgtttccc tcgcgcattt ggaacgcatc ggcgacaaca
     4501 ccgaacgctt caactcgctt gctgccatcg tgcaaaaccg tatgagcggc atgctgatga
     4561 accaaaatcc agccgccgcc attgcaggca atccccctgc tgaaaactga aaaacacaac
     4621 cgcatcaggc tcaccgccga cagccgcaac gactactaca acggctacat gaactcgctc
     4681 gcaggcgcgg gctggaacgt gggcggcacg cttgtggcgg acaaagtcaa agacctgatt
     4741 atttttgacc gcgcacacgg acaaagcggc acagcttcca aagacggcgg catcatcaca
     4801 cgcaacgtgg acgcacgatt gtttaccgcg caggcctacg cgcgttacaa cttcaatccg
     4861 cattgggcgg caggcatcaa agccgcctac aactacggac acaacgaaac cgacggcagg
     4921 ccgccctatc aaatccgtcc gtttgaagcc gccgtccaag ccgactacaa aaactacttt
     4981 gcccacggca gctacaacat cggcgcggca acacgctttg tcgccaaaca aacgcgcggt
     5041 gattttgata tggcaagcgg tctgggcata gacaaacgcg aagccgccaa aggctttacc
     5101 gttgccgacg tgtacgcagg cgtaaacatc aaagacaaat acggcttgcg cttgggcgtg
     5161 aacaacgtat tcaacaaaaa atacgtcgaa tacatcagcg gcgaccacgt cctcgcccta
     5221 tcccccagcg tagtgtatgc accgggcagg acatattggt tgagtttgca tgcggcattt
     5281 taaaaccatg ccgtctgaaa aatacctgca ttggcttatt aagaattagg agataaaaaa
     5341 tggatatgaa aagacgcgat ttcttaaaaa tgaccgccgc gctggcagcc gcaggcgttt
     5401 cgccttctct gcttactgcc ggtaaagagc aatttaccgt gtacggcgca ccggcaatgc
     5461 ccagcgttac cattgccgta acggcgttgc aaggtaaact ggcgaaacag gcggacgtgt
     5521 cgctgaaaat ctggcgttcg cccgaccaac tgcgcgcggg cgtggcaagc gggcaattta
     5581 aagtgatgat gagtccgagc aatgtcggcg taaacctgcg caaccaaggg cagaaagtcg
     5641 gcatggtgaa tattttgacc aacggcatca cgcagttggt ctgcaaaggc agcgcgattg
     5701 cctcgccgca ggatttggtc ggcaaaaaaa tcctcgtgcc gtttaaaaac gatatgcccg
     5761 acatcgtgct gcaagccttg ttgaaaaaac tgaaaatcga cgcacacaaa gtcagcatta
     5821 cctacgctgc cacaccgccc gaagcagtcg gactatttcc aagtaaaggt taccatgccg
     5881 tcatcctgcc cgaaccgatg gcaaccgcca gcctactgaa aggcaaaacc ataggtataa
     5941 acgtcgtgca cggttttgat ttggtaaaag catgggggca ggcgtttgac accaaaccgc
     6001 tgattccgat ggcaggtatc atcgccaacg aagaatattt ccacgcacac aaggcgcagt
     6061 tcgacatctt ccatcaagat ttgaaaaacg cgctcaactg gatattggcc aaccgccaaa
     6121 acgcggcgaa aatcggcaaa aactacctac ccgcccccga acccgcccta gtcatgggct
     6181 tggacggcgc acggctgacg gtaagcaaag gcagcgaagt gaaaaacgag attttgaagt
     6241 tttacgaaat cctgatgcag ttcaacccgg aacttttggg cggcaagctg ccggataacg
     6301 ggttcttctt ggcttaaagg gaaagaggcc gtctgaaaag ggtagggtgg ttgtgaatac
     6361 ctgatttatc atttttcaga cggcctatta aggcattgtc agcggcgtgg gtcggattct
     6421 tgaatccgac atttcggcta aacggggctg tcggatacaa gtatccgacc tactcttttc
     6481 agacggccta tcagataata gttcgaacag tacacaaaga gccgtcggat tgcctgaaat
     6541 gttgggtcat gacccaacct acgcttactg gggaaacggg atcgaagtgt gaggtatgtg
     6601 gcggggttgt ttaataatga aattttataa ctatttgata gggtggtaat gctatgaatg
     6661 aagataaata tagggaaaat atgacatggg atgaaattga aaatgtagta ggaactttag
     6721 tttctgaaga aagaatgctt aaatccaata gctttttaag tattaaaaat agtaagaata
     6781 ggttggctta atcacaacca taacccaacg aaaatactaa aattccaagc taaggctatc
     6841 ctttccaaac catccccatc aaccaaacca tgattaaaac cgacaaaatc cgcaaaccgc
     6901 agcctgcgct gttttacatc atcgactacc tttggagcgg ctttgccggt ctgagcgtgg
     6961 cgatggtggt ggtggcgttg tgggcgtggg gcagtgccgt gttcggcgag tttatgctgc
     7021 ctgcgccggt agaggtgttt caaaaatctt tggatttatt gaaacatttt caggaaaatg
     7081 aaatcgggat ttcgctgtgg cggtcggtag tgggtatttc ggttgctttg atagcaggat
     7141 tggcggcggg gctggtggcg ggcagtttta agacggcgat ggcgttgctt aagcctgtga
     7201 ttacgatttt gttagcaatg ccgccgatta tttgggtggt gatggcgttg ttttggttcg
     7261 ggttcggcaa tccgagcgtg ttgtttacca tcattgtgtt ggttgcgccg ctgacgtttg
     7321 cgagtgcggc ggtcggaatg gcgagcgtga acaagcagca tgaggagttg tttgacgctt
     7381 ataaattagg ccgtctgaaa aaaatccgct atctgtatat cccgcatctg acgggctatg
     7441 tgatttccag cgtcggcgtg gcggtggcga tgggggtgaa ggcggtgatc atggcggaac
     7501 tcttaggcgc gagcaaaggc gtgggcgcgc ggattgcgga cgcgagggca atgctgtaga
     7561 cttcgacggt aatggcttat gtggtgttgg tcatggtatt tgtgtcgctg tttgaatacc
     7621 tgattaccaa gcctttgtaa attttgttta tgccgtggag gagatgatgc tctgtcttga
     7681 aaacgtgcgt tttgaaattc tccgcgaccc catcgtgcgc gatttcagtt tgaacctgca
     7741 acagggcgaa gtgaaagcct tgttcgggcc gagcggctgt ggcaagacga cggttttacg
     7801 gctgattgcg ggcttggaaa cgccgaaatc gggcacgata cgtaatactt tccacaaaac
     7861 gggttttctg tttcaggaaa accgcctgcc ggaaaacctg accgcgatgc agaatatcgc
     7921 gatttttatg gacaacccag acgaaggcga aatcgtcgcg ctggcggcga aagtcgggct
     7981 gactgcgggc gatttgaaca aatatccgac cgaattgtcc ggcggcatgg cgaaacgggt
     8041 ggcgtttttg cgcctgctgc tgtgcggctg cgacctagcc ttgctggacg aaccgtttgt
     8101 cggtttggac cgcgatttgc gcgatatttt ggttgctatg ctggtggaaa aaatcgagcg
     8161 gcagggcatg gcgtgtatac tggtaacgca cgaccgcttc gaagccgcgc gcctgagcca
     8221 tgaaattatg ctgctttccg ctaagggcat gaacgtgcaa aacgtgataa ccctgcccac
     8281 gccgctgtcc gaacgcgatt cggcttttga agaagtcgtg gtggcaaggg agtttcaggg
     8341 gattcattat tatgagtgat gaggggattt gagtgtctga caaaccttac ggttcgttgc
     8401 cctctcccta accctctccc acggggagag ggttagcaag cctcaggctt gcctctttag
     8461 cgaaagatac gaatctgtta tccgaacgcg attcggtttt tgaaaaagcc gtggtgccaa
     8521 gggggcaggg gattcattat gaggtgcttt atgttttcga ctgtgattac tgctgctgtt
     8581 ttatatattg ctacagcagt agatttgttg gtaatactat taatattttt tgctagagca
     8641 aatactagaa aagaatatcg agatatttat atcggacaat atttaggttc tgtaatttta
     8701 atattagtta gtttatttct agcttttgtt ttgaattatg ttccggaaaa atgggtgttg
     8761 ggtttattag gtttaatacc gatttactta ggtattaaag ttgctattta cgacgattgt
     8821 gagggcgaaa aaagagctaa aaaagaattg gatgaaaaag ggttgtcaaa attagtcggt
     8881 attgttgctt tggttacagt tgctagttgt ggtgcagata atattggact ttttgttcct
     8941 tactttgtga ctttagatct tgtcgactta ttagttactc ttcttgtatt tttaatattg
     9001 atttttgttt tagtatatac agcacaaaga ttggctaata tttcaggtgt tggtgaaatt
     9061 gtagagaagt ttagtcgttg gataatggct gttatttata ttggtttagg gttatttatt
     9121 attattgaaa ataatacaat tcgaacaata atatcaataa tatgaatgac aagggcattt
     9181 cagtatctga caaaccttgc ggttcgtttc cctctcccta accctctccc acaggagagg
     9241 ggatcaggtt gctgaaaatc agagggcgaa ggattgggga tcaaatggaa ggtaaaacct
     9301 gaaaatgttc gggtttgaca gcccgattcc ctctccccgt gggagagggc tagggagagg
     9361 gcaagcaagc cgcaggcttg cctctttggc gaaaaataca accctgtccg cccaataaat
     9421 ccatacccga aagcatcttc atacagaatc aagccaagcg atgaataaat tttttaccca
     9481 ccctatgcgc ccgtttttcg tcggtgcggc agtacttgcc atactcggtg cgttggtgtt
     9541 ttttcatcaa ccccggcgct atcatcctgc accgccaaat tttcttggaa cttatgctgc
     9601 cggctgcata cggcggtttt ttgactaccg ctttgttgga ccggacgggt ttttcaggca
     9661 acctgaaacc tgccgctact ttgatggcgg tgttgttgct tgttgcggct gttttattgc
     9721 cgtttttacc gcaacttgcc gcatttttcg tcgccgccta ttggctggtg ttgctgctgt
     9781 tctgcgcctg gctgatttgg ctcgaccgca acaccgacaa cttcgccctc ttaatgctgc
     9841 tcgccgcatt caccgttttt cagacggcct atgccgtcag cggcgatttg aacttaactg
     9901 cgcgcgcaag tgcatttgaa tatggcggcg gtcatgttcg tatccgtccg cgtcagcgtc
     9961 cttttgggca cggaaaccct gaaagaatgc cgtctgaaag accccgtatt catccccaac
    10021 gttatctata aaaacatcgc catcaccctg ctgctgcacg ccgccgccga actttggctg
    10081 cccgcgcaaa ccgccggttt tactgcgctt gccgtcggct tcatcctgct cgccaagctg
    10141 cgcgaactgc accatcacga actcttacgc aaacactacg tccgcactta ttacctgctc
    10201 cagctctttg ccgccgcagg ttatctgtgg acaggcgcgg cgaaactgca aaacctgccc
    10261 gcctccgcgc ccctgcacct gattaccctc ggcggcatga cgggtggcgt gatgatggtg
    10321 tggctgactg ccggactgtg gcacagcggc tttaccaaac tcgactaccc gaaactctgc
    10381 cgcatcgccg tctccatcct tttcgcctcc gccgtttcgc gcgctgtttt aatgaacgtg
    10441 aatccgatat tcttcatcac cgttcccgag attctgaccg ccgccgtgtt catgctttac
    10501 ctgctgacgt tcgtaccgat ttttcgagcg aacgcgttta cagacgatcc ggaataaaaa
    10561 tgccgtctga aacggttttc agacggcatc ggattgccaa tgatcaagcc tcctgcggat
    10621 cgacatccac cgaccatcgg attttgccgt cgcggttctg ctgcaacacc tgcacccaca
    10681 aactcacggc gcggtgcaaa tcttgtcggg atgttgattc gaggaaaact tgcgcacgtt
    10741 cgcgttcggc aaggcgcacc atcagcatcg gggcggcacc gaaccgggag acgctttcgg
    10801 gcaagagcgg ggcgagggtt tctttggcgg cgttgagaaa ttccatcgca tcggcaacac
    10861 gcggcgcatc ggcacggacg gccgtctgaa aaccgaaagg cggcatggcg aacatttgcc
    10921 gttcgttcaa ttcgttttcg gcaaacacgg cgtagtcctg cgctttgacg gcggcgaaga
    10981 cagggtgttc gggcagttgg gtctgtatca gcaccttgcc gggtttgtcg gcgcgccccg
    11041 ccctgccgga cacttgcatc agctcggcaa acagcctttc cggcgcacgg aaatccgcgc
    11101 tgtacaggct gccgtcggcg ttcaacacga taacgaggtt gagccgcgcg aaatcatggc
    11161 ctttggcaag catctgcgtg ccgaccagaa tgtcgatttc gtcgttggcg atgcggcggt
    11221 acaaatctgc ccagtcgttt ttgtgcgccg tactgtccct atcgacgcgg acgacggctg
    11281 ccttgggcag caaggcgcgc agggtttctt cgacgcgctg cgtaccgtgt ccgacggcgg
    11341 tcaggtcttg gttgccgcag tcgggacatt tgaacgggat gggttcgcgg tggtcgcagt
    11401 ggtggcagcg cagttggcgg gcgcgttggt gcagcaccat tttggcggag cagttcgggc
    11461 agccgaaggt atggccgcag tcgccgcaaa acagcgcagg cgcgaagccg cgccggttaa
    11521 ggtacaccag cgacataccg cccgcttcaa agttctgttt caaaagctgc aaggcttgcg
    11581 gtgagaagcc gttatcgagt ttcagacgac ctatgttgag gatgtccact tgtggcagtt
    11641 gcgcggaagc atgggcgcgt tcggtcagtt gcagcaggcg gtacgcgccg ctttgcgcct
    11701 tgtgccaact ctccaagctg ggcgtggcac tgcccaacac gacgggacag ccgccctgct
    11761 tcgcccgcca caccgccaaa tcgcgggcgt ggtagcgcaa ttcgttgtct tgtttgaacg
    11821 agccgtcgtg ttcctcatcg accacaatca gcccgacatc aggcaaaggc gtaaacaccg
    11881 ccagccgcgt gccgatcacc agcttcgcct gccccaacat cgcgcgcaga taatcctgcg
    11941 tgcgcctgcc tgccgccatc cggctgtgca acacggcggt cggcacgtcg gcaaaacggt
    12001 tttccacccg ctttaaaagc tgcggcgtga ggttgatttc gggcaacaga aacaacacct
    12061 gtcgcccctg cgccaacact ttcgccatcg catcgaaata cacctcggtc ttgccgctgc
    12121 cggtaatgcc gtacagcaga aacgaccgga agcgtcccaa tgccgtctga atttcatcgg
    12181 aagccttctg ctggtcggta tttaatacaa attcagagtg cgaagcctgc ccgcggtacg
    12241 gccttaaaac aggtttcgcc gcttccgtcg tttcaatcca gccctgctcc gcccaatcct
    12301 caatcaattt cgccgcctgc gcgtttgcct gtttcaacgc cgccatcgtc atttcgcccg
    12361 acagcagcgc gtcccacaaa gccgcttttt tgttgaaccg agccggcggc ggcgtttgcg
    12421 ccctgcccgc ttcgttcaaa gcataaaaca acggcggctg cggcatttcc accgcgcgcg
    12481 tttccttcaa accctgcggc aatgcggcaa atacagcctg cccagtcgga tagtggtaat
    12541 aacgcgacgt aaacgccaac aaatcacgcc agctttgaga cagcggcttt tcttccacaa
    12601 agaccgtctg aacgctcaaa atccgcgccg catccatatc gggcgcaata tccgtttccc
    12661 acaccatccc gacaacagtt ttattgcgaa aaggcacaag cacccgcgtt cccggaggaa
    12721 gcggctcgga atgcgaataa gtcaaaaggc cgtctgaaag cggcacgttt acagcgatgc
    12781 gatggtagat catacaggca ggcaaatcat ttttttgcgt attttaacag ccggaagcta
    12841 ttttcagacg ataaattgag cctcggcgca atgcaggcgc aacagaggtt tgatacaatg
    12901 ccacttttcc aacacgctca atcatctttg atatgaaaac caagttaatc aaaatcttga
    12961 ccccctttac cgtcctgccg ctgctggctt gcgggcaaac gcccgtttcc aatgccaacg
    13021 ccgaatccgc cgtcaaagcc gaatccgccg gcaaatccgt tgccgcttct ttgaaagcgc
    13081 gtttggaaaa aacctattcc gcccaagatt tgaaagtgtt gagcgtcagc gaaacaccgg
    13141 tcaaaggcat ttacgaagtc gtcgtcagcg gcaggcagat tatctacacc gatgccgaag
    13201 gcggctatat gttcgtcggc gaactcatca acatcgacac gcgcaaaaac ctgaccgaag
    13261 aacgcgccgc cgatttgaac aaaatcgact tcgcctccct gcctttggac aaagccatca
    13321 aagaagtacg cggcaacggc aagctgaaag tcgccgtctt ctccgacccc gattgtccgt
    13381 tctgcaaacg cttggaacat gagtttgaaa aaatgaccga cgtgacggtt tacagcttta
    13441 tgatgcccat tgccggcctg cacccagatg ccgcgcgcaa ggcgcaaatc ttatggtgtc
    13501 agcccgaccg tgccaaagcg tggacggatt ggatgcgtaa aggcaaattc ccggtcggcg
    13561 gcagcatctg cgacaatccc gtcgcggaaa ccacttcctt gggcgaacag ttcggcttca
    13621 acggcacacc gaccctcgtc ttccccaacg ggcgcaccca aagcggttac agcccgatgc
    13681 cccaactgga ggaaatcatc cgcaaaaacc agcagtaaac ccgcaatgaa aaaggcttgg
    13741 aaccatgttc caagcctttt tcaaatgccg tctgaagcct gtcgcggcag cttcagacgg
    13801 catctttgtt caaacctcaa tcctgcgcca atgcatctat cggattgagt ttggctgcct
    13861 tattggcagg cataaagccg aacgcgatgc cgattccggt cgaacaggcg accgccccga
    13921 taacggatgc cgccgaaatg tccatcggga aatcggttac aaaatgattg aacacgaggc
    13981 tgacggcggc ggacaaacct acgccgacca agcctccgat gatgcagatt aacaccgcct
    14041 caatcaaaaa ctgctgcaaa atattgccgc gccgcgcgcc gattgccatc cgtatgccga
    14101 tttctttggt gcgctcggta acggacacca gcataatgtt catcacaccg atgccgccga
    14161 ctaccaatga aatcagggcg atggaggaaa tcagcagctt catcgtaccg gtggtgcttt
    14221 cgaccatctg cctgatgctg tcgctgttgt tcataaagaa gtcttccgtg ccgtgccgtg
    14281 ctttgagcag ctcggccagc cctttttcgg caacccgggt attggcattg tctttgattt
    14341 tgacggtgat ggagttggtg tggctctcgc ctgtgatttg gtgcatcacc gtcgtatagg
    14401 gcgaccaaag catcagcacg tcggaattgc cgaaagcgtt ttcgtctttt ttcatcacgc
    14461 cgatgacggt caaggggcgt ttcctgaaca aaatggtttt acccaacgga tccgagtccg
    14521 caaagagttt gtctttgaca ttttggtcga tgacgacgac ttgcgcgtct tctttcacat
    14581 cgttctcatc aaacagccgc cccgtttcca gcttcagccc gcgcacgtcg aaatattgtt
    14641 cgcccacacc gtacaaagaa gcggtcaggt cggtattgcg gtaagtcagc gtgccgccgc
    14701 tcgaagtcat gggcgtggcg gaagcaacat agctttgttt ggcgatgatt tttgcgtcgt
    14761 ctatggtcag ggttttgatt ttgccgctgc gcctgtcgcc gaagccgcgc ccggggaaga
    14821 tgctgatggt gttcgtcccc atcgaactga tgtcttcgag gatttttttc tgcgaaccgt
    14881 tgcccagcgc gacgacggag acaaccgaag cgataccgat gatgatgccg agcatggtca
    14941 gaagcgaacg cattttgtgc gccaatactg cttgtaccga cattctgaag gcttcgacaa
    15001 actggtcgta ataaaacgac cacgaggctt tttcctgaat cctcccgaca ttgcttgcgg
    15061 ggatttcggg atttttcgag gtgtcggaaa tgatttcgcc gtcccggatt tcgatgacgc
    15121 ggttggcgtt ggcggcaatg ccggggtcgt gcgtgaccat aatgacggta tgcccggctt
    15181 cgtgcagcct gcggatgatt tccatcacat ttttgccgct ggcggtatcg agtgcgccgg
    15241 tcggctcgtc ggcgaagatg atttctccgc cgttcatcag ggcgcgggcg atggagacgc
    15301 gctgctgctg tccgcccgag agttcgccgg gcttgttgcc ctctttactt gccaaaccca
    15361 aatcctgcaa gagtttgtcc gcccgcgcgg aacgctcttt gcctcccata cccatataca
    15421 cggctggcag cgcgacgttg tcccttgcgg tcagcgagct taagaggttg tagcgttgga
    15481 agatgaaacc gaagcgttcg cgccgcaatg ccgccagttc gtcaggctgc attttggcag
    15541 tttcgatgcc gtcgattcgg tacgaaccgg aaccggcggt atccaaacaa ccgagtatgt
    15601 tcatcagcgt ggacttgccc gaaccggact gcccgatgat ggcgacaaaa tcacccttct
    15661 ctatcgacag gctgatgtct ttcaaaatat ggacgcggtt ctcgccgctg ccgaaatagc
    15721 ggttgatgtt tttacattcg atcaagctca taatattccg taaatcaata acaaatgccg
    15781 tctgaaaccg tttccgtgtt cagacggcat attcgtttat cagcgcggcg ggccgcctag
    15841 ggcgcgttcg ccgctttcct gttgctcggc ggcggttatt tcggagatga ccactttgtc
    15901 cccctctttc aacccgcttt tcacttcggt attcatactg tctctcatac cggtccggat
    15961 ttcgcgttcc gccgccttgc cgtccgcacc caacacgcgt acgaacgcct tgccgccgcg
    16021 atttttcacg gtcagcgacg gaataagcaa cacatttttc acaccgtcga tttcaaccgt
    16081 attctgcgtc gtcatccccg tggcgagttt gccgtccgga ttcggcacaa acgaacgggc
    16141 ataataatag accgcattgg aagccgtatc cgtactgctg ttgtagccgc ccgacgacat
    16201 cgtggtcagc ccggggtcga cgctgtcgag cttcgcctta atcggcgtat ccggttcgga
    16261 caaaatcgta aacgaaatat cctgccccgc cttcaccttg gtaatatcgc cctcggcaat
    16321 ctgcattttg ttcaacatca tatccagatt cgccaattgg acaatcgtcg gcgtagactg
    16381 cgccgcgttc acagtctgcc cctcttccac gggaatcgcc accaccgtgc cgtccatcgt
    16441 cgcggtaatg cgcgtgtagc ccaaatccga ctcggcggta ttgatggaaa ttttgctctg
    16501 tctgattaaa gccttcaact cggcaacatt ggctttggcg gcggcaagcg catcctgcgc
    16561 gctttccaaa tcttctttag aggtcgcatc atccttccac aacgccgtct gacgcttata
    16621 tttcttctcc gcgctgccca atgcaatctg tgccgacacc agcttcgcct gatacgtttc
    16681 caatttggat ttttccatat tgagcgtgtt ggtctgcgtg gtcgaattga tttccgcaat
    16741 caaatctccc tttttgacct gttgcccgag tttgacataa agcttcttaa tctgccccga
    16801 agcctgcgcg ccgaccgata ccaggttgga cggcgaaatc tcgcccgtcg cggaaaccgt
    16861 ccggctgatg ccgccgcgcc tgaccgtttc cgtaatataa gaagcctgcg gctcgggctt
    16921 cagataatgc catccgcccc aaaccgctgc cgccgcgact gccgcaacag ccgaccattt
    16981 catcattttc gccatatttt caattctctg tatgcgtcaa tcccataaac cggaagcgga
    17041 ttctaccgca agtccctgcc ccatccaagg cggcaaaccg ccgacaataa aaataaccat
    17101 ttgttttaaa tggcttatac aatctgcttt cccgaaaatt tcaatccgtt tacatattcc
    17161 gccatttccg atacggcgtt ataatgccgc ccacaaacaa ccaaatggaa acacattgct
    17221 atgaaaaacg ttcaaaaagg ctttacgctg ctcgagctga tgattgccgt cgccatcctc
    17281 ggcatcctga cgctcatcgc ctacccttcc tataaaacct acatccggcg cgcccgcctg
    17341 tcggaggtca aatccaccct gctggtgaac gcgcaaaact tggagcgtta ctaccgccaa
    17401 aaagggacgt ttcaaaccta cgaaaaaaac aaactgaaac aaaacgaata tttcaagatt
    17461 accttaatca aaaccagccc cgaccacttc acccttaagg ccgaccccaa tccgatgacc
    17521 aacgaaggcg aaacctgcac cgttacgctc aacgacggcg gtaccattgc cgcttccggt
    17581 acaaaccaat cctgccccgg cttcgactga ttggctgttg cagtcggcat tgtgcctaaa
    17641 ccatctataa aaatgccgtc tgaacgctgt tcagacggca tttttgacat cccgatacgc
    17701 ctttcaaacg gcttccgcca tttccaacgc ctgcttgatg tctacggcga cgacgcgcga
    17761 cacgcctttt tcctgcatgg ttacgccgac cagctgctcc gccatttcca tggtcaggcg
    17821 gttgtgggag atgtagagga actgggtttg caccgacatt tctttgacca gcttgcagaa
    17881 acgcgaggtg ttggcatcgt ccagcggggc atcgacttcg tccagaaggc agaagggggc
    17941 ggggttgagg ctgaacaggg cgaacacgag gctcatggcg gtgagggctt tttcgccgcc
    18001 ggagaggagg tggatggtgc tgtttttctt gccgtgcggg cgcgccatga tggacacgcc
    18061 ggcggtcagg aggtcgtcgc ctatcatttt gagggtggct tcgccgccgc cgaacagagt
    18121 cgggaagaag gtttggactt tgccgttgac ggcgtcgaaa gtttctttga aacgcgcttt
    18181 ggttttgtcg tcgatttggg cgatggcttc ttccaaaagc gcgatggcgg cctgcacgtc
    18241 ttcgctctgg ctgcggtagt agccgtcgcg ttcgcgcgct tcttcgagtt cttgcagggc
    18301 ggcgaggttg acggcgccga gggcttcgat ttgccgggta aggctgccga tgctgctgtt
    18361 caatactttc gccgattctt tcgccaacgc ttcgagcacg tccagatcgg cagcgcgttc
    18421 ggtcaggttt tgatggtagc gtttggcgtt gatcagggcc tcctgctgct gcaacagggc
    18481 ggtttgggtg gcggcctgaa gctgcggcag cttggtttgc agggtttgta cgcgggcgta
    18541 ttgctccctg ccctgttcct gaatctgcgc gagtttttcc tgcacggcga tgtattcttc
    18601 gtccagcgtg tgtacggttt cggtcaactc gtcgagcttg atgtgctgct cgtcgttttg
    18661 gaactcggtt tcataggcga gggcaagctc ttgccggcgt tcctgccagt ccagggtttg
    18721 ctgttcgagc cgggcgattt gctgccggta gttttgtttt tgctggttga gtttgtggac
    18781 ggcgacttcg gcaagcccgt attggcggtt ggcttccaac agggcaagct gcgcctgttt
    18841 cagacggcct tgctgctctt ggcggctgtg cgcggtggtt tgctgctggt gttcgagttc
    18901 ggcggcggct tcctgcaaag tagcgatgtc gtctgaaagc ccgtcggacg tgtgttgcaa
    18961 cacggtctgc tcttccgcca actgtgccag ttcgcgctcg atgtgttcgc ggcggatttg
    19021 cccttggttg gtgcgcgcca gaagttcggc ggtgcgttgc tgcgcttgac tgtattggcg
    19081 cgtgtgctgc tgttgctgct gcatcagatt tttgtgctgc acttcggacg agcacacggc
    19141 agcctccgtc tgtttgaacg cggcttcggc ggcggaaagt ccgggggcga ggttttccag
    19201 ttcggccgcg atgccgtcga ggcgtgcttt ttgggcaatc aggctttcct gcgcgggctt
    19261 ggcatagagc aggacgctga ccttatcgac ctgatggcct tcgggtgtga gccagatttg
    19321 atgcgtgccc aaatcgtttt gatgcgcgag ggcgtagctc aaatcgggcg cgcacaatac
    19381 gccgtcgagc cagtggtgca atgccgtctg aaacggcggc tgcgcttgga tttggttcag
    19441 caatgcctgt acgggcaggg attttttgat gccgccggag aggccgtctg aaagccatgc
    19501 cgcctgcccc tgcggcaaag gtgcgggcgg tacgaaacta ttcggcacgc tacgggcgtg
    19561 caggcgttcg gcaaggatga cggacagggc gtgctgccac tcggcgggcg cggtgatgtg
    19621 ttgccacagt tgcggcgcgg cggcgtggtc ggttgcctgc caaaagtcgg cggcttcctg
    19681 ctgttgcgac aggatttgcg acaacgcctg ctgctgcgcc tgcaaggtaa tgtgttgctg
    19741 cttcaggctt tggaagcggt ttgaggccgt ctgaaacgcc tcgcgggcgg cgtgcaaggc
    19801 ttcttcggcg gcaatgattt gttcttcgta atgctcttgc cggctttgca acaaggcggc
    19861 ggcttcctga gcggcggcgg tttcggcctc gtcgggcagg ttcagggctt ggttttcctg
    19921 cttcagacga cctttgcgct cttcgtgctt ggcaacggtt tgttcggcat gggcaagctg
    19981 ctgctgcttc aacgccagtt cgcggcggat gcggtttgcc tcgtcctgct gggtttggaa
    20041 ggcagcgttg agcgtggctt ggacttcttc caattcgggc agacgctctt cgtgttcggc
    20101 aacctgcatc gcccattccg ccaattcggt ttgcttttct tcggcctgca actcgttttc
    20161 ttcaagctgc acgcggattt gctgctgctc ctgccggatg cgttgcaact gcgcctgcgc
    20221 cgcctgcttg tcgcgttcga tgcgttggtg caggttttgc cggtggcgga tttgttcttc
    20281 caaacgggca atctgctcgc gcaacacgcc gcgtttgttg ctcaattcgt gtactgcctg
    20341 ctgctgcgac tgttcggcag tctgcaaggc gtgtacttcg ttatttaacg cctgaacctg
    20401 tgcggcggtt tcatcctgct gcgcctgcaa agattgatgc tgcgcggtcg ccttgtcggc
    20461 ggcggcaagc gattgctgcc attgggcgta atcgagcaaa tcctgttgtt gattcaactg
    20521 cgcggtcagg gatttgtagc gttcggcggt ttccgcctgt ttttccagct tttccacctg
    20581 acgcgccaac tcgttctgca aatcgcccaa acgctgcaaa tgctcgcgcg tgtctttcag
    20641 acggccttcc gtctccttgc ggcgttcctt atatttggac acgcccgccg cctcctcgat
    20701 ataggcgcgc aactcctccg gccgcgcttc gatgatgcgc gaaatcatcc cctgctcaat
    20761 gacggcataa ccgcgcgcgc ccacgcccgt acccaagaac aaatcggtaa tatcgcggcg
    20821 gcgcacggtc tgattgttga tgaaataagt cgattccccc tgacgcgtca gctgccgctt
    20881 gatgctcacc tcggcatact gcccccacgc gccctgcaaa ctgtggtcgc tgttgtcaaa
    20941 taccagctcc accgaagccc tcggcgcagg acggcgcgtc gccgcaccgt taaaaatcac
    21001 gtcctgcata ctctcgccgc gaagctgctt cgccgaagcc tcgcccaaca cccagcgcac
    21061 cgcgtcaatc acattcgact tgccgcagcc gttcggcccg ataaccgcga caagctgccc
    21121 cggaacatga atcgtggtcg ggtcggtaaa agatttgaag ccggagagtt tgatgtgggt
    21181 caggcgcata atggtcggaa aaaataaaaa agaagcgtat tttaacggaa atcccgccgc
    21241 accaccccat atcttgccgg caaaacctta ccgcatcccg ccccggtgcg gcaggatttt
    21301 gcacatcccg gccatccgta ttaaaatctt tgcgtttccc acactttcct gacaaatata
    21361 cggaggagga ttccatgaaa acaaaaacag
//
LOCUS       DAXFHF010000036        21274 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXFHF010000036 DAXFHF010000000
VERSION     DAXFHF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.036"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            211..1053
                     /locus_tag="ACFIRM_001220"
     CDS             211..1053
                     /locus_tag="ACFIRM_001220"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC3028852.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1083..1547
                     /locus_tag="ACFIRM_001221"
     CDS             1083..1547
                     /locus_tag="ACFIRM_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC3028853.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGLRD
                     IPSAELADMVLKELLEHNTEAAVRFAALHKHFDNPADFASWLAQAVKTDRKA"
     gene            1580..2689
                     /gene="ribD"
                     /locus_tag="ACFIRM_001222"
     CDS             1580..2689
                     /gene="ribD"
                     /locus_tag="ACFIRM_001222"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC3028854.1"
                     /translation="MFSDTDISMMENALDLADLGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2884..4302
                     /locus_tag="ACFIRM_001223"
     CDS             2884..4302
                     /locus_tag="ACFIRM_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC3028855.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLGLAVPSGGTRGAAVACAASFWL
                     FFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4299..5365
                     /locus_tag="ACFIRM_001224"
                     /pseudo
     CDS             4299..5365
                     /locus_tag="ACFIRM_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            5379..6545
                     /locus_tag="ACFIRM_001225"
     CDS             5379..6545
                     /locus_tag="ACFIRM_001225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3028856.1"
                     /translation="MNITIAAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPKDAEAASQGRLKVILLEESGYSKNVSLGCVTSHHRFVKHFEKWLENCRPGE
                     QDVIYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKEANPNVPGEVVYIGADFAAIAPPPRFRSKTVRF
                     FYLGTLSYNYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGYIP
                     YAEMMSVAKGCDIAVNAIHSYAMQSITNKLSDYMALQKPILNSQVNDEVAEVLTLLPH
                     ANYRSGDVDGFVRAAKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLADE"
     gene            6538..7779
                     /locus_tag="ACFIRM_001226"
     CDS             6538..7779
                     /locus_tag="ACFIRM_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC3028857.1"
                     /translation="MNKFFKRLIDIVASASGLIALSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAEGETTMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSATIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            7910..9085
                     /locus_tag="ACFIRM_001227"
     CDS             7910..9085
                     /locus_tag="ACFIRM_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC3028858.1"
                     /translation="MLNTALSPWPSFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            9133..11043
                     /locus_tag="ACFIRM_001228"
     CDS             9133..11043
                     /locus_tag="ACFIRM_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC3028859.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVRITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            11099..12391
                     /locus_tag="ACFIRM_001229"
     CDS             11099..12391
                     /locus_tag="ACFIRM_001229"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC3028860.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(12443..13279)
                     /locus_tag="ACFIRM_001230"
     CDS             complement(12443..13279)
                     /locus_tag="ACFIRM_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC3028861.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(13574..13777)
                     /locus_tag="ACFIRM_001231"
     CDS             complement(13574..13777)
                     /locus_tag="ACFIRM_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC3028862.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(13755..13988)
                     /locus_tag="ACFIRM_001232"
     CDS             complement(13755..13988)
                     /locus_tag="ACFIRM_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3028863.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(14000..14236)
                     /locus_tag="ACFIRM_001233"
     CDS             complement(14000..14236)
                     /locus_tag="ACFIRM_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028864.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            14521..17955
                     /gene="dnaE"
                     /locus_tag="ACFIRM_001234"
     CDS             14521..17955
                     /gene="dnaE"
                     /locus_tag="ACFIRM_001234"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC3028865.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            17997..18251
                     /locus_tag="ACFIRM_001235"
                     /pseudo
     CDS             17997..18251
                     /locus_tag="ACFIRM_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18607..19538)
                     /locus_tag="ACFIRM_001236"
                     /pseudo
     CDS             complement(18607..19538)
                     /locus_tag="ACFIRM_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(19608..20168)
                     /locus_tag="ACFIRM_001237"
                     /pseudo
     CDS             complement(19608..20168)
                     /locus_tag="ACFIRM_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(20114..20329)
                     /locus_tag="ACFIRM_001238"
     CDS             complement(20114..20329)
                     /locus_tag="ACFIRM_001238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028866.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            20489..21148
                     /locus_tag="ACFIRM_001239"
     CDS             20489..21148
                     /locus_tag="ACFIRM_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC3028867.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT         4788 a         6540 c         5559 g         4387 t
ORIGIN      
        1 gggaatgacg gctcaaaagt tacggaacga aaaacaaccg aaaccggaca agtcggattc
       61 ccgcctgcgc gggaatgacg gaatcttaag tttccgtctt tgttttctgt tttcgcggga
      121 ataatgggga acaagccgta tttcagacgg cattttcagt tcggggtata atccgaatac
      181 ttgcgaccat ctgaatcatt tggacaaact atgtgtcaac tgctgggcat gaactgcaat
      241 acgccgaccg atattatgtt ttcctttgaa ggcttccgcc gcaggggcgg cattaccgac
      301 catcacgccg acggtttcgg catcggcttt ttcgaaggca aaggcgtgcg cctgttccat
      361 gacgacaagc cgagtgcaaa ttcccccgtc gccgacctcg tgcgcgccta ccagattaaa
      421 tcggaaaacg tcgtcgccca catccgcaaa gcctcgcaag gtcaaacctc gctggcgaac
      481 acccatccct ttatgcgcga aatgtggggc ggctactggc tgtttgccca caacgggcat
      541 ttggtcgatt ttttccccga acagggagaa tttttccacc ccgtcggcac aaccgattcc
      601 gaacgcgcgt tctgccacat cctcaaccgc ctgcgcaccc gctttgccgc ccgtcccgac
      661 gacgacacgc tgtttgacgc gattgcgggg ctgacgcacg aaatccgcaa gttcgggctg
      721 tttaacttca tgctttcaga cggcatttcc ctgtttgccc acgccagcac gctgctgcac
      781 tacatcgtcc gccaagcccc gttcggcaaa gcgcgcctgc tcgacgacga tgtaatggtc
      841 gattttgccg aagtaaccac gccggacgac cgcgtcgccg ttatctccac cctgccgctg
      901 actcgcgatg aatcgtggtc ccaacttgcc gtgaacgaac tggtcatgtt ccgcgaaggc
      961 aacatcgtcc ggcacgaccg tcccgaaaat cccgtctata tgagtgccga agaaggcctg
     1021 gaaatcgccc gcgccgccgg cgtatcggtt tgacgttcag acggcatcag tgaggaaata
     1081 ccatgaaatg cccgttttgc agccacccag acacccgagt tgccgattcc cgcctgatgg
     1141 aagagcgcaa taccgtccgc cgccgccgcc actgccccgg ctgcggcaaa cgcttcggca
     1201 cactcgaaac cgccgaactc aaaatgcccg ccgtcatcgg tccggacaag aaacgttcgc
     1261 ccttcaatgc acaacgcctc cgcaacgacc tgaccgccgc cgcccgaaaa tccgccctga
     1321 cacccgaaca gattgacgaa accgtccgcc tgacggaaca caggctctac acttcgggtc
     1381 ttcgcgacat cccctctgcc gaacttgccg acatggtgct aaaagagctg cttgagcaca
     1441 acacggaagc cgccgtccgt ttcgccgcac tgcacaaaca cttcgacaat ccggcagact
     1501 ttgcctcgtg gctggcgcaa gccgtcaaaa cagaccgcaa agcctgattc tccccaaccc
     1561 atactgatac ggtatcccca tgttttcgga cacagatata tccatgatgg aaaacgccct
     1621 cgaccttgcc gatttggggc gtttctccac ctcgcccaac ccgcgcgtcg gctgcgttat
     1681 cgcacacggc agccaaattg tcggacaggg cttccacgtc aaagcgggcg aaccccatgc
     1741 cgaagtccac gccctgcgtc aggcggggga aatggcaaaa ggcgcgaccg cctttgttac
     1801 cctcgaaccg tgcagccatt acggacgcac accgccctgc gccgaagccc tgctccgttc
     1861 cggcgtaaca cgcgtcgtcg ccgccatgcg cgaccccaac ccgccggttg caggcaaagg
     1921 gcttgtcctg ctcaaagcag cgggcatcaa gacggaatgc ggactacttg aaaacaaagc
     1981 acgcgaactc aaccggggat ttttatcgcg catcgaacgc cgccgcccct ttgtccgcct
     2041 caaatgcgcc gtttcgctgg acggcaaaac cgccctttca gacggcagca gcttttggat
     2101 taccggcgag gaagcgcgtg ccgacgtaca ggttttgcgt gccgaaagct gcgcggtgct
     2161 gaccggcatc ggcacggtgt tggcagacaa tccccggctc aacgtccgcg cttttccaac
     2221 tttgcgccaa cccgcacgca tcgttttaga cagccgcctg cgcctgcccc cgaacagcca
     2281 tttggttacc gacggacaat ctccgaccta catcgccacc ttggaacgcg atgaagacaa
     2341 actccgcccc tatcgggaac acgcacacat ccgcatcctg atgccgtctg aaacggcaga
     2401 cggcaaaatc gacctgcacc atctgatgcg cctccttgcc gacgaaggtt tcggcgaaat
     2461 catggtcgaa gcaggctccg aactcacatc cgcatttttg gcggaaaatt tggcggacga
     2521 aatcgtgctg taccgttcgc ccaaaatcct cggcggcggc aaagacctgt tttccctgcc
     2581 cgaaaaccgt gccgcccttt ccgcaccgcc cttgtggaca cccgtttcaa gcgaaatcct
     2641 cggacacgac atcaaaaccg tgttccggaa aaacggcaac gccttttaaa gggtttgccg
     2701 ccgtttcact atataataac gccgataaaa aacggcccgt tcagaccgcc gcccgcccgg
     2761 aaaagcgcaa cccggactgc cgcaccccgc cggcagccgc gacggcctga aagccgtcaa
     2821 attccgatca agaaaggctt cagacggcac aggcagcatc ccgccgccga ccggacatca
     2881 aaaatggaca caaaagaaat cctcggctac gcggcaggct cgatcggcag cgcggtttta
     2941 gccgtcatca tcctgccgct gctgtcgtgg tatttccccg ccgacgacat cgggcgcatc
     3001 gtgctgatgc agacggcggc gggactgacg gtgtcggtat tgtgcctcgg gctggatcag
     3061 gcatacgtcc gcgaatacta tgccgccgcc gacaaagaca ctttgttcaa aaccctgttc
     3121 ctgccgccgc tgctgttttc cgccgcgata gccgccctgc tgctttcccg cccgtccctg
     3181 ccgtctgaaa tcctgttttc gctcgacgat gccgccgccg gcatcgggct ggtgctgttt
     3241 gaactgagct tcctgcccat ccgctttctc ttactggttt tgcgtatgga agggcgcgcc
     3301 cttgcctttt cgtccgcgca actcgtgccc aaactcgcca ttctgctgct gttgccgctg
     3361 acggtcgggc tgctgcactt tccggcgaac accgccgtcc tgaccgccgt ttacgcgctg
     3421 gcaaaccttg ccgccgccgc ctttttgctg tttcaaaacc gatgccgtct gaaggccgtc
     3481 cggcgcgcgc cgttttcgcc cgccgtcctg caccgggggc tgcgctacgg cataccgctc
     3541 gcactgagca gccttgccta ttgggggctg gcatccgccg accgtttgtt cctgaaaaaa
     3601 tatgcgggcc tggaacagct cggcgtttat tcgatgggta tttcgttcgg cggggcggca
     3661 ttattgctcc aaagcatctt ttcaacggtc tggacaccgt atattttccg tgcaatcgaa
     3721 gaaaacgcca cgcccgcccg gctctcggca acggcagaat ccgccgccgc cctgcttgcc
     3781 tccgccctct gcctgaccgg aattttctcg cccctcgcct ccctcctact gccggaaaac
     3841 tacgccgccg tccggtttac cgtcgtatcg tgcatgttgc cgccgctgtt ttacacgctg
     3901 accgaaatca gcggcatcgg tttgaacgtc gtccgcaaaa cgcgtccgat cgcgcttgcc
     3961 accttgggcg cgctggcggc aaacctgctg ctggggcttg ccgtaccgtc cggcggcacg
     4021 cgcggcgcgg cggttgcctg tgccgcttca ttctggctgt tttttgtttt caagaccgaa
     4081 agctcctgcc gcctgtggca gccgctcaaa cgcctgccgc tttatatgca cacattgttc
     4141 tgcctggcct cctcggcggc ttacacctgc ttcggcacgc cggcaaacta ccccctgttt
     4201 gccggcgtat gggcggcata tctggcaggc tgcatcctgc gccaccggaa agatttgcac
     4261 aaactgtttc attatttgaa aaaacaaggt ttcccattat gaaaatcatc ctgaccactt
     4321 ctatgtcggg cctgggcggc acagaaaccg ccaccgtccg cctcgggcgg ctgctcaaac
     4381 ggcacgggca cgacatcatc ctcgcctcct cggacggccc ttttgtcgga gaggcgcaag
     4441 catcgggcat ccgttggcag ccggttgatt tttaccgggg cgggcttgcc ggctacctta
     4501 aaagcacctt tgcctacgcc cggatgctgc gccgcgaaca acccgacatc atcgactgcc
     4561 aaatggcgcg cgtcgtcccg gcctgcgccc ttgccgccaa aatcgtttca ccgaaaacca
     4621 aaatcatctg ccactcgcac ggtttggacg cggcaaccta ccccaaaacc gccaaactct
     4681 tcgacaagct gggcgcgtac atcatcggca actgcaaaca cgaacgcgaa aagctcatcc
     4741 gccacggctt ccccgccggc cggattgcct acgcctacaa cacccccccc cggaatttca
     4801 tttccggaaa accgaaaaag aatgcgccgt actcggcaca ctttcccgtt tggacaccgt
     4861 ccgcgccgtg catctgatgt tggatatttt gaagaaaatg gttggccgca atatacccgt
     4921 acgcctgaac atggcgggca taggagaaga aatggacaac ctcaaagccc aagccaaacg
     4981 tttgggtatt gaagacaaag tgaccttcct cggcggcgtc cgcgatttga ccggctactt
     5041 caaagaagtc gatattttag tgaacacgcc gcattgcgta ggtgaccacg gcgcaggtgt
     5101 cggcaacaat attttggaag ccggccttta cgacacgcct gtcgtgacct acaacatggc
     5161 gggcatttcc gaaatggtca ttaccggcca aaccggctac tgcattcctt tcggcgatga
     5221 cgaagcgttt atcgaagccg tcgatacact catcaagcat ccagagttgc gcagccaaat
     5281 gggcaaagcc ctgcacaaac atgtcgaaac cttatgctcc gacgacgaaa tctaccgcac
     5341 caccatggct gcgtacgaaa tgtaaggccg tctgaaacat gaacattacc atagccgccc
     5401 cctactgctc gctgccgtcc gaaccgcatt tcaaccgctt ttggtatctg gcagaactat
     5461 tgtcgcaatc gcacgacgtg ttgctgatta ccagcaactt caagcactac gacaaatctt
     5521 tcagacggcc caaagatgcc gaggccgcct cacaaggccg tctgaaagtc atactgttgg
     5581 aagaaagcgg atatagcaaa aacgtatctt tgggatgcgt gaccagccat caccgcttcg
     5641 tcaaacattt tgaaaaatgg ttggaaaact gccgtccggg cgaacaagac gtcatctatt
     5701 ccgcctatcc gctgattgcc accaacctgc tgttgggcaa acacaaagcg cgtttgggct
     5761 acaaactgat tgtcgatgtg caggacgtat ggccggagtc tttctcctcg gtcgtaccgt
     5821 ttttgaaaaa agtaccgcac aagctgctgc cctttgcttc acgcgccaac cgggcctacc
     5881 gctacgccga cgcgctggtt gccgtatcgc agacttatct cgaccgcgcc aaagaagcca
     5941 atccgaacgt acccggcgaa gtcgtctata tcggtgcgga ttttgccgcc atcgcccccc
     6001 ccccgcgatt ccgctccaaa accgtccgct ttttctactt gggcacgctc agttacaact
     6061 atgacgtgga aaccgtgtgc aaaggcgttc gcaagctgtt ggacgacggg gaaaacgtcg
     6121 aattgcacat tatgggcggc ggccccgatt tggacaggct caaacaatat gcctgcgacg
     6181 gcatcaagtt ttacggctac attccttacg ccgaaatgat gtcggtcgcc aagggctgcg
     6241 acattgccgt caacgccatc cactcttacg ccatgcagtc gattaccaac aaactctccg
     6301 actatatggc tttgcaaaaa ccgattctga acagccaggt caacgacgaa gttgccgaag
     6361 tccttaccct gctgccgcat gcgaactacc gttccggcga tgtggacggt ttcgttcgag
     6421 ccgccaaaga tattttgaag cgcaaaaacg atcctgttca gtccgaagaa atcgtccgcc
     6481 gtttcaagcg cgacgttgcc tatcaaaaaa tcgtcaacct gattgaaaga ttagctgatg
     6541 aataaatttt tcaaacgcct catcgacatt gtcgcctccg catcggggct gattgccctg
     6601 tcgcccgtgt ttttggtttt aatatacctc atccgcaaaa acttaggttc gcccgtcttc
     6661 ttcattcggg aacgccccgg aaaggacgga aaacctttta aaatggtcaa attccgttcc
     6721 atgcgcgacg cgcttgattc agacggcatt ccgctgcccg acagcgagcg gctcacgccc
     6781 ttcggcaaaa aactgcgcac cgccagtttg gacgaactgc ctgaactgtg gaacgtcctc
     6841 aaaggcgaga tgagcctggt cggcccccgc ccgcttttga tgcagtatct gccgctttac
     6901 aacaaatttc aaaaccgccg ccacgaaatg aaaccgggca ttaccggctg ggcgcaggtc
     6961 aacgggcgca acgcgctttc gtgggacgaa aagttctcct gcgatgtttg gtacaccgac
     7021 aatttcagct tttggctgga tatgaaaatc ctgtttctga cagtcaaaaa agtcttgatt
     7081 aaagaaggca tttcggcgga aggggaaacc accatgcccc ctttcgcggg gaatcgcaaa
     7141 ctcgccgtta tcggcgcggg cggacacggc aaagtcgttg ccgagcttgc cgccgcactc
     7201 ggcacatacg gcgaaatcgt ttttctggac gaccgcaccc aaggcagcgt caacggcttc
     7261 cccgtcatcg gcacgacgct gctgcttgaa aacagtttat cgcccgaaca attcgacatc
     7321 accgtcgccg tcggcaacaa ccgcatccgc cgccaaatca ccgaaaaagc cgccgcgctc
     7381 ggcttcaaac tgcccgttct gattcatccc gacgcgaccg tctcgccttc tgcaacaatc
     7441 ggacaaggca gcgtcgtaat ggcgaaagcc gtcgtacagg ccggcagcgt attgaaagac
     7501 ggcgtgattg tgaacactgc cgccaccgtc gatcacgact gcctgcttga cgctttcgtc
     7561 cacatcagcc cgggcgcgca cctgtcaggc aacacgcgta tcggcgaaga aagccggata
     7621 ggcacgggcg cgtgcagccg ccagcagaca accgtcggca gcggggttac cgccggtgca
     7681 ggggcggtta tcgtatgcga catcccggac ggcatgaccg tcgcgggcaa cccggcaaag
     7741 ccccttacgg gcaaaaaccc caagaccggg acggcataag cgattaaaat aaacttccgt
     7801 acaaaccgat tttgacaaca cctgcggcgc gcgcccgatt cttcggaaca cgcccccttc
     7861 agacggcata gggtcggaaa tgccgtccga acccgacgga ccaaacacca tgctgaacac
     7921 cgccctttcc ccatggccga gctttaccca agaagaagcc gatgccgttt ccaaagtcct
     7981 gctgtccaac aaagtcaact actggacggg cagcgaatgc cgcgaatttg aaaaagaatt
     8041 tgccgccttt gccggcacgc ggtacgccgt cgccctttcc aacggcacgc ttgcgctcga
     8101 tgccgcactc aaagcaatcg gcataggcgc gggcgacgat gtgattgtta cctcgcgcac
     8161 cttcctcgcc tccgcctcct gcattgtgaa cgcgggcgca aaccccgtgt ttgccgacgt
     8221 ggatttgaac agccaaaaca tcagcgcgga aaccgtcaaa gccgtgctga ccccgaatac
     8281 caaagccgtc attgtcgtcc accttgccgg tatgcctgcc gaaatggacg gcatcatggc
     8341 tttggcaaaa gaacatgatt tgtgggtgat tgaagactgc gcccaagcgc acggcgcgac
     8401 ctacaaaggc aaatccgtcg gctctatcgg gcacgtcggc gcgtggtcgt tctgtcaaga
     8461 caaaatcatc accaccggcg gcgagggcgg tatggttacg accaacgaca aaaccctgtg
     8521 ggaaaaaatg tgggcgtaca aagaccacgg caaaagctac gatgccgtgt accaccgcga
     8581 acacgcgccc ggtttccgct ggctgcacga aagtttcggc accaactggc gcatgatgga
     8641 aatgcaggct gtcatcggcc gcatccagct caaacacctg cccgaatgga cggcgcgccg
     8701 ccaagaaaac gccgccaagc tggcagaaag tttgcgcaaa ttcaaaagca tccgcctgat
     8761 tgaagtcgcc ggctacatcg gacacgcgca atacaagttc tacgccttcg tcaaacccga
     8821 acacctgaaa gacgactgga cgcgcgaccg catcgtcagc gaactgaacg cgcgcaacgt
     8881 cccctgctat caaggcggct gctccgaagt gtatttggaa aaagccttcg acaacacgcc
     8941 gtggcggccc aaagagcggc tgaaaaacgc cgtcgaactg ggcggcaccg ccctgacgtt
     9001 cttggtgcac ccgacgctga ccgacgacga aatcgcgttc tgcaaaaaac acatcgaagc
     9061 cgtcctgacc gaagccgcac gataaccctt cagacggcat atgccgcctg aaagcacata
     9121 ccgccccacg atatgaatct ggaaaccctg atcgccctgc cgcgcaacat caagaaaatc
     9181 tgtttcctca tacacgattt tctgatgatt ttcattgcct tttggttcac ccaaagcctg
     9241 aaggccgact actcggacga atggttcgat tttgccaact ggcagtcttt tttgctgact
     9301 gccttgctga ccatcacatt atttatccga atggggcttt accgcgccgt tacacgcttt
     9361 gtcagtttcc acgtcctgac caccgccttt gccggcagcc tcgcctccgc cgtattgttc
     9421 ttcctcaaca cattgatttt tgaagaaagg ctgcgcctcg ccctgccgat tgtctatttc
     9481 ttactgctgt ttgtttccgt gaccggctcg cgtatggttt tgcgcggact cctgtccgac
     9541 caccagaaaa aaacacatga ccccgtcatc atttacggcg cggggcgttc gggtcggcag
     9601 ctccttgaag ccgtcaaaca gatgcgcgaa tattccgccg ccgcctttgt ggacgacaac
     9661 cccaagcttt ggcacaccgt catctacgac cttgccgttt accagcccga tgccatcgcc
     9721 ttcctcatcg aacgctacgg cgtggaaaaa atcctgctcg ccattcccag cgcgactcag
     9781 gaacaacgcc gccgaatcat cagcaaactg aaagcctatc cgtgcgaagt gttgaccatt
     9841 cccggaatga aagacctgat ggacgggaaa atcagcatcg gcacgctcaa aaaaatctct
     9901 gtgtccgacc tgctcgggcg cgattccgtc gcacccgacg accgcctcat gaatgccgac
     9961 accgaaggca aaaccgtcat ggtaaccggc gcgggcggct ccatcggttc ggaactctgc
    10021 cgccagatta tccgccgccg ccccgaaagg ctgctgctgt tcgagctgtc cgaattcgcc
    10081 ctgtacaccg ttgaaaaaga attgtgcgaa tactgcgccc gaaacggcat cgccgccgaa
    10141 atccttccct ttttgggttc ggtgcaaaac cgcacgctgc tcacgcacat tatgaccgcc
    10201 ttttccgttg cgaccgtcta tcacgccgcc gcctacaaac acgtccctat ggtcgagttc
    10261 aacaccgtcg agggcatacg caacaacatc ttcggcacac tcgaatgcgc gcttgccgcc
    10321 acggcttcgg gcgtggaaac cttcgtcctc atctccaccg acaaagccgt ccgccccgcc
    10381 aacacgatgg gtgcgggcaa acgcatggcg gaactctgcc ttcaggcact cgccgccgaa
    10441 cccggacaaa aaacccgctt cagcatggta cgtttcggca atgttttagg ctcgtccggc
    10501 tccgtcgtcc cgctgtttga aaaacagatt gccgaaggcg gccccatcac cctgacccac
    10561 cccgacatca cgcgctattt catgaccata cccgaagcgg cgcaactggt tatccaagcc
    10621 ggcgcgatgg gcagaggcgg cgacgtgttc gtcctcgata tgggcgaatc cgtcagaatc
    10681 accgaccttg cccgccaaat gattacctta agcggactca aacccaaaac gcccgaacag
    10741 cccgacggcg acatcgaaat cctcattacc gggctgcgcc ccggcgaaaa actctacgaa
    10801 gagttgctca tcggcgacaa cgtccgcaaa accggccatc cgcgcatcat gaccgccgac
    10861 gagaccatgc tgccgtggca cgaactctcc gccctgctcg accgcatccg caccgcctgc
    10921 gaccgttacg accagcaggc aatccgcgac ctgctcgtca acgccccgac cggctttacc
    10981 ccgaccggcg gcatctgcga cctgctttgg gtatgcgaaa cacacagaaa aaatgccgtc
    11041 tgaaccttca gacggcatac cgtacaaacc aatctgcctt acacacactg agtttgacat
    11101 gcagttctcg gcattcggcg aaaaattcac gcaacacagc ggcatcctgc aactgatgga
    11161 cgacctcggc gacgcgctca aaagcgacaa gcccgtcaat atgctcggcg gcggcaaccc
    11221 ggcgcgcatt cccgaaatcg atcgggcgtt cgccgacata ttttccaagc tggcggcgga
    11281 acacgccgtc gagaacatcg gcaactactc caatccccaa ggcgatgccg cgctgattga
    11341 cgcgctgacc gccttcccca accgcgaata cggctggaac ctgaccgtcg gcaatatcgc
    11401 gctaaccaac ggttcgcaaa acgcgttttt ctatttattc aacctcttcg gcggcaaatt
    11461 caagctttca gacggcacat ccgcagaaaa agccattttg ttgccgctcg cgcccgaata
    11521 catcggctat gccgacgtgc atgtcgaagg gcggcacttc gtttccgtca aacccaaaat
    11581 cgaaaacgtc gaacacgaag gcgaagcagg cttcttcaaa taccgcgtgg actttgacgc
    11641 actggaaaac ctgcccgaac tcaaagcggg caaaatcggc gcgatttgct gttcgcgccc
    11701 gaccaacccg accggcaacg tgttgaccga cggcgaaatg gcgcgtttgg acgctttggc
    11761 gcgtgaacac ggcattccgc cgattatcga caacgcctac ggaatgccgt tccccaacat
    11821 tattgacagc ggcgtaacgc tgaattggca cgaaaacatc atcctctgct tcagcctttc
    11881 caaagtcggc ctgccgggcg ttcgcaccgg catcatcgtc gccgcgcccg aagccgtcaa
    11941 agccgtcagc agcctgaacg cgattgtgaa ccttgccccc acgcgcttcg gcgcggccat
    12001 cgccgccccg ctgctggaaa gcggcgagat gaaacggctt gccgaccaag tcatccggcc
    12061 gttttaccgc aatcaggcgc aaaccgccgt ttcgctgctc aagcgcgagc tgggcgcgta
    12121 cccgatgaaa atccacaaac ccgaaggcgc gattttcctg tggctctggt ttgaaaacct
    12181 gcccgtttcg tcgcaaaccc tgtacgaaat gctcaaagcc gaaggtacgc tgattattcc
    12241 gggcgaacat ttcttcgtcg gcatcgacac gcaagactac ccgcacgcgg gcgagtgcat
    12301 ccgtatgagt atcgcacagg acgctcaaac gctggaaaaa ggcatcgccg ccatcggcaa
    12361 aaccgtccga aaaccgtacg acaacgttta aaacgcaaaa aatgccgtct gaagaccttt
    12421 cagacggcat tttcatccgc attcaatgtc gggaaaaatg cgcccgcacc ggtttgcagt
    12481 tttccggaag atcgggacac gcgccgagga tgccgccgct gaagtcgtct aaacggtgga
    12541 agtcgctgcc cgcgcttgcc aacaatccga agcggtctgc caaaagcgcg tagttgaggc
    12601 ggtcgttttt gcagcagttg ccgctgtgga cttcgatgcc cgcgccgccg aggtttttaa
    12661 attcttcaaa caggttgcgc ttggcggttg ccgacaaatc ataacgcatc ggatgggcaa
    12721 tcaccgccat gccgcccgcg ccgttgacgg cggagacgca gtctgccagc gtcgcccatt
    12781 cgtggcgtac cgcgcaggat ttgccgtcgc ccaagtattt ggtgaacgcc tgctgcttgt
    12841 ttttgacgtg tccgtttttg atgaggaact cggcgacgtg ggtgcggctg accatttctt
    12901 tgtttgccgc cagcgccagc gcgccgtcgt atgcgccgcc gatgcctttc ttttcgagct
    12961 tggcggcgat ggcttcaaga cgtttcagac ggcctttgcg gacatctgcc aacaggtttt
    13021 gcaggttttc gtcctgctcg tcgaaatcca aaccgacaac gtgtatggta cgcccgcgcc
    13081 acgttaccga aatttccacg ccgttaatca ggcgcaaacc gagcctgtcg gcttcggcac
    13141 gcgcttcgga gatgccgccc gtgtggtcgt ggtcggtcaa cgccagcagc gtgcagccgt
    13201 tttgatgtgc gaggcggacg acttcggcgg gggggagcat accgtcggaa acggtggaat
    13261 ggcagtgcag gtctatcatg gggtgttatg tggtggtgaa tgaaggacgg gatgtttcaa
    13321 tgttgtcgga cagtgtggcg gcggcggtca ttttaaaaaa caggtgtatg gcaaatgcaa
    13381 aggaaaagcc gctatgccgt cattcccgcg caggcgagaa tccagacttt gatttattgg
    13441 aaatgtttaa ggttaaccgc tatttcaaac ttccggattc ctgctttcga gggaatggcg
    13501 atatgaacgt tttcaatttt aatctactat aaaagaccgt ctgaaaacgt ggttttatca
    13561 agcccaaccg ttttcagacg gcatcgtccg gccaacccgc ttccttcagt tttttccgtt
    13621 gcgcatcgta gcgtgccttg tccgcccagt aaatcgtctg aatgcacgcc tcgccgcagt
    13681 cgctgccgca gcattcccac gactcggggc ggacgggttc gtccaaaagc ggctcgccca
    13741 aaagggcttc ggctttatgc ttcagggtcg tatccatcgg cgatttccaa gcgcgcgccg
    13801 tcaaactcga tgacttctcc gccgcgtatt ttggccgttt tgcggatttc ggttccgccg
    13861 ttgcgcaaca ccagcccttc ggcgataaat gcttttgcct gtccgccgct ttcggcaagt
    13921 ccggccaatt tcaagaggtc gcacaaagcg atgtattcgt tgtcttcgag atagacggtg
    13981 gcttccataa tgtccccttt tagaaagagg gcgttattgt agcacctgcc gccgccgcac
    14041 ccaaaattgc cgcaaaaccg gcgatgtgtc cgcactgcct gttccgtaaa aatgccgtct
    14101 gaaaccccgt atgccgccat ccgtccaaag aaatcctgcc caacggcagg ctgcaaatcc
    14161 tgttccccga cgaatccgca ttgacgctga tgcacatcct caaacgcgga ctgcccgata
    14221 caccggcaat cggcatcaaa acgaaatcaa aaacctgttt gaacgtaaaa tctatttaac
    14281 tgcttgccgt ccgtccgaat aaggtatata gttccttata actagtttga tagtccttta
    14341 tatctatcaa tactccttgg gaagcctccg ccacacggca ggaggcattt ttttgccgtc
    14401 gtaaaagctc aaaaaacatt tacaggtcat gccgtctgaa tccgaaacgg cattacctgc
    14461 accgccatct aaagacaacc ctgctacaat acgcctttta ttgtccacgc cgattttgcc
    14521 atgaccgagc cgacctacat ccccctgcgc ctgcataccg aattctccat taccgacggt
    14581 atggtgcgga ttaaaaaact gattgccaaa gcgcaagaat acggtttgcc tgctttgggc
    14641 atcagcgatt tgatgaacga attcggtttg gtgaaattct ataaagcctg ccgcggcgca
    14701 gggattaagc ccgtcggcgc ggcggacgtg tggataggca atccgaatgc gcccgacaag
    14761 ccgttccgcg ccatgctggt tatccgcaac gatgcgggtt atctgcgctt gagcgagctt
    14821 ttgacggagg cttatgtcgg ccaagaccgc aatatccatc atgcggaact caatcccgaa
    14881 tggctggaaa acggcgacaa cagcggcttg atttgtttga gcggcgcgca ttacggcgaa
    14941 gtgggcgtga atctgttgaa cggcaatgaa gacgcggcgc gtgcggcggc gttgaaatat
    15001 gcggcatggt ttcccgatac gttttattta gagctgcaac gcctgcccga acgccccgaa
    15061 tgggaggctt gcgtttccgg cagcgtgaag ctggcggagg aactgggttt gccggtggta
    15121 gcaacgcatc cgacacagtt tatgaaccgc gacgatttca acgcgcacga ggcgcgggtg
    15181 tgcatcgcgg gcggctgggt gctgacggac aagaaacgtc cgcgcgattt cacgccgagc
    15241 cagtttttca ttccgccgga aacgatgctg gagcgtttcg ccgacttgcc cgaagccttg
    15301 gaaaacacgg tggaaatcgc caaacgctgc aacttgcaca tcacattggg caaaaacttc
    15361 ctaccccttt tccccacgcc cgacggttta tcactcgatg actgtctcat caaactttcc
    15421 aacgagggtt tgcaggaacg catggttcag ctttatcccg acgaggcgga acgggcggca
    15481 aaaatgccgg aatatcagga gcggctggac tttgagctga acatcatcat ccaaatgaaa
    15541 ttccccggct atttccttat cgtacaagac tttatcaact gggcgaaaac gcacggctgt
    15601 ccggtcgggc cgggacgcgg ttcgggcgcg ggttcgctgg tggcgtattc gctgaagatt
    15661 accgacctcg acccgctgaa atacgcgctg ctgttcgagc gtttcctaaa ccccgaacgc
    15721 gtttccatgc ccgactttga cgtggacttc tgtcaggcaa accgcggccg cgtgattgaa
    15781 tacgtgcgcg aaaaatacgg cgctcaggcg gtcagccaga ttgtgacttt cggcacgatg
    15841 tcgtccaaag cggtcatccg cgacgtgggg cgtgtgctgg aactgccgtt tatgctgtgc
    15901 gacaaactgt ccaagctgat tccgttggaa gccaacaaac ccctgagttt ggacgacgcg
    15961 atgaaggcgc agccgcagat tcaggaattg cttgaagcgg aagaagcgga cgaactgatt
    16021 acgctggcga aaaaactgga agacctgacg cgcggtttgg gtatgcacgc aggcggcgtg
    16081 ttgattgcgc cgggcaagat ttccgattac agccccgtgt atcaggcgga cgaatccgcc
    16141 tcgcccgtat ccatgtacga caagggcgac gtggaagacg tgggtttggt gaagttcgac
    16201 tttttgggcc tgcgcaacct gaccattatc gaaatggcgc agaacaacat caaaaacacc
    16261 gccggcgata tcgtcgatgt cggcaagatt ccgcttgacg accagactgc ctaccaaatc
    16321 ttccgcgatg cgaacaccac cgccgtcttc cagttcgagt cgaccggcat gaaaaaaatg
    16381 ctgaaaacgg cgcatacgac caagtttgaa gaactcatcg ccttcgtatc gctctaccga
    16441 cccggcccga tggacaacat tcccgacttc gtcgcacgca tgaagggaca ggaattccaa
    16501 tacatccacc cgctgctgga aggcatcctc gcgccgacct acggcattat ggtgtatcag
    16561 gaacaagtga tgcaggcggc gcagattatc ggcggctact cgctcggcgg cgcggacctg
    16621 ctgcgccgcg ccatgggtaa gaaaaagccc gaagagatgg tgaaacaccg cgaaatcttc
    16681 gccgaaggtg cggcaaaaca aggcatttcg cgcgaaaaat ccgacgaaat cttcaactac
    16741 atggaaaaat tcgccggcta cggtttcaac aaatcccacg ccgccgccta cgccctgatt
    16801 tcctaccaga ccgcatggct caaagcccac tatcccgccg aatttatggc ggcgacgatg
    16861 tcgtccgaat tggacaacac cgaccagctc aaacatttct acgacgactg ccgcgccaac
    16921 ggcatcgagt tcctgccgcc cgacattaac gaatccgact accgcttcac gccgtatccg
    16981 aacatgaaaa tccgctacgc actcggcgcg attaaaggca cgggcgaagc cgccgtcgaa
    17041 tccatcatcg ccgcgcggca aagcggcggc aaatttaccg gcctgttgga cttctgcgag
    17101 cgcgtcggca aagaacacat gaaccgccgc accctcgaag ccctgatacg cggcggcgcg
    17161 ttcgacagca tcgaacccaa ccgcgccatg ctcttggcga acatcgacct tgccatggac
    17221 aacgccgacc aaaaagccgc caacgccaat cagggcgggc tgtttgacat gatggaagac
    17281 gccatcgaac cggtgcggct catcgacgca cccatgtgga gcgaatcgga aaaactcgcc
    17341 gaagaaaaaa ccgtcatcgg cttttacctg tccggccacc cgttcggccc gtatgcccaa
    17401 gaagtccgcc aaatcgcccc gaccaaatta ggccgtctga aaccgcaaga cagcgtgcgc
    17461 ctcgccggat tcgttaccgc cgtgcgcacc atgatgggca aacgcggcaa aatcgccttc
    17521 gtcagcctcg aagattcgag cgggcaggct gaaatcatgg tcggcggaca aacgctggaa
    17581 aactgcgccg actgcctcaa agccgaccaa gtgctgatta tcgaatccaa agtcagccgc
    17641 gacgattacg gcggcggcga cgggctgcgc atcatggcaa accaagtcat gaccctgcaa
    17701 acggcgcgcg aacgctacgc ccgcagcctc agcctcgccc ttgccccgca tcacgacatc
    17761 ggcggactgg tacggctgct cgccgcccac caactgcccg acacgccgcg catcccgctt
    17821 caattgtcgt atgccaacga aaaagcgtcg ggcaggcttc aagtgcctcc gaaatggacg
    17881 gttacaccga gttccgcatt gttcggcgaa ctggaaacat tgctcggcag ccggtcggtg
    17941 cgcgtgaatt ggtaaccaaa aatacaaatg ccgtctgaag cccaaaaccg gtttcattcg
    18001 tactttattc gaatgattga ataaaagtaa ctgccaagaa aaacgtattt tttggttatt
    18061 tcgccagcct aaataaatca accggggcga ttgatatccg tgtacatgac acagacagcg
    18121 ccaaagggaa aaacggcatt ttcaaaagta tcggtgtcaa aaccgccctt tcattccaaa
    18181 aataccaaat cgacgaaccg ggcaaagaaa tcagaccgtg ccgcctgaaa aaacgcccgc
    18241 ccgtccgtta aattaatcta tccctgtttc agacggcctg aagcagggat ttttatatca
    18301 agataaaatg agaacgggag cgacaaccac gggcaaagtc ggcgcgaact atcaaaaatt
    18361 gtagcactgc gaaatgagga cgggagcaat aacccttagg tagctcttgt tatagcggat
    18421 taacgaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggaaccgatt
    18481 cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcgg ggcaacgccg
    18541 taccggtttt tgttaatccg ctatattttc cgatgcaaaa caaagcagtc atatatttga
    18601 ttccccctac cgttgccacg cttccctcaa atattcgacg cagtcggtca gcgagtaaaa
    18661 cgggacattg ccgtggtcgg cattcggata ttcccggaaa aatacggcgg tcccgcgcct
    18721 gtccaactct gccgccattt gttcggcctg ccctgccatg tcgcgttctt ccctgcgttt
    18781 gcaaccgctg ctccgctcga gtgcgccgat gttgaggcag gcatcgatgc cgtccagccg
    18841 gttttcagac ggcataaagt cgagtatccg cctgttgtgc caccaaatcg agggggatac
    18901 gagccaatgc cgtctgaaac agcggtggga aagcagggaa tacagtccga acagtgcgcc
    18961 gaacgagtgt ccgaatacgg cggtttcatt acggttaagg gtgtagcggc tttctaaaaa
    19021 ggcggtcagt tcgctgtcga taaagtcggc gaagcggtct gcctgtccga actgccgccg
    19081 ttcgtctgct gtggcgttgt ctccaagcgg cggcgtgtag tcggcggcac gctgcgccag
    19141 gtcgcgcaca ctgcctgtcg tgtagccgat accgacaatc aggcaggggg cgttgcttcg
    19201 ggtaacgggg ttgttcatca gcgactgcat gatgttgtac agcgcgggaa aaaggcttcg
    19261 ccgtcgagga caaagaggac gggatagcct tcagactgca tttcgccgag tgttgccgtc
    19321 tgaatccgat agattcgccc cgtgcaggtg gatttgattt cggtttcaaa ggcttggggc
    19381 agtatggcag gttggaatgt gtcggtcggt atgggtttca tgatgttcgg cttgtgggtc
    19441 agactggcgg caagcggtaa aaccgaaccg gcaaagcggt ctgcctgccg gttcggtctg
    19501 ttttccttcg caatgccata ctccggttgt gagcgcatag ggtatgccgc gcagcttgtt
    19561 gtggtttgat tatgctgcgg ctgccgtttc agacggcata ttggtcttta aaaactgtaa
    19621 cgcaggtttg ccgtcaggct gcgctccgaa ccgggaatgt taaaggtgtt cgtgccgccg
    19681 acgcgggcgt agtaatggcg gttgaagatg ttgtcggcat tgatttgcag cttcagcttg
    19741 ggcgtgaagc ggtatgccgc catcgcatcg aacgtggcat aaccgcctgc atgcatccct
    19801 gcagatgaag taatgccgct catcgcgttc acgccgccgc cgacggtcag cccgggcgta
    19861 acttggtaag tcgtccacag gtttgcgctg tgtttgggca ccagcaggaa gatgccgtcg
    19921 tcgcgtggat tggcggcggt tttgatttgg ctgtgcagat agctgtaacc tgcatggatt
    19981 tgccatttcg gtgtaatcgc accgctgatt tcggtctcaa cgccttccat cacgcgtttg
    20041 cccaatgcgg cgtaacgggt gttgcggttg ttgcggttgt tcgggttcgg cggtgcggcg
    20101 gcgtttttgt ctttcaggcg gcagggggag acgcgggtat tgaggcggtc atccatatag
    20161 ctgcctttaa cgagctgtaa tcgccgtttt tctcggtggc aatcgcgttg tacgaacgct
    20221 gccctttgat atggacggtt tccaaatccg ccgtttgtgc ggcaaaaacc ggagacgaaa
    20281 gtgctgccaa aaccgtggcg gcggtcatat tgatgcggaa aactgacata aactgtccca
    20341 tctgtataaa tgataatggt tctattttaa taaagcacaa cgcggcttgt tcggaaaaac
    20401 atatcgctca gccgacaaat tttgtcgaaa atgcgacacg tctgcgtttt ccgcataaaa
    20461 tctgcttttt tactgcaacc aacctgccat gaccccgccc aaactcatca tcttcgactg
    20521 ggacggcacg cttgccgata cgacccagcc catcatcgac accatgcgcc gcagcttcgc
    20581 cgaatgcggt tttccgccgc ccgaagcgga gcgcgtccgc agcctgatcg gatacagcct
    20641 gcccgaaatc atccgcgccc tgctcgaaat gccttctgaa gctgccgttg ccgacatcgc
    20701 gcgcacttat tccgcgcatt acctcaatcc caacaaccgc aatatgacac tctttcccga
    20761 tgccccgccc tgtctggata agctcaaagc gcaaggcttc cggcttgccg tcgccacggg
    20821 caaagggcgg gcgggtttgg acaacgccat cagccaaacc gccaccggcg gctattggct
    20881 ggccaccgcc tgcgcggggg aatacccctc caaaccctcg cccgaaatgg tattcggact
    20941 ctgcggcgaa ctgggactcg acccgaaaga ggcattggtt gtcggcgata cggcgcacga
    21001 cctgcatatg gcggcaaacg caggcgcggc ggcagtcggc gttgccaccg gcgcacattc
    21061 gcgcgaacag ctccttggcg caccgcatct cgccgtattg gacggtttgt ccgaactgcc
    21121 cggttttctt gcacgacatt acgcctgatt ggtttccgca tccggcacgc ggcaaaaatg
    21181 ccgtctgaag cctgttcaga cggcatgagg cctttgcaaa aaagcccttc cttcggcatc
    21241 cgaaacccaa acacaggttt tcggctgttt tcgc
//
LOCUS       DAXFHF010000037        21082 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXFHF010000037 DAXFHF010000000
VERSION     DAXFHF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21082
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.037"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..1001)
                     /locus_tag="ACFIRM_001240"
     CDS             complement(<1..1001)
                     /locus_tag="ACFIRM_001240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC3028868.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQI"
     gene            1150..2676
                     /gene="ilvA"
                     /locus_tag="ACFIRM_001241"
     CDS             1150..2676
                     /gene="ilvA"
                     /locus_tag="ACFIRM_001241"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC3028869.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGGDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(2732..3808)
                     /locus_tag="ACFIRM_001242"
     CDS             complement(2732..3808)
                     /locus_tag="ACFIRM_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC3028870.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSTEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(3805..4665)
                     /gene="cysW"
                     /locus_tag="ACFIRM_001243"
     CDS             complement(3805..4665)
                     /gene="cysW"
                     /locus_tag="ACFIRM_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC3028871.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWNLYLKSLSDPEAWSAVKLTLITALIVVPVNAVLGVAMAWLLIRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIRIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(4854..5681)
                     /gene="cysT"
                     /locus_tag="ACFIRM_001244"
     CDS             complement(4854..5681)
                     /gene="cysT"
                     /locus_tag="ACFIRM_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC3028872.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAEIGWGGF
                     RNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDLPFA
                     LPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQPVL
                     KELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFIAGS
                     IPMISEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGAKG"
     gene            5862..6194
                     /locus_tag="ACFIRM_001245"
     CDS             5862..6194
                     /locus_tag="ACFIRM_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028873.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            6521..7030
                     /locus_tag="ACFIRM_001246"
     CDS             6521..7030
                     /locus_tag="ACFIRM_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC3028874.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(7262..7843)
                     /locus_tag="ACFIRM_001247"
     CDS             complement(7262..7843)
                     /locus_tag="ACFIRM_001247"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC3028875.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAEWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLALVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAA"
     gene            8007..9413
                     /gene="dnaB"
                     /locus_tag="ACFIRM_001248"
     CDS             8007..9413
                     /gene="dnaB"
                     /locus_tag="ACFIRM_001248"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC3028876.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSITKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            9721..10383
                     /locus_tag="ACFIRM_001249"
     CDS             9721..10383
                     /locus_tag="ACFIRM_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC3028877.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPAQVKKDGTGNNRCDLGKKEQGMLAFGDKNGNK
                     AYDGDVADVFLRSVVLNDTDDSRINYAFNHIAFGSSQPTAGRVVWTFNQNGTFGYLSD
                     QNLKDNSKFVYSDGYIQIVLTDARAVSDADRKFRSAVVLIDSSGRVEVCRKNDTRAVC
                     KH"
     gene            10415..11029
                     /gene="pilV"
                     /locus_tag="ACFIRM_001250"
     CDS             10415..11029
                     /gene="pilV"
                     /locus_tag="ACFIRM_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC3028878.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLINPTIDPDGNKKNYNLYTESYTPTPSGGDFKLNNN
                     NLISKKDLAKAQLDRFGYELKNALPDAVDIHYAVCKDSSGKAPTLSGGTFFPNCDNKA
                     NGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            11026..11967
                     /locus_tag="ACFIRM_001251"
     CDS             11026..11967
                     /locus_tag="ACFIRM_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC3028879.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIGNDVFSNVAQKNAL
                     FSLKRNSTNSTNKLIPITESPNINYRNFFQVSSALIFQYGIDDVNASAATTVVSSCAA
                     ISKPGKQIPTLEDAKKELKIVSQDKEQNGNIARQRHVVNAYAVGKIAGEGGLFRFQLD
                     DKGEWGNPQLLVKKVKRMKVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVL
                     LDSGNDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            11946..12545
                     /locus_tag="ACFIRM_001252"
     CDS             11946..12545
                     /locus_tag="ACFIRM_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC3028880.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYTADSKVTFSENCENGLCTAVNVRT
                     NNNGNEEAFGNIVVKGKPIVEAVKRSCPANSASLCIDNKGMEYRKGTAGVSKMPRYII
                     EYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            12547..13020
                     /locus_tag="ACFIRM_001253"
     CDS             12547..13020
                     /locus_tag="ACFIRM_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC3028881.1"
                     /translation="MEQKGFTLIEVMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPLDDNQTIKSKLEIFVLDYKMNPKIAKKYSVSVRFVDAEKPRA
                     YRLVGIPKTGTGYTLSVWMNSVGDGYKCRDATSAQAYSETLSANTGCEAFSNRKK"
     gene            complement(13090..13398)
                     /locus_tag="ACFIRM_001254"
     CDS             complement(13090..13398)
                     /locus_tag="ACFIRM_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFC3028882.1"
                     /translation="MKDLLSSPSFLLFSCMLAVTCPTRLIGFFALRNRTLSRRAQTVM
                     EAAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(13395..14106)
                     /locus_tag="ACFIRM_001255"
                     /pseudo
     CDS             complement(13395..14106)
                     /locus_tag="ACFIRM_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            14272..14724
                     /gene="dut"
                     /locus_tag="ACFIRM_001256"
     CDS             14272..14724
                     /gene="dut"
                     /locus_tag="ACFIRM_001256"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC3028883.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTLLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            14802..15989
                     /gene="dapC"
                     /locus_tag="ACFIRM_001257"
     CDS             14802..15989
                     /gene="dapC"
                     /locus_tag="ACFIRM_001257"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC3028884.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLDPGSDGMKPVIVSQNPFYQIYEGAAVLGGGEIHFANCPAPSFNPDWCSI
                     SQEVWKRTKLVFVCSPGNPSGSVPDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQHASIAAWNDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            16145..16924
                     /gene="yaaA"
                     /locus_tag="ACFIRM_001258"
     CDS             16145..16924
                     /gene="yaaA"
                     /locus_tag="ACFIRM_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC3028885.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGGIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            17007..17097
                     /locus_tag="ACFIRM_001259"
     tRNA            17007..17097
                     /locus_tag="ACFIRM_001259"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17041..17043,aa:Ser,seq:tga)
     gene            17175..17261
                     /locus_tag="ACFIRM_001260"
     tRNA            17175..17261
                     /locus_tag="ACFIRM_001260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17209..17211,aa:Ser,seq:cga)
     gene            17455..18651
                     /locus_tag="ACFIRM_001261"
     CDS             17455..18651
                     /locus_tag="ACFIRM_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3028886.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGRRSWK
                     LSFMQDGRQQTISLGRYPDFSLAEAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMDINPVAQIGSGVFERVKTKNMTALSPSELPRLIDFLE
                     QRNEFAVYAGRVHIHPVTRFCIYWLLLTMTRIREAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMRWVLDQARGLNVNGRFVFESVNFQGHINKESPHVAMRRSGLDTTAHG
                     LRSLARTYLREVLKVYNDVAEKLLAHSLGTRTQTAYNRSELWEERKDAPERWGNDVLR
                     LADNGK"
     gene            complement(19007..19276)
                     /locus_tag="ACFIRM_001262"
     CDS             complement(19007..19276)
                     /locus_tag="ACFIRM_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028887.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(19471..20154)
                     /locus_tag="ACFIRM_001263"
     CDS             complement(19471..20154)
                     /locus_tag="ACFIRM_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC3028888.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            complement(20468..20701)
                     /locus_tag="ACFIRM_001264"
                     /pseudo
     CDS             complement(20468..20701)
                     /locus_tag="ACFIRM_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5033 a         4915 c         5686 g         5448 t
ORIGIN      
        1 aaaatctgtt ctgccatttt cgcttcctta tgtggcagag tgatgtaggc ttctttgaga
       61 aagcctgcgc ggacggtttt tttgctgcct ccggaaattt ggatttgggc aacggttttg
      121 cctttcggat atattttggg cgtatcgaag gcctgcaatg cccggttcag cagcttgctg
      181 ttgtccgatg cgcgggtttc cgccgattcc gaacctagtg tgatgacaag gatgtgcctg
      241 ccgttgccgg agtatgacac ggcaaggttg tagccgccgc tttctgtgtg cccggctttc
      301 aggccgttta cattgttgtc cctatataaa aggatattgc ggttgttttg ttctatgttt
      361 tcaaacttga acgatttgat ggaaaacagc gggtaatatt ccggaaagtc gcgcatcaat
      421 gcttcagaca gcagggcgag gtctttggcg gtggaaacct gtccttctct acccaagcct
      481 gtcgggtttt tgaatacggt gttcttcatg cccaagcgtc gggcttcttt gttcatttgt
      541 tgcacaaaat tttcaatcga gccgttgccc agccggtcgg caagggttag ggcggcatcg
      601 tttgcgcata gggcaatcat gccttttaag agtttgtcgg tactgaccgt atcgccggga
      661 cgtacaaaca ttctgcttcc ttctgaagcc catgcggatt cgggtatttt taagttttct
      721 tcagattgga tatttcccga tttcatgttt ttgaaaacca aatatgcggt catcagttgg
      781 gttagtgccg ccggttcgac aggggtattg gtgtttttgg cggataaagt ctgcctgctt
      841 tgaaggtcga taacgatgtg tgccgccgtg agggtttcgg gtgtttggag cgtgggggcg
      901 gcgtgtaccg tcggtctgtt gggcgcgggc gatgcagccg ttgcgtgaga aacgcctaag
      961 atgatgggaa gaaggacggg caggatttta tgtgctgtca tgaaatattc taaattgtgt
     1021 gagtgtttca gcctgccgat tatacgctta gggtgtctga tcgggcggat ttttcttgct
     1081 ttcgcgccgt cttgggcgta tggttttggg ttttgcgatt ttaataaacc gattatcccg
     1141 cattgaatta tgaacacgcc ccttccttat tccgattacc tcatccgcat cctgacggcg
     1201 tctgtctatg atgtagcggt cgaaacgcct ttggaaccgg cacgcagcct ttctgtacgt
     1261 ttgaaaaaca acatcctttt gaaacgcgaa gatttgcagc cggttttttc gttcaaaata
     1321 cgcggcgcgt acaacaaaat gtccaagttg ccgaaagatg cgctcgcttg cggcgtgatt
     1381 gcggcaagcg cgggcaatca cgcacaaggc gtggcgttgt ccgcacagcg tttgggctgc
     1441 cgtgccgtta tcgtcatgcc ggagactacg ccgaaaatta aagtggatgc ggttaaaagc
     1501 cgtggcggcg aggtggtttt gcggggtgtt tcatacaacg atgcctacga ttatgcgatg
     1561 gagctggcgg aacaggaagg gctgacctat atcgcgccgt ttgacgatcc cgatgtgatt
     1621 gcgggacagg ggacggtggg gatggaaatt gtcagccagc atcccgatcc aatccgcgcc
     1681 gtattcgtac cgataggggg cggcggtttg gcggcgggcg tggcggcatt tatcaaacag
     1741 gttcgtcccg aaattaaagt tatcggcgta cagaccaacg attcctgctg tatgaagcag
     1801 tcggtcgaag caggcgaaat cgtccatttg aaagatgtcg ggctgttttc agacggtacg
     1861 gcggtcaaag tcgtcggaaa cggaaccttc cgcctctgca aagaactttt ggatgaaatc
     1921 attacagtcg ataccgatgc ggtttgcggc gcggtcaagg atattttcga tgacacgcgc
     1981 agcattaccg agccggccgg cgcgttggcg ttggcgggtc tgaaagccta tattgcccga
     2041 gaaggtgcgg aaaaccaaac cctgattgcc gttaccagcg gtgcgaatat gaattttcac
     2101 cgtttgcgcc acgtttcgga acggagcgaa ttgggcgagg gcaacgaagg tatttttgcc
     2161 gttaccatcc ccgaagaacg cggcagcttc cttaagtttg tcaatatatt gggaaatagg
     2221 aatattaccg agttcaacta ccgctacggc ggcgatgaaa aagcgcatat ctttgtcgga
     2281 cttcaagcgg caggcccgca ggatttggcg gttatcggca gccggttgga tgaggcggga
     2341 ttgcccaatg tcgatttgac caataatgag attgccaaaa tccatatccg ctatatggtc
     2401 ggagggcgga cggacaaagt agggcacgag cgtctggtca gttttgagtt tccggagcgt
     2461 ccgggcgcgt tggcgcgctt tttgaaccat atgcagggcg gttggaacat tacgctcttc
     2521 cattaccgca accacggtgc ggattacggg cggattttgg tcggtatcga cgtgccgccg
     2581 cacgatgccg ccgcatttga cggtttcttg gaaagtctgg gatacagcta tcacgaggaa
     2641 acgcaaaatg ccgcgtacaa gctgtttctt gcctgacgtt tgacacgcaa tgccgtctga
     2701 aagcctttca gacggcattg cgctttcagg gttaaatcga atattcaatc aactcgtttt
     2761 gagaaaaaac ataaacctgt ttcggaatta agtttaattt tttgccttcg gcgattgggt
     2821 agcgcgcggc atcgctgcca gccagtgtga tatgcacgtc ttgtttgccg tgtttcacca
     2881 aaacgtgcgt caatgcgccg acggcgtgga ttttttcgat ttcggcacgg atcatcgggg
     2941 tttcgtgttc ggcggcgatc tgccattcgt gcgggcggat atagccggtg gcggtttgct
     3001 cctgccattt gtattgcgcg tccaatttcc acgcgaagcc gttgtaatgc cagatgcctt
     3061 tttcgatgcg tccttcaaat gcgtcggctt cgccgaggaa ctcggtaacg aaggcgtttt
     3121 cgggtttgcg gtaaatggct tcggtactgc cggtttgttc gattttgcca tggttcatca
     3181 cgacgatttc gtcggaaact tcgagggctt cttcttggtc gtgagtcacc agaatgctgg
     3241 ttacgccgag gttgtgatga atgtcgcgca gccaggtgcg taattctttg cgcactttgg
     3301 catccagcgc gccgaagggt tcgtccaaaa gcaggagttt cggttcgacc gcaagcgcgc
     3361 gggcgagggc gatgcgctgg cgttgcccgc cggagagttg gtgcggatag gatttggcga
     3421 ggtgggagag ttgtacgagt ttgagcaatt cttcgacttt ggcgcggatt tgtcctttgg
     3481 acgggcgttc gggcttgggc aatacggtca aaccgaaagc gacgttgtca aacacattca
     3541 tgtggcggaa gagggcgtag tgttggaaca cgaaaccgac tttgcgctcg cgcacatgct
     3601 tggcggttac gtcttggccg tcaaagagaa tttggccgcc gtcggcgttt tccagtccgg
     3661 cgataatgcg taaaagtgtg gttttgccgc agccggaggg accgagcagg gaaacgagtt
     3721 tgccggcggg gacgttgagg ttgatgtttt tcagcgcgtg aaaattgccg aagcgttttt
     3781 tgtttaagtt ttgaatggta atactcatac tgcgttcctt tcggcggcgg cgagtttttt
     3841 gtcttgtaat ttggtgatga tgttctgcac cgccagcgtc gccagtgcta aaagtgccaa
     3901 tacgccggag agggagaatg cgccggtgaa gttgtattcg ttgtagaaga tttcgaccaa
     3961 aagcgggata gtgttggttt cgccgcgtat gtgtcccgat accacgctga ccgcgccgaa
     4021 ctcgcccatc gcacgggcgt tggtgaggat gatgccgtag agcagcgtcc atttgatgtt
     4081 gggcagggta acgcgccaaa acatctgcca accgcttgcg ccgagtatca atgccgcctg
     4141 ttcttcgctg tcgccctgtg cctgcattaa agggatgatt tcgcgtgcgg caaagggaaa
     4201 ggtaacgaac agcgccgtca aaacaatgcc gggaatggcg aagataatcc gtatgccttg
     4261 cgcttccagc cggctgccca acgccgtgtg cgcgccgaac aataagacga acatcaaacc
     4321 ggcaaccacg ggcgatacgg aaaacggcaa atcgagcagg gtggtcagca actgcttgcc
     4381 gagaaagtca aaacggatca acagccacgc catcgccacg cccaagacgg cattgacggg
     4441 aacgacaatc agcgcggtaa tcagcgtcaa tttgacggca gaccacgctt cgggatcgct
     4501 taaggatttc aggtacaaat tccaaccgcc ttttaaggct tcgtaaaaca cggcgacgag
     4561 cggcacgacc agcatcagca gcagaaagcc cagcgcggcg gcaatcagca acatgcgcag
     4621 ccggcgcggt tcggtcaggt tgggattggc ggaatagggt ttcatggcgg tggtgttctc
     4681 tggtttgttg tttaaggtgt tcggatgccg tctgaaaggg attgaggggt ttcagctttc
     4741 gtcggtcgga ttcttgaatc cgacaaggtt gctgaatcgc aaatgtttgt cggatacaag
     4801 tatccgacct acgaattctg tgttttgggg agcggattcg ttttcagacg acctcaaccc
     4861 ttcgcacccg aacgcctgcc caacgcccac tgcatcacgt tcagcgcaaa cagaatcaca
     4921 aacgaaacca gcagcataaa caacgccacc gccgacgcgc cctgcacgtc gaattgttcc
     4981 agcttgcccg taataatcag cggcaggatt tcggaaatca tcggaatgct gcccgcgata
     5041 aaaatcaccg aaccgtattc ccccgttgcc cgcgcgaaca tcatgcccgc gccggtcaag
     5101 agcgccggcg tgatttccgg cagcagcacg cggcggaacg tcgtgaaacg gtttgcgccc
     5161 aaagtcgccg ccgcttcctc atattcgccc gacaattcct tcaataccgg ctgcacggcg
     5221 cggacgataa agggcaggct gacgacaacc agcgcaatcc aaatgccgac gggcgtaaac
     5281 gcgattttga tgcccaaagg ttcgaaaaaa cggcctatcc aaccgttggg cgcatacagg
     5341 gccgccaacg cgatacccgt aaccgccgtc ggcagcgcaa acggcaaatc aaccagcgcg
     5401 ttcgccaggc ccttgcccgg aaattcatag cgcaccaaca cccaagccac cagtgtgccg
     5461 aacacgacat tggtcagcat cgcataaaac gacatccgca aactcagccg caccgccgcc
     5521 agcacgttcg gctcggcaat cgtgttccga aagccgcccc agccgatttc cgccgccatc
     5581 atcgcaaacg gcaaaaccac cagaagcgac aggcacaata cggtcagacc gaggctgagt
     5641 ttgaagccgg gcagtacgcc gggtgttttg agcggtaaca tggtcggtaa gggtggaaaa
     5701 taaaggcatt actttaacaa tgccgcctga aaaacggaaa aaatggaaag tttggtgcaa
     5761 agacgaattt gttataaagc ggttggcagt ttttcaagcg ggcgcgatgt tctaaaataa
     5821 cgctttgttt tgacggaaaa ccatcatata aaggaacact tatgcagatt ttatcttttc
     5881 aggcggacat tgcggaacgt atgctggaag gtacggaagg cgagtcggtc aacgaaaacg
     5941 cacaatttgt ccgtacggac aacggctatt ggattgcgtg gcatgaaggc gtggcggcac
     6001 tgcttgcgcc cgatacgccg ccgggcattc cctgtttttg ggtggaaggg gcggaaagcc
     6061 ttgaagagtt gtgcgcgatg gtggaacgcg gcgagtttga cgaagtggaa gagtttgacg
     6121 gcgatgacga cgcgtggctc gaagcggcta aagattgcgg acaccacggc gacgcttgcg
     6181 cctgcggaca ttaaaggcat ggcaggcttg ccgcaagggg tgcaaggctt tgccgttttt
     6241 taaataatcg gcggattgca tctgtttgca atgggcggaa cgcttccctt tgtattttat
     6301 tgaataaaaa ttatcttggt attataataa ggcagcatca attattttgg gactgcaaca
     6361 aacgcaaagc gttgatttgc gccgattgca aacttattat agcaggttgc ggcgcggact
     6421 tgatgggaat tgtttataga gcatgatagt ctgaaacata ttaattaaaa ataccattaa
     6481 tccgatttat atttatttca atttcaatgg aaaaacatca atgacaatga ttttaagcat
     6541 tttaagcctg ttttttatca ttagactgtt atttttagcc gtctctatta aacatgaaaa
     6601 agccttgatt gccaaagggg cgaaacaata cggaaaaacc aattccacgg tgcttgcggc
     6661 agttcatacg ctttattatt tggcgtgttt tgtttgggta tggctttctg acactgcttt
     6721 taatggcata tccttgattg gtacactgac ggtgatggct tcatttgtga tattatcatt
     6781 gattattaag cagttggggg agatttggac ggttaaaatc tatattttgc caaatcatca
     6841 aattaatcgt tcgtggttgt ttaaaacatt ccgccacccg aattattttt taaacatcat
     6901 acccgaactg attggcatcg ccttattatg tcaagcgtgg tatgttttat tgattggcct
     6961 gcccatttat ttgctggtct tatttaagcg tatccgacaa gaagaacagg cgatggcaac
     7021 acttttttaa cccgtttcat caattaaaaa tatatcgaca aagagaaccg tctatcaatt
     7081 aaatccattc ttatcattgg ggcaggctta ttgtcattgg gcaagccgtg acaaaagccc
     7141 gaacctgcaa aacttatcta taaggctgca tcagtgcgta ttgaagcccc gccttttttg
     7201 acaaaaaaat gccgtctgaa ggcttcagac ggcatttgtt cggtttggca aagccggcgg
     7261 cttatgcggc aaaacgtttg gcgacttcgt cccagttgac gatttcccaa aaacctttca
     7321 ggtagttggg gcggctgttg cggtagtcga tgtagtaggc gtgttcccac acgtcgcaag
     7381 tcaagagcgg cgtgttttca gtggtcagcg gagttgccgc gttggaagtg gaaaccaaag
     7441 ccaatccgcc ggcaggggtt ttcaccagcc acgcccaacc ggatccgaaa gttccggccg
     7501 cgcaggcatt gaacgcttct tggaatttct cgaagctgcc ccattcggca tcgatggcgg
     7561 cggccagttc gccggcaggc ttgccttggc ctttgggcgt gaaacccagc cagtagaagg
     7621 tgtggttcca agtttgagcc gcgttgttga acacgccgcc cgaagatttt ttcacaatct
     7681 cttccaaagg caggttttca aattcggtgc ctttgatttg attgttcaga ttggtgatgt
     7741 aggtttgatg gtgtttgccg tagtggaatt ccaaagtctc tttgctcaga tgcggggaca
     7801 atgcgtccgg ctcataaggc agttgcggca gcttatgttc cattttgtgc tcctaatgtt
     7861 gtttttggat gttgtgtttt ggcagtgttg ctgcaaacga atcggcggct cacgcattct
     7921 acctgttttg agtgatggaa accaattaaa cctgctttgc gttataatag aagattgcaa
     7981 tttcggcatg acagattgga tgtaccatga acgattacgc agctatgccg cctgaaggcc
     8041 gtgaggtcgg cgcattgtcg ctgcctccgc attcgatgga ggcggaacaa tccgttttgg
     8101 gcggtttgat gctggaaaat ccggcttggg acaggattgc cgatgtggtt tcgggagagg
     8161 atttctaccg ccacgaacac cgcctgattt tccgatccat taccaaatta atcaatgagg
     8221 gccgtcccgc agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag
     8281 cggcaggcgg atttgaatat ctgattacac tggcgcaaaa caccccgtct gccgccaaca
     8341 tccgccgcta cgccgaaatc gtacgcgagc gttccattat gcgccaactc gccgaagtgg
     8401 ggacggaaat cgcccgcagc gcatacaatc cgcaaggcag ggacgcgggg cggcttttgg
     8461 atgaggcgga aaacaaagtg tttcaaatcg ccgaaagcac cgccaaatcc aagcagggct
     8521 ttttggagat gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctttattcgc
     8581 gcgacaatcc cgatgaagtt accggcgtac cgacagggtt catcgacctt gataaaaaaa
     8641 cctcgggtct gcagcccggc gacctgatta tcgtcgccgg ccgcccgtct atgggtaaaa
     8701 ccgcgttctc catcaatatt gccgagtatg tcgccattga aaaacatttg cccgtcgccg
     8761 ttttctcgat ggaaatgggc ggggcgcaat tggtaatgcg tatgctcggt tcggtcggac
     8821 gcttggatca gagcgtcctg aaaacaggca ggctggaaga tgaacattgg gggcgtttga
     8881 acgaggcagt cgtcaaactt tccgacgcgc ctatgtttat tgacgaaact ccgaatctga
     8941 ccgccctcga attgcgcgcc cgcgcccgcc gcctcgcccg ccggtttgac ggcagattag
     9001 gcttgatcgt catcgactac ctgcaactga tggcggcatc cggccgttcg gacaaccgcg
     9061 cttccgagct tggcgaaatt tcccgttccc tcaaagccct ggcaaaagag ttgcaagtcc
     9121 ccatcatcgc cctgtcgcaa ttgagccgca ctgtcgaaca gcgcaccgac aaacgcccga
     9181 tgatgtccga cctccgcgaa tccggtgcta tcgagcagga tgccgacctg attatgttca
     9241 tgtaccgcga cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca
     9301 tcggcaaaca ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca
     9361 ccaaattcga caatgctgcc tatattcccg aggaggcaaa gatagaagat taaatggcta
     9421 tataaaaatt tattaggcgg aatcaggcaa aatcgtttaa aatcatgctt agagattgcc
     9481 ctaaaaaata aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaaat
     9541 atagtggatt aacaaaaatc aggacaaggc ggcgggccgc agacagtaca aatagtacgg
     9601 aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgggg
     9661 caacgccgta ccggtttgaa tttaatccac tatacattac caattaacgt cctattgctt
     9721 atgtgtacac gaaaacaaca aggtttcacg ctaacagagc tgctcatcgt gatggccatt
     9781 gcagccatta tggcgacgat agccctcccc aatatgagtg ggtggattgc atcacgccgc
     9841 attgccagtc acgcggagca ggttgccaac cttttgcgtt tttccagggg cgaagccgtc
     9901 cggctcaatc tccctgtcta tatctgtcct gcccaagtta aaaaagacgg tacgggcaac
     9961 aaccgatgcg acctcggtaa gaaggaacag ggaatgttgg ctttcggcga caaaaacggc
    10021 aataaggcat atgacggcga tgtggcggat gttttcctcc gcagcgtggt gttgaatgat
    10081 accgacgaca gccggattaa ttatgccttc aaccatatcg ctttcggttc gtctcagccg
    10141 accgccggcc gtgttgtttg gactttcaac caaaacggga cattcggcta tttgtccgat
    10201 cagaatctca aggataattc caaatttgtt tattctgacg gttatatcca aatcgtgctg
    10261 acagatgcga gagcagtttc agatgccgat aggaaattcc gttcggcggt ggttttgatt
    10321 gacagcagcg gcagggtcga agtttgtcgt aaaaacgata cgcgcgccgt atgcaaacat
    10381 taatgactgt tttagtcggt ttgggaaata aaaaatgaag aataatgatt gcttgcgcct
    10441 gaaaaatccc cagtccggta tggcgctgat agaagtcttg gttgctatgc tcgttctgac
    10501 catcggtatt ttggcattgc tgtccgtgca gctgcggacg gtcgcttccg tcagggaagc
    10561 ggaaacgcaa accatcgtca gccaaatcac gcaaaacctg atggaaggaa tgttgatcaa
    10621 tccgaccatt gatccggacg gcaacaagaa aaactataat ctttacactg agtcgtacac
    10681 ccccactccc tctggcggcg atttcaagct taataataat aatttgataa gtaagaagga
    10741 tttggcaaag gcccagttgg acaggttcgg ttatgaattg aaaaatgctt tgccggatgc
    10801 ggtagatatt cattacgctg tctgcaagga ttcgtcgggt aaggcaccga cattgtccgg
    10861 cggtactttt tttccaaatt gcgataataa ggcaaacggg gatactttga ttaaagtatt
    10921 gtgggtaaat gattcggcag gggattcgga tatttcccgt acgaatcttg aggtgagcgg
    10981 cgacaatatc gtatatactt atcaggcaag ggtcggaggt cgtgaatgaa acgtaaaatg
    11041 ctaaacgtac caaaaggcgg ttatgatggt atgaagggtt ttaccattgt tgaatttctg
    11101 gttgcgggcc tgctcagtgt gattgtcctg atagcggtcg tatcgagtta ctttacatcc
    11161 cggaaattaa atgatgcggc aaacgagcgt cttgccgagc aacaggattt gcggaatgcg
    11221 gcaacattga ttgtccgcga tgcaagaatg gcggggagct tcggttgttt caatatgtct
    11281 gagcatatcg gaaatgatgt tttttccaat gtggcgcaaa aaaacgctct tttttcctta
    11341 aaaaggaaca gtacaaacag tacaaataaa cttatcccca taacggaatc tccaaatatc
    11401 aattatcgga attttttcca ggttagtagc gcattgattt ttcaatacgg aatcgatgat
    11461 gttaatgcaa gcgccgcgac taccgtcgtc agcagctgtg ccgcaatatc gaaaccgggc
    11521 aagcaaatcc ctactttaga agatgcaaaa aaagaattga agattgtgag tcaggataag
    11581 gagcaaaatg gcaatatagc gcgtcaaagg catgtggtca atgcctatgc ggtcggcaag
    11641 attgccggtg agggaggttt gttccgcttc caattggatg ataagggcga gtggggtaat
    11701 cctcagttgc tcgtgaaaaa ggttaaacgt atgaaagtgc ggtatatcta tgtttccggt
    11761 tgtcctgaag atgaagatgc cggcaaagag gaacaattca aatatacgga taaattcgac
    11821 agctctgtta cgcctgccgg ggtggaggtt ttattggata gcggtaatga tgccaagatt
    11881 gccgcttctt cagataatat tatttatgct taccgtatca atgcgacaat acgcggggga
    11941 aatgtatgcg caaacagaac actttgacag gaatcccgac ttctgacgga cagagggggt
    12001 tcgcactgtt tatcgtgctg atggtgatga tagtcgtggc ctttttggtt gtaactgccg
    12061 cccagtccta caataccgaa cagaggatca gtgccaacga atcagacagg aaattggctt
    12121 tgtctttagc cgaggcggct ttgagggaag gcgaatttca ggttttggat ttggaatata
    12181 ctgcggatag taaggttaca tttagcgaaa actgtgaaaa cggcctgtgt accgcagtga
    12241 atgtgcggac aaataataat ggtaatgaag aggcttttgg caatatcgtg gtgaaaggca
    12301 agcccatcgt tgaggcggtg aagcgttctt gccctgcaaa ttctgccagc ctgtgcattg
    12361 acaataaagg gatggaatat aggaaaggca cggcaggcgt cagtaaaatg ccgcgctata
    12421 ttatcgaata tttaggcgtg aagaacggac aaaatgttta tcgggttact gccaaggctt
    12481 ggggtaagaa tgccaatacc gtggtcgtcc ttcaatctta tgtaggcaat aatgatgagc
    12541 aataaaatgg agcaaaaagg gtttacattg attgaggtaa tgatagttgt cgcgatactc
    12601 ggcatcatca gcgtcattgc cataccttct tatcagagtt atattgaaaa aggctatcag
    12661 tcccagcttt atacggagat ggtcggtatc aacaatgttc tcaaacagtt tattttgaaa
    12721 aatcccctgg acgataatca gaccatcaag agcaaactgg aaatatttgt cttagactat
    12781 aagatgaatc cgaaaattgc caaaaaatat agtgtttcgg taaggtttgt cgatgcggaa
    12841 aaaccaaggg catacaggtt ggtcggtatt ccaaagacgg ggacgggtta tactttgtcg
    12901 gtatggatga acagcgtggg cgacggatac aaatgccgtg atgccacttc tgcccaggcc
    12961 tattcggaga ccttgtccgc aaataccggc tgtgaagctt tctctaatcg taaaaaatag
    13021 ggttgttttg ccaataccgt ctgaagatca attttcagac ggtatttttt atgggcattt
    13081 tgaaatgaat tatgccatca accatcccga gatgcctgac gagccgaccc ctatcaatac
    13141 ggtaaacaac atagaaaaac ggcaggccgc aaaggcggtt aacgcgatgg caataagttc
    13201 gtgcggcttg tcggagacaa aatagggggc aatgacggaa atgaggacgc agcccggcgc
    13261 ggcttccata acggtttggg cgcggcggct taaggttcgg ttacgcagtg cgaaaaagcc
    13321 gattaaccgg gtagggcagg taacggcaag catacaggaa aacagcagga atgatgggga
    13381 cgacaataaa tctttcattt ccgcccattc caaaaatagg cggacaacag gccggacagc
    13441 gtgccggcgg gaacatacca cgcgccgtca acggtcaggt aaacggcgga tgcgacaaac
    13501 aggttgacaa atcaaggtat tgatgcggaa agatttttcc acattcccct cagtaaaacc
    13561 agaaatacgg caggaaacgc cataccgaac cctcaagcgg ccacacattg ccgaacacgg
    13621 gctctactgc cgtgccgagg gcggcaaacc ctatcaggta atgtagagga taaagcatgc
    13681 gcccatataa gaaggcatat tgaatgcagg caaaccggct gctttccgtt tttggatttc
    13741 ggagaatgcc atggcctagc tttcatcaca cataaaaaac agcgcgggcg cggctttttt
    13801 cagcggtatt tctttcatgt gcgtggcaag cgccgccccc catcaggata tgccgcgaat
    13861 taatcataaa ggttatggtg gtgataagca gtatcggcag aggggccgcc cacagattga
    13921 ccgttgcaaa ctctgagccg ccggcgaagt tcatgccggt catcagcagc atttccagcc
    13981 ggcccatacc tttttgcccg cctgcattcc gagtattaat gcccaaggca aaagcccaat
    14041 cagcatgggc gagctttctt ttatgccgcg tatgaattca tcacgtggag aggctgtgtg
    14101 cgtcatgtta atgtttatat atgaagagac ggggcggtat catacacagg cagaatgttt
    14161 ttgacagtcg aggatggttt caaaacaaaa gcagtttgtt gtgtttcagc gtaaacacgg
    14221 cttgtgtata atctccaatc tttgaaaccg gccgtatgca ggagcaggac gatgaatatt
    14281 gaagtagaaa tgaaagtgtt ggacgagagg atggcggatt ttatccctgc ctatgcgacg
    14341 gagggttctg caggtttgga tttgcgcgcc tgtttggatg aggaagtcgt tttgcagccg
    14401 ggcggaacgt tgcttgtgcc gacaggtttg gcaatttatt tggcgaatcc cgcatatacc
    14461 gccgttttgc tgccccgttc cggcttgggg cataaacacg gcattgtctt gggcaatttg
    14521 gtcggtttga ttgactccga ttatcaggga gaattgaagg tgtcgttatg gaacaggggc
    14581 agcgaaccgt ttactgtcaa accgttcgag cgtatcgcac aaatggttat cgtgccggtc
    14641 gtacaggcgg gcttcaaacg tgtcgaggaa tttgtcggaa gcagccgggg tgagggcggc
    14701 ttcggcagta cgggttctca ctaaagatat agaatgccgt ctgaagggcg cgtcagcttc
    14761 agacggcata tcttccgata taccgataac cggagaaaat catgaatacc ttactcaagc
    14821 agctcaaacc atatcccttt gcccgactgc gcgaggcgat gcagggcatt tccgcgcctg
    14881 aaggcatgga agccgtccca ctgcatatcg gcgaaccgaa acacccgaca ccgaaagtca
    14941 ttacggatgc gctgaccgcc tcattgcgcg agttggaaaa atatccgctg actgcgggtc
    15001 tgcctgaact gcgtcaggcg tgtgcaaact ggttaaaacg ccgttatgac ggcttgacgg
    15061 tcaatccgga taatgaaatt ctgccggttt tgggcagtag ggaggcgttg ttttcttttg
    15121 ttcaaaccgt attggatccc ggttcagacg gcatgaaacc cgtcatcgtc agccagaatc
    15181 ccttttatca gatttatgaa ggtgcggcag ttttgggcgg tggggaaatt cattttgcca
    15241 attgccccgc gccgtctttc aaccccgatt ggtgcagtat ttcccaagag gtgtggaaac
    15301 gcaccaaact ggtgttcgtc tgctcacccg gcaatcccag cggcagcgtg ccggatttgg
    15361 acggctggaa agaagttttt gatttacagg ataaatatgg tttcattatt gcctcggatg
    15421 aatgctattc cgaaatctat ttcgacggca acaaaccttt gggctgcctg caagccgccg
    15481 cacagtcggg gcgaagcagg caaaaactgc ttatgttcac cagtttgtcc aagcggtcca
    15541 atgttccggg cctgcgttcc ggttttgtcg ccggtgatgc ggaactgctt aaaaactttc
    15601 tgctttaccg aacctatcac ggcagcgcaa tgagcattcc cgtgcagcat gccagtattg
    15661 ccgcctggaa tgacgaacaa cacgtaatcg acaaccgccg tatgtatcag gaaaaatttg
    15721 agcgcgttat tcccattttg caacaggtgt ttgacgttaa attaccggat gcctcgtttt
    15781 acatctggtt gaaagtcccc gatggagacg atttggcatt cgcacgcaat ttatggcaaa
    15841 aagccgctat ccaagtattg cccggacgct ttttggcgcg ggataccgag caggggaatc
    15901 ccggggaagg ttatgtgcgg attgctttgg ttgccgatgt cgcaacttgt gtcaaagctg
    15961 cggaaaccat tgtttcccta tatcggtaaa gaataaaaaa taccgtccgg acttttcctg
    16021 acggcttttt ttaatactat caagatgtat ttattataac ggtatttaca tcacaatcaa
    16081 tcggcttgcc tgaggatgct tttaaatcta aaattccgac gtttgacact ataccgagcg
    16141 gattatgttt tttgtccttt cccctgcgaa aaaccttaac gaaaaagacc cttgccctgt
    16201 cagcgagttt acccaacccg atcttttggc ggaatctgaa atcctgatgc gccaactgag
    16261 ggagcttgcc cctcaacaaa ttgcagagtt gatgcacgtt tccgacaaaa tcgcacttct
    16321 aaacgccgaa cgaaatgcag cgtggcacac gccgtttacg ccggaaaacg ccaaacaggc
    16381 agtctttatg ttcaacggcg atgtttacga aggtatggat gcaaacacat tgaataccaa
    16441 tcaaatacaa tatctgcaaa accatgtccg cctgctgtcc ggcttgtacg gtcttatgcg
    16501 tccactggat ttgatgcagc cctaccgcct ggaaatggga acggcatttg ccaatttgcg
    16561 cggcaagaat ttgtatgagt tttggggcgg catcattacc aaccttttaa atgacacgct
    16621 tgcccaagca ggcagcaata cgcttgtcaa ccttgcctca caggaatatt tcaagtccgt
    16681 caacacgaaa aaacttcggg cgcggctgat taccccgata tttaaagacg aaaaaaacgg
    16741 taaatataaa atcatcagtt tctatgccaa acgcgcgcgc ggattgatgg tgcgctatgc
    16801 ggcagaacac aatattaccg atcctgaaat gctgaaaaat tttaattatg aaggctacgc
    16861 attcaatgac gcggcttcca atgaaagcga atgggtgttt atgcgttcgg aacaaataaa
    16921 gtgaaaacaa taaattaagt atttcccaaa aaaagtgctt ggcaaaatgt ataaacttca
    16981 ttattattcc tactcttcaa gaagacggaa gcgtggcaga gcggtttaat gcaacggtct
    17041 tgaaaaccgt cgagggttga tagccctccg tgagttcgaa tctcaccgct tccgccaatc
    17101 cttgaagtca aaaaccaaat aaaaatgaag tagcataaag catcagcatt tttatttggt
    17161 tttacttcat tatcggaaac gtggcagaga ggctgaatgc agcggactcg aaatccgctg
    17221 agggtgcaaa tcctccgtgg gttcgaatcc cgccgtttcc gccgcaaagc aaaaccgccc
    17281 tgattcaggg cggttctttt ttgttcaggt tgtgttaatt actatataaa aatcatcggt
    17341 ttaccctatc ataacgcatc aaggtaaatc atttgcaatc ttgcggcatc ttcttattgc
    17401 atttttttat ggtaatgtat atggtaattt ttggataagt gggaaattac cataatggcg
    17461 aaaatcatta cgccgctgtc ggcaaatcag gttaaaaatg cgaagccgcg cgataagctg
    17521 tataagttgt cggacggcgg agggttggct ttgtgggtct atccgacggg caggcggagt
    17581 tggaagctgt cgtttatgca ggacggaagg caacagacaa tttctctggg gcggtatcct
    17641 gatttctcgc tggctgaggc gcgggaatgg cgggaggagg tgcgccgaaa acgggcacac
    17701 ggggaaaatg tcgtcaataa gaaggtgcgg gcggattttg cttttgagaa ggtggcgcgt
    17761 gattggtttg tgcgttggtc gaaggggcgg tctgaaaagt atgccggaca ggttatgcgg
    17821 aattttgaac ggtgggtttt tccggctatc ggcaatcttg atatccgtca agtcaggacg
    17881 gcggacgtgg tcggctgcct gcgtgtgatg gaggcgcgcg gtatcgttga tacgttgcgc
    17941 aaaacgaaaa acagtctgaa gatggtgttt gcgtttgcgg tcggttcggg aatgatggac
    18001 atcaaccctg tcgcgcaaat cggttcgggt gtgtttgaac gggtgaaaac caaaaacatg
    18061 acggcgttga gtccgtccga attgccgcgc ctgattgatt ttttggagca gcgcaatgaa
    18121 tttgcggttt atgcgggcag ggtgcatatc catcctgtaa cgcggttttg tatctattgg
    18181 ctgctgttga cgatgacgcg gattcgggag gcggcgttga tggagtggtc ggagttggac
    18241 ggggaggttt ggcgtatccc cgccgaacgg aaaaaggagc ggcgggggca tgatgtgccg
    18301 ctgtcgcggg cgatgcggtg ggtgttggat caggcgcggg ggctgaatgt gaacgggcgg
    18361 tttgtgtttg aaagtgtgaa ttttcaaggg catatcaata aggaaagtcc gcacgtggcg
    18421 atgcggcggt cggggctgga tacgacggcg cacggtttgc gctcgcttgc gcgtacttat
    18481 ttgcgcgagg ttctgaaggt gtataatgat gtggcggaaa agctgcttgc ccattcgttg
    18541 gggacgagga cgcaaacggc ttacaaccgc tcggagcttt gggaggagcg taaggatgcg
    18601 ccggaacggt gggggaatga tgttttgagg cttgccgaca acggaaaatg atttttttgt
    18661 gtgttttgcg ttatgtggaa atgtagataa gaaagctgtt tccgcattat gtgaaaacgc
    18721 cctaatcggg cgttttttta ttgctgcaac cctaatgctt ccaaaacttc gcgggtgtcc
    18781 catacggggg atgcgaggga ggttcagacg gcacggtatc cgtccgtctt tttccatacg
    18841 cagcagtgtc gagtttgaaa tggggtggtt gcggcaggtt gcgtaggcaa tcagttcgcg
    18901 gatggtcggg cggtctatcc ttgcgcccgg tttggtgatg ttcatttttt tactctcctg
    18961 ttcggtttaa tcttgcgccg tttttacggc tctgtcgttt gtctgcctat ttcttcttcc
    19021 cgattttttt atatcgcaac aagctttccg gcgggaagaa tatgtcaaag tctgttttca
    19081 aatcgggact gacgatgtcg gcggcaatca cgccgtagcc ggtggcaaag ctgtcgccat
    19141 cagcgttatg aataactata tgcccgcttt tgattagaat aacgcggccg aatccggatt
    19201 tccggattcc gccttttttc ggggcggttg caaatgccag tgtttccatc cgcccttgta
    19261 cgtcggaaga ctccattgct tcgccgaccg cttttttgac gggcgtttat tgaatacttc
    19321 gtattccatg tcttcaatcc tctttccgtt gattccgatt tgtccgggtt tccgcttttt
    19381 tacggcttgg catgcggcaa ttgcgtcttt catattcggc atatcctttc cccaatccgc
    19441 cgaagtttcc gcatccgccg ccgtctcgat tcatgtttgc cctccgattt gttttacgaa
    19501 gcccgtaccg ttcacggcat agtgtaactt cacttgctta cccgattcca ttcctcgggt
    19561 taatgcctca tattccattt ttgacccttg taggattgac gatttcgcat ctcttgtcct
    19621 tgcttcggcc atatccggat attctttctt tttgtatccg tccataatgg ctttttcttc
    19681 atctgacatc tcaaattttt tgactgtttc ccaagcgctc gcaatccaac cgccgcaaaa
    19741 tcggtcggca agataagttc tgtgcgaggg ctttcccgcc cggcaggttt tcaaaaattt
    19801 gcggcggtcg gcggaaatct gacgatagac tacatcaaag gcataggctg aggtttccgc
    19861 tcgatttcca atgccgtaaa acatcattgt tttcccgcgt tggtaagatt tgcacccgaa
    19921 tatatcggca atcatatttg caacgcccca ttgccagcct gccaatttaa aagccatttt
    19981 ccgatcggct ttctgttcgg agacttttga taaggcgata tcctcggcat ttactttgta
    20041 cttttccatt aaagcctgcg cctgccttaa cgcctgtgcg gcttcatgtt catttgccga
    20101 ccgacccaaa gccaaacatt tcttgatttt gtctaagact ttctctttat ccatattaaa
    20161 ccctttaatt tgtccggtat tttaatttcg cacccttgcc cattcgtctt tcgctttgct
    20221 ttcgattcgg tcgggccatt cggctagggc tgatacgctt acgaactcgg ggcttttttt
    20281 gtttccgcct gccgagatta ccggccgcgg cagggtagtt ttgcaacaag gctttgcagg
    20341 cttcgggctg tatttccacg gcgtgaaatt cagacggctt aatataccgt tcaagctgcc
    20401 tgcttcctgc cgcgccgtca aatacgcccg gatgttcgcc gcagtatcgg cggactttgg
    20461 cggcaaccag ccgcatcatg atgccgcacg tcagggcttc gtcttccgat acctttgcgc
    20521 cagacaacat ccgggcgatg ttttcttttt gcgcttttga ccgggcggac agccggttcc
    20581 ggtcaacgtt tctgaccgtc ccggcgcgtt tgacggcgcg ttcctgccgc gttgattcct
    20641 tcgccgcgcg tttggcggca agcatctgtt ttgccgtcgg ttttgttgtt gccgtttgca
    20701 tttttacctc cttttaaaac tgtttcgaca ggcttgcgaa cggccttccc gttcacttcc
    20761 cgcgcctgcc tgattcctac ggtgcggctg tcgggattgc cctccggcag cctgacaaac
    20821 ccttcggcgg cccctacggc cggatatccg gggctgattc ggcagcggct gttgcttggc
    20881 cgctcccgtc cgcgccggtc tgcgtaccgc ttggaatcct ctttgtcgca aacaggccgc
    20941 cgccgttttt cttcttcggg acgatgtttt ccaaaggctg cgaacatggc ggcccctgtt
    21001 tatctaatct gcaaattctg acgctcttca atcttcgcgc ctgctacttg ccgaccgctt
    21061 tcaatcgctt ttctgatggc gg
//
LOCUS       DAXFHF010000038        20794 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXFHF010000038 DAXFHF010000000
VERSION     DAXFHF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20794
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.038"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            215..2983
                     /gene="tdfH"
                     /locus_tag="ACFIRM_001265"
     CDS             215..2983
                     /gene="tdfH"
                     /locus_tag="ACFIRM_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC3028889.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAERVPKDKKVFTDARAVSTRQDVFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEEHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTRIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGAKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTTGANTSDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            3014..3229
                     /locus_tag="ACFIRM_001266"
     CDS             3014..3229
                     /locus_tag="ACFIRM_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028890.1"
                     /translation="MPSESPSDGICFPKRTILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(3607..3783)
                     /locus_tag="ACFIRM_001267"
                     /pseudo
     CDS             complement(3607..3783)
                     /locus_tag="ACFIRM_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(4009..5226)
                     /locus_tag="ACFIRM_001268"
     CDS             complement(4009..5226)
                     /locus_tag="ACFIRM_001268"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC3028891.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(5428..6156)
                     /gene="rph"
                     /locus_tag="ACFIRM_001269"
     CDS             complement(5428..6156)
                     /gene="rph"
                     /locus_tag="ACFIRM_001269"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC3028892.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(6238..6702)
                     /locus_tag="ACFIRM_001270"
     CDS             complement(6238..6702)
                     /locus_tag="ACFIRM_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC3028893.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            6920..7696
                     /locus_tag="ACFIRM_001271"
     CDS             6920..7696
                     /locus_tag="ACFIRM_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3028894.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            7700..7831
                     /locus_tag="ACFIRM_001272"
     CDS             7700..7831
                     /locus_tag="ACFIRM_001272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028895.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            7941..8795
                     /locus_tag="ACFIRM_001273"
     CDS             7941..8795
                     /locus_tag="ACFIRM_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC3028896.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVILGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            8836..10044
                     /gene="pncB"
                     /locus_tag="ACFIRM_001274"
     CDS             8836..10044
                     /gene="pncB"
                     /locus_tag="ACFIRM_001274"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC3028897.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEAGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            10136..11854
                     /gene="argS"
                     /locus_tag="ACFIRM_001275"
     CDS             10136..11854
                     /gene="argS"
                     /locus_tag="ACFIRM_001275"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC3028898.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGIAQTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            12409..13827
                     /locus_tag="ACFIRM_001276"
     CDS             12409..13827
                     /locus_tag="ACFIRM_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3028899.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            13833..14093
                     /locus_tag="ACFIRM_001277"
     CDS             13833..14093
                     /locus_tag="ACFIRM_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028900.1"
                     /translation="MPSERTNSLQTAFCRQTDLLKPTFFIISDKLLCKNPKIQNHRKL
                     SKKQKPSAVVPAKAGIQTRKPQKFIGKTETRKQRTEFPPAQE"
     gene            complement(14280..15026)
                     /locus_tag="ACFIRM_001278"
     CDS             complement(14280..15026)
                     /locus_tag="ACFIRM_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3028901.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(15010..15189)
                     /locus_tag="ACFIRM_001279"
     CDS             complement(15010..15189)
                     /locus_tag="ACFIRM_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028902.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(15205..15876)
                     /locus_tag="ACFIRM_001280"
     CDS             complement(15205..15876)
                     /locus_tag="ACFIRM_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC3028903.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDALGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(15945..16616)
                     /gene="rpiA"
                     /locus_tag="ACFIRM_001281"
     CDS             complement(15945..16616)
                     /gene="rpiA"
                     /locus_tag="ACFIRM_001281"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC3028904.1"
                     /translation="MTTQDELKRIAAEKTVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(16693..17175)
                     /gene="ispF"
                     /locus_tag="ACFIRM_001282"
     CDS             complement(16693..17175)
                     /gene="ispF"
                     /locus_tag="ACFIRM_001282"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC3028905.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(17207..17896)
                     /gene="ispD"
                     /locus_tag="ACFIRM_001283"
     CDS             complement(17207..17896)
                     /gene="ispD"
                     /locus_tag="ACFIRM_001283"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC3028906.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALARLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(17893..18627)
                     /gene="dnaQ"
                     /locus_tag="ACFIRM_001284"
     CDS             complement(17893..18627)
                     /gene="dnaQ"
                     /locus_tag="ACFIRM_001284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC3028907.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRVGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEETTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(18783..20093)
                     /locus_tag="ACFIRM_001285"
     CDS             complement(18783..20093)
                     /locus_tag="ACFIRM_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3028908.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(20083..20268)
                     /gene="ccoS"
                     /locus_tag="ACFIRM_001286"
     CDS             complement(20083..20268)
                     /gene="ccoS"
                     /locus_tag="ACFIRM_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC3028909.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(20268..20567)
                     /locus_tag="ACFIRM_001287"
     CDS             complement(20268..20567)
                     /locus_tag="ACFIRM_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028910.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
BASE COUNT         5004 a         5914 c         5160 g         4716 t
ORIGIN      
        1 caataccgca atctcgaaat tcgtcattcc cgcgcaggcg ggaatccgga cccctgacgc
       61 ggcgggaatc tatcggaaat aactgaaacc ccgcgtccta gattcccact tccgtgggaa
      121 tgacggttcg gttgcgtagg gccgggtggt cgaaaaggcg gattcgatga aaacggtaga
      181 catgttggat tgattgttta ttgttgagga aaatatgaga tcttctttcc ggttgaagcc
      241 gatttgtttt tatcttatgg gtgttatgct atatcatcat agttatgcag aagatgcagg
      301 gcgcgcgggc agcgaggcgc agatacaggt tttggaagac gtgcacgtca aggctgagcg
      361 cgtaccgaaa gacaaaaaag tgtttaccga tgcgcgtgcc gtatcgaccc gtcaggatgt
      421 gttcaaatcc ggcgaaaacc tcgacaacat cgtacgcagc atacccggtg cgtttacaca
      481 gcaagataaa agctcgggca ttgtgtcttt gaatattcgc ggcgacagcg ggttcgggcg
      541 ggtcaatacg atggtggacg gcatcacgca gaccttttat tcgacttcta ccgatgcggg
      601 cagggcaggc ggttcatctc aattcggtgc atctgtcgac agcaatttta ttgccggact
      661 ggatgtcgtc aaaggcagct tcagcggctc ggcaggcatc aacagccttg ccggttcggc
      721 gaatctgcgg actttaggcg tggatgacgt cgttcagggc aataatacct acggcctgct
      781 gctaaaaggt ctgaccggca ccaattcaac caaaggcaat gcgatggcgg cgataggtgc
      841 gcgcaaatgg ctggaaagcg gagcatctgt cggtgtgctt tacgggcaca gcaggcgcgg
      901 cgtggcgcaa aattaccgcg tgggcggcgg cgggcagcac atcggaaatt ttggtgaaga
      961 atatctggaa cggcgcaaac agcaatattt tgtacaagag ggtggtttga aattcaatgc
     1021 cggcagcgga aaatgggaac gggatttgca aaggcaatac tggaaaacaa agtggtataa
     1081 aaaatacgaa gacccccaag aactgcaaaa atacatcgaa gagcatgata aaagctggcg
     1141 ggaaaacctg gcgccgcaat acgacatcac ccccatcgat ccgtccggcc tgaagcagca
     1201 gtcggcaggc aatctgttta aattggaata cgacggcgta ttcaataaat acacggcgca
     1261 atttcgcgat ttaaacacca gaatcggcag ccgcaaaatc atcaaccgca attatcaatt
     1321 caattacggt ttgtctttga acccgtatac caacctcaat ctgaccgcag cctacaattc
     1381 gggcaggcag aaatatccga aaggggcgaa gtttacaggc tgggggcttt taaaagattt
     1441 tgaaacctac aacaacgcga aaatcctcga cctcaacaac accgccacct tccggctgcc
     1501 ccgcgaaacc gagttgcaaa ccactttggg cttcaattat ttccacaacg aatacggcaa
     1561 aaaccgcttt cctgaagaat tggggctgtt tttcgacggt ccggatcagg acaacgggct
     1621 ttattcctat ttggggcggt ttaagggcga taaagggctg ctgccccaaa aatcaaccat
     1681 cgtccaaccg gccggcagtc aatatttcaa cacgttctac ttcgatgccg cgctcaaaaa
     1741 agacatttac cgcttaaact acagcaccaa tgcaatcaac taccgtttcg gcggcgaata
     1801 tacgggctat tacggctcgg aaaacgaatt taagcgggca ttcggagaaa actcgccggc
     1861 atacaaggaa cattgcgacc cgagctgcgg gctttatgaa cccgtattga aaaaatacgg
     1921 caaaaagcgc gccaacaacc attcggtcag cattagtgcg gacttcggcg attatttcat
     1981 gccgttcgcc ggctattcgc gcacacaccg tatgcccaac atccaagaaa tgtatttttc
     2041 ccaaatcggc gactccggcg ttcacaccgc cttaaaacca gagcgcgcaa acacttggca
     2101 atttggcttc aatacctata aaaaaggatt gttaaaacaa gatgatatat taggattgaa
     2161 actggtcggc taccgcagcc gcattgacaa ctacatccac aacgtttacg ggaaatggtg
     2221 ggatttgaac ggggatattc cgagctgggt cggcagcacc gggcttgcct acaccatccg
     2281 acaccgcaat ttcaaagaca aagtgcacaa acacggtttt gagctggagc tgaattacga
     2341 ttatgggcgt tttttcacca acctttctta cgcctatcaa aaaagcacgc aaccgaccaa
     2401 tttcagcgat gcgagcgaat cgcccaacaa tgcgtccaaa gaagaccaac tcaaacaagg
     2461 ttatgggctg agcagggttt ccgccctgcc gcgagattac ggacgtttgg aagtcggtac
     2521 gcgctggttg ggcaacaaac tgactttggg cggcgcgatg cgctatttcg gcaagagcat
     2581 ccgcgcgacg gcagaagaac gctatatcga cggcaccacc ggggcaaata ccagcgatgt
     2641 ccggcaactg ggcaagcgtt ccatcaaaca aaccgaaacc cttgcccgac agcctttgat
     2701 ttttgatttt tacgccgctt acgagccgaa gaaaaacctt attttccgcg ccgaagtcaa
     2761 aaatctgttc gacaggcgtt atatcgatcc gctcgatgcg ggcaatgatg cggcaacgca
     2821 gcgttattac agctcgttcg acccgaaaga caaggacgaa gacgtaacgt gtaatgctga
     2881 taaaacgttg tgcaacggca aatacggcgg cacaagcaaa agcgtattga ccaatttcgc
     2941 acgcggacgc accttcttga tgacgatgag ctacaagttt taaaggcagc ccgcgttttg
     3001 tggaaaaact gcaatgccgt ctgaaagccc ttcagacggc atttgtttcc ccaaacgcac
     3061 catcctgccg caagcctatg ccaatccgtt ttatcgcatc ggcaactcaa agaaaaatcc
     3121 atttcattcc cacgcaggga agccggtttt tgatttcggt tatttttggt tgtttcgggt
     3181 aatttatgag tcgtcattcc cgcaaaagcg ggaatcagtt tttttatagt ggattaacaa
     3241 aaacaggtac ggcgttgcct cgccttgccg tactatttgt actgtctgca gcttcgtcgc
     3301 cttgtcctga tttttgttaa tccactataa atttcagcca tttccgataa attcctgtgg
     3361 ctttagcttt ccggattccc actttcgtga gaatggcggc ggagaggttt ctgtttttcc
     3421 gataaatccc acaacctaaa atctcatcat taccgcgcag atgatatgtt gcccgtcaac
     3481 accaaataaa aataaaaccg taataaattg atttatgttg ttatttttta tttactgtat
     3541 gcaaatgcac ggttacaaag atatatttgc ataactgttt aattttattg aattttattg
     3601 attcaatcag tgtctttccg catcgtaagg ctgcccggtt ttaacaatgt aataggcggg
     3661 cttcgccagt ttgcgcatga tggcaacgat gattaccatc tttggcttac ccgctttttt
     3721 cagattattt attaatttcg gaaatgcgtt aaaacggtaa gcacaaagga cgggcatata
     3781 cagcgggaat ccgttttttg agtttcgctt aagttcaata aatcctgaaa ctttaatctc
     3841 gccgtgaaaa tgaattttct atttcaattt gtcatatata gttaattttt ttagggtatt
     3901 ttattttttg ttccaaagtc aaaatatgcc gtccgaacgt tcgggcggca gacaaaacgg
     3961 cactgcccga taaaggcagt gccgttgtcc gttttaaacc gtgaaacatc agcccaaatc
     4021 aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcgtcaa tcaatacgga
     4081 aactttgatt tcggaggtgg aaatcatttg gatgttgatg ccctcttcgg cgagcgtgcg
     4141 gaagattttg gcggctacgc cgacgtgcga acgcataccc aaaccgactg cggagacttt
     4201 gcacacggtg tcgtcgccgt cgatagaagc tgcgccgata ctgtctttac gttccgacag
     4261 gatttccaaa gtctgtttgt aatcgccgcg cggtacggtg aaagaaaaat cggttgtgcc
     4321 ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatctg cgaccgcgcc
     4381 caaaatctga taggcgacgc cgggcttatc gggcacaccg cgcacgttga tgcgggcttg
     4441 gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt cctcttcaaa
     4501 ggtaattaag gtgccgttgc cgccgtcttg caggctgctc agtacgcgca ggcgcacttt
     4561 gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga ggctggccag
     4621 ttcgatcatt tcttcaaatg taaccgtatc catacggcgc gcttcgggta cgacgcgggg
     4681 gtcggtggtg taaacgccgt ctacgtcggt atagatttgg cattcgtccg ctttgagggc
     4741 tgcggcaagc gcaacggcgg aagtgtcgga accgccgcgt cctagcgtgg aaatattgcc
     4801 ttcactgctg atgccttgga agccggcaac gatgacgact ttgccggcgg cgaggtcggc
     4861 gcgcattttt tcgtcatcaa tgctttctat gcgggctttg gtgtgggcgg tatcggtttt
     4921 gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatatcct tcaatgccat
     4981 tgccaaaagg ccgatggtta cttgttcgcc ggtagctaag acgacgtcca gctcgcgcgg
     5041 atcgggatgc tcctgcattt cgtgcgccaa cgcaaccaga cggttggttt cgccgctcat
     5101 ggcggatacg acgacgacga tatcgtgtcc ttcggcgcgg gctttagcga cacgtttggc
     5161 cacgttttta atgcgctcgg gcgagcctac ggatgtgccg ccgtatttat gtacgattaa
     5221 cgccatgttt catgctttct tgtgtgggat tggtcgggca gcatggtttg ctggaaaagc
     5281 ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc ccgcctgccg
     5341 tttgacagcg gtcagcgaaa aacctgttct ttcagatttt tgacaaaatg ccgtctgaac
     5401 ggttttcaga cggcatacgg acagtaatta agcaacggac aacgcatttt gctgataacg
     5461 cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaact cgtccaaact
     5521 gaatggcgcg ccttccgccg ttccctgtat ttcgatgatt ttccccgatg cggtcatgac
     5581 gatgttcaca tcactgtcgc agccggagtc ttcgggataa tccaaatcca aaagcggcac
     5641 gccgttcact acgcccgctg atacggcggc aacagcttcg aggatggggt tttcactcaa
     5701 aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg caccggtaat
     5761 cgaagccgta cgcgtgccgc cgtccgcctg aatcacatcg caatcaatca ggatttgtcg
     5821 ttcgccgagt ttttccatat ccacgaccgc gcgcagcgaa cgcccgatca agcgttggat
     5881 ttcttgtgtg cgtcccgact gtttgcccgc cgaagcttcg cggcgcatac gcaaagcggt
     5941 tgaggcaggc agcataccgt attccgccgt tacccagcct tggtttttgc cgtgcagaaa
     6001 cggtggggca ttttcgtcca cggaagcggt acaaatcact ttggtattac cgcattcaat
     6061 aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat cgcgcaggct
     6121 gtcggcggcg cgtgaggtgc ggatataatc aggcatactg ccctcccgtt aaaaacagat
     6181 aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg aaaatttcta
     6241 cgcctcttcg gctttgctac ggataatcaa aagcggcagg tggctttggc gcattaccgt
     6301 ttcggcgaaa ctgcccatca aaaggtgcat cagcccggtg cgcccgtgcg tgcccaacac
     6361 cagcaggccg gcaccgtttt catcggcata atcgaccaaa tcctgcgcca tttcgcgcgc
     6421 acccttattg gcaaccagca ggtgtttgac ggtatttccc acacccagtt cccgggcggt
     6481 gcgctcggcg gcatccaaaa cctcgttgcc ttgcgcgacg gcggcggcct cgtaactttc
     6541 gtgttgcaaa aattcggggg cgagcgccat atattcggca gggttggcaa cgtgtaccaa
     6601 agtcagacgc gcaccgttga ccccggcaag ctcggcggcg tgtttcaggg cattgatgga
     6661 cgtttcactg ccgtcaacgg caacgaccag atgtttgtac atatcgtatt ctccttttgc
     6721 accgtctcac ggtgccgtct cttcaggttt gcgcaaaggc agtctgcgcc gtttcattat
     6781 agacccgccg tcgggcttta tacaacagcc gaacagcctg actgctttcc agtataatat
     6841 gccgctttcg tgcagtcagg catttttttg ccggctttcg ttcacttttt gatttgacgc
     6901 aatcttgcag gattcgacca tgtccgacaa cgctttgacc tcttcgcgac gcttcggcgg
     6961 catcgccaga ctctacggag actctgcctt gacgcacttt tcacaggcac acgtctgcgt
     7021 ggtcggcgtg ggcggggtcg gctcgtgggc ggtcgaggct ttggcgcgca cgggcatcgg
     7081 gcgtttgact ttgattgatt tggacaacgt tgccgaatcg aatgtcaacc gccagctgca
     7141 cgccctgacc ggcgacttcg gcaaagcaaa agttaccgcc ttgcgcgaac gtattacaca
     7201 aatcaatccg caatgcgaag tatttgaaat tgaagatttc gttaccgaag acaatttgcc
     7261 ggaatacttc ggaaaaggtt ttgatttcgt catcgacgcg atcgaccaag tgcgcgtcaa
     7321 agcggcaatg gcggcttatt ttgtggaacg caaacaaccg tttgtcctca gcggcggcgc
     7381 gggcggacag aaaaatcccg ccctgattca aaccgccgat ttaagccgcg taacccacga
     7441 cccgctgctt gccaacctgc gctacacctt gcggaaacgc tacggcttca gccgcgatac
     7501 taaggaaaaa atgcgcgtgc cgtgcgtgta ttcgaccgaa aatatcatgc cgccgcaatc
     7561 gggcgcagcg tgttctgccg atgccgcgcc gcagggcttg tcatgcgccg gctatggcgc
     7621 aagtatgctc gttaccgctt cgttcggact gtattgcgca caggcggcgg tggaacacat
     7681 cgcgggcaaa aaataagcaa tgccgtctga aacaggattc agacggcatt tgaacaaacg
     7741 cggcaatcaa gctgtcggtt cagaaaaacc aacatcggca cgcctaaaac cgtattcaca
     7801 atctactcct tttcaaaata tttgcattta aaagccgtta taatgccgtc cgaacatctg
     7861 cccgaccaca ttatgcgtga atgccggcag attgttttct tttgtaaact tatattaaaa
     7921 ttcacttacc aattcacgcc atgccgccca tccctgccct atccgcacca tccgagcaca
     7981 ctgtcgcatg ggtattcggc caacccgtta ccgatttgcc gcaggatttg tttatcccgc
     8041 ccgacgcgct gaaagtcata ttgggcagtt ttcaaggccc tttggatctg ctgctctacc
     8101 tgattcgcaa acagaatatc gacgtactgg atattccgat ggtggaaatt accgggcagt
     8161 atctgcacta tattgcccaa atggaagcct atcagtttga tttggcggcg gaatatcttt
     8221 tgatggcggc aatgctgatt gaaatcaaat cgcgcctgct gctgccgcgt accgaagccg
     8281 tcgaagacga agaggccgac ccgcgtgccg agttggtgcg ccgtctgctt gcctacgagc
     8341 aaatgaaact ggcggcgcag ggtttggacg cgctgccgcg tgcgggacgg gatttcgcgt
     8401 gggcttacct gccgctggaa attgcagccg agacgaagct gcccgaggtt tacatcgccg
     8461 atttgatgca ggcatggttg ggcattcttt ctcgggcaaa acatacgcgc agccacgaag
     8521 taatccaaga aaccctttcc gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg
     8581 aacacgggat atgcaggttt cacgacctgt tcaatcccga acagggcgcg gcttacgtga
     8641 tcgtcaactt catcgccctg ttggagcttg ccaaagaagg attggtcgga atcgtacagg
     8701 aagacggttt cggagaaatc cgaatcagcc tcaatcatga gggggcgcat tcagacggca
     8761 ttttcggcac acggggcggg cgcgatgtgt tctaatacgc cccaagccgc caccagaaat
     8821 cgggagacac gccatatgac cggcatcata cattcgctgc tcgacactga cctctacaaa
     8881 ttcactatgc tgcaagtagt cctgcaccag tttccgcaga cgcacagcct ttacgaattc
     8941 cgctgccgca acgtctcgac cgtctatccg cttgccgaca tcagggaaga cttggaagcc
     9001 gaactcgacg cgctctgccg gctgcgcttc acccacgacg aactcggcta tctgcgctcc
     9061 ctgcgtttca tcaaaagcga ctttgtcgat tatctcgaac tcttccagct ccaacgccgc
     9121 tttgtcgaag ccggcacaga cgataaaggc cgtctgaaca tccgtatcga aggcccgatg
     9181 atacaggcga tgttttttga aatcttcatc ctcgccattg tcaacgaact ttacttccgc
     9241 cggctggaaa cccctgccgt tatcgaagaa ggcgaacgcc ggcttcaagc caaagccgcg
     9301 cgcctgaaag aaatcgccgc cgcacagaat ccggacgaac cgcccttcct gatttccgac
     9361 ttcggcacgc gccgccgcta caagctcgcg tggcaggaac acgtcatccg caccctgctc
     9421 gaagccgccc caagcatcgt gcgcggcaca agcaacgttt acctcgccaa aaaactcggc
     9481 atcaccccca tcggcaccat ggcgcacgaa ttcctccaag ccttccaagc cctcgacgtg
     9541 cgcctgcgga atttccaaaa ggccgcgctc gaaagctggg tgcacgaata ccggggcgat
     9601 ttgggcgttg ccctgaccga cgtggtcggt atggatgcct tcctgcgcga tttcgacctc
     9661 tatttcgcca aactctttga cgggctgcgc cacgacagcg gcgaccctta cgtttggggc
     9721 gacaaagcct acgcccacta tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc
     9781 ttctccgacg ggctggacat cgaacgctct tgggcattgc accaatattt caaaggccgc
     9841 ttcaaaaccg gcttcggcat cggcaccaac ctcaccaacg atatggggca tacgcccttg
     9901 aatatcgtgt tgaaactggt cgaatgcaac gggcagtccg tcgccaagct ctccgactcg
     9961 ccgggcaaaa ccatgaccaa caacagcacc ttcctcgcct acctgcgcca agtgttcggc
    10021 atacccgaac ctagaacgcc gtaaaccggc agaaaaagcg tacaattcct gtttccgccg
    10081 cataaaatct tttaaaatac cgcctgattt gaatttaacc gaaagaccga acttcatgaa
    10141 cctacatcaa accgtcgaac acgaagccgc cgccgccttt gccgccgcag gcatcgccgg
    10201 cagccccgtt gtcttgcagc cgaccaaaaa tgccgagcac ggcgatttcc aaatcaacgg
    10261 cgtgatgggt gcggcgaaaa aagccaaaca aaatccgcgc gaactggcgc aaaaggtcgc
    10321 cgacgcattg gcgggcaacg ccgtcatcga aagcgcggaa gtggccggac ccggctttat
    10381 caacctgcgc ctgcgccacg aatttctcgc tcaaaacatt catgcggctt tgaacgatgc
    10441 gcgtttcggc atagcgcaaa ccgacaaacc gcaaaccgtc gtcatcgact attcctcgcc
    10501 caatctggcg aaggaaatgc acgtcggcca tctgcgttcc agcatcatcg gcgacagtat
    10561 ttcgcgcgtg ttggaattta cgggcaacac cgttatccgt caaaaccacg tcggcgactg
    10621 gggtacgcag ttcggcatgt tggtcgctta tttggtcgag cagcaaaaag acaatgccgc
    10681 gttcgagctg gcggatttgg agcagtttta ccgcgccgcc aaagtgcgct ttgacgaaga
    10741 ccctgccttt gccgacaccg cacgcgaata cgttgtgaag ctgcaaggcg gcgatgaaac
    10801 cgtgttggcg ttgtggaaac agtttgtcga tatttcgctc tcgcacgccc aagccgttta
    10861 cgacacgctg ggcttgaagc tgcgccccga agacgtggcg ggcgaatcga aatacaacga
    10921 cgatttgcag cccgtagccg atgatttggt tcaaaaaggc ctggcggttg aggacgacgg
    10981 cgcgaaagtc gtgttcttgg acgagtttaa aaacaaagaa ggcgaacccg ccgcatttat
    11041 cgtgcaaaaa caaggcggcg gcttccttta tgcctccacc gacttggcgt gcctgcgcta
    11101 ccgcataggc cgtctgaaag ccggccgcct gctgtacgtc gtcgaccacc gccaagccct
    11161 gcacttcgag caactcttta ccacttcccg caaagcaggc tatctgcctg aaaatgcaaa
    11221 agccgagttt atcggcttcg gcaccatgat gggcaaagac ggcaaaccgt tcaaaacgcg
    11281 cagcggcgac accgtaaaac tggttgacct gctgaccgaa gccgtcgagc gcgccaccgc
    11341 tttggtgaaa gaaaaaaatc ccgaattggg cgcggatgaa gccgccaaaa tcggcaagac
    11401 cgtcggcatc ggcgcagtca aatacgccga tttaagcaaa aaccgcacca gcgattatgt
    11461 gttcgactgg gacgccatgc tctcatttga aggcaacacc gccccctacc tgcaatacgc
    11521 ctacacccgc gtgcaaagcg tgttccgcaa agccggagaa tgggacgcaa ccgcaccgac
    11581 cgttttgacc gaaccactgg aaaaacagct tgccgccgag cttctgaaat ttgaaaacgt
    11641 gctgcaaagc gtggcggaca ccgcgtatcc gcactacctc gccgcctacc tttaccaagc
    11701 cgccaccctg ttcagccgct tctacgaagc ttgcccgatt ttgaaagccg aaggcgcaag
    11761 ccgcaacagc cgcctgcaac tggcaaaact caccggcaac acgctgaaac aaggcttgga
    11821 tttgctgggc atcgatgtgt tggacgtaat gtaaaaccgc accgcccgat tgcggacaac
    11881 agccctgcaa tcaaccggcg gaccatcctc atccgaatct gaaaaagcgg cgcgatacac
    11941 cgtatccgcc gcccctccca aaatgcgaaa cagacaaacg ccaagcaagc aagcaagcaa
    12001 gcaagcaagc aagcaaaaaa ttataccccc cttcctgccg acgcacgcac tttccgcgcg
    12061 gcgtattccc cttttcccgc ccctcaaacc cgccttttct tcaggcaggg tttcagcccg
    12121 cctcctttcc ctgttttcct tccctcaaca cgcgtgctcg ccccctgccg caccgtgccg
    12181 cactttcgcg cccggacggc atcgttccgc catccggttc tctgttttaa atacccctgt
    12241 ttcagaaaaa aatgcagatg tttcaacaca caggacgaca tataaagcac cgccctatgt
    12301 gtcgtcctga tttggaaggg gttgcgcccc ttcctaaata tagtctgatc ctgccgcccc
    12361 gaaggacaga tgtccgagtg gcggggtttc aaccgaaaag gaaatacgat gaatattcac
    12421 gccctgctct ccaaacaatg gacgctgccg ccattcctgc cgaaacggct gctgctgtcc
    12481 ctgctgatac tgctggcccc caatgcggtg ttttgggttt tggcactgct gaccgccacc
    12541 gcccgcccga ttgtcaattt ggactacctt cccgccgcgc tgctgatcgc cctgccttgg
    12601 cgtttcgtca aaattgccgg cgtattggcg ttttggccgg cggttttgtt tgacgggctg
    12661 atgatggtga tccaactctt cccttttatg gacctcatcg gcgccatcaa cctcgtcccc
    12721 ttcatcctga ccgcccccgc cccttatcag ataatgaccg ggctgttgct gctgtatatg
    12781 ctggcgatgc cgtttgtgtt gcaaaaagcc gccgtcaaaa ccgacttccg acacattgcc
    12841 gtctgtgccg ccgttgtggc ggcagccggc tatttcaccg gccatttgag ttactacgac
    12901 cgggggcgga tggccaatat cttcggcgca aacaacttct attacgccaa aagtcaggcg
    12961 atgctctaca ccgtcagcca gaatgccgac tttattaccg ccggcctggt cgaccccgtc
    13021 ttcctcccct tgggcaatca gcagcgtgcc gccacgcggc tgagtgagcc gaaatctcaa
    13081 aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg gcaatcccga gcttcaaaac
    13141 gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga aagcggcagt
    13201 tttcccttca tcggcgcgac ggtcgaaggc gaaatgcgcg aattgtgcgc ctacggcggt
    13261 ttgcgcgggt tcgcactgcg ccgcgcaccc gacgaaaaat ttgcccgctg cctccccaac
    13321 cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg gcgcgggtag ttcgctttac
    13381 gaccgcttca gctggtatcc gagggcgggc tttcaaaaaa tcaaaaccgc cgaaaacctg
    13441 atcggtaaaa aaacctgcgc cattttcggc ggcgtgtgcg acagcgagct gttcggcgaa
    13501 gtgtcggcat ttttcaaaaa acacgacaag ggactgtttt actggatgac gctgaccagc
    13561 cacgccgact atcccgaatc cgacattttc aaccacaggc tcaaatgcac cgaatacggc
    13621 ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc acacccaatt cttcgaccaa
    13681 ctggcggatt tgatccgacg ccccgaaatg aaaggcacgg aagtcatcat cgtcggcgac
    13741 catccgccgc ccgtcggcaa cctcaatgaa accttccgct acctcaaaca gggacacgtc
    13801 gcctggctgc acttcaaaat caaataacaa caatgccgtc tgaacgcacc aacagccttc
    13861 agacggcatt ttgcagacag accgacctcc tcaagcccac ttttttcatc atctccgata
    13921 aattgctttg taagaatcca aaaatccaaa atcacaggaa gttatcaaaa aaacagaaac
    13981 catccgccgt cgttcccgcg aaagcgggaa tccagacccg gaaaccgcag aaatttatcg
    14041 gaaaaaccga aactcgaaaa caacggacag aattcccgcc tgcgcaggaa tgacggctca
    14101 tacatcaccc aaaaaaaatt gaaaccaaac agctgggatt ccctcttgcg tgaggctgac
    14161 agatgccgtc tgaaagactt tcagacggca tagggcgttt tctctttgaa tttaaatttt
    14221 gccacaaaaa agccgcccca aatgaatacc cgggcagctt tttgttgata tgactccaat
    14281 cagcggtgtt gcggattgta acgtttttcc aaacgcagga atatccagcc taagaaagtc
    14341 gtcatcaaca gataaatcag ggcggcggtg taaagcggtt cttcataaac cgaataccgg
    14401 cccgtaatcg tattctgaac atacgccaac tccgccacag caatgaccga cagcagcgag
    14461 ctgtctttca agagcgtgat gaactcgctc gccaaaggcg gcagcatacg gcgcaatgcc
    14521 tgcggcagaa tcacatagcg catcgcctgc ggataggtca gtcccaaaga acacgccgcc
    14581 tccatctgtc ctttgtctat agactggatg cccgcgcgga aaatctcaca gatatacgcc
    14641 cccgagttgg cgatcagtgc caaagaaccg gcaatcagcg gcccgtatcc gcgacgcagc
    14701 gcgattgccg cctcgccgct gaccaaaatg ccgtctgaag gatggacgaa aaacggaaac
    14761 cacacatacg cccaaatcac aatctgcaca aacagcggcg taccccggaa cagggtaacg
    14821 tacagcagcg aaaccttacg caacgcccac gccagcacgc gcatcggcgc accggctttt
    14881 tccaagtgaa tcaggcgcgc caacgccaac aacagaccca ataccgaacc gcccgccgtc
    14941 gccacgaccg tcagccccaa ggtcgtcagt gcgccgtaaa gaaacatcca gcggtattcg
    15001 taaataatgt caaaacgaaa atccataaac cgtccgtatc aaaaaccggc ggaactgccg
    15061 ccgttgcaaa ataatccgcc attttaccgt aaaaaccgcc gcctgaactt ttttatcgcg
    15121 gcagacggcg gttgcgcgtc tccgcaaaaa tgcagggcgc gcggttttca gacggcattt
    15181 gccgttcaag gccgtgcggt gtctttacca aatgcccaac cattcgccca cggaatccat
    15241 ccaatcctta ttgcccccgc cgctcctgcc tgcccggcgg tacgcccacg gcgcttgcgg
    15301 atttttagct ttccacaatc ctttgcgttc cctttccgcc tgaatttgag cgtcggcata
    15361 gtcggcaaaa tccgccttat cctgctgttc tttagcataa cttttataat gccacgccgc
    15421 cccgtcctgc acctgcatca ggttcaaatc ggttttgccg gcggatacct gcgccacttc
    15481 gcgctgatag cggtcggttt caaacacacg tacactgact ttcctaccct ccgccgccgc
    15541 gcgcaggttg tcgcgcgaac gtgtaccgta agcctgtttc atctccggtg cgtcgatata
    15601 cgccatccga attttatgtt tcgcgccgtc gccgtcgatg acgtgaaggg tatcgccgtc
    15661 atagactttg gacaccgtgc ctgtgtagct gtggccggat ttcgccgatg cccgtcggcg
    15721 aacgggcgcg tcggaaccca cgtcccctgc cgtgccgagt gcgtcgagta cggcaaccgc
    15781 cgtccgcacc gcctcactgt catatcccgt ataacccaac gcgcccaaaa gcgacagggc
    15841 gacgggaagc catttcatga tttttttaat ctgcatattt ttcaaatgcc gatgccgtct
    15901 gaacatctct gaaccggatt tcagacggca tcttaacgtc aggattaccc ttggcaggga
    15961 tagatgactt tcgcaccctc ttccgtcccc aaaatcaaca catcggcggc atcgcgggcg
    16021 aatatgccgt tttcgagtac gccggtgatt ttgttgattt catcttccat cgtcagcggc
    16081 tgatcgatat tcaagccgtg gacatcgacg atttggttgc cgtaaaacgt ggtgtagccg
    16141 atacgcagtt cgggctgtcc gcccatggca agcagtttgc gcgaaacaag ggagcgcgcg
    16201 ctttcgacga cttccacggg cagtgggaat ttgcccagcc gggaaacata tttgctttcg
    16261 tccgcaatgc agacgaattt ttctgatgcg ctggcgacga ttttttcgtt gaggtgcgcg
    16321 ccgccgccgc ccttaatcat ttgcagggcg tggttcactt cgtccgcacc gtcgatatag
    16381 accgccaaac cggacacttc gttcagggaa acgacgggaa tgtcgtaccg ggcaagcagt
    16441 tcgccggatt ttttggaggt tgaaaccgcg cctttgattt ttttaccgct tttgcccaag
    16501 gcttcgatga agaaattgat ggtcgaaccg gtaccgatgc cgatgtattc gttttcgggt
    16561 acgaattcga ctgttttttc ggcggcgatg cgcttgagtt cgtcttgtgt cgtcatattt
    16621 ttatcctttg ggaaaccgta tcaacaaaca gccgccatct taacattttt ttgcacgtcc
    16681 tgcccgccgc gttcaaatcc gtaccagcaa taccgccgcc tgcgcctcta tgccttccat
    16741 ccgcccgaga tagccgagtt tttcgttggt tttgcctttg atattgacgc acgaaatatc
    16801 gatacccaaa tcggcggcga tgttggcacg catttgcgga atgtgcggtg cgagtttggg
    16861 tttctgtgca atcacggtcg tatcgacatt gaccgcctgc caaccctgcg cctgaacgct
    16921 ttgatacgcc gcacgcaaaa ggacgcggct gtccgcatct ttgaactctg cggcggtgtc
    16981 gggaaaatgg ctgccgatgt cgcccaaacc tgccgcaccg agcagcgcgt cggtaacggc
    17041 gtgcagcagc gcgtcggcat cggaatgccc gagcagccct ttttcaaatg ggatttcaac
    17101 tccgccaagt atcagctttc tgccttcgat caattggtgg acatcgtagc cctgtccgat
    17161 acggatattc gtcatcgttt gtgttcctga tgttttgaat tgaatttcag acagcattga
    17221 gcagcagcct gacgatgtat gcgtcctgcg gctgcgtcag tttcaaattg cgcgcgtcgc
    17281 cctgtatcag tagcggacgc acacccagtt tttccacggc ggacgcttca tcggtaatgc
    17341 cgtccaagtt ttccgcagcc aatgcgcggt gcagcaaacc cgcttgaaaa agctgcggcg
    17401 tttgcgcctg ccaaagcccc gaacggtcga cagttgcact gatttgtccg ctttctgcgc
    17461 gcttgagcgt atcggcaacg ggaactgcca aaatcccgcc ttcggcggcg ttgcccgcct
    17521 gttctatcaa ccgcgccaga gcttcagacg gcaggcagca gcgggcggca tcgtgtacca
    17581 gaatattgtc ggtttccgcc gccaaaccgg tttccaacag ttttgccaca ccgttgcgga
    17641 cagtttcggc gcgggtctgt ccaccgtttt tccacacccg aacctgtgga aatgccgtct
    17701 gaaccttatc ggcaaacgtg tcttcgggcg agacgacaac gacggtcaaa tcgacggcct
    17761 catgccgttc aaaaatccca agtacatgtt ctaaaacggt tttgcttccg atttcgacat
    17821 attgcttggg tttgtccgca ccgaaacgca ccccgatgcc ggcggcggga atcagcgcga
    17881 tatttttgcg tttcatgcgt tttccccgcc gttttcagac ggcacggctt ccttacgcca
    17941 aatacaggct tcgcccaagc cgtcaagata ttcttcgtgc gccgccagtt cggcttcatc
    18001 cgccttaatc actttcagtt tggcaccgag tttcatttcg gcagatgcaa caggtttggc
    18061 tgtcgtttct tcctcctcgg tctcccccat caagtcgaat tgccgccgcg tcatcgccag
    18121 atagacttcg cccaaaagtt cgcagtcgat caatgcgccg tgcaggacgc gcttgctgcg
    18181 gtcgacggaa aaacggttgc acagggcatc cagactggct ttctgcccgg ggaacatttc
    18241 gcgcgccatc gccagggtat cggtaacggt gcagccgagt tcctcgacgg tcggcagccc
    18301 cacccggcgg aactccatat tgaggaagcc cacatcgaat ttggcattgt ggataatcag
    18361 ttccgcaccg cgcagaaaat cggcaatctg cctgccgacc tctgcaaacg gcggcgcgtt
    18421 tttctcttcc aaaacctgta tcgtcaagcc gtggacgcgt gccgcctctt cgggcatatc
    18481 gcgctcgggg tggacataga ggtgcaggtt tttgtcggtc atttggcggt tgaccatttc
    18541 cagaccggca aactcgacca gacggtcgcc gccgtcggca tacaggccgg tggtttcggt
    18601 atcgaggatg atttggcgtg tcatcatatc ggtgtctttc ttctgtcttc gtaaattgat
    18661 tattttttaa gcaatgtatt tttctgtttt catttcaatg cacaaaccca cttattcaca
    18721 gtgtgttcac aacattgggc aggcggattg tgtattttgg ggacaatttt ttcagacggc
    18781 attcaaggtt ttttcctgat tgccgccgcg cctaaaaacc gcctttcgcg cttaatcaaa
    18841 aataccgcca acggaatatt gcccaaggcg acaatcagat acagcaggga aatgctgtca
    18901 aacaaaaaca gcaataccgc gctcaaaacg gcggcggaaa ccataaagat gccgttaacg
    18961 atattgttgg cggcaacggc gcgggcgcgg aaagtctcgc tgctggcggt ttgcagccag
    19021 gtatagagcg gaacggagaa aaatccgccg aaaaagccga tcagcgtcat caccgccatc
    19081 acggggtatg cccatccttg cgataaaaac caaaaaatgc cgttcagccc ttcaaaacgg
    19141 tgtccgtgcg tcagccacac caaaaccagg ccgcaaaccg tcaaacccaa cgcaccaacc
    19201 gttacccaag ccaacatcag ccgttccctg ccgaacttgg cacacagtac cgaaccggcg
    19261 gcaataccga tggaaaacaa agcaagcatc aggttaaaaa cattatcgtt gccgcccaaa
    19321 tggatttggg taaaggtcgg cagttgcgtg gtataaaccg cgccgacaaa ccaaaaccac
    19381 gagatgccga taatggcggt aaaaacggga ttgtgccgca ccgtttcacg cagcagggat
    19441 tttgtaccac ggacaatatt ccactcgatt tgggtatcgg cagccttggc gggtacggac
    19501 ggcataaaca ggctgccggc cgttcctccg acggcgacca gcaaaaccag tatcccgaca
    19561 atataaggcg gcgcgccggc aaccgccgtt cccaaaatct gaccaaacag gatggcgaca
    19621 aacgtaccgg attcaatcag gctgttgccc atcatcagct cgttgtcgtc gagataatcg
    19681 ggcagaatgg catatttaag cgggccgaac agcgtcgatt gcgcgcccat gcaaaacagg
    19741 cagagcaaga gcagcggggc tgaccggata taaaacccgt atgccgccac cgccataatg
    19801 atgatttcca gcaccttgac ccaacgcgcc aaaacggcct tgtcgaattt gttacccaac
    19861 tgccccgaca gcgcggaaaa caggaaatac ggcaaaataa acagcagtgc gcccaagttc
    19921 aacatctgtc cggcaggcag aacgccgttt tgccccaaac cgtaaaaccc gatcatcaca
    19981 aacagcgcgg ttttgaacac attgtcattg aacgcgccga gaaactgcgt agcgaaaaga
    20041 ggcccgaaac ggcggctttt aaccagtccc aaaccgcctt ttttagcgtc catcgttttc
    20101 cttctctttt tcaatcagtt tacttgtcga atcatcatcc atcaaaatgc ggtgcgccgg
    20161 tccttccaaa tcgtcaaact gtccgttttt gcccgaccac cagaaaaacc agccgatgac
    20221 aaatgccaaa ataatgctga tgggcaccag aataaacata ctttccatca catattccct
    20281 atcaaatcgt tcaaaacaaa agtctgccct gacacggtca gatattcgtt acgcaaagtt
    20341 ccgacggggg cttcgtcaaa aaacagctcg atacggtctt tgaccacgcg ccaatattgg
    20401 ggaatttccg tctgaccgaa cggcgacagg acatggttgc ccgtcccatc ttccaattta
    20461 acggcaaaac gcccgctctg cagccgtgct tccgattcgt cgtcggcaag aaggatgaaa
    20521 aagcctatat gccgtcccga ttggtcatga atactgaaat aatgcataaa ttccccaccc
    20581 gccttttttc agacggcatc aactaaaaat agggcgaatg taccagtttg gacgggaaaa
    20641 atgcaaagaa attctccctc ccccagccga aaacaccggc aaaccgcata tccccctttt
    20701 ttccgtcaaa atacccgact tccgccattt tcacgcaaac gcccgattaa gccaagcaat
    20761 tgcaaagatt ttttgctaga atagcctgct tctt
//
LOCUS       DAXFHF010000039        19927 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXFHF010000039 DAXFHF010000000
VERSION     DAXFHF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19927
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.039"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            50..322
                     /locus_tag="ACFIRM_001288"
     CDS             50..322
                     /locus_tag="ACFIRM_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028911.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="ACFIRM_001289"
     CDS             304..456
                     /locus_tag="ACFIRM_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028912.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="ACFIRM_001290"
     CDS             443..988
                     /locus_tag="ACFIRM_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC3028913.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLNNEVDNLSDMKRKPFAW"
     gene            1392..1793
                     /locus_tag="ACFIRM_001291"
     CDS             1392..1793
                     /locus_tag="ACFIRM_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028914.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            2105..3238
                     /locus_tag="ACFIRM_001292"
     CDS             2105..3238
                     /locus_tag="ACFIRM_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028915.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAQWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            3245..3565
                     /locus_tag="ACFIRM_001293"
     CDS             3245..3565
                     /locus_tag="ACFIRM_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028916.1"
                     /translation="MPSETGFQTALESQTLFAMPYMPNPVRYKLKTTIQKTEIHIIKQ
                     TNKLTYCAILRAVPYFPVLYMSGRHPHKRRDTDKEKSLGIKNILINQYKSNKQFIIFF
                     SVGV"
     gene            complement(3741..4016)
                     /locus_tag="ACFIRM_001294"
     CDS             complement(3741..4016)
                     /locus_tag="ACFIRM_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3028917.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(4013..5296)
                     /locus_tag="ACFIRM_001295"
     CDS             complement(4013..5296)
                     /locus_tag="ACFIRM_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3028918.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5445..6863)
                     /gene="glnA"
                     /locus_tag="ACFIRM_001296"
     CDS             complement(5445..6863)
                     /gene="glnA"
                     /locus_tag="ACFIRM_001296"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC3028919.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            7174..7983
                     /gene="aroE"
                     /locus_tag="ACFIRM_001297"
     CDS             7174..7983
                     /gene="aroE"
                     /locus_tag="ACFIRM_001297"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC3028920.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            7986..8687
                     /gene="mtgA"
                     /locus_tag="ACFIRM_001298"
     CDS             7986..8687
                     /gene="mtgA"
                     /locus_tag="ACFIRM_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC3028921.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(8961..9036)
                     /locus_tag="ACFIRM_001299"
     tRNA            complement(8961..9036)
                     /locus_tag="ACFIRM_001299"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9001..9003),aa:Phe,seq:gaa)
     gene            complement(9227..9961)
                     /gene="lptB"
                     /locus_tag="ACFIRM_001300"
     CDS             complement(9227..9961)
                     /gene="lptB"
                     /locus_tag="ACFIRM_001300"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC3028922.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(10032..10562)
                     /gene="lptA"
                     /locus_tag="ACFIRM_001301"
     CDS             complement(10032..10562)
                     /gene="lptA"
                     /locus_tag="ACFIRM_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC3028923.1"
                     /translation="MIQNICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(10543..11124)
                     /gene="lptC"
                     /locus_tag="ACFIRM_001302"
     CDS             complement(10543..11124)
                     /gene="lptC"
                     /locus_tag="ACFIRM_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC3028924.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKHM"
     gene            complement(11121..11657)
                     /locus_tag="ACFIRM_001303"
     CDS             complement(11121..11657)
                     /locus_tag="ACFIRM_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC3028925.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(11723..12697)
                     /locus_tag="ACFIRM_001304"
     CDS             complement(11723..12697)
                     /locus_tag="ACFIRM_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC3028926.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            12794..13849
                     /gene="tal"
                     /locus_tag="ACFIRM_001305"
     CDS             12794..13849
                     /gene="tal"
                     /locus_tag="ACFIRM_001305"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC3028927.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEALARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            13861..13995
                     /locus_tag="ACFIRM_001306"
     CDS             13861..13995
                     /locus_tag="ACFIRM_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028928.1"
                     /translation="MPSERLSDGIARLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            14000..14290
                     /locus_tag="ACFIRM_001307"
     CDS             14000..14290
                     /locus_tag="ACFIRM_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028929.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            14307..15194
                     /gene="gluQRS"
                     /locus_tag="ACFIRM_001308"
     CDS             14307..15194
                     /gene="gluQRS"
                     /locus_tag="ACFIRM_001308"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC3028930.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(15264..16280)
                     /gene="dusA"
                     /locus_tag="ACFIRM_001309"
     CDS             complement(15264..16280)
                     /gene="dusA"
                     /locus_tag="ACFIRM_001309"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC3028931.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            16400..17554
                     /locus_tag="ACFIRM_001310"
     CDS             16400..17554
                     /locus_tag="ACFIRM_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3028932.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLGIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(17604..17870)
                     /locus_tag="ACFIRM_001311"
     CDS             complement(17604..17870)
                     /locus_tag="ACFIRM_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC3028933.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(17978..18925)
                     /locus_tag="ACFIRM_001312"
     CDS             complement(17978..18925)
                     /locus_tag="ACFIRM_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3028934.1"
                     /translation="MTFQGHNNRKKAGGYAEYITGGSLRRLVAAKVRRYCGEHPGVFD
                     GAAGSGQLEQYIEPSDFRAVEIQAEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVM
                     NPPFSIKLKDLSEDEKSRIAREYPWKKSGVADEIFVLKGLENARRFGFFILFPGIAYR
                     KSEQRFREIIGNRLAELNRIQNAFEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLL
                     AADTWQIEPDLWQTVQEPAPPKEKEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGW
                     PHAEFDGFCDRLCNLIQAEKYGKKHYFPCSLPLFGGAAG"
     gene            complement(18897..19127)
                     /locus_tag="ACFIRM_001313"
     CDS             complement(18897..19127)
                     /locus_tag="ACFIRM_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028935.1"
                     /translation="MRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVFQRGEDGK
                     PQRVSRIRKRSATPGQAKERTKTHDVSRTQQP"
     gene            complement(19124..>19927)
                     /locus_tag="ACFIRM_001314"
     CDS             complement(19124..>19927)
                     /locus_tag="ACFIRM_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028936.1"
                     /translation="GAKVSEDEALTCGIMMRLSLQDMRYACNQELINFAEHIVKQVQR
                     LGLYCNTDDPENEESVLFACREASQAVAKWTKDFDNLSPNQRQLVLRPLSNLFAAYEE
                     FLKDAPARLIAEVSAYSLAVRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKM
                     PYAEFTGRILHAANLLYDVGIQADKELSAMYGKPLNPVRPRRISDVRRPMMKMLVADK
                     GGALVRAVKDSEDVIRHCDNGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT         4669 a         5179 c         5299 g         4780 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgcggtaac aattattcgg gattgaagaa tgatgagtgt
       61 aaaagaatta ttcaaagtca tcttagatga aaataaagat tttcctatta gaacgattca
      121 tcgtaccccg atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagacggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctgaataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg cttggtaagg gctttcttaa atttgattaa aggtcgtctg
     1021 aaattaagct tgtttttcag atggtctttc cttcatttga agcaggatat tgagaactga
     1081 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1141 ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc
     1201 aatgccgccg aaaccgtcga agccgccttc aacattgcca ccgccaaagc cgcgaagttg
     1261 gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat gtgaggatag
     1321 acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa acttagatat
     1381 ggaaaacgat tgtgaagatt aaaccattac aattttctaa caataatcac agattttatg
     1441 tggacaaaat ttgtgttacc gaacaagatg tgaacatttt ggcatccgac cgaaattatg
     1501 aattaaatat tgaaatattt attgacaata taattcattt tcaaataacg gatgaatctt
     1561 ataaagtaaa attttcagaa tatttattcg aaaataaaac agaaaataat tcggatggga
     1621 atcctactgc aaattatttt ttcgagataa tagatgacag ttatatggac tggttgaaag
     1681 aagaaagttt tggttttttt gaaaagaaat attataaggc ttatattttc ttttttagcg
     1741 attctgtaat agaagttatc agctcgacag aacctgtatt ttattcaaaa taacaaatta
     1801 tcaaacaaag ctctgattaa aaacccaaca atcaaaatgc catctgaagc ggtattcggc
     1861 tttcagacgg cattttctcc gattagtaca ggatgcaggg gcttgccggc ttgattttgc
     1921 cgaagttgtt ggcacgcaat ttggaggggg cgacaaccta tgaaaaataa atcgttaagg
     1981 atatttttag ttttacccaa tttgtttccg aaaaagtacg gcagcaaccg tagattagat
     2041 taaaaatcaa acaatcaaag cccgcctgat ataatcgata tatcctgtta aacgaaaaat
     2101 aaatatgttt aaaaaattca aaccggtatt gttgtcattt tttgcacttg tatttgcctt
     2161 ttggctggga acgggcattg cctatgagat taatccgcgt tggtttttga gcgatacggc
     2221 aactgaaata cctgaaaatc cgaatacttt tgtggcgaaa cttgcccgcc tgttccgaaa
     2281 tgccgacagg gcggttgtca tcgtgaagga atcgatgagg acggaggaaa gccttgccga
     2341 aactgtggat gacggtccgt tgcagtcgga gaaggattat ctcgcgctcg ctatccggct
     2401 cagtcgtttg aaagaaaagg cgcaatggtt tcacgtaacg gagcaggaac atggggaaga
     2461 ggtctggctg gattactata tcggcgaggg cggtttggtt gcggtttcgc ttttgcaacg
     2521 atcgccggaa gcgtttgtta atgccgaata tctgtatcgg aacgatcgtc cgttttctgt
     2581 aaatgtgtac ggcggaacgg ctcacgggga aaattatgaa acgacaggag aatatcgggt
     2641 tgtttggcaa ccggacggtt cggtatttga tgcgtcgggg cgcgggaaaa tcggggaaga
     2701 tgtttatgag cattgcctcg ggtgttatca gatggcccag gtatatttgg cgaaataccg
     2761 ggatgtcgcg aatgacgagc agaaggtttg ggacttccgc gaagagagca accggattgc
     2821 gtcggactcg cgcgattatg tgttttatca gaatatgcgg gaattgatgc cccgggggat
     2881 gaaggcgaac agtcttgtgg tcggctatga tgcggacggt ctgccgcaaa aagtctattg
     2941 gagtttcgac aatggaaaaa aacgccagag tttcgaatat tatttgaaaa acggaaatct
     3001 ttttattgca caatcttcga cggtagcatt gaaagcggat ggcgtaacgg cggatatgca
     3061 gacctatcat gcgcaacaga cgtggtattt ggatggcggg cggattatcc gcgaagagaa
     3121 acagggagac agactgcctg attttccttt gaacttggaa gatttggaaa aagaggtgag
     3181 ccgttatgca gaggctgcgg cgagacgttc gggcggcagg cgcgaccttt ctcactgaac
     3241 gcaaatgccg tctgaaaccg gttttcagac ggcattggag agtcaaacgc tttttgcaat
     3301 gccgtatatg ccgaacccgg ttcgctacaa attgaaaacc acaatccaaa aaacggaaat
     3361 acacattatc aaacaaacaa ataagttgac atattgtgcg atattgaggg ctgtaccgta
     3421 ttttcccgtt ctttatatga gcggcaggca tcctcataaa agaagggata ccgataagga
     3481 aaaatccctc ggtataaaaa atatactgat taatcaatac aaatccaaca aacagttcat
     3541 cattttcttt tctgttggcg tataaaaacc gctcggaaaa aaataaaata tcaaaacggc
     3601 aaaataaaaa tactgatata tgccttcact ttcatagggc gacgctctat gtgccgtcct
     3661 gtgcgttgaa acatcggttt tccttgccgc ctgtcccgct tttccctgat tgctgccaac
     3721 tgccgtccga acctgaaccg tcaggtttca gacggcattt tttctgcctg ctcccacaat
     3781 tgccgttttt gtgcggcggt cagttttttg acggcgattt cctgtttgag cgcagtgcct
     3841 ttatccatgc cgcctgcaac gatacgcatc gccaccggtt tgaatacgcg ggtatatttc
     3901 gcgccttttc cggctgtgtg gatggcaagc cgctgttgcg gattcgggct gataccgcaa
     3961 tagaacgcgc tgttttcaca caatatcagg taaacgctcc agtttgacgc gttcatctgc
     4021 ctgcatcctg ggttttttcc ccgttccaag gcgcaacttt cagcagcagc agcatacccg
     4081 gcagggcaag tatgaaacac agccggaaaa acggtacata acccatccat tcgatcagat
     4141 agcctgcaaa ggaattgatg accgtgcgcg gcacggcgga caggctggta aacagcgcaa
     4201 gctgcgttgc cgtaaacgcg ggattggtct cgcgcgccat atacgatacg aacgccgccg
     4261 tccccaaccc cacgccgacc gcttccgcgc cgataactgc cgccagcatc agcctctcgc
     4321 ctgtgccgac cgtgtcgaaa tgtccgaacc ctgccagcca tacaaacccc aaaaccgtta
     4381 ttgcctgcac cgcgccgaat agccacaagg ctttgtttac gccgattttc agcatccaca
     4441 cgccgcccaa gatacctgcc gccactgccg gccacagtcc tgcatttttg gcaatcaaac
     4501 cgatgtcggt cttgctgaaa cccatatcca gataaaacgg cgttgccaac gcggttgcca
     4561 tactgtcgcc gagtttgtaa aggaagataa acagcagcac gcacaccgcc gaagcgatgc
     4621 ccttgcgcgt aaaaaattct ttaaacggct ctaccacggt ctgcttcaac gttttaggaa
     4681 cggaaggcgg caacacgggt tcgtgcgcga gaaacagcgt catcagaagg ccgggcagca
     4741 taaataatga agtgataaca aatacttctg accacggcat cctgtctgcc aacaccaaac
     4801 tcaatgaacc gggaatcagg gcggcaaccc ggtaggcgtt cacatgaacc gagttgccca
     4861 aacccaattc ttcgtccgac aaaatctcgc gcctgaacgc atccaataca atgtcctgac
     4921 tggcggaaaa aaaagcgaca agcaccgaca agccggcaat caacggcaga tgattccggg
     4981 ggtttaaaaa ggcatatgcc gccaaagccg ccagtaaccc tgcctgcgtc agcagcatcc
     5041 acccgcgccg ccgtcccaaa acgggcagcc tgaccgcgtc catcagcggc gaccacaaaa
     5101 atttccaagt aaacggcaga ccgatcaacg ccatcagccc gatgctcttc aaatccacct
     5161 gctcgctgcg caaccacgcc ggaatcaggt taatcagaaa gtacagcggc agccccgagg
     5221 caaaccccgt aaaaatacag acgagcatat tacgggaaaa gatttgcccg ataaaacctg
     5281 atttcgatgc agtcatcgtt cgtacagcca aaaaacaaaa gcctattgta gcaaatatcg
     5341 cccgcctgct gaaaaatgcc gccacgcgcc gccggtttgc cgttccaatg caaaatgccg
     5401 tctgaaactg tgttcagacg gcattgctac tgaaacccga cggtttacag gctgtaatac
     5461 atttcaaatt ccagcgggtg cggcgccata cggatgcggc ggacatcctc ctctttaaag
     5521 gcgatgtagc tgtcgatcca gtctttgctg aacacgccgc cgcgcagcag gaattcgtgg
     5581 tcggccttga gggcggcaag tgcttcttcc aaagaagcgc agacggtcgg gacgagcgcg
     5641 tcttcttccg gcggcaggtc gtagaggttt ttatcggcag ggtcgcccgg atggattttg
     5701 ttttgaatgc cgtccaaacc cgccatcagc agggcggcaa atgccaaata cgggttggcg
     5761 gtcgggtcgg ggaaacgcgc ttcgatgcgg cgcgccttgc tgctgttcac agacgggata
     5821 cggatggaag cggaacggtt tttggcggaa taggccaatt tggtcggtgc ttcaaagtgt
     5881 ggcacgaggc gtttgtagga gttggtggac ggattggtaa tcgcgttcag ggctttggcg
     5941 tgtttgatga tgccgccgat gtagtagagc gcggtatcgg acaaaccggc atagccgtcg
     6001 cctgcgaaca ggttttgacc gtctttccaa atggattggt ggacgtgcat accgctgccg
     6061 ttgtcgccca taatcggttt gggcataaag gtggcggttt tgccgaaatt gtgggcaacg
     6121 ttttggatga cgtatttcat atcttgggtt tggtcggcgc gtttgaccaa agtggcgaaa
     6181 cgggtgccga tttccatttg gctgccggta ccgacttcgc tgtggtggac ttcgacttcg
     6241 atgccgagtc cttccaaaat gttcaccatg gcggagcgca aatcttgacc gcagtcaatc
     6301 ggcgcgacgg gcgcgtagcc gcctttgacg gcggggcggt ggccggtgtt ttgaccgtcc
     6361 atatgcaggc cgcttgccca cgcgccgctt tcggacgtga tttcgtaacg ggttttgtgc
     6421 atgtcggttt caaattctac gccgtcgaag acgaagaatt cgggttcggg gccgaaatag
     6481 gcggtgtcgc cgataccgga agatttcaaa taggcttcgg cgcggcgtgc gatggagcgc
     6541 gggtcgcggt cgtaaccctg accgtcggca gggtcgatga cgtcgcaggt aatgacgacg
     6601 gtaacatcat cataaaaagg atcgacgaag gctgtggacg catcgggacg cagctgcata
     6661 tcggaagcct caatgccttt ccagccgccg atggacgagc cgtcaaacgc cggtccgttt
     6721 tcaaaccact cttcggggtc ttccaacacg atgcgcgcag gcacggtaaa gtggtgctgc
     6781 ttgcctttgg tatcggtgaa gcgcaaatcg acaaagcggg cttcgctttc ttcaatcaat
     6841 tttacggcgt ttttaatgga catcttcagc tcctgaaaaa gggaaatggc aaaatatgcg
     6901 gaacgcggcg gcttgacgca aagtcggcac gattttacca cgccgatgcc gtctgaaacc
     6961 ggttttgcgt caaattccaa gccggaaaat atagtttcaa acggcaagat tgtcaacaat
     7021 atgcttcggt ttgtctgttc aaaagcggaa tgcccgccct tttgtccgaa tgccgacaaa
     7081 acagatgccg tctgaaggcg gtttcagacg gcatcgttcc catcttcgcg ccgccgttat
     7141 aaaatgaccg ccccgccttt atggaatatg actatgcacg ccctcccccg ctacgccgtt
     7201 ttcggcaacc ccgccgccca cagcaaatcg ccgcagattc atcagcaatt tgcccttcag
     7261 gaaggcgttg acatcgaata cggacgcatt tgcgccgaca tcggcggttt cgcgcaggcg
     7321 gtttcgacat tttttgaaac aggcggttgc ggggcaaacg ttaccgtacc gttcaaacag
     7381 gaagcgtttc atctggcgga cgaacattcc gaccgcgctt tggctgccgg tgcggtcaat
     7441 acgctggtgt ggttggaaga cggcagaata cgcggcgaca ataccgacgg tatcggtttg
     7501 gccaacgaca tcacgcaggt caaaaacatt gccatcgagg gcaaaaccat cttgcttttg
     7561 ggcgcgggcg gcgcggtgcg cggcgtgatt ccggttttga aagaacaccg ccctgcccgt
     7621 atcgtcattg ccaaccgtac ccgcgccaaa gccgaggaat tggcgcggct tttcggcatt
     7681 gaagccgtcc cgatggcgga tgtgaacggc ggtttcgata tcatcatcaa cggcacgtcc
     7741 ggcggcttga gcggtcagct tcctgccgtc agccctaaaa ttttccgcga ctgccgcctt
     7801 gcctacgata tggtttacgg cgaagcggca aaaccgtttt tagattttgc acgacaatcg
     7861 ggtgcgaaaa aaactgccga cggactgggt atgctagtcg gtcaggcggc ggcttcctac
     7921 gccctctggc gcggtttcaa accggacatc cgccccgtca tcgaacacat gaaagccctg
     7981 taaccatgtt ccgcatcgtc aaatggctga ttgccctgcc cgtcggcatc tttatcttct
     8041 tcaatgccta tgtgtacggc aacatcatta cctaccgcgc cgtcgcgccc catcggactg
     8101 cctttatgtc gatgcggatg aagcagtttg aacaagaagg tcgcgatgtc gcactggatt
     8161 accgctggat gccctacaaa cgcatttcca ccaacctgaa aaaagccctg attgcttccg
     8221 aagatgtccg ttttgccgga cacggcggct tcgattgggg cggcattcaa aacgccatca
     8281 ggcgcaaccg gaacagcggc gaagtgaagg cgggcggatc gaccatcagc cagcagcttg
     8341 ccaaaaacct cttcctcaac gaaagccgca actatctgcg caaaggggaa gaggcggcca
     8401 ttacggcaat gatggaagct gttaccgaca aaaacaggat tttcgaactg tatttaaact
     8461 caatcgaatg gcactacggc gttttcggcg cggaagctgc gtcccggtat ttttataaaa
     8521 aaccggccgc agacctgacc aaacagcagg cggcgaaact gacggcactc gtccccgccc
     8581 cgctctacta cgctgaccat ccgaaaagca aacggctgcg caacaaaacc aatatcgtgc
     8641 tcagacgcat gggttcggca gaattacccg aaagcgatac ggactgattg tttcagatat
     8701 ggaaatgccg tctgaactgc ggttcggacg gcatatgttt tctggttctt atagtggatt
     8761 aaatttaaac cggtacggcg ttaccccgcc ttgccgtact atgttgtact gtctgcggct
     8821 tcgtcgcttt gtcctgattt ttgttaatcc actaacctgc ccgacagccg caaagaccgt
     8881 atcgtgcatg gcaggtattt gcggtgcggg actgctgccg aaaacggcag gcaaaaaata
     8941 accgcttata agcggttttt tggtggccag aggcggaatc gaaccgccga cacacggatt
     9001 ttcaatccgt tgctctacca actgagctat ctggcctgta tctgtctcgt taagaggtgc
     9061 gtattaaacc aaatttcggg acgacacgca agcggatttg tcgatatttt ttacccagcc
     9121 gactaagtcg atgttttaag tttaaattaa tttcacagaa aaatagtgca aatcggtttt
     9181 ttgccgttcg ttcggcgatg ttctgctttg cagacggcaa aacgcttcaa tatttgaagt
     9241 tctcgcccaa atagacggcg cgcacctgtt cgttgccgac caaatcgtcg ggcttgccgg
     9301 aagccagcac cgcaccgtct gaaatgatgt aggcgcggtc gcagatgctg agggtctcgc
     9361 ggacgttgtg gtcggtaatc aatacgccga tgccgcgcga tttgaggaaa ccgatgattt
     9421 tctggatgtc gatgacggcg atgggatcga cgccggcaaa aggttcgtcc aaaaggatga
     9481 aacgtggttt catggcgagg acacgggcga tttcgacgcg ccgccgttcg ccgccggaca
     9541 gcgacggcgc aggattgcgg cgcaagtgtc cgatattgag gtcggcgagc agtttttcga
     9601 tttctctgtc gatttgattt ttatctttgg ttctgatttc caagatggcg cggatgtttt
     9661 gttcgacggt cattttgcgg aatatcgagg cttcctgcgg caggtagccg acacccaggc
     9721 gggcgcgttc gtgtatgggc aggtggcgca attcccgccc gtccagcatc acgctgcctg
     9781 cgtcggcagc gatgagcccg actatcatgt agaagccggt ggttttgcct gcgccgttgg
     9841 gcccgagcag tccgatgact tcgccgcttt cgatctcaag ggagaaactt ttgacgactt
     9901 ggcgtttttt gaaacttttc tgcaggtttt ggacgacgag gcggctgttg tttgcgctca
     9961 taaatctctt ctcggcattt cggtcggcaa atgccgtctg aactgcgttt ccgtttcaga
    10021 cggcatcggg gttattcggt tttttgtgtg cttgaaggct ggatgacgac gctgaccctg
    10081 ccggttttgg aagcggattt cgcacccgat ttcgtgctgc cgttgatggt atagacttcg
    10141 gttttggtgt tgtaggtaat gaccgcacct tcggcaacgt cgccgccgcg ctgcactttg
    10201 gcattgccgg tcagaacgac ggtgcttcct gcggaggaat aggtaacgtt gtttgcctga
    10261 ccgcgcaccg tccctttgcc cccgtccaac gtttggctga agcggacggg cgaaccttcc
    10321 gccctcacgg attcgccgcc tttgccgccg cgtgtgacgt tgacgcgcga ggcggaaatg
    10381 ttgagcgtac cctgtctgat gatgacattg ccgctaaatg tggtactttg gttggcttga
    10441 tcgagcgaac cttggtcggc ttcgatttgg atgggccgtc tgctgtcgct ttgaagggca
    10501 aaagcgggag aagttgcaaa aattacaatt aaaacaaata gcttacatat gttttgtatc
    10561 ataaatcgcg gctttcactt tagatgagaa gttcaacatg cctgttttgt ggttgtaggt
    10621 cataccgccc gcctgaccgt gcgatgcacc atattggaaa ctgacaggcg tatcggtttg
    10681 ggcatattga gattcggtat cgacgtgcag tttttcggtt tcgactttac ccgcctgccg
    10741 cctgccgtcg gcggttttgg tcagcacaac gttgttttta aaaagaacct gtttgttttc
    10801 ggtatggtaa acggcttcat cgctgccgac ttcgtacaac agcctgcctt cttggaagaa
    10861 cacgagatgc ggcgaatcaa aatggatgtc gctgttttcg ggaaactgtt tcgcaccttt
    10921 cgcgctcaaa tgttctttca agtatccctg ttcgtcaaac cgccttccgt ccaagccgtc
    10981 cattgtgtat tgaggttcgt cgggattgag cctgacttcc tcgatttcga cttcgctgat
    11041 acggcccaac catgccgaca ggctgcccaa ggcaaccgcc aatatcaatg ggaacgcaat
    11101 tccgtaccgc catctcactt tcatttgatg tacccgttca aagccgcgcc caacgtacct
    11161 ttcgcgcgga taatcaggtc acacacttcg cgcaccgcgc ccgcaccgcc ggggcgtttg
    11221 gcaatatagg cggcgtgttg cagcgtaaac caatgcgcgt cggggacggc aaccggcaat
    11281 ccgcagcgca ccatcaccgg caaatcgacc acgtcgtcgc cgacaaaggc gcactcggct
    11341 tcttccaagc ctgcctgcgc gcgcagctct tcatacgcgg cgcgtttgtc cgaaatgcct
    11401 ttgaagtagt aatttatgcc caactgtttg acgcggatgc cgacggaggg cgcgtcccga
    11461 cctgtgataa tcgcagtctg cacgccgctt gcctgaagca ttttcaggcc gtgtccgtcc
    11521 agtgtgtgaa acgatttgat ttcttcgccg tcgtcgcgga taaagatgcg cccgtcggtc
    11581 aaaacgccgt ccacatccaa tatcagcagt ttgattttgg cggcgcgtgc ctgtaattcg
    11641 ggagaaattg cctgcattgg gatttccttg ttttcagacg gcatatctta ccacaaatgc
    11701 cgtctgaaac ggctgcaacg ccttatacaa tccgcgccgc caaaaggtcg tgcatattca
    11761 gcgcacctgt cagcacgccg tctgcatcgg taaccagaag cccgttcaca tggtttgcct
    11821 gcatgacttt cagggcttcg gtggcgaggc gttcggcgga gatggtttta gggtgtgtat
    11881 gcatgacttc gtctatcgaa agaccggtaa aattgtcgca ttcttgaaac aggcggcgca
    11941 aatcgccgtc agtaaacacg cctttcagac agccttgccc gtccgttacc gccaacatgc
    12001 ccaaaccttt ctcgctcatg ctgacgatgg ctcctttcaa gggcgtgccg agtcggacgg
    12061 cgggcaggcc gccgcctttg tgcataatgt cggcaacgcg caaaagcagg cgtttgccga
    12121 ggctgccggc agggtggatc aaggcgaagt cgtcgggcgt gaacgcgcgg gcgcgcagca
    12181 ggacgaccgc caacgcgtcg cccaaagcca taacggcggt ggtgctggtg gtcggggcaa
    12241 gccccaacgg gcaggcttct tgcgaaaccg atgcggtgat gtggatatcg gcatggcgcg
    12301 ccatggttga atcggggcgg gcggtgatgc agacgagggt aatgtctttg cgtttgagcg
    12361 cggggatgat ggcggtgatt tcgtcgcttt cgccggaatt ggaaatggcg gcgacgacgt
    12421 cgttgtccac aatcatgccc aaatcgccgt gtgccgcttc cgcagggtgg acgaaaaacg
    12481 cgggcgtgcc ggtcgaggcc atggttgccg ccattttgcg cccgatatgt ccggacttgc
    12541 ccatacccgt gatgacgacc ctgcccttgc agtgcaacaa cgcgtctgcc gcaaggacga
    12601 agttttcgtc caattccgcc gcaatttcgc gcaagccttc cgcttcggtg tgcaacactt
    12661 cgcgcgccca gtcgagatat ttttcgtttt ctgccattgc catccgcctt tgctgtaaca
    12721 aaactaccca aaatttccga atcggggtag cataagcctt caaaccccgc ccatccatcc
    12781 aaaggaatca aaaatgacta ttttatcgga cgttaaagca ttaggacaac aaatctggct
    12841 ggacaacctt tcccgctcgc tcgtgcaaag cggcgaattg gcgcaaatgc tcaaacaagg
    12901 cgtgtgcggc gtaacttcca atcccgccat tttccaaaaa gccttcgccg gcgacgcgct
    12961 ttatgccgac gaggtcgccg ccctcaagcg gcaaaacctc agccccaaac aacgctacga
    13021 aaccatggcg gttgccgacg tacgggcagc ctgcgacgtt tgccttgccg aacacgaatc
    13081 caccggcggc aaaaccggtt tcgtcagtct cgaagtttct cccgaacttg ccaaagacgc
    13141 tcaaggcacg gtagaagaag cccgccgcct ccatgccgcc atcgcgcgta aaaacgccat
    13201 gattaaagtg cctgccaccg acgcaggcat cgatgcgctc gaaaccctcg tttcagacgg
    13261 catcagcgtg aacctgaccc tgctgttctc acgcgcccaa accctcaaag cctacgccgc
    13321 ctacgcgcgc ggcattgcca aacgcttggc agccggacaa agcgttgccc atatccaagt
    13381 tgtcgccagc ttcttcatct cgcgcgtgga cagtgcgctg gatgcaacgc tgcccgaccg
    13441 gctcaaaggc aaaaccgcca tcgcccttgc caaagccgcc tatcaagatt gggaacaata
    13501 tttcaccgcc cccgaatttg ccgcactgga agcccaaggc gcaaaccgcg tgcagctttt
    13561 atgggcatct accggcgtga aaaacccagc ctatcccgac acgctctacg ttgacagcct
    13621 gatcggcgtg cacaccgtca acaccgtccc cgatgccacg ctcaaagcct ttatcgacca
    13681 cggcacggcg aaagcgacgc tgaccgaaag cgcggacgaa gcactggcgc ggctcgccga
    13741 aattgccgcg ctcggcatcg atgtcgaaac cttggcggcg cgtttgcagg aagacggttt
    13801 gaaacagttt gaagaagcct ttgaaaaact gctcgcgcct ttggtttagg catttccccg
    13861 atgccgtctg aaaggctttc agacggcatc gcccgtttga ccggacacag tgcggcatat
    13921 caaggatccg ccctaaaaat attaatgggc aagaagttaa tgcacgacat tacccgccaa
    13981 cacaaaagga aatagaatca tggcaaaagc actcgaaatc atttcacccg atgaaattta
    14041 ttcagatctg atttttaagg atccggtacc tccccatact atatatacag aattgatgaa
    14101 actggttggt agaaatgcag gcaatgagag aattgcagat agagccttcg attttttctc
    14161 gcctgctctt atggatgatt cagctaccga agagcaatat aatgccctat atgatttaac
    14221 cttgcttgaa gagcccggga tggaactgga taaagatgag ataacggctt tgattaatag
    14281 tcttaaataa aggggctgta ccggatatgt acacaggacg cttcgccccc agcccgaccg
    14341 ggttgctcca catcggctct cttctgaccg ccgtcgcttc ctatgccgat gcacgttcaa
    14401 acggcggcaa atggctggtc cgtatggaag acctcgatcc gccgcgcgaa atgccggggg
    14461 cggcaagcca tatcctgcac acgctcgagg cattcggatt cgagtgggac ggcgaagtca
    14521 cctatcagag ccgccgttac gccctgtatg aagaaaccct ataccgtctg aaaaccgccg
    14581 gactggtcta tccctgccat tgcaaccgca aagactggca ggccggggca aggcggggca
    14641 cagacgggtt cgtctataac ggacgctgcc gccaccccgg ccaacgccct gcactgcaag
    14701 gcaaacagcc gtcgtggcgc atccgcgtcc ccgaccgcga catcggcttt tcagacggca
    14761 tcgtcggcag ttacacccaa aacctcgccc gcgacatcgg cgatttcgtc ctgttccgtg
    14821 cagacggtta ctgggcatac cagctcgccg tcgttgccga cgatgccgaa cagggcgtta
    14881 cccacatcgt gcgcggacaa gacctgctcg tttccacacc gcgccaaatc tatttgcagc
    14941 agtgtttggg cgttccgaca ccgcaatatg cccacctgcc gctgctgacc aacgcgcaag
    15001 ggcagaaatg gtcgaagcag acgctcgccc ccgcattgga tttaaaccgc cgcgaacaac
    15061 ttctccgcca agtgttccgc tacctcaagc tgcccgaagc accggaaacc gaccgccctg
    15121 ccgaattgct cgactgggcg gtggcacact gggatatgga caaagtgccg aaacacgcca
    15181 ttaccgcccc ctaaacacca cccgacaaac gcacaaatgc cgtctgaaac cttcagacgg
    15241 catttcacac atacagcccc gccctattcg cgcgtatttg cccgttcgac ttctttccac
    15301 gcttcgaaaa tcaggccgcc gtcgttgtct ttcaagagcg ttgcatcgga aagcatacgc
    15361 cgccaagtcc gcgcgccttt caaaccgtgc atcagcccga ggctgtggcg gacgatgtgg
    15421 cgcaggattg tgccgcgtcc ggcttggatt tggacttggc tgtatgtgta gaggcgctgc
    15481 accaaatcgg cgtattcgat cggggcgcgg ttgtcaccgt aaaacagcct gtcccattcg
    15541 cgcatcacca tcgggttgtg gtatgcctcg cgcccgacca tcacgccgtc aacgtgttgc
    15601 aagtgtccgg cgattgcttc gttggtggtg atgccgccgt tgatgatgat ttccagctcg
    15661 ggaaactcct gcttgaggcg gtaaacgtaa tcgtatttca agggcggaac atcgcggttt
    15721 tctttgggcg aaagaccgtc cagccaagcg ttgcgggcgt ggacgatgaa ggttttgcag
    15781 gcggttttgt cgcgcagcgt gccgacgaaa tcggcaacgg tttggtattc ggtctgcctg
    15841 tccacaccga tgcggtgttt gacggtaacg gggatgccga ccgcgtcgtg catggcgttg
    15901 aggcagtcgg caaccagccc gacttcgttc atcagacacg cgccgaacgc gcctttctgc
    15961 acgcgcggac tgggacagcc gcagttgagg ttgacctcgt tgtagccgta ttcttcggcg
    16021 gctttggcgg ctttcgccaa atcggacgga tcgctgccgc ccagttgcag ggcgacgggc
    16081 tgctcgcctt cattgaacat caaaaagcgg tctttgtcgc cgtaaacaat cgcgccggcg
    16141 ttgaccattt cgctgtacag ccaagcattt cgggtaatct ggcgggcaag gtaacggtag
    16201 tgcctgtccg tccagtcgag catcggggcg acgcataaac gccttttccc tttatgttca
    16261 ataagttcct tgtttatcat aaggttttca cattctttta actcaaacat tctttattat
    16321 ttgcttggat ttttgcccat ttcactatat ttgacgtacc atacttgcac cataattttt
    16381 aattcttgga atggtgcaaa tggcaacaat aaccaagcgg cgcaatcctt ccggcgaaac
    16441 agtatatcgg gttcaggtac gggtcggcaa gaaaggctat cctgctttca atgagagcag
    16501 gacgttcagt aaaaaggctt tggcggtcga atgggggaag aaacgggagg cggaaatcga
    16561 agccggccca gaactgcttt tcaagcgcgg caaggtcaag atgatgacgc tgtccgaagc
    16621 tatgcggaag tatcttaacg aaacgcttgg agcgggtcgg tcaaagaaaa tgggcttgcg
    16681 ttttctgatg gagttcccga ttggcggcat cggcatcgat aagctgaaac ggtctgattt
    16741 cgcggaacac gttatgcaga ggcggcgcgg aatccccgaa ctggacattg cgccaatcgc
    16801 ggcttctacg gcattgcagg agctgcaata tatccgttcc gtgctaaaac acgcctttta
    16861 tgtgtggggg cttgaaatag gctggcagga attggatttc gcggcaaacg ggctgaaacg
    16921 ctcgaatatg gttgcgaaat ctgcaatcag ggacagattg ccgaccacgg aagaactgca
    16981 aaccctgaca acttatttcc tgcggcaatg gcaaagcagg aaatcttcca taccgatgca
    17041 cctgattatg tggctggcga tttatacgtc aaggcggcag gatgagattt gccgcctgct
    17101 gtttgacgac tggcacaaaa atgattgtac ccggccggtt cgtgatttaa aaaatccgaa
    17161 cggcagcaca gggaataata aggagtttga tattctgcct atggctttgc cggtcattga
    17221 cgagctgccg gaggaatcgg tcaggaagcg tatgcttgcc aacaaaggca tcgccgacag
    17281 ccttgtaccg tgcaacggaa aatcggtttc cgccgcttgg acgagggcgt gcaaggttct
    17341 tggaattaaa gacttgcgct tccacgattt acggcacgag gctgctaccc gtatggctga
    17401 agacggcttc acgattccgc aaatgcaacg ggtaacgctg catgacggtt ggaacagcct
    17461 gcagaggtat gtgagcgtac gcaaacgctc gacgcggctg gattttaaag aggcaatgat
    17521 gcaggcgcaa agcgatataa aatccgggaa gtgatgttaa attaaagggg atgtgccgca
    17581 tcccctgatt cctccgtctt gcatcaagac cggaatttcc gatattccga ttttgccttt
    17641 tcctccttgt ctgcgattgc ctttgcaagc tctcggacgc tgacgaaata ttccgatttt
    17701 cggctgtcgg acaatcggaa aatcggaaac ggaagctcgc accgcacccc cgatgcttta
    17761 aacgcgttaa agccgatatg ggcaaaaaag tctttgtgta cttccctcgg cgggatatac
    17821 gggcggccgc cgtatgcgat taaaagccct tgttctttac tgatgttcat atatacctct
    17881 ttctgttaat tctgctttgt ccgggtttcc gcttttttac ggcttggcgg atgttcagcc
    17941 tgcgtcggtg cataagtcct cctgttcggg cggttgttca gccggccgcc cctccgaaaa
    18001 gtggcaggct gcacgggaag tagtgttttt ttccgtattt ctctgcctgt atcaggttgc
    18061 acaggcggtc gcaaaagccg tcaaattcgg catgcggcca gccttctatc tcgttgacca
    18121 ttttgctgaa cctcaattcc cgggcaatcc gctttgccgc tgcgtcacgg cattcgtgtt
    18181 ccaagaggac ggggtcttcc ttttccttcg gcggtgcagg ttcttgcacg gtttgccaca
    18241 agtccggttc gatttgccaa gtgtcggcgg caagcagggt gtctgttttg cagtcgtaca
    18301 gttcgcggat gcagccgccg tcggttttgt ctttgtcgat aaccagtaaa agcacttcga
    18361 tcggcgtgtc ttcaaacgcg ttttgtatgc ggttgagttc tgccagccgg ttgccgatga
    18421 tttcgcggaa tcgctgctcg cttttgcggt aggcgatgcc ggggaacagg atgaagaatc
    18481 cgaagcggcg ggcgttttcc aatcctttta aaacgaagat ttcatcggct acgcccgatt
    18541 tcttccacgg gtattcccgt gcgatacggc ttttttcgtc ttcgctcaaa tcctttagct
    18601 tgatggaaaa cggcggattc atgacggtgc agtcttgcgg ttcgccgtcg gtataaagga
    18661 aaaggcttgt gttgtatact ttggcggcag ggtagttttg caacaaggct ttgcacgctt
    18721 ccgcctgtat ctctacggcg cggaaatcgg acggttcgat gtactgctcc agctgcccgc
    18781 ttcctgccgc gccgtcaaat acgcccgggt gttcgccgca gtatcggcgg actttggcgg
    18841 caaccagccg cctcaagctg ccgcccgtga tgtattcggc gtagccgccg gcttttttac
    18901 ggttgttgtg tccttgaaac gtcatgggtt ttcgtccgtt ctttggcttg tccgggtgtg
    18961 gcggagcgtt tgcggatgcg gctgacgcgc tgcggtttgc cgtcttcgcc gcgctgaaaa
    19021 acgcttgttc cgccgcgccg gcagccgtgc cgcttatgcc tgatgccgtg tttgaagcct
    19081 tttgtgccgg aatcgtgata cacgcaggtt cggatgtgcg tcctcatgcc gccgcttccc
    19141 tgtgcatgag gctgatcagg tttgccgtcc gtttgaaatg ttcggtccag ttgaagcagc
    19201 tgaagccggc gccgttgtcg caatgccgga tgacgtcttc cgagtctttt acggcccgga
    19261 ccaacgcgcc gcctttgtcc gcaacaagca ttttcatcat cggccggcgc acgtcgctta
    19321 tccgtcgggg gcgcacggga ttcagcggct tgccgtacat cgccgaaagc tccttgtccg
    19381 cctgaatgcc cacatcgtaa agcaggtttg cggcgtgtag gatccgacct gtaaattccg
    19441 cgtacggcat tttaaggcgg cgggcttccg cgcgcgaatc cgcgccgttg acgactttgc
    19501 caaccgccga aatcaaaccg ccgtcaagtt ctaaaaacgc catggctttc ttggcaaccc
    19561 gcacggccaa tgagtatgcc gatacttcgg ctatcagccg tgcaggcgcg tctttcagaa
    19621 attcttcgta cgcggcgaag agatttgaca gcggacgcag cacgagttgg cgctgattcg
    19681 ggcttaaatt gtcaaaatct ttagtccatt ttgcgacggc ctgcgatgct tcgcggcagg
    19741 caaacagtac gctttcctcg ttctcggggt cgtccgtgtt gcagtacagg cccaagcgtt
    19801 gcacctgttt gacgatatgc tcggcgaagt tgattaactc ctgattgcag gcgtaacgca
    19861 tatcctgcag ggacagccgc atcatgatgc cgcacgtcag ggcttcgtct tccgatacct
    19921 ttgcgcc
//
LOCUS       DAXFHF010000040        19850 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXFHF010000040 DAXFHF010000000
VERSION     DAXFHF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.040"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..1068)
                     /gene="leuB"
                     /locus_tag="ACFIRM_001315"
     CDS             complement(<1..1068)
                     /gene="leuB"
                     /locus_tag="ACFIRM_001315"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC3028937.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            complement(1102..1834)
                     /locus_tag="ACFIRM_001316"
                     /pseudo
     CDS             complement(1102..1834)
                     /locus_tag="ACFIRM_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            complement(1850..3121)
                     /locus_tag="ACFIRM_001317"
     CDS             complement(1850..3121)
                     /locus_tag="ACFIRM_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3028938.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            complement(3288..3929)
                     /gene="leuD"
                     /locus_tag="ACFIRM_001318"
     CDS             complement(3288..3929)
                     /gene="leuD"
                     /locus_tag="ACFIRM_001318"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC3028939.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            complement(3991..4245)
                     /locus_tag="ACFIRM_001319"
     CDS             complement(3991..4245)
                     /locus_tag="ACFIRM_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028940.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            complement(4340..5749)
                     /gene="leuC"
                     /locus_tag="ACFIRM_001320"
     CDS             complement(4340..5749)
                     /gene="leuC"
                     /locus_tag="ACFIRM_001320"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC3028941.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            6001..7360
                     /gene="gshA"
                     /locus_tag="ACFIRM_001321"
                     /pseudo
     CDS             6001..7360
                     /gene="gshA"
                     /locus_tag="ACFIRM_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            complement(7334..7456)
                     /locus_tag="ACFIRM_001322"
     CDS             complement(7334..7456)
                     /locus_tag="ACFIRM_001322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028942.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            7483..8160
                     /gene="radC"
                     /locus_tag="ACFIRM_001323"
     CDS             7483..8160
                     /gene="radC"
                     /locus_tag="ACFIRM_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC3028943.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            8233..8958
                     /locus_tag="ACFIRM_001324"
     CDS             8233..8958
                     /locus_tag="ACFIRM_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC3028944.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            9110..10390
                     /locus_tag="ACFIRM_001325"
     CDS             9110..10390
                     /locus_tag="ACFIRM_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC3028945.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRHAQAKWQF
                     RTRKPDYPHFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            10396..11574
                     /gene="hflX"
                     /locus_tag="ACFIRM_001326"
     CDS             10396..11574
                     /gene="hflX"
                     /locus_tag="ACFIRM_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC3028946.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            11571..14042
                     /locus_tag="ACFIRM_001327"
     CDS             11571..14042
                     /locus_tag="ACFIRM_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3028947.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(14701..15477)
                     /locus_tag="ACFIRM_001328"
     CDS             complement(14701..15477)
                     /locus_tag="ACFIRM_001328"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3028948.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(15665..16459)
                     /locus_tag="ACFIRM_001329"
     CDS             complement(15665..16459)
                     /locus_tag="ACFIRM_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028949.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLALRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            complement(16506..17915)
                     /gene="thrC"
                     /locus_tag="ACFIRM_001330"
     CDS             complement(16506..17915)
                     /gene="thrC"
                     /locus_tag="ACFIRM_001330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC3028950.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            complement(17988..18356)
                     /locus_tag="ACFIRM_001331"
     CDS             complement(17988..18356)
                     /locus_tag="ACFIRM_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028951.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            complement(18607..19850)
                     /locus_tag="ACFIRM_001332"
                     /pseudo
     CDS             complement(18607..19850)
                     /locus_tag="ACFIRM_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
BASE COUNT         4642 a         5202 c         5234 g         4772 t
ORIGIN      
        1 caaggcggcg agtaccgcgt cgcccatttc ggagcaggaa acgagttttg tgccttcttc
       61 gtaaatatcg ccggtacgca agccttgttg cagcactttt tggacggcgt tttcgacttg
      121 ttgcgcgcgc gcttcgtcgt tcaggctgta acgcagcaac atggcaagcg agaggatggt
      181 ggccagcggg ttggctttgt tttgtccggc gatgtcgggg gcggagccgt gagacggttc
      241 gtacaggcct ttgccgtttt cgtccaaaga agcggaaggc agcataccga tggagccggt
      301 cagcatggag gcttcgtcgg agaggatgtc gccgaagatg ttgccggtgg caatcacgtc
      361 aaactgtttg ggcgcgcgca cgagttgcat ggcggcattg tccacataca tatgggaaag
      421 ctcgacatcg ggatattgtt tgccgatttc ttcaaagatt tcgcgccaca gttcggtggt
      481 ttccaaaacg ttggctttgc ctacggagca gacttttttg ctgcgttttt gggcggattg
      541 gaaggccacg tgggcgatgc ggcggatttc gctttcgctg tatttcatgg tgttgtagcc
      601 ttcgtgttcg ccgttttcca aaacacggat gccgcgcggt tcgccgaaat aaatatcgcc
      661 ggtaagttcg cgcacaatca aaatatccaa accggcaacg atttcaggct tgagcgtgga
      721 ggcgttggct aattcgggat ataaaacggc aggacgcaaa ttggcaaaca ggttcaaatc
      781 cttacgaatt gctaacaagc cgcgctcggg gcgcaacgga cggtcgagat tgtcgtattg
      841 gggcgaaccg actgcaccaa gcaggacggc atcggctttg cggcagaggt tttgcgtaaa
      901 ttctggataa ggatggccgt attcgtcgta ggcttcgccg cccaaaggcg cgtattcgta
      961 gtcggcatcc aagccttggg cgataaattt gtcgagtacg cggacggttt cggcgacgat
     1021 ttcgggaccg atgccgtcgc ctcggaggat ggcgatatgt ttggtcattt caagtttcct
     1081 tatgggttga tggttgaagg gttatttctt tttgtatttg tgtgcaattt cgtgccaacg
     1141 gggtatggaa atcgatcggt tgtagtgttt tttataagct tcctcaaatt tctttttcca
     1201 taaggatgcg ttatgccgtg ttgccgggtt ttgataaacg gtttcttcaa ttgcggaaac
     1261 aaaatcttcc aagcattcaa acataggcat attcttttta tttatgctgt accaaacacc
     1321 cgggcggatt ttatgcacca cgcctttttt gacttgaaga ttaattgccc acccatccat
     1381 gcttctggta atttgccata cttttttaag ccataaatcc ttgatggtta cattgccgtt
     1441 gtcgtccata tcgtaaccga atattaaata atctacatcc agcatatagg gcttatgaat
     1501 gatttcatca gaatacattt taaaatctgc aatatcaaaa cccgggcagg catttcggtt
     1561 gaacgccttt acttccaaca attctctgct gcggcctttt ttatttaaaa aaaaaatcgg
     1621 gaggcatttg ggtattggtt gaaacatcaa attcaatttc cctttttctc aaccatccgc
     1681 cgagccattc ctgaatgatg ttgccgacaa catctttttg tttgacgata atatccacat
     1741 cgcctaagaa aaatctaatt tgaccattgg ccgataagat tttttcttca tccagcaact
     1801 tatcaaatat ttgttgtgca gtaagtttta tcattttatc cctgtcggtt tataaagtat
     1861 gcagaagtct ttcagatacc gccttaatca cagggacggc aacggtatta cccaataaat
     1921 cgcatctgtc ttttttggga atatcaaacg aataatcgtc cggatagccg aataagcgta
     1981 acccttcttt tccggtaagt gtacgcaaac cgccgttgtc aacgacgaaa aggtgctcca
     2041 tatccattgc aaccaaggtt ggcgcaacat cgtttggatc taatatttta ttgatttcaa
     2101 aagatttttt acctgaaaca atattgtagc ctttgggcag ggattcatct ttgattcttt
     2161 gcccgccaat tttttgtttc ggatgctcta aaaccaaata gcctttgtct gtcaggctgt
     2221 ccaaaatatt ttggagatcg gggtgtttat agaaagttga aatttgcgct tttgtcaaag
     2281 gcatcccatc catccaatct atgccgattt ctgaagccca tttttttttc ctccgttctt
     2341 ttagaaggat atttaacaat tgcttctctt cttcggttac tgcgcctttt aattcaatat
     2401 cccaactgtg aatattgttt ttccctcccc gtttgtcttt tactgatttt ccgtacagtt
     2461 cggacggggg aaattttttt agcaattttt tgatgaaagg gctgctttca gtaggcagtc
     2521 ccgattccaa aatatttttt aatttcggac ttgggcttgt ttcgaaagat aagtcgggtt
     2581 tggatttcaa actgcctgtc agataaatgc gcttcctgtt ttggggaatg ccgaaatctt
     2641 ttgcatttaa aactttccaa gaaacatagt agcccaatgc ttccaaggtt tccaaaataa
     2701 cggtcagggt gcgccctatt ttttgtgtcg aatcttttct atcgtgtgtc accaatcctt
     2761 ccacattttc caaaataaaa ccttttggtt tttttgcctt taaaatcctt gccacatcaa
     2821 agaaaagcgt tccccgtgta tcttcaaagc ctaatctttt tccggcgaaa gaaaaagcct
     2881 gacaagggaa gcctgccaac aggatgtcaa aatcgggaat atcccctgtt tcaattttcg
     2941 ttatatctcc atacggcact tcatcagggt agttttgctt caatacttcc aaagctgccg
     3001 gtttgatttc tgaagtaaaa acacattcgc aagcaaccga ctgtttccga caggcttgtt
     3061 cgaatccttt cctgatgcca ctcattccgg aaaataagtc aataaattta atttgttgca
     3121 tattaaaaat ctaaaaattt atttgaaatg gagagttgca ttaatttaga gtgttgctaa
     3181 gcccgcttaa aagatgaaaa caatttatcg cccctctgtt tacattagcc gcagcaatta
     3241 tatgttatca ggaatgccgt ctgaacagcc ttcagacggc ataggtttta accgttaaac
     3301 agccaaggct ggctttggcg gcgtttttct tcaaaggctt taattttgtc ggcgtgttgc
     3361 agggtcagtc cgatttcgtc caagccgttt aagaggcagt gtttgcggtg ttcggtaatg
     3421 tcgaatgtga atgtttcgcc gcccggggtg gtcagggttt gctcggcaag gtcgatggag
     3481 agccgatagc cttcgttggc ttcgacttct ttgaaaagcc ggtcgacttg ttcttcggtc
     3541 aacacgatgg gcaaaaggcc gtttttgtag cagttgttaa agaagatgtc ggcgaagctg
     3601 ggggcgataa tggcgcggaa gccgtagtcg tccaatgccc aaggggcatg ttcgcgtgaa
     3661 gagccgcaac cgaagttttt acgcgtcaac aggatttgcg cgccttggta acgcggctgg
     3721 ttcagggaaa aatcggggtt caacgggcgt ttgccgttgt ccatgcccgg ttcgccgtgg
     3781 tcgaggtaac gccattcgtc aaaggcgttg gggccgaagc cgctgcgttt gatggatttc
     3841 agaaattgtt tggggatgat ggcatcggta tcgacgttgc tgcggtcgag cggggcgacg
     3901 atggcggtaa ttttggtaaa ggctttcatg ggtttgcgtc ttgtgctgac gatgccgtct
     3961 gaaacggttt cagacggcat cgcaaatcgg ttattcggtg gcgttttcga tttttttgcc
     4021 gagatgggaa atgccgcgtc cgacggcatt gccgcctttt ttgacggctt ctttggtttt
     4081 gtcccaacct ttttcgacgg cgttgcctgt ttgttcggcg gcgcgttcgg cggcggcctg
     4141 tgttttgtca aggttgcggg cggtgtcttg tttcgcgccc tgccaagtgc cggcgcaggc
     4201 ggacaaggcg agggcggaca gggcggtaac gaaaagtttg ttcatggtta aactccttgg
     4261 tttgaatatt aggtgtttct gccttacggg acatatttca gacggcctcg tcaaattctt
     4321 aaagactgcc tgaaaatact tacgccatcg tgcggatgtc ggtaaagtgt ccggtaacgg
     4381 cggcggcggc ggccatagcg gggctgacga ggtgggtgcg cccgccgttg ccttggcggc
     4441 cttcaaaatt gcggttggac gtggaggcgc agcgttgcct cggcgcgagg cggtcggcat
     4501 tcatcgcaag gcacatcgaa cagcccggtt cgcgccattc aaaaccggct tcgatgaaaa
     4561 ttttgtccaa gccttctttt tcggcttgtt ctttaaccaa accggagccg gggacgatta
     4621 acacgcgctg tacgttgccg gcttttttat ggcctttggc gatagcagcg gcttcgcgca
     4681 agtcttcgat gcggctgttg gtgcaagagc cgataaagac gatatcgaca gggatttcgt
     4741 tcagcggcgt accggccttc aaacccatat attcgagtgc gcgctctatg ccgctgcgtt
     4801 tgaccggatc ggtttcttcg gcgggattcg gcactttgcc gccgatgttt aaaaccattt
     4861 cgggcgacgt gccccaagta acttgcggtt cgatgtcttc ggcgttgaaa cggtattctt
     4921 tatcaaaaac cgcgccttcg tcagacacca gcgtgcgcca gtactcgacg gctttgtccc
     4981 aagcttcgcc ttcgggtgcg aagggcttgc ctttgacata gtcgatggtg gtttgatcga
     5041 cggcaaccat accggaacgc gcgcctgcct caatcgccat attgcacagg gtcatcctgc
     5101 cttccatcga aaggctgcgg atggcttcgc cgccgaactc gacggcgtaa cccgtgccgc
     5161 ccgccgtgcc gatttgcccg atgatgtaga gcgccacgtc tttggcggta acgcccgctt
     5221 ttaatttgcc gtcaacggca atcagcatag atttggattt tttcgcggta atgcactggg
     5281 tcgccatcgt gtgttcgact tcggaagtgc cgatgccgtg tgccagtgcg ccgaatgcgc
     5341 cgtgggtgga agtgtgcgaa tcgccgcaga cgacggtcat cccgggcagg gtcgcgcctt
     5401 gttcgggccc cataacgtgt acgatgccct ggcctttgtc cataaacggg aagtaggcga
     5461 gtgcgccaaa ctctttgatg ttttgatcca acgtatcgac ttgcagcttg gaaatcgggt
     5521 cttggatgcc tttgtcccaa tcgccggtcg gggtgttgtg gtcggcggtg gagaccacgc
     5581 tgtcgatgcg ccacagcttg cgccccgcca ttttcagacc ttcaaacgcc tgcgggctgg
     5641 ttacttcgtg aaccagatgg cggtcgatgt aaagcaggac ggtgccgtct tcttcttcgc
     5701 ggacgacgtg gctgttccag agtttgtcgt agagggtttg tgccgtcatg atgttgttct
     5761 tttggataaa tggtgatgcg gattgggcgg attttagacg tattctttat actgcgcaac
     5821 agattttgtc taatttttga gtcggtgtta ttttgtaaac aattttaacg aaaaaattag
     5881 acatattgtt catttcgtta tatctaaaga atgattcgat acgaaagaat acttgtcgtc
     5941 attctttcaa aagcattatt atctgtacct tgtcaaaaaa cacacagagg cagacgaaag
     6001 atgaaattac cggttatgtc gcccgaacat tcggcgcaac ttcaggcgtt tgaggcaaaa
     6061 atcctgtcca atcacgccaa aatcgaggcg tggttccgca cgcagtggaa cgcgcaccgc
     6121 ccgccgtttt acggttcggt cgatatacgc aacgccggtt acaaaatttc gtctatcgat
     6181 atgaatttgt ttccgggcgg cttcaataat ctgaatccga atttcatccc gctggcggcg
     6241 gttgccgcgc aagatgcggt gcaacgcgcc tgcgaaacgg agaaatccgt attgattatt
     6301 cccattattc ccgaaaacca cacgcgcaat acgttttacc tgcaaaacgt ttacgccctc
     6361 ggcgagattt tccgttcggc agggtgtgaa gtgcgcttgg gcagcctgaa tccggaagta
     6421 accgaaccgg ccgaatttga aactgcattg ggcgacaaaa tcctgcttga gcctttgctg
     6481 cgaacccgcg accgcgtcca ccttgccgac ggcttttcgc cttgcgtggt tttgttgaac
     6541 aacgatttgt ccgccggcat tcccgacatt ctcaaaggca tcggccaaac cgttttgccg
     6601 ccgctgcacg gcggttggac gacgcgccgc aaaacaaatc atttcggcgc gtacaaccaa
     6661 gttgccgccg aatttgccaa gctgattgac atcgatgaat ggcagatcaa cccgtatttt
     6721 gaaaaaatcg gcggtttgga cttccaaggg cgcgaaggtg aagatgcgct ggcggaagcg
     6781 gtagaacgcg tgctggcgaa aattcaagcc aaatacgacg aatcgggcat tgccgacaaa
     6841 cctttcgtca tcgtcaaagc cgatgccggc acttacggca tgggtgtgat gagcgtcaaa
     6901 tcctccgacg aagtgcgcgg cttgaaccgc aaaaaccgca acaaaatggc gaaggtgaaa
     6961 gagggcttgg aagtcagcga agtgattgtc caagaagggg tttataccta tgaaaccttg
     7021 aacggcgcgg tgtgcgaacc cgtcatcata tatgatggac cgtttcgtca tcggcggctt
     7081 tttccgcgta cacgaagggc gcggtgcgga cgaaaacctc aacgccggcg gtatggtgtt
     7141 tgtgccgctg tccaacagca ttcctaccgg taacggcgat aattcgcaag aagctccaga
     7201 agcctgcaaa cgcgtattcg aacaatggga ttcgctcggt atgccgcgtt ctgaaaaaga
     7261 ctgcgacgtg gacaacgaac ataaccgcct ctacgtttac ggcgtaatgg cacgtctgtc
     7321 gcttttggcg gcttcaatcg agttggaaga aacggcgtaa gactgttttg aaatacagat
     7381 gccgtctgaa gcggaaatcc ggttcagacg gcatttcgga tatttggcgt gtgggaacat
     7441 ctgtttcaga cggcatctca gactatttaa aaaagggaaa acatgagcat caagcaatgg
     7501 ccggaaggcg aaaggcccag ggaaaagctg ttggaacgcg gggcggcggc tttgagcgat
     7561 gccgaacttt tggcaatcct tttacgcgtc gggacgcgcg ggatgagcgc ggtcgatttg
     7621 gcgcgctacc tattgcagga gttcggcagc ttggggaggc tgatgagcgc ggaggtcggc
     7681 aaactgtcgg catacaaagg gatggggacg gcaagtttca cacagttcgc cgtggtcagg
     7741 gaaatcgggc ggcggatatt ggaggaagaa ttgcaggaag aaattacctt atccgatccc
     7801 gataccgttg ccgattattt acgctttcat ttggggcagg aaaaagtcga agtcagcgtc
     7861 gcgctgctgc tgaaccgcca aaaccaactg attgcggtca gagagctgtc gcgcggtacg
     7921 gttgcggaaa acacgattta catccgcgaa atcgtcaaac tggcattgga cgaatatgcc
     7981 gacagcctga ttatcgcgca caaccatccg ggcggctcgc ccgaaccttc gcaggaagac
     8041 atcatgttca caaggcggct ggcacaggcc atgtcgctgg ttgatgtttc gctgctcgac
     8101 cattttatcg ttacctcgca aaccgtccgt tcgttcaggc agctcgggct gatgccctga
     8161 caccctgttt tacattcggc ggctctggta aaatagccgc tttgaccgta ttcgacagat
     8221 attgttaagt taatggaaac acaaaacaaa cctaccgtta ccgacattga ccgccccata
     8281 ctcgtcccgc ccggcggaca taaaaaagtc ttgctgcatt cctgctgcgc cccgtgcagc
     8341 ggcgaagtga tggaagccat gcttgcctcc ggcatcgact acaccattta tttttacaat
     8401 cccaatatcc atccgcacaa agagtatatg ctccgaaaag aggaaaacat gcgctttgcg
     8461 gaaaagttcg gcatcccttt catcgataaa gacgacgact acgaaaacga ccgcaaagaa
     8521 tggtttgcca aagccaaagg catggagttt gagccggaac gcggcatccg ctgcaccatg
     8581 tgtttcgata tgcgttttga aaaagcggcg caatacgcgc acgaacacgg ttttcccgtc
     8641 tttaccagtt cgctgggcat ttcacgttgg aaaaatatgg cgcaaatcaa cgactgcgga
     8701 caccgtgccg ccgcgcctta cgatgatgtg gcctattggg acttcaactg gcgcaaaggc
     8761 ggcggcggcg cgcgtatgat tgaaatcagc aaacgcgaaa acttctacca gcaggaatac
     8821 tgcggctgcg cctattcctt gcgcgattcc aacgcccacc gcaaatcgca gggacgcatc
     8881 cccgtcaaac tcggcgtgct gtattacggc gacgaatcga cacaatacga acctgcaccc
     8941 gtccgagtgg acaaataaac acccgatgcc gtctgaaggt tcagacggca tcgggttcgg
     9001 caccggcacg ggaaaggttt gccggtttgg caatcggcaa gcggaaaccg cattggcaag
     9061 tttgccgttt tgataaaaca ccccgttgcc gcgtcgggag ggcggcatta tgaaatccct
     9121 ttttatttgg ctgcttctat tgggctcggc ggcaggcgtt ttctaccata cccaaaacca
     9181 atccctgccc gcgggcgaac ttgtctatcc gtccgcaccg caaatcaggg acggcggcga
     9241 tgcgctgcac tacctcaacc gcatccgcac acaaatcggt ttgcacgcgc tggcacacgc
     9301 gccggttttg gaaaattccg cccgcaggca cgcacgctat ctcacgctca atcccgaaga
     9361 cggacacggc gaacaccatc ccgacaatcc gcactacacc gcacaaaagc tgaccgaacg
     9421 cacacgcctt gccgggtatc tctacaacgg cgtgcatgaa aacatcagca cggaagagga
     9481 agccgccgaa tcgtccgaca gcgacatccg cacgcagcaa cgccaagtgg acgctttgat
     9541 gagtgctgtc tatcaccgcc tttcgctgct tgaccgccat accgacgaag caggtgcggc
     9601 atttgtgcgc gaaaacggca aaaccgtcct cgtattcaat cagggcaacg gcagcttcga
     9661 gcgcgcctgt gcaaaaggaa ggcggcagcc ggaagcagga cggaaatatt accgcaacgc
     9721 ttgccacaac ggtgcggccg tttatgctga cgaagccatg cccgtaacgg aattgcttta
     9781 taccgcctat ccggttggcg gcggcgcgct gccttatttt tacggggaac gtcccgaccc
     9841 cgtgccggaa tatgaaatca caggcaatcc tgccagcatt gatttttccg aggcggcagg
     9901 caaaattgcg atgaaaagtt tcaagctgta tcagggtaaa aacgaaatcc gccccgtcag
     9961 ggttttaacc gccggcaacg accctaacgg caggctgacc gcgcaccaat tcgccctttt
    10021 cccgctcaaa cctttggaat acggcacgct ttatacggcg gtattcgact atgtccgcaa
    10081 cggacggcac gcgcaggcga aatggcagtt tagaacccga aaacccgatt accctcattt
    10141 tgaggtaaac ggcggcgaga cacttgcggt tagaaaaggc gaaaaatatt tcatccactg
    10201 gcgcggacgc tggtgtctgg aagcgtgtac ccgttatacc taccggcggc agttcggcaa
    10261 cagcctgtcc atactccggc acgaagcggg cggcattgtc ttcagcgtca gcggaatggc
    10321 gggaagccgc atcaggctta ctccggaaga cagcccggaa cgcggtgtaa ccctttattt
    10381 gcaggattga cagttatgtc aggtagaaca ggacggaaca gtgccactca ggcgcaaccg
    10441 gaacgcgtca tgctggtggg cgtaatgttg gataaagatg atacgggcag caatgccgcc
    10501 cgtctgaacg gttttcagac ggcattggcg gaagccgtcg agctggtcaa ggcggcgggc
    10561 ggcgattccg tacgcgtgga gactgccaaa cgcgaccgcc cgcacactgc gctgtttgtc
    10621 ggcacgggca aggcggcgga gctgtcggaa gcagttgccg cagacggcat tgatttggtc
    10681 gtattcaacc acgaacttac tcccacgcag gaacgcaatt tggaaaaaat cctccaatgc
    10741 cgcgtattgg acagagtggg gctgattctg gcgattttcg cccgccgcgc ccgcacgcag
    10801 gaaggcaggc tgcaagtcga gttggcgcaa ttgagccatt tggcgggacg cttgatacgc
    10861 ggttacggac atttgcaaag ccagcgcggc ggtatcggca tgaaagggcc gggcgaaacc
    10921 aaactggaaa ccgaccgccg attaaccgcc catcggatca acgccttgaa aaaacagctt
    10981 gccaacctca aaaaacagcg cgccctgcgc cgcaagtccc gcgagtcggg cagaatcaaa
    11041 acgtttgcgc tggtcggcta taccaatgtc ggcaaatcca gcctgttcaa ccggctgacc
    11101 aagtcgggca tatatgcgaa agaccagctt ttcgccactc tcgacacgac ggcgcggcgg
    11161 ctgtacatca gtcccgcatg cagcattatc ctgaccgata ccgtcggatt cgtcagcgat
    11221 ctgccgcaca aactgatttc ggcattttcc gccacgctgg aagaaaccgc gcaagccgat
    11281 gtgctgctgc acgtcgtcga tgccgctgcc ccgaacagcg gacagcagat tgaagacgtg
    11341 gaaaacgtac tgcaagaaat ccatgccggc gatattccgt gcatcaaggt gtacaacaaa
    11401 accgacctgc tgccgtctga agaacaaaac acgggcatat ggcgcgacgc tgcgggaaaa
    11461 attgccgccg tccgcatttc cgttgctgaa aataccggta tagacgcact gcgcgaagcc
    11521 attgccgagt attgtgccac cgcaccaaac acagacgaaa ccgaaatgcc atgaaaaaaa
    11581 cctgtttcca ctgcggtctg gatgttcccg aacacctcca cctgactgtc cgttacgaaa
    11641 acgaagaccg cgagacctgc tgcgccggtt gtcaggcggt cgcacaaagc attattgacg
    11701 cgggcttggg cagttattac aaacaacgca ccgccgacgc gcaaaaaacc gagctgccgc
    11761 cccaagaaat cctcgaccaa atccgcctgt acgacctgcc cgaagtccag tccgactttg
    11821 tggaaaccca taatggcacg cacgaagcag tgctgatgct cagcggcatt acctgcgccg
    11881 cctgcgtctg gctgatcgaa cagcagcttt tgcgcacaga cggcatcgtc cgcatcgacc
    11941 tcaattacag cacgcaccgc tgccgcgtcg tctgggacga cggcaaaatc cgcctctccg
    12001 acattctgtt gaaaatccgg cagacaggct acaccgccgc accctatgac gcgcaaaaaa
    12061 tcgaagccgc caaccaaaaa gaacgcaaac aatacatcgt ccgccttgcc gttgccggac
    12121 tgggtatgat gcagacgatg atgttcgcgc tgccgaccta cctttacggc ggcgacatcg
    12181 aacccgattt cctgcagatc ctccattggg gcggcttcct gatggtgctg cccgtcgtat
    12241 tttattgcgc cgtcccgttt tatcaaggcg cgttgcgcga cctgaaaaac cgccgggtcg
    12301 gtatggacac gccgattgcc gccgccatca tcatgacctt catcgccggc atttacagcc
    12361 tcgccaccaa cgcagggcag gggatgtatt tcgaatccat cgcgatgctg ctgtttttcc
    12421 tgctgggcgg acgctttatg gaacacattg cccgacgcaa ggcaggcgat gccgccgaaa
    12481 ggctggtgaa actgataccc gccttctgcc accggatgcc cggctatcct gccgtacagg
    12541 atgtccgcga atccgccgtc gtcaaactcc aagcaggcga tattgtgatg gtcaaacccg
    12601 gcgaaaccat ccccgttgac ggcacggtgc tggaaggaaa cagtgccgtc aacgaatcta
    12661 tgctgaccgg cgagagcctg cccgtcgcca aaatgccgtc tgaaaaagta accgcaggta
    12721 cactcaacac gcaaagccct ctgattatac gcaccgaccg caccggcggc ggcacgcgcc
    12781 tgtcgcacat cgtccgcctg ctcgaccgcg ccttggcgca aaaaccgcgt actgccgagt
    12841 tggcggaaca atacgcctca tctttcatat tcggcgaact cctgcttgcc gtccccgtct
    12901 tcatcggctg gacgctgtac gccgatgcgc acaccgcatt gtggattacc gtcgccctgc
    12961 tggtcattac ctgcccctgc gccctgtcgc ttgccacgcc gaccgcgctg gcagcttcta
    13021 ccggtacgct ggcgcgcgaa ggtattttaa tcggcggaaa gcaggcaatc gaaaccctct
    13081 cccaaaccac cgacatcatt ttcgacaaaa ccggcacgct gacccaaggc aatcccgccg
    13141 tccgccggat tgaactgctc ggcagcatga ccgaagccca agtgctggca gtggcgcaaa
    13201 gtttggaaca gcagtcagaa cacccgctcg cgcgcgccat cctcaactgc cgcatttcag
    13261 gcggcagcgt ccccgaaatc caagtcggac aacgcctcaa ccgcatcggg gaaggcgtgg
    13321 gtgcgcaact gaccgtcaac ggcgaaacac aggtttgggc attggggagg gcatcctatg
    13381 ttgccgaaat ttcaggtaaa gaaccgcaaa cagaaggcgg cggcagcgcg gtttacctcg
    13441 gcagtcaaag cggtttccaa gccgtgttct acctgcaaga cccgctcaaa gacagcgcgg
    13501 cggaggcagt gcggcagttg gcaggcaaaa acctgaccct gcacatcctc agcggcgacc
    13561 gcgaaaccgc cgttgccgaa accgcacgcg ccctgggtgt cgcgcactac cgcgcccaag
    13621 ccatgcccga ggacaaactg gaatacgtca aagccttgca aaaagaaggg aaaaaagtgc
    13681 tgatgatagg cgacggcatc aacgacgcgc ccgttttggc gcaggcagac gtatccgccg
    13741 ccgcagcggg cgggacggat attgcgaggg acggcgcgga cattgtgtta ttgaacgaag
    13801 atttgcgtac cgtcgcccac ctgctcgatc aggcgcggcg cacccgccat attatccggc
    13861 aaaacctgat atgggcgggc gcgtacaaca tcatcgccgt accgcttgcc gttttgggct
    13921 atgtccaacc gtggatagcc gcactgggta tgagtttcag ttcgctggcg gttttgggca
    13981 atgccttgcg ccttcacaaa cgggggaaaa tgccgtctga aaaaatactg tccgaacaat
    14041 gacgggcggc gttgctttag acgtatagtc gattaaaaca aaaatgagac gatgaaggat
    14101 tacaaactta aagtatgcat tgttaccgct caaacacgtt ggcgtttaaa atttgagatc
    14161 gaattgctag gtatttgatt ttattaaata agagattata ttaatgagga aaaaaattaa
    14221 aaagtggatt tatgcgataa atggaataat gatgtcaata tggattattt ttttttccaa
    14281 ataccggttc tgtgtatgac ggtggcagaa caaccatttt caatgaaaac catccttttc
    14341 attttatttt ctgcataaca tttcttattg ggataatttt tattatatat caatcatata
    14401 atgataatta atttttaaca tccttactgt cctatcatga tgaaatgaca ataaggatgt
    14461 tttcctgctt tggctacggc aaaacaccgt cgtcattccc acgcaagcgg gaatctatat
    14521 gtttggtttc tcttttattt tcaaacgata atgaaacagg taagtccgga ttcccacctg
    14581 tgttggaatg acggaaatgt gcatttctaa tttttaccca ctataaaaac cccgaatcct
    14641 gattggcagg attcgggttt ttttatcgct gatgccgttc agacggcatt ttcaagcagc
    14701 ttattgatcc acaaacgcgc gctcaatcag gtagtcgccg cgcacgccgg ttttcgggga
    14761 gactgtcaga ccgaaatcgt ccaaaacttt gcaggtgtct ttcagcatcg cagggctgcc
    14821 gcacagcatg gcacggtcgt cttgggggtt gattttgggc aggccgatgt cttcaaacag
    14881 tttgccgctt accatcaggt cggtcaggcg gccgcggtgt tcgaattctt cgcgggaaac
    14941 aatcgggtag taaatcagtt tttctttaac caagtcgccg aggtattcgt gttcgggcaa
    15001 ttctttggta aagcggtcgt agtatgccaa atcctttttg tagcgcacgc cgtgtacaag
    15061 gatgattttt tcaaactgtt cgtaaatttc ggggtctttg gtgatgctca agaaaggggc
    15121 gatgccggta ccggtactca acaggtaaag gtgtttgccc ggattcagat cgcaggcaac
    15181 tagagttccg gtcggttttt tgctgattaa cacttcgtcg ccgactttga ggtgttgcag
    15241 gcggctggtc agcgggccgt cttggacttt aatgctgaaa aattcgaggt gttcttccca
    15301 gttggcggag gcgacgctgt atgcgcgcat cagcggcttg ccgtccgcca tcagcccgac
    15361 catgacgaac tgtccgtttt caaagcgcaa cgattcgtcg cggatgcagg taaaggtaaa
    15421 atatgcgtct gtccagtggt gtacggacaa tactttttgg gtattgaatg ctgccatttg
    15481 ggtttcctgt cagtaaaaga aatggatagt gcttgttcgg gacgtgcggc agagtggaaa
    15541 tgtctgcccg attcgggata aagtcaaaat tctaaacgaa acggatggtt gcgacaatgc
    15601 ctgatgcgcg ttggttatat gccgtctgaa gggcttcgga cggcatcgtg gggcgggcgg
    15661 tggcttacgg cggcatatcg gcgagggaaa ttaaggaaat cgaggctgcc agcctgagcg
    15721 cgtcccgtcc ccagcggggg tgttccagcc tgcgtatcgg gagaatcggt ttgacggtgc
    15781 cggcaatcag cttttgtatt tcggcaggct gatccgacaa tacgccgccc catttttccg
    15841 cagccgcttc cagcagtccg ccgtttttca atatcagcgc ggtcgaatgg atgtagcaga
    15901 gccaatcgcg ggcttggcat tgcgctatgg tcaggacctc ggaagggtcg tcttcaaaat
    15961 ccaaaaagct gatgtttttt ccgtcccaca tcatatttcg tgcaaacgcc tgactgagga
    16021 actgccgttt tttatgcacg cgtgcaatgg cttccaaacc ggcaagccaa gcgtccgcct
    16081 ttccggcttc ggcttcttgg cggatttgcg tatcgagcgg gatgccttcc aaattgccga
    16141 acataagggc atttttccgg agggcgagca attcgggaac ggctatccct gccgaacgca
    16201 attcgtacag gcgttttgat tcggttgcaa tggcaggctc gccgccgagg ctgggaaccg
    16261 gcttcaacac ccccagtttc aaatatcggg caaccatacc gagcagcgcg taacgccatc
    16321 gcgcattgtg cctgcctgct ttgcgtaccc ataccttggt cccgtcggca agcaggtagg
    16381 gggcgatggt tgcctcctgt tttgccgcca attcgtccag cagtatagaa aaacgggttt
    16441 cctgcatagg taaggtcatt ttctttcaat cttaagttcg gatggaatgc cgcccatacg
    16501 gaacatcagg caagggtttg ttcgatgatg ccttttaccg catccgcact gttcggcacg
    16561 gtttgcacgc gctgcggcag gttttccaaa ccttccagcg cggcggggcg cggaatggcg
    16621 acatcgccga cggcttcgcg tatggtcgca tcgaatttcg ccgccaacgc ggtttccaaa
    16681 caaaccaccg tttcccccgc ttcgcgcact tcgcgggcga cttttacgcc gttggcagta
    16741 tgcgggtcga tgagttcttt gtcttgctcg taaacctgtc tgatggtggc gaggcggtcg
    16801 gcgtgggtag atttgccgga ggtaaagccg tatttgccgc cgactttgtc caaggcaaac
    16861 cgcaggtcaa agcctttgcc tgccgcgact tccgcccaca gcgtattgat ttcctgagga
    16921 tcgcgatcca tcaggtcgaa cacgaaacgc tcgaagttgg acgctttgga aatgtccata
    16981 gacgggctgg aggttacata agtatgcgcg ctgttgcgcg ggcggtatgc gccggttttg
    17041 aaaaactcgt ccagcacatc gttttcattg gtcgcgacaa tcaggcagcg gacaggcagg
    17101 cccatttgtt tggcgatgtg tcccgcgcaa acgttgccga agttgccgct cggtacgcaa
    17161 aagctgacgg tttcgtcatt gcttgaagtg gcattgaaat agcctgcaaa gtaataaacc
    17221 acttgcgcga cgatgcgccc ccagttgatc gaattgaccg taccgatatg gtatttttcc
    17281 ttgaacgcgg catcgttctg cactgccttc acaatgtcct gacagtcgtc aaacatcccc
    17341 ttcacggcga tattgtggat attcccgtct tgcaggctgt acatctgcgc gcgttggaac
    17401 gcgctcattt taccgtcggg cgacaacata aatacgttca cgcctttttt gccgcgcaag
    17461 gcatattccg cagccgaacc cgtatcgccg ctggttgcgc ccaagatatt gagttttttg
    17521 ccttctttgt ttaaaacata ttcaaacgca ttgcccaaaa actgcatcgc catatctttg
    17581 aacgccagcg tcgggccgtt ggacaaggct tggattttga tgccgtctga aagcgtgcgg
    17641 acgggggtga tttccttagt accgaacgcc gcttccgtgt aagtacggtt cagaatgtcg
    17701 cgcaaatcgt cctccggaat atcggtaacg aacaggcaca tgatttcaaa cgccaattcg
    17761 ggataagcca aaccgcgcca tttgtccaag gtttcgcgcc cgatttgcgg ataatgttcc
    17821 ggcagcatca gtccgccgtc gggtgcaagc cccatcaata aaacttcgct gaacggtttg
    17881 tgtgcggttt cgccgcgcgt gctgatgtat ctcatgattt ttctcgtttg tcgaaattgc
    17941 aggaaaacgg attcagacgg catctgcctc atgccgtctg aagaaggtta gcggtacagg
    18001 cgtttgaagc aggcggaaac cgttttgacg gtcagggcgg caagtgcctg attgcgcgtg
    18061 gactggttca gcatctgcat cacatcgttg ccggtcagct ggttaggtgc ttcttcggta
    18121 gcgcagccgc aaatcttgtt ttcccattcc gcctgttttt cggcactcat cgccagcgcg
    18181 gtcaaacgcc attcgctgcg cctgtccaat tccgcacggc attggctttc taccgtcatt
    18241 ttgacgatgc tgccacccat acccgtgccg ccgtctaagc tgccgaatgt gttgccgccg
    18301 gcggcgcacc cgccgagtaa gattgccgcc ggcaaaatag acaaggtttt attcatctca
    18361 attccttttc ggttgaaacc ccgcctttta tggcgatagg atctgattag ccgccccgtt
    18421 cgggataacg cgaagggcgg cgttttatgc gccgttccga gtgttggagc aaaccgtttg
    18481 aatatccggt tgaagcccgg caacattata cttcaatcgg gaaaataaaa aatcccgccg
    18541 ccgtcatttt gcctgtttgc aaaaatgccg tctgaaaccg gttcagacgg catttgcgat
    18601 ttcagcctag cccaaagatt tgaactgctc caaaaacgga gggatgccgg gcagcatccc
    18661 gaccgcaccc atcgccacac acaagattaa gaaacctact gcgggtatca atactcgtcc
    18721 gacaaaacct aattgcgcgc tgcgttcttt gcagccgatt aagcccaaat tatccaacag
    18781 catcgtcaac gcccagccga aaaccggatt gaccagacgg aagagaacac cacgatggcg
    18841 gcggattggg tggttttgcc tttgcgcgtc atttccatgc ccgcttccaa aagcggtaag
    18901 tatacgccta cgatcaaggc tacgctcaat accggctgcc aaatcgccaa gtccatcgga
    18961 tagccccaca acccggcgat aatacataaa accgccgtta aaaccgcacc gcccggaatg
    19021 gggcgtttgg caatcgatgc cggcacgata taagttcccc aagaagaggt aaaatttgca
    19081 ccccctaaaa tagaacccac tgcttgacgg acagaacaac ttgtcatggt gtcgtctata
    19141 ttcatcaata ccttatcggt tttttccgga tagctgattt tttggaacac ttgatgtcct
    19201 aaaaaatcgg gcgaccacat tgcaacagcc aataccgcaa atggaaagac aaccaaaaaa
    19261 ctttctgccg tcggcaaccc cagatgccag ccgctgtttt caccctacca ataagcagga
    19321 ctcattggag gcaggccggg ggcggtgtga aactcaaacg gcgcacccaa tgcaaatgcc
    19381 gccacaccgg caatcaagca tcccaaaggc acggccaacc agcgtttttt ccaatgctcc
    19441 aacaaagcgt acatcacaat cgttacaata atgacggtaa aagcgatgta gggcatatta
    19501 aaaccgcctg cccacgaaaa caattttttt acctgccccg tcgtgccgat aaagcccaaa
    19561 tagagtaata atccgccgca tacgccgttg cttgtcagct tcgccataat actgccgccg
    19621 cgaaataaag ccatcagcag acctaaaacc gcaatcgaaa tgccgaacgc caaaggatgc
    19681 ccgcctgccg acacaacgat gggaatcatc ggaatcagcg gcccgtgcgt accgggcagg
    19741 ttggcgccgg gcagaaaaaa gcccgatacc aataagataa acgcggcggc gattaaaagc
    19801 tcatagcgca cattttccag tacaaagctg tcaggcagcc ccaaaggtgc
//
LOCUS       DAXFHF010000041        19536 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXFHF010000041 DAXFHF010000000
VERSION     DAXFHF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19536
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.041"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(154..477)
                     /locus_tag="ACFIRM_001333"
                     /pseudo
     CDS             complement(154..477)
                     /locus_tag="ACFIRM_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            481..804
                     /locus_tag="ACFIRM_001334"
                     /pseudo
     CDS             481..804
                     /locus_tag="ACFIRM_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(865..2919)
                     /gene="metG"
                     /locus_tag="ACFIRM_001335"
     CDS             complement(865..2919)
                     /gene="metG"
                     /locus_tag="ACFIRM_001335"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC3028952.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAAAAEGKLRLLDVDAGAQPGDK
                     VG"
     gene            complement(3035..4873)
                     /gene="glmS"
                     /locus_tag="ACFIRM_001336"
     CDS             complement(3035..4873)
                     /gene="glmS"
                     /locus_tag="ACFIRM_001336"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC3028953.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5151..5669
                     /locus_tag="ACFIRM_001337"
     CDS             5151..5669
                     /locus_tag="ACFIRM_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028954.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRRYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
     gene            complement(5952..7277)
                     /locus_tag="ACFIRM_001338"
     CDS             complement(5952..7277)
                     /locus_tag="ACFIRM_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC3028955.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPIHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTEQARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPHKLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7510..8328
                     /gene="efeU"
                     /locus_tag="ACFIRM_001339"
                     /pseudo
     CDS             7510..8328
                     /gene="efeU"
                     /locus_tag="ACFIRM_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            8325..9491
                     /gene="efeO"
                     /locus_tag="ACFIRM_001340"
     CDS             8325..9491
                     /gene="efeO"
                     /locus_tag="ACFIRM_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC3028956.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            9799..11064
                     /gene="efeB"
                     /locus_tag="ACFIRM_001341"
     CDS             9799..11064
                     /gene="efeB"
                     /locus_tag="ACFIRM_001341"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC3028957.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQTALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWGRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHMRLANPR
                     DPEFLKKHCLFRRAYSYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(11136..11465)
                     /locus_tag="ACFIRM_001342"
     CDS             complement(11136..11465)
                     /locus_tag="ACFIRM_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC3028958.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(11541..12911)
                     /gene="glmU"
                     /locus_tag="ACFIRM_001343"
     CDS             complement(11541..12911)
                     /gene="glmU"
                     /locus_tag="ACFIRM_001343"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC3028959.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(12966..13241)
                     /locus_tag="ACFIRM_001344"
     CDS             complement(12966..13241)
                     /locus_tag="ACFIRM_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC3028960.1"
                     /translation="MRKTFLILMTPAALFLSGCAWETYQDGNGKTAVRQKYPAGTPVY
                     YQDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(13234..13947)
                     /locus_tag="ACFIRM_001345"
     CDS             complement(13234..13947)
                     /locus_tag="ACFIRM_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC3028961.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHAPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTNPENTKECYA"
     gene            14006..15007
                     /locus_tag="ACFIRM_001346"
     CDS             14006..15007
                     /locus_tag="ACFIRM_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3028962.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSLEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            15101..15949
                     /locus_tag="ACFIRM_001347"
     CDS             15101..15949
                     /locus_tag="ACFIRM_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC3028963.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            15975..16460
                     /locus_tag="ACFIRM_001348"
     CDS             15975..16460
                     /locus_tag="ACFIRM_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC3028964.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDA
                     V"
     gene            complement(16544..18112)
                     /gene="msrAB"
                     /locus_tag="ACFIRM_001349"
     CDS             complement(16544..18112)
                     /gene="msrAB"
                     /locus_tag="ACFIRM_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC3028965.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGEVK"
     gene            18258..19511
                     /gene="ftsY"
                     /locus_tag="ACFIRM_001350"
     CDS             18258..19511
                     /gene="ftsY"
                     /locus_tag="ACFIRM_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC3028966.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRTESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQATGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4021 a         5006 c         5978 g         4531 t
ORIGIN      
        1 ggcagatgat ttatcgcggc aggttgacgg caggtgcttg gtgtcgattt ctttgtcttt
       61 gccggtggtg tcggcggtga cggcgtcggt gccggcgttg tcgcgcgtaa ccggctgtcc
      121 gcagaaccat tttaccgaac cgatttgaaa gcaacggcag gttgacggca ggtgcttggt
      181 gtcgattttg ccgttggtgt cggtgtcggc ggtgacggtg tcgtcgttgg cggcgttggc
      241 gttgtcgcgc gtaaccggct gtccgcagaa ccattttacc gaaccggctt cacgcttggc
      301 ccacagggag agttttttgt ttttgatttc attgtttacg ccgcttgaaa gcattttggc
      361 ggtaacgacg cctttttcga ccgtaacgct ttcaacatat ttgcctttga tttctgaagc
      421 ggatgccacg ccggcatcgc cgttgtttgc gggccattcg ccgtgattca ggtaataccc
      481 ggcgaaaacg aattgcggca gatgaagccg ggtgcggttt tgatcaattg cgggcgcggc
      541 gggctggcgg atgaaaacgc gctgcttgcc gcactcaaat acgggcagat cggcggggcg
      601 ggtgtcgatg ttttgacgga ggaaccgccg aggggcggca atcccctgtt gaaggcacga
      661 cttcccaatc tgattgttac gccgcatacc gcgtgggcaa gccgcgaggc tttggacagg
      721 ttgtttgaaa tattgttggc gaacattcac gcctttgtga aaggcgaggc gcaaaaccgg
      781 gtggtttggg cttgtcggaa ttaaggaaac aatgccgtcc gaacgcccaa agggttcgga
      841 cggcattgtt tttgatattt ctgcttaacc gactttgtcg cccggttgcg cgcctgcgtc
      901 gacatcaagc agccttagtt tgccctctgc cgcggcggca ctcaaaatca taccttcgga
      961 tacgccgaat tttgccattt tgcgtggggc gaagttggcg actgcgatga ccatgcgacc
     1021 gttcaattcg gcaggatttg ggtaagacgc ggcgatgccg gagaagatga tgcgtttttc
     1081 aaaaccgaaa tcgaggtcga atttcaaaag tttggtgctg ccttcgacgg cttcgcagtt
     1141 caatactttg gcgacgcgca tatcgatttt cataaagtcg tcgaagcttg cctgttcggc
     1201 gactttttcg tatttaccct cttcaacggg cgcggatgcc gtctgaatgc tttgtttgtt
     1261 ggcttcgatc aaatcgtcca cttgtttttg ctccactcgt tgcattaaat gttcgtattt
     1321 gttgatggcg tgttcgccta gggtttcgcg cgtgttcttc caggtaatcg cgtccagatt
     1381 gaggaaacgc gcggcgttgg cggcggtttg cggcaacacg ggggcgaggt aggcggtcag
     1441 catggtgaag gcgttgatga gttcgctgca tacttcgtgc aggcgttcgt cttgaccttc
     1501 ttgtttggcg agctcccacg gtttgttggc atcgacgtat tcgttgacaa tgtctgccaa
     1561 tgccatgatg tcgcgcaggg cgcgggcgta ttcgcggttt tcgtattgct cggcgatggt
     1621 gtcgctttcg gcggcgagtt ttgccagcaa tgcgctgcct gaaacgtctt tcggacgacc
     1681 ttcaaagcgt ttggcgatga aacccgaagc gcgggcggcg atgttgacgt atttgccgac
     1741 aaggtcgctg ttgacgcgcg aaataaagtc ttgcaggttc aaatcgatgt cttcgatttt
     1801 gctgttgagc ttggcggcga tgtagtagcg catccactcg gggttcagac cttgttccaa
     1861 ataggatttg gcggtgatga aggtgccgcg cgatttggac attttttgtc cgtccacggt
     1921 caaaaagccg tgtgcgtaca cgccggtcgg ggcgcggtgg ccggagaaat gcagcatggc
     1981 cggccagaac agggcgtgga aatagaggat gtctttgccg atgaagtggt acatctcggt
     2041 ttggctgccg gctttgaagt attcgtcaaa atcgatgccg atgcggtcgc acaggttttt
     2101 aaacgatgcc atgtagccga cgggcgcgtc gagccagacg tagaagtatt tgcccggcgc
     2161 gtcggggatt tcaaaaccga aatacggcgc gtcgcgggaa atgtcccagt cggacagggt
     2221 ggtttctccg ccttcgccca accattcctt cattttgttg agggcttccg cctgcagatg
     2281 gggcttgccg tcgtgcgggt tgttgccgga agtccatgct ttgaggaagt cggcgcattc
     2341 gcccagtttg aagaagaagt gttcggattc gcgcaattcg ggtttcgcgc cggaaacggc
     2401 ggaatacggg ttaatcagtt cggtcgggga ataggtcgtg ccgcagactt cgcagttgtc
     2461 gccgtattgg tcttgggcgt ggcatttcgg gcattcgcct ttgacgaagc ggtcgggcag
     2521 gaacatttgt ttttcggggt cgaaaagctg ctcgatgacg cggctttcga ttttgccgtt
     2581 ggctttcagc gcgcggtaaa tgtcttggga aaactgtttg ttttcagtgg aatgggtgct
     2641 gtaataattg tcgtagccga tgaaaaagcc ggtaaagtcg gcaaggtgct ctttgcgcac
     2701 tttggcaatc atgtcttcgg gcgcgatgcc ttgtttttgc gcggcaagca taacgggcgt
     2761 gccgtgggtg tcgtcggcgc agcagtagta gcattcgtgg ccgcgcagtt tttgaaagcg
     2821 cacccaaacg tcggtttgga tgtgttcgac catgtggccg aggtggatgc tgccgttggc
     2881 atagggcagg gcggaggtaa ctaagatttt gcgtgtcata ttgtgctttg caaacaatgg
     2941 gtaaaggcgg attataccgc aaatcaaacg gggaaatgcc gtctgaagcc tgaaaaatcg
     3001 ggcttcagac ggcatttttg ccaaccggcg ggatttattc gacggttacg gatttcgcca
     3061 ggttgcgcgg cttgtccaca tccgtgccgc gcgcgagggc ggtgtggtag gcgaggagct
     3121 gcacggggac ggtatgcacg acgggggaca gttcgccgac gtggcgaggt gcgcggataa
     3181 cgtgcacgcc ttcggcggca ttaaaattgc tgtcgaggtc ggcaaagacg aaaagttcgc
     3241 cgccgcgcgc gccgacttcc tgcatattgg ctttgacttt gtccaacagg ctgtcgttgg
     3301 gcgcgatgac gacgacgggc atgttttcgt ccaccaaggc aagcggcccg tgtttcagtt
     3361 cgccggcagg ataggcttcg gcgtggatgt aggtgatttc ctttagcttc aacgcacctt
     3421 caagggcaat cgggtaatgg atgccgcgcc ccaaaaacag cgcgctggtt ttcttggcaa
     3481 actgttgcgc ccaagcggca atttgcggtt cgaggttcaa agcgtgctgc acgctgccgg
     3541 gaagctggcg gagttcttcg gtgtaacgtg cttcatcttc gccggatacc acgccgcgca
     3601 ctttcgccag cgttaccgcc aaaccgaaca gcacgaccag ttgcgtggta aatgctttag
     3661 tcgaggcgac gccgatttcc gcaccggcac gggtataaag cacaaggctg ctttcgcgcg
     3721 gcagggcgga ttccatcacg ttgcagacgg agaggctgtg gcggtgtccc aaagatttgg
     3781 cgtatttcaa agcctccatc gtgtccagcg tttcgccgga ttgggaaatg gtaatgacca
     3841 gttggtcggg gtcggcaatc acgctgcggt accggtattc gcttgcgatt tcgacgtcgg
     3901 ccgggatttt ggcgatggat tccaaccaat atttggcggt cagcgcggcg taataggacg
     3961 tgccgcaggc aaggattttg acgctgcgga tgctttcaaa cacgcttttg gcatttttgc
     4021 cgaagttttc ggggatgaat ccgccgtcga ggaaaacttc cgccgtgtcc gcaatcgcgc
     4081 gcggctgctc gtggatttct ttttgcataa agtggctgtg cggacccggt tccaaagagg
     4141 cgggcgagag ttcggatacc ttgactttgc gttcggcagg caggccgttt ttatcggtca
     4201 gccgtttgat gccgtctgaa gccagcagcg cgatgtcgcc gtcttcgagg tacgccacgc
     4261 ggcgcgtaaa ggcgatgacg gcggatacgt ccgaagcgat aaaggtttca tcgtcgccca
     4321 aagcgaccaa aagcgggcag cccatacgcg ccacaactaa ttcatcaggc ttgtcttggg
     4381 caataaccgc gatggcgtat gcgccgtgga aacgtttgac cgcttcctgc acggcttcaa
     4441 acagcctgcc gccgttttgc gcgtattcgt ggttgatgct gtgtgcgatg acttcggtat
     4501 ccgtttgcga ttcaaaccgg tatcccaaac cttccaagcg tttgcgttcg ctttcaaagt
     4561 tttcgatgat gccgttgtgg acgacggcaa tcataccgcc gctgatgtgc gggtgggcgt
     4621 tcggctcggt aacgccgccg tgtgtcgccc agcgcgtatg cccgatgccg atgccgccgc
     4681 tgatgccttt ttcgcgtgcc gcgtcctcca taagctgcac gcgtccgacg cggcgcacgc
     4741 gtttgatttt gccgtcggtg ttgacggcaa tgcctgatga gtcataaccc cggtattcga
     4801 ggcgtttgag gccgtcggtc agaaaatcga cgacgttgtg atgggcgcgg atggcgccga
     4861 cgataccgca cataaactgt tccttagtat ccggttgaaa aaaaagatgc ggacggcttc
     4921 cgtaccgcac cttccgtttc ggattataaa ccgcctcccg cgccggaaaa cagcaaaatg
     4981 ccgtctgaag gcttgggctt gctcaaaaaa gggagggatt tccccgcctg accgggatgg
     5041 gcgttcagac ggcattgcct gccgccggtt tatagttttt gcaaatcaat attaacaagc
     5101 tgaaaaaaaa caatatgctc gcccggtctt aatgttaacg gagtatggaa atgaaacaaa
     5161 tgcttttggc cgtcggcgtg gcggcggtgt tggcgggctg cggcaaggat gccggcggtt
     5221 acgagggtta ttggcgcgaa aagtcggaca aaaaagaggg cgtgattgcc gtcaaaaaag
     5281 aaaaaggcaa ttacttcctt aataaaatca acgtgtttac aggcaaggag gagtctttgc
     5341 ttttgtctga aaaagacggc gcgctttcga taaacacggg gataggggaa atcccgatca
     5401 aactttccga cgacgggaaa gagctgtatg tcgaacgcag gcggtatgtg aaaaccgatg
     5461 cggcgatgaa ggacaaaatc atcgcccacc agaaaaagtg cggacaaacg gcacaggcat
     5521 acctcgacgc gcgaaatgcg ttgccgtcaa accaaacgta tcagcagcgt caggcggcga
     5581 tcgagcaatt gaaacggcgg tttgaagccg agtttgacga attggaaaaa gaaatcaaat
     5641 gcaacggcaa accgacattg ttgttttagc gtaggacaac cgggaggatg ccgccgtccg
     5701 aatcggacgc gtggtttcca taccggtacg ggcgggcggg aatgtccgcc tttttttgtt
     5761 cggatgcgtt tgaatacccg tccgattctg tccgtttgca agggggattt ccgttcgggc
     5821 ggaaatccat tccgtcaaat aagaaaggcg ttttgtccga ccggccggct tgtgcctgcg
     5881 gtatcgcaaa acttcgcctt aatgcgcccg attggctagg gacgggcttc ggacggcatt
     5941 gttttgccgg ttcacgggcg gtattcgggc ttcatgccgt tgggcaggag ctgccagacg
     6001 tatcctgtgg ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa
     6061 taatccacgc gcaccgcgcc tttgatcgcg ctgcctgtat cctgcgccat aatcaggcgg
     6121 ttgagggctt tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta
     6181 atgtagtgcc ggtcgattgc gccggcgtat tcccccatca gcggcgtgcc cagtgcgccg
     6241 acggggccct cattgccgct tccggcaagc tcgcggaaaa agatatagct ggggttttga
     6301 cccaaaactt cggcgagttt gtgcgggttt tgccgcatat atgacttaat gccctgcatg
     6361 gaggtttgcc cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacatac
     6421 ggatgctcgt ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga
     6481 cggcccgagc cttgtatgtg catgaaaaaa agttcgacgg ggtcttcggc gtaaccgagt
     6541 atcggggctt tgccgtcaag cgcgccgccg ttgatttggt ttcgcgtgtg gtaggggagg
     6601 aagcggcttc cttcaaacct gcccttgatt gccgttgtgc gcgcggtaat ggggaatcgg
     6661 gaaaggtcgg cggtatgcgt gccgccggca ttgtcgattg tgccgctgtt ttttcccgtc
     6721 tgcctgatgc ggacaagggc tttcccgccc cgcaaaccgg caggcagggg gacggagata
     6781 aaatcgtcgg gaatgccgta aatcgggaag cgggcctgtt ccgtccgcct gtcgtcgccc
     6841 ttcagcaccg gctcgtaata gccggtaacc gtacctgcaa ggcttccgtt gcctgcaacc
     6901 tgccaaggcg tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gatgggggtt
     6961 tgaaaggctt gggcgcacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg
     7021 aggcggaagg attgcaggct tttggcaaaa tcctgcgccg cccagtgggg catggacagg
     7081 tgcggcacaa cggtatagac ggccccgccg ccggcaaccg tcgttccggc ggggtcgggg
     7141 atgccggccg gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg
     7201 atgctcctgc tttggcaggc ggcgaggatg gcggcggcga tgccgtacag ggcggagcgg
     7261 agcaggtgtt ttttcataat ggacaatgtt gtcgtcagta ataaaaaaag atggtttcgg
     7321 gcggcgttgc ggtagccggg gagaggggat tttaacacag ggcgcagccg cagcctgcgg
     7381 aactttccgc cacgcggcaa tgcagataaa aataacttgc atttgtattt gcaagtaatg
     7441 aaaatattcc gataatatat tattaatcat ccttgttcat tcgcgtttac gctggtcgct
     7501 tttttaatta tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt
     7561 tttttgaaac agtccggaca ttcaaaaact gatgcctaag gtctggttcg gggtcgtcct
     7621 tgcttctttg atgtgtttgg gcatcggata cggcatccat tcggcaacgg gcgagattcc
     7681 ccggaagcag caggagttcg tcgtcggcat tatcggtttg gttgccgtcg cgatgctgac
     7741 ttgtgtggtt ttatggatga aaaaggcggc gcgttcgatg aagcggcagc ttcgggattc
     7801 tgtacaggcg gctttgaacc gtggcagcgg tcaaggatgg ccttggtcgg tatggcgttt
     7861 cttgccgtgg cgcgcgaagg tttggagagc gtttttttcc tgcttgccgt attcaaacag
     7921 agcccgacgt ggcagatgcc ggccggcgcg gtagcggggg ttttggctgc cgccgtgatt
     7981 ggcgcgttga tttatcaggg cgggatgcgc ctgaatctgg cgaagttttt ccgttggacg
     8041 ggggcgtttc tgattatcgt tgccgccggt ctgcttgccg gctcgctgcg tgcgctgcat
     8101 gaggcaggca tttggaacgc gcttcaggac attgtgttgg acccatcaaa atatttgcac
     8161 gaagacagtc cgttgggtgt gctgctcggc ggatttttcg gctataccga ccatccggcg
     8221 cagggcgggg ccttggtttg gctgctgtac cttattcccg tcatgatttg gtttttgcgc
     8281 ggcagcaggc cgtctgaaac tttaacccgt aaagaggaat tgaaatgaga aaattcaatt
     8341 tgactgcatt gtccgtgatg cttgccttgg gtttgaccgc gtgccagccg ccggaggcgg
     8401 agaaagccgc gccggccgcg tccggtgaga cccaatccgc caacgaaggc ggttcggtcg
     8461 gtatcgccgt caacgacaat gcctgcgaac cgatgaatct gaccgtgccg agcggacagg
     8521 ttgtgttcaa tattaaaaac aacagcggcc gcaagctcga atgggaaatc ctgaagggcg
     8581 tgatggtggt ggacgaacgc gaaaatatcg ccccggggct ttccgacaaa atgaccgtaa
     8641 ccctgctgcc gggggaatac gaaatgacct gcggcctttt gaccaatccg cgcggcaagc
     8701 tggtggtagc cgacagcggc tttaaagaca ccgccaacga agcggatttg gaaaaactgc
     8761 cccaaccgct cgccgactat aaagcctacg ttcaaggcga ggttaaagag ctggcggcga
     8821 aaaccaaaac ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt
     8881 ttgccgacac ccgcgtccat tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac
     8941 tcgaccccgt catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa gatgccgggt
     9001 ttaccggctt ccaccgtatc gaacacgccc tttgggtgga aaaagacgta tccggagtga
     9061 aggaaaccgc ggccaaactg atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat
     9121 tggcgttccc tccgggcaaa gtggtcggcg gcgcgtccga actgattgaa gaagcggcgg
     9181 gcagtaaaat cagcggcgaa gaagaccgtt acagccacac cgatttgagc gacttccaag
     9241 ctaatgcgga cggatctaaa aaaatcgtcg atttgttccg tccgttgatt gaggccaaaa
     9301 acaaagcctt gttggaaaaa accgatacca acttcaaaca ggtcaacgaa attctggcga
     9361 aataccgcac caaagacggt tttgaaacct acgacaagct gagcgaagcc gaccgcaaag
     9421 cattacaggc tcctattaac gcgcttgccg aagaccttgc ccaacttcgc ggcatactcg
     9481 gcttgaaata agccgcaagc gttcagacgg tatttggcgg cagataccgt ctgaagtttt
     9541 atagcggatt aacaaaaacc ggtacggcgt tgccccgccc cggctcaaag ggaacggttc
     9601 cctaaggcgc ccaagcaccg ggcgaaccgg ttccgtacca tttgtactgc ctgcggcccg
     9661 ccgccttgtc ctgatttttg ttaatccgct atatccgcca tatgttgcag ggcgggcttt
     9721 cagaccgccg ctatcggtta atggaaaaac ggcgtgcagg gatgcccacc ctgctgcacg
     9781 gatattgaag gaaacgccat gagtcaaaac caacccgcac aaccgaccaa acgcaatctg
     9841 ttcaaaaccg cccttgccgt cggcgcaatc ggcgcaatcg gaggttattt cggcggcaaa
     9901 aaacagggcg aaaccgccga acgcaccgcc gaaagccaac actcgcccca agcctatccc
     9961 tgctacggcg aacatcaggc aggtatcgtt acgccgcggc aggcgttttc cattatgtgc
    10021 gccttcgacg taaccgcgca aagtgccaag cagctggaaa acctgttccg cacactgacc
    10081 gcccgcatcg agtttctcac ccaaggcgga gaataccaag acggcgacga caaactcccg
    10141 tcagccggca gcggcatttt gggcaaagcc ttcaaccccg acggattgac cgttaccgtg
    10201 ggggtgggca gcagcctgtt tgacggccgg ttcggactca aagacaaaaa aacggttcat
    10261 ttgcaggaaa tgcgcgactt ccccaacgat aagctgcaaa aaagctggtg cgacggcgat
    10321 ttgagcctgc aaatctgcgc cttcaccccc gaaacctgcc aaaccgccct gcgcgacatc
    10381 atcaaacaca ccgcccaaac cgccgtcatc cgctggagta tcgacgggtg gcagcctaaa
    10441 tccgaacccg gcgcgatggc ggcgcgcaac ctgttgggct tccgagacgg cacgggcaac
    10501 cccaaggttt ccgatcccaa aaccgccgac gaggttttat ggacgggcgt ggccgccaac
    10561 agcctcgacg aaccggagtg ggcgaaaaac ggcagctatc aggcagtccg ccttatccgc
    10621 cgctttgtcg agttttgggg caggacgccg cttcaagagc aaaccgacat tttcgggcgg
    10681 cgaaaataca gcggggcgcc gatggacggc aaaaaagaag ccgaccaacc ggatttcgcc
    10741 aaagaccccg agggtaatac cacgcccaaa gacagccata tgcgcctggc gaatccgcgc
    10801 gatcccgaat tcctcaaaaa acactgcctc ttccgccgcg cctacagcta ttctcgcgga
    10861 cccgcctcaa gcggacagct tgatgtcggg ctggtgttcg tctgctatca ggcaaacctt
    10921 gccgacggct tcatcttcgt gcaaaacctc ctcaacggcg aaccgctgga agaatacatc
    10981 agccccttcg gcggcggcta tttcttcgtc ttgcccggcg tgggaaaagg cggattcttg
    11041 ggacaagggc tgccgggcgt ataaacctgc ctgacggaca aatgccgtcc gaaccttgcc
    11101 aaacgggttc ggacggcatt ttactgtttg gacggtcagg cttttttgac gaattcggat
    11161 tttaaattca tcgcgctgcc gtcgattttg cagccgatgt tgtgatcgcc ttcctgcagg
    11221 cgtatgcctt tgaccttcgt accttgtttg atcgtcatcg agctgccttt taccttgagg
    11281 tctttgatga ggatgacggt gtcgccgttt tgcagcgccg cgccgttggc atcgcgcact
    11341 tgagccgcaa ggtcggcggc ggattcggtt tcattccatt cgtgggcgca ttcggggcag
    11401 atgtattgtc cgccgtcttc ataggtgtat tcggaggcgc attgcgggca agggggtaat
    11461 gacatggttt gccgtcctta tcggatgttt gttttgggat gccgtctgaa acctgaaacc
    11521 ggcttcagac ggcatggctt ttattgtttg tctttttccg gccttaccca gccttcgatg
    11581 acggtttggc gggcgcgggc gagggcgagt ttgttgtctt cgatattgcg ggtaatcgcg
    11641 ctgcctgcgc ctgtggttac tttgttgccc agtgttacag gggcaaccag tacgcagttc
    11701 gaaccgatgc gcacttcgtc gccgatgacg gttttgtgtt tgtgcacgcc gtcgtagttg
    11761 gcaataatcg tgcccgcgcc gaagttggtt ttgctgccga cttcggcatc gcctatgtag
    11821 gtgaggtggt tggctttggt gcctttgccg atggcggcgt ttttgatttc gacgaagttg
    11881 ccgacgtgta cattgtcagc cagtttggct tgcggacgca aacgggcgta cgggccgatt
    11941 cggttgtttt cgccgacttc gcagccttcg aggtgggaga agggggcgat tttgctgttt
    12001 gcgccgattt tggcgttttt gatgacgcag tttgcgccga tttcgacgtt gtcgccgagt
    12061 tcgacttcac cttcgatgac gacgttgacg tcaatcacga catcctgtcc gtgtttcaga
    12121 cggccccgca aatcgaaacg agccggatcg cgcagggtca cgcctgcttt gagcaattct
    12181 tgcgcctgtt cggtttggaa gatgcgttcg agttcggcga gctggcgttt gttgttcacg
    12241 ccggcggcga ggtgggaggc gcgcacccgg acggggcgga ctttgatgcc gtcggcaacg
    12301 gctttggcga tgaggtcggt taaataatat tcgccttggg cattgttgct ggaaaggctg
    12361 ttcagccagt tttccagttt ggcgttgggc aggacgagga tgcctgtgtt gatttcgcgg
    12421 atggtttttt gggtggcact tgcgtctttt tcttcgacaa tggcggttac gctgccgctg
    12481 ccgtcgcgga tgatgcgccc caagcctgtc gggtcggcgg gcacgtcggt caacagcccg
    12541 acttcgttgc ctgcggcttc gagcagggtt tcgagggttt caacgtcaat taaaggaacg
    12601 tcgccgtaca acaccagcgt gcggccttcg gaggcaaggt ggggcagggc ggttttgacg
    12661 gcgtggccgg tgccgagttg ttcggtttgt tcgacccaga cggcatcgcg tttgacagtg
    12721 tctaaaactt gctctttgcc gtgtccgacg acgacgcaga tgttttgcgg attcagcgcg
    12781 gcggcggtgt cgataacgcg ctcgaccatg ggcttgccgc cgatgcagtg cagcactttg
    12841 ggcatttggg aatacatgcg cgtgcctttg ccggcggcga ggatgacggt gtttaaagtg
    12901 ttttgcggca tagcgttttc ctgtgccatg ccgtctgaag cggtttcaga cggcataggg
    12961 taggtttatc ggttttgaaa ctttggtttt tgccagtgtt ggcgatgctc ttcgtcggcg
    13021 ttgttgccgg tttggtcggg cagcacggcg cggcgttcgg gacggtattg gttgtagttc
    13081 atatttttcg agtagctgcc gtcttggtaa taaacgggcg tgccggcggg atatttttga
    13141 cggacggcgg ttttgccgtt gccgtcttga taagtttccc acgcgcagcc cgacaaaaaa
    13201 agggcggcgg gggtcatcag gataaggaag gttttacgca taacattctt tcgtattttc
    13261 ggggtttgtg cgggtattgt aatgattttg gcggcgttct gacaaagttt ctgcataccg
    13321 tgccagttgc gccatatcgc ttacggaggc atcgataaag ggcggcgcgt gggattttgc
    13381 accgaaccgg acggttttca tgcccaatgc ttttgcctga tggaggttgt ccgcgctgtc
    13441 gtccaccata atgcagcatt cgggcggtac gtccaacagg cggcagacat tgagatacgc
    13501 ttgcggattg ggtttgtaca gcagtccgaa atcatccgtg ccgaacagtg cgtcgaaaca
    13561 gttttccaaa ccgagtgcgc cggcaacggc acggacgtaa aacgacgggc cgttggaaaa
    13621 aaccgccttg cgcccgttca gacggcatag ggtgttttcc gtatccgcca tgccgtgcag
    13681 cttggtcagg attgcatcga tcggatggct ttcgcgtaaa aattccgcaa tgtcgatttc
    13741 gggatggtgg atttgcagtc cggcgagcgt tgcgccgtaa cggtgccaat agtcttgacg
    13801 caggtcggac gcggcggatt cggagagttt gaggcggcgt gccatatagc gcgtcatagc
    13861 gcggttgatg aggtggaaga tgcctgcgtc ggcatcgtgc agcgtgttgt cgaggtcgaa
    13921 cagccacacg gtcgggtttt cttgcatttt gaaccgtgaa aatttgttag aatgttattt
    13981 tacagcgaat aaaggaggac tcggaatgaa acggaaaatt tggctgctgc cgctgctggc
    14041 ggtttcggca tacctgcagg cgcagacgga agtcaggctg gcggtgcata agtcgttcag
    14101 cctgcccaaa gggctgattg cgcgcttcga gcgggcaaac gatgcgaaag tgtcgattat
    14161 tcaggcgggc ggcgcgaacg aaatgctcaa caaactgatt ttgagccgag ccaacccgat
    14221 tgccgacgcg gtgtacggtt tggacaacgc caatatcggc aaggcgcggg aaatgggcat
    14281 tttggcggcg gcgcaacccg aatccgcccc cgtcgcggtc gggctgcctt cggctttggc
    14341 ggtcgattac ggctatgtgt ccgtcaatta cgacaaaaaa tggtttgaag gcaaaaagct
    14401 gcccctgccg caaaccctgc aggatttgac ccgccccgaa tataaaaacc tattggtcgt
    14461 gccgtccccc gccacatctt cccccggctt gggcttcctg atggcgaaca tcggcggtct
    14521 gggcgaagaa ggcgcgttta aatggtgggc acagatgcgg cagaacggcg tgaaggtcgc
    14581 caaaggctgg agcgaggcgt attacaccga cttttcgcac aacggcggcg cgtatccgct
    14641 ggtggtcggt tatgccgcca gccctgcggc ggaagtatat ttttccaaag gcaaatacag
    14701 cgagccgccg acgggcaacc tgtttttaaa aggcggcgta ttccgtcagg tcgaaggcgc
    14761 ggcggtcttg aagggcgcga aacagccgaa gctggcggca aaactggtgc aatggctgca
    14821 aagtctggaa gtgcagcagg cggttccgtc tgaaatgtgg gtttaccccg ccgtcaaaaa
    14881 cacgcgcctg cccgacgtgt tccgcttcgc ccaagccccg gcgcacacta ccgcccccgc
    14941 gcagcgcgat attgatgcga accagcgcgg atgggtttcc cgttggatca ggacggtttt
    15001 gaaataaaac aaacataccg ccccgcagag cttcagacgg catttttaca cctgtgccga
    15061 ttacgccgca cggggcggat gttcgatcga gaggaaaaca atggacttca aacaatttga
    15121 ttttttacac ctgatcagtg tttccggttg ggggcatctg gctgaaaagg cgtgggcgtt
    15181 cgggctgaac cttgccgccg cgctgcttat tttcttggtc gggaaatggg cggcgaaacg
    15241 cattgtcgcc gtgatgaggg cggcgatgac gcgcgcgcag gtcgatgcca cgctgattag
    15301 ttttttgtgt aatgttgcca atatcggctt attgattttg gtgattattg ccgcattggg
    15361 cagattgggc gtttccacaa catccgtaac cgccttaatc ggcggcgcgg gtttggcggt
    15421 ggcgttgtcc ctgaaagacc agctgtccaa ttttgccgcc ggcgcgctga ttatcctgtt
    15481 ccgcccgttc aaagtcggcg actttatccg tgtcggcggt tttgaaggat atgtccggga
    15541 aatcaaaatg gtgcagactt ctttgcggac gaccgacaac gaagaagtcg tgctgcccaa
    15601 cagcgtggtg atgggcaaca gcatcgtcaa ccgttccagc ctgccgcttt gccgcgccca
    15661 agtgatagtc ggcgtcgatt acaactgcga tttgaaagtg gcgaaagagg cggtgttgaa
    15721 agccgccgcc gaacacccct tgagcgttca aaacgaagag cggcagcccg ccgcctacat
    15781 caccgccttg ggcgacaatg ccatcgaaat cacattatgg gcttgggcaa acgaagcaga
    15841 ccgctggacg ctgcaatgcg acttgaacga acaagtggtc gaaaacctcc gcaaagtcaa
    15901 tatcaacatc ccgttcccgc aacgcgacat acacatcatc aattcttaaa cgccgtctga
    15961 aaggggggtg ggaaatggat gcgttgcaca ccatcgcccg aaacctgacg aaaaaacgtc
    16021 aaaccgtaag ctgtgccgaa tcctgcacgg gcggaatgct tgccgccgca ttcacaagcg
    16081 ttgcaggcag ttcgcaatgg ttcgaccaga gttttgtaac atacagcaac aaagccaaag
    16141 aataccgctt gggcgtgttg cccgaaaccc tgctcgaaca cggcgcggtc agccgccaaa
    16201 ccgtctatga gatggcgcgc ggcgcgaaag ccgtggctca ggcggattac gccgtcggta
    16261 tttccggcat cgccggcccg ggcggcggcg gcgaaagcaa acccgtcggc acggtttggt
    16321 tcgggtttgc ctttccgggc ggaagttgcg aagcaatgcg ccgttttgac ggcaaccgcg
    16381 aatccgtccg cgcgcaggcg gtcgccttcg cgttggaacg gttggccggg ctggttgaaa
    16441 acggcggcga tgctgtctga acaaatcccc gccggataaa aaatgccgtc tgaaacgttg
    16501 caggtttcag acggcatttt gccgggttag gcggcggtgc ggcttatttc acttcgccct
    16561 tcaacgcgcc gtagcctgcc gcgtccattt gttccagcgg gatgaatttc aagctcgcgc
    16621 cgttgatgca gtagcgcagt ccgcctttgt cgcgggggcc gtcggggaag acgtgtccca
    16681 agtgcgaatc ggcggcgcgg cttctgactt cggtgcggcg catattgaag ctgaaatcat
    16741 cgtgttcggt aacggatttt gcatcaatcg ggcgcgtgaa gctcggccag ccgcagccgg
    16801 aatcatattt gtcggcggag ctgaacaggg gttcgccgct gacaacgtcc acataaatgc
    16861 cgggtttgaa caaatggtcg tattcgtggc tgaaggcgta ttcggtcgcg ctgttttggg
    16921 tcacttggta ttgctcttcg gtcagggtgc gtttgagttc ggcgtcactc ggttttttat
    16981 acgttgccgc gtcgaagcct ttgccttgcg gtgcggcttt ggttttgccc ggcagcggtt
    17041 cgtcggcttt gcggatgtcg atgtggcagt agccgttggg gtttttaatc aggtagtcct
    17101 gatggtattc ctcggcgtcg tagaagtttt tcagcggttc gttttcaaca acgaggggca
    17161 gttggtattt ttgctgctcg cgtttgaggg cggcggcgat gacggctttt tcggcggggt
    17221 cggtgtagta cacgccgctg cggtattgcg tgccggtgtc gttaccctgt ttgttgaggc
    17281 tggtcggatc aacgacgcgg aaataatatt gcaggatgtc gtccaggctg agtttgtcgg
    17341 catcgtaggt cactttgacg gtctcggcat ggcccgtatg gcggtaggac acgtcttcgt
    17401 agctcgggtt ttccgtgttg ccgttggcgt agccggatac cgcgtcaacc acgccgtcga
    17461 tgcgttggaa ataggcttcc aagccccaga agcagccgcc ggcgaggtag atggtgcgcg
    17521 tgttcatgat tgctgaatcc tttttctgag tgtcgggttt gtagaacgaa tgtttcaaac
    17581 tgcccaaatc ggcattcggg ttgcggatta acgccaatgc ctgcgcttcg ttgatgctgc
    17641 ctttgacgat gcgctgcacg tcgccgtctt taccgattaa cgcccaagaa ggataaacgc
    17701 tgatattcag gttttgggcg atcgtgccgc cgttgtcggt aacgacgggc agcttgggat
    17761 agttcaaacc ggcataccat ttttgaaaat cgccgtcttt tttctcgtgc aaaaagcccg
    17821 gggaggcgac ggtaatcagg ttggcggagc tgaattttgc atcttgcgcc catttctcgg
    17881 cctgtcccaa ttcggacaaa cataaaggac accagctcgc ccaaaattta atcagcgtcg
    17941 gtttgtcttt tttcaaataa acactggcgg ggcggttgtc cgcggttttt aacgtggata
    18001 aagtgtgcgg cacggtcgcg gccccggcat cgacgatttt gggcgaacaa gcgcccagcg
    18061 caagcaggca gccgaacttg gcgcaaaggg aaaagaaagt acggtgtttc attttgatgt
    18121 ttcctgtgtg gacggtttgc atgattagac gtttgagatg ccgaaacctt acggcccgga
    18181 ttttcagaca accttgccgc gcaaaatacg ctacaatacg ccctatttca agtttctaaa
    18241 attaaaagga aaattcaatg ttcagcttct tccgtcgcaa gaaaaaacag gaaacgccgg
    18301 ctctcgagga ggcccaagtt caggaaaccg cagcaaaagt agaatctgaa gttgctcaaa
    18361 tagttggaaa tattaaagaa gatgtcgaat ctttagcaga aagcgtcaaa gggcggaccg
    18421 aatctgccgt tgaaaccgtc agcggtgcgg ttgaacaggt aaaggaaacc gttgccgaga
    18481 tgccgtctga agcaggggaa gcggcggaac gcgttgaatc cgcaaaagaa gctgttgccg
    18541 aaaccgtcgg cgaggctgtc gggcaagttc aagaagccgt tgcgacaact gaagaacaca
    18601 agctcggttg ggctgcgcgt ttgaaacaag gcttggccaa atcacgcgac aaaatggcga
    18661 aatcgctggc cggcgtgttc ggcggcggac aaatcggcga ggatttgtac gaagagctgg
    18721 aaaccgtgct gattaccggc gatatgggca tggaagccac cgaatacctg atgaaagacg
    18781 tgcgcgaccg cgtcagcctc aaagggctga aagacggcaa cgaattgcgc ggcgcgttga
    18841 aagaagcctt gtacgacctg attaagccgt tggaaaaacc gctggtcttg cccgaaacta
    18901 aagagccttt cgtgattatg cttgccggta tcaacggcgc gggcaaaacc acgtctatcg
    18961 gcaaactcgc caaatatttc caagcgcagg gcaaatccgt attgctggcg gcgggcgata
    19021 ctttccgcgc cgccgcgcgc gagcagcttc aggcttgggg cgggcgcaac aatgtaaccg
    19081 tgatttctca agccacaggc gattccgcag ccgtttgctt cgatgccgtc caagccgcga
    19141 aagcgcgcgg gatcgacatc gtgcttgccg acaccgccgg ccgcctgccc acgcagcttc
    19201 atttgatgga agaaatcaaa aaagtgaagc gcgtgctgca aaaagccatt cccggcgcgc
    19261 cgcacgaaat catcgtcgta ctcgatgcca atatcgggca aaacgccgtc aaccaagtca
    19321 aagcctttga cgacgcattg gggctgacgg ggcttatcgt taccaaactc gacggcacgg
    19381 caaaaggcgg catcctcgcc gcgcttgctt ccgaccgccc cgtccccgtc cgctacatcg
    19441 gcgtgggcga aggcatagac gacctgcgcc cgtttgacgc gcgcgcgttt gtggacgcac
    19501 tgctggattg agccgaaatg ccgtccgaaa acggca
//
LOCUS       DAXFHF010000042        18904 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXFHF010000042 DAXFHF010000000
VERSION     DAXFHF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18904
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.042"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(267..1202)
                     /gene="hemC"
                     /locus_tag="ACFIRM_001351"
     CDS             complement(267..1202)
                     /gene="hemC"
                     /locus_tag="ACFIRM_001351"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC3028967.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1321..1836
                     /gene="ybeY"
                     /locus_tag="ACFIRM_001352"
     CDS             1321..1836
                     /gene="ybeY"
                     /locus_tag="ACFIRM_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC3028968.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1838..2662
                     /locus_tag="ACFIRM_001353"
     CDS             1838..2662
                     /locus_tag="ACFIRM_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC3028969.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2805..4184)
                     /gene="nhaC"
                     /locus_tag="ACFIRM_001354"
     CDS             complement(2805..4184)
                     /gene="nhaC"
                     /locus_tag="ACFIRM_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC3028970.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4474..5697)
                     /locus_tag="ACFIRM_001355"
     CDS             complement(4474..5697)
                     /locus_tag="ACFIRM_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC3028971.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6134..6547
                     /locus_tag="ACFIRM_001356"
     CDS             6134..6547
                     /locus_tag="ACFIRM_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC3028972.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6594..7223
                     /gene="nth"
                     /locus_tag="ACFIRM_001357"
     CDS             6594..7223
                     /gene="nth"
                     /locus_tag="ACFIRM_001357"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC3028973.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7361..8860
                     /locus_tag="ACFIRM_001358"
     CDS             7361..8860
                     /locus_tag="ACFIRM_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC3028974.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9063..10583
                     /locus_tag="ACFIRM_001359"
     CDS             9063..10583
                     /locus_tag="ACFIRM_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC3028975.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRCGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            10640..11725
                     /gene="nagZ"
                     /locus_tag="ACFIRM_001360"
     CDS             10640..11725
                     /gene="nagZ"
                     /locus_tag="ACFIRM_001360"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC3028976.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLAVAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11806..11882
                     /locus_tag="ACFIRM_001361"
     tRNA            11806..11882
                     /locus_tag="ACFIRM_001361"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11840..11842,aa:Pro,seq:cgg)
     gene            complement(12218..12856)
                     /locus_tag="ACFIRM_001362"
     CDS             complement(12218..12856)
                     /locus_tag="ACFIRM_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC3028977.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CIETNGLNPVPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVGFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12853..13224)
                     /locus_tag="ACFIRM_001363"
     CDS             complement(12853..13224)
                     /locus_tag="ACFIRM_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC3028978.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QKQVQTLFGNQGLYNGFLGIGLVWSRFAVPDHAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(13490..13912)
                     /gene="queD"
                     /locus_tag="ACFIRM_001364"
     CDS             complement(13490..13912)
                     /gene="queD"
                     /locus_tag="ACFIRM_001364"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC3028979.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(14000..14515)
                     /locus_tag="ACFIRM_001365"
     CDS             complement(14000..14515)
                     /locus_tag="ACFIRM_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC3028980.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(14543..15202)
                     /gene="queC"
                     /locus_tag="ACFIRM_001366"
     CDS             complement(14543..15202)
                     /gene="queC"
                     /locus_tag="ACFIRM_001366"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC3028981.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(15387..15592)
                     /locus_tag="ACFIRM_001367"
                     /pseudo
     CDS             complement(15387..15592)
                     /locus_tag="ACFIRM_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            15691..16917
                     /locus_tag="ACFIRM_001368"
     CDS             15691..16917
                     /locus_tag="ACFIRM_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC3028982.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVPEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDTVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(16938..17306)
                     /locus_tag="ACFIRM_001369"
                     /pseudo
     CDS             complement(16938..17306)
                     /locus_tag="ACFIRM_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(17409..18904)
                     /locus_tag="ACFIRM_001370"
                     /pseudo
     CDS             complement(17409..18904)
                     /locus_tag="ACFIRM_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
BASE COUNT         4593 a         5267 c         4820 g         4224 t
ORIGIN      
        1 caataaaaaa tacgaatatc atcattctca aattcaaaat ttgaacgcca cagtattatc
       61 cgaagccaca aaaacctttc cgccgtcatt cccgcgcagg cgggaatctg gaaacccaat
      121 gctgcaagaa ttgattagaa acaactgaaa ccgagcaaac tggattcccg cctgcgcggg
      181 aatgacgaaa taaggtgagt aaaacttatt aaataaatta attaaaaaca ataacttata
      241 ttattttctt taaattttcg ataaaatcaa ttttccgtat tcaatactgc tccaatcaat
      301 tcctgcgcac cgtcgtccgc cagttttttg gcaaccgcac gtccaagcgc atcggcatat
      361 ccggcagggg cttgcgcgtc cgcctgcaac acaatcgatc cgtcgggatg cccgaccaat
      421 ccgcgcaaga tcagcagccc gttttcttca gtgcaatacg cggccagcgg cacttggcag
      481 cttccgccca aagcgcgcgc caaagcgcgt tcggcggtaa cgcaggcgtg tgtggtatcg
      541 tggtttaacg gcttcaagac ttcgtacaaa tcttcgcgat gcgtggcaat ttcaataccc
      601 aatgcgcctt gtccggcggc aggcaggctg tcggattccg acaaaatcat gcggatgcgt
      661 tcatccaatt ccagacgctg caacccggcg gcggccaaga taattgcatc gtattcgccg
      721 ttgtcgagtt tggacaaacg ggtttgtaca ttgccgcgca ggggtttaat caccaaatgc
      781 ggatagcgcg cgcgcaactg ggcttcgcgg cgcaggctgg atgtgccgac aaccgcgcct
      841 tcgggcattt cttccaaacg cgcgtatcgg ttggacacaa acgcgtcaaa cggattggcg
      901 cgttcgctga tggcggcaag ggcgaagcct tcgggcaaat ccatcggcac gtccttaatc
      961 gaatgcaccg ccaaatcggc gcgcccgtct tgaagggatt gttccaactc tttgacaaac
     1021 aagcctttcc cgccgacttt tgacaaagtt ctgtccaaaa tccggtcgcc gcgcgtggtc
     1081 atgcccaaaa tttcgacttc gcaatcggga tacagcgcct tcagacggcc ttggatatgt
     1141 ttcgcctgcc acatggcaag caggctttcg cggctggcga taacaagttt tttcgggttc
     1201 atgttcaatg cgttcaaaga atggaaacgg cgtggagtct atcatatttt gaacgtttct
     1261 tcagacggca ccgtcaggtc tatgttatat ttacgcccac aaaacacgaa acccgccaca
     1321 atgaaacgcg ccaaaaaata ccctttctta acgttgcagc ggcaacgttt ccatttgaac
     1381 tttgaaaacg cctcttccgc cgccggcatc cccgccgaac gcgattttta ccgctgggcg
     1441 tggtctgcct tgaaaaacga atacctccgc gccgacatcg gtttgattct tttggacgaa
     1501 gaagaagccc gcgcctacaa ccgcgactac cggggcaaag attatgccac caatgtattg
     1561 agtttcgcgc tcaacgaagg cgaaatcctg ccctgccaag tttcggaaag actgtacggc
     1621 gatttgatta tctgcccgca agtcgtttta aaagaagccg ccgaacaggg caaaacgcct
     1681 gaacggcatt ttgcccactt aaccatacac ggcacgctgc acctgatggg atacgaccac
     1741 atcaaagacg atgaggccga aataatggaa gccgaagaaa tccgcctgat gcgggcggca
     1801 ggctatccca acccctaccg agaggacgga cattaaaatg gacggcgcac aaccgaaaac
     1861 aaattttttt gaacgcctga ttgcccgact cgcccgcgaa cccgattccg ccgaagacgt
     1921 attaaacctg cttcggcagg cgcacgaaca ggaagttttt gatgccgaca cactgacccg
     1981 gctggaaaaa gtattggact ttgccgagct ggaagtgcgc gatgcgatga ttacgcgcag
     2041 ccgcatgaac gtattgaaag aaaacgacag catcgaacgc atcaccgcct acgtcatcga
     2101 taccgcccat tcgcgcttcc ccgtcatcgg cgaagacaaa gacgaagttt tgggcatttt
     2161 gcacgccaaa gacctgctca aatatatgtt caaccccgag cagttccacc tgaaatccgt
     2221 cttgcgccct gccgttttcg tgcccgaagg caaatctttg accgcccttt taaaagagtt
     2281 ccgcgaacag cgcaaccata tggcaatcgt catcgacgaa tacggcggca cgtcgggttt
     2341 ggtcaccttt gaagacatca tcgagcaaat cgtcggtgac atcgaagacg agtttgacga
     2401 agacgaaagc gccgacaaca tccactccgt ttccgccgaa cgctggcgca tccacgcggc
     2461 taccgaaatc gaagacatca acgccttttt cggtacggaa tacggcagcg aagaagccga
     2521 caccatcggc ggcttggtca ttcaggaatt gggacacctg cccgtgcgcg gcgaaaaagt
     2581 ccttatcggc ggtttgcagt tcaccgtcgc ccgcgccgac aaccgccgcc tgcacacgct
     2641 gatggcgacc cgcgtgaagt aagcagagcc tgcccgcacc gccgtttctg cacagtttag
     2701 gatgacggta cggtcgtttt ctgtttcaat ccgccccatc cgccaaacat aaaaatgccg
     2761 tctgaaaccg gaaaacaggt tcagacggca tttcgcttat ccgcttattt tttgctcaaa
     2821 gtcagccccg tccagccgaa taacagggtt aaagccaaac tcaaatagca gaaaaaggca
     2881 taaggcagat attcccaaac gggtacgcca agggcgtggc tgataaatac gccgcacacg
     2941 ctccacggca cgagcgggtt aatcaccgtc cccgcatctt ccagagtccg cgacaggttg
     3001 cgcgaatgca ggccgagttt gtcgtaaacg ggtttgaacg tttctcccga aagcaggatg
     3061 ctcaaatatt gctctccaat caggaaattg accccgaccg aagtcatggc aacgctgaac
     3121 gtcgcgcgtc cggcattcgt caagaaggta cggacggcct ccagcaggga aggaatcaca
     3181 ccgagcgcaa acagcagccc gcccaaactc ataccgagga taacgatggt ctgcgtaaag
     3241 aacatactct ccaagccgcc gcgcgaaatc agtttggcaa tgtctttaaa cgcttcgcct
     3301 tcgagtttgt agccgccata aaaccacgcg ccgagctgac gcagatcggg cgtgctgtgc
     3361 agatacgtta cggcaacggc ggcaatgacg gtaaagagca tggcgaccac ggcattgacg
     3421 cgcatcaatg ccaaaacgac caacagtgca aacggaatca gcgaatagcc gtgcaccaat
     3481 cccgtggctt caagctggct gcggaaggat tcgacgctgt tcaaatcctg cgcggcgacg
     3541 ctgggaagaa gccaaagcat cagtgccgcg ctgataagcc acgcggggat ggtggtgtac
     3601 atcatgtttt tgatgtgttc aaacaggtcg ataccgacga tggacgcgga aatgcccgtg
     3661 gtgtcggaaa gcggggacat tttatcgccg aaaaacacac cggaaacaat cgcgcccgcc
     3721 gtcatcgcca tatcggcctg aaacgccgcc gccatcccca taaaggcaac gccgacagtg
     3781 gcgcaggcgg tcaggctgct gccgatggac acgccgatga cggaacacag cgcgaaggcg
     3841 gaaaaataaa aataagtcgg ggaaatcagc ccgaaaccgt aatacatcag cgtcggaatc
     3901 gcgccgctca tcatcagcgc gctgaccatc agcccgatga agaaaaacag gtaaaccgcg
     3961 cccataccct gattcaacgc gcctatcatc cctgcctgca tatcgttgta tttcaaaccg
     4021 cgcgccaagc cgtacaaaat cagcacgacg atggcggcaa taatggacat atgcggcagc
     4081 cactccaatg aaatgatggt atagcccatt gcggcaatca gagcgacgac gacggcaagt
     4141 gcctcaccgc gcggcatatc gagtaaggat ttgaaagcaa acatgattct ttgcccgatt
     4201 tgatataaag ttaacggatt ctaacacacc atgccgaatc tgcccgacgt tttcagacgg
     4261 cattttagcg ttttacaaaa gccgggcaga aacaaaacgg caattttctc gatttaaatt
     4321 aatttttttg aaatataatt tttatttttt tatttgttgg catataagac ttttatggat
     4381 tgaagtatta aaacctcggc acacacgccg caaacaccgg tacgcaaaaa tgccgtctga
     4441 aagatgttca gacggcattt ctcaagaaaa agattatttg tccgccctat atccgtacac
     4501 cgcaaaaaac acgatataca ggtaacagat aacggaaacg acaaacgaag acatcagggt
     4561 gtaagtatcc gccgcccaac cttgaattac cggcacgacc gcaccgccga caatcgcggt
     4621 acacagcaca ccggaagccg cgttggtgaa tttgcccaac cctttggttg ccaaagagaa
     4681 aatcgtcggg aacataatcg agttgaaaaa accgatggca agcagcgacc acatcgccac
     4741 atccgcattg ccgctgcccg tcgccatcgc gacggcaagc agcacgaccg cagccgatgc
     4801 gttaaacgcc aaataacggt tgggcgcgaa tttcgccatt accgccgaac cgaggaaacg
     4861 tccgaccatc gcaccgcccc aataaaacga caggtaatgc gcggcagaag catgatccag
     4921 ccctttcaga taacccaaca cattgaccat cagcgaaccg atagacacct ccgcaccgac
     4981 gtagcagaaa atgcctgccg taccgaacac gagatgtttg tattgccata cgccggtttt
     5041 gccgtcgtgg ttgtgcgcgc tttcctcggc ggcaattttg cgcgcgtcgg gcagccggat
     5101 cattttcacg aaaacggcaa ggataatcag cagccccgcc agtcccaaat agggaatctg
     5161 tacggaagaa atctgttccg ccttgctgac ggtttgggtt gcgtccgcca aaatcaggaa
     5221 cgcgccgatt tgaggcgcaa tggtcgtacc caaagcgtta aacgcctgaa ccagcgtcag
     5281 cgttgccgat tccttgccgg gtttcgccag cagggtaaca taaggattac cggcgacctg
     5341 aagcagcgtt acgccggaag ccaaaataaa caacgcgccc aaaaataccg cgtaagaatg
     5401 gctgcccgca gcaggataaa acagcaggca cccgaccgcc gtcagcagaa agccgccgat
     5461 aacgccgttt ttgtagccga ctttgccgac aaaagccccc atcgggatgg acattaccgc
     5521 ataggcggta aaaaagcaaa attgaatcag catcgcctga acgtaagaga ggtcgaaaat
     5581 ttctttcaaa tgagggataa ggatgtcgtt catgcaggta ataaaaccca tcatgaagaa
     5641 cagcgtggtc aagacgacca atggggacgt atggttgttt tgtgattgcg cagacatgat
     5701 ttgcctttcg tggtgtcgga atattgcccg tttcaagaaa atagaaattg acatttctta
     5761 acggcaatgc aaatcatact gatatttgaa cattttgcaa actttatcgg atgcaggcaa
     5821 tgcgccgatc tgaaattcaa gcagcagaaa ttaatatttt acagttgttt gcataggttt
     5881 taatagattt taaaaaattc cggcgggctt gcaaaaaccg ttcccaataa aatgaaaatc
     5941 ctccgccgta tccggaaaat tcggaaatgc cgcatcgggg catcaaaacc ggagcggaaa
     6001 gaatgtcgta tgcccgacaa ataaaggggc atacggctgt gccgtatttt tgcaccgtcc
     6061 tccgcccttc ggcgggggcg tgaaaatacc gtataatccg tcgccgattt ttacctgttt
     6121 gggactgttt gcaatgatac cctcctacac ctacgccgcc ctcgcttttt ccgccttcac
     6181 ttccgccacc ctcctgcccg gcacgtccga agccgccttc gccctgttcg tccgcaactt
     6241 ccccaaacac gcatacggcg cgttgctgtg cgccggcctt gccaacggat tgggcagtat
     6301 ggtttcctat tggatgggac gtttgctgcc ttcccgaaaa atgccgtctg aaaaaacact
     6361 gaatctgata cggcgtttcg gtatttggct gcttgcgttt gcgtggctgc ccgtcgtcgg
     6421 cgacgcactg ccgcttaccg ccggctggct gcggttgaat ccgtggacaa gcgggctgat
     6481 gctggttatc ggcaaaacgg cgcgttacgc cttcattctg tggggaatgc aatattacgc
     6541 cgcctgaacc gtgcgttata atgccgaccg cacaaaccga ctgcacaaaa aaaatgaaca
     6601 gacaaatccg ccaagaaatc ttcgagcgtt tccgcgccgc caacccccat ccgaccaccg
     6661 agctgaattt caactcccct ttcgagcttt taatcgccgt tctgctttcg gcgcaggcga
     6721 ccgatgtcgg cgtaaacaag gcgacggcga agctgtttcc ggttgccgat acgccgcagg
     6781 cgatgctgga tttgggcttg gacggcgtga tggaatacac gaaaaccatc gggctgtata
     6841 aaaccaagtc caaacacatt atgcaaacct gccgcatcct gctggaaaaa tacaacggcg
     6901 aagtgccgga agaccgcgag gctttggaat cgttgcctgg cgtggggcgc aaaacggcaa
     6961 acgtggtatt gaacacagcg ttcggacacc ccgttatggc ggtcgatacg catattttcc
     7021 gcgtgtccaa ccgaaccaaa atcgcaccag gaaaagatgt acgcgaagtc gaagacaaac
     7081 tgatgcgctt cattcctaaa gaatttctga tggacgcgca ccactggctg attttgcacg
     7141 gacgctacac ctgcaaggct ttaaagccgc aatgccaaac ctgcatcatc aacgatttgt
     7201 gcgaatatcc cgccaaatcc tgaccgccgc cgcccttcaa aatgaaacgc ttgaaaattc
     7261 ggacggacgt tgtagaattt caacctatgc cgtctgaagc cgacaaaatg cgcttcagac
     7321 ggcattaaag ccggtaccgg catccgacaa aggacaaaac gtgttcaaaa aataccaata
     7381 cttcgctttg gcggcactgt gtgccgcctt gctggcaggc tgcgaaaagg caggcagctt
     7441 tttcggtgcg gacaaaaaag aagcatcctt cgtagaacgc atcgaacaca ccaaagacga
     7501 cggcagtgtc agtatgctgc tgcccgactt tgcccaactg gttcaaagcg aaggcccggc
     7561 agtcgtcaat attcaggcag cccccgcccc gcgcacccaa aacggcagcg gcaatgccga
     7621 aaccgattcc gacccgcttg ccgacagcga cccgttctac gaatttttca aacgcctcgt
     7681 cccgaacatg cccgaaatcc cccaagaaga agcagatgac ggcggattga acttcggttc
     7741 gggcttcatc atcagcaaaa acggctacat cctgaccaat acccacgtcg ttgccggtat
     7801 gggcagtatc aaagtcctgc tcaacgacaa gcgcgaatat accgccaaac tcatcggttc
     7861 ggatgtccaa tccgatgtcg cccttctgaa aatcgacgca acggaagagc tacccgtcgt
     7921 caaaatcggc aatcccaaaa atttgaaacc gggcgaatgg gtcgctgcca tcggcgcgcc
     7981 cttcggcttt gacaacagcg tgaccgccgg catcgtgtcc gccaaaggca gaagcctgcc
     8041 caacgaaagc tacacaccct tcatccaaac cgacgttgcc atcaatccgg gcaattccgg
     8101 cggcccgctg ttcaacttaa aaggacaggt cgtcggcatc aattcgcaaa tatacagccg
     8161 cagcggcgga ttcatgggca tctcctttgc catcccgatt gacgttgcca tgaatgtcgc
     8221 cgaacagctg aaaaacaccg gcaaagtcca acgcggacaa ctgggcgtga ttattcagga
     8281 agtatcctac ggtttggcac agtcgttcgg tctggataaa gccagcggcg cattgattgc
     8341 caaaatcctt cccggcagcc ccgcagaacg tgccggcctg caggcgggcg acatcgtcct
     8401 cagcctcgac ggcggagaaa tacgttcttc cggcgacctt cccgtcatgg tcggcgccat
     8461 tacgccggga aaagaagtca gcctcggcgt atggcgcaaa ggcgaagaaa tcacaatcaa
     8521 agccaagctg ggcaacgccg ccgagcatac cggcgcatca tccaaaacag atgaagcccc
     8581 ctacaccgaa cagcaatccg gtacgttctc ggtcgaatcc gcaggcatta cccttcagac
     8641 acataccgac agcagcggca aacacctcgt cgtcgtacgg gtttccgacg cggcagaacg
     8701 cgcaggctta aggcgcggcg acgaaatcct cgccgtcggg caagtccccg tcaatgacga
     8761 agccggtttc cgcaaagcta tggacaaggc aggcaaaaac gtccccctgc tggtcatgcg
     8821 ccgtggcaac acgctgttca tcgcattaaa cctgcaataa cgcacataat ttaaccaaaa
     8881 tatttttaaa tgataaaatg cccgtcaata ccgattaacg ggcattttac aaaaatcagt
     8941 tggttgattt taaacaaata ttttttacga caaaaaatat ttccacccat tttgccaaca
     9001 aaacccaaac cggaggcgga cagataccca cccatccgga cgcttaacac cccccataaa
     9061 ccatgcagct gattgactat tcacattcat ttttctcggt tgtgccaccc tttttggcac
     9121 tggcacttgc cgtcattacc cgccgcgtac tgctgtcttt aggcatcggt attttggtcg
     9181 gcgttgcctt tttggtcggc ggcaaccccg tcgacggtct gacacacctg aaagacatgg
     9241 tcgtcggctt ggcttgggca gacggcgatt ggtcgctggg caaaccaaaa atcttggttt
     9301 tcctgatact tttgggcatt ttcacttcac tgctgaccta ctccggcagc aatcaggcgt
     9361 ttgccgactg ggcaaaacgg cacattaaaa accggtgcgg cgcgaaaatg ctgaccgcct
     9421 gcctcgtgtt cgtaaccttt atcgacgact atttccacag cctcgccgtc ggtgcgattg
     9481 cccgccccgt taccgacaag tttaaagttt cccgcgccaa actcgcctac atcctcgact
     9541 ccactgcctc gcccatgtgc gtgctgatgc ccgtttcaag ctggggcgcg tcgattatcg
     9601 ccacgcttgc cggattgctc gttacctaca aaattaccga atacacgccg atggggacgt
     9661 ttgtcgccat gagcctgatg aactattacg cgctgtttgc cctgattatg gtattcgtcg
     9721 tcgcatggtt ctccttcgac atcggctcga tggcgcgttt cgaacaggct gcgttgaacg
     9781 aagcccacga cgaaaccgcc gcttcagacg ctaccaaagg tcgtgtttac gcattgatta
     9841 ttcccgtttt ggccttaatc gcctcaacgg tttccgccat gatctacacc ggcgcgcagg
     9901 caagcgaaac cttcagcatt ttgggggcat ttgaaaatac cgacgtaaac acttcgctgg
     9961 tattcggcgg cacttgcggc gtgcttgccg tcgtcctctg cacgttcggc acgattaaaa
    10021 ccgccgatta tcccaaagcc gtgtggcagg gtgcgaaatc catgttcggc gcaatcgcca
    10081 ttttaatcct cgcctggctc atcagtacgg ttgtcggcga aatgcacacg ggcgactacc
    10141 tctccacgct ggttgcgggc aacatccatc ccggcttcct gcccgtcatc ctcttcctgc
    10201 tcgccagcgt gatggcgttt gccacaggca caagctgggg gacgttcggc attatgctgc
    10261 cgattgccgc cgccatggcg gtcaaagtcg aacccgcgct gattatcccg tgtatgtccg
    10321 cagtaatggc gggggcggta tgcggcgacc actgttcgcc catctccgac acgaccatcc
    10381 tgtcgtccac cggcgcgcgc tgcaaccaca tcgaccacgt tacctcgcaa ctgccttatg
    10441 ccctgacggt tgccgccgcc gccgcatcgg gctacctcgc attgggtctg acaaaatccg
    10501 cgctgttggg ctttggcacg accggtattg tattggcggt gctgattttt ctgttgaaag
    10561 ataaaaaacg cgccgacgtt tgaccttccc ccctgttcag acggcatatg ccgtctgaag
    10621 ttttttggag caaaccgcaa tgaccgtccc ccatattccg cgcggccccg tcatggcaga
    10681 cattgccgcc ttccgcctga ccgaagaaga aaaacaacgc ctgctcgatc ccgcaatcgg
    10741 cggcatcatc ctcttccgcc gcaacttcca aaacatcgaa caactcaaaa cactcaccgc
    10801 cgaaatcaaa gccctgcgca cacccgaact cattatcgcc gtcgatcacg aaggcggcag
    10861 ggtgcaacgg tttatcgaag gcttcacccg cctgcctgca atgaacgttt tagggcaaat
    10921 ttgggacaaa gacggcgcgt ccgccgccga aaccgctgcc ggacaagtcg gccgggtttt
    10981 ggcaaccgag ctttccgcct gcggcatcga tttgtccttc acgcccgtct tggatttgga
    11041 ctggggaaac tgcgccgtca tcggcaaccg cagcttccac cgcaatcccg aagcggtcgc
    11101 ccgcctcgcc gtcgcccttc aaaaaggttt ggcaaaaggc ggtatgaaat catgcggcaa
    11161 acatttcccc ggacacggat ttgtcgaagg ggacagccat ttggtgctac ccgaagacgg
    11221 gcgcagcctc gacgaactcg aagccgccga cctcgccccc ttccgcatta tgagccgcga
    11281 aggtatggcg gcggtcatgc ccgcccacgt cgtttatccg caagtggaca caaagcccgc
    11341 agggttttcc gaaatctggc tgaaacagat tttgcgccgc gacatcgggt tcaaaggcgt
    11401 gattttctcg gacgatttga ctatggaggg cgcgtgcgga gtcggcggca tcaaagaacg
    11461 cgcccgcatt tcctttgagg caggttgcga catcatattg gtctgcaacc gtcccgactt
    11521 ggtcgatgaa ttgcgcgacg gtttcaccat tcccgacaac caggatttgg ccggacgttg
    11581 gcaatacatg gaaaactcgc tcggtcatga ggcggtacaa gccgttatac agacaacggg
    11641 tttccaagcg gcgcaagcct ttgttgccgg ccttgcctcg ccgcaggaca cggcgggcgg
    11701 cgtaaaagtc ggagaggcct tttaaatccg cccgacacgc ggcaaaaagg caaaacggta
    11761 taggcacgcc gtattttttg cgctataatc acaccttaat taattcggaa tgtagcgcag
    11821 cccggtagcg cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tcattccgac
    11881 catattcaga ccgcctcgac atcgattgcc ggggcggttt ttcccttttg gcggcaacaa
    11941 aaaaatgccg tctgaaacag aatgctgttt cagacggcat ttcgttttcc ccaaccctgc
    12001 tatttgcgta cactgacaaa gggtatcggc aaggttcaaa agaccatcgg aatctaggaa
    12061 gcctggcgtg tacccaaccg cggcatctta ttcattaagg cttcgtcctg attgggcggc
    12121 ggaaatgtcg gcagtttcac gcggaagcgg cacactttcc ccgtatgtca aacatcggca
    12181 aaacggtacg ccggcccttt gagttacact gcttaaacta ctctattccc gcccatttgt
    12241 gcgtctgcac gctcaactgc caatgcacgg gcgcgtcggg gcggctgttc agaataccga
    12301 tttggcggat ggtgtcgtaa atgttcattg cgccgtcttg ttcgcagggc gaaaggtagt
    12361 aatgatgcgc gcggatttta cgttccatat tttcgcagaa tccgacgaca tcgccgtcgg
    12421 caacaatccg cacttcgtcg gctgtttcga tacagctttt ttcgtatttg gcggcataac
    12481 aggctttggg gctggtggcg acatagtcga tttgcggcgg cacgggattg agtccgttgg
    12541 tttcgataca gaggaaatag ccttccgcct tgagcgtgtc cagcagcata tcgagatgcg
    12601 gctgtatggt cggttcgccg ccggtgatga tgatgttgcg ggcggcataa gttttcagac
    12661 agcctaagat gtcggacagg ctcatcatac caaatgtcaa ataatcggta tcacaccagc
    12721 cgcacgccag attgcatttg cccaagcgga cgaaaacggc aggcatgccc gtgttccagc
    12781 cctcgccttg caggctttca aagatttcga cgatgcggta ttgcggattt tcgggggcga
    12841 cgctgatttt tttcataccg ccaacaccgc cgccgctgcc aaaaatgcgg gcaaaccctg
    12901 tttgacaagt atgcctttgt tgccggacga aaacgcaccc cacgcggcgg caatcaggac
    12961 gaagccgaga aacagtaccg tcgcgccgta cacggcatga tccggcacgg caaaccgcga
    13021 ccacaccagc ccgatgccga gaaagccgtt atacagccct tggttgccga acagggtctg
    13081 cacttgcttt tgttccataa attcataagg cagcttgaac gtttccgccg ctttttcgct
    13141 gggaatctgc gtcatttcca gccaggcgat gtagaaatgt tcggcggcga cgaggaggac
    13201 gagaatggtg gagaggagtt tcatgggtgt tccttttaat gggtgtgccg gtatgaaatg
    13261 ccgtctgaag aacggagaat ccgcacagga gaaaaaccgc tttgcggtaa attcaaataa
    13321 ggacgctcca cactgtcatt cccgcgcagg cgggaatcca gagatttgga attgctgcaa
    13381 tttttaaata ttcctgataa atcaaggtct ggattcccgc ctgcgcggga atgacgcggt
    13441 tgggcatgtc ttatttgaat ttactatatc gatacgttca agattttccc tactcccctt
    13501 catactccgc gcacgatgtc ggcgtttccc acaatttgac gcggcacacg ttcagccccg
    13561 cgtttttcag acggcagtac atttcgaccg ccatattttc ggcagttgtg cggcagggca
    13621 ggcgcagggt tttcatattc cagccttcca aaagcgcggc gatttgagat tcgcgcccgt
    13681 tgccgccgtg gtagatgaag gcgtggtcga aatggtcggt aatgtgttgt ttcacaatgg
    13741 ctttcaagtc ggtaaagtcc atcaccatac cgtctttcgc tccgcctttg atggtgccgt
    13801 ctgaaacggt gatttcgagt ttgtaggtat gcccgtgcag gttttggcat ttgccgtcat
    13861 gcccgtcgag catatgcgag gagtcgaagg taaatatctt ggtaattttc atcccgattc
    13921 cttttcagtg aaaaccccgc cgctttccgc ccggagggtg gcttgatgtg tcgttttgcg
    13981 tggaaacata tccgctgctt caaatgggca aatagccttg aaagccgatt tcggcgggtt
    14041 tgccgtgcgg attcggggtc aggcggatat ggaaattgcc gatgtgctcg agcgcaagca
    14101 ggttgtccac gtctttcaag ttgtctttat gctgccgaac atagtcggtc aacagggttt
    14161 gcagggcttt ttgtttggct tcggcaggcg tggcggcggc gaacagcccg aacgcgtgcg
    14221 cctcggcaag ctgccccgcg cgatagccgc cgacattggc aaaatacagg cgcggagcgt
    14281 tttcaggcgg cattttcgtt ttgggcgttt cggatacggc gatgtcgtaa ccgtccgccc
    14341 attcgacaac ctgccaaccg tcgatgtgga ttttgtccgt atcgccgaac cacgcggctt
    14401 tgagtgcggg gacggcttcg cggtagtcgt cgcacacggc aaattggatg tcgtgcactt
    14461 cgatattcga ccggccggca ttgccgccga ggtaaaacat atggagcttg ggcatatcgt
    14521 attccttgtg ctttgcgcgc gttcagacgg cctttttact ttccagacat tccgccaacc
    14581 cgcgttcgcg caagatacag ctcgggcatt cgcggcagcc gccgacgata cccttgtagc
    14641 aggtgtgggt ctgttcgcgg atgtagtcca acacgcccat ttcgtccgcc aacgcccacg
    14701 tttgcgcctt ggtcaggtac atcagcggcg tgtggatttg aaaatcgtag tccatcgcca
    14761 gattaagggt aacgttcatg gatttgacga acacgccgcg gcagtcggga tagcccgaaa
    14821 aatcggtttc gcacacgccc gcgatgatgt gccggatacc ctgccctttg gcaaaaatgg
    14881 cggcgtaaag caggaaaagc gcgttacgcc cgtccacaaa ggtattggga acgccgttgt
    14941 cggcggtttc gatggcggcg gtttcgtcca tcagggcgtt gtgcgtaatc tgccgcatca
    15001 ggctcaaatc gagtacggtt tgactgacac ccaaatcctg cgcgatccac tctgcgcgtt
    15061 ccagctcgac ggcatggcgt tgcccgtatc ggaaggtgat ggcttggacg ttttcgcgcc
    15121 cgtaggtttg gattgcctga atcaggcagg tggtcgaatc ctgaccgccc gagaagatga
    15181 ccaaggcttt ttggtttgac atatcgaatc ctagttttgg taacgcggga ggtttgcgaa
    15241 ccgcgaagtt ggacagtaaa gacgcggatt atacaggcaa acgcgttcag acggcatctg
    15301 aacgccatgc cgtctgaaca cgcggcgtat gccgacttga cggcgagttc cgccaagcgt
    15361 ctgccttgtg cgaaggcgat attgttttct tcggcggtca ggacgggagc cgctgtcggg
    15421 aatatcttta tttgacggct tcgcagacgg cggacacttt ggggacggtg cgcaatacgg
    15481 cttcgcaacc ttcaacgctg tcgatgccgc gagtgattcg gcgtgcggga ttgcgggtgc
    15541 tgccgttttg ggaatagtag aggacgagga ttttgagggg atttgggttc atgggcggat
    15601 tccgttaaaa tagtttggtg ttcagacggc ataacggttg tccagatgcc gtctgaaaca
    15661 gtttgaaaac atgaaacctg gtaagagatt atgacctttt tacaacgttg gcaaggtttg
    15721 gcggacaata aaatctgtgc atttgcatgg ttcgtcatcc gccgtttcag tgaagagcgc
    15781 gtaccgcagg cagcggcgag catgacgttt acgacactgc tggcactcgt ccccgtactg
    15841 accgtaatgg tcgcggtcgc ttcgattttc cccgtgttcg accgctggtc ggattcgttc
    15901 gtctccttcg tcaaccaaac cattgtgccg cagggcgcgg atatggtgtt cgactatatc
    15961 gacgcattcc gcgatcaggc aaaccggctg accgccatcg gcagcgtgat gctggtcgta
    16021 acctcgctga tgctgattcg gacgatagac aatgcgttca accgcatctg gcgggttaac
    16081 acgcaacgcc cctggatgat gcagttcctc gtttattggg cgttgctgac tttcgggcct
    16141 ttgtctttgg gtgtgggcat ttcctttatg gtcgggtcgg ttcaagactc cgtactctcc
    16201 tccggagcgc aacaatgggc ggacgcgttg aagacggcgg caaggctggc tttcatgacg
    16261 cttttgctgt gggggctgta ccgcttcgta cccaaccgct tcgtgcccgc ccggcaggcg
    16321 tttgtcgggg ctttggcaac agcgttttgt ctggaaaccg cgcgttccct cttcacctgg
    16381 tatatgggta acttcgacgg ctaccgctcg atttacggcg cgtttgccgc cgtgccgttt
    16441 ttcctgttgt ggttaaacct gctgtggacg ctggtcttgg gcggggcggt gctgacttcg
    16501 tcgctgtcct actggcaggg cgaagcgttc cgcaggggct tcgactcgcg cggacggttt
    16561 gacgacgtgt tgaaaatcct gctgcttctg gatgcggcgc aaaaagaagg caaagccctg
    16621 cccgtacccg agttcagacg gcatatcaac atgggctacg acgagttggg cgagcttttg
    16681 gaaaagctgg cgcggcacgg ctacatctat tccggcagac agggttgggt gttgaaaacg
    16741 ggggcggatt cgattgagtt gagcgaactc ttcaagctct tcgtgtaccg cccgttgcct
    16801 gtggaaaggg atcatgtgaa ccaagctgtc gatacggtaa tgacgccgtg tttgcagact
    16861 ttgaacatga cgctggcgga gtttgacgct caggcgaaaa aacagcagca gtcttgaata
    16921 tttttgaaac tgtattttta tcctaaagac attttcctat ctgctgattc caccgtttcc
    16981 tttctgtgtg ccgccataat ttttatgata gacaagccat tcaagtttgc attgactgct
    17041 ttttcattgg caatatccaa atgactggcc gcttcatcta aaaataggat tttcggttcg
    17101 caatataagg cccgcgccaa tacgatacgt tgtttttgtc cgcctgacag tgcgctgccc
    17161 atatcgccga tcaaggtttc atagcccata ggcatggcgg agatttcttc gcgaatcata
    17221 gcaaggcagg cgcaatgttc gatttttcct ctatccggtt ctgtatcgaa aaaacagata
    17281 ttctctgcaa ttgggccagc aaatatagtg gattaaattt aaatcaggac aaggcggcga
    17341 agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
    17401 atccactata aaacatcgtc ctgcctgacc atcgcactca aattgcggat aaaacgtggc
    17461 ggtaagctgt aaatgtcgtg cccatttatc atgactttgc ctgattcggg aggtagattg
    17521 ccgcttaaaa tatccaaaag tgtcgattta ccgcagcccg atcgtccgat taacgcaagc
    17581 gattcaccac gtccgatctt gaagctgacg ttttgtaaaa catatggctc gttatcggca
    17641 taacggaaag aaacattttc cactgagatt tcgatgtcgt ctgaaatatc aggaatgcct
    17701 acatccggcg tagcttccga ttctgtttca ttcaatacgg tgtcagctaa ccgttccgca
    17761 tgaagtgaca gcattttaat ttgcacaaac tgatcgacca gcgagccaat acgtccttca
    17821 aactgtccct tataagccaa aaaagccatt agtacgccga cggtaaagac gccgtccaat
    17881 accatgcccg cagctaagta aataatcaaa atatttgcca aaccgaaaag cagcttgttt
    17941 gccaagccga accaaattcc cagtttctgc accgtcagtc ccgtattaac cgtatccacc
    18001 agcagcccca tccatgcaga atatcgttga ggacctttac caaattgctt gactgctcgg
    18061 ataccgcgta ccgtttccat aaaatagctg ctttgtttgg cagcatggat gatgttttcc
    18121 tccgtcgccc gtcgcaacgg ataataggct acccaacgga ctgcgatata aactaccagt
    18181 gtcgctgcaa ccaaacctgt cagtatcggg ctgtacacta tcattagcac aaaagtaaaa
    18241 acagccgtta ggctgtttaa aaccaacacg aaaaacgttg aagtcagagt tccctgaata
    18301 ctatccaccg ccccgaagcg cgataccaca tcgcccaaat gccgtttagc gaaataatct
    18361 gtaggcaaat ctatcaaacg tttgaaaacg tttgatttcc actgaacatt taacgtcgtt
    18421 gaaaaatgca tgcccaacca gccctgcatg acagacaccg tattttgtac cagaagtagc
    18481 aaggtaaagc ccaaagccag cgtcgctaac agattacgat cgcccgttac cactacatgg
    18541 tcgatgaccc attgcataaa aaacggcgac accaaagcaa acacttccat tgctgctgcc
    18601 aaagccaaaa gctgaaacaa tgtccgcccc agcccagaaa tcccgcgcaa catgggtagg
    18661 atgcggattt cctgcttttc ttcccctgcc tcgaaacgcg tgtttggcca cagttccaaa
    18721 gcaattcccg taaacttgcg cgatatttcc tccgtcttga ctttgcgtcg tcccgaagcc
    18781 ggatccatga cggcagcccc gtccgaagat accgattcca gcaccacaaa atgattcaaa
    18841 ttccaatgta gaatacaggg caggcgcaaa ctgcccaatt cgtctaaatc cagcctcaac
    18901 gccc
//
LOCUS       DAXFHF010000043        18832 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXFHF010000043 DAXFHF010000000
VERSION     DAXFHF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18832
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.043"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(225..1301)
                     /locus_tag="ACFIRM_001371"
     CDS             complement(225..1301)
                     /locus_tag="ACFIRM_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC3028983.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            1365..1502
                     /locus_tag="ACFIRM_001372"
     CDS             1365..1502
                     /locus_tag="ACFIRM_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC3028984.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            1524..2666
                     /gene="bioF"
                     /locus_tag="ACFIRM_001373"
     CDS             1524..2666
                     /gene="bioF"
                     /locus_tag="ACFIRM_001373"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC3028985.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            2676..3323
                     /locus_tag="ACFIRM_001374"
     CDS             2676..3323
                     /locus_tag="ACFIRM_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC3028986.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            3311..4093
                     /gene="bioC"
                     /locus_tag="ACFIRM_001375"
     CDS             3311..4093
                     /gene="bioC"
                     /locus_tag="ACFIRM_001375"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC3028987.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            4314..5751
                     /locus_tag="ACFIRM_001376"
                     /pseudo
     CDS             4314..5751
                     /locus_tag="ACFIRM_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            5754..6852
                     /locus_tag="ACFIRM_001377"
                     /pseudo
     CDS             5754..6852
                     /locus_tag="ACFIRM_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            6864..7399
                     /locus_tag="ACFIRM_001378"
                     /pseudo
     CDS             6864..7399
                     /locus_tag="ACFIRM_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            7401..8043
                     /locus_tag="ACFIRM_001379"
                     /pseudo
     CDS             7401..8043
                     /locus_tag="ACFIRM_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            8064..8423
                     /locus_tag="ACFIRM_001380"
     CDS             8064..8423
                     /locus_tag="ACFIRM_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC3028988.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            8515..8590
                     /locus_tag="ACFIRM_001381"
     tRNA            8515..8590
                     /locus_tag="ACFIRM_001381"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8548..8550,aa:Asn,seq:gtt)
     gene            complement(9088..9999)
                     /gene="crgA"
                     /locus_tag="ACFIRM_001382"
     CDS             complement(9088..9999)
                     /gene="crgA"
                     /locus_tag="ACFIRM_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC3028989.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            10361..10816
                     /locus_tag="ACFIRM_001383"
     CDS             10361..10816
                     /locus_tag="ACFIRM_001383"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3028990.1"
                     /translation="MIDAGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVF
                     TGGHGKLYQSDGRHRVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGV
                     DVLYMHFHKANEFIGLSRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(11024..12409)
                     /gene="pntB"
                     /locus_tag="ACFIRM_001384"
     CDS             complement(11024..12409)
                     /gene="pntB"
                     /locus_tag="ACFIRM_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC3028991.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLEGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(12489..12872)
                     /locus_tag="ACFIRM_001385"
     CDS             complement(12489..12872)
                     /locus_tag="ACFIRM_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC3028992.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(12876..14414)
                     /locus_tag="ACFIRM_001386"
     CDS             complement(12876..14414)
                     /locus_tag="ACFIRM_001386"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC3028993.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            14703..15536
                     /gene="serB"
                     /locus_tag="ACFIRM_001387"
     CDS             14703..15536
                     /gene="serB"
                     /locus_tag="ACFIRM_001387"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC3028994.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFGQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            15600..16822
                     /locus_tag="ACFIRM_001388"
                     /pseudo
     CDS             15600..16822
                     /locus_tag="ACFIRM_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            16983..18563
                     /gene="purH"
                     /locus_tag="ACFIRM_001389"
     CDS             16983..18563
                     /gene="purH"
                     /locus_tag="ACFIRM_001389"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC3028995.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHVNPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TCIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         4646 a         4505 c         4983 g         4698 t
ORIGIN      
        1 tctgggatat tggtaaaggg aaaaataaaa taaatttcca atctgtgttt atttgattgg
       61 gcgactatta tcgtgaaata tgccgtctaa agccttcaga cggcatattt gtgcgcttgg
      121 atgttgcaga aagcggcagg gcggcggtgt aggaaaagcc aaacaaaaac caaaccgcct
      181 gtcaacttct gataaacata agtattaaat aatcagaagg ttattcaatt acctaaacgc
      241 aaattcccct gccgtatcac atctattgaa aataatacat caaccggctc ggaagcagcc
      301 tgatcaggtg attctacttg cggcgatgaa tcggcagccg gttcggtata ggcagtcggc
      361 gtgccgtcgg attgggtatt taaatgcgct tgtgcctcgg gcgtgtgtac atcaggcact
      421 tcgggcgtgc gtgtctcgga tacttcggca gagttggttt cctcagtttg ttcaatgact
      481 tcagcttggc tgtatgagga agaaccctgt atccacgcca gcgatttgag cggcatcttc
      541 atcttgccgt ttttgccgca ggtcagacag acggccgatc cggtgcggtc tttgagtacg
      601 gcatcgactt tctcgggcgc gatggttttg ccctgtgttt ttgcccattg cccgatgctt
      661 tttttcaggg aggcggtgtc aaggttgttc ttaccgtaag ggtcgcggaa ggcggcaagc
      721 cacaggttgc gatccgcatc ttcattgagg cccgccattt gatagatgac ggaggcgggc
      781 gagccggtgg cgatggtttt ggcaaactcg agcgcgtcgg gcgatttcat gcggacgcag
      841 ccgtgactcc ggatgcccgg gacgctggcc ggtgcattgg ttccgtgtat gcccaaaccg
      901 agtttcgggt ctcctaagcg gacaaaaacc ggccccaaag ggttgtccgg gccggcggct
      961 atggttttta cgccgtcgcc gcgttctttc tgtatggatt ttgggatgtg ccaaacaggg
     1021 ttataggctt tcgccccgat tttatgttcg cctagattgg tttgtgtcat cgcccgacct
     1081 actgcaacgg gataaacctt ggtcagtttg ccgtcggtgt agaggaacag gcgttgctga
     1141 gggatgttaa tgaagacatg ttgaccttgt gcgacggggg agacatcggg aatgacggtg
     1201 tttgcgtatg aaaaaccgct tatcaatagt gcggcagtgc ggcagattgt tttattcata
     1261 tcaaaatatg gtgtgtgtcc gataggtttt cggcaaatca tacctgaaac cgtaccaatt
     1321 tgtgcgaaaa tatgcgcttc ggtacagtgc ggacggattg ggtaatggca acggaaacaa
     1381 atatcgcgga aatttccgcc ttggattatg aaggcagggg tgtggcaaag gtcggcggca
     1441 aaacgatttt cattaaaggg gcattacttg attgtttgat gctgggttgc ttcaggcttt
     1501 aactcaggaa tatttacatc ataatgaaag tttttagaca acagcttgaa caactcggcg
     1561 cgcaaaatca atatcgttcg attccggatt tgattcatca agggcggtat attacgcggg
     1621 aaaaccgcaa aatgctgaat atgtcgtcta atgattattt ggggttggca tcagatgaaa
     1681 acttacgccg gtcttttttg cagcaatacg gcggtaattt tccctctttt accagttctt
     1741 catcacgttt attaacgggc aactttccta tttataccga tttggaagag cttgtcgcac
     1801 aacgtttcca acgggaaagc gcgttattgt tcaacagcgg ctatcacgcc aatctcggta
     1861 ttttgcctgc tttgacgacg acgaaaagtt tgattttggc agataaattc gttcacgcca
     1921 gtatgattga cggcatccgt ttgagccggt gtgcgttttt ccgttatcgt cataatgatt
     1981 atgaacattt gaaaaatctg cttgaaaaaa acgtcggaaa atttgaccgc acttttatcg
     2041 ttaccgaatc tgttttcagt atggacggcg atgtggcgga tttgaaacag cttgtccaat
     2101 taaaaaaaca gtttcccaat acttatcttt atgtggatga agcccacgca atcggtgttt
     2161 atgggcaaaa cggattgggg attgccgaac gggataatct gattgccgag attgatttat
     2221 tggtcggcac tttcggtaaa gccttagcct cggtgggggc gtatgccgtc tgcaaccaag
     2281 tattgaaaga atgtttgatt aatcaaatgc gcccattgat tttttcaacc gcattgccgc
     2341 cgtttaatgt ggcttggact tattttattt ttgaacgatt gccgcaattc tcaaaagaaa
     2401 gaagtcatct tgagcagtta agcgcatttt tacggcggga agtggcgcat cggacgcaaa
     2461 taatgccgag cgaaacctgt atcgtcccct atattttagg cgggaatgaa gccacccttg
     2521 ccaaagcgga atatctgcaa ggtcagggtt attattgcct gcccatcaga ccgccgacag
     2581 taccaaaaaa cacatccaga atccgcctgt ctttaactgc cgatatgaca acggatgaag
     2641 tgcggcagtt tgcggcgtgc ctgtaaggat atgatatgaa aacaaaattt tacaatcatc
     2701 aaggcgaaca tttaatcctg tattttgcag gttggggaat gccgcccgat gctgtaaatc
     2761 atttgatttt gccggaaaat cacgatttat tgatttgcta tgattatcaa gatttaaatt
     2821 tggattttga tttttccgcc tatcggcaca tccgtttggt ggcgtggtca atgggcgttt
     2881 gggcggcaga gagggcattg caaggaataa gattaaaatc cgcaacggca gtgaatggca
     2941 caggtttgcc ttgcgatgat aatttcggta tcccttgcgc cgtttttaaa ggcacattgg
     3001 agaatctcac agaaaatact cgttcaaagt ttgaacgtag aatctgtggc gataaagcat
     3061 cttttgaaga ttaccaacaa tttcccgccc gtccgtttga cgaaattcat caagaactta
     3121 ccgcactttt tgcgatgatc gggcaagata ggcgtacaga tcttatccgc tggacaaacg
     3181 ccttgttcgg atcgggcgat aaaattttta ttcctgccaa tcagcaccga tattggacac
     3241 cgcgttgcac cgttcaagaa acagacggcg ggcattacct gttttcaaga tttacccatt
     3301 ggtcggcact atggaatcac tgactgccat aaataaaccg cgcattcggc aggctttcca
     3361 aaaagcatta agcgattatg acaggcacgc cttaatccaa caaaaaatga cgattaatct
     3421 aatcgcgcat ttgcaagatt atttgccgga tatgccattg gaaaacgtgt tggaattggg
     3481 ctgcggctca ggaatgttga gtgccttgct gcaaaaacag atttcagcga attattggtt
     3541 atttaatgat ttgtgcgatg tacgaagccg actggctgaa aaactgcctc aatcctttga
     3601 tttttattgc ggcgatgcgg aaaattttcc ttttcaacga caatttgact taatcgcaag
     3661 cgcatctgcc gtgcaatggt ttcaccaacc cgacgctttt atcgcccatt gcaaaacagg
     3721 cttgaaaaca aacggattat tggcggttgc gacctttggc aaagacaatt taaaagaagt
     3781 ccgccaaatc acaaatatag gcttaaatta cccgacttta tcccaatggc aggcttggtt
     3841 agccaaagat tttgagcttt tatggtgtga ggattttaag gtaatactag actttgatac
     3901 gccgttagat gtactcaaac accttaaata tacaggcgtt accgccacga accaaaaaaa
     3961 ttggacaaga aaaaatctca atggatttat tggcgattac ttgtcggcgt tcggtatgcc
     4021 gtcgggcaaa gtgcgcctga cttatcatcc gttatttttt atcgcgcgcc actctgcggc
     4081 gggcaggcaa taaggcagct tatgggcaaa gttattttta tatcgggtat tgatactgat
     4141 gtgggtaaaa ggtaatatgg cgagccttat gcagaaggca tattgtcaaa cgttaaatta
     4201 tggtatgatt taaaatttac aagtctattt cagtaaatcg ttaataataa aagcggacaa
     4261 ttggccgttg caggcggtca gctatccgct cgttacggta tcgggggttt cctttggaaa
     4321 actcattaat tatcggtctg gcactggcgg tattgctgat gcttttggtt atgcgtgcga
     4381 agcagggtaa gaaaacaccg aagcgcaaat cccaaggtgt cggcaatacg caggggcagc
     4441 cgcccggaag caatgattcg gactgggtcg atcagacttg gtcaatcggt ttcagacggc
     4501 acgcaactcg actggtcttg gaacgaaagt gccgagaccg catccgccgc cgtatccgcg
     4561 caagaagtcg atccgcttac ggagtatcag gtttataagc aattcggtta tcagggcaag
     4621 gctgccgaat ctttggctgc ctatctggac ggcattccgg atggtgaagc gaaacctgag
     4681 aaccttatcc gcgagctgat cgatatcaat ctcgaagtgg gggatgtcga tgttttggca
     4741 gacaatctgc aaaaatacgg cagactgatt cctttcgaac ttttggcaaa atatatcgaa
     4801 caggcattac agcgagattc aaaccatttg cgtatccgcg tcttggcgga agaaggtttg
     4861 ggatagggca ctcaggagat tgaaaaacgt gcggaaggcg gttctgcgac ggcagcttcc
     4921 gcatcgcccc cgccggatgt cggcggtaag gcttatggag ccgaagaaat caagcgcatc
     4981 ccgattgtgc ggggcaaaaa agacgtgtcc ggaatcagcc aagagaaaat cggtgcgatt
     5041 gccggcctgg tccgcgccga tcaaggtgcg aaaatcctta aagacaaagt cagctacgaa
     5101 acggcatcga aacaatacga ccgtgccatc caaacttccg aaaaacctgc aaacctgatt
     5161 atcgatgcgt tgaaactcga ttaccaacac gcggacatag accgttttgc cggacatttg
     5221 tggaaacttt accaaacgtt gggcaactac ggcaggcagg ttaaagagcg gatgctgggt
     5281 taggggtaca gcctgggtta ccatgaagtt ttcgatgatt tggaaaaggg gccgaacgat
     5341 cggcaaatca aagacatcgg tatgaggcac gggtatctgc cgaaaaatat acagaaattc
     5401 aaagcgcaac atcgggattt ggtgcttcaa gattcttcgt tgatcaacac tggttcgtct
     5461 ccggcagacg atgcggttaa agaagtagag tcgttgctga tgtatggtca gattgaagcg
     5521 gcaatggatg tgttggagca ggcggtattg aaatatcccg acgaatccca gctttatatt
     5581 acgttgatcg atatttatga acgtactgaa gattgggata ggttggggca gtttttaagg
     5641 gtattgaggg aacgtgcgga caggcttcct gaagaggtcg ttatgctgat gagccggctg
     5701 ctgcagcgta tgaatcaaaa tattaaaaaa ataaaacggt acggaaaata aaaatggaag
     5761 ttcaactgcc gaaaattaaa acagtacgcg taatgttggc ggggatgacg gcgcagcagg
     5821 aatccgtttt caaaatggca ttcaaaatgc acaataccac ccgttatgaa acagtatccc
     5881 cttcagacgg cagtgccgtg cccgatttgg ttttggcgga taccgatgcc gagggcggtt
     5941 ttgaactttg gaaagagctt gccgggcgtt ataaggctat acccgtcgcc gtctgttcgg
     6001 agaaagttcc cgattctgaa gttccctacc tgcccaaacc gattcggttt gaaacattgt
     6061 ttcctatgct ccgcaagtta ttgcagggcg agaatgttta tgggaaatcg tttattgcac
     6121 ccgcagaccg gtcggcgaaa aacaacggga atgtgcagcg tacggttacg atacgccagt
     6181 ttaacccgaa taaaggatta ttgggggcgt tgcggtttgc ggaaaagaac aggcaggaca
     6241 tcgctatctt gcatggaaat aagccggtcc ttaattgttt tcccctcgat acaacgggtt
     6301 ttgctgacag aaagtgtgta aaaactcgaa gaattgtgca aagacgaaaa tttgcaggtc
     6361 agttgcaaga ctgttcccga taactcgcaa tggcgcgaaa aggctaaagt aggcattatg
     6421 tcctgtatgt ggcagttttc catttggacg gcgcaggaca ggttgattta tccgattttt
     6481 cccgatactc cgtttacgtt gaaatcttgg ccaaacctga cccggtaggc aaatgtgccg
     6541 gggtcgatac gcttgtcggc atttttgacc aaggcatccg tcaaccttaa cgtgttgtat
     6601 aaagtgatgc ctttaaacct caatgatatt ctgaattatc ttgcggcaac ctatacaacc
     6661 gggtttttgt cggtagattt gaaaacggtt tcacaacagg catactccga tatggcggat
     6721 aaaataaata tcggggccga ttctgcttct gatagtgaaa tgatgaaaaa agcggaaaaa
     6781 atcacgacac catcccaatc ccagtcgcgc ggccttctgc aaagactgat gaaaaaactg
     6841 ttgggcagct aagaggcgga gagatgagag aaaataaaat tattttcaca ggacctgtcg
     6901 gcgtagggaa aaccactgcc attgcggcta tttcggacga agcactcgtc cagaccgatg
     6961 cttccgcatc cgacatgact ttggatagga aaaggaatac gacagtggcg atggactacg
     7021 ggccatcagc ttggatgagg ataccaaagt ccatttatat agtacgcccg gtcaggaacg
     7081 gttcaacttt atgtgggaaa tcttaagcca aggcagtatg ggtttggtat tgcttttaga
     7141 taatgcccga accaacccgt tgaaagattt ggaattcttt ttacattcgt ttcgagggct
     7201 gctggagaag gcatccgtcg ttgtcggtat taccaagatg gatatacgct ctcagcccgg
     7261 tatcgacgtg tatcacaaat atcttgcaaa acataatctt aatgttccgg tttttgaaat
     7321 tgatgcccgt aaggaagatg acgtaaaaca attggttagc gcaatgttat tttctattga
     7381 tccgggactg gaggtttaat atggaatcaa cactttcact acaagcgaat ttatatccct
     7441 gcctgactcc tgccggtgca ttttatgccg tatccagcga tgcccccagt gccggtaaaa
     7501 ctttgttgcg cagcctgttg aaagcggatg cggacgaagt ggtcagcagt gagaagctgc
     7561 tcgcgggcgg acaccgccga catcgatacc gctttgaacc tgttgtaccg tttgcaaaaa
     7621 ctcgaattcc tctatggcga tgaaaacggt cattcagacg gcatcaattt gtcggacgag
     7681 caattgccgt tgctgatgga acaattgtcc ggcagcggta aggcattatt ggtcgatcgg
     7741 aacggtctgt atcttgccaa cgccaatttc catcatgagt cggcggaaga gttggggttg
     7801 ttggcggcag aagtcgcaca gatggaaaag aaataccggc tgctgattag gaacaacctg
     7861 tatatcaaca ataacgcttg gggcgtttgc gatccttccg gtcagagcga attgacattt
     7921 ttcccattgt atatcggttc aaccaaattt attttggtta tcgccggcat tcccgatttg
     7981 agcaaagagg catttgttac tttggtaagg attttatacc gccgttacag caaccgcgtg
     8041 taaaacttgg gacaggaggg attatgcagc aattattgat ttcaatcctt gaagatttaa
     8101 ataatacatc tacggatatt atcgcgtctg ccgttatctc aaccgacgga ttgccgatgg
     8161 cgacaatgct tccttcacat ttgaattcgg acagggtagg ggcgatttct gccactttgc
     8221 ttgctttggg gagtcgctcg gtgcaggaac tcgcctgcgg ggaattggaa caagtgatga
     8281 ttaaaggaaa atcaggctat atccttttaa gtcaggcggg taaagatgcc gtgttggtgc
     8341 tggtggcaaa agaaaccggc agacttggtt taatcctatt ggatgccaaa cgtgcggcaa
     8401 ggcatattgc ggaagccata taacatataa agattgcggg cttgcagata agtgcaatcg
     8461 attgtcaatt tatattgaca cgttcggtat ttctgtttta ttattcgcgt ttactccccg
     8521 atagctcagt cggtagagcg acggactgtt aatccgcagg tccctggttc gagcccaggt
     8581 cggggagcca aattccaaaa ccctctaagt attttcttag agggttttgt tttaccggcg
     8641 gtcagaaacg catttttgag atgattgttt tgagatggaa taaaatcttt gcaaaattcc
     8701 tttcgtgatg gttatgaaaa ataaatttct aaagatttaa ttggttattt ttataatttt
     8761 gaaatttatc ggttttgtaa aggtctcggg ataagcaatt tcggtcttcg gcgcgtcttt
     8821 tacgccaaac ccataagaat tttgagggta atcttcatcg ttttgcctga attgccgacg
     8881 caaagcgatt tatcggcggt tgtgtttgag cgtttgattg cagcttgccc aatctcaaat
     8941 atttctaaat accaattgaa attcaatatt ttataaaaac actgatttgg aacgaacaga
     9001 tttataaaaa ttccgtgtat ccttgagtaa gggagttgat tgaaaagact tgtttgattg
     9061 atgatgaaga ataagttaat taatgtctta tttggtattc gttctattca tattttttcc
     9121 cagttccttc actaaaaaat ccaaaaatac gcgtaagcgg aggttgacgg ctttatcgct
     9181 gtaataaaca gcattaaagg ggtgtgtttt attggaggtt tgttcggcga ataggggaat
     9241 taactttcct tcagtgatgt cgttgtcaac caaaaaatct gataagcaag caataccgca
     9301 acttgaaagg cacaacgagc gtaagatttc accgctgctg gcggtaaagt gcggtgaaat
     9361 tttataggga tttccctgcg catctaaaac cgcccatgta tttagagaac cgggttctgt
     9421 gaagcctaaa cattgatggt tggcaagatc ttctgcagat tgtggcgtac cgtgttttgc
     9481 taaatattca ggactggcga ctacgcggaa gtggctgtca aacagatggc gtgcacgcag
     9541 cccggaatcg tccaattctc cggcccgtaa ggcaatatcg actttgcgtt caatcagatt
     9601 gatatagcct tcggaagaaa cgagcgaaag tcggatatgc ggatagcgtt cgttgaattt
     9661 tgctgccagc ggcgccagca gatgcagcac catcggcatc gcggaatcca cgcgcaacac
     9721 gccttgcggt acttcgtgca ctgccagcat ttcggtttcc gccgctgcca tttcttgcag
     9781 gattctctgc gcgcggcgga aatattgcgc gccttcttcc gtcagattga gttgccgcgt
     9841 ggtgcggttg agcaggttca cgcccaactt ttcctccagc cgtttgacga tgcggcttac
     9901 ggcagaattt gccatctcca actgctccgc cgcacggctg aagctgccgc tttccaccac
     9961 ttgaacaaat acggtcagtt cttctgaatt ggttttcatc gtgtttcctt ttcggttgga
    10021 accccaccct ttagggcggc aggatcagac tttatttggg aagggcgtaa ccccttccga
    10081 atcaggacgg cacataggga gacgatttat gtgtcatccc gtgtgttgaa acatctgatt
    10141 attccatttg acgcaaaagt gttttcttat ttttgcactt ttaaatcata aagtaaaacg
    10201 gtacaataca ttcatcaatt cacaaacgag gtaacaaatg aatattttat tattagacgg
    10261 cggcaaagcg ttcggacatt ctcacggcgg gttaaaccac acgcttcaca aaaaagcgaa
    10321 agaagttttg accgcgctcg gacacaatgt ttaagaaacc gtgattgatg ccggctatga
    10381 tgttgaggcg gaaatcgaaa aattcgtttg gatggatgct gtgatttggc agatgccggg
    10441 ctggtggatg cacgagcctt ggacagtgaa aaaatatatg gacgaagtct ttaccggcgg
    10501 acacggcaaa ctctaccaaa gcgacggcag acaccgcgtc aatccgactg agggctacgg
    10561 cacaggcggc ttgttgcaag gcaaaaaaca tatgctttca ctgacttgga atgcgccgat
    10621 tgaagccttt acccgcgaag gcgatttctt tgaaggcaaa ggcgttgatg ttttgtatat
    10681 gcacttccac aaagccaacg agttcatcgg cttgagccgc ctgccgacat tcttatgtaa
    10741 cgatgtggtt aaaaatccgc aagtggaaaa atacttggca gattatcagg cacacttgga
    10801 aaaagtgttc ggctaattaa aaatccatct tcaacacgga gatggatttt gtttgtttcg
    10861 ttgattttgt gtcagtttca gatgtgggtg cttatccgga cgggtcgtct gaaaatgttt
    10921 gccccaatgc aaaaaaatca ctgcaaacct tcacaaacgg ggctggtagt gattttttca
    10981 aatcaaacag attgaaaacc tgcgccgaat tgttcagacg gcattatttt ttcaattccg
    11041 ccaaaatccc gtcaacggtt ttcttcgcgt cgccgaaaca catcacgctg ttttcgttga
    11101 agaacagcgg gttttgtacg cctgcgtagc cggtgttcat cgagcgtttg aatacgacga
    11161 cttcttttgc cttccatact tctaaaaccg gcatacctgc aatcgggctg ttcggatcgg
    11221 tttgggcggc agggttgacg gtatcgttcg caccaatcac caagaccaca tcggtttcgg
    11281 ggaagtcgtc gttgatttcg tccatttcca aaacaatgtc gtaggggact ttggcttcgg
    11341 cgagcagtac attcatatga ccgggcaggc ggccggcgac ggggtggatg ccgaagcgta
    11401 cttcgatgcc gtttttacgc aaaagctcgg tgatttcggc aacggggtat tgtgcctgcg
    11461 ccactgccat gccgtagccc ggggtgataa tgacgctgct tgcgccttcc agcatttcgg
    11521 caacatcggc agctttgact tctcggtatt cctctatctc ttggctgccg gaagacgata
    11581 tgccgctgtc gctgccgaaa ccgccggcaa tcacggaaac aaacgaccga ttcatggctt
    11641 tgcacataat gtaggacaga attgcgccgc ttgagccgac cagcgcgccg gtaacgatga
    11701 gcaggtcgtt ggagagcata aagcctgccg ctgcggccgc ccagccggag taggagttga
    11761 gcatagatac gaccacgggc atatccgcgc cgccgatgga ggcaaccaag tgccagccga
    11821 atgcgagggc gatcagggtc ataatcagaa ggatgaagcc gctgccgtca atgccgacaa
    11881 atacgagcag caacacaaac gatacggcga gcgccagtgc gttgagcttg tgtttggcgg
    11941 gcagttgcag cgggctgctg ctgattttgc cgttgagttt gccgaatgcg gccagtgagc
    12001 cggtaaaggt taccgcgccg atgaaaatgc ccaaatagac ttcgaccaga tggatggtgt
    12061 gcatatcgtg cgaaacgttg cccggctcga tgtagctgtt gaagccgacc aaaacggctg
    12121 ccaagccgac gaagctgtgc agaagtgcaa tcagttcggg catttcggtc atttctactt
    12181 ttttggcttt gtggatgccg attgccgcgc cgatgagcat ggcgatgata atccagccca
    12241 gtccgtgggt attgtcggaa aagaccgtta cgaagagggc gaccgccata ccggcgatac
    12301 cggaatagca gccccgtttg gcggtttcct gtttggacag ccccgccagt gagaagatga
    12361 ataaaattgc ggcaacgata tacgccgctg ttacgagtcc tgaagacatg gaaattctcc
    12421 gattttcgat gatttgtttt caatgccgtc tgaaaaattg acgttcgtgt tttcagacgg
    12481 catctgtttc aagcagccgc gacaaacagt ccgacggtgc aggcaaatcc gcccgcccac
    12541 atcagcgagc gcaatgctgc tttgtctgcg atgtagcacc agataaaggc gaggcggaac
    12601 aggatgaaca atccggcaag cgtgttgacg gttgcttgtc cggcattgcc ggttgcgtgt
    12661 gccgtcaaaa cggcggcggc aaacggtgca aaggcttcaa aaccgttttg ctgcgcggcg
    12721 tgggcacggg cggctgcgcc ttgcgtatgt gccagaaaac cgcgaggatt gtggttgtct
    12781 ttaaaccgga atccgcccgc ttttttggca tacgccgcac aaaaaagcgg caataggcag
    12841 gcaatcagaa tgcaccaata ggcgaaagtc atggcttacc ctttcttaaa catattcagc
    12901 atacgccgtg taaccgcaaa gccgccgaag atattgatgc cggcaatcag gatggcaaca
    12961 aacgacagca gcgaaacgaa gccgttgccc tgaccgattt gcagcagcgc gccgacgacg
    13021 atgatgccgg agatggcgtt ggttaccgac atcagcggtg tgtgcagcga gtggctgacg
    13081 ttccaaacga catggtagcc gatgacgcag gcgaggacga agacgataaa gtggttcaag
    13141 aatgctgcgg gtgcgaccgc gccgacccac agcaccaata cggcggcgat ggcggcgggc
    13201 gcgagttttt tccacagggg aacaggtttc ggctcgggct tggcggcagg cgcggctttt
    13261 tcagacggcg tttgctgcgg ctgggcggaa acctgaatcg gcggaggcgg gaaggtgatt
    13321 tcgccgtcgc gggtaacggt catattgcgg ataatcacgt cttcgaagtc cagcgtgatt
    13381 tcgccgtctt tgttcgggct taacagcttg gtcaggttca ccaagttggt ggcgtaaagc
    13441 tgggaagact gtccggcaag gcggtttgcc atgtcggtgt agccgatgat tttcacgccg
    13501 ttgccggtta cggacaattc gcccggtcgg gtgagttcgc agttgccgcc cgtcgccgcc
    13561 aaatcgacga tgacggatcc ggatttcatg ctttccacca tttctttggt aatcagcttg
    13621 ggagcgggtt tgcccggaat ggcggcggtg gtgatgatga tgtccacttc tttcgcctgt
    13681 tcggcaaaga gcttcatttc ggcggcgata aattcgtcgc tcatcacttt ggcgtagccg
    13741 tctccgctgc cgcccgattc ttgcaggaag tcgagtttca ggaacttacc gcccatcgat
    13801 tcgatttgtt ccgccacttc caagcgggta tcgaacgcgc gcaccactgc gccgagcgaa
    13861 tttgccgtac cgattgccgc caaacccgcc acacctgcac caatcaccaa aacctgcgca
    13921 ggcggcactt tgccggcggc agtgatttga ccggtgaaga aacggccgaa ggcgttggcg
    13981 gcttcaatca cggcgcggta gccgctgatg tttgccattg aagacaaagc gtccaaggcc
    14041 tgagcgcggg aaatgcgggg aaccatgtcc atcgccagcg cgttgacttt cttggcgcgc
    14101 aaggcctcga ccaaagcctc gttttggcgc ggccacagga agctgacgat ggtttgacct
    14161 tctttgagca gcggcagctc gccttcggac ggcgcgttga ccttataaat taaagggcag
    14221 gcccaaaccg ccgctttgtc ggcaacggtt gcgcctgctg tttggtaagc ggcatcgtcc
    14281 aaacttgccg ccaaacctgc accgctttcg acaacggttt caaagcctag tttgcccagc
    14341 agggcaacgg tggcgggcgt gcaggcgacg cgggtttcgc cggataatga ctcgcgtggg
    14401 ataccgattt tcatctctga atcctttttc gggttgttca tatgtatcgt gggttaaatt
    14461 taaatcggtg cagggcggag caacgccgta ccggtttaaa accgattcac ttcaaatgtt
    14521 aatatatttt agataatccc cttataacga attttcatca ggctggcaat agttgcgcat
    14581 tttcccgtgt tgtccgacac atactgccgt ttgcgtttga agtcgttttc cgattgcagg
    14641 cacgcccgcc gtaacattct ttgccgtttc actatataat ccgcattttt tgagccgccg
    14701 ttatgccgca agccctcgtc ctccaatttc cctccgccga agccctgcct tccgacttcc
    14761 cctcacgcct gcccgaacct gattatgctg atgaaaagcg tatgcgtttt attgttgaag
    14821 aaggattttc tttaagcgaa aaagacgcgg cgttactgga cagccgtcaa atcgaccacg
    14881 ccgttttgcc caatatggcg tttggcgaac tcgggctgat tgtcagcgat atggattcga
    14941 cgctgattac catcgaatgt atcgatgaaa ttgcggcagg tgtgggtttg aaaggcaaag
    15001 tagcggaaat taccgagcgc gccatgcgcg gagagttgga tttcggacag tctttacgca
    15061 gccgcgttgc gttgttggcg ggattggacg aacagatttt ggcggacatt tatgaaaacg
    15121 ttttgaagct ctcgcccggt gcggaatttt tgttggacga atgcaaaagg cacaatgtga
    15181 aattcctgct ggtgtccggc gggttcacgt tttttaccga aaggctgcaa caacggctcg
    15241 gcttcgaata ccaacacgcc aatattttgg aaattgaaaa cggcaggttg accggccgtc
    15301 tgaaaggcag aatcatcgac gcgcaggcaa aggcggattt attgcgcgaa taccgcagcc
    15361 gcctcggatt gcagccgcat caggtgttgg cgatgggcga cggtgcgaac gatattccga
    15421 tactcaaaga agcgggcata ggcgtggctt accgcgccaa accgaaagcg cgggccgccg
    15481 ccgatgcctg tatcaacttc ggcggtttgg agcgtgtacg cggcctgttc ggataggcgg
    15541 ataggaaacg gatgccgtct gaaaggtttt cagacggcat ttgaacggca ggaacgacag
    15601 tgggacgcag aaaactttgg tttgccctgg cggcagcagg cgttatcggc ggtttggtcg
    15661 gcattgtgct gacggaactg atgcacttca tacggcatac ggcatacggt tatgacgcgg
    15721 acggtgcgta catctcgttc cgcgaaggtg tggcacaggc tttcggtatg cggcgcgttg
    15781 ccatgctgac gctgtgcggc gcgatcgcag gcggcggctg gtggctgctc aaacgtttcg
    15841 gcaagccgca aatcgaaatc aaagccgcct tgaagcagcc gttgcagggg ctgccgtttc
    15901 tgacgacggt tttccatgtt ctgctgcaaa tcataacggt cggactcggt tcgccgctcg
    15961 gacgcgaagt cgccccgcgt gaaatgactg ccgcgtttgc ttttgccggc ggcaggcggt
    16021 tgggtttgga cgaaggcgaa atgcgcctct tgattgcctg tgcgtccgga gcgggtttgg
    16081 cggcggtgta taacgtgccg ctcgcctcca cgctgtttat cctcgaagcg atgttgggcg
    16141 tgtggacgca gcaggcggtt gccgccgcat tgctgacttc cgtcatcgcc accgccgtcg
    16201 cgcgcatcgg cttaggcgac gtgcagcagt atcatcccgc caaccttgcc gtaaatactt
    16261 cattgctttg gttttccgcc gtcatcggcc cgatacttgg cgcaaccgcc gcctttttcc
    16321 ggcgcaccgc ccaaaaattc cccttcatca agcgcgacaa tatcaaaatc attcccttgg
    16381 ccgtctgtat gtttgtactc atcggtgtga ttgccgtttg gtttcccgaa attttgggca
    16441 acggcaaagc aggcaaccaa ctgacttttg gcggtttgac cggctggcaa tacagccttg
    16501 agttgaccgc tgtcaaatgg gcggtcgttt tgatggcgct ggcggcaggc gcatacggcg
    16561 gactgattac gccgtctatg atgctcggca gcaccatttc ttttgcagcc gccgccgcgt
    16621 ggaacagcgt cttccccgaa atgccgtctg aaagcgcggc agtcgtcgga gcggcggtgt
    16681 ttctcggcgt atcgctcaaa atgccgctga ccgccattgt gttcgtattg gaactgacct
    16741 acgcgccgct gtccctgctg ctgcctttgt gcatcggcat ggcggggcat tgggaacatc
    16801 gggaaaaatg ggcttcaaat gaatgccgac aaatgccgtc tgaaaggttt ccagacggca
    16861 tttctttgca aattgtcaac aatatctgca aaaacctact gccaaaaaac tgaaaatagc
    16921 ggataatacc gccccgaaaa agatcccctt tcaccatcca accgtcagaa aaggaagccc
    16981 caatgtccgt catcaaacgc gccctgatca gcctgtccga caaggcaggc gcggttgaat
    17041 ttgcccaaaa cctgcacaag ctcggtgtcg aaatcctctc caccggcggc acggcgaagc
    17101 tgcttgccgg tgcgggcgtt cccgtgattg aagtcgccga ctataccggt ttccccgaaa
    17161 tgctcgacgg ccgcgtgaaa accctgcatc ctaaaattca tggcggcatc ctcggtcggc
    17221 gcgatttgga cgaacacgtc gccaagatgg aagaacacgg catcggcaac atcgaccttg
    17281 tgtgcgtcaa cctctacccc ttcgccgcca ccatcgccaa gcccggctgc acactggaag
    17341 acgcaattga aaacatcgac atcggcggcc cggccatggt gcgttccgcc gcgaaaaact
    17401 ggaaacacgt cgccatcgtt accgacaccg ccgacttccc cgccattgcc gccgaactcg
    17461 aagccggcaa cggcgcgttg agcgataaaa cccgcttcaa cctctcacgc aaagcattca
    17521 gccacaccgc ccaatacgac ggcatgattt ccaactacct gacctccctt tcagacgacg
    17581 tcttgagcgg ccagccccaa atcggcgaat tcccgagcca gttcaaccaa agctggatta
    17641 aagtgcaaga catgcgctac ggcgaaaacc cgcatcagcg cgccgcgttt taccgcgata
    17701 ttgaccccgc cgcaggcagc ctttccgcct acaatcaact gcaaggcaaa gaattgtctt
    17761 acaacaacat cgccgatgcc gatgccgctt gggaagccgt caaatccttt gacgcgcccg
    17821 cctgcgtgat cgtgaaacac gtcaatccgt gcggcgtagc ggttgcagcc gataccttga
    17881 ccgcctacaa actcgcctac gccaccgaca ccaccagcgc gttcggcggc atcatcgcct
    17941 tcaaccgcga agtcgacggc gaaaccgtca aacagattac cgacaaccaa tttatggaag
    18001 tcctcatggc gccgaagttt accgccgaag ccctcgaaat cgccgccgcc aagaaaaacg
    18061 tgcgcgtatt ggaagtgccg ctcaaagcag gtgccaaccg ctttgaactc aaacgcgtcg
    18121 gcggcggact gttggtacaa actcccgaca tctgccgcat cagccgcgcc gatttgaaag
    18181 tcgtctccaa acgccaaccg accgagcagg aatggaacga tttgctgttt gtctggaacg
    18241 tcgcaaaata cgtcaaatcc aacgccatcg tgttcggcaa aggcggccaa acctacggca
    18301 tcggcgcagg ccaaatgagc cgcgtggaca gtacctgtat cgccgcccgc aaagcgcaag
    18361 atgccggtct cgacctcaac ggcgcatgtg ccgcttccga tgccttcttc ccattccgcg
    18421 acggcgtgga cgtgattgcc gaacagggca tcaaagccat catccatccg gcaggctcga
    18481 tgcgcgatca ggaagttttc gacgcggcgg acgaacacgg catcgccatg gcggtaaccg
    18541 gcatccgcca cttccgccat taaggcagat gaataaggca atgccgtctg aagggttttc
    18601 agacggcatt tttgcgctat ttaccgtaat cctgaaatcc cgtcattccc acgcaggcag
    18661 gaatctagat gagaatccat cctgtcagaa atacccgaat cgtcaggaat acccgtgtca
    18721 tcatttccga tacaccgtaa tcctgaaacc cgtcattccc gcgcaggcgg gaatccggat
    18781 ttgtccgcgc ggaaacttat gcgccgccat tcccgcgaag gcgggaatcc gg
//
LOCUS       DAXFHF010000044        18377 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXFHF010000044 DAXFHF010000000
VERSION     DAXFHF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18377
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.044"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFIRM_001390"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFIRM_001390"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC3028996.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..1941)
                     /locus_tag="ACFIRM_001391"
     CDS             complement(1558..1941)
                     /locus_tag="ACFIRM_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC3028997.1"
                     /translation="MYCLRLRRLVLIFVNPLYQTEEGIAQTVVKGKTTRAEVEARFGK
                     RNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCADYDGEGVVRDYRFTHKVEKD
                     ERSVIRDTVGVIRKEAGKSLSQPEK"
     gene            complement(1995..2075)
                     /locus_tag="ACFIRM_001392"
                     /pseudo
     CDS             complement(1995..2075)
                     /locus_tag="ACFIRM_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2278..3606
                     /gene="miaB"
                     /locus_tag="ACFIRM_001393"
     CDS             2278..3606
                     /gene="miaB"
                     /locus_tag="ACFIRM_001393"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC3028998.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            3624..3788
                     /locus_tag="ACFIRM_001394"
     CDS             3624..3788
                     /locus_tag="ACFIRM_001394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3028999.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(3853..5766)
                     /gene="dxs"
                     /locus_tag="ACFIRM_001395"
     CDS             complement(3853..5766)
                     /gene="dxs"
                     /locus_tag="ACFIRM_001395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC3029000.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVATALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(5851..6768)
                     /gene="xerC"
                     /locus_tag="ACFIRM_001396"
     CDS             complement(5851..6768)
                     /gene="xerC"
                     /locus_tag="ACFIRM_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC3029001.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6962..8026
                     /gene="fba"
                     /locus_tag="ACFIRM_001397"
     CDS             6962..8026
                     /gene="fba"
                     /locus_tag="ACFIRM_001397"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC3029002.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            8165..9023
                     /locus_tag="ACFIRM_001398"
                     /pseudo
     CDS             8165..9023
                     /locus_tag="ACFIRM_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
     gene            9162..9839
                     /gene="tsaB"
                     /locus_tag="ACFIRM_001399"
     CDS             9162..9839
                     /gene="tsaB"
                     /locus_tag="ACFIRM_001399"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC3029003.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            9836..10276
                     /gene="rimI"
                     /locus_tag="ACFIRM_001400"
     CDS             9836..10276
                     /gene="rimI"
                     /locus_tag="ACFIRM_001400"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC3029004.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKNGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            10270..11007
                     /locus_tag="ACFIRM_001401"
     CDS             10270..11007
                     /locus_tag="ACFIRM_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3029005.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPDLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            11069..11710
                     /gene="pyrE"
                     /locus_tag="ACFIRM_001402"
     CDS             11069..11710
                     /gene="pyrE"
                     /locus_tag="ACFIRM_001402"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC3029006.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            11778..12206
                     /locus_tag="ACFIRM_001403"
     CDS             11778..12206
                     /locus_tag="ACFIRM_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC3029007.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            12203..13513
                     /gene="argA"
                     /locus_tag="ACFIRM_001404"
     CDS             12203..13513
                     /gene="argA"
                     /locus_tag="ACFIRM_001404"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC3029008.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            13609..15624
                     /locus_tag="ACFIRM_001405"
     CDS             13609..15624
                     /locus_tag="ACFIRM_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC3029009.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(15737..16693)
                     /locus_tag="ACFIRM_001406"
     CDS             complement(15737..16693)
                     /locus_tag="ACFIRM_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3029010.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            16929..17900
                     /locus_tag="ACFIRM_001407"
     CDS             16929..17900
                     /locus_tag="ACFIRM_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3029011.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHSD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            17933..18256
                     /locus_tag="ACFIRM_001408"
     CDS             17933..18256
                     /locus_tag="ACFIRM_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3029012.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLVDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         4124 a         5600 c         5019 g         3634 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgcggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttccacttt atgtgtaaag cggtagtccc ggacgactcc
     1681 ctccccgtcg taatccgcgc accattccca atgccgccgc tccgatttca tatagatgaa
     1741 attggtcggc aaaaaattat aaatcggcag gctgacctcg tgataggcat aacagccgaa
     1801 agggttgcgc ttcccgaaac gtgcctctac ctccgcccgg gtcgttttgc ctttaacaac
     1861 cgtttgtgcg attccctctt ccgtctgata tagtggatta acaaaaatca ggacaaggcg
     1921 acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgctgta ctggtttaaa
     1981 tttaatccac tataacgcag gaactgatgt tccctgtcgc cgaaattgct ggtacacgca
     2041 cacagcagca atgccgccca tacaaccggt ttcatacaca cctcccatta aagccaaaca
     2101 ttatacagcc gttccgaccg attaaattca tattttaaaa caatattttg cctcccaaac
     2161 ccacatcgtg ctataatccg caccgatttt cagacggcat cgtcgtgccg tctgaaattt
     2221 tttcattcca acaaccatca gccccgcgat tacggctgcc tgagaaagac acaaaccatg
     2281 aaaaaagtat ttatccgcac cttcggctgc cagatgaacg agtacgacag cgaaaaaatg
     2341 ctgtccgtcc ttgccgaaga acacggcggc atcgaacagg ttacccaagc cgacgaagcc
     2401 gacatcatct tgttcaacac ctgttccgtg cgcgaaaaag cacaggaaaa agtgttctcc
     2461 gacttggggc gcgtccgtcc gctcaaagaa aaaaaccccg gcctcatcat cggcgttgcc
     2521 ggctgcgtcg cctcgcaaga aggcgaaaac atcatcaaac gcgcgcctta tgttgacgtg
     2581 gttttcggtc cgcaaacgct gcaccgcctg cccaaaatga ttgtggacaa agaaaccagc
     2641 gggctgtcgc aagtcgatat ttccttcccc gaaatcgaaa aattcgacca cctgccgccc
     2701 gcccgcgtcg aaggcggcgc ggcatttgtg tcgattatgg aaggctgttc caaatactgc
     2761 tccttctgcg tcgtccccta cacgcgcggc gaagaattct cccgcccgct caacgacgta
     2821 ttgaccgaaa tcgccaacct tgcccagcaa ggcgtgaaag aaatcaacct cttgggacaa
     2881 aacgtcaatg cctatcgcgg cgaaatggac gacggcgaaa tctgcgactt cgccaccctg
     2941 ctgcgtatcg tccacgaaat ccccggcatc gaacgtatgc gcttcaccac cagccacccg
     3001 cgcgagttta ccgactcgat tatcgagtgc taccgcgacc tgcccaaact ggtttcccac
     3061 ctgcacctgc cgattcaaag cggttccgac cgcgtattga gcgcgatgaa acgcggctac
     3121 accgctttgg aatacaaatc catcatccgc aaactgcgcg ccatccgtcc tgatttgtgc
     3181 ctgagcagcg acttcatcgt cggtttcccc ggcgagaccg aacgcgagtt cgagcaaacc
     3241 ttgaaactgg tgaaagacat tgccttcgac ttgagcttcg tgtttattta cagcccgcgc
     3301 cccggcacgc ctgccgccaa cctgccggac gacacgccgc acgaagaaaa agtgcgccgc
     3361 ctcgaagcct tgaacgaagt catcgaagcc gaaaccgccc gcatcaacca aaccatgatc
     3421 ggcacggttc aacgctgcct ggtcgaaggc atctccaaaa aagaccccga ccagctccaa
     3481 gcccgtaccg ccaacaaccg cgtcgtcaac tttaccggca cgcccgacat gatcaaccaa
     3541 atgatcgatt tggaaatcac cgaagcctac accttttccc tgcgcggcaa aattgtcgaa
     3601 gcctaaaacc ctcacgccga aaaatgccgc ctgaagcgtt tcagacggca ttttgccttg
     3661 tatcggcaga cggcaccgcc gccgttatac ggacaagcgg aataaatttc caaacgcgca
     3721 acggcacacc aagcaccgcc cgaaagccgc cgcccgaacc cttcctgccg cctgcggggt
     3781 ttcaataaaa aaaccgcaga aacaaacgcc gtttaaaaac gccgcccgat aacgacggcg
     3841 cggtcaagcg gtttaatttg ccgcatcacg gtccggcagc cactcgcgca cccggcgttc
     3901 caccgcttcg gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt
     3961 tacggtatcg gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac
     4021 ttccaagacc gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg
     4081 gtcgtggctt cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat
     4141 atcggcgacg gtggcgttca gtttttcggc aaccgccaat gcggtggcga ccatactgcc
     4201 gaaggcaatg aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac
     4261 ggtttccatg ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc
     4321 ggcgggcgca tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct
     4381 cggcgcggca acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc
     4441 atgggtcggg ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag
     4501 gttttgcagg gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata
     4561 aatcgccacg acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg
     4621 ctgctcggcg atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccaccag
     4681 tccgctgccc tcgcgcatgg cgggggtaat cgcaaccagt cgggaatctg ccgccgcccg
     4741 gtcgcacagc catttgccga atacttgggt ataggtcggt ttggcggcgg gcttgggttc
     4801 tttttcagac ggcatttgcg ccccgccttc tttaggcagg ttggcgacgg cgtggtattt
     4861 gacggggtcg ttttcggcga gtttgtagcc gttgcccttt ttggtgatga cgtgcagcaa
     4921 ctgagggcct ttgcggctgc gcaagtcttt caatacgtcc accagattct cgacgttgtg
     4981 tccgtccacg gggccggtgt agcggaagcc gaaattttca aacagcgaca gcgactgttt
     5041 ggcgtgttcg gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc
     5101 catcgcgccg ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag
     5161 tccgtgcata tcgcgcacga cgttgctggc aagatatttg ggcaacgcgc cgacgttggg
     5221 ggaaatcgac atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcccgc
     5281 gcaattcaag gcttcaaacg cctgccccgc cgtcatcgcg ccgtctccga tgatggcgac
     5341 gctgcggcgg tcgccgccca agagtttgtc cgccgccgcc atgcccaaag ccgcgccgat
     5401 ggaggtggag gaatgcccca cgccgaacgc gtcgtactcg gactcgcaac gtttcggaaa
     5461 acccgccaaa ccgccgtatt ggcgcatggt gtgcatctgg tttttcctgc ctgtcaggat
     5521 tttgtgcggg tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgtcgta
     5581 cacatagtgc agggcgatgg tcagttcgac cgcacccaga ttgctggcga aatgcccgcc
     5641 ggtctgcccg acagattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg
     5701 ttttttgtcc aggcggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg
     5761 gttcatggtg tgtctttttt atgtgtcgtc cgggtgcaac ggtcaattat atatcaagag
     5821 cgtgcggctg acggctgatt ttgccgtacg tcattcgtcc tgccgcttgg cgcgcgggtg
     5881 ggcttcgtca tagaggcggg cgatgtggtc gaaatcgagc ttggtataaa tctgcgtggt
     5941 cgaaaggctg ctgtgtccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa
     6001 caggtggccg gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgta
     6061 ttgcgccgcc cacgattcga ggcgtttttg gatttggcgt tggctcaggc gcgtgccgtt
     6121 cctgccggta aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt
     6181 caaggcttcc acgcttttgc cggtcagcgg aacctgacgc tgcttgcgcc ctttgccggt
     6241 aacgtgtacc cacgcttcgt ccaaatatac atcatctgca ttcaagccgt gtatctcgct
     6301 cacgcgcaaa ccgctgccgt acatcagctc gaacagcgcg tggtcgcgca ccgccagcgg
     6361 gtcgccgccg tccacgggca aatccaacat ccggttcagc cattcctgcg gcagggcttt
     6421 gggtacgcgc tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg cgcgcatcag
     6481 cccgcgtttg accagccagg cgcaatactg ccgccacgcc gacagcttgc gcgccagcgt
     6541 ccgaccgtcc aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcgccgcga
     6601 caagtcctgc ggcacgccgc ctgcatcttc agacggcatt tgtgccaaca gtgcaaacag
     6661 ttcttccaaa tcgcgccggt atgccgcaac cgtgtgctcc gacttgccct cgcgcacgat
     6721 gttttccaaa taagcgtcaa aatacgccgc aaacccgtcc aaaaccataa ccgtcccaca
     6781 caaatatcaa aaaaccagtg aaaacattat cataaacaac acaccaaaat cccgaaccgc
     6841 ccaaaatccg ccccatctgc ccgccgttat cgttaatata aaatcaatga gccgtttatg
     6901 gttttttgcc gtaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca
     6961 aatggcactc gtatccatgc gccaactgct cgaccacgcc gccgaaaaca gctacggcct
     7021 gcccgcgttc aacgtcaaca acctcgaaca aatgcgcgcc attatggaag ccgccgacca
     7081 agtcaacgcg cccgtcatcg tacaggcgag cgcaggtgcg cgcaaatacg cgggcgcgcc
     7141 gtttttgcgc cacctgattc tggcggcagt cgaagaattt ccgcacatcc ccgtcgtgat
     7201 gcaccaagac cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttctc
     7261 ctccgtgatg atggacggct ctttgctcga agacggcaaa accccttctt cttacgaata
     7321 caacgtcaac gccacccgta ccgtcgtcaa cttctcccac gcctgcggcg tgtccgtcga
     7381 aggcgaaatc ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggagt
     7441 gggcgcggca ggcaaactct cacacgacca aatgctcacc agcgttgaag atgccgtgcg
     7501 tttcgttaaa gataccggcg ttgacgcatt ggcgattgcc gtcggcacca gccacggcgc
     7561 atacaaattc acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaggaaat
     7621 ccaccaagcc ctgcccaata cacacatcgt gatgcacggc tccagctccg ttccgcaaga
     7681 atggctgaaa gtcatcaacg aatacggcgg caatatcggc gaaacctacg gcgtgccggt
     7741 tgaagaaatc gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgacct
     7801 gcgcctcgct tccaccggcg cggtacgccg ctaccttgcc gaaaacccgt ccgactttga
     7861 tccgcgcaaa tacttgggca aaaccattga agcgatgaag caaatctgcc tcgaccgtta
     7921 tcttgcgttc ggttgcgaag gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat
     7981 ggcaagccgt tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa
     8041 acaaaatgcc gtctgaacgc cgttcggacg gcatttgatt tttgcttctc tgacctgcct
     8101 cattgatgca gtatgcaaaa aaagatacca taaccaaaac gtttatatat tatctattct
     8161 gtgtatgact aggagtaaac ctgtgaaccg aactaccttc tgctgccttt ctttgaccgc
     8221 cggccctgat tctgaccgcc tgcagcagcg gagggggcgg aggcggtggt gtcgccgccg
     8281 acatcggcac ggggcttgcc gatgcattaa ccgcgccgct cgaccataaa gacaaaggtt
     8341 tgaaatccct aacattggaa gcctccattc cccaaaacgg aacactgacc ctgtcggcac
     8401 aaggtgcgga aaaaactttc aaagccggcg gcaaagacaa cagcctcaac acgggcaaac
     8461 tgaagaacga caaaatcagc cgcttcgact tcgtgcaaaa aatcgaagtg gacggacaaa
     8521 ccatcacact ggcaagcggc gaatttcaaa tatacaaaca ggatcactcc gccgtcgttg
     8581 ccctacggat tgaaaaaatc aacaaccccg acaaaatcga cagcctgata aaccaacgct
     8641 ccttccttgt cagcgatttg ggcggagaac ataccgcctt caaccaactg cctgacggca
     8701 aagccgagta tcacggcaaa gcattcagct ccgacgatgc cggcggaaaa ctgacctata
     8761 ccatagattt cgccgccaaa cagggacacg gcaaaatcga acacctgaaa acacccgagc
     8821 agaatgttga gcttgcctcc gccgaactca aagcagatga aaaatcacac gccgtcattt
     8881 tgggcgacac gcgctacggc ggcgaagaga aaggcactta ccgcctcgcc cttttcggcg
     8941 accgcgccca agaaatcgct ggctcggcaa ccgtgaagat aggggaaaag gttcacgaaa
     9001 tcggcatcgc cgacaaacag tagtatttga aaaatgccgt ccgaacacga taatttaccg
     9061 ttcggacggc atttttgcgc cataccgccc taggacacga cgacacggca ggcaagccga
     9121 tttcactata taatgccgtc tgaaccaacg agaaccacac catgcaagcc gatttcaacc
     9181 gccccgtcct cgccgtcgat accggaactt cctatttgtc gctcgcgctg cgcgccgacg
     9241 gcgaaatccg tctgttccat caggaagtcg gcatccgcca gtccgaactg atcctgccgg
     9301 aaatccgcac cctattccga aatgcgggca ttaccgccgc cgatttgggg gcgattgttt
     9361 atgcgaaagg ccccggcgcg tttaccggac tgcgtatcgg catcggcgtg gcgcaaggtt
     9421 tggcaacgcc gttcgacacg cctttaatcg gcatccccac gctcgatgcc gccgcctcgc
     9481 tgccgccgcc gcaaagctgc atactcgccg ctgcggacgc gcgtatgggc gaagtgtttt
     9541 atgcgtggtt cgacacgctg aaccgccgcc gtttgagcga ttatcaagtc ggtagggcgg
     9601 cagacattgc cctccccgag gggtacgtct tttcagacgg catcggcagt gcgttcgcac
     9661 tggaaaaccg tccgcccttc tcaggcaaac ccgatatgcc gaccgccgcc gattttctcg
     9721 cgctcgccct cggcggcggc tatcccgcaa ctggcgcggc acacgccgaa ctgctctacg
     9781 tccgcaacaa aatcgccctg actgccaaag aacaggcgga acggaaggca cgaacatgaa
     9841 catccgccca gccttaccgt ccgactgcgc cgcactggcc gcgctcgata ccgtctgcaa
     9901 cccgtccgcg tggacgcagc gccaatttga gtccgcgctt gtttcgccgt ccgaacaggt
     9961 tttccttgcg gaaaaaaacg gcaggattgc cgcctttatc gtttggcaga acctgcccga
    10021 cgaatccgaa ctgcacctga ttgccaccgc gcccgaatgc cgccgacggg gcgttgcgtc
    10081 cgccctgctc gaatattggt acgcacatct gcccgaaggc acgcaacgcc tgctgctcga
    10141 agtccgcgca ggcaacgccg ccgcacaggc actgtatacc aaacacggct tcagcattgc
    10201 gggcaggcgg aaaaactatt actgcgccgc cgacgggcaa accgaagatg ccgtcttaat
    10261 ggagaaaata tgttaagcgc gcgctacctc cacctgcacg aggctttggg cttgggtccg
    10321 atgtggctga aacaggccgc cgccgtcctg ccgcccaaaa acacacccgc aaccccggca
    10381 caggcacgtc cccaaaccgt ccgcgccgcc acgatccgcc cttcccaacc ccataacgtt
    10441 caggcgcggc tcgaaacgat gaaagcgttg gaaaccgccg ccgtacatac gcgcaaaccc
    10501 gcgcctgaga ccgaaacgcc cccgcccgac ctttcagacg gcatcgcccc cgttcccgcc
    10561 gcttcgggca tcaccaagct tgccgtcgtc agcctgtgtc cgccgatcga ggatgcggtt
    10621 tacgggcaac tgttccacgg caaagcgggc atcctgctcg acaacatcct caaagccgca
    10681 ggactggatg ccgcctatgt ccacaaaacc tgttgggtga aaaccgccgc cgtcggtaac
    10741 ccgatgccgt ctgaacaggc agtcgcggat gcgctgggtc aaatcgccgg ggaactcgac
    10801 ggctgccgcg cccccgccgt cctattcctc ggacaggcgt ttgtcaacct ggaacggcag
    10861 gcgatgattg aaactttgtg cggcagccgt cccttcttca tcatcgacca tcccgcccgg
    10921 ctgttgcgcc aacccgaact caaagcccga aactggcagg tgttgaaaca gttgaaacgc
    10981 gccttgcggc aaggcggcgg cagttgaagc gcgccgcacg gggcggtaga atcgcaactg
    11041 cgtcccaata tctgacagaa agcacaaaat gactgatttc cgccaagatt tcctcaaatt
    11101 ctccctcgcc caaaatgttt tgaaattcgg cgaatttacc accaaagccg gacggcggtc
    11161 gccctatttc ttcaatgccg gcctcttcaa cgacggcgcg tccacgctgc aactggcaaa
    11221 attctatgca caatccatca ttgaaagcgg catccgattc gatatgctgt tcggccccgc
    11281 ctacaaaggc attattttgg cggcggcaac cgcgatgatg ctggcggaaa aaggcgtgaa
    11341 cgtcccgttt gcctacaacc gcaaagaagc caaagaccgc ggcgaaggcg gcgtgttggt
    11401 cggcgcgccg cttaaagggc gcgtgctgat tatcgacgac gtgatttccg ccggcacatc
    11461 cgtacgcgaa tcaatcaaac tgattgaagc ggagggtgca acccccgccg gtgtcgccat
    11521 cgcgctcgac cgcatggaaa aaggcacggg tgaattgtcc gccgttcagg aagtggaaaa
    11581 acaatacggc ctgcccgtcg cccccatcgc cagcctgaac gatttgttta tcctgttgca
    11641 aaacaacccc gaattcggac agttcctcga acccgtccgc acctaccgcc ggcagtacgg
    11701 cgtagaataa agcatatgcc gtccgaacca cctgccgctt cagacggcat caaaccgaca
    11761 cacaccgaga aaacatcatg cccgcctgtt tctgtccgca ctgcaaaacc cgcctctggg
    11821 tcaaagaaac ccagctcaac gtcgcccaag gcttcgtcgt ctgccaaaaa tgcgaagggc
    11881 tgtttaaagc caaagaccat ctggcaagca cgaaagaacc tatattcaac gatttgcccg
    11941 aagctgtttc ggatgtcaaa ctcgtccacc gcatcggcac gcacgccatt agcaagaaac
    12001 agatttcccg cgacgaaatc gccgatatcc tcaacggcgg tacaaccctg cacgatacgc
    12061 cgcccgcaac cgccgctgcc gcacctgccg ccgcaccgca ggtttccgta ccgcccgccc
    12121 gtcaggaagg gctcaactgg actattgcaa ccctgttcgc acttatcgtc ctcattatgc
    12181 agctttccta cctcttcatc ctatgaacgc gcccgacagc tttgtcgccc acttccgcga
    12241 agccgccccc tacatccgcc aaatgcgcgg cacgacactg gtcgccggca tagacggccg
    12301 cctgctcgaa ggcggcacct taaataagct cgccgccgac atcgggctgt tgtcgcaact
    12361 gggcatccga ctcgtcctca tccacggcgc gtaccacttc ctcgaccgcc tcgccgccgc
    12421 gcaaggccgc acgccgcatt attgccgggg tttgcgcgtt accgacgaaa cctcgctcgg
    12481 acaggcgcag cagtttgccg gcaccgtccg cagccgtttt gaagccgcat tgtgcggcag
    12541 cgtttcagga ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact tcctgaccgc
    12601 ccgtccgata ggcgtgattg acggaaccga tatggaatac gcgggcgtta tccgcaaaac
    12661 cgacaccgcc gccctccgtt tccaactcga cgcgggcaat atcgtctgga tgccgccgct
    12721 cgggcattcc tacggcggca aaaccttcaa tctcgatatg gtgcaggccg ccgcttccgt
    12781 cgccgtctcg cttcaagccg aaaaactcgt ttacctgacc ctttcagacg gcatttcccg
    12841 ccccgacggc acgctcgccg aaaccctctc ggcacaggaa gcgcaatcgc tggcggaaca
    12901 cgccgccagc gaaacccgac gactgatttc gtccgccgtt gccgcgctcg aaggcggcgt
    12961 gcatcgcgtc caaatcctca acggggccgc cgacggcagc ctgctgcaag aactcttcac
    13021 ccgcaacggc atcggcacgt ccattgccaa agaagccttc gtctccatcc ggcaggcgca
    13081 cagcggcgac atcccgcaca tcgccgccct catccgcccg ctggaagaac agggcatcct
    13141 attgcaccgc agccgcgaat acctcgaaaa ccacatttcc gaattttcca tcctcgaaca
    13201 cgacggcaac ctgtacggct gtgccgcact caaaaccttt gccgaagccg attgcggcga
    13261 aatcgcctgc cttgccgtct cgccgcaggc acaggacggc ggctacggcg aacgcctgct
    13321 tgcccacatt atcgataagg cgcgcggcat aggcataagc aggctgttcg cactgtccac
    13381 aaataccggc gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag acgagctgcc
    13441 cgaaacgcgg cgcaaagact accgcagcaa cggacgaaac tcgcatattc tggtgcgtcg
    13501 cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg ccgcctgaac cccgtttcag
    13561 acggcatttc cccgattata aagacctgcc cgaccctcaa ggaccccgat gaaatcctac
    13621 cccgacccct accgccattt tgaaaacctc gattccgccg aaacgcaaaa cttcgctgct
    13681 gaagcgaatg ccgaaacgcg cgcgcgtttt ttaaacaacg acaaggcgcg cgcactttca
    13741 gacggcattt tgaatcaaat gcaggacacg cggcagattc cgttttgtca ggaacaccgc
    13801 gcgcggatgt accatttcca tcaggatgcg gaatatccga agggcgtgta ccgcgtgtgt
    13861 acggcggcga cctaccgttc cggctatccc gagtggaaaa tcctgttttc ggtggcggat
    13921 ttcgatgagt tgctcggcga cgatgtgtat ttgggcggcg tgtcgcactt ggtggagcag
    13981 cccaaccgcg cgctgctgac tttgaacaaa tcgggcggcg atacggcgta tacgctggaa
    14041 gtggatttgg aagcagggga attggtagag ggcggttttc actttccggc aggcaaaaac
    14101 catgtgtcgt ggcgcgatga aaacagcgtg tgggtgtgtc cggcttggga cgaacgccag
    14161 ttgaccgaat cgggctatcc gcgcgaagtg tggctggtgg aacgcggcaa gagtttcgag
    14221 gaaagcctgc cggcgtacca aatcgataaa ggcgcgatga tggtaaacgc gtggcgttac
    14281 ctcgatccgc agggttcgcc gattgatttg attgaagcgt cggacggttt ttacaccaag
    14341 acgtatttgc aggtgtcgtc cgaaggcggg gcaaaaccgt tgaacctgcc taatgattgc
    14401 gatgtggtcg gctatctggc gggacatctt ttgctgacgc tgcgcaagga ctggcaccgc
    14461 gcgaaccaaa gctatccgag tggcgcgttg gtggcggtga aactgaatcg gggcgaactc
    14521 ggggcggcgc agcttttgtt tgcgcccgat gaaacgcagg cattggaaag cgtggaaacg
    14581 accaagcgtt ttgtggtggc aagcctgctg gagaatgtac aaggccgtct gaaagcgtgg
    14641 cggtttgccg acagcaaatg gcaggaagcc gagttgccgc acctgccctc cggcgcgttg
    14701 gaaatgaccg accaaccgtg gggcggcgac gtggtttatc ttgccgccag cgatttcacc
    14761 acgccgctga cgctgtttgc gctggatttg aacgtgatgg aactgaccgt catgcgcctc
    14821 cagccgcagc agtttgtttc agacggcatc gaagtgcggc agttttgggc ggtgtcgtcc
    14881 gacggcgaac gcattcctta tttccacgtc ggcaaaaacg ccgcgcccga cacgccgacc
    14941 ttagtctatg cttacggagg tttcggcatt cctgaattgc cgcattatct gggcagcgtc
    15001 ggcaaatatt ggctggaaga gggcaatgcc tttgtattgg caaacatccg cggcggcgga
    15061 gaattcggcc cgcgctggca tcaggcggcg cagggaatca gcaaacacaa aagcgttgat
    15121 gatttgttgg cagtcgtgcg tgatttgtcc gaacgcggca tgagttcgcc caaacacatc
    15181 ggcttgcagg gcggcagcaa cggcggcctg attaccgccg ccgccttcgt gcgcgaaccg
    15241 caaagcatcg gtgcgctggt gtgcgaagta ccgctgaccg atatgatccg ttatccgctg
    15301 ctgtccgccg gttcaagttg gacggacgaa tacggcaatc cgcagaaata cgaagcctgc
    15361 aaacgccggc tgggcgaatt gtcgccgtat cacaatcttt cagacggcat cgattatccg
    15421 cccgcactca ttaccaccag cctcagcgac gaccgcgtcc atcccgccca cgcgctcaaa
    15481 ttctacgcca aactgcgcga aacctcgccg caatcttggc tctactcgcc tgacggcggc
    15541 ggccataccg gcaacggcac ccaacgcgaa tccgccgaca aactcgcctg cgtgttgctg
    15601 tttttgaaag aatttttggg ataagggcag ggggcgacac tgccgccgcg aatgaaaaaa
    15661 gatcgtctga aattgctttt tcagacgatc ttttttaatg gttgtttcaa atcaaaatat
    15721 ctatgccgtc ggcccatcag cactttttca catccgaagg caaaaatccg taatgccgcc
    15781 tgaacgcttc gttgaaccgt cccgcgtggc ggtagccgca aaagtgcatc gcggcttgga
    15841 tggtgctgcc cgattcgatg agggcgagcg cgtgttccag ccgcaggcgg cgcaggcgtc
    15901 cggcgacggt ttcgccggtt tgcgctttga aatagcgttt caggtagcat tcgttcagcc
    15961 cgacgcggcg ggcgatttcg gcgatggtca gcgggcgggc gaattcgctg ttcaaaatat
    16021 cggcggcttc gtctatgcgc cggcggcggt agccgttgtc gtggcggcgg aaggtgaagc
    16081 gtaataatcg ggcggagagt tccagcgcgt cggcttcgtc ggcgagcagg ccgaagccgt
    16141 ccgattcgaa cgggcgttgc agcagggggc aggccgccgc cgtcagtgtc cccgcgtttt
    16201 gcgccagccg ttgcagggcg aatcggccta tcgtttgcgg cgaaaacagg cgttcgtcca
    16261 gtaaaccttc gtcgtgccag cggcgcagtt tttccagcga aaaatccaaa tgcagcgcgc
    16321 acatgccgcc gctgtctggc agcagggttt cggatacgtc cgccagttcg ccgcgtacca
    16381 gccagatttc gccggcagac gggcggtatt ccctgccgcc catttgtaac cggttctgcc
    16441 ccgataccat gacgaacaag gcgcagttgt ggctgaaatt gtggatttcg gtgggaaacg
    16501 cacccgttcc gccgccgcgc atccgtgata aggtgatgcc cgagtcgaag cggttgatgc
    16561 acattttcag atgcaaaccg ggctgttttg cctgcgcaat gagtgcgctg tcggaacagc
    16621 cgtccaacgc ccagccggat ttatcggagc ggacataggt ttggtactgg cggtagatgg
    16681 cggcggtgtt catgattgga taggaacgag ttgtctaaca aatgaattaa ataggaatta
    16741 ttaccaataa tcaagcgcag ggattggttg gaacggaaaa ggtcgtctga aagggtgttt
    16801 cagacgacct tttccgtttc gggaatttgt tttgccgtat cgggaatttt gcgttttgcg
    16861 gcgtggtttc tgcaggttgt ttgcttaata ataaacattc ttattcgtat gcaaaggaac
    16921 cgcacaccgt gaaaccgcgt ttttattggg cagcctgcgc cgtcctgccg gccgcctgtt
    16981 cgcccgaacc tgccgccgaa aaaactgtat ccgccgcatc ccaagccgca tccacacctg
    17041 tcgccacgct gaccgtgccg accgcgcggg gcgatgccgt tgtgccgaag aatcccgaac
    17101 gcgtcgccgt gtacgactgg gcggcgttgg atacgctgac cgagccgggc gtgaatgtgg
    17161 gcgcaaccac cgcgccggtg cgcgtggact atttacagcc tgcatttgac aaggcggcaa
    17221 cggtggggac gctgtttgag cccgattgcg aatccctgca ccgccacaat ccgcagtttg
    17281 tcattaccgg cgggccgggt gcggaagcgt atgaacagtt ggcgaaaaac gcgaccacca
    17341 tagatttgac ggtggacaac ggcaatatcc gcaccagcgg cgagaagcag atggagaccc
    17401 tgtcgcggat tttcggtaag gaagcgcgcg tggcggaatt gaatgcgcag attgacgcgc
    17461 tgttcgccca aaagcgcgaa gccgccaaag gcaaaggacg cgggctggtg ctgtcggtta
    17521 caggcaacaa ggtgtccgcc ttcggcacgc aatcgcggtt ggcaagttgg atacacagcg
    17581 acatcggcct gccgcccgtg gacgaatctt tacgcaacga agggcacggg cagcccgttt
    17641 ccttcgaata catcaaagag aaaaaccccg gctggatttt catcatcgac cgcaccgccg
    17701 ccatcgggca ggaagggccg gctgccgtgg aagtgttgga taacgcgctg gtatgcggca
    17761 cgaacgcttg gaagcgcaag caaatcatcg tcatgcctgc cgcgaactac attgtcgcgg
    17821 gcggcgcgcg gcagttgata caggcggcgg aacagttgaa ggcggcgttt gaaaaggcag
    17881 aacccgttgc ggcgcagtag ttttattcaa aacaaaccac aaggagaacc ccatgcaaaa
    17941 cgaaaccatc aacctgaaac agcaccttgc cgccatcaaa gaatactggc agcccgaaat
    18001 catcaaccgc cacgggttcc aattccgcct ggtcaaactt ttggtcgatt acggctggca
    18061 cacgcacgga tacagcgaca aagtgctgtt tgccgtggag ggcgacatgg cggtggactt
    18121 cgccgacggc ggcaacatga cgatacgcga gggcgagatg gcggtcgtgc cgaagtcggt
    18181 gtcgcaccgc ccgcgttcgg aaaacggctg ctcggtggtg ctgattgatc tgcccgaccc
    18241 gtccgaggcc gcctgaaaac gaagtttccg aaggaagctg agtttctgcg aagctaaaag
    18301 cagcctacac cttcaatatg ccaaaagcat agaggattaa caaaaatcag gacaaggcgg
    18361 cgggccgcag gcagtac
//
LOCUS       DAXFHF010000045        18059 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXFHF010000045 DAXFHF010000000
VERSION     DAXFHF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18059
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.045"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..1253
                     /locus_tag="ACFIRM_001409"
     CDS             <1..1253
                     /locus_tag="ACFIRM_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC3029013.1"
                     /translation="KYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASAEG
                     SVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKAGG
                     KTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGDFS
                     TAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHSVS
                     LGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVNDFD
                     AVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFGTY
                     KGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKVLM
                     EQLMQDNRQLKPRLEKLEARLNAM"
     gene            complement(1392..1907)
                     /gene="coaD"
                     /locus_tag="ACFIRM_001410"
     CDS             complement(1392..1907)
                     /gene="coaD"
                     /locus_tag="ACFIRM_001410"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC3029014.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2037..2720)
                     /locus_tag="ACFIRM_001411"
     CDS             complement(2037..2720)
                     /locus_tag="ACFIRM_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFC3029015.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2879..3145)
                     /locus_tag="ACFIRM_001412"
     CDS             complement(2879..3145)
                     /locus_tag="ACFIRM_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC3029016.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3219..3689)
                     /gene="rlmH"
                     /locus_tag="ACFIRM_001413"
     CDS             complement(3219..3689)
                     /gene="rlmH"
                     /locus_tag="ACFIRM_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC3029017.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3742..4128)
                     /gene="rsfS"
                     /locus_tag="ACFIRM_001414"
     CDS             complement(3742..4128)
                     /gene="rsfS"
                     /locus_tag="ACFIRM_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC3029018.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4187..4792)
                     /gene="nadD"
                     /locus_tag="ACFIRM_001415"
     CDS             complement(4187..4792)
                     /gene="nadD"
                     /locus_tag="ACFIRM_001415"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC3029019.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIRKHGLYEK"
     gene            complement(4789..5388)
                     /locus_tag="ACFIRM_001416"
     CDS             complement(4789..5388)
                     /locus_tag="ACFIRM_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC3029020.1"
                     /translation="MSELSEILSYNQKFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5417..6958)
                     /locus_tag="ACFIRM_001417"
     CDS             complement(5417..6958)
                     /locus_tag="ACFIRM_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3029021.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMFKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7349..8734
                     /locus_tag="ACFIRM_001418"
     CDS             7349..8734
                     /locus_tag="ACFIRM_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC3029022.1"
                     /translation="MDGRTQTLSAQTLLGISAAAIILILILIVKFRIRALLTLVIASL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFAPGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASVGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRAIHVPVP
                     ELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPACSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8754..9272
                     /locus_tag="ACFIRM_001419"
     CDS             8754..9272
                     /locus_tag="ACFIRM_001419"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC3029023.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9458..10375)
                     /gene="thrB"
                     /locus_tag="ACFIRM_001420"
     CDS             complement(9458..10375)
                     /gene="thrB"
                     /locus_tag="ACFIRM_001420"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC3029024.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDESLKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10412..11140)
                     /gene="ubiG"
                     /locus_tag="ACFIRM_001421"
     CDS             complement(10412..11140)
                     /gene="ubiG"
                     /locus_tag="ACFIRM_001421"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC3029025.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECVRVEDLAGAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVRPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFISPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11288..12529
                     /locus_tag="ACFIRM_001422"
     CDS             11288..12529
                     /locus_tag="ACFIRM_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC3029026.1"
                     /translation="MSAKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLIVLL
                     YTWFSMLSGGLMILEVNTHYPRGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPVGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPAFKG"
     gene            12681..13208
                     /locus_tag="ACFIRM_001423"
     CDS             12681..13208
                     /locus_tag="ACFIRM_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3029027.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQCRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPRRYGTLLSTT"
     gene            13222..13737
                     /locus_tag="ACFIRM_001424"
     CDS             13222..13737
                     /locus_tag="ACFIRM_001424"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3029028.1"
                     /translation="MRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTELPVKLAV
                     AGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGTRVVLSE
                     NMACTEVMNEEAGFFFSRQNPETLAQAVARAVSLKKQGGHRLSDPMRALNYNPSLSHH
                     IDRLTDMLASV"
     gene            complement(13804..15456)
                     /locus_tag="ACFIRM_001425"
     CDS             complement(13804..15456)
                     /locus_tag="ACFIRM_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC3029029.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLCEHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15599..16162
                     /gene="gmhB"
                     /locus_tag="ACFIRM_001426"
     CDS             15599..16162
                     /gene="gmhB"
                     /locus_tag="ACFIRM_001426"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC3029030.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16192..16935
                     /locus_tag="ACFIRM_001427"
     CDS             16192..16935
                     /locus_tag="ACFIRM_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3029031.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16932..17620
                     /locus_tag="ACFIRM_001428"
                     /pseudo
     CDS             16932..17620
                     /locus_tag="ACFIRM_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
BASE COUNT         4291 a         5457 c         4601 g         3710 t
ORIGIN      
        1 agaaatatgc agtagcatta ggatctagtt ctactgccag tggagagtat tcctatgcgt
       61 cgggctataa ttctgttgcc agtggaaata aatcctatgc tgcaggctat gcttctgttg
      121 ccagtgcaga aggcagtgtt gtaattggcg attcaaggca agtaaaaccg gaagcagatc
      181 aaggggttgc agtaggttct aaagcaacgg taaagaacaa agctaaacaa agggttgtgg
      241 taggttctga ggcaaaggta aatgcagaac gtggtattgc gattggtaaa gaagccaaag
      301 cagggggcaa aacaacgaac acactgttag atggaccggc atattatgcg gacgctattg
      361 ccgtcgggta tcaagcagag gctggcaaag gtggcgccat cgctttggga aaacaagcaa
      421 aagcaacgaa gcaaaatgga atggcacttg gcgtagaatc agaagctgcc ggtgatttta
      481 gtaccgccgt gggtaatgaa agcaaggcaa aggggcaagg cggcgtaggt ctcggtaacc
      541 aatctaaggc agaggcagat tttgcggtgg ctgtggggaa taaggctgaa gcgactaagg
      601 aaaactcact tgtcattggc cgatatgcaa gggcaaacgg aaatcattct gtgagtttag
      661 gatctcgtag tgaaataaaa gatggtgtga gcaattctgt ggcaccgggt tatggttcag
      721 tagctagtga gaataatgtc gtctctgttg cctataaaga aacaccacaa tctacagaac
      781 tttcctatag aaaaattgta ggtgttgatg atggtgtcaa tgattttgat gccgtcaatg
      841 tacgccagtt gaaagcgatg caaggacaaa atatggctga gctattttca gtacgcagcg
      901 aagtgcgtgg tgtggcagcg agtagtgcag ccctatcggc tctgactcca ttatcctatg
      961 atgcaaacaa tcctacgcag tttatggtcg gtttcggcac atacaaagga cgtcaagcta
     1021 tggcattagg attgtcccat ttcgtcaatg accgctttat ggtaaaagta ggcggaacct
     1081 taggcggcaa taagacggga catatgatga atgtaggctt gacttggaaa tttggaacga
     1141 gccaaggtgt tccggctaca agtgataagg tgcttatgga acagttgatg caagataatc
     1201 gtcaattaaa gccacgattg gagaaattag aagcacgtct gaatgctatg tagccttgtg
     1261 gcgataacgg tttattgagt atatcgccaa tgataaagtg cataattttc aaaccgttga
     1321 taatatacaa taaattgtaa ccaatacctc aaacgggttt gaacccgaat atgcctgaca
     1381 ggaactttct gtcaggcatt gttgttgtga tgttctgcaa tcattttttg gtacacggcc
     1441 ggcggcacat atcttttgac ggtttccatc cagccctcgg gcccgaccag tcctttgatc
     1501 atagtggacg acacttcggc gatttcgcgc ggcggcatga ggaatacggt agatatttcg
     1561 ggtgcgaggt cgctgttgat atggcgcatg gagcgttcgt attcataatc ggaggtggaa
     1621 cggatgccgc gcacgatgaa tcccgcatct acctcacggg cgtaatgcac caaaaaccgg
     1681 ttttgaaaca cttcaattct gacgtttggg aagttgtcgg taatggcgca caacatatcc
     1741 tgcctttcag cggcggtata ggtgttgcgt ttgtcgggat tgatgccgat ggcgacgatg
     1801 agttcgtcaa acatagattg cgcctgccgt atcatccaca gatgccccaa tgtgggcgga
     1861 tcgaaactgc cggcataaac ggcgcggcgc ggtgtattcg gtaacatttg attcctcccg
     1921 gcttcatagc cggctgtgtt tttatgtgtt tgcatccgta ttgtatgccc aaaacaaaat
     1981 gccgtctgaa aataagtgct ttcagacggc atggtttgca tggtgtttcg cctacatcag
     2041 tcgtcgccgt ttaaggacag caggatgttg agcaggctgc tgaagatgtt gtaaagcgag
     2101 ataaacagtg tcagtgccgc gctgatgtaa ctgtcttcgc cgccgtcgat gacggtgcgt
     2161 acctgccaca taatcattaa ggaactgaac aagacaaaac cggcggaaat ggtcagggcg
     2221 agtgcgggaa tacccaaaaa cagattggca accacggcga ccatcagaat gaccgcacct
     2281 acggtcagga agcgtccgag cgcgttcata tcgagccggg ttcggcgtgc caatgcggac
     2341 atcgttaaaa agacggcggc ggtcatcgcg gcggcaatgc cgacgatttt cgcaccgtcg
     2401 gcaatatgga gtgcgtattg cagcacgggg ccgatcaata cgcccatacc gaatgtgaat
     2461 accatcagca gggtaacgcc ggtattgctg taacggtttt tttcgatgaa gtggatcata
     2521 ccgtagaaaa acgccaatac gacggcaaat cctatccagc gcgaaccgaa ggcggcgtaa
     2581 aaattgaaac cggcattggc ggcaagtgcc gcgcctgcgg ctgccggaat gaatgaaaat
     2641 ccgagcaggc ggtaggtttt ctgcaggacg gtgtttttag aaaccgtatg cgcggtgtag
     2701 tcgtaaacgt cgtgttgcat atcatcatgc tcctgaaagc gcggttggaa ataacggggg
     2761 attttaacat tgcccaatgt caaaatttgt ccggttgcgt gaagattaaa gttgtccggc
     2821 gtattttaaa ggccgtctga agcagtttcg gacagcctgt gttcaaaacg gaaaaccgtt
     2881 attgcggaac gtatccctgc acggcatccg cgccgtcgcc gaagaaatac tgctccatct
     2941 gctgagccag gtattcgcgc gcgcgcggat cggcaaggct taagcggttt tcgttaatca
     3001 gcatggtttg gtggcgcgtc cacgccgccc atgcttcttg cgatacgttt tcaaaaatgc
     3061 gtttgcccaa ttcgttgggc agcggcggaa atttcatgcc ttcggcttct ttgttgagct
     3121 tgacgcagaa taccattcgt gccatagcgg attcctttgc tgtgttcaga aataacgggg
     3181 cgattttaac cgattagggg gtacagacaa aagccttttt attcccgatg ataaggatgg
     3241 ttgtgcagga tggaaacggc ccggtagagc tgctcggtca gaaggacgcg caccatgccg
     3301 tgcggcaggg tgaggctgga caggcgcatc atcatgcggg cttgttgttt gaggcggtcg
     3361 gtcatgccgt ccgcgccgcc gatgacgaag cagacgtgtt cgccgttttg ccgccagctt
     3421 ttgaggtgtt ccgccagctc gacggaggtc ggtgctttgc cgcgttcgtc aagaacgacg
     3481 aggaacgcgc cttgcggaat ggcttcaagg atgcgttttt cttccgccgc cataccttgg
     3541 acggcattca cgcccgcgcc gcgtttttcg ggtttgattt ctttgagtgc gtaggcggcg
     3601 tcgcgtccga agcgtttggc gtattcggcg acggcctcat caacccaacg cggcattttg
     3661 gtgccgactg ccaagacggt aatgttcaat gctttccccc ttacaggaaa tgccgtatga
     3721 atattcagac ggcatcggga atcagtctgc agcgtgccac ggcttctgca ttccggcgtg
     3781 gaaactcggt ttctcgccgc cccagatggt gtcaatgtcg tagaagtcgc gcacggcagg
     3841 gagcatgacg tggacgacga ggtctcctgc atcaaccaac gtccattcgc cgctgtctcc
     3901 ttcggtactg aggatttcaa aaccggcttc tttcaaatcg acggcaacgt tgttggccag
     3961 tgctttgact tggcgcgtac tgtcgccgct ggcgataatc attctggcaa acagcgaagt
     4021 tttgtcttgc gtttcgagaa cggaaatgtc tttggctttg atgtcttcga gggcattgac
     4081 ggcgaccccg accatttttt gcaggtcttg cagttcttgt tcgttcattg ttttcctaac
     4141 gggatgtttt cagacggcat tatagcctct ccttactgat ttgactttat ttttcataca
     4201 aaccgtgttt tcggatataa cgggcggcgg caggcgggat gccgtctgaa acaccggcgg
     4261 cactcaggtt gcggcggatt tccgttgacg acacattatg catcggggct gacaagatgc
     4321 ggacgctgcc gtcctgaagg gcattgccca gccacgcgtg cagttcgcgc ggggtttggt
     4381 gcaggctgtc gccctgccgc atggcgacgg cgatattggt ttcgcgcacg agcaactgcc
     4441 attttttcca agtatgcagc ttcagcaggc tgtcgctgcc catcagccac caaagttgcg
     4501 cggacgggaa ctgctggcgg aagatttgga cggtatcaaa agtataggtt gcactttctc
     4561 ggacgatgtc gcagtcgctg acggcaaaac gcgcgtcttc tgccgtcgcc agttcgacca
     4621 tggcaaggcg gtcggcggcg gaagcggagg cggcgtcttt gtgatacggg ccgcctgccg
     4681 gcaggaaaac aaccgcgtcc aacccgattt cgtcggcaaa ggcacgggcg atatgaaaat
     4741 gcccgttgtg tatcgggtcg aaagtgccgc cgaacagtcc gattttcttc atgatgtttc
     4801 cattccttcc gataagtcca tgccgtctga aacactgccg tccacaacca gcgtcagttt
     4861 gccggtaacg ggatggatga ccagtccgtg aacggcgata tggcgcggca tcagcggatg
     4921 gttgcggata aggtccaccg tgtggcgcac gctgtcttcg acgttgtcga aaccggtcag
     4981 ccagccgtcg aggtcgacac ctgcataacg cagggtttcg atacggtctt cgggaatccg
     5041 gctttcccgg acgcgcccga ggaattcttc ggcattcagc ccctgcatac cgcaatcgtg
     5101 atgggcgatg accataatct ctctgacctt caattcaaac acggcaacca aaaggctccg
     5161 catcaccgaa ccccacgggt gcgtaaccag cgcgccggca tttttaatca gcttggcatc
     5221 gccgtttttc aaacccaacg cgtcgggcag cagcccgata atccgcgcat ccatacagga
     5281 caaaaccgcc agcccgcgtt cggggtattt gtcggtaaag tatttttcat attctcccga
     5341 ctcgacaaac ttctgattat aggaaaggat ttcgctcaac tcgctcattt tgccgtcctc
     5401 tgaaaaaggg ttcgcattat aacgtttccg tccgttttcc gccttcgccg ttgtccaaca
     5461 gcaggaaaat gcacaccgca aatgccgaca acagcaatgt caacaccatt gcccgcgcat
     5521 aattgtcctc acccgcacgc cccaaatagg cataaatcaa agtcgtcaac gtctgccatt
     5581 ccggacgcga caggaacaag gttgccgcaa attcgcccac acacgtcgcc gccgccaaag
     5641 tcagaccgcg ccgcaacgcc ggtttcaaga gggggaacgt gatacggcat gccgtctgaa
     5701 agccgtttgc gcccaaacct gccgccgccc tgccgtaatc cggcggcagt gcatcccagg
     5761 ccgataaaac atcttttgcc acaaacggat acgccagcag cgcatacatc gccagcagca
     5821 gcggtaacga agccgtccac cccggataaa gcagcagcac gcccgccgaa acacaaaccg
     5881 gcgacaccat aaacggtaaa aacaccagtc cgcgcatcca caccagccgc cgcgccgccg
     5941 ccgcatacac cacacccaaa accgccgccg caaacaccgc cgccgccgaa aagcgcaaag
     6001 tattccacac tgcctgccac gtttcacttt ccattaacac acgccgcgat tcgccggccg
     6061 accacgcttt cacaacaatt gccgacaaag gaaacaggca gcacacggac aacaccgcca
     6121 ccgaaaatgc cagcaataca tattccccca ccgattgagg cggcgacggc atcacggggg
     6181 aaaccgcctt atccgaaacc gcgcgcctgc cgaaccacgc atacagcaac cctgccgccg
     6241 ccgttacccc caacaccagc cacaccagcg ccgaagcccc cgccatatcg agttcgaaca
     6301 taaccaactg gtaaatttcc acttcgaccg tggcataacg gctgccgccc aacagcaatg
     6361 ccagcccgaa ccccgaaaaa caatacagga agacaaggca cacgccgccg gcaagccacg
     6421 ggcgcaaaac gggcatttca atgtcccaaa accgccgcca cgcccccgcg cccaacgtcc
     6481 gtgccgtctg aagccgtgcc gcaggcactt gagcaaaccc ctgatacgcc gccctgacca
     6541 acacgggcag gttaaaaaac acattgccgt acaacaacag atacggcgta tcctgccggc
     6601 cgcgccacaa cagcccgtcc gccccgaaca gagccagcac gcccacgccc gccaccagcg
     6661 tcggcatcac aaacggcagc atcagcaggc gcagcaccaa agcccgcccc gggaacgcca
     6721 gccgcgccag cacccacgcg acaggcacgc ccaaaggcag caccagcaca caggttgccg
     6781 ccgcctgaaa caccgtccac gccaaacgtt tgaacatata ggcatccgac agcaccgcgc
     6841 gccacgccaa accgtcatac gccgccaccg cccacaaagg cgcaacgacc attaccgcca
     6901 aaaaagccga aggcagcagg gaaaaagcac cccgtaccgc ccaacgccgt ccatccatcg
     6961 ccttccccac ttgaaacact gatgttgcga ttgtacccaa aagccccaca taccgtatat
     7021 ttcaatccga ctacgtaccg tatccgcctt cctcccgccg tctgaaatat agcggattaa
     7081 caaaaatcag gacaaggcgg cgggccgcag gcagtacaaa tagtacggaa ccggttcgcc
     7141 cggtgcttgg gcgccttagg gaaccgttcc ctttgagccg gggcggggca acgccgtacc
     7201 ggtttttgtt aatccactat aaatcgttca aataaacagg aatataactt cagacaaaca
     7261 acttaccgcc cgatttgtgc tatcgttttc gcacaactta aaaaaacctg acaattttgt
     7321 acttttatta cagagaaagg ctttacaaat ggacggccgg acacagacgc tgtccgcgca
     7381 aaccttgttg ggcatttcgg cggcggcaat catcctcatt ctgattttaa tcgtcaaatt
     7441 ccgcatccgc gcgctgctga cactggtcat cgccagcctg ctgacggctt tggcaaccgg
     7501 tttgcccaca ggcagcatcg tcaacgacgt actggtcaaa aacttcggcg gcacgctcgg
     7561 cggcgtggcg cttctggtcg gtctgggcgc aatgctcgga cgtttggtag aaacatccgg
     7621 cggcgcacag tcgctggcgg acgcgctgat ccggatgttc ggcgaaaaac gcgcaccgtt
     7681 cgcgccgggc gttgcctcgc tgattttcgg cttcccgatt ttcttcgatg ccggactgat
     7741 cgtcatgctg cccatcgtat tcgccaccgc acggcgcatg aaacaggacg tactgccctt
     7801 cgcgcttgcc tccgtcggcg cattttccgt catgcacgtc ttcctgccgc cccatccggg
     7861 cccgattgcc gcttccgaat tttacggcgc gaacatcggc caggttttga ttttgggtct
     7921 gccgaccgcc ttcatcacat ggtatttcag cggctatatg ctcggcaaag tgttggggcg
     7981 cgccatccat gttcccgttc ccgaactgct cagcggcggc acgcgagaca gcgacccgcc
     8041 gaaagaacct gccaaagcag gaacggtcgt cgccgtcatg ctgattccca tgctgctgat
     8101 tttcctgaat accggcgtat cggccctcat cagcgaaaaa ctcgtgagcg cggacgaaac
     8161 ctgggttcag acggcaaaaa tgatcggttc gacacctgtc gcccttctga tttccgtatt
     8221 ggctgcactg ttggtcttgg gacgcaaacg cggcgaaagc ggcagcacgt tggaaaaaac
     8281 cgtggacggc gcactcgccc ccgcctgttc cgtgattctg attaccggcg cgggcggtat
     8341 gttcggcggc gttttgcgcg cttccggcat cggcaaggca ctcgccgaca gcatggcgga
     8401 tttgggcatt cccgtccttt tgggctgctt ccttgccgcc ttggcactgc gtatcgcgca
     8461 aggttcggca accgtcgccc tgaccacagc cgccgcgctg atggctcctg ccgttgccgc
     8521 cgccggcttt accgactggc agctcgcctg tatcgtattg gcaacggcgg caggttcggt
     8581 cggttgcagc cacttcaacg actccggctt ctggctggtc ggccgcctct tggatatgga
     8641 cgtaccgacc acgctgaaaa cctggacggt caaccaaacc ctcatcgcac tcatcggctt
     8701 tgccttgtcc gcactgctgt ttgccatcgt ctgacagacg gaaaggatag taaatgacta
     8761 cgcattttgt cgttacgggc gtatgcggct gcggcaagac caccgccgcg ccgtccctgc
     8821 agaaacacct cggtcaatgt ccctatgccg aaggcgacga gttccacacc caagccaacc
     8881 gcgacaagat gggcgcgggt attccgctga ccgatgaaga ccgctatccg tggttgggca
     8941 atctgcgcga ctggatgacg caacaggcgc aaaacggcgc ggaccacacc atcgtaacct
     9001 gttccgccct caaacgcggc taccgcgaca ttctgcgcgg agccgaaggc aaagccgcct
     9061 tcatccacct cagtccgccg caagacatca acctcgaacg catgatgtcg cgcaaagggc
     9121 actacatgaa agcagggatg ctcgattcgc aactggaaat cctcgaggaa ctgggcgaag
     9181 gcgaatacgg ggtcaaaatc gccaaccccg gcacgcccga agcggtcgaa gccgatattc
     9241 tgaactgggt tgcctcggaa aacctgcttt gaagcaatat gccgtctgaa gcccggcaca
     9301 ggatgggttt caggcggcat aaacatcggg aacagaatga attacattga tttaaaatgg
     9361 aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtccgaaac
     9421 cctttcagac ggcatttctg tcaaacgccg gacgcactca atccaaactc aacagcaggt
     9481 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaaat
     9541 ccaacaggcg cgacacccaa aaacggatgc agccggcgcg ttgggcggtc gggaaatacg
     9601 ccttttctcc ggcactcaag gggcgcacgc cctcataacc gccgataaac gcctttttca
     9661 acgactcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
     9721 catacataaa attgccccgg caggcataat agaaatcgat gaagcccgat acctgaccgc
     9781 cgtcaagcaa cacattgtct ttaaacaggt cggcgtggat gatgcccgaa ggcagatggt
     9841 tgccgagatt gtccttcagc gcatcgattt cggaacacag cagcgcggca tcgtcttgcg
     9901 acaggacggg cagcagccgg gcgcacgctt ccgtccacca cgcatcgtaa cgcgggtttt
     9961 ccatttccaa agggaaatcg gcggcggcaa ggtgcatttt cgccaacatc gcaccggtat
    10021 ggaaacactg ctcagccgtc ggcagcgcgg tatccgaacc tttcaggcag gcaaccaggc
    10081 aggccggctt gcccgccaaa acggaatcga gccggccgtc tttgcgcgca accggcgcgg
    10141 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
    10201 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
    10261 aattgctgtt ggtaatcccc tgcgcaatgc cctgcaggga aacaaattcc cccaaatcgt
    10321 aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtatagaca gacatatcaa
    10381 ctcacttgct caaaaacggg caaacccgcc gtcagaacgc cggacggcag gcaaacatat
    10441 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg taagtcatac
    10501 ccttcgtatc cgccacatcc aagcccgcct gacggcacat ccgcgccagc tcggcaggcg
    10561 agatgaattt tttccagtcg tgcgtgcctt tggggacaaa cttcaacaga tattccgccg
    10621 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa aacaccatgc
    10681 cgtccggcct gaccagcttg gcacaggcac gcacgatggc ggcaggatcg gggacgtgtt
    10741 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgccccg gcaaggtctt
    10801 ccacgcggac acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
    10861 cggtttccaa cgactgctcc gccatgtcga tacctttgac aaacgccgcg ccgcgccgcg
    10921 ccatgctttc cgcgaggatg ccgccgccac agcctacatc taaaacccgt ttgccgcgca
    10981 aatccgcgtg tccgtcgata taatccagcc gcagcggatt gatgtcgtgc aaggttttga
    11041 actcgcccga cttgtcccac catttgtcgg caatccggct gaacttggcg atttctccct
    11101 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gccccactca
    11161 aaaacccaag attatactat gggacgcaat gccgtctgaa agccttttcg ggcggcgcgg
    11221 cgcaaatacc ttacaaaccc ttacacttta cggcataatg caggctgttt ttcccgagga
    11281 ggcttatatg tccgccaaaa ctccgtcgct cttcggcggc gcgatgatta tcgccggtac
    11341 ggtcatcggc gcaggtatgc tcgccaaccc caccgccacc tccggcgtat ggtttacagg
    11401 ctcgctgatt gtgttgcttt acacttggtt ttccatgctc tccggcggcc tgatgatttt
    11461 ggaagtcaac acccactacc cccgcggcgc aagtttcgac accatggtca aagacctgct
    11521 cggacgcggc tggaacatca tcaacggcat cgccgtcgcc ttcgttttat acctgctcac
    11581 ctacgcctac atcttcgtcg gcggcgacct gaccgccaaa ggcatcggca gcgcagtagg
    11641 cggcaaaatt tcgctcaccg tcggacaact cgtcttcttc ggcatcctcg ccttttgcgt
    11701 atgggcatcc gcacgcttgg tcgaccgctt caccggcgtc ctcatcggcg gcatggtatt
    11761 aacctttatt tgggcaaccg gcggcctggt tgccgatgcc aaaccgtccg tcctcttcga
    11821 cacccaagcc cccgtcggca ccggctactg gatttacgcc gccaccgccc tgcccgtctg
    11881 cctcgcttcc ttcggcttcc acggcaacgt ttccagcctg ctcaaatact ttaaaggcga
    11941 cgcgcccaaa gtggcgaaat ccatctgggc aggtacgttg gttgccttgg taatttacgt
    12001 cctctggcaa accgccatcc aaggcaacct gccgcgcaac gagttcgccc ccgtgattgc
    12061 cgccgaaggg caagtctccg tcctgattga aaccctgtcc aaattcgccc aaaccggcaa
    12121 tatggataaa atattgtccc tattttccta catggcaatc gccacctcgt ttttaggcgt
    12181 aaccttaggc ctgttcgact acatcgccga catcttcaaa tggaacgaca gcatgtccgg
    12241 ccgcaccaaa accgccgcgc tgaccttcct gccgcccctg atttcctgcc tgctcttccc
    12301 caccggcttc gttaccgcca tcggctgcgt cggcctggcg gcaaccgtct ggacaggcat
    12361 catccccgcc atgctgctct accgttcgcg caaaaaattc ggcgcaggca aaacctataa
    12421 agtttacggc ggcttgtggc tgatggtttg ggtcttcctt ttcggcatcg ccaacatcgc
    12481 cgcacaggta ttgagccaaa tggaactcgt ccccgcattt aaaggataaa ggcaaaatgc
    12541 cgtttgaaac ccgccggcgg cttcaggcgg cattgccgca acaaacggca accgtattcc
    12601 ggcacacagc gcattaccct accccctcac gcacaaatcc cgccccgaca ggcgcgggac
    12661 gcaaccataa aggaacaatg atgaagctca aaatagacat tgcaaccaac aacttcaaac
    12721 acggcggcgg cacggaacgc tacacattgg atttggtaaa gggtctgaac agacaaaaca
    12781 tcacaccggc cgtttatgcg acgaaatttg atcacggcat tcccgaatac gccatgatcg
    12841 aaccccatct tgtcgatcaa tgccggacgc tgaaaaaact acgctcgttc ctcttttcaa
    12901 gccggctcgc tcaaaccaga aaaaacagtg ccgccaaact gattgcctgc caccacgccg
    12961 attacgccga cctcctcatc tgcggcggca cgcacttggg ctacctgcac catatggcgc
    13021 aaaaaccgaa cctgctcgac cgcctcgcca tacgccgcaa ccgcagcaac tacgccaccg
    13081 ccaaactgat tgtggcgcat tcccatatga tgcggcgcga actggtcgga ctatacggcg
    13141 ttccccccga aagaatccaa gtcgcccccc ccccccgcag atacggaacg cttctttcca
    13201 caacctagag aaactgccgc cctgcgcgcc aaatacggtt ttgccgacca tgaaaccgtt
    13261 ttcctattcc catcgaccgg ccacacgcgc aaaggcctgg aactgctcgc cgactttttc
    13321 gaacaaaccg aactgcccgt caagctcgcc gttgccggtt caccgcttcc ccgcccgatg
    13381 aaaaacgtcg tcggactggg cttctgcacc gatatgcccg aactctaccg cgccgccgac
    13441 tttaccatta tggcatccct gtacgaaccc ttcgggctgg tcggtgtcga atccgcccta
    13501 tgcggcacac gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa
    13561 gccggattct ttttctcacg acaaaacccg gaaaccctcg cgcaagccgt tgcccgggcc
    13621 gtcagcctta aaaaacaggg cggacaccgc ctgtccgacc cgatgcgggc gctgaactac
    13681 aacccgtccc tgtcgcacca catcgaccga ctgaccgaca tgctggcatc cgtataacaa
    13741 aatgccgtct gaacgggttt tcagacggca ttttcgcaac cgttgcagcc gtgcggcaaa
    13801 aagctaacgg gcaattttca aaacgtcgta cagatagttg cggtaatttc cgaccaactc
    13861 ccggttcggg cagtcttcca accctccgcc gcacaaaccg cgcatcgcca tattcgcacg
    13921 catgaagccg tctttggctt ccaaaccagc cgcctccatc agtacgccgc ctgcaaaggc
    13981 aaggtcgagg aaatcctgcc gctgtacgaa tccgccggca atattgctcc tgacggcaaa
    14041 ctgcgttaca taatccggct gagcgtaatc ccatttcttc ctgacggaca cgccctcaaa
    14101 cggtacctga tgatcgccga aataaccgaa aacaaagggt ttgccgcgtt cgtgcagata
    14161 acggtcgaaa ctttccaccg ctttgtccaa atcggcaatg cgcccgatgt agtcgttaag
    14221 cgcggatacg gttttcgcat tcaaatcggg cgcatccaaa tcaaacacat tgtcggtatc
    14281 cgtccgatac ggcccgtgct ccttcatggt cagcacatat acgaacatcg gctgccgcac
    14341 gttttccaaa tcgggatggc gtttttcgag aatcatccgc gcatactgca tcatttcctc
    14401 actggaaatg tgccacaggt ttttgcccat cggcgcggga tagcccaaat cttgcggctg
    14461 gaacatcaga ttaaagccga aatggtcata tgctgccttg gcattgtagt tgcccttggt
    14521 aaacggcgag agcgccacgc aaaaataacc gtgttcgcac aggttgcgga caaaaccggt
    14581 ctgcaaatgc ggtacgaccg aataaaacac gccgcttgcc aacgcgccga aatctgtgga
    14641 cggaacaccc gccaaaaacg caaattcgga cttccacgtt gcgccgccga aagtatgcac
    14701 gcgcaaaggt gaggaaaata cagtatcttc ctgccgtccg aacattttca aatcgggaat
    14761 tttggcggcg gcaaaatcga aacagtgcgg atcgagcgtc gattccatca gggtaacgac
    14821 aatatcgggc ttttcatccg acatgccgta cggccgggtt tcagacggca tctgcctggc
    14881 aaacgcttcc ccgtcgccct cgaacacggg tacttggaaa aacaccgccc gacaggacat
    14941 cggcaggttc aaaaacacgt cgcgcccgtc gtccggcagc gaatccagcc acgtctttac
    15001 cgcgccgggg tgcttggaaa aatgccgcac cgacacgaac gccgccgcaa acagaaccgc
    15061 gcccgcccaa cgccacggca tacccaaaga atccgcaccg ctccagccga aaaccgcata
    15121 tgccgccaaa gccagcagcc ccgccatacc gataaccgct tccttataat gaaacagcgt
    15181 ttcccaattc cgccagtcgg caatcagcaa aaagtcggaa atcaacagcg gctgtttgta
    15241 ataatggatt ttcagcctgt ggaacagtat cagcaccaca aaccagaccg aagcgaaatt
    15301 caaggcgcgc tgccactgcg ccgtcaacat cagcataccg cccgccaaaa agacaaacag
    15361 cgccgacgcg aaaaaatacg tccagcggta gtgcgacctg acaatcagca gcaccaccgc
    15421 cgttacaaac aaaaatagga aagcataggc aaccatcccg ccccactcct caaaacggca
    15481 tacctgtgcc gttgcgaatt attatcgaaa gcgtgcatta taatgcagca caggcgcatt
    15541 tccaccgata tttcagtata atgccatccc cgaacccgcc caacccaaag gaaccgtgat
    15601 gaaactcatc attctcgacc gcgacggcgt catcaaccgg gaccgcgacg acttcgtcaa
    15661 atccgttgac gagtggatac ctgtcgaagg cagcatggat gcggtggcat tcctgacgca
    15721 ggcaggctgc accgttgccg tcgccaccaa ccaatccggc atcggccgca aatattttac
    15781 cgttcaagac ctcaccgaaa tgcatgccaa aatgcaccgc ctcatccgtc aggcgggcgg
    15841 ggaaatcgac ggcatctggt tctgcccgca taccggcgcc gacggctgca actgccgcaa
    15901 gcccaaaccg ggcatgattg aagacatcct cgaacgcttc aacgcccaag cttcggaaac
    15961 ttggctggtc ggcgacagcc tgcgcgattt gcaggcaatc gatgccgtcg gcggaaaacc
    16021 cgcgctggtt ctgaccggaa aaggcaaaaa aacgctctcc caacacggac acgaattacc
    16081 cggacacaca caggttttcg ataccctgct cgatttctca caatacatca tgcaggaaaa
    16141 cgccgcaccg caagccgact gaacataccg catttccgac aaggcaaaac catgctcatc
    16201 atccgcaacc tgatttactg gctgatactc tgttccagcc tgattttcct ctttcccttt
    16261 atgctgctcg cctcgccttt ccgggacggg gcgcacaaga tggcgcgggt ctgggtcggc
    16321 atcctcaact ggtcgctcaa acacatcgtc gggctcaaat accgcatcat cggcgcggaa
    16381 cacattccgg accgcccctc cgtcatctgc gccaaacacc aaagcggctg ggaaacgctc
    16441 gcgctccaag agatttttcc gccgcaggtt tacgttgcca agcgcgagtt gttcaaaatc
    16501 ccctttttcg gctggggctt gaaactggtc aaaaccatag gcatagaccg caacaaccgc
    16561 cgcgaagcca acgaacagct cataaaacag ggtttggcgc gcaaaaacga aggttattgg
    16621 attaccattt tccccgaagg cacgcgcctt gcgcccggaa aacgcggcaa atacaaactc
    16681 ggcggcgcgc gcatggcgaa aatgtttgag atggacatcg tccccgtcgc cctcaacagc
    16741 ggcgaatttt ggccgaaaaa ttcctttctg aaatatccgg gggaaattac cgtcatcatc
    16801 tgtccgacca tcccgcacgc aagcggcagc gaagccgaat tgatggaaaa atgcgaacac
    16861 ctcatcgaaa cgcagcagcc gcttatttcc ggcgcaggcc cgtttgccgc cgaaatgccg
    16921 tctgaaaccg catgacctcc ctgattcaca ccctttcaga cggcatagaa ctgaccgtcg
    16981 aaatcaaccg ccgtgccaag aaaaacctga ttatccgccc tatcggcaca cataccgtcc
    17041 gcatcagcgt cccaccctgc ttctccgtct ccgctctaaa ccgctggctg tatgaaaacg
    17101 aagccgtcct gcggcggaca ctggcgaaaa caccgccgca caatacggca aaccggctgc
    17161 ccgaacatat ctggttccac ggcgggcggc ttgccccacc gcccatcagg acacgcaaat
    17221 cctgctgatg ccgcctgaaa tccgcgtccc cgaaggcgcg cccgaaaaac agcttgcgct
    17281 gctgcgggac tttttggaac ggcaggcgca cagttacctg attccccgtc tcgaacgcca
    17341 cgcccgcgcc acgcaactgt tccccgcctc ctcctcgctg acctccgcca aaaccttctg
    17401 gggcgtgtgc cgcaaaacca caggcatacg cctcaaccgg cggctggtcg gcgcacccga
    17461 atacgttgcc gactatgtct gcatacacga actctgccac ctcgcccatc ccgaccacag
    17521 ccccgccttt tgggaactga cccgccgctt cgccccctac acgcccgaag caaaacagcg
    17581 gatcaaaatc cacggcaggg agcttttcgc cttgggctga cgcagccccg ggccgacgct
    17641 ttcagacggc atccgtgccg gaacaggcac gcgcccgccc gattcaaacc gcaatgacgc
    17701 tttgccgccg gttcggggca ggatggcggc atccgcgccg tccgcaccgt ttcatttcac
    17761 accgcccttc cgaaacccgc ccgatccgac gtgcggtatg aaacgcttaa gccggcgcga
    17821 agtcttctac tgatttgccc gcgaaaatgc cgtccgaaag gttttcggac ggcatttttt
    17881 tgcgttttcc tggatggcgg cggattcgta aaaggcgggc agggtggatt gcaggatggg
    17941 ttgaggcctg ccaaatccgc caactctacc gaatacgccg ccgtcattcc cgcgcaggcg
    18001 ggaatttggt tttgtcggtg cggaaactta ccggataaaa cggtttctta gattccacg
//
LOCUS       DAXFHF010000046        17948 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXFHF010000046 DAXFHF010000000
VERSION     DAXFHF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.046"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(51..1472)
                     /locus_tag="ACFIRM_001429"
     CDS             complement(51..1472)
                     /locus_tag="ACFIRM_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC3029032.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1831..2190)
                     /locus_tag="ACFIRM_001430"
     CDS             complement(1831..2190)
                     /locus_tag="ACFIRM_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC3029033.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2187..2792)
                     /locus_tag="ACFIRM_001431"
     CDS             complement(2187..2792)
                     /locus_tag="ACFIRM_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC3029034.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2839..3321)
                     /locus_tag="ACFIRM_001432"
     CDS             complement(2839..3321)
                     /locus_tag="ACFIRM_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC3029035.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3525..3622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3833..4933
                     /gene="gcvT"
                     /locus_tag="ACFIRM_001433"
     CDS             3833..4933
                     /gene="gcvT"
                     /locus_tag="ACFIRM_001433"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC3029036.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5080..5619
                     /locus_tag="ACFIRM_001434"
     CDS             5080..5619
                     /locus_tag="ACFIRM_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029037.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRSKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5671..6057
                     /gene="gcvH"
                     /locus_tag="ACFIRM_001435"
     CDS             5671..6057
                     /gene="gcvH"
                     /locus_tag="ACFIRM_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC3029038.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6210..7457
                     /gene="hemA"
                     /locus_tag="ACFIRM_001436"
     CDS             6210..7457
                     /gene="hemA"
                     /locus_tag="ACFIRM_001436"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC3029039.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7704..10009
                     /locus_tag="ACFIRM_001437"
                     /pseudo
     CDS             7704..10009
                     /locus_tag="ACFIRM_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFIRM_001438"
                     /pseudo
     CDS             10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFIRM_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12390..12545
                     /locus_tag="ACFIRM_001439"
                     /pseudo
     CDS             12390..12545
                     /locus_tag="ACFIRM_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12542..13633
                     /locus_tag="ACFIRM_001440"
                     /pseudo
     CDS             12542..13633
                     /locus_tag="ACFIRM_001440"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13691..14587
                     /locus_tag="ACFIRM_001441"
     CDS             13691..14587
                     /locus_tag="ACFIRM_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3029040.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATILLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14584..15414
                     /locus_tag="ACFIRM_001442"
     CDS             14584..15414
                     /locus_tag="ACFIRM_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3029041.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15411..15905
                     /locus_tag="ACFIRM_001443"
     CDS             15411..15905
                     /locus_tag="ACFIRM_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC3029042.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16039..17700)
                     /locus_tag="ACFIRM_001444"
     CDS             complement(16039..17700)
                     /locus_tag="ACFIRM_001444"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC3029043.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4346 a         4637 c         4857 g         4108 t
ORIGIN      
        1 gattatgccg gaatttaaaa tgccgtctga atgttcagac ggcatagcgt ttacagtagt
       61 ttgaaaacga aaaagacgag ggtatgtacg atgaagacgg gtgtcaggaa ggcgaccgac
      121 cacatcatat agccgaagaa agtcggcatc ggtacgccgc gctgttcggc aatggccttg
      181 accatgaagt tcggtgcgtt gccgatgtag gtcagtgcgc ccatgaatac cgaacccata
      241 gaaaccgcca gcagcgaatg aaacagggga cccgtcatta aggcttgggc atcgccgccc
      301 gccatattga aaaacacgag ataagtgggc gcgttatcca agaatgccga caatatgccg
      361 ctcatccaga aatacatcgt attaatcgga tgacctgccg tatcgtgaac cagcgatacc
      421 accccgccca gcgcgcctgc ctcgcctgct ttcagaatgc tcaggacggg gaagatggtg
      481 atgaagatgc cgaggaagag tttgcccact tcggcgatgg gttcaaagtt gaattcgttg
      541 cctgcgcgga cttgtttggg cgtgattgcc atagatacgg cggtcaatgt aatcaggatg
      601 acatcgcgga caaggttttg cagggcgtaa cggctgccga ggatttcaaa tcccgggtgt
      661 tcgggtttcc aaaggccgga cattagaacc gcgccgacca cgcccgaaag caggaggaag
      721 ttccatttgc cgaagatggc gattttttcg ggtttttcct gttgtgccgg cgtatcttgt
      781 gcaatgcttt cctgtttgaa gaaacggttg tcgatgaaat agaaggcggt caacaggaca
      841 gcggtgctaa tcaggacggg ggcgaacata tgtttgaccg tccacatgaa atctacgcct
      901 ttgaggaagc cgaggaagag tggggggtcg cccaaagggg tcaggccgcc gccgatgttt
      961 gcaaccagga aaatgaagaa gatgacgatg tgcacgcggc gggtacggtc ttggttggct
     1021 ttcagcagcg gacgaatcat cagcattgcc gcgccggtcg ttcccatgat agaggcaagt
     1081 gccgtaccga cggcaagcag ggcggtgttg agcttgggtg tgccgttcaa gtcgccccaa
     1141 accaaaatgc cgcctgaaat ggtgtacagg gcaagcagca gcaggatgaa ggggatatat
     1201 tcttcaacga gtgcgtgtgc gacggtatgg ataccggcgg acgcgccaaa aaccaaactg
     1261 aacgggatga ggaagagcaa tgtccaaaag gcggtgattt tgccgtaatg gtgatgccag
     1321 gtatgcgaaa aaaacaaggg acccaatgcg atagacagca aaatcagggc aaagggcaag
     1381 ccccacagca ggtttaggtt tgcgccgtcc gaatctgcgg cgtaaaccga tgccgggaaa
     1441 agcattagtg aaaacagggg taggtggcgc atcgtgtttc ctcgattcaa gcactgcctt
     1501 gcgcggcgca cgggagtgat acaggcttcg tgccgcccgg acataggcgg actgtgctgc
     1561 ctgtttgttt ttgaaaaaag ttttccgatt gattgtaaag taaataatca ggataaacca
     1621 gtttcccaaa ccggaaggcg gcgggaaggc ggattgccgt gcttgggaat atatgttctt
     1681 tttgataaat aattttattt aaaacaaata aatatatgtc tttaataaat aatctgtcga
     1741 aaacgaaaaa taaatttatt ttaacatata tttgcaatga aacaggtttg ccccacccgt
     1801 ttgtttgccc ttatcccttt cagacggcat tcaagattcg ggtctgcgcc acatccatat
     1861 ggcgacaagg gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt ggggaaactg
     1921 ccaaaacatc atcaggcagg atgcggtcat catgctgatg gcgaaaatct tggctttgcg
     1981 cggcactgcg ccgttttgtt cccagttatg aaccatcggg ccgaaatagc ggtgccggtg
     2041 cagccagcgg tgaaagcgcg gggatgcctt tgcccagcag gcggcggaga gtagtacgaa
     2101 cggcgtggtc ggcaacagcg gcaaaaaaat gccgattatc cccaacagca gggagatgcc
     2161 gccgcaggca attaaaagat aacggatcat tttgaaatat ttttcttatt gtgcggataa
     2221 gggcaggatg tgataccgag ttttgcccag ccttcatgtc ccattttttc cagcagggcg
     2281 atattgcgtt cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt ggcaatgctg
     2341 tcttcgcgga tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt gccgatgccg
     2401 tctgaatccg tgccttcaaa ttggaaatag ggatgaaacg gggcgatttg gacgatgcct
     2461 tccaagccgt tttcgacaac ggcggcatcg gcaatgtcga gcatatcgtt gaatacgtcg
     2521 aaatcgggga atagggtcgg gtgaaccagc agggtggttt ccagttcggt ggcgggtgta
     2581 ttgcccagtc gctgcagttc ttcgtccaag tcttccaaaa aaccgtcaag gtgtttggct
     2641 tcgctgatcg cgatgcggac aaggttttta acgtgggggg ctttggcaaa gggacacagg
     2701 ttcagaccga tgacggcttt ttccaaccat tgtccggtgt gttcggcaac agcatcttta
     2761 ttttcggaag tatcgatatt cattattgtc atgtaaatgt gtttgcagag tgcacgtgcg
     2821 ggaaaatcgg gaagggcact attccttcag caggtggttg agcggcaggg aggtggtgtg
     2881 tttgatttct tttaaaacaa agctcgattg cgcatcctgt acgccgtggt gggataaaag
     2941 cgtatccaaa acaaaatggg aaaacgcgtt catatcggta aaaaatgcct gaagcaggta
     3001 gtcggtctcg ccggttaggg cgaagcagct caagacttcc ggccattttc gaaccgatgc
     3061 ggcaaaatct tcccgcgcgt cttttgcttt gcggatggaa acgcggataa atgcctgaag
     3121 acctaagttg acggattccg gagacagcag cgcggcatat tggcggacga tgccggcatc
     3181 ttccaactgc ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac gttcggacag
     3241 ttcgacattg gtcagcctgc cgttttcctg gaggacctgt aagattttaa tatcggtttt
     3301 gtctaaagtg agttggggca tatttgcgtt ccgttttaag gaattcggat tgtctgtccg
     3361 tatgtttgcg gcaatccgca tagatggaga ccatattaac atataaaaag ttataccgtc
     3421 atccgggaca aattttgttt tcggaaaatc atgtgaaaac agaggcggtc ggtttgcatc
     3481 tcttttaaga cggcttgccc aaaccgccga ttcaagacat aatcgggaaa tgtgcaggag
     3541 agtgttacac ccaactacaa tgtaaccacc gaaggcgcag acacccttaa atcgctcagg
     3601 tatcagggac tgcacattga aacaaacaat ctggagagcg gcgttagaat aacgtccacc
     3661 gaaggggaga aggccgtctg aaccaccatt cagacaaccg cgcaaagcag tgagcagact
     3721 ggtttgccat catgcggata cggccgaaaa tctcaggttc aaggacagat agggtcatcc
     3781 gcgcacaggt gcgcgggcgg catctgaaca aaaaatccgg agaaacttga gaatgactgc
     3841 tctgaaaacc accccgtttc atcaagccca tcaagatgca ggcgcgaagc cggtagattt
     3901 tgccggctgg gaactgccca tccattatgg ttcgcaaatc gccgaacacg aagccgtgcg
     3961 caccgacgcc ggtatgtttg acgtatccca tatgctcgtt accgacgtcg tcggagcgaa
     4021 cgccaaagcc tttttccgca aattgattgc caacgacgtt gccaaactcg cttttgtcgg
     4081 caaagccctt tattccgctt tgctcaacga caacggcggc gtgattgacg acttgatcgt
     4141 ttaccgcacc aacgaggccg aaacccaata ccgcatcgtg tccaacggtg cgacccgtga
     4201 aaaagacacg gcacaattcc acaaagtcgg acaagagttc ggcgttgcct tcaatccgcg
     4261 ctacgacctc ggtatgcttg ccgtacaagg tcctaaagcc attgaaaaac tcctgaccgt
     4321 caaacccgaa tgggcggatg tgattcacgg cctcaaaccg ttccaaggcg cggatttggg
     4381 caacgactgg tttgtcgccc gtacaggcta caccggcgaa gacggcgtcg aagtgattct
     4441 gccggatacc gaagccgtcg cattcttcaa agccctgcaa gcagcaggcg tacagccttg
     4501 cggcctcggc gcgcgcgaca ccctgcgcat ggaagccggt atgaacctct acggcaacga
     4561 tatggacgac gacaccagcc cgctcgaagc aggcatgggc cggacggttg atttgaaaga
     4621 cgaaagccgc gattttgtcg gtaaagccgc cttgttggca ttgaaagaaa aaggcgttgc
     4681 cgtcaaacaa gtcggtttgc tgctcgaaaa aggcggcatc ctgcgcgcgc atatggaagt
     4741 gttgaccgac aaaggaaaag gtgaaaccac cagcggcgta ttctcgccca gcctgaaaca
     4801 atctatcgcc atcgcccgcg taccgaaaga ttttgacggc gataccgcca aagtcctgat
     4861 tcgcggcaaa gaagcggacg tgcgcgtact gaagctgccg ttcgtccgca gcggtcaaaa
     4921 acagtttgat taatatcggt tcagacggca tttgtttgaa ttaagccgtc tgaaagcagg
     4981 ttttaaggaa ttgttatttc ctatgaaagc aaattttggc agtttattgt tgttattgat
     5041 gttatcggct tgcgctttgt cgccggcgag cgcggattga tgtttttcga tacagaagga
     5101 aaatcccata ctgcaatatt tatgaaaatc gttggcggca atggaaagat tgccggcgag
     5161 gtggtaatag acggggaata tgctcttgat attgtccgca tgaatgatga tttgagtatt
     5221 gccgtgcaag ataataaggg tagaagccgt gcttccgacc tggaagcatt gtcgcaagcg
     5281 aaatcattta ccctaattgc gttcagccgt tatgcgggca cagttgaagt ggataaagta
     5341 tcggctgtta caggcatttg tgcagattac cgcagcaaag gcctgaaggt tgccgttgca
     5401 gacaatatga ggtccaaaca agaaatcgtt gtattccgtg caagcggcgt aatggcaagt
     5461 aataagcgca agataaacga agcatatttg gattattctg catcctctaa actgccttcc
     5521 gcccaaaagc aagaattgga acaagataaa aacaatatga ttgccgtcca ttacggacgc
     5581 ttggaagaat taatcgcacg aggtatctgc gtacgctaag ttgtgcagat gccgtctgaa
     5641 aataaccccc taccaatgga gaatcaaacc atgagcaaca acatcccaac agaactgaaa
     5701 tacgttgcca gccatgaatg gctgcgcctt gaagaagacg gtaccattac cgtcggtatt
     5761 acccatcacg cgcaagagct gttgggcgac atcgtgtttg tcgagctgcc cgaagtcggt
     5821 gcgaacctcg ccgccgaaga acagtccggt gtggttgagt ctgtaaaagc cgcatccgac
     5881 gtgtatgcgc cgattgcggg cgaagtcgtt gccgtcaacg atgacttgcc aggcgcgccg
     5941 gaaaccgcca acagcgaccc ttacggcgca ggctggttct tcaaaatcaa acctgccaat
     6001 cctgccgatt acgacggtct gctgactgcc gaacaatacg cgggcgaagt ggattaaacc
     6061 gcccgtttgt ccgacggcaa ccgtcggaca aacggaaact gcaccttcag acggcatttc
     6121 tgcggtcgga ggtgcagttt ttttgtccgt gttttaagga tgcagttagg ctataataac
     6181 ggtctatatc catcttcacc gattttttca tgcaacttac cgctgtcgga ctcaatcatc
     6241 aaaccgcacc tttaagcata cgggaaaagc tggcgtttgc cgccgccgcc ctgccagaag
     6301 ccgtccgcaa tcttgcccga agcaatgcgg caacggaggc ggtaatcctt tctacctgca
     6361 accgcaccga gctttactgc gtcggcgatt cggaagaaat catccgatgg cttgccgatt
     6421 accacagttt gccgattgaa gaaatccgtc cgtatctgta cacgctggat atgcaggaaa
     6481 ccgtgcgcca cgccttccgc gttgcctgcg gcttggattc gatggttttg ggcgagccgc
     6541 agattttggg gcagattaaa gatgcggtgc gtgcggctca agaacaggaa agtatggggg
     6601 caaaactcaa tgccctgttc caaaaaacct tttccgttgc taaagaagtc cgtaccgata
     6661 ccgctgtcgg cgaaaattcg gtttcgatgg cttccgcgtc cgtcaagttg gcggaacaga
     6721 tttttcccga catcggcgat ttgaacgtat tgtttatcgg cgcaggcgaa atgattgagc
     6781 tggttgccac ttattttgcc gccaaaaatc cccggctgat gacggttgcc aaccggacgc
     6841 tggcgcgtgc acaggagttg tgcgacaagc tcggtgttaa cgccgaaccg tgcctgctgt
     6901 ccgatctgcc tgccattctg cacgattacg acgtggtggt ttcttcaacg gcgagccagc
     6961 ttccgatagt cggcaaaggc atggtcgaac gcgcattgaa acagcgtcag agtatgccgt
     7021 tgttcatgct tgacttggcc gtgccgcgcg atattgaagc ggaagtcggc gatttgaacg
     7081 atgcgtatct ttatacggtg gacgatatgg tcaacatcgt ccaaagcggc aaggaggcaa
     7141 ggcagaaggc cgccgccgcc gccgaaacgc tggtgtccga aaaggttgcc gaatttgtca
     7201 ggcagcagca gggcaggcag agcgttccgc tgattaaggc cttgcgggac gagggcgaga
     7261 aagcgcgcaa gcaggtgttg gaaaatgcga tgaaacagct tgccaaaggc gcaacggcgg
     7321 aagaggtttt ggaacggctg tccgtccaac tgaccaacaa gctgctgcat tcgccaactc
     7381 aaaccttgaa taaggcgggg gaagaagata aagatttggt tcatgccgtc gcgcagattt
     7441 atcatttgga caaataacgg tgcgccggaa aaggatgccg tctgaagggg tttcagacgg
     7501 catatttttg tgccgcctga caacatcgtg aaatcccaca ttatatcgat gtaattacaa
     7561 agtattatcc tatatgcgga ttggttttaa gatttgtaaa tttgatttgc atcaaaaatc
     7621 gccgatagat gattcatata atatcaatat taaagagtat cggtatatcg gggatagcta
     7681 tgtcctgttt ttcaatccgg cgtatgtctg cgtttcgggc gcggataacg gcgtttttta
     7741 ccgcctttgt ctttttgacg gcggcactgc ccgcttatgc ggagcgtctg cctgattttc
     7801 tggcgaaaat acagccttcg gaaatttttc cgggtgcgga tcgttacggc aagccggaag
     7861 gcaagcctat ggttgcccgc gtttacaaag gcgatgagca gctcggtttg gtttatatca
     7921 cgaccgatgc ggtcaatacg cgcggttatt cgagcaaacc gatcgatacg ctgatggctt
     7981 tggcaaacga cggcacgata gccggggcga aactggtcga tcatcacgaa ccgattatgc
     8041 tgatcggtat cccgcaatcg cgtgtcgata agttcatcga caaatatatc ggtctgaatt
     8101 ttattaaaaa tccgccgacc ccgagcgtgg cgccgggcga catcatcagc ggtgcgactg
     8161 ttacactgat ggtggttaac gacagcatcc agcgttcgta caaggtcatt gccaaccaat
     8221 accgtctggg ttcggacaag gcccttcaga cggcatccgc ttccgatgtt cgggaagccg
     8281 cgcctgcgtc agaaacccgt ccgcgccgta tggcaaattc cgacaagcag gatatttgtc
     8341 ttgggacgaa cttttcaaac aaaaggccgt cggccatctg catatcacgc tcgatcaaat
     8401 caacaaactg tttgagaaag gcggcaaggc cggcgtggcc gatcacgccg aacagggcga
     8461 tcctgacgat acctttattg atttgtatgt tgccttggtc agccagcctt ccatcggtaa
     8521 aagcctgctg ggtgaggacg gctgggcgca tctgcaaaaa cggctgaaac ccgggcagca
     8581 ggcggttttg gttgccggag agggccgtta ttcttggaaa ggttcgggct atgtgcgcgg
     8641 cggtattttc gaccgtatcg agatgattca gggggagaac agcttccgtt ttaccgatgc
     8701 ccaacacgaa cgcgtcgtcg agctgtctgc cgccgatgcg ccgcgtttta aagaagtttc
     8761 ttggtttacc atccctgaag gcgtagcgtt tgacggtgcg gagccgtggc ggctgtaatt
     8821 gatggttcag cgcgtattga gtgtgaacga taaagccttc gttaccgccg atttggatta
     8881 cgaactgccc caagcttatt acgtcgatga tcctaaagcg ccgcccgtag aaatcagcgc
     8941 gcctgtcgaa gcagtacctg ccgcagcatc cgataccgct tcagacggca ttgccgaaga
     9001 tgcgtcagct gaaaacggtg tatccaacca attatggaaa cagatttgga aagcgaaaca
     9061 ggggcagatt gtcgtcgtcg gcattgcgct gaccatcctg cttttggtat tcctgttcca
     9121 agattggatt gtgcgttacg aaaaatggta cgaccgcttc cgctttgctt tcctaacttt
     9181 tacgctcttc tatatcggct ggtatgcaca ggcgcagctt tcggtcgtca atacgctgac
     9241 gctgttttcc gccatcctta ccgaatttca ttgggaattc ttcctgatgg atccgattgt
     9301 gttcatcctg tggctgttca cggctgcaac catgctgctg tggaacaggg ggacgttttg
     9361 cggttggttg tgtccgttcg gctcattaca ggaattgact aaccgcattg ccaaaaaact
     9421 gggtgtgaaa cagattaccg tgccgcatat gctgcatacc cgtttgaacg tcataaaata
     9481 cctgattttc ttcggttttt ttgcaatttc gctgtatgat ttgggtacgg cggaaaaatt
     9541 tgccgaagtc gaaccgttca aaactgcgat tatcttgaaa ttcatgcgcg attggtggtt
     9601 tgtcgccttt gccgtggcac ttttgattgc ggggctgttt atcgaacgct tcttctgccg
     9661 ttatctgtgt cctttgggtg cgggcatcgc gctgccgggc agattccgcg tgttcgactg
     9721 gctgcgccgc tacaaaatgt gcggcaaccc ctgccaaatc tgtacgcacg aatgccccgt
     9781 tcaggcaatc gcgcccgagg gcgacatcca tccgaacgaa tgtatccagt gcctgcactg
     9841 ccaagtgatg taccaccacg atacgcgctg tccgcaagtt gtggcggcaa ataagaaaaa
     9901 acaaaaacag gctgctgcca aatcagggga attggaaaat gtctctaagc agccgcagga
     9961 acaggtcgtc cgttttgtca aacccgaaac ggcgcaatct gaaaaataag catccggggg
    10021 aagcctgaag ccaaagtgtc aggcaggcag ggtttccccg gaaggataat aaaatttttt
    10081 tcccatcggc ttgccgtccg aaaaccggtt tgccgtattt tatccaagga gtgttgtatg
    10141 tcagacgaaa aattagaaca aaacggcttg agccgtcgtt cgttcttagg tacggccgcc
    10201 gcttccggtg caggtattgc cggtgcgggc ctgttgggtt tggcgggttg ctctaaagac
    10261 ggcgaacagg ctgccgctaa cgcttccggc gcggctcccg tcgccaaggc gcaaggggaa
    10321 tccaaacccg gccaactgtc ttccgaagtc ggtccgggcg aactcgatca atattacggt
    10381 ttcctttccg gcggccagtc cggcgagatg cgcctgatcg gtctgccttc tatgcgcgaa
    10441 ctgatgcgta tccccgtgtt caatatggac agcgcgaccg gttggggacg caccaatgag
    10501 agcttgaaag tcctcaacgg caatattacc gaagaaaccc gtaaattctt aaaagacggc
    10561 ggcctgcgct gctaccccaa cggcgacttg caccacccgc acctgtcgtt taccgaccaa
    10621 acttatgacg gccgctatgc ctatgccaac gacaaggcaa acaaccgcgt ctgccgcgtg
    10681 cgtttggatg tgatgaaggc cgacaaaatc atcgacatcc ctaatgattc aggtattcac
    10741 ggtctgcgtc cgcaacgtta tccgaaaacc ggttatgttt ttgccaacgg cgaacacatt
    10801 acccctgtca gcggtgtagg caaactggat gatgcgaaaa cctggaatgc agtgtacacc
    10861 gccatcgacg gcgaaaccat ggagatcgca tggcaagtat tggttgacgg taacctggac
    10921 aacggcgatg ccgactatca aggcaaatat tcttttgcca cctgctacaa ctccgagcgc
    10981 gcgctgaccg tacaaggtgc gtcttccaac gagcaggact ggtgcgtcgt tttcgacctg
    11041 aaagccatcg aagaaggcat caaagcgggc gacttcaaag aagttaacgg tgtgaaaatg
    11101 ctggacggcc gcgctgaggc caaatccaaa tacacgcgtt atatccctgt gccgaactct
    11161 cctcacggct gtaacgcaag ccctgacggt aaatacatca tgcccaacgg taaactgtct
    11221 ccaaccgtta ccgtattgga tgtcagcaaa ctggacgatt tgttcgccgg caaaatcaaa
    11281 gagcgcgatg tggtcgtagc cgaaccgcaa ctgggtctcg gcccgttaca cactgcattc
    11341 gacggtcgcg gcaatgctta tacgacattg tttatcgaca gccaaatggt gaaatggaat
    11401 attgacgatg cgatcaaagc ctacaaaggc gataaaatcg atccgatcaa acaaaaactc
    11461 gacgttcact atcaaccggg tcacaaccat acgaccatgg gcgaaaccaa agaagccgac
    11521 ggtcaatggc tggtgtcctt gaacaaattc tccaaagacc gcttcttgaa tgccggttct
    11581 ttgaaacccg agtgcgacca attgatcgac atctccggcg acgaaatgcg tctggtacac
    11641 gacaacccga cttttgccga accgcacgac ttgtgtttgg ttgccgcgtc caaactgaac
    11701 ccgggcaaaa cttgggaccg caaagacccg tggttttagc aggatgcttt ggaacaggcg
    11761 aaaaaagacg gtgtcgagct ggaaaaagcc gccaaagccg tacgcgaagg caacaaagta
    11821 cgcgtgtata tgacggccgt cgcgcctgct tacagtatcc cgcagtttga agtgaaccaa
    11881 ggcgacgaag taaccgttta cgttaccaac gtcgaaacca tcgaggacct gacccacggc
    11941 ttcactttgg aaggctgcgg catcgctatg gaaatcggcc cgcaagccac ttcttccgta
    12001 accttcaaag ccgtccgccc gggtgtgcac tggtactact gccaatggtt ctgccacgca
    12061 ttgcatatgg aaatgtccgg tcagatgatt gttcatccca aataatgcgg cccgacggct
    12121 gaatatggag aatgccgtct gaaggcaata ggtcttcaga cggcatttat agccaaaccg
    12181 cttgccgtac cgtttcaggc ggtttcgcta taaaaacagg cacatttgaa tgatgcttta
    12241 ttttgttcgg ccataccgaa cgaaacagag ggacactatg cacacatccg cgctccgtat
    12301 atggctgaag gccgtgctga tacttgccgc cggcagcatt tttcagacgg catcggcagc
    12361 cgtcgtccac gtttccccgc aagacaacct tgccgaaatt tttgcccgcg cccgggcggg
    12421 cgatacgatc aaactggctt ccggcgtata tcaaaccaaa ctttatatcg acaaaccgat
    12481 tacgattgaa gggcctgccg accgttccgc aaccatcgaa ggggacaaga gcgggcgtac
    12541 catagccgta cacgcgccgg acgtaacgct ccgcaacctg accgttaccc gttccggtat
    12601 gagcctgcca gcaatggatg ccggtattta tctcgaaaaa gccgccccgc gcgccctgat
    12661 tgaacacaac aatatttttg ataattcgtt cggcgtatac cttcatggtt ctgccgatgc
    12721 gatggtgcgc gagaataaaa tcgtcggcga tgcgaccttg cgcgtgaatg agcgcggcaa
    12781 tggcgttacc gtttggaacg cgcccggcgc gcaggtcgtc ggcaacgaca tttccaaagg
    12841 gcgggacggc attttttcca ataccagcac gcacaacacc tataaaaaca accgcttcag
    12901 cgacctgcgt ttcgccgtcc actatatgta caccaacgac agcgaagtca gcggcaatat
    12961 ttccgtgggc aacaatatgg gctatgtgct gatgttttcc gaacggctca aagtgttcga
    13021 caatatcgcc gtcggcagcc gcgattaggg catcatgctc aactatgtca actattccga
    13081 tattcacgac aatattatca acaaagcggg caagtgcgtt tttgcctaca atgccaacta
    13141 cgataaactg tccgccaatc attttgaaaa ctgccaaatc ggcatgcact ttaccgccgc
    13201 catcgaaggc gcgtccctgc acgacaattc ctttatcaac aacggaagcc aggtcaaata
    13261 tgtcagtacg cgctttctcg actggagcga gggcggacac ggcaactact ggagcgacaa
    13321 cagcccgttc gatttgaacg gcgacggctt cggagacagc gcgtaccgtc ccgacggcat
    13381 catcgaccaa atcatctggc gcgcgcccgt atcgcgcctc ttgatgaaca gtcccgcaat
    13441 cagcatcgtc aaatgggcgc aggcgcagtt tcccgccgtt ctgcccggcg gcgtggtgga
    13501 cagcaaaccg ctgatgaagc cttatgcccc caaaattcaa acccgttatc aggcgatgaa
    13561 ggacgagttg ctcaaagaag ccgaaacgcg gcagtcggaa cggggcaggg cggaaaacgg
    13621 ttctttgaac tagtctgctt cagacggcac ccggattcaa atgccgtctg aaaacacaaa
    13681 aggaacaacc atgaccacac atcatgtcga attgaggaag gtaaccaaac ggttcggggc
    13741 gcaaaaagcc gtcagccaag tcgatttggt tttgaaggca ggagaaagcg tcgggcttgc
    13801 cggacacaac ggcgcgggca agtccaccat catgaagctg atactcgggc tgattacccc
    13861 gaccgaaggc gaagtgatgc ttttgggcga acgtaccggc agcaaagcgg gggcgcggct
    13921 tcgcagccaa atcggctacc tgcccgaaac cgttgcgctg cacccttcgc tgaccggcat
    13981 cgaaacgctg gatttttatg ccaaacttaa aaaacagccg ctcacgcaga accgggggct
    14041 gcttgagcgc gtcggcattt cccagaccgc acaccgccgc gtcggcactt attctaaagg
    14101 gatgcgccaa cgccttgcct tggcacaagc cctgctgggc gagcccaaag tcctgctgtt
    14161 tgacgaaccg acaaccggcc ccgaccctgc atcacgacaa atgttttacg aagtcgtgcg
    14221 cgaactcaac gggcgcggcg cgaccatatt gctcagtacc cacgcccttg ccgagttgga
    14281 cggacacgcc gaccgcattg tcgtgatgaa aaacggcgtt aaggttgccg acggcagtat
    14341 ggacgaattg cacgtccaaa gcgggctgcc gctgaccgtc aatatccgcc tcaatgcgcc
    14401 gcgcaccctc agcagccgct ggcagccgct ttcagacggc atttcctacc gggcgcaatg
    14461 tcaggccgaa gaacgtatgg aactgttggg cgaattgggc agcctgtccg accttgccta
    14521 tttcgacatc cacacgccga cgcttgacga aatgtacgca cggttcttga aaagggagga
    14581 cgtatgaacc ccgtttggat tattaccggc aaagaagccc gcgacagcct gcgtaaccgc
    14641 tgggtgcttg ccgccgtcct cctgcttgcc gcactcgccc tttctttggg tttcctcggc
    14701 agctcgccca ccggttcggt caaggtcgat ccgctgaccg ttaccgtcgt cagcctgtcg
    14761 agcctgtcta ttttcctgat tccgctgatt gcgatgctgc tttcttatga cgcactgatc
    14821 ggcgaaatcg aacgcggtac gatggcgttg ctgttgagtt atcccatttg gcgcaaccaa
    14881 atccttgccg gcaagtttgt cggacacctc atcatcctcg cccttgccac cacggcaggt
    14941 tacggattgg caggcattac gctgcaactt gccaacggcg gtttcgacat cgccgtttgg
    15001 aaaccctttg cgctgttgat tgccgccagc gtgattttag gtgcggcatt tctgtctatg
    15061 ggctacttga ttagtgcaaa ggtcaaagag cgggggacgg cggccggtat ttccatcggc
    15121 gtatggttgt ttttcgtcgt catcttcgat atggcgcttt tgggtattct ggttgccgac
    15181 tcgaaacagg tcatcaccgc gcccgtcgtt gaaacggtgc tgctgttcaa tcccaccgac
    15241 atttaccgcc tgctcaacct gaccggttac gaaaatacgg ctatgtatgc gggtatggcg
    15301 ggtttgagcg gacaaatcgg cctgaccgtc cccgttttgc tgaccgcgca ggttttatgg
    15361 gttatcattc cgcttgtttt ggcagccgga atttttagaa agcgacgaat atgaagaaaa
    15421 ccctgttggc aattgttgcc gttttcgcct taagtgcctg ccggcaggcg gaagaggcac
    15481 cgccgccttt accccggcag attagcgacc gttcggtcgg acactattgc agtatgaacc
    15541 tgaccgaaca caacggcccc aaagcccaga tttttttgaa cggcaaaccc gatcagcccg
    15601 tttggttctc caccgtcaag cagatgttcg gctataccaa gctgcccgaa gagcccaaag
    15661 gcatccgcgt gatttacgtt accgatatgg gcaatgttac cgattggacg aatcctaatg
    15721 ccgacacgga gtggatagat gcgaaaaaag ccttttacgt catcgacagc ggctttatcg
    15781 gcggtatggg cgcggaagac gcgctgccgt tcggcaacaa ggagcaggct gaaaaatttg
    15841 caaaggataa aggcggcaag gtcgtcggtt ttgacgatat gcccgatgct tacattttca
    15901 agtaaatcca aacagaaatg ccgtctgaac gttgaagcca aggttcagac ggcatttttt
    15961 gtcataggtt caaacggggc aggttcgaac aactgcccca ttccttattc ctgaccgggc
    16021 gtaacggcgg aatgaggttt acatcccgcc gtaattcggt ccgcttgcgc cttcggggca
    16081 aatccaagtg atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt
    16141 tgccgcgttg atttgcaggc gcggactgcc gttttcttcg atgatttcat atacgccggc
    16201 cggacaatag cgcgtttcgg gagaggcgta ttctttatag ttcacgtcta tcatcgtttg
    16261 cggattgttc agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa
    16321 gacgctgctc aagcggtcaa aggtcaacac accgtcgggt ttcggataat caatcggctt
    16381 gcacacggcg gcttttttga gctgctcgtt gtctttgccg tgatgtttca aggtccacgg
    16441 ggctttgcct ctgaaaatca tctggtcgat gccggtataa agcgagccga ggtaaacgcc
    16501 ccatttgaat gacggacgga cattgcgcgc ggcataaagc tcttgataca gccagctttg
    16561 ttcaaacagt ttttgatagt ttcccgcttc tttgccgctg tcgaagcttt ccacttcttc
    16621 aaagttttcc aacaagggga acacggcttc ggcggcgagc atggcggatt tcatcgcggt
    16681 atgaatgcct ttgatgcgcg gcatattgag gaaccccgcc gcatcgccga ttaaaacgcc
    16741 ccccttgaac gagagcttcg gcaggctttg caaaccgcct tcaatcagcg aacgcgcgcc
    16801 gtaagcaatg cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg
    16861 ttggaactct tcaaacggcg acagatacgg gttttgatag tccaaaccga ccacgaagcc
    16921 gacggcgact ttgttgtcgt cgaaatggta aataaacgca ccgccgtagg ttttgctgtc
    16981 cagcggccag cctgcgctat gcaccaccaa accgggctga tgctgttcag acggcacttc
    17041 ccaaacttct ttgatgccca agccgtaagt ttgcggctgg ctgttttggt cgagttggaa
    17101 acgttcgatg atttgcttgg aaagcgaacc tcggcaacct tcggcaaaca gggtttgccg
    17161 cgcccaaagc tccatgccgg gctggaaact gtcggtcggc ccgccgtctt tgcctacacc
    17221 catattgccg gtcgcaatac ctttgaccga gccgtcttcg tgatacagca cttcggcggc
    17281 ggcaaagccc ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac
    17341 gacttcgcct aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taataggcag
    17401 attgaacgct tttttctccg tcaggaacaa cactttgtcc tgagttaccg tgcgcgtcag
    17461 cggcgcgcct ttttctttcc agtcgggaat cagctcattc agcgcaatcg gatcgataac
    17521 cgcgcccgcc agcgaatgcg cccccgcctc cgaacccttc tccgccacgc aaacgctgat
    17581 ttcgcgcccg ttctgttcgg caagctgctt gagtttgatg gcggcggaca accccgacgg
    17641 gcctgcgccg acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat
    17701 ggcggttcct gtgtatttat tattgaattg caaatccgtg attatacaac gggaacatat
    17761 agttaccaaa tacaacaaag gtcgtctgaa accatgtttt cggttttcag acgacctttg
    17821 tcgaaatttc aataagcacg ccaccatttt acctgtccga ccgcaaactc cgtctgacgt
    17881 ttcggactgc gtgtgaaaaa cgccttatcc ccaccggcat ccctcccttt cggcacgacc
    17941 gccaaaat
//
LOCUS       DAXFHF010000047        17748 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXFHF010000047 DAXFHF010000000
VERSION     DAXFHF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17748
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.047"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(54..608)
                     /locus_tag="ACFIRM_001445"
     CDS             complement(54..608)
                     /locus_tag="ACFIRM_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029044.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFE
                     LLIDEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMK
                     EIFDEILRIENLDRKNLPSNIMVK"
     gene            complement(695..1744)
                     /locus_tag="ACFIRM_001446"
     CDS             complement(695..1744)
                     /locus_tag="ACFIRM_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC3029045.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEEFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
     gene            complement(1936..3453)
                     /locus_tag="ACFIRM_001447"
     CDS             complement(1936..3453)
                     /locus_tag="ACFIRM_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029046.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGHS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALIAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVELARNNLRKFYSKR"
     gene            complement(3453..4196)
                     /locus_tag="ACFIRM_001448"
     CDS             complement(3453..4196)
                     /locus_tag="ACFIRM_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029047.1"
                     /translation="MDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            complement(4205..4810)
                     /locus_tag="ACFIRM_001449"
     CDS             complement(4205..4810)
                     /locus_tag="ACFIRM_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029048.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISKAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            complement(4800..6320)
                     /locus_tag="ACFIRM_001450"
     CDS             complement(4800..6320)
                     /locus_tag="ACFIRM_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC3029049.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAVKQDVNLHQKEVSENNTESKDSDMEFVPAKVNGTKADAEDEP
                     KEAEPITLEQTSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAG
                     NAIYAIDVLHEEAGNVFTFLWKLSGIDTFMFDVGDSSNSLLTLKSDSKLRIAYEDPDK
                     YDEYTNCGIGNKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            complement(6317..7204)
                     /locus_tag="ACFIRM_001451"
     CDS             complement(6317..7204)
                     /locus_tag="ACFIRM_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3029050.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            complement(7445..7627)
                     /locus_tag="ACFIRM_001452"
     CDS             complement(7445..7627)
                     /locus_tag="ACFIRM_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029051.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            7627..7962
                     /locus_tag="ACFIRM_001453"
     CDS             7627..7962
                     /locus_tag="ACFIRM_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029052.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKVVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(7999..8658)
                     /gene="ung"
                     /locus_tag="ACFIRM_001454"
     CDS             complement(7999..8658)
                     /gene="ung"
                     /locus_tag="ACFIRM_001454"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC3029053.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            8739..9146
                     /locus_tag="ACFIRM_001455"
     CDS             8739..9146
                     /locus_tag="ACFIRM_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC3029054.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            9161..10108
                     /locus_tag="ACFIRM_001456"
     CDS             9161..10108
                     /locus_tag="ACFIRM_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC3029055.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(10215..11285)
                     /locus_tag="ACFIRM_001457"
     CDS             complement(10215..11285)
                     /locus_tag="ACFIRM_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC3029056.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            11470..11745
                     /locus_tag="ACFIRM_001458"
     CDS             11470..11745
                     /locus_tag="ACFIRM_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC3029057.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            11747..12370
                     /gene="lipB"
                     /locus_tag="ACFIRM_001459"
     CDS             11747..12370
                     /gene="lipB"
                     /locus_tag="ACFIRM_001459"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC3029058.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            12363..13346
                     /gene="lipA"
                     /locus_tag="ACFIRM_001460"
     CDS             12363..13346
                     /gene="lipA"
                     /locus_tag="ACFIRM_001460"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC3029059.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            13821..14285
                     /gene="bfr"
                     /locus_tag="ACFIRM_001461"
     CDS             13821..14285
                     /gene="bfr"
                     /locus_tag="ACFIRM_001461"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3029060.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            14313..14786
                     /gene="bfr"
                     /locus_tag="ACFIRM_001462"
     CDS             14313..14786
                     /gene="bfr"
                     /locus_tag="ACFIRM_001462"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3029061.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            15114..15434
                     /locus_tag="ACFIRM_001463"
     CDS             15114..15434
                     /locus_tag="ACFIRM_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3029062.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFCVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            15483..>17748
                     /gene="glnD"
                     /locus_tag="ACFIRM_001464"
     CDS             15483..>17748
                     /gene="glnD"
                     /locus_tag="ACFIRM_001464"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC3029063.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPVRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSC"
BASE COUNT         5005 a         4576 c         3777 g         4390 t
ORIGIN      
        1 aaaaaaacca tcacaaaagt gatggcaaat gatttttaag gctattaaat atcttactta
       61 accatgatat tactaggaag atttttgcga tccaaatttt caattcttaa aatttcatca
      121 aaaatttctt tcatcgaaaa caattctgcc aagtctccgg caatctgcct acctcggcaa
      181 aaatactgtc tgcaatcaaa ataaaccttc atatgtaata accgttcatc aaggctggca
      241 ttcgtatcat ccaacgccgt actaatatcg gcccatttaa aaaacttctt tctcgacata
      301 cggccactgg aagaaacaac atttttgaaa aaaatcatat tcggaatata gtattttttt
      361 aaatccaaac cagccaaact tttattactc aatttcagat gttctcttac tataggatat
      421 atgagattaa ggccctgaca tgcttcatga aacgactgag cttcatcaat taatagctcg
      481 aaatcaacca atttcaattt aatttctttt tctaaatcct caatccccct tacccttaaa
      541 agccttttgt attcccaaaa ttttaagtat tttttcatac ctgatatttg agtattcata
      601 gtattcatat cacatcataa aaaaatccat acaaacaagg gccaatcaat accatcttaa
      661 aactcgattg taaccccacg ttccaatttc acaactagcc cgataatcca ttgtcacgaa
      721 caatatcaac caacttattg agatcctcct gattcatttt tctctggata taaaccaaag
      781 agggttgtga ttcatacacc caaacagaca atggaattct aggcagaatc tcttgctcaa
      841 cagcagattt caacttcgac aactgctctg ggtcaagcag actgtcacgt cgaatgctct
      901 tgatagccct ggtctccatt accgcaatat ataaatcatt cagtagcttt ctcatcatcc
      961 gaccgccatt atacaacgtg tcattacatt gtttattagc catcatgcct ttgatattaa
     1021 gtgtcttaac ggcacataca aaactatcgt aatcccgcaa caatttaacc agtttgaaac
     1081 cataagcccg caatacattc aatctgatat gaacagggtt attgttctga atcaaggtta
     1141 tcttcatacc tgcagcggcc aaactatcaa tctgagcatg aacctgtttt ttcacagtct
     1201 caaaaaattc ctcaatttcg gacatctgtt tttcaatctg caataaccga ctttgggcat
     1261 aaggattatc ggacatagcc ataccaaaca actcgttact gagtgtcaga ccatatctga
     1321 ttcccggcca acgtgggccg ttcttcttgt tgttattgcc ctcccacata cggatacctt
     1381 gcttggtatg cataaaaaaa ccatcatcac cggaagaggt caggctgcca atatttttaa
     1441 accggtcgtt ctcagaagaa agaatttcat actcccccca aactaagtga tgctttcgac
     1501 ctgtctcctt ttgtagatgt tgcttgagca gcaattcatg tgctttaaac tcttcccact
     1561 gttggaactg ttcgtcatcc tcagtcaact tttgattgac cttatccccc aaagccaaag
     1621 cagcacgagc catatcgtaa ccgtcactga aaggtgactg ttttgttgtg actttgagat
     1681 caaattgctg tgcaacatgg atcgcaccag gtttgatgat atatctttgt tttgcaaatt
     1741 ccatttttaa atccttataa gttattaaaa agagcttcat aaagcactgt tgttgcatat
     1801 cagaggctaa ttagtcatgt acaccatcat atctgtcctt tgccgatgac tgtaacgccc
     1861 cgttacatcc gcttcgaagt aatagattta ttcgtctttc aaaaattcat caaaaatatt
     1921 tttttaaatt aaattttatc ttttggagta gaactttctt aaattattgc gggcaagctc
     1981 gacatccatg gcatctaatg agtcctgttt ttggttattc tgcttttcaa gataagcctg
     2041 taatttctga tcctgctcac gctggaatct aacctgtggg gcgataagag cagtaaagtt
     2101 accttgcgaa gcgcagcggc ataggttaat caaaagacca agggggctag taacttcttt
     2161 tttctgtccc tggatctcat ccaagatttc ctgcctcaaa gcccaggaac tttttgagag
     2221 atgttttgta aaaatcaaat ccgcctgttg acgcatttgc acattaaaaa gttttggata
     2281 aatcaaatca acatcatcaa ccactaccac atgatcttgt tcggaagaac gtggtttgtt
     2341 gggttcaaca gtcatgtagt tgtagttata aacccctttg taatttatat aataaataat
     2401 ctgccgattc ggcaattctg aacttcccca atcgacactt atgaatttcc cactatgacc
     2461 tttcttaaaa ttccaattcg acacatccga atattccctt tcgacacttc cggatgtcat
     2521 acttgggaat aggggggcat caccctcgaa accgctctga aaccctttat ccaatccatt
     2581 tggccctttc cgccaatcac atttggccct ttccgccaaa ctgagaattt cgcgtttttt
     2641 ggagttatat ggactaaagc cgtcgtcctg tttgccagct agaagttttt tgatggatat
     2701 aacaatatcg tgaccattta ccagggtaaa aatacggcgg ctggccaaga aatttctctc
     2761 ataaatgaga tttaaattct tcagcctatt gcgagctgtc aattgagatt tataagagag
     2821 agaggttctt tcctcccaat cagcagcagt caaagtagca aaggcatgac gttgtaaatg
     2881 tggattttga gaaaccaaac caaccgcatc atttaacaca cacgacaata atacagccga
     2941 gttgatacag cctgtaatag aaaccaggtc tcggtgataa gcaatccttt tagaaaagtg
     3001 ttttctaaaa tacagggaac ttgtgttctg taaatcagcc agcgtaagtg caagttgatc
     3061 gtcagtaccg ctcgatttag cgatcaactc agctatggca tccaagttca ctttgattgc
     3121 taaagcagcc cccacacctt tacggcatgt ttgaataagg tttgtctcaa gaagcttgtt
     3181 tttacaagtg cgctgctctc tcactgtcaa accagtttca gcttccatct gagcaataga
     3241 cttgaaaatc caaccatccc gttcggcaac ctctttgctt actcttgtcc aatagattaa
     3301 ctgagaaagc attgcagcag tcttcagaga gcctgtaata tcaattaaac atcgatagat
     3361 ggcaactctt cgaccacctt gcaatgcttg ccagcattgt gtcagttcag agttaaatac
     3421 tttgggaggg ggtaatttgg tggcggtaac catcacacac atccgttcaa atattcagaa
     3481 gtaagatcag caagcatttt caagctgtat ctacaacgtc gcgctgctgg cataccatca
     3541 acacagctct caatcaaacc aaaagaggtt aattcggcaa tagctctttc ctgttgccgt
     3601 cttgacaaca ttgttgaata aaaaacgtgc ttttcagctg catgagtcca aggagaccag
     3661 tcaccacgct tactcaaatc ttgatattca gcttcacttt gaagaaacga caaatacaaa
     3721 atggatacaa aagcaagacc tctatcggct aatgtcttca ggtgaagttt attaatcgaa
     3781 atagggggcg aaaccatgtc aagtacatct gatttaccat gtttacttac caaatcctca
     3841 aaaagagcat cattcaatgt gaattgtgat tcggttggcg ctacggttct ccgattaagc
     3901 aaaatacctt tctgcagaag gctattgcga atattccgcc actgctttgc ggtaatacct
     3961 gcttcattaa acaacagatc attattcagg cttacccatt cgccaggcag acatttctcg
     4021 acaaaccaac tcaaaaaata tccctctgaa acagaaccga ccgcctgagc aaactgacgc
     4081 acatatgtgg ccggttcctc ccacaaggca accaagctac ggataaagtt atcggttgac
     4141 atgttcttag atggtacaaa accagtctca taatcatagg aagtagagaa atccatatac
     4201 atactcatac tccaaagaac tttacatcgt taattattga atgtaattgg gcaaggctat
     4261 aatcggggaa ttcatcaatc aatagctggt atctaataaa caaatctggc tctttttccg
     4321 acttactcca tgccttcaat accctgacgc gaacatcaat agatggcatc acaatgcgcc
     4381 ctttaacgac aggaatattc atttctatac gcaatttttt aatttccgcc ttagaaatta
     4441 atgaacatag attcataatc atcagattgc tggccttatg ttctaacatc catttaagac
     4501 gctgcatctg ttccataaac ctttgttcgt aatgttccat ttctgaaatt ttgcttttta
     4561 aaaccttcag atcaatggag taggtcacaa catcaggaca catggatgct aatttctcaa
     4621 tccgcttcgg gttcattcgt ttgagcgtgt tcaaaacatc ctcatgttca cgagcaacca
     4681 aagcgttata agaagattca cgcggcgtat ttggtgcgga tgccaacctt gtaagtgcaa
     4741 gattggtata aaccggatcc gtattaaaaa gtctataaat gggattacta attaaaggct
     4801 tactcctcac tcttagctcc cgtttttctc aagttgttgt actgtgtgct cagagataat
     4861 atttctacga tattggcgtg aaattcatgg ctggtattag tttgccaatt cagtaaagag
     4921 gcaataatgt tctctttatt accaattccg cagttcgtat attcgtcgta cttatcgggg
     4981 tcctcgtatg caatacgaag cttagaatca cttttaagtg tcagcaggga attggaacta
     5041 tcaccaacat caaacataaa ggtgtcgatt ccactaagct tccataagaa tgtgaaaaca
     5101 tttcccgctt cttcatgcaa aacatcaata gcatagatgg catttccggc acttgatgct
     5161 gaaatatgct caaaagccgg atattcgaca taccagccgt atggaaagtc cttatgaatc
     5221 ttaaagcaat cggacagact cactttggct aacagtttac gaacagcaga aaacagtttt
     5281 ttgctggttt gctccaaagt aatcggttcg gcttctttcg gctcatcctc tgcatcagct
     5341 ttagtaccat ttactttggc tggcacaaat tccatatccg aatcctttga ctcagtgttg
     5401 ttttccgata cctctttttg atgcaaattg acatcttgtt tgacggctgt ttcaattttg
     5461 tctttatcag aagaattttc tgtttcagaa aaggcgtcag agccatattc aaatttaaat
     5521 ctgtcgagca cgaactgata aagctcatgc acgtctaact ctgttgcaga atattggtta
     5581 tgccactgct ccagtacatc cgaccacaaa tattggaaag aatcgtccaa ctgaattgat
     5641 ttagaaattg agaatagggt agagtgaagt tttttaattt cttctacttt tgcatgggaa
     5701 agtttgaatg cgtattcttg cagctcttgt aaattatcgg aggcaaaaaa gaataatcct
     5761 aaagtcttat gagaaaccgt aaggccttta tcagaaaaca tcacttccag ttcgcgcaaa
     5821 gaaagtttcc ctgagtcaga ttcatcttgg aaaatatcgc ggatttcggc atatttacag
     5881 gccttatccc aaaaacacat ttcagcacgg agttcatttt caacaagatg cgcaacctgc
     5941 aatgtagttt cgtcagagta ggggatgtaa atacaaggca tcatacggaa gcggttatca
     6001 ccagtttctt ccaagagttc gcacaagatt ttgagacggg tattaccacc ctgtgccagt
     6061 acatatttac tttcaccagg tctctgggta acgtgtacag gttgttgaat acctgaagaa
     6121 cggatggact ctttgatggc cgcgtagctg gaaacatcat gtaaacggcg agggtttttg
     6181 tcaaagaaat cgatttcgct gattggcaac gacattaaca tgccccgaga aggagtatcc
     6241 tcttggccaa aactcatatt tgtctgtgca ggggtaccga tgtgcaggct caaggttgct
     6301 ttattttggt ccaaattcat gattgcacct ccttttgcag caggctggcc atgtcattga
     6361 aacagcttcc acgcaggttc ttctgttcga taccagggaa aatctcataa acaatacggt
     6421 gcatgatttc ataagcaggc agcatctttc caagctgttt acgctcatga caatgaaccg
     6481 ggactcttaa agttgtagct tccttatatg ctttggcagc aggaactgct acgcttaaca
     6541 atttacgtct gtcatctaga gtattgctaa agaaatcttt gatagaagat gcgatgaatt
     6601 tcgcatcatt ggtacgatct tgtgcgtaga tgatggctcg taattgaggc atcgggatac
     6661 gcattgcttc gcctttttgc agacgcttta gaagttcttg cgtaccggta aggaactcac
     6721 gggcactcaa aacttcgggc ataattgggc aaagcaggca tgtagcagca aatgctgcgg
     6781 tatctaagag aggaccaact gcaccctgag tatcgattaa aaccacatcg tagttattct
     6841 gtatgtattg ggaatgcagt ttgcttttga gcaagaaggc acggtcggga cgagaggtta
     6901 cttcagtctc gacaaaagca gaaatgttat tggaaataac aatgtctaaa ttcggaaaaa
     6961 cggtattgga aattgtcgag cggatgattt cttcggtatt ttcaccgaga agtaattcaa
     7021 caataccatt tggagcgcgg tggcttattg gatagtattt tgacaatgat ggctgaacat
     7081 cgcagtcaat cattaagaca cgcagtccga tatcagcgag aacagcagag acattagcaa
     7141 tgagtgttgt tttacctacg ccaccttttg tagcggctgc agacaatatt acgggtgcgg
     7201 acataatatt tccttatatt taattgattt ataaagagaa aaatatccgc aatcgaacag
     7261 gtttaaaata tgaaatttac atatttattc tagttcgcat aatgtatatt atgttaaatt
     7321 tataatggat tgaatagata tttttacaat ctcattcaaa actcagacac ttaaaaaatt
     7381 taatcagaac aaataaaaaa gcaaacaaaa atatccatct ttcaaactca tattttgcaa
     7441 taacctagat aagcaagaac aggggcagaa cggattcgcc tttgatgtgg tggtaggcaa
     7501 ccattccaat atgtcccagc gacaaaaaca gcaatgaaaa tgccagcgtg ccgtgaattc
     7561 ctccgccatt gccgatttga cgggcaatgc ctgcggcgga acagcgagca tcagggcata
     7621 cgcccaatgc ccaatttggt gacggcggtg ttggcggggc ggtttttacg ttggcggatt
     7681 ccctacccta cgcaccaaac agacaatatc agcagcagga ttctgactgc acggatatgt
     7741 tggtatgaat cgtccgccca agcggaaaag gttgtcgcgc ccaaaacagt ggcaagaata
     7801 aattgcttga atttcatgat gttgcttctt ttgcacaaag cgttcggaat agtgaagccc
     7861 gcagcctatg gctttgcggc agtcgcagct ttacaagcgg tattgcagga aaccttaaaa
     7921 cacgatcatt gcatcaagag ctgttcgaat tccgccaaat gaacctgaca cgcaaacttt
     7981 gacgcttgaa tacggcattc atagcttcca gtttatcggt tcgataccgt gttgactcaa
     8041 atagctgttt gcctgtgaaa aatggcggca gccaaaaaaa ccgcgatatg ccgacagagg
     8101 agacggatga ggcgcggtca gtatcaaatg attttgactg tctatcagcc tccctttttg
     8161 ttgtgcatac ccaccccaca acatgaagac aaggtgcttg cggtgtgtcg caagctgcct
     8221 gatgacggta tcggtaaagc gttcccagcc taaaagggcg tgcgaatgcg cctgtcctgc
     8281 acgcaccgtc aaaaccgtgt tcagaagcaa tatgccctgc tccgcccacg ctgtcaggca
     8341 gccgtgcgcg ggaatggaaa agccttcgat gtcggtttcc aattctttgt aaatattgag
     8401 taaagacggc ggtatgcgga caccctgccg gacggaaaat gccaaaccgt gcgcctgccc
     8461 taccccgtga tacggatctt gtccgagaat gacgactttg acccggtcga acgctgtcag
     8521 ccggaatgcg ttgaacacat cggcctccgg cggatagata atttgccccg acaaacgttc
     8581 ctgcctgacc gcatttaaaa tttcctgaaa atacggctgc tgtttttcgc cgccgagtgc
     8641 atcgtgccaa gtgtccatat ctgccctatc ctttcaaatt cggctatact gctgatattg
     8701 cgttattttg tcaaacgacg cgctttgtgg ggaaatgtat gactgtatgg tttgttgccg
     8761 ctgttgccgt cttaatcatc gaattattga cgggaacggt ttatcttttg gttgtcagcg
     8821 cggctttggc gggttcgggc attgcctacg ggctgactgg cagcacgcct gccgccgtct
     8881 tgaccgccgc actgctttcc gcgctgggca tttggttcgt acatgccaaa accgccgtgg
     8941 gaaaagttga aacggattca tatcaggatt tggataccgg aaaatatgcc gaaatcctcc
     9001 gatacacagg cggcaaccgt tacgaagttt tttatcgcgg tacgcactgg caggcgcaaa
     9061 atacggggca ggaagtgttt gaaccgggaa cgcgcgccct catcgtccgc aaagaaggta
     9121 accttcttat catcgcaaac ccttaacact cggaggaatt atggaatttt tcattatctt
     9181 gttggcagcc gtcgccgttt tcggcttcaa atcctttgtc gtcatccccc agcaggaagt
     9241 ccacgttgtc gaaaggctcg ggcgtttcca tcgcgccctg acggccggtt tgaatatttt
     9301 gattcccttt atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt
     9361 acccagccag gtctgcatca cgcgcgataa tacgcaattg actgttgacg gcatcatcta
     9421 tttccaagta accgatccca aactcgcctc atacggttcg agcaactaca ttatggcaat
     9481 tacccagctt gcccaaacga cgctgcgttc cgttatcggg cgtatggagt tggacaaaac
     9541 gtttgaagaa cgcgacgaaa tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg
     9601 ggcttggggt gtgaaagtcc tccgttacga aatcaaggat ttggttccgc cgcaagaaat
     9661 ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc gtattgccga
     9721 atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt ggtcagcgtg aagccgaaat
     9781 ccaacaatcc gaaggcgagg ctcaggctgc ggtcaatgcg tccaatgccg agaaaatcgc
     9841 ccgcatcaac cgcgccaaag gcgaagcgga atccctgcgc cttgttgccg aagccaatgc
     9901 cgaagccatc cgtcaaattg ccgccgccct tcaaacccaa ggcggggcgg atgcggtcaa
     9961 tctgaagatt gcggaacaat acgtagccgc gttcaacaat cttgccaaag aaagcaatac
    10021 gctgattatg cccgccaatg ttgccgacat cggcagcctg atttctgccg gcatgaaaat
    10081 tatcgacagc agcaaaaccg ccaaataagc cgtgatgaaa ataccgtctg aagctctgtt
    10141 ttcgggtttc agacggtatt tttatttaaa cccgaacagg gttgtattcc gcttcaaata
    10201 tgtttcggaa ccgtctacct gccccggtaa aaactgccgg caaaatattt ctgcgcgccc
    10261 tcgcgaagca agaccaaggt tacggcggcc aaaggcaatc cggccaacat tccgacaaag
    10321 cccatcagct ctccgaacgc catcagcgaa aagataaccc aaaacggcga caggccgata
    10381 cggtctccta caattttcgg cgtaatgaaa aaactttcga gaaactgacc gacggcaaaa
    10441 accgcccaaa cagccaagat tccgttccac gaaccgaact ggagcaaggc tgcaacagtg
    10501 gcaagcagca atcccgtaaa cgcacccaaa taggggacaa acaccaaaat accggcaacc
    10561 ataccgatgg caaatcccga atccagtccg actagcatca atcccaaacc gtaaaccaag
    10621 cccataatca gcatcaccag aagctgaccg cgcaaaaatt cgcccaatac ctcgttcaaa
    10681 ttacccgtaa tgcgcgtata agcaccggca aaacgcctcg gaaccagttt ggcgatgccg
    10741 cacgaccacc gctgccaatc cagcaggaaa tagtaaagca gcaagggcgg cagcagcagg
    10801 ttgccgatac tgctgacaat attgccgccc tgtttcatca aaacgggaaa ccacgcctta
    10861 agcgcgttgc tcaactcgcc cgtatgcgcc tgaaaccacg caataataga tgcctgatcg
    10921 atttccacat atccgccgat tgtatttttc aaccacggca gcagcgtgtt ctgcataaaa
    10981 ccgattaatt ggggcaggcg agatgccaaa ttattgaact gcccgaccag catagggaca
    11041 ataatcaaca ataatgccaa caacaaaatc aaggaaaaca ccatcacaga catcgaagcg
    11101 gatgcacggt tcaaaccctt tttctgcaac cattcgacca aagggtccaa cacatacgcc
    11161 agcaccgccg caaccgcaaa cggagtcaaa gtatcgccga gcgcgtaaac cagccagacc
    11221 aaggcggcaa acgccgcgcc ggcacccatc cacggcttga tgccccgtcc ttttctccga
    11281 tacataaaat ccacccttcc gaaaataata ttgcgcgcca gtatagcaga accgccgcgc
    11341 cgtcaaaaaa gcctccgcct tcccgatagc ctgttatcgt ggtttaggct aaaatactgc
    11401 acatccgatg ccctctgaaa ggcaaacccg ccttcagacg gcataccgta cacgataagg
    11461 cagcacatca tgaccgaaca aaaaaacaaa gcatccctca tcgaatttcc ctgcgccttc
    11521 ccattgaaag tgatgggcgc ggtgcatccc gagttcgagc aggcggtatt ggacaccgtc
    11581 cgccttcacg cccccgacac gcaggcgcac cacatcacca cgcgtccgag cagcaaaggc
    11641 aactataccg gtgccaccgt tcaggtaaag gttgaaaacc aagaacaatt ggacaacatc
    11701 taccgcgcgc tgacttcgca cgaactggtc aaagtggtac tttgagatga aaatcataca
    11761 caaaggtttg gtcgaatatc tgccgacttt tgaagcgatg aaaaccttta atgccggacg
    11821 gaatgcagac accgaagacg aattgtgggt cgtcgaacac ccgcccgtct ttacgcaagg
    11881 actggcggga aaacccgagc acctgctgat tagggacgac atccccgtcg tccaaatcga
    11941 ccggggcggg cagattacct atcacgggcc cgggcaattg gtcgtttata cgatgattga
    12001 tttcaaacgg cgcaaaacca gcgtccgcaa catcgtttcc gcgcttgaaa acagcatcat
    12061 cgccacattg gcggaatacg gcatcgaagc ggcggcagat cccaaacgcc ccggcattta
    12121 cgtcggcgaa cgcaaaatcg cctcactggg gctgcgtatc aaaaacggtt ccgtctatca
    12181 cggactcgca ctcaacgtca atatggattt aagcccgttt acccaaatca acccctgcgg
    12241 ctacgccggt atggaaatga cgcaaatcgc ggattttgtc caaccctgcc ccgcgccgga
    12301 cgaagtcgcc tccaaactca ccgcacacct tgaaacacaa ctcacaccga aagcagacaa
    12361 caatgagtga aatcaaaacc gacgacccca aacgcggcat caaactcaga ggcgcggaca
    12421 aaaccgcccg catccccatc aaagtcgtcc cccttcagga aaaactgaaa aagcccgaat
    12481 ggatacgcgc caaactccca tcgcgcaaat tctttgaaat caaagacatt ttgcgcgaac
    12541 aaaagatgca caccgtttgc gaggaagcct cctgccccaa catcggcgaa tgcttcagca
    12601 aaggcacggc gaccttcatg attatgggcg acatctgcac ccgccgctgc ccgttctgcg
    12661 acgtgggaca cggccgtccg aatatgctcg accccgacga gccgaaaaac ctcgcagaat
    12721 ccgtcaaagc catgaacctg cgttacgtcg tcatcacctc ggttgaccgc gacgacctgc
    12781 gcgacggcgg cgcccaacac ttcgccgact gcatcaaagc catccgcgaa accagcccga
    12841 acaccaaaat cgaaatcctc gtccccgact tccgaggccg cttggacatc gcactgaaaa
    12901 tccttgccga aaccccgccc gacgtgatga accacaactt ggaaacccat ccgagcctgt
    12961 acagaaaagc ccgtcccggt gccaattatc aacattcttt agacttatta aaacgttata
    13021 aagaaatgat gccgcatatc ccgaccaaat ccggcattat ggtaggcttg ggcgaaacag
    13081 acgaagacgt gcgtgaaatt atgcgcgata tgcgggcgca caatatcgag atgattacca
    13141 tcggacagta cctccagcct tcagacggac acttgcctgt cttgcgctac gttacacccg
    13201 aacaattcaa aatatttgaa aaagaagcat acgaactggg ctttaccaat gccgccatcg
    13261 gcgcgatggt acgttcaagt taccatgccg acgaacaggc ggcggaagcc ttacgcgaaa
    13321 gccacggcgg ttgcgggcat cattaaactc ggaacaccgt tccgaagtaa aaaataattt
    13381 cctacgctgc tgtctgccgc cttgtttgat gtaaacaaca tcaatttgaa ttgtccatgc
    13441 cgtctgaaag tccttcagac ggcataattt attcccaaaa tccgataatc tcaaaagaat
    13501 ctgtcggtta atgagaaaga ttcattataa attttgataa aaaaccgtgc tctatttgtt
    13561 ttttgctatg gttaacaagt gtaaatgttc tttattgatg agatggattc ttagttttag
    13621 cagcaaaaaa aaccgataag aaacagcata ctgatttgga aggcatcggg gaatacgccc
    13681 tatttatttt gataattgtt tgtattttaa atagaaaatt tgtcatgccg acagcttttg
    13741 catacaatgc acgcatcccg aacagcgtta cagaacgccg tttaaccgca cgaaacccaa
    13801 attaagaaaa gagagatatt atgcaaggca atcaagctgt tgttgattac atgaacgaat
    13861 tgctgtccgg cgagctggcg gcacgcgacc aatacttcat ccactcccgc ctctactccg
    13921 aatggggcta caccaaactt tttgaacgtc tcaatcacga gatggaagaa gaaaccacac
    13981 acgccgaaga cttcatccga cgcatcctga tgctgggcgg tacgccgaaa atggcacgcg
    14041 ccgaactgaa tatcggcacg gacgtggttt cctgcctcaa agccgacctg caaaccgaat
    14101 acgaagtgcg cgacgctttg aaaaaaggca tcaaactgtg cgaagaggct caagactatg
    14161 ttacgcgcga cctgatggtt gcccaactga aagacaccga agaagaccac gcacactggt
    14221 tggaacagca gctgcgcctg atcgagctga ttggcgaagg caactactac caaagccaac
    14281 tgtaatccgt attaggaata aaaggagtcc gtatgaaagg cgaccgtttg gttatccgcg
    14341 agctgaacaa aaacttaggc ttgctgctgg taaccattaa ccaatatttc cttcacgccc
    14401 gtattttgaa aaactggggc tttgaagaac tgggcgaaca tttcttcaaa caatcaatcg
    14461 tagaaatgaa agctgccgac gatttgattg aacgcatcct cttccttgag ggattgccca
    14521 atttgcaaga gctggccaag ctgttgattg gtgaatctac cgaagaaatc atcgcctgcg
    14581 atttgaccaa agaacaggaa aaacacgaag ccctgcttgc cgccatcgcc acagcagaag
    14641 cgcaacagga ttatgtcagc cgtgatttgt tggaaaaaca aaaagatact aacgaagaac
    14701 acatcgactg gctcgaaacc cagcaggaac tgatcggcaa aatcggtttg ccgaactacc
    14761 tgcaaacagc ggcgcaagag gactaaaaca caaaccactg ccaaatacag cagtcccctg
    14821 catcatcaga cggcatatcc gtataaacgg gtatgccgtt tttctttgcc ggtttacgaa
    14881 taaaatctaa attgatttga atcaatactc gataacggtt tttgtttgat aataccgata
    14941 tgaaatctag attgtttgtt tgctgtttcc caatccgtct tgattttacc tctttcctct
    15001 tgatgtgtgt gtgtgtttgg gtgtggctgc cgccacccct tttttttggc ttttatgtga
    15061 agtaaaatcc gtaacagcaa atgcttaact tttaaccatt tatccggaaa caaatgatgg
    15121 gcaacaaatt gactctgcct gccgaattgc ctgacgaaca ggatttgcgg gcggtattgg
    15181 catacaatat gcggcttttc tgcgtgaaca agggttggtc gcaagaggaa ttggcgcggc
    15241 aatgcggttt ggacaggact tatgtgtcgg cagtcgagcg caaacgctgg aacattgccc
    15301 tgtcgaatat cgaaaaaatg gcggcggctt tgggcgtggc ggcgtatcag ttgctgctgc
    15361 cgccgcagga acggttgaac ctgatgacca attccgccga tacccgacaa gtgccgtctg
    15421 aaagcggtat ttgaccgccg aaccatccgc ctcccttttt cccgccaatc gaaagaataa
    15481 aaatgcctga aaacctgtct tcagcactgg aaaccttcaa acagcagcgc aatgccgccg
    15541 aagcgcatta tttgaaagcc aaccgcgtgt cggtattttt cagagaatac acggcggcag
    15601 tcgaaacctt gctggcggca ttgtgggcag aacattttca aaacagcgcg ttatgcctga
    15661 tggcggtagg cggcttcggg cgcggcgaac cgtatccctg ttcggatgtg gatttggcgg
    15721 ttgtctcccc tgccccgctt tcagacggca ttcaggaaca gattgcccga tttatccaaa
    15781 ctctgtggga ttgcaaacta atgccgtctg taaaaagcgg cagcgttgac gaactgtgcg
    15841 aaagcgtgcg cgacgatatt acgggggaca cagcgttttt agaggctagg tttttgtttg
    15901 gcaaccgcca aacagcagac gaattggcgg aaaaaatgaa cgtgcaacgc aatgtagcgg
    15961 catttatcga agcaaaattg gtggagatgg aacaccgcca cgccaaatcg caaggttcgg
    16021 gggcggtgtt ggagccgaat atcaaaagct gtccgggcgg tctgcgcgac atccacaccc
    16081 tgctttggat agcgaaggcg caaggcttgg cggccaacct gcccgacctg ctcaaacagc
    16141 ggattttgac acgtgccgaa gccggtatgc tttcgcacgg ctaccgccgc ctcgcccaca
    16201 tccgcatccg gctgcattta aacgccaagc gcgccgaaga ccgcctgctt ttcgatttgc
    16261 agccgcaagt tgccgaaagt atgggttatc aagatgaaaa ccgccgccgt caaagcgaag
    16321 aactgatgcg cgtgttttac cgtgcggtca aaaccgtcaa acaactgggc ggcatcctca
    16381 cgcccatgct gcgaagccgc gtttcctcca cgccggtgcg cgttaccctg cggattgacg
    16441 acgactacat ccaagtcaac aaccaaatcg ccgcgcggca caccgatatt tttttcagac
    16501 ggcccgaaca cattttcaaa atcgtcgaaa tcatgcagca gcgcaacgac attactgcgc
    16561 tcgaaccgca aaccctgcgc gcatggtggg gggcgacgcg caaaatcaac cgcagcttct
    16621 accaaaattc tgaaaaccgt cgccgcttcg ccggtttttt ccgcagcggc aacggactga
    16681 cccaaaccct gcgctttctc aacctctacg gcgtgttggg ccgctatctg cccgcgtggg
    16741 aaaaaatcgt cggcctgctt caacacgact tgttccacat ctatcccgtg gacgaccaca
    16801 tccttgccgt cgtccgcaac gtccgccgcc ttgccctgga tatgcacagc catgagctgc
    16861 cctacgcctc cgcgctgatg cagtcctttg aaaaacaaga catcctctac cttgccgcct
    16921 tcttccacga catcgccaaa ggacgcggcg gcgaccatgc cgtacaaggc atcgcagacg
    16981 cgcgccaatt tgccgccgac cacttcctga ccgaagaaga aagcgacctg ctcgcctggc
    17041 tggttgaaaa ccacctcctt atgtctgccg tcgcccaaaa agaagacatc caagaccccg
    17101 gcgtactcga tgccttctgc aaacgcgtcc aaacccacga acgcctcagc gcgctctacc
    17161 tcctgaccat ttccgacata cgcggcacca atcccaagct gtggaacgcc tggcgcgcca
    17221 gcctgctgga aagcctgttc cacgccgccg gacgctgcct cgcaggcaac gacggcaacc
    17281 cgcacgccct cttcggccgc cgccggcagg aagccgccga cttacttacc cgcgccgccg
    17341 tccccgaaaa acagcagaaa aaactatgga acgcgctcgg ttccgcctat ttcgcccgcc
    17401 atcagtcccg cgaaatcctg tggcacgccg ccaacctcgt acacgacttt gaacccccca
    17461 tcgtccgcag ccgcatccta ccccaaagcg acagctttca agtgatggtc tttatgccca
    17521 acggcccgcg cctgttcgcc cgcctctgcc gcatcttcag ccgccacggc ttcgatatcc
    17581 tcgccgcccg cgcctttatc accgaacacg actacatcct cgacaccttc atcgtgcaaa
    17641 tcccctcgca gcacgccccc gaagactacc ccgacatcca aagcgcgctc gaagccgaac
    17701 tcaacagctt tatccacgga cacaccgttg ccgaaaccca aagctgca
//
LOCUS       DAXFHF010000048        17551 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXFHF010000048 DAXFHF010000000
VERSION     DAXFHF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17551
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.048"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="ACFIRM_001465"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="ACFIRM_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC3029064.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(670..783)
                     /locus_tag="ACFIRM_001466"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="ACFIRM_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="ACFIRM_001467"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="ACFIRM_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC3029065.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="ACFIRM_001468"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="ACFIRM_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC3029066.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="ACFIRM_001469"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="ACFIRM_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC3029067.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="ACFIRM_001470"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="ACFIRM_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC3029068.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFIRM_001471"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFIRM_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC3029069.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="ACFIRM_001472"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="ACFIRM_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC3029070.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFIRM_001473"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFIRM_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC3029071.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="ACFIRM_001474"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="ACFIRM_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC3029072.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFIRM_001475"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFIRM_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC3029073.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFIRM_001476"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFIRM_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC3029074.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="ACFIRM_001477"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="ACFIRM_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC3029075.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="ACFIRM_001478"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="ACFIRM_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC3029076.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="ACFIRM_001479"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="ACFIRM_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC3029077.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFIRM_001480"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFIRM_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC3029078.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFIRM_001481"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFIRM_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC3029079.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="ACFIRM_001482"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="ACFIRM_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC3029080.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="ACFIRM_001483"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="ACFIRM_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC3029081.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFIRM_001484"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFIRM_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC3029082.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFIRM_001485"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFIRM_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC3029083.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="ACFIRM_001486"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="ACFIRM_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC3029084.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="ACFIRM_001487"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="ACFIRM_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC3029085.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRVGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="ACFIRM_001488"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="ACFIRM_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC3029086.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFIRM_001489"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFIRM_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC3029087.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFIRM_001490"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFIRM_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC3029088.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFIRM_001491"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFIRM_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC3029089.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFIRM_001492"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFIRM_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC3029090.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFIRM_001493"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFIRM_001493"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC3029091.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFIRM_001494"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFIRM_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC3029092.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="ACFIRM_001495"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="ACFIRM_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC3029093.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="ACFIRM_001496"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="ACFIRM_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC3029094.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="ACFIRM_001497"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="ACFIRM_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC3029095.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFIRM_001498"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFIRM_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC3029096.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4843 a         3313 c         4329 g         5066 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacgtgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtcaagc ttggtacatt tttgccaagt ttaactttgt ctgaaagaca ggccaatcgc
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa
     1141 gttggtatga cccgcgtgtt cgacgagcag ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagata ctgacggcta taccgccgtt
     1261 caagttacct ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt cggtcaactg
     1441 gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg gcacgatcaa acgtcataac
     1501 ttcggtgccc aacgtacttc ccacggtaac tcccgttctc accgcgttcc aggctctatc
     1561 ggtatggcgc aagacccggg tcgcgtgttc cccggtaagc gcatggccgg ccaatacggc
     1621 aacaccaaag caactgttca aaaattggaa gttgtccgtg ttgatgcaga acgccaactg
     1681 ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcccagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcaccagc
     1861 tggtaaatgc ctacttggca aacgcccgct ctggtaaccg tgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgc gcccgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc cggtatggcg actatcctgt
     2101 cccaattggc gcgtgacgag cgtttgtttg tgattgaggc gttgactgcc gaaactccca
     2161 aaaccaaagt ttttgccgaa caagtaaaaa atttggctct ggagcaagtg ctgtttgtaa
     2221 ccaaacggct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtattggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta taaaaaagta atcatcacta
     2341 aagatgcggt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 aatcttggcg cctgtcgttt ctgaaaaaag caacgtattg gctgaaaaat gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg tcgagctgct
     2521 gttcggtgtt caagttgctt ctgtaactac cgttaccact aaaggcaaaa ctaagcgttt
     2581 tggtcgtatt ttgggtcgcc gcagcgatgt taaaaaagct tatgtaagct tggctgacgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcacc ttatgcaccc ttgcttgaaa agaaaaattc tactgccggc
     2821 cgtaacaata atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggcatttctg ctaaagtaga gcgtatcgaa
     2941 tacgatccta accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgtcgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagcctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gacggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagt tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtcttccag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgtgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg tgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aagttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctact ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggaatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt c
//
LOCUS       DAXFHF010000049        16753 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXFHF010000049 DAXFHF010000000
VERSION     DAXFHF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.049"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            153..1217
                     /locus_tag="ACFIRM_001499"
     CDS             153..1217
                     /locus_tag="ACFIRM_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC3029097.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="ACFIRM_001500"
     CDS             1358..1681
                     /locus_tag="ACFIRM_001500"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC3029098.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="ACFIRM_001501"
     CDS             1755..2078
                     /locus_tag="ACFIRM_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC3029099.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="ACFIRM_001502"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="ACFIRM_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC3029100.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="ACFIRM_001503"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="ACFIRM_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC3029101.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="ACFIRM_001504"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="ACFIRM_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC3029102.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /locus_tag="ACFIRM_001505"
     CDS             3845..4453
                     /locus_tag="ACFIRM_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC3029103.1"
                     /translation="MNTLYLGSGSPCRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="ACFIRM_001506"
     CDS             4462..4815
                     /locus_tag="ACFIRM_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC3029104.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6733
                     /locus_tag="ACFIRM_001507"
     CDS             4805..6733
                     /locus_tag="ACFIRM_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC3029105.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6809..7486
                     /gene="misR"
                     /locus_tag="ACFIRM_001508"
     CDS             6809..7486
                     /gene="misR"
                     /locus_tag="ACFIRM_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC3029106.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /gene="misS"
                     /locus_tag="ACFIRM_001509"
     CDS             7499..8905
                     /gene="misS"
                     /locus_tag="ACFIRM_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC3029107.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11440
                     /locus_tag="ACFIRM_001510"
                     /pseudo
     CDS             9023..11440
                     /locus_tag="ACFIRM_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11485..11730
                     /gene="rpsP"
                     /locus_tag="ACFIRM_001511"
     CDS             11485..11730
                     /gene="rpsP"
                     /locus_tag="ACFIRM_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC3029108.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11746..12255
                     /gene="rimM"
                     /locus_tag="ACFIRM_001512"
     CDS             11746..12255
                     /gene="rimM"
                     /locus_tag="ACFIRM_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC3029109.1"
                     /translation="MTDAQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12255..13004
                     /gene="trmD"
                     /locus_tag="ACFIRM_001513"
     CDS             12255..13004
                     /gene="trmD"
                     /locus_tag="ACFIRM_001513"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC3029110.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13019..13384
                     /gene="rplS"
                     /locus_tag="ACFIRM_001514"
     CDS             13019..13384
                     /gene="rplS"
                     /locus_tag="ACFIRM_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC3029111.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13705..14430
                     /locus_tag="ACFIRM_001515"
     CDS             13705..14430
                     /locus_tag="ACFIRM_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC3029112.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14465..15340
                     /locus_tag="ACFIRM_001516"
     CDS             14465..15340
                     /locus_tag="ACFIRM_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC3029113.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15417..16331
                     /locus_tag="ACFIRM_001517"
     CDS             15417..16331
                     /locus_tag="ACFIRM_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC3029114.1"
                     /translation="MKHLKLTLIAALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16551..>16753
                     /locus_tag="ACFIRM_001518"
     CDS             16551..>16753
                     /locus_tag="ACFIRM_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC3029115.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDMMTDKGKWKLETSLTYLN
                     SENSRSELAAPVYIQTGSASFIPV"
BASE COUNT         4364 a         5327 c         3682 g         3380 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccgcc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagagcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaactgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg caccgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaatt tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caaccgcttg tcgaacacct catcgagctg cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgtgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcga cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcaggattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gctgctcccg gccatccgga
     6601 aacaaaaccc tgcaaacgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggctgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcgg aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gccccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggta aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattacc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctacac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcaacag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgatc
    10561 agttcctgca cacactgacc gtcatccccg aataccgcca acacattttc ggcattaccc
    10621 tcaacggcgg acaatacagc agcgatttca tcttaaagac acccggaaca cacgacacgc
    10681 ccaaacgcaa gaacacaggc aaagccgccc aaaccctcga acatgccgcc gcaaaaatgc
    10741 agagtgccgc cgcatacctg gaacacaaaa acccgacagc cgccgaattt ctccgccaca
    10801 caagcgaagc cgccgcagaa ctgctcggca gcaaaaccga aaccggagca gccgtaccca
    10861 acgtacttgc cccctatccc gcagcacacc ccaaaacact gtccctaaaa aacaacacga
    10921 ccgttaccat tatccccctt ttacccgaag acgcagaagc caaacagcag ttcgtccgca
    10981 gcctcggccc cgaagcacgg tacacacgct tcatgaccca caccaacgaa ctgcccgcag
    11041 ccacgttggc acgcctgtgc aaccccgatt accactgtga accgcatgga cggcaaggga
    11101 tgccgacagc aacatcgtcg ccgtcgtccg ccacagccgc ctgaaccgca acgaatgcga
    11161 atttggcatc acactggcgg aacatatgcg cggcagcggg ctggcacaga aaatgatgga
    11221 actcatcatc caaaccgccg cacagcaagg ctacctgagc atgagtgccg acattctcaa
    11281 aaccaatacc cccatgatca aacttgctga aaaatcagga tttaccctca aggaatcgga
    11341 caccgaaaaa aacctgtacc gcgcatatct gaaccttgcg gcagacaaaa caacaggaaa
    11401 aacaaataaa aacttgcgca ccggccacaa aataacctaa aatcggcagt ttccatatat
    11461 ccgattttct caaaaggact caaaatggta gttatccgtt tggcacgcgg cggctcgaaa
    11521 caccgcccct tctacaacgt catcgttact gactcacgca gccgccgcga cggccgcttc
    11581 atcgaacgcg taggcttcta caaccccgta gccaacgaaa aacaagagcg cgtccgcctc
    11641 aatgcagacc gcctgaacca ctggattgca caaggcgcgc aagtcagcga ctccgttgca
    11701 aaactgatta aagaacaaaa agccgtctaa tccgcgtttg ccgccatgac agacgctcaa
    11761 aaccgggtag ccatgggcta catcaaaggc gtattcggca taaaaggctg gctgaaaatt
    11821 gccgccaaca ccgaatattc cgacagcctt ttggactacc ccgagtggca tttggccaag
    11881 gacggcaaaa ccgtcagcgt tacccttgaa gccggaaaag tcgtcaacgg cgaactccaa
    11941 gtcaaattcg aaggcataga cgaccgcgat tcagcattct cattgcgcgg ttacaccatc
    12001 gaaatacccc gtgaagcatt cgccccgaca gaagaagacg aatactactg ggcagacttg
    12061 gtcggcatga ccgtcgtcaa caaagacgat accgttttag gcaaggtaag caacctgatg
    12121 gaaaccggcg caaacgacgt attgatgatt gacggagaac acgggcagat tctgattccg
    12181 ttcgtttccc aatatatcga aaccgtcgat accggcagca agaccattac tgccgactgg
    12241 ggtttggact actgatgctt atccaggcag ttaccatttt ccccgaaatg ttcgacagca
    12301 ttacccgcta cggcgtaacg ggacgcgcga acagacaggg aatctggcag tttgaagcag
    12361 tcaatccccg aaagtttgcc gacaacagat tgggctatat cgacgaccgc ccgttcggcg
    12421 gcggtccggg aatgattatg atggctccgc cgcttcatgc ggcgatagaa cacgccaaag
    12481 cacaatcttc ccaaaccgca aaagtcatct acctcagccc ccaaggaaaa ccgctgacac
    12541 accaaaaagc ggcagaactg gcagaactta cgcatctgat tctgctgtgc ggacgctatg
    12601 agggaataga cgaaagactg ctgcaaagca gcgtcgatga agaaatcagc atcggagact
    12661 tcgtcgtttc cggcggagag cttcccgcca tgatgctgat ggatgcggta ttgaggctcg
    12721 tacccggcat attgggcgac attcagtctg ccgaacagga ttcgttctca agcggtattt
    12781 tggactgccc ccactacacc aaacccttag aatttcaagg catggctgtt ccggaagtat
    12841 tgcgctccgg aaatcatggc ttgatagcgg aatggcggtt ggaacaatcg ctgcgccgca
    12901 ccttggagcg cagacccgat cttttggaaa agcgcgtttt aatcccaaag gaatcccgcc
    12961 tcttgaataa aatcctacaa gagcaacggg aaatccaatc ataatttagg aaaaaacaat
    13021 gaacctgatt caacagctcg agcaagaaga aattgcccgc ctgaacaaag aaatccccga
    13081 attcgcaccg ggcgacaccg tagtcgtatc cgtacgcgtc gtggaaggta cccgcagccg
    13141 tctgcaagcc tacgaaggcg tggttatcgc ccgtcgcaac cgtggtttga acagcaactt
    13201 catcgtccgc aaaatctcca gcggcgaagg tgttgaacgt actttccaac tgtattcccc
    13261 tactgttgag aaaatcgaag tcaaacgccg tggcgacgta cgccgtgcca aactgtacta
    13321 cctgcgcggt ctgaccggca aagctgcacg catcaaagaa aaactgcctg cacgcaaagg
    13381 ttgattcaaa ccgttttccc ccaatgccgt ctgaacgttc agacggcatt ttttattgcc
    13441 gcgatgccgg caaggcaacg atatgcctga ccctcatcca ccttaattaa caatcactcc
    13501 cgcctattca agcagaattt acagactaaa cgttatacag tatcatatcc gccatataac
    13561 gataaccgct ttgccaactt ccctttcaaa actgcaaatc attatgacac cttccctctt
    13621 actgtcagga ttgaccttcc gactcttcct cgccctgatt gccgtattcc ttttatgggg
    13681 tgtttacctt tgggcggtat cggtatgagc atcatcgttg aaaacctgac ggtcagctac
    13741 cgccgccgcc ccgccgtaca ccacgtcgac attacttttg aagaacatag tatgtgggcg
    13801 gttttcggtc ccaacggcgc aggaaaatct acctttctca aatccttaat gggattgcag
    13861 cctatcgata cgggcagcat ccggctggac ggattgacca gtcaaaacat tgcctatctc
    13921 ccccaacaat ccgacatcga ccgttcccag cctatgaccg ttttcgactt ggcggcaatg
    13981 gggctgtggt atgaaatcgg ttttttcaaa gggataaata ccgctcaaaa acaacgcgtt
    14041 cacgaagcct tggagcgtgt cggtatgcgg cagtttgccc gccgccaaat cgcccacctc
    14101 tcaaacggac aattccaacg cgtccttttc gcccggatgc tggttcaaaa tgcaaaattc
    14161 ctgcttttgg acgaaccttt caatgctgtt gatgcacgga caacctacga gcttctcgac
    14221 gtgttgcaaa aatgccattg cggcggacac gctatcattg ccgttttgca cgattacgaa
    14281 caagtccgtg cctactttcc caataccctg ctgctcgccc gcgaaaaaat tgcggcaggc
    14341 gcaaccgaga ccattctgac agaatcctac ctcgcccaag ccagcgccaa aatgcagcaa
    14401 caggaaagcc ccgactggtg cgcctcataa atactgcccg aaaccacgcc ccacaccaga
    14461 caccatgaac ctctacgacc tgctccttgc cccctttgca gaattcgact tcatgcacta
    14521 cgccctcgca tccgtcttct gcctgtccct cagcgccgca cccgtcggcg tattcctcgt
    14581 catgcgccgt atgagcctga taggcgacgc attgagccac gccgtcctgc ctggtgccgc
    14641 cgtcggctac atgtttgccg gcttgagcct gcccgctatg ggtgtgggcg ggtttgccgc
    14701 cggtatgctg atggcgctgc ttgccggact cgtcagccgc tttaccaccc tgaaagaaga
    14761 tgccaacttt gccgcctttt acctgagcag cctcgccatc ggcgtaatcc tcatcagcaa
    14821 aaacggcagc agcgtcgatt tactccacct ccttttcgga tctgtgcttg ccgtcgatat
    14881 tcccgcactg caactcatcg ccgccgtctc cggcctcacg ctcattaccc ttgccgtcat
    14941 ctaccgcccc ctggtgctag aaagcataga ccccctgttc ctcaagtccg tcaacggcaa
    15001 aggcgggctt tggcacgtcg ttttcctcat cctcgtcgtt atgaacctcg tatccggctt
    15061 ccaagctctc ggcaccctga tgtcggtcgg acttatgatg ctgcccgcca ttaccgcccg
    15121 cttatgggca agaaatatgg ggacgctcat tctgttgtcc gtcctcatcg cccttttttg
    15181 cggtttgatc gggctgctca tttcctacca catcgaaatc ccttccggcc ccgccatcat
    15241 cctctgttgc agcgtccttt atctcttttc cgtcatactc ggcaaagaag gcggcatctt
    15301 gcccaaatgg ttcaaaaacc accgccacca caccacctga ccccgcaagc cgcttgaaca
    15361 aacaggcggc ttgttttacc ataatgaatg ttatgttata tcacaggaaa acagttatga
    15421 aacacctcaa acttaccctt attgccgcat tgctggccac cgccgcaact gccgcacccc
    15481 ttccggttgt aaccagtttc agcattttag gcgacgtagc caaacaaatc ggcggtgagc
    15541 gcgtagccgt acaaagcctc gtcggagcca accaagatac tcatgcctat cacatgacca
    15601 gtggcgacat taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc ttgggacttg
    15661 aagccgccga catccaacgc gccgtcaaac agagcaaagt atcctatgcc gaagcgacca
    15721 aaggcatcca acccctcaaa gccgaagaag aaggcggaca ccatcacgac cacgatcatg
    15781 accacgaagg acaccaccac gaccacggcg aatatgaccc ccacgtctgg aacgaccctg
    15841 ttcttatgtc cgactatgcc caaaacgtcg ctgaagccct gataaaagcc gatcccgaag
    15901 gcaaagttta ttatcaacaa cgcttgggca actaccaaat gcagcttaaa aaactgcaca
    15961 gcgacgcaca agccgcattt aatgccgtcc ctgccgccaa acgcaaagtc ctgaccgggc
    16021 acgacgcatt ttcctacatg ggcaaccgct acaacatcag cttcatcgcc ccgcaaggcg
    16081 tgagcagcga agccgagccg tccgccaaac aagtcgccgc catcatccgg caaatcaaac
    16141 gcgaaggcat caaagccgta tttaccgaaa atatcaaaga cacccgcatg gttgaccgca
    16201 tcgccaaaga aaccggcgtc aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg
    16261 cgcccgcaga cacctacatc ggcatgtacc gccacaacgt caaagccttg accaacgcga
    16321 tgaagcaata aaaccgtttg attggtttga atttgaaaag gcaaaaggcc gtctgaaaat
    16381 taaatacgag gttttcagac ggccttctct tgtgcaataa agcagtaaac aaatattgat
    16441 aagaaaaaaa caatcagtat tgaaaatatc gttaaaaata ttatttagac agattacatg
    16501 gaatttcatt aacatcagta gactttgatt tcaatcaaga aaacaatgtc atgaaaaata
    16561 ttctcttagt atttgttagc tttgtgccat tatgtgtccg cactgatctg ccgctgaata
    16621 tcgaagacat gatgaccgac aagggaaaat ggaaactgga aacttccctt acctacctga
    16681 atagcgaaaa cagccgttcc gaacttgccg caccggttta cattcagacc ggctccgctt
    16741 cctttatccc cgt
//
LOCUS       DAXFHF010000050        16642 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXFHF010000050 DAXFHF010000000
VERSION     DAXFHF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.050"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFIRM_001519"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFIRM_001519"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC3029116.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="ACFIRM_001520"
     CDS             complement(734..1447)
                     /locus_tag="ACFIRM_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC3029117.1"
                     /translation="MKTKLPLFIIWLSVSASCASVSPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="ACFIRM_001521"
     CDS             complement(1447..2115)
                     /locus_tag="ACFIRM_001521"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3029118.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="ACFIRM_001522"
     CDS             complement(2126..2311)
                     /locus_tag="ACFIRM_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC3029119.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="ACFIRM_001523"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="ACFIRM_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC3029120.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFIRM_001524"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFIRM_001524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC3029121.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTVSEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="ACFIRM_001524"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="ACFIRM_001525"
     CDS             6730..7065
                     /locus_tag="ACFIRM_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC3029122.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFIRM_001526"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFIRM_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC3029123.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /gene="aroD"
                     /locus_tag="ACFIRM_001527"
     CDS             8964..9728
                     /gene="aroD"
                     /locus_tag="ACFIRM_001527"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC3029124.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="ACFIRM_001528"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="ACFIRM_001528"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC3029125.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFIRM_001529"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFIRM_001529"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC3029126.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
     gene            12990..13199
                     /locus_tag="ACFIRM_001530"
     CDS             12990..13199
                     /locus_tag="ACFIRM_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029127.1"
                     /translation="MKLDKNNGFYQAERNSNGNYDIVRKNYLKNITKIQSNILIYLDN
                     TSTGFKIQQRRKQIRAQISARQWRR"
     gene            13791..14570
                     /locus_tag="ACFIRM_001531"
     CDS             13791..14570
                     /locus_tag="ACFIRM_001531"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3029128.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            14779..15849
                     /locus_tag="ACFIRM_001532"
     CDS             14779..15849
                     /locus_tag="ACFIRM_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3029129.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKPQPHIRPDELPDF
                     YRTLQTAHDLHPYARPLLLLAALTVPRPSALLSARWQDIDLTARLWHIPAADMKTKHP
                     FTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYD
                     GSTPANPNTPCTDSATSSPTSA"
     gene            15810..16058
                     /locus_tag="ACFIRM_001533"
     CDS             15810..16058
                     /locus_tag="ACFIRM_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029130.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            16078..16341
                     /locus_tag="ACFIRM_001534"
     CDS             16078..16341
                     /locus_tag="ACFIRM_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029131.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            16378..>16642
                     /locus_tag="ACFIRM_001535"
     CDS             16378..>16642
                     /locus_tag="ACFIRM_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029132.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
BASE COUNT         4251 a         4934 c         4098 g         3359 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggggtacgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtgtttttgc gtgttccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtgaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 aatccgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcaggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caattacttg gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggtatccga agccgaaacg ccgcccgaaa atcaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaagcca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtaatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atgcccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgaaca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccagcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cagacaatgg agaagataaa
    13201 agatgaataa aaatataatt tatatatttt ctttattaat cactattgtt attttcttta
    13261 tatttgaaaa gaatgtaata agcaaaataa gttttgatta taataaaaaa gaattcttaa
    13321 ttagtgatat aacgaacttt aactgggatc atgtaaaact gtatataatc aattcagatt
    13381 tccaaaaaat tgttttttat gataaaggta agatagtttt tgaggaatta atagaactag
    13441 ataggaaagg gaaagtttta cctcaatatc tatttgattc atatttgaaa aatgtttaat
    13501 attacgaatg tgattacaag aatggcaaga tgcaactttt aaaaaagaaa aattacattc
    13561 ttttgataga tatttttatt attataaacc tataaactgt aggccgaaac ttttgtaaaa
    13621 ctgtccatac tcattaccgc ctagtcggta atgcattttt tagtagtatt tgaaaaagta
    13681 tgatgataaa aacggttaag cctaactttt tcataaaatc cagcagagta aggcagaaaa
    13741 aaacacccaa attggctata atcccgacaa acacactcaa ggacaacaac atggcagcct
    13801 cgcccgaagc aaaattcacc gaagaaaaga ttttgtgggt caaacaccac acgccgaaac
    13861 tcatcacttt cgccatcagc cgtcccgaat cctaccgttt taaagccgga cagttctccc
    13921 gactcggttt ctacgaaggt aaaggcttta tttggcgtgc ctattccgtc gtttccgcag
    13981 aatatgccga cacgctcgaa tattttgccg tactcatcca agacggcccc atgtcggccc
    14041 ttttcgccaa aatgcaacag ggcgacacca tcctgctcga taaaaatgcc actggctttc
    14101 tcctgcccga acgcttcccc gacggcaagg atttggtgat gctctgtacc ggctcgggca
    14161 tcgccccctt cctttccatt ctcgaacaac ccgaaatccg gcagcgtttc gataccgtca
    14221 acctgataca ttccgtatct tttcccgaag aattgatttt caacgaccgg ctggccgcat
    14281 tgtccgaaca tcccctggta ggcgaatacg gacactcgtt ccgtttcgtc cctgttacca
    14341 cccgtgccgc caacccctcg ggcttaagcg gaaaacgcat tccggaactc ttaaaaaaca
    14401 acagcatcga acaggcgctg cataccaagc tcaccccgga atccacacgg tttatgattt
    14461 gcggtaaccc ggaaatggtc aaagacactt tccaaacgct gctcgatatg ggttacgcca
    14521 tgcaccgcaa ccgcattccc ggtcaaatca tgatggaaaa cggcttctaa aaacgaacgc
    14581 tcccaacccc tacccacgcc cacacacaaa acaaaataaa aaacagcgat atacaaaaac
    14641 caatttgcaa aaacagacct cacgcctata ctgccactca cagcaagcaa cacaaaaaag
    14701 cgggtacaaa ttagggtaca aaacacaagc ccgaacacag aagcacccca accaaaccca
    14761 aaggcaaagg caaacaaaat ggcggcacac aacaaactga cccaaaaaca aatagaagca
    14821 gccaaggcag acggcaaaca aagcaagctg gccgacggcg gcggcttata tctgctgctg
    14881 caccccaacg gcagcaaata ctggcggatg cgctaccggc acggcggatg cgaaaaaacc
    14941 ctagccttgg gcgtatatcc cgccgtcagc ctcaagcagg cccgcgaact tgcccgcgcc
    15001 gcccgggcgc agaccgccgc cggcatcgac cccgtcgccg aacaccaccg cacccgtccc
    15061 ggcagcgggc gcagccttcc cgaaatcgcc cgcgcgtggt acggcagccg tcaagggcaa
    15121 cgcgccgaca acacactgga aggcgaccgc cgttcactcg cctacctgac cgactactac
    15181 aaagacaaca ccgacatcaa cgacatcacc accgccggcg tatcaaaatt tatcgaacac
    15241 ctcaaccgca tcaacatccc cgcctgcgcc cggcgcaccg tccaaatcct cgcccaaata
    15301 tgggactatg ccgtccagcg cggcatcatt accgacgaac ggcgcaaccc cgcaaccacc
    15361 gcccgcccac tgctaaacac aagcaaaccc aaaccccagc cccacatccg ccccgacgag
    15421 ttgcccgact tttaccgcac cctccaaacc gcccacgacc tgcacccgta cgcccgcccc
    15481 ctgctgctgc ttgccgccct gaccgtcccc cgtccgtccg cactgctttc cgcccgctgg
    15541 caagacatcg acctcaccgc ccggttatgg cacatccccg ccgccgacat gaagaccaaa
    15601 caccccttta ccgtcccact gtcggactgg gcagtagaaa tactccgaga gctgcacaca
    15661 cagaccggcg acaacatcca ccttttccca ggcatccgcc cacggcgcaa acccgccccc
    15721 catcccgacc acatcagcat caaattcgcc cacaacgcca tccgacgact gggctatgac
    15781 ggcagcaccc cggcaaatcc aaacacacca tgcacggatt ccgcgacctc ttcaccaaca
    15841 tcagcctgac cgccggaaaa gacaccctca caacagacct cgcccttggc cacatcagcc
    15901 gcgccgccct ccaacgcgcc ggcttctcaa gcctgcacca ctacctgacc gccgacagct
    15961 accgcctcca agagcggcgc gaactcgccg aatggtacgg ccgccgccac cgccaagcat
    16021 acgaagccgc cgcccaaaac acttgcaata aaacctaaaa ggtattaaca tacatacatg
    16081 gcagacatcc tgccatccga caagcggcgg caaccgccaa aaacgaaagg aaatccaata
    16141 aacataatcg aacaagaaac aaaacgcacc gagttaagaa aaatagatgc cgaaatcgcc
    16201 aaaatcattg ccgacgcgca caaaatcaac gccgaatccg taaaaatagc ccaagagtcc
    16261 cgctggtatc ccatgatggc ggccaccggt ctggttaccg ccatcgccgc cgtgttggca
    16321 ctgatattca aatttgcctg accacaagcc ccgcgagggg ctttatccct caacaaaatg
    16381 aacagagccg cactattcaa ccgctacccc gaatggatta tcgggcaaga cggcgcaagc
    16441 cgtttcatta cccattgccg ctatccccgc ctgattgcca aaatccacag gcagacagac
    16501 ggcgaatgcc cgggcgggca ttaccgccat tccgaaaacg gcatcaccct ttacgatttt
    16561 atttttttcg gcggcaaacc ggcagacgaa gcccgatttg ccgccgtcct taccgaaacc
    16621 tgccgccggg cagtcaaaaa aa
//
LOCUS       DAXFHF010000051        16526 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXFHF010000051 DAXFHF010000000
VERSION     DAXFHF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.051"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            524..2059
                     /locus_tag="ACFIRM_001536"
     CDS             524..2059
                     /locus_tag="ACFIRM_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3029133.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTAWVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            2056..2148
                     /locus_tag="ACFIRM_001537"
     CDS             2056..2148
                     /locus_tag="ACFIRM_001537"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC3029134.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(2200..2622)
                     /locus_tag="ACFIRM_001538"
     CDS             complement(2200..2622)
                     /locus_tag="ACFIRM_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC3029135.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRDGHIVRAQTLDASGKVEREWQP"
     gene            complement(2816..4036)
                     /gene="lysA"
                     /locus_tag="ACFIRM_001539"
     CDS             complement(2816..4036)
                     /gene="lysA"
                     /locus_tag="ACFIRM_001539"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC3029136.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASFGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(4047..4217)
                     /locus_tag="ACFIRM_001540"
     CDS             complement(4047..4217)
                     /locus_tag="ACFIRM_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC3029137.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            4288..4611
                     /gene="cyaY"
                     /locus_tag="ACFIRM_001541"
     CDS             4288..4611
                     /gene="cyaY"
                     /locus_tag="ACFIRM_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC3029138.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            4644..5063
                     /locus_tag="ACFIRM_001542"
     CDS             4644..5063
                     /locus_tag="ACFIRM_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC3029139.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            5085..5591
                     /gene="luxS"
                     /locus_tag="ACFIRM_001543"
     CDS             5085..5591
                     /gene="luxS"
                     /locus_tag="ACFIRM_001543"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC3029140.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            5735..8527
                     /gene="polA"
                     /locus_tag="ACFIRM_001544"
     CDS             5735..8527
                     /gene="polA"
                     /locus_tag="ACFIRM_001544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC3029141.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            9089..9784
                     /locus_tag="ACFIRM_001545"
     CDS             9089..9784
                     /locus_tag="ACFIRM_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3029142.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INDKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(9776..9886)
                     /locus_tag="ACFIRM_001546"
                     /pseudo
     CDS             complement(9776..9886)
                     /locus_tag="ACFIRM_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10024..14430)
                     /gene="app"
                     /locus_tag="ACFIRM_001547"
     CDS             complement(10024..14430)
                     /gene="app"
                     /locus_tag="ACFIRM_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC3029143.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTGNTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            14868..16214
                     /gene="mnmE"
                     /locus_tag="ACFIRM_001548"
     CDS             14868..16214
                     /gene="mnmE"
                     /locus_tag="ACFIRM_001548"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC3029144.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
BASE COUNT         3641 a         4222 c         4549 g         4114 t
ORIGIN      
        1 accggttccg taccatttgt actgcctgcg gctggccgcc ttgtcctgat ttttgttaat
       61 ccgctatatt tcccgccatc ccaaaaacga agagcggcag gaatttatcg gaaaaacagc
      121 aacctttccg ccgtcattcc cgcgaaagcg ggaatctagg ttcgtccggt ttcggttatt
      181 tccgatagat tcctgccgcg ttgggggtct ggattcccgc ctgcgcggga atgacgggac
      241 tttaggtttc tgtttttgtt tttctgtttt tgcgggaatg acggggaagt tggcggttgt
      301 ttcttatgga gtggtttttg tatttggata aaaaattttt cgttttcaag ccttcaccgc
      361 ttgccatcgg cgttaaaatt ttttacgata agcacataga ttgtaaacaa tcggccacaa
      421 gccggtttgt tttttcagaa gacattatcc ctgtcagacg ctgtttctat atatgtttgc
      481 ctataacggc ttgtttttaa taaataattc aagaggtatc aacgtgtctg attccaaaac
      541 gaaagaacgc gccacattcg gcacgcgccg cgcgtttatg attgccgcca tcgggtccgc
      601 cgtcggcttg ggcaatattt ggcgtttccc ctatattgct tttgaaaacg gcggcggcgc
      661 gttcatcctg ccctatctgg tcgcgcttct gacggcgggc atcccgctgc tgctgctcga
      721 ttatgccatc ggccaccgtt accggggttc tgcgcccttg gctttccgcc gcctcggacg
      781 ctggttcgag ccggtcggct ggtggaacgt gatgaccaat atcgtcatct gcatctatta
      841 cgcagtgatt atcggttggg cggcaagcta tacctattat tcggtcaacg ccgcctgggg
      901 tgcggatccg cagggttttt tctttaagga cttcctgcaa atggcgggcc cggaagcctt
      961 gggtttggat tttgtcggca aagtcgccgg ccctttggcg ggcgtgtggg tttttaccgc
     1021 cgccattatg gctttgggcg tgcaaaaggg cgtggcgcgc gcctcgtcgt tctttatgcc
     1081 gctgcttttg gtgatgtttt tgattatggt cggcatttcg ctgacgctgc cgggtgcggc
     1141 aaagggcttg gacgcattgt ttacgcccga ctggtcgaaa ctcgccgatt ccaaggtctg
     1201 ggtggcggca tacgggcaga ttttcttttc gctttccatc tgcttcggca ttatggttac
     1261 ctattcttct tatttgaaga aaaaaaccga cttgggcgga acggggctgg tggtcggttt
     1321 tgccaacagc agcttcgaac tgctcgcggg catcggcgtg tttgccgcat tgggctttat
     1381 ggcgcaggcg ggcggcaagg cggtcaacga ggttgcctcc ggcggcatcg gtttggcgtt
     1441 tatcgccttt ccgaccatta tcaaccaggc gccgatgggc tggctgatcg gcatattgtt
     1501 tttcggttcg ctggtgttcg ccggcgttac gtcgatgatt tccatccttg aagtgattgt
     1561 ggcggcgatt caggacaagc tgaacatcgg gcgcgtcaac gccacgctgc tggtctgcat
     1621 tccgatgggc attgtttcca cgctgctgtt cggtacggcg acggggctgc ctgttttgga
     1681 cgtgatggac aaattcgtca acacctacgg cattgttgcc gccggctttg tttatgttgc
     1741 cgccatcatc atcggcggca ggttgccgga attacgcagg cacctgaacg ccttgtcctc
     1801 catccgcgtc ggcggcttgt ggacggcctg ggtcgtgttt accgtcgtga tgctcggcta
     1861 tatgctgtat caggacaccg ccggattgtt ggaaaaaaac tacggcgatt atccggatgg
     1921 tttcctcaat attttcggct gggggatgtc ggcggcgtta atcatgttcg ggctgctgct
     1981 gtcgctgctg ccttggaaac acggtcagga ttttaatgtc aaagacgaac acgaacatga
     2041 acaaggagga gaaaaatgag tacttccgcc attgtgatga tggttgccgc catcgcggtg
     2101 atttggggcg ggctgctgct ttccctgtta aggctgccgg aagagtaagc ctttagaacg
     2161 ttaaaaatgc cgtctgaacc gcttcagacg gcatttgttt tacggctgcc attcgcgttc
     2221 caccttgccc gacgcatcca aagtttgcgc tcggacgata tgcccgtccc ggtacaggat
     2281 gcgcgtccgc aatatgccct gttcggtata gccttgcgcc acgccgtttt caaccggcgt
     2341 gtgcgaatgc agtttgccgt ttggatggta aatatcgaca aagcggtcga acacgccgtt
     2401 tttgacgatg cttttcaaga cgcgcccgtt tgaggcgcgg gtgctgaatg cgccgtccgg
     2461 cgtgcctgaa ggcggccggc tgtatgggtt ggcgggttgg atgggcgcgc ttttcggcag
     2521 ggtgcgaagc ggcggccggt cagagggaag ggcggtgcag cccgccgaaa gtagggcggc
     2581 ggacgaagcg gtaagcagga ggataatcgg agcgtgcttc atttcaattc cttagatatt
     2641 gcaccccata aaaggagcgc gtaatttttt tcaacaattc ccttcatcag tcgggcgaga
     2701 cggggatttg ttgcgtcgtc atttgaaaac aggctgtgtt tgaatattcc gccaagatgt
     2761 cactttaaac ggacaatgcc gtctgaaggc ttcagacggc attttgatgt tcggcttata
     2821 ggcaggcgag ttcgtttgcc atttgctgtt ccaaggtttc gcgccggcgg atgagtttgt
     2881 atccgccgcc gtcaaccaac acctccgccg cacggttgcg cgtgttgtaa ttgctagcca
     2941 tactggcccc gtatgcgccc gcgctgcgga taagcagcaa atcgccttct tcgcaggcga
     3001 tggtgcggtc tttgccgagg aagtcgccgg tttcacaaat cgggccgacg atgttggcgg
     3061 tcagaggctc aatgtttttg gtttcaaccg cttcgatgtg gtggtaggca tcgtataggg
     3121 ctgggcgcat caaatcgttc atcgccgcat cgaccatcac aaagtttttc tcttcaccgt
     3181 gtttgacaaa ttcgacgcgc gtcagcaatg cacctgcgtt gccgaccaag ctgcggcctg
     3241 gctcaagaat gagtttcaga cggcgtgtcc ccatcagttt ttgaaccgct cgggcatacg
     3301 cacccaaatc ggggacgcct tcgtctttgt aaacaatgcc gacgccgccg cctaagtcca
     3361 aatgttccaa aacaatgcct tcggcggcaa gagcgtcaac caaaatcaaa atgcgttcgc
     3421 aggcttcgac cagtgggctt aagtcggtca gttgcgaacc gatgtggcag tcgatgccga
     3481 tgattttcaa attgggctgt tgtgcggcat ggcggtaggc ttcgagcgcg tcggcgtagg
     3541 cgatgccgaa tttgttggct ttcagacctg tggagatgta gggatgggtt tttgcatcga
     3601 catcgggatt gacgcgcagg gagacgggcg cggttttgcc caaacgcgcg gcaattttct
     3661 gaatgcggtc gatttcgggg atgctttcca tattgaagca ttttacgccg gcattcagcg
     3721 cgaactcgat ttccgcctcg cttttgccta cgccggaaaa aatcgttttc gccgcatcgc
     3781 cgcctgccgc caaaacgcgt gccaattcgc cgcccgacac aatgtcaaaa ccgctgccaa
     3841 aagaagcaaa gtgtttgata atgctcaggt tgccgtttgc cttgacggcg tagcagacaa
     3901 gcgggttcaa agcggcaaag gcggtttgat agttttcaaa tgctccggtc agcgcggatt
     3961 ggctgtacac ataaagcggc gtgccgaatt cttcggcaag gcgggggtag gggacttgtt
     4021 cgcaaaacag ggtcatattt tcgttttcat ttttgggttg gtggggcgga ttgcggtttg
     4081 ccttgaagct gcaaaccggt ttggatcacg ccgaaacgcg ccgtatcgcc ttctttgggc
     4141 aggtagaggc cgcctttgta accgcaggcg gaaagcagga gggcggttgc cgccgcaaaa
     4201 aatacgccgt atttcatcgg taaacttcct tcataagcgc gaatgtggca agattcggca
     4261 tcttaaacaa aaaacatgca aaaagctatg atgaccgaaa gcgagtttat ccgcgcgagc
     4321 gaagcattat ttgaacacat cgaagaccaa atcgacgaaa acggctggga tttcgactgc
     4381 cggtttgccg gaaacgtcct gaccatcgaa gccggagacg gcacgcaaat catcgtcaac
     4441 cgccacacgc ccaaccaaga attgtggatt gccgcaaaaa gcggcggcta ccatttcgcc
     4501 gaacaaaacg gcaaatggct ggcaacgcgc gacagccgcg atttttacga cgttttaaac
     4561 gaagccctga gcgcggcttc gggcgaagcg gttgagattg ccgaattgtg attcaatttt
     4621 cgatataaaa agattgttac ccaatggcac aattacctct atatcggact gccgaaatcg
     4681 gaaattttac tgtcggcacg cctaaagttt tagaatcatt ttccaaacat atcccttatg
     4741 gtgttgtctt tgaagatgat ggcaacacgg gctacttcta tgccgcttcg caagagggga
     4801 ttttggatgc cttgcacatc tacaatgtcg aagatgtatc cgacaaacat atccccaatc
     4861 atgtcttgat tttatgggat ggcgcctgca ccatagccgc attgtgtatc aacgactaca
     4921 tccatgccgt ctatgatttt gtcgaacagg caggatattg ccgcaacggc ttccctgaag
     4981 caggcggcga atgggtaaaa gtcgaaaacc gcgtcttgga cgatgaattg ctggacaaaa
     5041 tcctatcccg aaaacctaca taacccgcac aaaaggatac ccaaatgccc ctgttagaca
     5101 gtttcaaagt cgatcacacc cgtatgcacg cccctgccgt acgcgtggcg aaaaccatga
     5161 ccacgcccaa aggcgacacc attactgtat tcgacctgcg cttttgcatc cccaataaag
     5221 aaatcctgcc cgaaaaaggc atccacacgc tggagcattt gtttgccggc tttatgcgcg
     5281 accacttgaa cggcgccggc gtggaaatca tcgacatttc cccgatgggc tgccgcaccg
     5341 gtttttatat gagccttatc ggcacgcctt ccgaacagca ggttgccgat gcgtggttgg
     5401 cttcgatgca ggatgttttg aatgtcaaag accaaagtaa aatccccgag ttgaacgaat
     5461 accaatgcgg cacttatctg atgcactcgc tcgccgaagc gcaacaaatc gcgcaaaacg
     5521 tgttggcgcg caaagtggcg gttaaccgaa acggcgattt ggctttggat gaaagcctgc
     5581 tgaacgctta atccgccgca aatgctgtct gaacaagggt ttcagacggc atttgccttt
     5641 tccgttataa tccggggttg tacgggggcg ggttttaagc cggcatcgtc cttccctatt
     5701 tttctgtccc ttatcggttt caagcgggtt ttttatgtcc aacagaccta cactcctcct
     5761 cgttgacggc tcttcctatc tctaccgcgc gtatcacgcg atggggcaaa acctgactgc
     5821 gcctgacggt gcgccgacgg gtgcgctgta tggcgtattg aatatgttgc gccgtttgcg
     5881 gtcggaatat ccgcacgatt attgcgcggt ggtttttgat gcgaaaggca aaaatttccg
     5941 ccatcaaatg tttgaagaat acaaggcgac gcgcccgccg atgccggacg atttgcgccc
     6001 gcaggcggaa gccctgccgg atttggtgcg cctgacgggc tggccggtat tggtgattgg
     6061 gcaagtggag gcggacgatg tgatcggcac gctggcgaaa cagggggcgg aacatggttt
     6121 gcgagtcatt gtttcgacgg gcgataagga tatggcgcag ttggtggatg agcgcgttac
     6181 gctggtgaac acgatgagcg gagaaacgct ggatattgaa ggcgtgaagg caaaattcgg
     6241 cgttcgcccc gaccaaatcc gcgattatct cgcgctgatc ggcgacaagg tggacaacgt
     6301 accgggcgtg gaaaaatgcg gcccgaaaac ggcggtgaaa tggctggaag cctacggttc
     6361 gttgcaaggc gtgatggaac acgctccgga aatcaaaggc aaggtcggcg aaaacctgca
     6421 agccgcgctg ccccaactgc cgctgtcgta tgatttggtc acgattaaaa ccgatgtgga
     6481 cttgcacacc gagctttcag acggcatcga aagcctgcgc cgtaccgcgc cgaaatgggc
     6541 gcagcttgcg gttgatttca aacgctgggg cttccgtact tggctgaaag aagcggaatc
     6601 aaacatgaat accggctcga ccgatgattt gttcggtagc gacagcatcg gcgagcaggc
     6661 ggctttgaat gcggaaatac cgtctgaaaa acgagctgaa aaagccaccg cccctgaaaa
     6721 actggattat caagccgtta ccaccgaagc tcagtttgcc gccttgttgg acaaactagc
     6781 gaaggcggac acaatcggca ttgatacgga aaccacgtca ttagatgcga tgaatgccga
     6841 gctggtcggc atcagcattg cgttccaagc aggcgaagcg gtttacatcc ccgtaggcca
     6901 cagcctgact gccgcgcccg aacagcttga tttgcaagac gtattaggcc gtctgaagcc
     6961 gcatttggaa aaccccgccc tgaaaaaaat cggtcaaaac ctcaaatacg accaacacgt
     7021 tttcgccaac tacggcatcg cgttaaacaa cattgccggc gacgcgatgc tcgcttccta
     7081 catcatcgag agccatctcg gacacggctt ggacgaattg tccggacgct ggctcggttt
     7141 agaaaccatt acctacgaat cgctgtgcgg caaaggcgcg aagcaaatca gttttgccga
     7201 tgtcgccatc gggcaggcga ccgaatacgc cgcccaagat gccgatttcg ccctgcgcct
     7261 cgaagcgcgc ctgcgcgcgc aaatggacga caaacaactt gaaatgtatg aaaaaatgga
     7321 gcttcccgtc gcgcaggtat tgtttgaaat ggaatgcaac ggcgtgcaaa tcgaccgcgc
     7381 cgaactcgcc cgccaaagcg cggaactcgg cgcggagctg atgaagctcg aacaggaagc
     7441 ctacgccgct gcaggtcaac cgtttaacct caactcgccc aaacagctgc aagaaatcct
     7501 gttcgacaaa atgggcatcc cgacaaaagg gctgaaaaaa accgccaaag gcggcatttc
     7561 caccaacgaa gccgtgctcg aacagctcgc gcccgactac cccctgccca aaatcatcct
     7621 gcaaaaccgc agcctggcga aactcaaatc cacctacacc gacaaactgc ccgaaatgat
     7681 ttcgccccgg gataaccgcg tgcataccac ctatgcccaa gccgtcgcca ttaccggccg
     7741 cctcgccagc aacaacccca acctgcaaaa catccccatt cgcaccgccg aaggccgccg
     7801 cgtgcgccgc gccttcactg caccgccggg cagcgtcatc gtttccgccg actattccca
     7861 aatcgagctg cgcattatgg cgcacctctc cggtgacaaa accctcattg ccgcatttca
     7921 aagcggcgaa gacgtacacc gccgcaccgc cgccgaagtg ttcggcactg cgcccgaaaa
     7981 cgtctcgccc gagcaacgcc gctacgccaa aaccatcaac ttcggcttaa tctacggtat
     8041 ggggcaatac ggtttggcaa aatcattggg catcgacaac ctttccgcca aaaactttat
     8101 cgaccgctac ttcgcccgtt atcccggcgt cgccgaatac atgcagcgca ccaaagaaca
     8161 agccgccgcc caaggctacg tcgaaaccct gttcggcaga aggctctatt tgcccgacat
     8221 ccgcaacaaa aacgccaacg cccgcgcagg agccgagcgt gccgccatca acgcccccat
     8281 gcaaggcacc gcttccgacc tcatcaaacg cgccatgata aacgtgcgaa actggctttc
     8341 agacggcatc ggcagcaaac tggtcatgca ggtgcatgac gaactggtgc tggaagtcgt
     8401 tgaaaccgaa ctggattttg tcaaagaaaa actgccgcag attatggcga aagtggacgg
     8461 cggattattg gatgtaccgc tggtggctga ggttggcgta ggggagaatt gggaagaggc
     8521 acattgagaa tgcacaagta ttgaactgtt acgttttgat ttatcaaaat ttaaggaagt
     8581 aaaatacccg attcgaatga ttggaaatca atagcaagcc gtaggtcgga tacttgtatc
     8641 cgacaaaagc ctgccatctc aaatagcagt cggattcgag aatccgacct gccaaaccgg
     8701 gcgcggacgc tccggtcggc agttagcgca caaatcgaac agaacatcac aaaaaagcct
     8761 gattcggatt ttccaatcgg gcttttttgc gcccgttttg tcatcccgtg aaatatccgc
     8821 ataacaaaaa taatgcaaaa tcatgacaaa accggcgcga ggttacacaa acggatgaaa
     8881 tcaaccgata ttcaaacaca gccattttta gcgcattttc agcgtatctt tatgcggaaa
     8941 atttcgtgaa caggtttttt gcacaggctt ccgccgcgtt tcgcgggatg ggaaaccgta
     9001 ttagaaaacg gacgcacgca acataaaccc cgaaagtgat gataagatga tgatttaacg
     9061 tactgcttta attatttaag gaattatcgt gtttcccgac aaatacaagt tgagtttgga
     9121 agaaaatatt tttctggcaa agaaagtatt ggttgctcaa attcacaacc tcagccgttt
     9181 tgagaattgt cagacgacct tgttgcagac cgagcaaatt atcaatgaca aaaatgtagc
     9241 ctccgcgtca ctggaagaca tccaaaccat cttgaacctg aaacgtgcct atcaatatgt
     9301 gatttcgcat atttcaaacg gcgaaccggt caatatttca ctccttaaaa gaatcaacaa
     9361 cattgttgcc aaggacgatt ctttggtacc cgtggatttc cgtaccggtt cggtcggcgt
     9421 aacgctattg gacggttccc gtcatgcccc gaatccggtg aaggaaattg aagtggcccg
     9481 cgtgttgcaa aatatcggac tgcaaagcgg ttcgacgacg gaagcagccg ttcgtttcat
     9541 gctctattgt atgcggcagc aggttttttg ggacggcaac aaacggacgg caaccctatt
     9601 tgccaacggc ctgatgatgg cggggggctg cggtatcctg gaaatctccg aaatgcagat
     9661 gccgcgattc aatgaaaaac tatccgcatt ctatcgcacc ggcgacgata ccgatatttc
     9721 caagtttgtg tatcaaaatt gtatatcggg catagactat ttcggcgcgg acgaagatat
     9781 atagtggatt aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttttactg
     9841 tctgcggctt cgccgccttg tcctgattta aatttaatcc actatagatt tccccgactc
     9901 gccgaacatg ggctaaaaat caatttgacg gttatcagac gatggagtag gcacaaggtg
     9961 gcagaaaaag gggtttgaca gtgcacggcg gcatcgtcaa acccctttcg gcatatccgg
    10021 cggttaccag cggtagccta atttgatgcc cgcgctgtgc tgcgcttcca attgcggccc
    10081 cttggcggcg gcagcgtgga gggacagcgt gaaacctttg atttcggcgt ttacgcccca
    10141 ttccgcactg cgggttttgc cgaaatcctg cgccaatacg gcggtattga cgcgcgttcg
    10201 gactttgccg gaagcggcat cggtatagga caggctcaaa taaggcgtga tggaaatgtg
    10261 ttgcgccggt ttgaatgaat aatctgcctt aatgcccgcg cggtagcggt tgaatgcaag
    10321 gcccggggtg gcgatattga cgttttcgta tcggtaatcc gctttttgga cgaaatagcg
    10381 cgttgcgccg atgtgcggtt cgatgccgaa tccgccgaaa cctgcgcggt atcttgcctg
    10441 aatgccgtaa tgcagcacgc ggcggcggat tttgcctctg atgccgtctg aaaggctgcc
    10501 gctactaaaa cccgcgcccg cgctgatgcc gatgtcgaac ctgccgatgc cgtattgccc
    10561 gaaaacggca ccgtgggcaa gccgtgccga gttgccgatg ccgtcgtcga aggtgtttcc
    10621 ggtccggttg tgcgaaaaca ggatgccgac gcgcccgctg ccgaggtttt tctgcatacc
    10681 gatttggcgc aggtcggttt gttggcggta ggcgcggaaa tcttgcgaac ggtagtgttt
    10741 ggtgtcccgg atgccgcttg tccaaacggc gttgcggcgg tcttcggcaa acacgcggtc
    10801 caattcgtcc tgtacggcga aaacgctgtt gagcgtggcg gaaaattcac tcaaaccgct
    10861 attggcataa cggctgatca ggtcgcgctg cggttggggt tgcggttggg gctgcggttg
    10921 cggcaaatcc cggcgggcgc ggcgggcgcg ggggaaggcg gtggtagccg gccgggtttc
    10981 cgcttcgcgc tgtttcgcca aggcggtgtc tttatccgcc tgcacccgtt ttttctcttc
    11041 ctccgcctgc ataatgccgg cattttcccc gcctgcctgc cgggccggtt cggcaacgct
    11101 ttctgccttt tcggtggcat cgcgcccggc cgcaatcagc gcgtcaaggc tttgcgcgtt
    11161 gtctttttcc gcctgttgtt tggcggcaag ttgtgcctgt tttgccgtca aggcggcctc
    11221 tgtttctccc gccttgccga gtttgtcgga aagctcttgt tctttgaccg gattatgcag
    11281 gcggaactcg ccgtctttgc ggataagctg ataacgccat gcgccggcat cgacgtgttc
    11341 gttttgcagg gtgaaattaa gattttcgga cagcggtgtg ttgtcttttc cttccactac
    11401 cgtcaattgc tcgagactta cgggttcgtt gccggtattg ttgacagcca aggtgtaagt
    11461 gccttcggaa ctttccgcca gcttcaattt gccgctgcgg tagccgaaga gttccgacat
    11521 aaagcggaat gttccctgac cgttcaattt gccgtttacc gtcagcgtgt tgaaacggga
    11581 ttctgccgaa gttggcggcg taacggataa tagggaacgg cgcgaacggc ggcgcggcgc
    11641 atctgccgca ctgccggttt gcgcgcctgc cgcatcgtgt cgataggcgg aattgagtgt
    11701 aatggtggcg ttgtcaaggt ttaaattgcc taattccgtg cccgacggca gcgtccattc
    11761 gctgtctttt aagtgtaatg ccgtatcctt gccgccgctg atttttccgg taaagcggct
    11821 gttttcaaaa tggaatactg ccttatcggc tagggagaca ttgccgttga gtgcggaatg
    11881 gcttacgttt gccttagcgt tgtcggaaag cgtcagactg ccgttttgta cggcgttgtt
    11941 gcttagatta aatgaagcat tgtccgaagc cgatgtgttg ccgtttaatg tggcttgatt
    12001 aaatgttgct tgggcattgc ccacgaggct aaggttgccg ttttgggtgg cgttgcgcgt
    12061 aaccgtatag tgcgtgtctc cgcctgcact aagattgccg ttgagtgtgg caagtcctgt
    12121 gagatttaaa tgagcgtgat cggcaaggct gacattgcct ctgatgtcgg tcttgctcaa
    12181 tgaagcaatc actttatcgt cggtaatggt tttttcggta caacttgtca gacccgtcca
    12241 gtccgaacgt gtacagattg tgtggctttg atgcggtgcg acaccgaaaa ctgcttgggc
    12301 gtgattgctt aaatgccaat cgccttccac tttggcaaca ttgcgggaaa ccaccgcttg
    12361 tccgccctga atatggaagt tttccgcttt aaatgtgcgg tcgatccaat cgttgtccca
    12421 cacgatttct ccttgtggga taccttccat ttttgaccac ccgcttccta aatgattgta
    12481 ggcgtgcggt gtcggtctgc cgctgaaaaa cagtttgccg tttgtttgcg tgatattgcc
    12541 gtttaaattt gttccgccgg aaagcagtaa agtgcgatcc gcttcttccg gttggtaatt
    12601 cagattgagc cgcccgttcg ttttggttgc atctttctcg ccaaaccaac cgttgtaggc
    12661 aatttctttt ttgctatcca agttgttgtt attgccggtt gtagtaatat ctttattgcc
    12721 tgtaatggta acggtggatt ctttgtcttg attgtggttg acaatcatcg ccccttcatc
    12781 ggtattttga atgcggtgga acgaaagcga atgcccgttc aaatccaaac gtccgccgcg
    12841 aaagccgaaa tagagtttgt cggggttgaa ctgattatcg gcattcagtt gcaccgtccc
    12901 cctgccgctg accaagccga tttcactaaa ggcttgtttt ttgccttgat cgtccgcctg
    12961 ctgatctaag atgactttac cgtcgcccac gctgaccgag ccttggtttt cccctttggc
    13021 ttgaaccagc agcgtgcctt tgccgatttt ggacaggcgg tcgtttgcca cgccgtttac
    13081 tttccaagta acggtactgc catcactgat atgaacgccc gcgccttgcc acgtttcgtt
    13141 gtttttaggc gagaccgtaa aattaccctc aaaatacaaa ccgcccgcgc cttggttgat
    13201 gttgctggta agtatcaatt caccttttcc tttgtcaata aaggaaatat tttctccatt
    13261 attcagtctg ggtcgataac tgttgacccc acctgcagca tgataaacag gttctcttgc
    13321 tgtctcggat aaagaaacat taaacaattg aacggttcgt gtttttaatc tataaggtag
    13381 agaatagtgt ttatgtttgg catcgatttt tcctgcgcca ttattattgt cgttaaaaaa
    13441 gtatttccca ttttgatgtg gttcgtagaa tactgaatgg gtatctccag caaagatttc
    13501 atcatagaac caatctttac gaactagctg gaagccattg ctttttccta tataggggtt
    13561 gcctgtttgc aataccccat taattaacca cttttgcttt tgggcatcat agataaacat
    13621 tggtgagcca ctgtcgccaa atgagcctcc tgttggtaaa aaaccatatg ggctatgttt
    13681 aattttttcg ctacctaagt tgactgtgcc accacctgat ccattttgtg caaaggtatt
    13741 gccaccgacg agccaagaat atgcgcttgc aatatgatat gaactttcgc ggttattggg
    13801 ttcgtcttca tcagaccgcc aatattgtct gcctgctccg attcgaacac gatcagggta
    13861 tttatttaaa tcagcgtatt tccacccatc catataactg gtcatctcaa caggttctgc
    13921 atctgtgaca aatttgtgca aacgcggcat atgataatcg ccgccataag gatggccgtt
    13981 agtccctgct ttataattat ttcttttcac aatttggtaa gaaaagcggt gctgatcggg
    14041 attgcttccc tccgcaccaa aatcaacatt gttatagccg ccgttatgtg ccacgctcac
    14101 aatatattga tcgcccgcca atgccgccac gccgttacgc gataccacag aaaaatcaat
    14161 catcggggct ttcgtcatcg atttgccgac caactcccct tttttgttgt aaacctcaat
    14221 atctttcgcc ccgactgcaa acttgccttt attttcggca aagtcgcgat agtattggta
    14281 gttgatgccg aaataagtgt gtcccgcccg ggcttggggc agaatgccga acgacaggca
    14341 tatggctaag taagcgggcg agaagcggat gcggccggtt ttaggggctt tgcggtgtgt
    14401 ttcggttgtc cgtttgtcgg ttgttttcat tatttttcct tatctgacgg gattcggttt
    14461 gtttgggagg gcgcggcttc cgcttccggg cggcgcgcgg gatgtggcta tatgtgcggt
    14521 tcggcgtatt ttgccgcttc tgttcggaga tggtgcaggg aagggcggga aggaaaaaaa
    14581 ggggattttt atcaactcgc cggagcaatc gatgggttgc acataaaggc atcattttga
    14641 atacgaaatc atatcacact ttactttatc cccgttcctt ttcggtcgga accccgccgt
    14701 tttcggcggt aaaaaccgac cggaggcgga atgcggggca gggttttgtg ccgtgcggtg
    14761 tcggaatatc gttttacaca agccggcaat gccatctgaa agccgaaccg tcggacttca
    14821 gacggcattt gctataatcg cggctgtttt gaattttcgg gggttttatg tcggataacg
    14881 ttccaacgat tgcggcagtc gcgaccgcgc ccggacgcgg cggcgtgggc gtgatacgca
    14941 tatcggggaa aaacctgttg ccgatggcgc aggctttgtg tgggaaaacg cccgagccgc
    15001 gcgtggcaac ctatgctgat tttacggacg cggacggaca ggcaatcgat agcgggctgc
    15061 tactgttttt tgccgcgccg gcaagtttta cgggcgaaga tgtcatcgag cttcagggac
    15121 acggcgggcc ggtagtgatg gagatgctgc tgaaccgctg tttggaactg ggcgcgcgcc
    15181 ttgccgagcc gggcgagttt accaaacgtg cgtttttaaa cgacaaactg gacttggcac
    15241 aggcggaagg cgtggcggat ttgattgacg catccggccg ttcggcggcg cgtctggcgt
    15301 tgcgctcgct caagggcgat ttttcgcggc ggatacacgg tttggtcgaa gacttgatta
    15361 ccttgcggat gctggtcgaa gcggcgctgg attttcccga ggaagacatc gattttctcg
    15421 aagcggcgga cgcacgcggc aaactggacg gcttgcgccg cgccgtggat gatgtgcttg
    15481 ccaacgcgca gcagggcgcg attttacgcg aaggtctgaa tgtcgtattg gtcggtgcgc
    15541 cgaatgtcgg caagtccagc ctgttgaacg cgctggcggg cgacgaagtg gcgattgtta
    15601 ccgatattgc cggaacgacg cgcgacgcgg tcagggaacg tatcctgatt gacggcgtgc
    15661 cggtgcatat tgtcgatacg gcaggtttgc gcgagacgga cgacgtggtc gaacgcatcg
    15721 gcatcgaacg cagccgcaaa gccgtatccg aagccgatgt cgcgctggtg ttggtcgatc
    15781 cgcgcgaggg tttgaatgaa aagacgcgga tgattttgga tacgttgccg tcggatttga
    15841 aacgcattga aatccacagc aaatccgatt tgcacgcaca cgcggcaggc gggttcggta
    15901 cgggcgcgga aaccgtcatc gcgctgtcgg cgaaaaccgg cgacggcttg gacgcgctga
    15961 aacggacgct gttgtgcgag gccggttggc agggcgaaag cgaagggctg tttttggcgc
    16021 ggacgcggca cgtcaacgca ctcaaggcgg cgcaggaaga attgtcgctg gccgctttgt
    16081 gcggcaacca tcaaatcgag ctgtttgccg aacacttgcg gctggctcag gtcgcgtgcg
    16141 gcgaaattac gggggaattt acggcggacg acctgctcgg cgtgattttt tcgaggtttt
    16201 gcatcggaaa ataagcgggc cggaggcatt gtggcggtgt ccggctgaac attccgttat
    16261 cccataaaaa cgggaatccg atccgttcgg ttttggtttt ttgaatttcg ggcaacgctt
    16321 aaatcttcat tccgcgcagg cggaaattat cggcgcggta cggcaacttt tttcaatact
    16381 gaaagccccg tcatttctgt aaaaacaaaa aaatcaaaaa cagaaaactg aaatatcgtc
    16441 attcccgcgc aggcgggaat ccagccccgt cggcacggaa acttatcggg aaaacggttt
    16501 cttcagattt tacgtcctag attccc
//
LOCUS       DAXFHF010000052        16294 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXFHF010000052 DAXFHF010000000
VERSION     DAXFHF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.052"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            291..737
                     /gene="yciA"
                     /locus_tag="ACFIRM_001549"
     CDS             291..737
                     /gene="yciA"
                     /locus_tag="ACFIRM_001549"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC3029145.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(788..1609)
                     /locus_tag="ACFIRM_001550"
     CDS             complement(788..1609)
                     /locus_tag="ACFIRM_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3029146.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1728..2186
                     /locus_tag="ACFIRM_001551"
     CDS             1728..2186
                     /locus_tag="ACFIRM_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3029147.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2329..3465)
                     /gene="lst"
                     /locus_tag="ACFIRM_001552"
     CDS             complement(2329..3465)
                     /gene="lst"
                     /locus_tag="ACFIRM_001552"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC3029148.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            3602..3742
                     /locus_tag="ACFIRM_001553"
     CDS             3602..3742
                     /locus_tag="ACFIRM_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC3029149.1"
                     /translation="MPPHIRHNAADAAAKRGKAISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3809..6034
                     /locus_tag="ACFIRM_001554"
     CDS             3809..6034
                     /locus_tag="ACFIRM_001554"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC3029150.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEITAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAATLTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6331..6633
                     /locus_tag="ACFIRM_001555"
     CDS             6331..6633
                     /locus_tag="ACFIRM_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029151.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
     gene            7283..7438
                     /locus_tag="ACFIRM_001556"
     CDS             7283..7438
                     /locus_tag="ACFIRM_001556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029152.1"
                     /translation="MPSETIFRRHFSLILLGEWLTLNVSTFIIQFIGASGADTGKAPP
                     KRGLYRE"
     gene            7593..7760
                     /locus_tag="ACFIRM_001557"
     CDS             7593..7760
                     /locus_tag="ACFIRM_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029153.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            7920..8765
                     /locus_tag="ACFIRM_001558"
     CDS             7920..8765
                     /locus_tag="ACFIRM_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3029154.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTTSGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGCQVGPKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESIEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTWMIESGKD
                     ISAPLKQLGCRQAVTMWTVWHETRPILKRSAAALEVLRGYADKDASDRIAACLEGIYG
                     KATVR"
     gene            complement(8802..8963)
                     /locus_tag="ACFIRM_001559"
     CDS             complement(8802..8963)
                     /locus_tag="ACFIRM_001559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029155.1"
                     /translation="MDLTMSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADY
                     REMLKRYAA"
     gene            complement(8954..9628)
                     /locus_tag="ACFIRM_001560"
     CDS             complement(8954..9628)
                     /locus_tag="ACFIRM_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029156.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(9625..10110)
                     /locus_tag="ACFIRM_001561"
     CDS             complement(9625..10110)
                     /locus_tag="ACFIRM_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029157.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(10116..10589)
                     /locus_tag="ACFIRM_001562"
     CDS             complement(10116..10589)
                     /locus_tag="ACFIRM_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029158.1"
                     /translation="MARIHVRNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADAYG
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAHVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(10646..11941)
                     /locus_tag="ACFIRM_001563"
     CDS             complement(10646..11941)
                     /locus_tag="ACFIRM_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC3029159.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVAAKGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(12567..>16294)
                     /locus_tag="ACFIRM_001564"
     CDS             complement(12567..>16294)
                     /locus_tag="ACFIRM_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC3029160.1"
                     /translation="RTGKITLVAENLTPERAVWAAWHELGHRGFAADGFAKYREELER
                     ADGNGLIRRIADAVQEGREGTGDAAASVRSAAVEEAVAELYAAQRTGGWAGIENRYGV
                     KVGNGLKRGIAGVLARIGALLRRVLQRLAGKAGGAMSDADVFAMLADLHGNVEGARDA
                     PWGGNHRAVMFARAEDGAAERSKSESLEKLRRAETIRISGREVPEGGNLREYKRNALE
                     YGKSLRGPYVNKDTGREISLGRSGITEILRHDYKDAEHLQSIAAIPQIIENAVYIDTL
                     PNEDLAKNGDIQGYEYYVSGLNVGGADYTVRAAVAVSRNGNRYYDHKLTKIEKGNLLS
                     LLDRVSTTGASESKSPLSGIDDKRLLQILQDKDAGKGGIADFDTEAVRFSRAANIGAA
                     ISRITGKKSDLRNALKDRWDASKGIQLQFLGRRQIEDIYGGVLDGLKEYGRLSELFGA
                     DANKAVTEADKVVREWGRLKEEDAKALADLMHDATLAKVDADPLMRKDARGRLDGIRT
                     ALDIADGKIAKARAAVASAGARTARADAAYNKAQRAADKAAYALEKAQEKHGREILAD
                     EADMRLRRLFYADSEAKRALRRAGADAAAESRAKTDAVRMLEQARADVKRLEKDEVGA
                     QKALEGLALLNRRFAGLPDAAQRVYRKARDDYRAHFGQVRDALAERLARAGQDAETVR
                     RLKERFDNELGGVYFPLARFGDYLVVVKDADGNSANVSRAETLSEAEKLRDALKADFG
                     PGFKVSPVMKSRDYIRSRDAVGSGFMRELGEAVGMLDLDPAQRARLNDTLTQLYLNSL
                     PDTSWAKHGIHRKGVPGFSDDARRAYAQNMGSGANYLAKLRYADRMAEQLDVMQDFVD
                     GRKYEEGFDQRQLQRVADEMRKRHEAVMNPNPSKLAQALTGFGFLWMMGMSPASAVVN
                     LSQTAVVAYPVMAAKWGYAGAARELLRASKQIGLRFGEKFNTIEDSLNGDEKAAFRKA
                     ADYGVIDLSQAHDLAGVANGDPGLAGSAWQKVMDKAAWLFHHAEKFNRQVTFVAAYRL
                     AKRAGADSDAAFEQAKKATYDGHFDYAAQNRPRFMMGNAAKVVFLFKQYSQNILYALG
                     RNAYLAFKGDKEARKTLAGLLVSHAMASGILGLPFVSTLLAVASMLGSDDDDPWDAEA
                     ALRNMLADTFGDKAGEVLAKGFSRLTPLDVSGRLGLDQLVFPDIQDGLEGKKWAESLV
                     VGSTGAVVGAGIGAADGVRTRSSVPRTANTLSLMKSW"
BASE COUNT         3980 a         5221 c         3691 g         3402 t
ORIGIN      
        1 atgcggaatc tagagaaacc ttttaaccga taagtttccg tgcggacagg tctggattcc
       61 cgcctgcgcg ggaatgacgg ttcagttgct tagggtagga tttggcggga ttggtgggct
      121 gaagcccacc ctacggcccg ccctacgcat ccgccctaca atactccttg cgcatccgtc
      181 cctcgcccgc ccttgtatgt ctcaaatggg ttaaactacg cacttcccga cttcccgttt
      241 cgggcagggg tgcaggtccg atttatattt caataaaaca aggaaacttt atgcagcacg
      301 aagaaggcaa ccgccaacgc cctcaaggcg aacttctcct gcgtaccgtc gctatgccgc
      361 gcgataccaa tcccaaccaa gacattttcg gcggctggat tatgtcgcaa atggatttgg
      421 gcggcggcat attggcggcg gaaatcgcgc gggggcgcat cgttaccgtc gccgttcagg
      481 aaatgaactt catccgcccg gtcaaggtcg gcaacgtcgt ctgctgctac gggcattgcg
      541 tccgcgtggg caatacttcc ctccagctta aagtcgaagt ctgggtgaaa actttgatga
      601 acgattgcgt taccgaagac cgccatctcg taaccgaggc ggtgttcact tatgtcgcca
      661 tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg caaccccaaa ttgtcgggct
      721 tattgcccac cccgtaaaaa tacccgtaaa aatgccgtct gaaactgtct tcagacggca
      781 ttccgtatca tttgaaccaa gacctgatgc gggaaaacag cgacatactc tgtttttcaa
      841 agtcttcccg cacttcgggc acggctgttt ttatgacctt cattcctgca aaagtctgcg
      901 ccacaggcat aatttcaatc gtgttcacat tcatatgcgc cggccggcgg tacagccaca
      961 atgcggtttc cgcaatatct tcggggcgga taaattccac accctcatac acgcccgcca
     1021 ccctctcgtt atcgcctttg aagcgcacgt tggaaaactc cgtattgccg cacaaacccg
     1081 gctcgatatt ggtaacgcgg atgttcttat ccgccagctc tgcgcgcaaa ttcaggctaa
     1141 actggcgcac aaacgccttg gtcgccccgt aaacgttgct gccggcataa gcataattgc
     1201 ctgcaatcga acccaaattc atcacataac cgccgccgcg ttccaccatt tgcggcaaaa
     1261 tcttgcgcgt caggaacgtc aaacccaaaa cattggtttg aatcattgtt tcccaatctt
     1321 caaaatccgc cttgtccgcc gtgtccaaac ccaaagccag cccggcattg ttgatgaggc
     1381 agtcgatgtc ggaaaattca tcggggatgc cgttcaaggc gttttccacc gactcgcggc
     1441 acgacacgtc catttccaaa gggtaaaaca aagcacccaa ttcatccgcc aaagcctgaa
     1501 gcctgtccgc acggcgcgcc gcaccgataa cgcggtatcc cgccccgaca aacgtgcggc
     1561 acatcgcttc gccgaagcct gccgaagcac cggtaattaa aacagccatt gtttttcctt
     1621 tctttctcgt ttcccaaacc tgtttcaggt atcataacac ccttcagacg gcatcgcgcc
     1681 ggctgaaaac gacaacaacc gtaccaccac gacaggacac attcaacatg aaaacccaaa
     1741 tcagccttgc cgcaaccgcc ctcgccctat tcctttccgc ctgcggaaac ggcggcgcgc
     1801 ccgcacagcc caaaggcgaa atttccgaaa accgcaccgc cgcgttcaaa tctatgatgc
     1861 ccgacttctc gcgtatgggc aaaatggtca aaggcgaaga gccttacgat gtcgaaaaat
     1921 tcaaacaggc ggcggcggcg tttgccgaaa gcagcaaaaa accgttcaca ctttttgagt
     1981 ccgatccgca aggcaacggc cgcgccctgc ctgccgtttg gtcggatggt gcaaaatttg
     2041 aagccgaaaa aacaaaattc gccgccgccg tcgaaaaact caacgccgcc gcccaaaccg
     2101 gcaaactgga cgaaatcaaa gccgcctacg gcgaaaccgg cgcaagctgc aaatcctgcc
     2161 acgacagttt ccgcgcgccc gaataacatc cgaaacagaa caatgccgtc tgaatccttc
     2221 agacggcatt ttctttgtca tttcgccacg cgcaaagcaa tcggaaatta tcttcaattc
     2281 aaaaaatacg ccatatcctg atttttgcct tacttactcc gccattttct attttgattt
     2341 accatgcgat tgatttttat cgtcaaatgc caaaatcgga atgtcggcct gacggaacag
     2401 cgcataaacg ggcttgagcc aataatcttc cggaagggaa gccggtttca atgcgtaaac
     2461 gtgcacattg ggaaaatcct tcatcgtcaa cgccgcaccg ctgaaaaagg tataaatttc
     2521 atacctcgta tgcgggtttt tcttaatttc gcgcaaaata tagtcttcga tgacataggg
     2581 cgaatttaac gcggttacgc cggaaagccc gtaggtctgg cggggatgcg gcgcgacata
     2641 ttgtatgttg aaattttttg ccgccttttc cgaaatttcc ttcatctctt tgtcgggcga
     2701 acccaaaagt atccgcaccg tgccgcccgt ttcgtccccc gccttcagtt cggacgcatc
     2761 gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc ataatgtttt tcaaaccctt
     2821 gaatatcgtg taatgctcgt cggaagcatt gcgggttttg gcgatgctcc aatccccgac
     2881 catcattcgg gcaaaattcc gcttaatcgc cccgtttaca taaaactcat cgcccaaata
     2941 gctgctctcc cgtatcaggt tgtttgtgcc gtcgtcaaag gtcttgattt ccgcatccgg
     3001 gtaagtgctc aaaaaggcgg cgatgctgac tttttccaaa ctcgccaaat aaatccgcct
     3061 gaccttcggc agcagcatcg acttcacctt cagctccgcc atcgtcggaa tgaaattaaa
     3121 cgatttgttc aaaccgtagg gcaggtagaa aaaatacgcc cgctccgcct tatcctttat
     3181 ctgattgaaa taataatcgt atttttcatt cctgttttca gacatcagca ccacataaaa
     3241 ccgctccccc ggatgctgcg ccataatcct ttctgccacc ttcatctgca atatggtata
     3301 gcagaaaatc agattgacgg gtttcccctc ttcattgaag agtttgtcct tcagcaggga
     3361 aaccgcgttc ctttccccct gatttacccg gtcaaacgta taaaatatcc cgaagcaaaa
     3421 aacaatcagg cacaacacgg tcaaacagac ttttttcaac cccatatccc taaaaactcc
     3481 attccgacaa attgaacata cgccccgagt gtcccggtgc ggaaactccg gtgcggaaaa
     3541 tgcggaattt tagcagatat tccgacagtt gccgaatatt aaaattaatt ttaccgaaaa
     3601 tgtgccgccg cacatcaggc acaacgccgc cgacgccgcc gccaaacgcg ggaaagccat
     3661 ttcccccttc ctttcccgcc gccgcgttca aacacgccaa gatcgaacaa agcgcgaaca
     3721 aagccgccaa aatatgccat aatagccgcg cacattcccc gcgcaatgtg tttcaaaaga
     3781 aaaaaccaac ctacaagaga ataatcctat gactcaaaaa tccaccattg tttataccca
     3841 taccgacgaa gcccccgcgc tggcgaccca atcgctgctg ccgattgtgc aggcgtttgc
     3901 ccgccacgcc gatattgatg tcaaaactgc cgacatttcc ctgtccggcc gcattttggc
     3961 ggcattcccc gaatatctga ccgaagcgca gcgcgtaccc gacgcgcttg ccgaattggg
     4021 cgaactggtg aaacaacccg gtgcaaacgt aatcaaactg ccgaacatca gcgcatccgt
     4081 acctcaactg accgccgcga ttaaagaatt gcagtctaaa ggctttgccg ttcccgacta
     4141 tcctgccgac cctcaaaccg atgaagaaaa agccgtacgc gaacgctacg accgcatcaa
     4201 aggcagcgcg gtaaaccctg tcctgcgcga aggcaactcc gaccgccgcg cgcctaaagc
     4261 ggtgaaaaac tttgccaaaa aaaatccgca cagcatgggc gcgtgggcga aagactccaa
     4321 aacccacgtt gccaccatgc aaagcggcga ctttttccat aacgaacaat ccgttaccgt
     4381 acccgatgcg acttccgtat ccatcgtgtt caccgacaaa caaggcaata aaaaagagct
     4441 gcgcgagcct gttgccctga aagccggcga aatcattgac gcggccgtaa tgagcaaaaa
     4501 agccctgctc gccttccttg ccgaacaagt gaaagacgca aaagctaaag gcgtattgtt
     4561 ctcgctgcat atgaaagcca cgatgatgaa agtgtccgac ccgattatct tcggacacgc
     4621 cgtcaaagtg ttcttcgcgc ctgtatttga aaaattcggc ggcaaactgg ctgccgcagg
     4681 cgtcaacgtc aacaacggct tcggcaacct gattgccaat ctggacaaac tggatgcgga
     4741 cacccgcgcc gccgtcgaag ctgaaatcac cgccgtttac gccgccaacc cggatttggc
     4801 gatggtcgat tccgacaaag gcatcaccaa cctgcacgtt cccagcgatg tcatcgttga
     4861 tgcttctatg cctgcgatga tccgcaattc cggccgtatg tgggacaaaa acggcaaagc
     4921 gcaagacacc aaagccgtga ttcccgacag cagctatgcc ggtgtttacc aagcaaccat
     4981 cgatttctgc cgcgaacacg gcgcattcga tccgacaaca atgggtactg tgcccaacgt
     5041 cggactgatg gcgcaagcgg cggaagaata cggctcacac aacaaaacct ttgaaatcga
     5101 agccgacggt caggttcaag tgattgatgc ggcgggcaat gtcctgatgc agcacgacgt
     5161 tgaggcaggc ggcatctggc gcatgtgcca aaccaaagac gctcccgtca aagactgggt
     5221 acaactcgcc gtcaaccgcg cccgcctgag caacacgccc gccgtgttct ggctcgacga
     5281 aaaccgtccg cacgacaaga gtctgctcgc caaagtcaaa gcctaccttg ccgaattgga
     5341 taccaacggc ctcgacatcc gcgtcctcgc gcccgaagaa gccgctaaat tcagcttggg
     5401 ccgtctgaaa aacggcgaag acaccatttc cgtaaccggc aacgtcctgc gcgattatct
     5461 gaccgacttg ttcccgattt tggaactggg taccagcgcg aaaatgctgt ccatcgttcc
     5521 attgatgaac ggcggcggta tgtttgaaac cggcgcgggc ggttctgcac cgaaacacgt
     5581 ccaacagttc ctcgaagaaa accacctgcg ttgggactca ttgggcgaat tcctcgcgct
     5641 tgccgtatcg tttgaacatc tggcgcaaaa aaccggcaac gccaaagccc aagtcctcgc
     5701 cgacactttg gatgccgcca ccgaaaaact gctgttgaac gacaaatcgc ccaaacgcaa
     5761 agcgggcgaa ctcgacaacc gcggcagcca tttctacctc accctctact gggcgcaaga
     5821 attggcggcg caagacaaag atgccgaact gaaagccgca tttgctccat tggcagccac
     5881 tttgactgcc gacgaagcca aaatcgttga agaactctct gccgtacaag gcaaagcggt
     5941 cgacatcggc ggctactacg cagccaatcc tgaaaaagcc gcacaagcga tgcgtccgag
     6001 cgcaaccttt aatcaagtac taaacgcctt ataagcacaa aggcaaaaat gccgtctgaa
     6061 cattcgtcgt tcagacggca ttagttatcc ctatctgcct gattatgatt aaacgccgca
     6121 cccattggat atatagtgga ttaacaaaaa ccagtacggc gttgccccgc cttagctcaa
     6181 agagaacgat tctctaaggt gctgaagcac caagcgaacc ggttccgtac tatttgtact
     6241 gtctgcggct tcgtcacctt gtcctgattt ttgttaatcc actataaatc atataatctt
     6301 tagtttttta ccctacggaa agacaataat atgaaaactg ttccaaccat cctgaccggc
     6361 atccttttgg caaccgccct tcccgcatcc gcacacggta tgcataagag caaacccctg
     6421 gctatggacg agctgccgcc gatttgccaa caatatttca aacgcaccga aacctgttac
     6481 aacaaagccg gaaacaaagc agatttcgca cgcaacaaca ccaaattcct attccaagcc
     6541 ctacctgccg ccgatttggg acagcgtaag caaatgtgcc aaatcgctat ggattcgttt
     6601 gcagaaaaaa cccgcaatct gaattgcgaa taaagccgca tcagcaatag caatgccgtc
     6661 tgaaacaaag gttcagacgg cattctcaca tcaaaacgta ccggctgata aaacccgcct
     6721 tccccgcatc cctgacccga acaggattaa acggagtaac ggtttcggcc attatggcaa
     6781 gtccccatcc caaaaatgac catataaatt aataccataa aacattgata aaaagcgtct
     6841 gccggttgcg gtttgttgca gggcgttacg gaaaatcatc gtaaaccgat gatttttata
     6901 tagtaattga cacaacctga acaaaaaaga accgccctga atcagggcgg ttttgctttg
     6961 cggcggaaac ggcaggattc gactaaattt tattttattg atttaaatac atttatttct
     7021 tataaaaatt taatttacca taaaaacagc catatacaaa aatcttggag taactactgc
     7081 attacactat tagaaggatg cggggtcatg taatatttaa ttaaattaat atataaatta
     7141 aatatttgaa ttatatttat aaaaaaaggt gcaaataagg tgcaaataaa attagttttt
     7201 cccaatgggt taaagaacaa atccgaaaag agttcttaaa atagcccgac cgccgccaag
     7261 ccgcaaactt ggcggcttct ttatgccgtc tgaaaccatc ttcagacggc atttttcttt
     7321 gattttgttg ggggaatggt tgactttgaa cgtgtccacg tttataattc aattcatcgg
     7381 cgcatcgggt gccgatacgg gaaaagcccc accgaagcgg ggcttgtaca gggagtagga
     7441 aaatgaagct catccttcaa attctcatcc tggttttaat cttgatgctg gcaggcgata
     7501 cgcctattag cggataaaac cggctggggc ggtagccgcc gccccagtcc ctatcttaca
     7561 aaaggcagtt caaaaaatca aggagtttgc ctatggttaa tgaaaaactg gcagaaaacc
     7621 gaaagcgtta cgagcaaaaa cgagtcatta aaaaagtctc gtttaatgcg gaaacagaaa
     7681 aagagctttt ggaatatgcc caaaatctcg atttttccca atgggttaaa tcaatcataa
     7741 aagaaaaaat caaaaagtaa ttggctttga acgtgtccac gtttattatt cttacaacgg
     7801 cacagccgtt aatggaaaac cgaaccaagc gggaacttgg ttcggttaag tgaaaagcag
     7861 ttctaggaaa actcttttcg cgtcttttaa ataacctcta aaagaaagac tattctatca
     7921 tgaatgctgt ccaagtttta aactttcaac aaaactccgt ccgtactgtt gccgataaca
     7981 aaggcgagtt gtggtttttg gcaaacgatg tttgcgagat tttaggctac accaaccctc
     8041 gccgaacggt tgacctgcac tgcaaaagca ggggcgtaac aaaacggtac acccccacca
     8101 ccagcggcga acaggagatg acctatatta acgagccgaa tctctaccgc ttgattatca
     8161 aatcccgcaa gcctgccgca gaagcgtttg aagaatgggt aatggaaacc gtcctgcccg
     8221 ccatccgcaa aacaggcggc tgccaagtcg gaccaaaaac caccgccgac gaccgtaccg
     8281 gattgcgcca agctgttgcc gcgcttgtcg gacgcaaagg tatagactac tcctccgcgt
     8341 acagtatgat acaccaacgc ttcaacgtcg aatccatcga agacctccct gccgggaagc
     8401 tgcccgaagc cgtcgcctac gtccacgcat tgacactgca cacgggcttg acaggcgaag
     8461 tgttggacgc accgcccaaa gccgagccga aactgcccat cgacggcaac tctttagccg
     8521 acattgccgc tatggtttat tacggcacat ggatgattga atcgggcaaa gacatctccg
     8581 cgccgctgaa gcagctcggc tgcaggcagg cggttacgat gtggacggtt tggcacgaaa
     8641 cccgtccaat cctgaaaaga tccgccgcgg ccctcgaagt gttgcgggga tatgcggaca
     8701 aagacgcctc cgaccgcata gccgcatgtc ttgaaggcat ttacggcaag gctacggtaa
     8761 ggtaaacgga aaatgccgcc cgaatcttcg gacggcattt ttcacgcggc atagcgtttc
     8821 aacatttccc gataatccgc cggttcccgt ccggcgaact caaaggcctt caaatcggcg
     8881 ttatctgatg ccttgcggct ctcgttcagc caataggcga tctgtttcct gcaaaactct
     8941 tcagcactca tcgtcaaatc caaaaatgcc tgttcaggtg cggcgcatcc tcacgcgtga
     9001 tacggcgcaa atcactgtcg gccggcaggc cgaaatacag ctcgaaacgc ctccttgccg
     9061 attccgaacg ctgcgggtcg aaagtctccg catccgggat actgaaagcc tccgccaaag
     9121 cccaataaac caaattccga tgatgtatgg aattgatttc cggctcgtcc ccgtcccccg
     9181 ccatatcccg cggcaggcaa taccccccgg caaacaacct gccgtcacgg tccggcgcag
     9241 gcgcaagcgt cagcttcccg tcgccctgca ccgcataaac cggcagcccc gtttgcgccc
     9301 cgtcgcgcca acccggggcg caaacatccg ccgcctccgg cgaaaccaac ttcaggcaca
     9361 ccggcttgcc gtcggacaca aaacggatac tgtcgatttc atacgcataa cccgccgcaa
     9421 acccccctgc cgcataagca taaagcggcc gccctttttc cacatccgtt acaaacgcat
     9481 cgtccgaatg cagcagccgg ccgcgcaggc acgcctcgtg aaccgcatcg ttcaaccatc
     9541 ccgacacctc ttcgtcgctc caaaaatacg gctcggcctt atcattggca agcacgcgga
     9601 aacgcgcaat caaagccttc aaattcattg cgcgataccg aactgacgaa ccatccgaac
     9661 cacgtccccg cgcaaattct ccgccgattt gcgcttatcc aaaccctggc cgtaattcgc
     9721 cttcgcatac agttcaagct cgtccttgcc catccggttg atttgcagga tcacatcctg
     9781 caccgcgtca aacgccgccg tttcccgttc gagttccgaa gcgcccgcct tttccaaagc
     9841 ctccgtatca tccgcctttt ccacgcgttt gcccgggacg gcggcaaaca catcgccgtg
     9901 gcgcaacaac tttgccgcag ccccggccga cacctcgcgc acctggccgt cttcgaaaac
     9961 caagcccgta tgatagatac ggtcgcgcca cacggcacgc ccgccgatat acttgataaa
    10021 aaccttatcc atccaacccc ccgccgccgt ccggacggca ttgtcaaata cagcaacgcc
    10081 gcaaaggcaa atccccgggc ggcgtttcat tccgcttaca actggccgaa cttctcgccg
    10141 taaatcaaaa tatcgatatc ggcggccttc ttattttccg cccctttaag cgtaaccgtc
    10201 agcaaagcct gcttgggcag cgtaaccagt ttggcagact ggcagcggat acgcccggca
    10261 gacgcaaagt ccgcgcccga agcaaaataa gccgcatcct gaggcacgtg cgcatcatcc
    10321 gcaccgtccg catacgcaaa gccgacatcg gcagtgatac cggaagacat cgccgtcttc
    10381 acaataatct gcgcgtcatc caaacggaaa ccctgttcca agacacccag cgccaacaca
    10441 tcgcccgcct tgggcggccc gtaggcatcc gcccccaaga ttgcaccgtc ctgcttcgca
    10501 acgatacggt aatgctccgc cgccagattg ccgtaaggca cgccgccgaa acggttgccg
    10561 ccattgtttc tcacatgaat ccgagccata aaaccccccc caactcgaca aataggaaaa
    10621 gccgcccttt caagcggcat tcgaatcagc ggataccgcc gtgcaaagga accaccgtat
    10681 ccacaaccgt tacgccgtag tcggtaattt ccttcgcacc cccgccgaca tccacggcaa
    10741 agcgcgtttt cgccacaccg cgtatcgtac cgacgagcgt ttccacacgg ttgccgtggt
    10801 caagctcctt ctcagaccaa aagaaaggca taccgccgtg tttgccggta ttcgcaaacg
    10861 cctccaacac cgcctggccg cccaaaataa ccgaacggtc gaccgcaaac ttgtccgcaa
    10921 acgaagccgg gattttcaag cccgattccg cctcgctgaa cttatcggcg caatacttca
    10981 tctcatcgcc cgcatagaaa cggatggggc gcggcatttt caccaggata aagccgttcc
    11041 acaaacccgc atcgcccaaa aacagcggat tttgatttgc ctgggaggcg cgcgcgattg
    11101 ccgaagcctg aagctggcgg aatttcggat cggcggcaaa acggttgtac tgcgccgggg
    11161 aaagcagcca cacgcgcaaa ggcgaatcac ccgccgcctt gtcgccctca aacttcacaa
    11221 tcggcggcgg cagcggaatc tggtcgagca cggtacgcat actgtccacc gtatccatcg
    11281 taaacaaatc ggtagaggca atcttcaact cgccgccctt cgccgcaacg cccgttaccg
    11341 catcgcccga agccgtaaaa tggcggtttt tggacggggc tttgaccgga ttgaccgcat
    11401 aatcattgaa tttcgggtcg cccgccaaag gcacgcccca ttcgatattg tcatgaaaac
    11461 cgcgcgcgcc cgccatatgc accaacagcg tctggtcggc ataacggtcc atcaggcttt
    11521 gcgccgccgg acgaatcagc gcgcgataat cggcagggct gcgctgattc gtcatcgtgc
    11581 cgccgccgtc aacggggaaa cgcgcctgat tcacacgcaa acccgcctcc gacaggctca
    11641 tccccacgcc cctgccttcc gccgtgttgt cgcccataat cggcagggcg gaaacagggt
    11701 tgaccaaatt gaaacggatt tcgtcaccca tgccgcgcgt caaatcctga cagcgcacga
    11761 ccggcatatg ctgggtcgtc tgtttgcgca aagtcgcctc cgcacccgcg gtaccggcag
    11821 gcatcttgcc cgccaaacgg ttcagcgtac tgttgcgctg catatgcatc gcaaacagcc
    11881 ccgccgcctg cttcatcatc gcctgcgggt cgccgtaggc cgtattcgtc ttttgtgcca
    11941 taaagagcct ccctcaaaaa ctcaagaatt taaaaacttc tcaacctgct cttccgtcat
    12001 ggaagccagt ttttccgcca gcgcgttgcc gctcaaataa tccggcgggc cgtccgaagc
    12061 cgtatggtcg cgccccgccg gaatatccga cagcgtattg ggcgtattct tcccggcagg
    12121 cggcttttcg gcgggttttt ccggttcggc ggcggatttt gccgccttaa acatatcgaa
    12181 cgccccgatg acctcggcgg cagtcccgtc cttaagcgcg tcattaaacg ccttgcggac
    12241 aagcgggttc tgcgcatcca gccactcctc cagctcgcgg ctttccacaa tcgaatccgc
    12301 atcggggtgc gccgtataga tttcgcggta atgcgccttt gccgcctcct gccgcttcat
    12361 gtccgcctca taacccgcca gcttctcctg aatcagcttt tccacgccct ttttcacatc
    12421 ctcgtcggaa aaatccccga acaacgaatt atcggaaccg tcagccgttt ccactccggg
    12481 tttttccgcc cccttctcaa gctgggcaat gcgctccctc aaggcttggg cttcggcttt
    12541 cgccgacttt tccgcctcac gcgcctctac cagcttttca taagggatag tgtgtttgcc
    12601 gtccttggca cggatgacct tgtccgcacg ccgtccgccg cgccgatacc cgcgccgacc
    12661 accgcgcccg tactgccgac caccagcgat tccgcccact tcttaccctc caaaccgtct
    12721 tggatatcgg ggaaaaccaa ctggtccaaa cccaaacgcc ccgacacgtc cagcggcgtc
    12781 aggcggctga agcccttggc caacacctcg cccgccttat ccccgaaagt gtccgccaac
    12841 atattgcgca acgccgcttc cgcatcccac gggtcatcgt cgtcactgcc caacatcgaa
    12901 gccaccgcaa gcagcgtcga cacaaacggc agccccaaga tgcccgaagc catcgcatgc
    12961 gagaccaaca gccccgccag cgtcttacgc gcctccttat cccccttaaa cgcaaggtac
    13021 gcattgcgcc ccagcgcata caggatattc tgcgaatact gcttgaacag gaagaccacc
    13081 ttcgccgcat tgcccatcat aaagcgcgga cggttttgcg ccgcatagtc aaaatgcccg
    13141 tcatacgtcg cctttttcgc ctgttcgaaa gccgcgtcgc tgtccgcccc cgcccgtttc
    13201 gccaaacggt aggccgcgac aaacgtaacc tggcggttaa acttctccgc atgatggaac
    13261 agccaggccg ccttatccat caccttctgc caagccgacc ccgccaaccc cgggtcgccg
    13321 ttggccacac ccgccaaatc atgcgcctgc gacaaatcga tcacaccgta atccgccgcc
    13381 tttcggaacg ccgccttctc gtccccgttc aaactgtcct caatcgtatt gaacttctcc
    13441 ccgaacctca gcccgatctg tttagaagcc cgcagcaatt cccgcgccgc accggcataa
    13501 ccccacttcg ccgccatcac cggataagcc accaccgccg tctgcgacag gttcacaacc
    13561 gcagaagcgg gcgacatccc catcatccac aaaaagccga agcccgtcaa agcctgcgcc
    13621 agcttggaag gattcggatt catcaccgcc tcgtggcgtt tcctcatctc atccgccaca
    13681 cgctgcaact gacgctggtc gaaaccctcc tcatatttgc gcccgtccac aaaatcctgc
    13741 atcacatcca actgttccgc catacggtcc gcatagcgca acttcgccag ataattcgca
    13801 ccgctgccca tattctgcgc atatgcgcgc ctcgcatcat cgctgaagcc cggcacgccc
    13861 ttgcggtgga tgccgtgttt cgcccaagac gtatcgggca gcgagttcaa ataaagctgc
    13921 gtcagcgtat cgttcaatcg ggcccgttgc gccggatcca aatccaacat accgacagcc
    13981 tcgcccagct ccctcataaa accgctgccg accgcatcgc ggcttcggat ataatcccgg
    14041 gacttcatca cgggcgaaac cttaaacccc ggcccgaaat cagccttcag cgcatcgcgc
    14101 agcttctccg cctcgctcaa agtttccgcg cgggacacat tcgcactatt cccgtccgca
    14161 tccttgacca ccaccagata atcgccgaaa cgcgccaagg ggaaatacac accgcccagc
    14221 tcgttgtcaa aacgctcctt caggcggcgc accgtttccg catcctgccc cgcacgcgcc
    14281 aaccgttcgg caagcgcatc gcgcacctgc ccgaaatgcg ccctataatc atcccgcgcc
    14341 ttgcggtaca ccctctgcgc cgcatcaggc agcccggcaa aacggcggtt cagcaaagca
    14401 agcccctcca aagccttttg cgccccaacc tcatcctttt ccaaacgctt cacatccgcg
    14461 cgcgcctgct ccaacatccg taccgcatcc gttttagccc ggctttccgc cgccgcatcc
    14521 gccccggcgc gcctcaacgc ccgcttcgcc tccgaatccg cataaaacag acggcgcagg
    14581 cgcatatccg cctcatccgc caaaatttcc cgaccgtgtt tttcctgcgc cttctccagc
    14641 gcataagccg ccttatccgc cgcccgttgc gccttattgt aagcagcatc cgcacgcgcg
    14701 gtacgcgcac cggcggaagc aacggccgcc cgcgccttcg cgattttacc gtccgcaata
    14761 tccaaagccg tccggatgcc gtccaaacgc ccccgggcat cctttcgcat cagcgggtcg
    14821 gcatccacct tcgccagcgt cgcatcgtgc atcagatccg caagcgcttt cgcatcctcc
    14881 tccttcaacc tgccccattc cctgacaacc ttgtccgcct ccgtaaccgc cttgttcgca
    14941 tccgcgccga agagttccga caaacgcccg tattccttca ggccgtccaa aacgccgccg
    15001 taaatgtcct cgatctgccg cctgcccaaa aactggagct gaatcccctt ggaagcatcc
    15061 cagcggtctt tcagcgcgtt tctcaaatcg gattttttac ccgttatacg gctgattgcg
    15121 gccccgatgt tcgccgcacg ggaaaaacgc accgcctccg tgtcaaaatc ggcaatgccg
    15181 cccttgcccg catctttgtc ttgcaaaatc tgcaacaggc gtttatcatc aatgcccgat
    15241 aaaggcgatt tgctttcaga ggctcccgta gttgatacgc ggtcaagtaa tgaaagcaag
    15301 ttgccttttt ctattttcgt cagcttatgg tcgtaatagc ggttaccgtt cctgctaacc
    15361 gcgacggcag cccttaccgt gtaatccgca ccgccgacat tcagtcccga aacataatat
    15421 tcataaccct gaatatcgcc gttcttagcc aaatcctcgt tcggcagcgt atcgatatac
    15481 accgcattct caataatctg cggaattgcc gcgatactct gcaaatgttc cgcgtcctta
    15541 tagtcgtgac gcaatatttc agtgatgccc gaacgtccca aactgatttc acgccccgtg
    15601 tccttattca cataaggccc gcgcaaagat ttgccgtatt ccagcgcatt gcgcttatat
    15661 tcgcgcaaat tgccgccttc cggaacctcc ctgcccgaga tacggatggt ttccgcacgg
    15721 cgcagcttct caaggctttc cgacttggaa cgttccgccg caccgtcttc ggcccgcgcg
    15781 aacatcactg cacgatgatt gccgccccaa ggcgcatccc gcgccccttc cacattcccg
    15841 tgcaaatccg ccagcatcgc aaacacatcc gcgtccgaca tcgcaccgcc ggccttgccc
    15901 gccaggcgtt gcagcacacg gcgcaacagg gcgccgatac gcgccaagac gcccgcaatg
    15961 ccgcgtttca aaccgttgcc gaccttcacg ccgtaacggt tttcaatgcc cgcccaaccg
    16021 ccggtacgct gcgccgcata aagctccgcg accgcctctt ccaccgcggc ggagcgcacc
    16081 gaggccgccg catcgcccgt gccttcgcgc ccttcctgca ccgcgtccgc aatgcgccga
    16141 atcaggccgt tgccgtccgc acgttccaat tcttcacggt acttggcgaa accatccgcc
    16201 gcaaagcccc tgtgccccag ctcgtgccac gccgcccata ccgcacgttc gggcgtaagg
    16261 ttttccgcca ccagcgtgat tttgcccgtc ctgc
//
LOCUS       DAXFHF010000053        15965 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXFHF010000053 DAXFHF010000000
VERSION     DAXFHF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15965
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.053"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..272
                     /locus_tag="ACFIRM_001565"
     CDS             <1..272
                     /locus_tag="ACFIRM_001565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029161.1"
                     /translation="TDPKTNHILKQIISKSDFFKIKFQKKETGKIRHKTLTYSKPSVL
                     QRKYPKKTICLPSVSNRYTAGTRYFSDGAAVVRTDKNVTAAERIA"
     gene            326..2710
                     /gene="ppsA"
                     /locus_tag="ACFIRM_001566"
     CDS             326..2710
                     /gene="ppsA"
                     /locus_tag="ACFIRM_001566"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC3029162.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2931..4190
                     /gene="rho"
                     /locus_tag="ACFIRM_001567"
     CDS             2931..4190
                     /gene="rho"
                     /locus_tag="ACFIRM_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC3029163.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4805..6109
                     /locus_tag="ACFIRM_001568"
     CDS             4805..6109
                     /locus_tag="ACFIRM_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC3029164.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6203..7498)
                     /locus_tag="ACFIRM_001569"
     CDS             complement(6203..7498)
                     /locus_tag="ACFIRM_001569"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC3029165.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLCQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7625..8512)
                     /locus_tag="ACFIRM_001570"
     CDS             complement(7625..8512)
                     /locus_tag="ACFIRM_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3029166.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8512..9477)
                     /locus_tag="ACFIRM_001571"
     CDS             complement(8512..9477)
                     /locus_tag="ACFIRM_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3029167.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9493..10617)
                     /gene="potA"
                     /locus_tag="ACFIRM_001572"
     CDS             complement(9493..10617)
                     /gene="potA"
                     /locus_tag="ACFIRM_001572"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC3029168.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10895..11164)
                     /gene="rpsO"
                     /locus_tag="ACFIRM_001573"
     CDS             complement(10895..11164)
                     /gene="rpsO"
                     /locus_tag="ACFIRM_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC3029169.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11245..11379)
                     /locus_tag="ACFIRM_001574"
     CDS             complement(11245..11379)
                     /locus_tag="ACFIRM_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029170.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(11369..12304)
                     /gene="secF"
                     /locus_tag="ACFIRM_001575"
     CDS             complement(11369..12304)
                     /gene="secF"
                     /locus_tag="ACFIRM_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC3029171.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12308..14161)
                     /gene="secD"
                     /locus_tag="ACFIRM_001576"
     CDS             complement(12308..14161)
                     /gene="secD"
                     /locus_tag="ACFIRM_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC3029172.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14372..14638)
                     /gene="yajC"
                     /locus_tag="ACFIRM_001577"
     CDS             complement(14372..14638)
                     /gene="yajC"
                     /locus_tag="ACFIRM_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC3029173.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14769..15887
                     /locus_tag="ACFIRM_001578"
     CDS             14769..15887
                     /locus_tag="ACFIRM_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC3029174.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPSCTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPEHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGNRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4023 a         4356 c         3990 g         3596 t
ORIGIN      
        1 gtacagaccc aaaaacaaat catattttaa aacaaattat ctcaaaatca gatttcttta
       61 aaataaaatt ccaaaaaaaa gaaacaggaa aaatacggca taaaacatta acctattcaa
      121 aaccatctgt tttacaaagg aaatacccga aaaaaacaat atgcctgcca tcggtttcaa
      181 accgctacac agccggaacc cgctattttt cagacggcgc agcggttgtc cggacagata
      241 aaaacgtgac agcggcggaa cgaatcgctt aaaataagca agcggatttg tttctttttt
      301 aacatatttt ggattggaca cacaaatggc cgacaactac gtaatctggt ttgaaaacct
      361 gcgtatgaca gatgttgaac gcgtgggcgg taaaaacgcc tcgctgggcg aaatgatcag
      421 tcaactgacc gaaaaaggcg ttcgcgtccc cggtggcttt gccaccacag ccgatgccta
      481 ccgcgcattt ctcgcacaca acggtctgaa cgaacgcatt tccgccgctc tggcaaaatt
      541 ggatgtcgaa gacgtttccg aactggcacg cgtcggcaaa gaaatccgcc aatggatttt
      601 ggacacgccc ttccccgaac agctcgatgc cgaaatcgaa gcggcatgga acaaaatggt
      661 taccgatgcc gacggcgcgg acatttccgt tgccgtacgc tcttccgcaa ccgccgaaga
      721 cctgcccgac gcatcatttg ccggccaaca ggaaaccttc ctgaatatca atggcttgga
      781 taacgttaaa aaagcgatgc gccacgtttt cgcctccctg tacaacgacc gcgccatttc
      841 ctaccgcgtc cacaaaggct tcgaacacga catcgtcgcc ctttccgccg gcgttcaacg
      901 catggtgcgt tccgacagcg gcgcatcagg tgtgatgttc accctcgaca ccgaatccgg
      961 ctacaaccaa gtcgtctttg ttacctcctc ttacggtctg ggcgaaaacg tcgtacaagg
     1021 cgcggtcaac ccggacgaat tttatgtgtt caaacccacg ctcaaagcag gcaaacccgc
     1081 catcctgcgc aagaccatgg gttcgaaaca catcaaaatg attttcaccg acaaagccga
     1141 agccggcaaa tcggtaacca acgtcgaagt ccccgaagaa gaccgcaacc gcttctccat
     1201 taccgacgaa gaaatcaccg agttggcaca ctacgcgctg accatcgaaa aacactacgg
     1261 ccgcccgatg gatatcgaat ggggacgcga cggcctggac ggcaaactct acatcctgca
     1321 agcccgcccc gaaaccgtca aatcccaaga agaaggcagc cgcaacctgc gccgctacgc
     1381 catcaacggc gaaaaaaccg tcttgtgcga aggtcgtgcc atcggtcaaa aagtcggtca
     1441 gggcaaggtg cgcctgatta aagatgcttc cgagatggat tccgtcgaag ccggcgacgt
     1501 actggttacc gacatgaccg atccggattg ggaacccgtg atgaaacgtg cttccgccat
     1561 cgtgaccaac cgcggcggac gcacctgcca cgccgccatc atcgcgcgcg aactgggtat
     1621 tcctgccgtc gtcggctgcg gcaatgcgac cgaactcttg gaaaacggtc aggaagttac
     1681 cgtatcctgt gccgaaggcg ataccggctt tatctatgcc ggcctgctgg acgtacaaat
     1741 taccgatgtc gccttggaca atatgccaaa agcacccgtc aaagtcatga tgaacgtcgg
     1801 caatcccgaa ctcgcattca gcttcgccaa tctgcccagc gaaggcatcg gcttggcgcg
     1861 tatggaattt atcatcaacc gccaaatcgg catccacccg aaagccttgt tggaatttga
     1921 caaacaagac gacgaactga aagcggaaat tatccgccgc atcgccggtt acgcatcccc
     1981 tgtcgacttc tacgtcgata aaatcgccga aggcgtggca acgcttgccg catcggttta
     2041 tccgcgtaaa accatcgtcc gtatgtccga cttcaaatcc aacgaatacg ccaacctggt
     2101 cggcggcagc gtatacgaac cgcatgaaga aaacccgatg ttgggcttcc gtggtgcggc
     2161 gcgttatgtt gccgagagct tcaaagactg tttcgccttg gaatgcaaag ccttgaaacg
     2221 cgttcgtgac gaaatgggtc tgaccaacgt cgaaatcatg attccgttcg tccgcacttt
     2281 gggcgaagcc gaagcagtgg ttaaggcatt gaaagaaaac ggcttggaac gcggcaaaaa
     2341 cggcctgcgc ctgattatga tgtgcgagct gccgagcaac gcggtattgg cggaacaatt
     2401 cctgcaatac ttcgacggct tctccatcgg ctcgaacgac atgacccagc tgaccctcgg
     2461 cctcgaccga gacagcggct tggtgtccga atcgtttgac gaacgcaacc ctgccgtcaa
     2521 agtgatgctg catcttgcca tctccgcctg ccgcaagcag aacaaatatg tcggtatctg
     2581 cggtcaaggt ccgtccgacc atccggactt tgccaaatgg ctggttggag aaggcatcga
     2641 aagcgtgtct ctgaacccgg ataccgtcat cgaaacttgg ctgtatttgg cgaatgaatt
     2701 gaacaaataa tcaattccca tacccccaag cctgaaaagc gcgggggtat tttttccaag
     2761 cagctccgtt cagacggcat ttcctgtcga tgccccgtcc gcgataatat ttgacaccca
     2821 cgcgccgact gcctacaatt cccccctctc cgagcaaccg gcaacagtca gcttcttctt
     2881 tcagacggca tccacccgtc ttttcctttc tattctatat atcattgatt atgcacgtct
     2941 ccgaattaca aaccctgcac atttccaaac tcttagaatt ggcggaagaa cacggcatcg
     3001 agaacgccaa ccgattccgc aaacaagacc tcgtatttgc catcgtccgc cagatgatga
     3061 aaaaaggcga gggttttacc tgctccggca cgctcgaaat cctgcccgac ggcttcggct
     3121 tcctccgcag cgcggacacg tcctatcttg ccggccccga cgacatctat gtctcgccca
     3181 ctcaaatccg ccgcttcaac ctgcatacgg gcgacaccat cgaaggcagc gtgcgcgtcc
     3241 ccaaagacaa cgaacgctat tttgccttgg tcagactgga ttccatcaat ggcgaccacc
     3301 cggaagtatg ccgccataaa atcctgtttg aaaacctgac cccgctgttt ccgaccgaac
     3361 agttgaagct ggaacgcgac ttaaagtccg aagaaaacct gaccggacgc gccatcgacc
     3421 tgatttcgcc catcggcaaa ggtcagcgcg ccctcttggt tgccccgccc aaaatcggta
     3481 aaaccgtgat gctgcaaaac attgcccacg ccgttaccgc aaactatcct gaagtcgaac
     3541 tcatcgtcct cttgattgac gaacgccccg aagaagtaac cgaaatgagc cgttccgtcc
     3601 gaggtgaagt agtctcctcc acctttgacg aaccggcgca acgccacgtc caagttgccg
     3661 aaatggtgct tgaaaaagcc aagcgtatgg tggaacacaa aaaagacgtg gtcatcctgc
     3721 tggattcgat tacccgcctt gcccgcgcct acaataccgt cgtccccgcc tcgggcaaaa
     3781 tcctgaccgg cggtgtcgat gccaacgcgc tgcaccgccc caaacgtttc ttcggtgcgg
     3841 cgcgcaacgt ggaagaaggc ggttcgctga ccattatcgc caccgcattg gttgaaaccg
     3901 gcagccgtat ggacgatgtg atttacgaag aattcaaagg taccggcaat atggaattgc
     3961 acctcgaccg ccgtatggcg gaaaaacgcc tcttccccgc catcaacatc aacaaatccg
     4021 gcacgcgccg cgaagagctg cttgtgccga acgaccagtt gcaacgtatg tggttactgc
     4081 gcaagttcct gcacccgatg gacgaaatcg aagctaccga gttcttaaac ggaaaaatca
     4141 aagcatctaa aaataatgat gatttctttg aactgatgcg cggaaaataa acgcgccgcc
     4201 cgcattaatg cgaaatgccg tctgaagcct gaaaatcggg tttcagacga cattttcatt
     4261 cgcacggccg gcgcagctcc cccccccgct aacggcgcaa ccgccggcag tgtccgtgtc
     4321 cgcctgccga aggcgcggcc tttgcaaaac cggctcgaac gcatccctac cggcaatgaa
     4381 aaccgatgcg ggaacttgat tcaaggttgc gccgaaccgc gcccattttt gtataaattt
     4441 tattttttat ttccaattag aaatttttaa tatattaatc aataaattaa ttttataaaa
     4501 taaaaatatt gtcaacaatc ttttgactta tcgcccaaac ctctgtatat tttcctacag
     4561 taaattgttg acaatccata cgcccgcata tgcgccgcct aaggataaat cctcccgccg
     4621 gacaacgggg gcaagggata ggatgcgata tttccatatt caaacaaggg atttgtttca
     4681 cgcacagggc ggcacatcgg caaaatcccc gcgccggtcc ggcagggctt gcgcccatcc
     4741 cggacaagcc ccgaccccgc ctttccgaaa gacgggccca accattaagg aaactttaat
     4801 caaaatgaaa aaacacatat gggcggcatc tttgctgccg gcatccctat cggcagaacc
     4861 tttaaactgg tggaaacctt attccgccgt caattcgggc gataccgcct gggtgatgac
     4921 tgcggctgcc ttggtactgt tgatgacgct tcccgggctg gctttattct acggcggtat
     4981 ggtgcggaaa aaaaacctgc tctcgacgat gatgcacagc ttttccatcg cgacattggt
     5041 gggcatcctt tgggtcgccg tcggctattc tttggcgttc acgccgggaa atgcctttat
     5101 cggcggtttg gggcgcgtat ttttaagcgg gatgcagata gacgctgccg cacggatgct
     5161 gaccgtgtcg cccaatgcgc cgactgttcc cgaaccggta tttatgtttt ttcagatgac
     5221 gtttgccatt atttcgactg ccattattac cggcgcgttt gccgaacgaa tgaaatattc
     5281 ggcaatgatg ctgttttcgg gcatatggtt tttattggtt tatgtgccgg gcgcgcattg
     5341 ggtgtggggc ggcggcttta tgagcaaggg cggcgtattg gattatgccg gcggtacggt
     5401 ggtgcacatc aatgccggta tcgcgggact cgtcgccgcc ttggttttgg gcaggcgcat
     5461 aggctacggg cgcgaggcga tgcctccgca caatatggcg atgacgctga ttggcgcggc
     5521 aatgttgtgg ttcggctggt tcggctttaa cgctggttcg gcgcttgcgg cagacgcggc
     5581 ggcggggatg gcgatggcgg taacgcaggt gtcggccgta ttcggcgcgg caggctggct
     5641 tgcctgcgag aaaatagcgg gacacaaacc ttctgctttg gggctggctt ccggcgcagt
     5701 ttccgggctg gtcggcatca cccctgccgc cggctttacc ggtccgtcgg gcgcggccgc
     5761 catcggtata ttgactgccg ccgcgtgctt tgtgtccgtc accgtcgtca aacacaaatt
     5821 gcgttacgat gattctttgg acgctttcgg catacacgga ttcggcgggc tggtaggcgg
     5881 aatattgacc ggcatctttt tcgacaaccg cattttcggc ggggatgcgg cagtttggca
     5941 gcagttgtgg atacaggtaa aagacgggtt cattatggcg gcatacagcg ggctgatgag
     6001 ttgggcgatt ttgaaggctg tggggaaaat ctgcggcggt ctgcgcgtcg gcaaggatgt
     6061 cgaacgcgaa ggtttggatc tgaatatcca cggcgaacac gtggaataag gacggctatg
     6121 ccgtctgaag cctgaaaatc gggtttcaga cggcattttt cacgtttgcc gccgatgggg
     6181 ataaacatac tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga
     6241 ggaaagaacc gagtttgcgc aaaaacggtt gcaggataat attcggctgg cacaactgtt
     6301 caaacggttt cagacggcat tcgtccccta aaattgcttc ggcaaccgcc aggcctgcaa
     6361 tgcccgttat cgccatcccg tgcccggaat aaccttgcgt ataaaaaaca ttcgaggtca
     6421 aacgtccgaa atgcgggaca aggttggcgg taatatcgca ctccccgccc cacgaatatt
     6481 cgattctaac atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg
     6541 tcatacgcgc aggattgtcg ataaattcgt tatccttacc gccgaaaagc agcctgccgt
     6601 ccgcgctgag gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttgc
     6661 ggataagccc ttttgtgcgc gcgcccaagg gttcggtggc aataataaag gtgctgacgg
     6721 caatcgcctt gcgttccaaa ggccggaata tcgggttcaa accgacataa gtattgacgg
     6781 catagaccac atttttacac tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt
     6841 gatacggttc gatgcgcgtc atcggggatt gctcgaaaat ctgcgcgccg gcttcggcag
     6901 cggcgctggc aacacccaac gtgtaattga gcggatgaag atgcccggac aagggatcga
     6961 actgtgcgcc ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa
     7021 gttgataatg actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt
     7081 cccaatgctg cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcgatgg
     7141 catgtttgcg gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc
     7201 attgcgcctg ctccaagccg acctgttttt caatttcccc cataccgcag gcgtagtcgc
     7261 tgataacctg cccgccactc ctgccggacg cgccgaagcc gatacgtgcg gcttccaaaa
     7321 cgacggcttc atgtccgtgt tccgccagcg gcaatgcggt acacaaaccg ctcaaaccgc
     7381 cgccgataat gcaggtttcg gctttcagac ggcattggag tttcggataa acagtatgcg
     7441 gattaaccga actaaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg
     7501 tgtttgcttt atcgggtata ttttcggacg gaatgataca gactgtcggg ccatatcgtc
     7561 caaacagaaa atcggttgaa aaaaaacagg ctgacccagt catgcggtca gcctgcctta
     7621 ttaattaatt tgctttctcg gcagccaatt tttcttggcg gtaggcttct gccgcttctc
     7681 ggttgcgctt ggttgcctgc ctcatcatcc aataattgac gatgatgacc aatgttccga
     7741 tgatgccgat caggatggtt gccaagacat tcatctgagg atcgagaccc aacttgatct
     7801 tggagaaaat aacctgcggc aatgtggatg aaccggggcc ggagaggaat gaggtaatta
     7861 ccaaatcatc caaagacaag gtaatgccga gtaggaagcc tgaagcaatg gcaggggcaa
     7921 tcaaaggcaa agtgatgaca aaaaagattt tcagcggacg cgcgcccaag tccattgcgg
     7981 cttcttcgag cgactggtca agctcgacca gccgcgaacg gatgacaacg gtaatgtatg
     8041 ccatgcacag cgtcgtatgt ccgaggaaga tggtaaaaaa gccgcgatcg aagtagagat
     8101 gttgcaacca ttcgctgccc tgcaaaaata tctgcacctg aataatcagc agcagcatag
     8161 acagaccggt aatcacgtcg ggcatgacca tgggcgcgga aatcataccg gcaaacagcg
     8221 tgctgccgcg aaagcgtttg atacgcgcca tcgcatagcc cgccagcgtg cccaaaacga
     8281 cggcggcaag cgaagacaca acggcaatcc gcagcgacag ccaagcggct tccaaaatgg
     8341 tgtcgttttc cagcaatgcg ccgtaccact tggtcgaaaa gccgccccaa acggtaacaa
     8401 gtttggattc gttgaacgaa tagatgacca aaacaaccag cgggatatac agaaacgcca
     8461 gcgatagtgc caacatcagt ttcaagaacc aagataattt ggatttctgc attatttggc
     8521 tccttcttcc aattcgcggt tttcatagtg ctggaacagg gcaatcggta caaccagtag
     8581 cgcgaccatc acgacggcga cggcagaagc caacggccag ttgttttgat caaagaatgc
     8641 ctgccacaat actttaccga tcatcaggtt ttccgaaccg ccgaccagct cgggaatgac
     8701 gaactcaccg acagcgggga cgaaaaccag catcgaacct gcaataatgc cggttttcga
     8761 caaaggcagg gtaatcgtca agaacgattt gaccggtccc gcgcccaagt cggaagccgc
     8821 ttcgagcagg cggctgtcca gtttcaccag ctgcgtgtaa agcggcagaa tcataaacgg
     8881 cagataggca taaaccatta ccaaatttaa cgaaaacgca ttatagaaca aatctaatgg
     8941 ttctctgata atacccatct taatcaataa gttgtttatg atgccgttat gcccgagcaa
     9001 acccatccac gcatagacac gcagcaggaa cgatgtccaa aaaggtaaca tgatggcaag
     9061 cagcaaacca ttgcggacgg aaggattggc acgggaaatc gcataggcgg tcgggtaacc
     9121 gaccaacagg caaatcaccg tcgtcgtcag cgcagtctta attgaagacc aataagtcat
     9181 cagatagata ttactgtttt cactgtcgcc gaaggggtta agggtatgcc aaaaattttg
     9241 gaaaatatcg gcatagtttt gatagctgac ggcgatgttc aggcgaccca aatcctcatc
     9301 tatcgtcgtt aaaggagtaa acggtggaat ggcgatttct tgttcggcaa agctgatttt
     9361 cagcacgatg gcgaacggaa tcagaaacag caccaaaagc caaatatacg gtacggcaat
     9421 caccgcacgc tgccccggac ggcggaacag tttgtttttc agtttattaa ggttcatctc
     9481 actcccctta aatcaacgga acaacggagt cggttggttt tccggccagc tgatatagac
     9541 ggtttcatcc caagtcggcg gcgtgatgtt acgcacatac cagtaaggtg caggaacttg
     9601 gcttttgacg acgcgcccgt tggcgagttt gatatggtat atggcgaagc tgcccaaata
     9661 ggcaatttct tttaccgtgc ctttcgccca gttgtagtcg cccaaatatt cgggtttttc
     9721 tttatataaa tcaatatcct ctggtcgaat actaacccaa agatcctgct cgctcgggcc
     9781 acccaaacca tggtcgatac ggacgtggtt ttccaaacct tcgcattcga taacggcata
     9841 gtcggcatga ttttcaatca ccacaccgtc aaagatgttg gtttcgccga taaactcggc
     9901 agtgaagcgg ctgttgggat agtcgtacac gtcgctgggc gtgccgacct gctgcaactg
     9961 accgtcggac ataatggcga tgcgggtcgc catcgtcatc gcctcttctt ggtcgtgcgt
    10021 aaccataatg caggttacgc cgacttgttc cagcgtattg accaactcga gctgggtttg
    10081 ctggcgcaat ttcttatcca acgcccccaa aggttcgtca agcaacagga ttttcggacg
    10141 ttttgccaaa ctgcgcgcca aggcgatacg ttgctgctga ccaccggaca attggtgcgg
    10201 tttgcgtttg gcaaatttgg tcatctgaac cagacgcagc atttcttcga cgcgcgcatc
    10261 gatttcgcct ttaggcattt tgtcctgttt cagaccgaag gcaatgtttt gctccaccgt
    10321 catatgcgga aaaagcgcgt aactttggaa catcatattg atggggcggt catagggtgc
    10381 aagtttggta atatcttgac catcaaggat gatttttccc tgattgggac tttccatacc
    10441 cgccagcata cgcagcagcg tagattttcc gctgccggaa ctgcccaaaa gggcgaagat
    10501 ttcgttttga taaatgttca agtcgatgtt atcgacagcg taattgtcac caaacttttt
    10561 caccaaacct tggatttgca gataaggttt ggctgaagac gctgtggttg cggtcataat
    10621 ggcaatactc caataaaaag acgagtaccg gcaaaacgga ttttcgaatg ggtgataaaa
    10681 agctgtttga ttgctggcgg gagtcgcaac gtctgatgcc gtctgaaact cttgtgaagc
    10741 gcacgggcag catgaaataa aaaggggttc taaagaaccc cgcattatat tagtttataa
    10801 ggtttttagg gtaataaata tgggtttgag cttgtcggaa agcaacagaa aagaaaacac
    10861 cgcccatttt tctgggcggt gtcggaaagc gcaattattt acgcagaccc aagcgggtaa
    10921 tcaacgcgcg atacgtatcg ggctgggtac ggcgcaaata ggccaacagg cggcggcgtt
    10981 ggctgaccat tttcaacagg ccgcgacggc tgtggtggtc tttggggttg gctttgaagt
    11041 ggggggtcag atcgttgatg cggaaagtca acagggcaac ttgtacttca gaagagccgg
    11101 tgtcgccttc tttacgttgg aaatctttaa cgatttgtgc tttttgttct acggtcagtg
    11161 ccataatgga aaaactccga aaaataaaaa gaaccccaaa tgggattcgg acaagtttgc
    11221 caagcctcga caacggcaaa ctccctattc ctgaaacagg aacagcgcat tatcacacaa
    11281 tttttgctgt tctgcacgat attttgcgat gtcggcgaaa tgccgtctga aaacggagac
    11341 atcttccggt ttcagacggc atttgacatc agaccacaat ttcttccttc tgtttcgctt
    11401 ccttggcgat attgtcgcgg ctcaaaccga acatcagcaa gagcgggctg gcaaccaata
    11461 cggaagagta gatgccgaac acgataccga tggtcagtgc catagaaaag ccgtgcaagg
    11521 ctgcaccgcc gaacaccagc atagaaacaa ccatcgcctc ggtcgaaccg tgggtaatga
    11581 tggtgcggct catcgttgcg gtaatcgcgt tgtcaatgac ttccggcacg gtatgtccgc
    11641 gcatcgccgg cttgcggaaa ttttcacgga tacggtcgaa gacgacgacg gattcgttca
    11701 cagaatagcc caatacggca aggatacctg ccaagacggt cagcgaaaat tcccattgga
    11761 agaaggcaaa gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata
    11821 cggcaaaacg ccattcgaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac
    11881 ctaaagccat caatccatta tttaccaatt cctcaccgac ttgcgggccg ataaattcga
    11941 cttggcgcaa agtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca
    12001 actgtgcgga agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac
    12061 ccaatgcctg aacctgtaca tcacccattt tcagcgtatc gaggcgttcg cgcatcttat
    12121 tgacatccgc accctgctga tattggactt ccattaccgt accgccggta aattcgacag
    12181 agaaattcag gcctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa
    12241 tgaaggttgt cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt
    12301 ccatagctta ctccttacct gccgcagttt cggcttcagg tttccatacc gcaccgatgg
    12361 aaatgttctg caatttgcgg cggcggccgt accacagatt gaccaacgca cggaatacga
    12421 cgacggacga atacatcgaa gtcagaatac ccaagcagtg tacgacggca aaacctctta
    12481 ccggtccgga accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttcgaat
    12541 caacaatggt cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc
    12601 cggcacgcaa ttcctctcgg atacgttcgt taatcaagac gttggagtcg attgccatac
    12661 ccaaagtcaa cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca
    12721 aaataccgat taggaacagt atgttggcac tcaatgcaat agtagaaaag aaacccatca
    12781 ggcggtaata aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga
    12841 agcctttttc gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct
    12901 gcatcggtgc ggcaagagaa ccggcacgca acagcaaaga cgtatcgttg gcttcggctg
    12961 tcgtcatgct tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggtg
    13021 cggttacaac ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat
    13081 ttgcggcagt cagttcgccg aaaatgctgc cgcccgcgct gtccaagctc agactgacgg
    13141 caggtgcgcc catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg ccggtcagct
    13201 cgacctgttt gctgatcagc agagtttcgg gatgctcccc gccgcttgaa agcagctcat
    13261 aaccgctcgg cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca
    13321 tgcggacttc caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcttgca
    13381 cgccgggaag ctgtacgaca atgcggtcca aacctgactg ttggataatc ggctctgcca
    13441 cacccaattc gtttacacgg ttatgcagtg tagtgatgtt ttgcttcacc gcatctgatc
    13501 gtactttatt caccgcctct tccgaaagcg ccaatacaat attgctgcct tctgaattta
    13561 acgttgcctc aggaaacagc ttgagtaact gaggcagtgc cttttgcaca tcgccggcat
    13621 cctgcaaagg gacggtcagg ctgttttcag cctgacgcac cgtgccgctg cggatttttt
    13681 cacggcgcag ttcgcggcgg atgtcgcccg aataacgctc aaacgttttc tgcatcgcgg
    13741 ctttcatatc gacctgcatg gtgaaatgca cgccgccgcg caggtccaaa cccaaaaaca
    13801 tcggattggc tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg
    13861 taatataccc ttcgcccaaa gtgttttcga taacgtcgcg cgctttaagc tgcgtttctg
    13921 tgtctttgaa acgcactttc agtgaattat ccacaacaaa catcccgtcg gtctgaatgc
    13981 ccgcgttttt cagcgcagca tccactttgg attgagtctg ttcgttgatg atgatggctt
    14041 gtcggttggt cgatacctgc acggcgggcg tttcgccgaa taggttgggc agcgaataca
    14101 ctgcggcaac cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca
    14161 tcattgttcc ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc
    14221 agaagaggca aaacccctcc ctgccaggca agaccgaaag gcagcatcct gaatatgccg
    14281 ccctgcgtgt cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc
    14341 gggctttccc gatgcggctt gtcagcacaa atcaatcgac ttttgcggca atcgcattgc
    14401 gttccacttc gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc
    14461 cgactctggt taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccggctttca
    14521 aggcggcaag cattgcctga tgcgctttga atttcttttg ttgggggcgc atgatcagga
    14581 agtagaacac caccataatc aacaccaaag gagcaaattg tgcaacagct tgattcataa
    14641 tttatccgtt ctttctaaat atgattgaaa atcgagcggg gtatataata gcataagacc
    14701 gtaaacaata tatcgggttt gccgcccata cccaaccgta tgccgtctgc accccctttc
    14761 ccgaacccat gccgctgacc cgcctgttcc tgaaactcta cgccctgttg cgcctttttt
    14821 tgggcaaaaa cgcccagacc gcctggattt tccacccctc ctgcaccggg cacgaccccg
    14881 gggcaaacca tcccgattcg cccgaccgca tcctctgcat cgagcaggca ttgcgccgcg
    14941 ccggtatttg gcagcacctc caaaccgtag aggcggaaga aatcagcgat acgcgccttg
    15001 cacttgtccg ctcgagcaaa tatctgaacc gtttggaatc ttgcctaccc gaagacggca
    15061 agatttgccg tttggacgac gacaccgtta tcagcaaaaa ctccctgtcc gccgcccgtt
    15121 tttccgccgg ttcggcagtt caggcagtcg atatggtcat gaaacgcaaa gcgtggcacg
    15181 ccttttgcgc cgcccgcccg ccggagcatc acgcgaaaag cggcaaagcc ggcggattct
    15241 gcctgctgaa caacgttgcc gccggcgtca tgcatgccat tgccgaatac cgcctgaaac
    15301 gcattgccgt catcgatttc gatgtccact acggcgacgg tacggcagaa atattcaaag
    15361 acgatccgcg catcctgttt ttcaacctgt ttgaaaccga ccttttcccc ttccccgaaa
    15421 acaacggtat gcccgacggc ggcaatatgg tgcacctgcc cttgccgtcg ggaacgggca
    15481 accgcacatt ccgcgaagcc gtccgcagac agtggctgcc ccggcttgcc gcattcaaac
    15541 ccgaactggt gctgctgtcg gcaggattcg acgcacaccg tctggacgaa tcgggcaggc
    15601 tcaacctgca cgaggcggat tttgcctggc tgacacacaa aatcattcag accgcatcca
    15661 gctgccccgg caaaatcgta tccgtactcg aaggcggcta cacccttgaa cctctggcac
    15721 aatctgctgc cgagcatatc cgcgtactgg cggggctggg caaatccgat gccgcaaccg
    15781 cctatcagaa aacactgaac ccaacaaaaa aacggtttgc caaaccgaaa accggacagg
    15841 tgcgccaacc tacccaatcc gaccgttacg atataaatgc cgtctgaagc cgattcggtt
    15901 ttcagacggc atcattattt gacctgacgc aaacagaaag ccttccgcgt gcttaaacac
    15961 atctc
//
LOCUS       DAXFHF010000054        14413 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXFHF010000054 DAXFHF010000000
VERSION     DAXFHF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.054"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..376
                     /locus_tag="ACFIRM_001579"
     CDS             <1..376
                     /locus_tag="ACFIRM_001579"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3029175.1"
                     /translation="ERFIARDIIRFPGFVNNVSEVIKAHHIFVLPSYREGVPRSTQEA
                     MAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENRAAVRLMGNASY
                     AIAKDKFDAEKVDLKFLDILKA"
     gene            complement(778..1628)
                     /locus_tag="ACFIRM_001580"
                     /pseudo
     CDS             complement(778..1628)
                     /locus_tag="ACFIRM_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1977..3491
                     /gene="katA"
                     /locus_tag="ACFIRM_001581"
     CDS             1977..3491
                     /gene="katA"
                     /locus_tag="ACFIRM_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC3029176.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            4007..4573
                     /locus_tag="ACFIRM_001582"
     CDS             4007..4573
                     /locus_tag="ACFIRM_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC3029177.1"
                     /translation="MSLYALLLVALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEAFMPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKQES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAVIGMAATVMVTIGLTAGKAFGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
     gene            4795..5958
                     /locus_tag="ACFIRM_001583"
     CDS             4795..5958
                     /locus_tag="ACFIRM_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC3029178.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(6539..8575)
                     /locus_tag="ACFIRM_001584"
     CDS             complement(6539..8575)
                     /locus_tag="ACFIRM_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC3029179.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDGRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSESDECR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSTDAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8663..9535)
                     /locus_tag="ACFIRM_001585"
     CDS             complement(8663..9535)
                     /locus_tag="ACFIRM_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC3029180.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIRFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            9801..12191
                     /gene="gyrB"
                     /locus_tag="ACFIRM_001586"
     CDS             9801..12191
                     /gene="gyrB"
                     /locus_tag="ACFIRM_001586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC3029181.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            12290..13675
                     /locus_tag="ACFIRM_001587"
     CDS             12290..13675
                     /locus_tag="ACFIRM_001587"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC3029182.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(13746..13844)
                     /locus_tag="ACFIRM_001588"
     CDS             complement(13746..13844)
                     /locus_tag="ACFIRM_001588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC3029183.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            14141..14290
                     /locus_tag="ACFIRM_001589"
     CDS             14141..14290
                     /locus_tag="ACFIRM_001589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029184.1"
                     /translation="MNQIIFAETLKNLPYLPDRHQDFVVVTEKNILKVRIPNQSLKYR
                     NHIRF"
BASE COUNT         3401 a         3886 c         3916 g         3210 t
ORIGIN      
        1 agaacgcttt atcgcccgcg atattatccg tttccccggt tttgtgaaca atgtttccga
       61 agtgataaag gcgcatcata tattcgtatt gccgtcttat agggaaggcg ttccccggag
      121 cactcaggag gcaatggccg tcggcagggc ggtgattacg acggatgtcc ccggatgcag
      181 ggaaacggtt gccgacaagg tcaacggctt cctgatcgaa ccttggaatc cccgcatctt
      241 ggccgaaaaa atgatttatt ttatcgaaaa cagggctgcc gtccgcctga tggggaatgc
      301 aagttatgcg attgccaaag ataaattcga tgccgaaaaa gtcgatttga aatttctcga
      361 tattttgaag gcgtaaacaa ggtggtcggc ttttgagttt cggtgatttc tgacaaactc
      421 ccgccattcc cgcgaaaccc cgtcattccc gcgaaagcgg gaatccaggt tcgttgagtt
      481 tcagatgtgt agggcgggct gtagggtggg cttcagccca ccgaaaccaa tcccgccacc
      541 aacctcaccg gctcaaaccg gcacagcaac ttatatcccg tcattctcgc gcaggcggga
      601 atccagcccc gccggtacag aaacttatcg aaaaaaagtt tcttcagatt ttacgttcta
      661 gattcccact ttcgcgggaa tgacgggatg caggtttccg cgcggacgga ttcggcattc
      721 ccgctttcgc agaaatgtcg gcttttggtt tgttgtcttt ataagattat tcggtaatta
      781 ttctgcggtt ttgcgtttgt gtgctgccgg aatctcaagg gattctctgt atttggcaac
      841 ggtgcggcgg gaaacctcta taccgcggaa agccagcagg ttggcgagtg cctcgtcaga
      901 ataggacttg ggcgtggacg aggcaaaacc ggcggcggga aaggggttga gcgaagcaat
      961 gaggccgagt gcggcttcga gtgtgccgct gtcggtttgg ggcaggcgtt ttttattcgc
     1021 gccggggttt ggctgcggtt gccgtcaatg ctgtcgaggg cgtttcggac gatatgcagg
     1081 gcggcgggtt tggcagcaca ttcgcccgat ctttctatct gcaatatcaa cgattcggtc
     1141 acatcggcgg cggccatgcc tgccggatcg aattttttca atgcggtcag ggcttgtttc
     1201 agcattgctt catccaacat ccactccaag ggcgtatggt ccaggatgtc ttcgatgctg
     1261 tcggtcaggt agccctgctc gtcaaggaaa tcgataagga tgtggacgca ggcggcttct
     1321 tggtcggaaa gggggtgttc gcacgcttgc gcgtgcaggt attgcttgaa atcttcttcg
     1381 ccggcgatgt tggacagcat atcttcgcct tcgtctccgc cgatttgacg cgcgggcgcg
     1441 gtgtaatggc tgaattcggc atcggaaaat tcatccgttt ctttgcgttc gagcaggggg
     1501 ttgtccgacg gccagttttc gacctcgcgt tcaagttcga tacccggcat ctgcaatacg
     1561 cgcaaagatt gttgcagccg ctggtcgagc tgctgggtct gtttgagctt tattccgatt
     1621 aaggtaatga taatgcggtg aaaattgtta ttttcagcct gtcggcgcaa aaaaatgccg
     1681 caaagcgtca ttgcattata aatggtttta atgagcgggt tcggattccg tccgataaca
     1741 aaaaacaaac gaaaatggaa acttgattgc ttataacaat attaaatcaa tttcatagtt
     1801 ttaataacga aaatcttggc gtataatcgc atccatagtt tttgcaaaag gaaaataaaa
     1861 tgtcgattca agaaatttat tacaatcaag aaaccggtta cgaatacgct ttccgccaaa
     1921 tcgtactgta agccgtttgc ccccatttga ccaacctgag aaaaggaaca agagcgatga
     1981 ctacctccaa atgccccgta acccatctga ccatgaacaa cggcgcgccc gttgccgaca
     2041 atcaaaacag cctgaccgcc ggcccgcgcg gccccctgct gacgcaagat ttgtggctga
     2101 atgaaaaact cgccgacttc gtgcgcgaag tcatccccga acgccgtatg cacgccaaag
     2161 gttcgggcgc gttcggtacg tttaccgtaa cacacgacat caccaaatac acccgcgcca
     2221 aaattttcag cgaagtcggc aaaaaaaccg agatgttcgc ccgtttcacc accgtggcag
     2281 gcgagcgcgg cgcagccgat gcggaacgcg acatccgcgg ttttgcgttg aaattctata
     2341 ccgaagaagg caactgggat gtggtcggca acaacacgcc cgtgttcttc ctgcgcgacc
     2401 cgcgcaaatt ccccgacctg aacaaagccg tcaaacgcga tccgcgcacc aatatgcgtt
     2461 ccgccacaaa caactgggac ttctggacgc tgctgcccga agccctgcac caagttacca
     2521 tcgtgatgag cgaccgcggt attcccgcca gctaccgcca tatgcacggc ttcggttcgc
     2581 atacctacag cttctggaac gaagcgggcg aacgtttttg ggtgaaattc catttccgca
     2641 gccagcaagg gattaaaaac ctgaccaacg aagaagctgc aaaaatcatc gccgacgacc
     2701 gcgaaagcca tcagcgcgac ctgtacgaag ccatcgagcg cggcgagttc ccgaaatgga
     2761 cgatgtacat ccaagtcatg cctgaagccg atgccgaaaa agtgccttat catccgtttg
     2821 acttgaccaa agtttggccg aaaaaagact atccgctgat tgaagtgggc gaattcgagt
     2881 tgaaccgcaa tcccgaaaac ttcttcgccg atgtggaaca atccgccttc gcaccgagca
     2941 acctcgttcc cggtatcggc gcgagcccgg ataaaatgtt gcaagcccgt ttgttcaatt
     3001 acgccgacgc gcaacgttac cgcttgggtg tgaacttccg ccaaatcccc gtcaatcgtc
     3061 cgcgttgccc tgttcacagc aaccagcgcg acggacaagg ccgcgccgac ggcaactacg
     3121 gcagcctgcc gcactacgaa cccaacagct tcggccaatg gcagcaacaa cccgacttcg
     3181 ccgaaccgcc tttgaaaatc aacggcgacg cggcacactg ggactaccgc caagacgatg
     3241 acgactactt cagccaaccg cgcgccttgt tcaacctgat gaacgacgcg cagaaacagg
     3301 ctttgtttga caacaccgcc gcagccatgg gcgatgcgcc cgacttcatc aaataccgcc
     3361 atatccgcaa ctgctaccgt tgcgacccgg catacggtga aggcgtagcc aaagccctcg
     3421 gactgaccgt cgaagacgca caagccgccc gcgcgaccga tcccgcactg ggtcaggccg
     3481 gtttgctgta agggggcatt atgtggatgg aaattgaaga aatcctgtcc cgcgccgtcc
     3541 gataccggaa ataaccgggc ataaaaatgc cgtctgaaac gtcgtttgac cgtttcagac
     3601 ggcatttcca cacctcgccc cgccgtttca gtgggcgttt tttattatag tggattaaat
     3661 ttaaaccggt acggcgttgc ctcgccttgt cctgattttt gttaatccac tataaacgca
     3721 aaatatcccg tcattcccgc accatcccca ccggttcaaa ccggcacgaa aacttttccg
     3781 cgtcattccc gcgaaagtgg gaatccggaa cgtaaaatct aaagaaaccg ttttatccga
     3841 taagtttccg tgccgacggg gctggattcc cactttcgtg ggaatgacgg gatttctgtt
     3901 tttgattttt tgtttttgcg ggaataacgg gatgcgggtt ttcgtgcggc gtttttcgcg
     3961 ctttgctgtt ttgttataat ccgccctttt taaggatggg tgcggtatga gtctttacgc
     4021 tttgctcttg gttgcgctgg gaatgtcgat ggatgcgttt gccgtcgcgc tggcaaaggg
     4081 cgcggcggtc aggatgcccc cgcgcaaaat tgcagcaacg gctttggtgt tcggtacggt
     4141 tgaagcgttc atgccgctgg cgggctgggt aggcggtttt tatgccaagc cgtttatcag
     4201 cgaatgggac cattgggtgg cttttgtcct gttgggcgga ctgggtctga aaatgatgcg
     4261 cgaagggttg tccggcgagg cggaagatgt gcgcgaaagc aaacaggaaa gcctatggat
     4321 gacggttttg actgcttttg gaaccagtat tgattccatg atcgtcgggg tgggcttggc
     4381 gtttatggag gtaaacatcg cctttgccgc cgccgtaatc ggtatggcgg ctacggtaat
     4441 ggttaccatc gggctgacgg cgggaaaggc ttttggcgta ttgttcggca ggcgtgcgga
     4501 atttgccgga ggtttggtgt tgattgccat cggtacatgg acgctcttat cgcatttggg
     4561 tttgattcaa tgatgtcgga aaataaaatg ccgtctgaag ctttaccggt ttcagacggc
     4621 attttgttaa acttgattct ctatcggtat tattgtgtaa agctattgaa aataaaatat
     4681 tgattttccg caaagatggc taaaggtgcg ataggtacac tgaagaaata atcggcagaa
     4741 caggtttgcc tgccggtttg aacaataatt ttaacctccc ataaggaata cacgatgtct
     4801 ttcaaactcc gttacctcgc atcagtattg gcattgtctt cactgttggc ggcatgcggc
     4861 ggtcaggaaa agtctgcggc aggcgatgct tctcctgctt ccgagaccga agcggcttcc
     4921 caagtacagg cctcggaagc cgttccttcc gcttcatcgg cctcgcccga agaccaagac
     4981 cttttgaaac gcgcgcaagg tgtattccaa cctttgccga ctgtcgaaga aatgcagaaa
     5041 atccgtccgt ttaccgaaga acaggtcaaa ctcggacacc aactctggta tgaaccacgc
     5101 ctttccaaag gcaataccgt aagctgcaac tcttgccaca accttgcttc cgccggtgtg
     5161 gacaatatgc cgaccagtca ggggcacaaa gggcagttcg gcggacgcaa ctcgcctacc
     5221 gcattgaatg ctgcgctgct gggcagccag ttttgggacg gacgtgccgc cgatgttgaa
     5281 gaacaggctg gcgggccttt ggtgaatccg gtggaaatgg cgaatgattc gcaagaggcg
     5341 gctgcagcca aaatcgccaa agttcccgaa tatcaagaaa tgtttaaaaa agcttttcct
     5401 gaagacggcg cggtttcgtt taaaaacatc actaccgcat tgggtgcgtt tgagcgtacc
     5461 ctgctgacgc cgaccaaatg ggacgaatac ctcaaaggca acgtcaacgc cctgagcgaa
     5521 caggaacgaa aaggcgtgcg cgcgttcatg gacaacggct gtattgcctg ccacaacggt
     5581 gtcaaccttg gaggcacgac cttccagaaa ttcggtctgg tccaagggcc gtattggaaa
     5641 ttcattgaag atccgaaacg cgacaaaggc cgtgctgacg taaccaaaaa aaccgaagac
     5701 gaatttttct tccgtgttcc ggggttgcgt aacgtggcta aaacttatcc gtatttccac
     5761 aacggcagcg tgtgggagct ggataaggca gttaccatca tgggtaaggc gcaattgggt
     5821 aaagacattc cgaaagaaga tgtggataac atcgtcgtat tcctgaatgc actttccggc
     5881 aatgtttccg aatcagcgcg cacgatgccg gaactgcccc tgaccgcacc gatggaatct
     5941 aagccggaca acaaataatc cggccggtac gcaaaaatgc cgtctgaaac gggaatctgt
     6001 ttcagacggc attttatatt tcgtcgcgtc agatacgcag ggtatgccgt agggtgtgct
     6061 tcagccagcc gtttccacca attcaaccaa ccatgtccgg gtcttatgcg tttttcacga
     6121 atgcgcggcg taggcggagg gattggtggg ctgaagccta attcaatggc tgcgttgtgt
     6181 aagccatgat gcgtttggat aattaatcaa tattcttgcg ctaccattac catcggcttt
     6241 gggggtttgt acttcggcat tatgataatc aatagcctgt ttggattgta ttcccgaatc
     6301 cgacagtttt agattataaa aaggccgtct gaaaaatttt cagacggcct tttatagtaa
     6361 atttcaaata aaaatatctt tgtcgtcatt cccacgcagg cgggaatcca gacttgtcgg
     6421 cacagaaact tatcgggtaa aacggtttct tcaattttat gttccggatt cccgcctgcg
     6481 cggggataac gattcaggta tttctacatc gaatccgctt tttatatcaa cctgactatc
     6541 aagccgcgtt gtcgaagccg ctgtggcgca gcagtgcgtc tatgctcggt tcgcgtccgc
     6601 ggaaggcttt gaaggattcc gccgcgctgc gggagccgcc gacggcaagg atttcttgcc
     6661 agaagcgttt gcctgtggcg gcgacgtcgt cgctttcttc aaaggcggcg taggcatcgg
     6721 tgctgaggac ttcggcccat gcgtagctgt aatagcctgc ggaatagccg ccggcgaaga
     6781 tgtggccgaa gctgttggcg aagcggttgt attcgggcgg ttggatgacg gcgacttctt
     6841 tgcgcacgct gtctaaaacc tgctgccagt ttttcagacg gcattcgtcg ctttcactgt
     6901 aaatcatcat atcgaagagg gcgaactcca tttgccggac gaggaacata ccgcgctgga
     6961 agtttttggc ggcgagcatt ttgtcgaaga gttctttcgg caggggctcg ccggtttctt
     7021 cgtgggcgga catttgtgcc aatacattgt attcccaaac gaagttttcc ataaactggc
     7081 tgggcagttc gaccgcgtcc cattctacgc cgttgatgcc ggacacgccc agttcgtcca
     7141 cttgggtaag caggtggtgc agtccgtggc cggtttcgtg gaagagggtg aggatttcgt
     7201 cgtggcttaa acgcgcttct ttgccgccga cgggcggggc gaagttgcag acgaggtagg
     7261 cggtgggcag ttgcagcgtg ccgtcggcaa agcggcggcg gcctttgtag tcgttcatcc
     7321 acgcgccgcc gcgtttgcct tcgcgtgcgt acaaatccat ataaacgccg ccgatggttt
     7381 cgccgttttg ttgcaattca aaatagcgcc cgtctttgtg ccagacgggg acggtttttt
     7441 cggcaaatcc gatgccgtag agttttttga tttgggcgaa cagtccgttt aatactttgc
     7501 cgacggggaa gtattttttg acttcggttt cgctgaatgc gtatttggct tcgcgcagtt
     7561 tttcgccggc gtagctcaag tcccacggct gcgggtcggc gagaccgagg tgttcgcggg
     7621 cgaaggcttt gacttcggcg aggtcttttt cggcgtaggg tttggcgcgg cgggcgaggt
     7681 cgtgcaggaa gtttaaaacc tgttcgggcg tgtccgccat tttggttgcc agcgacaatt
     7741 cggcgtaatt tttaaagccg agcagtttgg cggttttcaa tgcgttttcg agcgtgcggt
     7801 cgatgttggc ggtgttgtcg aatttgccgt cgtttgaaag ttcgctggca cgggtaacgt
     7861 aggcgcggta gatttgttcg cgcagttcgc ggttgccggc gtattggatg acggcaaggt
     7921 agtgcggaat ctgcaagccg attttgtaac ctgttttgcc ttcgctttgc gcggcggcgg
     7981 caaacatcgc gagcgcgtct tcgggaatgc cggcaagcgg tgcggcatcg tcaaagtaaa
     8041 tgccgaacgc gtcggtcgcg tctaggacgt tttgggagaa tttggcggaa agttgcgcgc
     8101 cttcggtttg cagttttgcc agttctgcct gctgttcggg cggcagttcc gcgccgctca
     8161 atacgaaatc gcgcaggtcg tgatcgagct tggttttttg tgcgggggaa agcgttgcaa
     8221 attcggggga atttttgatg gttttgaagc ggttgtacag ttcgatgtct tgtccgattt
     8281 cggtgaagaa gacggtgatt tcaggcatca gttcgttata gacggcgcgc agttcgggcg
     8341 tgtcgacgac ggagttgaga tgggacacga cgccccaaat cctgccgacg cgttcggtga
     8401 tgccggtcag acgctcgacg gtgttcgccc agccggtgtg cgtttgcgct ttgacggcgg
     8461 cgatttgtcc gcgcgcttcg gcgatggcgg tttggacggc gggtttgatg tcttcggttt
     8521 tgatttgatt aaaacggggt tcttcgccca agtggagcag tgcgttgtca atcataagat
     8581 gggtttcctt tgcgtgttgt gcttcagacg gcatatcggc aatatgccgt ctgaagccag
     8641 aaatggggcc ggccggggaa agttaaagtg tttcggagcc ggcgggatga tttaacaggc
     8701 ggtattgtac gcgcaaaaat tcgtccataa ccgcctgttg gatttccagc cgctttgaaa
     8761 cgggggaggc gaagcggacg atgatgcggt atgccttgtc gtcgtacggt acgcgggtaa
     8821 cgcgcggcct ggcggcgggc gtgataaaca gtttttccgc ctgcacgttt tccaaatacc
     8881 gctgaatggc ggggatgtag ggcgcgcaca agggctcgag tacggctttc agacggcata
     8941 cggcttcatc cgaatccaaa tggatgggaa cggggatttc gaccgtatgg atgacatagt
     9001 cgcccaaaat attgtcgcgg cgcacggggt ggctcaacaa caggctgttg gggaaagaaa
     9061 cggtggttcc cgcaagctgt ccgaccaagg ggttcggacc gacctgcatc atcagcgtgt
     9121 tcaacagatt gatgtcgacc acgcgcccgc gcaggccgtt gatttcgata tagtcgccga
     9181 ccgagtattg ctgggtggca gaccttaaaa tactgcccga cagacacata atcagttctt
     9241 ttgtcgccac gacgaccgcc gccgccaccg caaacatcga caaagccagt gtttgaattt
     9301 gcgccgacca gataaatgcc agcgaaaaca gcaccaaaag cagcgttata ttgcggctgg
     9361 caaccaaaaa ccgccgcttg ctttcgatgc cgaaatccgg atgccgtctg aagcggatgt
     9421 tcaacagaag ggcgcgcgcc agcagcaaag ccgcgaccgc cgccacggat tcggccgcct
     9481 ccgcgcgtat cgggacggca tcgggccaag tgttcaacat attccatatt tccatttccg
     9541 cgcccctgtc cgaaaagtat agggtgtaga ttttagtggc aaaaaaacgc ttttgccact
     9601 ttgccggcaa attccgcgcc cgtaccggcc gcaaatgcgc cgcacgccct ttcagacggc
     9661 atccggatgc ggataacgct gaatcaggcg gcgaaatcgg ctataataca caattatatg
     9721 cgccgtcccg cccgcttttt ccacaaggtg cggactgttt tggcggcatc gcaaaatctt
     9781 ttcaaaatcc ggcaaaaaat atgactgaac aaaaacacga agaatacggc gccgacagca
     9841 tccaggtgct cgaaggcttg gaagcggtac gcaaacgccc cggcatgtac atcggcgaca
     9901 cgcaggacgg cagcgggctg caccatatgg tgtttgaagt attggacaac gccatcgacg
     9961 aagcactcgc cggacattgc gacaaaatca cggtaacgat acacgccgac cattccgtca
    10021 gcgtcgccga caacgggcgc ggtatgccca ccggcatcca cccgaaagaa gggcgttccg
    10081 ccgccgaagt catcatgacc gtcttgcacg cgggcggcaa attcgacaac aacagctaca
    10141 aaatctccgg cggcctgcac ggcgtgggcg tatccgtcgt caacgcgctg tccgactggg
    10201 taacgctgac catctaccgc gacggcaaag aacacttcgt ccgcttcgta cgcggcgaaa
    10261 ccgaagagcc gctgaaaatt gtcggcgatt ccgacaaaaa aggcacgacc gtgcgcttcc
    10321 tcgccggcac ggaaaccttc ggcaatatcg aatacagctt cgacatcctc gccaaacgta
    10381 ttcgcgaact ttcgttccta aacaacggcg tggacatcga attgaccgac gagcgcgacg
    10441 gcaagcacga aagcttcgcc ctttccggcg gcgtggcggg cttcgtgcaa tacatgaacc
    10501 gcaaaaaaac gcccttgcac gaaaaaatct tctatgcgtt cggcgagaaa gacggcatga
    10561 gcgtcgaatg cgcaatgcaa tggaacgaca gctatcagga aagcgtgcag tgcttcacca
    10621 acaacatccc tcagcgcgac ggcggtacgc acctgaccgc gctgcgccaa gtgatgacgc
    10681 gcaccatcaa cagctacatc gaagctaacg aagtcgccaa aaaagccaaa gtggaaaccg
    10741 ccggcgacga tatgcgcgaa ggtttgacct gcgtgttgtc cgtcaaactg cccgacccca
    10801 aattctcatc ccaaaccaaa gacaaactgg tttccggcga aatcggcccc gttgtcaacg
    10861 aagtcatcaa ccaagcacta accgacttcc tcgaagaaaa tccgaacgaa gccaaaatca
    10921 tcaccggcaa aatcgtcgat gccgcccgcg cacgcgaagc cgcccgcaaa gcccgcgaaa
    10981 tcacccgccg caaaggcgtg atggacggct tgggactgcc cggcaaactc gccgactgcc
    11041 aagaaaaaga ccctgccctg tctgaactct acctcgtcga gggcgactcc gcaggcggtt
    11101 ccgccatgca gggccgcgac cgcaaattcc aagcgatttt gccgctcaaa ggtaaaattt
    11161 tgaacgtcga aaaagcacgt tttgaaaaaa tgctcgccag ccaagaggtc gccaccctga
    11221 ttaccgcgct gggtgcaggc atcggcaaag aagagttcaa ccctgaaaaa ctacgctacc
    11281 accgcatcat catcatgacc gatgccgacg tggacggtgc gcacatccgc accctgctcc
    11341 tgaccttctt ctaccgccaa atgcccgaac tggtcgagcg cggctacatt tacatcgccc
    11401 agccgccgct ctacaaagcc aaatacggca agcaggagcg ttacctcaaa gacgaactgg
    11461 aaaaagacca atggctgctc ggccttgcct tggaaaaagc caaaatcgtt tcagacggcc
    11521 gcaccatcga aggcgcagaa cttgccgaca ccgccaaaca attcttgttg gcgaaaaccg
    11581 tcatcgaaca ggaaagccgc ttcgtggacg aactcgtcct gcgtgccatg ctgcacgcgt
    11641 cgcccattga tttgacgtcg tctgaaaacg ccgataaagc cgttgccgaa ctttccggtt
    11701 tgcttgacga aaaagaagcc gccctcgaac gcatcgaagg tcatgaagga caccggttca
    11761 tcaaaatcac gcgcaagctg cacggcaacg tcatggtcag ctacatcgaa cccaagttcc
    11821 tcaacagcaa agcctaccaa accctcaccc aaaccgccgc cgcgctcaaa ggcttggtcg
    11881 gcgagggcgc caagctctac aaaggcgaga acgagtacga cgcggacagc tttgaaaccg
    11941 ctttggacat cttgatgagc gttgcccaaa aaggtatgtc catccaacga tacaaaggtt
    12001 tgggcgagat gaaccccgag cagctttggg aaaccacgat ggatcccgcc gtgcgccgcc
    12061 tgttgaaagt gcgcatcgaa gatgccattg ccgccgacga agtgttcgtt accctgatgg
    12121 gcgacgaggt cgaaccgcgc cgcgccttta tcgaaaacaa tgcgctgatt gcgcaaaata
    12181 tcgacgcata agtgccgttt taaaaaagga gacgggcagc ttgccgcgtc tccttttggt
    12241 ttgtcaaacg gaagccatgc cgtctgaaac cgcctttcgg agcaaaacca tgatcagcat
    12301 ttttgatatt ttcaaaatcg gtatcggccc ttccagttcg catacggtcg gcccgatgaa
    12361 ggccgccgcc gcctttgccg caggcttgga cgcgcaaacc gcccgcatcg ccatcgacat
    12421 ttacggctcg cttgccctga ccggatacgg acacggcaca tttgacgcgc tgatgctcgg
    12481 tttggaaggc agcctgccgc acgacatccc gcttgccggc attcccgaac gcctcggacg
    12541 catccgcacg cagcacatcc tccggctcaa cgggcaagaa atccgcttca tccccgaccg
    12601 cgacctgaac atacgcggca atcaagtgct gcccaaacac cccaacggcc tgcgatttac
    12661 cgcctatgct tcagacggca cggtattgaa ggaacaggtt tattattcgg tcggcggcgg
    12721 ctttgtcgtt accgaagaag acttcgagtg gcagtcggaa acggaaaaag ccgttcctta
    12781 tccttacaac agttgtgccg ggctgcttgc ccgatgccgt ctgaaccggc tcgacatctc
    12841 cgaagtcgtg ttggcaaacg aagccgcgct tgccggatgc ggcgaagccg aaatccgccg
    12901 ccgggttgcc ggtgttgccg agattatgga agagtgcatc aaacgcggct tgggtgcgga
    12961 cggcgaactg cccggcggat tgaacgtccg ccgccgcgcc ccgcagcttg ccgccaaact
    13021 caaagtcctg cgcgaaaccg aaatcgtcaa cacccagctc tggccgatgg tgtacgccat
    13081 ggcggtcaac gaagaaaacg ccgccggcgg acgcgtcgtt accgccccga ccaacggcgc
    13141 ggcaggcatc attcccgccg tattgcatta tttccgcaag ttcaaccccc acgccacaca
    13201 ggaacgtgtc gaaaacttcc tgctcaccgc aggcgcaatc ggcatcctct acaaaaccaa
    13261 cgcctccatt tccggcgcgg atgtcggttg tcagggcgaa gtcggcgtag cgtgttcaat
    13321 ggcggcgggt gcatacgccg aagtcatcgg cggcacgccc aaacaagtgg aaaacgccgc
    13381 cgaaatggca atggaacacc atttgggctt aacttgcgac cccgtcggcg ggttggtgca
    13441 aatcccctgc atcgaacgca acggcatcgc cgccgaaaaa gccctcaaac tcggcacgct
    13501 cgcgctcttg gaagacggca cggacaaaaa agtctcgctc gacgaagtca tccgaaccat
    13561 gctgcaaacc ggccgggaca tgaaggcgac ctacaaagaa acctcgcttg ccggactcgc
    13621 cgccacgctc cggaaaaaag ccgtccccgt atccgtgcgc gtggtcgagt gctgaggatg
    13681 gacagaaacg aaaatgccgt ctgaaaacag tttttcagac ggcatttcat ttattttttg
    13741 cgggtttacc gcttcaacgc caacgtcaac acgcccgccg taaccagccc caagcctatc
    13801 cattcctgcg tgttcgggcg ttcgtccaag aaaaccaccg ccatcaggaa tgcaaaccgg
    13861 cgttgtttgc agcatccggc aagaattttt atagtggatt aacaaaaatc aggacaaggc
    13921 gacgaagccg caggcagtac aaatagtacg gaaccgattc acttggtgct tcagcacctt
    13981 agagaatcgt tctctttgag ctaaggcgag gcaacgccgt accggttttt gttaatccac
    14041 tataaaaggt cttttctaaa atatgcagat attcgaatgt cgaatttgcc ttatgattac
    14101 ggttttctgt cttatccgcc ttaaaacgcc ggtattccgt ttgaaccaaa ttatatttgc
    14161 cgaaacactc aaaaatctcc cttacctgcc cgacagacat caagacttcg ttgttgtaac
    14221 tgagaaaaat atattgaaag tccgcatccc taatcaaagc ctcaaatacc ggaaccacat
    14281 ccgattttga tcaagtccgc gctaaaacac atcgtacagc gcaaaaccgg atgggaatgg
    14341 caaaatccgg aaacaaacat ttttcaaagc gtacaaacaa aaaataccaa ccggaggttg
    14401 gtatttctta act
//
LOCUS       DAXFHF010000055        14248 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXFHF010000055 DAXFHF010000000
VERSION     DAXFHF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.055"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(33..1133)
                     /locus_tag="ACFIRM_001590"
     CDS             complement(33..1133)
                     /locus_tag="ACFIRM_001590"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC3029185.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLRSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1458..3032
                     /locus_tag="ACFIRM_001591"
     CDS             1458..3032
                     /locus_tag="ACFIRM_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC3029186.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            3046..3234
                     /locus_tag="ACFIRM_001592"
     CDS             3046..3234
                     /locus_tag="ACFIRM_001592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029187.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(3272..4693)
                     /gene="hemN"
                     /locus_tag="ACFIRM_001593"
     CDS             complement(3272..4693)
                     /gene="hemN"
                     /locus_tag="ACFIRM_001593"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC3029188.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            4828..5562
                     /gene="fnr"
                     /locus_tag="ACFIRM_001594"
     CDS             4828..5562
                     /gene="fnr"
                     /locus_tag="ACFIRM_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC3029189.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(5675..6625)
                     /locus_tag="ACFIRM_001595"
     CDS             complement(5675..6625)
                     /locus_tag="ACFIRM_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC3029190.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            6990..7700
                     /gene="rmpM"
                     /locus_tag="ACFIRM_001596"
     CDS             6990..7700
                     /gene="rmpM"
                     /locus_tag="ACFIRM_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC3029191.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            8475..9431
                     /gene="thiL"
                     /locus_tag="ACFIRM_001597"
     CDS             8475..9431
                     /gene="thiL"
                     /locus_tag="ACFIRM_001597"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC3029192.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            9424..9909
                     /locus_tag="ACFIRM_001598"
     CDS             9424..9909
                     /locus_tag="ACFIRM_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC3029193.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAAFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(9969..11645)
                     /gene="ettA"
                     /locus_tag="ACFIRM_001599"
     CDS             complement(9969..11645)
                     /gene="ettA"
                     /locus_tag="ACFIRM_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC3029194.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            11881..13235
                     /locus_tag="ACFIRM_001600"
                     /pseudo
     CDS             11881..13235
                     /locus_tag="ACFIRM_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            13239..14200
                     /locus_tag="ACFIRM_001601"
                     /pseudo
     CDS             13239..14200
                     /locus_tag="ACFIRM_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
BASE COUNT         2981 a         3592 c         4043 g         3632 t
ORIGIN      
        1 cggcggaaat ggctttcaga cggtatttgc gctcaataat aatatcccgc gcccagaata
       61 cacggtttgg atgcgccggt tgctttgtgc ggactaccgg ggatgcggtt aatccaacac
      121 gccgccaacc acgcaaatgc ggcggcttcc acccattgcg gatcgaggtt caggtcggcg
      181 gtgctgcgta aggaaacgcg tgtgccgaaa cattctgcca aatccgccat taaaacagga
      241 ttgcggatgc cgccgccgca aatgtacatt tgacgggcat ctgccgctgc gtgtgagacg
      301 gcgtcgcaaa cggtttgcgc ggtgaatcgg gaaagcgtcc gtaatacgtc gtatcggttt
      361 tcgccgccgt caaggtaggt ttcgagccaa tttagggcaa acagttcgcg ccccgtgctt
      421 tttgggtggg gttgtgagaa atacgggtgg gcgagcagcc tgccgagcag ttgcggcaat
      481 atgttgcctt gtgccgcctt tgcaccgttt ttgtcgtaag gcagctgcca gtgtgcctgc
      541 gtccacgcgt ccatcagcat attgcccggc cctgtgtcga agccgaaggc gggtgcgccg
      601 ggggggagta cgctgatgtt ggcaatcccg ccgatgttca gtaccacgcg tgtttccctg
      661 tcatcgcgga acagggcttc gtgaaaggcg gggacgagcg gcgcaccttg tccgccggcg
      721 gcaaggtcgc ggctgcggaa gtcgccgacg gtaaaaatcc gcgtcagttc cgccagcagc
      781 ggcaaatcgg caagctgtat gctgtaaccg tgttccggcg cgtgtcggac ggtttgcccg
      841 tggcagccga gggcggtaat gtcgcacgga gcgaggtttt gactgcacag cagttcggcg
      901 gcggtttgcg cgtacaggcg gctgagttct tgcgacaaca tcctgctgcg gtgcagttcg
      961 tctgtgcctg tgtcctgcaa atccagcaat ttgcggcgca accggtcagg gtagggggta
     1021 aaggcgtgcc cttccgcgcc cagccatttg ccgccgtcca tccgtaccag cacggcatcc
     1081 gccccgtcca tactggttcc cgacataatg ccgatgtaaa gctgtgtttc catcatcact
     1141 cccaaactgg tgcaaaacgc cattttaacg tgtattgacg ctcgtatacc gatttgccgc
     1201 cgcagtgtaa ataaagtgta aataaatgtt ttaagacggg tggaaaaata ttataatgcg
     1261 cccgcaacat ccagtagtag aagtgtcata caaaccgttt ccggcagcag ttttgcattc
     1321 ggtcaggttt gggggtattc ggatgcggtt cagaaggaag gatgcgtctg ccatatcccg
     1381 aaacggcagt tcgaccggag gcagcaatac agtgtcggca acactcatga tttccaccac
     1441 attaaaggaa gattgccatg gctcaaatcc aaatgagcgc aaatgttaaa accatcaacg
     1501 ccgtctttgc cgccatgctg gtaggtacag tcggctattt tatttattgg ggcttgggtt
     1561 atacccatta caattacgcc gccttattca ttattgccac gatgttcggt gtgtttatgg
     1621 cgttcaacat cggcggcaac gatgttgcca attctttcgg caccagcgtc ggtgcaggca
     1681 cgctgaccat cccgcaggct ttgctgattg cggcggtatt tgaggtcagc ggcgcggtca
     1741 tcgcgggcgg cgaggtaacc gataccatac gcaaaggcat cgtcgatttg aagggtgttg
     1801 atttcgaacc catacagttt gtgtttatta tgatgtccgc gcttttggcg gcggcgttgt
     1861 ggctgctgtt cgcgtcccgc aaaggcctgc cggtttcgac gacgcacgcg attatcggcg
     1921 gcatcgtcgg cagtgcgttg tgtatggcgt ttacgcataa tgcggacggg gtggcgttga
     1981 tacggtgggg caagctgggc gaaatcggga tgtcttgggt attgtcgccc gtgttgggcg
     2041 gcgcggtgtc ctatttcctg ttttcgcgcg tcaagaaaaa cgtcttagat tacaacgctt
     2101 gggcggaagg aacgctcaag ggcatcaagc aggaaaaaaa ggcctataaa gaacggcacc
     2161 gcctgttttt cgagggtttg tccgaagccg aaaaagtcga gtacgccacc aaaatggcgc
     2221 acgacgcgca aatttacgac gaacccgaat tcgatcctca agagctgcaa tcggagtatt
     2281 accgcggtct ttatgcgttc gacaaccgta aaaacaatgt cgattcctac aaggcgctgc
     2341 attcttggat tccctttatc gcttcgttcg gcacgatgat gatttccgct atgctgattt
     2401 tcaagggctt gaaaaacctg catttgggga tgagcaacgt caacagcttc ctgaccatct
     2461 ttatgatagg cgcggcggtg tggatgggga cgtttgtgtt tgccaaaagc ctcaagcgta
     2521 aagacttggg caaatcgacc tttcagatgt tttcatggat gcaggtcttt accgcctgcg
     2581 gcttcgcatt cagccacggt gcgaacgata tcgccaacgc catcggcccg tttgccgcga
     2641 ttatggatgt tttgcggacg aacagcgttg ccgcgcaaag cgccgtccct ccgattgcga
     2701 tgctgacttt cggcatcgcg ctgattgtcg gtttgtggtt tgtcggtaaa gaggtgatta
     2761 aaaccgtcgg tacgagtttg gcggaaatgc atcctgcttc gggttttacc gccgaactgt
     2821 ccgccgcatc cgtcgtgatg ggcgcgtcgc tgatggggct gcccgtgtcc agtacgcata
     2881 tcttggtcgg cgcggtactc ggtatcggtc tggtcaaccg caatgccaac tggaaactga
     2941 tgaaacccat cggtttggcg tgggtcatta ccctgcctgc cgccgctgta ttgtcgatgg
     3001 ccagctattt gattttgcag gcagtgttct gattgtaaaa tactgatgcc gtctgaaccc
     3061 gtgttcagac ggcattttgt tgatggaatg tgcgggcttg tgccttatgc acaatctgtt
     3121 ctgtcgggat atgctgtttg gtatcttggt ttcggaacgg tcggacacaa aggttatgat
     3181 atgccgccgc ctgttcttga aaacacttat cctcggcagc aaaatgccgt ctgaaaaagc
     3241 ctttcagacg gcttttgtac gttagccgca atcacaccgt ttgcgaatat ttcgctttgg
     3301 tttctttgtg gcgcaggtgg tagtcgaaga ccatggcgat gttgcggatg aggaagcgtc
     3361 ctttcggtgt aacggtcagc ccgtggctgt tcaggcgcac caatcccaaa ccggcgagtt
     3421 tttccaaatc cgccagttcg tctttgaagt agcggtcgaa cgggatgccg aacatacttt
     3481 cgtaaatcct gtagtcgagc gcgaagcggc acattaaatc ctgaatgatg ttgcggcgca
     3541 ggatgtcgtc ctgattgagc tggtagccgc gcatgatggg cagtctgcct tcgtcgatgg
     3601 cggcatagta ggcatcgatg tcgcgttcgt tttgggaata ggtgctgccg attttgccga
     3661 tggacgacac gccgatggcg accaaatcgc aatccgcgta ggtcgaatag ccttggaagt
     3721 tgcgttggag gaagccttct ttgagggcga tggagagttc gtcgtcaggt ttggcgaaat
     3781 ggtccatgcc gatgaagacg tagccgcgct ctgtcagggt ttggacgcag tattgcagca
     3841 tatcgagctt ctcttcgctg tcggggacgg cggcggtatc gatgcggcgt tgcggtttga
     3901 acacgtgcgg caggtgggcg tagtggtaaa gggcgaggcg gtcgggatcg agcgacaaaa
     3961 cggtatctat ggtggttttg atgctttcgg aagtctgatg cggcaagccg taaatcaaat
     4021 cgacgctgac ggatttgaac cctgcctcac gggcggcatc gatgacttct ttggtttctt
     4081 cgtagctttg gatgcggttg actgccgcct gcactttggg gtcgaaatcc tgaatgccga
     4141 cgctcatgcg gttgaagccg agtctgccga gcatgaggac ggtgtcgcgg ctgactttgc
     4201 gcgggtcgat ttcgatggaa tattcgccgg acggtatcag ttcgaaatgt ttgcggatca
     4261 tgcggaagac acgttcgatc tgttcgtcgc tcaaaaaggt cggcgtgccg ccgccgaagt
     4321 gcagttgggc aagctggtgc cgtccgttca gatgtggagc gagcagttcc atttcttttt
     4381 caagatattc gatgtaggta tcggcgcggc ttttgtcttt ggtgatgatt ttgttgcagc
     4441 cgcagtagta gcagatggtg ttgcaaaacg gaatgtgaat gtaaagggaa agcggtttgt
     4501 ttaacgcgcc cataccgcgc aaatgtaaaa ctttgatata ttcgccttcg cggaaaccgt
     4561 catggaaacg gtcggcggta gggtaggaag tatagcgcgg gccgctggcg ggcaggctgg
     4621 caatcagcgc gcggtcaaac tcggggcggt catcgtttac attgtgattg ttctgtatct
     4681 gaatgatttt catggtgtgt gtgcggtttt atgatgttag tcaaattttg gatagtttgg
     4741 tagaatgcca cagtatgata aacctgtctt gatatgtgtc aacaagcaca taatcgggat
     4801 ggggcaaaac acacgaaaag gtacggtatg gcttcgcata atactacaca tcagatgaaa
     4861 acgctgtgtt cttcctgttc tttgcgggaa ctctgcctgc ctgtcgggct gctgcccaac
     4921 gagttcagcc aactcgatgc cgtcatccgc caaagccgcc gcctgaaaaa aggcgaatac
     4981 ctgttccgtg ccggcggagc ctttacctcg ctctttgcca tccgttcggg cttcttcaaa
     5041 acaaccgtcg ccagtcagga cggccgcgat caggtaacgg gtttctttat gtcgggcgaa
     5101 ctcatcggca tggacggcat ctgttcctat gtgcacagtt gcgacgcggt cgccttggaa
     5161 aacagcgagg tatgcgagtt gccgtttacc catatcgaag agctcggtca gaatatcccc
     5221 agcctgcgta cacacttttt ccgcatgata agccgcgaaa ttgtgcgcga tcaaggtgtt
     5281 atgctgttgt tgggcaatat gcgcgccgaa gaacggattg ccgccttcct gttgaacctt
     5341 tcccaacgcc tttattcccg aggtttcgcc gccaacgact tcatcttaag aatgtcccgc
     5401 gaagaaatcg gcagttatct cgggctgaaa cttgaaaccg tcagccgcac attatccaag
     5461 ttccatcagg aaggattgat ttccgtcgaa cacaaacaca tcaagattct caatctgcag
     5521 gtgctgaaaa aaatggtatc cggttgttcg cacgccattt gattaacctg tacgaacatt
     5581 tcagacggca ttctcaataa acacagggca gacgaaaaca tctgccctgt ttgttgtatc
     5641 tgccgcaaag tgccgcctga aaaccggcag ccgcctaaat cgaaaaatcc tcgctgatgg
     5701 gcgtgtacag aatcctatcc accttctcgc gtgtcaggtg cggcgcaaac gcttggataa
     5761 agtcgtaggc atatccgcgc aaataagtat cgctgcgcaa agctatccac gtcggcgacg
     5821 gctcgaacag gtgtgccgca tccacaagct gcaaatcgcc gtccgtatcc gggttgtatg
     5881 ccattttcgc catcagtccc acgcccaaac ccaagcgcac ataagtcttc aatacatccg
     5941 tatccgccgc cgccaatgcg acatccggcc gttccaaacg ggctttggcg aatgcccgcg
     6001 cgatgctgct gcccgcattg aatgcaaatt cataagtaat cagcggaaac ctcgccaaat
     6061 cttcaatacg gaggggattc ctgcattcga gcagcggatg gtcgttcggc acaatcaccg
     6121 cgtgtgtcca gtcgtagcag gaaagccttc ccaactcggg gtggtcgtct atccgttccg
     6181 taacaatcgc caaatcagat tctcccgaag taaccatttg ggcgatggcg gcagggcttc
     6241 cctgtttgat ggtcagattg actttcggat agcgtttcac aaaatcggca acaatcaacg
     6301 gtagggcata gcgcgcctgc gtatgcgtcg tggcaaccgt cagcgaaccg ctgtcctgtc
     6361 cggtaaactc gctgccgata tttttaatgt tctgtacatc gcgcaaaata cgttccgcaa
     6421 tatccaaaac caccttgccc ggctgcgaga ccgaaaccac gcgcttgccg ctgcggataa
     6481 aaatctgaat gccgatttct tcttccagca atttgatttg tttggagatg ccgggttgtg
     6541 atgtgaataa ggcttcggcc gcttcggaaa cgttcaggtt gtgccggtaa acttctaagg
     6601 cgtatttcaa ttgttgtaat ttcatggcgg gtcggtgtgg gtctgtgtcg ggtggctgaa
     6661 cattgtttat aatttatcat attttcttgc cggtacggta tggggctttg ccgttgtgtt
     6721 tgttgttttt gtgcaacggc aatcgtgcga tatggaaaaa aatcccccta aagtaatgac
     6781 acggaattga tttttcggca tgatagacta tcaggaaaca ggctgtttta cggttgtttt
     6841 caggcgttga gtattgacag tccgccccct gtttctttat agtggagact gaaatatccg
     6901 atttgccgcc atgtttctac agcggcctgt atgttggcaa ttcagcagtt gcttctgtat
     6961 ctgctgtaca aatctaatga gggaataaaa tgaccaaaca gctgaaatta agcgcattat
     7021 tcgttgcatt gctcgcttcc ggcactgctg ttgcgggcga ggcgtccgtt cagggttaca
     7081 ccgtaagcgg ccagtcgaac gaaatcgtac gcaacaacta tggagaatgc tggaaaaacg
     7141 cctactttga taaagcaagc caaggtcgcg tagaatgcgg cgatgcggtt gccgtccccg
     7201 agcccgaacc cgcgcctgtc gccgttgtgg agcaggctcc tcaatatgtt gatgaaacca
     7261 tttccctgtc tgccaaaacc ctgttcggtt tcgataagga ttcattgcgc gccgaagctc
     7321 aagacaacct gaaagtattg gcgcaacgcc tgagtcgaac caatgtccaa tctgtccgcg
     7381 tcgaaggcca taccgacttt atgggttctg aaaaatacaa tcaggctctg tccgaacgcc
     7441 gcgcatacgt agtggcaaac aacctggtca gcaacggcgt acctgcttct agaatttctg
     7501 ctgtcggctt gggcgaatct caagcgcaaa tgactcaagt ttgtcaagcc gaagttgcca
     7561 aactgggtgc gaaagcctct aaagccaaaa aacgtgaggc tctgattgca tgtatcgaac
     7621 ctgaccgccg cgtagatgtg aaaatccgca gcatcgtaac ccgtcaggtt gtgccggcac
     7681 gcaatcatca ccaacactaa ggctaggtaa tatcttgccg atgcatgagg ttagcggatt
     7741 ttgtaccggg tactgttgca atattcgtga aacgtcggcc ggtatcgatg atgtgaaaca
     7801 aaccccgctt ttgcggggtt tgtttttttg ggtggttttc tgaaacggct atcgtcagaa
     7861 tcggggtgca ggttcggatt cggattcaga ttcatgtttg tgtcccattg ccgcgcttta
     7921 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca atagtacggc
     7981 aaggcgaggc aacgccgtac cggtttaaat ttaatccact atatcggttg aaactctgat
     8041 tttaaggcgg taggatgtgg gtttgcccat agcaagggaa tcctttctgt atcaagcccc
     8101 gaaagggata attcatacaa attcacgcct ttccccctca ttgggaaatg gatggaatcg
     8161 tgcccgatgt gtgcggcact gtatgccgga tatggtttta tcatcatccc ttttcggttg
     8221 aaaccccgcc agttgcagcg attgagccta atcggtggcg gaagttgccg ctttgcattc
     8281 ggggcggcgt gcagtgcggt gctttgatat gccgtttgtg tgttgaaaca gggtggtcgg
     8341 tgcatacggg tacggtatgg ctaaagctaa aagtgaaata cgctgaaaca ctgaatgagc
     8401 cgctttattg tttgtacgac ctttgctgct ttgctatgat ttaaattgga ttcgcacgcc
     8461 ggatattttg ggatatgaaa gaattcgact tcatcaaacg gtatttgcaa acaggtacgg
     8521 ataaggatgt cgtattgggc ataggcgacg atgcggcgat tgtccgcccg cgcgaaggct
     8581 tcgatttgtg tttcagtgcg gatatgcttt tgaaggacag gcattttttt gcagatgtca
     8641 aacctgaagg cttggcttgg aaggttttgg ccgtcaatat ttcagatatg gcggcgatgg
     8701 gtgcgatacc gcgttgggtg ctgctgagcg cggcgttgcc cgaattggat gaagtatggc
     8761 tggaacggtt ttgcggcagc tttttcggtt tggcaaaaaa gtttggcgta acgttaatcg
     8821 gcggcgatac gaccaagggc gatatggcgt tcaatgtaac cattatcggc gaattgccga
     8881 agggtagggc gttgcggcgt gatgcggcgg ttgcgggcga cgatatttgg gtgtcggggc
     8941 gtgtcggtat ggcggcggcg gctttgaact gccgtctgaa acggtgtgtg ctgccggatg
     9001 atgtgttcgc cgaatgcgaa caaaagctgc tccgtcccga gccaagggtt gggctggggc
     9061 ttgcgctgtt gccgtttgcc cgggcggcac aggatgtttc agacggcctt gcgcaagatt
     9121 tggggcatat cctgaccgct tccggcgtgg gggcggaaat ttgggccgat tcgctgccat
     9181 ctttatctgt attgaaagat attttgcccc gagcgcaatg gctgtcttat actttggcgg
     9241 gcggcgacga ttacgagctg gtttttaccg ctccggaaag ttgccgcagc cgcgtgcttg
     9301 atgcggcgga acggtgcggc gtgccggtaa cgtgcatcgg caaaatcaac ggaggatgcc
     9361 gtctgaaggt tttagatgcc ggcggcaggg aattggaact acattcttta ggattcgatc
     9421 attttggctg attttaaacc tgactttgcg tggctgttga aacggccgtt gtgttttttg
     9481 gctttcggtt tcggcagcgg gctggctgcg tttgcgccgg gcacattcgg cactttggcg
     9541 gcactgcctt tggcgtttgt gctgattttg ctcggcatag acgggctgct gctggctttt
     9601 gtgtgtatcg tgctgtttat gtggggcata cgtatttgtg cgtgtgcgga acgtgaaacg
     9661 ggtgtcagcg accacggtgg gattgtttgg gacgagattg tcgccatgct gtttgtgctg
     9721 gcgtttgtgc cgttcaggtg gacgtggtgg ctggcggcat ttgttctgtt ccgtctgttt
     9781 gatgcgctca aaccgccccc cgtcggttgg tttgacaaga atctgcacgg cggtttgggc
     9841 attatggcgg acgatatggc ggctgcggtg atgaccttga ttgtcttgag gattgcaatg
     9901 ctgttttaaa cggggctgcc ttgtaaaaat gccgtctgaa agcctttcag acggcattgt
     9961 ttcggaggtt aacgcgttac cggtttgtat ttgatgcgtt tcggtttcgc gccttcttcg
    10021 ccgagtcggc gtttcttgtc ggcttcgtat tcttgatagt tgccgtcgaa gaacacccat
    10081 ttggagtcgc cttcacacgc caagatatgc gtggctatgc ggtcgagaaa ccagcggtcg
    10141 tgcgaaatca ccatcacgct gccggcaaat tccaacaatg cgtcttccaa cgcgcgcagg
    10201 gtttccacgt cgagatcgtt ggacggttcg tccagcagca acacattgcc gccgcccaac
    10261 aaggtttttg ccaagtgcag acggccgcgt tcgccgccgg aaagctgcct tgcgattttg
    10321 ctttggtcgc tgcctttaaa gttgaagcgt cccaaatatt ggcgggcggg gatttcaaac
    10381 tgtccgacct gcaaaatatc gcgaccttcg gcaatgttgt cgaacacggt tttgtcgttt
    10441 tgcaaacctt cgcggctttg gtcaatcaag ctcattttca cggtttgccc gattttcact
    10501 tcgcccgaat cgggctgctc tttgcccgca atcattttga acagcgtcga tttacccgcg
    10561 ccgttcgggc cgatgatgcc gacaatcgcg cccgccggca ctttgaagct caaatcgtca
    10621 atcagcactt tatcgccgaa cgatttggaa acattcacaa attcaatcac ttcgttaccc
    10681 aaacgctcgg caacagggat aaagatttcc tgagtttcgt tgcgtttttg gtattcgtag
    10741 ttgctcattt cttcaaaacg cgccaaacgc gctttgggct tggcttggcg gcctttggca
    10801 ttttggcgca cccattccaa ttcctgcttc atcgccttca cgcgcgcggc ttcggatttc
    10861 gcctcgtttt ccaagcgttt ttctttctgc tccagccaag acgagtaatt gcctttccac
    10921 ggaatgccgt gtccgcggtc gagttccaaa atccattcgg cggcgttgtc gaggaagtag
    10981 cggtcgtgcg ttaccgcgac cactgtgccg gggaagcgca cgaggaattg ctccagccat
    11041 tccaccgatt ccgcatccaa gtggttggtc ggctcgtcca agagcagcat atcgggcttg
    11101 ctcaacaaaa gtttgcacaa agcgacgcgg cgtttttcac cgcccgaaag attgccgatt
    11161 ttggcatccc aatcaggcag gcgcagcgcg tcggcagcga tttccaattc gtgttccgca
    11221 ccgccgccag ttgacgaacc cgccgcgata atcgcttcca agcggccttg ttcttccgcc
    11281 agcgcgtcaa aatccgcatc gggattggcg tactcggcat acacttcttc caaacgtttc
    11341 tgtgcggcag ccacttcgcc caaaccgctt tccacttcct cgcgcacggt tttttccgga
    11401 tcaagctccg gctcttgcgg cagatagccg attttgatgc cgcccatcgg cacggcttcg
    11461 ccctcaaatt ctttatccac gcccgccata atccgcagca cggtggactt gcccgtgccg
    11521 ttcaagccca agaggccgat tttcgcgccg gggaagaaag aaagggaaat atctttaatg
    11581 atggttttct gcggcggcac aaccttgctc acgcgcagca tagaatagac gtattgttgg
    11641 gacatagttt tctcgattcg gtcaaacaaa tttcagacgg ccattttaac cgataatttg
    11701 atttaagcca gtttatccgc gaaccggtat tgccaaaatc gggcaggatt cataaaatcc
    11761 gcctatccct ttgaaattat acagacaaaa aaataataat gataggggat cgccgcgccg
    11821 gcaaccattt cggattttcc aaagcaaata cttttcaaat caaaaaaaag gatttacttt
    11881 atgtcggaat atacgcctca aacagcaaaa caaggtttgc ccgcgccggc aaaaagcacg
    11941 atttggatgt tgagcttcgg ctatctcggc gttcagacgg cctttaccct gcaaagctcg
    12001 cagatgagcc gcatttttca aacgctaggc gcagacccgc acaatttggg ctggtttttc
    12061 atcctgccgc cgctggcggg gatgctggtt cagccgatag tggctactac tcagaccgca
    12121 cttggaagcc gcgcttgggc ggccgccgcc tgccgtatct gctttacggc acgctgattg
    12181 cggtcatcgt gatgattttg atgccgaact cgggcagctt cggtttcggc tatgcgtcgc
    12241 tggcggcctt gtcgttcggc gcgctgatga ttgcgctgtt ggacgtgtcg tcgaatatgg
    12301 cgatgcagcc gtttaagatg atggtcggcg atatggtcaa cgaggagcag aaaagctacg
    12361 cctacgggat tcaaagtttc ttagcgaata cggacgcggt tgtggcagcg attctgccgt
    12421 ttgtgttcgc gtatatcggt ttggcgaaca ctgccgagaa aggcgttgtg ccacaaaccg
    12481 tggtcgtagc attctatgtg ggtgcggcgt tactgattat taccagtgcg ttcacaatct
    12541 ccaaagtcaa agaatacgac ccggaaacct acgcccgtta ccacggcatc gatgtcgccg
    12601 cgaatcagga aaaagccaac tggttcgaac tcttaaaaac cgcgcctaaa gtgttttgga
    12661 cggttactcc ggtacagttt ttctgctggt tcgccttccg gtatatgtgg acttactcgg
    12721 caggcgcgat tgcagaaaac gtctggcaca ctaccgatgc gtcttccgta ggccatcagg
    12781 aggcgggcaa ccggtacggc gttttggcgg cggtgtagtc ggttgcggcg gtgatttgtt
    12841 cgtttattct ggcaaaagta ccgaataaat accataaggc gggttatttc ggctgtttgg
    12901 ctttgggcgc gctcggtttc ttctctatct tcttcatcta caatcaatac gcactcatcc
    12961 tgtcttatat cttaatcggc atcgcttggg cgggcattat cacttatccg ctgacgattg
    13021 tggccaacgc tttgtcgggc aaacacatgg atacttattt gggcctgttt aacggctctg
    13081 tctgtatgcc gcaaatcgtc gcttcgctgt tgagtttcgt gcttttcccg atgctgggcg
    13141 gccatcaggc aaccatgttc ttggttgcag gcgcagtctt gctgctggga gccttctcag
    13201 tctgtctgat taaagagacc cacggcgggg tttgagcgat gagcgatacc cccgctaccc
    13261 gcgatttcgg cctgatcgac gggcgggccg taaccggcta tgtgctgtcc aaccggcgtg
    13321 gtacgtgcgt cttcgtgctg gacttgggcg ggattgtgca ggaattttcc gttttggcag
    13381 acggcgtgcg cgaaaacccc gtggtgtcgt tcgacgatgc ggcttcctat gcggacaatc
    13441 cgtttcagat taacaagcag atagggcgcg tggccggacg catccgcggt gcggcgttcg
    13501 acatcaacgg taggacttac cgcgtggagg ccaacgaagg caggaacgcg ctgcacggcg
    13561 gttcgcacgg gctggccgtt acccgtttca acgcggtggc ggcagacggc cgacggttat
    13621 cccaacgatt tggatatttc ctaccgcttg gacgaggatg gccggcttac cgttacctat
    13681 cgcgccaccg cgctcggcga cacggtgttc gacccgacgc tgcacattta ctggcggctg
    13741 gacgcgggcc tgcacgatgc ggttctgcat attccgcagg gcggacatat tccggccgat
    13801 gccgaaaaac tgcccgtctt aacggtttca gacggcctcg aagtatttga tttcagccgg
    13861 cccaagccgc cggatgccgc cgttgccgcc ctgcgccgcg aaacgggccg gttttgacga
    13921 cgcttaccgc gtgccgtccg atataggccg tcccgccgca gtgttgcaag ccggacgccg
    13981 ccgtcgtatc agcatataca gcgaccgcaa cggcttggtc atctttaccg ccgccccgca
    14041 ggatttcgcg cggcacgatg cgggcgttta cgacgcgctg gcgaccgagg cgcagacgct
    14101 gcccgacagc ctgcattggc ccgaattcgg caatatccgt ctgaacaagg gtgatactag
    14161 ggaggcgacg attgcttacg gcatcgaatc cctttcttag gagcttccta acaccggttt
    14221 cagacgacct ttttatagtg gattaaca
//
LOCUS       DAXFHF010000056        14111 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXFHF010000056 DAXFHF010000000
VERSION     DAXFHF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.056"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            468..1577
                     /gene="fic"
                     /locus_tag="ACFIRM_001602"
     CDS             468..1577
                     /gene="fic"
                     /locus_tag="ACFIRM_001602"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC3029195.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     VRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2305..3117)
                     /gene="murI"
                     /locus_tag="ACFIRM_001603"
     CDS             complement(2305..3117)
                     /gene="murI"
                     /locus_tag="ACFIRM_001603"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC3029196.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3306..3767
                     /gene="tsaE"
                     /locus_tag="ACFIRM_001604"
     CDS             3306..3767
                     /gene="tsaE"
                     /locus_tag="ACFIRM_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC3029197.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            3764..5014
                     /locus_tag="ACFIRM_001605"
     CDS             3764..5014
                     /locus_tag="ACFIRM_001605"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC3029198.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5096..5914
                     /locus_tag="ACFIRM_001606"
     CDS             5096..5914
                     /locus_tag="ACFIRM_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3029199.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(5960..7102)
                     /locus_tag="ACFIRM_001607"
     CDS             complement(5960..7102)
                     /locus_tag="ACFIRM_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC3029200.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(7151..7573)
                     /locus_tag="ACFIRM_001608"
     CDS             complement(7151..7573)
                     /locus_tag="ACFIRM_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029201.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7566..8375)
                     /locus_tag="ACFIRM_001609"
     CDS             complement(7566..8375)
                     /locus_tag="ACFIRM_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3029202.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8457..8834)
                     /gene="acpS"
                     /locus_tag="ACFIRM_001610"
     CDS             complement(8457..8834)
                     /gene="acpS"
                     /locus_tag="ACFIRM_001610"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC3029203.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(8884..9612)
                     /gene="pdxJ"
                     /locus_tag="ACFIRM_001611"
     CDS             complement(8884..9612)
                     /gene="pdxJ"
                     /locus_tag="ACFIRM_001611"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC3029204.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAQ
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(9665..10408)
                     /gene="recO"
                     /locus_tag="ACFIRM_001612"
     CDS             complement(9665..10408)
                     /gene="recO"
                     /locus_tag="ACFIRM_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC3029205.1"
                     /translation="MSEHRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10445..11572)
                     /gene="pheA"
                     /locus_tag="ACFIRM_001613"
     CDS             complement(10445..11572)
                     /gene="pheA"
                     /locus_tag="ACFIRM_001613"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC3029206.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIRHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(11611..12839)
                     /locus_tag="ACFIRM_001614"
                     /pseudo
     CDS             complement(11611..12839)
                     /locus_tag="ACFIRM_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(12955..13830)
                     /locus_tag="ACFIRM_001615"
     CDS             complement(12955..13830)
                     /locus_tag="ACFIRM_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC3029207.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
BASE COUNT         3264 a         4040 c         3804 g         3003 t
ORIGIN      
        1 gtccggattc ccgcctgcgc gggaatgacg ggatttgagg tttctgtttt tgattttctg
       61 ttttcacggg aatgacggtt taaaagttac ccgaaacctc aaaaaaaagc tgtgttttaa
      121 tatagtgttg atagacgtac ttggcttcca tgtattcaat cgtggaaatc tatatcttcg
      181 tcctcgccga aatagtctat gcccgatata caattttgat acacaaactt ggaaatatgg
      241 gtatcgtcgc cggagcgata gaatgcggac agttatatat atacggtttt ttaggggagc
      301 gggcagatga agggcggatt ttgaattaac cccatgcaaa ttgacttttg cgggcggttt
      361 taccgcccat atatttacaa aagccaaatt tttaaacata tatccttgat atatacacgg
      421 cgtaaacata tactggaaac atctttaaat tttccgaaat tttaaacatg agcaactgga
      481 aacccgatat tccctataac gatttaccac ccctgccgcc aaaacaggat attgaaagca
      541 aaaccatcct gaaacgttgt atagccgccc gtgcatccct tgcccgttta aagcaggcgg
      601 cagaattgat accgaatcaa gccatgctga ttaacaccct tcctgttatg gaagcccgtg
      661 caagttcgga aattgaaaac atcgtaacca ccacggacaa gctgtttcaa tccctgcaaa
      721 tggatacgga acggcaagac cctgccacga aagaagccct gcaataccgc accgccctgt
      781 ttgcaggcta tgaatcactg gcgagccgcc ctttatgcac acaaaccgcc atcatggtct
      841 gcaacgccat caagcacccc tacgaaacgg ccatccgcaa aacaggcggc acagccctaa
      901 aaggaggtaa cagcggaaat gttgtctata ccccgcccga aggagaagaa accatacgcg
      961 gcaagctggc aaattgggag cggtttattc acgaaagcgg cgatttagac ccgcttgtca
     1021 tcatggcggc ggcacattac caatttgaag ccatccaccc gtttacggac ggcaacgggc
     1081 ggacgggacg catattgaac agcctgctat tgattgaaaa agggcttttg gatttgccta
     1141 ttttgtattt gagccgctac atcatcgaaa acagggcgga ctattaccgc ctgcttttag
     1201 gcgtaaccga acggcaggat tgggaaagct ggataatcta catcttagac ggcgtagccg
     1261 acaccgccga ttggacggta tcgaaaatag atgcggtacg ctgcctgttc gagcagacac
     1321 gtcaacacat acggacacac gcacaaggaa tctacacgca cgaactggta aatcttctgt
     1381 ttgagcagcc atatacacgc attgccaacc tagaagcggc agggatagcc aaacggcaga
     1441 cggcctctaa gtacctgaaa gagctttcag gcataggtgt gctgcaagaa atcgccatcg
     1501 gcagggacaa actattcatt catccgcgcc taatggaact attgcgggga gagggcaaca
     1561 gctttacttc attctaaccc cctcttcatc cctacatgac taacacgaaa cagggatttt
     1621 gacacccgaa ccggtacccc ttgtattttc cccgcgaaaa gccggcatcc gcccgcgtat
     1681 catgggagca acaaaacccc tgcctaaaat tttgacttgt gcaaattagg ggtatatttg
     1741 gggggtatat tgaaaaatgg ctaaaataaa atgtttaata aacaaaatgt tgaaacttaa
     1801 tttcgataga gcctctgcat atcgtattga ggcgttaatg gaatttgaga aagctatttt
     1861 taaataagaa aaggtaacta ttaaatagct acctttctaa aattaaatat caacacatcg
     1921 ttaaaacaca gccaaaaaaa accgaaaccg aacggtccgg attcccgcct gcgcgggaat
     1981 gacggatttc aagattacgg tattgtcggg gatgatggga aacggtggga attgtgtaaa
     2041 aaatgccgtc tgaagccttt caggcggcat ttgcggcatt cggacattta aaaatcccgt
     2101 cattcccgcg caggagggaa tccgaataca tccgcacaga aacctgcatc ccgtcattcc
     2161 cgcgaaagcg ggaatctaga acgtaaaatc taaagaaacc gttttatccg acaagtttcc
     2221 gcaccgacag gtctagattc ccactttcgc gggaatgacg ggattttagg tctctgtaat
     2281 ctcgggcagc tttccgttat cgttttaacc caaagacacc atttcaatct gctccatcgt
     2341 cctgcccaga aaacgctcgc cgatggttct gaatttcaaa ggaatatcgc tgacgtaaaa
     2401 acggtagtcg ggattgttgt tgccggtatc gagcaatcct tcctgtgcaa ggacgcgtgc
     2461 ggtttcttcg gccgtcgtaa tcgcggaatc gaccagcgcg acattgtgcg cctccctgcc
     2521 gattaagggc ttgagcaagg gaaagtgcgt gcagcccaac accagcgtat cgatgccgtc
     2581 tgcaagcaat ggtttgaggt attcgcatac ggtcaggcgg gtaacttcgt gttccaacca
     2641 accttcctcc accaaaggga cgagcagcgg cgcggcctgc gtgcggacga gcgtgtcggg
     2701 gttgtccctg tggatggcgc gcgcataagc attgctgtta actgtcgtat tggtggcgat
     2761 aatgccgatt ttgttgttgc gcgtcgttgc cagcgcggct ttggcgccgg cggaaatcac
     2821 gtccaaaacg ggcatattgc ccgtcttttg acggattttc cgccccgcaa ccgccgcaat
     2881 cgtgttgcac gcgataacca tcgccttgac atcgtgtccc aataaaaaat cgacaatctg
     2941 catcgagaaa ttttcgatgg tcgccttgga tttcgtcccg taaggcacgc gcgccgtgtc
     3001 gccgaaataa atgatgttct ccatcggcag ccgttccatc agcgcgcgca cattggtcaa
     3061 accgccgatt cccgagtcaa aaacgccgat gggccgctgc ctgccgatat tttccatcct
     3121 ttttccaatc cgtcctaaac atacaatccg catattgtac acgcgccgct ttttcttgac
     3181 agccatgccg tctgaaagca ggatgcggat ttggctgtta taatgtacgg caatgccgcc
     3241 gccgggcgca cggttttccg accgcctgcc cctccgatcc gccacataaa agttgtaaag
     3301 cccctatgtc tgatttttcc cccgtttccc gattccttgc cgacgaagcc gccacactcg
     3361 atttgggcgc ggcgtggtct tcccgtttaa acgcaccgct ggtcatttac ctcgaagggg
     3421 atttgggtgc gggcaaaacc acgctgacgc gcggcatcct gcgcggattg ggtcaccagg
     3481 gcgcagtcaa aagcccgacc tacgccatcg tcgaatctta tccgctggaa cgcttcgccc
     3541 tgcaccattt cgacctctac cgcttctcgt tccccgaaga atgggaagac gcggggcttg
     3601 acgagctgtt tgccgcaaac agcgtctgcc tgatcgaatg gccgcaacag ggaggcgaat
     3661 ttaccccgcc tgccgacatc accgcaacat tgacacacgg cggcggcggc agaaaatgcc
     3721 tgctgaccgc ccataccgaa cgaggacgcg aaagcctgcc gctatgacca aactgacacg
     3781 aagacaaatc atccgccgca ccgccggcac actgttcgcc ctaagcccca tcgcatccgc
     3841 cgttgccaaa acggtacgcg ccccgcagtt taccgccgca cggatatggc cgtcgcacac
     3901 ctacacccgc ctgacgctgg aaagcaccgc cgcgctcaaa taccagcact tcgcgctcga
     3961 caacccgggc aggttggtcg tcgatataca aaacgcaaac atcaataccg tattgcacgg
     4021 gctttcccaa aaagttatgg cggacgaccc ctttatccgc agcatacgcg cgggtcagaa
     4081 cacgccgacc accgtccgcc tcgtcatcga cctgaaacag cccacccacg cacaagtctt
     4141 cgcgcttccg cccgtcggcg gctttaagga ccgcctcgtc gtcgacctct atccgcacgg
     4201 gatggatgcc gacgatccga tgatggcgct gctcaacggc agcctgaaca aaaccctgcg
     4261 cggctctccg gaagccgacc tcgcccaaaa caccacgccc cgacccgggc ggggcaaaaa
     4321 cgggcgcaga cccgtcatca tgctcgatcc gggacacggc ggtgaagacc ccggcgccgt
     4381 cagcccgggc ggtttgcagg aaaaacacgt cgtcctctcc atcgcccgcg aaaccaaaaa
     4441 acaattggaa gccttgggtt acaacgtctt tatgacgcgc aacgaagacg tgttcatccc
     4501 attgggcgtg cgtgtcgcca aagggcgcgc acggcgggcg gacgtattcg tctccatcca
     4561 cgccgacgct ttcaccagcc cctccgcgcg cggaacgggg gtttatatgc tgaacaccaa
     4621 aggcgcgacc agctctgccg ccaaattctt ggaacaaacc caaaataatg ccgacgcggt
     4681 cggcggcgta ccgaccagcg gcaaccgcaa cgtcgatacc gccctgctcg acatgacgca
     4741 aaccgccaca ctgcgcgaca gccgcaaact cggcaaactg gtgcttgaag aattgggcag
     4801 gctcaaccat ctgcacaaag gcagggtgga cgaagccaat ttcgccgtat tgcgcgcacc
     4861 cgatatgccg tctatcctgg tcgaaaccgc cttcctgtcc aatcctgccg aagagaagct
     4921 gctgggcagc gaatccttcc gtcggcagtg cgcccaatcc attgcctcgg gtgtccaacg
     4981 ctacatcaat acatccgtat tgaagcgggg ttgattgaaa aggcggtgtt tcaaaatata
     5041 gttgtcggat gaaggcaggc ataaacctac ttccgcccta aaccgcgttt ttatgatgaa
     5101 aggtatggat aaattgagat accaacggga ttttttaaat atccgcccca tttttacagc
     5161 cggcgaacaa gaatacctga ccgaattgtc cgatcggctt cctttatctg ttttaacgga
     5221 ctccgtgcgt aatattgaag aaatcggtat cgattttgtg tacagtcctg ccaaattgga
     5281 aggaaacact tataatcaat acgatacgca ggcattgctt aaactcgggc aaaccgcagg
     5341 cgggaagtta tattcggatg ccgtaatgtt aatcaacctg agggaaagtt accggcacct
     5401 gctgtccggt ttggacagcc ccaagccgtt tgactggttg gatttcttaa aaaccaccca
     5461 cagcctgatt tcggaaaact tactggaaaa aggctccggc ggcgtagtcc gccgcgacag
     5521 cgttactata agcgggacag actacacccc tttatccaac ccgcaaagtt tggatacgga
     5581 attgaaatgg ttgcttcaag aagcccccaa aatagaaaat cctttcgacc gcgccgttta
     5641 tctccataat aatttggcgt atctccgata ttttaaggac tgcaataaac gtactgccag
     5701 aaactgtatg accttgtcgc tgatgcgctc cggttttttt ccttgcgtat tctcccccga
     5761 cagttatccc gcctatgccg aggctgtcgt agcctattac gaaaccggcg attacggttt
     5821 attcaagaaa tattttattt cggcatacga aaataccgta aataagtacg gtccccagcc
     5881 tgatgtggat attttccgaa atttctccat ttaaaaagcc gtctgaacat ttttcagacg
     5941 gcatttttgt acactttcct caacggttcg atccctgcac catatcgatc aggaacaggc
     6001 ggcagtcgag gttgccgtta taaagcggga ttttccgctt gggcagcagg cgcatgaatt
     6061 tgggcatttc cctgtcgccg gtaaacatgc cgacccgcca gccggcatag tgttttttca
     6121 accacgtccc caactgcgga taaagtgcct gcaaagcgcg gacttcctca aggcgcacgc
     6181 cgtagggcgg attggacacc atgatgccgt tttcgccgtt cggacggacg gactgcgcgc
     6241 cggcaacgct gaaggaaacg atgtcgtccg ctccggcatt gtgtgcgttg tccaatgccg
     6301 tctgaacgat gcggcggccg ttgtcgctgc ctgcaatcgg ggcgcggacg gggcgggttt
     6361 gcgcttcggc gcggcggcgc aaatccgccc acagcgtttt atcgaaattt tgcagttttt
     6421 caaaaccgaa gcggcgcatc atacccggcg cgcggccggc ggcaatccaa gcggcttcga
     6481 tagcaatcgt gccgctgccg caaaacgggt cttgaaacgg ctgcgtgccg tcgtagcctg
     6541 ccgagagcag cagcccggcg gcaaggtttt cgcgcagcgg ggcttcgccg gtatccaggc
     6601 ggtagccgcg tttgaacagg gcttcgcccg aagtgtcgat gaagatttcg acattgcgtt
     6661 cgtccaaaaa ggcgtggatg cggacatcgg gcgcggcttt gtccacgctc ggacgtgcgt
     6721 cgtaaatgtc gcggaaagcg tcgcagacgg catctttgac ggtcaggccg acaaattgga
     6781 ggcttttgac gttggcgcgt tttgcctcga ttttgacttt gaacgtctgc tgcaaggtaa
     6841 accagttgaa ccagttgatg tttttggcaa gtttgtagat gtcgcgctcg gttcggtatg
     6901 tgcctttggt caggcgcagc aggatgcggc cggcggtacg cgagtgcagg ttggcggcgt
     6961 aggcctgttc caatccgccc cggcaggaaa cgccgccgtc aaacgcttgg acatcggtac
     7021 agccgaggct tccgagttct tgagataaaa cggtttccaa gccgcgcggg caggtggcga
     7081 aaagtgtata taaagtattc atagcgtgtc ctttccggac ggatgttccg gcaatggcgg
     7141 attgaaaggt ttattcggcg gtaaatgccg tctgaaatgt ccggctgccg attgtaatgt
     7201 ccgccgtaaa atcgcgcctg ccttcgacac agacgggcag gcggatgcgt gctgcctgcc
     7261 aagtccccga cggttgccgc tcgaacatat agcggttgaa acccatatcc atatttttca
     7321 tactgaagct gatgctgacc tgttccgtgc cggcgggcgc gtgttcgata taaatatcaa
     7381 acggtttttt ggttgaaacg gcggcggcgc ggacacggct tccgtccggc agcgtgcaac
     7441 cctcggtcaa atcgcattgc gccgccacgg cttgcggctg ctgcgcctgc caccattgca
     7501 acaaaacgag cttgaaggcg gcaaaggcag tcagcagcag tgcggcaagc agtaatttgc
     7561 ggtttttatt catgattctt ccctattgtt tcatccctta ccaccgcgtg cgcccccaat
     7621 ccgagccata gttttccata acgtgcaacc gtctgtccgt ttgctgcaag tacgagcggc
     7681 acggatacgc cctgccgcag ggcttctgcc gcctcttgtg cctgaacatc atcgcacacg
     7741 cgccacacga ccacatccgc gcccttttcc tgcgcctgcc gccattgttc gcgggtctgc
     7801 accatcatcc acacgctgcc ggcgtgttcg gacctgtcct gccattgtgc cgcctccatc
     7861 aaaacgttcg caccgcttcc ccctgccgaa cccaaaggca ggacgcggta cgcgcccata
     7921 ctgttttcgc cgtacaaacc tgttttcagg ctgccgtaca aacggcgcgg gacggacagc
     7981 gaacgcaaaa gcgcaccgtt ggcgggcagc atgggggcaa cggtaaaatc acccgccttc
     8041 tgccaagacc attcctgccc ttcgcgcgat tgcggtccgc cctcccattg atcggggttt
     8101 acccataaga atttcaggca gacgcgggcg tgttcgtagg aatggatttt ggtcagccaa
     8161 ggcgtggcgg cgaggatgcg gatgccgagt tcttctccaa actcgcgttg cagggcttgg
     8221 aagtcggttt cgcccgcctc gaccttgccg ccggcaaatt cccaatatcc ggcatagggc
     8281 ttgccttcgg ggcgcgagcc gagcaggtaa ttgccgtctg aatcgagcag gatgccggca
     8341 acgacgcgga taagggggcg ggtatcttgg atcatggcgg gtatgcggtt ggtcggtcgg
     8401 aatgcaaatg agggaagggc aatgccgtca acgggtttgc cgaatttcag acggcattat
     8461 ttttcggcaa cggcaaacgc caacacggta tcgccttcgt ccgccatact caggctgacg
     8521 cggctgatgc cttgttcttc cagccattcg gacagggcgg ggccgtagaa aaattcgggc
     8581 ttgcccaatg cgtcgtgccc gatgccgatg ttgcggaagg aaaccgcgcc tcgtatgccc
     8641 gtgccgacgg ctttggcgaa ggcttctttg gcggcaaagc gtttggcaag gtagttgacg
     8701 ggtttgcccg cctgcggaaa ttcaagcagc tcttcgggag tgagtatgcg ccctgcaaac
     8761 gcctgtccga attttttgtt taagcggatg atgcgcttga gggaaacgat gtctgtaccg
     8821 atgccgtaaa tcatatttgc gctccttttt cgtgaaatgc cgtctgaaac ggtttgcctg
     8881 cccttacggc agcagccttg ccctgaacat cgcctccttc atctggcgca cggcttcggg
     8941 aagtccgagg aagagggctt gggcaatcag cgaatgcccg atgttcagtt cgcggatggc
     9001 gaggatttgg gcgacggggg taacgttgtg tatggtcagt ccgtgtccgg cgttgacgac
     9061 caggcccaaa tcgccggcga aatgcgcgcc gttttggatg cgctcgaact gcctgatttg
     9121 ttcggcgtgg ctttgtgcgt cggcatatgc gccggtgtgc agctcgacaa cgggcgcgcc
     9181 gacatcatag gcggcttgga tttgcctgtc gtcggcatcg ataaacaagg acacgcgtat
     9241 gcccgcgtcg gtcaggattt tggcgaattc ggcgattttt tcctgttgcg ccaatacgtc
     9301 caaaccgcct tcggtcgtga tttcctgccg tttttcaggc acgatgcaca cgtcttccgg
     9361 catcacttta agcgcgtttt cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg
     9421 cgtgcggatg gcgtttttga cggcaaatac atccgcgtct ttgatgtggc ggcggtcttc
     9481 gcgcaggtgc atggtaatca ggtctgcacc gtgcgtttcg gcaaccagtg ccgcctccac
     9541 ggggctggga taagtcgtgc cgcgcgcgtt gcggacggtg gcgatgtggt cgatgttgac
     9601 acccagaagc ataatgtttc cctttctctt cgttttcctg tttgacggca tggtgcgggc
     9661 gcagctacgc ggtattgcgg tcgaactgcc ttatctgttc caacacctgc cgcgatttca
     9721 agccttcggg aagcagcgtg ccgattagca gccgtgtgat tttcaatgcc tgttgcaggc
     9781 tttcggcggt gcggaaactg ccttcgcgca aatcgatcag gctctgccct tcgacaattg
     9841 cgccggcttc gtgcgaaagc gcgcctgtgt cccggcatac gggcatgacc gcctcttcgg
     9901 gcatcagccg gtaggttttg tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat
     9961 cgggggcaac gcccaaggca ttgagcagct tccattcgaa gcggcgcaaa tcggcaatat
    10021 ggttggcttc acggcacact gcctccatca cttttgccaa cgcgtcgtac agttcggaca
    10081 tcgggtcttc gcgcgcggtc agtttcaaca ccagctcgtt cacatacaga ccgctgaaca
    10141 acgccctgcc ctgcggctgc cgccatccgc ccatccattc ggcgcggtgc agggttttga
    10201 gttcctgact gccgtaccac gacacgctgg cagggacgaa cggaaccaat acgccgcgca
    10261 gctcgctctg ccgtttgcgc gcgctgcgcg ccagcaaagc cacacgcccg taacggcggc
    10321 tgaatgcttc aacccgcagg ctgctttcgc gccaaggcga agatgccagc ataaaaacgg
    10381 gttcgtggtt gactcggtgt tcggacataa tcgttgtgga aatgccgtct gaacgctgcc
    10441 gcgactacaa aacggcagtc ggatacgaac cgatggcttt gacaaacgaa gcgcgttcgc
    10501 ccaagcgttc caatgccgtc tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga
    10561 agaacaggta ttcccacaaa acggatttgc tcggacggct ctcaaacttg gtcatggaaa
    10621 tacccgattc ggtcagcggt tgcagcagcg aggcaaccgc gccggcgcgg ttgggcgcgg
    10681 aaacagccag cgaggttttg tcgctgccgc ttgcaccggt ttcgtgatgt cccatgacca
    10741 gaaagcgcgt ggtgttgttc ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt
    10801 aaatttccgc cgccgtgcgt ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa
    10861 ccagccttgc ggcttcggca ttgctggaca cggcaatccg ttcggcgttg ggcagatgcc
    10921 tgcccaacca gtcgttgcac tgcgccaacg cctgcgcgtg cgaaaatact ttggcgatac
    10981 cctcggtgct gccgttgttt ttgcgcaaca ggttgtggcg gatgcgcaaa acgacttcgc
    11041 cgcacgcctg caacgcggta acggcaagca ggtccaacgt gcggccgacc gagccttcgg
    11101 tcgagttttc cacaggggcg accaaataat ccgcctgacg ggtttcaacc tgtttgaagc
    11161 agtcgtctat ggtcggacac gccattgtat gcgcggcgtg tccgaaatgc ttgattgccg
    11221 cctgttgggt aaacgtgccc tgcggcccca gataggcgat ggtcagcgga cgttcgacgg
    11281 cgaggcattc gctcatcact tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc
    11341 ctttgttcaa atcctgaatg cggcgcaaca cggcaacctc gcgttcgggg cggtacaccg
    11401 cgcccgtgcc tttcagctcg ccgatggcgt gggcatgttg cgcgcgttcg ttgagcaggc
    11461 gcagaatttc ggcatcgatg gtgtcgatgg cgttgcggtg cgggatgagg agttcgtcga
    11521 tagtttggga cataatttcg cctgaacggt aatttgccgt gcattctaac atcaaatgcc
    11581 gtctgaagcg gtttcagacg gcattttccg ttaaaggtat tcgctttgcc cgttttcttt
    11641 ccacgcacga tgcgagcaga gccacagaag cgcaatgccg caagaggtag ggccgccata
    11701 gttgccgcca tcacggttgc cgaaccgtcg tgcaggacgg ttgccgccat gcccaccccc
    11761 gcgccgatta aggaccggaa tacacccgac acggcgttcg cgctgccgcc ctcttctttg
    11821 aaataagaca taaagcatgc ctgcgtgtcc gcaccgacca ggccctgcgt accgacggaa
    11881 aacatcacgc acgcgaccgg cagccaaaac gggggcaacc cgaaaaacag cacggcggcg
    11941 agttgggacg ggttggcggc aaattggacg acaatccccc gcagcaggat gctttgcgga
    12001 tgcgcgccgg ttttaagccg ccacgcggta acgcggctga aaaacatcat cgtgatgatg
    12061 ttgagtgcaa acacccatgc gtaccggtgc ggcgtaacgt ggtagagctg ccggtacacg
    12121 aaggaagatt cggtcagaaa ggcgaacatc gaaccgaagc tgaatgcctg aaaaaacaga
    12181 taacccatcg cggcacgggt tttcaatacg cgcttgaacc gccccgccac cagcccgaac
    12241 acatccctgc cgattttgcc gccgacggcg ggattgggca ggaaatactg taccaaaccg
    12301 ggcagcaccg gcgaatacgc cgccaagaaa acgaaaatcg cccgccatcc gcccaatccc
    12361 tgcaacaatg cgccgaccat gggtccggcc agcggcacaa ccatcagaat gatgccgata
    12421 agggcaaaca tctgcgcggc tttgcgtccg gaataataat cgcgcaccat cgcaccgacg
    12481 atgactacag ccatgcctgc gccgaacgcc tgtaccgcac gcaggttaag gagctgttcg
    12541 gtactcgaag caaatacgat ggcggcaacg gcaaggcaat atacaatcaa accggtcagg
    12601 gcgacgggtt tgcgcccttt gatgtcggac accgcgccgc cggccacttg cccgaacgcc
    12661 gtgccgaaca taaacaaact cagactctat tcgatacggt ggatatccgc gttcagcggc
    12721 tgcgccattt cgggaatcgc gggcaggtag gcatcggtgg aaaacggcat cagcgcgacc
    12781 agtaccgcca tcaaaaccgc catcagtttt ccgctcatcg gaggaagggt cggagacatg
    12841 gtttttccgt tcaaatcaaa taaagaaacc gtcgcaaacg acggtcggta cgggcgcaag
    12901 tttaacatat cggacggcgg ggcaaaaccg aaggatatgc cgcccgaaac cgcttcagac
    12961 ggcattgccg ttttgcgcgt ccctgcgcga cagccggccg gcggcgaaag tggcggcgat
    13021 gacgacaaac acgcccaagg cggacacggg cgataaatgt tcgcccaaaa tcaaaaccgc
    13081 caacagcacg ccgacgacgg gttcgagcga aatcaacagt cccgacgcgt tggcaggaac
    13141 acggctcatc cccttgttcc acagccaata ggcgtaccag ccgcacccca aacccaaata
    13201 caacagcgac aataccatcc cgacgctcca gtccacggta taactttgcg ccaaagcaag
    13261 cgaaaacggc aggcacatca acgatgcggc ggcaatggaa acagatgtga atgccggtgc
    13321 gccgatgcgg gcaatcagcc tttgcgtcgg acgcatagcg gcacaaaagc ccgcgcccgc
    13381 caacaacacc agcaggcagc cgaaccagcc gacttcgccg ccctcttccg caccgcccgc
    13441 catcagcagc gcgacaccgg caaatgccgc cgcgccgcat atccagtggt aggcacgcgc
    13501 tttgtcgttg aagaaaaagt gtccgacaaa caccatcagc agcggctcga gtccgacaat
    13561 gaccgatgcg ctggcggcgg aagtgtattt caacccgaca aactgaagca gcagggtcag
    13621 cacatagttg acgaacgaca caatcagcaa cggcttccat tcctcacgcg gaatcttgcc
    13681 gacatgacgg cggcaggcgg gcagtgcagg cagcgcggca atcagcaggc gcacgccgac
    13741 catcaatgcg ggatcgatgc cgccatagac atatttggcg gcaataaacg agctgcccca
    13801 gataatcagg gcaaggattt ggtaaaacat agtgagtggt aaagtcagat ggtcatacaa
    13861 aagtatagcg gattaacaaa aatcaggaca agaaggaatg cccgaaccgt cattcccgcc
    13921 atttttcgtc attcccgcga aagcgggaat ctaggacgca gggttaagaa aacctacatc
    13981 ccgtcattcc ctcaaaaaca gaaaaccaaa atcagaaacc taaaatcccg tcattcccgc
    14041 gaaagcggga atctagaatc tcggactttc agataatctt tgaatattgc cgttgttcca
    14101 aggtccggat t
//
LOCUS       DAXFHF010000057        13387 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXFHF010000057 DAXFHF010000000
VERSION     DAXFHF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.057"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            1..1614
                     /locus_tag="ACFIRM_001616"
     CDS             1..1614
                     /locus_tag="ACFIRM_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3029208.1"
                     /translation="MRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCVW
                     GAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRPQ
                     LLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARYR
                     ALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTEG
                     LTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTAL
                     ADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSGS
                     DGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRAE
                     RNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGWA
                     QEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPTL
                     NYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1675..3549)
                     /locus_tag="ACFIRM_001617"
     CDS             complement(1675..3549)
                     /locus_tag="ACFIRM_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC3029209.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGIISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3701..4631
                     /gene="ppk2"
                     /locus_tag="ACFIRM_001618"
                     /pseudo
     CDS             3701..4631
                     /gene="ppk2"
                     /locus_tag="ACFIRM_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            complement(4713..6144)
                     /gene="aldA"
                     /locus_tag="ACFIRM_001619"
                     /pseudo
     CDS             complement(4713..6144)
                     /gene="aldA"
                     /locus_tag="ACFIRM_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(6252..7167)
                     /locus_tag="ACFIRM_001620"
                     /pseudo
     CDS             complement(6252..7167)
                     /locus_tag="ACFIRM_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7340..8140
                     /locus_tag="ACFIRM_001621"
     CDS             7340..8140
                     /locus_tag="ACFIRM_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3029210.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8188..8964
                     /gene="mlaE"
                     /locus_tag="ACFIRM_001622"
     CDS             8188..8964
                     /gene="mlaE"
                     /locus_tag="ACFIRM_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC3029211.1"
                     /translation="MNFIRSVGAKTLGLIQSFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9015..9509
                     /gene="mlaD"
                     /locus_tag="ACFIRM_001623"
     CDS             9015..9509
                     /gene="mlaD"
                     /locus_tag="ACFIRM_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC3029212.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9540..10130
                     /locus_tag="ACFIRM_001624"
     CDS             9540..10130
                     /locus_tag="ACFIRM_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC3029213.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGTSLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10181..10459
                     /locus_tag="ACFIRM_001625"
     CDS             10181..10459
                     /locus_tag="ACFIRM_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC3029214.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10456..11283
                     /locus_tag="ACFIRM_001626"
     CDS             10456..11283
                     /locus_tag="ACFIRM_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC3029215.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11408..11770
                     /locus_tag="ACFIRM_001627"
     CDS             11408..11770
                     /locus_tag="ACFIRM_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC3029216.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11770..12153
                     /locus_tag="ACFIRM_001628"
     CDS             11770..12153
                     /locus_tag="ACFIRM_001628"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3029217.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKPPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            12150..12638
                     /locus_tag="ACFIRM_001629"
     CDS             12150..12638
                     /locus_tag="ACFIRM_001629"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC3029218.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12872..13000)
                     /locus_tag="ACFIRM_001630"
     CDS             complement(12872..13000)
                     /locus_tag="ACFIRM_001630"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC3029219.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(13006..13368)
                     /locus_tag="ACFIRM_001631"
     CDS             complement(13006..13368)
                     /locus_tag="ACFIRM_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC3029220.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
BASE COUNT         2835 a         3764 c         3845 g         2943 t
ORIGIN      
        1 atgcgccgcg tccgccgcgc gtggggcttg cggcgtttct gccgccgccg tccggcggtg
       61 ttttttaagg aaggcaaatt gaaggatagt gtgtatcggg tatgccggct gtgtgccgcg
      121 ctgtgtgttt ggggtgcggt cggcgcgtat gccgccggct tgcccgacgt tcgggacgat
      181 gcggcggcgt tgcgcgccca gcgtgcggct gcggagggtt gggcgggaat gccgccggaa
      241 ggggattctg ccgcaaacgg cggttcgcgc gtaatcgacg gggatttcct gctgtcgcgc
      301 ccgcagttgt tggaacacgt tttgcgcgac gcgctcaacg gcaatcaggc ggatttgatc
      361 gcttcgttgg cggatttgta tgccaagctg ccggattatg atgcggtgtt gtacggcagg
      421 gcgcgggctt tgctggcgaa attggcggga aggccggcgg aggcggtggc gcgctatcgg
      481 gcgttgcaca gggataatgc ggcagacgag cgggttttgc tggatttggc ggcggcggag
      541 tttgacgatt tccggctgaa gtcggcaggg ctgcattttg cggaggcggc aaaattggat
      601 ttgccggcgc cggttttgga aaatgtgggg cgtttccgga aaaaaacgga ggggctgacg
      661 ggctggcatt tttcgggcgg catcagccct tctgtgaaca aaaacgccaa caatgccgcg
      721 ccgcaatact gcctgcaggg cggcggcagc cggatatgca gcgttaccgg acctgtccgt
      781 gcggcagggt tggattatga ggccggggcg gaaaagctga ctgcgctggc ggacaaccat
      841 tatctgctgt tccgcgccaa tgtcgggggc acaagctatt atttcagccg caaatcggct
      901 tatgacgatg ctttcggcag ggcttatttg ggctggcagt ataaaaatgc gcggcagacg
      961 gtgggggttt tgccgtttta tcaggcgcaa ttgtcgggta gcgacggctt tgatgcgaaa
     1021 acgaaaccgc ctgccgaccg ccggcttgcg ccgtatatgc tggcgcacgg ggcgggtgtg
     1081 cagttggcgc attcttaccg cctgagccgc cggtcgcagc tttcggtgtc gctggaacgt
     1141 taccgccaac gctaccgcga acaaggccgg gcggaacgca acgacggttg gcaggacggg
     1201 ctttatgtgt cgctggcaag gcggttgggc ggctcggcaa ccgtgttcgg cggctggcag
     1261 tttgcgcggt ttgtgccgaa acgcgaaacg gtgggcggcg cggtcaacaa tgccgcctac
     1321 cgccgcaacg ccgtccatat cggttgggcg caggagtggg gcgggacggg cggtttgaac
     1381 agccggattg ccgcgtctta cgcccaccgc aactacaaag gcattgcggc attttcgacg
     1441 gaggcgcagc gtaaccgcga atggaatgtc tcgctggctt tgagccacga caagttgtcg
     1501 tacaaaggca tcgtgccgac gttgaactac cgcttcggta agacggaaag caatgtgccg
     1561 tatgcaaaac gccgcaacag cgaggtgttt gtgtcggcgg attggcggtt ttgaatggtg
     1621 ggataatgcc gtccgaactt tgcggcagca ggttcggacg gcatttttgc gcgttcaggc
     1681 aaggacggcg gcaaatacgc cgcgcaaggc gttggagagg cggatttctt cggctccttg
     1741 cagtgttttt tgtgtgatgt gtgtttcaat cacttgattt gtgtgcaaat atttttgcgg
     1801 ttcgtccgac acggcttggc gcattatgcc gtttaaaatg tctaaatcta aagagggtgt
     1861 gagccattgt ccgcgatgtt tgacgaacac gttgctcctg ccgccttcga gtaggatgcc
     1921 gtccgaattg aaaaacaggc tgtcgaacgc gccttgcgtt tcggcggttt gccacgcttg
     1981 gtcgaagatg gcgcggtgtg tggttttgaa gcggcgcagg tagttttgtg cgggcaggat
     2041 ggtcggcgaa atgatgacgc gctgtttgtc ggtcagacgg tttaaaacgg cgtgggacag
     2101 gctgatgccg tctgaagcga gcagggcttt gatgcggaac gcgccgtcgg gcaagtcggc
     2161 aatgtattgt ttgatttgat tttcgcagcc gtcgggcagg ggcaggttga gggcttgggc
     2221 ggaggttttc agacggcata ggtggcggtc gagcagggcg cattgcctgt tttccgcgcg
     2281 cagggtttca aaaatgccga aatcggggcg cagttcgttg aggaatcggg ctttccagcc
     2341 gcattcgcga tattcggcgg cggggtcgct gtcgatgacg atgccggaac cgacaccgta
     2401 cacgccccga tacggcggat tggcttcaaa cggatgcggc gtttccccgc cttgtcctgc
     2461 cgttcgggct tgcgcgtttg aatccgtgcc gccgatgcct gaaacaatgc cgtctgaaac
     2521 gggtttgagc gacagggtgc ggatgacgac gttgaacgcg ccttcaaacc ccaagccgcc
     2581 ggaacacggg ttcaaatagc cgatgctgcc cgtataaagc ccgcgcgctt cggtttcgag
     2641 cgattcgata atctgcatac tcattttttt gggcgcgccg gtgatgctgc cgcaggggaa
     2701 ggcggcgcgg agggtgtcgg cgaacgaggt gtccggcagg gcttgcgccc ggatggtgct
     2761 ggtcatctgc cagacgctgc cgaaacgcga tactttaaac ggctcgggta cgcatacttt
     2821 accggtttgg gcgattttgc cgaggtcgtt gcgcagcaaa tcgacaatca tcacgttttc
     2881 ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg cgttcgtctt gtccgtcgcc
     2941 caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg atgccgtctg aagcgatgtt
     3001 gaggaagagt tcgggcgaga aacacagcgt ccacgcggat tgcccggctt catcgggcag
     3061 gtgggacaat acggcatagg ggacgggctg gcgcaggcgg cggtagaggc tgacggggtt
     3121 gccgtaggct tgcaggtgca ggcgggtggt gtagttgatt tgataggtgt cgccgcgccg
     3181 gatggcttcg tggatttggc ggatgcggtc gaggtaatcg gtttcggata cggaggattg
     3241 cggcgtggaa ataccggcgg ggatgccgtc tgaatgtcgg gcaagccagc tttcggcatc
     3301 ggtgtcggcg cagtcggcaa accagtgcag ggcaatgttg ccgccgcgtt cggattccat
     3361 ccccatcagc ggcaaaccga attcgtagtc tgcaaacaac accgaatgca gccctttttg
     3421 ccatcccgat tgcagcgcgc cgtctaaagc atcgagttct tcgggacgga aaaaacggct
     3481 ttccacatga ttttgataga gttttgcgcg tcggctgacg gcatcgtcaa acagggcgaa
     3541 ataaggcatg gcaattcggg gcaaatgttt tgattatacg ccccttacgg cggggcaaat
     3601 tcgggcaaaa tgtaatttta tgtagagaaa gcggtggcga aagtgtaaaa ttgtgaaaac
     3661 ggactttcat ttccaacctg aaatacaata ggagaccttt atggcagacc gccagttgca
     3721 accgtttgaa aacgtagaat taggcgaaaa gcaagaccag ctccaggtat ttgaaaaagc
     3781 tgttttggaa cacgaaggca aaggttctgc cgaagattcc ggcacggccc cgctgcccga
     3841 aaactacccc tgccgcaaac gtatgctccg cgccgcctac gaagccgaaa aagccaaact
     3901 gcaaatcgaa ttgctgaaag tgcaaagctg ggtcaaagat tccggccagc gcatcgtcag
     3961 cctgtttgaa ggccgcgacg cggcaggcaa gggcggcacc atcaaacgct ttatggaaca
     4021 tttaaacccg cgcggcgcgc gcgtggtcgc tttggagaaa ccgactgaca ccgaacgcgg
     4081 acaatggtat ttccaacgct acatccaaaa cctgccgact gcgggcgaaa tggtattctt
     4141 cgaccgctcg tggtacaacc gcgccggcgt ggaacgcgtg acgggcttct gcgaacccaa
     4201 cgaagtatat gctctttatg cgccaagccc ctgagttgga acgtatgctt gtcgccagcg
     4261 gcatccacct gttcaaattc tggttctccg tatcccgcga agaacaactg cgccgcttca
     4321 tctcccgccg cgacgacgcc ctgaaacact ggaaactttc ccccgtggac atccagtcgc
     4381 tcgaccgctg ggacgactac accgaagcca aaaacgcgat gttcttccac acccacaccg
     4441 gcgacgcgcc ttgggtcatc atccgttcgg acgacaaaaa acgcgcccgt ttgaactgta
     4501 tccgctactt cctgcaccag ttggactacc cgggtaaaga cgtgaaagcc atcggcaaag
     4561 tggacgataa aatcgttctt gttcccgata cgcgttacaa agagaaaacc atcgatgtcg
     4621 gtcatgactg attgccggtg aagtaataaa aatgccgtct gaacccgatt tcaggttcag
     4681 acggcatttt ctatccgtgt gtcaaaccgg catcagatgt cggtttccag ataaatgact
     4741 tgggtttgca ggtattcttc caaaccgtgt ttgccgtccg cgccgccgat gccggatttt
     4801 tcccagcccg cgtggaagcc ctgcatggct tcgaagtttt cgcggttgat gtaggtttcg
     4861 ccgaattgca ggcggcgggt aacgtagaag gcttcgttca ggttggtggt gtacacggag
     4921 ctggtcagac cgaattcgca gtcgtttgcc agggcgatga cctggtcgag cgtgtcgaaa
     4981 gcggaaacgg gcagcacgag gccgaaagtt tcttctttca taatgtccat actgttgtcg
     5041 gtgtcggtca gcagggtagg ctcgaagaaa tagccgcgtc cttcggtgcg tttgccgccg
     5101 caaaccaatt tcgcaccttg tttgacggcc cgttccactt tttcggcaac ggctttgacg
     5161 gcgcgctctt caatcagcgt gcccatttcc ggcgcgcctg cttcggcttc ggcagggttg
     5221 ccgtagcgca cgcctttcat cgcggcggtc attttttcaa tgaacgcgtc tttcaggctg
     5281 ctgtggacat agacgcgctc ggcgcagttg cagatttgac cggtgttgcc gacgcgcgaa
     5341 gccaagatgg atttcaccgc taaatccaaa tccgcgtctt tcaacacgat ggcgggggct
     5401 ttgccgccga gttccagcga gactttggtg atgttggcgg aagcggcttc catcacttgg
     5461 cgacccgctt caaccgagcc ggtcaggctg accatatcga cttgcggatg ggcggacaag
     5521 gcgttgccga tttccgcacc cggaccgtcc accacgttga acatgcctgc gggcagtccg
     5581 accgcatcga tgatttcggc gaagatgtgg cagttgatcg gggttacgct gctgggtttg
     5641 acgacgatgg tgttgcccgt aaccagatcc gggcccattt tgcgggcgat caggaagaag
     5701 gggaagttcc gcggcaaaat gccggcaatc acgcccagcg gacgtttgaa cgataaaatg
     5761 ttttcgcgcg ggcggtcgct ttggatgatt tcgccttcgt aacggcgcgc ccattcggct
     5821 tggtaatcga gatagtcggc agtgaacatc acttccacac gcgccaagtc tttggttttg
     5881 ccgccttcgg caacgatggt gtcggtcagc tcgtcggcgc gtccgcgtat gccttgggcg
     5941 attttacgca aatacgcgcc gcgttcgacc gcaggcagac gctcccaagc cggttgcgcc
     6001 gcgcgcgccg cggtcaacgt ccgccctgcc gcctttgggt tcgcgggcga tggtttcttc
     6061 ggtggacggg ttcaatacgt cgcgccattc gccgttgaaa tcgttttcaa agcgtccgtt
     6121 gatgtacatg gccaattgtt tcatttcggg ttctccagtt ttgtagtcgg atgtagtttt
     6181 ggtttattcc caaataaatt gccgtacaag tttctttaca cgcggatttt gggtttcaag
     6241 tcaaatacgg cttaccggtt ttctttttta gtttccgcag gcgtgctgcc gaaatattgc
     6301 ctgaatgcct gaataaagct ggaaacgtgg cgctagccgc acaaatacgc ggtttcgccc
     6361 acgtttttgc ccccgttttg caacagatag agcgcgtgct gcatttgttt gtggtgcagc
     6421 cactcgcttg ccgtaatgcc gaaatggtcg cgcatacggc gttgcagcgt cctttcgctg
     6481 atgttcagcg cggctgtcag ccggttgact tggtgtgcgc cgccgtcgaa cgcggcattc
     6541 agggtgcggc tgaagtcttc agacggcata gcgtctgctt ccgccgtttg ccccgccgcc
     6601 ggctcgatgc cgtctgaaac cgtgtcccac aaatccgaca gcagccgcaa cacgtccgcc
     6661 tcgcggcgca atgtttcgcc caaatgcccc tttgggacgg tttgcaggca ggatgccgcc
     6721 aagccgcgca ggtttggggg caaatcccat atcctgaccg gttcgcggta aagcaggggt
     6781 gcgaaacgcg cgtattccgg acgcggcagc cattgttcca tacctttaat ggtcattttg
     6841 accgttttgc cgccgcgata aagatagcgg ctgaacagga cttcttcccc gacggcaatt
     6901 aggacaatct tgccgccgtc cgcatcgatg cggaagcggc ggcggttgat gccgaagtcc
     6961 aaacgccctt ccagcaacag cacgaacgac acataaggtt cggcgaggcg gctgctgcaa
     7021 aaatcgcacc gtgccgttac cgtaccgccg tggatggaaa tgccgttgga caaagtatcg
     7081 aaacggtagc tgccctccac ataagccgcc gccgcttttg accagattga cgaaccggct
     7141 gctgttcaaa tgttctgctt tttccatagt tttctgataa aggttatcat ttgaaagata
     7201 acatttttcg ccacagcaaa caatccgccg ccgacaaagg cattttcaca acacttgccg
     7261 tccgtatgcc gtctgaaaat tgcccgtctt cccgataagc cttaaaatac acgccgtcaa
     7321 acctaccgtc cgcccgtaca tgagcccatc cccctttatc gaaatgaaag acgtcgcctt
     7381 cgcgtatggc gaccgcccga ttctgaacga catcaatttc agcattccgc aaggcaattt
     7441 tgccgccgtg atgggcggtt cgggcagcgg caaaaccacg ctgatgaggc tgattacagg
     7501 acagattcgt ccgcagtccg ggcaggtttt gattgaagga cgggatttgg cgggtttttc
     7561 ggctgacgaa ctctacgaac accgccgccg tatgggcgta ttgttccaac acggcgcgct
     7621 gtttaccgat ttgtcggtat tcgacaatat cgcttttccg atgcgcgaac tgacgcaact
     7681 tccggaagcg gttattcgcg atttggtttt gttgaaattg aacgcggtcg gtctgcgcgg
     7741 cgtggaaaac ctgatgccgt ccgagttgtc cggcgggatg tcgcgccgcg tcgcgctggc
     7801 acgcacaatc gcgctcgacc ccgaaattat gttgtacgac gaaccgttta ccggcctcga
     7861 cccgatttcc ttgggcgtga ttgcccactt gatcagccgc gtcaacaagg ccttgcgttc
     7921 gaccagcatt atggtaacgc acgacattga aaaatctttg gaaatcgtcg atcaggtaat
     7981 tttcttggcg cacggcgaaa ttatgttctc cggctcgccg caggaaatgc gcgaactgga
     8041 ttcgccttgg gtgcgccagt ttgtcggcgg gctggcagac ggccccgtgg cataccgcta
     8101 tccggcgcaa acgtcgttgc agcaggattt gctcgggtaa atgagcaggt gccgtctgaa
     8161 accgttgaac cttacatata gaaatttatg aattttatcc gttccgtcgg ggcgaaaacc
     8221 ctcggcctta ttcaatcctt cggcagtatc acgctgtttc tgctgaacat tttggcgaaa
     8281 tccggcacgg ctttcgcccg tccgcgcctg agcgtgcgcc aagtgtattt tgccggcgtg
     8341 ctgtcggtgc tgattgttgc cgtttcgggg ctgttcgtcg gtatggtttt gggtttgcag
     8401 ggctatacgc agttgtcgaa attcaaatcc gccgatattt tgggctatat ggtcgcggct
     8461 tctctgttgc gcgaactggg tcccgtgttg gcggcgattc tgtttgccag cagcgcgggc
     8521 ggtgcgatga ccagcgaaat cggtttgatg aaaacgaccg gacagctcga agcgatgaac
     8581 gtgatggcgg tcaaccccgt cgcccgcgtg gttgccccgc gtttttgggc gggcgtgttt
     8641 tctatgccgc ttttggcttc gattttcaac gtcgcgggca ttttcggcgc gtatttggtc
     8701 ggcgtgagct ggctgggttt ggacagcggt attttctggc cgcagatgca gaacaacatt
     8761 acgatacatt acgatgtaat caacggtttg atcaaatccg ccgcgttcgg cgtggcggta
     8821 acgctgattg ccgtgcatca gggcttccac tgcatcccga cttcggaagg cattttgcgc
     8881 gccagcacgc gcacggtggt ttcgtccgcc ctgacgattt tggcggtcga ttttatattg
     8941 accgcgtgga tgtttacaga ttgacagact gtctgaagac gaaacgcgga acatcggata
     9001 ttcaaggaac tttaatgaaa aagaacatat tggaattttg ggtcggactg ttcgtcctga
     9061 tcggcgcggc ggcggttgcc tttctcgctt tccgcgtggc gggcggcgcg gcgttcggcg
     9121 gttcggacaa aacttacgcc gtttatgccg atttcggcga catcggcggt ttgaaggtca
     9181 atgcccccgt caaatccgca ggcgtattgg tggggcgcgt cggcgctatc gggcttgacc
     9241 cgaaatccta tcaggcgagg gtgcgccttg atttggacgg caagtatcag ttcagcagtg
     9301 acgtttccgc gcaaatcctg acttcgggac ttttgggcga acagtacatc gggctgcagc
     9361 agggcggcga tacggaaaac cttgctgccg gcgacaccat ctccgtaacc agttctgcaa
     9421 tggttctgga aaacctgatc ggtaaattca tgaccagttt cgccgagaaa aacgctgagg
     9481 gcggcaatgc ggaaaaagcc gcagaataaa catacacatc cattttcagg aaagacatca
     9541 tgaaaaaatc ctccttcatc agcgcattgg gcatcggtat tttgagcatc ggcatggcat
     9601 ttgcctcccc ggccgacgca gtgggacaaa tccgccaaaa cgccacacag gttttgacca
     9661 tcctcaaaag cggcgacgcg gcttctgcac gcccaaaagc cgaagcctat gcggttccct
     9721 atttcgattt ccaacgtatg accgcattgg cggtcggcaa cccttggcgt accgcgtccg
     9781 acgcgcaaaa acaagcgttg gccaaagaat ttcaaaccct gctgatccgc acctattccg
     9841 gcacgatgct gaaattcaaa aacgcgaccg tcaacgtcaa agacaatccc atcgtcaata
     9901 agggcggcaa ggaaatcgtc gtccgtgccg aagtcggcat ccccggtcag aagcccgtca
     9961 atatggactt caccacctac caaagcggcg gcaaataccg tacctacaac gtcgccatcg
    10021 aaggcacgag cctggttacc gtgtaccgca accaattcgg cgaaatcatc aaagccaaag
    10081 gcatcgacgg gctgattgcc gagttgaaag ccaaaaacgg cggcaaataa ccgacaaacc
    10141 ataccgtccg agggcggtat gggtttttcg aacaaacgct atgcatacag aactcaaaaa
    10201 cggaacactg cacatcggcg gcgacatcac cgtcaaaacc ctgaccgggg acgcatttgg
    10261 gcgtttccgg caacaatgcc gtctgaaaga aaccattgcc gtcgatttcg gaggtgtcaa
    10321 acgtgccgat tccgcctgcg tgtcgctgct gctcgaagtg ctgcgcggct gcaaaggcag
    10381 cgtcaggctg accggcattc ccgaatccgt gcgcgcgctg tccgaactgt acgaaatcaa
    10441 agactggctg aaatcatgaa aaaaaccgcc tacgccatcc tcctgctgat cgggttcgct
    10501 tccgcccctg catttgcaga aacccgcccc gccgaccctt atgaaggcta caaccgcgcc
    10561 gtttccaaat tcaacgacca agccgaccgc tacattttcg cccctgccgc gcgcggctac
    10621 cgcaaagtta cgccgaaacc cgtccgcgcc ggcgtgtcca atttttttaa caacctgcgc
    10681 gacgtggtca gtttcggcag caatatcttg cgtttggaca tcaaacgcgc aagcgaagac
    10741 ctcgtccgcg tcggcatcaa taccaccttc ggtttgggcg ggctcattga tattgccggc
    10801 gcgggcggcg ttcccgacaa taaaaacact ttgggcgaca cgtttgcctc gtggggctgg
    10861 aaaaacagca attatttcgt gttgcccgtc ttaggcccgt ccaccgtccg cgacgcgctc
    10921 ggcacgggca ttacctctgt ttatccgccc aagaatatcg ttttccatac ccctgccgga
    10981 cgctggggca cgactgccgc tgccgccgtc agtacgcgcg aaggcctgct cgatttgacc
    11041 gacagtctgg acgaagccgc catcgacaaa tacagctaca cgcgcgacct ctatatgaaa
    11101 gtccgcgcac ggcagaccgg tgcaacacct gccgaaggta cggaagataa catcgacatc
    11161 gacgaattgg tcgaaagtgc cgaaaccggc gcggcagagc ccgccgttca cgaagattcc
    11221 gtatccgaaa cacaggcaga agcagcaggg gaagccgaaa cgcaacctgg aacacaaccc
    11281 taaagccatg ccgtctgaaa atccgtgttc ggacggcatt cccttccgtc ggaacggaca
    11341 gtccgacact acagattggg ctataatccc ttttttgcgc ataccgcgcg acagcaaagg
    11401 aaaaaccatg tacgaagtca accgcagcgt attcgtcctc atccctttag aacctttttg
    11461 gaactggctg caaaccctgc cgggcaacca cctcgacgga ctgaccctcg aagacatcca
    11521 agccgatgcc aattcctacc ttgtccgccc gtgcgaaacc gccgacgaag tatgggacga
    11581 aatcgaagcc cgtttcgaag acattttcgc cgccgaactt gccgactggt gcgaagacga
    11641 acgcgagtgg cccgcactcg atgccgacat tttcaacgaa tggttcgaca tccagctttc
    11701 taccgtcatc accgacctcg aacacgaacc gcttgcccgc gaagccttcc aacccatcaa
    11761 cctgaactga tgaagctgac cgtccgcaat taccatctcg acggctacgg tcatgtcaac
    11821 aatgcacgct acctcgaatt tctcgaggaa gcgcgctggg cgttttttga aaaacgcgga
    11881 ctgatgcacg agttggcagg cctcatactg attgtcgccc gcatcgacat ccgatacagc
    11941 cgtcccgccg tcgaaggcga cgtattgcag ttttcatgcc gcctgaaacc ccccggcatg
    12001 cgccgcattg tgctgaccca gacgattaca ctgccaaacg gcaaaaccgc cgcagaagcc
    12061 gacatcaccc tgatgcccgt ccacgccgcc acacaacgca ccgtcagcct gcccgccacc
    12121 cttgcccgcg cactggaagc cttgtccgaa tgaaaaaaat actcaccgcc gccgccgtcg
    12181 cactgatcgg catcctcctt gccaccgtcc tcatccccga cagtaaaacc gcgcccgcct
    12241 tctccctgcc cgacctgcac ggaaaaaccg tttccaacgc cgacctgcaa ggcaaagtca
    12301 ccctgattaa tttttggttt ccctcctgtc cgggttgtgt gagcgaaatg cccaaagtca
    12361 tcaaaacggc aaacgactac aaaaataaag atttccaagt cctcgccgtt gcccagccca
    12421 tcgatccgat agaaagcgtc cgccaatacg tcaaagacta cggactgccg tttaccgtca
    12481 tttatgatgc ggacaaagcc gtcggacagg cattcggcac acaggtttat ccgacttccg
    12541 tccttatcgg caaaaaaggc gaaattctca aaacctacgt cggagaaccc gatttcggca
    12601 aactctacca agaaatcgat accgcgctgg cacaatagcg atgccgtctg aagcgcaaaa
    12661 aaagcaggaa cgatgttcct gttttttata atggattaac aaaaaccggt acggcgttgc
    12721 cccgccccgg ctcaaaggga acgattccct aaggcgccca agcaccaagc gaaccggttc
    12781 cgtaccattt gtactgcctg cggcccgccg ccttgtcctg atttttgtta atccactata
    12841 gattatccgc cccgacacgt tcaggcggca ttcaaccttc cggcagattc tttttcacca
    12901 ccgcaggatt gcctgccgcc aaagagtagg gcggaatgtc ttttgtaatc accgcgcctg
    12961 cgcccacgac cgagccgcgt ccgacggtta cgcccgccat cacaatcacc ctgcgccccg
    13021 gccagacatc gtcctccaac gtaatcggac ggatttccgt gtagccctca aagcgttttt
    13081 ttcacggtca aacttgtggt tggtcgaata gagcaggcat tccggcccca tcatcacatt
    13141 tttgccgacc accagcccac ggcagatttc acagtttgcc ccgatgcccg agccgtcgcc
    13201 caaaaccgta tccggaaaca catacgcccc gcgtccgata tgcggcgaaa tccgccgcgc
    13261 cagaaaaccg agcacccgtc tgccgatgat gccgatgccg cgcatatgcg acggcggcaa
    13321 caccgaaccg gcaaaacgca gcagcaggaa aaagaatttt gatcccatca aacctccctc
    13381 aaaagaa
//
LOCUS       DAXFHF010000058        12828 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXFHF010000058 DAXFHF010000000
VERSION     DAXFHF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.058"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..641
                     /locus_tag="ACFIRM_001632"
     CDS             <1..641
                     /locus_tag="ACFIRM_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029221.1"
                     /translation="CTSTQVTSFVEGLSEGLVDVSEAFITTKAESYSAPSLQDKQIFI
                     NRVSERAQKNYKERRKKKEADLLNSASLLNESTSEALSALKNAIVIPKRPKRSLGKKY
                     KNGFHPVNTCADVEARWANGAMRDVRDIERLISRNVPLRRIGDAIQNMCMETNVSIRM
                     YRQECRSESSDFYDEMLEHHENFIGSSKSFASSLLPLSVTNFTTDMACTPEF"
     gene            726..1517
                     /locus_tag="ACFIRM_001633"
     CDS             726..1517
                     /locus_tag="ACFIRM_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC3029222.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            1668..2312
                     /locus_tag="ACFIRM_001634"
     CDS             1668..2312
                     /locus_tag="ACFIRM_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3029223.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            2331..4241
                     /locus_tag="ACFIRM_001635"
     CDS             2331..4241
                     /locus_tag="ACFIRM_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3029224.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            4263..4628
                     /locus_tag="ACFIRM_001636"
     CDS             4263..4628
                     /locus_tag="ACFIRM_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029225.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWHASWTFKTILALIP
                     ATAGYLFKWKQYRQRKTKP"
     gene            4740..6215
                     /gene="trpE"
                     /locus_tag="ACFIRM_001637"
     CDS             4740..6215
                     /gene="trpE"
                     /locus_tag="ACFIRM_001637"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC3029226.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTCLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            6299..7237
                     /locus_tag="ACFIRM_001638"
     CDS             6299..7237
                     /locus_tag="ACFIRM_001638"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3029227.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDDWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            7238..8098
                     /locus_tag="ACFIRM_001639"
     CDS             7238..8098
                     /locus_tag="ACFIRM_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC3029228.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            8115..9251
                     /locus_tag="ACFIRM_001640"
     CDS             8115..9251
                     /locus_tag="ACFIRM_001640"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC3029229.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            9298..9780
                     /locus_tag="ACFIRM_001641"
     CDS             9298..9780
                     /locus_tag="ACFIRM_001641"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3029230.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            9886..10934
                     /locus_tag="ACFIRM_001642"
                     /pseudo
     CDS             9886..10934
                     /locus_tag="ACFIRM_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            11514..12119
                     /locus_tag="ACFIRM_001643"
     CDS             11514..12119
                     /locus_tag="ACFIRM_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC3029231.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            12182..12544
                     /gene="ssrA"
                     /locus_tag="ACFIRM_001644"
     tmRNA           12182..12544
                     /gene="ssrA"
                     /locus_tag="ACFIRM_001644"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3632 a         3532 c         2863 g         2801 t
ORIGIN      
        1 cctgtacttc gactcaagta acttcttttg tcgagggact ctccgaggga ctcgtagatg
       61 tcagcgaagc ctttatcaca acaaaggcag aatcatattc tgccccgtct cttcaggata
      121 agcaaatttt cataaatcgc gtctcagaac gagcacaaaa aaattacaaa gaaagaagga
      181 aaaagaaaga agcagactta ctaaattcag catcgttatt aaatgaatca acatcagaag
      241 ccctatctgc ccttaaaaac gctatcgtaa ttcctaaacg ccctaaacgc tccttgggca
      301 aaaaatataa aaacggtttt catcctgtaa atacgtgtgc ggatgtggaa gctcggtggg
      361 caaacggagc aatgagagat gttagggaca tagaaaggct aataagccga aacgtccctt
      421 tacgtcgtat cggagacgcc attcaaaaca tgtgcatgga aaccaatgtg tccattagga
      481 tgtatcgcca ggagtgtcgt tcagaatcgt cagattttta tgacgaaatg cttgagcatc
      541 acgagaattt cataggatcc tccaaatcat tcgcatcatc acttcttccc cttagcgtta
      601 caaactttac aacagacatg gcgtgtactc ccgaatttta atctaatcac actccacaac
      661 tccctatcaa tataactgct acttctctga gtagtatcaa ccaaccctta acaaaggact
      721 aaaaaatgaa aaaagcactg cttgcactga ctattgccgc catctccggt actgctatgg
      781 cccagttgcc cgactttctg ggtaaaggcg aatataccgt ccgtacagac atctccaaac
      841 aaacgctgaa aaatgccgat ttgaaagaaa aacacaaagt acaaaaaaac atcggtttcc
      901 gtgccgatat gccgtttgac gatattcacc acggcatgcg tttcgaagta tcacacagcc
      961 gagacaaaaa agacatgtac gttgtgaccg aaagtactac taaaccattc ggcaaagacg
     1021 ttaaagaaaa acgcaccgac gtgtatgccg gttacaccta cacccaaccg ctcagtgaag
     1081 ccaccaaact gcgtgccggt ttaggtttgg gctatgaaaa atacaaagat gcggtagcta
     1141 atgagaaagg aacagtcagc accgagcgcg aagccttcta taccaaagcg cacgccgacc
     1201 tgacttccga tttgggcggc ggctggtatc tgaatccttg ggcagaggtt aaggttgacc
     1261 tggacgccaa attgaaacac aacgcaactg tagccggtgc ctcagccgac attaacgcaa
     1321 aaacacgcgg atggggcgtg ggcgtaggtg cgaatatcgg caaacaaatt accgataccg
     1381 tcggcatcga agccggcccg ttctacaaac accgtcactt caaagcatcc ggcagcttcg
     1441 ttttggacgg cgggaacatc cgagtcgatc ctaccaaaat caacgaatac ggtgttcgtg
     1501 tcggcgtgaa attctaatcg cggcgatatg ccccagacac aaaaccgcaa gccttttggt
     1561 ttgcggtttt tgatgttgta gcagacccta ataccgtctg aaacttttca gacggcattt
     1621 ttccaatcgg ttaaaatacc gcctcgacat tgtgttttgg aaaacccgtg cttgcttctg
     1681 tgattgattt tatcctgcat atcgaccaac acctgctcag cctgtcggcg caatacggtg
     1741 tgtggattta tgcgattctg tttttgattg ttttttgcga aaccggtctg attgttacgc
     1801 ccttcctgcc cggcgattcg ctcttgtttg ccgccggcgg gattgccgcg ctgggcggta
     1861 tggatattca tttgatggtt gcattattaa gccttgccgc catactcggc gacgcgctca
     1921 attttaccgt cggcaaatat ttcggcggca ggctgttcgc caaccccgat tctaaaatct
     1981 tccggtgcga atacctcggc aaaacccgcc gtttttacga aaaacacggc ggcaaaacga
     2041 ttatcatcgc gcgcttcgtg cccatcgtcc gcaccttcgc ccccttcgtc gccggtatgg
     2101 gaaaaatgca ctatgccaaa tttatccgct acaacatcat cggcggcttg ttgtgggtta
     2161 tcttgttttc ttacgcgggc tatttctttg ccaacttccc cgtcgtaaaa aacaatctgg
     2221 gcttggtgat gggcggcatc attattgttt ccgtcctgcc cggcatcatc gaaatcgccc
     2281 gcgccaaact tgcggcaaaa tccgaacgtt aattgttgga aattgacatt atgaatatat
     2341 tatccgtaga aaacgcttcc tttgccgtcg gacatgtcgc cctgctcgac aaaacgtcct
     2401 tccaactcga cagcggcgaa aaagtcggct tgatcggacg caacggcgca ggcaagtctt
     2461 cgtttttaaa aatcctcacc ggcgtgcaaa aactcgacga cgggcagatt attgttcaaa
     2521 acaatctcaa aatcgtttat gtgccgcagg agtccttttt tgataaggac gcaaccgtat
     2581 ttgatactgt tgccgaaggt ttgggcgaaa ttcgcgattt attgcgccgt tatcatcatg
     2641 tcagccatga gttggaaaat ggttcgagtg agctcttatt gaaagagctt aacgaattgc
     2701 aacttgaaat cgaagcgaag gacggttgga aactggatgc ggcagtcaag cagactttgg
     2761 gggaactcgg tttgccggaa aacgaaaaaa tcggcaacct ttccggcggt cagaaaaaac
     2821 gcgtcgcctt agcgcaggct tgggtacaga agcccgatgt attgttactg gacgaaccga
     2881 ccaaccattt ggatatcgac gcgattattt ggttggaaaa cctgctcaaa gcgtttgaag
     2941 gcagcttggt cgtgattacc cacgaccgcc gttttttgga caatatcgcc acgcgcattg
     3001 tcgaactcga tcgcggtatt ttgcgctcct atcccggctc gttctccaaa tacagcgaga
     3061 aaaaagcgca agagttggcg gttgaagccg aacacaaccg cctcttcgac aaattccacg
     3121 cgcaggaaga ggcgtggata cgcaaaggca tcgaagcgcg ccgtacccgc aacgaaggcc
     3181 gcgtgcgccg tttggaagaa ctgcgccgcc agcgtgccga acgccgcaat gtacaaggac
     3241 aggtcaactt caaactcgac agcggcaaga aaagcggcaa aatcatcgcc gaactggaac
     3301 acgcttcgtt tgcctatgac gacaaagtta tcatggataa attttccgcc atcttgcagc
     3361 gcggcgacaa aatcggctta atcggcccaa acggtatcgg caaaaccacc ttccttaagc
     3421 tgattttggg cgaattgcag ccgacctacg gcagaatccg tatcggcagc aagcaggaag
     3481 tcgcctattt cgaccagttc cgcagcgcct tgaatgaaaa cgatactgtg ttttacacgc
     3541 tcggacaggg caacgattac gtcgaagtcg gcggcaagaa aaagcatgtg atgagctatt
     3601 tggaagattt cctgttcccc cccgcccgcg cgcaaagtcc tgtttcttca ctctccggcg
     3661 gcgaacgcaa ccgcctttta ctggcaaaac tctttactcg tcccgccaat attttggttt
     3721 tggacgaacc gaccaacgac ttggacatcg acacccaaga attgcttgaa gatctgttgc
     3781 gcgattacca aggcacggta ttcctcgtct cgcacgaccg tatgttcctc gacaatgtaa
     3841 ttacccaaag cattgttttc gaaggacaag gccgtctgaa agaatacatc ggcggctatc
     3901 aggactatat cgacgcaaaa tcgcgggaaa ataaaattca gacggcatcc gcacccaaag
     3961 catccgatgt cgaacccgcc aaagaaaaac ccaaagccaa ccgtacggtc aaactttcct
     4021 acaaagaaca gcgcgaactc gacgccctgc ccgacgaaat cgccgccttg gaaaccgaac
     4081 aggcagaaat caacgcgcag ctttccgatc ccggaatttt caaagattac gaaaaagcag
     4141 gagcattgca aaaccgcgcc gaagaaatcg aaatgctgct tttggaaaag ctggaacgct
     4201 gggagctgct ggaaaccaaa cagaacggca atgccgtctg aaatcaaaca ggaagaaaaa
     4261 aaatgttcaa acgacctgaa gaaatcatcg tcctgatact tgccgtcctc tggattgccg
     4321 gtacgtattt tctcgccgcc ctgtttggag cggatgctta taccgtgtta aaaatcactg
     4381 cccttaccct gctttggtct gccgcatcat tcctcctttg gcaaaaaaac ctcagccggc
     4441 atatctggcc gctgctgctc ggctgcctga ctgcctgttg gtggccgtat ctgaatcaat
     4501 cggtcgcacc cggttcttta ccttggcatg catcatggac gttcaaaacc atcttagccc
     4561 tgattcccgc aaccgcaggt tatctgttta aatggaaaca ataccggcag cgcaaaacaa
     4621 agccgtaagc ccgtcttttt tcagacggca tctccggtca gactgtcaac aattccgata
     4681 aaatgttata ctcgcgcccg ttgccgcgcc gtccgaaacc gccttttggg aaagcagaaa
     4741 tgatcagcaa acaagaatac caagcccaag ccgcccaagg ctacaaccgc atcccgctcg
     4801 tgcaagaact ccttgccgac ttggatacgc cgctttccct ctatctcaaa ctcgccaacc
     4861 gcccctatac ctacctgctc gaatccgttg tcggcggcga acgtttcggc cgctattcct
     4921 ttatcggcct gccttgcagc cactatctca aagccggcgg caaacacgtc gatgtttatc
     4981 aaaacggcga aatcgtcgaa caatacgacg gcaatccgct accctttatc gaagctttcc
     5041 acaaccgttt caaaacaccc gaaatcccaa gcctgccgcg ctttaccggc ggcttggtcg
     5101 gctacttcgg ttacgaaacc gtctacaatt tcgaacactt cgcccaccgc ctgaaaaaca
     5161 ccgccaaagc caacccgctc ggcacgcccg atattttgct gatgctgtcg caagagttgg
     5221 cggtaatcga caatttgagc ggcaaaatcc acctcatcgt ttatgctgat ccctcgcagc
     5281 ccgacagcta cgaacgcgcc cgcgaacgcc tcgaagacat ccgcacccag ttgcgccaaa
     5341 gctgcgccat cccactttcc ctcggcagca aacaaaccca agcagtcagc gagttcggcg
     5401 aagaaccttt caaagcctgc gtcgataaaa tcaaagacta cattttcgca ggcgactgta
     5461 tgcaggtcgt ccccagccaa cgcatgagca tggaatttac cgacaatccg cttgccctct
     5521 accgcgccct gcgcacgctc aacccttcgc cttacctctt ttactacgat ttcggcgatt
     5581 tccacatcgt cggttcctcg cccgaaatcc tcgtccgccg cgaacgcgac gacgtcatcg
     5641 tccgccccat cgccggcacg tgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg
     5701 aacaagattt gttaagcgac gccaaagaaa tcgccgaaca cgttatgctt atcgatttag
     5761 ggcgcaacga tgtcggacgc atcagcaaaa caggcgaagt caaagtcacc gacaaaatgg
     5821 tgattgaaaa atactcccat gtgatgcaca tcgtttccaa cgtcgaaggc tgtctgaaag
     5881 agggcgttac caatatggac atcctcgccg ccaccttccc tgccggcaca ctctccggcg
     5941 cgccaaaagt ccgcgcgatg gaaatcatcg aagaaatcga accggagaaa cgcggcatct
     6001 acggcggcgc cgtcggcgta tggggcttca acaacgatat ggatttggca atcgccatcc
     6061 gcaccgccgt gattaaaaac aataccctct tcatccaaag cggcgcgggc gtggttgccg
     6121 actccgaccc aacttccgaa tggcaggaaa ctcaaaacaa agcccgcgca gtggttcggg
     6181 cggcgcagat ggtgcaggaa gggttggata aataaatttc agacaacctt tgcagatgaa
     6241 aaaaaggtcg tctgaaaatc acaaaacaac atcagagata cttacataaa aaatcacaat
     6301 gcaattcaca tcattagaaa tttgtgccgg agcaggcgga caggctttgg gcttagaaag
     6361 ggcaggcttt tcccatgttg cgttaatcga aatcgaaccg tcggcttgtc aaaccctacg
     6421 tttaaaccgt cctgattgga acgttattga aggagatgtc cgcttgtttc agggcgaagg
     6481 atatgacggt attgatttat tggccggcgg cgtgccttgc cctccttttt ccaaagctgg
     6541 aaaacaactg ggaaaagatg atgaacgcga tttattccct gaggctattc gtctagctaa
     6601 ggaaactgac cctaaagcca tcatgttaga aaatgtgcgg gggttacttg acccaaaatt
     6661 tgaaaattat cgcaaccata ttacagaaca atttgccaag ttgggttacc ttggacaatg
     6721 gaaactactt tatgctgccg attacggagt atcgcaactc agacccaggg ttttatttgt
     6781 tgcattgaaa aacgaataca ccaatttttt caaatggcct gaaccaaatt cggaacaacc
     6841 gaaaaccgtt ggagaactgc tttttgactt aatgtcagaa aataactggc agggggcgca
     6901 taactggcgt ttgaaggccg cgcagattgc accgaccttg gtcggcggtt caaaaaaaca
     6961 cggcggtgct gacttggggc ctacacgatc caaacgcgca tgggcggagt tgggtgtgga
     7021 tggttcaggt ttgtgggata gtgcgccgcc tgaagacttt tcgggaatgc ctagattgac
     7081 tgtccgaatg acggcgcgta tccaaggatt tcctgatgac tggcaatttt ttggtaaaaa
     7141 aacgcctatg taccgccaaa tcggcaacgc ttttcccccg ccggtagccg aagcggtggg
     7201 caggcagatt atcaaagcct taaaaaagga aaattgaatg aatccgcttt ttacccaaga
     7261 acgcaggatt ttccataaga aattattgga tggcaatatt cttgcaacca acaatagagg
     7321 cgtagtcagc aatgcggatg ggagcaatac ccgaagtttc aatatagcca aggggattgc
     7381 cgacttactg cattcggaaa cggtttcgga aagattaccc ggtcagacat cgggaaatgc
     7441 ttttgaagca atatgcagtg agtttgtcca atctgctttt gagaaattgc agcatatcag
     7501 acccggcgac tggaatgtta agcaggtcgg ttctcgcaac cgtctggaaa tcgcacgtta
     7561 tcagcaatac gcacatctga cagccttagc caaggctgcc gaagaaaacc ccgaattggc
     7621 tgccgccttg ggcagtgatt acaccattac tcccgatatt atcgttacca gaaatttgat
     7681 tgcagatgca gaaatcaacc gtaatgaatt tttagttgat gaaaatattg ccacatatgc
     7741 cagtttgcgt gcgggtaatg gcaatatgcc gctgctccac gccagtatct cttgtaaatg
     7801 gacgattcgc agcgacaggg ctcaaaatgc ccgttctgaa ggattgaatt tggttagaaa
     7861 cagaaaaggc agactgccgc acattgttgt cgtaacggca gaaccaacgc caagccgcat
     7921 ttcatctatt gccttgggta caggggaaat agattgcgtt taccattttg ccttatatga
     7981 gctggaacaa attttacaat cattgaatta tgaagacgct ttggatttgt tttacatcat
     8041 ggtcaatggt aaacgattaa aagatatttc cgaccttccc ttagatttgg cagtttaaac
     8101 agaattattc cactatgaac acacccccca tccttcctcc cgccatgctc ggcatcctcg
     8161 gcggcggaca attaggcaga atgtttgccg ttgccgctaa aaccatgggc tacaaagtaa
     8221 ccgttctcga tcccgacccg aatgcgccgg cggcggaatt tgccgaccgc catttgtgcg
     8281 cgccgtttga cgaccgggcc gcgttggacg aattggcaaa atgcgcggcg gttacgaccg
     8341 aatttgaaaa cgtcaatgcc gacgcgatgc gctctctggc aaagcatacc aacgtttccc
     8401 ccagcggcga ctgcgtgtcc attgcacaaa accgcattca ggaaaaagcg cggatacgca
     8461 aagcaggctt gcaaaccgcg ccgtatcagg cggtttgcaa ggccgaagac attactgaag
     8521 caagcgcgca atttttgccc ggcatcctga aaacggctac gttgggctac gacggcaaag
     8581 gtcaaatccg cgtcaaaacg ttggacgaac tcaaagccgc gtttgccgaa cacggcggcg
     8641 tggattgcgt tttggaaaaa atggtggact tgcgcggcga gatttccgtg atcgtatgcc
     8701 gtctgaacga tgaaaacgtg caaaccttcg accccgccga aaacatccac gaaaacggca
     8761 tcttggctta ttccatcgtc cccgcgcggc tgagtgccga cgtgcagcaa caggcgcggc
     8821 agacggcgca acgcttggcg gacgaattgg attatgtcgg cgtattggcg gtagaaatgt
     8881 ttgttgtcgg cgacacacat gaattgctcg tcaatgaaac cgccccgcgc acgcacaatt
     8941 ccggccacca tacgatagat gcctgcgccg cagaccagtt ccaacagcag gtgcgcatta
     9001 tgtgcaacct gccgcccgcc gacaccaaat tattatcccc ttgctgtatg gcgaatattt
     9061 tgggcgacgt ttggcaggaa gatggcggcg aaccggattg gctgccgttg caaagccggc
     9121 cgaatgcaca cctgcaccta tacggaaaaa aaaccgcaca gaaaggtcgg aaaatgggac
     9181 actttaccgt tttgaccacc gattcggaca ccgcatttca agaagcaaaa aaactgcatc
     9241 agtccctata atccgaccaa cggcacagat gccgtctgaa gccgccgaca taccgccatg
     9301 agcctgctga cactgttacg ccccgccacc gtgcaagact gcaaagacat cttcaaagtc
     9361 cacctgcact ccgtccaata cacctgcatc ctcagctaca acgaacacgc cctaaaagta
     9421 tgggaaggct tgttaaacac agaaagctac ctgccgacca tttccgatcc cgacaaagca
     9481 ctttgggttg ccgaatacaa aggcaacata cagggatttt tccaaatcga ctgccaagaa
     9541 gcacaactcg atgcgcttta cgtccacccc ctgttccaca atctcggact gggcactgcc
     9601 ctgctccgtc aggcagaaac catcgcccac aaatccggtt tgagctttct gaaactctac
     9661 gcatctctca actccgtccc gttttacctt ctcaaccgct acgaatcgct cggttcggca
     9721 gtattgcagc ttgaccccag catcaaaatc aaatgcgaac tgatgcgcaa acacctctaa
     9781 tcccgcctta taaccaaaac tgataatctt caaatcaaac attatttcca caagcgcaga
     9841 cagccgtata aagttgcccc atctaccaac cgacaggaca agcatatgaa aacctacgca
     9901 caggcactct ataccgcagc cctgcttacc gcctgcagcc ccgcagccga ttcaaaccat
     9961 ccgtccggac aaaatgcccc ggccaatacc gaatccgacg gaaaaaacat taccctgctc
    10021 aatgcctcat acgatgtgac acggtatttt tacaaagaat acaaccactt atttatcaaa
    10081 acataccaat ccgaacaccc cggcacatcc gtcagcatcc aacaatccca cggcggcttc
    10141 agcaaacagg cattatccgt agccaacggc cttcaagccg atgtcgtaac catgaaccaa
    10201 tcttccgaca tcgacctgct cgaaaaaaag gactggtaga aaaaggctgg caacaagccc
    10261 tccccgatca cgccgcaccc tacaccagca ctatggtttt ccttgtccga aaaaacaacc
    10321 ccaaacagat ccgcgattgg aacgaccttg ccaaagacgg cgctaacatc gtcatcgcca
    10381 agacctcggg caacggacgc tacgccttcc tcggcgcata cggttacggt ctgaaagcca
    10441 acaacggcaa cgagcaggaa gcccaaaaac tcgtcgcatc catcctcaaa aacacacccg
    10501 tttttgaaaa cggcggacgc gccgccgcca ccaccttcac acaacgcaac atcggcgacg
    10561 tactcatcac ttttgaaaac gaagccaact acgtcagcaa aaaactgacc caaggacagt
    10621 ttgaaatcgt ctatccgagc tacaccattt ccgccgaaag ccccgttgcc gtcgtcaaca
    10681 gcgtcgtcgc gaaaaaaggc acgcaaaaaa ccgcccgcgc ctatctcgaa tacctttgga
    10741 gcgaaccggc gcaagaactc gccgcctcac tctacctgcg tccccgcaac cccgaagtat
    10801 tggcacgaca caaagccgac ttccccgact tagacacctt ccctcccgaa gaaaaattcg
    10861 gcggatggga caacatcatg aaaacctact tcgccgacgg cggcgtattc gaccgcctga
    10921 ccgcacaaaa atgaccatta tgccgtctga accgcctttc agacggcatt tcacacatca
    10981 aaacagaaaa ccgcacccaa aatcatcatc acaagacaca acggcaaacc ggatacaaaa
    11041 cccaacaccg ccgccaaaat gccgtctgaa gccgtcggcg caaccatcct tatctggggc
    11101 gcgcctcatc aaaggggcgc ataactttcc ttaaccatcc cttttgacag ccaaataaac
    11161 agtgcttttg cctgtcagaa acaaaaccaa tattttgcgg ttcgtatcgg aacatttata
    11221 aaacatcaga tttccgtgtt tcgcgtttcc ggcaattcaa gattagcaaa accattaacc
    11281 tgcttcgcaa ggcaggtttt taaaacttta tcaggcgttc gagcatatcg gttcgaccga
    11341 cccgcatttc cggcaatcaa cactgccgac ggttgccgca tttctttttg atatacaatc
    11401 acactttcaa aaaacatacg acggcaacaa aatcatcctc cccccgcttg gcaaacatcc
    11461 ggcaaattaa aaccccgccc gaaacagcca tcggacaaat aacaggaaaa aatatgaata
    11521 ccgacttcca aaagatcctc aaacaactgg gcaaacaggc caaaaaagaa gcggcagaaa
    11581 aacaggaagc cgaaaaaaac aagcaaaaac aagaatatga tttggatttt tcacaagcag
    11641 tcggacaagt ctccccatta aaaaaccggc agcaatatta tgcgcagtcc gacaaaaccc
    11701 ccatcaaagc ccgtcccaaa gacaaccgca ccgacgaaga aaactacttc tacataggca
    11761 gcacatacaa cgacccaccc gccagcttct gcaaaaacgg acagggaaaa aacgacatcc
    11821 aacgcctgaa aaacggatac tatcccgtcg ttaccgatgt cgacctgcac ggctacaccc
    11881 aagaagaagc acaaaaagtc ctcaacgaat tcatcgcatt cacccaaaaa cgcggcgtat
    11941 gcggcgaaat catccacggc agcggattag gttccaaagg ctacaaaccc gttctgaaaa
    12001 acatgacccg aaactggctg atgcaacacc ccgacgtact cgcctacgtc gaaccccggg
    12061 aaggcaacga cggctgcgtc cgcatcctgc tcaaacgcaa attaaggcag caagactgac
    12121 aaatttctcc gacagacttg gcagcaccgc acaatatccg tataatttgc aaaccatttc
    12181 tgggggcgac cttggtttcg acgggggttg cgaagcagat gcgggcatac cggggtctca
    12241 gattcccgta aaacactgaa ttcaaatagt cgcaaacgac gaaacttacg ctttagccgc
    12301 ttaaggctag ccgttgcagc agtcggtcaa tgggctgtgt ggcgaaagcc accgcaacgt
    12361 catcttacat tgactggttt ccagccgggt tacttggcag gaaataagac ttaaggtaac
    12421 tggtttccaa aaggcctgtt ggtcggcatg atggaaataa gattttcaaa tagacacaac
    12481 taagtatgta gaacgctttg tagaggactt tcggacgggg gttcgattcc ccccgcctcc
    12541 accatgccct attttcagca gtatcctgca acacaaaaaa tccttgaact tcaaaagttt
    12601 caaggctttt ttattgcctt gaatatgatg gcgtatgctt gagttttcgc aatttatggg
    12661 gtaacttttg gggtaacttt tcaggaatca ccctaaaaaa tatcttaaaa atacaaaagc
    12721 catcacgcat tttttaaaaa ggttacccaa aacttaaatg aagcctttat ttataaggct
    12781 tgcagctttc caaacaagtt acccccaaac ggataaaaaa tgccgaag
//
LOCUS       DAXFHF010000059        12486 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXFHF010000059 DAXFHF010000000
VERSION     DAXFHF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12486
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.059"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            209..1552
                     /locus_tag="ACFIRM_001645"
     CDS             209..1552
                     /locus_tag="ACFIRM_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC3029232.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1555..2787
                     /locus_tag="ACFIRM_001646"
     CDS             1555..2787
                     /locus_tag="ACFIRM_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC3029233.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYVLYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2780..3556
                     /locus_tag="ACFIRM_001647"
     CDS             2780..3556
                     /locus_tag="ACFIRM_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC3029234.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3556..4182
                     /locus_tag="ACFIRM_001648"
     CDS             3556..4182
                     /locus_tag="ACFIRM_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC3029235.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4186..4779
                     /gene="nqrE"
                     /locus_tag="ACFIRM_001649"
     CDS             4186..4779
                     /gene="nqrE"
                     /locus_tag="ACFIRM_001649"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC3029236.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4793..6010
                     /gene="nqrF"
                     /locus_tag="ACFIRM_001650"
     CDS             4793..6010
                     /gene="nqrF"
                     /locus_tag="ACFIRM_001650"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC3029237.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6527..7582
                     /locus_tag="ACFIRM_001651"
     CDS             6527..7582
                     /locus_tag="ACFIRM_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC3029238.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7608..7823
                     /gene="nqrM"
                     /locus_tag="ACFIRM_001652"
     CDS             7608..7823
                     /gene="nqrM"
                     /locus_tag="ACFIRM_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC3029239.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7947..8525)
                     /gene="grpE"
                     /locus_tag="ACFIRM_001653"
     CDS             complement(7947..8525)
                     /gene="grpE"
                     /locus_tag="ACFIRM_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC3029240.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVARKEA"
     gene            8708..9526
                     /gene="cysE"
                     /locus_tag="ACFIRM_001654"
     CDS             8708..9526
                     /gene="cysE"
                     /locus_tag="ACFIRM_001654"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC3029241.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9730..11241)
                     /gene="ubiB"
                     /locus_tag="ACFIRM_001655"
     CDS             complement(9730..11241)
                     /gene="ubiB"
                     /locus_tag="ACFIRM_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC3029242.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11276..11736)
                     /locus_tag="ACFIRM_001656"
                     /pseudo
     CDS             complement(11276..11736)
                     /locus_tag="ACFIRM_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11733..11879)
                     /locus_tag="ACFIRM_001657"
     CDS             complement(11733..11879)
                     /locus_tag="ACFIRM_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029243.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11896..12234)
                     /gene="erpA"
                     /locus_tag="ACFIRM_001658"
     CDS             complement(11896..12234)
                     /gene="erpA"
                     /locus_tag="ACFIRM_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC3029244.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12367..>12486)
                     /locus_tag="ACFIRM_001659"
     CDS             complement(12367..>12486)
                     /locus_tag="ACFIRM_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3029245.1"
                     /translation="GIINVISANEAYPAFEINLNDLTDDVEIIGRVEWFGRTV"
BASE COUNT         3086 a         3489 c         3031 g         2880 t
ORIGIN      
        1 gtacagaccc ttatcaaatc aatatccccc ttccgacaat taaaaacggc ttccgcaagg
       61 aaaaaagcgg caaatctgac aaacaggtac aattcctgat aaaatctaac actttgctac
      121 attaatttat tcaatgcttt tgatgccacg cgacacggta tcaaaaacac gccgttttta
      181 ccttaaccat cacaagtaat gcaaatctat gattaaaatc aaaaaaggtc taaatctgcc
      241 catcgcgggc agaccggagc aagtcattta tgacggcccg gccattaccg aagtcgcgtt
      301 gcttggcgaa gaatatgtcg gcatgcgccc ctcgatgaaa atcaaggaag gtgaagccgt
      361 caaaaaaggc caagtgctgt ttgaagacaa aaagaatccg ggcgtagtat ttactgcgcc
      421 ggcttcaggc aaaatcgccg ctattcaccg tggcgaaaag cgcgtacttc agtcagtcgt
      481 gattgccgtt gaaggcaacg acgaaatcga gttcgaacgc tacgtacctg aagcgctggc
      541 aaaattgagc agcgaagaag tgcgccgcaa cctgattcaa tcaggcttat ggactgcgct
      601 tcgcacccgt ccgttcagca aaatccctgc cgtagatgcc gagccgttcg ccatcttcgt
      661 caatgcgatg gacaccaatc cgctggctgc cgaccctacg gtcatcatca aagaagccgc
      721 cgaagacttc aaacgcggcc tgttggtatt gagccgcctg accgaacgta aaatccatgt
      781 gtgtaaagca gcaggcgcag acgtgccgtc tgaaaatgct gccaatatcg aaacacatga
      841 atttggcggc ccgcatcctg ccggcttgag tggcacgcac attcatttca tcgagccagt
      901 cggcgcgaat aaaaccgtgt ggaccatcaa ttatcaagac gtgattgcta tcggacgttt
      961 gttcgtaaca ggccgtctga ataccgagcg cgtggttgcc ttgggcggcc tgcaagtcaa
     1021 caaaccgcgc ctcttgcgta ccgttttggg tgcgaaggtg tctcaactta ccgccggcga
     1081 attggttgac gcggacaacc gcgtgatttc cggttcggta ttgaacggtg cgattgcaca
     1141 aggcgcgcat gattatttgg gacgctacca caatcagatt tccgttatcg aagaaggccg
     1201 cggcaaagag ctgttcggct gggttgcgcc gcagccggac aaatactcca tcacgcgcac
     1261 cactctcggc catttcctaa aaaacaaact cttcaagttc acgacagccg tcaacggcgg
     1321 cgaccgcgcc atggtaccga tcggcactta tgagcgcgta atgccgttgg acatcctgcc
     1381 taccttgctt ttgcgcgatt taatcgtcgg cgataccgac agcgcgcagg ctttgggttg
     1441 cttggaattg gacgaagaag acctcgcttt gtgcagcttc gtctgcccgg gcaaatacga
     1501 atacggcccg ctgttgcgca aagtgctgga aaccattgag aaggaaggct gattatgggc
     1561 ttaaaacatt ttctggaaaa aatcgaaccg cacttcctgc cgggcggcaa acatgaaaaa
     1621 tggtatgtcc tctatgaagc tgcggcgacg attttctata catccggcgc ggtaacgcgc
     1681 aaagcggcgc acgtccgcga cgcgctcgac tccaaacgca tgatgatttt ggtgtggctg
     1741 gctttgttcc ccgccatgtt ttacggcatg tacaacgtcg gcgcacaggc attcggtgcc
     1801 ttaacgcccg atttgctgca acaaagcatc gccaacgact ggcattacgc ccttgccaac
     1861 gctttgggca tcaatatgtc gcctgaagcg ggcgtgttgg gcaaaatgct gttcggcgcg
     1921 atttacttcc tgccgattta cgcgaccgta tttattgtgg gcggcttctg ggaagtcttg
     1981 ttcgcatccg tacgcaaaca cgaaatcaac gaaggtttct tcgttacttc gattctgttt
     2041 gccttaatcg ttccgcccac gctgccgctg tggcaggcgg ctttgggtat ttctttcggc
     2101 gttgtggttg cgaaagaagt attcggcggt acaggtaaaa acttcatgaa ccctgcgctg
     2161 gcaggccgcg ccttcctgtt cttcgcctac cccgccaact tgagcggcga tgcggtttgg
     2221 acggcggttg acggctattc cggcgcaacc gcgctggcgc aatgggcggc acacggtgca
     2281 gacggtctga aaaacgccgt aaccggtcaa accatcactt ggatggacgc gtttatcggc
     2341 aaactgcccg gctccatcgg cgaagtctcc actttggcac tcttaatcgg cggcgcgttt
     2401 atcgtgtttg cccgcatcgc ttcttggcgc attattgccg gcgtgatgat cggtatgatt
     2461 gcgatgtctt cgctgtttaa cttcatcggt tctgacacca acgctatgtt tgctatgcct
     2521 tggtactggc acttggtggt cggcggcttc gccatcggta tgctgtttat ggcgaccgac
     2581 cccgtttccg cttcctttac caatgtcggc aaatggtggt acggcgcgct tatcggcgta
     2641 atgtgcgtgt taatccgcgt ggccaatccg gcttaccccg aaggcatgat gttggcgatt
     2701 ctgtttgcca acctgtttgc cccgattttc gactatttcg tcgcacaagc gaacatcaaa
     2761 cgcagaaagg cgcgcagcaa tggctaagaa attcgataaa gacagcttca gcggcacgct
     2821 gattgtcgtg ttggcggtca gcctgatttg ctcggtcatc gttgccggtg cggtcgtcgg
     2881 cttgaaaccc atccaagaaa aacaaaaact ccaagacaaa caaggctata ttttgagcgt
     2941 ggccggtttg atggataagg acaccgacat cggtaaaacc tttgccgagc gtatcgagca
     3001 acgcgtggtc gatttggcga ccggcgaata tgtgaaagac gcgccgaaag acttcagcgc
     3061 gcgcatcgca ggcaaagacc ccgcgcaaag catccgcatc aaacctgaag acgatttggc
     3121 aggcatcaaa agccgcgcca aatacaccga ggtttatttg gtaaaaggcg aagacggcaa
     3181 aatcgggcaa atcatcctgc cgatgcacgg caacggtttg tggtcggtga tgtacggctt
     3241 cgtcgccatc cgacccgacg gcaacaccat caacggcatt acctactacg aacaaggcga
     3301 aaccccgggc ttgggcggcg aaatcggcaa tcctttgtgg cagcaaaaat tcgtcggcaa
     3361 aaaattgttt gacggacaag gcaaactcgc cctgcacgtg ggcaaaggcg cgggttcgga
     3421 caaagaacac ggcgtagacg ccctctccgg cgcgtcgctg acttccaaag gcgtgcaagg
     3481 ttcgttcgcc tactggttcg gcgaaaacgg ctatatcccc tacctgaaca aattgaaatc
     3541 agcaggagtg caataatggc tgatatgaaa cgcttgaaac atttgatgtt ctcacccttt
     3601 atcgacaaca acccgattgc cttgcaggtt ttgggtattt gttcggcttt ggcggttacc
     3661 accaaacttc agacggccat cgtaatgggt atttccgtag ctttggtaac cggtttttcc
     3721 agcttcttca tttcgctggt gcgcaactac atccccaaca gcatccgcat catcgtgcaa
     3781 atggcgatta tcgcgtcgct ggttacgctg gtcgaccaac tgcttcaggc atttgcctac
     3841 gaattgtcca agcagctttc cgtattcgtc ggcctgatta ttaccaactg tatagtgatg
     3901 ggccgtgccg aagcatttgc gatgaaagag ccgccgctgg aaagcctgat cgacggcatc
     3961 ggcaacggtg cgggctacgg gatgatgctg cttgtcgtcg ccaccgtccg cgaactgatc
     4021 ggctcaggca aacttttagg ttataccgtc tttcaaaccg tacaggacgg cggctggtat
     4081 cagaccaacg gcctcttcct actcgccccc agcgcgttct tcatcatcgg ctttttgatt
     4141 tggggactgc gtacttggaa acccgaacag gcggaggaat aagctatgga acactatttg
     4201 agcctcttca tcaaatccgt cttcattgaa aatatggcgc tatccttctt tttgggtatg
     4261 tgtacgtttt tggcggtatc caaaaaagta tccaccgcat tcggtttggg tgtggcggta
     4321 attttcgtac tcgggctgtc cgtccccgcc aaccaattgg tttactcgct gctcaaagac
     4381 ggcgcgattg tcgaaggcgt ggatttgacc tttttgaaat tcatcacctt tatcggcgtg
     4441 attgccgctt tggtgcagat tttggaaatg ttcttggaca aattcgtccc cgccctctat
     4501 aacgcgctgg gcatctacct gcctctgatt accgtaaact gcgcgatttt cggcgccgta
     4561 tcgtttatgg cgcaacgcga atacgacttc ggcgagtccg tcgtgtacgg cttcggcgcg
     4621 ggtttgggct ggatgttggc gattgtcgct ttggcgggca ttaccgaaaa aatgaaatat
     4681 tcggacgctc ccaaaggcct caaaggcttg ggcattacct ttatcgccgc cggcctgatg
     4741 gcaatggcgt ttatgtcgtt ctccggcatc cagttataag aaagggaccg gtatggagat
     4801 tattttaggt atcgtgatgt ttaccgtcat cgtcttagct ttggcactga tgattctgtt
     4861 tgccaaatcc aagttggtca gcgaaggcga catcaccatc aaagtcaatg atgaaaaaga
     4921 actgactatg cccgccggcg gcaaactctt gggcgcgctt gccagccaag gcatctttgt
     4981 cccctccgcc tgcggtggcg gcggttcgtg cggacaatgc cgcgttgtcg tgaaaagcgg
     5041 cggcggcgac attctgccga ccgagctgtc ccacatcagc aaacgcgaag cacgcgaagg
     5101 ctgccgtttg tcctgccaag tcaatgtcaa aaccgacatg gacatcgaag tacccgaaga
     5161 agtgttcggc gtgaaaaaat gggaatgcac cgtcatttcc aacgacaaca aagcaacgtt
     5221 cattaaagaa ctcaagcttg ccattcccga aggcgaagaa gttcctttcc gcgccggcgg
     5281 ctacattcaa atcgaagccc cgccgcacac cgttgcctac aaagacttcg acattcccaa
     5341 ggaatatcac gaagactggg acaaatacaa tctgtggcaa tacgtttcca aagtggacga
     5401 gccgattttg cgcgcctact ctatggcttc gtatcctgaa gaaaaaggca tcattatgct
     5461 gaacgtgcgt atcgccacgc ctcccccgcg cgtacccgat gcgcctccgg gacaaatgtc
     5521 gtcctacatc tggtcgctca aacccggcga caaagttacg atttccggcc cgttcggcga
     5581 atttttcgcc aaagacaccg atgccgaaat ggtatttatc ggcggcggtg cgggtatggc
     5641 cccgatgcgt tcccacattt tcgaccagtt gaaacgtttg cactccaaac gcaaaatcac
     5701 cttctggtac ggcgcacgct ccaaacgcga aatgttctat gtcgaagact tcgaccaact
     5761 cgcggcagaa ttcccgaact tcacttggca cgtcgccctg tccgacccat tgcctgaaga
     5821 caactgggac ggctacacgg gcttcatcca caacgtggtt tacgaaaacc acctgaaaaa
     5881 ccacgaagca ccggaagact gcgaatttta tatgtgcggc ccgccgatta tgaaccagtc
     5941 cgtcatcaaa atgctcaaag acttgggcgt ggaagacgaa aacatcctct tggacgattt
     6001 cggcggttaa atattcggct tcaaacacaa aaggaagaaa ccccggtttc ttcctttttt
     6061 atccgatgcc gtccgaacac ccttcagacg gcatccgccc atacccgtat caatatcgtt
     6121 tttttaccct gcggcggatg tgcggctaaa tattttgacg aaaaaacaaa gaagtatact
     6181 tctttttagt tatggttgat tccataacta aaaaagtatc gatcaaccca ttttacgagg
     6241 tacggataat gtctgacagc tttgaaaaca gccccgaata cgatgattgg attgaatcgg
     6301 gcggtcgtga tgaagattac gaatactact acaacaaatg gcaacgcagg acgcgctgac
     6361 cattaaaccc tgacaaaccg gtttgtaaaa caccggtttg ttttttatta ccgccaccca
     6421 aacgacgggc gatgccgccc gaacgccctt caaacggcat cggcatagaa acataccggc
     6481 cgttttgcga tataattcaa gccatttgcc aaaaccgtca aacactatgc cgtctgaaac
     6541 acgcctgccg aaccttatcc gcgccttgat atttgccctg ggtttcatct tcctgaacgc
     6601 ctgttcggaa caaaccgcgc aaaccgttac cctgcaaggc gaaacgatgg gtacgaccta
     6661 taccgtcaaa tacctttcaa ataatcggga caaactcccc tcccctgcca aaatacaaaa
     6721 gcgcattgat gatgcgctta aagaagtcaa ccggcagatg tccacctacc agaccgattc
     6781 cgaaatcagc cggttcaacc aacacacagc cggcaagccc ctccgcattt caagcgattt
     6841 cgcacacgtt accgccgaag ccgtccgcct gaaccgcctg actcacggcg cactggacgt
     6901 aaccgtcggc cctttggtca acctttgggg gttcggcccc gacaaatccg ttacccgtga
     6961 accgtcgccg gaacaaatca aacaggcggc atcttatacg ggcatagaca aaatcatttt
     7021 gcaacaaggc aaagattacg cttccttgag caaaacccac cccaaagcct atttggattt
     7081 atcttcgatt gccaaaggct tcggcgttga taaagttgcg ggcgaactgg aaaaatacgg
     7141 cattcaaaat tatctggtcg aaatcggcgg cgagttgcac ggcaaaggca aaaatgcgca
     7201 cggcgaaccg tggcgcatcg gtatagagca acccaatatc atccaaggcg gcaatacgca
     7261 gattatcgtc ccgctgaaca accgttcgct tgccacttcc ggcgattacc gtattttcca
     7321 cgtcgataaa aacggcaaac gcctctccca catcatcaat cccaacaaca aacgacccat
     7381 cagccacaac ctcgcctcca tcagcgtggt ctcagacagt gcaatgacgg cggacggttt
     7441 atccacagga ttatttgttt taggcgaaac cgaagcctta aggctggcag aacaagaaaa
     7501 actcgctgtt ttcctaattg tccgggataa ggacggctac cgcaccgcca tgtcttccga
     7561 atttgccaag ctgctccgct aaacatacaa accgattagg aaacaccatg aaaaccctgc
     7621 tcctcacctt cggcatcttc ctgaccgtca tcatcggtat ggcggtcggc tatattttct
     7681 ccaaacgcac catcaaaggc agttgcggcg gcattaccgc tttaggtatg aaaaaaatgt
     7741 gcgactgcga cacaccttgc gacaccctgc aaaaaaagct ggatgaagaa aaacaggcag
     7801 gcggcatacg ggttgaccgt taacgccgaa acaaaatgcc gcctgaagcc cggccggcgg
     7861 gttcagacgg cattgccgct ttacttcgat cggtttatac gcaaaacgcg cttcaggaaa
     7921 taaatcagat tattccccag acgccttcag gcttccttcc gtgccaccgt aaccatagcc
     7981 gggcgcaaca cacggtcgga cagggtataa cccttcttca tcacaccaac cacggtattg
     8041 ggttcctgct cactggccac cgcctgcatc gcctgatgga tattcggatc gagcttatcg
     8101 cccgctttag gattgatttc cttgatttgc gtggcatcaa acgccttctg caactcattc
     8161 aaagtcatct gcacgcccat tttcagcgca tcaaaattac cgctctgatc caaaagcgcc
     8221 atttccagat aatccttgac cggcaacatt tccacggcaa acttctgtcc ggcgaacttg
     8281 tgcgtatcgg caatttcctg ctggtggcgg cggcgcaggt tttgctcgtt tgccaaagcg
     8341 cgcagttgtt cgtctttcaa ctgcgcttcc agctcggcaa tccgcgcctg caaatcctca
     8401 taagccggct cggcggcagc ctgttcctgt acaccgtccg catttcctac tgtctcgacg
     8461 gtttccaccg cctccacatt ttcaaccgct tcttcactgt tttgctgctg tgtctgttcg
     8521 ctcatatcgt atccctcaaa acaaattgga aatcaaaatc cggttattac ccgagcaaga
     8581 taaggacatt ttcaagaaac ttcaagcaaa ggcaggaaat ttcacatccg ccgccgaata
     8641 ccctaccgca aaaaccatat aaacggtaag ataccgccga ccttatgctt ttcagacggc
     8701 atccattatg aaaaaaaacc atttgaacac aaccggtttc gacctctggc acaccatccg
     8761 cgaagaaacc gcggccgctg ccgccgccga accgatgctg gcaagttttt tgcaccaaac
     8821 cgtgttgcgc cacgagtccc tcggctccgt ccttgcctac cacctttcca gcaaactcgg
     8881 cagcccgatt atggacgtgc gcgcgctgtt tgaaatttac cagcaggcat tgggcagcga
     8941 cacccaaatc agcaaatgtg tcgaagcaga cttaaaagcc atctacgaac gcgatcccgc
     9001 ctgcgacgaa tattcgctgc cgcttttata tttcaaaggc ttccacgcga ttcaggcaca
     9061 ccgcatcaac caccggctgt atctcgacgg acgcaaaacg ctggcgtatt tcctacaaaa
     9121 ccgtatgtcc gaagtattcg gcgtggacat ccatcccgcc gcccgtttag gatacgggct
     9181 gatgctcgac cacgccaccg gctttgttgc cggagaaacc gccgtgttgg gcaacaatat
     9241 ttcgattttg cacggcgtaa cgctcggcgg ttcgggcaaa gaaggcggcg accgccaccc
     9301 caaaatcggc gacggcgtga tgatcggcgc aaacgcctcg atattgggca atatccgcat
     9361 cggcagcaat gccaaaatcg gcgcgggcag cgtcgtggtt tcagacgtgc cgccgtccat
     9421 cacggttgtc ggcgtacccg ccaaacccgt ggcgcgatcg ctcaaaaccc cgtcggcgga
     9481 tatggatcaa aatatccagt ttgccgaaat cgactttatg atttgaatat ccgcaccaat
     9541 gccgtctgaa acgccaaacg ggcttcagac ggcattgccc gcttcaagcc tgcgccttaa
     9601 tatgccgccg atgccggcac ttatcagtcg gacaaaatgc cttttattcg atacgcccga
     9661 ttcccacgca accggaaata accggtttcc ttgtaaacgg gcggagtcgg gcaatgattc
     9721 ggacaaagcc tatttcaaaa gcaaaatcag cagcaaaaca accgctatcg ctgccagcca
     9781 caggctttgc cgctgctgca ctttgaccaa atggatatag gcatcgcgca tttcctgccg
     9841 gcgcgcctca tcgaccaagg cattgatttt gcgcggcaga gaagggatga tttgcgccca
     9901 gtctggggct tcgtttttga ggttgcgcca aagggctttg ggaccgacct gcccgttcat
     9961 ccattttacc aaaaacggtt tggcggtttt ccacaagtcc aaatcgggat cgagttggcg
    10021 gcccaagcct tcgatgttga gcagcgtttt ttgcagcaat accagctgcg gctggatttc
    10081 gacattgaag cggcggctga cttcaaacag gcgcatcagt accaagccga aggaaatctg
    10141 cgaaatcggt ttgttgaaca caggctcgca cacggcgcga accgccgctt ccaactcttc
    10201 cgcacgcgtg tcggcaggca cccagcccga ttcgacgtgg gcggtggcga cacgtcggta
    10261 atcgcggttg aaaaaggcga ggaaattgat ggcgagatag cgtttgtcgt aatcggtcag
    10321 cgtaccgacg atgccgaaat caagggcgat atagcggttg tcggcggcaa ccaaaatatt
    10381 gccggggtgc atatccgcgt ggaaaaagcc gtcgcggaaa acttgcgtga aaaagatttc
    10441 cacgccgtaa tcggcaagtt tgtgcaaatc gatgccgtct gctttgagtt tggcgatgtc
    10501 ggaaaccggc gtgccgtcca tccattcaat cgtcagcacg tcgcttgtgc agtagtcgta
    10561 aaacaccttg ggcacaatca gcatattgct gttttggaaa ttgcgcccga gttgtcctgc
    10621 attggcggct tcgcgcatca aatccaattc gtcgtgcagg tatttgtcga actccgccac
    10681 cacttcgcgc ggcttcagac gcttgccgtc tgaaaacaag cgttcgaccc aagccgcgcc
    10741 gaaacgcatc agcgacaagt cttgttcgat aaggggcaag aggttggggc gcaaaacttt
    10801 gaccgccact cgttcgcccg aatgcaggcg ggctttgtgt acttgggcga tggacgcgct
    10861 ggcgacgggc tcggtttcaa attccgcata cagcttttcg atgggctgac ccagcgattt
    10921 ttcgatttgt tcacgcgaaa gccgcgcgtc aaaaggcggc actttgtctt gcagctttgc
    10981 cagttcgacg gcgtaatcgt gcggaatcaa atcggggcgt gtggacaaaa cctgcccgaa
    11041 cttgatgaaa atcggcccca ggctttccaa ggcaaggcgc agacggacgg caggcggttc
    11101 gtttttcaat ttggacgact gcggcatcat tttcagcaga gcgcatatcc aaccgctacc
    11161 catcagcgaa acgcacagac ctgccagccg atagcggtaa aaagtcccga caatgaccgt
    11221 caggcgtttc aaccatttca tatataaaat cccgaataca gcatttatcg gacaatcaaa
    11281 aaatcttacc gagcagatag cccaaaaagg caatcagcaa cccgaaaacc agcggtttga
    11341 tattgcgggg cggcggctcg ggttgcgaag atgcggaaat atcatcttcc cgcccgccgt
    11401 gaagttcaag atattcgggc atttccccat ccgcgcctcc tccttcgccc tccccctcct
    11461 ccggcggcac gaccgtttcc aactccatat acgggtaata atatccaaac ccaaatagct
    11521 gaaataatca tgcaactctt cccgtttcgc ccaagtgcat ttttcctcaa ccagcacttg
    11581 cggtttggaa tcgagcgatt cgatcaatcc ttccgccttt tcttcgccga gattctcctt
    11641 aaggcactcc cggataagcc cgcgatccac ttggggcggg taggaaacat atacatcgta
    11701 caaatcgttt cggtaatctt tactcatctc aatcataagg gaacaaatac gcctgatacc
    11761 gttccgcccg ttcctttcgg gaaacccgat tcatcttcaa gcaatcggat tatatgtaaa
    11821 ttcatcccga taatatattt tccaaaagta aaaatgccgt ccgaacgttc agacggcata
    11881 acacaaacca gccggtcaca cggaaaacga cgatccgcaa ccgcaggttg tttccgcatt
    11941 cggattgcgg atgacgaact gcgaaccctg cagactttcc gtatagtcga tttccgcacc
    12001 gaccagatat tgatagctca tcggatcgac caaaaagacc aaaccgtttt tctcaatttc
    12061 aaaatcgtcg tcgtttttga tttcgtcaaa agtaaatccg tactggaaac ccgaacagcc
    12121 gccgccgttg acaaaaaccc gcaatttcaa atcgggattg ttttcttcgg caatcaaatc
    12181 ggcaactttg gcacagcagc tgtcagtaaa aataataggg ctttcgtccg acatggtgtt
    12241 attccttaat ataatatttt ctgcattctc tctcaacccc ccttcaaagg caagcagggc
    12301 ttcgcctttg aaaaaggaat tgtcccaata gataagtttg accgcctgca atttcaagcc
    12361 ccaaactcaa accgtcctgc cgaaccactc gacacgcccg ataatctcca catcatcggt
    12421 caagtcgttc aaattgattt caaaagcagg ataagcctcg ttggcggaaa tgacattgat
    12481 aatccc
//
LOCUS       DAXFHF010000060        11591 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXFHF010000060 DAXFHF010000000
VERSION     DAXFHF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.060"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..816
                     /locus_tag="ACFIRM_001660"
     CDS             <1..816
                     /locus_tag="ACFIRM_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3029246.1"
                     /translation="FSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADE
                     KAKLHRQAVEVEKLAKRFETQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYN
                     PEYSDGLLPDEGSIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRG
                     TLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLN
                     MALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            895..2127
                     /locus_tag="ACFIRM_001661"
     CDS             895..2127
                     /locus_tag="ACFIRM_001661"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC3029247.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCTELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKTFRGA"
     gene            2546..2854
                     /locus_tag="ACFIRM_001662"
     CDS             2546..2854
                     /locus_tag="ACFIRM_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029248.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            2938..3969
                     /gene="ruvB"
                     /locus_tag="ACFIRM_001663"
     CDS             2938..3969
                     /gene="ruvB"
                     /locus_tag="ACFIRM_001663"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC3029249.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMDEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAISESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(4214..4897)
                     /gene="rpe"
                     /locus_tag="ACFIRM_001664"
     CDS             complement(4214..4897)
                     /gene="rpe"
                     /locus_tag="ACFIRM_001664"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC3029250.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            5043..5477
                     /locus_tag="ACFIRM_001665"
     CDS             5043..5477
                     /locus_tag="ACFIRM_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC3029251.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(5490..6353)
                     /locus_tag="ACFIRM_001666"
     CDS             complement(5490..6353)
                     /locus_tag="ACFIRM_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3029252.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLALLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(6361..6978)
                     /locus_tag="ACFIRM_001667"
     CDS             complement(6361..6978)
                     /locus_tag="ACFIRM_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC3029253.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(7141..7755)
                     /locus_tag="ACFIRM_001668"
     CDS             complement(7141..7755)
                     /locus_tag="ACFIRM_001668"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC3029254.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            7962..9731
                     /locus_tag="ACFIRM_001669"
     CDS             7962..9731
                     /locus_tag="ACFIRM_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC3029255.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            9728..10384
                     /locus_tag="ACFIRM_001670"
     CDS             9728..10384
                     /locus_tag="ACFIRM_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC3029256.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(10585..11591)
                     /locus_tag="ACFIRM_001671"
                     /pseudo
     CDS             complement(10585..11591)
                     /locus_tag="ACFIRM_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2796 a         3377 c         2814 g         2604 t
ORIGIN      
        1 ttttccgcca ataaatccaa agcccgtcag gcaaccagcc gtctgaaaca ggccgacaaa
       61 atcaaatcgg aaatggtcga agtcaaacct tctacccgtc aaaacccgta tatccgtttt
      121 gaagccgatg aaaaagccaa gctgcaccgt caggctgtgg aagttgaaaa actggccaaa
      181 cgctttgaaa cccagttgtt tagaaacctc aacttcatcc ttgaagcagg ccaacgcctc
      241 gccatcatcg gcccgaacgg cgcaggtaaa tctaccttgc tgaaactctt ggccggcgcg
      301 tacaaccccg aatattcaga cggcctgttg ccggacgaag gcagcatcaa atgggcggaa
      361 aaagccagtg tcggctacta tccgcaagac catgaaaacg actttgacgt cgatatggac
      421 ttgagcgaat ggatgcgcca atggggtcgg gaaggcgacg acgaacaagt catccgaggc
      481 actttggggc gtttgctttt cggcagcaac gacgtggtaa aaaaagtgaa agttctctcc
      541 ggcggcgaaa aaggccgtat gctttacggc aaactgttgc tgttgaaacc caatgtcttg
      601 gtcatggacg aaccgaccaa ccatatggac atggaaagca tcgaatcgct gaacatggca
      661 ctggaaaaat acaacggcac gctgattttc gtctcccacg accgtcagtt cgtttcctcc
      721 ctggcaaccc aaatcatcga actggacggc aaaggcggat atgaacacta cttgggcgat
      781 tacgaaagct atctcgagaa aaaaggcgta gcataagcgc acccctccgg ttgaaacaat
      841 gccgtctgaa gccgcttcag acggcattgt tgataacttt aaaataggaa gcatatgcag
      901 acttacctcg tcggcggcgc cgtccgcgat tatcttttgg gcctgcccgt caaagaccgc
      961 gattgggtgg tcgtcggcgc agacgcgcaa accatgttgg cgcaaggttt ccagccggtc
     1021 ggcaaagatt ttcctgtatt cctccatccc aaaacgcacg aagaatacgc cctagcccgc
     1081 accgaacgca aaaccgccaa gggttacgcc ggtttcagtt tccacgccga caaagacgtt
     1141 acgctggaac aggacttgat gcgccgcgac ctgaccatca acgcgatggc gcaagatgcg
     1201 gacggcaaga ttatcgaccc tttcggcggg caacgggatt tggcggcagg cattttgcgc
     1261 cacgtctcgc ccgccttcgc cgaagatccc gtccgcatcc ttcgtgccgc ccgctttgcc
     1321 gcgcgttacg gctttgaaat cgccgaagaa accataaagc tgatgcggca gatggtggaa
     1381 aacggcgaag cggacgcatt ggttgccgaa cgcgtctggc aggagttggc gaaaggtttg
     1441 atggaaaaaa atccgcgcaa aatgattgaa atgttgcgcg aatgcggcgc actccaagtc
     1501 ttgctgcccg aagtcgacgc cctcttcggc gtgccgcaac gcgccgacta tcatcccgaa
     1561 atcgacagcg gcatccatac cctgatgacg ctgcaacgcg ccgccgatat gggtttgagc
     1621 ctgcccgaac gctatgccgc cctgctgcac gacttgggca aagccaaaac accgcccgac
     1681 atcctgccgc gccaccacgg acacgacatc aacggcgtag aacccgtgcg cgaagtcaat
     1741 caacggctgc gcgcgccgag gcagtgtacc gagcttgccg aattggtttg ccgttggcac
     1801 attattttcc accaagtcgg acagcttaaa agccaaacca ttctgaacgt attgaaaaaa
     1861 accgatgcct tccgccgtcc cgagcgtttt cagacggcat tgaacgtctg cattgccgac
     1921 acgcaaggcc gtctgaaccg cgaacacacg ccctacccgc aacgcgcgca ctggctcgcc
     1981 ctgctcgaag ccgccaatca ggcggattcg ggcaaaatcg ccgccgaatg ccgcgcgcag
     2041 ggaaaagcgc actttatcgc cgaacaaatc gaccgggcgc ggctggcgca aatcgccccg
     2101 ctgcaaaaaa cgtttcgggg cgcgtaggaa aaagaaaacg ccaaacgcgg catcttttga
     2161 ggttcgcccg cattgccgaa acagcgtcgg agaaagggat ggatgacatc ggtttattcg
     2221 gctgcctttt aatattccct tgatgcaggc ctgattagtc ggacaaaaat accttttgtt
     2281 cagcggaacc gatttctatc cggacggaaa cagccggctt tccccttgca aacggatgga
     2341 atcaagcggg tattcaaaca cagccgttta tttttattcc gccgccaagt tatgggatag
     2401 ggtttgcgtg tttcgggcag gcaaaccgcg caaccttaga cgcagtccga agttttgaag
     2461 gcggcatccg ggccgggttt tgaaacagcc gccgccatat ccgctataat ccatccttca
     2521 gccattccgc ccccgacata aaatcatgac cctgaaaacc gatttattgc ctaaaatcaa
     2581 caacgaagat tatcaacgcc tcatcctcaa acatagcgcg gaattcagcg gcggagaaac
     2641 ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg cacaggcatt
     2701 ggcgcaggcg gtgatgcagc aggtgcgctt cgaccccaat gcctaccaca tcgacagcga
     2761 cgacgaagac accacaggca tctgccccca ctgcatcaac ccgcctatgc cgcccctgcg
     2821 cgactatctc gtttggcgcg aaacacgcgg ataaaacgct tttgaccgtt atcttttcaa
     2881 tgccgtctga aacgcgccgc cgaccgttcg gacggcatac ccgacaaagg gaacactatg
     2941 ctgcaaaccg acaacctgac cgccgcgcaa ccgcaacgca tcgttgccgc ccaaaccgcc
     3001 tccgcacagg aagaactgct cgaacgcgcc ctccgcccca aaacgctgga cgactacatc
     3061 gggcagcata aagccaaaga gcagctcgcc atcttcatcc aagccgccaa aaaacgcggc
     3121 gaagcactcg accacgtcct gcttttcggc ccgcccggac tgggcaaaac cacccttgcc
     3181 cacatcatcg ccaaagagtt gggcgtaaat ttgcgccaaa ccagcggccc cgtcctcgaa
     3241 cgcgccggcg acctcgccgc ccttttgacc aaccttgatc cgcacgacgt attgtttatc
     3301 gacgaaatcc accggctaag ccccgtcgtc gaggaaatcc tctatcccgc gctcgaagac
     3361 taccggctcg acattatgat aggcgaagga cccgccgcac gttccgtcaa aatcgacctg
     3421 ccgcccttca ccctcgtcgg cgcgaccacc cgcgccggta tgctgaccaa cccgttgcgc
     3481 gaccgcttcg gcatcgtctc ccgcctcgag ttttacgaaa accgcgacct taccaccatc
     3541 gtcagccgtt cggcacaact gttgcagctc gatatggacg aagaaggcgc gatggaagtc
     3601 gccaaacgca gccgcggcac gccccgcatc gccaaccgcc tgttgcgccg cgtgcgcgat
     3661 ttcgccgacg tgaaaaacaa cggcgtaatc gatgccgccg tcgccgatgc cgctttaagt
     3721 atgctggacg tggacgcgca ggggctggac gtgatggaca ggaaattcct cgaagccgtt
     3781 ttgcacaaat tcggcggcgg ccccgtcggt ttggacaacg ttgccgccgc catcagcgaa
     3841 tctacggaca ccatcgaaga cgttatcgaa ccctacctca tccaacaagg ctttttgcaa
     3901 cgcaccccgc gcggcaggat ggcgaccgaa cgcgcctacc tgcatttcgg gctgcccgtc
     3961 gaaaaataaa ccatgccgtc tgaaaccgaa ccgggtttca gacggcattg ttatcagacg
     4021 tttgcaccgg caccggtttc atcacttaac agaaaccttt tgtccggcag gacagattct
     4081 ccccgatgaa aataactgtt tccctggcaa acggttgaaa cccgtctggc tgccggttga
     4141 aaaataacgc aatgctgtct gaaacagagc taattttcag acggcatttc tatttcaatc
     4201 attggctcaa ggttcagcct gccgcttttt ccagttccgc cctcatcgca gcgattaccg
     4261 ccttgtaatc cggtttgccg aaaatcgccg aaccggcaac aaaggtatcc gcaccggctc
     4321 gggcaacggc ggcaatattg tcagttttga tgccgccgtc cacttcaatg gcaatacgtc
     4381 tgccgctttt cccttcatag cggtcaagca tggcgcgaac ctgtctgatt ttttccaaag
     4441 tgtgcggaat gaagctctga ccgccgaacc cggggttgac cgacatcagc aaaaccatat
     4501 ccagcctgtc caacacgttt tccaacacat atacgggcgt tgccggattc aacaccagcc
     4561 ccgcctgaca gcccatgtcc cgaatcaggc tcaagctgcg gtcgatatgg cggctcgctt
     4621 cgggatggaa agtgataatc gatgctcctg ctttggcaaa cgattgaatt aaatcgtcaa
     4681 cgggttcgac catcagatgc acatcgatcg gcacgcttgc ataaggcttc aacgccgcgc
     4741 agaccatagg tccgaaggtc aggttcggca cataatggtt gtccatcacg tcaaaatgga
     4801 tcagatccgc acccgccgca atgacatttg ccacctcttc tccgaggcgg gcaaagtctg
     4861 ccgacaaaat actgggtgcg atacggtagg cggtcatatt ttttccttca atgcctttat
     4921 gggcggactt aacgcgggaa agcctcgaaa cagcattatt aacaaaagtt aactgccata
     4981 ataccatctt tcacacggca atcaagtata ttgtccgaaa tatccggatt aaaggaaaag
     5041 ccgtgccgct gcctgctccc tgccgttttg ccaaacctgc cgcctctttt ttaagtatgg
     5101 ctttgctttc ctgccagctt tcccacgccg ccacggctta tatccccccg aacgattttc
     5161 aaccgaactg cgacatacgc cggctcgggc tgacacaggg tcagcacaat gagctgcgta
     5221 aaatccgcgc cgccttcaaa atggcgggcg acagggcgcg tttgaaggtt atgcattccg
     5281 aacacagccg ccgccgctct gtcgtcgaaa tcatttcttc ggatgttttt aatcggaacg
     5341 aggcgcgcga ttatgtcgaa agccgctacc actccagcat ggattttgcg gtggacgaat
     5401 tggaaatcca acaccgcttc ttccatattc tcacaccgca acagcagcaa atgtggcttt
     5461 cttcctgcct caaataatcc ccgaaacact cacggcgcac gttgctccgg cagcctgccc
     5521 gcccagtcgc aggcaaactg ccaagcggaa cgtcccgagc ggttgccccg catctgcgcc
     5581 cactgcaacg ccgccgtccg ggcggcttcg tcatatggca catcaaaatc ttccaaccag
     5641 cttcgcactg ccgccagata atcgttttga tcgaacggat aaaaactgag ccacaatccg
     5701 aatcggtcgg acaaagatac tttttcttcc acggcctctt tttgatggat ttctccgcgc
     5761 acgcctgtcg tacctgcatt ttcatccaaa tattcgggca tcagatgccg tctgttggaa
     5821 gtcgcgtaca ccatcacgtt ggcgcaacgt tgggacagac cgccatccaa tgccgttttc
     5881 aatgccttat aggtttcgtc gccgctttca aatgacaaat cgtcgcaaaa cacaatgaat
     5941 ttttccgggc gttccttcaa aagtgccaac agatacggca ggccgatcaa atcgctttta
     6001 tcgacttcga tcaggcgcaa tcccttatcc gcatattcgt gcagcagggc tttgaccagc
     6061 gaggatttgc ctgttccccg cgcgccgctc atcaatacgt tattcgcagg tctgccggca
     6121 ataaattgtt cggtattacg caccagcaat tcggtctgcc tgccgactcc ggcaagccgc
     6181 gacaaaggga aaacgtgcgg atcgggcaag tgttccaaaa aacctttttt gcccgcactc
     6241 tgccagcgga aggcaagcgc gttccaatcc gtatgcccgg gttcgggcgg aagcacggca
     6301 tccaaacgcc gcaaaacggc ataggcttta tcgaggaatt cgttcaattc catctctacc
     6361 tcactttgca tatctttgcg ccatcagccg ttcgacggtg tcgacgattg cctgcgtatt
     6421 cggatcgatt tcgatgttga tcctgtctcc ggcctttctg ctgccgaaca gcgtccgttc
     6481 caaagtttcg ggaatcagat ggacattgaa acggctgtct tcgactttgc ctatggtcag
     6541 gctgcaaccg tccaagccga cgaacccttt ggtcaggata tagggtttga gttcgtgcgg
     6601 gagcgcaaac caaaccgtgc ggttgaaccc gtcccgttcg atttcgacaa tgggcacggt
     6661 tgccataatg tgtccgctca tgacgtgtcc gccgatttcg tcgccgaaac gtgccgcccg
     6721 ttcgatgttg acgcaatcgc cttccttcag cagccccaaa ttggtttttg ccaaagtttc
     6781 tgccattaaa tcgaagctga cgcggtttcc ttcgatttca gtaatcgtca ggcagcagcc
     6841 gttattggcg accgatgcgc cgtgttgcag gttttctgcc gcctcttgcg gaagctcgac
     6901 gacataagtc tgaaacgcct ccgacgggcg gtggattgcc gtcagttttc ccaatccttg
     6961 aacaatgcct gtaaacataa tcctgtttcc ctgtgtcggt aaaaatggtg caaattgtag
     7021 catttccccg cgaaaaatgc cgtctgaaac tccttcagac ggcattgtgc ctccggttcg
     7081 ggcaaaaacc gcccggtacg ccttgacctt tcctttccgc gccggtcggc ggtcttgcac
     7141 ttatccctcc tgcaaatcgg tttgcgtgtt caagtcggca aaatgcccgt caaactcgaa
     7201 tctgaccggc cgcgcccttt gctgctgcaa ccagcttctt aatgttttca tacccgaaaa
     7261 cagataagga atcgcgcttt gcagaatttg cggacggata tacataatgt tgtagtgcat
     7321 cgttaccggc gtttccacat aaaacgcatt gcacaacggt gtgcgtttgg agacgctttc
     7381 aaagcgcgcg accaaatcgt ccggcagata cggcatatcg caaggtacaa tcagcagcca
     7441 atcggccgcc gccaactgca aatcgttggc tgcggtacac aatgccgaaa gcgggccgaa
     7501 atgctgccac tgccgggcat ccggaaaaac atgcgggctg cggcgggcgt attcttccaa
     7561 attccggttg gcactgatga cgatatggct gacctgcggc ctgattttac cgataacgcg
     7621 gtcaatcagc ggtctgcctt ccaacagtgc cagccctttg tcttccccgc ccatacggtc
     7681 ggccaggcct cctgccaaaa tcaatgcgaa cgtttttaat gcgggcgggc ttttgggaaa
     7741 gtttgaggtt ttcatagttt cagtctgccg tttccgatat tttagaagcg cgtttgaaaa
     7801 cggcgtcgta taaattcatg ttatttgaat tttatcataa cgtgatttaa cactgaaagc
     7861 attgaggctt aagttttcct taacctgtcc tttctgagca gttgtttcta attccaaaga
     7921 atgatattgt ttgcaccgtt taaaacgact ttcctccgaa tatgatactg ccaacccgat
     7981 tttcagacgg catccccctt tccctgcgcc tgaaactcct gaccggcttg tgggtcggtt
     8041 tggcggcact gtctgtcgtt ttaacgctgc tgctttcttt ccgcctggaa aatgccgcct
     8101 cggtcatcga agaggcgggc aatctgaaga tgcaggcata ccggcttgcc tatatggcgg
     8161 gcgaaggctc gccccgcgca caaatcgaca atcagattgc cgaatttgaa aaaagtttaa
     8221 aacgcatttc ccaaagcgat gccatccatc cgctgattcc ctccgacaat ccccttgcct
     8281 acgatttgat acagtccatg ctgattatcg actggcaggc caacatcctc cccccgttgc
     8341 aggcataccg ccgccccaca caaatcgagc tttaccgctt tgccggcaat atcgaactgt
     8401 ttttgcaggc gttggaaaat gccggcgaaa aaaacacttg gtggctcagg cggtttcaat
     8461 gggtcattat gctgatgacg ctggtgtcgt ccgtgctgat gcttttttgg caccagattt
     8521 gggttatccg tccgctgcag gcgttaaggg aaggtgcgga acgcatcggt cagcggcatt
     8581 tcgacatccc tgttcccgaa gacggtacgc ccgaattcaa acaggtcggg cggtgtttca
     8641 accaaatggc gctcaggctg aagactttat acgatgattt ggaaggacag gttgccgaac
     8701 aaacccacaa ccttgaaaag caaaaccgca acctgaccct gctgtaccgg accacgcgcg
     8761 acctgcatca atcctatacg ccgcgacagg cggcggaaga atttttgaac cacatcctgc
     8821 ccgccgtcgg cgcgcaatcc ggcaacatct gcctggaaaa cggttccgac acagacattt
     8881 ccgtccatac ggccgaacac ggcaaaaagc cccctttgga aaaataccat gacgaaacct
     8941 tccccatcga atatcaaaac gagaaactgg gcatgctttc gctcggcttt tcagacggca
     9001 cttccctcac cggagacgac cgcacgctgc ttcaaaccct gattcggcaa ttgggcgtat
     9061 cgctcgccgg ggcgaaacag gaggaagaaa aacgtctgct tgccgtattg caggaacgca
     9121 gcctgattgc acaggggctg cacgacagca tcgcgcaggc attaaccttc ctgaacctgc
     9181 aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc gcagaaaaca
     9241 tcggctttat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa ctgctgctca
     9301 atttccgtac caaaatcagc aataaagaat ttcccgaagc tgttgccgac ctgttcgcgc
     9361 gcttcacgca acaaaccggg ataacggtcg aaaccgtttg ggaaaacggt tcgttcctgc
     9421 ctacacagga cgagcagctc caaatgattt tcatcctgca agaaagcctg tccaacatcc
     9481 gcaaacacgc ccgcgccacc catgtaaaat tcaccctttc cgaatacggc ggacgcttta
     9541 ccatgaccat ccaagacaac ggacaaggtt tcgacacgga gaaaatagga gaacctacgg
     9601 gcagccatgt cggactgcac atcatgcagg agcgtgccaa acgcatccgt gccgttttag
     9661 aaatccgttc ccaagcacaa cagggaacca ccgtctcatt gacgggcgca cccaaagaaa
     9721 gcctaccatg accatcaaaa tcatcctgat agacgaccac accctcttcc gcagcggcat
     9781 caaagccctt ttgtcgcgcc aacacggttt tgaagtcatc ggcgaagccg cagacggact
     9841 ctcgggcatc aaaatgatca gtcggctgca acccgatgtc gtcctgctcg atttggatat
     9901 gccaggcatg aacgggcgcg aggccttgtc ccaaatcatc agcatcaatc cgcagcaggc
     9961 agtcattatg ctgaccgttt ccgaagacag cgacgacctt accgaatgta tgcgcatcgg
    10021 cgcgcgcggc tacctgctga aaaacatcaa cgccgatttt cttctcgaaa gcatacgcaa
    10081 agccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg tcaaaagcct
    10141 gatttccccc caacctgccc aaaggacgca ggcactctcc tcacttaccc ctcgtgaact
    10201 ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc gccacctcga
    10261 tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac tcaacctcag
    10321 cagccgggtg caggccgccg tttacgccat ccggcacaac gtcccccaac ctgtgccgga
    10381 atagacgttc agacggcatc gggtcaggaa aaaagcaatg cgcccctttg acggggtgca
    10441 atatataagg gacggcacac cggatgccgt ccctttaagt acgccattac cgccgtaacc
    10501 cggacggaat ggcttaggca gtttatttcc aagaatattt cacacccacg gtgccttggt
    10561 agcctttatg tctgttgctt ccgcatcctg ccgataattg ccttgcctgt cccgagaaag
    10621 ggtttgattt tcgccacggc gttggtggag cgtatccagc atcgcatagc ctgattccaa
    10681 gttttgacgt ggaacgagtg cgtaggccgg tgcgcttggc gtgatgatgg ttttatcttg
    10741 attcggcgtg gttaaagtcc agaagtattc tgtcgcgccc gcctcatctt tgtgtgaaac
    10801 caattgcaat tcccccgccc ctgtggtttt ggcagttccg ctaaaagtat cgcgcattgt
    10861 gtaggtatgt ttagcgtctt cgatatttaa tttaccggtt tccgccccct tgtcttgtcc
    10921 caaaacctta acaacggcgg cactgcgttt gtagcggtcg gaaagttcgc cgatcgagcc
    10981 gtcgataata ttttctttgc catctttacc cactgaaatg accgttgttt ttcctgaagc
    11041 atcgcctgtg atttccagta aatcagattg agaatttcct ccttgatcat cacccggcga
    11101 gttccattct gtattgactt ttaacacacc gtctttaccg acataatttc cttcaatggt
    11161 gagtttgtct gcataggatt gattggcaag cgtaatgatg ccgccgttgg tgaatgttcc
    11221 tttttcaaat acagaagttg tacccgataa agaaatacct gtattagata gggttagcgt
    11281 gccgcctgat aataatgtag tcccttcggg ggttgttaat gatgtgtctt tttttaggtg
    11341 aaaagtagct tttgttagtt ttatggttcc aatatcttct gatttgaatt ccattgcgcc
    11401 atttgtaatt tttagcacag cattggtaat agggaggggg aagcgcattg taaatgattt
    11461 ttctgttcca tctggattga ttaatgtacc agatgcgcca aagatctctc cttcagggat
    11521 agaaacagtt ccatcagaaa ttgatggttt atttgcgctt ttatcactgt gtttatgaat
    11581 taatgtaatt g
//
LOCUS       DAXFHF010000061        11526 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXFHF010000061 DAXFHF010000000
VERSION     DAXFHF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.061"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(64..1143)
                     /gene="apbC"
                     /locus_tag="ACFIRM_001672"
     CDS             complement(64..1143)
                     /gene="apbC"
                     /locus_tag="ACFIRM_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC3029257.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLGIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1361..1436
                     /locus_tag="ACFIRM_001673"
     tRNA            1361..1436
                     /locus_tag="ACFIRM_001673"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1394..1396,aa:Lys,seq:ttt)
     gene            1516..2025
                     /locus_tag="ACFIRM_001674"
     CDS             1516..2025
                     /locus_tag="ACFIRM_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC3029258.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2366..2806
                     /gene="nadR"
                     /locus_tag="ACFIRM_001675"
     CDS             2366..2806
                     /gene="nadR"
                     /locus_tag="ACFIRM_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC3029259.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            2921..3421
                     /gene="hpaC"
                     /locus_tag="ACFIRM_001676"
     CDS             2921..3421
                     /gene="hpaC"
                     /locus_tag="ACFIRM_001676"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC3029260.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3437..4132
                     /gene="murU"
                     /locus_tag="ACFIRM_001677"
     CDS             3437..4132
                     /gene="murU"
                     /locus_tag="ACFIRM_001677"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC3029261.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4166..4578)
                     /locus_tag="ACFIRM_001678"
                     /pseudo
     CDS             complement(4166..4578)
                     /locus_tag="ACFIRM_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            4771..6447
                     /locus_tag="ACFIRM_001679"
     CDS             4771..6447
                     /locus_tag="ACFIRM_001679"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC3029262.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6561..7652
                     /gene="ychF"
                     /locus_tag="ACFIRM_001680"
     CDS             6561..7652
                     /gene="ychF"
                     /locus_tag="ACFIRM_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC3029263.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7690..7929
                     /locus_tag="ACFIRM_001681"
     CDS             7690..7929
                     /locus_tag="ACFIRM_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029264.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            7926..9800
                     /locus_tag="ACFIRM_001682"
     CDS             7926..9800
                     /locus_tag="ACFIRM_001682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC3029265.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            9862..11157
                     /gene="tyrS"
                     /locus_tag="ACFIRM_001683"
     CDS             9862..11157
                     /gene="tyrS"
                     /locus_tag="ACFIRM_001683"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC3029266.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2553 a         3151 c         3215 g         2607 t
ORIGIN      
        1 atgaagcccg ccggcaggcg cggggcttgg gacggcattg ctgctgcggt ttcggacgcg
       61 gctttattcg acgacaattt tagggaaccg gctgctgaag tctttgcctt tgtcggcaat
      121 gcccagggcg atttggaatg cggcatcggt gtagattcgg gcgatggcgg ggtgttcgtc
      181 gaacagttgc gccggtgtgc cgccgtccat ggcttcgcgc acgggcaggc ttaggggaag
      241 ctgtccgagc agggggacgt tgaggcgggc cgccaaatct ttgccgccgt ccgtgccgaa
      301 cagtgcttcg ctatgcccgc agttggagca gatatggacg gacatgtttt ccaatacgcc
      361 caaaatcgga atgttgacct tgcggaacat atccacagcc ttgcgcgcgt ctatcagcgc
      421 gatgtcctgc ggcgtggtta cgatgacgga cccggttacg gggatgcgtt gggacagcgt
      481 gagttggatg tcgcccgtgc cggggggcag gtcgatgaaa agatagtcca cttcgtccca
      541 ctcgctttgg aacatcagct gctgcaaggc ttggctgacc atcggcccgc gccagacgac
      601 ggcttggccg gtatcgacga gaaagccgat ggacatgacc tgtatgccgt ctgaagattc
      661 gacgggaatg agtttttggt ttttctgatc gggtttgcgg tcgtgtacgc ccagcatcgt
      721 gggctggctc gggccgtaaa ggtcggcatc gagcacgccg acgcgcgcgc ccatgcgcgc
      781 cattgcggcg gcaaggttgg cagtggtggt tgatttgccc acgccgcctt ttcccgatgc
      841 gacggcgatg atgtttttca cgcctttgat ggtggcgacg ccgggccgga ctttgtgtgt
      901 gccgatttcg gtgtctatgc ccaagtggat gtgcgcgccg cctgtttcgg gcatcagggt
      961 ttcttgtacg gcatcggcga gggcggcggc gatgtgcgcg acagggaagc cgaaatgcag
     1021 ggcgatgtgg atgccgtctg aacgctgttc gaccgaacgg acggcctttt cgccgccgag
     1081 cgtgcgtgcc gtattcggaa cggcgacggt gtcgaggagg gtgcggatgt tttgtatatt
     1141 catgatgtgt gcctctgttc gcaagtcgtg tgaggggaag atagacggaa agtgtacatg
     1201 atttcgggcg gtttgctttt ccttgtgcga acttttacac ggtatgcggc ggattgtgaa
     1261 aatccgttgc caaaatatcc cgtgcaaaca gcagtctgca attaagcctg taaaatttgc
     1321 ttgacggcgg ggaacggggc tgtataatgc acggcttatc gggtcgttag ctcagccggt
     1381 agagcagcgg acttttaatc cgttggtcga gcgttcgaat cgctcacgac ccaccagata
     1441 accggaagcc aagtttcgag acttggcttt tttgtttgcc tgccggttgc gcgggcgtac
     1501 cgtttgcggg ggtgtatgaa aaagaaactg ctttcgggca tcaaatttgc cgttcggacg
     1561 gcattggtgt ttttgctggt gtcgctgttt ttggattggg tacgcaagcc cgacgaacct
     1621 gccggggcgg cagggcggcc tttgaccctg ctgtcggggc agcggctgac tttgggacag
     1681 tttagccggg ataggacggt gctggtgtat ttttggggaa gctggtgcgg cgtgtgccgt
     1741 tatcagtcgc cgataatcga tgatttggcg gcggacggcg tgccggtcgt cggcgtggcg
     1801 gtacgttccg gcagcgcgtc cgaagtggcg gcatatatgg caaaacgggg cttgggcttt
     1861 ccgactgtca gcgatgagga cgggggtttg gcaaggtctt ggcggattgc cgcaacgcct
     1921 gccgtcgttt tggtcaaaaa tgggaaaatg gtccgctata cgacgggaat cagcagttat
     1981 tggggcttgc gcgcacgaat ttttcaggcg gattttttcg gttaactttt gtttttgtca
     2041 aactttccgc tatgtagaga atcaaacggc atatgccttg cgcggcggat gcggcggctt
     2101 ttgttttttg ggaaagagcc ggcgcgcctg ccgatgtaat cccttgttgt gattgtggga
     2161 aaaatagatt aaaatataac tattaaaata ttttcagata ggattatcgg aattaaagtc
     2221 ttttataccc ggtcgtccga tgaggtttat agcgtattgt tgctatatgt tcgttttgtt
     2281 atataacggt tgcatcaaaa attacgccca caggctttcc cgacggtttg aaagtttgat
     2341 tttcgataac ttggagactt aaacaatgcc tacccaatca aaacatgcgt ctatcaatat
     2401 cggtctgata caggcaaggg aagccctgat gacccaattc aggcctattc tgaatcaggc
     2461 gaatattacc gatcagcaat ggcggattat ccgtcttttg gcggaaaacg gcacattgga
     2521 ttttcaggat ttggcgaatc aggcgtgcat tttgcgcccc agcctgaccg gtatcctgac
     2581 ccgccttgaa aaagcggggt tggtcgtccg cctgaaacct tccaacgacc aacggcgcgt
     2641 ttatctgaag ctgacttccg agggcgagaa gctgtatgag gaaatcggcg aagaagtgga
     2701 cgagcgttac gacgctatcg aggaagtgct gggccgcgag aaaatgctgc tgcttaaaga
     2761 cctgttggca gaacttgcca aaatcgagga tgcgttgaac tcgtaatacg ccgtaatgcg
     2821 cggaaacgtc cgaccgacgg ctttttgaat cagaactgct gcacatgggg gatgccttgt
     2881 gtgcagcatt cttatatagg ggacggttta aaggggaaaa atggcggatt tgcagaaaac
     2941 ttttcaaact tcgttccgtg atgcgatggc atcctgtgcg gcaggcgttc atgtcatcac
     3001 gacagacggt gcggcagggc gttacggcat tacaatgacg gcggttgcgc cggttaccga
     3061 cgagccgccg accgtgatgc tgtgcataaa ccggagtgcg cgaatcattc cgatcctgtc
     3121 ggaaaacggc agcctctgca tcaatatgct ggcggacgaa catcaggatg ttgccgaaca
     3181 ttttgccggg ctgaccggcc tgtcgcccga agagcggttt gcctaccata tttggcatcg
     3241 cggcaaaacg ggacaacttg aaatagaagg cgcgttggcg cacctgcacg ggcatattgt
     3301 cggcaaacat gaaatcggca cgcattttgt gttttacgtc aggctcgacg aaatcaaaaa
     3361 ctgcgggtgc aaacgccccg cgctgctgta tttcagacgg cagtttaggc ctttagactg
     3421 atattcggac agatatatga aagcgatgat actggcggca ggacgcggcg agcgtatgcg
     3481 ccctttgacc gacaccactc cgaagccgct gctcgatgtg gcgggtaagc ctctaatcgg
     3541 ttggcacttg tgccgtctga agcaggcggg gtttaccgaa atcgtcatca accacgcttg
     3601 gctgggtcgg cagatagaaa atgctttggg cgacggctcg gcttatggcg tgaacatcgc
     3661 ctattcgccc gaacccgcag gcggtttgga aacggcaggc ggcatcgcgc aggcattgcc
     3721 gctgttgggt gggcagccgt ttttggtggc caacggcgac gtgctgaccg acatcgattt
     3781 taccgccgcg tttcagacgg catcgtccct gcccgggcac atttccgcac atttgtggct
     3841 ggtgggaaat cccccgcaca accccgacgg cgatttttcc ctgctgcccg acggcagcgt
     3901 gcggccggaa gtatccggcg gcaacggact gacattcagc ggcgtgggta tttaccgtcc
     3961 tgaaatgttt gacggaatcg aagcgggcag tgtggcaaaa ctcgcgcccg tattgctgaa
     4021 cgaaatgcgg caaaaccgcg tgagcggtca gaagcatacg ggcctgtggc tggatgtcgg
     4081 cacggtatgc cgtctgaagg aagcgcaagc ccttgcagcg gcttggaagt gaaaactcgg
     4141 tttcaggcgg tatggcggat tcggtttaac gtttcagtgc caacgtcaac acgccgccgt
     4201 aaccagcccc aagtctatcc attcctgcgt gttcgggcgt tcgtctaaga aaatcaccgc
     4261 catcagcgcg accaagacca ggctgaattt gtcgacgggc gcgacttgcg gggcgttgcc
     4321 cagttgcagg gctttgaagt aggcgagcca agatgcgccg gtggcgagtc cggacaaaac
     4381 cagaaacgtc cggttgcgcc ccgtgaagcc gtttacgccc tgccatttgc cggtgtaggt
     4441 caaaaacaat accaaggcgg cgaggatgac caaggtgcgg ataaaggcgg cgaaatccga
     4501 atctatgccc tgcaagccca ttttggcgaa aacggcggtc aatgaggcga agcctgccga
     4561 tgccaatgcc caaaacagct atgcgttgct gcccatgttt tctccttgga ttgtgaacaa
     4621 tatgaacggt atttttgttg ctgcgtcaaa aatttcactg cgggtttggt gcggataacg
     4681 ttataatatg cctgatatta ttttcaatcc acctgtttgt cgcctgatgc tttcagacgg
     4741 catgtccctc ctcatttcta aaggaaaatc atgagcttca aaaccgatgc cgaaaccgcc
     4801 caatcctcca ccatgcgccc gattggcgaa attgccgcca agctgggttt gaacgttgac
     4861 aacattgagc cttacggtca ttacaaagcc aaaatcaatc ctgccgaagc gttcaagctg
     4921 ccgcaaaaac aaggcaggct gattttggtt accgccatca acccgactcc ggcgggcgaa
     4981 ggcaaaacca ccgtaaccat cggtttggcg gacgcattgc gccatatcgg caaagactct
     5041 gtgattgctt tgcgcgagcc ttctttgggt ccggtgttcg gcgtgaaagg cggcgcggca
     5101 ggcggcggct acgcgcaagt tttgccgatg gaagacatca acctgcactt caccggcgac
     5161 ttccacgcca tcggtgcggc gaataacctc ctcgccgcca tgctcgacaa ccatatctac
     5221 caaggtaacg agttgaacat cgaccccaaa cgcgtgctgt ggcggcgcgt ggtcgatatg
     5281 aacgaccgcc agttgcgcaa catcatcgac ggtatgggca agcctgttga cggcgtgatg
     5341 cgtcccgacg gcttcgacat caccgtcgcc tccgaagtga tggcggtatt ctgccttgcc
     5401 aaagacatca gcgatttgaa agagcgtttt ggcaatattc tcgtcgccta cgccaaagac
     5461 ggcagccccg tttacgccaa agatttgaag gcacacggcg cgatggcggc attgctaaaa
     5521 gatgcgatta agcccaattt ggtgcaaacc atcgaaggca ctccggcctt tgtacacggc
     5581 ggcccgttcg ccaacatcgc ccacggctgc aactccgtta ccgcaacccg tctggcgaaa
     5641 caccttgccg attacgccgt aaccgaagca ggcttcggcg cggacttggg tgcggaaaaa
     5701 ttctgcgaca tcaaatgccg ccttgccggt ttgaaacctg atgcggcagt cgtcgtggcg
     5761 actgtccgcg ccctgaaata caacggcggc gtggaacgcg ccaaccttgg tgaagaaaac
     5821 ctcgaagcct tggcaaaagg tttgcccaac ctgttgaaac acatttccaa cctgaaaaac
     5881 gtattcggac tgcccgtcgt cgttgcgctc aaccgcttcg tgtccgactc cgatgccgag
     5941 ttggcgatga ttgaaaaagc ctgtgccgaa cacggcgttg aagtttccct gaccgaagtg
     6001 tggggcaaag gcggcgcggg cggcgcggat ttggcgcgca aagtcgtcaa tgccatcgac
     6061 aaccaaccta ataacttcgg tttcgcctac gatgtcgagt tgggcatcaa agacaaaatc
     6121 cgtgcgattg cccaaaaagt gtacggcgcg gaagatgtcg atttcagcgc ggaagcgtct
     6181 gccgaaatcg cctcgctgga aaaactgggc ttggacaaaa tgccgatctg catggcgaaa
     6241 acccaatatt cattgagcga caacgccaaa ctcttgggct gccccgaagg cttccgcatc
     6301 gccgtacgcg gtatcactgt ttccgccggc gcgggcttca tcgttgcgtt gtgcggcaat
     6361 atgatgaaaa tgccgggcct gccgaaagtt ccggctgccg agaaaatcga tgtggacgaa
     6421 cacggcgtga ttcacggctt gttctgaacg gtttttgaaa ccggatgccg tctgaagccg
     6481 tttcagacgg cattttttcg gaacgcgggc ggcggtatgc tataattctc cgttaaattt
     6541 ctctattttc aggaaaaacc atgagtttga aatgcggcat cgtcggtttg cccaacgtcg
     6601 gcaaatccac cctttttaac gcgctgaccc aatcgggcat cgaagcggca aactatccct
     6661 tctgcaccat cgaacccaac gtcggcatcg tcgaagtgcc cgacccgcgt atggcggagc
     6721 tggcgaaaat cgtcaatccg caaaaaatgc agcccgccat cgtcgagttt gtcgatattg
     6781 ccggcttggt tgcgggcgcg agcaaaggcg aaggcttggg caaccggttc cttgccaaca
     6841 tccgtgaaac cgatgccatc gtcaacgtcg tgcgctgctt tgacgacgac aacatcgtcc
     6901 acgtttccgg caaagtcgat ccgattgccg acatcgaaac catcggcacc gaattggcgc
     6961 ttgccgactt ggcaagtgtg gaaaaagcca tcgtccgcga agaaaaacgc gcccgatcag
     7021 gcgacaaaga cgcgcaaaaa ctagtcgatt tgtgcaaaaa actgctgccg catctggacg
     7081 aaggcaaacc cgtgcgttcc ttcggtttgg acgcggaaga acgcgcgctg ctgaagccgc
     7141 tgttcctgct gaccgccaaa cctgcgatgt atgtgggaaa cgttgccgaa gacggttttg
     7201 aaaacaaccc gcacctcgac cgcctgaaag aattggcggc aaaagaaaac gcccccgtcg
     7261 tcgccgtctg cgccgcgatg gagagcgaaa tcgccgaatt ggaagacggt gaaaaagccg
     7321 aatttctcgc cgaaatgggc ttggaagaac cgggcttgaa ccgcctcatc cgcgcgggtt
     7381 acgacctttt gggactgcaa acctacttca ccgccggcgt gaaagaagtc cgcgcgtgga
     7441 cgatacacaa aggcgacacc gcgccgcaag ccgccggcgt gatccatacg gattttgaac
     7501 gcggcttcat ccgcgcccaa gtgattgcct acgatgattt tgtctcgctc ggcggcgaag
     7561 ccaaagccaa agaagccggc aaaatgcgcg tggaaggcaa ggaatacgtc gtgcaggacg
     7621 gcgacgtgat gcactttttg tttaacgtgt aacccaaatg cggcaggttt tcagacggct
     7681 ttgccggaaa tgccgtctga agccggtttt ggtggttttc gacgttccca taccgccgga
     7741 atgcagccgc atcaaaataa aatcccgccc gcatttccga tttgccctcc ccgattcctg
     7801 caaaacaaac cgcctgccct gccgttacgg gaagccgtcc ggtattccga atatcccgaa
     7861 ccccgataca aaatgacctt tcagacggca tttgcacagc ccgccgcgtt tcaagtaaaa
     7921 acattatgag ccaagcctta ccctaccgcc cggacatcga cacattgcgc gccgccgccg
     7981 tcttgtccgt catcgtgttc catatcgaaa aggattggct gccgggcggg tttctcggtg
     8041 tcgatatatt ctttgtgatt tcaggctttt tgatgacggc gatcctcctt cgcgaaatgt
     8101 ccggggggcg tttcttcctc aagacatttt atatccgccg catcaaacgg attttgcccg
     8161 catttttcgc cgtattggcg gcaacgctgg caggcggctt ctttttattc accaaagatg
     8221 atttctttct tttgtggaaa tccgcgctga ccgccttggg tttcgcctcc aacctgtatt
     8281 ttgcaagggg gaaggattat ttcgatcccg cgcaggaaga aaagcccctg ctgcacatct
     8341 ggtctttgtc ggtcgaagaa caattttact ttgtctttcc gatattgctg ttgcttgtcg
     8401 cccgcaaaag cctgcgcgta cagttcggct tcctcgccgc attgtgcgcc ttaagccttg
     8461 ccgcttcctt tatgccttcc gcgctcgata aatattacct gccccacctg cgcgcctgcg
     8521 aaatgctggt cggatcgctg accgccgtgc ggatgcggta ccggcaacag cggaatcccg
     8581 ccgtcgggaa acggtatgcc gccgtcggcg cattgttttc cgcgtgcata ctgtccgcct
     8641 gcctgtttgc ctattcggaa caaaccgcct atttcccggg ccccgccgct ttgattccct
     8701 gtctggctgt tgccgcgctg atttatttca accattacga acacccgctt aaaaaatttt
     8761 tccaatggaa aatcaccgtt gccgccggtt tgatttccta ttcgctttat ctgtggcatt
     8821 ggccgatatt ggcctttatg cgctatatcg gcccggacaa cctgccgcct tattcgccgg
     8881 cggcagcgat cgtcctgacc ctggcgtttt ccctgatttc ttatcactgc atcgaaaagc
     8941 cgtttaaaaa atggaaaggc tcgttcgcac aatccgtttt atggatttat gccttgccta
     9001 tgctcgtttt gggcgcgggc tcgtttttcg cgatgaggct gccgtttatg gcgcaatacg
     9061 accgcttggg gctgacgcgt tccaacacct cctgccacaa caataccggc aaacaatgcc
     9121 tgtgggggga tacggaaaaa cagccggaac tgctggtttt gggcgactcc cacgccgacc
     9181 attacaaaac attcttcgat gccgtgggca aaaaagaaaa atggtccgcc actatggttt
     9241 ccgccgacgc ctgcgcctat gtggaaggct acgcgtcccg tgtgttccaa aactgggccg
     9301 cctgccgcgc cgtttaccgc tatgccgaag aacacctgcc ccggtatccg aaagtggttt
     9361 tggcgatgcg ctggggcagc cagatgcccg aaaacagccg ctcccttgcc tacgatgccg
     9421 gttttttcca aaaattcgac cgtatgctgc acaaactctc atccgaaaaa caagccgttt
     9481 acctgatggc ggacaacttg gcttcgtctt acaacgtcca gcgcgcctat atcttgtctt
     9541 cacgcatacc gggttgccgc caaacactgc gcccggacga cgaaagcacc ctgaaagcca
     9601 atgcccgcat cagggaattg gcagccaaat accccaacgt ctatattatt gatgccgccg
     9661 cctatatccc cgcagatttc caaatcggcg gattgccggt ttactcggac aaagaccaca
     9721 tcaaccctta cggcggcaca gaattggcga agcgtttttc cgaaaaacaa aggtttctcg
     9781 atacgcgcca taaccattga ttcgcttaaa tttgttacaa tcggcggttt gcaaaaccgc
     9841 taattttttt gaaagagacc gatgagcgtc atccaagacc tgcaatcgcg cggccttatc
     9901 gcgcaaacca ccgacatcga agccttagac gctttgctga acgaacaaaa aatcgccctt
     9961 tactgcggtt tcgacccgac agccgacagc ctgcacatcg gacacctgct gcccgtattg
    10021 gcattgcgcc gcttccaaca ggcggggcat acgccgattg cactggtggg cggcgcgacc
    10081 ggtatgatcg gcgaccccag cttcaaagcc gccgaacgca gcttgaattc cgccgaaacc
    10141 gttgccggct gggtaggaag catacgcagc caattaaccc ctttcttgag ctttgaaggc
    10201 ggaaacgccg ccattatggc gaacaatgcc gactggttcg gcagcatgaa ctgcctcgac
    10261 ttcctgcgcg acatcggcaa gcatttctcc gtcaacgcca tgctgaacaa agaatccgtc
    10321 aaacagcgca tcgaccgcga cggcgcaggc atttccttta ccgagttcgc ctattccctg
    10381 ctgcaaggct acgacttcgc cgagttgaac aaacgccacg gcgcggtttt ggaaatcggc
    10441 ggttccgacc agtggggcaa catcaccgcc ggtatcgacc tgacccgccg cctgaaccaa
    10501 aaacaagtgt tcggtctgac cctgcctttg gttaccaaat ccgacggtac caaattcggc
    10561 aaaaccgaag gcggcgcggt gtggctgaac gcgaaaaaaa cctcgccgta ccagttctac
    10621 cagttctggc tgaaagtcgc cgatgccgat gtgtataaat tcctgaaata ctttaccttc
    10681 ctgtccatcg aagaaatcgg tgtcatcgaa gccaaagaca aggcaagcgg cagcaagccc
    10741 gaagcgcaac gcatcctcgc cgaagaaatg acccgcctga ttcacggcga agaagccctt
    10801 gccgccgcgc aacgcatttc cgaaagcctg tttgccgaag accaaagccg gcttaccgaa
    10861 agcgacttcg agcagctcgc cctcgacggc ctgcctgcat ttgaagtttc agacggcatc
    10921 aacgccgtcg aagccttggt caaaaccggc ttggcagcgt ccaacaaaga agcgcgcggc
    10981 tttgtcaacg ccaaagcggt tctgctcaac ggcaaaccgg ctgaagccaa caaccccaac
    11041 cacgccgccg aacgccccga cgatgcctat ctgttgatag gcgaatacaa acgtttcggc
    11101 aaatacacca tcctccggcg cggcaaacgc aaccacgcgc ttttggtttg gaaataatcc
    11161 gattgccgca gaaatgccgt ctgaagcttt cagacggcat ttttatcaaa tgcaaaacac
    11221 cctgcgcctg ccgatatgtc gtcatttcca tgcaggcggg aattcaaact tgtccgcacg
    11281 gaaacttatc gggcaaaacg gtttcttcag ttctacgttc tagattcccg ctttcgtggg
    11341 aatgacggtt cagttgctac ggttactgtc aggtttcggt tatgttggaa tttcgggaaa
    11401 cttatgaatt gtcattcccg cgaaagtggg aatccaggac tcaaaatctc aagaaaccgt
    11461 tttgcccgat aagttcctgc actgacagat ctagattccc gcctgcgcgg gaatgacggg
    11521 tttcga
//
LOCUS       DAXFHF010000062        11394 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXFHF010000062 DAXFHF010000000
VERSION     DAXFHF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.062"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..107
                     /locus_tag="ACFIRM_001684"
     CDS             <1..107
                     /locus_tag="ACFIRM_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC3029267.1"
                     /translation="EEVNYERNKLRVSVQIFGRETPVELEFSQVEKIN"
     gene            208..642
                     /gene="rplK"
                     /locus_tag="ACFIRM_001685"
     CDS             208..642
                     /gene="rplK"
                     /locus_tag="ACFIRM_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC3029268.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            642..1337
                     /gene="rplA"
                     /locus_tag="ACFIRM_001686"
     CDS             642..1337
                     /gene="rplA"
                     /locus_tag="ACFIRM_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC3029269.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            1565..2065
                     /gene="rplJ"
                     /locus_tag="ACFIRM_001687"
     CDS             1565..2065
                     /gene="rplJ"
                     /locus_tag="ACFIRM_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC3029270.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            2123..2494
                     /gene="rplL"
                     /locus_tag="ACFIRM_001688"
     CDS             2123..2494
                     /gene="rplL"
                     /locus_tag="ACFIRM_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC3029271.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            2685..6863
                     /gene="rpoB"
                     /locus_tag="ACFIRM_001689"
     CDS             2685..6863
                     /gene="rpoB"
                     /locus_tag="ACFIRM_001689"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC3029272.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(6828..6968)
                     /locus_tag="ACFIRM_001690"
     CDS             complement(6828..6968)
                     /locus_tag="ACFIRM_001690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029273.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7016..11191
                     /gene="rpoC"
                     /locus_tag="ACFIRM_001691"
     CDS             7016..11191
                     /gene="rpoC"
                     /locus_tag="ACFIRM_001691"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC3029274.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            complement(11208..>11394)
                     /locus_tag="ACFIRM_001692"
     CDS             complement(11208..>11394)
                     /locus_tag="ACFIRM_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029275.1"
                     /translation="FAIFNTITFGFNSFYSMHNLTARRYGTLIGLISTRLKMTKTLVF
                     ALIGQRKTASSRLAVFY"
BASE COUNT         2983 a         2570 c         2966 g         2875 t
ORIGIN      
        1 ttgaggaggt caattatgaa cggaataagt tacgcgtgtc tgttcagata tttggtagag
       61 aaacacccgt tgagctggag ttcagccagg ttgagaagat taactgattt ttatacttga
      121 aaaaaaagca ataagaggat agaatcaaaa actaacttgg ggagcggaaa tggttccgcg
      181 tcttacccgt ttttaggagt ccgttaagtg gcaaagaaaa ttatcggcta tattaaactg
      241 caaattcctg caggcaaagc caatccatcc cccccagttg gtcctgcttt gggtcaacgt
      301 ggtttgaata tcatggaatt ctgtaaagca tttaatgctg caacccaagg tatggaatct
      361 ggtttgccaa ttccggttgt gattactgca tttgcagata aatcattcac atttgtgatg
      421 aaaactcctc cggcttctat tctgttgaaa aaagccgccg gtttgcaaaa aggtagttct
      481 aatcctctga caaacaaggt aggtaaattg acccgtgccc agttggaaga aattgctaaa
      541 actaaagagc ctgatttgac tgctgctgac ttggatgcgg ctgttcgtac tatagcaggt
      601 tctgctcgct caatgggctt ggacgtggag ggtgttgtat aatggctaaa gtatctaaac
      661 gcttgaaagt tcttcgctct tctgttgaag ctaacaaact gtacgcaatc gacgaagcaa
      721 ttgccttggt taaaaaagct gctaccgcta aatttgacga atctgttgac gtatctttca
      781 acttgggcgt tgattctcgt aaatctgacc aagttatccg tggttcagtc gttctgccta
      841 aaggtactgg taagacaacc cgtgtggctg tatttactca aggtgtaaat gcagaagctg
      901 ctaaagaggc tggtgcggat gtcgtcggtt tcgaagattt ggctgctgaa atcaaagcag
      961 gcaatctgaa ctttgatgtt gttattgctt ctcctgatgc aatgcgtatc gttggtcaat
     1021 tgggtaccat cttgggtcct cgtggtctga tgccaaaccc taaaataggt acggttaccc
     1081 ctaatgttgc cgaagcggta aaaaatgcaa aagcaggtca agtgcagtac cgtaccgaca
     1141 aggcaggtat tgttcatgca acgattggtc gtgcttcatt cgctgaagct gatttgaaag
     1201 agaactttga tgcgttgctg gacgctatcg ttaaagccaa acctgctgct gctaaaggtc
     1261 agtacctgaa aaaagttgct gtatccagca ctatgggttt aggtgttcgc gttgatacat
     1321 caagcgtgaa taactaatct aagaaatttt caagcaactc tgtttttgag ttgcttgaat
     1381 ttgggctact taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta
     1441 actgccctac gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt
     1501 ttaggttacc gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta
     1561 gaccttgagt ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat
     1621 tgctaatgct caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac
     1681 tgagcttcgt gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatactct
     1741 ggctcgtcgc gcagtgcaag gtacttcatt tgtagaattg gccgatcaaa tggttggtcc
     1801 gttggtttac gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc
     1861 gaaaaaagat gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc
     1921 tgctcaggtt gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaaactgtt
     1981 gttcgttatg caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa
     2041 aaaagccggc gaagaagctg cttaatcgat tttgtttctg ttaatcaatt attttttaat
     2101 acaatatttg gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg
     2161 ttctttgacc gtaatggaat tgaatgacct ggttaaagct tttgaagaaa aattcggtgt
     2221 ttctgctgct gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga
     2281 aaaaaccgaa tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa
     2341 agttgtccgt gcaattactg gtttgggtct gaaagaagct aaagacatcg ttgacggcgc
     2401 acctaaaacc attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact
     2461 ggaagcagca ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca
     2521 gttttctgcc agccttattt tgcttcttaa aataagcatt aagtattgtt tacatttatt
     2581 tgcatagttt ttatcaaatc attgcaaata aatgtaaata tcagattgat gcgtaccgtt
     2641 gttttcagac ggcctattat tgaaaattac ttttcggagt gtgtatgaac tattcgttta
     2701 ccgagaaaaa acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt
     2761 ttctactggc aacccaaatt gattcttatg cgaaattttt gcagctggaa aatgcttttg
     2821 acaaacgtac cgatgacggt ctgcaagcgg catttaattc tattttcccg attgtgagcc
     2881 acaacggtta tgctcgtctg gagtttgtat attacacgct gggcgagcct ttgttcgata
     2941 tccctgaatg tcaattgcgc ggaatcactt atgctgcccc tctgcgcgcg cgtatccgtt
     3001 tggtgatttt ggataaggaa gcgtctaaac cgacggtaaa agaagttcgt gaaaacgaag
     3061 tgtatatggg cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag
     3121 agcgtgtgat tgtttcccag ttgcaccgtt cgcccggtgt gttcttcgag catgacaaag
     3181 gtaagacaca ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt
     3241 catggttgga ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc
     3301 gtaaaatgcc ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg
     3361 atattttcta cgacaaagaa acgttttatt tgtcttcaaa cggtgttcaa accgatttgg
     3421 tcgcaggccg tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg
     3481 tattggttgc caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag
     3541 gcctgacccg tttggatgta gaacaggaaa gcctgctggg caaagcattg gctgccgatt
     3601 tgattgattc ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt
     3661 tgttggccaa atttgatatt aacggcgtaa aagaaattac gaccctttac atcaatgagt
     3721 tggatcaggg tgcttatatt tccaatacct tgcgcacgga tgagactgcc ggccggcagg
     3781 cggcgcgtgt tgcgatttac cgtatgatgc gtccgggcga accgcccacc gaagaggcgg
     3841 tcgagcaatt gtttaaccgc ttgttcttca gtgaagacag ctacgatttg tcccgcgtag
     3901 gccgtatgaa atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact
     3961 cttggtacgg ccgcctgttg aacgagacgt ttgccggtgc tgccgacaaa ggcggttatg
     4021 tcctgagcgt cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc
     4081 atggcgaagt ggacgatatc gatcacttgg gcaaccgtcg agtacggtcg gtaggcgagc
     4141 tgactgaaaa ccaattccgc agcggtttgg ctcgtgtgga acgtgccgta aaagaacgtt
     4201 tgaatcaggc ggaatcagaa aacttgatgc cgcacgactt gattaatgcg aaacccgttt
     4261 ctgccgccat caaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca
     4321 atcccttgtc tgaagtaacc cataaacgcc gtgtatctgc attgggcccg ggcggtttga
     4381 cccgcgaacg tgccggcttc gaagtgcggg acgtgcatcc gacccactat ggacgtgtat
     4441 gtcctattga aacgcctgaa ggtccgaaca tcggtttgat caactcattg tctgtttacg
     4501 cgcgcaccaa tgattacggt ttcttggaaa cgccttaccg ccgcgttatt gacggcaaag
     4561 taaccgagga aattgattac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg
     4621 cgaatgccga tttggattca gacggcaatc tgattggcga tttggttacc tgtcgtgaaa
     4681 aaggcgaaac cattatggca acgcccgacc gcgtccaata tatggacgtg gcaaccggtc
     4741 aggtggtatc cgttgcggca tccctgattc cgttcttgga acacgatgac gcgaaccgcg
     4801 cattgatggg tgccaacatg caacgtcagg cagtgccttg tctgcgtcct gaaaaaccga
     4861 tggtcggtac cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc
     4921 gccgaggcgg cgtggtcgag tatgtcgatg ccaaccgcgt tgtgatccgt gtccatgacg
     4981 acgaagcgac tgccggcgaa gtgggtgtcg atatttacaa tttggttaaa ttcacccgtt
     5041 ccaatcaatc taccaacatc aaccaacgtc cggctgtcaa agcaggcgat gttttgcaac
     5101 gcggcgattt ggtggccgac ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa
     5161 atatgaccat cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt
     5221 ccgaaaaagt ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg
     5281 ttgcccgcga taccaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg
     5341 agcgtatgca aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag
     5401 ccggcgatgt gttggtaggc aaggtaacgc ctaaaggcga aacccagctg acgccggaag
     5461 aaaaactgct gcgcgccatc ttcggcgaaa aagcgtctga cgtaaaagat acttcattgc
     5521 gtatgcctac cggtatgagc ggtacggtta tcgacgttca agtctttacc cgcgaaggca
     5581 tccaacgcga caaacgcgcc caatccatta tcgattccga attgaaacgc taccgtttgg
     5641 atttgaacga ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gagcgtatga
     5701 ttgtcggtca gaaagccaac ggcggcccga tgaagctggc caaaggcagc gaaatcacga
     5761 ccgaatatct ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ttgaccgatg
     5821 aagatttggc caagcagttg gaactgatta aattgagcct gcaacaaaaa cgcgaagaag
     5881 cggatgagtt gtatgagatc aagaagaaaa aactgaccca aggcgacgag ctgcaacccg
     5941 gcgtacaaaa aatggtgaaa gtctttatcg ccatcaaacg ccgtctgcaa gccggtgaca
     6001 aaatggcggg ccgccacggt aataaaggtg tggtatctcg cattctgcct gtggaagaca
     6061 tgccgtacat ggcggacggc cgtcctgtgg acatcgtact gaacccgttg ggcgtacctt
     6121 cccgtatgaa catcggtcag attttggaag ttcacttggg ttgggcggca aaaggtatcg
     6181 gcgagcgcat cgaccgtatg ctgaaagagc gacgcaaagc cggcgagctg cgcgagttct
     6241 tgaacaaact ctacaacggc agcggtaaga aagaagattt ggacagcctg actgatgaag
     6301 aaatcatcga attggcttcc aatctgcgta aaggtgcatc tttcgcctct cctgtattcg
     6361 acggtgcgaa agagtctgaa atccgcgaaa tgttgaactt ggcttaccca agcgaagatc
     6421 ctgaggttga gaaactgggc ttcaacgaca gtaaaactca aatcacgctg tatgacggcc
     6481 gttcaggcga agcatttgac cgcaaggtta cagtcggtgt gatgcactat ctgaaactgc
     6541 accacttggt tgacgaaaaa atgcacgcgc gttctaccgg cccgtacagt ctggttactc
     6601 aacaacctct gggcggtaaa gctcagtttg gcggccaacg tttcggtgag atggaggttt
     6661 gggcattgga agcatacggc gcggcataca cgctgcaaga gatgctgact gtgaagtctg
     6721 acgacgtgaa cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg
     6781 atgccggtat gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggtttgg
     6841 atatcgattt ggaacgctac tgatacgggt ttcagacggc ataaggggag ctgttctgca
     6901 ggtatgcggg gcagcccgac aatgtttaaa aacgaaatgc cgtctgaaag cactgtacct
     6961 ttatccatat cgaaaatccg ccacgcggta aaaatatttc cttcaaggag cagaaatgaa
     7021 tttgttgaac ttatttaatc cgttgcaaac cgcaggtatg gaagaagagt ttgatgccat
     7081 caaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg aagttaaaaa
     7141 acctgaaacc atcaactacc gtacgttcaa acccgagcgc gacggcttgt tctgcgccaa
     7201 aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac gattgaaatt
     7261 taaaggcgta acctgtgaaa aatgtggcgt agaagttacc ctgtccaaag tgcgccgcga
     7321 acgcatgggc catatcgaat tggctgcacc cgttgcccat atttggttct tgaaatccct
     7381 gccttcccgc ttgggtatgg tattgaacat gacgttgcgt gatatcgaac gcgtattgta
     7441 ctttgaagca ttcgtggtga ccgatcccgg catgactccg ttgcaacgtc gtcaattgct
     7501 gactgaagac gattactaca acaaactgga cgaatacggc gacgacttcg atgccaaaat
     7561 gggtgcggaa ggtatccgcg aattgttgcg aaccttggat gtagcaggcg aaatcgaaat
     7621 cctgcgccaa gagcttgaat cgaccggttc tgacaccaaa atcaaaaaaa tcgctaaacg
     7681 cttgaaagta ttggaagcct tccatcgttc cggtatgaag ctggagtgga tgattatgga
     7741 tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccgttggacg gcggccgttt
     7801 tgccacttcc gatttgaacg atttgtaccg ccgcgtcatc aaccgcaaca accgtctgaa
     7861 acgcctgctg gaactgcatg cgcctgacat catcgtccgc aacgaaaaac gtatgttgca
     7921 agaagcggtt gactcgctgt tggataacgg ccgtcgcggt aaagccatga ccggagccaa
     7981 caaacgtccg ctgaaatcgt tggcagatat gattaaaggt aaaggcggcc gcttccgtca
     8041 aaacctgttg ggcaaacgtg tggactactc cggccgttcc gtgattaccg taggcccata
     8101 cctgcgtctg caccaatgcg gtttgccgaa aaaaatggcg ttggaactgt tcaaaccatt
     8161 tattttccac aaattggaaa aacaaggttt ggcgtctacc gttaaagctg cgaaaaaatt
     8221 ggtagaacaa gaagtacctg aagtatggga tatcttggaa gaagtcatcc gcgaacatcc
     8281 gattatgttg aaccgtgcgc cgaccctgca ccgtttgggt attcaagcgt tcgagcctat
     8341 tctgatcgaa ggtaaagcga ttcagttgca cccattggtg tgtgccgcat ttaatgccga
     8401 ctttgacggt gaccaaatgg cagtacacgt tccattgagc ttggaagcgc aaatggaagc
     8461 acgcacgctg atgctggctt caaacaacgt attgtctcct gccaacggcg aaccgattat
     8521 cgtaccttcc caagacatcg tattgggtct gtactacatg acccgcgacc gtatcaatgc
     8581 caaaggcgag ggcagcctgt ttgccgatgt gaaagaagtg catcgtgcat accataccaa
     8641 acaggtcgag ctgggtacaa aaatcaccgt acgtctgcgc gaatgggtga aaaacgaagc
     8701 aggtgagttt gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg cattgttgag
     8761 cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga agaaaaaaga
     8821 aatttccaag ctgattaacg catcgttccg cctgtgcggc ttgcgcgata cggttatctt
     8881 cgccgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta tttccattgc
     8941 cgttgacgat atggaaattc caaaagaaaa agcggccttg ctggctgaag ccaatgccga
     9001 agttaaagaa atcgaagacc aataccgtca aggtttggtt accaacggcg aacgctacaa
     9061 caaagtggtc gatatttggg gtcgtgccgg cgataagatt gctaaagcga tgatggacaa
     9121 cctgtccaaa caaaaagtta tcgaccgtga cggcaacgaa gtcgatcaag agtcattcaa
     9181 ctccatttat atgatggcgg actccggtgc ccgtggttct gcggctcaga ttaaacagtt
     9241 gtccggtatg cgcggtttga tggcgaaacc tgacggatcg attattgaaa cgccgattac
     9301 ctcaaacttc cgcgaaggtc tgaccgtatt gcaatacttt attgcgaccc acggtgcgcg
     9361 taagggtttg gcggataccg cgttgaaaac cgcgaactcc ggttacctga cccgtcgtct
     9421 ggtggatgta actcaagact tggtcgttgt tgaagacgat tgcggtactt cagacggctt
     9481 tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg atcgtatttt
     9541 gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagc ggcgagactt tggttgaagc
     9601 cggtacgttg ttgactgaaa aactggtgga tatgattgac caatccggtg tcgatgaagt
     9661 caaagtccgt actccgatta cttgtaaaac ccgccacggt ctgtgcgcgc actgttacgg
     9721 ccgcgacttg gcgcgcggca aactggtcaa tgccggcgag gcagtcggcg tgattgcggc
     9781 tcagtctatt ggtgaaccgg gtacccagtt gaccatgcgt acgttccaca tcggtggtgc
     9841 ggcatcccgt gcggcagcag ccagccaagt cgaagccaaa tccaacggta cggcacgttt
     9901 cagcagccaa atgcgttatg ttgccaacaa caaaggcgag ttggttgtca tcggccgttc
     9961 ttgcgaagtc gtgattcacg atgacatcgg ccgtgaacgc gaacgccaca aagtacctta
    10021 cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa ccttggcaac
    10081 ttgggatccg catacccgtc cgatgattac cgaacatgca ggtatggtga aattcgagaa
    10141 catggaagag ggcgttaccg ttgcaaaaca aaccgatgat gtaaccggtt tgtccacttt
    10201 ggtagtgatt gacggtaaac gccgttcctc tagtgcttcc aaactgctgc gtccgactgt
    10261 gaaactcttg gacgaaaacg gcgtggaaat ctgtattccc ggtacgtcca ctccggtttc
    10321 gatggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa tcggtaaagg
    10381 cgacgtattg gcgcgtattc cgcaagcctc ttccaaaacc cgcgacatta ccggcggtct
    10441 gccgcgcgtt gccgagctgt ttgaagcacg cgtgccgaaa gatgccggta tgctggcgga
    10501 aattaccggt accgtttcct tcggtaagga gaccaaaggc aaacaacgtc tgattatcac
    10561 tgacgtggac ggtgtagcat acgagacttt gatttccaaa gagaaacaaa ttctggtaca
    10621 tgacggtcaa gtggtaaacc gaggcgaaac cattgtggac ggcgcggtcg atccgcacga
    10681 tattctgcgt ctgcaaggta tcgaagcact ggcacgctac attgtccaag aggtgcaaga
    10741 ggtttaccgc ctgcaaggtg tgaagatttc cgataaacac atcgaagtca tcatccgtca
    10801 aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggtttatta ccggagagca
    10861 ggtcgaacgc ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag gcaaagagcc
    10921 ggcacgttac gaaaacatat tgctgggtat taccaaagcc tccctgtcca ccgacagctt
    10981 catttctgcc gcatcgttcc aagaaaccac ccgtgtgttg accgaagctg ccatcatggg
    11041 caaacaagac gaactgcgcg gtctgaaaga gaatgtcatc gtcggtcgcc tgattcctgc
    11101 cggtaccggt ttgacttatc accgcagccg tcatcagcaa tggcaagggg tagagcagga
    11161 gactgctgaa acccaagtaa cggatgaata atcttcggcg cgttcattca ataaaaaacc
    11221 gcaagccttg agcttgcggt ttttctttgt ccgattaagg caaaaacaag cgttttcgtc
    11281 attttgaggc gtgtggatat aagcccgata agtgtcccat atcttcgtgc cgtcaaatta
    11341 tgcatcgaat agaacgagtt aaatccgaag gttattgtgt taaatattgc aaaa
//
LOCUS       DAXFHF010000063        11178 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXFHF010000063 DAXFHF010000000
VERSION     DAXFHF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.063"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(507..1649)
                     /locus_tag="ACFIRM_001693"
     CDS             complement(507..1649)
                     /locus_tag="ACFIRM_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC3029276.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1955..2218
                     /gene="rpsT"
                     /locus_tag="ACFIRM_001694"
     CDS             1955..2218
                     /gene="rpsT"
                     /locus_tag="ACFIRM_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC3029277.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2378..3526
                     /locus_tag="ACFIRM_001695"
     CDS             2378..3526
                     /locus_tag="ACFIRM_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC3029278.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3668..4369
                     /locus_tag="ACFIRM_001696"
     CDS             3668..4369
                     /locus_tag="ACFIRM_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC3029279.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4427..6235
                     /gene="aspS"
                     /locus_tag="ACFIRM_001697"
     CDS             4427..6235
                     /gene="aspS"
                     /locus_tag="ACFIRM_001697"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC3029280.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGIAGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            6385..6669
                     /locus_tag="ACFIRM_001698"
     CDS             6385..6669
                     /locus_tag="ACFIRM_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3029281.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6836..8728
                     /gene="speA"
                     /locus_tag="ACFIRM_001699"
     CDS             6836..8728
                     /gene="speA"
                     /locus_tag="ACFIRM_001699"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC3029282.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8827..9750
                     /gene="speB"
                     /locus_tag="ACFIRM_001700"
     CDS             8827..9750
                     /gene="speB"
                     /locus_tag="ACFIRM_001700"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC3029283.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLVDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9948..>11178
                     /locus_tag="ACFIRM_001701"
     CDS             9948..>11178
                     /locus_tag="ACFIRM_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC3029284.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFL"
BASE COUNT         2687 a         2815 c         2909 g         2767 t
ORIGIN      
        1 ggtccgtcgg cataggaact tatcggataa aacggtttct tgagatttga cattctagat
       61 tcccactttc gtgggaatga cgggatgcgg atttccgtgc ggatgcgttc tagattcccg
      121 ctttcgcggg aatgacggga ttccggtttg cggattgatt tgaagttgca aaatcccaac
      181 gggccggatt cccgcctgcg cgggaatgac ggcggaaggt tttttgtctt tcctgattat
      241 agtggattaa caaaaatcag gacaaggcga ggaagccgca gacagtacaa atagtacgga
      301 accgattcac ttggtgcttc agcaccttag agaatcgttc tctttgagct aaggcggggc
      361 aacgccgtac cggtttttgt taatccgcta taaatgtccg caatctgaaa tcccggccgt
      421 gggggcgacg gtataaatat ttctatttca atccaatata aaatgccgtc cgaacatcgt
      481 tcggacggca tttttaatgc ttcgaatcag ttggtgccga ctttgatttt ctgccacagg
      541 ctgaccgaca gttttttcgc atccgtgctc atttgcggca tgacgaaacc gtctttcata
      601 tcctgctcgt tcgggaagat ggaacgggtg ttcaccaact cggcaggcat tttttcgcgc
      661 gccggtttgc tggcgggggc gaaggttacg gcgatgccgt ttttcgccgc gatttcgggg
      721 tcgagcgtgt agttgatgta tttgtgggcg ttggcgacgt ttttcgcatc ggcgggaatc
      781 agccaagact caatccagaa gcccatacct ttcggtgtca gcacttcgat gccgacgttg
      841 tttttcactt cttcggaacg cgctttcgcc aagttcaaat cgccgccgtt gcctgccgcc
      901 aggcagatgt cgccgcgcgc cagctcgtcg atgatggacg ggctgaaacg tttgacgtcc
      961 ggacggatag acttcaacac ttccgccgcc gccttcaaat cttcaggatt cgaacctttg
     1021 gggtctttgc ccaagtagtt cagcaaaatc gggaacattt cgctcggggt gtcccacagg
     1081 gcgatgccgc aggatttcag cttgcgggtg tattcgggtt tgaacagcaa atcccagccg
     1141 ttttcgggca gcttgccgcc caaaagctct ttgcccttcg ccgtaatcgc aaccgtgttc
     1201 acgccggaga aataggggac ggcatactgg ttgcccgggt cggcggtttc cagcattttc
     1261 aagagttcgg gatcgatgtt tttatagttg ggaatcaggt ctttgttgac tttttgatac
     1321 gcgcccgcct cgatttggcg cggcaggaag gcgatgcccg gcacaaccaa atcgtaaccg
     1381 gatttgccgg tcagcatttt ggcttccagc gtttcgttgt tttcgtacaa gtcgtaagtc
     1441 agcttcagat tgttggcttt tttaaagtct tcgaccgtac tctcgtcaac ataattcgac
     1501 cagttgtaga tgttcagagt atcggtggca gcggcttcgg cattggcagc aggtgcgctg
     1561 cctgcttgag gctgcacggc gtttttttcg ctgccgccgc aggctgccag agacagcgcg
     1621 gccaaaacgg ctaatacgga ttttttcata cgggcagatt cctgatgaaa gaggttggaa
     1681 aaaaaagaaa accccgcgcc ccataaacac cccggcgcaa ggtttgggca ttgtaaagta
     1741 aatttgtgca aactcaaagc gatattggcc tgattttcct aaaaaattac cctgtttcca
     1801 aaaggagggg ggaaacggcc gcccgatttt gccgtttttt tgcgccgtca gggtgtccga
     1861 cgggcggata gagaaaaaag gcttgcatat aatgtaaacc ccctttaaaa ttgcgcgttt
     1921 acagaattta tttttctttc aggagattcc aaatatggca aacagcgcac aagcacgcaa
     1981 acgtgcccgc cagtccgtca aacaacgcgc ccacaacgct agcctgcgta ccgcattccg
     2041 caccgcagtg aaaaaagtat tgaaagcagt cgaagcaggc gataaagctg ccgcacaagc
     2101 ggtttaccaa gagtccgtca aagtcatcga ccgcatcgcc gacaaaggcg tgtttcataa
     2161 aaacaaagcg gctcgccaca aaagccgtct gtctgcaaaa gtaaaagcac tggcttgatt
     2221 tttgcaaaac cgccaaggcg gttgatacgc gataagcgga aaaccctgaa gcccgacggt
     2281 ttcggggttt tctgtatttc gggggtaaag ttcgaaatgg cggaaagggt gcggtttttt
     2341 atccgaatcc gctataaaat gccgtttgaa aaccaatatg ccgacaatgg gggcggagat
     2401 gaatacacga aatatgcgct atattctttt gacaggactg ttgccgacgg catccgcttt
     2461 tggagagacc gcgctgcaat gcgccgcttt gacggacaat gttacgcgtt tggcgtgtta
     2521 cgacaggatt tttgcggcac agcttccgtc ttcggcaggg caggaagggc aggagtcgaa
     2581 agccgtactc aatctgacgg aaaccgtccg cagcagcttg gataagggcg aggcggtcat
     2641 tgttgttgaa aaaggcgggg atgcgcttcc tgccgacagt gcgggcgaaa ccgccgatat
     2701 ctatacgcct ttgagcctga tgtacgactt ggacaaaaac gatttgcgcg ggctgttggg
     2761 cgtacgcgaa cacaatccga tgtaccttat gccgttttgg tataacaatt cgcccaacta
     2821 tgccccgagt tcgccgacgc gcggtacgac tgtacaggaa aaattcggac agcagaaacg
     2881 tgcggaaacc aaattgcagg tttcgttcaa aagcaaaatt gccgaaaatt tgtttaaaac
     2941 ccgcgcggat ctgtggttcg gctacaccca aagatccgat tggcagattt acaaccaagg
     3001 caggaaatcc gcgccgttcc gcaatacgga ttacaaacct gaaattttcc tgacccagcc
     3061 tgtgaaggcg gatttgccgt tcggcggcag gctgcgtatg ctcggtgcgg gttttgtcca
     3121 ccagtccaac ggacagagcc gtcccgaatc gcgttcgtgg aacaggattt atgccatggc
     3181 aggcatggaa tggggcaaat tgacggtgat tccgcgcgtg tgggtgcgtg cgttcgatca
     3241 gagcggcgat aaaaacgaca atcccgatat tgccgactat atggggtatg gcgacgtgaa
     3301 gctgcagtac cgcctgaacg acaggcagaa tgtgtattcc gtattgcgct acaaccccaa
     3361 aacgggctac ggcgcgattg aagccgccta cacgtttccg attaagggca aactcaaagg
     3421 cgtggtacgc ggattccacg gttacggcga gagcctgatc gactacaacc acaagcagaa
     3481 cggtatcggt atcgggttga tgttcaacga ctgggacggc atctgaatac cgtttcaaac
     3541 ggcatatcaa gttgccgtgc cgtctgaagc cgccggcggt ttggcggggc gggataaaaa
     3601 attcgtttga atccgggcaa acgcgtataa tgtgcggttt gtacggactt tgtcggaatc
     3661 aagcaagatg acggaacctg cggccgaagg cggcaaagct gccaaggcgt taaaaaaata
     3721 tctgattaca ggcattttgg tctggctgcc gattgcggta acggtttggg tggtttccta
     3781 tatcgtttcc gcgtccgacc agcttgtcaa cctgctgccg aagcaatggc ggccgcaata
     3841 tgttttgggg tttaatatcc ccgggctcgg cgttattgtt gccattgccg tattgtttgt
     3901 aaccggatta tttgccgcaa acgtgttggg tcggcagatt cttgccgcgt gggacagcct
     3961 gttgggtcgg attccggttg tcaaatccat ctattcgagt gtgaaaaaag tatccgaatc
     4021 gctgctgtcc gacagcagcc gttcgtttaa aacgccggta ctcgtgccgt ttccccaatc
     4081 gggtatttgg acaatcgcat tcgtgtccgg tcaggtgtcg aatgcggtta aggccgcatt
     4141 gccgcaggat ggcgattatc tttccgtgta tgtcccgacc acgcccaacc cgaccggcgg
     4201 ttactatatt atggtaaaga aaagcgatgt gcgcgaactc gatatgagcg tggacgaagc
     4261 gttgaaatat gtgatttcgc tgggtatggt catccctgac gacctgcccg tcaaaacatt
     4321 ggcaggacct atgccgcctg aaaaggcgga gttgcccgaa caacaataaa gctgccgttc
     4381 agacggcatt ttctgttttc agtttaaatc aataaaaggt gattttatgc gtaccaacta
     4441 ttgcggcctg atcagcgagc aatacttagg ccaaaccgtt accgtcaaag gctgggtaca
     4501 ccgccggcgc gaccacggcg gtgtgatttt tatcgacctg cgcgaccgcg aaggcatcgt
     4561 ccaagtcgtg atcgatcccg acacgcccga agcgtttgcc gctgccgatt ccgcccgtaa
     4621 cgaatacgtt ttgggcattg ccggccgcgt acgcaaccgt cccgaaggca cgaccaacga
     4681 taaaatgatt tccggcaaaa tcgagattct tgccaaagaa atcgaagtgt tgaacgccgc
     4741 cgctacgccg ccgttccaaa tcgacgacga aaacatcagc gaaaacgtgc gcctgaccaa
     4801 ccgcgttatc gacctgcgcc gccctgtgat gcagcgcaac ctgcgcctgc gttaccaagt
     4861 cgctacgggc gttcgccgtt atctggacgc gcaaggcttc atcgacatcg aaacgccgat
     4921 gctgacccgc tccactccgg aaggcgcgcg cgactacctc gtaccgagcc gcgttcatcc
     4981 gggcgagttt ttcgcgctgc cgcaatcgcc gcaattattc aaacaactgt tgatggtggc
     5041 gggtttcgac cgttactacc aaatcaccaa gtgcttccgt gacgaagact tgcgcgccga
     5101 ccgccaacct gaattcactc aaatcgactt ggaaacctcg ttcctgaacg aggatgaaat
     5161 catggacatc actgaaggca tggccaaaca agtcttcaaa gatgctttga acgtagattt
     5221 gggcgacttc ccacgcatgc cttattctga agccatgttc tactacggct ctgacaaacc
     5281 ggacatgcgc atcaacttaa aattcaccga gttgaccgac ctgatgaaaa cggaagaatt
     5341 caaagtcttc cgtggcgcag ccgacatgaa aggcggccgc gtagtcgctc tgcgcgtgcc
     5401 gaacggcgcc aaattcagcc gcaaagaaat cgacgaatac actaaatttg tcggcatcta
     5461 cggcgcgaaa ggtctggcat acatcaaagt aaacgatgcg ggcaacctct ccaacggcga
     5521 agacagcggc ctgcaatctc caatcgtaaa attcttgtct gaaaacgccc tgaaagaaat
     5581 catcgagcgt accgccgcgc aaaacggcga catcatcttc ttcggtgcgg acaaagccaa
     5641 agtcgtgaac gaagccgtcg gcgcattgcg tatcaaagtc ggcttggaac acggtaaaga
     5701 caacggctac ttcacagacg aatggaaacc tttgtgggtt gtcgatttcc cgatgttcga
     5761 atacgacgaa gaagccgacc gctacgttgc cgtacaccat ccgtttaccg ctcctaaaga
     5821 aggtcatgaa gacctgatgg tttccgatcc tgcaaactgc ctggcccgtg cctacgatat
     5881 ggtattgaac ggctgggaaa tcggcggagg ctctatccgt atccaccgtg cagacgtaca
     5941 agagaaagtg tttgccgcgc tgaaaatcag ccctgaagag caacaagaga aattcggctt
     6001 cctcttggac aacctgaaat tcggtgcgcc tcctcacggc ggccttgcat tcggcctcga
     6061 ccgtctggta acgctgatga ccggtgccga atccatccgc gacgtgattg ccttcccgaa
     6121 aacacaacgc gcccaatgcc tgctgaccga tgcgcccaac agcgtggacg ataagcagtt
     6181 gcgtgaattg agcctgcgtc tgcgtcaaaa agcagttgaa acaaaagaag cataaggaaa
     6241 acagagccgt ttgacggctc tgttttttca gacggcattt acgcttcttg acttccctct
     6301 aattcaaacc taattttgct gtgttttaat ggcggtattg aaaaacacat cttgttcaat
     6361 caaccgataa aaaaaggact gaaaatgaaa aaactgttat tggccgccgt tgtttcccta
     6421 aatgccgcaa ccgcatttgc cggcgactct gccgagcgtc agatttacgg cgatccccat
     6481 tttgaacaaa accgcacaaa agccgtgaaa atgttggaac agcgcggtta tcaggtttac
     6541 gatgtcgatg ccgacgacta ctggggcaaa cctgttttgg aagtggaagc ctataaagac
     6601 ggccgcgaat acgacatcgt gttgtcttac cccgacctga aaatcatcaa agagcagctc
     6661 gatcgctgac tcctttgatg gaaagatgaa ccaaaatgcc gtctgaagcg ttcagacggc
     6721 attttgcctg ttcctcatca ggtatgaggc aggcttttct tattaaaaaa atgacatttc
     6781 acgctgattt gttataatca ttccttttca acacgacaga cggagcaggt ttattatgcc
     6841 tatccttacc atccgtgaag tgtgcaacat taatcattgg agcataggtt attatgatgt
     6901 tgacgattcc ggcgaaatca tcgtccgccc caatccctcg caacacaatc aaactgtttc
     6961 actgcaaaaa ctgactgaag ccgtgcaaca aaaacatcag gcgcgcctgc ctgttttgtt
     7021 ttgttttccg caaatcctcg aacaccgcct ccgcgacatt aaccgcgcct ttcagacggc
     7081 acgggaagag tgcggctata agggcggtta ttgtttggtt taccctatca aggtcaacca
     7141 acaccgccgc gtcatcgagt cgcttatgtc aagcggacaa ccgcatggtt tggaagccgg
     7201 ttctaaagcc gaactgatgg cggttttggc acacgccggc acccggcaaa cattaatcgt
     7261 ctgcaacggc tataaagacc gtgaatatat ccgtttcgcc ttgatgggcg aaaaactggg
     7321 gcatcaggtt tatttggtga ttgagaagct gtccgaaata caaatggtat tggaagaggc
     7381 ggaaaaactc ggcatcaagc cccgtttggg tgtgcgcgcc agactggctt cccaaggttc
     7441 gggaaaatgg cagtcttcgg gtggggaaaa atcaaaattc ggcttgtcgg cttcccaagt
     7501 tttgcaactg gtcgatattt tgaaacaaaa aaacaggctg gactgcctgc agcttttgca
     7561 tttccatttg ggctcgcagc ttgggaacat ccgtgatgtt gccacaggtg tacacgaatc
     7621 ggctcggttt tatgttgagt tgcacaaact gggggtaaat atccgctgtt ttgatgtagg
     7681 tggcgggctt ggcgtggatt acgaaggaaa ccgcacacaa tcggattgtt ccgttaatta
     7741 cagcctgaac gaatatgccg ccacagtcgt atggggcatc agtcaggctt gtctcgaaca
     7801 cgggctgccg catccgacaa tcattaccga gagcgggcgc ggcattaccg cacatcacgc
     7861 cgttttggtt gctaatgtta taggcgttga acgttacaaa ccgcgtcggc tggatgcgcc
     7921 atcgcccgaa gcaccgcgtg tgttgcacag tatgtgggaa acttggacgg atatttccgc
     7981 ctcgcgggaa aaacgttcct tacgcagctg gatacacgaa gggcagtttg atcttgccga
     8041 tgtgcataat cagtataatg tcgggctgtt gagtttggcg caacgtgcgt gggcggagca
     8101 actgtattta aatatctgtc atgaagtcgg cgaattgttt aatgaaaaac accggtctca
     8161 ccgaaccatt attgacgaat tgcaagaacg ttttgccgat aagctgtatg tcaatttctc
     8221 actcttccaa tctttgcccg atgcttgggg catagatcaa cttttccctg tttgtcctat
     8281 taccggtttg aatgaaccga ttgcgcgccg cgccgtgttg ttggacatta cctgcgattc
     8341 agacggtacg attgaccact acatcgacgg cgacggcatc gccggtacga tgcctatgcc
     8401 tgattatccc gaagaagagc cgccgttttt aggctttttt atggtgggag catatcagga
     8461 aatactcggc aatatgcaca atcttttcgg cgacactgcc actgctgatg ttgttgtaag
     8521 ggaagacgga caatttaccg tcatcgatta cgatgaagga aacaccgttg ccgatatgct
     8581 cgaatacgtt tatcaagatc cgaaagagct gatgaaacgc tatcgcgaac aaatcgaaca
     8641 ttcagacctt cctgcctcgc aggctatgtc tttcttaaaa gaactcgaag cggggcttaa
     8701 tggttatacc tatttggaag acgaatagac gcatcaaggt atcggatatg tcgtctgaag
     8761 cccgattttc ttactcaaac accaatcatc acgaccgatt gaaaccaatt acaaggaatc
     8821 attacgatgc aatacagcac actggcagga caaaccgaca actccctcgt ttccaataat
     8881 ttcgggtttt tgcgcctgcc gcttaatttt atgccgtatg aaagtcatgc cgattgggtt
     8941 attaccggcg tgccttatga tatggcggtt tcagggcgtt ccggcgcgcg tttcggtcct
     9001 gaagccatcc ggcgcgcctc cgtcaacctc gcttgggagc accgcaggtt tccgtggaca
     9061 tttgatgtgc gcgaacgcct gaacattatt gattgcggcg acttggtttt ttcttttggc
     9121 gacagcaggg attttgtcga aaaaatggaa gcgcacgccg gcaaattact ttctttcggc
     9181 aaacgctgtt tgagtttggg cggcgaccat ttcattaccc tcccgttgtt gcgcgcccac
     9241 gcccgctatt tcggcaaact cgcactgatt cattttgacg cgcacaccga cacctacgac
     9301 aacggcagcg aatacgacca cggcacgatg ttttataccg cccccaagga aggcctcgtc
     9361 gacccgtccc gttccgtaca aatcggcata cgcaccgaac acagtaaaaa attgcctttt
     9421 actgtgttgt ccgcccccaa agtcaatgaa gacagtgttg aagagaccgt ccgtaaaatc
     9481 aaagaaaccg tcggcaatat gcccgtttac ctgactttcg acatagactg tctcgacccg
     9541 tcgttcgccc ccgggaccgg tacgcccgta tgcggcggct tgagcagcga cagggcatta
     9601 aaaatcctac gtgggctgac ggatctcgac atcgtcggta tggatgttgt agaagttgcc
     9661 ccctcttacg accaatccga cattaccgct ttggccggcg ccacaattgc cttggaaatg
     9721 ctttaccttc aaggtgcgaa aaaggactga acgtccggca tcccccgggt tttcgccgtg
     9781 ccgttcaaac ggcgtattca gtctaatgaa aattcaaata ctgaaacaaa agttgcccgg
     9841 agccgcatat cggaaagacg gtgaaatatc gtaatataac ttataaaaca attagttaaa
     9901 tattattttt ccgatttttc gggacggtct tttttacgga ggtcaatatg atgaaattgg
     9961 gtttcaaacc gatacccctc gccattgccg cagtattgtg cgccctggtt ttggcactgc
    10021 ccgtacccga cggggtcaag cctcaggctt ggacgctgct ggctatgttt gtcggtgtga
    10081 ttgccgccat tatcggcaag gttatgccgt tgggcgcgct gtcgattatt gccgtcgggt
    10141 tggtcgcagt aaccggcgta accgccgaca aaccgggcgc ggcgatgagc gatgcgttga
    10201 gtgcgttcgc caatccgttg atttggctga ttgccatcgc agttatgatt tcgcgcggtt
    10261 tgctcaaaac agggctgggg atgcgtatcg gatatttgtt tatcgccgtt tttggaagaa
    10321 aaacgctggg catcggttac agtctcgctc tttccgaact gctgctggct cccgttaccc
    10381 cttccaatac cgcgcgcggc ggcggcatta tacatccgat tatgcagtcg attgccggca
    10441 gttacggctc caatcccgca aaaggcacag aaggcaagat gggtaaatat ttggctttgg
    10501 tcaattatca ttccaatccc atttcgtcgg ctatgtttat tactgcaact gcccccaacc
    10561 ctttaatcgt caacttgatt gccgaaaatt taggcagtag tttccgtctt tcttgggggg
    10621 cgtgggcgtg ggcaatggct gttcccggcg ttatcgcctt tttcgttatg cctttgattt
    10681 tatatttttt gtatccgcct gaaattaaag aaacgcccaa tgctgttcaa tttgccaaag
    10741 accgtctgag cgagatgggt aaaatgtcgg cagacgaaat cattatggcg gtcattttcg
    10801 gtatcttgct gctgttgtgg gcagatgttc ccgcccttat taccggcaat cacgctttta
    10861 gtatcaacgc caccgccacc gcatttatcg gattaagcct gcttttgctt tccggtgtat
    10921 tgacttggga cgatgttttg aaagaaaaaa gcgcgtggga tacgattatt tggtttggcg
    10981 cattgattat gatggccgca tttttaaata aactcggact gattaaatgg ttctccggag
    11041 tgttggcgga aagtgtcggc ggtttgggcg ttagcggcac ggctgcgggc gtaatcctcg
    11101 tgcttgctta tatgtatgcg cattatatgt ttgccagtac tactgcacat attaccgcta
    11161 tgttcggcgc atttctcg
//
LOCUS       DAXFHF010000064        11073 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXFHF010000064 DAXFHF010000000
VERSION     DAXFHF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11073
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.064"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            184..2557
                     /locus_tag="ACFIRM_001702"
                     /pseudo
     CDS             184..2557
                     /locus_tag="ACFIRM_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(2766..3239)
                     /locus_tag="ACFIRM_001703"
     CDS             complement(2766..3239)
                     /locus_tag="ACFIRM_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC3029285.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGIGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(3370..4725)
                     /gene="yegQ"
                     /locus_tag="ACFIRM_001704"
     CDS             complement(3370..4725)
                     /gene="yegQ"
                     /locus_tag="ACFIRM_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC3029286.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            5051..5839
                     /locus_tag="ACFIRM_001705"
     CDS             5051..5839
                     /locus_tag="ACFIRM_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC3029287.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(5885..6484)
                     /locus_tag="ACFIRM_001706"
     CDS             complement(5885..6484)
                     /locus_tag="ACFIRM_001706"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC3029288.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            6612..7229
                     /gene="gmk"
                     /locus_tag="ACFIRM_001707"
     CDS             6612..7229
                     /gene="gmk"
                     /locus_tag="ACFIRM_001707"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC3029289.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            7287..7493
                     /gene="rpoZ"
                     /locus_tag="ACFIRM_001708"
     CDS             7287..7493
                     /gene="rpoZ"
                     /locus_tag="ACFIRM_001708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC3029290.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            7586..9742
                     /locus_tag="ACFIRM_001709"
     CDS             7586..9742
                     /locus_tag="ACFIRM_001709"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3029291.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTILVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(9809..10993)
                     /gene="coaBC"
                     /locus_tag="ACFIRM_001710"
     CDS             complement(9809..10993)
                     /gene="coaBC"
                     /locus_tag="ACFIRM_001710"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC3029292.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         2679 a         2666 c         2999 g         2729 t
ORIGIN      
        1 gtacagaccc ttaaaaataa tgaaacaaac aaggcctcgg catagatgtt tgcagagacc
       61 tttaattaca cgacacagat ttatttgtac gggttgctgt cttattaaat attaatgatt
      121 atcagaaaat ttattatgaa ttaactgatg gatgaacaat ccattacatc ttgagttgat
      181 aatatgaaac cattacacat gcttcctatt gccgcgctgg tcggcagtat tttcggcaat
      241 ccggtcttgg cagcggatga agctgcaacc gaaaccacac ccgttaaagc agagataaaa
      301 gaagtgcgcg ttaaagacca gcttaatgcg cctgcaaccg tggaacgtgt caacctcggc
      361 cgcattcaac aggaaatgat acgcgacaac aaagacttgg tgcgttactc caccgacgtc
      421 ggcttgagcg atagcggccg ccatcaaaaa ggctttgctg tgcgcggcgt ggaaggcaac
      481 cgtgtcggtg tcagcattga cggcgtgagc ctgcctgatt cggaagaaaa ctcactgtat
      541 gcacgttatg gcaacttcaa cagctcgcgc ctgtctatcg accccgaact cgtgcgcaac
      601 atcgaaatcg cgaagggcgc tgactctttc aataccggta gcggcgcatt gggtggcggc
      661 gtgaattacc aaaccctgca aggccatgat ttgctgttgg acgacaggca attcggcgtg
      721 atgatgaaaa acggttacag cagccgcaac cgcgaatgga caaatacact cggtttcggt
      781 gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc aacgtcgcgg tcatgagacc
      841 gaaagcgcgg gcgagcgtgg ctatccggta gagggtgctg gcagcggagc gaatatccgt
      901 ggttcggcac gcggtatccc tgatccgtcc aaacacaaat accacaactt cttgggtaag
      961 attgcttatc aaatcaacga caagcaccgc atcggcgcat cgtttaacgg ccagcagggg
     1021 cataattaca cgatttgaag agtcttataa cctgaccgct tcttcctggc gcgaagccga
     1081 tgacgtaaac agacggcgca atgccaacct cttttacgaa tggacgcctg attcaaattg
     1141 gttgtcgtct ttgaaggcgg acttcgatta tcagaaaacc aaagtggcgg cggttaacaa
     1201 caaaggctcg ttcccgatgg attattccac ctggacgcgc aactataaat cagaaggatt
     1261 tggacgaaat atacaaccgc agcatggaca cccgattcaa acgttttact ttgcgtatgg
     1321 acagccaacc gttgcaactg ggcggccaac atcgcttgtc gtttaaaact ttcgccagtc
     1381 ggcgtgagtt tgaaaactta aaccgcgacg attattactt cagcggacag atatcccgta
     1441 ctaccagctc gattcaacac cctgtgaaaa ccactaatta tggtttctca ctgtctgatc
     1501 aaatccaatg gaacgacgtg ttcagcagcc gtgcagatat ccgttacgat cataccaaaa
     1561 tgacgcctca ggaattgaat gccgagtgtc atgcttgtga caaaacaccg cctgcagcca
     1621 atacttataa aggctggagc ggatttgtcg gtttggcggc gcaactgaat caggcttggc
     1681 atgtcggtta cgacattact tccggctacc gtgtccccaa tgcgtccgaa gtgtatttca
     1741 cttacaacca cggttcgggt aattggctgc ccaatcccaa cctgaaagcc gagcgcagca
     1801 ccacccacac cctgtctctg caaggccgca gcgaaaaagg tactttggat gccaacctgt
     1861 atcaaaacaa ttaccgcaac ttcttgtctg aagagcagaa gctgaccacc agcggcgatg
     1921 tcggctgtac tcagatgaat tactactacg gtatgtgtag caatccttat tccgaaaaac
     1981 cggaatggca gatgcaaaat atcgataagg cccgaatccg tggtcttgag ctgacaggcc
     2041 gtctgaatgt ggacaaaata gcgtcttttg ttcctgaggg ctggaaattg ttcggctcgc
     2101 tgggttatgc gaaaagcaaa ctgtcgggcg acaacagcct gctgtccaca cagccgccga
     2161 aagtgattgc cggtgtcgac tacgaagccc gagcgaaaaa tggggtgtgt tctcccgcct
     2221 gacttatctg ggtgcgaaaa aggccaaaga cgcgcaatac accgtttatg aaaacaaggg
     2281 ccggggtacg cctttgcaga aaaaggtaaa agattacccg tggctgaaca agtcggctta
     2341 tgtgtttgat atgtacggct tctacaaacc ggctaaaaac ctgactttgc gtgcaggcgt
     2401 atataatgtg ttcaaccgca aatacaccac ttgggattcc ctgcgcggtt tgtatagcta
     2461 cagcaccacc aacgcggtcg accgagatgg caaaggctta gaccgctacc gcgcctcagg
     2521 ccgtaattac gccgtatcgc tggaatggaa gttttaatct agtattattg gattaattgc
     2581 ctcgttgggg attaaagctg tccgaattgt tttcaggaac tcattcggac ggctttttac
     2641 cgtaaatctg tgtgatgggg ttttatattg atacagtatc cgaactcagg gcagggagta
     2701 atgttgcagg attaaaaatg ccgtctgaaa aatgtttcag acggcatttt ttgattgccg
     2761 atatgttaac ggacgacatt ggcgcgcatg accgcgcgga cgtggaacga acctgcctga
     2821 cagtcgtatt gtccgccttg cagcgattgc ttgccaaagt agctgtggaa gccggtaaca
     2881 ctgcgcccgc cgagtttgcc ggcggtctgc tggaatttgc gggcggcatt gattagggcg
     2941 tgttcgcagg cctcggagtc ggatttgcca atgccgtttg ccgcttgccg tgtaactaga
     3001 ttcctgccgt ttgcaaccgg tacgccgccg ccgaagctga cgggaatccc tgattccaat
     3061 acgccggcgg cggtggctgc atttattgcc gcctgaccgt tgctgcaacg gtaaatgatg
     3121 tttttgccgc ctcctgcggt gtctccgcgg ataactttgc atgattctcc ggccggtgtc
     3181 ccggcggatg ccgtgtgcgc gtttttaagc gcgcggccgg attggcaggc tgccagcatc
     3241 agggcggcgg cggagagtat gaggattttt tatgtcggat gtccttgttt gctgtgggaa
     3301 atcattgcca ttgtacagta agattcaaca aaatgccgtc tgaaaacggg gttcggacgg
     3361 cattgtggct caggggttca acacgcgtgc gatgagggct ttttctttgc cctgcatatt
     3421 ggggattttg acctgaatgc cgttgcccgg ggcaacatcg acaggttggc ctttgcgggt
     3481 catttgttcc aatttgatgg tttggttgcc gctcgggtgg atgatttcga gcgaatcgcc
     3541 gacggcgaag cggtttttaa cttcgatggt cgcccagcca ttttcgtcga tttcggtaac
     3601 gtgtccgacg tattggcttt gtttggcggt ggaatggccg ctgaggtagt tttgataatc
     3661 ctgagtttgg tggcgttcga ggaagccgct ggtgtagcct cggttggcaa ggccttcgag
     3721 ttcgctcaac aggctgtaat caaacggacg gcctgcgacg gcatcgtgaa tcgctttgcg
     3781 gtaggactgg gcgacgcggg cgacgtagta gagagacttg gtacggcctt cgactttgag
     3841 gctgtccaca ccgattttgg cgagtttttc gacgacttcg ataccgcgaa gatctttgga
     3901 gttcatgatg taggtgccgt gttcgtcttc cataatcggc atcatctcgc ccgggcggtt
     3961 ggattcttca atcaggaaaa ctttgtcggc gtagggatgg cgtttttgac cgttgatgcc
     4021 ttcaaagttt tggttggctt cttcttgggc tttttcaaag ttgaaacctt gcagaagctg
     4081 ggcatcgcct gcctcgcttt ccgtggcgtt gtggactttg tagtcccaac ggcaggagtt
     4141 ggtgcaggtg ccttggttgg gatcgcggtg gttgaaatag cccgacaaca ggcagcggcc
     4201 tgagtaggcg atgcacaatg cgccgtggat gaagacttcg agttcgatgt cggggcattc
     4261 ttggcggatt tcggcaattt cttccatgcc caattcgcgt gacaggatga tgcgttcgac
     4321 accaatgttc tgccaaaatt tcacgcccca gtagttggtg gtgttcgcct gtacggacag
     4381 atggatcggc atttccggcc atttttcgcg cacggtcata atcaaacccg gatccgccat
     4441 aatcagcgcg tcgggtttca tggcaatcag cggctccatg tcggcaacga aggttttgag
     4501 tttggaattg tgcggcaggg tgttgacggt caaaaagaat tttttgttgc gttcgtgcgc
     4561 ttctttaatg ccttgctcga ggacgtcgag tttggcaaat tcgttgttgc gggcgcgcag
     4621 ggagtaacgc gggctgccgg cgtaaacggc atctgcgccg taatcgtagg cggcgcgcat
     4681 tctttccaat ccgccggcgg gcaataagag ttcgggtgct ttcatcgtgt ttccttttcg
     4741 gttgaaaccc cgccctttag ggcggcagga tcagactttg tttgggaggg gcgtaacccc
     4801 ttccgaatca gggcaacata atagggcggt gctttatgtg tcgtcctgtg tgttgaagca
     4861 tgatttgtat gctttgtaaa aatgtttcag acggcattgc tgcaaatgcc gtctgaaggg
     4921 tgtttgaaga tgggcggatt atccgccttt tcgtgttcgc ggtcaatttt gccgggaaat
     4981 ttataaggat ttatcaagta tttgccttct tgggatacaa tgcggcagtt tgagtgcgag
     5041 agagaatatg atgaatccca aaatcgtgtt tttcgacatt gacgatacgc tgtaccgcaa
     5101 atatacggat accttgcgcc cttccgtgaa aacggcggtg gcggctttgc gcggcaaagg
     5161 tatattgacg gcgttggcaa cggggcggtc tttggcgacg attcccgaaa aggtcaggga
     5221 catgatggcg gaaacgggta tggatgccgt ggtaacgata aacggacagt ttgcgctgct
     5281 gcacggtaaa actgtgcgcg aagtaccgat ggataccggt ttgatgggca gggtttgtgc
     5341 gcatttggat ggcttgggca tggattatgc gtttgtcggc ggagagggga tcgccgtgtc
     5401 cgcgctgtcg gaatgcgtgt gccgcgcctt gcagcatatc gccagcgatt tttttgccga
     5461 taaggattat ttttcaagca aaccggtgta tcagatgctg gtgtttgcgg aggaaaacga
     5521 aatgccgctt tggtcggata ttgtggaacg ggaaggcttg aaaacggtgc gctggcacga
     5581 ggaagcggtc gatctgctgc ctgcgggaat gtcgaaaaca gacggcatca gaagcgtggt
     5641 tgaagcattg ggtttggaaa tggcagacgt gatggcgttc ggcgacggtt tgaacgatgt
     5701 ggaaatgctg tctgaagtcg ggttcggcgt ggcaatgggt aacggggaac aggcagcgaa
     5761 agaagcggcg aaatatgttt gccccggcgt tgatgaagac ggcgtgttga ggggcttgca
     5821 agatttgggt gtgatttgaa cgtataataa caaccctgcc ggtttcagac ggcagggtcg
     5881 gttttcagcc tttcatgcag ccttcgtttt gaagcagggt aaataagggc gcgccacttg
     5941 cgcggatatt cttgccgcct tgaaggtcgg taaattccaa aatggcggcg gcttcgacaa
     6001 tttccccgcc gagtttgcgg atcagttcca gcccggcaag cattgtgccg cccgtggcaa
     6061 ccaaatcatc gaccagcagg acgcgcgaac cgggtttgac ggcatcggtg tggatttcca
     6121 ccgcagcttc cccgtattcg agcgcgtagc tttgcgatac ggtttcaaaa ggcagcttgc
     6181 cttttttgcg gatggggacg aagccgacgt tgagctggta ggcgagtgcc gcgccgataa
     6241 tgaagccgcg cgcgtccaag ccggcaacga tgtcgatttt ctgatccata tagcggtaaa
     6301 ccagcaaatc gaccaaaagg cggaagtatt ccgcactttg caggacgggc gtgatgtcgt
     6361 ggaataagat gcctttttgc ggccagtttt cgattttgcg gattttgtcg gcaagcgcgc
     6421 cgacactcat agcttcggga tgaaccagca ttgcgtgttc caagtttgat gtttttaacg
     6481 ccatattgta gccgtcagcc tgccgcttgc caaatcaatc aaaacaatag cggcaaaata
     6541 tgggcatcgg caaatatgaa atagggtgtc cgttgtgata caatcggttg gaaaatatac
     6601 aggtaaaaaa tatgtccgct tatcgaaaag gcaacatctt tatcatttcg gccgcttccg
     6661 gcacgggcaa aaccacgctg gtgtcgcggc tgttggcaaa ccataacggt ttgcgcgttt
     6721 ccgtgtcgca tacgacgcgc ccgccgcgtg aaggcgaagc aaacggcgta cattatcact
     6781 ttgtttccaa agaagagttt gaatcgctta tcgcgcagga ggcttttttg gaatacgcag
     6841 acgtattcgg caactattac ggcacaagca cggagggcgt gaatgcgttg gcggcggcag
     6901 gctatgacgt gattttggaa atcgacgttc agggcgcggc gcaggttcgc aacgcgctgc
     6961 cggaagctgt cggtatcttt atcctgccgc cttctttcga cgtacttgcc gcgcgcctca
     7021 aagggcgcgg gacggacagt cgggaagtta tccaaaggag gctgtcgaag gcaaggcatg
     7081 aaatcgaaca gtccgtattg ttcgactttg tcgtggtcaa tgacgacctg gagaaggcgg
     7141 agggggattt gcttcatatt gtgaatgcct gccgtctgaa aaggtcgcgg caactgggat
     7201 ttattgcaga tttgttggaa aattcctaaa aaccgcgaaa atatccggtt ccccaattta
     7261 aatatttttg aaagaaagca aataatatgg cacgtattac caccgaagac tgtaccggaa
     7321 aaatttccaa ccattttgac ctgacattgg tagcggcccg tcgggcccgc cagcttgaaa
     7381 atggcaatac gccgcttgtg gacgatgtcc gcaataacaa accgaccgtt accgccttaa
     7441 gggaaatcgc agccgggcat atcggtacgg aactgttgac gcgcaataaa taaattctgc
     7501 cggaaacgca cgccggcaca ctctgccgcc gtgcagtccg acggtttgaa atgaaaaaac
     7561 acatacatcc cgaaagccgt ctgaaatgcc cgccccccaa ccttccgccc cttacgaccc
     7621 cctgaccgcc gaagcgcgta ccctgctttt ccataccgcc tcctacctca agcccgagga
     7681 acaggcggag cttgaaaaag ctgtcgccta tgcgtttcgc gcccacgacg ggcaaacccg
     7741 caaaagcggg gatccctaca tcacgcatcc gattgccgtt gcgacgcagc tcgccctttg
     7801 gcatatggac atacagggtc tttgtgcagg cgtgatgcac gacgtattgg aagatacggg
     7861 cgtgacaaaa ggggaaatgg cggcggtgtt cggcaatact gttgccgaga tggtggacgg
     7921 gctgtccaaa ctcgaaaaac tcaaatttga agatcatgcg gaacatcagg cggagagttt
     7981 ccgcaaactg attttggcga tgaccaaaga tgtgcgcgtc atcgttgtca aacttgccga
     8041 ccgcctgcac aatatgcgga cgctcggttc gatgcgcccg gacaaacgcc gccggattgc
     8101 aagggaaacc cttgaaatct acgcacagat tgccaaccgc ataggtctga ataatgcgta
     8161 tcgggagctt caggatttat cgttccaaaa cctgcatccc aaccgctacg agactttaaa
     8221 aaaagcgatg gacaagagcc ggaagaacag gcaggacgtt gtcggcaaag ttttgcgcgc
     8281 gttcggccag cggctggtag gcgcgaatat agaggccaaa atcaaaggca gggaaaaaaa
     8341 cctgtacggc atccatcaga aaatgatggc gaaaaagctg cgttttgccg aggttatgga
     8401 tatttacggt ttccgcgtca ttgtcaacag cattcccgcc tgttatgccg cactcggcac
     8461 attgcacacc ctctatcagc ccaagcccgg acggttcaaa gactatatcg ccattccgaa
     8521 aagcaacggt tatcaaagtt tgcatacgat tttggtcggc ccttacggct tgccgattga
     8581 agttcagata cgcacgcggg aaatggatgc cgtggcggaa ggcggtattg tcggccattg
     8641 gagctataaa tcagattcca aaacggtcga tcaggcagtg ctgcacacca atcaatggtt
     8701 gaaaaacatt ttagacttgc aggcaagcag cgccaatgcc attgagtttc tcgaacacgt
     8761 caaagtcgat ttgtttccga acgaaatcta catccttact ccgaaaggaa aaatcctaac
     8821 tttgcccaaa ggggcaacgc ctgtcgattt tgcttatgcg gtgcataccg acattgggca
     8881 taaaacagtt gccgcgcgtg tcaataatgt gatgatgccg ctgcgtacga agctcaaaac
     8941 cggtgattct gttgaaatta tcacatccga acacgccaaa cccaatcccg cgtggttgaa
     9001 tttcgccgtg tccggcaggg cgcgcagtgc catacgccaa tatattaaaa atcttaaccg
     9061 gcacgatgcg gtcgttttgg gagagagcct cttacaaaaa gccttgtcca gtttgctgcc
     9121 caaagatgtc ctgctttcag acggcatcaa agaaaaatac cttgccgatc tcaacgacaa
     9181 acagacttca tttgaggaag tgctgtacaa cgtcggtatg gggcataccc tgcctgttta
     9241 tgtcgccatg catatcgccg aattggcagg ggagcatttc ggcagcgagg tcaggctcag
     9301 ttccattaaa gttgatggtc aggaaagcgg gcatattcat tttgcagagt gttgccaccc
     9361 tgttcccggc gattccatcc gtttgctgtt ggttaaggga aaaggcatga ttatccatag
     9421 ggatacctgc ccgacgttgt tgaagtccga tcccgaacag cagctggatg cagactggga
     9481 aaatatgaac gggcagaact accgtgtcgg gcttcaagtc caatcggaag acagccacgg
     9541 cctgttggca ttaatggcgc aagcgatttc cgattccggt gcggacatcg agtcggtcga
     9601 aacaccgtct aaatcccagt cgggaacgga aggttttgtc gaattcaaat ttttattgaa
     9661 agttaagaat ttggatcagt tgaatcagat tattcaaaat ctgcacagca tcccatatat
     9721 ccgcaaagtc atcagaagtt gaagcaggta aaacgggttg cctgttaatc aaaaggcaac
     9781 ccgttttatg gtttatccgt tcaattgctt atttgttcaa atatacggca agcctttcaa
     9841 caatccgcat tgccgcttca tctttacttg tttcggggaa agacaactcc gcatcatcat
     9901 ctatgataac aatttggttg gttgtcttgc ccatcgcgat tgaaacatca ttggcaacta
     9961 tcaccggtat ttttttctta atacgttttt cccgcgcata tgccattaca ttctccgttt
    10021 cagcggcaaa accgatgcag aacggcgggt tcggcaatga ggcaatagaa gccaaagtat
    10081 cggggttctc atccaattcg atggataacg gtttggcatt tttatctttt ttgaattttt
    10141 gcgtactcct gtttttaacc ttatagtctg agacggcggc aacagaaata aaagcatctt
    10201 gtttttcaat caaacgatgt actgcgcgat gcatatcttc ggcactgacg gcttgaaccg
    10261 tatcggatat gccgaaaggc agcgtggttt gaagctgtcc gtgaatcagg ctgatttccg
    10321 caccggcggc acggcacgcc cgcgccaaag ctacgcccat ttttccgctg gaggtatttg
    10381 tgatgcccct gacagggtca atggcttcaa atgtcgcacc tgcggtaatc aagattttct
    10441 tgtcccttaa aattttcggt gtccacaagt ctggaagcaa atccagcaat tcggcaggtt
    10501 ccggcatcct tcccatacca ttttctctgc aagcctgttc gcccaagccc ggcatataga
    10561 cagtaatgcc gtctgaaacc agttgtgcga tattccgttg gttggcagga ttcagccaca
    10621 tttccacatt catcgcggga gcgatggcaa gagggcattt ccgtgcggct gccagattgg
    10681 tcagtaggtt atctgccacg ccgttacaga tttttgccac ggtattcata cttgccggcg
    10741 caatcagaaa aacatccgca ttccgggtca ggttgatatg ttccataccg tttgaaccgt
    10801 tgccgccgtg cgtgtcggtc agaacgggat tgccgctcaa agcctgaaaa gtcagcggag
    10861 aaacaaattc agttgccgag cggctcataa ccaccgtaac cgaatgcccc tgttttttca
    10921 gcagtcgcac caactcgcaa gacttatacg ccgcaatact gcccgttaca cctaaaagaa
    10981 tatgtttgcc cattttgaca atttctaatt acgaagaaaa aacagcagcc attctataac
    11041 aaagtacgga tacattagtt accgtttgga tac
//
LOCUS       DAXFHF010000065        10968 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXFHF010000065 DAXFHF010000000
VERSION     DAXFHF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.065"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..206)
                     /locus_tag="ACFIRM_001711"
     CDS             complement(<1..206)
                     /locus_tag="ACFIRM_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029293.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIP"
     gene            371..1295
                     /locus_tag="ACFIRM_001712"
                     /pseudo
     CDS             371..1295
                     /locus_tag="ACFIRM_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
     gene            complement(1374..2015)
                     /locus_tag="ACFIRM_001713"
     CDS             complement(1374..2015)
                     /locus_tag="ACFIRM_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3029294.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2070..2861)
                     /locus_tag="ACFIRM_001714"
     CDS             complement(2070..2861)
                     /locus_tag="ACFIRM_001714"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC3029295.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            3027..3102
                     /locus_tag="ACFIRM_001715"
     tRNA            3027..3102
                     /locus_tag="ACFIRM_001715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3060..3062,aa:Thr,seq:tgt)
     gene            3244..4035
                     /gene="panB"
                     /locus_tag="ACFIRM_001716"
     CDS             3244..4035
                     /gene="panB"
                     /locus_tag="ACFIRM_001716"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC3029296.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4158..4994
                     /gene="panC"
                     /locus_tag="ACFIRM_001717"
     CDS             4158..4994
                     /gene="panC"
                     /locus_tag="ACFIRM_001717"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC3029297.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5321..7159
                     /locus_tag="ACFIRM_001718"
     CDS             5321..7159
                     /locus_tag="ACFIRM_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3029298.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPGPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7299..7880
                     /locus_tag="ACFIRM_001719"
     CDS             7299..7880
                     /locus_tag="ACFIRM_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC3029299.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDKAGAVAVDAKGNVYQAESAE
                     ELGRQLIGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7890..8735
                     /gene="ispE"
                     /locus_tag="ACFIRM_001720"
     CDS             7890..8735
                     /gene="ispE"
                     /locus_tag="ACFIRM_001720"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC3029300.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8743..8818
                     /locus_tag="ACFIRM_001721"
     tRNA            8743..8818
                     /locus_tag="ACFIRM_001721"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8776..8778,aa:Gln,seq:ttg)
     gene            8831..8906
                     /locus_tag="ACFIRM_001722"
     tRNA            8831..8906
                     /locus_tag="ACFIRM_001722"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8864..8866,aa:Gln,seq:ttg)
     gene            9074..10057
                     /locus_tag="ACFIRM_001723"
     CDS             9074..10057
                     /locus_tag="ACFIRM_001723"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC3029301.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10124..10696
                     /locus_tag="ACFIRM_001724"
     CDS             10124..10696
                     /locus_tag="ACFIRM_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC3029302.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10822..>10968)
                     /locus_tag="ACFIRM_001725"
     CDS             complement(10822..>10968)
                     /locus_tag="ACFIRM_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFC3029303.1"
                     /translation="APVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTR
                     LTGQ"
BASE COUNT         2584 a         2588 c         3172 g         2624 t
ORIGIN      
        1 atggggataa acgaggttgc gccggtttga atgtaaaccg gtgcggcaag tgcggcgcgg
       61 ctgttttcgc tattcagata ggtaagggaa gtttccagtt tccattttcc cttgtcggtc
      121 attatgtctt caatcgtcaa gggcaggtcg gcataagcgg ataaaggcag gatggcgggc
      181 aaggcgggca aaaagatgcg tttcatattt tctcttattg atattaattc ttatttaaga
      241 atagtaatga atatccgata attctgtcta aataaatttt taaatcaaaa aaaacgatat
      301 atttgttttt atcaattatg ttttgatatg ccgtctgaaa agtttgtgaa aaacggttac
      361 aatccgccgt atgaaaaaac gcaataatcc tcttccgctg ttgaacggtg tcaaacccag
      421 ttatttggtg ctgccgcatg aaaagcagtt ttacgggctg ccgctgctgc attttctgtg
      481 catccgcttt ccttttgtgg gtgcggacga ttggcggagg cggttgaaca gcggttttgt
      541 ggtcggttcg gatggtgcgg cgttggatga acattctttg ttcgagccgg gtaagacgat
      601 gttttattac cgtgaaaccg gttgtgagag cgagccgcgt attccgtttg aagaaaagat
      661 tttgtttgtc gatgaacatt tgattgtggt ggacaagccg cattttctgc ccgttatccc
      721 cggcgggcgt tttttgcggg aaacgctgct cacgcgtctg cgcctgcgtc ccgaattgca
      781 gcatttgaat gttgaagaca ttacgccgat tcaccgcttg gacaaggata cggcgggtgt
      841 gatgctgctg tcgcacaatc ctgccacacg cggggcttat cagacgatgt ttcaaaacaa
      901 aacggtatgg aaaacgtatg aggcgcttgc gccgacaaag acggatttgc cgtatccgct
      961 cgatgtggtt tcgcgtttgg taaggggtga gaaatttttt acgacgcaag aggccgaagg
     1021 cgtgccgaat gcgcacacga cggtcgaatt gattgaaaac agggggggaa ttcagccttt
     1081 accgccttac gccgcatacc ggcaagaaac accagttacg cgtgcatatg atgggtttgg
     1141 gtatgccgct gttgaatgac gcgctctatc ccgtgccgtc tgaagcgggt agcgaggatt
     1201 atcggaaacc tttgaagctt ttggcaaaaa agattgcgtt tgcagatcct ttgtcgggtc
     1261 gggaaagggt gttttgcagc gggttttgcc tgtaatgaga gggaacgcaa acccgtatcg
     1321 gcagatgccg ccggatacgg gtttgatatt atcgatgctg tctgaaaggg gctttatccg
     1381 ttcaggcata cgggtgcgaa gcggaggatg gtgtcgcgca ggacgatgag tttgccgtga
     1441 aagaagtgcg tcgaaccggc gatggtaatg acgggcaaat cttgcggttc ggcccatttc
     1501 agtgcttttt cgatttcgac gacctcatct tccgcgccgt gtatcatcag cgttttggca
     1561 acgttgggaa cggcggacgg ctcggggcgg tcggtatagt ggcagactgc cgcaccgatg
     1621 agcagcaata aatcgggtat gcgcacttgt gcggcaaatg tggcgacata gccaccgaag
     1681 gaaaagccgg ataaggcaaa ttccggggct tcgggatgtt gggcgcgggc atagtcgatg
     1741 acggcgaggc agtcttgcgt ttcgccgcgt ccgtaatcat gtgtgccttc gctgccgcct
     1801 acgccgcgaa ggttgggaag gtagcagtgg aagccgagct tgctcaatgc tttggcggcg
     1861 gtttggatga ctttgttggt gttcgtccct ccttggaggg ggttgggatg attgatgacg
     1921 gcaacaccgc gtgccggtac ttgttcggac gggatatgga tggtttctaa aatgccggca
     1981 gggccgggta tgtgtatggt ttcgggtttc agcataatgg tttcctcttg attttccgca
     2041 tattccgatg cagccgcgaa gatggcggct taaaggtgga aatgcttgcc gttgttgggg
     2101 ttggacgctt tcaggccggc gagcatgcgg tggaagtcca atccgtatgc gttgctcagt
     2161 ttgtcggcac gttttttgag tttgtctatg ttttgctctt tggggctgct ttggaaggcc
     2221 attaccgcga cattgccgtg gctttcggca ggaagttcca atacgcgccc ttcaaaaacg
     2281 ctcaacaacc gttcgatgaa gcgttgatag cgtttgtcgc cgctccacca gttggtaacg
     2341 aatatgccgt ctgaagagag tgcgttgcgg cagtctctga aaaacggttc ttcaaccagc
     2401 gtatcgatga tttgttcgcc gtcgaatccg tccaccaaga tgatatcggt gttgtggcgg
     2461 aagactttga tgtattccgc accgtctgct tcgataattt caaattttcc gccctcgaaa
     2521 ggcaactcaa acaggttgcg ggcaatggcg atgacctgag gattgatgtc cacggcggtt
     2581 tggcgcgtgt cgggcaggta ggtgtctgtc cagcgtgcaa acgaaccgcc gcccaagccg
     2641 atttgggtga tatgttgcgg caggcgttcc gcaaacagca gccagcccat catcgcgcgg
     2701 ctgtaagaga gcaccagctc ggacgggcgg tcgaggttca tcgagctttg aacggtgtcg
     2761 ctgcccaagt gaagcgaacg gatattgcct tcttcggaaa taccgacttc gggaaagccg
     2821 gattttgcag gacgcaggcg gcggtaggga tgtcttgcca tcggtgtgaa cggtcggttt
     2881 gaaaggcgga tattttatca gaatgccgat gcttgttctg tttcaaatta attttgtttt
     2941 aaataagata cttagtgtat ttttatgagg gggtcgaaca aatttcttgc caaagtgtga
     3001 agttgcctgc ataattcgcc ttctttgccg gtatagctca gttggtagag cacctgactt
     3061 gtaatcaggg ggtcccgagt tcgactcttg gtgccggcac cagatttgac agcccgattg
     3121 tgtaaaacag tcgggctgtt ttgcatttgc ggcacggtcg gtggcagggc tgcgggcatt
     3181 tcactataat agcccgtttc aaactgaaaa ggccgtctga aaagggcggg gtaacaatat
     3241 ctgatgatta ctgtgaacac actgcaaaaa atgaaggcgg ccggcgagaa aatcgttatg
     3301 ctgaccgctt acgaatccag ttttgccgcg ctgatggacg atgccggcgt ggatgtgctg
     3361 ttggtcgggg attctttggg aatggcggtt caggggcgac agtcgacgct gccggtcagc
     3421 ctgcgcgata tgtgctatca caccgaatgt gtagcacgcg gtgcaaaaaa tgcgatgatt
     3481 gtcagcgatt tgccgtttgg tgcatatcag cagagtaagg agcaggcgtt tgccgccgcc
     3541 gccgaactga tggctgccgg cgcgcatatg gtcaaactcg aaggcggcgt gtggatggcg
     3601 gaaacgactg aatttctgca aatgcgcgga attccggtct gtgcccacat cggtctgacc
     3661 ccgcaatccg tgtttgcctt cggcggatat aaagttcagg ggcgcggcgg caaggcgcag
     3721 gcgttgctta acgatgccaa ggcgcatgat gaagccggag ctgccgtcgt actgatggaa
     3781 tgcgtgccgg cggaactggc aaaaaaggta actgaaactg tttcctgtcc gaccatcggc
     3841 atcggggcgg gcgcggattg cgacggtcag gttttggtga tgcacgatat gctcggtatt
     3901 ttcccgggca aaacggcgaa attcgtcaaa aactttatgc gcgggcaaag cagcattcag
     3961 gctgcggttc gggcgtatgt tgccgaagtc aaagccaaaa ccttccctgc tgcggaacat
     4021 atttttgcag attgaacaag gtctgctgcc gatgccgtct gaaagccgtt tcagacggca
     4081 ttttgttttg ccgtgcgcgg cgcgtataat cggcgcgttt tgtcgggcag gaagcccgaa
     4141 ggataaggat taccgtaatg caaatcatac ataccattcg agaactgcgc acgtggcgtg
     4201 aaaatatagg aaaggtggca tttgtgccga ccatgggcaa tctgcatgaa gggcatcttg
     4261 cccttgttcg cgaagccaga aaacgcgccg acaacgttgt tgtcagcata ttcgtcaacc
     4321 gcttgcaatt cggtcagggc gaggatttcg acaaatatcc gcgtactttg caacaggatg
     4381 cggacaaact tgccgccgaa ggcgttgccg ttgttttcgc gcccgatgag aaagaactct
     4441 atccgaacgt ggaacagcgt tacaacgtcg aaccgcccca tttgcaaaac gaattgtgcg
     4501 gcaaattccg ccccggccat tttcgcggcg tggcgacggt ggtgtccaag ctgttcaata
     4561 tcgttttgcc ggatgttgcc tgtttcggca aaaaagatta ccagcagctt gccgtgatta
     4621 aaggtttgac cgaggatttg aatttcgata tcgaaattgt ccccgtcgat acggggcggg
     4681 cggcggacgg tttggcactc tccagccgca accggtactt gagcgttggg gaacgcgccg
     4741 aagcaccgcg cctgtaccgc gagctgcagg ctgttgccga atcgctgaag cagggcggtt
     4801 tggattatgc gggtttggag cggcaggctg ccgaccattt gaccgctgcg ggctggctgg
     4861 tcgattatgt ggaaatccgc cgcgccgata cgctcgaaat ggcgcgggcg ggagataaga
     4921 aactggtggt cttggccgcc gcccgtctgg ggacgacgcg tctgattgac aatgtggaag
     4981 tcggcctgcc ttagcccgcc ggagccggga atgccgcctg aagcggattt gcgtttcaga
     5041 cggcattttg ccgtcggctg tgcttcggaa taatgtcgga aaatagacgg aatgaaataa
     5101 atttcttttt gttcaacaaa ttaaactgtc aaaccaaata ataaggcaag gattgtccgg
     5161 catcgcatac aggcaaaccg acgcaatctc aaaacacagc cctgccttcg ggcgggtttt
     5221 gcaaatctac aaaccgtttg gcttgtgata aagtgacgcc ttgtctagat actcaatatg
     5281 ccgtctgaag gttcggacgg atgtcggata aaggatgatt atgttacccg cccgtttcac
     5341 tattttatct gtcctcgcag cagccctgct tgccggacag gcgtatgctg ccggcgcggc
     5401 ggatgtggag ctgccgaagg aagtcggaaa ggttttaagg aaacatcggc gttacagcga
     5461 ggaagaaatc aaaaacgaac gcgcacggct tgcggcagtg ggcgaacggg tcaacagggt
     5521 gtttacgctg ttgggcggtg aaacggcttt gcagaaaggg caggcgggaa cggctctggc
     5581 aacctatatg ctgatgttgg aacgcacaaa atcccccgaa gtcgccgaac gcgccttgga
     5641 aatggccgtg tcgctgaacg cgtttgaaca ggcggaaatg atttatcaga aatggcggca
     5701 gatcgagcct ataccgggtg aggcgcaaaa acgggcgggg tggctgcgga acgtattgag
     5761 ggaaggggga aatcagcatc tggacgggtt ggaagaggtg ctggcgcaat cggacgatgt
     5821 gcaaaaacgc aggatatttt tgctgctggt gcaagccgcc gtgcagcagg gtggggtggc
     5881 tcaaaaagca tcgaaagcgg ttcgccgtgc ggcgttgaaa tatgaacatc tgcccgaagc
     5941 ggcggttgcc gatgcggtgt tcggcgtaca gggacgcgaa aaggaaaagg caatcgaagc
     6001 tttgcagcgt ttggcgaagc tcgatacgga aatattgccc cccactttaa tgacgttgcg
     6061 tctgactgca cgcaaatatc ccgaaatact cgacggcttt ttcgagcaga cagacaccca
     6121 aaacctttcg gccgtctggc aggaaatgga aattatgaat ctggtttccc tgcgtaagcc
     6181 ggatgatgcc tatgcgcgtt tgaacgtgct gttggaacac aacccgaatg caaacctgta
     6241 tattcaggcg gcgatattgg cggcaaaccg aaaagaaggt gcgtccgtta tcgacggcta
     6301 cgccgaaaag gcatacggca gggggacggg ggaacagcgg ggcagggcgg caatgacggc
     6361 ggcgatgata tatgccgacc gcagggatta cgccaaagtc aggcagtggt tgaaaaaagt
     6421 gtccgcgccg gaatacctgt tcgacaaagg cgtgctggcg gctgcggcgg ctgccgaatt
     6481 ggacggcggc cgggcggctt tgcggcagat cggcagggta cggaaacttc ccgaacagca
     6541 ggggcggtat tttacggcag acaatttgtc caaaatacag atgctcgccc tgtcgaagct
     6601 gcccgacaaa cgggaagccc tgatcgggct gaacaacatc atcgccaaac tttcggcggc
     6661 gggaagcacg gaacctttgg cggaagcatt ggcacagcgt tccattattt acgaacagtt
     6721 cggcaaacgg ggaaaaatga ttgccgacct tgaaaccgcg ctcaaactta cgcccgataa
     6781 tgcacaaatt atgaataatc tgggctacag cctgctttcc gattccaaac gtttggacga
     6841 gggtttcgcc ctgcttcaga cggcatacca aatcaacccg gacgataccg ccgttaacga
     6901 cagcataggc tgggcgtatt acctgaaagg cgacgcggaa agcgcgctgc cgtatttgag
     6961 gtattcgttt gaaaacgacc ccggacccga agttgccgcc catttgggcg aagtgttgtg
     7021 ggcattgggc gaacgcgatc aggcggttga cgtatggacg caggcggcac accttacggg
     7081 agacaagaaa atatggcggg agacgctcaa acgctacgga atcgccttgc ccgagccttc
     7141 ccgaaaaccc cggaaataat gcaggcccat cctttcagac ggcataaggt ttgccgggaa
     7201 gccggggcat tcgggcaaac ggcacgcagt tcgcacgcgt tttgcacggc acgccgaacc
     7261 cgccggccgg caggatgaca cccgttaagg aaactctgat gaaacacacc gtatccgcat
     7321 cggtcatcct gcttttgacc gcttgcgcgc aattacctca aaataacgaa aacctgtggc
     7381 agccgtccga acacatcagc agttttgcag cggaagggcg gttggcagtc aaagcggaag
     7441 ggaaaggttc gtatgcaaat ttcgattgga cataccaacc gcccgtggaa accatcaata
     7501 tcaacacccc tttgggcggc acgctcggcc agttgtgcca agacaaagcc ggtgcggtgg
     7561 cagtggatgc caaaggaaat gtttatcagg cggaaagtgc ggaagagttg ggcaggcagc
     7621 ttattggttt caagctgcca atccaatatc tgcatatctg ggcagaaggc aggcgtgtgg
     7681 cgggcgcgcc ttaccgcatc cgttcagacg gcatattgga acaatacggt tggacaatcg
     7741 gcagaaccgc cgacagtggg gggcaagtcc gaacgttgca actgaataac ggaaatttga
     7801 acatcaggct ggttttcacc gaaattggaa tgccgtctga aaccgaaacc ccggaacgct
     7861 gtgcggcgcg cacgagataa ggcggacaga tgaatattgc ggacggacgg caggcgtttc
     7921 ccgcacctgc aaaactgaat ctcgatttga ggattaccgg caggcgggaa gacggttatc
     7981 acaatatcga aagcatattc tgcctgatag atttgcagga taccgtatat ttgaaaccga
     8041 gggacgacgg caaaatcatc ctgcacaatc ctgtcggcgg aataccgcag gaagccgatt
     8101 tgagctaccg tgccgcatcg ttgctgcaaa aatatgcgcg caaccttgcc ggcgtggaaa
     8161 tatggctgga caaaaaaatc ccgaccggcg ccggcttggg aggggggagt tccgatgccg
     8221 caacggtttt gctggtgttg aaccgttggt ggcagtgtgg tttgacgcag tggcagctca
     8281 ttgatttggg tgcggctttg ggtgcggacg taccgttttt tattttcgga aaaaatgctt
     8341 ttgcaagcgg gataggtgaa aaactgatag gaatggatat tccgaaacag tggtatgtta
     8401 ttgtcaaacc tcccgtccac gtttccaccg caaaaatttt cacatacgaa ggcttgacac
     8461 gggattctgc ctcaagcata atgccgactt tccaaaacct gcaaccgttc agaaacgata
     8521 tgcaggcagt ggtatttaaa gaataccctg aagtttggaa agcttattcc gagttgtcga
     8581 aatacggttc tgcaatgatg accggctcag gagcctgtat atttgcagca tttcaagcta
     8641 ggaatagcgc atacaatata taccgacaag tttcaggttt gtacgaggca tatttggcag
     8701 agggtctttc aaaacatcct ttgttgtccg tataaacatt gttggggagt cgtcaagcgg
     8761 ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc cccagccaag
     8821 ccaaacaagt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
     8881 aggttcgaat ccttcctccc cagccagaca aaagcgtgta agcctgctta cacgcttttt
     8941 atttcataga aataaaaata ttgaaacgcc tttgtttgtg tcggatgttg caggtataat
     9001 gccgggcttg gtacaagcgg agggaagcat tgtgttttct gagcggaagt taaacataaa
     9061 atcaggtgag aatatggctg cgtacgacag tttgatggta tttacaggca atgccaatcc
     9121 cgaattggca caacgtgttg tcaggcattt ggacatttct ttgggcaatg cttccgtatc
     9181 caagttttca gacggcgaag ttgccgtcga actgttggaa aacgtacgcg ggcgcgacgt
     9241 tttcatcctt cagccgacct gtgcgccgac caatgacaac ctgatggaaa tcctgacgat
     9301 ggcggatgca ctgaagcgtg cttcggcagg tcgtattacc acagccattc cgtatttcgg
     9361 ctatgcgcgc caagaccgcc gtccgcgttc cgtccgcgtt ccgatttctg ccaaactggt
     9421 ggcaaatatg ctgtattcgg cagggatcga ccgtgttttg actgtcgatt tgcatgccga
     9481 ccagattcaa ggtttcttcg atattccggt ggacaatatt tatgccaccc cgattttgtt
     9541 gaacgacatc aagcagcagc ggattgaaaa tctgaccgtc gtcagcccgg acatcggcgg
     9601 tgtcgtccgc gcccgcgccg ttgcaaaatc cctgaatgcc gacttggcaa tcatcgataa
     9661 acgccgcccg aaagccaatg tggcggaagt catgaacatc atcggcgata ttcaaggcaa
     9721 aacctgtctg attgtggacg atatgattga cactgcaaat acgctgtgca aagccgccgt
     9781 cgccttgaaa gagcgcggtg cagaacgtgt cttggcttat gccagccacg ccgtattctc
     9841 cggagaggcg gtcagccgta tcgcctcatc cgaaatcgac caggtggtcg taaccgatac
     9901 cattcctttg tctgaagcgg ctaaaaactg cgaccgtatc cgtcaggtaa cgattgcagg
     9961 tttgctggcc gaaaccgtcc gccgtatcag caacgaagaa tccgtctcat atctttttaa
    10021 tgaagaagtg atgacaggca gcatgttgct gccgtaaccc cgaagccgtc ttaagctggt
    10081 cgcggccgat gacggcagtt ttatttaact tggagtattt aacatgactt atgaaattca
    10141 agcctctgtt cgtgaagcac aaggcactgg tgcgagccgc cgcctgcgtc gcgaaggtca
    10201 aatccccggt attttgtacg gtgaaggtca agagcctgtt gccattgctg tggatcacaa
    10261 aaccgtattt tacgcattgg aaaaagaatc tttccatact gcgttgatta aattgtctct
    10321 gaacggcgaa accaaagacg ttatcgtccg cgatttccaa atgcacccgt tccgccgcga
    10381 agtccaacat atcgacttcc aagccgttaa agcagaccaa cccgtacgca tccgcgttcc
    10441 cctgcacatc gttaacgctg aaaattctca agccgttaaa ttgcaaggcg gtcgcgtatc
    10501 tctgctgaac acggctgttg aagtgcttgc gttgcctgcc aatatccctg ctttcttgga
    10561 tttggattgt gccgaagtgg ttgccggcga cattttgcac ttgtcagaca tcaaactgcc
    10621 tgaaggtgta gaaagcgttt ccctgaaacg taacgaaaat ctggctgttg ctaccgttac
    10681 cggtaaaaag cgctaactta cttccatcag cagggcggcg cgtatgcaat acgtaccgcc
    10741 ctgttgtttt atgccgtctg aaccgtgttt cagacggcat ttctttattt gttggaaaag
    10801 cgggatattt gaaacggcag attactgccc tgtcagacgc gtccaaagcc tttgccaccg
    10861 gcttcttttt tctacatttt ccagtgcgac gatttctttt tcggcaatgg tatgtccgtt
    10921 ttgcctgatt ttgatttttc ctaaaatctg cccttttttt accggggc
//
LOCUS       DAXFHF010000066        10513 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXFHF010000066 DAXFHF010000000
VERSION     DAXFHF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.066"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(71..1309)
                     /locus_tag="ACFIRM_001726"
                     /pseudo
     CDS             complement(71..1309)
                     /locus_tag="ACFIRM_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1489..2862)
                     /locus_tag="ACFIRM_001727"
     CDS             complement(1489..2862)
                     /locus_tag="ACFIRM_001727"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3029304.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3076..4080
                     /gene="dusB"
                     /locus_tag="ACFIRM_001728"
     CDS             3076..4080
                     /gene="dusB"
                     /locus_tag="ACFIRM_001728"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC3029305.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKCRLNIPAWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4093..4242
                     /locus_tag="ACFIRM_001729"
     CDS             4093..4242
                     /locus_tag="ACFIRM_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029306.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4269..4508
                     /locus_tag="ACFIRM_001730"
     CDS             4269..4508
                     /locus_tag="ACFIRM_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC3029307.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4511..5047
                     /gene="ruvC"
                     /locus_tag="ACFIRM_001731"
     CDS             4511..5047
                     /gene="ruvC"
                     /locus_tag="ACFIRM_001731"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC3029308.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5098..5967
                     /locus_tag="ACFIRM_001732"
     CDS             5098..5967
                     /locus_tag="ACFIRM_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC3029309.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            6871..7665
                     /locus_tag="ACFIRM_001733"
     CDS             6871..7665
                     /locus_tag="ACFIRM_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC3029310.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDTGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            7890..10493
                     /gene="pepN"
                     /locus_tag="ACFIRM_001734"
     CDS             7890..10493
                     /gene="pepN"
                     /locus_tag="ACFIRM_001734"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC3029311.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGDPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLMT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPETASWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         2408 a         2749 c         2985 g         2371 t
ORIGIN      
        1 tgtgtgaatg atgaaataaa aatgccgtct gaacccgatt ggattcagac ggcattttgt
       61 atggcaggat ttattcgctt tccaactgac cgacccattc tgacaaggca gtcaggcgtt
      121 gttcggattt cgccacgaac gggttttcgg tatcccacgc gtagcctgcc aaaatcgaag
      181 aaagcatacg gctgatttcc gggctgcggt cgggcgcgta cacgacgttt tggaagcgga
      241 aatcatacca gccgtccaca taggtgcgga acgcgtctac gccgatcatc aggggttcgg
      301 caaattcggt ttgccaatcg gcggcttcgc ctttgagttg ttttgtcagc agatcggcag
      361 caagttcggc ggagtgcagc gcgatggtta cgcccgacga gaacacgggg tcgaggaact
      421 cggcggcatt gcccaacagc gcgaaatgcc tgccgtgcag tgatttgacg ttggcggaat
      481 agccttggat ggagcggaac ggaaaatcgt tttcccaaac ggctttgtcc aaaatttcgc
      541 tcaacatcgg gcattcgtaa acaaattttt tcaacaccgt ttccgattcg ccggcaagtt
      601 tgtcgggtgt gccgaccacg ccgacggaac agcggttgtc gccgaaggga atcagccaaa
      661 tccacacgtc gcggttgttg cggatgggtg gtaatcagga ttttgttgcg gtcgaatttc
      721 gggtgggtaa tgttgtcgtc gatgtgcgtg aaatgcgttt ggcgcggcgg caggtgcgag
      781 ggcgtttcca agtttagcag gcgcggcagc acgcgtccgt agccgcttgc gtccaagacg
      841 aatttcgcgg tcagttcata gctctcgccg gtgtcggttt cgatgttcaa gcgggcaaaa
      901 tcgccgctgt tgtcgaacgc ggttacgccg tgcccgaaac gtacttcaac gccttgtttg
      961 gcggcttctt caatcaggat tttgtcgaac acggcgcggc ggacttggta aaccgtgccg
     1021 gggccgtctg aaaatttatc ggtgaaatca aactcggtat agcggctgcc ccacgaaaac
     1081 gccgcaccgt ttttcaactg aaagccgggc ccggtgcgaa cggcatcggc aaaaccggct
     1141 tcttccagca tttccataca gtgcggcagc aggctttcgc cgatgacgaa gcgcggaaag
     1201 tgctgttttc caacacgcag acttgataac cttttttgcg gagcagggcg gatgcggccg
     1261 aacctgccgg acctgcgccg attacggcga catcgaattg ggtgggcatt gggaaatcct
     1321 gatggtttga gtggaaaagg aaatgatatt gtacaggcag caaatgccgt ctgaaactgt
     1381 ttgcggaaga aatttatccg gtgcgggcgg ttgctcccaa tgcgtcaatc cggtttgaaa
     1441 aatgccgtct gaaacgggaa atgttcagac ggcatttgat tttcagggtt attttacgcc
     1501 gtaacgcggt tggagcaggg cgcacgtccg gtgtccccgg cggcttcggc ggctgcgccc
     1561 ggcaattttt ccgcaggccg cgcccgcatc gtttgccgtg gtttccggcc gggtattttt
     1621 ttcgcgctga gccctggcgg attcgtagcg gttgcgttgt ctcggttcgc ttgtttcggt
     1681 tttttccggt tttgagcctt cctgttccca ccatcggggc tcgaagcctt cgatgcgttc
     1741 gatgagcagc ttgttgccgg tcagttcttt gatggcctca aacattttct gttcggattc
     1801 gtccatcagg gaaatcgcca cgccgtccgc accggcgcgc cccgtgcgtc cgatgcggtg
     1861 gatgtagtct tcgggctggg cgggcatttc gtaattgatg acgaagggca gttcggcgat
     1921 gtccagccca cgcgcggcga tatcggtggc gacgaggacg cgcaggctgc cgtctttgaa
     1981 ggcgttgagt gtttcgagcc tgctttgttg ggaacggtcg ccgtgtatcg cctgtgcgga
     2041 caggttgcgg cgcaccagtt cgcgcgttac gcggtcgacg ctttgtttgg ttttgcaaaa
     2101 gacgataact tggttcatat gcagatcgac aatcagccgt tcgagcaggt tgcgcttttg
     2161 gaaggtatcg acggcgatga tgtgttgttc gacgttggcg ttggtggtgt tttgggcggc
     2221 aacctcgacg gtttcgggcg cgttcatgaa gtcttgcgcc agtttgcgta tcggtgcgga
     2281 gaaggtggcg gaaaagagca gggtttggcg ttggcggggc agcatctgca tgattttgcg
     2341 gatgtcgtcg ataaacccca tatccaacat gcggtctgct tcgtccagaa cgacgatttc
     2401 ggctttgttt aaactgatgt ttttctgttt cacatggtcg agcagccgtc cgacggtggc
     2461 gacgactatt tcgcagccgg cacgcaggtc ggcggtttgt ttgtccatgt tgacaccgcc
     2521 gaagaggacg gtatgccgca gcggcaggtt tttaatgtag ccctgcacgt tttggtcgat
     2581 ttggtcggca agttcgcgcg tcggggtcaa cacgagcata cgcacggggt gcattgcagg
     2641 cgaggtgctg gcggtggcgt aacgtttgag gcgttccaga ctgggcagca taaaggcagc
     2701 ggttttgcct gtgccggttt gcgcggcggc aagcaggtcg tgtccggcca gtgctttggg
     2761 aatggccgcg gcttggatgg gcgtcgggtt ttcgtaacct tgcgcggtca gtgcggaaac
     2821 cagttccgta cccaaaccta aagaggaaaa tggattactc atgattgtag tctttctttc
     2881 agaccttatg ccgtctgaag cgggaaaccg ataggacggg gaaataaact gcccgcctgt
     2941 gaacggcggg gacgggaatt tgtgtgcgga ttatgccata aaacggtgcc cgccccaatc
     3001 gcgggcgcgt ccggagattg gaaatcctgc ttaaaaaatg tacaatggcg cacttttttg
     3061 aaacgcgggc ccattatgca catcggcggt tattttatcg acaaccccat cgcacttgcg
     3121 ccgatggcgg gcattaccga caaacccttc cgccgcctct gtcgggcgtt tggcgcaggt
     3181 tgggcggtgt gcgaaatgct ggccagcgat ccgacgctca ggaataccgg aaaaaccctg
     3241 caccgcagtg attttgccga tgaaggcggc atcgttgccg tgcagattgc cggcagcgac
     3301 cccgaacaga tggcggatgc ggcgcgttac aacgtcggac tcggggcgca ggtcatcgac
     3361 atcaatatgg gctgccccgc caagaaagtg tgcaacgtcc aagccggtag cgcgctgatg
     3421 caggacgagc cgctggttgc cgccattttg gaggcggtgg tcaaggcggc gggcgtaccc
     3481 gttaccctca aaacccgttt gggttggcac gacgaccatc aaaacctgcc cgccatcgcc
     3541 aaaatcgccg aagattgcgg cattgccgcc cttgccgtcc acgggcgcgc gcgcacgcaa
     3601 atgtacaaag gcgaggcgcg ttacgaactc atcgccgaga ccaaatgccg tctgaacatc
     3661 ccggcctggg tcaacggcga catcacttcg ccgcaaaaag ccgccgccgt cctcaaacaa
     3721 accgccgccg acggcatcat gatagggcgc ggcgcgcaag gcaggccgtg gtttttccgc
     3781 gatttgaagc attatgccga acacggcgtt ttaccgcctg ccttgagttt ggcagaatgc
     3841 agagccgcca ttttgaacca catccgcgcc atgcacgcgt tttatggtga gaccgtcggt
     3901 gtgcgtatcg cacgcaaaca cataggctgg tacatcggcg aaatgcccga cggcgaacag
     3961 gcgcggcgtg aaatcaaccg cttggacaat gcggcggcgc aatacgacac acttgccggt
     4021 tatcttgaaa ggcttgccgg aaaaaccgac cgttgggcgt gcggctatcg ggaagggtag
     4081 ggcagtattg ccatgccgcc gttcgggttc ggacggcatc tgtctgcatg gttcggaggt
     4141 cgggcggaat cccggccggc gaatgtcgaa aacaataaat gccgtctgaa aacgagtgga
     4201 acgggtttcg ccaaaaggct ttcagacggc attccgtctt aaaaacattt caatcaaaag
     4261 gatatgcgat gaaccctgcg actgcggaca ttgcgcaatg tatcgagcag aacttgaacc
     4321 aatatttcaa agacctgaac ggcaccgaac cttgcggcgt gtacgatatg gtactgcatc
     4381 aggtggaaaa gccgctgctg gtgtgcgtga tggagcaatg cggcggcaac cagtccaaag
     4441 cctccgtgat gctgggactg aaccgcaata ccttgcgtaa gaaactgatt caacacggtt
     4501 tgctgtgaat atgtcggcaa ccgtccgtat cttgggcatc gacccgggca gccgcgtaac
     4561 gggtttcggc atcatcgatg tcagggggcg cgatcatttt tacgtcgcct ccggctgcat
     4621 caaaacgcct gccgatgagc ctctggcaga caggattgcc gtgattgtgc ggcatatcgg
     4681 cgaagtcgtt gccgtttaca agccgcaaca ggcggcggtg gaacaggtgt tcgtcaacgt
     4741 caatccggca tcgacgctga tgctcggtca ggcgcgcgga gcggcattgg cggctttggt
     4801 cagccataag ctgcccgttt cggaatacac ggccttgcag gtcaaacagg cggtggtcgg
     4861 caaaggcaag gcggcgaaag aacaggtgca gcatatggtg gtgcaaatgc tgggactttc
     4921 gggaacgccg caggcggatg cggcggacgg tcttgccgtc gcgctgaccc acgccttacg
     4981 caaccacggg cttgccgcca aactcaatcc ttcggggatg caggtcaagc gcggaaggtt
     5041 tcaatagttt cagacggcat ttgtattttg ccgcctgaaa agaaaatgtg taccgagatg
     5101 aaatttatat tttttgtact gtatgttttg cagtttctgc cgtttgcgct gctgcacaag
     5161 attgccggcc tgatcggttc gcttgcctac cttctggtca aaccgcgccg ccgtatcggc
     5221 gaaatcaatt tggcaaaatg ttttcccgaa tgggacgaag aaaagcgtaa aaccgtgttg
     5281 aaacagcatt tcaaacacat ggcaaaactg atgctcgaat acggcttata ttggtacgcg
     5341 cctgccaaat gcctgaaatc gctggtgcgc taccgcaata agcattattt ggacgacgcg
     5401 ctggcggcgg gggaaaaagt catcatcctg tacccgcact ttaccgcgtt cgagatggcg
     5461 gtgtacgcgc ttaatcagga tgtcccgctg atcagtatgt attcccacca aaaaaacaag
     5521 atattggacg aacagatttt gaaaggccgc aaccgctatc acaacgtctt ccttatcggg
     5581 cgcaccgaag ggctgcgcgc cctcgtcaaa cagttccgca aaagcagtgc gccgttcctg
     5641 tatctgcccg atcaggattt cggacgcaac aattcggttt ttgtggattt tttcggcatt
     5701 cagacggcaa cgattaccgg cttgagccgc attgccgcgc ttgcaaatgc aaaagtgata
     5761 cccgccattc ccgtccgcga ggcggacaat acggttacat tgcaattcta tcccgcttgg
     5821 aaatcctttc cgagtgaaga cgcgcaagcc gacgcgcaac gtatgaaccg ctttatcgaa
     5881 gaacgcgtgc gcgaacatcc ggaacaatat ttctggctgc acaagcgttt caaaacccgt
     5941 ccggaaggca gccccgattt ttactgacta cataaaatta caaaacaaat caggcgtttc
     6001 agatcaaaaa ccccgattgt ttttgggaat ttgaaacccg ggttgtacaa acaggatttg
     6061 ccggacggtt ttaacggttc agttgtttgt aaaaacaatg cttttttaaa attgacaaaa
     6121 aacgaaatcg gttttaaagg cttattccga gaacaaaggg gagtggatgc cgaaaacccg
     6181 gttaatatat tatagtggat taacaaaaac caatacggcg ttgccccgcc ttagctcaaa
     6241 gagaacgatt ccctaaggtg ctgaagcacc aagcgaatcg gttccgtact atttgtactg
     6301 tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctataaaatt aaatttgttt
     6361 aaaaacataa agttgtaaac aagtatctca tataagcctt tttcattaaa cagatagtca
     6421 gatattttgt gctaaaaatt tatataatat ttaaattaat atcaagttat aaaaaatata
     6481 tggaatttta ttttgtttat ttataatttt aagcaataat ctaaatcagg catttttatt
     6541 cccctgtttt aaaaaatatt tggcaaggtg tgaaaaaagg cgtacattcc gctacacaga
     6601 attacagata cagcggagca atgccgtctg aaaggatttt ccggtcagtc ttgcggttgg
     6661 tcggggtttc atcggatacg gtgaaacgaa agtttgccgg cgcagggttg agctacgcgg
     6721 gtaaagccgc aggcgaaagc ctgtattgtt tgtgaagcgt aaatctctga tttgaggtat
     6781 tggggcaatc ctgtgggggt tgcctctttt tttatccgcc ttttaatgac acaataggcg
     6841 caagtctgtt tttgaatgca aggtactgcc atgaatacga ttttggcttt cgatattgaa
     6901 accgtacccg atgtgcaggg tatccgtacg ttgtatgact tgccgtcttc cctgccggat
     6961 gacgaagtgg tgctgttcgc gcagcagaaa cgccgcgcac agaccggcgg cgattttatg
     7021 cagcaccacc tccatcaggt tgtggcggtt tcctgctgta tgcgctgggg gcaggacaag
     7081 gttcatgtcg gcaccatcgg cgagatggac gatggcgaag aagtcgtgat tgccaagttt
     7141 ttcgaattgg ttgaaaaaca tacgccgcaa ctggtcagct ggaacggcgg cggtttcgac
     7201 ctgcccgtac tgcattaccg ctccctgata tacggcatca acgccgcgcg ctattgggat
     7261 acgggcgacg gcgatttcgg cgacagccgc gatttcaagt ggaacaacta catcagccgt
     7321 tatcaccaac gccactgcga tttgatggat ttgctcgcgc tttaccagcc gagggcgaat
     7381 gtgccgctgg acgatatggc gaaactgtgc ggctttccgg gcaagctggg tatggacggc
     7441 agcaaggtgt gggaggcgtt ccacgcgggc aggctgaagg aaatccgcaa ttattgcgaa
     7501 accgatgccg tgaatacgta tttgatgtat ctgcgtttct gtctggtcag cggcagattc
     7561 gacgcggacg aatacgaaat ggaaatcaag cggatcagaa actatctctc cgcccaaaca
     7621 gaagacaaac cgcattgggc agaatttgtc caagcgtgga aatagaaacc tgctgtccga
     7681 aggcggtacg cttgtgccgc cgtttcggtt tcagacggcg tatggtggat taaattcaaa
     7741 ccggtacagc cttgcctctc cttgccttac tatctgtact gtctgcggct tcgtcgcctt
     7801 gtcctgattt ttgttaatcc gctttaaaag ctgtatccgt gccgtctgaa gccttttcca
     7861 gtcaaaccaa acaatattga aagaaaaaaa tgagcaaaac cgtccgttat ctgaaagatt
     7921 accaaacgcc tgcctaccgc attcttgaaa ccgaactgca tttcgacatt gccgaaccgc
     7981 aaaccgtcgt gaagtcgcgt ttgacggtcg agccgcagag ggcgggcgat ccgctggtgt
     8041 tggacggttc ggcaaaactt ttgtccgtca aaatcaacgg cgcggcggcg gattatgtgt
     8101 tggaaggcga gacgctgacg attgcagacg taccgtccga acgcttcacc gtcgaagtgg
     8161 aaaccgaaat cctgccggcg gaaaacaaat cgctgatggg gctgtatgct tccggcggca
     8221 atctgtttac ccagtgcgag ccggagggct tccgcaaaat cacgttctac atcgaccgtc
     8281 cggatgtgat gtccaagttc acgaccacca tcgtcgcgga caaaaaacgc tatcccgttt
     8341 tgctttccaa cggcaacaaa atcgacggcg gcgagttttc agacggccgc cattgggtga
     8401 aatgggaaga cccgtttgcc aaaccgagtt atctgtttgc tttggtcgcg ggcgatttgg
     8461 cggtaacgga agaccgtttc accaccatga gcggcagaaa cgtcaaaatc gagttttaca
     8521 ccaccgaagc ggacaaaccc aaggtcggct ttgccgtgga atcgttgaaa aacgcgatga
     8581 agtgggacga aacgcgcttc gggttggaat atgacttgga tattttcatg gtcgtcgccg
     8641 taggcgattt caatatgggc gcgatggaaa acaagggttt gaacattttt aacaccaagt
     8701 tcgtcctcgc cgacagccgc accgccaccg ataccgattt cgaaggcatt gaatccgtgg
     8761 tcggacacga atatttccac aactggacgg gcaaccgcgt aacctgccgc gactggttcc
     8821 agctttcgct gaaggaaggg ctgaccgtgt tccgcgacca agagttttcc ggcgaccgcg
     8881 ccggccgcgc cgtgcgccgc atcgagaaca tccgcctgct gcgccagaac cagttccccg
     8941 aagacgcagg cccgaccgcc catccggtgc gccccgtcag ctatgaggag atgaacaatt
     9001 tctacaccat gaccgtttat gaaaaaggcg cggaagtggt gcggatgtat cataccctgc
     9061 tcggcgaaga gggcttccaa aaaggcatga agctatattt ccaacgccac gacggacagg
     9121 cagtgacctg cgacgatttc cgcgcggcga tggcggatgc gaacggcatc aatctcgacc
     9181 agttcgcctt gtggtacagc caggcgggca cgcccgtttt ggaagccgaa ggccgtctga
     9241 aaaacaatgt tttcgagtta accattaaac aaaccgtgcc gcccacgccc gatatggcgg
     9301 acaaacagcc gatgatgatt cccgtcaaag tcgggcttct gaaccgcaac ggcgaagcgg
     9361 tggcattcga ttatcagggc aaacgcgcaa ccgaagccgt gttgctgatg accgaagccg
     9421 aacaggcctt cccgctcgaa ggtgtaaccg aagccgtcgt tccctcgctg ctgcgcgggt
     9481 tcagcgcgcc ggtgtatctg aactatccgt acagcgacga cgacctgctg ctcctgctcg
     9541 cccacgacag cgacgctttc acgtgctggg aagccgccca aacgctctac cgtcgcgccg
     9601 tcgccgccaa ccttgccgcg ctttcagacg gcatcgggtt gccgaaacac gaaaaactgc
     9661 ttgccgccgt cgaaaaagtc atttcagacg acctcttgga caacgccttc aaagccctgc
     9721 ttttgggcgt gccgtccgaa gccgaactgt gggacggcac ggaaaacatc gacccgctgc
     9781 gctaccatca ggcgcgcgaa gccttgttgg atacgcttgc cgtccgcttt ctgccgaaat
     9841 ggcacgaatt gaaccgtcag gcggcgaagc aggaaaacca aagttacgaa tacagccccg
     9901 aaaccgccag ctggcgcacg ctgcgcaacg tctgccgcgc cttcgtcctg cgcgccgacc
     9961 ccgcgcacat cgaaactgtt gccgaaaaat acggcgaaat ggcgcaaaac atgacccacg
    10021 aatggggcat cctgtccgcc gtcaacggca acgaaagcga tacgcgcaac tgcctgctgg
    10081 cgcagtttgc cgacaagttt tcagacgacg cgctggtgat ggacaaatat ttcgccctta
    10141 tcggctcaag ccgccgcagc gacaccctgc aacaggttca aaccgccttg cagcatccga
    10201 aattcagtct cgaaaacccc aacaaagccc gttcgctcat cggcagcttc agccgcaacg
    10261 tcccgcattt tcacgcacaa gacggcagcg gctaccgctt catcgccgac aaagtcatcg
    10321 aaatcgaccg cttcaacccg caggtcgccg cccgcctggt gcaggcgttc aacctctgca
    10381 acaagctcga gccgcaccgc aaaaacttgg tgaaacaaga attgcagtgc attcgggcgc
    10441 aggaaggatt gtcgaaagac gtgggcgaaa tcgtcggcaa gattttgggt tgaggcagtc
    10501 aaacagaaaa aac
//
LOCUS       DAXFHF010000067         9863 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXFHF010000067 DAXFHF010000000
VERSION     DAXFHF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.067"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..1036)
                     /locus_tag="ACFIRM_001735"
     CDS             complement(<1..1036)
                     /locus_tag="ACFIRM_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC3029312.1"
                     /translation="MILGLFIRNFKTYQGMNFVPLSDNHKFCGLLGKNGIGKSSVLEA
                     LDCFFNGKEWNLHFTVKRNGLKVRPYIVPIFSINKDTKIPEEVKSIITKLDNLFRTTP
                     KEKLNFGSTHSSVKDTFISLRDSIINNPLFDNDYVFPIGIDHNKKATSAIFDSLLLDD
                     ISAEDNDTENRDHLIDTQLKAVYTYLVENLEYIYIPKDIDSELFTRLENQEIQTLMGE
                     KLDHILRDRITETAISEINKGLTDFINEISNELDGYVYRTSTDRQQNLRKSDIHKLII
                     DSFFNIRKLHKRVNESNNYVEISSLSSGEKQKAIIDVAKGLLENHRADGKNLIVGIDE
                     PESSLHVSACF"
     gene            complement(1047..3899)
                     /gene="uvrA"
                     /locus_tag="ACFIRM_001736"
     CDS             complement(1047..3899)
                     /gene="uvrA"
                     /locus_tag="ACFIRM_001736"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC3029313.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILRPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEQVAKIKGSYTGKYLKVALNK"
     gene            4053..4832
                     /locus_tag="ACFIRM_001737"
     CDS             4053..4832
                     /locus_tag="ACFIRM_001737"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC3029314.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            complement(5372..7648)
                     /gene="znuD"
                     /locus_tag="ACFIRM_001738"
                     /gene_synonym="tdfJ"
     CDS             complement(5372..7648)
                     /gene="znuD"
                     /locus_tag="ACFIRM_001738"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC3029315.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            8059..8649
                     /locus_tag="ACFIRM_001739"
     CDS             8059..8649
                     /locus_tag="ACFIRM_001739"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC3029316.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            8713..9741
                     /gene="trpD"
                     /locus_tag="ACFIRM_001740"
     CDS             8713..9741
                     /gene="trpD"
                     /locus_tag="ACFIRM_001740"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC3029317.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
BASE COUNT         2240 a         2246 c         2739 g         2638 t
ORIGIN      
        1 caaaacatgc agaaacatgt aatgaggatt caggctcatc aattccaacg attaaatttt
       61 taccatcagc tcgatgattt tccagcaatc cttttgctac atcaataatt gctttttgct
      121 tttctccaga acttaatgag ctaatttcta cataattgtt tgattcatta acccttttgt
      181 gtaactttct tatattaaaa aaagagtcaa taattaattt atgaatatca ctttttctta
      241 aattttgttg cctatcagtg gaggttctat atacataacc atctaactca ttgctaattt
      301 cattgataaa atcagttaga cctttattta tttcactaat tgcagtttct gtaatcctgt
      361 ctcttaaaat gtgatctaat ttttcaccca ttaaagtctg aatttcttga ttttctagtc
      421 gggtaaataa ctcagaatct atatctttag gaatatatat atattccaaa ttttctacta
      481 agtaagtata aacagctttt aattgagtat cgattaggtg gtctctgttt tctgtatcat
      541 tatcttctgc tgaaatatca tccaagagca atgaatcaaa gattgcagaa gttgcttttt
      601 tgttatggtc gatacctatt ggaaatacat agtcattatc gaataaagga ttgttgataa
      661 tactatctct tagacttata aaagtgtctt taactgatga gtgagtacta ccaaagttaa
      721 gtttctcttt tggagttgtt ctaaataaat tatcaagttt agtaataatg gatttcacct
      781 cttcaggaat tttagtgtcc ttatttatag agaaaatagg cacaatataa gggcgaactt
      841 ttagcccatt ccttttaaca gtaaaatgaa gattccattc tttgccatta aaaaaacaat
      901 ctagagcttc taatactgaa cttttaccta tcccattttt tcctagtaat ccacaaaatt
      961 tgtgattatc tgataaagga acaaaattca taccttgata agttttaaaa tttctaataa
     1021 ataatcctaa aatcatttga gcatccttac ttattcaacg ctactttcaa atacttccca
     1081 gtataactcc ccttaatttt agccacttgt tcagggctac ctttcgcgat aaccttaccc
     1141 ccaccatcgc cgccttccgg ccctaagtcc acaatccaat ccgcggtttt aattacatcc
     1201 agattatgct cgataatcac aatcgagttg cctttgcctt tcagacggcc tatgacttcc
     1261 agcagcaggg cgatgtcggc gaagtgcagg ccggtggtgg gttcgtcgag gatgtagagc
     1321 gttctgccgg tgtcgcgttt ggagagttcc aaggcgagtt tgacgcgctg ggcttcgccg
     1381 ccggagaggg tggtggcgga ttggccgagg cggatgtagc cgagacctac gtccatcagg
     1441 gtttgcagtt tgcgcgatac ggtggggacg gcgtcgaaaa attcgcgggc ttcttcgacc
     1501 gtcatgtcga ggacttggct gatgtttttg cctttgtatt ggatttcgag ggtttcgcgg
     1561 ttgtagcgtt tgccgtggca gacttcgcag gggacgtaca cgtcgggcag gaagtgcatt
     1621 tcgactttga ttacgccgtc gccttggcag gcttcgcagc ggccgccttt gacgttgaag
     1681 gagaatctgc cgacgttgta gccgcgttcg cgcgagaggg gtacgccggc gaagagttcg
     1741 cggatggggg taaacaggcc ggtgtaggtg gcggggttgg agcgcggggt gcggccgatg
     1801 ggggattggt cgacgttgat gactttgtcg aggtgttcga ggccgcggat gtcgtcgtat
     1861 ggggcgggct cttcttgggc gcggttgagt tcgcgggcag tgattttggc gagggtgtcg
     1921 ttaatcaggg tggatttgcc gctgccggat acgccggtga tgcaggtaat caaaccaagc
     1981 ggcaactcga gggttacgtt tttgaggttg ttgccgcgcg cgcctttgag gacgagcatt
     2041 cggccgggat tgacgggcgt gcgttcagac ggcacggcaa tggatttttt accgccgagg
     2101 tattgtccgg taacggattt ttcgcatttg gcgacatttt cgggtgtgtc ggcaatcagt
     2161 acgttgccgc cgtgttcgcc cgcgccgggg cccatatcga cgacgaaatc ggcttcgcgg
     2221 atggcgtctt cgtcgtgttc gaccacaatt acgctgttgc ccaaatcgcg caggcgtttg
     2281 agggtggcga gcaggcggtc gttgtcgcgc tggtgcaggc cgatggaggg ttcgtccaaa
     2341 acgtacatca cgccggtcag gccgctgccg atttggctgg cgaggcggat gcgctgggct
     2401 tcgccgccgg aaagggtttc ggcggaacgg cttaaattca ggtaatccag cccgacgtta
     2461 atcaagaagc cgagccgctc ggtgatttct ttgaggattt tttcggcgat ctgttttttg
     2521 ttgccgtcca aatccagcgt ttcgaagaat cggtgggttt tggtcagcgg ccaggcggag
     2581 acttcgtgca acggttcgcc gccgacgtaa acgtagcgtg cttctttgcg caaacgtgcg
     2641 ccgccgcagc tcgggcaggc acggtgattt tggtactcgc gcagtttttc gcgcacggtt
     2701 tcgctgtcgg tttcgcggta gcggcgttcg agattgggga tgatgccctc gaaggcgtgg
     2761 ctgcggttga aggtggtgcc gcgttcggac aggtaggtga aatcaatgac ttctttgccc
     2821 gagccgtgca acacgacttt tttgactttt tcaggcaaag tttcccaagc gacgttgaca
     2881 tcaaatttat aatgatgcgc cagcgattga atcatttgga aatagaattg gttgcgtttg
     2941 tcccaaccgt caatcgcgcc ggttgccagc gacaattcgg gatgggcgac tactttttcg
     3001 gggtcgaaga aattggtgtt gcccaagccg tcgcaagtcg ggcaggaacc catcgggttg
     3061 ttgaacgaaa agaggcgcgg ttccaattcg ggcaggctgt acgaacacac ggggcaggca
     3121 aaacgcgcgg aaaaccaatg ttcttcgccg ctgtccattt ccattgccag cgcgcgctcg
     3181 ttgccgtggc gtaatgcggt ttcaaaactt tccgccagtc gttgtttgat gtcggctttc
     3241 actttcacgc ggtcgatgac gacgtcgata ttgtgtttga tgtttttttc cagcttcggt
     3301 acttcgtcaa gctgatagac ttcgccgtcc acgcgcacac gggcaaagcc ttgcgcctgc
     3361 aagtcggcaa agaaatcgac aaactcgccc ttacgctcgc gcaccgccgg cctcagaatc
     3421 atcacgcgcg tgtcttccgg cagttttaat acggcatcga ccatttgaga tacggtttgg
     3481 ctggatagcg gcagcttgtg ttcggggcaa tacggcgtgc cgacacgggc gtataaaagg
     3541 cgcaggtagt cgtggatttc cgttaccgtg ccgacggtgg aacgcggatt gtggctggtg
     3601 gatttctgct cgatggaaat cgcaggcgac aggccttcaa tcaaatcgac atcgggtttg
     3661 tccatcatct gcaaaaactg ccgcgcatag gcagacaggc tctcgacata acgccgctgc
     3721 ccttcggcat ataaagtatc aaacgccagc gatgatttgc cgctgcctga caatcctgtt
     3781 accaccacga gcttgtggcg gggaatgtcc aaatcgatgt ttttcaaatt atgcgtgcgc
     3841 gcgccgcgaa tgcggatggt gtcgttgtcg tgcgaatgtc ggggatgatg gttgcacata
     3901 atggatgccg cctgaaaaaa taaaggaaaa ccgatattgt agcactttct cggatgccgt
     3961 ctgaagccgc gttcagacgg catttgtcgg cggaacgcgg cggattccgt tataatgtcg
     4021 ctatttaata tatttgaata aaaggatgac aaatgaaccg tctttatccc cacccgatta
     4081 tcgcccgtga gggctggccg attatcggcg gcggtttggc tttgagcctg ctggtgtcga
     4141 tgtgctgcgg ctggtggtct ttgccgtttt gggtgtttac cgtatttgca ttgcagtttt
     4201 tccgcgaccc tgcgcgtgaa attccgcaaa atcctgaagc ggtgttgagt ccggttgacg
     4261 gccgtatcgt ggtggtcgag cgcgcacgcg atccgtatcg tgatgtcgat gctttgaaaa
     4321 tcagtatttt tatgaacgtg ttcaacgtgc attcgcaaaa atcgcctgcc gattgtacgg
     4381 taacgaaagt ggtctataac aaaggcaaat tcgtgaatgc ggatttggac aaagccagca
     4441 cggaaaacga acgcaacgcg gttttggcga ctacggcttc cggtcgtgaa attacttttg
     4501 ttcaagtggc cggtctggtg gcacgccgta ttttgtgtta cacccaagca ggtgcgaaac
     4561 tgtcccgcgg cgaacgctat ggctttatcc gcttcggttc gcgcgtggat atgtatctgc
     4621 ctgtcgatgc gcaggcgcaa gtggcgattg gtgataaagt aaccggcgta aaaaccgtat
     4681 tggcgcgttt gccgctgact gattctcaag ccgatcctgt ttcacaagct gcttcggttg
     4741 aaacagcggc aaacccatct gccgaacagc agcaaatcga agcggcggcg gctaagattc
     4801 aggcggctgt gcaagatgtg ttgaaagatt aattttgcga actgaaatag aaaatatcag
     4861 taccatcatt cacacgaatg aggaagtttg gttttttgaa tttttgctaa tgttcacacc
     4921 gttatgttca cgaaaatggg aatccgggaa cttaacgtta cggtgattta tcagaaataa
     4981 cagaaaccga acgaattgga ttcccgcctg cgcgggaatg acgactcatt agttacctaa
     5041 aacttaaaaa acagaaacct ttacgccgtc attcccacga aagtgggaat ccgggaactt
     5101 aacgttacgg taatttatcg gaaacggctg aaaccgaatg aattggattc ccgcctgcgc
     5161 aggaatgatg ggatcttggg tttttgcttt tgatttttct gcttttgcga gaatgacggc
     5221 gtgaaagcaa gaatgatgaa acaaagaaaa tgggaatgat ggcacagtgg tttgttcctt
     5281 gtctttgcca tatttcctaa caagttgatt aaaaagaaaa aaaggttttc agaatgccgt
     5341 ctgaaaacct tttttgtttg cctgtccgat tttaaaactt cacgtttacg ccgccggtaa
     5401 agctgcggcc catttgcggc gtatcagaga ggaagctgct gtgggcgtaa acggattggt
     5461 tgagcaggtt gtcggctttg acgtaccaat tccactcgcc atagcgcgta ttgcggcggt
     5521 agtttgcacc gaggttgagc atatggtgtc cgggcgtacg cgtttcgtag cgggcgagtt
     5581 tgttttgggc gaacacgcgg tagtagtcca aattggcatc gatacggtcg gttagcgagg
     5641 ttttcaggtg gaagccgagg cgcgcagccg gaatgcgggg ggcattttgg tcgtcctgcg
     5701 cgatgaaggg acgtttgccg taagcatctt ccctgcctgg tagggacggt aggtttttca
     5761 gacggcctcg tacatagtcg ccggaaacgc cgatgcggta gcgcggtgtc ggtttgaagt
     5821 agatttcgcc ttccgcgccg tagaagtcgg caccggattg gttgtagcgc acgagcttca
     5881 tttcgctgtc gtcttcgatg gatttggggc cgcgtccgtc gtttaaggtt tgggcgtaaa
     5941 tgtagttgcc gaatcggttg cggtaggctg ccagattgta ttgccagcgg tcgccttcgt
     6001 agcccagcgc gagttcgata ttgttggaac gctctttgtt gaggtgtttg ttgccgactt
     6061 caaaggtgtt ggtggcgacg tgcttgccgt gtgcgtacag ttcttgcgtt gacggcaggc
     6121 gttcctgatg ggaggcggtc aggctgagtt tgtggtgtgg cgtgaaatac cagttgcccg
     6181 aaagtgcgaa cgagcgggcg gtttggcggt gcgcgccgag gtcgggcagg ggctggttgt
     6241 agtagttttc tcgatcaatc aatgctttgt cgtaccggat ggaggctttt tgtttttcca
     6301 cgcgtacgcc gccttcaagc gtgaagttgt cccaatttgc ctgttctaca ccgaaaaagc
     6361 tgtaatggcg gacattgttg tcaatcaaca tcggttgttg gacggtttcg ggaatggcgg
     6421 aaagcgcgct ggatttttgt cccaaatatt gcacgcccca gctgcctttc agacggccta
     6481 tgggttggtg gcgcaactcg atacgggcgt tgtgtgtttt gttgttgaag aagttttcta
     6541 ctgcatcgcc tgctttttcg tcgtggtggt agtcattgcg gttcagatgt acgcgcaggg
     6601 cttcaaaacc ggggaatggc tgcttccatt cggcgcggag ttcgtagcgt ttgttgcgaa
     6661 ggtctatcca cggtttgccg ttgtgggtgt gtgcgtgtgc accgtcgccg tcgtggaagc
     6721 cgcagctcaa gcccggattg tcgtaatcga tgtcttcttc ggtcaacaag tgcggataaa
     6781 gctgcaaata gcgtttgttg atcaaactct tttgccagat gatgtcggcg tggcaatcat
     6841 cgtattcgtg gctgtgggca ggcaggccgt agcggtcgcg acggtcgctg tatgctgcgc
     6901 cgataaagcc tttttcgcct acccaagaca gcccgatgct gcccgtttgc gaatcggcat
     6961 ggctgtcggg caggcgtttc agattgcggt aacgcggtac ggcgtaatcg cccgatttgc
     7021 ggtacaagcc ttcggtatgc agcacgaagt ttttgcccag tccgatattg atgcctgcgg
     7081 atgtcagttt ttctaaattg ccgctgctca aacgcaatcc ggcttcgccc gatacgccgt
     7141 tttcaggcat tttttcgggg atttttccat cggcaacatc gaccagcccc gccacattgc
     7201 ccgagctgta caagagcgta accggcccgc gcaggatttc aacctgttgc gacaaggcgg
     7261 tatctaccat aatggcgtga tcgggagaaa agtccgccat atcgcccgtt tcgccgtgat
     7321 ggttcaatac tttaatccgt ctgcccgttt gaccgcgaat aacgggagcg gatgcgccgc
     7381 cgccgtattg cgaggcatga atgcccggta cgccgtccaa agcgtcgccc aagttgacgg
     7441 ctttttggcg caaagtatcg ccggagatga ttttgtcgga ggcggtcgaa gtgtgcagca
     7501 gccccgaagt cgcgcgcgga cggcttttgc cgacgacgct gaccgtttcc aagcccaccg
     7561 attgctcagt ttcatgcgct tgggcgagga ggggtgtgtt gattaaaaga attgataaaa
     7621 caatgggttt gagtgtgatt tgtgccattt tggcttctcg tcgcatttca aaattttgtt
     7681 attatataac attgcatttt ttatatcata agattttgag aatactcaga gggcataggc
     7741 aaaagttttt caaatgacac ggttgtgata tttaatggcg ttaatttgta ttaaccctaa
     7801 ttttagggaa gttaatgatg tgaacacgaa cgccgctata tgttagctat cggataacca
     7861 aaatttatga gggcataaaa acggtacgtt cccgaacggt cgaaaaggtg gcaaaatggc
     7921 gtacttgtca gaacgcgagg ctctgcgcca gattcacgag ggcgcatcgg gcacgctaag
     7981 atataagggg gtatggatgg gggtatcgga aatgcagtta ataatagaca aattataaat
     8041 caataggtta atcacaaaat gcttttgttt atcgacaatt acgacagttt tacttacaac
     8101 atcgtccagt atttcgcaga attggggcag gaagtcgccg tgcgccgcaa cgatgatatt
     8161 acgttggagg aaatcgaggc attgaatccg caatatctcg ttatcggtcc cggaccgtgt
     8221 tcccctaagg aggcgggtat ttcagtagaa gccatgcgcc attttgccgg ccggctgccg
     8281 attatgggcg tgtgcctcgg gcatcagacg ataggcgaag cgttcggtgg agatgtggta
     8341 cgggcaaaaa ccttgatgca cggtaaggtg tcgcccgtgt cccattcggg caagggtatg
     8401 tttaagggtt tgcccaatcc ggttacctgt acgcgttatc acagcctcgt tatcgaacgc
     8461 ggcacgctgc cggattgctt ggaaatcacg gcgtggacgg aagacggcga aattatgggc
     8521 gtgcgccata aggaatatgc cgtcgagggc gtgcagttcc accccgaagc cctcttgacc
     8581 gaacgcggac atgatatgtt gaacaatttt ttagttgaat ttcaaaactt caaaccgcaa
     8641 aaaatctgac gtgatgccgt ctgaagccct tcgggcggca ttttcgtccg aatattgaac
     8701 ggaggacaaa aaatgattac accgcaacag gctatcgaac gattaatcag caataacgag
     8761 ttgttttacg atgaaatgac cgacttgatg cgccaaatga tgagcggaaa agtgccgccc
     8821 gaacaaattg cggcgatttt gaccggcttg cgtatcaagg ttgaaaccgt ttccgaaatc
     8881 accgccgccg ccgccgtgat gtgcgagttt gcgtcaaaag tgccgctgga ggatgcggac
     8941 gggctggtcg atatcgtcgg tacgggcggg gatggcgcga aaaccttcaa tatttcgacg
     9001 acttcgatgt ttgttgctgc agcggcaggc gcaaaggttg ccaaacacgg aggccggtcg
     9061 gtctcttcct ccagcggtgc ggctgacgtg atggagcaaa tgggcgcaaa cctcaacctg
     9121 actcccgaac agattgccca aagtatcagg cagaccggta tagggtttat gttcgcaccc
     9181 aatcaccaca gtgccatgcg ccatgtcgcc ccggtgcgcc gttcgctcgg tttccgaagc
     9241 attttcaaca tattgggtcc gttaaccaat cctgcgggcg cgccgaacca gcttttgggc
     9301 gtgttccaca ccgatttgtg cggcattttg tcgcgggtct tgcaacaact cggctcaaaa
     9361 cacgttttgg tcgtttgcgg ggagggcggt ttggatgaaa ttacactgac gggcaaaacg
     9421 cgcgttgccg aactcaaaga cggaaaaatc agcgaatacg acatccgccc agaagatttc
     9481 ggtatcgaaa cccgccgcaa tttggatgaa atcaaagttg ccaatactca ggaatctttg
     9541 ttgaaaatga acgaggtact ggacgggaaa gaaggggcgg cgcgcgatat cgtattgctt
     9601 aataccgccg ccgccttata tgccggaaat atcgctgctt cgctttcaga cggcatatct
     9661 gccgcacggg aaggcatcga ttcaggtagg gcgaaggcga aaaaagagga gtttgtcggt
     9721 tttacacggc aattcgccta agccggcaaa cttgatataa agcaacaaat gccgtctgaa
     9781 cggcggaatt ggcgtttcag acggcatgag gcctttgcaa aaaagccctt ccttcgacat
     9841 ccgaaaccca aacacaggtt ttc
//
LOCUS       DAXFHF010000068         9362 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXFHF010000068 DAXFHF010000000
VERSION     DAXFHF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.068"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..208
                     /locus_tag="ACFIRM_001741"
     CDS             <1..208
                     /locus_tag="ACFIRM_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029318.1"
                     /translation="PSPPPRGREWVSADFSVAGGLKNNLNLQPLISGRLKKNARNINS
                     GNFSGSLYRKAGGTNAASIFSDDL"
     gene            469..1002
                     /locus_tag="ACFIRM_001742"
     CDS             469..1002
                     /locus_tag="ACFIRM_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029319.1"
                     /translation="MFSDIYLNPKNYFGIEKRLPFKAFWDNGGQCYLDLKVVDGKIIA
                     LCGQLINYTNTSIVNAAEEVYRSIIDYLISNEILIVRTKFSFKNIFSTNEKKYKEIEK
                     EINKFLYKNMDWYLFFNNDITLDPYTGNDLIWHVQIDPNDGGAQFLETYQSWEKLQQK
                     YPNITFSYDKNDLILTK"
     gene            1013..4228
                     /gene="carB"
                     /locus_tag="ACFIRM_001743"
     CDS             1013..4228
                     /gene="carB"
                     /locus_tag="ACFIRM_001743"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC3029320.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIIHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4429..5469)
                     /gene="queA"
                     /locus_tag="ACFIRM_001744"
     CDS             complement(4429..5469)
                     /gene="queA"
                     /locus_tag="ACFIRM_001744"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC3029321.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            5586..5843
                     /locus_tag="ACFIRM_001745"
     CDS             5586..5843
                     /locus_tag="ACFIRM_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC3029322.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            5825..6286
                     /gene="accB"
                     /locus_tag="ACFIRM_001746"
     CDS             5825..6286
                     /gene="accB"
                     /locus_tag="ACFIRM_001746"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC3029323.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6400..7761
                     /gene="accC"
                     /locus_tag="ACFIRM_001747"
     CDS             6400..7761
                     /gene="accC"
                     /locus_tag="ACFIRM_001747"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC3029324.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            7865..8752
                     /gene="prmA"
                     /locus_tag="ACFIRM_001748"
     CDS             7865..8752
                     /gene="prmA"
                     /locus_tag="ACFIRM_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC3029325.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            8770..9333
                     /gene="orn"
                     /locus_tag="ACFIRM_001749"
     CDS             8770..9333
                     /gene="orn"
                     /locus_tag="ACFIRM_001749"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC3029326.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2339 a         2724 c         2423 g         1876 t
ORIGIN      
        1 ccccagccct cccccacggg ggagggagtg ggtttcggca gatttttcgg ttgccggtgg
       61 tttgaaaaat aacttaaatt tgcaaccgtt gatttcaggt cgtctgaaaa agaatgcccg
      121 aaatatcaac agcgggaatt tttcaggcag cctttatcgc aaggcaggtg gaacaaacgc
      181 cgcgagcatt ttttcagacg acctttgaac tcatcggcag agagtgtgcc gcaaggcgcg
      241 catgcggtgg gtcggggtta cagggaaaat gaagaacgcg tgcgtgcgta ccgcacatac
      301 cctacatgcg ggctacggct tgctacgata cgggggtttc gatatacaag ttgggttagc
      361 aaacccaaca ttttagacaa ttaagcggtt tgtgttaggt tttcaatcca aactacgctt
      421 gccacatgag cgagctatga cttaccacac ttaaccaagg agttagtaat gtttagtgat
      481 atctacttga acccaaaaaa ctattttggt atagaaaaaa gattgccttt taaggcattt
      541 tgggataatg gcggacaatg ctatctagac ttaaaagtag tagatggaaa aataattgcg
      601 ctttgtggac aacttattaa ttatacaaac acgtctattg taaatgctgc agaggaagtt
      661 tatcggtcaa taattgatta tttaatttcc aatgagattt taattgttag aactaaattt
      721 tctttcaaaa atattttttc aactaacgaa aaaaaatata aggaaataga aaaagaaata
      781 aataaattct tatataagaa tatggactgg tatttatttt ttaacaatga cataacatta
      841 gatccatata cagggaatga tttaatatgg catgtgcaaa tcgatccaaa tgatgggggt
      901 gcgcaattct tggaaactta tcaatcatgg gaaaagttac agcaaaagta tcctaatatt
      961 acattttcgt atgataaaaa tgatttaatt ttaactaaat agagacccac ccatgcccaa
     1021 acgtaccgac ctaaaatcca tccttatcat cggcgccggc cctatcgtta tcggtcaggc
     1081 ctgcgaattt gactattcgg gcgcacaggc ctgcaaggct ttgcgtgaag aaggctataa
     1141 agtcattctg gtgaattcca accccgccac gatcatgacc gaccctgaaa tggcggatgt
     1201 tacctacatc gaaccgatta tgtggcagac ggtggagaag attatcgcca aggagcggcc
     1261 cgatgcgatt ctgcccacga tgggcggtca gaccgcgctg aactgtgcgc tggatttggc
     1321 gcgtaacggc gtgctggcga aatacaatgt cgagttaatc ggcgcaacgg aagacgcgat
     1381 cgacaaggcg gaagaccgcg gccgctttaa agaagcgatg gaaaaaatcg gcctctcttg
     1441 cccgaaatct tttgtctgcc acaccatgaa cgaagccttg gcggcgcaag aacaggtcgg
     1501 ctttccgacg ctgattcgtc cgtctttcac gatgggcggt tcgggcggcg gcatcgccta
     1561 caataaggat gagtttttgg cgatttgcga acgcggtttc gatgcgtcgc ctacgcatga
     1621 gctgctgatt gagcagtctg tgctcggctg gaaagagtac gagatggaag tggtgcgcga
     1681 taaggcggac aactgcatca tcatctgttc gattgaaaac ttcgacccga tgggcgttca
     1741 tacgggcgac tcgattacgg ttgcgccggc gcaaacgctg acggacaagg aataccaaat
     1801 catgcgcaac gcttcgttgg cggtattgcg cgaaatcggc gtggacacgg gcggctcgaa
     1861 cgtgcagttt gcggtgaacc ctgaaaacgg cgagatgatt gtgatcgaga tgaacccgcg
     1921 cgtgagccgt tcgtccgcgc tggcttccaa agcaacgggc ttcccgattg cgaaggtggc
     1981 ggcgaagctg gcggtcggct ttacgctgga cgagttgcgc aacgacatca ccggcggccg
     2041 cacgcccgcg tcgttcgagc cttccatcga ctatgtggta accaaaatcc cgcgtttcgc
     2101 gtttgaaaaa ttccctgccg cagacgaccg cctgaccacg cagatgaaat ctgtgggcga
     2161 agtaatggcg atgggccgta ccattcaaga aagcttccaa aaagccctgc gcggtttgga
     2221 aaccggcttg tgcggtttca atccgcgcag cgaagacaaa gcggaaatcc gccgcgaact
     2281 ggccaaccct ggccccgaac gtatgctgtt tgtggcagac gcgttccgcg cgggcttcac
     2341 gccggaagaa atccacgaga tctgcgccat cgacccttgg ttcttggcgc aaatcgaaga
     2401 cttgatgaag gaagaaaaat cggtttcaga cggccagttg caagatttgg attacgccgc
     2461 cctacgccgt ctgaaacgca aaggcttctc cgacaaacgt ttggcacaat tgttgaacgt
     2521 aagcgaaaaa gaagttcgcg aacaccgcta cgcgctgaag ctgcatccgg tttacaaacg
     2581 cgtcgatacc tgcgccgccg agttcgccac cgaaaccgcc tatctttact ccacttacga
     2641 agaagaatgc gaatcccgtc cttccgaccg taaaaaagtg atgattctcg gcggcggccc
     2701 gaaccgcatc ggtcagggca tcgagtttga ctactgctgc gttcacgccg cactcgccct
     2761 gcgcgaatcg ggctttgaaa cgattatggt caactgcaac cccgaaaccg tgtccaccga
     2821 cttcgacacc agcgaccgcc tctatttcga gccgctgaca ttggaagacg tgttggaaat
     2881 cgtccgcacc gaaaacccgt ggggcgtgat tgttcattac ggcggtcaaa ccccgctgaa
     2941 actcgccaac gcattggttg aaaacggcgt gaacatcatc ggcacatccg ccgacagcat
     3001 cgatgccgcc gaagaccgcg aacgcttcca aaaagtgttg aacgacttag gcctgcgcca
     3061 accgcccaac cgcatcgccc acaacgaaga agaagccctc gtcaaagccg aagaaatcgg
     3121 ttatccgctg gtcgtgcgcc cgtcttatgt tttgggcggc cgcgccatgc agattatcca
     3181 ctccgccgag cagttgcaaa aatatatgcg cgaagccgtg caggtttccg aagacagccc
     3241 cgtgctgctc gatttcttcc tcaacaacgc gattgaagtc gatgtggact gcgtttcaga
     3301 cggcaaagat gtggttatcg gcggcatcat gcagcacgtc gaacaggcgg gcatccactc
     3361 cggcgactcc ggctgctcgc tgccgccata ctccttaagc gaagaaatcc aagacgaaat
     3421 ccgccgccaa accaaagcga tggcgtacgc gctgggcgtg gtcggactga tgaacgtgca
     3481 gtttgccgtg caggacggcg tggtgttcgt gttggaagtg aacccgcgcg ccagccgtac
     3541 cgtccccttt gtctccaaag ccaccggcgt gccgctcgcc aaagtcggcg cgcgctgcat
     3601 ggcaggcatt tccctgaaag aacaaggcgt ggaaaaagaa gtcgtccccg atttctatgc
     3661 cgttaaagaa gccgtgttcc cattcatcaa attcccgggc gtggatacga ttttgagtcc
     3721 ggaaatgcgc tccaccggcg aagtgatggg cgtgggcgca agtttcggcg aagcctacta
     3781 caaagcccaa ctcggcgcgg gcgaacgcct caacccgacc ggcaaaatct tcctctccgt
     3841 gcgcgaagaa gacaaagaac gcgtcattaa aaccgccaaa aacttccaag ccttaggcta
     3901 cggcatctgc gccacgcgcg gcacggcgca atacctgacc gaacacgggc tgattgtgca
     3961 ggccatcaac aaagtccccg aaggccgccc gcacatcggc gacgcgctga aaaacggcga
     4021 aatcgcactg gtcgtaaata ccgtttccag cgatccgcaa tccgtgtccg acagccacat
     4081 catccgccaa agcgcattgc agcaacgcgt gccgcaatac accaccaccg ccggcggcga
     4141 agcgatgagc gaaggcgcga aaagccggga ttatctgggt gtgtacagcg ttcaagagct
     4201 gcacggtcgt ttgaaaaacc gcaactgatg cctgaatcag gttgaacaat gccgtctgaa
     4261 gccgttttgc ggtttcagac ggcattttgt tgcacatagc cttcactttc ttttgacctt
     4321 taagcaccct aatttggggc ggtggattat ccaactgccg gacatccata aaaataccgc
     4381 ccgaaccacg gttcagacgg catctgcctt gcaacggaca gcagcagttt aaagcccgcc
     4441 cccttcgttc cgccccaaaa ccatcgcatc tccgtagctg aagaaacggt attcacgttc
     4501 aatcgcatga cggtacacgg cgcggatatg acccataccc gaaaacgcgc cgaccaacat
     4561 cagcagcgtc gatttcggca gatgaaaatt ggtaaccagc ctgtcgacaa cattaaaacg
     4621 gtagcccggc gtgatgaaaa tatcggtgtc gccctgtccg tctttcaaat atcccgttgc
     4681 gcgcgcggca gactcgaggg cgcgcatgga agtcgtgccg accgcccagg ctttgttccc
     4741 ccgggctttt gccgcctcaa cggcggcgac ggtttcagac ggcacttcaa accattcgct
     4801 gtgcattttg tgttcttcga ttttatcgac gcgcacgggt tggaatgtcc ccgcaccgac
     4861 gtgcagggtt acttctgcgg ttaccgcgcc tttgtctttc agacggcgca aaagttcttc
     4921 cgtaaaatgc aggcccgccg tcggcgcggc gaccgcgccc tgatatttgg cataaacagt
     4981 ttgataacgg ctgtcgtcgt ccgcatcggc ggcacgttcg atataaggcg gcaggggcag
     5041 gtgtccgttc tgttccaaaa gttcgtaaac ggtttgaccg ccttcaaaac gcaggcagaa
     5101 cagttcgccc tcacgcccga ccatcacggc acggataccg ccttcaaaca ccagccccat
     5161 accgggcttg ggggacttgg acgaacggat gtgcgccaat gcggtatggt tgtccaacac
     5221 acgctcaatc agggcttcga tcctgccgcc gctgtctttc tgcccaaaca gccgcgcctt
     5281 catgactttg gtgttgttga ataccaaaac gtcgcctgcc tcgacataat cgggcaaatc
     5341 gccaaacacc cggtcttgca gcggcatatc gggcagcgcg accaaaagcc ggctgctgcc
     5401 gcgcacctcg ggcggatgct gggcaatcag gtgttcgggc agggtaaagt caaaatctga
     5461 aatatccatt tttacactct cgttcgggca agccgccatt atacgcactt tagccctttt
     5521 tcagacggca tctttgcccg aaaaaccaac agattagaat aaacactctt gactcggggc
     5581 atcttgtgcg caaaatcaaa cttcctgcac atttccccca aaaaccgccg ttttttgata
     5641 ttttactgga catttaccga caacttcggg aaaatgaaca caatttcacg gtcgtttccc
     5701 accacaggaa aaccgtatcc gaacaccatt ccgcccggtt tgcgccgttg ccgcaagccg
     5761 gctgttttct gaaaaaccaa cgcaacaacc cgccggaaca ccggcagcct ttaaaggaac
     5821 agaaatggat ttgcgcaaat taaaaaaact gattgatttg gttgaagaat cgggtatcgc
     5881 cgaaatcgaa gtaaccgaag gcgaggaaaa agtccgcatt acccgtacca ttgcagctgc
     5941 ggcagctccc gtttacgccg cgcccgtacc tgccgccgcg ccggccgtaa cgccggccgc
     6001 cgcacccgtt gcggcatccg cgcccgccgc cgcgccggcc gcccgcgatt tgtccgacgc
     6061 tcaaaaatcg cctatggtcg gcacgttcta ccgcgcgccc ggcccgaatg ccgccgcatt
     6121 tgtcgaagtc ggtcaacagg tcaaagccgg cgacacgctg tgcatcatcg aagcgatgaa
     6181 gctgatgaac gaaatcgaag ccgaaaaatc cggcacggtc aaagaaattc tggtcgaaaa
     6241 cggtacgccc gtcgaattcg gcgaaccgct cttcatcatc ggataatcct gttttcagac
     6301 ggcataaact tccgatgccg tctgaaatgc tttcccccct tcagcgttcc cgcacccttt
     6361 ttttacggac gggttgccgg aaccgcagga aaggtcatca tgctgaaaaa agttttaatc
     6421 gccaaccggg gcgaaatcgc cttgcgcgta ctccgtgcct gccgcgaaat gggcattgcc
     6481 accgttgccg tgcattccga agccgacaaa ggcagcctgc acgtcaaact cgccgacgaa
     6541 tccgtgtgca tcggtcctgc cgcttctgcg caaagttatc tgaacattcc cgccatcatt
     6601 gccgccgccg aagtaacctt cgcggacgct gtccacccgg gctacggctt ccttgccgaa
     6661 aacgccgatt tcgccgaaca ggtcgaacag tccggcttta ccttcatcgg cccgaaaccc
     6721 gacaccatcc gcctgatggg cgacaaagtc tccgccaaac acgcgatgat agcggcaggc
     6781 gtgccctgcg tccccggctc tgacggcgca ttgcccgacg atgatgccga aatcctcaaa
     6841 atcgccgaca aagtcggtta tcccgtgatt atcaaagcat cgggcggcgg cggcgggcgc
     6901 ggtatgcgcg tggtcgagaa aaaagaagac ctcctccagt ctgtcgaaat gaccaaggct
     6961 gaagcgggcg cggcgttcgg caacccgatg gtttatatgg aacgctacct gcaacgcccg
     7021 cgccacgtcg aaatccaagt gcttgccgac gaacacggca acgccgtcta tcttgccgag
     7081 cgcgactgtt cgctgcaacg ccgccaccag aaagtcatcg aggaagcacc cgccccgttt
     7141 atcgatgaag aggcacgcaa aaaaatcggc aaagcctgta ccgacgcgtg caaacgcatc
     7201 ggctaccggg gcgcgggcac gtttgaattt ttatacgaag acggcgaatt tttctttatc
     7261 gagatgaaca cgcgcgttca ggtcgagcat ccggttaccg aactcattac cggcgtggac
     7321 atcgtccaag agcaactccg catcgcatcc ggcctgccgc tgcaatacaa acagaaagac
     7381 atcaaaatcg aaggccacgc cttcgagtgc cgcatcaatg ccgaagaccc gtacaacttc
     7441 atccccagcc cgggcccgat tgaaagctgc cacctgcccg gcggcttcgg catccgcgtg
     7501 gacagccaca tctaccaagg ctaccgcatc ccgccgtact acgacagcct gatcggcaaa
     7561 atctgcgtac acggcaaaac gcgtgaacag gcaatggcga aaatgcgcgt cgcactcgcc
     7621 gagctggcgg taaccggcat caaaaccaat acgccgttgc accgcgacct gttcgccgat
     7681 gcgggtttcc aagaaggcgg agtgagcatc cattacctgg agcactggct ggaagcgcgc
     7741 aaaaccaaac aggacaaata agccgccgcc gatatgccgt ctgaagccgc ccttccgcgt
     7801 tcagacggca tttcccttgc cccgccgtct gaaaccgatt tcgatatttc caagaaagcc
     7861 cgttatgccc taccaacaaa tcaccgtcaa cgtcaacgat gccgtcgccg aacgcctcgc
     7921 cgacgcgctg atggaacacg gtgcactctc cgccgccatc gaagatgcct gcgccggcac
     7981 gcaaaacgaa caggcgattt tcggcgaacc cggtatgccc accgaacaaa tctggcagca
     8041 gagcaaagtc atcgccctgt tcggcgaaca cgacgaagcc gccgccgtca tcgatgccgc
     8101 cgcacaagaa tgcgggttaa aagacttggc atacaccggc gaaaccatcg aagaccaaga
     8161 ctgggtgcgc ctcacgcaat cgcaattcga ccccatccga atttccgacc gcctgtggat
     8221 taccccctct tggcacgaag cccccgaagg ctgcgccgtc aacctccgcc tcgatcccgg
     8281 actcgccttc ggcaccggca gccacccgac cacgcgcctc tgcctcaaat ggctggatac
     8341 gcaactcaaa aacggcgaaa gcgtcctcga ctacggctgc ggttcgggca tcctgaccat
     8401 cgccgccctc aaactcggtg caggttccgc cgtcggcgtg gatattgacg aacaggccgt
     8461 ccgcgccggc agggacaacg ccgaacagaa caacgtcgat gcacaattct tcctgcccga
     8521 cagtctgcct caagggcaat tcgacgtagt tgtcgccaac atcctcgcca accctttgcg
     8581 tatgctcggc gaaatgcttg cagcccgcac caaacagggc ggacgcatcg tgttgtccgg
     8641 tttgttggat gaacaggccg aagaactcgg cggcatttac agccaatggt tcgatctcga
     8701 cccggcggaa actgacgaag gctgggcgcg cttgagcggc gtaaaacgct gaaacggaaa
     8761 ggaaacaccg tgcaggacaa aaacaacctc tgctggctcg atatggaaat gacggggctg
     8821 aatcccgaaa ccgaccgcat tatcgaagtc gcgatgatta ttaccgactc ggatttgaat
     8881 gtgttggcgc aatccgaagt ttacgccatc caccaaagcg acgatctgct cgacaatatg
     8941 gacgagtgga acaccgccac acacggcagg acggggctga cgcagcgcgt gcgcgaatcg
     9001 tcgcataccg aagccgaagt cgaacagaaa ctgctggact ttatgtcgga atggataccc
     9061 ggacgcgcca caccgatgtg cggcaactcc atccaccaag accggcgttt tatggtcaaa
     9121 tatatgccga aactggaaaa ctacttccac taccgcaacc tcgacgtttc cacgctgaaa
     9181 gaactcgcca aacgctggaa tccgcccatt gccaaaagcg tcgtcaaacg cggttcgcac
     9241 aaggcattgg acgacatttt ggagagcatc gaagaaatgc gccactaccg cgaacacttc
     9301 ctgatttccg ccccgaaagc cgaagcgcaa taagaaacaa acaatgccgt ctgaaacgct
     9361 at
//
LOCUS       DAXFHF010000069         9292 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXFHF010000069 DAXFHF010000000
VERSION     DAXFHF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.069"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            293..1147
                     /locus_tag="ACFIRM_001750"
     CDS             293..1147
                     /locus_tag="ACFIRM_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFC3029327.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGMPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1237..1695
                     /locus_tag="ACFIRM_001751"
     CDS             1237..1695
                     /locus_tag="ACFIRM_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3029328.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFIRM_001752"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFIRM_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC3029329.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5211)
                     /locus_tag="ACFIRM_001753"
     CDS             complement(3688..5211)
                     /locus_tag="ACFIRM_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC3029330.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVNMLDKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLKQTGLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAVYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(5518..6324)
                     /locus_tag="ACFIRM_001754"
     CDS             complement(5518..6324)
                     /locus_tag="ACFIRM_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3029331.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6376..6627)
                     /locus_tag="ACFIRM_001755"
     CDS             complement(6376..6627)
                     /locus_tag="ACFIRM_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029332.1"
                     /translation="MSSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6729..7499
                     /locus_tag="ACFIRM_001756"
     CDS             6729..7499
                     /locus_tag="ACFIRM_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029333.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7645..8814
                     /gene="metZ"
                     /locus_tag="ACFIRM_001757"
     CDS             7645..8814
                     /gene="metZ"
                     /locus_tag="ACFIRM_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC3029334.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8909..9115
                     /locus_tag="ACFIRM_001758"
     CDS             8909..9115
                     /locus_tag="ACFIRM_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC3029335.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2129 a         2276 c         2713 g         2174 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgt tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa tgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataagggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga cgatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agactcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gccttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccaac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggccagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca tagcgagttt
     4741 ggatttgttt tcagagccgc cgccttttgc cgcgcaggtt acttcgactt taccgcccgg
     4801 cacgatgctc atgtggatga cggcgggggt gttgtctttg gtgttttggc gtttgccggc
     4861 cggatcggcg aggacggaag cgcgcagggt gttgccttcc caagtgtagg cgcggcgtac
     4921 gccttcgtta accatctctt ccacgctcat gtccgcatcc cattgcacat ccataccgac
     4981 tttgaggaag acggttgcga tacctgtgtc ttggcagatg gggcggttgt tttcggcaca
     5041 catacggctg ttgaccaaaa tctgcgtcat cgcgtctttg gcggcgggat tttcttcctt
     5101 ctgccacgcc ttataaagcg cgtcgatgta gtcttttgga tggtagtagc tgatgaattg
     5161 gaaggcatcg cagatacttt gaataaagtc ttcttgcttg ataacggtca tgattgtgtt
     5221 ccttttcaag gttgtggcgg taagtgtctg ctgctgtttt aaacaacctt tttgaaggct
     5281 gttttttatc aggttgaaat agtgccagcg tttgtacggt gaagacaata cataattcta
     5341 atctcttgat tatcaatttg ttttaataaa atactgtttt attcggattt tgatatttgt
     5401 aatctttgcc tgttgctttg cggtagggag aaatcagcag gcagttcatt gtggaccggt
     5461 tgatgaacaa tgccgtctga aagggcttca gacggcattt tgttcggacg ggcgggctta
     5521 tttcgcagcc tgtccgccct ctttggcaaa atatttggcg tagatcttgt cgtattcgcc
     5581 gctttcgcgt actttttcca acgcatcgtt cagcattttg acggttgctt cgtcgccttt
     5641 gcgtaccgcg atgccgtagt gttcggtggt gaagtcgggc agggtaacga agtccattcc
     5701 tttggccggg ttgtttttca cataattggc gatgaccgcg ctgtcgctga ccacggaatc
     5761 caagccgccg ttttccagtt ctttgataat cagggggacg ttttcgaagc gcgcgatttt
     5821 cggattgtcg ttgcccaaga gtttggaaac ggagaaatcg cccgtgtggc cggtaaccac
     5881 gccgactttg ttcatctttt tcaaatcttc ggaagaagat acttttttgc ctttcggaac
     5941 gaggacgact tgggtgattt caaaatacgg gtcgctgaaa tccatagact gtttgcggtc
     6001 gtcggtaatg gttacgcccg acatcacaac gtccgcatcg ccgttgttca aggcggggaa
     6061 aaggctgtcc cacggctggt gtttgaattc gattttaaaa ttgcccgcct tcgccatcgc
     6121 gttcatcaaa tccacgtcga aaccttcgac attgcctttc gagtctaaag attcaaaggg
     6181 ggcaaactcg gcgttggaag ccacgcggta cactttgccg gggttggcgg caggcgcggc
     6241 ggcatctttg ccctggccgc cgcaggcaga cagcgcgagt gcggaacagg caagggcggc
     6301 ggcaatccat tttttcatat tcatcaaaat tcctttcggt aatcaaaaag cctaaaaaat
     6361 cagtttaaca taaaattaac gttccttttc acggaaaaac gcataagtaa cgtcgtggaa
     6421 caaatccgta tgcgggctgt cctcatagct caattctgcc aaggcgtcct caaaagcaag
     6481 ctgaatcgac tcgaccgcga tttcggcggt gtctgcggga agggcgcgga tcagggcttt
     6541 gaaggcggtg gacaggacgt ggttctgcat acgcagcaca tcgtttgctt cttccagata
     6601 ttccaaacgc tgttcgatac tgctcatgat ggatccttat gaatctgtta aaatgcgcca
     6661 ttatatcaaa ctgcgctttg ggcgcgggtt tgcccgcctg caaagattaa gaaccggaag
     6721 ccgaaaaaat ggatacaaac aatcaggata aagagccgaa ttgggaaaaa cgcctggaaa
     6781 acctgcgtca gacgcgccgt atgaataccg cgcctgccta tgtgccgctg accaaacctt
     6841 ctgaagtcgg aaaggaagag cccataccga aattggacga tgcaaaccga gaccgtgtgt
     6901 taaaggcgta tctgaagaaa tggcaggaag aacacaacag cgccgttgcc gattgcggcg
     6961 aggaagtcga aaccgacccg atgatcgtcc ttcaggaaaa ctggctcaac gcgcaggctt
     7021 ccctgcaaat gccggtttcg gaaaaacaca tcgaatcgcg gcggagaatc cggttcgatg
     7081 ccaaaaaaga cagtgccgaa tttgaaaccg cacagattga gggcggggaa aacgccgatg
     7141 cggcagaatc cggtacggcg gcagacgggg ttgcggaaaa cggcgaaggc ggtgcggaag
     7201 aggcgttgat gcccgtccaa atcaatatcc tgaacccgaa agccgtcaac cggcgcgaag
     7261 tgttctgcct gtccgaacag gagctgaccg aacggttgat caaacgcctg cgcccgcatc
     7321 tgaccgatac cgtcaacggc atgatccgta cggcggtgca gaagcagatg gcgctcttta
     7381 cttatcagct ccagcaaatg ctgcacgagc aggcgggtgc ggtagtcgaa gacgtgttgg
     7441 aacacgatgt ccgtaaaatc ctcaacgaca tcaagtacga attaaaatac aaacgctgat
     7501 tgccatgccg tctgaaagcc gtgttcagac ggcattttcc tgcccgtccg acttgaaagc
     7561 ctcatggaac aggcgcaaaa tattaataaa ttaaacaacg ggtgcacgcc ccgttgccga
     7621 acggacaaaa ccgaggaatc accgatgagc aaaaaacttc atccgcaaac gctcgccata
     7681 cgcggcggca aagaacaaac cggataccgc gaacacaatc aggcattgtt cctgaccagc
     7741 agcttcatgt gggacaacgc ccaacacgct gccgatttgt tttcaaaaaa aatcaaaggc
     7801 ttcacttata cccgtaccgc caacccgacc atcgcggcct ttgaaaaacg catagccgct
     7861 ttggaaggcg cggaacgcgc ggtcgccact tcgaccggca tgtccgcgat tcaggcggcg
     7921 tttttcacct tcctgcaggc gggcggccat gtggtttcca gccgcagcct gttcggcacg
     7981 accgtcggct ttatcaataa catcgttacc aaattcggca tcggagtgag ccgcgtgtcg
     8041 ccgactgata taaatgaatg gaaagccgcc gtcaaagcca acaccaaact gctgtttttg
     8101 gaaacgccgt ccaacccctt gggcgaagtg gccgacttgg aagccttggc ggaattggcg
     8161 cacggcatcg gcgcgctctt ggtggtggac aacagcctgc tgtcgcccgt cggctcgcag
     8221 cctttgaaac acggcgcgga tatttccgtt tcctccgcca ccaaagccat agacggacac
     8281 gggcgcgtga tgggcggcgt gttggcgggt tcggaagaac tgatggcgca ggtcgccgtt
     8341 tactgcaact cttgcggttt ggcgatgtcg ccgtttaacg cgtggcagtt gttgagcggc
     8401 gtggaaaccc tgtcgctgcg tatggaaaaa cagtttgaca acgccttgag aatcgcgcaa
     8461 tggctgcaag agcagccgca ggttcaagca gtgtactaca ccggattgcc cgaccatccg
     8521 caggcggagc ttatccgcaa acagcaaaac ggcggcggca tcgtcatcgg ttttgaagtc
     8581 gccgaccaag cagccgcgtg gaaagtcgtg gacggcgtgg aactcttttc ccgtaccgcc
     8641 aacctcggcg acgtgcgctc gaccatcacc aatccgtgga caaccacgca cggcaggatg
     8701 cagcccgaag aaaaactcgc cgccggtatc cgccccggac tggtgcgcct gtcggtcggt
     8761 ttggaatatg ttggcgattt gattgacgat ttgaaacagg cactcgcgcg ctgattgcgt
     8821 gtttcagacg gcatcggcgg aaaaatgcgg cagatgccgt ctgaaaccct tattgcgtat
     8881 ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg gtttttgccg attgggcgtt
     8941 gaaaatctgc ctgaccggtt tgattatttt tttgggtttt atcgtttgga atttgggcaa
     9001 ggagtccaaa gcgggcaaat tcggcatcgc cgttttgttt ttggttttgg gcttgggtgt
     9061 gttcggtttt gtgttcaaag agttattgat taagtttttg gtattgccca aataggtttg
     9121 ccgtctgtcg aaatgccgtc tgaaatcata aaaagtaatc tgcaccctaa aagtttgatt
     9181 taaccgccaa ctgattaagg tgcgggtttt tatgacaaaa taaaaatcat ctttccgcaa
     9241 tcattcccac ggatgcgaaa gcctgtcttt ttgaatcccg gataatttta ca
//
LOCUS       DAXFHF010000070         9201 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXFHF010000070 DAXFHF010000000
VERSION     DAXFHF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.070"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            129..619
                     /locus_tag="ACFIRM_001759"
                     /pseudo
     CDS             129..619
                     /locus_tag="ACFIRM_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            656..922
                     /locus_tag="ACFIRM_001760"
                     /pseudo
     CDS             656..922
                     /locus_tag="ACFIRM_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1424..2047
                     /locus_tag="ACFIRM_001761"
     CDS             1424..2047
                     /locus_tag="ACFIRM_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3029336.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2159..3541
                     /locus_tag="ACFIRM_001762"
     CDS             2159..3541
                     /locus_tag="ACFIRM_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3029337.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKCGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3545..4069
                     /gene="ssb"
                     /locus_tag="ACFIRM_001763"
     CDS             3545..4069
                     /gene="ssb"
                     /locus_tag="ACFIRM_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3029338.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4442..5365
                     /locus_tag="ACFIRM_001764"
     CDS             4442..5365
                     /locus_tag="ACFIRM_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3029339.1"
                     /translation="MENQRPLLGFALALLAVMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFIRM_001765"
     CDS             complement(5430..6818)
                     /gene="fumC"
                     /locus_tag="ACFIRM_001765"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC3029340.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFIRM_001766"
     CDS             complement(6962..8941)
                     /gene="tkt"
                     /locus_tag="ACFIRM_001766"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC3029341.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1861 a         2307 c         2777 g         2256 t
ORIGIN      
        1 gcaagcgtag gtcggatact tgtatccgac aaaaccttta acattcccat cattgcaatc
       61 cattgcagca atgtccaaaa tgtcgaattc aagaatccgg cctacaaaat cattccgagc
      121 ataatactat gaaataccgt cgtttttacc gcaatggcgg cacttacttt tttacggttg
      181 taaccaataa acggcagaag attttgaccg atgatgcggt gcgtttggct ttacggcagg
      241 cggtaatggc ggtgcgcgaa cggtatccgt ttgaaatttt ggcatgggtg ttgatgcccg
      301 atcatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg gaacgctggc
      361 ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt tggcaaaacg
      421 cttttgggaa catactatcc gcggtgaggc cgattttgcc tgccattttg attatctgca
      481 tttcaatccg gtcaaacatg gctatgtagg acaaatttcc gattggcggt tttctacgtt
      541 tcaccgttat gtcaaacagg gtatttatcc gcataattgg ggtggcggca atgcggactt
      601 ttctattgaa tacgattgaa gtaatgtcgg attcgagaat ccgacctacg gaaaactgaa
      661 agagcatcgg ctgctggacg gcattatgcg caaagcccgc cgcaaccgcc cgctgccgga
      721 ggcgcaaacc aaacgcaacc gatatttgtc gaagacccgt tatgtggtcg aacaaagctt
      781 cggtacgctg caccgtaaat tccgctatgc ccgggcggcc tatttcggac tgattaaagt
      841 gagtgcgcaa agccacctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct
      901 aagtgcgccc gctgccgcct aaaaggcggc ccggatgcct gattatcggg tattcaaggg
      961 ggattaaggg ggtatttggg tagagttagg agtgattggt ggcggaaaca gccgaaaatc
     1021 tgtgtcaggg tttcagctgt cgggagggaa gggaattttg caaaggtctc aagatatagt
     1081 ggattaaatt taaaccggta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     1141 cactatattt ccgcctgctg ccgcccgaaa agcgtaatgc gctgatggtg ctgctgcaca
     1201 atccggacga cggataaccg gtgaaaacgg gaaatgcccg ggcggtgtcg tctgtgcctg
     1261 tttggacaag cattcttgag ggcggtaaaa tcaaggtttg cccgaaaagg gcagggcgat
     1321 atgccgcgct tggattttgc gcgcggtttt tcgcgttgga agcaatcatg tctgtttgaa
     1381 tatttaatgc cgtctgaaac gtccggacgc acccccgttc cttatgaaaa aaccgaccga
     1441 taccctaccc gtcaatctgc aacgccgccg cctgctgtgt gccgccggcg cgctgttgat
     1501 cagcccgctg gcgcacgccg gcgcgcaacg tgaagaaacg cttgccgacg atgtggcttc
     1561 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgttcg acaatccgaa
     1621 agagggcgaa cgttggttgt ccgcgatgtc ggcacgtttg gcaagattcg tccccgacga
     1681 gggggagcgg cgcaggctgc tggtcaatat ccaatacgaa agcagccggg ccggtttgga
     1741 tacgcagatt gtgttggggc tgattgaagt ggaaagcgcg ttccggcagt atgcgataag
     1801 cggggtcggc gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
     1861 accggcgcac aacctgttcg atatccgtac caacctgcgt tacggctgca ccatcctgcg
     1921 ccattaccga aaccttgaaa agggcgacat cgtccgcgca cttgcccgct tcaacggcag
     1981 cctcggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
     2041 gcgttgattt tgaacccgtg ccgcaaccga aatacggcga atcctgtata atccgaaaat
     2101 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aattatttat
     2161 ggcaagagac aaccgcatcc aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc
     2221 cggcgtgtac gcgctgcgta tgctgggtat gttcctcgtg ctgcccgttt tggcaatata
     2281 tgccgcctcg ctgcccggtg cggaagacaa taaaacgctg gtcgggctgg caatgggcat
     2341 ttacgggctg acgcaggcat tgctgcaact gcctttgggt atcgcttccg acaagttcgg
     2401 gcgcaagaaa accatttatg tcgggctggt cgtgtttgcc gccggcagct tccttgccgc
     2461 cgccgccgat acgctgccca tgctggttgc cgcgcgcgcc atacagggcg cgggggcggt
     2521 cagtgctgcc gtaaccgcgc tgcttgccga cttgacgcgc gacggcgtgc gtacccgcgc
     2581 gatggcgatg atcggtttga gtatcggtct gacgttttcg gtcagcctgg tggttgcgcc
     2641 gatgattgcc gatgtggccg gcgttcgcgg actgtttatg ctgaccggca ttctgaccgt
     2701 catcagcatc ggcgtagtgg cgtggatgac tcctgatccc gaagtttcca agctgcatga
     2761 ggacacgcag gcgcagcctt cgcgcatagg agaagttttg aaaaaccgta ggctgctgac
     2821 gctcgatttc ggcattttcg ccctgcacgc cgcgcaaatg gcattgttta ccgcgctgcc
     2881 tttcgcgatg acccggctcg gtttggaaaa aatccagcat tggaaagtat atctgccctc
     2941 gaccattacg ggcttggtgg tgatggttcc gctgattatc gtcggcgaaa cgcgcaacaa
     3001 gctcaaacag gtttttgttt tgggcatcgt ctgcatcgcg gcggcgcagc ttggtttgct
     3061 gtccggaatg cactcgattt ggctgattac cgcttatctg gttgtttact ttatcggttt
     3121 taatgtgttg gaagcgagcc tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa
     3181 gggtacggcg atgggcgtgt acaacacgat gcagtcgctc ggactgtttg ccggcggcgc
     3241 ggcaggaggc ttgctgtttc aaaaatgcgg ctttgccggc gtgtttgcct tttgcagcat
     3301 attgatgctg ctgtggctgg tgattgccgt tttatcgccc gcgcccaagc ccgtcaaaaa
     3361 cctcagttac cctgtcggcg gcgtgtggca gggcaatcgg gacggtttgc aacgcgccct
     3421 gttgcagttg gaaggcgtgg aagacatcgg tttcagtttc gacgggcaga ccgtctatct
     3481 taaagtgttg cagaagggtt tcgatcaggc tgccgctgaa aaaatcatta caggagttta
     3541 aaaaatgtca ttgaacaaag tcatccttat cggccgcctc ggacgcgatc ccgaagtgcg
     3601 ctatatgccc aacggcgagg cagtgtgtaa tttcagcgtc gccaccagcg aaacttggaa
     3661 cgaccgcaac ggccagcgcg tagagcgcac cgaatggcac aacatcacga tgtaccgcaa
     3721 actggcggaa attgccgggc aatacctcaa aaaaggcggg ctggtttatc tggaaggcag
     3781 aatccaaagc cgcaaatacc aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt
     3841 cgccaatgaa atgaaaatgt tgggcgggcg caatgaaaac agcggcggcg cgccttacga
     3901 tgaaggttac ggtcagagtc aggaggctta ccaacgcccc gctcagcaaa gccggcagcc
     3961 cgctcccgac gcgccgtccc atccccaaga agcacccgcc gcgccgcgcc gccaacccgt
     4021 gccggccgcc gccccggtcg aggacattga cgacgacatc ccgttctgaa ttttacggcc
     4081 ggacatcctc gcggagggaa gtcataaagg acggaaaaac cttaaacctt acggggcaag
     4141 gtttctcttt tttgcatttg ggccggcttg ttgccgcccc ctgcgttggg cgtaacgttg
     4201 cgattgcgcc gttcgggcgt tttgaggcaa gcgatgcctg ccgcactccc ccgctgtttt
     4261 cagacggcat tttaaaattg aggcggtgtt ttcaaatagt gcgaagcaat agtcgggctt
     4321 cagccagttt gtttgaaatc aaagggttgg aatcaaacgc caaacgcttg aaccatcatt
     4381 gcccgatgcg gcaaaccggc atcgtccggc aatacggttg tttaaaaata gggaaatcag
     4441 gatggaaaac caaaggccgc tcctaggctt cgcgttggca cttttggcgg tgatgacgtg
     4501 ggggatgctg ccgattgccg tgcggcaggt attgaagttt gtcgatgcgc cgacgctggt
     4561 gtgggtgcgt tttacagtgg cggcggcggt attgtttgtt ttgctgacat tgggcgggcg
     4621 gctgccgaag cggcgggatt tttcttggca ttcattcagg ctgctgctgc tcggcgtgac
     4681 gggcatttcg gcaaactttg tgctgattgc ccaagggctg cattatattt cgccgaccac
     4741 gacgcaggtt ttgtggcaga tttcgccgtt tacgatgatt gttgtcggcg tgttggtgtt
     4801 taaagaccgg atgactgccg cgcagaaaat cggtttggtt ttgctgcttg tcggtttgct
     4861 tatgtttttt aacgacaaat tcggcgagtt gtcgggtttg ggcgcgtatg cgaagggcgt
     4921 gttgctgtgt gcggcaggca gtatggcctg ggtgtgttat gccgtggcgc aaaagctgct
     4981 gtcggcgcaa ttcggtccgc aacagattct gctgttgatt tatgcggcaa gtgccgccgt
     5041 gttcctgccg tttgccgaac cggcacacat cggaagtttg gacggtacgt tggcatgggt
     5101 ttgttttgcg tattgctgct tgaatacgtt aatcggttac ggctcgttcg gcgaggcgtt
     5161 gaaacattgg gaggcttcca aagtcagcgc ggtaacaacc ttgctccccg tgtttaccgt
     5221 aatattttct ttgctcgggc attatgtgat gcctgatact tttgccgcgc cggatatgaa
     5281 cggtttgggt tatgtcggcg cactggtcgt ggtcgggggt gcggttacgg cggcggtggg
     5341 ggacaggctg ttcaaacgcc gctagttcgc aggcaacgga aaatgccgtc tgaacgaggc
     5401 ttcagacggc attttatttg agggaaggat tagcgcggat gaaccatatc ggcagggacg
     5461 accagttcgt caaactcttc gcccgtcagc aagcccaacc caacggcagt ttcgcgcagc
     5521 gatttatcgt ttttgtaggc ggttttggcg actttggcgg cgttttcgta gccgattttg
     5581 cggtttaacg cagtaaccag catcagggaa tggtgcagga aatagtcgat tttttccggt
     5641 acgggttcga tgccggcggc gcagttttcg ttgaagctgt tgcacgcgtc gcccaagagg
     5701 cggatggatt gcaagaggtt gtaggcgata acgggcatat agacgttcag ctcgaaattg
     5761 cccgacgcac ccgccatacc gatggtaacg tcgttgccga acacttggca gcacaccatc
     5821 gtcatcgctt cgcattgggt cgggttgact ttgcccggca taatggacga acccggttcg
     5881 ttttcgggga ttttgatttc gcccaagccg cagcgcgggc cgcttgccag ccagcggatg
     5941 tcgttggcga ttttgttcag gcttgccgcc agcgttttca atgcgcccga agcggcaacg
     6001 gcggcatcgc gtccgcccaa ggcttcaaat ttgttcggcg cgctgacaaa cggcaggccg
     6061 gacagttcgg cgagtttggc ggcggctatt tcagcgtatt cgggatggct gttcaaaccc
     6121 gtaccgaccg ccgttccacc caaggcgagt tcgtacaaac ctttgagcgc gtcgttcaga
     6181 cggcctaagc tgtggtcgag ttgggaaacg tagccggaaa attcttgtcc caaagtcagc
     6241 ggcgtcgcat cctgcaagtg ggtacggccg attttgacga tgggggcgaa ggcttgggct
     6301 tttttgtcca atgtatcgcg caaggctttt accgccggaa taaggtggcg gttgatttca
     6361 atcgcggcgg cgacgtggat ggcggtcggg aacgcgtcgt tggtcgattg cgcgtggttc
     6421 acatggtcgt tggggtggac gggccggtac gccgccaaac ccgtacctgc aatttcgttg
     6481 gcgcggtttg ccagcacttc gttcatgttc atattggact gcgtaccgga gccggtctgc
     6541 caaaccacta aggggaactg cccgtcgagc ctgccgttca gtacatcatc cgccgcctgc
     6601 gtaatcaaat ccgcctgttc gggcttaatc ctgccgaggg aaacattggt cgcggcggcg
     6661 gcttttttca ccaacgccaa agcgtaaatc agcggctgcg gcagggtttc gccgccgatt
     6721 ttgaaattgt tgcggctgcg ctgggtctgc gcgccccaat aggcttcgga cgggacttcg
     6781 acattgccca tcgtgtcgtg ttcggtgcgg gtgttcatac gtttctcctt ttgaaatgtg
     6841 aataagagtg attcgcaaat attataatgg agattggcgg aatgaggaag catcaaggcg
     6901 ggagggcaaa aaaatgccgt ctgaaaagca ttcagacggc atttgatgcg gcggctgagg
     6961 tttacagcac ggatttcacc gtatcaacca cattgtccac ggtaaagccg aatgctttga
     7021 acagcagatc ggcaggggcg gattcgccga agcggttgat gccgacgact gcgccgttca
     7081 gtccgacata tttgtaccag ccgttggtgt gtccggcttc tacggcgatg cgcggcaggc
     7141 cttccggtag gacggcggct tgataggcgg cgtcttggcg gtcgaagacg ctggtggacg
     7201 gcatggaaac gacgcgcacg gcaatacctt gtcctgccaa tactttttgc gcttctacag
     7261 ccaatcctac ctcggaaccg gtggcaatga tgacggcttg ggcgttgcct tgggcttcac
     7321 tgattacgta agcaccgcgt ttgatgtcgt tcagttgttg ctcgctgcgt gcttggaatt
     7381 tcaggttttg acggctgaaa atcaggcagg acgggtggtc ttcggctttt gccgcttctg
     7441 cccaagcgac cagagattct gcggtgtcgc acggccgcca tacgtccata ttcggaatca
     7501 ggcgcagggt ggcggtttgc tcgataggct ggtgggttgg gccgtcctcg cccaaaccga
     7561 tggaatcgtg ggtgaatacg aagacggggt tgattttcat cagtgccgcc atgcgtaagg
     7621 cgttgcgttc gtattcgctg aacatcagga aagtcgcgcc gaagggtttt acaccgccgt
     7681 gcaataccaa accgttcata atcgcaccca taccgaactc gcgcacgccg tagtggatgt
     7741 agttgccgcc tttgtcgcgg gtaacggaga cgctgtttga ccagtcggtc aggttggacg
     7801 gggtcaggtc ggcagaaccg cccaccagtt cgggcagctc ttttgccaag atttcgatgc
     7861 tgttttggct ggctttgcgg gtggcgacgg tttccgcttt ggcgcacact tctttcaatg
     7921 ccgtctgaac gtactcgtca aagttttccg gcagcttttt atccatgcgg cgcacgaatt
     7981 ctgcggcttc ggcaggatat ttggcttgat attgcgcgaa cagttcgttc catccggctt
     8041 ccagtttcgc gcctttttct ttggcattcc acgcatcgta aatttcttgc gggatttcaa
     8101 acgcaggata agcccaaccc aaatgtttgc gcgtggcttc gatttcgtcc gcacccaaag
     8161 gcgcgccgtg ggttttgtgg ctgccttctt tgttggcgct gcctttgccg attaaggttt
     8221 tgcagcagat gatggacggc ttgccggttt cggcgcgggc ggcttcgatg gccgtctgaa
     8281 tggcggcggt gtcgtggccg tttacgttgg gaacgacgtg ccagccgtag ctttcaaagc
     8341 gttgcgggat gttttcggta aaccagccgt ccactttgcc gtcgatggag atattgttat
     8401 cgtcatacaa aacaatcagt ttgcccaagc ccaaggtgcc ggcgagtgag caggcttcgt
     8461 gcgatacgcc ttccatcagg cagccgtcgc ccataaagac gtaagtgtag tgatcgacga
     8521 tgttcaaacc gtctttatta aattcggcgg caaggatttt ttctgccaat gccatgccca
     8581 ctgcgttggc aataccttgc cccaacgggc cggtcgtggt ttccacgccg tcggtgtagc
     8641 cgtattcggg atggccgggg gttttgctgt gcagctggcg gaagtttttc agatcttcaa
     8701 tgctcaggtt gtagccggtc aggtgcagca ggctgtacaa cagcatagac gcatgaccgt
     8761 tggagaggac gaagcggtcg cggttgtaga atttgggatt ggcgggattg tgattgagga
     8821 atttcgtcca caatgtttcc gccatttccg ccatacccat aggcgcgccg gggtggccgg
     8881 aattggcttt ttgaacggca tcggccgaga ggaagcggat tacgtttgcc agttgagaca
     8941 ttttgtattt tccttgctgg tgtttcggat aagtggataa tcggaaggcg ttgattatcg
     9001 cccgattcgc ttatgctttc aagaaaaggg cggacgcggg ggaaggcggc ggcagggggt
     9061 gggcggtggg gaaggatttt cgatttgcgg gcgaagcctg ccattattcc ttttgaaata
     9121 aaacgttata gattgtgtgc cggattgttg atagcgtttg tttatgagct tgcgccggcg
     9181 gttctgccga taagggggtg t
//
LOCUS       DAXFHF010000071         9150 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXFHF010000071 DAXFHF010000000
VERSION     DAXFHF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.071"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFIRM_001767"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFIRM_001767"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC3029342.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRSVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PLVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="ACFIRM_001768"
     CDS             complement(2985..3344)
                     /locus_tag="ACFIRM_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029343.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3343..3522
                     /locus_tag="ACFIRM_001769"
     CDS             3343..3522
                     /locus_tag="ACFIRM_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC3029344.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="ACFIRM_001770"
     CDS             complement(3515..4156)
                     /locus_tag="ACFIRM_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC3029345.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="ACFIRM_001771"
     CDS             complement(4146..4697)
                     /locus_tag="ACFIRM_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC3029346.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="ACFIRM_001772"
     CDS             complement(4701..5219)
                     /locus_tag="ACFIRM_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC3029347.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFIRM_001773"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFIRM_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC3029348.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..8207)
                     /locus_tag="ACFIRM_001774"
     CDS             complement(6873..8207)
                     /locus_tag="ACFIRM_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC3029349.1"
                     /translation="MPPFIFVTFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     TDVPQLDKGEAAFCPRCGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVVLYFPANILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIAVARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8518..9147
                     /locus_tag="ACFIRM_001775"
     CDS             8518..9147
                     /locus_tag="ACFIRM_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC3029350.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2041 a         2365 c         2610 g         2134 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgagg ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcattcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgctgcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccatcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttcg taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttgacc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaagcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca aaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccaccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt
     8161 tcggcaaacg gcttcatcgg gaaagtgaca aatataaatg gcggcattat aaaggaaaaa
     8221 aagcgataca cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg
     8281 gtgtccggca aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa
     8341 aaccccgatt ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt
     8401 ttttcgtaaa atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg
     8461 gcaagtccgg attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg
     8521 agcgaaaccg aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg
     8581 gttcacgaca gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac
     8641 tacatcaaat ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa
     8701 gaccccgagt tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta
     8761 acgcaagact tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac
     8821 gaaaccggca acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg
     8881 gcatttttgt tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac
     8941 ctcgctccgc atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat
     9001 gccctgaact taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt
     9061 gctttctata aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc
     9121 gaaggcatga tgccgcacag acattaaaaa
//
LOCUS       DAXFHF010000072         8985 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXFHF010000072 DAXFHF010000000
VERSION     DAXFHF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.072"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(117..773)
                     /locus_tag="ACFIRM_001776"
     CDS             complement(117..773)
                     /locus_tag="ACFIRM_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC3029351.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            838..1728
                     /locus_tag="ACFIRM_001777"
     CDS             838..1728
                     /locus_tag="ACFIRM_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC3029352.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            1845..3656
                     /gene="typA"
                     /locus_tag="ACFIRM_001778"
     CDS             1845..3656
                     /gene="typA"
                     /locus_tag="ACFIRM_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC3029353.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(4053..4143)
                     /locus_tag="ACFIRM_001779"
     tRNA            complement(4053..4143)
                     /locus_tag="ACFIRM_001779"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4107..4109),aa:Ser,seq:gga)
     gene            complement(4182..4268)
                     /locus_tag="ACFIRM_001780"
     tRNA            complement(4182..4268)
                     /locus_tag="ACFIRM_001780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4232..4234),aa:Leu,seq:cag)
     gene            4357..6732
                     /gene="rnr"
                     /locus_tag="ACFIRM_001781"
     CDS             4357..6732
                     /gene="rnr"
                     /locus_tag="ACFIRM_001781"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC3029354.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(7068..8531)
                     /gene="guaB"
                     /locus_tag="ACFIRM_001782"
     CDS             complement(7068..8531)
                     /gene="guaB"
                     /locus_tag="ACFIRM_001782"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC3029355.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTYKVERVLVLNEKDELKGLITIKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(8678..>8985)
                     /locus_tag="ACFIRM_001783"
     CDS             complement(8678..>8985)
                     /locus_tag="ACFIRM_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-P-II uridylyltransferase"
                     /protein_id="HFC3029356.1"
                     /translation="TQSHSRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPF
                     LLADMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
BASE COUNT         2099 a         2290 c         2629 g         1967 t
ORIGIN      
        1 acggttttgg acggaaaaac ggcctgaagc cgtttcgggc attcagaccg tttgtgcggt
       61 ggggaaaatg ccgtcagaaa ggcagaaaag ggcttcagac ggcatgatgt cgggtttcag
      121 gacaggagca ggatggcggc tgcggcaagc gcggcaaccg ataatgcggc ggcaagcgcg
      181 gctttgccgg caaagcggat tgaggttttg ccttcgatgt atttgaagcc ggttatcatc
      241 gggcggacga ggtttttctt tttgaatatg cggtatgcgg cgacggcggc gatgtggact
      301 gcggaaaaaa cggcgagcag cttgaaaaag ttgaggtgga ttttccgtat aaggctgccc
      361 gtatgttcgg aaaccaaatg gttgaggtag ccgttggtgc tgaaggtgtt ttcatcggcg
      421 gcaaaaagcc ccgtgccgac ttgaaatgag acggcggcca aaagcgcaac gaccatcagt
      481 gcgcccaagg ggttgtgtcc gggctggata tgttcgggaa tgccgttttt cagatagccg
      541 cgtatacctg cccaacctcg gacgaaacgg gagaaacggg cggtatcgct gccccaaatg
      601 ccccagcaga ggcggaatac gagcaggaaa aggacgagca gcccgacgcg cgtgtgccat
      661 tgcagcatat cgccgccggc ttttgcgcta taccacataa agggcaggga tgcggcaagc
      721 agccagtgga aaaggcgggt ggggaagtcc cagactttgg ttttgttttt cataatcggt
      781 ttccggcggt agaatcggtt tgttttcgag ccttaatttt aaccgattgg aggggaaatg
      841 tttcccgttt tccatctttc aggcgaaagc cgccgccgga tgcttcagac ggcattgcgt
      901 tttccccatg ttttcaaagc ccgtgcggaa gattcgcaca aagggacttt cggcacgctc
      961 gccgtagtcg gcggatcggc agggatgagc ggcgcgcccg tattggcggc atcggcggca
     1021 atgtatctcg gctgcggtaa ggtgcgggca ggtttcaatc aggatacgct gccttttgcc
     1081 gtcattgccg gttttcccga gattatgttg gacacggcag acggtttgac caagcgtcaa
     1141 ggcataaacg cttggacggc gggttgcggc ttgggtacag atgcggcggc agtggaaacg
     1201 gtggcggcag tgctggcgcg gaatcgagac gaagccgtcg ttttggatgc ggacgcgctg
     1261 aacatattat caaccgatgc cgaaacccga aatctggcgc gcgggtgtaa aaacctgatt
     1321 ttaacgccgc accccgccga agccgcgcgc ctgcttggaa cgacagttgc acaggttcag
     1381 gcggatcgga cggcggcagt aaggaagata ggggcaattc tcggtgcaac cgtggtttta
     1441 aaggggcaca aaacattggt tgccgcgtcc gatacggaaa tctatgtcaa cgaaagcggc
     1501 aatgcgggat tggcaacggc gggcagtggc gacgtattgg gcggcatcat cggcagtctg
     1561 ctcgcacagg gcgtgccggt ttttgaagcc gcctgcgcgg gcgcgtggct gcacggcgcg
     1621 gcggcggatg ttataaagga atcggcaggc attgcggctg ggctgtcggc aggggaaatc
     1681 gccccggcgg caaggtggct gcgcaactgg ataactgaaa gtatgtaaga agatgccgtc
     1741 tgaaaggcaa gggcttcaga cggcatcttc attttccaaa tactgtccgg taaatcgtgg
     1801 tataatacgc agttatctct tacagttttt gaaaaacatt aattatgaaa caaatccgca
     1861 acatcgccat catcgcacac gtcgaccacg gcaaaaccac attggtcgac caactgctgc
     1921 gccaatccgg cacattccgc gccaaccagc aggttgacga gcgcgtgatg gacagcaacg
     1981 accttgaaaa agaacgcggc atcaccatcc tcgccaaaaa caccgccatc gattacgaag
     2041 gctgccacat caatatcgtc gacacgccgg gacacgccga cttcggcggc gaagtggagc
     2101 gcgttttggg gatggtggat tgcgtcgtct tgttggtgga cgcacaggaa ggtccgatgc
     2161 cgcaaacccg tttcgtgacc aaaaaagcct tggctttggg gctgaaaccg attgtcgtca
     2221 tcaacaaaat cgacaaaccg tccgcccgtc cgagctgggt tatcgaccag actttcgagt
     2281 tgttcgacaa cttgggtgcg accgacgagc agttggattt cccgattgtt tacgcttcag
     2341 gtttgagcgg ctttgccaag ctggaagaaa ccgacgagag cagcgatatg cgcccgctgt
     2401 tcgacaccat cctaaaatac acgcctgcac cgagcggcag cgcggacgag ccgctgcaac
     2461 tgcaaatttc ccaactcgac tacgacaact acaccggccg cctcggtatc ggtcgtatct
     2521 tgaacggacg catcaaaccc ggccaaaccg ttgccgtgat gaaccacgag cagcaaatcg
     2581 cccaaggccg catcaaccag cttttgggtt tcaaaggctt ggaacgcgtg ccgcttgaag
     2641 aagccgaagc cggcgacatc gtgattattt ccggtatcga agacatcggc atcggcgtaa
     2701 ccatcaccga caaagacaac cccaaaggcc tgccgatgtt gagcgtggac gaaccgacgc
     2761 tgacgatgga ctttatggta aacaccagcc cgctcgcagg tacagaaggc aaattcgtga
     2821 ccagccgcca aatccgcgac cgcctgcaaa aagaattgct gaccaacgtt gccctgcgcg
     2881 tggaagacac cgccgatgcc gacgtgttcc gcgtatccgg gcgcggcgaa ctgcacctga
     2941 cgattttgct ggaaaatatg cgccgcgaag gctacgaact cgccgtcggc aagccgcgcg
     3001 tcgtgtaccg agacatcgac ggtcaaaaat gcgaacctta tgaaaacctg actgtggacg
     3061 tacccgacga caaccaaggc gcggtaatgg aagaactcgg ccgccgccgt ggcgaactga
     3121 ccaatatgga aagcgacggc aacggacgca cccgcctcga ataccatatt ccagcgcgcg
     3181 gcttgatcgg tttccaaggc gaattcatga ccctgacgcg cggcgtcggg ctgatgagcc
     3241 acgtgttcga cgactacgcg cccgtcaaac ccgatatgcc cggccgccac aacggcgtac
     3301 tggtgtccca agagcagggc gaggcggttg cttacgcctt gtggaatctt gaagaccgcg
     3361 gccgtatgtt cgtatcgccc aacgacaaaa tctacgaagg tatgattatc ggcatccaca
     3421 gccgcgacaa cgatttggtg gtcaacccgc tcaaaggcaa aaaactcacc aatatccgtg
     3481 ccagcggtac cgacgaagcg gtgcgcctga ccacgccgat caaactgacg ctggaaggcg
     3541 cggtcgagtt tatcgacgat gacgagctgg tggaaatcac gccgcaatcc atccgcctgc
     3601 gcaagcgtta cctgagcgaa ttggaacgcc gccgtcattt taaaaagctg gattaatgtt
     3661 taaatgatac gcgatgccgt ctgaaaaatt tcagacggta ttttttattc ggacgggctt
     3721 tgcggcttct tgaagctgtt tcagacggag tttttcctat ccaatcaaga aactgccgcc
     3781 atttttccag cggcatatcg ccccgtcgcg tttcggtatc gggttcggtt tcccggcagc
     3841 atgtcgaaca atattgtttg aagggatacg ccttttgagt ccttacaggc ttaggaagag
     3901 gataaaacat accgcaaaac ataccataaa aaaacggtaa cggatagtaa tcaatggtaa
     3961 cgtagggtaa taggttgtag ctgattttac gcaataaaat caatacgtaa gtaacaaaaa
     4021 atcccaaact gtaaaagttt gggatttgta tttggcagag aggaagggat tcgaaccctc
     4081 gatacgctat tcacgtatac acgctttcca ggcgtgcgac ttaaaccact catccacctc
     4141 tctaatggcg gaaattatcc taatcgggat aatttgttat ttggtgcccg ggagaagact
     4201 cgaacttcca cacccgtgag gataccagca cctgaagctg gcgcgtctac caattccgcc
     4261 acccgggctt tgctttattt gctgttttgc ggtacagtgt cctgccgcaa agaagtcgct
     4321 attatatagt tcataaagag aatgtcaaca gtcaaaatga ataaaaatat taaatcttta
     4381 aatttacggg aaaaagaccc gtttttaagt cgtgaaaaac agcgttatga acatcctttg
     4441 cccagtcggg aatggataat cgaattgttg gagcgcaaag gtgtgccttc aaaaatcgaa
     4501 tcgcttgcac gcgagctgtc gattacggaa gacgagtatg tcttttttga acgccgtctg
     4561 aaggcgatgg cgcgggacgg tcaggtttta atcaaccgcc gaggcgcagt ttgcgcggcg
     4621 gacaagctgg atttggtcaa atgccgcgtc gaggcgcata aggacggttt cggcttcgcc
     4681 gtgccgctca tgccgatgga cgaaggggat ttcgttttat acgaacgcca gatgcgtggt
     4741 gtcatgcacg gcgacaccgt taccgtccgt cctgcgggta tcgaccgcag gggccgccgc
     4801 gaagggacgg ttctggatat tgtcgaacgc gcgcaaagca aagttgtcgg ccgtttctat
     4861 atggataggg gcgtggcgat tttggagccg gaagacaagc gtctgaacca aagcatcgtg
     4921 ttggaaccgg acggcgtggc gcgtttcaaa cccgaatccg gtcaggttat cgtcggcaaa
     4981 attgaggttt atcccgagca aaaccggcct gcagtggcaa aaatcattga agttttgggc
     5041 gattatgccg acagcgggat ggaaatcgaa atcgccgtgc gcaagcatca tttgccgcac
     5101 cgattcagtg aagcgtgtgc caaatccgcg aaaaaaattc ccgaccatgt acgcaaaagc
     5161 gatttgaaag gccgcgtcga tttgtgcgac cttcctttgg taacgataga cggcgaaacg
     5221 gcgcgcgatt tcgacgacgc ggtgtttgcc gaaaaagtcg gacgcaatta ccgcctggtc
     5281 gtggcgattg cggatgtcag ccattatgtc cgccctgacg atgcgattga tgcagatgct
     5341 caagaacgca gtaccagcgt gtatttcccg cgccgtgtga ttccgatgct gccggaaaac
     5401 ctgtccaacg gcatctgctc gctcaatccc gatgtcgagc gtttgtgtat ggtgtgcgat
     5461 atggtcgtta cctatgcggg caatatcaaa gaataccgct tctatcccgc cgtgatgcgc
     5521 tctcatgccc gcctgaccta caaccaagtt tggaaatggc tttcagacgg catcgggaat
     5581 ccgcacaaag cccaaatcga cacgctttac aagctgttta aaattttgca gaaaaaacgt
     5641 ctggcgcgcg gggcggtgga gtttgaaagc gtcgaaaccc agatgatttt cgacgacaac
     5701 ggcaaaatcg aaaaaattgt ccccgtcgtc cgcaacgatg cccacaagct gattgaagaa
     5761 tgtatgctgg cggcgaatgt ttgcgcggcg gattttctgt tgaaaaacaa acatacggct
     5821 ttgttccgca accatttggg ccccaccccc gaaaaactcg ccaccctgcg cgagcagctc
     5881 ggtctgttgg ggcttcaact tggcggcggc gacaacccgt cgccgaaaga ctatgccgcg
     5941 cttgccgaac aattcaaagg caggccggat gccgaattgc tgcaagtcat gatgttgcgc
     6001 tccatgcagc aggcggttta cgaaccgcat tgcgaagggc atttcggttt ggcttatgaa
     6061 gcatacgccc actttacctc gcccatccgc cgctatcccg acctgaccgt ccaccgtgcc
     6121 atcaaagccg tattgaaccg gaaaacctac acgccaaaca aaagctggca ggctttgggc
     6181 gtgcatactt cgttttgcga acgccgtgcc gacgatgctg gccgcgatgt ggaaaactgg
     6241 ctgaaaactt attatatgcg cgataaggtc ggtgaaatat ttgaaggcaa aatctccggg
     6301 gtggcaaatt ttggaatatt tgtcactttg gacgatatcc atatcgacgg tctggtacat
     6361 atcagcgatt tgggcgaaga ttatttcaac ttccgccccg aaatcatggc aatcgaaggc
     6421 gaacgcagcg gcatccgttt caatatgggg gacagggttg ccgtccgggt cgcgcgtgcc
     6481 gatttggatg atggaaaaat cgactttgtc ctaattgccg gagaaagcgg caggcggcgg
     6541 aaggtcaaat tatccgcatc tgccaaaccg gcaggggcgg cggggaaagg gaaatcgaaa
     6601 accaccgccg agaaaaaaac agcccgatgc ggcaaagtaa ggggaagggg cgtgcctgcc
     6661 gttgccgaat cggggaaaaa ggcaaagaaa ccggttccga ttaaggtcaa aaaacggaaa
     6721 ggcaaatcat aatgctgacg gggcggcttg gggagggcgg ggcatacttt tccgaagccg
     6781 ccgcccgatt ttttgatatg gcggccggcg catcatttcc gaccaatcga aacgcccgta
     6841 ttgaaagatt gcgtttattt ccaccgccgt tttaaaggcc gacggtattc ggcagacggg
     6901 gtgcaaacgg cgttcagacg gcattttcat tctttcggcg tgtccgtcag aatggctttg
     6961 cccgtccgcg caatcagccc gtgcggcttg ccttgaacga acaaaaatgc cgtctgaaac
     7021 tcggaaatta ggtttcagac ggcatttgtc ctgaaaggct gttcaaatca gcggtggtaa
     7081 ttcggcgctt ctttggtaat ttgaacgtcg tgaacgtgcg attcgctcat acctgcggaa
     7141 gtgatttcca caaattcggc tttttcgtgc atttcggcaa tattggcgca acccaaataa
     7201 cccatactgg aacgcaaacc gccggtcagt tggtggatga tgttcacaat cgggcctttg
     7261 tagggaacgc ggccttcgat gccttcgggg acgtatttgt cggtgctgtc ggttttgtct
     7321 tggaagtagc ggtcggcaga accttggctc atcgcgccca aggaacccat accgcgatag
     7381 gatttgtacg agcggccttg atagagttcg atttcgcccg gcgcttcttc cgtgccggca
     7441 aacataccgc cgagcatgac gctgtacgcg cctgcggcga gggctttggc gatgtcgccg
     7501 gagaagcgga tgccgccgtc ggcaatcagc ggaacgcccg tgcctttgag ggcttcggca
     7561 acgttgtgaa tggcggtcag ttgcggcaca cccacgcctg ccacgatacg ggtggtgcaa
     7621 atcgaacccg gaccgatacc gactttgacg gcatccgcgc cggcggcaac caaatccaaa
     7681 gcggctttgg cggtggcgat attgccgccg atgacttgga tgtgcggata ggtttctttg
     7741 acccaacgca cgcggtcgat cacgccttgg ctatgaccgt gggcagtatc gacgacaatc
     7801 acgtccgcgc cggcttcgac caaggctttg acgcgctcgt cggtgtcgcc gccggtgccg
     7861 actgccgcac cgacgcgcag acggccttcg gagtctttgt tggcattggg aaactcggtg
     7921 gtttttaaaa tatctttaat ggtaatcaag cctttaagct cgtctttttc gttcaggacc
     7981 aaaacgcgtt cgactttgta cgtgtgcatc agttcgcgcg cttcgtctat gcttgtgcct
     8041 tcggggacgg taaccagacg ttcgcgcggg gtcataatgg cggaaacggg caaatcgacg
     8101 cggttttcaa aacgcaggtc gcggttggtt acgatgccga cgactttgcc gttttcaacg
     8161 acgggcaggc cggacatttt gcgtttgcgc tgcgcgcgca tttccaagac ttcgcggatg
     8221 agcgttgtcg gcgcaacggt tacggggtct ttgaccacgc cgctttcgtg gcgttttact
     8281 ttggaaacgg cgcgcgcctg catttcgggc ggcatgtttt tatggatgat gccgatgccg
     8341 ccttcctgtg ccatcgaaat ggcgaggcgc gcctcggtaa cggtatccat cgcggcggaa
     8401 agcaggggga ggttgagtgt gatttcgcgg gtaagcttgg tttgaagttt aacgtctcgc
     8461 ggcagcacgg tcgaatgtgc gggaaccaac aaaacatcgt cgaaagtata ggctttttct
     8521 acgatacgca taatgctcgg tctttcggtt tgtgcaagat gcacggcatt atagcacgtt
     8581 gccggcggct tgacagttta tattgtttgc aatatccccc gccttcagac ggcatacggc
     8641 gtggcggcgg gatgccgttt gaaaaccggc agggtgctta tgccaactgt tccagcaaag
     8701 cctgtttcaa tgaggactgg atttttgggt ttttcaagtc ggggctgaag acggtaaagc
     8761 tgtcttcgac gcgttcgtcc aacgtggaga ttttggcgta gcgcaggctg acgttgtggg
     8821 cgaagaagac ttccgccatg tcggcgagca ggaaggggcg gttgacggcg gtgatttcga
     8881 cggaataccg gtccggatag tcttcttcgg gggtgatggt gatgcttggc gcaataggca
     8941 tatagcggct gcggcggctg atgcggcggc tgtggctttg ggttt
//
LOCUS       DAXFHF010000073         8977 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXFHF010000073 DAXFHF010000000
VERSION     DAXFHF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.073"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(69..2045)
                     /locus_tag="ACFIRM_001784"
     CDS             complement(69..2045)
                     /locus_tag="ACFIRM_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC3029357.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2191..3030
                     /locus_tag="ACFIRM_001785"
     CDS             2191..3030
                     /locus_tag="ACFIRM_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC3029358.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3229..4260
                     /gene="gap"
                     /locus_tag="ACFIRM_001786"
     CDS             3229..4260
                     /gene="gap"
                     /locus_tag="ACFIRM_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3029359.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4516..5241)
                     /gene="aat"
                     /locus_tag="ACFIRM_001787"
     CDS             complement(4516..5241)
                     /gene="aat"
                     /locus_tag="ACFIRM_001787"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC3029360.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5311..5745)
                     /gene="fur"
                     /locus_tag="ACFIRM_001788"
     CDS             complement(5311..5745)
                     /gene="fur"
                     /locus_tag="ACFIRM_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC3029361.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5972..6349
                     /locus_tag="ACFIRM_001789"
     CDS             5972..6349
                     /locus_tag="ACFIRM_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC3029362.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            6359..7168
                     /gene="dapB"
                     /locus_tag="ACFIRM_001790"
     CDS             6359..7168
                     /gene="dapB"
                     /locus_tag="ACFIRM_001790"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC3029363.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQEVLGLNNR"
     gene            complement(7282..7551)
                     /locus_tag="ACFIRM_001791"
                     /pseudo
     CDS             complement(7282..7551)
                     /locus_tag="ACFIRM_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7642..7800)
                     /locus_tag="ACFIRM_001792"
     CDS             complement(7642..7800)
                     /locus_tag="ACFIRM_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029364.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(7808..>8977)
                     /gene="lpxB"
                     /locus_tag="ACFIRM_001793"
     CDS             complement(7808..>8977)
                     /gene="lpxB"
                     /locus_tag="ACFIRM_001793"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC3029365.1"
                     /translation="WGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARLT
                     GIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVGI
                     DAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYL
                     DAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVFF
                     QTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAADA
                     VLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELL
                     QSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
BASE COUNT         2240 a         2958 c         2036 g         1743 t
ORIGIN      
        1 tgcgatttgc gaaaacgcta ttagaccaaa taaccgggaa tggcgcaagc cttatttcga
       61 catttcattc atatcccctg caagctccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttacccaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ccatatcggt aaacgttttc acataatcgt cattggtggc gcgcgcatat
      541 acgggcatat tgggatgcat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggc tcgcctgcac ttctggctac ctgcacgcgt
      721 gcgatgtcca agtcgagcgc gaaatacgga atatcctctt gggcgaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatgat cagacttgct catcgcttcc
      841 accaacatgc tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caagcgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccaa ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgct tcattttgaa tgcaatggca aacacaacca atgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa gaagccgagc aaaatatcgc ggaacgggcg aatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcaacatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgccagcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaact ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg atacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca gcttgggcaa tgaaaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaga acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatggacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgataaa
     2161 atacgccctt ttgacccgca ccaccgcaca atgaccgcca ccgccgccga tttagaccga
     2221 ctcctccccc aaacccaatg ccgcgaatgc ggctatgaag gctgcctgcc ctacgcccgc
     2281 gcgatgctgc ggggcgaggc gcacaacctc tgcgccccgg gcggcgcaac cgtcgtccgc
     2341 gaccttgccg ccctgctcgg caaacccctt gtcgcacctg ccaaaaccca agccaaagca
     2401 ctcgcccgga tagacgaaac cgcctgtatc ggctgcaccg cctgcatccg cgcctgccct
     2461 gccgatgcca ttatgggcgc gggcaaactt atgcacaccg tcatcaccga cgaatgcacc
     2521 ggctgcggac tctgcgtcgc cccctgcccc gtggactgca tccatatgca gcccgttgcc
     2581 gacaccgtcc tgccgcgcgc gcgccgtttc agcctgtccg acgacagccg ttttgccgcc
     2641 gccgaacacg cgcgtgcgcg ctacctcaaa cgcaacgaac gcaaacagcg cgaagccgac
     2701 gaacgcaagg ccatgcttgc cgaacgcgaa gccgccgtcc gcaacgcgcg tccgcaaacg
     2761 cccgacacac cgaaaaaacc ggcgttcaac cccgccgacc tcatcgccaa agccatggca
     2821 aaagcgcaaa cccaacagga tcgcctcgcc gccgccgaca accgccaagg ctatcaggca
     2881 aaacagatag ccgaagcccg cgaacgcgcc gagttgcgcc gcgcccaacg cgatatgaaa
     2941 tacggcagcg acagcgaaaa agccgccgcc ctcgaatacc tcaaacaata caaagccaaa
     3001 caggaagccg cacagaatac cgcctcctga cccttacccg acatgccgtc tgaagccgct
     3061 tcagacggca ttttatcaag ctctccgtcc gccgtacccg taccgtccgt atcttaccgc
     3121 ccacccttcc ggccgtgccg ttttcaataa aatattaatt acacgccact acaaatttgc
     3181 tataatccgc cccgaaaatc tacccaaccc tcaacaaagg aacaaaccat gggcatcaaa
     3241 gtcgccatca acggctacgg acgcatcggc cgccaggttt tgcgcgccat ctacgactat
     3301 cagattcaag accaactcca aatcgtcgcc gtcaacgcca gcggcagcct tgaaaccaac
     3361 gcccatctga ccaaattcga caccgtacac ggacgctttg aagccgacgt atcccacgac
     3421 ggcggcaacc tcatcgtcaa cggcgacaaa atccccttct tctcgacccg caaccctgcc
     3481 gaactgccgt ggaaagaact cggtgtcgat ttggtcatgg aatgcaccgg cgcgttcacc
     3541 agcaaagaaa aagccaaaat ccacctcgaa agcggcgcga aaaaagtcct catttccgca
     3601 ccgggcggcg acgatgtcga tgcaaccgtc gtgtacggcg tgaacgacag cgtcctgacc
     3661 gccgacatga ccgtcgtttc caacgcttcc tgcaccacca actgcctctc gccggttgcc
     3721 aaagtgttga gcgaaagcgt cggcatcgtc aaaggcgcaa tgaccaccat ccacgcactg
     3781 accaacgacc aaaccgttac cgacgtgcgc cacaaagacc tgcgccgcgc ccgcagcggc
     3841 gtggaaaaca tgattccgac caaaaccggc gcggcaaaag ccgtcggttt ggtcttgccc
     3901 gaattgaaag gcagactcga cgggcttgcc atccgcgtgc cgaccgtcaa cgtatccttg
     3961 gtagatttga gcttccaagc cgcgcgcgac accacagtcg aagaaatcaa cgcactgatg
     4021 aaagccgcct cggaagcagg cgcgctcaaa ggcgttttgg gctacaacac cctgccattg
     4081 gtttccatgg acttcaacca caccaccgaa gccagccact tcgacgcaac gctgaccaaa
     4141 gtcgtcgacg gcaacatggt caaagtgttc gcgtggtacg acaacgaatg gggcttcagc
     4201 tgccaaatgc tgaacaccgc acgccgtatg ttcggacttg aagtgcgccc gctcaaataa
     4261 gcaacaaacc gtcaaacaaa atgccgtctg aaacccgatg ttttcaagtt tcagacggca
     4321 cttttcattt ttgaccgcga ttttacccgg ctgtcgtcat ttccaatttt atagtggatt
     4381 aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     4441 cgccgccttg tcctgattta aatttaatcc actataatcc actatatttg aacctgaccg
     4501 aagcataagc cgccatcaaa gccccttgca cgccacttcg cgccgcccga tttcctcttt
     4561 caacggcacg gcgttcaaca tccgcagacg ttcggcaaaa tccgcaaacg gcagcagctc
     4621 cgaaccgaaa cggcgcatat gttccgtgtc ctgctggcag tctatcagtt ccacgcccaa
     4681 atccgccaaa aacggcacgg cgcaggcaaa cgcgattttc gacgcatccg gttgcaacgc
     4741 aaacatcgat tcgccataaa acaccctgcc gatctgaacg ccgtaaaagc cgcccgccaa
     4801 cctcgtttta ccgttttcat cgggataatg gcactcgaaa gaatgcgcgt gtcccatttc
     4861 gtgcagcttc aaatatgccg tctgaaactc gggcgcaatc caagtcccat cctgattcgg
     4921 gcgcgccgct gccgcacaat gcgcgacaac ttccgcaaaa cagccgttga ccgcaacccg
     4981 atagctgccg ttacgcagcg ttttcgccag cgagcgcgga acgtgcagac gttcgggaac
     5041 aattaccgca cgcggcccga ccgcatacca aaaaaaccaa ccgtcccgcg aaaaccaagg
     5101 aaacacgccg ttccgatacg cctcgagcag ccgccccgcg tccaaatccc tgctcacgcc
     5161 gaccagcccg tcgcaccggg ccaaagcata ggcaggatcg ggaaaggcat aattgtcagg
     5221 ggcaagcagc ggaatacgca tggcagttct ccaaaaacac agacggcatc caaaatcatg
     5281 aatgccgtct gaacaacaaa ccgtccggat ttaacgtttg cccttggcct gacagtcgct
     5341 gcacacgccg tacatataaa gcgcgtgatc gacgatgcgg tagccgtttt cctcggcgat
     5401 tttgtcttgc agggcttcga tttcgggatt gtggaattcc gttacctcgc cgcacttcac
     5461 gcagacgata tggtcgtggt ggtcgccctt gtccaactca taaaccgcct tgcccgtttc
     5521 aaaatggtgg cgttgcagaa tgcccgcctg ctcgaactgc gtcagcacgc ggtaaatcgt
     5581 cgccacaccg atttccacgc cctcttccag cagaatgcgg tacacatctt ccgcactcaa
     5641 atgctcttcc gcgtgcttct cgaacaaatc caaaatcttc aaacgcgggc cggtaacctt
     5701 cagaccgctg tctttcagtt gcgcaatgtt gctgaatttt tccataatat tcaatatccc
     5761 tgtaaaataa tagacgctat aatacgcaat ttcagcctgc ttgcccacta tcacaccata
     5821 gcagttcgca atagcaaaac cgcaacggcg gacgggcagt cggacacaga cagggtaaaa
     5881 taccgcttaa gcgtatgatt atcgttcgct tttataaaat attcaagcag tgctacacta
     5941 gacatcccga tttgcacaga aaggttctcc cgtgaacaaa accctcatcc tcgccctttc
     6001 cgccctgttc agcctgaccg cgtgcagcgt cgaacgcgtc tcgctgtttc cctcctacaa
     6061 actcaaaatc atccaaggca acgaactcga accgcgcgcc gttgccgccc tgcgccccgg
     6121 catgaccaaa gaccaagtcc tgctcctgct cggcagcccc atactgcgcg acgctttcca
     6181 taccgaccgc tgggactata ccttcaacac ctcccgcaac ggcatcatca aagaacgcag
     6241 caacctgacc gtctattttg aaaacggcgt actcgtccgc accgaaggcg acgccctcca
     6301 aaatgccgcc gaagccctcc gcgcgaaaca aaacgcagac aaacaataag gaacacacat
     6361 gacaccgctc aaaatcgcca tcgccggcgc aaacggccgc atgggacgcg tcctcgtcga
     6421 agccgtcaac aaccatcccg acaccgtcct ttccggcgcg ctcgaacact caggctcaga
     6481 agccctcggg ctggacgcgg gctacgccgt cggactcaaa accggcatcg ccatttcaga
     6541 cgacgttgac gccgttctcg cccaaagcga cgtactcatc gacttcaccc gccccgagcc
     6601 gaccctcaag cacctgcaaa aatgcgttga aaaacaagtc aacatcatca tcggcacaac
     6661 aggcttcgac gatgcgggca aagccgccat ccgcgccgcc gccgaaaaaa caggcatcgt
     6721 tttcgccgcc aacttcagcg tcggcgtcaa cctcaccttc cacatcctcg acaccgtcgc
     6781 ccgcgtcctc aacgaaggct acgacatcga aatcatcgaa ggccaccacc gccacaaagt
     6841 cgatgccccc agcggcacag cattacgaat gggcgaagtc atcgccggcg cgctcggccg
     6901 cgacctcaaa caatgcgccg tttacggccg cgaaggccac actggtccgc gcgatccttc
     6961 caccatcggc tttgccaccg tccgcgcagg cgacatagtc ggcgaccaca ccgccctctt
     7021 cgccaccgac ggcgaacgcg tggaaatcac ccacaaagcc ggcagccgca tgacctttgc
     7081 cgccggtgcc gtccgcgccg cagtttgggt aaacggcaaa acgggtttgt acgatatgca
     7141 ggaagtactc gggctgaaca accgttaacc cccatacaaa atgccgtctg aaaagatatt
     7201 gttcagacgg cattttgccg acaggcttcg tatcggcata tcaataaagt tgcttgtgca
     7261 gcatgacaag ctacggctcg tttaattgaa actctcctga tctaaaaatt ccaactctat
     7321 ttcccggcaa actatatcta tgataaaact ggtcggaaga ttatacctac gccttattaa
     7381 tgccagtgca cctccataat ccaagatttc atcatcctgc tttaattctc caagctccaa
     7441 ggtattatcg atatctttat cttggttctc cgtagcgtat ccatagataa atttcagata
     7501 gctgaactgc ttgaatttaa tttttaccca gtctgtgtaa tttggtgtag ggcacaattg
     7561 cctaaaatag aaatttacga agaacaaatg aaacaaggta cgtaagcgta ttagtataaa
     7621 ctataatcat atataataaa attaatcagt taacgaacca ttatatttta accttaatcc
     7681 cattcgggcc agttctgttt catttggaat tcctcttaaa tacttattgt ttgatgtacc
     7741 attaatcaca atttctcccc ctttttttgt aatacgtgct gcttcgggaa aataatccat
     7801 taaccgctca acatcccgcc tcttccaaaa ccgcacgcgc ggccaaatcc gccgtatctt
     7861 ttttcaacag caggtgcaac gccccgaaat cctgttgcag cgcggcaacc ttatcggggt
     7921 gttcgtacca gtccgccaat gccgccgcca gtttttccgg ttttgcttca gattgcaata
     7981 attccggcac ggcctcctta cccaacagga tattcggcag gccgacatgc ggcactttga
     8041 ttttgcgttt cacataagca taggtcagcg gcgaaatctt gtagctgatg accatcggac
     8101 gcttgcacaa cgccacctcc aaagtcgccg taccgctcgt taccagcacc gcatccgccg
     8161 ccctgcacac cgtttcagac tgtctgtcgg ttaccgtcag cgccaatccg gcaaactccg
     8221 gccgctgcaa aacttccgcc aaacgccgct tcgtcgcctc cgttgcggca ggcagcagga
     8281 agcgggcggc gggatagcgt tccaacaaca ataatgccgt ctgaaaaaac accggcgcca
     8341 tatagtcgat ttcgctgacg cggctgccgg gcagcagggc gaatacgggg atgccgacat
     8401 ccgcgcccaa agttttccgc gccgtttcac ggtcgtcttc caagggcata agctgtgcca
     8461 tcggatgacc gacaaactcc gcacgtccgc ccgcatcgag ataaagctgc ggctccatcg
     8521 ggaacaggca cagcacgcgg ttgacctgat gcacgatttt gcccacgcgt tcgcgccgcc
     8581 acgcccaaac cgacggactg acataatgca gggtcggaat gcccgcccgt ttcagctttt
     8641 ccgccacacc cagattaaaa tcgggcgcat cgataccgac aaagacatca ggtttcaacg
     8701 acagcaaatc ccgtaccagc tccctgcgta tccgtaaaat ttccggcagc cgcctgacca
     8761 cttcgacaaa gccgcgcacc gccagccgct cctgatcata aaggctctcg aaaccttccg
     8821 ccttcatcag ttccccgccg atgccggtta accgtgcctg cgggcaacgc tttcggatgg
     8881 cgcgtatcag gtgcgccccc aacaggtcgc ccgacgcttc gccgacactg acggcaatca
     8941 aagggctttt tttatcaacc atattcgtct gtcccca
//
LOCUS       DAXFHF010000074         8694 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXFHF010000074 DAXFHF010000000
VERSION     DAXFHF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.074"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..258)
                     /locus_tag="ACFIRM_001794"
     CDS             complement(<1..258)
                     /locus_tag="ACFIRM_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029366.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFIRM_001795"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFIRM_001795"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC3029367.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSITAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACFIRM_001796"
     CDS             3148..4278
                     /locus_tag="ACFIRM_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3029368.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACFIRM_001797"
     CDS             complement(4319..5221)
                     /locus_tag="ACFIRM_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3029369.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="ACFIRM_001798"
     CDS             5360..5857
                     /locus_tag="ACFIRM_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC3029370.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFIRM_001799"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFIRM_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC3029371.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7285
                     /locus_tag="ACFIRM_001800"
     CDS             6953..7285
                     /locus_tag="ACFIRM_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3029372.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAKAGATDSEIAGALATAIALNAGAAYTYALRA
                     LEAVETQK"
     gene            7416..7886
                     /locus_tag="ACFIRM_001801"
     CDS             7416..7886
                     /locus_tag="ACFIRM_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029373.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7992..8555
                     /gene="pgsA"
                     /locus_tag="ACFIRM_001802"
     CDS             7992..8555
                     /gene="pgsA"
                     /locus_tag="ACFIRM_001802"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC3029374.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVATVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT         2050 a         2147 c         2324 g         2173 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggccaa gatggcttcg ttgactcggc gttctacttc ggcgatttct tcggcagtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaatg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagcggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcggat cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggtaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaac acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggcat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccaaagc cggtgcgacc gacagcgaga tcgcaggtgc
     7201 attggcaacc gccattgccc tgaatgcggg tgctgcttac acttacgccc tgcgtgcatt
     7261 ggaagcggtt gaaacgcaaa aataatccgt ttcggataag aaatgccgtc tgaaaatatt
     7321 tcagacggca ttttgcctgt tatgcttccc gatattttgc agacaacata tgcagccgtt
     7381 tttttcagac ggcatttgag aaacatggaa caagtatgaa atatcaccgc ttggcattgt
     7441 ttgccgccat aagctgcctg ttgttgtccg ccgtttttat tgcaccttac ctgacggcat
     7501 ttcacgaaca agaaaaaata tttgaatatg cggatttgac cgttaccgcc cccaaccgca
     7561 gcggacgggc aatcaaattg gaggcggacg ggcggcagta ccggctttcc tgctacgggt
     7621 tcgacagttt gtgcacaggc ggcaatatcg gcagagccat cagggcgcgg caggttaaga
     7681 ttgttttaag cgaaaccgtc ggcaaaggtt ttttaaacgg cgttctgttg gaataccgca
     7741 acagtggcag tgtctatagc aataaagatt tttcccgcac ggaagaccgc cttgtcgaag
     7801 tgttggcaca acctgccgtt ttcagcctga aaccgggtat tttgctttta ttgcccgcca
     7861 ttttcctgcg tttgaaaaaa atgtgaaaac agattggcgg ggcgggggaa tgactatgcg
     7921 gcaattttac gttttgggtt atgaggataa ccgttataat cataatttta cagtccgcac
     7981 agagaaaacc gatgccttgg aacatcccca tcttcctcac atggttgagg gtcttgctca
     8041 ttcccgtact gattgtcctt ttctacctgc cgttctcatg gttttcagaa gaagcagtca
     8101 atgttgccgc cgccgtcatt tttgccgttg ccgccttgac cgactggttt gacggatttt
     8161 tggcaaggtt gtggaaacag acttccgatt tcggcgcatt cctcgacccc gtcgccgaca
     8221 agctgatggt tgccgtatcg ctgctgctgc tggtcaaact cgaccggacc tatgttttgt
     8281 tcgcgatgat tatcatcggc agggaaatta ccatttccgc attgcgcgaa tggatggcgc
     8341 aaatgggaaa aaggagcagc gttgccgtcg ccaccgtcgg caagttcaaa accgccgcgc
     8401 aaatgctggc gattttcttt ttgctgctga attttcccga tttttacgga tttaatcttg
     8461 ttgttatcgg caacatattg atgtttatcg catctttgct gacagtctgg tcgatgctgt
     8521 attatctgaa aatggcgtgg aaagaaattg cctgaaaaaa acataaaaat agcttgacgg
     8581 taaaaacata atccataata attgcgtctt cttcgatgtc ggagagtgaa atcgggcggg
     8641 aatagctcag ttggtagagc gcaaccttgc caaggttgag gtcgcgagtt cgag
//
LOCUS       DAXFHF010000075         8586 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXFHF010000075 DAXFHF010000000
VERSION     DAXFHF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.075"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..687)
                     /locus_tag="ACFIRM_001803"
     CDS             complement(<1..687)
                     /locus_tag="ACFIRM_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3029375.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNT"
     gene            837..1211
                     /locus_tag="ACFIRM_001804"
     CDS             837..1211
                     /locus_tag="ACFIRM_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC3029376.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            1260..2798
                     /locus_tag="ACFIRM_001805"
     CDS             1260..2798
                     /locus_tag="ACFIRM_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC3029377.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            2865..3482
                     /gene="recR"
                     /locus_tag="ACFIRM_001806"
     CDS             2865..3482
                     /gene="recR"
                     /locus_tag="ACFIRM_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC3029378.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(3538..3816)
                     /locus_tag="ACFIRM_001807"
     CDS             complement(3538..3816)
                     /locus_tag="ACFIRM_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029379.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            4042..5289
                     /locus_tag="ACFIRM_001808"
     CDS             4042..5289
                     /locus_tag="ACFIRM_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC3029380.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            5282..5977
                     /gene="lolD"
                     /locus_tag="ACFIRM_001809"
     CDS             5282..5977
                     /gene="lolD"
                     /locus_tag="ACFIRM_001809"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC3029381.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            6044..7789
                     /gene="recD"
                     /locus_tag="ACFIRM_001810"
     CDS             6044..7789
                     /gene="recD"
                     /locus_tag="ACFIRM_001810"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC3029382.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(7861..8507)
                     /locus_tag="ACFIRM_001811"
                     /pseudo
     CDS             complement(7861..8507)
                     /locus_tag="ACFIRM_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            8476..>8586
                     /locus_tag="ACFIRM_001812"
     CDS             8476..>8586
                     /locus_tag="ACFIRM_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC3029383.1"
                     /translation="MLPPNCGTATPYILRNTVGLDISKLTFDATAIVGNAE"
BASE COUNT         2043 a         2243 c         2448 g         1852 t
ORIGIN      
        1 ggtgttgtag tccaaatccg ccatgtgggt gcaaacttcg ttcaagaagt ggcggtcgtg
       61 cgagatgatg atcatggtgg agtcgtattg gttcaacacg ccttccagcc agcggatggt
      121 attgatgtcc aagttgttgg tcggttcgtc caagagcaat acatccggct tggagaacag
      181 cgcctgcgcc agcaatacgc gcagtttgaa gcccggggcg acttccgcca ttttcgcatt
      241 gtgcaaatct tcggaaatgc ccacgccgct taataattcg gcggcgcgcg cttcggcggt
      301 gtagccgtcg tattcggcga acttggcttc cagttcggcg gctttcatgt agtcgtcttc
      361 ggtggcttcg ggattggcgt aaatcgcatc gcgctcggtc atcgccgccc acatttcggt
      421 atgccccatc atcaccacgt ccagcacgcg catgtcttcg taggcaaact ggtcttggcg
      481 caatttaccc aaacgcacgc cgttttcaat cgccacttcg ccggcggtct gttccaaatc
      541 gccgccgagg attttcatga aggtggattt gcccgaaccg ttcgcgccga tcaagccgta
      601 gcggttgcct tcgccgaatt ttacggatac gttttcaaac agcggcttcg cgccgaactg
      661 catggtaatg ccgttggtag aaatcattat gggtaaaacc tttattgaac aagagattaa
      721 atttttcgaa ttttcggatt gtaacataac cgccccgccg tctgaaacag acgggcaaat
      781 tttcagacac ttatagtaaa atatcggttt tattatttca ggcaaaaggt ttccaaatga
      841 taatccttca cggcattcca aattgcgaca cagtcaaaaa agccaaaaac aggcttgccg
      901 gatacggctt ggagtttggg tttcgggatt tcaaaaaaca gatgccgtct gaagcggaaa
      961 tctgctcgtg gctggaacaa gtgcctttgg caaccctgtt caacaaacgc gggacaagct
     1021 ggcgcaaact cgatgccgaa acacagcaaa aagccctgtc ctcgacggcg gaggccgtca
     1081 aactgatgtc cgaaatgccg agcctgatca agcgtcccgt attggagtgc ggcagcaggg
     1141 tttacgtcgg cttcagcgaa gaaacctacg acggcatctt caaccggcaa gccccgtgca
     1201 gacagggata aaaaccgtta caatacccga cttgaatttc ccattcccaa tctgaaaata
     1261 tgttccattc catcgaaaaa tacagaacac ccgcccaagt cttattaggc ctgattgcat
     1321 taacttttgt cggcttcggc gtcagcacgg tttcccatcc gggcgccgac tacatcgtcc
     1381 aagtgggcga cgaaaaaatc agcgagcact caatcaacaa cgccatgcag aacgagcagg
     1441 cggacggcgg cagcccttgg cgcgacgcgg tgttccaatc cctgctgcaa cgcgcctacc
     1501 tgaaacaggg cgcgaagctg atgggcattt cggtttcttc cgaacaaatc aagcagatga
     1561 ttgtggacga tcccaatttc cacgacgcaa acggcaaatt cagtcacgcg cttttgagtc
     1621 aatacctgtc gcaacgccat atgtctgaag accagtttgt cgaagaaatc cgcgatcagt
     1681 ttgccttgca gaatttggta agcctcgtcc aaaacggcgt attggtcggc gacgcgcagg
     1741 cggaacagct gatcaggctg acgcaggtca accgcaccat ccgttcgcac actttcaacc
     1801 ccgacgagtt catcgcccaa gtcaaagcgt ctgaagccga tttgcagaaa ttttataatg
     1861 cgaacaaaaa agactatctg ctgccgcagg cggtcaaatt ggaatatgtc gccttgaatc
     1921 tgaaggattt tgcagacaag cagaccgtca gtgaaacgga agtgaaaaat gcgtttgaag
     1981 agcgcgtggc gcgtttgccg gcacatgaag ccaaaccttc tttcgagcag gaaaaagccg
     2041 ccgtcgaaaa cgaattgaaa atgaaaaagg cggttgccga cttcaacaag gcaaaagaaa
     2101 agctgggcga cgatgcgttc aatcatccct cctcgcttgc cgaagccgcc aaaaacagcg
     2161 gtttgaaagt ggaaacccaa gaaacttggc tgagcaggca ggacgcacaa atgtccggca
     2221 tgcccgaaaa cctaatcaat gccgtattca gcgacgacgt attgaagaaa aaacacaatt
     2281 ccgaagtgct gaccatcaac agcgaaaccg cgtgggtcgt ccgcgccaaa gaagtccgcg
     2341 aagaaaaaaa cctactgttt gaagaagcca aagatgcggt gcgtcaggcc tatatccgta
     2401 ccgaagccgc caaacttgcc gaaaacaagg caaaagaagt gcttacccaa ctgaacggcg
     2461 gcaaggcagt tgacgtgaaa tggtcggaag tgtccgtttt gggcgcgcag caggcaaggc
     2521 agtccatgcc gcccgaggct tatgcggaac tgctgaaagc aaaaccggca aacggcaaac
     2581 ccgcctatgt cagactgacc ggtctgccgg cacccgtgat tgtcgaggtg caggcagtca
     2641 cgcctccgga ggatattgcc gcacagcttc ctcctgcgaa acaggctttg gcgcaacagc
     2701 agtctgccaa tactttcgac ctgctgatcc gctatttcaa cggaaaaatc aaacagacta
     2761 aaggagcaca atcggttgac aacggcgatg gtcagtaatt gacacttttg ttgacaacat
     2821 aacggtcgga atattccgac cgttttccca tatggcggag tatgatgaat tccaaaaaac
     2881 aggatgcatt ccaacgcctg atcggtgcgc tgaaagtatt gcccaacgtc gggccgaaat
     2941 cggcacagcg gatggcgtac catctgttgc agcaaaagcg caaagaggct gaagagctgg
     3001 tggatgcctt acagacggca ttgaggcagg ttcgccattg cgcaaggtgc aatacatttt
     3061 gcgaaggcgg attgtgcgat atttgtgccg atgaaacacg cgacgggcgg cggctgatgg
     3121 tggtgcatat gcctgccgac gtgtcgaata tagaagcggc aaactgccac gacgggctgt
     3181 atttcgtcct gatggggcaa atcaatacgg cattgggaat ggacgtatcc gccatcgcat
     3241 tggacaggct ggcgcaacgg ctggacggcg gggaaatcga agaaatcatt atcgcgaccg
     3301 cctttaccgc agaaggcaat gccacagcgt atgtcctgtc cgagtttttc aaaaacctgc
     3361 cttacaaggt cagcaggctg tcgcagggca tcccattggg cggcgaattg gaatatgtcg
     3421 atgcgggaac gctggcgcag gcggtgtacg aacggcgtct gattaaagaa ggcggggcgt
     3481 aacgccgcca atgcaaaatg ccgtctgaag ccttcagacg gcattttttc gggcggttta
     3541 cttgcagttg agcccttgaa tctgcaaagt gcccccgctg ccgaccgtaa agacgcactg
     3601 cgccgcgctg ctgccggaaa cggtaatgct gccgctgccg ccgttcaggc tgacaggttc
     3661 cttaacgccc ataccgactg ccgttgccgc cagcttttgc gcgtcggctt cggaataagg
     3721 cgtgttgttg gcatcgattt tgatttcttt ggcataggct gtcgaaacag taaaaacggc
     3781 aacagcacac aaagcggaga ttttcctgat attcattgtt ttccttaaag aaaaggtttt
     3841 gtttcaagag aaggataggg tagttcggcg cgttgtgcaa gttttccgtc ctgtttttcc
     3901 gaatttgcca cataccgcgt ctttatttac acagcctgcc gttcggacgc tgccggggtt
     3961 ttacggtaca ataaactttt gccgcctccc ccaaaccgcc gtccggggcg gcacgtttgc
     4021 agacattttt aaggtagcgt tatgttttct ctagaggctt ggataggctt gaggtatctc
     4081 agggcgaaaa agcgcaacgg ctttatgtcg tttatcacga tggtttcgat tgccggaatc
     4141 gccttgggcg taaccgcgct gattgtcgtc ttgtcggtta tgaacggctt tcagaaagaa
     4201 atacgcgggc agctcctgaa tgtcgcgccg cacgccgaaa tcggctatat cgacaatacg
     4261 gatacggatt ggcgcaacct gcttcggttt gccgaaaacc gcaaaggcat tttggctgcc
     4321 gcaccctatg tttccaatca ggcattgctg gcaaacgcgg gcgaaatcag gggcgttcag
     4381 atacgcggca ttttgccgtc tgaagagcgc aaagtggtgg aatacggcga caaaatgccg
     4441 gcaggcaagt ttgaagacct gattccgggc gagtttgaca ttatcctcgg tgtcggtttg
     4501 gcggaggctt tgggggcgga agtcggcggc aaggttacgg taattacgcc ggagggcaac
     4561 gttacccctg ccggagtcgt gccgcgcctg aagcagttta ccgtggtcgg cctggtcaag
     4621 acgggtgttt acgaagtgga caactcattg gcgatgacgc atatccaaga cgcgcgcgta
     4681 ctgtaccgct tggataagga agttgcgggg ctgcggctga agcttgccga tccgcaaaac
     4741 gcccccgcct tgacggcaac gctgattccg gaggcgcaaa gggacacggt ttgggtgcgc
     4801 gattggacgt acagcaaccg cagctatttt gaagcggtcg aactggaaaa acggatgatg
     4861 ttcattatcc tgacgctgat tatcgccgtg gcggcgttca accttgtctc ttccttggtg
     4921 atggcggtta cggaaaagca ggcggacatt gcgattttgc ggactttggg tctttcccct
     4981 gccggcgtga tgaagatttt tatggtgcag ggcgcgtttt caggcttttt cggcacgctg
     5041 gcgggtgtgg tctgcggcgt gctttggggt tggaacgtcg gcagggtcgt ggcgtttttt
     5101 gaaaacctgc tcggtgtcca cctcatcaat tcgcaggttt attttatcga ctacctgccc
     5161 agtgatgtcg atatgggcga cgttgccctg attgcctgca tttccttggg gctgtctttc
     5221 gtggcgacac tgtacccgag ctggcgcgcg tcaaaaaccc aaccggcgga ggctttgcgt
     5281 tatgagtgat ttgattttga aatgcgaagg cgtgggcaaa cgctaccgtg acggcggttt
     5341 ggacgttccg gtgctgtacg gcttggattt ggaaatccgc acgggggaaa gcaccggcat
     5401 catcggctct tcgggcagcg gcaaatcgac gctgctgcat attttgggcg ggctggatat
     5461 gccgtctgaa ggcagggtgc tgctgatggg cgaggatttg cgtgccttaa accagcggcg
     5521 tttgggcgat ttgcgcaacc gccatctcgg tttcgtgtac cagttccatc atcttctgcc
     5581 tgaattttcg gcactggaaa acgtgatgat gccgcttctg atcggcaaaa aaagccgtaa
     5641 agaggcggta gaaacggcaa tggcgatgct cgacaaggtc ggactgaaac accgttcgac
     5701 gcaccgtgcg ggcgaactgt ccggcggcga acgccagcgt gccgccattg cgcgcgcctt
     5761 ggttacgcaa cccaaatgcc tgcttgccga cgaaccgacc ggtaacctcg accgggcaaa
     5821 cgcaaggaat gttttggata tgatgctgga actgaaaacg gaattgggaa cggggctggt
     5881 ggtcgtaacg cacgacgacg aactcgccgg ccgcttcgag cgcgtgatgg tcatgaaaga
     5941 cggcagcctg caccccagac agggcgcaaa cgcctaaaca gccgaaacaa cacgcaccgt
     6001 ctgaagcccc gtgccttcag acggcatttc gacaaggaat acgatggaac tgcagacaga
     6061 tgaatttgcc caagcggcgg caagggcggc aatccgtttt ttggaacgct atgcgggttc
     6121 gggcaacgaa gtgcttgcaa actgtaccga acgcctgttt caggcattgc aaaacggtca
     6181 ttcgtttatc cgtttgagcg gggacgaggc tgacgcgctg tcggcactcg cacccgtggt
     6241 cggaacatcc gccgcgcctt tgattttgga aggcagaagg ctgtttttgg gcaggatgtg
     6301 gcagttggaa tacgatttgg ctgccgaaat aaaacgcttg gcggcagccg gcacgtccgc
     6361 ccccgacgcg gcaggcgcgc ggcaaaacct cgcaaaatgg tttcaaggcg cgggaagcga
     6421 aggacagcgc gatgcggccg ccttggcact gttgcagttt tttatggtga ttaccggagg
     6481 gccgggaacg ggcaaaacga caacggttgc caaactgctg gcactgattt gcggtgaaaa
     6541 cgaaaatctt ccccatatcg cgcttgccgc accgacgggc aaagcggcgg cacatatggc
     6601 gcgcgcactg caccgtgcaa tcaacggttt tgacgcgccg gaggccgtcc gccgccattt
     6661 gctcaaactg gaagggcaaa ccgtccaccg actgcttaag ctgagcccgc ccaaaatgca
     6721 ggcggcgttc gaccatatcc gtccgctgcc gttcgacgtg ctgattgtcg atgaagcctc
     6781 catgttggat accgcattga tgctgcaact tttgaaagcg gtcaaaaccg gcgcgcgcgt
     6841 gattctgctg ggcgatgaaa accagctccc gtccgtcgga atcggggcgg tgctgtccgt
     6901 tttgtcgcaa aaaaccgttt tggacggaga aacgcaccaa aggctggccg gtttccttcc
     6961 ggaacacggt ttcagcgtca gcgcaaatcc gcccgtgttg gcgcaaaaca ccgcccatct
     7021 gtcgttcagc caccgcttcg gcgacaacag cggcatcggc tgccttgccc gtgccgccgt
     7081 atcgggcgat gaaggggcgt gggcattgtt tgaccggttt ccggacgaac tggaacattc
     7141 ggaatgcagt ccgaatgctc gggtcgaaag gttgtaccgg gcgcacaaag cctattggca
     7201 ggcggtaaaa gacggcaata tcgaagccgc atacgcaggt atctcggata tcgtggttct
     7261 ggcagcttgg cggcaggatg cggaagactt caacgaagcc tattgcagct atgtacgccg
     7321 caaaatgaac ataccggaac atttggccta tttcgccgga gagccgatta tgatcagaca
     7381 gaacgactac gcgcttgagc tgttcaacgg cgacatcgga ctgattatgg aagatgtcgg
     7441 acggcagggc agccttgccg cctattttgc cgatgcggac ggatttaaaa aggtagcggt
     7501 aagctgcctg cccgaatttg aacccgcatt cgccatgacc gtccacaaaa gccaaggttc
     7561 ggaatatcgg gaagtctggc tgctgccgcc ttccgacgca ccttcggacg aaggggacga
     7621 tgcattgtcc ggattgagta aggagctgtt atataccgct attacccgcg ccagggaaaa
     7681 attcgtattt ttcggcggca aaaagacatt ttgtcaggcg gtaaacaccg tcaaagtccg
     7741 tcagacggcg ttgggcagta tgcttgagcg ggtattttca caagaataat ccgcccgaat
     7801 gatgccgtgc cggcggcgcg ggcgttccga tgcctaccgt cggaagcacg ccaccgcccc
     7861 ttatgccttt ttcaaacggc acaggaaagt agtttccctg tttcgggcaa aagccagcgg
     7921 atcgttcgaa tcggcagctt ttttgtgaac cggtttggtt ttcaaaacat cctcaacacg
     7981 caaacccgcc cggataaatg ccttttccaa aaagtcggca gaccgcagcc ccaaatgcac
     8041 ggcgaggtcg gaaatgataa gacggatttc tccgtcggga ttcagatgtt tggcgcatca
     8101 cgcaaaaacg cagccagcat cgcagattcg gggtcgtata acgcggattc gacggcggaa
     8161 gtcggcttgg cgggaagcca gggcggattg cagacaatca ggtcggcaaa cccttcggga
     8221 aataaatcgg tttcgcggat ttcaacctgt ttttcaaaac ccaaacgggc aatattggca
     8281 cgggcgcagg caaccgccct cggattggta tccgtgccga tgacggaagg aatgccctgt
     8341 ttcgccaaaa tggcggcaag tacgcccgag cccgtcccga tatcgaatgc cgtctgaaaa
     8401 cccgttgacg gcgcatgggc aagcaggtcg agatattcgc cgcgcaacgg cgagaatacg
     8461 ccgaaaggaa cgtgtatgct gccgcccaac tgcggaacgg caacccctta tatattgcgt
     8521 aacactgtag gattggacat atccaagctg acatttgacg caacggccat tgtcggcaat
     8581 gccgaa
//
LOCUS       DAXFHF010000076         8352 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXFHF010000076 DAXFHF010000000
VERSION     DAXFHF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.076"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            1..185
                     /locus_tag="ACFIRM_001813"
                     /pseudo
     CDS             1..185
                     /locus_tag="ACFIRM_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
     gene            333..558
                     /locus_tag="ACFIRM_001814"
                     /pseudo
     CDS             333..558
                     /locus_tag="ACFIRM_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            668..1345
                     /locus_tag="ACFIRM_001815"
     CDS             668..1345
                     /locus_tag="ACFIRM_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC3029384.1"
                     /translation="MTFFKPFTVVLTASVLALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1619..2389)
                     /gene="xth"
                     /locus_tag="ACFIRM_001816"
     CDS             complement(1619..2389)
                     /gene="xth"
                     /locus_tag="ACFIRM_001816"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3029385.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIISRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2449..2730)
                     /locus_tag="ACFIRM_001817"
     CDS             complement(2449..2730)
                     /locus_tag="ACFIRM_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3029386.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2983..3234
                     /locus_tag="ACFIRM_001818"
     CDS             2983..3234
                     /locus_tag="ACFIRM_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029387.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDALDRCSG"
     gene            complement(3465..4346)
                     /gene="nadC"
                     /locus_tag="ACFIRM_001819"
     CDS             complement(3465..4346)
                     /gene="nadC"
                     /locus_tag="ACFIRM_001819"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC3029388.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4422..5360)
                     /locus_tag="ACFIRM_001820"
     CDS             complement(4422..5360)
                     /locus_tag="ACFIRM_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029389.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            5562..6674
                     /gene="nadA"
                     /locus_tag="ACFIRM_001821"
     CDS             5562..6674
                     /gene="nadA"
                     /locus_tag="ACFIRM_001821"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC3029390.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6726..8234
                     /gene="nadB"
                     /locus_tag="ACFIRM_001822"
     CDS             6726..8234
                     /gene="nadB"
                     /locus_tag="ACFIRM_001822"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC3029391.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1810 a         2444 c         2352 g         1746 t
ORIGIN      
        1 gcaatgggac atcggcggca gcggggcggt tttcccttcg ctcgcactgt ttctgctctg
       61 tttcatcata ggtatgcaca acacggggat gacgcttctg ccgggcggtg caatccgttc
      121 gacgcacatg gcccggcacg gcagccgact tgggcatcga aatcccgcgc gtgccgtact
      181 atagtggatt aacaaaaacc agtacggcgt tggcccgcct tagctcaaag agaacgattc
      241 tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tctgtactgt ctgcggcttc
      301 gccgccttgt cctgattttt gttaatccac tatattccta aacccaccat ccgcgcctgc
      361 cgcacgtaca cgtcgacaaa ccgaaaatat ggctgctcgg cggcatcgtc ggcgcgtggg
      421 gctgccataa aatcggacac catttctccc tgcccgtttc ggcagtattg ccgataccgg
      481 gtgcaggctc ggtcggctac gatgtgaagg tacgtttttt tgcggatgtg gctgcggttt
      541 cggcgcaaat cacgataaac ccgcgttgtt tcgggaaaaa tcgggtaatt tcaggttaat
      601 tgcacttttt cagacggcat gattgcttat agttgtacgg tttgacatat gcacggaagg
      661 aaacgccatg actttcttca aaccctttac cgtcgtgctg accgcatccg tactcgcgct
      721 ttccggctgc gttgccgacc ccgtaaccgg acagcagtcc ccaaacaaat ccgccatgta
      781 cggtttgggt ggcgcggcag tgtgcggcat cgtcggcgca ctgacccaca gcggcaaagg
      841 cgcacgcaat tccgcgcttg cctgcggcgc aatcggcgca ggcgtgggcg gctatatgga
      901 ctaccaagag cagcgtttgc gccaaaacct tgccggcacg caaatcgaaa tccaacgcca
      961 aggcaaccaa atcaggctgg tgatgcccga aagcgttacc ttcgccaccg gcagcgcggc
     1021 gttgggcggc agtgcgcaat acgccctgaa cactgccgca cagacgctgg tgcagtatcc
     1081 cgacacgacg ctgaccatca acgggcacac cgacaacaca ggttccgatg cagtcaacaa
     1141 tccgctttcg caacaccgcg cccaagcggt tgcctactat ctgcagacgc gcggcgtggc
     1201 ggcttcgcgc ctgacggttt acggctacgg ttcgcatatg ccggtcgcgt ccaacgctac
     1261 ggttgaaggc cgcgcgcaaa accgccgcgt cgaaatcctc atcaaccccg accaacgcgc
     1321 cgtcaacgcc gcacggcaca tgtaacgcgc cctgcaacgc caatgccgtc tgaatgtttt
     1381 tcagacggca tttccgtatt cgcaagcaca gccgtacccc gactgtaatg ctatggataa
     1441 cgtctcgccg tttgccggga aaccgcagat tgcagccggt ggcgtgcaag tgggcccaac
     1501 cttgcagacg ttaccgccat accgcgatac ccctgtaacc tcatcgttta aacagatatt
     1561 caatcaaaat gccgtctgaa accctgcgaa gtttcagacg gcatttgcct ttcccgaatt
     1621 acaaatcaaa ttctgccgcc acgggcgcgt ggtcgctcgg acgctccagc gcgcgcgtct
     1681 ccaaatcgat gcggacatcc ttcaaaacgg ccgccatttc ggatgtaacc aatatatggt
     1741 cgatacgcag gcccagtttg cgttggaaca tcgcgccgcg atagtcgaac caggtataga
     1801 acgcgccttc gggatggact tggcgcaggc tgtcggtcaa tcccaaatcc agcaggtttt
     1861 gaaaccactg ccgttcgacg gacgaacagt ggattttttc ataccatttt tcagggtcgt
     1921 aacagtccgc atcggcaggc gcaatattga aatcgcccag cagtaccagt ttgccgtggc
     1981 gggtcatttc gtcgcggaca aactccgtca gtgcggcaaa ccactgttcc ttatatttga
     2041 atttggggct gtccaaagcc tcgccgttga cgcaatagac attgatgacg cgcacgccgc
     2101 ctacggtcgc cgcaatcacg cggcgttgcg ggtcatccgg cagggaaggc aaaccgaaat
     2161 gcacgtcctg cggcacgctg cggctgatga tcgccacacc gttgtaggtt ttctgcccgc
     2221 tccaaacaca gtgccagccc atcatctgca aggcggcggc cggaaatttg tcctgatcga
     2281 gtttgagttc ctgcaaaacc aaaatatcgg gcggattgtc ggcaagcagg ttttgcacct
     2341 gcggcagacg tacattgagc gaattgacgt tccaagtggt gattttcata acattttcga
     2401 aaaatgccgt ctgaaacgtt cagacggcaa aaggaaagga ttctaacact atgccgcgcg
     2461 tttgttttcc aaatcgcgga ttgtccgcag aatcgttgcc gcgtcttcgg aaaccaggcg
     2521 gtattcgata atgcggtggt aacgcgtaaa gtccaccaga ccgcccgccc tcaggcggtt
     2581 caaatgacag gaaaccgcgg tggccgacag ggataaggat tttgccagtt cgacgatatt
     2641 gcgttcgctg tccaacagtt ggatcagtat cgccatacgt tcgggatgcg ccataagttt
     2701 gagtatggtg tggagcggta ttgtgttcat cggtattcct aaaacctttt ccgcccgcca
     2761 agcggattga cgggcggcag atatggatga ttttgattat ctttataaat tgtattataa
     2821 atcaattgtt tatttctcat tgtacaggat tgtatgttcc ttttcccact atttttgcaa
     2881 acaagcgtaa agacaggctt tcagacggca taaaactgcc gcccctaccc tgccttaccc
     2941 tgttgccgaa cggaatcatc cgtatttttt aagaaaacca ttatggatat tttccgtgaa
     3001 tcaaggccgt tctgcgcgac aggctgtcca acgccaacat ccggttcggc atttcatcat
     3061 cgccgccgac gatacgggca agaccgcatc ggtcgtgcct tcctaacgat gcaccgcatc
     3121 ctcgacaagg aaaaccagat acataccgat atggctttag cgttgccggc ggtagtgaaa
     3181 gacgcagtgg cgatatgggc agatatggat gcattggata ggtgttccgg ctgaccgctc
     3241 cgttactgcc gtatctttcg gctaccgttc atcaaatggt tccaatgccg aaatgcctaa
     3301 atcgcgcctg cctgaaatgc tgttttttct aaagcatcac gaccttcacg aaaaatgccg
     3361 tctgaagaat atttcagacg gcattccctc cctgaggcaa taaccggatt acggttaagc
     3421 ggttccttac ctgacatatc agacagccgc ccgcaccctg aaactcacgc cacgaaatcc
     3481 aaacctatgt ccaacgatcg gctgctgtgg gtcagatagc cgagggcgat gccgtccacg
     3541 ccggtttgcg ccacgcgctt caggcggtcg aagccgatgc cgcccgatgc ttcgcaatag
     3601 acggtgtgtg ggtgggcggt ttgtgtgtgg cagcggtttg ccgcttcttt cagggtttcg
     3661 tcgtccatgt tgtccagcaa aatccgttcc gcgcccgctg cgatggcttc gtccagttgt
     3721 gccaacgtgt ccacttcgat ttccacgcag gtcaacggtc cgactgcctg ttttgcctgc
     3781 cgaacggctt gggcgatgct gccgcaatag gcgaggtggt tgtctttgat gagcacggcg
     3841 tcgtccaaac ccatgcggtg gttcgcgccg ccgcccgccc tgacggcgta tttttgcagg
     3901 acgcgcagca gggggatggt tttgcggctg cacacgatgt ctgtaccgta ttcggcgact
     3961 tcggcaacgg cacgagcggt ggcggtggcg atgccgctta agtgcgtgag gtagttgagc
     4021 gcggtgcgtt cggcggcgag cagcgcgcgg gcgttgcctt cgacggcagc cagtgtctga
     4081 cctgcgcgga cggcttgccc gtcttggatt tcggcttgga agcggacgca cggatccatc
     4141 gtctgaaagg cgaggcgcgc caagtccata ccggcgataa caccgtcttc gcggctgacg
     4201 aggaaaagtt tggcggtttt gtcgggcgcg atgacggcgg cggacgtaat gtcgccgcgc
     4261 ctgcccaagt cttcgctcaa ggcttgttcc accatggggc gcaacagggt gtcgggcagg
     4321 gcaaagaggg ggttttcaga cggcatgggg tttcctttgt taaaaaagat tgatgttgca
     4381 ccgatggggc aaaacttcat ttgatgccgt ctgaaaacgg gctaacggct gcccagcggc
     4441 agtccgatgt ccgaaatcag cctgtcggac aggacgttgt cgcggaagcg gtaaagctgc
     4501 gcgggacgac ctttgctgcc cgatacgccg gtatccgacg gctcgataag gttttgctgc
     4561 tgaatctggc ggcggaagtt ttgcttgtgc agcaatctgc cgctgatggc ttcgacgctg
     4621 ttttgcagtt gcagcagtgt aaatccgggc ggcatcagtt cgaaaatcac ggggcggtat
     4681 ttgattttgg cgcgcaggcg ggacagggcg gtcgccaaca cgcggcggtg gtcgtggcgc
     4741 atggtctgcc ccgtgagcgc gaagtcgaag tttgcctgcg gctcggcggc ctccgctatc
     4801 agaccgcttt catacaacat ttcatagcgt tgcaaaacgt attcttccga ccagttttcc
     4861 ggttcgaccc cccaacacaa atgaatatgc ttgagccgct tttggcggac ttcctccgtg
     4921 tccgccgagt ttgcccaaat gcgcaggcgg ctgacgacgg cgtcgcgctg cccgccgtcg
     4981 gtgcgcaagt cttcccatgg gaaatagccg tagcagtcct gccatttcgc gtcgggatgc
     5041 aggatgctat cggctgcctc gcgcaccagc cccaaatagc tgacgtacag cacgggcatg
     5101 ccgtgttcgt tgcggcggtg ggtatcgaca aaggtgtaaa gctgttccac atagcccata
     5161 ggctgcgaag tctgcttggc gacccacagc tttacgcccg cctgcaggga attgcgcagg
     5221 ggggaaagcg ggccgttggg caggagcgtg ccttgggcga cggtcaacac ccgcaggccg
     5281 ccgtcggtaa cggcaatcaa tacgggaacc agctcgacga tgctttgcag cggagcttcg
     5341 gcttcggggt aggcgtccat gctcggcttt ctgcgggtgg gaaaggcttt attgtaaacc
     5401 aaagcggttt gtcggaacga agcgggtcgg ttttgcggta tcgcaaacct gttttcagac
     5461 gaccttgcat attatactca agttgagcat aatgtaaaaa cagtttgaaa aacagacggc
     5521 aaaccttcat gccgtctgaa aaccagctac aaggaaacat catgcaaacc gccgcccgcc
     5581 gctcgttcga ttacgatatg cccctcatcc aaacgccgac ttccgcctgc caaatccgtc
     5641 aggcgtgggc gaaggttgcc gacacgcccg accacgagac ggcaggtcgt ctgaaagacg
     5701 aaatcaaggc cttgctgaag cggaaaaacg cggtcttggt ggcgcattat tacgtcgatc
     5761 cgctgattca ggatttggct ttggagacgg gcggatgcgt gggcgattcg ctggaaatgg
     5821 cgcgcttcgg cgcggaacac gaagccggta cgctggtggt ggcgggcgtg cgtttcatgg
     5881 gcgagagcgc gaaaatcctc tgccctgaaa aaacggtgct gatgcctgat ttggaagcgg
     5941 aatgttcttt ggatttgggt tgtcccgaag aagcgttttc agcgttttgc gaccaacacc
     6001 ccgaccgcac ggtggcggtg tacgccaata cttccgcagc cgtgaaagcg cgtgccgact
     6061 gggtggtaac gtcttcggtg gcgttggaaa tcgtgtcgta tctgaaatcg cgcggcgaga
     6121 aactgatttg ggggcccgac cgccatctcg gcgactacat ccgccgcgaa acgggcgcgg
     6181 atatgctgtt gtggcagggt tcgtgcatcg tccataacga attcaaaggg caggaactgg
     6241 cggcgttgaa ggcagagcat cccgatgctg tggtgctggt tcatcccgaa tcgccgcaaa
     6301 gcgtcatcga actgggcgac gtggtcggct cgaccagcaa actactcaaa gccgccgtat
     6361 cgcgccctga aaaaaaattc atcgtggcaa ccgatttggg catcctgcac gaaatgcaaa
     6421 agcaggcgcc cgacaaagaa tttatcgccg cgccgacggc gggcaacggc ggaagctgta
     6481 aaagctgcgc gttctgcccg tggatggcga tgaattcgct gggcggcatc aaacacgccc
     6541 tgacaggcgg acgcaacgaa atcctgttgg acagaaagct gggcgaagcc gccaaactgc
     6601 ctttgcagcg tatgctcgac ttcgcggcag gactcaaacg cggggatgtg ttcaacggca
     6661 tggggcccgc ctgatttgcc gtccatatcg ccgtttcaga cgacctctca aacaaggaca
     6721 acaccatgca aaccgattgc gacgtattga ttgccggaaa cgggctggcg gcactgacgc
     6781 tcgccctgtc gctgcctgaa tcgttccgca tcgtcatttt gtgcaaaaac cggctggacg
     6841 acaccgccag ccgtcatgcg caaggcggga ttgcggcggc gtggtcggga gaggacgaca
     6901 tcggaaaaca cgtcgccgat actttggaag cgggcgcagg tttgtgcgac gaagccgccg
     6961 tccgcaccat cctgtcgcag ggcaaaccgg caatcgaatg gctgctggcg cagggcgtgg
     7021 cgttcgaccg gaatcataac gacctgcacc tgacgcgcga aggcgggcat acctgccgcc
     7081 gaatcgccca cgtcgccgac tacacgggcg aagccgtcat gcagagcctg attatccaaa
     7141 tacgccgccg accgaacatc cgcgtttacg agcggcagat ggcgttggac gttcaaaccg
     7201 aatcaggcgc agcgtgcgga ctgaccgtcc tcgactgccg aacgcaagaa acctaccgca
     7261 tccgtgcccg ccataccgta ctcgcaggcg gcggcctcgg gcaaatttac gccgccacca
     7321 ccacgccgcc cgaatgcacg ggcgacgcca tcgccatggc gatacgcgcg ggttgcgcaa
     7381 tcgaaaacct cgaatttatc caattccacc ccacaggctt ggcaaggccg tctgaaaacg
     7441 gccgcacctt cctgatttcc gaagccgtgc gcggcgaagg cggcatcctg accaatcaat
     7501 cgggcgaacg gtttatgccg cattacgacc gccgcgccga actcgcgccg cgcgacatcg
     7561 ttgcccgcgc catcgccgcc gaaaccgcca aacaaacgca agacttcgtc tcgctcgaca
     7621 tcagccgcca acccgcagcg ttcgtccgtc ggcatttccc gtccatccat cggcactgcc
     7681 tgtcccaatg cggcttggac atcacgcgcc aagccatccc cgtccgcccc gtgcaacact
     7741 acacctgcgg cggcatccaa accgacccca gcggcagaac ctccctgccg cagctctacg
     7801 ccttaggcga aaccgcctgt accggactgc acggagccaa ccgcctcgcc agcaactccc
     7861 tgctcgaatg cgtcgttacc gccaggcttg ccgcccaagg catcgcggac ggacaagcgt
     7921 tccaaatcga accattccaa aggccgtctg aaagcccctc cgccgaaaca gacatctttt
     7981 caaacgacct ccaaaacaca ttcagccgcc ctgtcctgca aacgttcaac caacgccatc
     8041 tcggcatcct ccgcaacgat accgacctgc accgcgccat cgaccaactg cggctttgga
     8101 agcaaaacca agccgaaccg cacaccgcgt ccgaatacga aaaccgcaac ctactcgaat
     8161 gcagcctcgc cgtcgcccaa gccgcataca ggcggagaca gaacatcggt gcgcatttta
     8221 atagtgattg ttaaggttgg ctggttagaa aaaaatcgtt caaataaatt tgaatagcat
     8281 ggttaagaga ttggcattca gtttttatgg ttgcgtaagt tgggttgagg cctttgcaaa
     8341 aaagcccttc ct
//
LOCUS       DAXFHF010000077         8098 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXFHF010000077 DAXFHF010000000
VERSION     DAXFHF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.077"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            91..1098
                     /locus_tag="ACFIRM_001823"
     CDS             91..1098
                     /locus_tag="ACFIRM_001823"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC3029392.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            1295..1768
                     /locus_tag="ACFIRM_001824"
     CDS             1295..1768
                     /locus_tag="ACFIRM_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC3029393.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNILELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFDDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            1782..2117
                     /locus_tag="ACFIRM_001825"
     CDS             1782..2117
                     /locus_tag="ACFIRM_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029394.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            2311..5001
                     /gene="glnE"
                     /locus_tag="ACFIRM_001826"
     CDS             2311..5001
                     /gene="glnE"
                     /locus_tag="ACFIRM_001826"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC3029395.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGDKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            5425..7632
                     /gene="uvrD"
                     /locus_tag="ACFIRM_001827"
     CDS             5425..7632
                     /gene="uvrD"
                     /locus_tag="ACFIRM_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC3029396.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         2059 a         2401 c         2047 g         1591 t
ORIGIN      
        1 tttgtcgttc aagtttgacc tgtgtgccgt atgtttggcg ggatttcggt taaaatggcg
       61 gcatttccat ctgaagcaga aagccctgtc atgtatccac ttgcccgccg catcctgttt
      121 gcactcgatg ccgaaaaagc ccaccacttc acgctcgacg cgctcaacac ggtttataaa
      181 ttgggcctga ttcccgtaac cgacaaccgt accaaaccta taaaactgat gggtatggat
      241 ttgcccaatc ctgtcgggct tgccgcaggt ctcgacaaaa acggtgaata catcgacgct
      301 ttgggcgcgc tcggtttcgg cttcctcgaa atcggcacgg ttacgcgcaa cccgcagccc
      361 ggcaacccgc agccgcgcct cttccgcgtt cccgaacacc aaggcatcat caaccgaatg
      421 ggttttaaca accacggcat cgacgcgatg atacgcaata ttgaaaaaag caaatatcaa
      481 ggcgtattgg gcatcaacat cggcaaaaac gcggttacgc ccatccaaaa cgccgccgat
      541 gattatttaa tctgcctcga aaaagcctac gcgcacgcaa gctacattac cgtcaacatt
      601 tcctcgccca acaccaaaaa cctccgcgcg ctgcaaggcg gcggcgaatt gggcgcattg
      661 ctcgaagccc tgaaaaacaa acaggcgcag cttgccgccg cccacggcaa atacatcccg
      721 ctagccgtca aaatcgcccc cgatttggat gaagcacaaa tcgaagacat cgcccgcgtc
      781 gtcaaatccg tcgaaatgga cggcatcatc gctaccaaca ccaccatcga caaatcaagc
      841 ctcggcagcc atccgctcgc aggcgagcag ggcggtttga gcggttttcc cgtgcgcgaa
      901 aaaagcaacc gggtgctgaa aaaactggcg gaacacatag acggcgcatt gccgattatc
      961 ggcgtaggcg gcattatgga aggcggggac gcggcagaga aaatccgctt gggcacgacc
     1021 gccgtccaag tgtacagcgg attgatatac aaaggcccgg cgttggtcaa agaatgtttg
     1081 aaggcattgg cgcgatgacg cgccccaccc aaaatgccgt ctgaacgcat gttttgccgt
     1141 tcagacggca ttttcataga ttggtatccg actgttgaag aggcaatcag ccgtcatttg
     1201 gaagactttt cgtaacaggc gtcaatcgaa aggggcggaa tgacggggca ggcaaaccga
     1261 tgcgtgaaca cggggcaggc aaccggagta aaaaatgaac cttgatttaa ccgctcaaaa
     1321 agtccgcctt tcttggaagg atattctgtg ggggtatgag aataaatact tgggttgggc
     1381 tgatgtggca gcttatgccc gaaaaatgac gctttcagat catgatgaac gcgtgttcaa
     1441 gctatcttta accaataaat ccaatattct tgaattaaag cctgttctag aagatttggc
     1501 ttcggaaacg aggggttatt cccctaaaaa ttggctgtac atcctcctaa atgacgtatt
     1561 ccatagaaag gaagaatttg acgacccttt gggggaagtt gaaaaaattt atgcagattt
     1621 cgattatccg gaagaaatag aatcatttgt caggtatatg ccgcccaaag acggctatat
     1681 cccttctaat catagttatg aagaaaatat tgcccggtta tattctcact gggaacacta
     1741 tttgaacaac ggcggcgggc agggttaaaa ccggcaatcc gatgccgtct gaagcgttat
     1801 ccggcgttca gacggcattt tgttttccga cagtttataa actgtcgttg tttcttgaca
     1861 gaaacaacga ccttatttat ttgaaacgat tggaggacat gattatgggt ttttggggta
     1921 gtgtgggaaa agcagcaaaa gcagtaggcg agggattgat tgaagccggc aatgagcata
     1981 aggcgttgaa aatggaatat gcggagaaat caagtgagga gctgcatgaa atcgtaaaga
     2041 gtgatggttt ttttaaaaat tccacacggg agaaaggtgc ggcttatgct gttttaaaag
     2101 agcgtggcga ggtttgaacg ggaaacggcg gcatttgccg ctgttttttt atcggcaggc
     2161 atccgtccga atatcggggc aaggtttcag acggcatcga aggttgctat gatatagtgg
     2221 cttgacttta aaccggtacg gctccgcctt gccctgattt gaagttcatc cactatcaca
     2281 ttcccgccat cctttcaaac ggaatccgaa atgtccgacc accgcctcga caccgcccgc
     2341 cgccattccc tgttcctcgc ccgccagctc gacaacggca aactcaagcc cgaaattttc
     2401 ctgcccatgc tggacaaggc gttgacggat gaggatttcc aagcctttgc cgactgggac
     2461 aaaatccgcg cggaagaaag cgaggaagaa ttggcgcggc agttgcgcga gttgcgccgt
     2521 tatgtggtgt cgcagattat cgtgcgcgat ataaaccgta tcagcgattt gaacgaagtt
     2581 acccgcacga ttacgctgtt tgccgatttt gccgtcaata ccgcgctgga tttcgcctac
     2641 gcctattatc aggacatgta cggcacgccg atcgggcgtt ataccaaatc gccgcagcat
     2701 ttgagcgtgg tggcgatggg caaggcgggc ggctatgagc tgaacgtgtc ttccgacatc
     2761 gatttgattt tcgtctatcc cgaatcgggc gacaccgacg gcaggcgcga acgggggaat
     2821 caggagtttt ttaccaaagt cgggcagaaa ctgattgcat tgttgaacgg cattactgcc
     2881 gacgggcagg tgttccgcgt cgatatgcgg ctgcggccgg acggtgattc gggcgcgctg
     2941 gtgttgagtg aaaccgcgct ggagcaatat ctgattacgc aggggcgcga atgggaacgc
     3001 tatgcgtggt gcaaaggccg cgtagtcacg ccgtatccga acgacatcaa atcgctggtg
     3061 cgccccttcg tgttccgcaa atatctggat tacggcgcgt atgaggcgat gcgcaacctg
     3121 caccgccaaa tccgcagcga agtcagcaaa aaaggcatgg cggacaacat caaactcggc
     3181 gcgggcggca tccgcgaggt tgaatttatc gcccagattt tccagatgat acgcggcggc
     3241 caaatgcgcg cgctgcaact gaaaggcacg caggaaacgc tgaagaaaat tgccgagatg
     3301 ggcattatgc cgtctgaaaa cgtcgaaacc ctgcttgccg cctaccgctt cctgcgcgat
     3361 gtcgaacacc gcctgcaata ctgggacgac cggcaaaccc aaaccctgcc gatctcgccc
     3421 gaacagcggc aactgctcgc cgaaagcatg ggtttcgaca gttatgccgc tttttcagac
     3481 ggcctcaatg ttcatcggaa caaagtcaat cagttgttca acgaaatttt gagcgaacct
     3541 gaagaacaag cgcaaagcaa tagcgaatgg caatgggcat ggcaggaaaa acccgacgaa
     3601 gaagaacggc taggccgtct gaaagaacac gggttcgatg ccgaagccgt caccgcaagg
     3661 ctcgaacaaa tctgccacgg acataaatac cgccgccttt ccgcacacgc ccagccgcgc
     3721 tttgacacca ttgtgccgct gttcgtacag gcggcggcag agcaaaacaa cccgacagat
     3781 acgctgatgc ggctgttgga ttttctcgaa aacatcagcc tccgctccgc ctatctcgcc
     3841 ttcctcaacg aacatccgca aaccttggca caactggcgc agattatgag ccaaagctcg
     3901 tgggtggcgg cgtatctgaa caaatatccg attttgttgg acgaactcat cagcgcgcag
     3961 cttttggata ccgcgtttga ttggcaggca ctcgccgccg ccctttcaga cggcatcgaa
     4021 gcctgcggcg gcgataccga agcgcaaatg gacacgctgc gccatttcca acacgcccaa
     4081 gtcttccgcc tcgccgtcca agacctcgcc ggattgtgga cggtagaatc cctctctgac
     4141 caactctccg ccctcgccga caccgtcatt gccgccgccc tttcgtgcgc gtgggcggat
     4201 atgcccaaaa aacaccgcga caccccgcaa ttcgccgtca tcggctacgg caagctcggc
     4261 gacaaagaac tcggctacgc ctccgacctc gacttagtct atctttacga cgatccccat
     4321 cccgaagcag gcgacgtgta cagccgcctc gcccgccgtc tgaccaaccg gctttccgcc
     4381 gccaccggcg cgggcagcct ctacgaaacc gacctgcgcc tgcgccctaa tggcgacgcc
     4441 ggtttcctcg cgcacagcat cgccgccttt ggaaaatacc agcgtgaaaa cgcatggaca
     4501 tgggaacacc agtccctcac ccgcgcccgc ttcatctgcg gcacacccga aattcagacg
     4561 gcattcgacc gcatccgcac cgaaatgctg actgccgaac gcgaccaaac cgccttggca
     4621 ggcgaaatca tcgaaatgcg cgaaaaaatg ttccccaccc atccgcctgc cgacagcaac
     4681 gtcaaatacg cgcgcggcgg cgtggtcgac gtcgaattta tcgtccaata tctgatactt
     4741 gcccatgccc gccagtatcc gcaactcttg gacaactacg gcaacatcgc cctattgaac
     4801 atcgccgccg actgcggttt gattgacaaa accctcgccg gacaaagccg caccgcctac
     4861 cgcctctacc gccggcagca gcacaacacc aaattgcgcg acgcggcaaa aaccgaagta
     4921 agcgacgaat tgctgtccca ttacggcaat gtcaggaaat tgtggcggga agtgttcggg
     4981 gaggaagcgg caaccgcttg aacaaaaaat gccgtctgaa gcctgacgat tcgggtttca
     5041 gacggcattt gtttttggct gtgtctgaac aatcggtacg atttcggttg tttgcaaatt
     5101 gaaaggcggt tattcctatg ttcaggcggc aaaccgaaat attttcccca accgataggg
     5161 cgatatcaaa gcctattcga ataccgcctt tacggggaag gggctttccg agcttgacca
     5221 tggtcaggct cggcaaatca gcccgcatcc tgtcgatgcg tccgtccaaa tcggtttgga
     5281 acgccgcaat gtgatatccg cctatcaagg ttttgcgtgc gtgtgtcggc gagggctttg
     5341 ccaaaaccga aatcaaaatc aagttgtcag gcatccttcc gtttctatta aaatagcgca
     5401 ttccattttt cagacggcat ccttatgttt cccgaccaat ccgcccccaa tctgctgcaa
     5461 ggcttgaacc ccgaacaact ctccgccgta acctggccgc cgcaatccgc cctcgttttg
     5521 gcaggcgcag gcagcggcaa aacgcgcgtg ctgaccacgc gtatcgcatg gcttttgcaa
     5581 agcggacaag ccagcgtaca cagcattatg gcggtaacgt ttaccaacaa ggccgccaaa
     5641 gagatgcaaa cccgactcgg cgcaatgatt cccatcaacg tccgcgctat gtggctcggc
     5701 acgttccacg gtttgtgcca ccgctttttg cgcctgcacc accgcgacgc aggcctgccg
     5761 tcttcctttc aaatcctcga cagcggcgac cagctttccc tcatcaaacg cctgctcaaa
     5821 agcctcaaca tcgccgaaga aatcatcgcg ccgcgttcgc tgcaaggctt tatcaacgcg
     5881 caaaaagaat ccggtttgcg cgcttccgtc ttgggcgcgc ccgatccgca cacaagccgc
     5941 atgattgggt gctacgccga atacgacaaa atctgccaac gcgaaggcgt ggtcgatttc
     6001 gccgaactca tgctccgcag ctacgaaatg ctgcaaagta acgaaatcct gcgccggcac
     6061 taccaaaacc gcttcaacca cattctggtc gacgagtttc aagacaccaa caaactgcaa
     6121 tacgcctggc tcaaactcat ggcgggcggt aacgcagcgg tatttgccgt cggcgacgac
     6181 gaccaaagca tttaccgctt ccgcggcgca aacgtcggca acatgaccgc actgatggaa
     6241 gaattccaca tcgacgcgcc cgtcaaactc gaacaaaact accgctccgt cggcaacatc
     6301 ctcgccgccg ccaacgccgt tatcgaaaac aacgacgaac gtctcggcaa aaacctgcgc
     6361 accgacgccg aagcaggcga caaaatccgc tattactccg cctttaccga cctcgaagaa
     6421 gcccggttca tcgtggacga aaccaaagcc ctcgaacgcg aaggctggga tttggacgaa
     6481 atcgccgtcc tctaccgcag caacgcccaa tcgcgcgtca tcgaacaaag cctgttccgc
     6541 agcggcatcc cctacaaaat ctacggcggc ctgcgctttt acgaacgcca agaaatcaaa
     6601 cacgccctcg cctacctgcg cctcgccgtc aatcccgacg acgacaacgc cctcttgcgt
     6661 gtcatcaact tcccaccgcg cggcatcggc gcgcgcaccg tcgaaaacct tcagacggcc
     6721 tcaaacgaac aaggcatcac cctctggcag gcagcctgca acgccggcgc gaaagccgcc
     6781 aaagtcgccg ccttcgtccg cctgattgaa gccctgcgca accaagtcgg acaaatgcac
     6841 ctgtccgaaa tcatcgtcgg catcctcaaa gacagcggtc tgaccgagca ctaccgaacc
     6901 caaaaaggcg acaaccaaga ccgcctcgac aaccttgacg aactcgtcaa cgccgccatc
     6961 gagttcaaac ccgaagacag caactttgaa accctgcccg aaaacatttc agacgatccc
     7021 gccttcccca tcctcgcctt cttgagcaac gccgccctcg aatccggcga aaaccaagca
     7081 ggggcaggcg aaaaagccgt ccaactgatg accgtccacg ccgccaaagg cttggaattc
     7141 aacgccgtct tcctcaccgg tatggaagag gggcgtttcc ccagcgaaat gagccttgcc
     7201 gagcgcggcg gcctcgaaga agaacgccgc ctcatgtacg tcgccatcac ccgcgcccgc
     7261 aaacgcctct acatcaccat ggcacagcaa cgcatgctgc acggacaaac ccaattcggc
     7321 atcgcatccc gcttcgtcga agaaatcccg ccagaagtat tgcactacct gtccgtcaaa
     7381 aaacccgcct tcgacagcta cggcaacacg cgccaaacca ccgtacaacg ggacaaaatc
     7441 atcgatgact tcaaacagcc gcaaacctac gcaggcttcc gcatcggaca aaacgtccgt
     7501 cacgccaaat tcggcaccgg cgtgattatc gatgccgcag acaaaggcga atccgcccga
     7561 ctgaccatca acttcggcaa acagggcgtg aaagagctgg ataccaagtt tgcgaaattg
     7621 gaagcgatgt aaatttgaaa tgcaggccgg atattcgtat ccgacaaaaa aacatttgac
     7681 gcgtttatcg tttccgaaaa accgctgttg gaaatgtcgg attcgagaat ccgacctacg
     7741 gcaaaaaacc gtagcaagga caaggcaaat agtcgtctga aaacgggaag ggcaattttg
     7801 ccgcagccgc cgccgtcatt cccgcgaacc tacattccgt cattcccacg aaagtgggaa
     7861 tccggaacgc aaaatctaaa gaaaccgttt tatccgataa gtttccgcac cgacaggtct
     7921 ggattcccgc ttatgcggga atgacggcgg cgggaatcgg cggattcggc ggagttggtg
     7981 gatttggcgg gctgaagccc gcccttgtat atcggaactt ccgtatcata gcgacaaacc
     8041 gcccggccgc cacccgcgcc cgcccaaggc agacaaccgt tgcgcggctc agggagcg
//
LOCUS       DAXFHF010000078         7751 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXFHF010000078 DAXFHF010000000
VERSION     DAXFHF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.078"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFIRM_001828"
     CDS             complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFIRM_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC3029397.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFIRM_001829"
     CDS             complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFIRM_001829"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC3029398.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLEKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1416..1823)
                     /locus_tag="ACFIRM_001830"
     CDS             complement(1416..1823)
                     /locus_tag="ACFIRM_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC3029399.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1823..2509)
                     /locus_tag="ACFIRM_001831"
     CDS             complement(1823..2509)
                     /locus_tag="ACFIRM_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC3029400.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQAMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2629..3672
                     /gene="pilT"
                     /locus_tag="ACFIRM_001832"
     CDS             2629..3672
                     /gene="pilT"
                     /locus_tag="ACFIRM_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC3029401.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="ACFIRM_001833"
     CDS             3836..5062
                     /locus_tag="ACFIRM_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC3029402.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5179..7329
                     /gene="yccS"
                     /locus_tag="ACFIRM_001834"
     CDS             5179..7329
                     /gene="yccS"
                     /locus_tag="ACFIRM_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC3029403.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCGSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1890 a         2585 c         1701 g         1575 t
ORIGIN      
        1 gtttgcctcc atcagtcggc aaactgtttc ttcatcctct ctcggcgttc ttgggcctca
       61 acggataaag tggctgtcgg gcgtgccagc agccgcttca gaccgatagg ttctcccgta
      121 tcggcacaga atccatagtc cccttcatca atattgcgga tggtcgcctg tattttactg
      181 agaagttttc gttcccgatc ccgggtacgg agttccaatg cgtactcttc ttcctgtgtg
      241 gcacggtcgg caggatcggg ggctgattcg tgttcttgga gatgccctgt tgtaacggag
      301 gcgttttcga tgagttcgtc ttgcattttt accagcaatt cgcggaaaaa agccaaatgg
      361 tcgtcattca tataatcgtc ttccggccca atccaattca aaatatcttg ttctgtcagc
      421 tttaccataa tgtatgccct ctcggttcac cgcaaaaaaa caaagcacta caaagtggct
      481 ttattccttt gatttttata aggcttattg cctaaggcgt ttttgaaaac gcgcatcata
      541 acacgttttg gattgttttt tttattttct ttacattatt gatattgccg ttccatttcc
      601 tgcgaacggc acacacaggc acaaacgccc tcacttatgg cttcggcgac acgatgccgt
      661 ctgaaggctt ccacggcttc gtgggttgtc ccgccttttg acgttacatt tttttgaagc
      721 ttctcgaaat cttcacccgt ctgctcggca agggcaaccg ctcctttaaa cgttgccaga
      781 ctgagcgcgc gtgcttctgc catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg
      841 tccagcagat aaaacacata agccggcccg ctgccgctga tgccggtaat gctgtgcatt
      901 tgcgcttcat cttccaacca aacggtcaaa ccgactgatt tcatgattcg atcggcaatc
      961 ctgcggtctg tttccgatac ttccgcttcg gcatacatac cggatacgcc cagcccgatt
     1021 tttccaggtg tattaggcat aacccggaca atgcggcggc ttcccccgag gtaacggctg
     1081 agcgtaccga ccgacaatcc ggctgcgaca gaaagcacca atgcgccgtt ggtgcggata
     1141 tttttgcacg cggcttccat atcctgcggt ttgacggcaa ggattaaaac atcgtcggaa
     1201 tgaagctccg gcagggttgc cgaagtttcg acccccaact ctttttccaa acgttcgcgc
     1261 ttttccgcac cacggttggc tatatggatg cggtatcccc cttgtttgac caatccgccc
     1321 gcaacggcag ccgccatatt tccgccgccg agaaaataaa cattcattat ttctacccta
     1381 ttcaaaatta ccttactaaa ttcattccca attttttacc atcggacgcg ctcggacagg
     1441 ccgttgcgcc gcaacccctg cttttccaaa cagtcgaaac cttcctgttt gaacctctca
     1501 accaaaggtt tgccgtcacg cgtacgccaa tccaacccgc aggcttcaaa cttccctatt
     1561 ttcctataaa agtaagattt ttgcgcggat tcatattcgt ttgccctgac ggaacgattg
     1621 ccgtctgaaa gcggtatctc gtagtcctct aaagacagcg gactctcgcc gccaatatcc
     1681 caaaaatcga tggcggcagg ttttctcggc ttacaccagc cggacaagga cgaacactca
     1741 taccaccccg ccagccggca gcctgcaacc gtaaatgcaa acaacgcagc cgttgccgta
     1801 tattttatta cccgaatttc catcagcccc ttttcccgaa aatcgcgctg ccgatacgga
     1861 cgtgtgtcgc accgcactca atggcggcag gcatatcgtc cgacatcccc atagacagca
     1921 cgtccgcctt aacgccagcc gcattgaggt cggcaagcag cttttgcatg gcctgaaact
     1981 gagttttcaa ctccgtttca ctgctgttgg ctttggcaac gcacatcagt ccgcgcacga
     2041 caaggttggg cagcttcgcc acctccacgg caagcgcgac cgcttcttcg ggcgcgacac
     2101 cgtgttttgc cgcctcgccc gcaatgttca cctcgacaca cacctgcaag ggcggcatta
     2161 aggagcgacg ctgcctgctc agccggacgg cggtcttcag acggcatacg gtatgcaccc
     2221 aatgcgcgcg ttcggcgaca aatttggttt tgttggactg cacatcgccg atgacgtgcc
     2281 acacgatgtc ggtcaaatcc gccaattcct ccgttttgcc gtaccactcc tgaatatagt
     2341 tctcgccgaa atcacgctgt ccggcagcgt aaacttcgcg gatgccgtct gaagggaagg
     2401 tcttgctgac ggcaatcagg ctgacggaat gcggctccct gcccgcctgc agaaccaatt
     2461 ttccgatacg gtcggacacc tcacgataac gttcttgcaa caccgtcata gatcatcccc
     2521 taattaaaaa tgattaaaca gttgaaaccc ctccagtcag ggcggtataa tcaaggttgt
     2581 tagaatcatt ccaaccaatc gaaacattat actaaacaga gccgcattat gcagattacc
     2641 gacttactcg ccttcggcgc taaaaacaaa gcatccgacc ttcacctgag ttcgggcata
     2701 tcccctatga ttcgggttca cggcgatatg cggcgcatca accttcccga aatgagcgcg
     2761 gaagaggtcg gcaatatggt aacttcggtg atgaacgacc accagcggaa aatctaccag
     2821 caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac
     2881 gccttcaaca ccggccgcgg ccccgccgcc gtattccgca ccattcccag caccgtctta
     2941 tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc
     3001 atggtattgg ttaccggccc taccggttcg ggcaaatcga ccacgcttgc cgcgatgatc
     3061 aactacatca acgaaaccca gccggcacac atcctgacca tcgaagaccc gatcgaattc
     3121 gtccaccaaa gcaaaaaatc cctgattaac caacgcgagc tgcaccagca caccctcagc
     3181 ttcgccaacg cgctgagttc cgcattgcgc gaagaccccg acgttatcct tgtcggcgag
     3241 atgcgcgacc ccgaaaccat cggcttggca ctgaccgccg ccgaaaccgg acacttggtt
     3301 ttcggcacgc tgcacacgac cggcgcggca aaaaccgtcg accgtatcgt ggacgtattc
     3361 cccgccggag agaaagaaat ggtgcgttcc atgctgtccg aatcgctgac cgccgtcatc
     3421 tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg
     3481 attgccaacc ccgccgtccg caacctcatc cgcgaaaaca aaatcacgca gattaactcc
     3541 gtcctgcaaa ccgggcaggc gagcggtatg cagacgatgg accaatcgct gcaatcgctg
     3601 gtgcgccaag ggctgatcgc accggaagcc gcacgcagac gcgcgcaaaa cagcgaaagt
     3661 atgagtttct gacacacaac cgccttccgg ccataccggc gggaaaacaa ggcgcaaaca
     3721 cgccgggcgg gacgcggcat cccgcccggc atacccttcc gaacaggaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aacggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cacacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggctctga cgggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccaatcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ctacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcgggaattg ggcttacccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgcccg gccatatcgt taccatcgaa gacccgattg agtttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc cccagacgtg gtctgtatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aaccaaatac tgattgacat cgccctcaat ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaaaaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaggact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggaccaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aacgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgcc acactttatt cacttcataa
     5161 caataaaccg gtaaaaccat gaaaacccca ctcctcaagc ctctgctcat tacctcgctt
     5221 cccgttttcg ccagtgtctt taccgccgcc tccatcgtct ggcagctagg cgaacccaag
     5281 ctcgccatgc ccttcgtact cggcatcatc gccggcggcc tggtcgattt ggacaaccgc
     5341 ctgaccggac ggctgaaaaa catcatcgcc accgtcgccc tgtttaccct ctcctcgctc
     5401 acggcgcaaa gcaccctcgg cacagggctg cccttcatcc tcgccatgac cctgatgacc
     5461 ttcggcttta ccattttagg cgcggtcggg ctgaaatacc gcaccttcgc cttcggcgca
     5521 ctcgccgtcg ccacctacac cacgcttacc tacacccccg aaacctactg gctgaccaac
     5581 cccttcatga ttttatgcgg caccgtactg tacagcaccg ccatcatcct gttccaaatc
     5641 atcctgcccc accgccccgt ccaagaaagc gtcgccaacg cctacgaagc actcggcggc
     5701 tacctcgaag ccaaagccga cttcttcgac cccgatgagg cagcctggat aggcaaccgc
     5761 cacatcgacc tcgccatgag caacaccggc gtcatcaccg ccttcaacca atgccgttcc
     5821 gccctgtttt accgtttgcg cggcaaacac cgccacccgc gcaccgccaa aatgctgcgc
     5881 tactacttcg ccgcccaaga catccacgaa cgcatcagct ccgcccacgt cgactaccaa
     5941 gagatgtccg aaaaattcaa aaacaccgac atcatcttcc gcatccaccg cctgctcgaa
     6001 atgcagggac aagcctgccg caacaccgca caagccctgc gcgcaagcaa agactacgtt
     6061 tacagcaaac gcctcggacg cgccatcgaa ggctgccgcc aatcgctgcg cctcctttca
     6121 gacggcaacg acagtcccga catccgccac ctgagccgcc ttctcgacaa cctcggcagc
     6181 gtcgaccagc agttccgcca actccgacac agcgactccc ccgccgaaaa cgaccgcatg
     6241 ggcgacaccc gcatcgccgc cctcgaaacc ggcagcttca aaaacacctg gcaggcaatc
     6301 cgtccgcagc tgaacctcga atcaggcgta ttccgccatg ccgtccgcct gtccctcgtc
     6361 gttgccgccg cctgcaccat cgtcgaagcc ctcaacctca acctcggcta ctggatactg
     6421 ctgaccgccc ttttcgtctg ccaacccaac tacaccgcca ccaaaagccg cgtgtaccaa
     6481 cgcatcgccg gcaccgtact cggcgtaatc gtcggctcgc tcgtccccta cttcaccccc
     6541 tccgtcgaaa ccaaactctg gatcgtcatc gccggtacca ccctgttctt catgacccgc
     6601 acctacaaat acagtttctc caccttcttc atcaccattc aggcactgac cagcctctcc
     6661 ctcgcaggtt tggacgtata cgccgccatg cccgtgcgca tcatcgacac cattatcggc
     6721 gcatcccttg cctgggcggc ggtcagctac ctgtggccag actggaaata cctcacgctc
     6781 gaacgcaccg ccgcccttgc cgtatgcggc agcggcacat acctccaaaa aattgccgaa
     6841 cgcctcaaaa ccggcgaaac cggcgacgac atagaatacc gcatcacccg ccgccgcgcc
     6901 cacgaacaca ccgccgccct cagcagcacc ctttccgaca tgagcagcga acccgcaaaa
     6961 ttcgccgaca gcctgcaacc cggctttacc ctgctcaaaa ccggctacgc cctgaccggc
     7021 tacatctccg ccctcggcgc ataccgcagc gaaatgcacg aagaatgcag ccccgacttt
     7081 accgcacagt tccaccttgc cgccgaacac accgcccaca tcttccaaca cctgcccgac
     7141 atgggacccg acgactttca gacggcattg gatacactgc gcggcgaact cggcaccctc
     7201 cgcacccgca gcagcggaac acaaagccac atcctcctcc aacagctcca actcatcgcc
     7261 cggcagctcg aaccctacta ccgcgcctac cgacaaattc cgcacaggca gccccaaaac
     7321 gcagcctgaa aaaatttcgg cattttgtaa agggggggcg gattgtcagg caggttgcaa
     7381 gatagtggat aaggctttgc gcagggtaaa tgcgtagcaa ctgaaccgtc attccctcaa
     7441 aaacagaaaa ccaaaaacag aaacctaaaa tcccgtcatt cccacgaaag tgggaatccg
     7501 gttttttgag tttcagtcat tcccgataaa ttgccttagc attgaatgtc tagattcccg
     7561 cctgcgcggg aatgacggta gagcggtttc tgttttttcc gataaattcc taaaactcaa
     7621 aatttcatca ttcctacaaa aacagaaaat caaaaacaga aacctaaaat cccgtcattc
     7681 ccacgaaagt gggaatccag aatctctaaa gcttcagcta acctttgaat attgctgttg
     7741 ttctaaggtc t
//
LOCUS       DAXFHF010000079         7342 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXFHF010000079 DAXFHF010000000
VERSION     DAXFHF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.079"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            701..1105
                     /locus_tag="ACFIRM_001835"
     CDS             701..1105
                     /locus_tag="ACFIRM_001835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC3029404.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            1145..2326
                     /locus_tag="ACFIRM_001836"
     CDS             1145..2326
                     /locus_tag="ACFIRM_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC3029405.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVTLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(2429..4684)
                     /locus_tag="ACFIRM_001837"
     CDS             complement(2429..4684)
                     /locus_tag="ACFIRM_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC3029406.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            5057..6235
                     /gene="nirK"
                     /locus_tag="ACFIRM_001838"
     CDS             5057..6235
                     /gene="nirK"
                     /locus_tag="ACFIRM_001838"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC3029407.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            6413..7168
                     /locus_tag="ACFIRM_001839"
     CDS             6413..7168
                     /locus_tag="ACFIRM_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC3029408.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         1848 a         2056 c         1836 g         1602 t
ORIGIN      
        1 cctttgtcat cccttgctta tctcccatgt gcgaatcctc aaaaacgggc aaaaaaaaaa
       61 gccctgttac ttgtagaaag taaagggcgt taatttttgt taatcgcccc ttcttaggga
      121 cgcaatatat aaggtttcag acggcattgt tctcccgccg tcattcccgc gcaggcggga
      181 atccggaacg taaaatctaa agaaaccgtt ttatccgata agtttccgtg ccgacaggcc
      241 cggattcccg cctgcgcggg aatgacggga ttttaggttt ctgattttgg ttttctgttt
      301 ttgaggaaat gacgggattt tagtaaccgt aacaactgcc tgcgcgacgg ctaaggggct
      361 tcagcaaccg tagcaactgc ctgtgtggga atgacggaca atgggcttca gacggcatct
      421 cttgccttcc gctaaaacag tttgccgcac aactgttcaa acgcgtccga tatgtttcaa
      481 cacacaggac gacacataaa gcaccgccct atatgtcgtc ctgattcgga aggggttacg
      541 cccctcccaa ataaagtctg atcctgccgc cctaaagggc ggggtttcaa ccgaaaagga
      601 aacacgatga agtggtacaa ttaacggcaa tgcggacaga caaattaaac tatcagaaat
      661 tgccctaccg ttttttaaac actttcagga ataaggaaaa atgaccgccc aaccctgccc
      721 catctgcacg gcacaagatg aagatatttt gctgcaaacc cccaagctgc gcgtcatcgc
      781 cgtccataac gacagcggtt cgccggcatt ctgccgcgtc atttggcacg gacacattgc
      841 cgaaattacc gacctttcgg cagcggaacg cggcgaattg atggaaatgg tgtacaaagt
      901 cgaagccgct atgcgccaag tgttccggcc ggcaaaaatc aacctcgcca gcttgggcaa
      961 tgtcgtgccg cacctgcatt ggcatattat cgcccgcttt gaaaacgatg cgactttccc
     1021 tgcgccgatt tgggcaaacc ccgttcgaaa acacggtatg accctgccgc aaaattggac
     1081 ggaacagctt aaaaagctgc tttaaacccg ccgatgccgt ctgaaaccgt atgaaaggga
     1141 aattatgacc gaaccgacct cccgccgccg ttttctgaaa acctgcaccg ccgccggcgc
     1201 ggggctgctt caggcttgcg gcacatccgc cacatccgtt ccgccccttc cctcttccca
     1261 ttccgttgtg aaagcccgaa ccgtgactct ccaaacgcca cgccgtcaaa gttcggacgg
     1321 caaccttctg cgcgttgtcg cttcgtcagg atttgccgaa gacaccaacc gcgtcaacac
     1381 agccttaacc cgcctttaca atgccggttt taccgtaacc aaccaacagg cgggcagccg
     1441 ccgtttccaa cggtttgccg gcacggacgc gcaacgcgcg gcagatttcc aagaggtcgc
     1501 ctccggccgc gtcgccacgc ctaaagtgct gatgggcttg cgcggcggtt acggtgcggc
     1561 gcggattctg ccgcatatcg attttgcctc gctcggcgca aggatgcgcg aacacggcac
     1621 gctgtttttc ggattcagcg acgtatgcgc cgtccaactg gcattgttgg caaaaggcaa
     1681 tatgatgagt tttgccggcc cgatggctta tagcgatttt ggcaaacctg cccccggtgc
     1741 gtttacgatg gatgccttta tcaagggggc aacccaaaac cgcctgaccg ttgatgttcc
     1801 ttatatccaa cgcgccgatg tcgaaaccga aggcacgttg tggggcggca acttaagcgt
     1861 cctcgcctcg ctcgccggta cgccttatat gcccgacatc gacggcggca ttttgttcct
     1921 cgaagatgtc ggcgaacagc cctaccgcat cgaacgtatg ctcaatacgc tgtatctttc
     1981 gggtattttg ggcaaacagc gcgccatcgt gttcggcgat ttccgtatgg aaaaaattcg
     2041 agatctctat gattcgtctt atgatttttc cgccgttgcc aagcatattt cgcgcacggc
     2101 gaaaatcccc gtgctgacgg gcttcccgtt cggacacatt gccgacaaaa tcactttccc
     2161 tctgggcgcg cacacccgaa tccgtatgaa cggaaacggc ggctattcgg tcgcgtttga
     2221 aggctacccc acactcgatg cgtccgccct gactttggat accctgctcc caccgccgga
     2281 tttgcctatc ttccccgaaa gcggtgttgc cgatatttcg gaataaaccc gcaaacggac
     2341 aaatgccgtc tgaagccttc agacggcatt ttcctgtaaa aaaaaccgca cctgatggca
     2401 ggtgcggttt ccattttcag gctaaaactt atttgtcgcg gccgaatacg attttagtgg
     2461 cttggatggc aacgcagatt gcaccgccga taaagattaa gtcgggggca gtgcgtaccc
     2521 agcgcagggt gtcgaggatt tccatttgca ggaattcttc gctgcgggca taccacaaac
     2581 cgtgcgtgat agaggcgtat gcctgaatca cgccgacagg cagcaggctg atggcaatca
     2641 taccgaccaa gccgccgttg agcagccaga agccccaagt catcagtttg tcgtcaaacc
     2701 gcgcgttcgg tttcaagtag cgcgcaacca acaatacgaa gcccaatgcc aagaaaccgt
     2761 acacaccgaa caaggcggcg tgcgcgtgaa cggcggaagt gttcaaacct tggatataga
     2821 acagggaaat cggcggattg atcagaaagc cgaatacgcc ggcaccgatc atattccaaa
     2881 aagcgactgc cacgaagcac atcagcggcc aacgcaggcg tttcgcccag tcggacaggt
     2941 gttggtaaga ccagtgctcg tatgcctcgc ggcccagcaa caccagcggc acgacttcca
     3001 aagcggagaa gcaggcgccg attgccatag aggcggaggt agagccggag aagtacaggt
     3061 ggtgcagcgt gcccggaacg ccgcccaaca taaagatggc ggcagcggcc aaagtggagg
     3121 cagtggcggt actgcggcgg acaaagccca tattgtagaa gacaaaggca aaggcggcag
     3181 tggcaaatac ttcaaagaag ccttccaccc acaggtggac cacccaccaa cgccagtatt
     3241 ccattacggc aatcggggat ttttcgccat agaacaggcc cggtgcgtag aacacgccca
     3301 cgccgaccat agaagcgaca aagattgcca gcaagttttt gtccacgcct ttttctttga
     3361 aggcggaaac cgtgcagcgc aacatcagga acagccacaa cagcagtccg accatcaaaa
     3421 ggagttgcca gaaacgtccc aaatcgaggt attcgtaacc ttggtgtccg aaccagaagt
     3481 taaattcggg aggaaggatg tgcgtcaacg cgaagaagtt gcccgcgtaa gaaccgccga
     3541 ccacgatgaa gagggcgata tagaggaagt tcacgcctgc acgttggaac ttgggatctt
     3601 tgccgccgtt gacaatcggc gcgaggaaca aacctgccgt caaaaagccg gttgcaatcc
     3661 agaagatggc ggattggatg tgccaagtac gggtcagggc gtaggggaac cagtcggaca
     3721 tttcaaagcc caacgcctcg tcaatgccgt agaaaccctg accttcgacg gtgtagtgcg
     3781 cggtcaggcc gcccagcaat acttgtacca caaacagggc gaccgtcagg aagacgtatt
     3841 tgcccaatgc tttttgcgaa ggggtcagtt ggattttgga aatcgggtct tcagacggca
     3901 cttccacttc ctcgtgtttg gtcaggaagg aataacccca catcaacaaa ccgatgccca
     3961 tcaagagcag tacgacactg gtaaacgacc acatgtagtt ttcagtagtc ggtacattgt
     4021 tgatcaaagg ctcgtgcggc cagttgttgg tgtaggtaaa gacctcgccg ggacggttgg
     4081 tcgaagcaga ccaagaagtc cagaagaaga agtcgaacag tttttcacgc gcttcttggc
     4141 ttggcaatgt gttgtttttc attgcaaagt gttcgcgggt ggtttgcaac ttgggatcat
     4201 cgccgtaaac gccgtgatag taaggcagga tgctttcgat ggctttcacg cgcgtatcgc
     4261 tgatgacgac gctgccgtct tctttaatac ggctttgatt gcggtattca tcggccaagc
     4321 gggttttcag aacggcttgt tcttcagggg aaacttcatc gaattttttg ccgtaagcct
     4381 gttgcgcggt caaatccaac caggcggaca actcacgatg cagccagtcg gccgtccagt
     4441 ccggagcctg atatgcgccg tgacccagaa tcgaaccgac ttccatgccg ccggtactct
     4501 gccacgcaga ctgacctgcc aaaatatcgt ctttcgtcat cagcactttg ccggatgcgg
     4561 aaacgacctg ttcggggtaa ggcggggctt tcttataaac ctcgctgccc atatagccaa
     4621 gaatggtaaa gcataccgcc agaacggcaa acagcaagta ccacagcttc ttgtactgtc
     4681 ccattttgag agctcctttt aataaatcag ttgtttaaaa ttcacaaaat atgaatgtta
     4741 aagattgtag catggtttac cgcgcaaata aacatttgtt caaacaaact cacatataga
     4801 acaaatacat atatgataat aactatcatt attctttact cggaaattgc cctgcctttg
     4861 cctgctctgc cggagcccct agcaaatcag cctattcatt gtaattttta gtagttataa
     4921 agtattagaa gcgtcatttt aagttcatat tttatgaatt atttgactta aattaaaatg
     4981 cccgcaatgg gacaaccgca taatcacacc aagttcttaa ctaatccccc tacttttctt
     5041 acaaaaggaa aacattatga aacgccaagc attagccgca atgattgctt ccttattcgc
     5101 attggccgcc tgcggtggcg aacaagctgc tcaagcccct gccgaaaccc ctgccgcttc
     5161 cgcagaagcc gcaagttccg ccgcacaagc taccgccgaa acgcctgcag gcgaactgcc
     5221 cgtcatcgat gcggtgacca cccacgctcc cgaagtacct cccgcaatcg accgcgacta
     5281 tcctgccaaa gtacgcgtaa aaatggaaac cgtcgaaaaa accatgaaaa tggacgacgg
     5341 ggtggaatac cgctactgga catttgacgg cgacgttccg ggccgtatga tccgcgtacg
     5401 cgaaggcgat acggttgaag tcgaattctc caacaatcct tcttctaccg ttccgcacaa
     5461 cgtcgacttc cacgccgcaa ccggtcaggg cggcggtgca gccgcgacct ttaccgcccc
     5521 gggccgcact tccacattca gcttcaaagc cctgcaaccg ggcctgtaca tctaccactg
     5581 cgccgtcgcg ccggtcggta tgcacatcgc caacggtatg tacggtctga ttttggtcga
     5641 gcctaaagaa ggcctgccga aagtggataa agagttctac atcgtccaag gcgacttcta
     5701 caccaaaggc aaaaaaggcg cgcaaggcct gcaaccgttc gatatggaca aagccgttgc
     5761 cgaacagcct gaatacgtcg tattcaacgg tcatgtaggt tctatcgccg gcgataacgc
     5821 cctgaaagcc aaagcaggcg aaaccgtgcg tatgtacgtc ggtaacggcg gcccgaactt
     5881 ggtgtcttcc ttccacgtca tcggcgaaat cttcgacaaa gtttatgttg aaggcggcaa
     5941 actgattaac gaaaacgtac aaagcaccat cgtgcctgcc ggcggttctg ccatcgtcga
     6001 attcaaagtc gacatcccgg gcagctacac tttggtcgac cactccatct tccgcgcatt
     6061 caacaaaggc gcgttggggc aattgaaagt agagggtgcg gaaaaccctg aaatcatgac
     6121 tcaaaaattg agtgataccg cttacgccgg cagcggcgcg gcttctgccc ctgctgcttc
     6181 cgcaccggct gcttctgccc cggcagcctc tgcatccgaa aaaagcgttt attaaatcgg
     6241 atacccgtca ttagcgggac aaaccactgc cgccgtactt cattacgcac ggcagtggtt
     6301 ttttaacaac caatctttcc tttcggaaga ttgatttttc agacggcatt cgcggctttt
     6361 tgtttaaaat gccgtctgaa aaatcaatgc taaccgcctg tcaggaggct ttatgaagta
     6421 cgtccggtta tttttcctcg gcacggcact cgccggtact caagcggcgg ctgccgaaat
     6481 ggttcaaatc gaaggcggca gctaccgccc gctttatctg aaaaaagata ccggcctgat
     6541 taaagtcaag ccgttcaaac tggataaata tcccgttacc aatgccgagt ttgccgaatt
     6601 tgtcaacagc cacccccaat ggcaaaaagg caggatcggt tccaaacagg cagaacccgc
     6661 ttacctgaag cattggatga aaaacggcag ccgcagctat gcgccgaagg cgggcgaatt
     6721 aaaacaaccg gtaaccaatg tttcctggtt tgccgccaac gcctattgcg ccgcacaagg
     6781 caaacgcctg ccgaccatcg acgaatggga atttgccgga cttgcttccg ccacgcagaa
     6841 aaacggctca aacgaacccg gctacaaccg cactattctc gattggtatg ccgacggcgg
     6901 acggaaaggc ctgcacgatg tcggcaaagg ccgcccgaac tactggggtg tttatgatat
     6961 gcacgggctg atttgggaat ggacggaaga tttcaacagc agcctgcttt cttccggcaa
     7021 tgccaacgcg caaatgtttt gcagcggcgc atctgtcggg gcgagcgact cgtccaacta
     7081 tgccgccttc ctccgctacg gcatccgcac cagcctgcaa tccaaatacg tcctgcacaa
     7141 cttgggcttc cgctgcgcaa gccgataacc ccttcaatta tagtggatta acaaagacca
     7201 gtacggcgtt gcctcgcctt gccgtactgg tttttgttaa tccgctatat tccgccatct
     7261 ctaagattta cagcgataca cgggtaattt aaggaatgcc cgaaccgtca ttcccgcaac
     7321 ttttcgtcat tcccgcgaaa gc
//
LOCUS       DAXFHF010000080         7177 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXFHF010000080 DAXFHF010000000
VERSION     DAXFHF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.080"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            89..2251
                     /gene="fetA"
                     /locus_tag="ACFIRM_001840"
                     /gene_synonym="frpB"
     CDS             89..2251
                     /gene="fetA"
                     /locus_tag="ACFIRM_001840"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC3029409.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGGENSRINITR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRIATRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFSIPIKEKKNGQEVDK
                     PMEQQKKDRADEAIVRSYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHD
                     GKTVSSSSLNPSFGVIWQPREHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFKN
                     PNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2403..3368
                     /locus_tag="ACFIRM_001841"
     CDS             2403..3368
                     /locus_tag="ACFIRM_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3029410.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDSGPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3386..3577
                     /locus_tag="ACFIRM_001842"
     CDS             3386..3577
                     /locus_tag="ACFIRM_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029411.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3596..4564
                     /locus_tag="ACFIRM_001843"
     CDS             3596..4564
                     /locus_tag="ACFIRM_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3029412.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLTGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4554..5528
                     /locus_tag="ACFIRM_001844"
     CDS             4554..5528
                     /locus_tag="ACFIRM_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC3029413.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFDFELVIMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALILLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5573..6199
                     /locus_tag="ACFIRM_001845"
     CDS             5573..6199
                     /locus_tag="ACFIRM_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC3029414.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6283..7041
                     /locus_tag="ACFIRM_001846"
     CDS             6283..7041
                     /locus_tag="ACFIRM_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3029415.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(7076..>7177)
                     /locus_tag="ACFIRM_001847"
     CDS             complement(7076..>7177)
                     /locus_tag="ACFIRM_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3029416.1"
                     /translation="RKPFPPFLRECEFRLNFGTPPRQLKILRDRCGI"
BASE COUNT         1650 a         2159 c         1869 g         1499 t
ORIGIN      
        1 caaaaataat tattattatt ttttcttatc ctgccaaacc ttaacggttt ggcttaactt
       61 cccttcatac actcaaaagg acgaacaaat gaacgccccg tttttccgcc tcagcctgct
      121 ctcgctcaca cttgccgccg gctttgccca cgcggcagaa aataatgcca atgtcgcatt
      181 ggataccgtt accgtaaaag gcgaccgcca aggcagcaaa atccgtacca acatcgttac
      241 gcttcaacaa aaagacgaaa gcaccgcaac cgatatgcgc gaactcttaa aagaagagcc
      301 ctccatcgat ttcggcggcg gcaacggcac gtcccaattc ctgacgctgc gcggtatggg
      361 tcagaactct gtcgacatca aggtggacaa cgcctattcc gacagccaaa tcctttacca
      421 ccaaggcaga tttattgtcg atcccgcttt ggttaaagtc gtttccgtac agaaaggcgc
      481 gggttccgcc tctgccggta tcggcgcgac caacggcgcg atcatcgcca aaaccgtcga
      541 tgcccaagac ctgctcaaag gcttggataa aaactggggc gtgcgcctca acagcggctt
      601 tgccggcaac aacggcgtaa gctacggcgc aagcgtattc ggaaaagagg gcaacttcga
      661 cggtttgttc tcttacaacc gcaacgatga aaaagattac gaagccggca aaggcttccg
      721 caatgtcaac ggcggcaaaa ccgtaccgta cagcgcgctg gacaaacgca gctacctcgc
      781 caaaatcgga acaaccttcg gcgacggcga ccaccgcatc gtgttgagcc atatgaaaga
      841 ccaacaccgg ggcatccgca ctgtgcgtga agagtttgcc gtcggcggcg aaaattcacg
      901 gataaatatt acacgccaag ccccatccta ccgcgaaacc acacaatcca acaccaattt
      961 ggcgtacacc ggcaaagatt tgggctttgt cgaaaaactg gatgccaacg cctatgtgtt
     1021 ggaaaagaaa cgctattccg ccgatgacaa agataacggc tacgcaggca atgtaaaagg
     1081 ccccaaccat acccgaatcg ccactcgggg catgaacttc aacttcgaca gccgccttgc
     1141 cgaacaaacc ctgttgaaat acggcatcaa ctaccgccat caggaaatca aaccgcaagc
     1201 gtttttgaat tcggaattct ccatcccgat aaaagaaaag aaaaacggtc aagaagtcga
     1261 taaaccgatg gaacaacaaa agaaagaccg tgcagatgaa gccattgtcc gttcctaccg
     1321 cctgaccaac ccgaccaaaa ccgataccgg cgcgtatatc gaagccattc acgaaattga
     1381 cggctttacc ctgaccggcg ggctgcgtta cgaccgcttc aaggtgaaaa cccacgacgg
     1441 caaaaccgtt tcaagcagca gcctcaaccc gagtttcggc gtgatttggc agccgcgcga
     1501 acactggagc ttcagcgcga gccacaacta cgccagccgc agcccgcgcc tgtatgacgc
     1561 gctgcaaacc cacggcaagc gcggcatcat ctcgattgcc gacggcacca aagccgaacg
     1621 cgcgcgcaat accgaaatcg gcttcaacta caacgacggc acgtttgccg caaacggcag
     1681 ctacttccgg cagaccatca aagacgcgct tgccaatccg caaaaccgcc acgactccgt
     1741 cgccgtccgc gaagccgtca acgccggcta catcaaaaac cacggttacg aattgggcgc
     1801 gtcctaccgc accggcggcc tgaccgccaa agtcggcgta agccgcagca aaccgcgctt
     1861 ttacgatacc catcctaaaa aactgttgag cgcgaacccc gagtttggcg cacaaaccgg
     1921 ccgcacttgg acggcctccc ttgcctaccg cttcaaaaac ccgaatctgg aaatcggctg
     1981 gcgcggacgc tatgttcaaa aagctacggg ttcgatattg gcggcaggtc aaaaagaccg
     2041 cgacggcaaa ttggaaaacg ttgtacgcca aggtttcggt gtgaacgacg tcttcgccaa
     2101 ctggaaacca ttgggcaaag acacgctcaa tgttaatctt tcggttaaca acgtgttcga
     2161 caagttctac tatccgcaca gccaacgctg gaccaatacc ctgccgggcg tgggacgtga
     2221 tgtacgcttg ggcgtgaact acaagttcta aaacgcacat cccgaaaaaa tgccgtctga
     2281 aagcctttca gacggcatct gtcctgataa tttgatatag attatcattt atcctttcta
     2341 aagccgttcc ggtttgtccg accggcggct ttgccccaat atccccattt tggagacacc
     2401 ctatgttgcg tttgaccaca ttgaccctct gcaccgtcct cgccttgggc gcgtgttcgc
     2461 cgcaaaattc cgactctggc ccacaagcca aagaacaggc ggtttccgcc gcacaatccg
     2521 aaagcgcgtc cgttaccgtc aaaaccgcgc gcggcgacgt tcaaataccg caaaaccccg
     2581 aacgcatcgc cgtttacgat ttgggtatgc tcgacacctt gagcaaactg ggcgtgaaaa
     2641 ccggtttgtc cgtcgataaa aaccgcctgc cgtatttaga ggaatatttc aaaacgacca
     2701 aacctgccgg aactttgttc gagccggatt acgaaacgct caatgcctac aagccgcagc
     2761 tcatcatcat cggcagccgc gcagccaagg cgtttgacaa attgaacgaa atcgcgccga
     2821 ccatcgagat gaccgccgat accgccaacc tcaaagaaag tgccaaagag cgcatcgacg
     2881 cgctggcgca aatcttcggc aaacaggcgg aagccgacaa gctgaaggcg gaaatcgacg
     2941 cgtcttttga agcggcgaaa accgccgcgc aaggtaaggg caaaggtttg gtggttttgg
     3001 tcaacggcgg caagatgtcg gctttcggcc cgtcttcacg actgggcggc tggctgcaca
     3061 aagacatcgg cgttcccgct gtcgatgaat caattaaaga aggcagccac ggccagccta
     3121 tcagcttcga atacttgaaa gagaaaaatc ccgactggct gttcgtcctc gaccgcagcg
     3181 cggccatcgg cgaagagggt caggcggcga aagacgtgtt gaacaatccg ctggttgccg
     3241 aaacgaccgc atggaaaaaa ggacaggtcg tttacctcgt tcccgaaact tatttggcag
     3301 ccggcggcgc gcaagagctg ctgaacgcga gcaaacaggt tgcggatgcg ttcaacgccg
     3361 cgaaataatg aaacggcggc attcgatgcc gtctgaaaca cggatgcaaa ccgcttcctg
     3421 tgtttcagac ggcattgccc gatacgggcg ttatcgcggg aatggcggca agcgttttga
     3481 ttggaacttg ccccatcctc tgcccgtccg gaggtttcaa acaaggcttt ccgctccgac
     3541 ggttcggact gccttgtttg aatctcctac gccgtaacgc ttttcccttc tgtttatgac
     3601 tgccaaacct ttttccctca acctggccaa cctcctgctg ccggcggtgt tgtttgccgt
     3661 cagcctgtcg gtcggcgttg ccgatttccg ctggtcggat gtgttttcgc tgtccgacag
     3721 ccaacaggtc atgttcatca gccgcctgcc gcgcacgttt gcgattgtgt tgacgggcgc
     3781 gtcgatggcg gtggcgggga tgattatgca gattctgatg cgcaaccgtt ttgtcgagcc
     3841 ttctatggcg ggtgcgggcc aaagtgcggc tttgggtttg cttctgatgt ccctgctgct
     3901 gcctgccgcg ccgctgccgg tcaaaatgtc ggttgccgcc gttgccgcgc tgatcgggat
     3961 gttggtcttt atgctgctga tccgccgcct gccgccgacg gcgcaactga tggtgccgct
     4021 ggtggggatt attttcggcg gcgtggttga ggcggtggcg acgtttatcg cgtataaaaa
     4081 cgaaatgctg caaatgctcg gcgtgtggca gcagggcgat ttttcgagcg tgctgctggg
     4141 gcggtacgag ctgctttgga ttacgggcgg tttggcggtg tttgcctacc tgattgccga
     4201 ccggctgacg attttggggc tgggcgaaac ggtgagcgtg aatttgggtt tgaaccggac
     4261 ggcggtgttg tggtcgggtt tgattattgt ggcactgatt acatcgctgg tcattgtaac
     4321 ggtcggcaat attccgttta tcgggctggt cgtgccgaat atcgtcagcc gcctgacggg
     4381 cgacaggctg cgccaaagcc tgcctgcggt cgccctcttg ggcgcgtctt tggttttatt
     4441 gtgcgacatt atcggacgcg tgattgtgtt tccgtttgaa attccggtct ctacggtttt
     4501 tggtgtgttg ggtacggctt tgtttttgtg gcttttatta aggaaacctg cccatgccgt
     4561 ctgaaaaaaa tatcggtttt atggcaggaa gcagccgtcc gttgcgggtc gcctttgcgc
     4621 tgttgctggt ttcctgcatc ctgtttatga cggtcaacgt caaaggcgat tgggactttg
     4681 tcttgcacct gcgcctgacc aagcttgccg cgctgctgat ggtcgcctat gcggtcggcg
     4741 tgtccactca actcttccaa acgctgacca acaacccgat tctgacccct tcgattttgg
     4801 gtttcgattc gctgtatgtg tttttgcaga ccttgctggt gtttacgttc ggcggcgtgg
     4861 gctatacatc cctgccgttg acgggcaaat tcgacttcga actggtcatc atgatgggcg
     4921 gctcgctgct gctgttttac acgctcatcc gtcagggcgg gcgcgatttg ccgcgcatga
     4981 ttttaatcgg cgtgattttc gggattttgt tccgcagcct ttcctcgctg ctttcgcgca
     5041 tgatagaccc cgaagaattt accgccgcgc aggcgaatat gtttgccgga ttcaataccg
     5101 tccgcagcga gcttttgggc ataggcgcgc tgattctgct cgtcagcgcg gcggtcgttt
     5161 ggcgcgaacg ctaccgctcg gacgtacacc ttttggggcg cgaccaagcc gtcaatttgg
     5221 gcatcagcta cacgcgcaac accttatgga tactgctttg gattgccgca ttggtggcga
     5281 cggcgaccgc cgtggtcggc ccggtaagct ttttcgggct tctcgctgcc tcgcttgcca
     5341 accacttttc cccgtccgtg cgccattccg tccgcctgcc gatgacggtt tgcgtcggcg
     5401 gcatcctctt ggtcggcgga caaaccgtat tcgaacactt cttgggcatg aaggcggtat
     5461 taagcgtggt ggtcgaattt gcgggcggac tcgttttcct ctatctcgtt ttaaaacaca
     5521 aaaaatgacg gatgccgtct gaacggcagc ccgccccgaa aggacaaacc atatgacgca
     5581 agaacgttta cccgaatttt tcgaccgcgc cccgacgctg accgtacaag acccgcttgc
     5641 cgcattcctc ggcgcggccg aaaacggcat cctcacttac cgctacgccg atgccgtgcg
     5701 cctgtgcgga cattcctgcc cgaccgtcgc gggcgcgtac ctgatggtta tcaaaggcct
     5761 gaaagcactt tacggcgaag agctgcccga acgcggcggc atcgaagccg ccatgcaggg
     5821 aacgcgcgac gaaggcacgg tcggcgtaac cgcgtccgtc gtccaactcc ttaccggcgc
     5881 agcccccgaa accggcttcg gaggcatcgg aatgcaggga cgcttcgccc gccgcaacct
     5941 cttatccttt ggtgcaggcg aaatcaacgg cacactgacc ctgcgccgca aagacaacgg
     6001 caaaaccgtc gccgtcagcc tcaacgccgc cctgcaaccc tttgcacctc aaatgcgcga
     6061 catcatgccc aaagccgtcg gcggcagcgc aagcgcaaaa gaactcgaac gcttcggaca
     6121 actttggcag gcacgcgtta aagcattttt gaccgaatcg gcggacgacc cgcagttcgt
     6181 catcgtccgc gaactgtgaa cgttcagacg gcattccgaa ttccaaatgc cgtccgaacc
     6241 cccgccaaac aacaacaaac ctacgcccga caagcatccg ccatgattac catccgcaac
     6301 gtcagctacc gcatcggcac acaccccatc ctcgacaacg tcagcctcga catccccgaa
     6361 ggcggcatta ccgcgctaat cggccccaac ggtgcgggca aatccaccct gttttccttt
     6421 atggcgcggc tgcgcccgct tgaaagcggc agcatcgcct accgaggcaa aaaccttgcc
     6481 ggcaccccca ccgccgaact cgcccgcacg ctctccatcc tcacccaaga aaacagcatc
     6541 atgagccgca tcaccgtgcg cgacctgctg atgttcggcc gttaccccta ccatcaaggc
     6601 agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt ccacctgcag
     6661 gacctctccg accgctacct gaccgaactc tccggcggac aacgccaacg cgccatgatt
     6721 gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct gaacaacctc
     6781 gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgca cgaacacaaa
     6841 cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc cgaccacgtc
     6901 gtcgccatga aaaacggcaa agccgccctg accggcacac ccgaagaagt gttcaccgta
     6961 cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga aggcaaaaaa
     7021 ctcatcgtcc accacatctg aagctcaaaa agaaaggggc tgtactagat tatccctaaa
     7081 ttccacaccg atcccgcagg atttttagct gccggggcgg tgtgccgaag ttaagtcgaa
     7141 attcgcattc cctcaagaac ggcgggaaag gtttgcg
//
LOCUS       DAXFHF010000081         7028 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXFHF010000081 DAXFHF010000000
VERSION     DAXFHF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.081"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(18..1784)
                     /locus_tag="ACFIRM_001848"
     CDS             complement(18..1784)
                     /locus_tag="ACFIRM_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC3029417.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGQKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(1777..2650)
                     /locus_tag="ACFIRM_001849"
                     /pseudo
     CDS             complement(1777..2650)
                     /locus_tag="ACFIRM_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2687..2971)
                     /locus_tag="ACFIRM_001850"
     CDS             complement(2687..2971)
                     /locus_tag="ACFIRM_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC3029418.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2958..3188)
                     /locus_tag="ACFIRM_001851"
     CDS             complement(2958..3188)
                     /locus_tag="ACFIRM_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC3029419.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKPGLKEDADGDE"
     gene            3460..3840
                     /locus_tag="ACFIRM_001852"
     CDS             3460..3840
                     /locus_tag="ACFIRM_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3029420.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4074..5114
                     /locus_tag="ACFIRM_001853"
     CDS             4074..5114
                     /locus_tag="ACFIRM_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC3029421.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTAYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5111..5485
                     /locus_tag="ACFIRM_001854"
     CDS             5111..5485
                     /locus_tag="ACFIRM_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029422.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5482..6120
                     /locus_tag="ACFIRM_001855"
     CDS             5482..6120
                     /locus_tag="ACFIRM_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029423.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6240..6527
                     /locus_tag="ACFIRM_001856"
     CDS             6240..6527
                     /locus_tag="ACFIRM_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029424.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6601..6822
                     /locus_tag="ACFIRM_001857"
     CDS             6601..6822
                     /locus_tag="ACFIRM_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029425.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6815..7024
                     /locus_tag="ACFIRM_001858"
     CDS             6815..7024
                     /locus_tag="ACFIRM_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC3029426.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYSQLKTK"
BASE COUNT         1738 a         1777 c         1638 g         1875 t
ORIGIN      
        1 ctgttttcgg ttgaaaccta accggttttc aaaagtttca gacggcatct tgtccgggtg
       61 cgttcaaacg cctcttccag cctgccctcg gtcacacctt ccgggtagtg tttgaggatg
      121 accgacactg cctgtttcca gtcgccgccg tctgcctcga cgcggggttt taagcgtttg
      181 gcgatttcga ccttgctcaa tacctgctcg gcgacctcca tcttgttgta atccatctgt
      241 tgcccctgtt tgggcataaa gagcgtattg cttgcggcaa gcgtatcttc ttgatgtttg
      301 tacgggtcga ttttgccgcc gaacgggact gccttgcctt tgcgtttggc ggctgccgcc
      361 tccagcgttt ccgcacccat cgccagcttg tccagctctt tgcgatgccg ttgcgcgtct
      421 gtatcggccg gggctttgta ttccgccccg atgaccgcgg catcagcctt gaagcccatc
      481 tcgtcaaaaa ctacttcggg tacggatacc caaacctcgt taccctccgc gtcataagtg
      541 gcgacccgcg ccccgtttgc ctcccaagga ttcttaccga ccaaaacttt ctgaccgacc
      601 aaaatcccct tgatgccttt tacgctatat acccgtccgc caaagcggat ttccaaatcc
      661 gccgagactt tcgcctcttt cggcgcgctg acggcaagct ctcggcaata ttcggcaggc
      721 ggcggcagga tgagctgctc gggtttgatt ttgttccacg cctgatagcg ggtcatgccg
      781 tggcggctgt gtttttgcgt accgttgtag taacgcatcc agcgttccga taaagcattg
      841 agctggtcga tgtcgtgtac ctcggtaaag cgcaacccgc tctcaaatgc cgtttccaca
      901 atatcgttgg ctttttccac ttgccccttg gcacgcggat tgcccggctt gttgatttgc
      961 acatgcacat ccaagggctt gcacaaattt ttaaacgccg ccgaagtatt cgcgctgccc
     1021 gggtcaagca tgaccatgcg cggtacggtc cggaacgggt ctttgccgat gtcttttttc
     1081 gcctgcatca tgtagatgaa aaaatcacag aggttcgcgc tggtttcgcc gccgaagtaa
     1141 taacgcaccg aaatcgtgcc ggaggcatgg tctgtccccg tgtaccgcca aacgcggtcg
     1201 ttttcgattt tgacgacgtt tttcggcttg tttttataaa actcctcttc cttcatcacc
     1261 cgcagccccg tatccttgcc ctgacgcggc aggaaataca aaacgcacaa actcgggtcg
     1321 atttgccaac aatggttcgg gtgttccgat ttcatgcggc tgacggggtc gggctgcaaa
     1381 agctggtcgg aatgcagctt gtattcccgt aaagcccggg taatggtgtt ttcagaaagg
     1441 gggatgactt ccccggtttc cccgtcaatc cgcgccgcct cgattttccc gttggcgcgc
     1501 agcatttcca ccgcctgccg caccggcatc aaccgcttgc cgttgcgcct catcgcctcc
     1561 gccaaaaccg ccgaaatcaa tttggcttct tccggtttaa gctccgtctt gcccgcatcg
     1621 ctgcgccgtt tgcgcgtcgg cttgacgctg accgcctcca gcttgcggta tagcgtggca
     1681 aggctgatgc ccaattcctg cgcctgctgc ttaagatatg cagagcgtgc gccgcgcccc
     1741 attgcttccg cctgattttc gactgcttta agacgctcaa tcattgccgg attcatcgcc
     1801 ttctcccgtt tcaccgccca accattccgg cacattgtct gccggtgctt cggtcggtag
     1861 ggcatagctt tcgcgcagtt gctcgcagtc caaaataatt tgattgagcg tgccgaccat
     1921 cttcgcgcgg tggtcaaatc catgcgcctc gccgtgcgcc gccatctgct cgaacatatc
     1981 gcgcaatcgg ctgatttgcg agcggatacc gacttccaag ctgccaagct gcatcgtcag
     2041 ctcgatgccc acatccgcag gtttaggctc ttttacacct gtctgctttt tcgacagctt
     2101 ttccgccagc tcatcgacct ttttattttt atcggcaatc accttatctt tcgcttccgc
     2161 cgtttcacgg ctttcgcgca gggcgacgcg cagctcctta atagtcatac ggtcgacatc
     2221 gtcaaaagtg ttgccgttga cctcaccgcc ttcggcaagc tccaacaaag tgtcgtcgtc
     2281 ttcgaccagc agctcgagca gtttggattt gcccaacgtc atcagcttag gctgcgcttg
     2341 tttcattttc gggtcgataa aacggagggt ggcattcatc agtctttggg attcccgccg
     2401 tccgaggcca actctttttc agcgatttcg gcaaaccgcc catgcggcgt atgctctttg
     2461 atgatgatca gcgcgcgtcc cggctcaaac atcccttcca tcgtttggcg tccgcaaatc
     2521 gtccgcgctc aatccatacc gtctcgttgt aggcctcgcc gcctgaaaac ttgtccataa
     2581 cctccatact gtggatagcc aattcgtttg ccgttacgcc gaccgtatgt cctaaaattt
     2641 ctgttgccat ttcttttgct cctcaaatgc gacgacgtcg tcgcatttag taaattcggt
     2701 tttccaattc ttgcaagcgc gcattcagcc gctcttgctg ttttctgaac cgctctgcga
     2761 tttgtagggt tttgatgccg taggcgtagt tgccgttttc aagcttgatg accaatcccg
     2821 atgcaaccaa atcatcaata tccctgctga cttgtgatgg cgtcagcccc aggccgtctg
     2881 aaagctcctt attgctgatg ccgattaccg gatgcgcgtc catcgccaaa aacacccgca
     2941 acagccgctg cgccttttta ctcgtcgcca tccgcatcct ccttcagccc aggttttacc
     3001 gccgcttcgt ggcttttgcc gaaattgcct ttcagcttgc cgcgcaaggg gtgttccacc
     3061 gcggtgcgct ccaaattgaa atgtttcgcc cggtgcgtct tgcacacgcc gttgcgctta
     3121 aaccacacgg ccgtgctgcc gcgcgtttgc ggatagggaa taggcttgaa atttaaaggt
     3181 ttttccatat tatttatgcc tttcgtgtgg tataatgtgt ttagttttag gcctctttgc
     3241 acgataaaca tccggtctgt tgtgcagcag cagggtcttg gcttgtttta aaataaacaa
     3301 gctttttccc gtagccgggt gtatgtgatc catcatgagc tgtcgcgcat gagccttcaa
     3361 agtgttcatt ttttcgtcct ttttcgtgat gatttagggt gtttcatgtc ttgatgtgtg
     3421 aattatgaga aaaatatttc tcttttcaag gaaatttaag tggaaaaaac ttctcttttt
     3481 ggtaagcgat tgaaagaaga aagaattaaa ttaggcttga accaagcaga agctgctgaa
     3541 aaatgtggtt tttctcgtga gatgtgggga aagtgggagc gtggtgaaaa tcgcccatca
     3601 agtgaaaaat tattctcttt ttctaaaatc ggcatcgata ttgattacgt catgcacggc
     3661 agacgcggcg aaacagccgc catgccgtct gaatccttga gtgctgaaga aaaagaactg
     3721 cttgccctgt tccgtcagct cggcagcggc agccgcaaag agcttgctga ctacgccgcc
     3781 tttaagctgg tggtagagaa aaaagcccaa actgcgcttg gtaaagtgag caacggataa
     3841 aaaatgccgt ctgaagattt cagacggcat ttgggacact ctgacagtcg ggctgctccg
     3901 gtgttctgaa acagatatta agcaatagga gcggccgtgc attttaaagc gtattaaaaa
     3961 ccaccccgat attcaggtca tcggccaagt ggttcagatt tcaaaagacc ttaattaact
     4021 tacaatagga aaaatgatga cagaaacgaa ttactctttt gaagggctgg taattgaaaa
     4081 aaattattac ccatcgtatt tatccgaaaa atgaaaatag agagcgtgta gaacctaaaa
     4141 tcagtacaca tttgattgat ttgcctatta cggcaagaag aacattggaa accaggctga
     4201 ccaaagcttt gggtaataaa tctcatggca ttgaaatgtc tgttgtcaat acggctgaaa
     4261 atagtttttt tcagatagcg gcagccatac aactcaaaga ggaagcagag tttattgagg
     4321 attccgcaca atttgcccac atgttgaccg atgcacaact gaatacaaat gcaccgggtg
     4381 gtattttgtt ggttttaaaa ggtagggttg gagataccgg taagccgttt ttatgtgtaa
     4441 ttaaggctga acctcaagat gggttccgaa ccaaagaaga ggatgacttt atcacgattg
     4501 aattcttaga agaattatta ctgaccgatt cagcaagatt attcaagata ggttttttgg
     4561 tggctgaaac agtaaggccg ctagagcaaa tacaatctgg gaattatcga gcttttttgt
     4621 atgaccatct gatgacacaa acggaaacta gaccggcagc ttcctatttc tatcaagtat
     4681 tcttgggtat gagtatagct gcttcttccc gtaaattgac gcaggatttt tttgagtgga
     4741 cacgcaattt tatcgataac tctgatttaa gtgatgatgc aaaattagat gcgcatgaag
     4801 cattgcgcgt tacattgaaa agtgcggaag caaccattag tgtaaataat tttgcccaaa
     4861 atcatttacc tcaagaaaaa cgaacagctt atacagaatt tatggtggaa aaggattttc
     4921 ctcaaaatgc cgtaagtaaa gatattgaat atattaaaac tcgtttacgc aaacggaggt
     4981 cttacggatt tagtaacggt gtagttatct tgactcctcc cgagcatact caggactata
     5041 tggaaattgc gccaacggaa gatggggaat atactgttgt cctaattaaa ggacagttac
     5101 aacaacaaaa atgagtgata ttgatgattt caaaagctat ttagaaagcc accaagcggc
     5161 attttccgct tggggtagat ttgtggctga agaaattcaa aatcaattat ccaatgtcat
     5221 ttctcctgtt ccggttgcca attttctaaa aattgaagca aagcctggag tcaaagagat
     5281 ttcttcagct ctagccaaga ttggccgaaa aaattacact tcacctcaaa ctcaaatgac
     5341 tgacttggtc ggcgtacgtt ttgtggcatt gcttgcggaa catattcaaa tagtttgtga
     5401 aatcattgaa tcctcatctc agtggaatgc taaagtttcg aaagattttg cagatgaaat
     5461 tcagcaaaat cccaaagcaa tttgactata caatccaaac attacgagat tcgtcctaaa
     5521 caagcattca ttacacccga aaatgtcagc atccctgctg atttgtgctg tgaagttcag
     5581 gtgcgttctt tactccaaca tgcttatgca gagctggttc atgacaatat atacaagcct
     5641 gacggcaatg ttccaaagca agcagagcgc gaagtcgcga agagcatggc tttgatggaa
     5701 actacagatg acttatttag tcgtactctg gcaattttga aagaagcaaa tcaaccacaa
     5761 gaagagcttc ttccccaatt atcacagctt tatcaaaagg aaatagggct tgttcctgaa
     5821 gttgataaaa aaaccaatat gatttttcta gaaacatttc aatccagcat ttcccaatca
     5881 agtattcttt ctgacatccg ttcacttttg aatgagaaga aatatattgc caagcggata
     5941 aaagaaaatg ctgaagaaat gtattttttc agtcagccag cagctttgtt ggtctattgg
     6001 ctgattgaga aagttggtgc agatgaagta tggaaaaaat ggcctttgcc tgcatacaac
     6061 aaaaacctga aattcatttg tactgatttg gataagcaac catcccatga actcttttaa
     6121 ccccctttaa aagcccattc agacggcctt tcctaattat gccttaatca aacgcggcat
     6181 agcggcagtt ccgcagctgg cggaaatcaa aaaatccgca aatcaggaag ggggcggcaa
     6241 tgattgaatt tgtccgagcc aaaaaacggc tgctttgggc atttgtactt ttgcttgcgt
     6301 gggcgtgcgg ctaccggtac gctgccgaca aggccgaagc ggaacaaacc gccctgattg
     6361 ccgcctatcg gcattcttct atggttgcgg cggaacaata cgccttgcag cttaaaaaag
     6421 cgcaggacga aaggcagcgg tggtacgact tttcccaaaa acaaagcacg gatttggcgg
     6481 ccgccctgag cgaattggac aaaacgcgca acaccttaca ggagtaaacc cgtgaagcgg
     6541 cagacaaaga cggcaaccgt tttaacggcc ttggcacgaa cagcctgcac ctctacaacc
     6601 gtgccttcgg ataccccgat taaaaccgtt gccgtcgcgg aaattccgcc cgttccttcc
     6661 ggactgctgg tcgaatacga acgccccgag cgtccggccg gcggctctcc cgaacaactt
     6721 ttaaaccacg ccgtccgcta tggcggatac taccgaaagc tcgaaatcca aattgagggc
     6781 tggcagaact ggcacacgaa aggccgtctg aaacatgact gattttgccg accgcgcatc
     6841 cgaacgcgaa gccgaatttc tggtggaagc cttggcgaaa caccaaccgc cgtctgaaaa
     6901 caccgccggc ttcagccatt gcgaagactg cggcgacccg ataccggaag caaagcgaaa
     6961 agccgtccga ggctgtacgc gctgtgttgt ctgccacgaa tatagtcaat taaaaacaaa
     7021 atagtaca
//
LOCUS       DAXFHF010000082         6860 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXFHF010000082 DAXFHF010000000
VERSION     DAXFHF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.082"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(956..1639)
                     /locus_tag="ACFIRM_001859"
     CDS             complement(956..1639)
                     /locus_tag="ACFIRM_001859"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC3029427.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1788..4091
                     /gene="parC"
                     /locus_tag="ACFIRM_001860"
     CDS             1788..4091
                     /gene="parC"
                     /locus_tag="ACFIRM_001860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC3029428.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRIVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKAIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNIFLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPISSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4111..5835
                     /locus_tag="ACFIRM_001861"
     CDS             4111..5835
                     /locus_tag="ACFIRM_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC3029429.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSHTFEHHIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            5935..6687
                     /locus_tag="ACFIRM_001862"
     CDS             5935..6687
                     /locus_tag="ACFIRM_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3029430.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1645 a         2045 c         1684 g         1486 t
ORIGIN      
        1 actattttag tagttttacc attagaaaaa ggatatgaat ggaattgcct tggggtagaa
       61 gtactatccg ctgctcaatc tctagcaaat atctattgca acaaggttcg cctcatagat
      121 ggtcatgttt gggaggaata aatgaaaaag cgtagttggt aatttttagt tgcgcagggt
      181 gggcttcagc ccaccgtttt ttcttcattt tgggttgttg attggtgggc taaagcccac
      241 cctacgatta ctaaaatccc gtcattcccg cgtagagcgc atcatataag gcgggaatcc
      301 agacctttcg gtgcgaaaac ttatcggata aaacggtttc ccctgcatcc tggattccca
      361 ctttcgtggg aatgacggga tataggtttc cctacggacg tgttcagatt cctgccttcg
      421 cgggaataac ggcggagcag tttctgcttt tccaataaat gaccgtaact taaaatcccg
      481 tcatccccgc gaaagcaaaa atcctgcctg tcggatttcg gttttttcgg gtgtttcggg
      541 aaactgatga atcgtcattc ccgcgcaggc gggaatccag aacgcgggac ggcggcaata
      601 ttcaaaggtt gtctgaaaat tcagaggttc cagattcccg ccttcgcggg aatgacagcg
      661 gagcagtttc tgcttttccg ataaatgacc gcaacctaaa acccatcctt cacacgaaaa
      721 cagaaaaaca aaaacctaaa atcccgtcat tcccgcaata acagttgcgt aattgcgtag
      781 ggtgggcttc agcccaccgt ttcttcattt ttttagtgga ctgaagccca ccctgcggtt
      841 actgtttctt ctttttcgat tattaattgg caggctgaag ccctccctac gtttaccgtc
      901 aaaaatgccg tctgaaatgc tcaagccctc agacggcatt ttttatttcg cacccttaca
      961 ggtaaaattt ctcgatgact ttcaggttgt cgtctaattt gtacaccagc ggctggccgg
     1021 tcgggatttc caagcccatg atgtcttcgt cggaaatgcc ctcgatgtgt tttgccagcg
     1081 cgcgcaggga gttgccgtgc gccgctacca agacgcgttt gccgctcaaa atcgcggggg
     1141 cgatttggtc ttcccaaaac ggtaatacgc gctccagcgt tactttcagg ttttcgccgt
     1201 cgggtacgac atcggcaggc agatgggcat agcgacggtc tttgtgtgcg gaaaacgcgt
     1261 cgtctttgtc caaaagcggc ggcagggtgt cgtagctgcg ccgccagatg cggacttgct
     1321 cgtcgccgta tttttcggcg gtttgttttt tgtccaaacc ttgcagtcgg ccgtagtggc
     1381 gttcgttcag cctccatgtt ttgatttgcg gtacgaacag ttggtcggat tcttccaaaa
     1441 cgatgttgca ggtcttaatc gcgcgggtca ggacggatgt gaaggcgatg tcgaactcat
     1501 agccgttttc tttcagtttt ttaccggcgg cggcggcctc ggcaagcccc tgctcgctca
     1561 gcttcacgtc gcgccagcct gtaaacaggt ttttcgcgtt ccattcgctt tgtccgtggc
     1621 ggataaatac caattccata tcgtctccaa tgtgtgaaag tgggaaagct ttatttataa
     1681 catattttca catttcccgt atttgattca gattcaggca cggcgcccac tatggtttgc
     1741 cattttgatt tacaataatg tcctttgctt tacattccgc atacacaatg aatacgcaac
     1801 cgcacgcttc ccataccgat tccaacacgc tgatgctcgg ccgatacgcc gaacgcgcct
     1861 atctcgaata cgccatgagc gtggtcaaag gccgcgcgct gcctgaagtt tcagacggcc
     1921 aaaagcccgt gcagcggcgc attttgtttg ccatgcgcga tatgggtttg acggcggggg
     1981 cgaagccggt gaaatcggcg cgcgtggtcg gcgagatttt gggtaaatac catccgcacg
     2041 gcgacagttc cgcctatgag gcgatggtgc gcatggctca ggattttacc ttgcgctatc
     2101 ccttaatcga cggcatcggc aacttcggtt cgcgcgacgg cgacggggcg gcggcgatgc
     2161 gttacaccga agcgcggctg acgccgattg cggaattgct gttgtccgaa atcaatcagg
     2221 ggacggtgga ttttatgccg aactacgacg gcgcgtttga cgagccgctg caccttcccg
     2281 cccgcttgcc tatggtgttg ctcaacggcg cgtcgggcat cgcggtgggt atggcgaccg
     2341 agattccgtc gcacaatttg aacgaagtca cgcaggcggc gattgcactg ttgaagaaac
     2401 cgacgctgga aaccgccgac ctgatgcaat atattcctgc tcccgatttt gccggcggcg
     2461 gtcaaatcat cacgccggcg gacgaattgc gccgtattta cgaaaccggc aagggcagcg
     2521 tgcgcgtgcg tgcgcgttat gaaatcgaga aattggcgcg cggacagtgg cgcgtcatcg
     2581 taaccgaact gccgccgaac gccaattccg ccaaaatcct tgccgaaatc gaagagcaaa
     2641 ccaacccgaa accgaaagcg ggtaaaaagc agctcaacca agaccggctc aataccaaaa
     2701 agctgatgct ggatttaatc gaccgcgtgc gcgacgagtc cgacggcgaa catcccgtgc
     2761 gccttgtatt tgaaccgaaa tccagccgca tcgataccga taccttcatc aacacgctga
     2821 tggcgcaaac ttcgctggaa ggcaatgtgt ccatgaactt ggtgatgatg ggtttggaca
     2881 accgccccgc gcagaaaaac ctgaaaacga ttttgcagga atggctggat ttccgcatcg
     2941 ttaccgtaac acgccgtctg aaattccgtt taaaccaagt ggaaaaacgg ctgcacatcc
     3001 tcgaaggccg tctgaaagtc tttctgcaca tcgacgaagt gattaaagcc atccgcgaat
     3061 cggacgaccc gaaagccgat ttgatggcgg cattcgggct gaccgaaatc caggccgaag
     3121 acattttgga aatccgcctg cgtcagctgg cgcgtttgga aggtttcaaa ctcgaaaaag
     3181 aattgaacga attgcgcgaa gaacaaggcc gtctgaatat ctttttgggc gacgaaaacg
     3241 aaaaacgcaa gctgattatc aaagagatgc aggcggacat gaagcagttc ggcgacgcgc
     3301 gccgcacgct ggtggaagag gccggacgcg ccgtgctgac acaaaccgcc gccgacgaac
     3361 ccatcacgct gattttgtcg gaaaaaggct ggatacgcag ccgtgccgga cataatctcg
     3421 atttgagcca aaccgcgttc aaagaaggcg accgcctcaa acaaaccctt gaaggccgca
     3481 ctgttttacc cgtcgtcatc ctcgattcat cgggcagaac ctactcgatc gatgccgccg
     3541 aaatccccgg cggacgcggc gacggcgtac cgatttcctc cttaatcgag ttgcaaaacg
     3601 gcgcgaaacc cgtcgcgatg ttgacaggat tgccggaaca acattattta ttatcaagca
     3661 gcggcggcta cggctttatc gccaagctgg gcgatatggt cggacgcgtg aaagcgggca
     3721 aagtggtgat gaccgcagac agcggcgaaa ccgtcctgcc gccggttgcc gtctatgcct
     3781 cctcgttcat caaccccgac tgcaaaatca ttgcagccac cagtcaaaac cgcgccctcg
     3841 ccttccccat cggcgaattg aaaattatgg cgaaaggcaa aggactgcaa atcatcggat
     3901 taaacgccgg cgaatcgatg acgcataccg ccgtttcttc cgagccggaa atcctgattg
     3961 aaagcgaagg caggcgcggc gcggcgcaca aagaccgcct ccccgtcgcc ctgattgagg
     4021 caaaacgcgg caaaaaaggc agactgttgc ccatatcggg cagcctgaaa cagctttctt
     4081 cccccaaata aacccggttc cgcacatatt atggtgattt ccaacccccg cgaacttgaa
     4141 aaactcaaag accggattcc caatctgatc aacatcatcc gcgtcgccat cgttttcccg
     4201 ctgatgatta tgcacatcct cgggctggaa accggcagcc gtgcgaacct gcacgcctca
     4261 tggacggcgt gggcgtttta cttgtggctt gccattgcct gctggctgat tttcttttcc
     4321 accctcaacc cgcaatggca atggcaatgg caggctttga gaataccgag tttcagcgcc
     4381 gtggccgaca tcacgatgat cggcgtgctg acctacctgt tcggcggcat cgattcgggg
     4441 ttcggcatcc tgatcctgcc cttcgtgggc agctcctgcc tactcagtta cgggcgttat
     4501 cccctgctct atgccagcta tgcatccatc ctgctgatat tcaacgccct tgcagacagc
     4561 aatatcaata tgtatccgct gattctggat gcaaaaaccg tcaccaatac cttcgtcgtc
     4621 gtagccggct cctatttcgt cgctatgatt gcctcgctgt cggtcagata catcgaccgt
     4681 gccggcaaac ttgcccatga aaaccacgtc gcctaccgcc gtatcagggg cttgaaccaa
     4741 atcgtgctca accgcgttca ggaagcggtc gtcgtcatca acgtcgagca tcagaccata
     4801 ctgttcaata aaaaggcaaa agatctgctc cccatgcttg aaatcggaca gcatacctcc
     4861 ctgttcgacc ctgtcgccat cttatgggat aaaacatcct cacacacctt cgaacaccat
     4921 atcgacacgc ccgaactgac cgcccgcatc cgcgccgtgc cgatgaacaa aaagcagaac
     4981 aagctgctca tcctctatat ccgcccgcaa agcgaaattc aggcggaagc cctgtccgtc
     5041 aaacttgccg cgctcggaca actgaccgcc aaccttgccc acgaaatccg caacccgatg
     5101 tccgccatcc gccacgccga cggcggcact ttatttttgg acgaagtggc cgacctgccc
     5161 ctttccatgc aggtcaaact cttgcgcgcg attcaagaaa aagccgtgcg ccgtatcggc
     5221 gacgcgaccg agcagcccgt cgatgtccgc atcgtctgcg ccacccacaa aaacctcgaa
     5281 gcccttgtcg aaagcggcgc attccgccaa gacctgtatt accgtctcaa tgtcgtcagc
     5341 ctcaatatgc cgtccctgcg tgaaatgcgc gaaaatttga agctgcccac cccctacctc
     5401 ctgtacaaac acagccacaa caaccggccc tacacactct cccccgccgc gcaacagatg
     5461 ctcctgaatt acagttatcc gggcaatttc cgcgaactcg aaaacatcct cgaacgcgcc
     5521 gtcgccctgt gcgtcggata tacagtgcaa atcgacgacc tgcaaatcca agatgtgcac
     5581 cacaaacccg tccggacgga aaccgccgtc cccgttgccg ataccttgcc gtctgaaaca
     5641 gccgccgcac cgtcccgcct tctcccgttc gaccccgata ccatgcagat acaggactat
     5701 ctcgacaaaa tcgaacgcga catcatcgga caagtcctca aacaaaccga aggcaaccgc
     5761 acgcaagccg ccaaacgctt gggcatcagc ttccgctcta tgcgctaccg tatggaacgc
     5821 ctcaacatag actgacaaac ggcatccgca ccatctccgc ccacccgaaa aaatgccgtc
     5881 tgaaacggca cgggaaagcg ggttcgcccc acgaccgaac ggacacaata caccatgacc
     5941 gacatcctca ttgacgatac cgccaccgaa gccgtccgca ccctgatacg ggcattcccc
     6001 ctcgtgcccg tttcccaacc gcccgaacaa ggcagttacc tccttgctga acacgatacc
     6061 gtcagcctca ggcttgtcgg ggaaaaaagc aacgtcatcg tcgattttac ctccggcgcg
     6121 gcacaatacc ggcgcacaaa aggcggagga gaactcatcg ccaaagccgt caaccacacc
     6181 gcgcacccca ccgtatggga tgcaaccgca ggattggggc gcgacagctt cgtcctcgcc
     6241 tcgctcggac tgaccgttac cgccttcgag caacatcccg ccgtcgcctg cctgctttca
     6301 gacggcatcc gccgcgccct cctcaatccc gaaacgcaag acaccgccgc gcgcatcaac
     6361 ctccatttcg gcaatgccgc cgaacaaatg cctgcccttg ttaaaacaca aggcaaaccc
     6421 gacatcgttt atctcgaccc gatgtatccc gaacgccgca aaagtgccgc cgtcaaaaaa
     6481 gaaatggctt atttccaccg gcttgtcggc gaagcgcaag atgaggtcgt cctccttcat
     6541 accgcacgcc aaacggcaaa aaaacgcgtc gtcgtcaaac gcccgcgcct cggcgaacac
     6601 cttgccggac aagcccccgc ctaccaatac accggcaaaa gcacccgctt cgacgtttac
     6661 ctgccctacg gagcggacaa gggataacgc ccataaaaca agacaccgaa aaatttgccg
     6721 ttcttatgca aacgagaaac cggtttttgc gtttcgactg ttttggataa gtcatcacac
     6781 cttaaagttt gtcattccca cggaagtggg aatccgattc gttcagtttt agttgttttc
     6841 aagtttcagg caacttccaa
//
LOCUS       DAXFHF010000083         6302 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXFHF010000083 DAXFHF010000000
VERSION     DAXFHF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.083"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            143..811
                     /locus_tag="ACFIRM_001863"
     CDS             143..811
                     /locus_tag="ACFIRM_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029431.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            783..1268
                     /locus_tag="ACFIRM_001864"
     CDS             783..1268
                     /locus_tag="ACFIRM_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFC3029432.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            1477..2199
                     /gene="lpxH"
                     /locus_tag="ACFIRM_001865"
     CDS             1477..2199
                     /gene="lpxH"
                     /locus_tag="ACFIRM_001865"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC3029433.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            2468..4054
                     /locus_tag="ACFIRM_001866"
     CDS             2468..4054
                     /locus_tag="ACFIRM_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC3029434.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(4220..4423)
                     /locus_tag="ACFIRM_001867"
     CDS             complement(4220..4423)
                     /locus_tag="ACFIRM_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC3029435.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            4539..5036
                     /locus_tag="ACFIRM_001868"
     CDS             4539..5036
                     /locus_tag="ACFIRM_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC3029436.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            5118..>6302
                     /locus_tag="ACFIRM_001869"
     CDS             5118..>6302
                     /locus_tag="ACFIRM_001869"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC3029437.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVK"
BASE COUNT         1522 a         1760 c         1573 g         1447 t
ORIGIN      
        1 atagtctaac ccgcctttct ttaatcgctc tacttaattt ccacttcttt ttatcctgtt
       61 cgatcttttg cggctgcacc tcaagaaata aactattcgt tatcaggcgg taaagtttac
      121 gcagataagt tcagtatcgc cattggggtt gaggcaaaag ctcaggggga taataccaaa
      181 cttagcaacc tcaatcggca catctgccga agcaaatgca cccggggcat tggctttagg
      241 gggaaattct gaagcatcga aaaaatttag tatagcagaa ggctatctgg catctagtga
      301 tggctatggt gcaatagcga ttggttctgc cgccaagata aagcaattgg aaaaaggcac
      361 aataaatcat attgtgggaa acgataataa aggtctttat gtcgatgcag acggcaatgt
      421 aacaaagata accgtaagga ctgagtcgga aaaagatatt ctctcaagat acggtcaaac
      481 ttacggtgcg atggcactag ggtttagatc ttcttcacat aatctttttg ccagttcatt
      541 tggagcgttt tctacagcca cagctattga aagcttggca gtcggcgaca gcagccaatc
      601 aacgggctac cgcagtgcta cttttggcag tcacagcagg gctttggcag aagaaagttt
      661 ggcattaggt tatgaaactc gggcaaatgc ttatggttct gttgctttag gtgcagaatc
      721 tgtggcgaat gaagaaaata ccgtatcagt gggttccgat acattgaaac ggaaaatcgt
      781 taatgtcgcc gatggcacgg aagatttatg atgcagtaaa tgtccgccag ttgaaccgct
      841 taagcaaacg tacaaaccgc gtcggcgcaa gtgctgcggc gttggcttcg ttaaaacctg
      901 cacaattagg caaaaacgac aaattcgcct tttctttggg ctttggaagc tataaaaatg
      961 cccaagcggt ggcaatgggg gcggtgttta agcccgctga aaatgtgttg cttaatgtgg
     1021 cgggcagttt tgccggaccg gaccgggctt tcggcgcggg ggtttcttgg aaattcggcg
     1081 gcaaaccgac acctgcggtt gccgcacaaa acgcggcgca tcctgcaaaa gttttgcaat
     1141 tgcggcagga agtggcggcg ttgcgcgccc ggcaggcaga aaccgaccgc aaactgcaca
     1201 aacaggccga aatggaaaac gagctgcaac agttgcgccg cgcgttgtcc gaattgaaaa
     1261 aacattgaag caataaacgt aaggcgggac ggcggctctg cctttttccg atgccgcacc
     1321 cgcgcgatgc cgtctgaagc cttcagacgg catttttaac gcgcccgaac cccggttccg
     1381 ccaatgcccg atgcttggcg cggcatttgc cgtattgccc cgccgccggg cgcgttataa
     1441 tccgccacga catccgcccg atttggaaac ctgcctatgc gaccctccta tttcatttcc
     1501 gacctgcatt tgagcgaaaa gcaccccgaa ctgaccgaac tgctgctgcg ttttttacgt
     1561 tctgccgccg ccgggcaggc gcgggcggtt tatattttgg gcgatttgtt tgatttttgg
     1621 gtgggcgatg acgaagtttc cgagttgaat acttcggttg cgcgggaaat caggaaattg
     1681 tccgacaaag gcgttgccgt gttctttgtc aggggcaacc gcgatttcct aatcggtcgg
     1741 gatttttgcc ggcaggcggg tatgacgctg ctgccggatt actcggtttt ggacttgttc
     1801 ggcagcaaca ccctgatttg ccacggcgac acattgtgca ccgacgacaa ggcatacctg
     1861 cgtttccgcc gcatcgtgca ttgcaggcgg ctgcaaaaac tgttcctgat gctgcccctg
     1921 aagtggcgca cgcgccttgc cgccaaaatc aggcgtgtca gcaaaatgga aaaacaggtc
     1981 aagcccgccg atattatgga tgtcaatgcc gcctttaccg cgcggcaggt tcgcgccttt
     2041 aacgcggaaa ggctgataca cggacacacc caccgcgagc atatccatca cgaaaacggc
     2101 tttacccgca tcgttttggg cgactggcat aacgactatg cttcaatcct ccgcgtggac
     2161 ggggacggcg cggtattcgt gccgccggaa gaatgctgaa aatgccgtct gaagcctttc
     2221 gggcggcttt ttctacacgc ccctttccca cactccctcc gccgcatcat caccatcctt
     2281 aacccgcatt aatagccatt atcgataaaa tgcttgaccg attacataag attacgtaaa
     2341 gtgtgcaaac gcataataat tggtcttacc aatttatctt ttaaccaaaa tatcgggtgt
     2401 gcgggatttg aacgtctttc caaacctcga cgcgccttat gatgcaaatg cctaaaaagg
     2461 attcgttatg gcacttttcc tcagcatatt ccccatcgtc ctgctgattt ggctgatggt
     2521 gaaaaaaaac agtatgcctt cctacgtcgc actgccgatt accgcagtgc tgatttacgc
     2581 catcaaactt ttctacttcg gcgatgcggg aatgctgctc aacgccaccg ccgcttccgg
     2641 cctcgtcaaa acgctcacgc cgattaccgt gattttcggc gcgattatgt tcaaccgtat
     2701 gatggaaacc acgggctgca tcgatgtcat ccgcaaatgg ctggcgacca tcagcccgaa
     2761 ccccgtagcg caactgatga ttatcggctg ggcttttgcc tttatgattg aaggcgcatc
     2821 cggcttcggc acgcctgccg cgattgccgc gccgattctg atgagcttgg gcttcaatcc
     2881 gttgaaagtg gcgattttca ctttggtgat gaattccgtg ccggtatcgt tcggcgcggt
     2941 cggtacgccg acctggttcg gtttcgcacc gctgaacctg agtgccgaag acatcctcgc
     3001 catcggcagg cagaccggcg tgatgcactt cttcgcaggt ttcgtcatcc ctgtcatcgg
     3061 tttgggcttc atcgtgcctt ggtctgaaat ccgcaaaaac ttgggcttcg tcgccattgc
     3121 cgtcttctcc tgcaccattc cttatgccgc attggcgatg gtcaacgaag aattcccgtc
     3181 gctcgtcgcc ggcgcaatcg gcctgatggt gtccgtattc gccgccaacc aaggctgggg
     3241 cttgagcaaa gaccacgcca aagacccgaa tgccgaaaaa gtgccgttcg cccaagtcgc
     3301 caaagcactc gcccctttgg gtatgctgat cggcatgctg gtggttaccc gcatcaaaca
     3361 gctcggaatc aaaggcattt tgaccagcaa agaagaatgg ttcagcttcc aactgccgtt
     3421 tgatttgtcc aaaattaccg tcagcgactc cctgacgatt accttcggca atattttcgg
     3481 acaagatgtc agcgcgtctt accaaacgct gtacgtcccg gcttggattc cgttcgtgct
     3541 gaccgtttgg atttgcatcc tgctgtataa aaccaaattc aaagatgcct ggacgattta
     3601 tgccgtaacc ttcaatcaaa ccaaaaaacc gctgcttgcc ctgatgggtg cgctgattat
     3661 ggttcagctg atgctggtcg gcggcgacaa ttcgatggtg aaaatcatcg gtaaggaatt
     3721 tgccgcaatg gcgggcgaac actgggttta tttttcgccg tatctgggcg cgatcggtgc
     3781 gttcttctcc ggctccaaca ccgtgtccaa cctgaccttc ggcccgattc agcagcaaat
     3841 cgcccttgat accggcctgt ccgtgaccct gattctggcg ttgcagtccg tcggcggcgc
     3901 gatgggcaat atggtgtgcc tcaacaacat catcgccgta tgtaccgtat tggacgtgaa
     3961 aaattccgaa ggtgcgatta tcaagaaaac cgttatcccg atggcgattt acggcgtgat
     4021 tgccgtcgtc gcggcaatga ttttcttcct ctaggcgaaa cgccgccatc catagcggca
     4081 gcacgcgggg aacggaaacc gccgctcgtc cgggcggcgc gtcgggcaga atacccgtgc
     4141 cggaacagcc cgccccgcca aacaaatgcc gtctgaaacc ggaaaaggct tcagacggca
     4201 tttttatcgc cgtttgcatt caggcgtagt aatgatgcgg catcttgccg tcaaggcggt
     4261 agcgcgtgcc gcaatacggg caggcaacgc tgcccgattc tccttcgccc aaaggtaaaa
     4321 acaccctcgg atgcccgttc cactgctcgt tgtccggtcc cgagcaatac agcggcagat
     4381 tttccggcaa cacggaaatt tcctgcggat tcagattgtc catttgattt tcctttgcgt
     4441 ggttggcgtg cgcccatttt acgccatcgg caggctaaag gatattttcg gcgcaaagcc
     4501 gcaatccgct ataatcccca cttttcagac ggcataccat gactgcgctt acccttcctg
     4561 aagacatccg ccaacaagaa ccatccgttc tgctctatac cctcgtttcc gcctacctcg
     4621 aacacaccgc ccaaaccggc gacgaatccc tctcctgcct gtccgacgac cagcacacgc
     4681 tgaccgcatt ctgctacctc gacagccaag tcgaagaagg cggcttcgta cagcttatcg
     4741 cctccggcta cggcgaatac attttccgca acccgcttgc cgacagcctg cgccgctgga
     4801 aaatcaaagc cgtgccgaaa gtcttggaca aagccaaagc cctctacgaa caacacggcg
     4861 aaaccatcga aacgctcgcc gacggaggcg cagacatcga atccctgcgc aaacagttcc
     4921 ccgactttga agaatgggac ggcgcatact acgaagccgc cgaacaagac ctgcccctgc
     4981 ttgcagaaca catcctgtca aaccgggcgg cattcgccca tatcgggcag gcgtgattgc
     5041 gtctgtttcc agcccgtgta aaacagcgta aaatcggcaa acccgaatta tcttccgtcc
     5101 ccatcaagga gcaagctatg ttcttcaagc acatcgaagc cgcccccgcc gatccgattc
     5161 tcggtttggg cgaagcgttc aaagccgaaa cccgccccga aaaagtcaac ctcggcatcg
     5221 gcgtgtacaa agacgcatcc ggcgcgacac ccattgtcaa agccgtcaaa gaggctgaaa
     5281 aacgcctgtt ggaaagcgaa accaccaaaa attacctgac catcgacggc gttgccgact
     5341 acaacgagca aacccaaatc ctgctgttcg gcaaagacca cgaaatcatc gccagccgcc
     5401 gcgccaaaac agcgcaaagc cttggcggta caggcgcatt gcgtattgcg gccgaatttg
     5461 ccaaacgcca gttgaacgcg caaaccatct ggatttccaa tccgacctgg ccaaaccaca
     5521 acgccattgc caaagcggtc ggtatccaag acaaacctta tcgctactat gatgccgcca
     5581 aacactgctt ggattgggac ggcatgattg aagatttgaa ccaagcgcaa aaaggcgaca
     5641 tcgtcctgct gcacggctgc tgccacaacc ctaccggtat cgaccctacg cccgaacaat
     5701 gggaaactct ggcaaaactt tctgccgaaa aaggctggct gccattgttt gactttgcct
     5761 accaaggctt tggcaacggt ttggaagaag atgcctacgg cctgcgcgtg ttcttgaaac
     5821 acaatacaga attgctgatt gccagctctt attccaaaaa cttcggtatg tacaacgagc
     5881 gcgtcggcgc gttcactttg gtggccgaag atgaagaaac agcagcccgc gcccacagcc
     5941 aagtcaaaac catcatccgt accttgtatt ccaacccggc ttcacatggt gcgaacacca
     6001 ttgcactggt gttgaaaaat gatgatttga aagcacaatg gattgccgaa ctcgatgaaa
     6061 tgcgcggccg catcaaagcc atgcgccaaa aatttgtcga gttgctcaag gccaaaggtt
     6121 caacccaaga ctttgatttt attattgaac aaaacggtat gttctctttc agcggcttga
     6181 ctcccgaaca agttgaccgt ttgaaaaacg agtttgccat ttatgccgtc cgttccggcc
     6241 gcatcaacgt cgccggcatt accgacgaca acatcgatta tctgtgtgaa agcatcgtga
     6301 aa
//
LOCUS       DAXFHF010000084         6241 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXFHF010000084 DAXFHF010000000
VERSION     DAXFHF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.084"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            219..1759
                     /locus_tag="ACFIRM_001870"
     rRNA            219..1759
                     /locus_tag="ACFIRM_001870"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1860..1936
                     /locus_tag="ACFIRM_001871"
     tRNA            1860..1936
                     /locus_tag="ACFIRM_001871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1894..1896,aa:Ile,seq:gat)
     gene            1942..2017
                     /locus_tag="ACFIRM_001872"
     tRNA            1942..2017
                     /locus_tag="ACFIRM_001872"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1975..1977,aa:Ala,seq:tgc)
     gene            2364..5255
                     /locus_tag="ACFIRM_001873"
     rRNA            2364..5255
                     /locus_tag="ACFIRM_001873"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5352..5465
                     /gene="rrf"
                     /locus_tag="ACFIRM_001874"
     rRNA            5352..5465
                     /gene="rrf"
                     /locus_tag="ACFIRM_001874"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5501..5620
                     /locus_tag="ACFIRM_001875"
                     /pseudo
     CDS             5501..5620
                     /locus_tag="ACFIRM_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
     gene            5722..6117
                     /locus_tag="ACFIRM_001876"
     CDS             5722..6117
                     /locus_tag="ACFIRM_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC3029438.1"
                     /translation="MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLF
                     SCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPA
                     VLAKLKDQASVGAPAPKGPAKPVLPAVKK"
     gene            complement(6051..6198)
                     /locus_tag="ACFIRM_001877"
                     /pseudo
     CDS             complement(6051..6198)
                     /locus_tag="ACFIRM_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1669 a         1355 c         1869 g         1348 t
ORIGIN      
        1 actgaaaagt atagcacgga atgtcggaaa tatcgagaga tatcttagac aggcggaagg
       61 gatactttat aatttcgcaa cgctctttaa caaaacagat taccgataag tgtgagtgcc
      121 ttgggcctcg cactgtttga aagacagacg agatgatgtt ttgaacattg tcctgtcggt
      181 ttctttgaag cagaccagaa gttaaaaagt tagagattga acataagagt ttgatcctgg
      241 ctcagattga acgctggcgg catgctttac acatgcaagt cggacggcag cacagggaag
      301 cttgcttctc gggtggcgag tggcgaacgg gtgagtaaca tatcggaacg taccgggtag
      361 cgggggataa ctgatcgaaa gatcagctaa taccgcatac gtcttgagag ggaaagcagg
      421 ggaccttcgg gccttgcgct atccgagcgg ccgatatctg attagctggt tggcggggta
      481 aaggcccacc aaggcgacga tcagtagcgg gtctgagagg atgatccgcc acactgggac
      541 tgagacacgg cccagactcc tacgggaggc agcagtgggg aattttggac aatgggcgca
      601 agcctgatcc agccatgccg cgtgtctgaa gaaggccttc gggttgtaaa ggacttttgt
      661 cagggaagaa aaggccgttg ccaatatcgg cggccgatga cggtacctga agaataagca
      721 ccggctaact acgtgccagc agccgcggta atacgtaggg tgcgagcgtt aatcggaatt
      781 actgggcgta aagcgggcgc agacggttac ttaagcagga tgtgaaatcc ccgggctcaa
      841 cccgggaact gcgttctgaa ctgggtgact cgagtgtgtc agagggaggt ggaattccac
      901 gtgtagcagt gaaatgcgta gagatgtgga ggaataccga tggcgaaggc agcctcctgg
      961 gataacactg acgttcatgt ccgaaagcgt gggtagcaaa caggattaga taccctggta
     1021 gtccacgccc taaacgatgt caattagctg ttgggcaact tgattgcttg gtagcgtagc
     1081 taacgcgtga aattgaccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg
     1141 acggggaccc gcacaagcgg tggatgatgt ggattaattc gatgcaacgc gaagaacctt
     1201 acctggtttt gacatgtgcg gaatcctccg gagacggagg agtgccttcg ggagccgtaa
     1261 cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
     1321 cgagcgcaac ccttgtcatt agttgccatc attcggttgg gcactctaat gagactgccg
     1381 gtgacaagcc ggaggaaggt ggggatgacg tcaagtcctc atggccctta tgaccagggc
     1441 ttcacacgtc atacaatggt cggtacagag ggtagccaag ccgcgaggcg gagccaatct
     1501 cacaaaaccg atcgtagtcc ggattgcact ctgcaactcg agtgcatgaa gtcggaatcg
     1561 ctagtaatcg caggtcagca tactgcggtg aatacgttcc cgggtcttgt acacaccgcc
     1621 cgtcacacca tgggagtggg ggataccaga agtaggtagg gtaaccgcaa ggagtccgct
     1681 taccacggta tgcttcatga ctggggtgaa gtcgtaacaa ggtagccgta ggggaacctg
     1741 cggctggatc acctcctttc tagagaaaga aggggcttta ggcattcaca cttatcggta
     1801 aactgaaaag atgcggaaga agcttgagtg aaggcaaggt tcgcttaaga agggaaaccg
     1861 ggtttgtagc tcagctggtt agagcacacg cttgataagc gtggggtcgg aggttcaagt
     1921 cctcccagac ccaccaagaa cgggggcata gctcagttgg tagagcacct gctttgcaag
     1981 cagggggtca tcggttcgat cccgtttgcc tccaccaaaa ctttacaaat gaaagcaagt
     2041 ttgctgtttt tagcagctta ttttgatttg cgaagtagaa taacgacgca tcgatcttta
     2101 acaaattgga aagccgaaat caacaaacaa agacaatgag tttgttttga ttttttattc
     2161 tttgcaaagg ataaaaacct ctcgcaagag aaaagaaaac aaacatagta tttgggtgat
     2221 gattgtatcg acttaatcct gaaacacaaa aggcaggatt aagacacaac aaagcagtaa
     2281 gctttatcaa agtagggatt tcaagtttgc ttacttagtc aacgggtagg taaacgaagt
     2341 caaagaagtt cttgaaatga tagggtcaag tgaataagtg catcaggcgg atgccttggc
     2401 gatgataggc gacgaaggac gtgtaagcct gcgaaaagcg cgggggagct ggcaataaag
     2461 ctatgattcc gcgatgtccg aatggggaaa cccactgcat tctgtgcagt atcctaagtt
     2521 gaatacatag gcttagagaa gcgaacccgg agaactgaac catctaagta cccggaggaa
     2581 aagaaatcaa ccgagattcc gcaagtagtg gcgagcgaac gcggaggagc ctgtacgtaa
     2641 taactgtcga gatagaagaa caagctggga agcttgacca tagcgggtga cagtcccgta
     2701 ttcgaaatct caacagcggt actaagcgta cgaaaagtag ggcgggacac gtgaaatcct
     2761 gtctgaatat ggggggacca tcctccaagg ctaaatactc atcatcgacc gatagtgaac
     2821 cagtaccgtg agggaaaggc gaaaagaacc ccgggagggg agtgaaacag aacctgaaac
     2881 ctgatgcata caaacagtgg gagcgcccta gtggtgtgac tgcgtacctt ttgtataatg
     2941 ggtcaacgac ttacattcag tagcgagctt aaccggatag gggaggcgta gggaaaccga
     3001 gtcttaatag ggcgatgagt tgctgggtgt agacccgaaa ccgagtgatc tatccatggc
     3061 caggttgaag gtgccgtaac aggtactgga ggaccgaacc cacgcatgtt gcaaaatgcg
     3121 gggatgagct gtgggtaggg gtgaaaggct aaacaaactc ggagatagct ggttctcccc
     3181 gaaaactatt taggtagtgc ctcgagcaag acactgatgg gggtaaagca ctgttatggc
     3241 tagggggtta ttgcaactta ccaacccatg gcaaactcag aataccatca agtggttcct
     3301 cgggagacag acagcgggtg ctaacgtccg ttgtcaagag ggaaacaacc cagaccgccg
     3361 gctaaggtcc caaatgatag attaagtggt aaacgaagtg ggaaggcaca gacagccagg
     3421 atgttggctt agaagcagcc atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgt
     3481 cctgcgcgga agatgtaacg gggctcaaat ctataaccga agctgcggat gccggtttac
     3541 cggcatggta ggggagcgtt ctgtaggctg atgaaggtgc attgtaaagt gtgctggagg
     3601 tatcagaagt gcgaatgttg acatgagtag cgataaagcg ggtgaaaagc ccgctcgccg
     3661 aaagcccaag gtttcctacg caacgttcat cggcgtaggg taagtcggcc cctaaggcga
     3721 ggcagaaatg cgtagtcgat gggaaacagg ttaatattcc tgtacttgat tcaaatgcga
     3781 tgtggggacg gagaaggtta ggttggcaag ctgttggaat agcttgttta agccggtagg
     3841 tggaagactt aggcaaatcc gggttttctt aacaccgaga agtgatgacg agtgtctacg
     3901 gacacgaagc aaccgatacc acgcttccag gaaaagccac taagcttcag tttgaatcga
     3961 accgtaccgc aaaccgacac aggtgggcag gatgagaatt ctaaggcgct tgagagaact
     4021 cgggagaagg aactcggcaa attgataccg taacttcggg agaaggtatg ccctctaagg
     4081 ttaaggactt gctccgtaag ccccggaggg tcgcagagaa taggtggctg cgactgttta
     4141 ttaaaaacac agcactctgc caacacgaaa gtggacgtat agggtgtgac gcctgcccgg
     4201 tgccggaagg ttaattgaag atgtgcaagc atcggatcga agccccggta aacggcggcc
     4261 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga
     4321 atggcgtaac gatggccaca ctgtctcctc ccgagactca gcgaagttga agtggttgtg
     4381 aagatgcaat ctacccgctg ctagacggaa agaccccgtg aacctttact gtagctttgc
     4441 attggacttt gaagtcactt gtgtaggata ggtgggaggc ttggaagcag agacgccagt
     4501 ctctgtggag tcgtccttga aataccaccc tggtgtcttt gaggttctaa cccagacccg
     4561 tcatccgggt cggggaccgt gcatggtagg cagtttgact ggggcggtct cctcccaaag
     4621 cgtaacggag gagttcgaag gttacctagg tccggtcgga aatcggactg atagtgcaat
     4681 ggcaaaaggt agcttaactg cgagaccgac aagtcgggca ggtgcgaaag caggacatag
     4741 tgatccggtg gttctgtatg gaagggccat cgctcaacgg ataaaaggta ctccggggat
     4801 aacaggctga ttccgcccaa gagttcatat cgacggcgga gtttggcacc tcgatgtcgg
     4861 ctcatcacat cctggggctg tagtcggtcc caagggtatg gctgttcgcc atttaaagtg
     4921 gtacgtgagc tgggtttaaa acgtcgtgag acagtttggt ccctatctgc agtgggcgtt
     4981 ggaagtttga cgggggctgc tcctagtacg agaggaccgg agtggacgaa cctctggtgt
     5041 accggttgta acgccagttg catagccggg tagctaagtt cggaagagat aagcgctgaa
     5101 agcatctaag cgcgaaactc gcctgaagat gagacttccc ttgcggttta accgcactaa
     5161 agggtcgttc gagaccagga cgttgatagg tggggtgtgg aagcgcggta acgcgtgaag
     5221 ctaacccata ctaattgccc gtgaggcttg actctatcat ttgaaggact tcaagagata
     5281 aaagcttact gactgattca gtcattaccg aatatattga ttaaggcttt accgatttgc
     5341 aacagtttaa gtttggcggc catagcgagt tggtcccacg ccttcccatc ccgaacagga
     5401 ccgtgaaacg actcagcgcc gatgatagtg tggttcttcc atgcgaaagt aggtcactgc
     5461 caaacaccct ttcagaaaac ccccggatat ccgggggttt ttgctttgcc cggaaaaaat
     5521 gttggggatg gcgggacggc atctgtacgg tgtccggtcg ggtttgcgga ggaacggctt
     5581 gaaactttgg gatattcatt ttagaatgac ccgttttata gcggattaac aaaaatcagg
     5641 acaaggcggc gggccgcagg cagtacggat agtacggaac cggttcgccc ggtgcttcat
     5701 caccttaggg aatcgttccc tttgagccgg ggcggggcaa cgccgtaccg gtttttgtta
     5761 atccgccata tcgtcgcaag atgcggtttg ttgtttgcaa cccttaaagg aaaaaccatg
     5821 aaaaaaatgt ttgtattgtt ctgtatgctg ttctcctgcg ccttctccct tgcggcggta
     5881 aacatcaatg cggcttcgca gcaggagctg gaggcgctgc cgggcatagg cccggcgaag
     5941 gcgaaggcca ttgcggaata ccgcgcgcaa aacggcgcgt tcaagtctgt ggacgatttg
     6001 atcaaggtga agggcatcgg tccggcggtg ctggcgaagc tgaaagacca ggcttccgtc
     6061 ggcgcgcccg caccaaaagg ccccgccaaa ccggtgctgc ctgcggttaa aaaatagggg
     6121 aacctgtaaa ggaaggggca tcggctgccg ccggcgcttt tttgttcttt gtttggaagg
     6181 gaaatggctg gagaaagacc tgcgctatat cggggaatga atcgatgcaa aggaaatgcc
     6241 g
//
LOCUS       DAXFHF010000085         6125 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXFHF010000085 DAXFHF010000000
VERSION     DAXFHF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.085"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(211..1598)
                     /locus_tag="ACFIRM_001878"
                     /pseudo
     CDS             complement(211..1598)
                     /locus_tag="ACFIRM_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
     gene            1716..1792
                     /locus_tag="ACFIRM_001879"
     tRNA            1716..1792
                     /locus_tag="ACFIRM_001879"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1750..1752,aa:Arg,seq:acg)
     gene            1810..1884
                     /locus_tag="ACFIRM_001880"
     tRNA            1810..1884
                     /locus_tag="ACFIRM_001880"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1843..1845,aa:Glu,seq:ttc)
     gene            2047..2163
                     /locus_tag="ACFIRM_001881"
                     /pseudo
     CDS             2047..2163
                     /locus_tag="ACFIRM_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2324..3499
                     /locus_tag="ACFIRM_001882"
     CDS             2324..3499
                     /locus_tag="ACFIRM_001882"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC3029439.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(3841..4389)
                     /locus_tag="ACFIRM_001883"
     CDS             complement(3841..4389)
                     /locus_tag="ACFIRM_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC3029440.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(4392..5888)
                     /gene="ccoG"
                     /locus_tag="ACFIRM_001884"
     CDS             complement(4392..5888)
                     /gene="ccoG"
                     /locus_tag="ACFIRM_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC3029441.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
BASE COUNT         1576 a         1658 c         1405 g         1486 t
ORIGIN      
        1 aaaccaccct gcttgtccga tgcctcggga cggacgggca aaccgacacg gcacgcaaac
       61 cgcgtcggca aaaatgccgt ctgaaaaaat tcagacggca tcttcagaca cattacctgc
      121 aaacggcaac acattccaat ccaaaccgat caggcaatca atacgaacgg ctgtttacat
      181 acttaccagc tttcaccaac cggtatcgat ttaaccgatt tccttaatat ttttcctgtc
      241 cgttttaaac ttcgccttaa acacatccgg taaatcttta tcgaaatacc aaagcccgtc
      301 atccatttcc aatgcgccgc ccattccatg cagaacgact tttcccccgt taaaggatcg
      361 gtttcggtaa tttccacctc atgcattttt ccccaatcca aaacaggcaa cttgtgtgca
      421 aacgccaaaa cttgttcgtc agaacggccg cacgccttat cgcatcggcc tgaaacatat
      481 acgggacaag gctcatcctc tgcataaccg tccggaagga taccggctga ggcaggacac
      541 aatccgtatg tttccgccca cgacgacaaa gcccgtctcg ccgccttttt attggcaaat
      601 aatccggtag gcggattatc cgtcacaccg tttttcaaag ccgctgtttt cgcattcaac
      661 atgccgtctg aacctttttc aaacctgacg gtcgtaaatg ttttaagcag atttttggca
      721 gatacataac aacccgaatg ataacgtccg accaattccg ctttaatctt atatgcatgc
      781 aggctgccca atgcgggaaa aaaaaacgga cttcctccgt attgcaccaa tcaaacgggg
      841 gcttttccgg aatccaacaa agccgcaacc tcgccatatg cccgttcaaa cgtaccgata
      901 taatttactt ttcccccgcc gtcgaaacag gcaagcacac ctgtaccgtc gggcaaaccg
      961 tacagctgtt ccctcaaccg ttcgggaagc gcggcaggaa aaagtttcgg attcgtcaaa
     1021 cggaaacact gcctgctcca ctcctcaagc ccgtgtgccg acaggctgta ttccaaataa
     1081 tcacacaacg ccgatacatc cgccatcgca cgatgcctgt cttccacaac aatccccaac
     1141 ctttcgatga taccgtccag gctgtgcttg taaaactgcg gatacagccg acgcgaaagc
     1201 tgcacactgc acaaagcagg cgatgaaaat ccgatacccg cacgatgaaa ctcatgcttt
     1261 aaaaacgtat agtcgaaacg gctgttgtgc gccaccagca cgcacccctt caataccgaa
     1321 aacaacccgc cggcaatttc agcaaacacc ggcgcgtcgg caaccatgcc gtctgaaatc
     1381 cccgtcagtt ccgccacaaa cttcggaatc ggtttttgag gattgaccag ccattcatac
     1441 ctctccgccc ttccctgccc aaacttgacc aaagccactt cggttaccct gtcttcatac
     1501 agattgccgc ccgtcgattc caaatcaacc acggcaacag gcattccaaa ccgtaaaaac
     1561 gccttttcca gcaagggcca gcgggaaaca acaatcattt tattctcttt aaattcaaac
     1621 aacaaaccga tattttacac ttttaaggca tttcatccaa caaaataatt gacagaatcc
     1681 gatgattacc ataaaattca aaaatttatt gcagcgcacc cgtagctcag ttggatagag
     1741 tatctggcta cgaaccagag ggtcgggcgt tcgaatcgct ccgggtgcgc cagtaagaaa
     1801 atacaatacg cgcccatcgt ctagcggtta ggacatcgcc ctttcacggc ggtaaccggg
     1861 gttcgattcc ccgtgggcgt gccagattcc aaatccccga aataatccct cggggatttt
     1921 tattgcctcg gcaattcgtt accatatctt tacctaccca cttcatcaga atctcagacg
     1981 taatcgaatc atattcaaac ttttgccatg caaaccgata tcccgtaacc ggatgcggtg
     2041 tccgttcaat atagtggatt aaatttaaat caggacaatg cgacgaagcc ccagacagta
     2101 caaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc actatatttt
     2161 gtccgattat tggtacagat ataacagcat attccaaagc tccttctgct tttctgttta
     2221 ccactgcaat atttagagta taataccaaa tttgagtaat ggttctaaaa caattaaaac
     2281 catttttcat gagcctgact gattcgtata ttcggagaaa ctgatgcaga atatttttga
     2341 ccctttgatt attcgtggaa aatcccttat tcccatcgtg caaggcggta tgggggtcgg
     2401 tgtttccgca tcgggtttat ccagcgcggt ggcgcgtgaa aacggcatcg gaacgattgc
     2461 cagtgtagat ttgcgccacc ttcacgaaga cctgctcgcc gaatcccaaa tcaatccgag
     2521 tgaagagaaa tatacgtctt tgaactgtac cgcattagac agggaaatcc aaaaagccaa
     2581 aagtgcttca gagggaaaag gactgattgc ggtcaacgtg atgaaggcgg tcaaagacca
     2641 cgccgcgtat gtccgccagg cttgcgaatc aggggcggat gcggtcgtga tgggtgccgg
     2701 cctgccttta gacctgccgg aaatgaccga gggctatcat aaggatgtcg cgctgctgcc
     2761 gattctgtcc gaatcgcgcg gtattaatat cgtcttgaaa cgttggatga aaaaaggcat
     2821 attgcccgat gcgattgtag tcgaacatcc tgcccatgcg gccggacatt tgggtgcatc
     2881 aaccgttgaa ggcgtaaacg atgccaaatt cgacttcaaa cgcgtgattg aggaaacgtt
     2941 tgaagtgttt aaaaatttgg ggctggaggg agaaaaaatc ccgcttattc ttgcgggagg
     3001 catggcaaat tttgaaaaag tcaaaaccgc cctaaagaac tggggagcct ccgccgttca
     3061 aatcggtacg gcttttgccg ttaccgaaga aggagatgca cacctcaact ttaaaaaaac
     3121 gctcgccagt gcggaaactg aaaaagtagt cgaatttatg tctgttgccg gtttgccggc
     3181 gcgcggtatc cgcaccaaat tcctagacag ctatatcagg cgcgaaggca aacttcaggc
     3241 aaacgccaaa gccgacccgc gccgctgtac ccaaggttta aactgcctga ccagctgcgg
     3301 tctgcgcgac gggctttcca aagcagggca attctgtatt gatatccagc ttgctgccgc
     3361 attccgtgga gaagttgata aggggttgtt tttcagggga aaagaccagc ttcccttcgg
     3421 taatgccatc cgcaccgtcc gcgaaacgat acaatatctg ctgacgggga gcgaacctgt
     3481 tgcaacgctc ggacgctgac atcaggtagg atttgatttg caaacaaaat gccgtccgaa
     3541 gggctttcag acggcgtttt tcaggttgtg ttccggaaca gtgtcgaaaa aacggagaga
     3601 aataaaaacg tttttatgtc aacaaaatgc cttccgcatt atgtcggaca tacaacacaa
     3661 atgccgtctg aagcctttca gacggcattt ttcaggctgc ctttcggaat gatgttaaaa
     3721 aataaacggg atgagataca tttattttcg tccgacaaat cagaccatca cgccgaataa
     3781 tcaaacaatg cctgcccgac attgcatata acaaaccaat gcaatgaaaa cacggctttt
     3841 ttatttgctt cctgcattat tgaaaagttt gtccatcggg gtcaaatcga ccgcattgcc
     3901 ctggctggta atccatttgt tctcgacgcg ccacacgcct gccgcgtcct ccacgcgcac
     3961 ataccagtgg ttggccggcg gaagggtttt gaacaccgcc tcatattccg ccctgccgtt
     4021 ctgcgcgctg ccgacgggct tgagggcgac ggtttgatcg tccgccttgc gggtcgggtg
     4081 catcagcagc aggttcaaag gctgtttgcc gtcaaactcg ccgccgacaa acacttttgc
     4141 cgcattcata tcgggagaaa tgaggacctg caccccgata tgccgtctga cggcttcttc
     4201 atcccgatga agctggatgt cgatatgctt gccatcctta taataatcgt ccgtaaccaa
     4261 atctgtcgcg tgctgctgcg cgacaaaaaa catagcgacg ctggcgatga cgacaaaaat
     4321 cggccccgcc atcaagaccc acggccagac gtgtttgtac caaggcttga ttggattgtt
     4381 ttgagacacg gttattctcc gataaaggtt gcgtcttctt ccaaaacaac cggcttgccg
     4441 tctgatgcac cgctttcccg gtattggaag ataaactcga taggatgact gcctttgtcc
     4501 gcgtattccg gaatggtgga cacttggacg ggaagggtta ttgtttcgcg cggagccact
     4561 tttataccgc cttcaggcag cccagtcagg gcgatttcgt caaatccttt gacacttgcg
     4621 gtaatcagct gttctttttc gctgttgttg atgatacgca ggctatatgc gttttccagc
     4681 caacctttgg cgttttcgcg caccattacg ccacggtctt tcagaatatc gacttcgacc
     4741 attttgcgcg tggacaaacc gaccaggaag gcggcgacaa ccactgccaa caccgcgccg
     4801 taacctgcca cgcgcggtct gagcagccgt tttttaatgt ctttttcggc atattcgtgt
     4861 tccagcgcgc tttcggtcgt atagcgtatc aaaccgctcg gataacccat tttgtccata
     4921 atctcatcgc acgcgtcgat acaggcggcg cagccgatac attggtattg caggccgttg
     4981 cggatgtcga tgccgacggg gcagacttgg acgcacatcg cacagttgat gcagtcgccc
     5041 aaacccgcct cttccttgtt gaccgttttc ttgcgggcgc cgcgcggttc gccgcgctcc
     5101 gcgtcataag aaacaatcag cgtgtcctta tcgaacatcg cgctttggaa acgtgcgtac
     5161 ggacacatat gcaggcacac tttttcgcgc ataatatggg cgaagaagaa ggtcataaag
     5221 ccgtaaaacg ctgcggcaaa catcgcgccg ccgcctgctg ctccggtgaa taaatcggga
     5281 acaaactggc ggatggggac aaaccagccg gcaaacgtga tgcccgtcca cgcgcagaca
     5341 aggaaaatca acaggtattt ggtggctttg atgcggattt tggtgaaatt ccacggcgat
     5401 ttttccagct tcaggcgttt gtttctatcg ccttcgacca ggttgtcgat ccacagcata
     5461 atttcggtgt aaaccgtttg cgggcaggaa tagccgcacc acaaacggcc ggcaatcgtc
     5521 gtccaccaaa acagcccgaa ggcgcaaatc atcagcagca gggcaaggta aatcaaatcg
     5581 cccaccccca acgacaatcc gaaaatgaag aaatgccgtt cggggatatt gaaaacgacg
     5641 gcctgccttc cgctccaatt gaaccacgga atgacgtaaa acacaaactg ggtcgccaat
     5701 acggcggcga tacgcagttt ggcgaaccgt ccttccgcct ttttgggatg gatgcgttcg
     5761 ccttcgggat ggatttgaat cacgctggca cgcggatcga acgttttttt cactttcggc
     5821 gcggcttttg tttgttcgga cgtgccgatt ccggatgccg gactgccggc ttggttttcc
     5881 gtggtcattc tgcattcctt agatttttga ttgatggttt gcccgttacc gccgccgttt
     5941 tgcttttcag acgtcatttt tcttgttttt ttaaggcatt gtgtttcaag ttttgagaaa
     6001 atccgctttt cccaaaatat attcccgcta ttgtacaact ttatgcgccg tccggatgta
     6061 ttgggcgaat acatttccca tccgcatcaa aacgccctga ttttaccgca ccgcccgaac
     6121 aaaat
//
LOCUS       DAXFHF010000086         6002 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXFHF010000086 DAXFHF010000000
VERSION     DAXFHF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.086"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..337
                     /locus_tag="ACFIRM_001885"
     CDS             <1..337
                     /locus_tag="ACFIRM_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029442.1"
                     /translation="GGGSDSSMSVQPSVSEQLKDNANVDAKDEKVIEYLKKSSLKDVP
                     KELQAKVLKVKGDEYTGVRKQYAGKLGKGESVKAMLFLDGEEPFSKEQLQKMDVYVNG
                     KKYEGSKGG"
     gene            297..989
                     /locus_tag="ACFIRM_001886"
     CDS             297..989
                     /locus_tag="ACFIRM_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFC3029443.1"
                     /translation="MLMVKNTKVVRGGELDVLPKGLSEQKIEFYGADKEQNYALLKTW
                     VYEQPYSVVRGYFGYSRKDGNPIEGDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSF
                     QYEGRAFGGNGVLSKESLDNHNGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIER
                     IPYRESGSSLGLKDRVSYFGVNEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHK
                     HQAVIGFGGEKK"
     gene            1229..1471
                     /locus_tag="ACFIRM_001887"
     CDS             1229..1471
                     /locus_tag="ACFIRM_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029444.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            1668..2528
                     /locus_tag="ACFIRM_001888"
                     /pseudo
     CDS             1668..2528
                     /locus_tag="ACFIRM_001888"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            2707..3078
                     /gene="rpsL"
                     /locus_tag="ACFIRM_001889"
     CDS             2707..3078
                     /gene="rpsL"
                     /locus_tag="ACFIRM_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC3029445.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            3196..3666
                     /gene="rpsG"
                     /locus_tag="ACFIRM_001890"
     CDS             3196..3666
                     /gene="rpsG"
                     /locus_tag="ACFIRM_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC3029446.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            3685..5790
                     /gene="fusA"
                     /locus_tag="ACFIRM_001891"
     CDS             3685..5790
                     /gene="fusA"
                     /locus_tag="ACFIRM_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC3029447.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            5878..>6002
                     /locus_tag="ACFIRM_001892"
     CDS             5878..>6002
                     /locus_tag="ACFIRM_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3029448.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGG"
BASE COUNT         1695 a         1285 c         1545 g         1477 t
ORIGIN      
        1 gggtggcggt tccgattcat ctatgtcggt tcaaccaagc gtttcggaac aactgaaaga
       61 caatgcaaat gttgatgcga aagatgaaaa agttattgaa tatctgaaga aatcatcatt
      121 aaaagatgta ccaaaagaat tgcaagccaa agtattaaag gttaaaggtg atgaatatac
      181 aggagtgcga aaacaatatg caggtaagct cggtaagggt gaatctgtca aggcaatgct
      241 atttctagac ggcgaagagc cgtttagtaa ggaacaactt cagaagatgg atgtatatgt
      301 taatggtaaa aaatacgaag gtagtaaggg ggggtgaact agatgttctc cccaaaggtt
      361 tgagtgagca aaagattgag ttttacggtg cggataaaga acagaattat gcgctattaa
      421 aaacttgggt ttatgagcaa ccgtattctg tggttagagg ctattttggc tacagtcgaa
      481 aagacggtaa tcctatagaa ggggatggac aaaatcctga agagattcct tttgacttgt
      541 atctgggcga cattagaggc gtggcaacag atgaagacaa attacccaag gctgggagct
      601 ttcaatatga aggtcgggca tttggcggta atggggtttt atccaaagaa tcgttagata
      661 accataatgg cgtatttaga tacacaattg attttgatcg tagaaaaggc tctggctcaa
      721 ttgaaggaat ggaacaatat ggcaagatca aattagaaga agctgctatt gagagaatcc
      781 cttatcgaga gtccggctcc agcttggggt tgaaagaccg ggtttcttat ttcggcgtga
      841 acgaaggggt ggctatgtta gagaaggata atgagattaa aaagtatcat ttgggtattt
      901 tcggcgaagc ggctaatgaa gtagcaggtg ctgtaagcca agagcataaa catcaagctg
      961 ttatcggttt cggcggcgag aagaaataga gaaagcacta tttctattta gtgccgtttg
     1021 tccgggcaag atgccgtctg aagtttcaga cggcattttc ttcaaaaaac agtccgccaa
     1081 atcgaatact gccaagcggc aatgcaatac ctcaaaacag caataatttc ttttaaaaca
     1141 gttgattctt actgaacata aatggccggg caatacgaaa agaaaaatgg cgttgctggg
     1201 cattttattt gaatttgtta tgaaattgat gaaatctaaa aatatctttt ttatgggata
     1261 cctaatcgct tcggcgacgt ttgccgagga tatcggcgtg cctgtcgaac cgattaacgt
     1321 cggtagtcgg gctgcgatgc cgtctgaagg ggaaagcctc gccctcctgc cgtttgcaga
     1381 ggatgtgccg ccggttcgcg atgcaatgcc gtctgaagtt cctaaaagcg cggcaggcgg
     1441 cgatgttcga ggcggccgat ctataccgtg agttgttgtc ggaaaggtcc gaccttgttt
     1501 atccccgttt tgatttgggc gtgatgctgt ttgaagacaa acaatatcgt gaggcattgg
     1561 ttcagttgca ccgggccgag gaggcgttgc cgcccgatat gcggcagctt gcacgggaat
     1621 atatccgtca ggcggaagcg gtgcaggcgt ggcatccttc gttcaatatg aattacgaac
     1681 aaacagataa tgtaaacaat gcttcccctt cacgggacat cgtcctcaac ggccggaaat
     1741 ggataaaatc ggaagacagc ctgcccaaac gcgccaacgg gataaggtac gaactgggcg
     1801 ttgatcggat gtttaacatt gcaggcaatc actttgcccg cttaggcatc agcggttccg
     1861 gtgtgcatta ttggaatgcc cgggatttta gcgaacaggc gtttcatgcc gaagtaggct
     1921 accgttatcg gaatagccgt ctagagtggg gattccggcc ctttgtcaaa caaaaccggc
     1981 tgggcaataa ccgttatacg gcaaatacgg gtattgcgtt ggattacagc cgccgtctga
     2041 atgaaaaatg gcgcagcacc caatctttcc agtatggccg caaacaatac catgacgaat
     2101 atttggcaaa acgttacaac agtaaaacga tttcggtgag cggtacgttc agctattacg
     2161 ctatgccggc ttggcagttg tacgggggta ttagcggtat gtttgataat actgtggaga
     2221 aagagcaggc ttcacggcgt tatggtgtaa gtttgggtac ggtgaaaata ttagacggcg
     2281 gtcttggact gaagttgggt gcgggttaca cgaaacggat atttaaagcc cctgcaacat
     2341 taatttacaa ttttactcgg cgtgatgatt aacatcggat gagcgcggct ttatggcata
     2401 aaaaactgtc gtggaaagga tttacacccc aaataaattt ccgttacaac aagaacaaca
     2461 gcaatatgcc cgccttttat tcgcacagcg gcaagggatg gtttgtcagt atggaaaaaa
     2521 cgtattgaca gtattttctc cagccgtccg actgattgtg agggatgtcg gtaaatattt
     2581 atcggcaaac aagaaaatca tcttttttct tgtcgttatg cttgactgtc tgcttgcaat
     2641 aaaaatataa ttccactctt gccgacatgg tgtcggcaag tatttaactc aacaggacga
     2701 gaaaatatgc caactatcaa ccaattggta cgcaaaggcc gtcaaaagcc cgtgtacgta
     2761 aacaaagtgc ccgcactgga agcctgcccg caaaaacgcg gcgtgtgcac ccgtgtatac
     2821 acgactaccc ctaaaaaacc taactctgca ttgcgtaaag tatgtaaagt ccgcctgacc
     2881 aacggttttg aagtcatttc atatatcggc ggtgaaggcc acaacctgca agagcacagc
     2941 gtcgtactga ttcgcggcgg ccgtgtaaaa gacttgccgg gtgtacgtta ccacactgta
     3001 cgcggttctt tggatactgc aggtgttaaa gaccgcaaac aagcccgttc taaatacggt
     3061 gctaagcgtc ctaaataatt accgggactc aaataggcac gtcggccgcc taagctgaac
     3121 aacggccgag taagtgaata ctcaattggg tattcatggg aatagacccg actgaataga
     3181 ttaaaggaaa ttaaaatgcc aagacgtaga gaagtcccca agcgcgacgt actgccagat
     3241 cctaaattcg gtagcgtcga gttgaccaaa ttcatgaacg tattgatgat tgacggtaaa
     3301 aaatccgttg ccgagcgtat cgtttacggt gcgttggaac agattgagaa aaaaaccggc
     3361 aaagcagcaa tcgaagtatt taacgaagcc attgcaaact ccaaacctat cgtggaagtg
     3421 aaaagccgcc gtgtaggtgg tgcaaactac caagttcctg ttgaagttcg tccttcacgc
     3481 cgtctggctt tggcaatgcg ttgggttcgc gacgcggccc gcaaacgtgg tgagaaatcc
     3541 atggatctgc gtttggcagg cgagttgatt gatgcgtccg aaggccgtgg cggtgcgttg
     3601 aaaaaacgtg aagaagtaca ccgtatggct gaagccaaca aagcattctc tcacttccgt
     3661 ttctaatttt gaaaggctaa taaaatggct cgtaagaccc cgatcagcct gtaccgcaac
     3721 atcggtattt ccgcccatat cgatgcgggt aaaaccacga cgacagaacg tattttgttc
     3781 tataccggtt tgacccacaa gctgggcgaa gtgcatgacg gtgcggctac taccgactac
     3841 atggaacaag agcaagagcg cggtattacc attacctccg ctgccgttac ttcctactgg
     3901 tccggtatgg cgaaacaatt ccccgagcac cgcttcaaca tcatcgacac cccggggcac
     3961 gttgacttta ccgtagaggt agagcgttct atgcgtgtat tggacggcgc ggtaatggtt
     4021 tactgtgcgg tgggcggtgt tcaaccgcaa tctgaaaccg tatggcggca agccaacaaa
     4081 taccaagttc cgcgcttggc gtttgtcaat aaaatggacc gccaaggtgc caacttcttc
     4141 cgcgttgtcg agcaaatgaa aacccgtttg cgcgcaaacc ccgtacctat cgtcattccg
     4201 gtaggcgcgg aagacagttt caccggtgtt gtcgatttgc tgaaaatgaa atctatcatc
     4261 tggaatgaag ccgataaagg tacaaccttt acctatggcg atattcctgc cgaattggtc
     4321 gaaactgccg aagaatggcg tcaaaatatg attgaagccg cagccgaagc cagcgaagaa
     4381 ctgatggaca aatacttggg cggtgaagat ctggccgaag aagaaatcgt aggcgcgttg
     4441 cgtcaacgta ctttggcagg cgaaattcag cctatgctgt gcggttctgc atttaaaaac
     4501 aaaggtgttc aacgtatgtt ggacgcagtt gtagaattgc tgccagctcc taccgatatt
     4561 cctccggttc aaggtgttaa tcctaacact gaagaagccg acagccgtca agccagcgat
     4621 gaagagaaat tctctgcatt ggcattcaaa atgttgaacg acaaatacgt cggtcagctg
     4681 acctttatcc gcgtttactc aggcgtagta aaatccggcg ataccgtact gaattctgta
     4741 aaaggcactc gcgaacgtat cggtcgtttg gtgcaaatga ctgccgcaga ccgtactgaa
     4801 atcgaagaag tacgcgctgg cgacatcgca gccgctatcg gtctgaaaga cgttactacc
     4861 ggtgaaacct tgtgtgcgga aagcgcgccg attatcttgg aacgtatgga attccccgag
     4921 ccggtaatcc atattgccgt tgagccgaaa accaaagccg accaagagaa aatgggtatc
     4981 gccctgaacc gcttggctaa agaagaccct tctttccgcg ttcgtacaga cgaagaatcc
     5041 ggtcaaacca ttatttccgg tatgggtgag ctgcacttgg aaattattgt tgaccgtatg
     5101 aaacgcgaat tcggtgtgga agcaaatatc ggtgcacctc aagtggctta ccgtgaaact
     5161 atccgcaaag ccgttaaagc tgaatacaaa catgcaaaac aatccggtgg taaaggtcaa
     5221 tacggtcacg ttgtgattga aatggaacct atggaaccgg gtggtgaagg ttacgagttt
     5281 atcgatgaaa ttaaaggtgg tgtgattcct cgcgaattta ttccgtctgt cgataaaggt
     5341 atccgcgata cgttgcctaa cggtatcgtt gccggctatc ctgtagttga cgtacgtatc
     5401 cgtctggtat tcggttctta ccatgatgtc gactcttccc aattggcatt tgaattggct
     5461 gcttctcaag cgtttaaaga aggtatgcgt caagcatctc ctgccctgct tgagccgatt
     5521 atggcagttg aagtggaaac tccggaagaa tacatgggcg acgtaatggg cgacttgaac
     5581 cgccgtcgcg gtgttgtatt gggtatggat gatgacggta tcggcggtaa aaaagtccgt
     5641 gccgaagtac ctctggcaga aatgttcggt tactcgaccg acctgcgttc tgcaacccaa
     5701 ggccgcgcta cttactctat ggagttcaag aaatattctg aagctcctgc ccacatagct
     5761 gctgctgtaa ctgaagcccg taaaggctaa tcaggcaaat aggccgtctg aaaggctgaa
     5821 atgatttttc agatggcatt gttctttaat cgatctttaa tgtaaaggaa ttagctcatg
     5881 gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg ttggcaccat cggtcacgtt
     5941 gaccatggta aaaccaccct gactgccgct ttgactacta ttttagctaa aaaattcggc
     6001 gg
//
LOCUS       DAXFHF010000087         5963 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXFHF010000087 DAXFHF010000000
VERSION     DAXFHF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.087"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(125..2872)
                     /gene="tbpA"
                     /locus_tag="ACFIRM_001893"
     CDS             complement(125..2872)
                     /gene="tbpA"
                     /locus_tag="ACFIRM_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFC3029449.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVALTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDVSTYAYFIVEEECKNEGYEKCKAKK
                     DVAGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQQ
                     TFDTRDMTVPAFLTKSDFDANQKQAGSLSGNGKYAGGHKYGGLFTSGENNAPVGAEYG
                     TGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSD
                     KYCRPSADKPFSYYKSDRVIYGESHKLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSNL
                     RHQDYYYQSANRAYSLKTPPQNNGKKINPDPNGSEKNPYWVSIGGGNVVTGQICLFGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGGKIKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFDNAYRDLIVRGYEAEIKDGEEQAKGAPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTV
                     KKHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(2959..5028)
                     /locus_tag="ACFIRM_001894"
     CDS             complement(2959..5028)
                     /locus_tag="ACFIRM_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3029450.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QNVPSKKPEARKDQGGYGFAMRFKRRNWYPQANPKSKEDKIPLSENDWEQTDNGNIKE
                     PSKQKNIIDALPGNDGETLQDSSQEESTSKVKGHHDFKYVWSGFFYKRIQIKREIIDE
                     KITVRSGPDGYIFYKGKDPSRKLPVSGEVMYKGTWDFLTDVKANQKFTDLGNTSAKPG
                     DRYSAFSGELDYIVKTEEDKKEKHKGLGLTTEITVDFEKKTLNGKLIKNNMVINDKGE
                     YTTQYYSLEAQVTGNRFSGKATATEKGESKQHPFVSDSSSLSGGFFGLKGEELGFRFL
                     SDDKKVAVVGSAKTKDKNANGNTAAAGTAGAAGMSSEDTKLTTVLDAVELKLDGKKVE
                     NLDNFSNAAQLVVDGIMIPLLPKDSEGGGSHTDKGENGKTAFTYETTYTPESDKKDTE
                     VQTGAGGMQTVSNTAGGTSGKTKTHYKVQVCCSNLNYLKYGLLTRETADNTMGSGSGS
                     QAAARTAQSAQSMFLQGERTDEKEIPKEQKVVYLGTWYGHIAANGTSWTREASNQKNG
                     NRAKFDVNFKDKKITGTLTAENRSEATFTIDAMIDGNGFKGTAKTGNDGFAPDQNSST
                     GTYKVRIANAEVQGGFYGSAAEEMGGWFAYSGNGQAKNAQTSSGNGNSAGSATVVFGA
                     KRQQLVK"
BASE COUNT         1225 a         1557 c         1487 g         1694 t
ORIGIN      
        1 cacgcctgcg cgggaatgac gggtttcggg attacggtat tgtcgggagg gatggggaac
       61 ggtgaaattt gtgtaaaaaa tgccgtctga agcctttcag acggcatttg cggcgagggg
      121 gcatttagaa cttcatttcc aagctaaatg tgtagttgcg gccgggggcg gcatatcggt
      181 tgtaaacgcc gacatttttg tgttggttga ctgcgccggc ggcagtttgc cgcacatttt
      241 cccaagtaac atagcggtgg ttgaggaggt tgtacacgcc cgcacggagg gtgaagtgtt
      301 ttttaaccgt gtaataaccg gacacatcca caatatacca agggcgggta cggcgcgcgg
      361 tggcttttgt attgcggctg ttgccgttga gcaaagcccg gctgcccaac aactctgtga
      421 tttccttggc tttggaataa gtcagcatac cgttcacacc ccatttgcct tccggttggt
      481 catagcccga gccgacgaca tagcgcgagg gttggatggc atcaaacagg tgtgattgga
      541 tatcggtgcg gtctgcgcgt tttttgatgt cgcggacacg gacacgatta taggcaaatg
      601 tagaatacca accttcgggc aatttatccc atacgccgtt ccaatcgatt ttgcccaaaa
      661 tattgatgcc ggtaatccgc gcgctttggg cattgaggta agccggggcg cctttggctt
      721 gttcttcgcc gtctttaatt tccgcttcat aaccccggac aatcaaatcg cggtaggcat
      781 tgtcgaacca acttgcctcc aagttgccga aatcgccttt aaacacgatg ccggcttctt
      841 tgttgaacga tttttccgga tcgattttga cggcttttat tttaccgccc gaccgccagc
      901 cgtacatttc cgcaaacgag ggcaggcgga agccggttga agtgcggtaa gtcaaatcca
      961 gccagtcggc aggtttgagg acgatgccgg cgttccagga cagggtgcgg tgcgtgccgg
     1021 tggaaacgct gccgtcgtcc gaatgcgtgc tgcggtagtc gtagcgcaag cccgcgccga
     1081 catccgccca cctgcccaaa cggacgttgt cccggaccgc cgcgtaatag cttttgccgt
     1141 tgatgctgcg cggcgtgcag tccgtataag tattgttgcc aaagaggcag atttgccccg
     1201 taaccacatt tcccccgcct atgctgaccc aatagggatt cttttcgctg ccgttggggt
     1261 cggggttgat ttttttgccg ttgttttgag ggggcgtttt caacgaatag gcgcggttgg
     1321 cactttgata ataataatcc tgatggcgga gattagagcc gaagcggtcg taaccgagat
     1381 tcacgctcag gttgtggcgg attttggcgg tatcgaagga ttttttgaat gccgcctgca
     1441 agagcttatg gctttccccg taaatcacgc ggtcggattt gtagtaggaa aacggcttgt
     1501 cggcactcgg gcggcaatat ttgtccgaac cgtcggcgga acagtgcgtc tgctgaaaat
     1561 ggttgtccaa accgatgccc tgccggtcgt aagagaggcg ggcataatcc gcccaagtgt
     1621 ccttatcggc attggtatag acatattcca aaccgtagcg gcttttggtg tgcgtctcgt
     1681 cgtaaaacac gcccgtaccg tattccgcgc ccaccggcgc attgttttcg ccgctggtaa
     1741 acagcccgcc gtatttgtgg ccgcccgcgt atttgccgtt gccgctcaaa gaacccgcct
     1801 gtttttgatt tgcatcaaaa tccgacttgg tcaggaatgc cggaaccgtc atatcgcgcg
     1861 tgtcgaaagt ttgttgcgtg cgttcgagta tgccgccgat gtagtgccgc ttattctcaa
     1921 aacgaaaacc cgggcggaac agccacgacc ggctttcgta tgaaagcgga tcggcgagga
     1981 agcggttggg gcccgtgtag tctcgggtgg aaaccgtttg acgttcgtct ttgccggcaa
     2041 catctttttt cgctttacac ttttcgtaac cctcgttttt gcattcttct tcaacgatga
     2101 aataggcgta tgtgctcacg tcatcaaccg gcgccagcct gttgaagctt tgaacgccgc
     2161 gtccggcggc ttcgtgggcg cggatttccc ccgcgtgccg cccggtgcgg atcagcaaag
     2221 cctccgcacc gcctatgcgc cccgccagcg cgatggattg ggtaagcccc cggtttttgc
     2281 cggaataggc ggttttactc tgaatgcccc actgcctgcc ttccccgata acatcgtcgg
     2341 cggttttggt ttgaaatgcg accgagcccg ccaatgcgcc gctgccttgt tcgaccgagt
     2401 ttgagccttt gctgatttcg acagccttga cgttttcata ctcgatttca ttgattgcgc
     2461 cgctgctgcc cgccgtcctc gtcccgccca atgccgcctg cgcggtgtag gactgtattt
     2521 gcgccaagcc gtccaccgtc aaggcgacgc ggtttttgtc cataccgcgt atcgagtagc
     2581 ccgagcttgc gccgcgcccc tgttcgacga cggcgatgcc ggggtcgtaa cgcgtcaggt
     2641 cgcggatgtc gagtacctgt tccttgctga gggtgtcggc ggttttgacc aatttgccca
     2701 aaccggttac ttcgttatcg cggcgggttt tctgtttttt ggcttttacc tgtatggtat
     2761 ccaactgttt ttcctgtgct tgtccggctt gcacattttc tgcataagcg ggcagcgcag
     2821 tcattaaaga caggcataaa atattgaatc ggaacaaatg ttgctgttgc atagtgtttc
     2881 cctaatcttc gctttcagac ggaatcggca ggaacgatgc cgtctgaagc cttattctcg
     2941 attgttcggc agccgtgctt atttcacaag ctgttggcgt ttcgcaccga ataccacggt
     3001 cgcgctgcct gctgaatttc cattgccgga tgaagtttgc gcatttttcg cttgcccatt
     3061 gcccgaatag gcaaaccaac cgcccatttc ttcggctgca gacccgtaaa aaccgccctg
     3121 cacttcggca tttgcgatgc gcactttata tgtaccggtg ctactgtttt gatccggcgc
     3181 aaatccgtca ttaccggttt tcgccgtacc tttaaagccg ttgccatcaa tcatggcgtc
     3241 aatggtaaag gttgcctccg acctgttttc agcggttaac gtgccggtaa tttttttgtc
     3301 tttgaaattc acgtcaaact ttgcccgatt accatttttc tgattggagg cttcgcgggt
     3361 ccaacttgtg ccgttggcgg caatatgccc gtaccaagtg cctagataaa cgactttttg
     3421 ttcttttgga atttcttttt catcggtgcg ctcgccttga aggaacatac tttgtgcgct
     3481 ctgtgccgtt cgggcggcgg cttggctgcc gctgccgctt cccatcgtgt tgtcggcagt
     3541 ttcgcgcgtc agcaacccgt atttcagata attgaggttg gaacagcaga cttggacttt
     3601 ataatgggtt tttgttttgc cacttgtgcc gcctgccgta tttgaaacgg tttgcatgcc
     3661 gcccgcgcct gtttggactt cggtgtcttt tttatcactt tccggcgtgt aggttgtttc
     3721 gtaggtaaag gctgttttgc cgttttcacc tttatctgta tgactgcccc cgccttcgga
     3781 atccttgggc aggagcggaa tcataatgcc gtcgacaacc agttgggcgg cgttgctgaa
     3841 gttgtcgaga ttttcgactt tcttaccgtc caatttcagc tcgaccgcat ccaaaaccgt
     3901 ggtcagctta gtgtcttcag acgacatgcc tgccgcacct gcggtgccag ccgccgcagt
     3961 attgccattt gcgtttttgt ctttggtttt cgcgctgccg acaaccgcaa cttttttatc
     4021 gtcgctcaaa aagcggaaac ccaattcctc acccttcagg ccgaaaaagc cgccgctcaa
     4081 agaagacgag tcggaaacaa agggatgttg tttgctttcg cctttttcgg ttgccgttgc
     4141 cttgccgctg aagcggttgc ctgttacttg agcctcaagg ctgtaatatt gggtggtgta
     4201 ttcaccctta tcattgatta ccatgttgtt tttaattaat tttccgttca gggttttttt
     4261 ctcaaaatca accgttattt ccgttgttaa tcccaaacct ttgtgctttt cttttttatc
     4321 ctcctctgtt ttgactatat aatccaactc cccggaaaaa gcactatatc ggtctccggg
     4381 tttcgcagag gtatttccta aatctgtaaa tttctgattt gctttcacat cagttaaaaa
     4441 atcccaagta cctttgtaca taacctcccc tgaaacagga agttttctcg agggatcttt
     4501 gcctttataa aaaatataac cgtcaggtcc acttcggact gttatctttt catcaattat
     4561 ttcacgttta atttgaatac gtttataaaa aaaacccgac caaacgtatt tgaaatcgtg
     4621 atggccctta accttagatg tactctcttc ttgacttgaa tcttgcaatg tttctccatc
     4681 atttccaggt aaggcatcaa taatattttt ttgtttggaa ggttctttga tattaccatt
     4741 atccgtttgt tcccaatcat tttcagagag tggtatctta tcttctttag atttaggatt
     4801 cgcctgcgga taccaattgc gccgcttgaa gcgcatcgcg aaaccgtatc cgccttggtc
     4861 ttttcgggct tccggttttt tggaaggaac attttgatac tttggcgcgg gacgcggggc
     4921 ttcggtatcg acagaatcaa gatcgaagct gccgcccccg cccagacaag cactcaacaa
     4981 aaacacaggc agcaccatag cagcctgatt caccaatgga ttgttcataa caaacccgat
     5041 tcaattaaag aatgataagg attattattt tatttatttt taaaaaattt gcaaatgctt
     5101 ttttatattt ttagtaaata aaccaaaata ccgtcattcc cgcgctgctt ttaaaaccgc
     5161 ctgcaaccgc caaacctgct gcaatccgct ccctcccctg cgcgggggag ggccggggag
     5221 agggcacttt ccaagttgcg gcaacctctc ccaaatccct tagtcttcta aaacacaagc
     5281 cttgcggctt actgccctct ctctagcttt ctcccgcagg gagagaggac ggtgcggctg
     5341 ttggggttaa ggtttctgta aactaatcag cttgtgcagg ctgtttttta tttttcatac
     5401 gacctgaaat caacggtaaa tccaagttgc ttttcaaacc gcctgcaacc gcaaaatctg
     5461 ccgcaatccg ctccctcccc cgtgggggag ggccggggag agggcattct ccgagttgcg
     5521 gcaatctttc ccaaactccc ttagtcttct aaaatacaaa ccttgcggct tgttgccctc
     5581 tctctaactc tctcccgcag ggagagagga cggggtggct gttggggtta aggtttatgc
     5641 aaactaatac ggcttgctgc cctctctcta actctctccc acggggagag aggactatga
     5701 tgcccaccgg cggcttgcta ttaggaatat ctgaatcgtc attcccacga aagtgggaat
     5761 ctagaacgtc aaatctcaag aaaccgtttt atccgataag tttctgttcc gacaggttcg
     5821 gattcccgcc tgcgcgggaa tgacgaatcc atccatacgg aaacctgcac cacgtcattc
     5881 ccacgaacct acattccgtc attcccacga aagtgggaat ccagtttttt gagtttcagt
     5941 cattcccgat aaattgcctt agc
//
LOCUS       DAXFHF010000088         5842 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXFHF010000088 DAXFHF010000000
VERSION     DAXFHF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.088"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(45..260)
                     /locus_tag="ACFIRM_001895"
     CDS             complement(45..260)
                     /locus_tag="ACFIRM_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029451.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(274..621)
                     /locus_tag="ACFIRM_001896"
     CDS             complement(274..621)
                     /locus_tag="ACFIRM_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029452.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(806..2080)
                     /locus_tag="ACFIRM_001897"
     CDS             complement(806..2080)
                     /locus_tag="ACFIRM_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC3029453.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLLLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(2141..2413)
                     /locus_tag="ACFIRM_001898"
     CDS             complement(2141..2413)
                     /locus_tag="ACFIRM_001898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029454.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(2488..2715)
                     /locus_tag="ACFIRM_001899"
     CDS             complement(2488..2715)
                     /locus_tag="ACFIRM_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029455.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(2724..3878)
                     /locus_tag="ACFIRM_001900"
     CDS             complement(2724..3878)
                     /locus_tag="ACFIRM_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3029456.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGALLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            4130..4411
                     /locus_tag="ACFIRM_001901"
     CDS             4130..4411
                     /locus_tag="ACFIRM_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029457.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            4408..5451
                     /gene="argC"
                     /locus_tag="ACFIRM_001902"
     CDS             4408..5451
                     /gene="argC"
                     /locus_tag="ACFIRM_001902"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC3029458.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         1431 a         1526 c         1332 g         1553 t
ORIGIN      
        1 ccgtctgaaa aggtttcaga cggcctgcag ccccatattg gctttcaatg cgaccggttt
       61 tcctcacgtt tcaaatacag cagatacgca agaaaagaca aagcccctac aggtcctgcg
      121 acggtaaccg aaaaaaacct gcctttgtaa aacacaccgt cgaagtttaa aaaatagcaa
      181 aggatgtcgg tcaggatgct gatggcaaga taaatcagca cagcggcttt ccagtgtttc
      241 aggtaatgtg cgaaatccat ttcaaactcc cgatcaacag ttgcacgggt cacaatggca
      301 gtcttctccg cattggcagt tgcgcctgtc gatcattttt ccccattttg caccggcaaa
      361 accgccgata tgcccaccca atgcagaacc gccctcagct cctacccatc cgccgcctgc
      421 gccgccgatg gcaattccgg caggatttcc gcctgttacc aatgcgccgc ccgccattcc
      481 cgtgaatgct ccggcaaacg accctaaagc gccgccgacc ttgcttccga cccattctcc
      541 ggagactctg cccctgtgcc cccaagtacc ttctgtgccg ccgaaaacag cggtcatttc
      601 gttcatggtc aattctctca tttcaaaact ccttttatcg tcaatcgaaa aattgaaagg
      661 gcagataccc tatccttgct tttacagaca gagtatctgt aaaagggggt agtttcaggc
      721 agcttcggtg tttatgtttt tatgtagtgt tttacaggtc gtctgaagag gtttcagacg
      781 actttttatc tttcagcctc aagccttaca acctgcccga catactataa atcggctcca
      841 atacccattc gtacagccgc cgtttctcgt gtaggatgtc tgcttccagc gtcatgccga
      901 tttgcagcgg tttttcctca ccgtatgcag tgatggttgg tttgtcgggt tttattttca
      961 cgagataaac aggttcgttg ctcttcgcca aatcggagga taccatgccc aatcccgaca
     1021 attcctgtct gcccagtgcc gtttttgcca ctgatacgac actgccggaa gcaagcccga
     1081 atttctgata gggatatgcc tgataacgta ggacaacctt gtctttcggc ttgataaagc
     1141 ctgctgcact gctggggata tatagatggg catataactc ggtacgttcg ggaacaatgc
     1201 tcaagagcag tttggaagga tcaacctgcc gtccgacttc gacgttcggt attgctatat
     1261 aacccgaccg tcctgcacgg atgatttgtt cagagcgcat ttcaaaatcc aaaacttctt
     1321 gagaaatatc ggcaatggtg cgttcaagct ggctttgttc tgtctcatgc cgtttgggga
     1381 ggctggccaa tgtcagattc tgcgtgcgga tttcctgaag cagcccggct tcttctcggc
     1441 ggtaggcatc aagtttggct ttctgctcta aaagctctgc ctcgacattc atcatttctt
     1501 gttttgacac tgcatcattg gcggatagga aacgatattt ccgcaacatt tcttccgcaa
     1561 gtctaatgcg ccttttctgg ccgtctatct gttgcaaaat atggagtttc tggttttcca
     1621 aacgttcgac agttgcttta aggctgcgcg tttcattctc gtgtatcagc ttcagacgac
     1681 ccagttcctg ttctgccaac gttttcttca aaaccgcttc cgttttcaac tgctgctgca
     1741 cgctacctcc tgcgccgaaa cgtgaggtcg aaagcgcaaa tagcttgtcg ccagccttga
     1801 ccttttctcc atcttccacg aatttcgctg taattgtccc cgtatccggt gcatacaccc
     1861 tgattacgcc cgatgcaggt aaaatttgtc cctccactgt tgtctttcgc gtatagttac
     1921 cgaatatcaa aaacaggata atcagtaacg cagatatcga tgcaaatgtc gtccataggg
     1981 aaaatgacaa cggtcgtgtc agaatcactt tacccgtcag gctggtttgc cgggcaatgg
     2041 cgacttcagg gcggaagaaa ggttgcttgg gtctattcat aaaattgaag ttaagaaagt
     2101 ttcagacgac ccctagagat cgtctgaaca atgagaaata tcagcagtaa tctgtaccgt
     2161 cagtgtagcc gtttcctgat ttatctgctt ttgttgcggg agcagttaat ccatgttcaa
     2221 tctcaaagat tggtcttccg ttataaggag gtgcattaac ggcatcattt acccaatcac
     2281 gagtcagatt gtatacacca tttgcaccat atagcaccga aaaaattttt cggcaggaaa
     2341 tgaactgtcg ctgcagcagc aggtattgca acaaaatccc cccatgtggg acctcctttg
     2401 gttgtggcag cattagctac atttccagct atattgtctg ttacaggacc tccccctgaa
     2461 accagcttca attcatgaag ttgaagttca atcattttta tactcctttt cttggttggt
     2521 attcctaaaa attcggctaa caaaaacata tggcagatat attgaaaaaa aattcaaagt
     2581 accctgaata aaattcaaat tccaactata tttgttaatg tagtcgagaa gaaacatatc
     2641 tgataaagaa tatagcaatt gataacaagc tattactaat attacgaaaa atataaactg
     2701 cttccagttt ttcatagaat ccctcacaaa atttccagaa aatctaactc tatcaactga
     2761 tagatcaact tcctaacttc ttcatgtttt gcctgattga agttaaccag tagatttttc
     2821 aacaataacg gttcattctt accgatgtgt tctaacactt ttttccccaa ctcatctacg
     2881 cttatcttca tcccattccc aatcaaatat cccttttcca acgtatccaa attattggca
     2941 tttaatctca acctgacgtc gtctgaaagc ggagtagcgt tgggattcgc gaactgttcg
     3001 agatgaaaag cggtatcggt acgttctttg ccgagaaagt cttcactgaa ggcttcgtaa
     3061 ttgacggggt cggcaatcat ggcggcaatt tgtgcggcag tatcgttgat acgcgtccta
     3121 tcttgctccc agtctgagaa actgtggcgc agactttcta tggtgggaaa tttcttcatt
     3181 agccactcga ggtaattata gccgtttggt gggaaggtac cgacagcgaa gtggaaggtt
     3241 tcacagccga gcgggatagg tctgtgccac caaccgcgtg ggatgtagag gacatcacct
     3301 gcttcaagga taatatccat atcgatatgt tcaggaatgg aaatatcagt atctttagtc
     3361 tgttgcatat acaatggcat agggaaatca ggggcagtaa gttgccaacg tttcttgccg
     3421 aaaagctgga cggcatacat atctcgggcg tcccaatggt ttttataaga ttcgtcgctg
     3481 ccaaaagcaa gatatccact aacaatagta tgtgcgccgg caaagcgggc gacttgacgg
     3541 gcgatatggt ctgaaaacgg ctcgttgtta atatggttat agactaacaa cgcaccattc
     3601 ttcatatgtt cgtagataac ggatttaata aaacggtagc gagttttgcc caaatcgtcg
     3661 aaactttcga cgtattcttc tttaggaacg attgcgcctt ttttacgcag atgaaacagc
     3721 ggtgcggttg ggtctgctcg ttggtatatc tcgttgatat atttacaaga tgcggcttcg
     3781 agattccgaa ccgctccttt aaagagcttg ggcttttgat acagataagt ctgtcggaac
     3841 gttttaggac taatgccgaa gtcgagatgg atgcccatta cttcccctta ctcagaaaat
     3901 atttaaaatt tataatgtta catatagtta caaatattag agttttttgt gtgtgcgtca
     3961 aggaattgtt gacaatttta gttaaaaatt tgtctccaaa cggaaaaaag cggtttgttt
     4021 tttgttgtta acatttttat tgtaattaaa taaaaggtcg tctgaaaacg gttttcagac
     4081 gaccttttgc tataatcggg cttcatcgcc ccgttcggtt tggaacctta tgaaaaccct
     4141 cgtcctcctc ctgctttcct tctccacgac caccgctttc gccgcatacg gtttgggttt
     4201 ggggcaggca ccgaaatatc ctgccggctt tcgcgcctac ggttatgttt attccggacg
     4261 gcagggctgg gttttaaaaa cagaggcgga tgccattaaa ttagacacgc ttttcaaacg
     4321 ctttgtgtac cgtccttccg ccgccaatca aaaccccgtc ggacagcgtt cggacggcat
     4381 acccgccaac cacacaaagg aaaaaccatg agcaaaaaaa tcaaagccgg cattgtcggc
     4441 gcgacgggct acacgggcgt ggaactgctg cgcctgcttg ccgcccatcc agatgtcgaa
     4501 gtcgccgccg taaccagccg cagcgaagcg ggaaccgcag ttgccgatta ctttccaagt
     4561 ctgcgcggcg tgtacggcct cgccttccaa acgcccgacg aggcaggttt ggaacaatgc
     4621 gacatcgtct tcttcgccac gcccaacggc atcgccatga aagacgcgcc gcgcctgatt
     4681 gaacagggcg tgcgcgtcat cgacctttcc gccgacttcc gcatacggga cattccgacc
     4741 tgggaacact ggtacggcat gacccacgcc gcccccggcc tcgtttccca agccgtgtac
     4801 ggattgagcg aactcaaccg cgaagccgtc gcacaggcgc gcctcgtcgc caacccgggc
     4861 tgctacccga cctgcgtatc cctgccgctc gtgccgctgt tgcggcaatg ccgtctgaag
     4921 cccggtatgc cgctgattgc cgactgcaaa tccggcgtgt ccggcgcggg caggaaaggc
     4981 aatgtcggtt cgctgttgtg cgaagccggc gacaacttca aagcctacgg cacagccgga
     5041 caccgccacc tgcccgaaat caggcagacc atcgccgggc ttcaggacgg catcgccgaa
     5101 ggattcgtgt tcacgccgca cctcgcgccg atgatacgcg gtatgcacgc caccgtttac
     5161 ctccaccttt cagacggcag cgaccccgaa accgtcctgc gcgactacta ccgcgacagc
     5221 ccgttcatgg acatcctgcc cgccggttcg acacccgaaa cccgcagcgt gcgcggcgca
     5281 aacctctgcc gcatcagcat ccgtcaggcg gcgcaatccg atgtgtgggt cgtcctttcc
     5341 gtcatcgaca acctcgtcaa aggcgcggcg ggtcaggcag tccaaaatat gaacattatg
     5401 ttcggactgg aggaaacaca cggcttgggc ggcatccccc tgctcccctg aagcgcaaac
     5461 ggcaaaccgc aggcatcgtg cctgcggttt tttgatgccg tctgaaagcg gcggtttttt
     5521 tgggttcgga cggcttttgg ctcatcattc agcgaaaaca aaaatctaaa ataccgtcat
     5581 tcccgcaaaa gcgggaatct agtttatccg gcttcagcaa tttccgacac atttccacac
     5641 gcttcgattc cgtcatttct ccggtttcaa tcgtttccga taacaccgtg gtttttcatt
     5701 tctagattcc cgcctgcgcg ggaatgacgg cggggggctt gccgttttcc cgataagcct
     5761 ctgcggattc tcattgccgg atttccgccc gcttgggaat aacgggattt aaaatcatga
     5821 aaatgtgtca aaaataatat ag
//
LOCUS       DAXFHF010000089         5710 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXFHF010000089 DAXFHF010000000
VERSION     DAXFHF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.089"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(363..518)
                     /locus_tag="ACFIRM_001903"
     CDS             complement(363..518)
                     /locus_tag="ACFIRM_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029459.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSFRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(535..2331)
                     /locus_tag="ACFIRM_001904"
     CDS             complement(535..2331)
                     /locus_tag="ACFIRM_001904"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC3029460.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTANEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2789..4042
                     /locus_tag="ACFIRM_001905"
     CDS             2789..4042
                     /locus_tag="ACFIRM_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC3029461.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     ELALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLVAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGRDKTGNAELFAWN
                     IWVAGSGLAVIFWFDGAMAELLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4197..5708
                     /gene="lysS"
                     /locus_tag="ACFIRM_001906"
     CDS             4197..5708
                     /gene="lysS"
                     /locus_tag="ACFIRM_001906"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC3029462.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDGFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1184 a         1505 c         1687 g         1334 t
ORIGIN      
        1 tctccccggc cctcccccac gggggaggga gcagattgcg gcagatttgg cggttgcagg
       61 cggtttgatt tgcgagggaa tcggacgcgc aggcgggaat ccggatttgt ccgcgcggaa
      121 acttatattt cgtcattcca ccaatcccac ccacagccca cttttccgcg ccattcccgc
      181 gcaggcggga atccagtccg ttcggtttcg gttatttccg ataaattcct gctgcttttc
      241 attcctagat tcccactttc gcgggaatga cgggatttca gggtaaagat ggcggcagcc
      301 ggaggggtgt gaagagctgt tttcgcagga atgacgatat gcggatttgc tgttttcatc
      361 cgctaccgat gccgtctgaa gcctgtttgg cgtttcagac ggcattgggt tttttgtgca
      421 tttgccgcaa aacggatcgg tgtctgcttg cggaaagacg ataacggtgc ggaatgtttt
      481 gggaaaccaa tgccgtctga agggctttca gacggcattt ttgtaccgtg ccgtttaacg
      541 cgccagcggt tcggtgcgtt tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc
      601 gaggcggcgg cggacttcgg cgtggtagcg gttgacccag tcgatttcgc cgtcggtcat
      661 cagggcggtg tccatcaggc gtgtatcgat ggggcagagg gtcagggttt caaaacagag
      721 gaagctgccg aactcggttt cctgcgggtc ggcgacggct tggttggcgg cgaggttttc
      781 aatgcggatg ccccatttcc ccggacggta gaggccgggt tcgatggagg taaccatgcc
      841 ttttttcatg gcggtttcgg gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc
      901 gtggacgttg aggaaatagc ctacgccgtg tccggtgccg tggccgtagt cgcattgcgc
      961 ctgccacagg ggtttgcggc aaatcgcatc aatcagcggc gaggggatgt tttcggggaa
     1021 cacggcttcg gcaagcgcga tatgggcttt gagaacgagg gtgttgtcgc gtttttgttc
     1081 ggcggtgggt gtgccgacag ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg
     1141 cgcgccggag tcgatgagca aaagcccgtt gccgctgatg gtgctgtggc tttcgggtgt
     1201 cgcgctgtaa tgcggcagtg cgccgttggc gttgaagcct gcgatggtgt cgaaactcaa
     1261 tgaaatgaag cctgggcgca cgctgcggtg gcgataaagc atcgtatcca cgtcgatttc
     1321 ggtcaggctg ccgcctttgc cgatgatgtc ttcaaactcg gcgaagaagc cgcacaacgc
     1381 cgcgccgtct tgttccatcg cttcgcggat gcgggcgatg tcggcttcgg atttgacgga
     1441 tttgaagaag gtggacgggt tgatgccttc aatcaggcgc gcgctttcgg gcaggcgcac
     1501 gagcgtactg acggcggttt tgttcggctc gatgagcagc gcgccgccga tttgcgcgag
     1561 tttgtcggca acttgggcgt aaggctcgac cgtgatacct gcggtttgca gcgcggcggc
     1621 ggcttcggcg ttcagacggc attgttcggt aaacaggacg gcactgtctt tgccgatcag
     1681 caggaaggac acgaaaacag ggttgaaagg cacgtcgctg ccgcgcaggt tggtcagcca
     1741 agcgatgtcg tcaagcgagg aaaccaagtg gtaatccgcg cctttttccg ccataactgc
     1801 gcgtacgcgg gcgagtttct cggcggcggt ttcggagacg taggcgtggt cgtggatgaa
     1861 cacggtttcg gcggggatag cgggacggct tgtccacact tggtcaagca ggtcgtcggg
     1921 gtgttggatg cggatgtttt tggcggcgag cgattgcgcc aaagtgcgtt tgccggtgag
     1981 cgacaccata tcggaaggga tgccgacggc agcgttttcg ggcaggtttg ccgcgagcca
     2041 ttcgttgtac ggcggcactt gcccgctttt ttgcagctca atgccgctgc cggaaagctg
     2101 tttggcggct tgttcccaat agcggctgtc cacccatacg cccgcttcat tggcggtaac
     2161 gacgaatgtg ccgaccgagc cggtaaagcc tgacaattcg cgccgcgcct gccaatgctc
     2221 gggcaggtat tcggataggt gggggtcggc ggaagggatg acgagtgcgt ccaagccttg
     2281 cgccttcatg gcttcgcgca atgcggacag gtaattcgat acggtattca taacagttcc
     2341 tccaatcggg tttcgcggct tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa
     2401 acataaccat attatcaaat cgccagcctg ctgcaaaacg gacgggctga tcggatggtg
     2461 cgtgtttgca cgaaaaaagc gatgtaaggt tgtttgttgt aaaatttcag gcataaaatg
     2521 tcgtttcatg ccttcttttg gaagtaatac ggaaaatata gtcaggtttg agcatgaagt
     2581 tctaatagtg tgtgttggca ggtatttgga ttacgttaca atacgcgctt tcatcaaaat
     2641 ttccctaccg ggcggtatcc tgtttttgaa gacggtagga tttgggtttg tttggggtca
     2701 atcccgccca aattggagca atagataggg ctgtgtccga atatgcggct ctgtgtgttg
     2761 aaacacagtt aaaaatacgg aagactttat gtccgaacaa catatttcga cttggaaaag
     2821 caagatcaac gcattgggac cggggattat gatggcttcg gcggctgtcg gcggttcgca
     2881 cctgattgcc tcgacgcagg cgggcgcgct ttacggctgg gagctcgcgc tcatcatcat
     2941 cctgaccaac ctcttcaaat acccgttttt ccgcttcagc gcgcattaca ctctggacac
     3001 gggcaagagt ctgattgaag gttatgccga gaaaagctgc gtttatttgt gggttttttt
     3061 gattttgtgt atcgcctccg ccacgattaa cgcgggcgcg gtcgccattg taaccgccgc
     3121 catcgtcaaa atggcgattc cctcgctgat gtttgatgcc ggcacggttg ccgccttgat
     3181 tatggcatcc tgcctgatta ttttggtgag cggacgttac cgcgctttgg atcgcgtttc
     3241 caaaatcatc atcgttactt tgagtatcgc cacgcttgtc gccgccggca tcgctatgtc
     3301 gcgcggtatg cagatgcagc ccgattttat cgagccgaca ccgtggacgc ttgccggttt
     3361 gggcttcctg atcgcgctga tgggttggat gcccgcgccg attgagattt ccgccatcaa
     3421 ttctttgtgg gtaaccgaaa aacaacgcat ccggccttcc ggctaccgcg acggcatttt
     3481 cgatttcaac gtcggttata tcgccagtgc ggttttggct ttggttttcc ttgcactggg
     3541 cgcgtttgtg caatacggca acggcgaagc agtgcagatg gcgggcggca aatatatcgg
     3601 gcaattgatt aatatgtatg ccgtaaccat cggcggctgg tcgcgcccgc tggtggcgtt
     3661 tatcgcgttt gcctgtatgt acggcacgac gattaccgtt gtggacggct acgcccgcgc
     3721 cattgccgag cccgtgcgcc tgctgcgcgg cagggataaa accggcaacg ccgagttgtt
     3781 tgcctggaat atttgggtgg cgggcagcgg tttggcggtg attttctggt tcgacggcgc
     3841 aatggcggaa ctgctcaaat ttgcgatgat tgccgccttt gtgtccgccc ctgtgttcgc
     3901 ctggctcaac taccgcctcg tcaaagggga caaacgccac aggcttaccg ccggtatgaa
     3961 cgcccttgcc attgtcggcc tgctctacct ggccgggttt gccgttttgt tcctgctgaa
     4021 cctgacgggg cttttggcat agtttccaaa acacggatgc cgtctgcaag attttcagac
     4081 ggcatcgccc tttcttggaa aaaaacaccg acatcgcgta aaatacgcgc aaattttgtg
     4141 attcggtcag gtcgtctgaa acagactgcg cctgatttat tttttcggaa aaccctatga
     4201 gcgaacaaaa ccatccgcaa accgagccgc agttggacga aaaccaaatc atcgccctgc
     4261 gccgcgaaaa actgaacaac atccgccaac agcgcaacgc ttaccccaac gatttcaagc
     4321 gcgacagctt tgccgccgat ttgcaggcgc aatacggcga aatcggcaaa gaagaactcg
     4381 acccgcaggc cgttcccgtt aaaattgccg gccgcatgat gctcaaacgt caaatgggca
     4441 aagccagctt cgccaccatt caagacgtta ccggtcaaat ccagctttat ctgaacaaca
     4501 aaggcgtgag ccaagaagtt ttggacggct tcaaccactg ggacttgggc gacatcgtcg
     4561 gtgcggaagg cactttgttc aaaaccaacc acggcgaact aaccgtgcgt gtgtccgaca
     4621 tccgcctgct gtccaaatcc ctgcgtccgc tgcccgacaa acacaaaggc ttgagcgatc
     4681 aggaaaccaa ataccgccaa cgctacgtcg atttgattgc caatgaagaa tcgcgcaata
     4741 cctttatcaa acgcagccaa atcatccaat ctgtgcgtaa ttttatggtg ggcgagcatt
     4801 atctcgaagt cgaaaccccg atgatgcacc cgattcccgg cggcgcgaca gcgaaaccct
     4861 tcgttaccca ccacaatgcc ttggacattc cgctctacct gcgcatcgcg cccgagctgt
     4921 atctgaaacg cttggtggtc ggcggcttgg aacgcgtgtt tgaaatcaac cgcagcttcc
     4981 gtaacgaggg catgtccgtg cgccacaacc ccgaatttac gatgattgaa ttttacgaag
     5041 ccttctccga ctacgaacgt atgatgcaga tggcggaaga catcatccgc aacgcatcgc
     5101 gcacggtaaa cggcacggca aacatcactt acaacggcaa agaagtcgat ttggaaagcc
     5161 cgttcgaacg tctgaccatc ctcgaagcca tcaaaaaata caatccgcac tacaccgacg
     5221 agcagttgaa cgatgcggaa tggctgaaaa aagaaatcgt caaacacggc gaaagcctgc
     5281 cgccgtcccc gggcatcggc agcctgcaac tcgccctgtt tgaaggttgc gccgagggca
     5341 aactgtggaa tccgaccttt attgtcgatt acccggtcga agtttccccg ttggcgcgcg
     5401 cttcggatac gaaacaaggt ctgaccgaac gcttcgagct tttcgttgtc gggcgcgaac
     5461 tggcaaacgg ctattccgag ttgaacgacc ccgaagacca agccgaacgc ttcaaatcgc
     5521 aagtggcgca aaaagatgcg ggcgacgacg aagccatgca ctacgatgcc gactacatcc
     5581 gcgcgatgga attcggtttg ccgccgaccg gcggttgcgg catcggcatc gaccgcttag
     5641 tgatgctgct gaccgatttg caaaccatcc gcgatgtgat tctgtttccg cagatgcgtc
     5701 ccgagtaatc
//
LOCUS       DAXFHF010000090         5557 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXFHF010000090 DAXFHF010000000
VERSION     DAXFHF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.090"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(348..3182)
                     /gene="lbpA"
                     /locus_tag="ACFIRM_001907"
                     /gene_synonym="tbpA"
     CDS             complement(348..3182)
                     /gene="lbpA"
                     /locus_tag="ACFIRM_001907"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC3029463.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENAKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAVSRPEQLSHNAARISEHTG
                     EYTADGKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYF
                     DFSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQ
                     ELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVAD
                     QKTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNR
                     IKPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELA
                     YLAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAE
                     STANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3179..5371)
                     /locus_tag="ACFIRM_001908"
     CDS             complement(3179..5371)
                     /locus_tag="ACFIRM_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3029464.1"
                     /translation="MRKLNYYGIALLPLMLASCGGNFGVQPVVESTPTAYPVTFKSKD
                     IPTSPPPAEPSVETTPVNRPAVGAAMRLLRRNIATSDKDGNDFPNSKQAEEKLSFKED
                     DVLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYNYKFVDAGYVYTKNGKDEI
                     EQNSGGKRFTHRFGYDGFVYYSGERPSQSLPSAGTVKYFGNWQYMTDAKRHRTGKAVA
                     SDDLGYITFYGNDIGATSYAAKDADDREKHPAEYTVDFDKKILKGELIKNQYVQKRND
                     PKEPKKPLIIYNITADLNGNRFTGSAKVNTEVKTRHADKEYLFFHTDADQRLEGGFFG
                     DNGEELAGRFISNDNGVFGVFAGKQKTNASGTNPAMPFGKHTKILDSRKISVDEATDN
                     HARKFAISPMPDFGHPDKLLVEGREIPLVSQEKTIDLADGRKMTVSACCDFLTYVKLG
                     RIKTERPAAKPKAQDEEDSGINNGEESEDEEIAEESEDEVSEDDNGEDEDEVVEEEAD
                     EAEEIEEEAEEEEPEEESPEEGNGVSDGIPPAPEAPKGRDIDLFLKGIRTAEADIPKT
                     GTAHYTGTWEARIGEPIQWDNKADKAAKAEFDVDFGNKSISGTLTEQNGVEPAFRIEN
                     GVIEGNGFHATARTRDNGINLSGNGSTNPQSFKADNLLVTGGFYGPQAAELGGTIFNK
                     DGKSLGITEDIENEVENEADVGEQLEPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT         1201 a         1468 c         1297 g         1591 t
ORIGIN      
        1 ctataatctc aaaaaattta tgccattaaa tcaataattt ctgatgaatt ttgaaaactt
       61 aatcccgtca ttcccgcgca ggcgggaacc cggttcattg agcttcagct atttagaata
      121 aattttgaaa ctctaatcgc gtcattccca cgaaagtggg aatccggttt tttgagtttc
      181 agtcatttcc gataaattgc cttagcattg aatgtccgga ttcccgcctg cgcgggaatg
      241 acggcggaaa tcttgtttat attgaatcaa aaaaacctgc accttaatca gttggcggtt
      301 tagtccgact tttggggtgc agatcaagct tccagacggc atttccttta aaacttcatt
      361 tcgagcgcga ggctgaagtt cctgcccggt gcggcatacc ttccatagtt gctgtcgccg
      421 ccgtgccggt ttgccgtgct ttccgcagtc tggcgcaagg attcccaagt aacgtagcgg
      481 tagttgccga tattgtagat agccgccctc aaggtcagcc gttttttcag attcagataa
      541 gcggaaacat ctgccgtttt ccaagaagac gtaacccttc ctgccgaata tcgtttttga
      601 tcgcctgcca gataagcaag ctcgtcaggg tttttccctt tggaataggt cagcataatg
      661 tttgcgcccc atttcccctc gggctggtcg tatccgaacc ccaaaacata acgcgacggc
      721 tgtaccgcat ccaaagcata gctgcggagg gacaagtccg gccggttgga taccgatttc
      781 ggtttgatac ggttgtacgc caatgtggtg tacaggcctt cgggcagttt gccgtatacg
      841 ccgttccagt cgattttccc caagatgttg atgccttgaa gcgacatatt ttgggcattg
      901 taataatcgc gtatatcaat ctctgtcaat cgtcctgctg aatccggcaa tttggttttt
      961 tgatcggcaa cggcaatcat atcggtataa cggttgcgga aactgctgat ttccaaaaag
     1021 ccgaaatcgc ccttccactg caaaccgatt tcccggttgg ctgccttttc cgatttcagg
     1081 gcgggacgct gccagccttt cggataatcg tgataaatgt ctatcccgaa aagttcttgg
     1141 aaggaaggcg ttctgaagcc gctggaggcg cggtaagaca cggaaaaatg ccggttcggt
     1201 ttgaacacga tgccgctgtt ccacgaacgg tcggcatacc gcccgctgcg gacgagttct
     1261 tccgacgtgg tgaagttttt ccggtcgtac ctgccgccca agctgaaatc aaaatatttg
     1321 ccgattgaaa aacggtcgtt caaagaaatg tggatattgc tgccgttgat ttttcttggc
     1381 acgcatttgc gggaacgcag ggtttcgatg tagccgcaga ccgacccttc gacgacttcg
     1441 ggcttaccca aacggtactt atctttaccg tcggcggtat actcccccgt gtgttccgaa
     1501 atccttgccg cattgtggga aagctgttcg ggacgggaga ctgctttgga agcatcgtaa
     1561 ccgaagccca aagtcagatg gtgtttcgtc catttgtttt tcagcgactt ctcaaacgag
     1621 gcattcaaaa cattgtgctg ttcgcggtag tggaaacggt cgctgctgtc gtaggaatac
     1681 ggtttgtccg ccgacgcgcg gcaggatttg tccacggcag gatacacggc gcaattcagc
     1741 ttcagcgtgt tgttgtcggt tgccacgccc tgtttgtcaa acgacaacac cgccttatcc
     1801 gcccagttgt ccgagtattt cgcattttca taacggtaca gcaaacccat acggcggcgg
     1861 cggtgatgtt cgtcgataaa tttggtgcgg gaatatttca aacctatgcc cttgaccaaa
     1921 tttttatcgc ctttccactc ttctatattc ggcacaaaat acaagccgtc gcggaaatcg
     1981 tcgccgtcgt acaccccgct cttgtctcta aacttttccg cctcgtccgt accgtaatac
     2041 tgtttttccg tcatatcgcg gatgtcgtaa cgctgtttgg tatcctcaaa cacgccgccg
     2101 acataatgcc tgccgccgaa gcggtagccc agcttggcaa gccaagagcc gctgcggtaa
     2161 tccatcggat cgggcaatat cctgccgccg cccgtgtagg cttgggcgga caggttttcg
     2221 tggcgcgcct gcgcctcccg cacctgcgcc tcttcttcag cacttaaagg ctgattttgt
     2281 tcaatacgtt cttttaccca gcggttgagc tggcggttca aatatatccc gtagcccgcc
     2341 aattttgcca cgggcttgag tgtattctcg ccctctacta agaaaaatgg ctctgttgtc
     2401 ttgcgtttaa tatcgtatgt ctggcggaac gcgtccaaac ggtctatgcc gtattccacc
     2461 ccgtccgcaa tatcgccgtg cgggcgcgtt tcccgccctt ggcgttcggt tcggattagc
     2521 agcccttccc aaccgtcttt gctgaacccc gcgccgagcg acttcataaa ttggcggttt
     2581 ttactgccgt aagcggtttt tgcctgtatc ccccagcttt tgccgtctga aatcaggtct
     2641 gccgcctctt tggtgcggaa ggcgaccgcg ccgccgagtg cgccgctgcc gtgatcggac
     2701 gaaccggcgc ctttgtcgat ttccaccgtg ctgatgtttt catattcgat ttcgttgatt
     2761 gcgccgctgc cgccgcgtcc gccgtatccg ctcaacgatc cctgcacggt aaacgcctgt
     2821 atttgggcaa cgccgtcaac cgaaaccgcc acacggtttt tatctacgcc gcgtatcgag
     2881 tagccgccgc tcgcgccgtt gccctgttcg acaaccgcca cgcccggatc gtagcgcgtc
     2941 aggtcgcgga taccgagtac ctgttctttg ttcaacgttt ccgacgtttt gacgattttg
     3001 cccaaacctg tcgcctcttt cgatcgccgt cccactttgg cggcacggac ggtaacctct
     3061 ttcaatgatt gactttgcgc cgcatcaagt gccgccgccc ccgcttgggc agcataagcc
     3121 ggaaaagcgg ttgcaatggc caacgccgtc aaagtcagcg gaaaaccgtg tttcttattc
     3181 atttttccac ctctttatta tctttcttcg caccgaatac cacgccgaat tggggtttaa
     3241 cttcaggttc taactgttcg ccaacatcag cttcattttc aacttcattt tcaatatctt
     3301 cagttatacc aagagatttc ccatccttat tgaaaatagt gccgcccaat tccgccgcct
     3361 gcgggccgta aaagccgccc gttacaagaa gattgtcggc tttgaaactt tgaggattag
     3421 tcgaaccatt tcccgaaaga ttgatgccgt tatcccgagt gcgtgctgtt gcgtggaaac
     3481 cgttgccctc aatcacgccg ttttcaatac ggaaagcagg ttctacaccg ttttgctccg
     3541 tcagcgttcc ggaaatcgat ttattgccga aatcaacgtc aaattctgct tttgccgctt
     3601 tatccgcctt attatcccat tgaatgggtt cgccgatacg cgcttcccaa gtgccggtat
     3661 aatgcgccgt tccggttttt ggaatgtcgg cttccgccgt gcggatacct ttcaggaaaa
     3721 ggtcgatgtc cctgccttta ggggcttccg gggcgggcgg gatgccgtct gaaacaccgt
     3781 tgccttcttc cggcgattct tcttcgggtt cttcctcttc agcttcttct tcgatttctt
     3841 cagcttcatc agcttcttct tcgacgactt cgtcttcgtc ttcgccgtta tcatcttcgg
     3901 aaacttcgtc ttcgctttct tcggcgattt cttcgtcttc gctttcttca ccgttattaa
     3961 tacccgaatc ctcttcgtcc tgcgccttcg gtttggcggc ggggcgttcg gtttttatcc
     4021 gtccgagttt cacataggtc aaaaagtcgc aacaagcact gacggtcatt ttcctgccgt
     4081 cggcaagatc gatggttttc tcttggctaa ccaaaggaat ttcacgccct tcaacaagaa
     4141 gtttgtcggg atgaccaaaa tcgggcatag gggaaatggc aaacttacgg gcatggttat
     4201 cagtcgcctc gtcaacggaa atttttcgag aatccaagat tttggtgtgt tttccaaacg
     4261 gcatagcagg atttgtgcct gatgcgtttg ttttttgttt gcctgcgaat acgccgaata
     4321 cgccgttgtc gttactgata aaccgtccgg caagctcttc tccgttatcg ccgaaaaaac
     4381 cgccctcaag ccgctgatcg gcatcggtat ggaaaaacaa atattcttta tcagcgtgtc
     4441 tcgtcttcac ctcggtgtta actttggcac tgccggtaaa gcggttgccg tttaaatcgg
     4501 cagtgatgtt ataaatgatc agcggttttt taggttcctt tggatcattt ctcttttgca
     4561 catactgatt tttaatcagc tcgcccttta ggattttttt gtcgaaatca accgtatatt
     4621 cggcaggatg tttttccctg tcgtcggcat ccttagccgc ataagaagtt gcaccaatat
     4681 cgttaccata aaatgttata taacccaaat cgtcactggc aaccgcctta cctgtccgat
     4741 gacgtttggc atcggtcata tattgccagt tgccgaaata ttttaccgtt cccgcgctcg
     4801 gtaaagattg ggaaggacgt tctccggaat aatatacaaa accgtcataa ccaaaacggt
     4861 gggtaaaccg cttaccacct gagttctgtt cgatttcatc ttttccgttt ttagtatata
     4921 cataaccggc atccacaaat ttataattat attttttatt ttccttttct gacgtcctaa
     4981 tttctacatc ggaatcacgt ttatgaattt cgcttttaag ttgttgaagt ttatttcctt
     5041 ttgaaccgta taaaaacaga acatcatctt ctttaaacga cagcttttct tctgcttgtt
     5101 tgctatttgg aaagtcattg ccatccttat cagaagttgc gatattccgc ctcaacagcc
     5161 gcattgccgc accgacggcg ggccggttga ccggcgtggt ttctaccgaa ggctcggcag
     5221 ggggggggga agtgggaatg tccttagact tgaaagtgac ggggtacgcg gtcggcgttg
     5281 attcgacaac aggctgcacg ccgaaattgc cgccgcagga tgccagcatc aacggcagca
     5341 aggcaatacc gtaataattc agtttacgca tccctatccc ccctatttaa ttaataataa
     5401 ttatcattat attaatatgc gcagataata tcaaccagtt tttatagtga attatagtgg
     5461 attaacaaaa atcaggacaa ggcggcgggc cgcaggcagt acagatagta cgaaaaggcg
     5521 aggcaacgcc gtactggttt aaatttaatt cactata
//
LOCUS       DAXFHF010000091         5266 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXFHF010000091 DAXFHF010000000
VERSION     DAXFHF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.091"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(24..200)
                     /locus_tag="ACFIRM_001909"
     CDS             complement(24..200)
                     /locus_tag="ACFIRM_001909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029465.1"
                     /translation="MKSKTEAEKSHLQKVADIGCIVCRNCGRFGVPAEVRHIRNGAGA
                     GCGGVWNHLGCLGV"
     gene            complement(190..570)
                     /locus_tag="ACFIRM_001910"
     CDS             complement(190..570)
                     /locus_tag="ACFIRM_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC3029466.1"
                     /translation="MRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYKETVRRIAQG
                     AGAMPSEGFVAVRLRLIPKANKDGGANKTVIDLDNALKVTLDALQGVAYHNDRQVRRI
                     AADYADEPVAGGGLAVEVGELDEK"
     gene            complement(561..842)
                     /locus_tag="ACFIRM_001911"
     CDS             complement(561..842)
                     /locus_tag="ACFIRM_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029467.1"
                     /translation="MAEEMRTCKACGGTKPLEKGFNAVPRKEGGVYYYKSCKTCRNKA
                     VRQKRAEKRAAAGAGAMTAARLHGYIRAAHAACPILGAGLWTQPAGECA"
     gene            complement(871..1020)
                     /locus_tag="ACFIRM_001912"
     CDS             complement(871..1020)
                     /locus_tag="ACFIRM_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029468.1"
                     /translation="MDTLLSIIIALSFGGAATLAVWLLVEAADAVLRRKRDGKGEDDF
                     DGFGY"
     gene            complement(1334..1828)
                     /locus_tag="ACFIRM_001913"
     CDS             complement(1334..1828)
                     /locus_tag="ACFIRM_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFC3029469.1"
                     /translation="MTVRNTQTETVRTEAAPQQGGNTNPGYYKNREHECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQRAAAPKFKKLKCRRYEEMYAGLKDCGF
                     DCGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETG
                     RGGI"
     gene            complement(1842..2093)
                     /locus_tag="ACFIRM_001914"
     CDS             complement(1842..2093)
                     /locus_tag="ACFIRM_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029470.1"
                     /translation="MRETCFYCNHADFKTNTGTPVRGFAKCAKARNAEEKATYHPRTN
                     PCAAGAFQTASGAAVAKRTAALGEYPGIPEIENRVKTQI"
     gene            complement(2110..3471)
                     /locus_tag="ACFIRM_001915"
     CDS             complement(2110..3471)
                     /locus_tag="ACFIRM_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFC3029471.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAVGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
     gene            complement(3468..3971)
                     /locus_tag="ACFIRM_001916"
     CDS             complement(3468..3971)
                     /locus_tag="ACFIRM_001916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029472.1"
                     /translation="MNADAFVSADAETCGRETGEPTSPKAESDSNGNGGLSGKPKNAN
                     VPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRWCEMLGTAAPNGKV
                     RFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGNFVKILEWHPPKTN
                     QAARGRA"
     gene            complement(4066..4533)
                     /locus_tag="ACFIRM_001917"
     CDS             complement(4066..4533)
                     /locus_tag="ACFIRM_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029473.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLMLQHSGGWGINSPEMQYQQPRTSKSTFGGWGINSPEMQYQQPRTSKSTFGE"
     gene            complement(4651..4878)
                     /locus_tag="ACFIRM_001918"
     CDS             complement(4651..4878)
                     /locus_tag="ACFIRM_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3029474.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            5052..5240
                     /locus_tag="ACFIRM_001919"
     CDS             5052..5240
                     /locus_tag="ACFIRM_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029475.1"
                     /translation="MPSDLSFILAKELIRSGSIRLSGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDDEYLIGLLSK"
BASE COUNT          966 a         1634 c         1310 g         1356 t
ORIGIN      
        1 tatccgacaa tcccgacatc aaatcaaact cctaaacatc ccaaatgatt ccaaactccg
       61 ccgcagcccg cgcctgcacc gtttcggata tggcggacct cggcaggaac gccgaaccgc
      121 ccgcaattgc ggcaaacaat acaacctata tccgccactt tttgcagatg tgatttttcc
      181 gcttcggttt tacttttcat ccaactcccc cacctccacc gccaaaccgc cgcctgcgac
      241 cggctcgtcg gcgtaatcgg cggcaatgcg ccgcacctgc ctgtcgttgt gataggcaac
      301 gccttgaagc gcgtccagag taaccttcag ggcgttgtcc aaatcgatca ccgtcttgtt
      361 tgcgccgccg tctttgttcg ctttgggaat caggcgcagg cgcaccgcca caaagccttc
      421 ggacggcatc gcgcccgccc cttgcgcgat acggcggacg gtttccttat acgccgccgc
      481 ctccgcgctc ctgaccgccc tgttgcgcca aatccgccaa tatcggtttg ccgatacggg
      541 gtaaggcagg ataaggcgta tcacgcgcat tcccctgccg gttgcgtcca caggccggcg
      601 cccaatatcg ggcaggcggc gtgcgcggcg cggatgtatc cgtgcagcct tgccgccgtc
      661 atcgcgccgg ctcccgccgc cgcgcgtttt tccgcgcgct tttgccggac tgccttgttg
      721 cggcaggttt tgcacgattt gtaataatag accccccctt ccttgcgcgg gacggcatta
      781 aaccctttct ccaacggctt ggttccgccg caggccttgc aggttctcat ttcttccgcc
      841 attttgattt ttccttttgt ttttatgtgt ttaatatccg aagccgtcga agtcgtcttc
      901 gcctttgccg tcgcgcttgc ggcgcaaaac cgcgtcggcg gcttccacca aaagccatac
      961 cgccaacgtc gccgcgccgc cgaacgacag cgcgatgatg atgcttaaca gggtatccat
     1021 tgttttattt cctttcggtc ggttttatgg gccgcggtcg gattcccgcc gcctccgtca
     1081 ttcccgccga tttccgtcat tcccgcgaaa gcgggaatcc ggaaaccccg aacgcggcag
     1141 gaatttatcg gaaacggctg aaaccggacg gaccggattc ccgccgattt ccgccaaccc
     1201 cgccgatttc cgtcattccc gcgaaagcgg gaatccggaa accccgaacg cggcaggaat
     1261 ctatcggaaa cggctgaaac cggacggacc ggattcccgc ctgcgcggga atggcggcgg
     1321 tcgggttggc gggtcaaatg ccgccgcgcc ccgtttcagg cggcatcggg ctttcagggt
     1381 gcggggcccg ccccgcttca ggcggcattt tttccaacaa atcttcgaca caggcggccg
     1441 cccacgcaaa attgtcttcg ccgtcgcgga tgtaataagc ggcggagatg acggcaagca
     1501 gcgcggcctc cgtgccgcag tcgaacccgc aatctttcag accggcgtac atttcttcat
     1561 agcggcggca tttgagtttc ttgaacttcg gcgcggcggc gcgttggcgt tccaagtacc
     1621 gcagggcttt ttccaagtct tcgcgcccgc ctttttcctt gtgccgccag atgtatttga
     1681 aggcgttgcc gaggttgaag ttgaggtatt gcgcaaaccc gacgcactcg tgttcgcggt
     1741 ttttgtaata tccggggttg gtattgccgc cttgttgcgg cgcggcttcc gtccggacgg
     1801 tttcggtttg cgtgtttcgg accgtcattc cgtatttcct ttcaaatttg cgttttaacg
     1861 cggttttcaa tctcgggtat gccggggtat tccccaagcg ccgccgtcct ttttgcgact
     1921 gccgcccccg atgccgtctg aaacgccccg gcggcgcagg gatttgtcct cgggtggtac
     1981 gtcgcttttt cctccgcatt ccgcgccttc gcgcatttcg caaaaccgcg caccggcgtg
     2041 ccggtgttgg ttttgaagtc ggcatggttg caatagaaac aggtttcacg catttaccgg
     2101 cctcccgcac tagacactgt aagggtcata ttcatcatgt ttgggggctt gccatgccaa
     2161 atcaggctct tcctcgaact tcataaattg ccctttccag ccgcaaacca ccgttcccgt
     2221 ttcgccgtcc cggttcttgg cgataatcag ctcggcaatg ctcggattct cgtttccgtc
     2281 gtagtagctt tcgcggtgcg gcatgatgat gatgtttgcg tcttgctcaa tcgcgccgct
     2341 gccgcgaatg tctgccatgt tcgggcgttt gtctgcctgc tttgtgtttc ccctgttcaa
     2401 ctgggcaacc aagacgacgg ggatattcag ctctgccgcc aagtttttca aacggcgcga
     2461 tatattcccc aactccgcca cctcgtccct ccctgccctt ggcatgatgt gaaggtgatc
     2521 gacaaccaac aaatccaagc cggtagtgag ttttttctcc ttagccaaaa agcaaagctc
     2581 gtcaacgttg agcaggtcgc agttcacgtc aaacttccac tctttcgctt ggctgacgta
     2641 aatcggcata ttggcgtaat cgctttgcgt cagattgccg gttttcaggt tctgcatggg
     2701 gatattgcac tcagccgcca tgccgcgcct tgccagctct accgcgctca tttcgtagct
     2761 ttggaaatga actgccttgc cctgcttcag cgcgaatcgc gcaatgtttt ccgccaaaac
     2821 cgttttaccc atagacggac gcgccgcaat cacaatcagg tttccatccg gcaaaccgcc
     2881 tgtcatcccg tcaagcttca tcaagccggt aggcaatccg aaacgcacgc cgtcaagcct
     2941 tttgtccaaa ccgccaatca aatcctcaac ggtctggccg aatgtcttgg tttcacgctt
     3001 taccgcgtct ttgccgactg ccgccaattc gtcggccgcc ttagacagct tttctgcgac
     3061 cgtcccgccg tctttggaaa ccgcgatttt ttcaatcgcc gccgaagcct tcagcaagcc
     3121 gcgctcgaca aacctgtcgt tcacaatccc aacgtaccgg ctgatattct tcgcgcttgg
     3181 ggtgttttgg ttcaggtcta tcaagtaagc caggccaccc gcgttttccg cctcgccgcg
     3241 cgcttccagc ttgtcgttca gcgtgatgat gtcgataggc tcgtttgccg ccgccatatc
     3301 cagcagagcg cggaaaataa tcctgtgttg cgcctggtaa aacttttcag gggtcaggat
     3361 tgcgcaccgc gcaatcgccg tcggttcaat caagatgccg cccaaaatgt tctgttccgc
     3421 ccctacgctg gccagtgatt ggaccgcttc cgtttcctcg attcggttca tgcccttccc
     3481 cttgccgcct ggttcgtttt aggcggatgc cattcaagga ttttgacgaa attgcccgcc
     3541 ttgaaaatcc aatcgaagcc gaccgcaaat cctgtttggt tttcgcccat ccaaaacggg
     3601 ttcatcgcca ctttccggaa gaaaccggca aaccaggcca aaccggtttc cttgtccccg
     3661 aacctcacct tgccgtttgg cgccgccgtt cccagcatct cgcaccagcg gtttgcaatc
     3721 gcccgtttgc gcgtgtcgtt cagcacttgg acgcttggca accggccgcc caaaacttcg
     3781 ttgtacaaat cggcgatttc ctgaagcggt acgccgtggg ttttgcggcg gcgcggaaca
     3841 ttcgcatttt tcggttttcc cgaaaggccg ccgttgccgt tactgtcgct ttcggccttc
     3901 ggcgaagtcg gttcgccggt ttcccgcccg cacgtttccg cgtcagcgga aacaaacgcg
     3961 tcagcgttca aaccgccttt gccgtttccg gcgtttgggg gtaagggggt atatactgct
     4021 aaatcttctg ttaaatcttc tgttcttata acggctgttg attcctcact ccccgaatgt
     4081 tgattttgaa gttcggggct gttgatattg catttcgggg ctgttgattc cccacccccc
     4141 gaatgttgat tttgaagttc ggggctgttg atattgcatt tcggggctgt tgattcccca
     4201 cccccccgaa tgttgcaaca tcaaatcatc aaaagcgtct aaattcagtt tgtagtaaat
     4261 gcggtgttca attcgttttt cagtctcaat caatacgccg cgttccctca gctttgcccg
     4321 tgccgttctt tgttcctgaa cggacagccc ggtttcaatt tcaatttctt ccgctgttcg
     4381 gtaaatgcct gattcatact gcgtcttatc gttccagtag aagaaatgcc cgaataatat
     4441 tgccgcattt acaccgccca aaggctttgc caatttcggg taatacgcga ttggcctgcc
     4501 tgccgctctc aaactttcag acggcttcat catcaacccc tttcacttct tcgacccgcc
     4561 cgtccaacgc ttcccgtgcc ttgcccgcgt cttccgcctg catatgagcc ggcaccggca
     4621 agcgggctcc gcgtattctt tgttcccggc tcatggttca attcctgact ttgcgattga
     4681 gtaatgggca acgggatttt tacagttacc gaccttgtat ttcggcttgg caaacacgaa
     4741 gcccctgcct tccaagtcgg ttatccgtgc cgcaagctgc gtcaccttca ggttctgata
     4801 agcctcaagg gatgtgatgc atcccttgtt acggatgtaa tcgacaattt gtttgcattg
     4861 cgtttgtttt tgattcataa tcgcctttcg ccgttacctg aaccgcttcc ctgcaattca
     4921 ggggggatta cccgccccgt gcgggttttt ctttatcggc cgcccgtctg tccgggcggt
     4981 cagccgtctt tccgatttgc catcaccccg ttgcaaccgg gtttccacac aacggccgac
     5041 ggagtaaaaa aatgccgtcc gacttatcct tcatactcgc caaagagctt atccgcagcg
     5101 gttctatccg cttaagcggc agtacggcca aaggacaagc cggagaattg gcggtattca
     5161 tccgaacact tcatcaaaaa cccgaagaat cggagccaaa taccgatgac gaatatttaa
     5221 tcgggctgct ttccaagtaa ttcaaagccg tctttcaaac ccggcg
//
LOCUS       DAXFHF010000092         5013 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXFHF010000092 DAXFHF010000000
VERSION     DAXFHF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.092"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..662)
                     /locus_tag="ACFIRM_001920"
     CDS             complement(<1..662)
                     /locus_tag="ACFIRM_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029476.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKVLSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRGGKPVRDFDAQRPLVSSKPDALSPEE
                     RELYKRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REA"
     gene            complement(899..3166)
                     /locus_tag="ACFIRM_001921"
     CDS             complement(899..3166)
                     /locus_tag="ACFIRM_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029477.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVDEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGAVLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFP
                     YVPFFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQ
                     FRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPS
                     FMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTV
                     VIEGDAITQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGA
                     MSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIAQAVQD
                     ALAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAGGQIAAMPQIAP
                     VADAVMQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSAN
                     HAQTGMETPTVSDNL"
     gene            complement(3151..4425)
                     /locus_tag="ACFIRM_001922"
     CDS             complement(3151..4425)
                     /locus_tag="ACFIRM_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC3029478.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCAREIQKSMRDSVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMSKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     AAGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(4406..4945)
                     /locus_tag="ACFIRM_001923"
     CDS             complement(4406..4945)
                     /locus_tag="ACFIRM_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029479.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYAGFNLKQVTDAEAKRVAAVQRRHRNLAE
                     NLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIERKSYGMDTAESKISESA
                     KTAGIRIEFVGPEDDGKDG"
BASE COUNT          965 a         1634 c         1273 g         1141 t
ORIGIN      
        1 gcctcacggt tcgccgcatt ctccgccgca gccgccgcca gcctttccct gcccaattcc
       61 ctgaccgccg catttttatt atgttccgcc acaatcccgc gcgccgcatt aagctgcgcc
      121 gccgtcaact gaccgttcaa ggcccccgca tgaggcgtag tcgccctctt ataaagctcc
      181 cgctcctcag gcgacagcgc atccggcttg gaagacacca aaggacgctg cgcgtcaaaa
      241 tcacgcaccg gcttgccgcc ccgatacaca ttcccgtcaa atcccaaagg cggcggcgtc
      301 cgtccgcccg catccgtcgg gcgcgtatcg cgccgcgcga aatccgcccc ggcccgcgca
      361 tcgggggaaa gcaccttatc gccgcccgaa aaccccaaac cggcatccgc accccgttcg
      421 aaagcacccg cccggcgcaa ccgccgcgcc ccatcctcaa tattgttacg gctcaaatcc
      481 atcacattcc gataacgcgc cggcccggcc tcataattcg caacggtacg ccgcaccgca
      541 tcggcacggt gcgcctgtgc cgccgcccgc tcccgctcct ccgccccctt ccgggccttc
      601 tcttttttct ccttttcctt catctcccaa aaatccccgg cagacggctc aaacaaaccc
      661 atcgcaaacc ctccgaacaa ataaagccca aaataagcaa aaacccaaac ccaatcaaac
      721 gcctaaagtt aagaacaaac aaaaatcgca accacaaacc aattaataaa caacaagtta
      781 cacaaaaaaa cacaccgacg ggccatttcc gggggcaaat aaaaaccgcc ttcgtgcaag
      841 gcggttgcaa aaaagccttt catttaacga aaggcttttt gccgcccgcg taccgcgttc
      901 agaggttgtc cgacaccgtc ggcgtttcca tgcctgtttg ggcgtgattc gcactctttg
      961 gcggcacggc cgtcatcggg ccggtatccg cgccgtaggc ttcagggatg ccttcgggcg
     1021 gtatttgcgt ttcaggcggc atggcgggga cggggaagcc gggatcgtcg ccccgcgccg
     1081 gcctgacata tcccgcgccc tgcatgaccg cgtcggcaac gggggcgatt tgcggcatgg
     1141 cggctatctg cccgcccgcc tgcatggccg cataagccgc ctgcacgccg atttgtaccg
     1201 cccgcgcttc gatttccttg atttcgctcg cggtacgttg ttccttgagc gccaattccc
     1261 gccgtttgat gtcgttgccg gattttgcca atgcgtcctg caccgcctgc gcgatacgcg
     1321 cctcgatttg ttcgggcgtt tcctgcactc ggacttcttt gattttttca atcactttgt
     1381 ctttaaacgg gatgtccatc agggacacca taaacggcag caccgccgcc tgatattcgg
     1441 gcggcaggga tttgaccgcc tcgctcatcg cacccagctg ctggctgcgg taggagttgg
     1501 tgctgggcac gtcttccaaa gccactttca gccgtatgtt ttgcaggtcg ttggacaaat
     1561 aagccttgcc ggttacgggg tcggtttcag gcctgttgat gacgaccgtc cgcccttgcg
     1621 tgattgcgtc cccttctatg acgacggttt gctcgtccga gcccaaatcc tcgataatca
     1681 tcgcaagcag caactcgccg accaatgagc ggctctggcg gaagttgtcc ataaccagcc
     1741 ccagcgactg gttggactgc tcgacttgga tgctttcctg cctgccgctg gtggcgttgc
     1801 cccggttgcc cataaatgac ggggtaatcc cgctgatttg ccgtatggtc gcgcggctgt
     1861 cttgaagcat ctgccaatgc tgtgccgaca attcgaaatc gcggctgacg tcgaaacgcg
     1921 cgcccggctg ggcggcctct attttgttca gcacgatgtc cgcgtccacc cgtgcgatat
     1981 tgcggcggaa ctgttcgtcc gacatatcga ctatgccttt ggtgcgtacc gtgcgtattg
     2041 ccgacaaacc ccatcgtaat ttgctgttgg tgctgttgag gttgtcctgc gcgtatttca
     2101 tattgcggac atatccgtag gggatgccgg tgttgtcctc gcggaatccg aaaaacggga
     2161 cgtaagggaa cttttgatgc ggatacgggg tcggctcgtc gcggacgacc aaatccccca
     2221 cgacaaaggc gcggcgcata cgcgggacgg atgcggcaaa caataccgcg ccgtttgccg
     2281 ccatctcccg atggtttggg tttgcgccgt caaactccac cgtgcgccct gtttttttgt
     2341 cgcgcaggca gtctgccgtt acccaccgcc tgtaccatac ttccgccacc gccagttcgc
     2401 gcgtggtttc attgaaccaa aactcttcgc tgacggtgtt gcgccccgaa ataccccaag
     2461 cgtccgccag tccggtattg ccgccgccgt caagcatttc cccgctgatg cgccagtccg
     2521 aaccgccgcg ccccatcgct ttgaagtgtc cggcatattc agggaagaat tgcgccaggc
     2581 gttccggcag cagccagcgg cggcgtatca gccagcgggc atcggacagg tcgtatttgt
     2641 aagacttcat atcccaatgg atggcgttgc ggtggatgac gccgcactca taaggaaact
     2701 cgaaagggtt ggggttgcgt gtaacctcca cccagccgat gccgcaggct atctgccccc
     2761 tgaacgcgtc cgaacaagcc ttgtcggcac ggctttgccg ttccgcgcgg ttgagtttga
     2821 agttcaatgc gtccgccacg tcccgcccgc cggtttcgcc gtccgccgtc acgcgccagt
     2881 ccgtccgtat cgtcgcctca tatccctgta ccgacagcag ggtcggggta atcaggtttt
     2941 caacggcggg gggcaggccc aactcgcgct gtttctgtaa aagctcgttg tcaagctgcc
     3001 tgccgtcggc gtagtccatt tccttgtcgg caacggcacg ccatgcaggt tggttcatca
     3061 tttcatcgac aaacagccgg tattccccga tagtcagcgg ttcgccgttt ttatcgggca
     3121 atacgcctgt ttccggtacg tccgtcccca tcaataaagc ctccaatccg aaacgggcgt
     3181 ttccgtgtat cccgtattgc tgcctgtatc cattaaatca accgcctgcg ccaggtagcg
     3241 gaacatatcc gcgccgtgcg aatattcgtc gtgcagcggc cccattgcca cgcctgtttt
     3301 cgcatgtatt tggcggccgt accgtttcag gcattccaac agacgtgcag ttttgtcttt
     3361 atcgaagtac actttgggaa acagcatccg cgccgccctg atgccttctt cgataccggc
     3421 ggcgttttgc acgaaaaccg acttgcgccc cagtccggtc aaaatctcca tcgtactttt
     3481 gcctgtttgg aagttgcgcg tcctgccgtc gtgcggcagg aagtccgtcc cccaccggta
     3541 gggcagcttt tccaattcgg caacatacca gtccaacgtc cggtgcgtgt cttcgatgta
     3601 gccgatgatg cgcacgctcg tcaaatcgcg ctgcaccagc ccgatggtca tggcatcgtt
     3661 ccagcccaaa tcccaaaccg tatgcacggg cagggaagaa tcataaggga cgagcgtaac
     3721 gcgtccggaa tgaaaagcgt cctgtatttc atgccggtaa accgccccct ccgataccat
     3781 gcgcggcctg ccttcccaaa tattcccgta gtcctcttta ctcatcgaac gctgtgcttt
     3841 gagccgctcc cggttcaatg cttcggggaa ccacggattg tcgcgccagt tgatttcgca
     3901 caaccaagtg tcttcggacg gcatagcgat aaaacgccgg taggtttcgt ccgtctccat
     3961 atcgggattg agggtaatcc aaatttccga accttctttg cgtatggtcg gcgtgagcac
     4021 gtcccagctt tttttactga cgccgtgccc ttcctcaacc catacgatgt cgataccttc
     4081 aaacgatttg atgctgtcca cggtatgcga ctgcaggccc gaaaacacga acagcgtgcc
     4141 gtttgcgccg cgtatctcga agtcggttat ttcgtagaaa tgccccaaac ccaactgcgc
     4201 cactttgtct ttcaacaggc ggtgtacgga atcgcgcatc gatttttgaa tttcgcgcgc
     4261 gcataaaata cgcaaagggc gttgcgcccc cagcgccagc agtgcggatg ccgcgccgtg
     4321 cgatttgccg ccgccgcgcc cgccgtacat aaccttgtac cggcaaggct tgaacagccc
     4381 gtccagtttt gcgggcaatt tcaaatcaac cgtctttccc gtcatcttca ggccctacaa
     4441 attcgatacg gatgcccgcc gtcttggcgg attccgaaat cttcgattcc gcggtatcca
     4501 taccgtatga tttacgctcg atttccacca aaaccttaaa ccccgccgcc accgtttgga
     4561 acatacgcgc ctttgtgaat gtgtcctccg ggcttccgta caattccgcg acaatctgct
     4621 ccgcgctttt ggacaggttt tccgccaaat tccgatgcct gcgctgtacg gcggcaaccc
     4681 gtttcgcttc cgcgtccgtt acttgtttaa ggttgaagcc cgcataatcc ggcgcattgc
     4741 cgtttttgtc tgcggacagc accatcgccc gcgcctttgc ctgtacctcg tcattcaaat
     4801 cgcgcgtcca cacgcgttct ttggcaaccc tgccgacgtg ttgcgccgaa acattgtatt
     4861 tgcggccgat ttcggcaatg ctcaaaaccc cgcgccggta atccagctcg actttgcgcc
     4921 aatcttcatc tgacaatctc ggcattaaag gtccaacctc ccgtttttgg cggaacattc
     4981 cagataattc cgcaatactg aaaactcgcg ccc
//
LOCUS       DAXFHF010000093         4676 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXFHF010000093 DAXFHF010000000
VERSION     DAXFHF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.093"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..191
                     /locus_tag="ACFIRM_001924"
     CDS             <1..191
                     /locus_tag="ACFIRM_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029480.1"
                     /translation="LRAEQSVQAPFETRQISYAWRESGSPEETVLTAQTADSPDLPVQ
                     AVLNLTHRSHNGKPLILP"
     gene            252..1286
                     /gene="pyrC"
                     /locus_tag="ACFIRM_001925"
     CDS             252..1286
                     /gene="pyrC"
                     /locus_tag="ACFIRM_001925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC3029481.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="ACFIRM_001926"
     CDS             complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="ACFIRM_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC3029482.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(1868..2344)
                     /gene="ribH"
                     /locus_tag="ACFIRM_001927"
     CDS             complement(1868..2344)
                     /gene="ribH"
                     /locus_tag="ACFIRM_001927"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC3029483.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2391..2714)
                     /locus_tag="ACFIRM_001928"
     CDS             complement(2391..2714)
                     /locus_tag="ACFIRM_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC3029484.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2881..3600
                     /gene="rnc"
                     /locus_tag="ACFIRM_001929"
     CDS             2881..3600
                     /gene="rnc"
                     /locus_tag="ACFIRM_001929"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC3029485.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSSGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHADDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3647..4570
                     /gene="era"
                     /locus_tag="ACFIRM_001930"
     CDS             3647..4570
                     /gene="era"
                     /locus_tag="ACFIRM_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC3029486.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         1003 a         1174 c         1436 g         1063 t
ORIGIN      
        1 aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac caggcaaatc agctatgcct
       61 ggcgggaatc gggcagccct gaagaaaccg tattgaccgc acaaaccgcc gattccccgg
      121 atttacccgt acaggcagta ttgaacctaa cgcaccgttc ccataacggg aaacccctaa
      181 ttctcccctg acttcagacg gcataaagtc ggcatgccgt ctggaaacca aaaatctaaa
      241 aaggaacaac catgcaaacc ctgaccatca tccgacccga cgatatgcac ctgcacctgc
      301 gcgacggcga cgcgctcaaa gccgttgccc cttataccgc ccgccagatg gggcgcgccg
      361 tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc cgacgcgctt gcctacaaag
      421 cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga gccgctgatg acgctttacc
      481 tgaccgacca agccacgccc gaactcgtgc gcgaagccaa agccgccggc atcgtcgcct
      541 tcaaactcta ccccgcaggc gcgaccacca attccgattc cggcgtaacc gacctgttca
      601 agctcatccc cgtgttggaa gaaatggcaa aacagggcat cctgttcctc gttcacggcg
      661 aagtaaccga ccccgaaatc gacattttcg accgcgaagc cgcctttatc gggcgcgtga
      721 tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt gttcgaacac atcaccaccg
      781 ccgaagccgc ccgcctcgtt ttggaagccg gcgacaacgt tgccgccacc gttaccccgc
      841 aacacctcct gctcaaccgc aacgacctct tggtcggcgg cgtgcgcccc catcatttct
      901 gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt ggtcgccgcc gttaccggcg
      961 agaaggcgca caaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag
     1021 aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac cgctatcgag ctttacgccg
     1081 aagtgtttga aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg aaaaacggcg
     1141 caaggttcta cggcattccg gaaaacgccg acacgattac ccttgtcaaa caaagccaaa
     1201 ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg
     1261 aaatcggctg gacggtgcag tattgattga acggtcaagc aaaatgccgt ctgaaaggtt
     1321 ttcagacggc atttgtgtat tttccgattc ggattttccg agtatcaacg gcgtttgggc
     1381 tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc cgttgacgaa tttgtgcccg
     1441 tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt tgataatgac ggggtagggc
     1501 gtttcgggca tagcggaaag ctcgtggcag gcggtcagca aaacggcgcg ttcgatgggg
     1561 ttgaggtctt tttcgtccct gtcgagcagc gggcggattt tttggatgta gtccgctgca
     1621 ttggtttgtg tgccgaagaa gagtttgttg aacaattctt cgtccgcttt ggcaaagtcg
     1681 gacatttcgc ggatgttttt agcaatttcg ggcgcggcgg tgcggttgat aagggattgg
     1741 taaacggctt gtacggcaag ctcgcgggaa cggcggcggg ctgttttcat gatttttcct
     1801 tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa agggcgcatc ggtgtacgcc
     1861 ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag aaggttgacg aggttggcgc
     1921 attctacggc gactttggcg gcatccgagg ctttttctcc aatccgttca attgcctgcg
     1981 cgtcgttttc ggtggtcagg acggcgttgg caatcgggat gttgtagtcg agtgcgacgc
     2041 ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa atggtaggtt tcgccacgga
     2101 tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga agaggcaaag ttcatcagcg
     2161 cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt gatgttttcg tctgctacgc
     2221 ccaattcttg gagggtgcgg cagcagactt tgagcatttg gctgccgatt tcgttggtga
     2281 agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc gaggttgggg gcgatggtgt
     2341 tcattgggtg tcctttggta ttcggggttt cggaatgccg tctgaaggtt tcagtcttgc
     2401 ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg caagctccca cgcgctgcct
     2461 tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggtgatgtc ggcgaggctg
     2521 acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg tttcgtcgcc gctgaagaaa
     2581 cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt aaaggaagcc gacgggccgg
     2641 ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga gtgccgagga aagtgcttgt
     2701 gcaaatgcgt tcattgcggg aatacgttgg gggggaactt gcggatttta ccacgattcc
     2761 cgcgttgtcg gcagacggcg gcggtttggt ggtacaatgt gcgccgtttg cagccttaag
     2821 gtgtttctgt atttttggag tatggaaacg cattcgggct gttttttgcg gaagacggta
     2881 atgaaagacg atgttttgaa acggcaggca catactgcga tacagaaaaa gttaggctac
     2941 gcgttccgcg atatgtcgct tttgcggcgg gctttgaccc acaggagcca tcatgcgaag
     3001 cacaacgaac ggttcgagtt tgtcggcgat tcgattttga attatacggt ggcgcggatg
     3061 ctgtttgacg cgtttccgaa gttgaccgag ggcgagttgt cgcggttgag ggcgagtctg
     3121 gtcaatgagg gcgtgctggc ggaaatggcg gcggaaatga atgtcggcga cggtctgtat
     3181 ttgggggcgg gcgagttgaa gagcagcggc ttcagacggc cttcgatact ggcggacgcg
     3241 atggaggcga tgtttgccgc cgtcagcttc gatgccgatt tcaacacggc ggaaaaggtg
     3301 gtgcgccatt tgtttgcaga acgcgtccgg cgcgccgatt tccaaaatca ggcaaaagac
     3361 ggcaaaactg ctttgcagga ggcgttgcag gcgcgccgtt tcgccttgcc gaaataccgc
     3421 atcgaagagc aaatcggcca tgccgacgac agtatgtttg tcatttcctg cgatttgggc
     3481 gaactgggtt tcgtgtgccg tgccaaaggg acgagccgca aggcggcgga acaggaggcg
     3541 gcgaaagagg ctttgaaatg gctggaagag aagctgccgc tgaagaagaa aaagaaatga
     3601 ggcggcgcgt gaatatgccg tctgaaatgt ggatatgaaa gcgaatatgg atattgaaac
     3661 cttccttgca ggggaacgcg ccgccggcgg ataccgttgc ggcttcgtgg cgattgtcgg
     3721 tcgtccgaac gtgggcaaat caacgctgat gaaccatctc atcggtcaga aaatcagtat
     3781 taccagcaaa aaggcgcaga cgacgcgcaa ccgcgtaacg gggatttata ccgacgatac
     3841 cgcgcagttc gtgtttgtcg atacgccggg ctttcaaacc gaccaccgca acgcgctcaa
     3901 cgacaggctg aatcaaaatg ttaccgaggc gctcggcggt gtggatgtgg tggttttcgt
     3961 cgtggaggcg atgcgcctta ccgatgccga ccgcgtcgtg ttgaaacaac tgcccaagca
     4021 cacgccggtc attttagtga tcaacaaaat cgacaaggac aaggcgaaag accgttacgc
     4081 gctggaggcg tttgttgccc aagtgcgcgc cgaatttgaa tttgcggcgg cggaggcggt
     4141 cagtgcgaaa cacggtttgc ggattgccaa cctgttggag ctgctcaagc cgtatctgcc
     4201 cgaaagcgta ccgatgtatc ccgaagacat ggttacggac aaatcggcgc gttttttggc
     4261 gatggaaatc gtgcgtgaaa aactcttccg ctatttgggc gaggagctgc cttatgcgat
     4321 gaacgtcgaa gtggagcagt ttgaagaggg agacggtttg aaccgcatct acatcgccgt
     4381 tttggtcgac aaagaaagcc aaaaggcgat tttgatcggt aaaggcgggg agcgtttgaa
     4441 aaaaatttcc accgaagcgc ggctggatat ggaaaaactg tttgataaca aagtattttt
     4501 gaaggtctgg gtcaaagtca aatccggttg ggcagacgac attcgcttcc tgcgcgagct
     4561 gggtttgtag tttttcttgc tgaactttac gcaaatgccg tctgaacagg tttcggacgg
     4621 cattttgttt caatcgggaa tatctttgtt aaaaacgggt tgatattatc tgtgca
//
LOCUS       DAXFHF010000094         4551 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXFHF010000094 DAXFHF010000000
VERSION     DAXFHF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.094"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..667
                     /locus_tag="ACFIRM_001931"
     CDS             <1..667
                     /locus_tag="ACFIRM_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC3029487.1"
                     /translation="AHIQAGDRVLSKDEASGETGYKPVTARYGNPYQETVYIEVSDGI
                     GKIQTLVSNKIHPFYSQGKWIQASRLKKGDTLLSESGAKQTVQNITLKQQPLKAYNLT
                     VAGWHTYFVKGNQAETEGVWVHNACPPYKRPNNATTKAQRESVQGKPCVECGKLAEKM
                     IVDHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQYSKEQKRKLSNG
                     NKK"
     gene            651..1004
                     /locus_tag="ACFIRM_001932"
     CDS             651..1004
                     /locus_tag="ACFIRM_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029488.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSHDENFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            994..1317
                     /locus_tag="ACFIRM_001933"
     CDS             994..1317
                     /locus_tag="ACFIRM_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC3029489.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIDGSQKNLQSIES
                     QIISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1376..1603
                     /locus_tag="ACFIRM_001934"
     CDS             1376..1603
                     /locus_tag="ACFIRM_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029490.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1730..2273
                     /locus_tag="ACFIRM_001935"
                     /pseudo
     CDS             1730..2273
                     /locus_tag="ACFIRM_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            2375..3139
                     /locus_tag="ACFIRM_001936"
                     /pseudo
     CDS             2375..3139
                     /locus_tag="ACFIRM_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            3136..3471
                     /locus_tag="ACFIRM_001937"
     CDS             3136..3471
                     /locus_tag="ACFIRM_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029491.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            3519..4043
                     /gene="mafB"
                     /locus_tag="ACFIRM_001938"
                     /pseudo
     CDS             3519..4043
                     /gene="mafB"
                     /locus_tag="ACFIRM_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            4021..4302
                     /locus_tag="ACFIRM_001939"
     CDS             4021..4302
                     /locus_tag="ACFIRM_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029492.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            4358..4551
                     /locus_tag="ACFIRM_001940"
                     /pseudo
     CDS             4358..4551
                     /locus_tag="ACFIRM_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         1511 a          869 c          966 g         1205 t
ORIGIN      
        1 tgcccatatt caagccggag accgcgtcct ttccaaggac gaggcaagcg gggagacggg
       61 atacaaaccc gttaccgccc gatacggtaa tccgtaccaa gaaaccgttt acattgaagt
      121 ttcagacggc atcggtaaaa tacagaccct cgtttccaat aaaatccacc cgttttacag
      181 tcaaggaaaa tggatacagg caagtcgtct gaaaaaaggc gacaccctgc tttccgaaag
      241 cggtgcaaaa cagacggttc aaaacattac tctcaaacag cagccgctca aagcctacaa
      301 tctgaccgtt gccggctggc atacttactt cgtcaagggc aatcaggcgg aaacggaagg
      361 ggtttgggtt cataatgcgt gtccgccata taagagacca aataacgcaa cgacaaaagc
      421 gcaacgagaa agtgtacaag gaaaaccctg cgttgaatgc ggtaaattag ccgaaaaaat
      481 gattgtcgac cataaaaaac cattagttgt cgagtattat gaaacaggaa caattgacaa
      541 atctaagatg agagcaattg aatcagtaca accgcaatgt ccgacttgtt ctgcaaaaca
      601 aggagggaga ttaagccaat attcaaaaga acagaaaagg aaactgagta atgggaataa
      661 aaaatgaaca aataataatt tgtaaaaaat ataatacaga aatatatcct gtttccgatg
      721 tttcaaaaat cggtgttgct gaaaatgtca aacaggcaaa cctgtatccg attaacgggt
      781 taaggcaccg tcccaaaggt gatactaacg gttggtatat ctgggccggt gaaaacttct
      841 cccatgatga aaattttttc ctgcccctgc atactttcca tttgcaaata tggcgacccg
      901 aaatcatccc ctttttgaca cttccgcccg gttaccgttt tttaattggg gaaaacggtt
      961 atgaagatgt atggtttgat gagttgctat taaatgataa ttgacgaaaa atctacttca
     1021 gatgtactga aacaatatca agaactaggg tttgatttga caaaacctat gataatagag
     1081 ttttttatag atggttctca aaagaatctg caatcaatag aaagtcaaat catttcttat
     1141 cggcaagatt ttcaaatcag tattgaacaa gatgaattcg gagaaatgtg gacttgctat
     1201 tgttccgtca atatcgtccc ttcccttgaa aacattttag ctattgaaac tacgttattt
     1261 ggtattgcac aaaaaaatga ttgtaattat gaaggttttg gttcgtatgg gaactaacaa
     1321 aaagtgtagg ttgggttgaa aaaaccatat tcccattaaa gattagggga tgtctatgga
     1381 aataaaattc aatactttgg gggttatcct gaatggggtc aatcctgaag aaaaattcat
     1441 aaaaattatt gatgaccagg aaaataccgg tggatttctg atactgttat cttcaaatga
     1501 caaattctca tcatttgaca gttacgatga ctgggtcgaa aacttagaaa tattaaaaga
     1561 atacctgcaa gaatctcatt ggataataaa gtgggtcgga tagtcggcaa acgtagtccg
     1621 tttgaaacgc ttttactttt cagacggact ttctaccaat gggaacaagg cgttttgtct
     1681 caatccggtt tatgattatt cgatacagtg atgggaagct aagaaaaata tgattaatca
     1741 tattgaaaaa tatataggca ctatttctca tggctcgaaa agcgattcgg gaagtcagta
     1801 caaattaaat atcgcagcta ttccctcttc ccctaacagg gatttaaaaa cttatattac
     1861 tttagggtta agcaaacatg atctgaatta taaaagcaga tttgaaatac tttttgtatg
     1921 ttctttaaaa tatgatgaaa atcagatttt cccattttta agatggttag cagaaaccat
     1981 cattgagaat aaaaaaaatc cttcttcggg ggcaggttgt ttatttgcct agaagcattg
     2041 ttaattcaac aaaaatggac gcactgtatg tttccgcccc attttatttc gacgatgatt
     2101 tccaagtctg ttatggcgaa cactacaata ttgttttccc tttgcttgtc cccttgtata
     2161 aacaggaagc cgaattggtg gaaaaaaagg gttggaatgc ttttgagcag ttcttgttgg
     2221 ataatgaagt tggcaacctt tcggatatga ataggaaacc gtttgtttgg taagggcttt
     2281 cttaaatttg attaaaggtc gtctgaaatt aagcttgttt ttcagacggt ctttccttca
     2341 tttgaaacag aatattgaaa actaagttct tcaaaaatcc tacacctgct ctttccacgg
     2401 cagcaccttg gtcagaacgg cagacggcta caaagccatc gcccatattc aagccggaga
     2461 ccgcgtcctt tccaaggacg aggcaagcgg agaaacggga tacaaacccg ttaccgcccg
     2521 atacggtaat ccgtatcggg aaaccgttta cattaaagtt tcagacggca tcggcaacag
     2581 ccaaaccctg atttccaacc gcatccatcc gttttacagt caaggaaaat ggatacaggc
     2641 aggtcgtctg aaaaaaggcg acaccctgct ttccgaaagc ggtgcaaaac agacggttca
     2701 aaacattacc ctcaaacagc agccgctcaa agcctacaat ctgaccgttg ccgactggca
     2761 tacttacttc gtcaagggca gtcaggcgga aacggaaggg gtttgggttc ataatgagtg
     2821 tccgtatgga aaaggcaacc aacgatataa agatgctcct tatcatggta aaaatgataa
     2881 ttctgtgaaa agtagagcgc caacaaatgg gcaagctgtc cttgataact ctgttcaagt
     2941 taaatcgaca tcttctcaaa gagttggagt tgataaaacc aataatgaaa tcgttgtatt
     3001 aaaccaaact agaattttta atgacggttc tgctgagtat catgggcatg taaggaactg
     3061 gaaaaattta catactgatc aacaaaatgc tttaaagaaa gcaggattgg ttaattcaaa
     3121 aggaaaaatc aaaaaatgat ggctgataag gaacaagtag aaaagatgat ttattctgaa
     3181 aataaagatt gtgttattga tggtatgctc ggtatgacct tcagctcgga ggaggatgaa
     3241 atcccttgga tttccgagaa attgacggag ttgtcgaagc ataaagactt ggatattgcc
     3301 agattgtcat taacctgttt cggacatctc gccaggatgc atgaaaatat cggcgattgc
     3361 gataaagtga ttgctttatt gcgctcaaaa caaggtaatc cggattttca aggttttgcc
     3421 gaagatgcgt tagacgatat ttccttcttt atatttaaaa agcgtccgta agcgcggatt
     3481 aaacccggaa atccgatgcg tctgaaagta cattccggct ttcagacggc atcggtaaca
     3541 gccaaaccct gatttccaac cgcatccatc cgttttattc ggacggcaaa tggattaaag
     3601 cggaagattt gaaagcggga agcaggctgt ttgctgaaag cggtgcaaaa cagacggttc
     3661 aaaacattac cctcaaacag cagccgctca aagcctacaa cctgaccgtt gccgactggc
     3721 atacctattt cgtcaagggt gatagggcgg aaacggaagg agtttgggtt cataatgatt
     3781 gtccgcctaa accaaaacca accaatcatg cccaacaaag aaaagaagaa gctaaaaacg
     3841 attctcatcg aagtgtggga gattccaatc gtgtcgttcg cgaaggaaag caatatttag
     3901 attccgacac aggaaaccat gtttatgtaa aaggagataa agtggttatt ctaactcctg
     3961 atggaagaca ggtaactcaa tttaagaact cgaaagccaa tacgtcaaaa agggtaaaaa
     4021 atgggaaatg gacaccaaaa taatcaatct tccttagaaa aaagaatttt ttatctggag
     4081 cattcggggc agtatctgat gatttgcgcc ctatcggatt actcccaaaa taaacatact
     4141 gtcgttatgg cgaatttcct ctatccaaat gaaaaaatgg attggagaaa tttagatgat
     4201 ttgttcaatg agttggtttt agaggaatta cagtcttcat ttatggattg gtatccaact
     4261 attgaaaagg caattagcca tcatttagaa gacttttcgt aacaagcata aatcaaaaag
     4321 tcgtctgaaa ctgcttttaa ttttcagacg acctttcccc atatcggcgg attatgaatt
     4381 tacagctatc gggaaattga gttatctctc tgatagagaa aattccatac gcgaagccgt
     4441 tgaccggtgg atacaggaaa atcccaatgc cgccgaaacc gtcgaagccg tcttcaacgt
     4501 tgccgccgcc gccaaagtcg cgaagctggc aaaggcggcg aaaccgggga a
//
LOCUS       DAXFHF010000095         4550 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXFHF010000095 DAXFHF010000000
VERSION     DAXFHF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.095"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            120..356
                     /gene="acpP"
                     /locus_tag="ACFIRM_001941"
     CDS             120..356
                     /gene="acpP"
                     /locus_tag="ACFIRM_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC3029493.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            514..1761
                     /gene="fabF"
                     /locus_tag="ACFIRM_001942"
     CDS             514..1761
                     /gene="fabF"
                     /locus_tag="ACFIRM_001942"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC3029494.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            2154..3379
                     /locus_tag="ACFIRM_001943"
                     /pseudo
     CDS             2154..3379
                     /locus_tag="ACFIRM_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3384..3506
                     /locus_tag="ACFIRM_001944"
     CDS             3384..3506
                     /locus_tag="ACFIRM_001944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029495.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            3733..>4550
                     /locus_tag="ACFIRM_001945"
     CDS             3733..>4550
                     /locus_tag="ACFIRM_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3029496.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGVNPFSDIKSTFLIFKALKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEMI"
BASE COUNT         1113 a         1378 c         1105 g          954 t
ORIGIN      
        1 caaatccgca aatattgata taataggcat tagatagaaa actctattaa aaatcggtac
       61 aataccaccg atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata
      121 tgtcaaacat cgaacaacaa gttaagaaaa ttattgctga acaactgggc gtaaacgaag
      181 ccgacgtgaa aaacgaatct tccttccaag acgacttggg cgcggattct ttggataccg
      241 tggagctggt tatggctttg gaagaagcct tcggctgcga aatccccgac gaagatgccg
      301 aaaaaatcac caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg
      361 tcgtcgcccg acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg
      421 caaactgttt gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc
      481 ccccccattc cgacaaccaa cagcgaggtt atcatgagtc agagaagagt agtcattaca
      541 ggcttaggtc aggtttcccc tgtcggcaac actgccgcag aggcttggga caacctgctc
      601 gccggcaaaa gcggcatcgg cgcgattacc cgctttgacg catccgacat caacagccgt
      661 gtcgccggcg aagtgcgcgg tttcgacatc ggacaataca tcagcgcgaa agaagcgcgc
      721 cggatggacg tgttcatcca ctacggcatc gccgccgcat tgcaggcgat tgccgattcg
      781 ggtttggacg atgtggaaaa cctcgacaaa gaccgcatcg gcgtgaacat cggttccggc
      841 atcggcggac tgcccagcat cgaggccacc ggcaaagccg taatcgaagg cggcgcgcgc
      901 aaaatcaacc ctttctttat ccccggttcg ctgatcaacc tgatttccgg acacgttacc
      961 atcctcaaag gctaccgcgg cccgagctac ggcatggttt ccgcctgtac caccggcgcg
     1021 cacgccatcg gcgattccgc ccgactgatc aaatacggcg acgcggacat aatggttgcc
     1081 ggcggcgcgg aaggcgcaat cagcaccttg ggcgtgggcg gttttgctgc gatgaaagcc
     1141 ctctccaccc gcaacgacga ccccgccacc gcttcccgtc cgtgggacaa aggccgcgac
     1201 ggcttcgtca tcggcgaagg cgcgggcata ttggtgttgg aagaattaga acacgccaaa
     1261 aaacgcggcg cgaaaatcta cgccgaaatc gtcggcttcg gcatgagttc cgatgcttac
     1321 catatcaccg cgccgaacga agaaggcccc gcccttgccg ttacccgcgc gctgaaagat
     1381 gccggcatca atcccgaaga cgtggattac gtcaacgcgc acggcacgtc cacccccttg
     1441 ggcgatgcca acgaaaccaa agccctcaaa cgcgcgttcg gcgaacacgc ctgcaaaacc
     1501 gtcatcagct cgaccaaatc catgaccggc cacctgctcg gcgcggcggg cggcgtggag
     1561 gccgtgtaca gcattttggc gatacacgac ggcaaaatcc cgccgaccat caacattttt
     1621 gaacaagacg ttgaagccgg ctgcgatttg gactactgcg ccaacgaagc gcgcgacgcg
     1681 gaaatcgacg ttgccatttc caactccttc ggcttcggcg gcaccaacgg tacgctggtc
     1741 ttcaaacgct tcaaaggctg attccgcaaa gccgccgccg acatcgaaat gccgtctgaa
     1801 accgtttcag acggcatttt tatagtggat taacaaaaat caggacaagg cggcgggccg
     1861 cagacggtac aaatggtacg gaaccggttc gcccggtgct tcagcacctt agggaatcgt
     1921 tctctttgag ctaaggcggg gcaacgccgt accggtttaa agttaatcca ctatactaac
     1981 tggaattaac aaatacggca caaatattaa cccgaaccgc aaacgcccat caagcccttt
     2041 cagacggcat cggcagacat accgttggct atatccaaag aatcaggaat tatttagccc
     2101 gaatcatccg gtttgttaaa attcgcaaac ttaaccgact caaaatacat attatgacgc
     2161 acaccgcatc gaaaacaccc aaactctggg cggtcattgc cgccgccgca ttcatcctgc
     2221 tgattaccat cggcatgagg atgacgctcg gactgttcgt ccaacccgtc gtcaacacca
     2281 ccgaattgaa cattgcccag ttcagcctca tcatcaccgt tttccaactg atgtggggcg
     2341 tattgcaacc attgtccggc gcgcttgccg accgtttcgg cgcgttcagg gtattaagcg
     2401 gcggtgccct cctgctcgtc tgcgcctgcc tgattgcctc caacatcccg acttattggg
     2461 ggctgatgat tgccgtcggg ctgctgctcg cattcggcac cggttccggc gggttttcca
     2521 tcattatggg acaggtcgcc gcccaagtcc ccacacacaa acgcggcttg gcatccggac
     2581 tggtcaacgc aggcggttcg gcaggacaat tcctgttcgc accgctggtt caaggactca
     2641 aggactcgtc gtcctgcccg aagtcggctg gacgggtaca ttttacgttt ggggcgcaat
     2701 cgccctgctg attctgcccg tctcatggtg gcttgccggc ggcaacaacg ccgcccacac
     2761 ccaacacacc caagccacac acggacaaag cctcggagaa gcagtcaaaa ccgccttcaa
     2821 aaccccaagc tacatcctgc tgcacctgag ctttttcgcc tacggcttcc acatcgcctt
     2881 tctcgtaacc cacctaccca cggaagtcgc cctgtgcgga ctgcccgcca ccgtcgcctc
     2941 gacatccatc gccatcatcg gactggcaaa catcgccggt tgcgtgttct caggctgatg
     3001 caccggacgc ttcaaaggaa aacacatcct gttcggactc tacgcctcac gcgtcgccat
     3061 ggtgctgatt tacatcttct cacccaaaac cgacctcaac ttttacattt tcgccgccgc
     3121 actcggattc acatggctcg ccaccgtcgc cccgaccgcc gccgttaccg gcaaactctt
     3181 cggcacgcgc tacctcgcca ccctgttcgg actggtgatg ctcacccacc aaatcggcgg
     3241 attcctcggc tcgtacatcg gcggcatcgt gattacacaa ttcggtgact acggctggat
     3301 gtggtacgcc gatgccgtac tcgccggcac ggcagccctg cttgtcctgc ccgtccgcga
     3361 accccgaacc gccgcatgac agaatgccgt ctgaaacctt cagacggcat tttccaaaca
     3421 ccccgcccgt tgccggactg cacggcacgc aaatcagcct tatcaaaccg caaataaccg
     3481 ccgcgattaa accgccattc aaataagccc gttttccctt cgggtgtaaa atcatcacaa
     3541 aacgcgatac tgtatatcgc tgggcgttat gcagcggatt tcaggataaa atgccgtctg
     3601 aagccgcccc tgcccgggcg ggcgaagcgt cggatgcggc gcgcttggca aaggtttgcc
     3661 cgccgcgccc cgtatccggc atttcatcaa tgcagaccgc atttccaatc ctatattcaa
     3721 ggtgtccgcc ttatgaaaat cgtttttatc acaacagtcg catccagcat ttacggtttc
     3781 cgcgcccccg tcattaaaaa attaatcggc aaaaaccatc aggtgtatgc ctttgtatcg
     3841 gagttttccg ataatgagtt ggacattatc agggaaatgg gggttacacc cgttacctac
     3901 cggtcaaacc gcagcggggt aaacccgttt tccgatataa aatccacctt cctcatattt
     3961 aaagcactca aaaaaatatc gccggatttg gttttccctt atttcgcaaa acccgtgatt
     4021 ttcggcactt ttgccgcaaa attggcaggc gtgcccagaa tcgtcgggat gctggaaggt
     4081 ttgggattcg catttacccc gcagccggaa ggcataccgt taaaaacaaa aataataaag
     4141 ggcattttga ttgccctgta ccgcattgcc ctgccgatgt tggaaagcct gatcgtatta
     4201 aaccccgacg acaaagacga gctgctgcat caatacggca tcaaaataaa aaacattcat
     4261 attttgggcg gaatcggtct ggatttgcgg caatatcctt attccgaggc ggatattccc
     4321 gatgaaaaag aacccgtaaa atttctcttt atcggcagat ttctgaaaga aaaagggatt
     4381 gatgatttta ttcgggcggc ggaacaggtt aagggcaaat accccgatac ggtttttacc
     4441 gctttgggcg caatcgacaa accacgcggg agcggggatt tggaacagcc tgccgcccgc
     4501 gatattatcc gtttccccgg ttttgtgaac aatgtttccg aaatgataaa
//
LOCUS       DAXFHF010000096         4528 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXFHF010000096 DAXFHF010000000
VERSION     DAXFHF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.096"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..215
                     /locus_tag="ACFIRM_001946"
     CDS             <1..215
                     /locus_tag="ACFIRM_001946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029497.1"
                     /translation="HRACCAFLCVWRVRRREICLLFPRRAGVTGGTLSINRNTVYNDR
                     LFHHIPERNNVSTHGTAHQAPPYVSS"
     gene            313..1233
                     /gene="ribF"
                     /locus_tag="ACFIRM_001947"
     CDS             313..1233
                     /gene="ribF"
                     /locus_tag="ACFIRM_001947"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC3029498.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLELLEGTGCVDAAWVLRFDRHF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTTVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVGGGRFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1375..4164
                     /gene="ileS"
                     /locus_tag="ACFIRM_001948"
     CDS             1375..4164
                     /gene="ileS"
                     /locus_tag="ACFIRM_001948"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC3029499.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1078 a         1280 c         1234 g          936 t
ORIGIN      
        1 agcaccgcgc ctgctgcgcg tttttgtgcg tttggcgcgt tcggcggcgg gaaatttgcc
       61 tacttttccc gcgtcgggcg ggcgtaacgg gcggcacact gtctataaac cgcaataccg
      121 tttacaatga ccgcctgttt caccacatac ccgaacgcaa caatgtttca acacacggga
      181 cggcacatca agcaccgccc tatgtgtcgt cctgatttgg aaggggttgc acccctcccg
      241 aataaagtct gatcctgccg ccccgaagga cggatgtccg agtggcgggg tttcaaccga
      301 aaaggaaata caatgaaaat caggccgggg cggcacaacg cgcccgactt tccgcacggg
      361 gccgccgtaa ccataggcaa tttcgacggc gtgcacctcg gacacaaaca catcctccaa
      421 aaactccgcc tcgaagccga cgcacgcgga ttgcccgtcg ttgccgtcat tttcgagccg
      481 caacccaaag aattttttgc cgcccgcaca ggcaaaaccc cgccctgccg tatcagcccc
      541 ctgcgtacca aactggaatt gctggaaggg acgggttgcg tcgatgccgc ctgggttttg
      601 cgtttcgacc ggcatttttc cgaaatatcc gcgcaagcat ttatcgaccg cctgctgcgt
      661 caaactttga atacgcgcta tttgctcgtc ggcgatgatt tccgtttcgg cgcggggcgc
      721 gaaggctgtt ttgaactttt ggcacaacag cccgatatgc aaaccgagcg cacgccttcc
      781 gtcatcgtcg aagacatccg caccagcagc accaccgtcc gccaagccct ttcagacggc
      841 aaccttgcct atgcgaaaaa acttttggga cacgactacg ttttgggggg cagggtggtg
      901 cacggcagaa aactcgggcg caccttaaac gccccgaccg ccaatatccg gctgcccggc
      961 caccgttatg ccctcggcgg cgtgtttgtc gtcgaagcgg acggcgcatt cggcacgcgg
     1021 cgcggcgtgg cgagcttcgg cttcaatccg accgtcggcg gcgggcgttt tcaaaagctg
     1081 gaagtccatt tgttcgactt tcaaggcgat ttgtacggac aacggttgaa cgtccgcttc
     1141 ctgcacaaac tgcgcgacga ggaaaagttt gacggtatgg aagaactgaa aaggcggatt
     1201 gaagccgata tggaagccgc aaagtgttgg tagaaaaacc ttatacaaac catccgattg
     1261 ggctacaatc agccttttaa ctgttcagac ggcatagggg tttcccgttg tgaaatactg
     1321 tttgaggcgc aatgccgtct gaaactgaaa tattgtaaca atagagatta aaaaatgacc
     1381 gattacagta aaaccgtcaa cctgctcgaa agcccgtttc cgatgcgcgg caatcttgcc
     1441 aagcgcgagc ctgcgtggct gaaaagctgg tacgagcaaa aacgttacca aaaactgcgc
     1501 gaaatcgcca aaggccgtcc gaaattcatc ctgcacgacg gcccgccgta tgccaacggc
     1561 gacatccata tcggtcatgc cgtcaataaa attcttaaag acattattat ccgcagcaaa
     1621 acccaagccg gttttgacgc gccttatgta ccgggttggg actgccacgg tttgcctatc
     1681 gaagtgatgg tggaaaaact gcacggcaaa gatatgccta aagcccgttt ccgcgaattg
     1741 tgccgcgaat atgccgccga acagattgcc cgtcagaaaa aagactttat ccgcttgggc
     1801 gtgttgggcg actgggacaa tccttacttg accatggatt tcaaaaccga agccgatacc
     1861 gtgcgtatgc tcggcgaaat ttacaaatcc ggctatctct accgtggtgc gaaaccggtt
     1921 cagttttgct tggattgcgg ctcttcgctg gcggaagcgg aagtggaata caaagacaaa
     1981 gtatcgcctg cgattgacgt tgcctatccg tttaaagaca ccgtcgcgct tgccgccgca
     2041 ttcggcttgg caggtatcga aggcaaagcg tttgccgtca tttggacgac cacgccttgg
     2101 actctgcctg cgagccaggc cgtgtctgcc ggcgcggacg tggtgtatca attaatcgat
     2161 acgcccaaag gcaaattggt gctggcgaaa gatttggcgg aaggcgcttt gaaacgctac
     2221 ggcttttcag acggcatcgc catccttgcc gaaaccaccg gcgacaagct ggaaaacctg
     2281 cacatgaatc atccgttcct cgaacgcgat attcccatgc tcaacggcga acacgttacc
     2341 accgatgccg gtaccggctt ggtgcatact gcgcctgcgc acggtttgga agactacgcc
     2401 gtctgcaata aatacggcat cgagctttac aaccctgtca acgccgaagg caaatacata
     2461 agcgaaacgc ctcgtgtcgc aggcatgagc gtttgggagg cgaatcccgt catcctgcaa
     2521 tggccggaag aaaccggcaa cctcttggca agcagcaaaa tcgaacacag ctacgcccac
     2581 tgctggcgcc acaaaacccc gctgatttac cgagcgacag gtcagtggtt tgtcggcatg
     2641 gacaaagccg gcagcgacgg taaaaccctg cgcgacaaag ccatcaaagc cgtggacgac
     2701 accgaattct tcccgccatg gggtcgtgcg cgtttggaat ccatgattga aggccgtcct
     2761 gactgggtgg tttcacgcca acgctattgg ggcacgccga tgactttctt tgttcacaaa
     2821 gaaaccggtg aattgcatcc gaactctgcc gaacttttgg aaaaagtcgc gcaacgcatc
     2881 gaagaaaaag gcatcgaggc ttggttctcc ctcgataaaa gcgaattatt aagcgcggaa
     2941 gattgcgaac attacgacaa actccccgat accatggacg tatggttcga ctcaggctcg
     3001 acgcattatt ccgttgtaaa acaacgcgaa gaattggaat ggccggctga cttgtacctc
     3061 gaaggcagcg accaacaccg cggctggttc caatcctcta tgctgaccgg ttgcgcctca
     3121 tccatgggac gcgcaccgta taaacagctg ctgacccacg gtttcgtggt tgaccaaaac
     3181 ggccgcaaaa tgtcgaaatc catcggcaac gtcgtcgcgc cgcaggaagt ctataacgaa
     3241 ttcggcgcgg acatcctgcg cctgtgggcg gcatccaccg attacagcgg cgaattggcg
     3301 atttccaaag aaatcctcaa acgcgttacc gaaagctacc gccgcatccg caataccttg
     3361 agcttcctgt ttgccaattt gagcgacttt aatccgattg aagatgccgt gcaacaggcg
     3421 gatatggtgg aaatcgaccg ctacgccttg gtattggcgc ggcggctgca agagcgtttg
     3481 gcaggcggtt actatccgcg ctatgccttc cacttcgccg tgaaagacat tgtttctttc
     3541 tgctcggaag acttgggcgc gttctacctc gacatcctga aagaccgcct ctacaccacc
     3601 aaagccgaca gccgcgcccg ccgcagcgcg caaaccgccc tgtaccacat cacgcgcagc
     3661 ctggttctct tgattgcacc gattttgtgc ttcaccggcg aagaagcgtg ggacatcatc
     3721 ggcggcggcg aagaagacag cgtcctcttc catacttggc acgagttccc ggccatcaac
     3781 gaaaaagccg aagccgaact ggtgaaaaaa tggacggcaa tccgcgaagc gcgcgaagca
     3841 gtaaccgccg ccatcgagcc tttgcgcgcc gacaaaaccg tcggttcgtc cttgcaggcg
     3901 gaagccgaaa tcaccgcgcc ggaagaaatg gccggctatc tgaatgcttt gggcgaagaa
     3961 ttgcgctttg ccctgctggt gtctaaagca gaagtgaaag tcggtgatga acttgccgtt
     4021 gccgccaaag ccagcgacgg cgaaaaatgc gaacgctgct ggcactacac ccgcgatgtg
     4081 ggcgcggttg caggctatga aaccgtctgc aaacgctgtg cggagaatgt cggcggagaa
     4141 ggcgaaacgc gccattacgc ctgataaagt ttgagcaaat gccgtctgaa accgccaaac
     4201 agtatttcag acggcatttt ttgtgccgcg atttgtcttc ataatggcgg acgggtttcg
     4261 agattgcggt attgtcggga atgacggttc gggtatttta ctgcgcccgc cccgcgcctg
     4321 taaacggcgg gcgatcaaaa atgccgtccg aaggttcaga cggcatcggt gtcggggaat
     4381 cagcgggccg tagcaaccga accgtcattc ccgcgaaagt gggaatctag gatctcgggg
     4441 tttcagtcat ttccgataga ttcccgccgc gtcaggggtc cggattcccg cctgcgcggg
     4501 aatgacgggt ttcgagattg cggtgttg
//
LOCUS       DAXFHF010000097         4490 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXFHF010000097 DAXFHF010000000
VERSION     DAXFHF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.097"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(171..371)
                     /locus_tag="ACFIRM_001949"
     CDS             complement(171..371)
                     /locus_tag="ACFIRM_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029500.1"
                     /translation="MNELISKINRVGAREKDGQSLLLKVGEICRDAGATFTTRKSESL
                     NHTAFTFTVKKDGLKDKAMIVL"
     gene            complement(612..1028)
                     /locus_tag="ACFIRM_001950"
     CDS             complement(612..1028)
                     /locus_tag="ACFIRM_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029501.1"
                     /translation="MPQSWQCRQGACLLIPSGPNSCQHLFTILVNPCILPNRGNKPQV
                     LSVGISSVRANIPYDNACIIRQGEHPFVRHDSYVRYRDARIDAVEHIENRVHEGIFSV
                     KPPCSEQLLKRIIAGASTSRYAGREVKLLIEKFAIA"
     gene            complement(1037..1621)
                     /locus_tag="ACFIRM_001951"
     CDS             complement(1037..1621)
                     /locus_tag="ACFIRM_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC3029502.1"
                     /translation="MKPHNDTIFQEPKAAQAAAFLLYKANGRLEVLKLMKLMYLAERE
                     SFLRFGEGLTGDALVSMPHGPVLSMTLDFINGGHESVPNGWATWVSDRENRMLALRDP
                     SMIRTPGQDLSALSEADLEVLESVWENYGHYSAWDLRNMTHNGLCPEWEDPHGSSRPI
                     PIKKLLSVLGYDDEQAVAIVERLEEQAYINRAFG"
     gene            complement(1803..2204)
                     /locus_tag="ACFIRM_001952"
     CDS             complement(1803..2204)
                     /locus_tag="ACFIRM_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3029503.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(2306..2488)
                     /locus_tag="ACFIRM_001953"
     CDS             complement(2306..2488)
                     /locus_tag="ACFIRM_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC3029504.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(2658..3476)
                     /locus_tag="ACFIRM_001954"
     CDS             complement(2658..3476)
                     /locus_tag="ACFIRM_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC3029505.1"
                     /translation="MNRYAMRFAVIRFMPYVQTREFANIGIIITHPQSGCFDFKIEHR
                     YSRLSRFFRRFDPPAYKAATRAFEKELQRIRNLAAHSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKNQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGRKDPADIYYKSDKRIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVSGDLEEQGIQLADNRSEGKIIRNFACG"
     gene            complement(3749..>4490)
                     /locus_tag="ACFIRM_001955"
     CDS             complement(3749..>4490)
                     /locus_tag="ACFIRM_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC3029506.1"
                     /translation="LNEIETAECAELKRIFNSKKEELKLTQYKLAEAVGVTQSAVNHY
                     LNGTNALNASIASQFAKILQIPVSDFSLRLAEEISSMSIGIDGDKLLALQADNLDTNT
                     ITLNLYDVSASCGHGVVNPDYPQLLRSIEIPNDALFELLGTNNLTNVQLMPPDGDSME
                     PTIPQKSITLIKTDVSKFQTGGIYLFTFDGYTYIKRLSRGKGGAIHATSDNRHYAKSD
                     FLINPEEADKFHIHGKFWKVLPLDFLDL"
BASE COUNT          968 a         1041 c         1176 g         1305 t
ORIGIN      
        1 ctgccgtaag ccgccgtgcc gtaatatttc accgctttat ccttaagttt gaggaaaccg
       61 cccgcagcat tcaccgtttc gccgtgccgt tgccccgctt tgaagttcga tacttcatcg
      121 ctttgtgcta tccccggctt ggcagatata gctttcgggc ggtttgttac ttacaaaaca
      181 atcattgcct tatcttttaa gccgtctttt ttaaccgtaa aagtaaaagc ggtgtgattg
      241 aggctttcgc tttttcgggt ggtaaaagtt gcccccgcgt cgcggcagat ttcgccgact
      301 ttcaataaaa ggctttgccc gtccttttcc ctcgcgccga cccggtttat tttgctgatt
      361 aattcgttca tcccgtttcc ttcaagttgt tgtttgtttc gatgggtgta tattaccggc
      421 agtaatttat tattgcaata ccgctagtaa tattatttac ggtaatttat atttatcttt
      481 gattattaaa agaatttatt tttgaaattc cgcaggcgca aaaaaaccgc ctattaaggc
      541 ggctttgtcg gttttgtgtt gttttcaggt tcggcgggcg tggaaaaagc ccgcatgatg
      601 cgggcggagg attatgctat agcaaatttt tcaatcagaa gcttaacttc cctgccggca
      661 tatcgtgatg ttgaagcccc ggcaatgata cgcttcaaca gttgttcgct acatggtggt
      721 tttacggaga atattccttc atgcacacgg ttttctatgt gttcgacggc atcaatcctt
      781 gcatcacggt agcggacata actgtcatgt cggacaaaag gatgttctcc ctgcctgatg
      841 atgcaggcat tatcatatgg gatatttgcc cggacggaag aaatgccgac agaaagaacc
      901 tgcggcttat ttccacggtt gggcaggata cacggattga ctaggatggt aaacaagtgt
      961 tggcagctat ttggaccgga tgggatgagc aggcatgcgc cttgcctgca ttgccacgat
     1021 tgaggcatac cgccccctac ccgaaagcgc gattgatgta agcctgttct tccagccttt
     1081 ctacaatcgc tacggcttgt tcgtcatcat aaccaagcac agacaacagt tttttgatgg
     1141 gaatcgggcg gcttgagccg tgagggtctt cccattccgg gcataacccg ttgtgggtca
     1201 tgttgcgcag atcccatgcg gaataatggc cgtaattctc ccaaaccgat tccagtactt
     1261 ccaaatcagc ttcggataat gccgataagt cttgcccggg cgtccgaatc atacttggat
     1321 cgcgtaaggc aagcatcctg ttttcgcggt cgctaaccca cgttgcccat ccgttcggaa
     1381 cggactcatg tccgccgtta atgaaatcca aggtcatgga gagtaccgga ccatgcggca
     1441 ttgaaaccaa cgcgtcacct gtcaggcctt ctccgaagcg taaaaaagac tcacgttcag
     1501 ccaagtacat cagcttcatc agcttcagta cttcaagcct gccgtttgcc ttgtacagca
     1561 ggaaggcggc cgcttgggca gcctttggct cttggaaaat ggtatcgttg tgcggtttca
     1621 tacttttgat tatatgcggt ttcctgtaaa gcaggcaatc taaatccggc acatttccgt
     1681 acattttaca tcttttcgat atatttcagc atattcctca atccggcacg aaccgtttgt
     1741 gttgttttca ggttcggcgg gcgtggaaaa gcccgcgtgt gcgggcgggt tgaacgggct
     1801 ggctatgcct gattcatggt gagcagggcg gctttggcta aaaaaccgct acgggtttca
     1861 tgatttgcgg atgtatattc atccactcta tcaagaaggt attgcggcca gctgacgttg
     1921 aatcggattt gctgccgact gatttttgca gggtcgattt caatcattac ccatgttgcc
     1981 ccatgataat caggttcttg gctcaaatcg gcaatgctgc ttacggcaag gtttttgaac
     2041 cctccgtcct caatcatacc gtcaatatgc atataggcgg cagaacgggc gttggcgacg
     2101 gcttcttcaa cggtgtcgcc gcaagagaaa cagccgggaa ggtcgggaat ggttacgcca
     2161 tatgccgaat gttcgtcttt gtgcagggcg gcagggataa acatttgatt tcctttaatt
     2221 atgttgttca ggtttatggg cgcgtgagat aacccgttga ttgtttgatt agaagggggg
     2281 cagcgggaaa ccccgctgcc tgccgttact tcaaaccggc ttgcttatag atatttttta
     2341 cagtacctgt cggcaagtct tttttcgggt gcggcacggt tacacggcct ttttttgttg
     2401 ggtgcttata ttgcgaatgg ctcccggatt gtgcaacttt ataccaaccg tcttgtttga
     2461 gtagggcaat aacgtctagg ctattcaatt atcactccta atcttaatgc atggcacgcc
     2521 tgccaatgtg cgtaataata cacactaatt tttattttgt caatttttat gtgtatttgt
     2581 gtgtaaaaat atctttcggc agtagttgca gggaagggtg taaaaagccc gcataacgcg
     2641 ggcggtcttg agggctgtca gccgcaggca aagtttctga tgattttgcc ttcgcttcgg
     2701 ttatcggcaa gctgtatgcc ctgttcctcc aggtcgcccg atacgtcaag caatgccttt
     2761 tcttgtgcct tgtcgggacg ctccggcggt tcgtaggcaa acaatatttc agagcggtcg
     2821 atgtatccgc cgcgccgcaa ccgttttatg ccggcaatcc gcttatcaga cttgtaatag
     2881 atgtcggcag ggtctttccg tccgaaatag ataggcttga tgattttccg tatttcgccg
     2941 ttttccgcct tttgcaccaa ggggatggag gcgtgaaagc ctgacgggtc gccgattgtg
     3001 ctttctttga aggggtatgc cgtctgaagc ggctttaaca ttgcctgtat ttgtcgggta
     3061 agttctgctt cgggttggtt tttggcgaag ctgcgggcga caaaataatc aaacaggcgg
     3121 ttcagttcct gccccctgtc gggggcgagg gttacgcccg gttgggcggc cataatcagg
     3181 gcttcgcgcg ggcgggtcaa atggtcgagc atggcgcgta tctgatcggg cgcggagtgt
     3241 gccgccaggt ttctaatccg ctgtaattct ttttcaaagg cacgggtcgc cgctttatag
     3301 gcgggcggat cgaagcggcg gaaaaaacgg ctcaatctgc tgtagcgatg ttcgatttta
     3361 aagtcgaagc agccgctttg cgggtgggtt atgatgatgc cgatgttggc aaattcgcgc
     3421 gtttggacat agggcataaa ccgtatgacg gcaaaacgca tggcatatcg gttcatgaga
     3481 tgctccaaag tgttccgtta ccgatgcggc ggacggcctc gtcggtgcgg ctgcggttat
     3541 aggcggcggg caggtcgcgc tcttcgtttg cccgtgcccg ctccggcggc agattatcac
     3601 acgcttttct tttctttacc ctatgaaatt tacactttgt gtaaatctgt aaatccgact
     3661 ttttgagatt tcccgggagg aatttgtcaa tccggcacgc cctgccggaa gaccgggccg
     3721 atgacggtca ggctgtccaa aggggcggtc aaaggtctaa aaaatcaagc ggcaaaactt
     3781 tccaaaattt gccgtgaatg tggaatttgt cggcttcttc agggtttatc agaaaatccg
     3841 acttggcgta atgcctgttg tcgctggtag cgtgtatcgc gccgcctttg cctctggaca
     3901 ggcgtttgat gtaggtataa ccgtcaaagg taaacaggta gatgccgccg gtctgaaact
     3961 tgctaacgtc tgttttgatt aaagttatcg atttctgtgg aatggtcggc tccatgctgt
     4021 cgccgtccgg cggcataagc tgcacgtttg tcagattgtt tgtccccaaa agctcgaata
     4081 gcgcgtcatt gggaatctca atcgagcgta gcagttgcgg atagtcgggg ttgactaccc
     4141 catgaccgca actggctgat acgtcgtata ggttgagggt gatggtgttg gtgtcgagat
     4201 tgtctgcttg cagtgccagc aatttatctc catcaatgcc gatggacata ctgcttattt
     4261 cttcggcaag tcggaggctg aaatccgaaa caggaatttg caagattttg gcaaactggc
     4321 ttgcaatgga ggcgtttaag gcgttggtgc catttaaata atggttcacc gcgctttgag
     4381 taactcctac agcttctgcc agcttgtatt gggttagctt aagttcttct ttttttgagt
     4441 taaaaattct ttttagttcg gcacattcag ccgtctcaat ttcatttaat
//
LOCUS       DAXFHF010000098         4181 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXFHF010000098 DAXFHF010000000
VERSION     DAXFHF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.098"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            144..1224
                     /gene="hupA"
                     /locus_tag="ACFIRM_001956"
                     /pseudo
     CDS             144..1224
                     /gene="hupA"
                     /locus_tag="ACFIRM_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            1252..3681
                     /gene="hupB"
                     /locus_tag="ACFIRM_001957"
     CDS             1252..3681
                     /gene="hupB"
                     /locus_tag="ACFIRM_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC3029507.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLANDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1133 a         1298 c          967 g          783 t
ORIGIN      
        1 ctataaaaaa caaaggtaga aacgcggcag acaaacgcag gcacgtccga tttccgcgcc
       61 gccccataaa acagttgaca tcatataaca tttaaattga taatcaaaac tattattaat
      121 acatcatcaa gattgagtat gcgatgaaat acaaagccct gcccttactg ccgcttgccg
      181 ccgcccttgc cgcctgtgcc ggggggggta gccgaaccgc acgtccccgt gtccatcccc
      241 accgccacgc cgctgcccgc cggcgaggta acgttatcaa gcgataacgg caatatcgaa
      301 aacatcaaca ccgccggcgc cggaagcgca tccgacgcgc cgagccgcag cagacgctcg
      361 ctcgatgccg ccccgcaaaa cacatccggc atttccattc ggcagcggga agtagaaaaa
      421 gactatttcg gctataaatc aaaagaaaca tcattcatct tcaaaacacc cggcggcgcg
      481 caatacgccc tttcatccta cgccgacccc ataaccgtca gctactcctc ccccgatttc
      541 aaaattcccg accgccacgc agggcagcgg ctcgccgacg gcagccgcat ctttatctgc
      601 tgcagcgact ccggggctac ttcgtatgcg gaaattacca aacaagatta tatgaaattc
      661 ggggcgtgga taggtcccaa cggcgaaatc gacctcttcg ccggcggctt ccccgtcggc
      721 aaaacgccgc ctcctgcgtt ctcctacggc agcagcacgc ccgaaaccgc attgtcaaag
      781 ggtaaaatca cttatcaggt ttggggcatc cgcgtcagaa acgggcaatt tgttacttcc
      841 tcttatacgc cgccgaaaag cggttcttat tatgggacac ttgccaatac ccccgtcctt
      901 tcctttatca ccgccaattt caacagcaac acactggcag gcaaaatcct cggcaacagc
      961 gattacgggc cggatgtgga tattcaaaac gcaacgatta ccggtccgac cttttccggc
     1021 gatgccacgt ccggcgggaa aagcggcaaa ttggaaggca agttttttgg caagttcgcc
     1081 agcacccgca gcagcgaagt cagcataggc ggcaaaatca cctttgacgg cgaccgctcc
     1141 ctcgataccg tattcggcgg tgtgagttat gaaaaaaaac ttgatgacac cagtcaagat
     1201 accaaccacc ttactaaaca ataaaccacc tcatccctgc aaaggtttct tatgcccatt
     1261 cccttcaaac ccgtattggc tgccgccgcc atcgcccaag cgtttcccgc ctttgcggca
     1321 gaccccgcgc cgcagtccgc ccaaacgctg aacgaaatca ccgttaccgg cacgcacaaa
     1381 acccaaaaac tcggcgaaga aaaaatccgc cgcaaaacct tagacaagct cttggccaac
     1441 gacgaacacg acctggtgcg ctacgacccc ggcatttccg tcgtcgaagg cggcagggcg
     1501 ggttctaacg gctttaccat acgcggcgtg gacaaagacc gcgtcgccat caacgttgac
     1561 gggctggcgc aggcggaaag ccgctcttcc gaagccttcc aagaattgtt cggcgcgtac
     1621 ggcaacttca acgccaaccg caacacttcc gagccggaaa acttttccga agtaaccatc
     1681 accaaaggcg cggactcgct caaatccggc agcggcgcat tgggcggcgc agtcaattac
     1741 caaaccaaat ccgcaagcga ctatgtttcc gaagacaagc cctaccattt ggggataaag
     1801 ggcggcagcg tcggcaaaaa cagccaaaaa ttcagcagca ttaccgccgc cggcaggctc
     1861 tttggtttgg atgccttatt ggtttatacc cgccgcttcg gcaaagaaac caaaaaccgc
     1921 tcgaccgagg gcgatgtcga aattaaaaac gacggatatg tctttgaccc ggccaacccc
     1981 tcgcccagca gataccttac ctacaaagcc acaggggttg ctcgctccca acccgacccg
     2041 caagaatggg taaacaaaag caccctgttc aagctgggct acaatttcaa cgaccgcaac
     2101 cgcatcggct ggatttttga agactcgcgc accgaccgtt ttaccaacga gctgtctaat
     2161 ttgtggacgg gtacgaccac gtctgccgca acgggcgact accgccaccg ccaagacgtg
     2221 agctaccgcc gccgcaccgg cgtcgaatac aaaaacgaat tggaacacgg cccgtgggac
     2281 agcctcaagc tgcgctacga caagcagcgc atcgatatga acacttggac ttgggacatc
     2341 ccgaaaaatt acgatacaaa cggcatcaac ggcgaggttt accattcgtt ccggcatatc
     2401 cgccaaaaca ccgcgcaatg gactgccgat tttgaaaaac aactcgactt ttccaaagcc
     2461 gtttgggcgg cgcaatacgg cttaggcggc ggcagagggg acaatgccaa ctcggattac
     2521 agttatttcg caaaactgta cgaccccaaa atcctcgctt ccaaccaagc caaaatcacg
     2581 atgctgatcg aaaaccggtc gaaatataaa tttgcctatt ggaacaatgt gtttcacttg
     2641 ggcggcaacg accgcttccg cctgaatgcg ggcatacgct acgacaaaaa cagcagcagc
     2701 gcgaaagacg atccgaaata caccaccgcc atccgggggc agattcccca tttgggttcg
     2761 gaacgcgcgc acgcgggctt cagctacggc acagggttcg actggcggtt taccaagcat
     2821 ctgcacttgt tggcaaaata cagcaccggc ttccgcgcac cgacttcgga cgaaacctgg
     2881 ctgctgttcc cacaccccga tttctacctg aaaaccaatc ccgaactgaa agcggaaaaa
     2941 gccaaaaact gggaattggg cctggcgggc agcggcaaag ctggcagctt caagctttcg
     3001 ggcttcaaaa ccaaataccg cgactttatc gaattgacgt atatgggcgt ttcgtcagac
     3061 gataaaaaca accccagata cgccccgctt tcagacggca cggcattggt cagctcgccc
     3121 gtttggcaaa accaaaaccg caccgccgcc tgggtgaaag gcatagagtt taacggcact
     3181 tggaacctcg acagcatcgg gctgcccaaa gggctgcaca ccggcctcaa cgtcagctac
     3241 atcaagggca aggcaacgca aaacaacggc aaagaaacgc ccatcaacgc gctttcgccg
     3301 tggacggcgg tttacagcct gggctatgac gcgccttcca aacgctgggg cgtcaacgcc
     3361 tacgccgcgc gcaccgccgc caaaaagccg tccgacaccg tccacagcaa cgacgacttg
     3421 aacaaaccgt ggccttatgc caaacacagc aaggcctata cgctgttcga cctttccgcc
     3481 tacctcaaca tcggcaaaca ggttacgctc cgcgccgccg cgtacaacat taccaacaag
     3541 cagtactaca cttgggaatc tttacgcagc gtccgcgagt tcggcacggt caaccgcgtc
     3601 aacaacaaaa cccacgccgg catccaacgc tttacctcgc cgggcaggag ctacaatttc
     3661 accatcgaag cgaagttcta agctcctgat gtgccaatgc cgtctgaaac ccgatgattt
     3721 gggtttcaga cggcattttt tacggtcgca ccgccgcccc ctttcaggct tgtaccaagc
     3781 ccaatcccgc aactctccgt cattcccgca actctccgtc attcccaggg aagtgggaat
     3841 ctagaaacgc aaagccacag gaacttatcc gaaacaacag caacctaaaa ttcgtcattc
     3901 ccacgaaagt gggaatccag ttcgttgagt ttcagttatt taaaataaat tttgaaactt
     3961 taatcccatc attcccgcga aagcgggaat ccggaacgta aaatctaaag aaaccgtttt
     4021 atccgataag tttccgtacc gacggggctg gattcccgcc tgcgcgggaa tgacggcgga
     4081 gcggtttctg ttttttccga taaattccta aaacttaaaa tttcatcatt cccacaagga
     4141 cagaaaacca aaaacagaaa cctaaaattc gtcattcccg c
//
LOCUS       DAXFHF010000099         4143 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXFHF010000099 DAXFHF010000000
VERSION     DAXFHF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.099"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..228
                     /locus_tag="ACFIRM_001958"
     CDS             <1..228
                     /locus_tag="ACFIRM_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFC3029508.1"
                     /translation="KKTVKFQFPPEVVRVLAGMGNNLNQIAKALNTAAKVGTLGNVEA
                     LKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            225..902
                     /locus_tag="ACFIRM_001959"
                     /pseudo
     CDS             225..902
                     /locus_tag="ACFIRM_001959"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            916..1503
                     /locus_tag="ACFIRM_001960"
     CDS             916..1503
                     /locus_tag="ACFIRM_001960"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFC3029509.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMMKALTHLGERLAELEQE
                     NTSLAQQLASLAAELERQSEIQQRQSEILNQLAKR"
     gene            1550..1804
                     /locus_tag="ACFIRM_001961"
     CDS             1550..1804
                     /locus_tag="ACFIRM_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HFC3029510.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1782..2060)
                     /locus_tag="ACFIRM_001962"
     CDS             complement(1782..2060)
                     /locus_tag="ACFIRM_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFC3029511.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2060..2245)
                     /locus_tag="ACFIRM_001963"
     CDS             complement(2060..2245)
                     /locus_tag="ACFIRM_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFC3029512.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2248..2493)
                     /locus_tag="ACFIRM_001964"
     CDS             complement(2248..2493)
                     /locus_tag="ACFIRM_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029513.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2490..2732)
                     /locus_tag="ACFIRM_001965"
     CDS             complement(2490..2732)
                     /locus_tag="ACFIRM_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFC3029514.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2736..3605)
                     /locus_tag="ACFIRM_001966"
     CDS             complement(2736..3605)
                     /locus_tag="ACFIRM_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC3029515.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
     gene            complement(3688..3912)
                     /locus_tag="ACFIRM_001967"
     CDS             complement(3688..3912)
                     /locus_tag="ACFIRM_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029516.1"
                     /translation="MKNSGKNTHAALSELVSCFPQNAALLAGLGRAAALQALPLRLLA
                     SLNCGLENFEIRTPIGLLSDYGYFDIVVSS"
BASE COUNT         1123 a         1024 c         1111 g          885 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag acggcttaga agccctagcc ctatcaggca
      781 agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt gaaatatcac
      841 gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc aacatccgac
      901 tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga gcagacatca
      961 cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac gttacggaaa
     1021 aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat aaacgcccgg
     1081 cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct aggtatatcg
     1141 gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt caagaacgag
     1201 ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct gttggaaacc
     1261 aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc aacgcgggta
     1321 tgcgtagcga ccgagaagcg aatgatgaaa gccttaacgc acttgggcga aaggttggca
     1381 gagctagagc aggaaaacac gagtttagcg cagcagctag cgagcttggc agccgagtta
     1441 gagcggcaga gcgaaataca gcaacggcag agcgaaatct tgaatcaact agccaaacga
     1501 taagccaacg acacaaacga acccaaagca ggggatgggg aatgagccga tgattaccga
     1561 gaacgaacgc gacaggcgaa cagccgcatg gctgatagag acctacgggg cagaagccgt
     1621 agcggaagca gaaacccgca ttgcgggcgc gagaaagccc tatccgagca atatcgccaa
     1681 agtattgggg gctagcctac ccgaagccct aaaacgcaca gaaaacgccg cagcgcgcca
     1741 aaaactggca gggctgcggc ggatgttgga cggtaaggca gttaagactt cacaagactt
     1801 gtgaaatcag accactgctc aatgcggaac gcccgaatat cgcggacaga agacggaaac
     1861 caaggcagag cttttagctc gttgatggct gaaaacaggt tagccatatc ttcgttttgg
     1921 caggtgtaca aacttccctg aatgcgggta aagccgaatt tccgcagggt gtatccaata
     1981 tccgcgtagg cttgggaaat gcctttcgga tggttttgag cggtatcggc aaccaccaaa
     2041 tcaaaagaaa tcgcgtacat taggcagcct ttgggtcttg aacgatgtgg gacagtttgt
     2101 attgcgtgtg ttcgccgctt tccggcagtt caatatttac gagccaatcg ccgtccggaa
     2161 gctgttcggc aggcgtgccg acgatgtaca tcacgccgta atcgccaaag gtgcggattg
     2221 aaccggtggg gatagtggtt tgcatggtta gttctcctgt tgggtttggt tttcaggctg
     2281 gtttgtcatg tacaaaaccc gttgggcagc tttttcggct gttgtgcgtt tgatgccgtc
     2341 aggcagaaaa attctccatt ttttggctag aactatatct gcaagatcat caaccgttgc
     2401 gcttttcaaa gcaggatttg cgttaatttc cgaatacaga aaatctttga atgccgagta
     2461 cagagcatcg cggtttattg cgtatcttgt catgctgttt cgctttggtt gtcagtttca
     2521 gacggcatag gcttgccgcg cctgtaccaa gtggcgcggg aaatgccgaa gtgttcccac
     2581 ggtttatcgc ggctgataga gttttcggct aggtagtcgg cgcgggattg cgaccatgcc
     2641 cgaaaaacgg ttcttgtgga acagccgaag cgttttgcca gttctttagc cgtaacgtct
     2701 cgcttggtgg ttttcagttt tggataggcc atatttcaaa gctccgtgat gatttttgtt
     2761 ctgcctgcat tgatggttgt tttccgcaaa ctattccgat gagcctgcaa ttccgaaaac
     2821 cgtcgattgc tggctgcaat atcaaacttg taccaaaccc agttagcgat tgatttggag
     2881 atacatttca cttctttttc ccacatcggg acagggaaat ccccatttac ccgcatacac
     2941 tggtaatgca cttctttcag ccagccttgc accgtgtagc cctgctgttt gaacgccaac
     3001 acgtttttgt gcgcccaacg gctcacaagg ttaaacaccg tgcaatttct gcttaatccg
     3061 accgcttcca cgttagagcg accgatatag ggcttaaact tgtctaaatc cacgaaatcc
     3121 gcaagatact ccaaatcgta gcccctgatt gcgtcaggaa cgccgcgcag cgtcagccaa
     3181 tgcggatgtt cgggattttt cgtaatcagc gatacaaagc ccacatcacc gcgcaatttc
     3241 gccttatatg ctgcttcaag tgcagcaaga tagcgcaggg ctttttgtct cccaccgtat
     3301 tccgccgtca gcacaggcgc ggaaagcgca taggcaaggt gtgcgccgcc gttttccctg
     3361 ttgattgccg cccaagcagg cataggcaga ttattgtctt cccaagccaa ccccgcccct
     3421 tcgtaatcca agtcaaagag cataaacaca cgcagatgcg gcggattgac ttggatgtag
     3481 cggcgtttga tggcggcagc gtaagagcgc accagcatag gcgcttcttt gaaatctttg
     3541 cagtatggct tgtgtgggat acgttcttgc aagaagaggt cgggttgggt gtataattgg
     3601 ctcatgttgt atctcgaaac ccccgtgcag attggcgttt ggcgggggtt ttgctttgtc
     3661 taagatttgc agattgtatg cttgttttta agatgataca actatgtcaa aataaccata
     3721 atcagataac agcccgatag gggttcttat ttcaaaattt tccaatccgc aatttagcga
     3781 agccagcagg cgaagcggta aagcttggag cgcagcagcg cgacctaagc cggccagcag
     3841 ggcggcgttt tgggggaaac atgaaaccag ttccgacagg gcggcgtgcg tgttcttccc
     3901 ggagttcttc atggagtatc ggcgaaatgc cgtgatgaaa tgccgttttt ttgagcagaa
     3961 agcagtcaaa aacaggggta ttttgccctt ttgacaggtt cgagtgccgc cgaaaagcga
     4021 acaaagcaac tcatcatccg agtcagcccg accgagtttg agactttgac ccgacagaag
     4081 acccatccga atttagcccg ctacattcgg gagcgggttt tggaagatgg caaagcatcc
     4141 gac
//
LOCUS       DAXFHF010000100         4006 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXFHF010000100 DAXFHF010000000
VERSION     DAXFHF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.100"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..214
                     /locus_tag="ACFIRM_001968"
     CDS             <1..214
                     /locus_tag="ACFIRM_001968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029517.1"
                     /translation="HNRGMPSETYIFILIQTAFRSDIGRTGANLRHAAQRRRLICRLK
                     AKMINCRLPFSDGILFSNEGVRRPQP"
     gene            225..1778
                     /locus_tag="ACFIRM_001969"
     CDS             225..1778
                     /locus_tag="ACFIRM_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC3029518.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAYIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFRRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIHKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1867..3894)
                     /locus_tag="ACFIRM_001970"
     CDS             complement(1867..3894)
                     /locus_tag="ACFIRM_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC3029519.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLRAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
BASE COUNT         1085 a         1156 c          971 g          794 t
ORIGIN      
        1 ccacaacagg gggatgccgt ctgaaacgta tatcttcata ttgattcaga cggcattcag
       61 gtcagacata ggaaggacgg gggcgaacct ccggcatgcg gcgcaaaggc ggcgtttgat
      121 atgccgtctg aaggcaaaga tgataaactg ccgccttccg ttttcagacg gcatattgtt
      181 ttcaaatgag ggcgttcgcc gtccgcaacc ataaaggaag tttcatgaac cggacttatg
      241 ccaatttcta cgaaatgctc gccgccgcct gccgcaaaaa cggcaacggc acggcagtgt
      301 tcgacggcaa ggaaaaaacc gcctaccgcg cgctcaagca ggaagccgaa gccgtcgcgg
      361 cgtatctgca aaatatcggc gtgaagttcg gcgacacggt cgcgctggcg gtttccaatt
      421 ccacagaatt tattaccgcc tatttcgccg tctccgccat cggcgcggtc gccgtaccga
      481 tgaacacatt tttgaaaaac agcgaatacg cgtatatcct gaatgactgc aaggcgcgct
      541 tcctgttcgc ctcggccggc ctgtcaaaag aattggcggg cctgaaggcg caaacgcccg
      601 tcgaaaaaat catttggacg gacaaaagcc ggccggccgg cgaaacggcg gaaggcgatg
      661 ccttttttga aaacgtgcgc cgcttccccg aaaaacccga cttggaccgc caaccccgga
      721 taaatgattt ggcatacatc atctacacct ccggcacgac ggggcatccc aaaggcgcgc
      781 taatcagtta cgccaacctg ttcgccaacc tgaacggcat cgaacgcatc tttaaaattt
      841 ccaaacgcga ccgctttatc gttttcctgc cgatgttcca cagcttcacg ctgacggcta
      901 tggtactgct gccgatttat atggcgtgtt cgattatttt ggtcaaatcc gttttcccct
      961 tttccaacgt tttgaaacag gccctgctca aacgcgcaac cgtgtttttg ggcgtacccg
     1021 cgatttacac cgcgatgagc aaggcgaaaa tcccttggta tttcagatgg ttcaaccgca
     1081 tccgcctgtt tatcagcggc ggcgcgcctt tggcggaaca aaccatcctc gattttaaag
     1141 ccaagttccg ccgcgccaaa ttgctggaag gctacggact gagcgaagcc tcgcccgtcg
     1201 tcgccgtcaa tacgcccgaa cggcaaaaag cccgcagcgt cggcatcccc ctgcccggtt
     1261 tggaagccaa agccgtcgat gaagaattgg tcgaagtgcc gcgcggcgaa gtgggcgaac
     1321 tgatcgtcag gggcggttcg gtgatgcggg gctacctcaa tatgcctgcc gccaccgatg
     1381 aaaccatcgt caacggctgg ttgaaaacgg gcgatttcgt taccatagac gaggacggct
     1441 ttatctttat cgtcgaccgc aaaaaagatt tgattatttc caaaggtcaa aacgtctatc
     1501 cgcgcgagat tgaagaagaa atccacaaac tcgatgccgt cgaagccgcc gccgtcatcg
     1561 gcgtgaaaga ccgttatgcc gacgaggaaa tcgtcgcctt cgtccaattg aaggaaggta
     1621 tggatttggg cgaggacgaa atccgccgcc acctgcgtac cgtgctggca aatttcaaaa
     1681 tccccaaaca gatccacttt aaagacgggc tgccgcgcaa cgctacgggc aaagtattga
     1741 aacgggtgct gaaggagcag tttgacggaa acaaatgaac gccgtgccgt ccgaagcccc
     1801 gtccggcaaa aaaatgcggt gaatctgatt caccgcattt tttggcaaac gccggaggga
     1861 aggcgcttac tctgccaatt ccacctgctc gtgcgccatc agttcctgac cgacatcatc
     1921 caacaacatc aaataacgtt cgtagttttt caaaatgtcg gcaatcagct cgtccttgtt
     1981 catatactgc acatcgtacc cgacgcgccc gtcgaaaaaa taagcgtagg gtttgtaagt
     2041 tgtctggtgc cggatatgcg gcagcttgcc gtcgttaatc aactggtcgg atacatcctg
     2101 cccgacagac ttaatcccgt acataaaatc gcgcatcgtc tctttccgaa tgacgaactc
     2161 gattgcgggc tcgtcctgat gaaacatctt atcgacccgg acgctcaagc cgtattcttc
     2221 cgaaagctcc cgttgcaact cgtgcatagc gggcgatgcg gtatgtttga ggaattttaa
     2281 aatatcctgc tcctgcgtct ggctcattat ccgcaccagc cgttctttcc acttgccgcc
     2341 cgtccaaaat acactggtag ggttgacccg ggtctcaaaa tatttcttat ccgcactcaa
     2401 gcctttccac aggctgaaac acattatcag catcagcagg gcaaacggca gggaaacaat
     2461 cagggtcata gactgcaggt tgccgagtcc gcccgagcgc atcagcaaaa cggcaacggc
     2521 agacatcagc acgccccaca taaccgcttg ccaccgtggc gccctcaagc ctttgtcccg
     2581 agaggtaata ttgttcagga cataaatccc ggagtcggca gaagttacaa aaaacaggga
     2641 aatgaccagc aggctgacga tgctcgtcag ttcgggcagg gggaggtaat taaagaattt
     2701 aaaaagcagc gtttccggag aggaggtcat cttttcgagc attccccccg caaccccgtc
     2761 attcagccaa atcgccgtat tgccgaagac ggtaaaccac aaaacgccga acaggccggg
     2821 gatgagcaaa accccgaaga caaactcgcg gatggtgcgc ccctttgaaa tgcgcgcgat
     2881 aaacaaaccc acaaacggcg cccaagaaca ccaccacgcc caataaagca ccgtccaaga
     2941 ttcaaaccac ggcttgtgtt cccgttcgta cgcataagtt ttcaaactga ggcgcaccag
     3001 atttccgagg tagttcccta tgttgtcgcc gaatgccgac aacaggtaaa cagtggggcc
     3061 cgccgccaaa acaaaaaaca gcagcaaaaa cgcaaggccc aggttcaact cgctcaacac
     3121 cttcacgccc ttccccacgc cggatattgc cgaaacgacg gcgagggaca ttacggcggc
     3181 gataatcaag acctgcacgc cgaagctgtt ttcggcaatc cagcccattt cctgcaatcc
     3241 ggcgcccagt tgcgaagccc cgaaccccaa tgtggtgatg atgccgaaaa aagtagcaag
     3301 caacgccata atatcaatgg catcgccgaa ccttccggaa attttttctt tcaacagggg
     3361 gtaaaaacaa gaacgcaggg caagcggcag tttgtagcgg aaaccgaaat aagccaaagc
     3421 caatgcaatc gtaccgtaca ccgaccaggc gtgaacgccc caatggaaca ccgtgtgcag
     3481 caatgcctgc tgctgcctgt gttccggcgc gccgaccgta atgtccgaaa aataatgcat
     3541 caacggctct gccacgccga aaaacatcag gcccacgccc atcccggccg caaacagcat
     3601 cgccagccac gacaggaagc cgaattccgg cacatcttca tcccgtccta gcctgatgtt
     3661 tcccaaactg ctgaccgaga gtatcagcag aaaacccaga aaaatggaaa acgttaaaac
     3721 ataaaaccag ctgaactcgg taaaaatgac ttcttttgcc cggtcgagcc acatctgcac
     3781 ctgatccggc acggttaaaa ccaataccac caaaacacac acaaaaaaca aagtcgtcaa
     3841 aataaccatc ggattaaatg acgttcggcg ttctataaat tcagacaggg acaaaccttc
     3901 tcactccttt gttaaaaaca gacaaacccg gtcatcgggc aaagtagtca aaacctgccg
     3961 gcaaaatacc ggcttccgga tgacgaaatg cacaaaccgg ccgaaa
//
LOCUS       DAXFHF010000101         3757 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXFHF010000101 DAXFHF010000000
VERSION     DAXFHF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.101"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            89..511
                     /locus_tag="ACFIRM_001971"
                     /pseudo
     CDS             89..511
                     /locus_tag="ACFIRM_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(566..1021)
                     /locus_tag="ACFIRM_001972"
     CDS             complement(566..1021)
                     /locus_tag="ACFIRM_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC3029520.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1277..2647)
                     /gene="ffh"
                     /locus_tag="ACFIRM_001973"
     CDS             complement(1277..2647)
                     /gene="ffh"
                     /locus_tag="ACFIRM_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC3029521.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGIK
                     GMFPGL"
     gene            2867..3673
                     /locus_tag="ACFIRM_001974"
     CDS             2867..3673
                     /locus_tag="ACFIRM_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC3029522.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          828 a          930 c         1012 g          987 t
ORIGIN      
        1 aaaggtcagt ttcgatatat ccaatcctac ggcgttacgc atgggattac ccttatatat
       61 tgcgtcccta agaaggggcg caatatataa ggtgtggcaa tttgtcgaag acgcgctgcg
      121 tgccgtcgtg cctgccgaca gttttgaacc gaccgcgcaa aaattgaagc tgtttaaggc
      181 gggcgcggca accattttgt tttatgaaga tcaaaatgtc gtcaaaggtt tgcaggagca
      241 gttccctgct tatgccgcca actttcccgt ttgggcggac caggcgaacg ctatggtaca
      301 gtatgccgtc tggacgacac ttgccgcggt cggtgcaggt gcaaatctgc aacattacaa
      361 ccccttgccc gatgcggcga ttgctaaagc gtggaatatt cccgaaaact ggctgttgcg
      421 cgcgcaaatg gttatcggtg gtattgaagg ggcggcaggt gaaaaggtct ttgaacccgt
      481 tgcggaacgt ttgaaagtgt tcggcgcata atttcgcggt caaaaaaatg ccgtctgaac
      541 cccgttcaga cggcattttt cagtatcagg cggcgagttt ttcgcattct ttgatacagt
      601 cgaggcagga ctcgtaacag gctttgcatt ccgcatggtg ggcggagtgt tctttacagg
      661 ctttggcaca ctgtttgcac gcttcgaggc acacttttgc cgcgtcgcgt gtcagagggg
      721 aattttgtgc ggcaaggtcg tgcaccgcgc cgcataaggc aagcatctgg cgcacggcaa
      781 ccgcacagtc ggacatagac gtgtcgccct gagtgagcag ggacaggcag tgggtcaggc
      841 aaacctgtcc ggcatccaga cagtgtgcgg cagtttggcg gacggcggtg taggcggatg
      901 cggcggcagg ctgcatatcg tgatgatggt ggtagtcggc gtgtccgtgc gcacgcgcga
      961 aagaggcggc ggaagccaaa gagacggcag cggcgctgcc caaaaattga cgacggttca
     1021 tgattttttc ctttatgttt caacatgcgg gtccggcata aggtacacac ttaccatact
     1081 gccctggccg gatcggtacg attttctgaa taattgcttg attttgcagg tgtgaaattg
     1141 tcgcacgagc aggcatgttt tagctattcg gaaattcgag gaagggtgca tcaacctgtt
     1201 ttgaaatgat gcgcagacat ggtaacagac aaaaagccgt ctgaaaatcg ttttagacgg
     1261 cttttgtttt caaacattaa agaccgggaa acattccctt tattcccttc atgcctttgg
     1321 ccaagcgcat cagtttgccc aaaccgttgc cgctgaacat cttcatcatc tgctgcattt
     1381 gttcaaactg tttgagcaat ttgttcactt cctgcacggt cgtgcccgcg cccatcgcaa
     1441 tacggcgttt gcggccggct ttgagcaggg cagggttggc gcgttcttta ggggtcatcg
     1501 agttgatgat ggcttctact ttgcccatcg ctttttcagc cgtaccttcg gggatttgtt
     1561 tcgatatttg acccagttcg cccggcattt tcgacatcag gttttccaaa ccgcccatat
     1621 tgcgcatttg ctggatttgt tctttaaaat cattgaggtc aaagcctttg cctttttgca
     1681 gctttttcgc cattttggcg gcggcttctt catcaatacc tttttgaacg tcttcaatca
     1741 gggtcaatac gtcgcccatg ccgaggatac ggctggcaag acggtcgggg tggaaaggtt
     1801 cgaggccgtt gattttctcg ccgacgccga taaatttaat cggtttgccg gttacatggc
     1861 gtacggacaa tgccgcaccg ccgcgcgagt cgccgtccat cttggtcaat acgacgccgt
     1921 tcagcggcag ggcttcatta aatgcctgag cggtatttac cgcatcctga cccagcatgg
     1981 catcgataac gaacaaagtt tcaaccgggt tgaccaccgc atgaagcgct ttgatttcgt
     2041 tcatcatctc ttcatcgatt gccaaacggc cggcggtatc gaccatcaat acatcgtaaa
     2101 aatgtttttt ggcgtaatca acggcggcag ttgcaatctc aaccggtttt tggttggtat
     2161 cggatgggaa aaaatctacg ccgacctgtt cggccaacag gcgcagctgt tcaatcgcgg
     2221 cagggcggta aacgtcggcg gataccacca aaactttttt cttctgatcg tttttcagca
     2281 ggcgggcgag tttgccgacg gttgttgtct taccggcacc ctgcaaaccg gccatcaaaa
     2341 tgatggcagg cggtgaaacc gataaatcca gtgttttgtt ttccttgccc atcagttcga
     2401 ctaaggcttg gttgaccacg ccgataaagg cttggtcagg cgtcaggctg cccgccactt
     2461 cctgaccgag ggccttttct ttgacgttgt tgacgaactc tttgacgaca ggcagggcga
     2521 catccgcctc aagcagggcg agtcggactt cgcgcaaggc ttctttaata ttgtcttcgg
     2581 tcagtttggc ctgcccccgg atgtttttga agacattgct gaagcggccg gttaaattgt
     2641 ctaacatact ggtccttggt ctgaataaga atagcttgcc ccatcagggg cattctttgt
     2701 taaaataaaa tcaaaataat ttgatgcggc ttgtgtgccg gacagcatat cggcaaatcc
     2761 gtcaaggctt gcccgaaacg gggattttac aattccaacg ttaaaagttc caatatttca
     2821 taagcggccg catacggcgc aacagtatag atagagaaag tccaccatgc cgacagtttt
     2881 catctttttg acggcggttt acgcaggatt gggtgcattt gcatggcact gccaacagca
     2941 ggggcgcggc cgggattacc cgtggaagac ggaattgccg gttttgggcg cggcattgac
     3001 cgtccacggc gcggcactgc tgatgccggt cattcaagac aaaatcatca ttatgggctt
     3061 cgggtattcc ggcagcctga ttgtttggat gatgctgttt atttattttg ccggcagctt
     3121 tttttatccg ctgcgcggag tgcagttgct gctgtatcct tgcgccgcac tgatgctgct
     3181 gtcaggtttg gttttcccgg gaaaattctc gggatatgaa attaccgact ttccctttat
     3241 gctgcatatc ggcacatcac tgcttgcata cgggctgttc ggcatcgcaa cattattgtc
     3301 cgttttgagc ttgctgctgc accggagcct gcaccgcaga aacttctcca agctcgcagg
     3361 attcctgccg tcgctgctca gtttggaaaa actcatgttc caggccatgt gggcaggttt
     3421 catcctgctg acctattccg tcgtcagcgg cacattcttt gccgaagccg tattcggcaa
     3481 acccatgaca tttacccata aaaccgtatt cggcatattg tcatggctga tttacggagg
     3541 actgctgctc aaacacagca tgaccgcatg gcgcggcaaa aaagccgccg tgtggaccat
     3601 catcggattt gtcagcctta tgattgccta tatgggcagc aagttcgtat tggaaatcat
     3661 tctgaaaaga taagaagagc caacagatgc cgtctgaagc cccgagtttc aggcggcata
     3721 ttcacaaagg cgcatcagcc ggaggagaag agggggt
//
LOCUS       DAXFHF010000102         3714 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXFHF010000102 DAXFHF010000000
VERSION     DAXFHF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.102"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(45..227)
                     /locus_tag="ACFIRM_001975"
                     /pseudo
     CDS             complement(45..227)
                     /locus_tag="ACFIRM_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(395..988)
                     /gene="ribA"
                     /locus_tag="ACFIRM_001976"
     CDS             complement(395..988)
                     /gene="ribA"
                     /locus_tag="ACFIRM_001976"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC3029523.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            complement(981..1655)
                     /locus_tag="ACFIRM_001977"
     CDS             complement(981..1655)
                     /locus_tag="ACFIRM_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029524.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWRNDEGWKLADYLPCDDVRENALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1806..1890
                     /locus_tag="ACFIRM_001978"
     tRNA            1806..1890
                     /locus_tag="ACFIRM_001978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Leu,seq:tag)
     gene            2409..3443
                     /gene="purM"
                     /locus_tag="ACFIRM_001979"
     CDS             2409..3443
                     /gene="purM"
                     /locus_tag="ACFIRM_001979"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC3029525.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT          960 a          971 c          885 g          898 t
ORIGIN      
        1 aaatgtcagc ttggatatgt ctaatcctac ggcgttacgc atgggattac ccttatatgc
       61 ggatatttgc ctgcatattc gtcgcaaatc ttcccgcagc cgtccggcga accgtcatcg
      121 accaaaatca tttcataatc ggcaaaattt tcggcaagca cggaatccac gcagcagcga
      181 aggtattttt ccacattgta aatagggacg atgatagaga aaatcataaa tatcaatacg
      241 ttgtattaag atgtttgcgc gtatgcctca aacccgcgct cgcaatgcgt ttgcatccgc
      301 accctgcaac ttgataaaac ttattgcttt atcaagctat ggaaacctgt ttcccgaaag
      361 acggcgcagg atgcccgttc cctgcaactt tgccttattc cgacatcaaa tgccccagct
      421 tgtctgcttt ggtttggaga taacgctcgt tttccagatt ttccccgacg tgcaggggaa
      481 tgcgttcgac cacgttaatc cccgcatctt tcagggtttg gattttttcg gggttgttgg
      541 tcaacagttt gaccgagcgg atgcccagat attcgtagat agattgcgcc aaacggaaat
      601 cgcgggcatc gacgggcagc ccgagtgcca aattggcttc aacggtatcc ataccttgtt
      661 cttgcagatg ataggcgcgg attttgttaa tcagcccgat gccgcgtcct tcctgacgca
      721 gatagacgat gatgccgcgc ccctctgcct gtaccgccct cattgccgct tcaagttgcg
      781 gtccgcagtc gcattttctc gagaacagcg cgtcgcccgt cagacattcg gagtggatgc
      841 gcgtcaacac cggattgccg tctgaacaat tgccgacggt cagcgcgacg tgttcctgcc
      901 cgtttgcctc ttcaaaaccg tgcatcgtaa atacgcccca ttcggtcggc agacggcagg
      961 aagcgacatg gtctaataat tcagacattt tcatctcctt ctgcggcagt gatgcccaaa
     1021 agttgcttca aaggttcgga caaagccaag gcaagagcca cccattccgc ctgcgccgcc
     1081 gtatccgcac actctgcgca ttcaaattcc acatgaacca cgcccaatac accgccgctc
     1141 tccgtgcaaa cgggaatgga aatttgcgcc gccgaagcat gattgcgttc tcccgaaagc
     1201 tcacccaaat tcaaccaacg gcgtacatcc gaggcaacat tcatccaacc gctttgcgcc
     1261 gaacggcagg caagcgaaac atcggtaata tttccatcct gctcccatat gttttccaaa
     1321 ccttcgccct gttgggacag gcacaccagc cggagggcgg cgttttcaga cggcatcaag
     1381 gcataaaccg ccgcgctccg cacacctgtc gagcgtgaaa acaccgaatc caaagccata
     1441 aaaagccgtt tcagggcatt ttcgcggaca tcatcgcacg gcaggtaatc ggcaagtttc
     1501 caaccctcat cattgcgcca caaaacagaa cggtcaatcg aagccatccc catatccatt
     1561 accgtctgcg ccgtcagata cgccgcccga acctcgtcaa gcggcagctt caaaccctga
     1621 gtcagcagaa aatccttaat caaagcagca ggcataccca aattccgtca ataaaaacaa
     1681 aaaaccgccc gattccgatg ccaggcggac gaatagcgga ttttaccgct gcaaagcata
     1741 agcctcaact attacgtcca aagccttgcg caatcggaca aaatctgtat aatctccacc
     1801 cttatgcgga cgtggcgaaa ttggtagacg caccagattt aggttctggc gccgagaggt
     1861 gtgagagttc gagtctctcc gtccgcacca ataaaaatat ataaatcata ttgttaatga
     1921 ttattgggac aattcggtgg cacaaaatat aagctgtctg cttttcagcg ggcggctttt
     1981 tatctgccta tgtgtttcaa cacacaaaac ggcacataaa gcaccgccat ttgttccgca
     2041 ccgaatcgga agcgggtgct tccgcccgca atcggattcg ccgcccctaa tgtcggggtt
     2101 ttaaccggaa agaagaaaat attgattttg atgatggcat caaaaaaacc cttccgcccg
     2161 tctgccaaaa ggaatggtta agaaaagcaa tgcgtctttt gatggaacgc gatatataag
     2221 aaattgaggg gaagggcagg cgggtttctg cccgttaatc ctgcggacgg ccccgccaaa
     2281 cccgacacgc gcgggcatat ggcggattgt ttacaatatt gtctaaaaat ctgaacctgc
     2341 aaacgcgaaa taaggtagaa tacgccccgt tattttaccg tccaatcccc atttaccaag
     2401 gaaaaacgat gagtacttca ttgagttacc gcgatgcggg cgtcggtatc gacgcaggcg
     2461 accaactggt cgaaaaaatc aaaccgtttg ccaaacgcac catgcgtccg gaagtattgg
     2521 gggatttggg cggttttggc gcattggtcg aaatcggcaa aaaatatcaa aaccccgtat
     2581 tggtcagcgg tacggacggc gtgggtacca agctcaaact tgccttcgat tgggacaaac
     2641 acgatacggt gggcatcgac cttgttgcaa tgagtgtcaa cgatattttg gttcaaggag
     2701 ctgaaccatt gtttttcttg gattattttg cctgcggtaa attggatgtt ccgcgtgcga
     2761 ccgatgtcat caaaggcatt gcccaaggtt gcgaagaatc cggctgcgcc ctgattggcg
     2821 gggaaactgc cgaaatgccg ggtatgtatc ccgttggtga atacgatttg gcgggttttg
     2881 ccgtcggcgt ggtggaaaaa gagaatgtca ttaccggccg cagcatcggc gcgggcgatg
     2941 tggtattggg tttggcttcc aacggcgcac attcaaacgg ctattccctt atccgtaaaa
     3001 tcatcgaacg cgacaatccc gatctggatg ccgagtttga taatggcaaa actttacgtg
     3061 aagccgttat tgcgccgaca cgtctgtatg tgaaaccgat tcttgccgct ttggaaaaat
     3121 ttaccattaa aggtatggcg cacattaccg gcggcggcat taccgaaaac gtgccgcgcg
     3181 tgttgcctaa aaacacggtt gcccaaatcg atgccgaatc gtgggaattg cccaagctct
     3241 tccaatggct tcaaaaggcg ggcaatgtgg aaacccaaga aatgtaccgg acctttaact
     3301 gcggcatcgg tatggtcgtc atcgttgctg ccgaagatgc cgatgcggtt cggtcattcc
     3361 tgagcgggca gggcgagaca gtgtaccgtt tgggttgcat ccgagagcgt caagggaacg
     3421 agcatcaaac ccaagttgcc tgacttccaa gcatcaaatg ccgtctgaat ttgtttcaga
     3481 cggcatttcc gcatcttcca aacgcgtatc cgcaacacag ttctgcaaac tgatattttg
     3541 cccggtatgg cggcttaaac ttcttcatca tatttcgata atgctataaa atgatataat
     3601 cataaaatat tgtttattcc tacatcgtat tcatcctatt ttccgaaatg tgagcgtttt
     3661 atgaagcaat ccgtccataa taaaacaatt ttaagtgttt tgatttcatc tatg
//
LOCUS       DAXFHF010000103         3655 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXFHF010000103 DAXFHF010000000
VERSION     DAXFHF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.103"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..344)
                     /locus_tag="ACFIRM_001980"
     CDS             complement(<1..344)
                     /locus_tag="ACFIRM_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3029526.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALALVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQHLTVRHVSTGASSPGKVSAVVSSSVS
                     RAGVLSGVGKLVR"
     gene            complement(691..993)
                     /locus_tag="ACFIRM_001981"
     CDS             complement(691..993)
                     /locus_tag="ACFIRM_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029527.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLMINFLKDIGKVGTD"
     gene            complement(1021..1251)
                     /locus_tag="ACFIRM_001982"
     CDS             complement(1021..1251)
                     /locus_tag="ACFIRM_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029528.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDAAEIGKQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1478..1678)
                     /locus_tag="ACFIRM_001983"
     CDS             complement(1478..1678)
                     /locus_tag="ACFIRM_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029529.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            complement(1682..1993)
                     /locus_tag="ACFIRM_001984"
     CDS             complement(1682..1993)
                     /locus_tag="ACFIRM_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029530.1"
                     /translation="MFNQNQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSRNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(2005..3219)
                     /locus_tag="ACFIRM_001985"
     CDS             complement(2005..3219)
                     /locus_tag="ACFIRM_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC3029531.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFIDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSGKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFIDDSIRPRITRIDLALDFFNGEYTP
                     DQALLDHDNGFFDNSNQRPKSEMIGTAWRNEDGTGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPFPERFNQRNKT
                     LNLTFEHKLRYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDAEKERKYQDYLD
                     EVYHQNVNYDYF"
BASE COUNT         1151 a          837 c          562 g         1105 t
ORIGIN      
        1 cggacaagtt tgccgacccc cgacaatacg ccggcgcggg atactgatga agatacaacc
       61 gcactaactt taccaggact tgatgcccca gtagatacat gcctaactgt taaatgttga
      121 gcttctgaag ttaaatatct tcccgattct ctatcaaata cacctttcca aattcttctt
      181 cttttgtcat ctgaacctcg aatacctaaa tatctgccat catcataaac ttcaacccta
      241 tcaaatttac ccggaacatt tactaatgcc aatgcaaatt taggtacaaa aaaactaaag
      301 cataggataa tctttaaaaa atttcgccct aggataagaa acataacatt ttgcttcatc
      361 taatttaatt aataatgtag aagaatcagg gaattgaatt tctataaaat ctctatcata
      421 acgatgaaag aaattcctaa aaatatcaaa tgaaaacgaa gatttaaaaa ccccttttct
      481 aatcaattcc ctataataaa ttaacgcttg tggtaaatct ctataaatat gtgaatcagt
      541 ttgaaattta actaaatgat attcagaaat aaacataatt cggacctttt aaaacactct
      601 aaaaatccga ttctaccaac cgttaccaaa acagccaagc aacagacaga gccaagaaga
      661 aaccgagaag aaaccagaaa tcaataacca tcaatcagtc ccaaccttgc ctatgtcttt
      721 taaaaaatta atcatcagcc taaagccgta aatgactacg aacagaatta gaaccgtcga
      781 accgacataa gaaccttgct ttatctgctc aaaattggaa cattccggat aagacaacgt
      841 aaccggcttt ccgttcaaaa tccatttatc gcccaccctt tccggcctga tgatttttcc
      901 gtcctgggta acagtaggag gaagggacga caataaatag tcgtctgcct gcaatcttgt
      961 atcaaaacaa tttatgccga cacgatagcc cattcatgcg cccctttttc ttcactctgt
     1021 ttatttgaca gatttaatca tgctccaagc cattttgaag ccttggattg caagaatcac
     1081 ggtaatggcc gccataccca cggcggaaac cattgacacg aaacccatga ttacattcgc
     1141 tacttgctta ccaatttcgg ctgcatcaaa ggtatctgcc ataacaatgg ccggtgtgaa
     1201 gataccggct gccaaggctg cttttacagc gtatttttta acgatgttca tcgttttttt
     1261 ccttttttga tatttaaagt aagacggctt cttaggttta aatccgggcg aagcctgctc
     1321 ccgaattttg tttttaattt atgaaataga gaattgagaa aatgaaaaga atgaggcaaa
     1381 caaaaaatcc gataattaaa gttgctttat tcatttttaa tcctttttgc gggctttgtg
     1441 aaaggttgac agaccgcccg ccgagcctgt tttactttta ttccgatttc acgaagaact
     1501 gaaatatttg gaatcctccg cctatttcat ttatgcctga gttcaatgca tcttcgtaac
     1561 tttcaaattg acctgctgat ttaatatttt gagtaaaccc cacgtcaccg aacggatcgg
     1621 gataaataaa gtcatgcgtt tctaaatctt gaactatgaa acgttcttca aatttcataa
     1681 atcaaccttt cggtttttcc gccacctgaa aatcaattaa tgaaggaacc atgcctttac
     1741 ctgtcgaagt catttcaacc gttaccataa cttcgcacgg gtatttgaga ttctctaatt
     1801 ttgagaaatt cctactgtcc ccgaatttca tttgtgccgc cgtgaatccg acagcattcc
     1861 ccgactgtgc cggtaaaggt gttgcaacca acacagaaca agtatctata ttagagccgt
     1921 caatttcgcc tttgaatttt ttagctccca aaaaagtcgc aggataagtt acagtttggt
     1981 tttgattaaa cattttgatt tcctttaaaa ataatcataa tttacatttt gatgataaac
     2041 ttcatctaga taatcctgat atttcctttc tttctcggca tcataatcag gactaaaaag
     2101 ccttttttcc ctatcgaatt tgtcagaatt tttaaaagca ataaccccca attcatcaag
     2161 ttcaatttcc aaatcaatat ccggctgttc atgaataaaa ccgtgtttta acccgtccct
     2221 taacatttcc agagcatatt tttcaggttc taacccttta ggaaatcccg aatctgcctt
     2281 taaagattcc acaatttcac tattatcaaa acccatttca atcatgaaat tgaccagttt
     2341 tccaaccgcg ttttttgcat aacgcaattt atgctcaaaa gttagattaa gcgttttatt
     2401 tctctgatta aacctttcgg gaaacggcat atttttaaat tttctacaaa ttggaaaagc
     2461 tccacagaaa tacgaaccct gatttattaa aatatccaag ggtatttcaa tatctccata
     2521 attaaactgg atttcgaacc ttacccactt gctttcttta tcgccaagct gcctgccttt
     2581 ctcataaaca cgaacaaaac gagaattttt cttgcgacct acataaaatg tctttccggt
     2641 cccgtcctca ttccgccaag ccgtcccgat catttcagat ttcggccttt ggttactgtt
     2701 gtcgaaaaac ccattatcgt gatctaacaa tgcctgatcc ggcgtgtact ctccgttaaa
     2761 aaaatcaagt gctaggtcaa ttcgcgttat tcttggcctt atcgaatcat caataaactg
     2821 ctttaacctc aactcccaac ccggacttgc aacgctgcaa ccagtacctt tcaactcaac
     2881 tagaacagta ttccgctgtc ctccgaaatg cacctctcca taatcaacat catccgaacc
     2941 tagcctatac atggattcat agaatttgtt gccccttgat ttgcatttgc gcgttatgcc
     3001 aaaacctaga atttcttcca gctttccgct taatacatac atgtattcag catcagaaaa
     3061 taaaggacac ccggaaactt tcagtaaagt atcttcatgg aatgtgaatg aaatccaatc
     3121 aataaaaaca ccgtcttgct tacctcttcg ctgcggaatt tctaaaagct ttccctttcc
     3181 gtccgatata aagtgtgaga aatattcaac ttcactcatt cgttcactac tcttggaatt
     3241 tggtttttaa tttcgctccc cccctgttag tcaagggggg ggctttcagc cgtttcccgt
     3301 cttccgcgct aaagcgcgta cgacggtcaa cggctgaaag ccaaatcctg acaaactgtt
     3361 aaagatcaac aaaaaagacc acaaccatcc gttgtgataa tcactggaaa attcgaacca
     3421 accgaatcta tataatcgaa cgcctgataa agctttgaaa aattctcttg attaatgagt
     3481 ttatgcggtt cgccatgtct gaactgataa aaacacaaaa cacaagaatc tgatttttta
     3541 aatattctcc agtaagaaca ggagaatatt acatttgctg ctgacataaa aaagcccctt
     3601 tcacttggct gtcaaagggg aatgttaaga aaagtaatgc gcccctttga tagag
//
LOCUS       DAXFHF010000104         3581 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXFHF010000104 DAXFHF010000000
VERSION     DAXFHF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.104"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            129..3328
                     /gene="nalP"
                     /locus_tag="ACFIRM_001986"
                     /pseudo
     CDS             129..3328
                     /gene="nalP"
                     /locus_tag="ACFIRM_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
     gene            3380..>3581
                     /locus_tag="ACFIRM_001987"
     CDS             3380..>3581
                     /locus_tag="ACFIRM_001987"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029532.1"
                     /translation="MKRKPYSNCLLLLFQDFFQRFGINQTYSGLTKIRTRRRAADGTN
                     GTEPIRPVLQHLREPFPLSRGGA"
BASE COUNT          963 a          977 c          973 g          668 t
ORIGIN      
        1 aaaatcgagt ttatcctagt tgtccaggac agatataatt caaaatataa aaatgggtta
       61 catctggaca ttacgaaatt tttattcctt cgcctgaatt ctattgtcag attcaaagag
      121 acctcatcat gcgaaccacc tcaaccttcc ctacaaaaac tttcaaaccg gctgccatgg
      181 cgttagctgt tgcaacaaca ctttctgcct gcttaggcgg cggcggaggc ggcacttctg
      241 ctcccgactt caatgcaggc ggcaccggta tcggcagcaa cagcagggca acgatagcgg
      301 aatcagcagc agtatcttac gccggtataa aaaacgaaat gtgcaaagac agaagcatgc
      361 tctgtgccgg tcgggatgac gttgcggtta cagacaggga tgccaaaatc aaagcccccg
      421 aatctgcata ccggagactt ttcaaaccca aatgaccaat attaagaata tgatcaacct
      481 caaacctgca attgaagcag gctatacagg acgcggggta gaggtaggta tcgtcgatac
      541 aggcgaatcc gtcggcagca tatcctttcc cgaactgtat ggcagaaaag aacacggcta
      601 taacgaaaat tacaaaaaca aattacaaaa actatacggc gtatatgcgg aaggaagcgc
      661 ctgaagacag aggcggtaaa gacattgaag cttctttgga caatgaggcc gttatagaga
      721 ctgaagcaaa gccgacggat atccgccatg taaaagaaat cggacacatc gatgtgatct
      781 cccatattat tggcgggcgt tccgtggacg gcagacctgc aggcggtatt gcgcccgatg
      841 cgacgctaca cataatgaat acgcatgatg gaaccaagaa cgagaaataa tgtctgcagc
      901 catccgcaat gcatgggtca agctgggcga acgtggcgtg cgcatcgtca ataacagttt
      961 tggaacaaca tcgagggcag gcactgccga ccatttccaa atagccaatt cggaggagca
     1021 gtaccgccaa acgttgctcg actattccgg cggtgataaa acagatgagg gtatccgcct
     1081 gatgcaacag agcgattagg gcaacctgtc ctaccacatc cgtaataaaa acatcctttt
     1141 catcttttcg acaggcaatg acacacaagc tcagcccaac acactgaccc tattgccatt
     1201 ttatgaaaag acgctcaaaa aggcattatc acagtcgcag gcgtagaccg cagtggagaa
     1261 aagttcaatg gctccaacca ttgcggaatt actgccatgt ggtgcctgtc ggcatcctat
     1321 gaagcaaccg tccatttcac ccgtacaaga ccgattcaag ttgccggaac atccttttcc
     1381 gcacccatcg taaccggcac ggcggctctg ctgcagagat acccgtggat gagcaacgac
     1441 aacctgcgta ccacgctgct gacgacggct caggacatcg gtgcagtcgg agtggacagc
     1501 aagttcggct gggggctgct ggatgcgggt aaggccatga acggacccgc gtcctttccg
     1561 ttcggcgact ttaccgccga tacgaaaggt acatccgata ttgcctactc cttccgtaac
     1621 gacatttcag gcacgggcgg tctgatcaaa aaaggcggca gccaactgca actgcacggc
     1681 aacaacacct atacgggcaa aaccgttatc gaaggcggtt cgctggtgtt atcggatatg
     1741 cacgtcaaaa ccaaaggtgc actgatttat aacggggcgg catccggcgg cagcctgaac
     1801 agccacggca ttgtctatct ggcagatacc gaccaatccg gcgcaaacga aaccgtacac
     1861 atcaaaaggc ggtctgcagc tgggcgacaa aggtacgctg tacacacgtt tgggcaaact
     1921 gctgaaagtg gacggtacgg cgattaccga cggcaagctg tacatgtcgg cacgcggcaa
     1981 gggggcaggc tatctcaaca gtaccggacg acatgttccc ttcctgagtg ccgccaaaat
     2041 cgggcaggat tattctttct tcaaaaatat cgaaaccgac ggcggtctgc tggcttccct
     2101 cgacagcgtc gaaaaaacag cgggcagtga aggcgacacg ccgtcctatt atgtccgtcg
     2161 cggcaatgcg gcacggactg cttcggcagc ggcacattcc gcgcccgccg gtctgaaaca
     2221 cgccgtagaa cagggcggca gcaatctgga aaacctgatg gtcgagctgg atgcctccga
     2281 atcatccgca acacccgaga cggttgaaac tgcggtcgcc gaccgcacag atatgccggg
     2341 catccgccta cggcgcacaa ctttccgcac agcggcagcc gtacagcatg cgaataccgc
     2401 cgacggcgta cgcatcttca acagtctcgc cgctaccgtc tatgccgaca gtgccgccgc
     2461 ccatgccgat atgcagggac gccgcctgaa agccgtatcg gacgggttgg accacaacgg
     2521 tacgggtctg cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg aacagggcgg
     2581 tgtcgaaggc aaaatgcgcg gcagtaccca aactatcggc attgccgcga aaaccggcga
     2641 aaatacgaca gcagccgcca cactgggcat aggacgcagc acatggagcg aaaacagtgc
     2701 aaatgcaaaa accgacagca ttagtctgtt tgcaggcata cggcacgatg tgggcgatat
     2761 cggctatctc aaaggcctgt tctcctacgg acgctacaaa aacagcatca gccgcagcac
     2821 cggtgcggat gaatatgcgg aaggcagcgt caacggcacg ctgatgcagc tgggcgcact
     2881 gggtggtgtc aacgttccgt ttgccgcaac gggagatttg acggttgaag gcggtctgcg
     2941 ccacgacctg ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg gctggagcgg
     3001 caacagcctc actgaaggca cactggtcgg actcgcgggt ctgaagctgt cgcaaccctt
     3061 gagcgataaa gccgtcctgt ctgcgacggc gggcgtggaa cgcgacctga acggacgcga
     3121 ctacgcggta acgggcggct ttaccggcgc ggctgcagca accggcaaga cgggtgcacg
     3181 caatatgccg cacacccgcc gggttgccgg tctgggggtg gatgtcgaat tcggcaacgg
     3241 ctggaacggc ttggcacgtt acagctacac cggttccaaa cagtacggca accacagcgg
     3301 acaaatcggc gtaggctacc ggttctgacg gacagaaaac agacagccgc aaagatcacg
     3361 gtctttgcgg ctgtttctta tgaaaagaaa accctattcc aattgcctgc ttctattgtt
     3421 tcaagacttc ttccaaagat tcggcatcaa tcagacgtat agcggattaa caaaaatcag
     3481 gacaaggcgg cgggccgcag acggtacaaa tggtacggaa ccgatccgcc cggtgcttca
     3541 gcaccttagg gaaccgttcc ctttgagccg gggcggggca a
//
LOCUS       DAXFHF010000105         3357 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXFHF010000105 DAXFHF010000000
VERSION     DAXFHF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.105"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            375..450
                     /locus_tag="ACFIRM_001988"
     tRNA            375..450
                     /locus_tag="ACFIRM_001988"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:409..411,aa:Glu,seq:ttc)
     gene            complement(666..818)
                     /locus_tag="ACFIRM_001989"
     CDS             complement(666..818)
                     /locus_tag="ACFIRM_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029533.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            878..1684
                     /gene="thiD"
                     /locus_tag="ACFIRM_001990"
     CDS             878..1684
                     /gene="thiD"
                     /locus_tag="ACFIRM_001990"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC3029534.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(1742..2611)
                     /gene="tehB"
                     /locus_tag="ACFIRM_001991"
     CDS             complement(1742..2611)
                     /gene="tehB"
                     /locus_tag="ACFIRM_001991"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC3029535.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            2724..3161
                     /gene="rnhA"
                     /locus_tag="ACFIRM_001992"
     CDS             2724..3161
                     /gene="rnhA"
                     /locus_tag="ACFIRM_001992"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC3029536.1"
                     /translation="MDTTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT          798 a          970 c          824 g          765 t
ORIGIN      
        1 acttcggttc cgccattaag ggtatagttg ttctcttgcc cttgttttgc gaggacgagg
       61 gtgtatttgt ctgtcttctc tatcgttccg ctgtttgcag cggccgcccc ttgcgcgtgc
      121 atagaagcgg ccgcgaagcc aagtaagatg cactgaactg tcttcttttg tttcatcggt
      181 ttttccattt tgaaggtatg tgatttttat cattatatgc ttaaatcatt gacacgcccc
      241 caaaaaaaaa atgaattgct tgatattcca atgtttttta tcttgtgata cggtcagaca
      301 aacgccccct gacattcgtt tagacggcat cgtattgcta aatttctata agtatgtata
      361 atgtccgttt ccacgcgccc atcgtctaga ggcctaggac actgcccttt cacggcggca
      421 accggggttc gaatccccgt gggcgtgcca attcaaaaac ctgcttgttt caagcaggtt
      481 ttttattatg aatcgttatt cccgcaattt ttcgtcattg ccgtgaaaac gggaatctag
      541 aacctcaaac tttcagataa tctttgaata ttgctgttgt tctaaggtcc ggattcccgc
      601 ctgcgcggga atgacggaat tttaggtttc tgtttttgcg ggaatgacga atgaagcgtg
      661 ccggtttata ctcgccgcaa cacgcggttc agacggcatc gccctctttt ttcattatca
      721 gttgctacga tgcaccccgc tgccttgccc tgtgccttgt cctgcaatac gacataataa
      781 tgcaccacaa accccgcgct gcggttttca gacggcattg cctgcttttt tacaggcagt
      841 agcccttttt atcggacgca atattaagga ggaacaaatg aaaggctctt ttgtgcagac
      901 gcttgccatc gccggttcgg attcgggcgg cggtgcgggc attcaggcgg atttgaaaac
      961 gtttcagatg cgcggcgtgt tcggaacgtg tgtcatcacc gccgttaccg cgcaaaatac
     1021 cttgggcgtg tcggcggttc atctcgtccc gaccgaaacc atcaccgcac aaatccaagc
     1081 aatcagggaa gactttgaca tccgcgccta caaaatcggt atgctcggca cggcggaaat
     1141 catcgaatgc gttgccgaca agctgaaaca ctgcagcttt ggcaggcgcg tactcgaccc
     1201 tgtgatgatt gccaaaggcg gtgcgccgct gttgcaggat tccgccgttg cggcactgac
     1261 gcgcctgctg ctgccggaca cggacatcct gacccccaac ctgcccgagg cggaagcctt
     1321 gacgggcgtg catatcgaaa accgtaaaga tgcggaacgt gcggcaaaaa tcctgcttga
     1381 ttacggtgtc aaaaatgtca ttatcaaagg cggacatttg aacggcagca caagcggacg
     1441 ctgcacggat tggctgttta cgcaaaacga aacgctggaa ttggacagcc cgcgctttcc
     1501 gaccgcccac acgcacggca cgggctgcac gttttccgcc tgcattaccg ccgagttggc
     1561 aaaaggctta gacgtttgca aagccgtaca gactgccaag gcctacatca cggcggcaat
     1621 ctcaaaccct ttggaaatcg gcgcgggaca cgggccggtc aatcattggg cgtatcggga
     1681 ctagacgtaa aaatgccgtc tgaacgcctt ttcgggttca gacggcatcg tccggcgcgg
     1741 cttatcctgg ttttttcgcc agcatagtta caaatttaaa ccgtatcggg ttgccgtttt
     1801 cgtctttggc gtgcatcgcg cccaattctt ctttatattc gaccagctcc caatcccgat
     1861 aataatcctt cagctcgccc tctttaaatt tgaaggggaa cggcatcggg caggggaaat
     1921 ccgccgtatc cattgccgat acgattaagt tgtacccgcc cgccgccgta tgcgcctgca
     1981 tatcggcaat cacgtcgggc acgcgctgcg gcatcaggaa catcagcacc accgttgcca
     2041 caatataatc aaactcgccc tgaagggcgg cggcgttcaa atcatattcc agcgtgcgga
     2101 cgttcaaacc ctccgcctct gccagttccg ccacgtttgc caacgcggcg ggattgcaat
     2161 cggcagcggt tacatcaaac cccttcaaac ccaggaacag cgcgttgcgc ccctgtccgc
     2221 agcccatatc caacgccctg cccgccggta cggtatcctg tgccgccgcg accgcagaat
     2281 gcgtggcact catcccgtat tttttgtgaa aatagtctgc cgccgcgcaa tacagcgaca
     2341 aacggatttc ggcatcgtcc gttttcggtt tgaccgaaaa cacctgctgc ggcgcaaaca
     2401 cgcaatcgcc gctttccgcc gaccaaacct ctgccgcccc gtccggtgca cggacttcga
     2461 catcgccctg caacacattc aggcagaccc attccccttc ctcggacgaa taacccgaca
     2521 acaaaacttc cggcaggttt tccactttcc atacaggcat ttgtccgaaa caaaacaact
     2581 cgccactttg acccactatc cgctccttca tattcaaaaa taaagttgca cattatatgc
     2641 ctatttcaat ccgccgcaat ctttcagacg gcacgacgca caaaccgctt ataatcacgc
     2701 cggacaccac acaaaggcac gatatggaca caaccgttta cctctacacg gacggcgcgt
     2761 gcaaaggcaa tcccggcgcg ggcggctggg gcgtattaat gcgctacggc agccgcgaaa
     2821 aagaactttt cggcggcgaa gcgcaaacca ccaacaaccg catggagctg accgccgtta
     2881 tcgaagggct gaaatcgctc aaacgccgct gcaccgtcat catctgcacc gactcgcaat
     2941 acgtcaaaaa cggcatggaa aactggatac acggttggaa gcgcaacggc tggaaaaccg
     3001 ccgccaaaca gcccgtcaaa aacgacgact tgtggcaaga actcgacgct ctggtcgggc
     3061 agcatcaagt cagctggact tgggttaaag gacacgcggg acacgcggaa aacgaacgcg
     3121 ccgacgattt ggcaaaccgt ggcgcggcgc agttttcttg attgccgcac cgacaaaaat
     3181 gccgtctgaa accgctaatg ggcttcagac ggcatcgtcc tccaccgtca ttcccgcgca
     3241 ggcgggaatc cagaacgtcg ggcaacggca atattcaaaa gccgtctgaa aatttaaaag
     3301 ttctagattc ccgttttcac gggaatgacg aaaagttgcg gacagcagag gaatgac
//
LOCUS       DAXFHF010000106         3210 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXFHF010000106 DAXFHF010000000
VERSION     DAXFHF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.106"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            43..2301
                     /locus_tag="ACFIRM_001993"
                     /pseudo
     CDS             43..2301
                     /locus_tag="ACFIRM_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            2314..2956
                     /gene="pgmB"
                     /locus_tag="ACFIRM_001994"
                     /pseudo
     CDS             2314..2956
                     /gene="pgmB"
                     /locus_tag="ACFIRM_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            3096..>3210
                     /locus_tag="ACFIRM_001995"
     CDS             3096..>3210
                     /locus_tag="ACFIRM_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC3029537.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYL"
BASE COUNT          819 a          969 c          811 g          611 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttta cgatgtacac aagaattatg
       61 gaaatcagcc cttggacgct gcgttcggca aaactggaaa aagaacacaa acggctgcaa
      121 gagagcctga ccagcttggg caacggctat atgggtatgc gcggcagctt tgaggaaacc
      181 tactccgccg acagccactt gggcacctac atcgccggcg tgtggtttcc cgacaaaacc
      241 cgcgtcggct ggtggaaaaa cggctatccc aaatatttcg gcaaagccat caacgcgctc
      301 aatttcagca aagtcaaaat ctttgtcgac gggcaagagg tggacttggc gaaaaacgac
      361 gttgccggct tctccgtcga actggatatg caacacggcg tgttgcgccg ctcgttcacc
      421 gtattcggtg tgcgtttcga tgtgtgcaaa ttcctgtcgg tcgcgcaaaa agagctggct
      481 ctcacccgct gggaagccgt gtccgttgac ggcaaaaccc accaagtccg catcgattcc
      541 atcatcgatg ccgacgtgaa aaacgaagac tccaactacg aagaaaaatt ctggcaggta
      601 ttggacaaag gcgtttcaga cgaccgctcc tacattgccg cccaaaccgt cgccaaccct
      661 ttcggtgtgg aacaatttat cgtcaacgcc gggcaaacct tcgccggcag cttcaaagcc
      721 ttcggcggca gccaaaccga ttggcaggtc tctaattctt ttgaagccga agtcggcggc
      781 acgcccgaaa cctttgaaaa acgcgtgatt gttaccacca gccgcgatta tcagggcttg
      841 gaagcagtga aagccgccgg ccgcgcctta tctgaaaaaa tcgcaggtgt tgcgtttgaa
      901 accttgctgg atgcgcacaa agcaggctgg cagcatcgtt gggaaatcgc cgacgtggtc
      961 atcgaaggca gcgacgaagc acagcagggc atccgcttca acctgttcca actgttctcc
     1021 acctactacg gcgaagacgc gcgtctgaac atcggcccca aaggctttac cggcgaaaaa
     1081 tacggcggcg cgacctattg ggacaccgaa gcctacgccg tgccgctcta ccttgcactg
     1141 gcggaacccg aagttacccg caacctgctg caataccgcc gcaaccaaat tgccgcaggc
     1201 gcagcataac gcacgcgaac agggcttggc gggcgcactc tatccgatgg taacgtttac
     1261 gggcatcgag tgccacaacg aatgggaaat caccttcgag gaaatccacc gtaacggcgc
     1321 gattccttac gccatctaca actacaccaa ctacaccggt gacgagtgct atcttgccaa
     1381 agaaggcttg gaagtcttgg tcgaagtgtc ccgcttccgg gcggaccgcg tccacttctc
     1441 caaacgcaac ggcaaataca tgattcaagg cgtaaccggt ccgaacgaat acgaaaacaa
     1501 catcaacaac aactggtaaa ccaacaccct cgccgcatgg gtattgggct acacccgcga
     1561 agccttggcg aaatacccgc gtccgggttc gaacgtgagc gccgccgagt tggaaaaatg
     1621 ggcggacatc agcgcgaata tgtaccgtcc gcatgacgaa gaactcggcg tattcgtgca
     1681 gcacgacggc ttccttgaca aagacatccg ccccgtatcc gcgctttcgc ccgacgattt
     1741 gccgctcaac cagaaatggt cgtgggacaa aatcctgcgt tcgcccttca tcaaacaggc
     1801 ggacgtattg caaggcatct acttcttcgg cgaccgtttc gacatggacg aaaaacgccg
     1861 caacttcgac ttctacgaac cgatgaccgt gcatgaaagc tcgttgtctc catgtatcca
     1921 ctccatcctc gctgccgaac tgggcaaaga agaaaaagcc gtggaaatgt accggcgcac
     1981 cgcccgcctg gacttggacg actacaacaa cgacaccgaa gacggcctgc atattacttc
     2041 tatgaccggc tcgtggctcg ccatcgtcca aggtttcgcc caaatgaaaa cttggggcgg
     2101 caaactcagc ttcgcaccgt tcctgccgag tgcgtggaca ggctatgcct tccacatcaa
     2161 ctaccgcggc cgtctgatta aagtcgccgt cggcaaaaaa acgtcgtctt caccctgctc
     2221 aaaggcgatc cgctcgaatt gcaggtgtac ggcaaagata tcacgctcaa cggcagccac
     2281 accgttgcat tggaaaaata aggagggcgc aaaatgactt ttaccgcagt gctcttcgat
     2341 ctcgacggcg tgattaccga taccgccgaa taccactacc tcgcatggaa aaagcttgcc
     2401 gaagaattgg gcatcggcat cgaccgcaag tttaacgagc agctcaaagg cgtgtcgcgc
     2461 gacgattcgc tcaaacgcat cctcgcgcac ggcggcaaaa ccgtcggcga agccgagttc
     2521 gccgaactga cctgccgcaa aaacgacaac tacttggaga tgattcaggc agtcaaaccc
     2581 gaagacgtgt accccggcat tttgccgctg ctggaagcat tgagggcaaa cggcaaaaaa
     2641 atcgccctcg cgtccgccag taaaaacggc ccgttcttgc tcgaacgcat ggggctgacc
     2701 cacttcttcg acaccgtcgc cgaccctgcc gccgtcgcgc attccaaacc ctcccccgac
     2761 atcttccttg tcgccgccga gggcgtgggt gcggacatac gccgctgccg gcgtcgccgc
     2821 catcaaagcc tctggcgctt tgcccatcgg cgtgggcaaa accgaagact tgggcagcga
     2881 catcgcgctg gtgtccgaca ccgccgagct gacctacgcc tacttgcaaa atgtgtggga
     2941 acagtcgggc agataaaatt ctaaggaagt aaaaaggccg tctgaacagt gtttcagacg
     3001 gcctttctgc ttttagaaca gaatgataac ccgagacctt tgcaaaaatc cccaaaatcc
     3061 cctaaattcc caccaaggca tttaggggat ttcccatgag caccttcttc cggcaaaccg
     3121 cacaagccat gactgccaaa cacatcggcc gcttcccgct atcggagttg gaccaggtga
     3181 ttgattggca gccgatcgaa caatacctga
//
LOCUS       DAXFHF010000107         3149 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXFHF010000107 DAXFHF010000000
VERSION     DAXFHF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.107"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            645..863
                     /locus_tag="ACFIRM_001996"
     CDS             645..863
                     /locus_tag="ACFIRM_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029538.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(880..1239)
                     /locus_tag="ACFIRM_001997"
     CDS             complement(880..1239)
                     /locus_tag="ACFIRM_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029539.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1240..1779)
                     /locus_tag="ACFIRM_001998"
     CDS             complement(1240..1779)
                     /locus_tag="ACFIRM_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC3029540.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1939..2643)
                     /locus_tag="ACFIRM_001999"
     CDS             complement(1939..2643)
                     /locus_tag="ACFIRM_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3029541.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            complement(2954..>3149)
                     /locus_tag="ACFIRM_002000"
     CDS             complement(2954..>3149)
                     /locus_tag="ACFIRM_002000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029542.1"
                     /translation="PLAAAFSIAFLISRLSFFDRRFLSCFFIFFLSGKLRMTSASIRL
                     YQLTSDPTGFLFGTNPELRN"
BASE COUNT          777 a          691 c          805 g          876 t
ORIGIN      
        1 ctgccgtaag ccgccgtgcc gtaatatttc accgctttat ccttaagttt gaggaaaccg
       61 cccgcagcat tcaccgtttc gccgtgccgt tgccccgctt tgaagttcga tacttcatcg
      121 ctttgtgcta tccccggctt ggcagatata gcttcgggcg gtttgttttc caaagggttg
      181 tttgtttcga tgggtgtagt ttagtaaaca cttaacatct agtcaagtaa aatgtttagt
      241 gattatttaa taaatactga acattttgat tattaaaaga atttattttt gagatttcgc
      301 aggcgcaaaa aaaaccgcct attaaggcgg ttgtttagga aagaatgccc tctgcgcgtg
      361 tagggtattt cgatacttaa gtttattata tcatatgata taataaactc atagattagg
      421 aaatgaagaa accccgtatc agccgatacg gggggcaacc tgaaagagaa aggaggcaat
      481 gtgattaaga aaatcctaat ctgtgtgatt ttgtatttcc ttgcaagctc cccagcgtgg
      541 tagggaaata caaaacaagt gattaacgat taaccatttg cagggcagtt aaggaggctg
      601 cccttcctct tatctttccc atactctact ataaggattt aaaaatggca aattccaaca
      661 gcgggcattc taaaaaattg cgcgcggcaa cggcggcggc ggcgacaaag gcaaaactgg
      721 caagcggcga ataccggcag ttttcagtgc aggggcgtgc cgaagacgtg gagcttatcc
      781 ttgccgccgt ggaaaaagcc gggggcagcc gcgtacaggc tttggcaaag atttgcaggc
      841 ggtatctcga ggggctgtct taaggggggt aagacaaggt tatttgggaa tatcgggcag
      901 gataaaaggc gggtgtctgt caaaaccgat gattttgata aagccgtcgc aaagggtata
      961 gtgaaggata taggcggatg tcaaatcgcc atacctgccg ccgttgtatt tgggaatgcc
     1021 gatgtgatag tgccaaaggt tgtgccgttg ggcaaatcgg actttttcca gccattgagg
     1081 atcgtctgcc ggtacgttgt cgctggattt gttcctgccg ggtaaccctg ataagccgtt
     1141 ctgttggacg tgtgcgataa attccgcaat ttttctgcgg tcttctttgg ggtagttttt
     1201 caacgctctt ttgaagtctt cgccaagcag cacctgcatt tactgctcca accatttttc
     1261 caagtcgtcg gcgccgttga aatgcgggac gctgacaaag ctgtcttcaa cggctgtttt
     1321 gagtttttca agattgaaat tgtaatccgt ctgcggcagc gcatggcgga aatattcccc
     1381 catcagcgca atccctatga cttcgccctg ccgggtctgt atccacggcg cttcctgatg
     1441 ggttttattg cgtaatgccc aagcggtgta gcagccctgc tctttacgga cacggttgag
     1501 gacaaccaac tcttcgtccg catatttgtc cggttcgata tgggccgcag gcaatgggct
     1561 gccgccgtat tttttgtagg tgcggtaaat gcaggggact accggaccgt gctgccagtg
     1621 ttcgatattt tcggcaaaca gggggcggtt aagtatggca agggcgtagc cttgtgcgta
     1681 atacaggagt ttttgaagtt tgagattgga gatttgctcc ccgtcctctt cttcaaaagg
     1741 ggaaaggaag aaatctgcca cgtcgtacgc gttcagcatt ttctgtttcc gttgttattt
     1801 ttctgtatat taaggccgcc ggcccattat ttcaagaaat aatttgtgca aattatccgg
     1861 tatcggcagg ctgtccgcgc cgtcggcata tcgggcggat atatttgaaa aatacttgcc
     1921 ctgccgtttc agacggcatc aatccaacac gctccaccaa aagaccctgc cgataacgga
     1981 caggctgtcc aaaggggcgg tttcgtcggg atagaagccg ctgttgtggc tgcgtatcag
     2041 cacgctgttg ccgggctgcc gtatcaggta tttgacgcgg aacatgccgt cttgggcaaa
     2101 ggcgtatatt ttgccgtcgc gtatggcggt ttcgcccgta tctacggcga ttgccgcgtc
     2161 ttctgcgatt ttttcctcca tgctgtcgcc ggtcagggtg cagcagaaga cgttgtcggg
     2221 gttgatgcct ttgcgcctta acgttgattt gccgaacggc aggcggtagc cgttgtagtc
     2281 ggggatttca tacgcgcccg ccccgccttt gaagcagctc tctttgaggt aggggacgaa
     2341 aacataatca tcgtcgggca gcgggtcgtt actgctccac atcatcgggc ggtggatgtc
     2401 tttgacttcg tgcggcaggt cggggttgat gaggacgggc gttggttcgc tcggaacgga
     2461 atccaagtac agactgccca tcccatattc ttcttcaagc cgtctgctta ccttttcgcc
     2521 aaaagatgct ttaccgctca tcaattgaga taaaaggctt ttgtctttaa ccggcaactt
     2581 tttattttca aagaatcttt ttaaattatc gatacggatg gccttcagat ctctattagt
     2641 cattttcgcg ctccattcag attgggattc aattttattt agtttctgct aaacaaacaa
     2701 atattaaatt ttcttgccta tgcgtttaat atttgctaaa ctcattaaat atttacggtg
     2761 aaactacaga atgactcatt cagaatttat taaaacgctg tcagaacagc gtggtgcaaa
     2821 aacggaatac gctaaaaaac ttggcttatc cctgtctttt ctatggcaga tagaaaacgg
     2881 acgggcagta acgccaaagc gtctttataa agacgttatg ttgttgacaa aaaataaggt
     2941 ttctatttct gaattaattt cggagttcag ggtttgtccc aaataaaaag cccgtcgggt
     3001 cagatgttaa ttgatataac cgaattgaag cggaagtcat ccgcaattta ccggaaagga
     3061 aaaaaatgaa gaagcaggac agaaaccgcc tgtcgaagaa agacagacgg ctgattaaaa
     3121 aggcgatgct gaaagccgcc gccaaaggc
//
LOCUS       DAXFHF010000108         3146 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXFHF010000108 DAXFHF010000000
VERSION     DAXFHF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.108"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..3051
                     /gene="pilC"
                     /locus_tag="ACFIRM_002001"
     CDS             <1..3051
                     /gene="pilC"
                     /locus_tag="ACFIRM_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC3029543.1"
                     /translation="REYAIVMNKRNQPEVKWTGQYQKSQLREKDGERKFIYTNQKNRW
                     GQQNNFISFDNTDTLVSRQSGTAVFGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTT
                     RAGLSGYSYTGVICRSGKCPQLVYTTRFSFDNPDLLKTGGGLDRHTESSRENSPIYKL
                     KDHPWLGVSFNLGAEGTDKDGRSSNKLVSSFDENNSNSNQNLVYTTEGHRISLGNWQR
                     ETTAMAYYLNAKLHLLNKKQIENIAPGKTVRLGTLRPRVETTGRSWLDFWATWKIEDK
                     GNITVRLDLPEVKAGRCTNTAHPNKTAQALSPALTAPALWFAVQNGKVQMYSASVSTY
                     PGSSSSRIFLQELKTKTDPGRPGRHSLAALNTQDIKSREPNFNSRQTVIRLPGGVYKI
                     NPGKNGGRVAGINGNDGNNGTFGIYKDRLVTPEVDEWSEVLLPWTARYYGNDDIFKTF
                     NQPNNKKQSDKKQYSQKYRIRTKEDDNDKPRNLGDIVNSPIVAVGGYLATAANDGMVH
                     IFKKNGTDQRGYELKLSYIPGTMERKDIENQDSDLAKELRTFAEKGYVGDRYGVDGGF
                     VLRSITDDQDRQKHFFMFGAMGLGGRGAYALDLTKIDSSNPAGVSMFDVQNDKNNNNN
                     KNDSNRVKLGYTVGTPQIGKTRNGKYAAFLASGYAAKQIANQENKTALYVYDLKNTLG
                     TPIAKIEVQGGKGGLSSPTLVDKDLDGIVDIAYAGDRGGNMYRFDLSDNDPNKWTVRT
                     IFEGGKPITSAPAVSRLADKRVVIFGTGSDLSEEDVVGTNPQYIYGIFDDDKGTGTVK
                     VTVQNGTGGGLLEQTLTQENKTLFLSNNKASGGSADKGWVVKLREGERVTVKPIVVLR
                     TAFVTIRKYTGNDKCGAETAILGINTADGGALTPRSARPIVPDHNSVAQYSGHKKTAD
                     DKSVPIGCMWKNGKTVCPNGYVYDKPVNVRYLDEKKTDDFPVTADGDAGGSGTFKEGK
                     KPARNNRCFSGKGVRTLLMNDLDSLDITGPMCGIKRLSWREVFF"
BASE COUNT          890 a          835 c          816 g          605 t
ORIGIN      
        1 cgtgaatacg ctattgtcat gaacaaacga aatcagccgg aggtaaagtg gactgggcaa
       61 tatcaaaaat cacaattaag ggaaaaagac ggggaacgca aatttatcta tacgaaccag
      121 aaaaaccggt ggggccaaca aaacaatttt atctcattcg acaataccga tacccttgtt
      181 tcccgacaaa gcggtactgc cgtttttggc acagccacct acctgccgcc ctacggcaag
      241 gtttccggct ttgataccgc cgagctgaac aagcgcggca atgccgtcaa ttggattcat
      301 accacccggg ccgggctgtc aggctactcc tacaccggcg tcatatgcag aagcggcaaa
      361 tgcccccaac ttgtctatac aacccgattt tccttcgata atcccgactt gctaaaaaca
      421 ggaggcgggc tggataggca cacagagtca agccgcgaaa attcgcccat ttacaaattg
      481 aaagatcatc catggttggg cgtgtctttc aatttgggcg ccgagggtac cgacaaagat
      541 ggcagatcat ccaacaaatt ggtatcttct tttgatgaaa acaatagtaa tagtaatcaa
      601 aacctcgtct atacgacaga aggccaccgt atttcccttg gcaactggca gcgcgaaacg
      661 accgccatgg cctattatct gaacgccaaa ctgcacctgc tgaataaaaa acagattgaa
      721 aatatcgcgc caggcaaaac agtgcggttg ggtaccttga gaccgcgcgt cgagacgaca
      781 ggacgaagct ggctagattt ttgggctacg tggaagattg aagataaagg gaacattaca
      841 gtccgcctcg acctgccgga agtcaaagca ggccgctgca ccaacaccgc ccaccccaat
      901 aaaacagccc aagccctttc gccggcactg accgcccccg cgctgtggtt cgctgtgcaa
      961 aatggcaagg tgcagatgta ttccgcttcg gtttctacct accccggcag ttcgagcagc
     1021 cgcatcttcc tccaagagct gaaaactaaa accgaccccg gcaggcccgg ccggcattcc
     1081 ctcgccgctt tgaatacgca ggatatcaaa tcccgcgagc cgaatttcaa ctcaaggcag
     1141 accgtcatcc gattgccggg cggcgtgtac aagatcaacc cgggcaagaa cggcggccgg
     1201 gtcgcgggta ttaatggcaa tgacggcaac aacggcactt tcggcatcta caaggacagg
     1261 ctcgtcacac ctgaggtcga cgagtggagc gaagtgctgc tgccttggac ggcccggtat
     1321 tacggtaatg acgatatatt taaaacattc aaccaaccaa acaacaaaaa acaaagcgac
     1381 aaaaaacaat acagccaaaa ataccgcatc cgcacaaaag aagatgacaa tgacaaaccc
     1441 cgcaatttgg gcgacatcgt caacagcccg attgtcgcgg tcggcgggta tttggcaacc
     1501 gccgcgaacg acgggatggt gcatatcttc aaaaaaaacg gcacagacca acgaggctac
     1561 gaactgaagc tcagctacat ccccggtacg atggagcgta aggatattga aaaccaagac
     1621 tccgacctcg ccaaagagct gcgcaccttt gccgaaaaag gctatgtggg cgaccgctac
     1681 ggcgtggacg gcggctttgt cttgcgcagc attacagatg accaagacag gcaaaaacat
     1741 ttctttatgt tcggcgcaat gggcctgggc ggcagaggcg catacgcctt ggatttgacc
     1801 aaaatcgaca gcagcaaccc ggccggcgtt tccatgtttg atgtccaaaa cgacaaaaat
     1861 aacaataaca ataagaatga cagtaatcgc gtgaaattag gctacaccgt cggcacgccg
     1921 caaatcggca aaacccgcaa cggcaaatac gccgccttcc tcgcctccgg ttatgcggct
     1981 aaacaaattg ccaaccaaga aaataaaacc gcgctgtatg tatatgattt gaaaaacacc
     2041 ttaggtacgc cgattgcaaa aatcgaagtg cagggcggca agggcgggct ttcgtccccc
     2101 acgctggtgg ataaagattt ggacggcatt gtcgatatcg cctatgccgg cgaccggggc
     2161 gggaatatgt accgctttga tttgagcgac aacgacccga acaaatggac tgtacgcact
     2221 attttcgaag gcgggaagcc gattacctcc gcgcccgccg tttcccgact ggcagacaaa
     2281 cgcgtggtca tcttcggcac gggcagcgat ttgagtgaag aggatgtagt cggtacgaat
     2341 ccacaatata tttacggtat ctttgacgac gataagggga cggggacggt taaggtaacg
     2401 gtacaaaacg gcacgggagg cgggctgctc gagcaaacgc ttacgcagga aaataaaacc
     2461 ttgttcctga gcaataataa ggcatccggc ggatcggccg ataaaggctg ggtagtgaaa
     2521 ttgagggaag gagaacgcgt taccgtcaaa ccgattgtgg tattgcgtac cgccttcgta
     2581 accatccgga aatatacggg taatgacaaa tgcggcgcgg aaaccgccat tttgggcatc
     2641 aataccgccg acggcggcgc attgactccg agaagcgcgc gcccgattgt gccggatcac
     2701 aattcggttg cgcaatattc cggccataag aaaaccgccg acgacaagtc cgtccccata
     2761 ggctgcatgt ggaaaaacgg caaaaccgtc tgcccgaacg gatatgttta cgacaagccg
     2821 gttaatgtgc gttacctgga cgaaaagaaa acagacgatt tccccgtcac ggcagacggt
     2881 gatgcaggcg gcagcggaac attcaaagag ggtaaaaaac ccgcccgcaa taaccggtgc
     2941 ttctccggaa aaggtgtgcg caccctgctg atgaacgatt tggacagctt ggatattacc
     3001 ggcccgatgt gcggtatcaa acgcttaagc tggcgcgaag tcttcttctg acccgcctgc
     3061 gcggccggtt tttccgcaaa tgccgtccga aaggtcttcg gacggcattt tttttgcgtt
     3121 tttcgggagg gggcggcaaa tgaaac
//
LOCUS       DAXFHF010000109         3105 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXFHF010000109 DAXFHF010000000
VERSION     DAXFHF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.109"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            33..1703
                     /locus_tag="ACFIRM_002002"
     CDS             33..1703
                     /locus_tag="ACFIRM_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC3029544.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYLDKFGNE
                     WTKGPSRTKGQEFEWDVQLSKTGREQLGWASRDGKHLNISIDGKITHK"
     gene            1700..1942
                     /gene="imm31"
                     /locus_tag="ACFIRM_002003"
     CDS             1700..1942
                     /gene="imm31"
                     /locus_tag="ACFIRM_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC3029545.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2020..2265
                     /locus_tag="ACFIRM_002004"
     CDS             2020..2265
                     /locus_tag="ACFIRM_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3029546.1"
                     /translation="MKDSTGQMRLATKDLAEAIRKGQVRKSSFNTEQLRAIEKGESKI
                     PDYTWHHHQDTGRMQLIREGLHHDTGHIGWEAMNKGR"
     gene            2268..2658
                     /locus_tag="ACFIRM_002005"
                     /pseudo
     CDS             2268..2658
                     /locus_tag="ACFIRM_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2758..>3105
                     /locus_tag="ACFIRM_002006"
     CDS             2758..>3105
                     /locus_tag="ACFIRM_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029547.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQK"
BASE COUNT          990 a          645 c          686 g          784 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 atccctttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggag cttgccgagc gcagcggcca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgacgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtca tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tatggcagcc atccccatca aagggattgg agctgtccgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctatca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gaaagaatgt gaaactggca aacaaacgcc
     1261 acccgaagac caaagtgccg tttgacggta aagggtttcc gaattttgaa aaagacgtaa
     1321 aatacgatac gagaattaat accgctgtac cacaagtgaa tcctatagat gaacccgtct
     1381 ttaatcctaa aggttctgtc ggatcggctc attcttggtc tataactgcc agaattcaat
     1441 acgcaaaatt accaaggcaa ggtagaatca gatatatccc acctaaaaat tactctcctt
     1501 cagcaccgct accaaaagga cctaataatg gatatttgga taaatttggt aatgaatgga
     1561 ctaaaggtcc atcaagaact aaaggtcaag aatttgaatg ggatgttcaa ttgtctaaaa
     1621 caggaagaga gcaacttgga tgggctagta gggatggtaa gcatttaaat atatcaattg
     1681 atggaaagat tacacacaaa tgactaattt aaaattagat ttctactctg aagttattat
     1741 aaaagattct tgccctaatg atttgttaga aaatggtgag actattaaag gaaaaaaagg
     1801 agtggtatta ggtataagcg aagaagatgg tataatctat ggctatacta ttttactttt
     1861 tgatataaaa tattgtatat atatagataa gaaatatatt ataccgactg gaaaaaaatt
     1921 ctctcgcgat gatttttatt agctaattta agtaatttaa gcaagttttt tagatactga
     1981 aactggtaat tatgtttatg taagcataag gagttcagat tgaaggattc tacagggcaa
     2041 atgaggctgg caaccaagga tttggcggaa gccattagga aaggacaggt tcgcaaatca
     2101 agctttaaca cagaacaatt aagggcaatt gaaaaaggag aatctaaaat accagattac
     2161 acttggcatc atcatcaaga tacaggaagg atgcaattga ttcgtgaagg cttgcatcat
     2221 gataccggcc atattggttg ggaagcaatg aacaaaggaa ggtaactatg tggaaaatca
     2281 taaaagagga tagtgatgat ttagaatttg caattaaatg cttattctct caatctattg
     2341 atttaaatga attcaagtta tggattgaac aagtaatacg cgatatgccc atcgaggaca
     2401 tcccttttta tatttttgac ttggcggatt tcaatggggg aattggagac attgacaata
     2461 ttgtaggttt tgtttcgagt tacagcctat caaaatcgaa aaaaaatgcc ttgacaggca
     2521 ttgccttctt aagggggata gatgtctata gaccctcccg tttcaaaaga aaaagcatta
     2581 aaagccttag agaaatatcc tgaaatttat cagaaatttc agcatttctt tccgtttgta
     2641 gaacttccgc ttctttaaag gacaatatgc cgtctgaaac gatattcggc tttcagacgg
     2701 tatttttgat ataaagcggg taactaaaag agcgtttgac ggcaaaggaa gataattatg
     2761 tggaaaatta cgaaagagca aggtcaagat ttaagttttg caacatattg cttattatcg
     2821 gcagcaatta atttgcaaga atttaaatta tggttggaga aggttgtctt agatatgcct
     2881 attgataata taccttttta tatttttgat ttaattgatc ttaaagaagg agtaggagat
     2941 atttataata ttttggattt tgttccgaat tcagacttat ctaaagacca agatgacgca
     3001 ttaacgggca ttgccttctt aagggggata gatgtctatg accctcccgt ttcaaaagaa
     3061 aaagcattaa aagccttaaa gaaacatcct gaaatttatc agaag
//
LOCUS       DAXFHF010000110         2986 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXFHF010000110 DAXFHF010000000
VERSION     DAXFHF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.110"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..235)
                     /locus_tag="ACFIRM_002007"
     CDS             complement(<1..235)
                     /locus_tag="ACFIRM_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC3029548.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTE"
     gene            complement(291..515)
                     /locus_tag="ACFIRM_002008"
     CDS             complement(291..515)
                     /locus_tag="ACFIRM_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029549.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAATAMIAAHFIFVALSD"
     gene            complement(940..1226)
                     /locus_tag="ACFIRM_002009"
                     /pseudo
     CDS             complement(940..1226)
                     /locus_tag="ACFIRM_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1462..1734)
                     /locus_tag="ACFIRM_002010"
     CDS             complement(1462..1734)
                     /locus_tag="ACFIRM_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029550.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2611..2862)
                     /locus_tag="ACFIRM_002011"
     CDS             complement(2611..2862)
                     /locus_tag="ACFIRM_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029551.1"
                     /translation="MNIKNEIVALISKKIDGGGQYSSLENFALSVLREKIINNTVSSD
                     EKLLIINDFLSGFLEFDPETGEPVGETLKIEQMIDFFLV"
BASE COUNT          940 a          605 c          480 g          961 t
ORIGIN      
        1 cttccgtcag cttttgcccg taaaaattgt tcaaaagcgt cgccaccgaa gccgccccgc
       61 aggaaaaatc caaatcctgc tttacaatat tgaaatcccg ccgcgctttc cagctttgta
      121 ttttgacttt gccgtaggct atggggtttt cttccgcaaa gggaaaagaa tgtgcacaaa
      181 gcggcagggc ggcggcgagg agcagggaag cggcaaaccg ccttttttgt tccatcaaaa
      241 tcctcctgaa aggctgcctg aaatttccaa gcagcctttt tgtttgctta ttaatcggat
      301 agtgcgacaa atataaagtg ggcggcaatc attgccgtag cggcagccat aactgtttta
      361 ttttcttcaa accaaagcaa gttgagaaac ataaacatca agaaatatga tggttcttca
      421 gttttgccgc tgctttcttg agttagaaga taaaatatgg atatggcggt tatgccgatt
      481 gctgctttta ttaaaatata tttgttcttc atcatcttgt atggttttta atgcaagttt
      541 caacaataaa attcggaata agatagagag atgttgatat atcggataaa tatatagtgg
      601 attaaattta aaccggtacg gcattacctc gccttagctc aaagagaacg attctctaag
      661 gtgctgaagc accgagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc
      721 ttgtcctgat ttaaatttac tccactatac aaaacaatca agaaacgatt ggcggtatgt
      781 ataatcttga attttgtttt ttgatgaaat taaaataatg aaatatatgg taaacgttac
      841 aacggaagcc ttagcctccc gattgcagta tttcaattcc aagatggatg ccgcatagaa
      901 gataaaatac tcaaatggtt ttttagcggc ttaaatatct tatttaatct tattgattgt
      961 ttttccaatg gtttctgcaa tcaataataa gaaatagaat aataaacaaa cagaacatga
     1021 aaataaaaat aaatctttca ttatcccagt aataaaattc caaagatttt ccttatttcc
     1081 atcaacaata aaaattatca gttcatgaat aaagaatact gcaaagaaaa tgcttcctgc
     1141 cgttagggat atttctaacc tgcttatttt cccttcactg tatttacctg ataaatagcc
     1201 taaaaagctg caatcaaatc atctatttta aaaatttctg taaaaacagg taatgccttt
     1261 aattcattac ctgttttgcc attatttatc tatgatataa gcgtctctta cccttcagcc
     1321 tctttgggaa atcaaagcct gcttagaacg tatgttgcac acccaatttc aataggtaaa
     1381 tcagattgca aatccagcaa tttgaatatt gtcattgttc cgtgcaaaag ggatctttat
     1441 tgatgtgttg tgtactgggt ttcagcttgg ctttttagat aatcttttca caagaacata
     1501 catcaatact gtccaaaaaa aaagatttga taaatatatt gcaacatatt ttattccacc
     1561 tccatccgaa acagaaacca atatattttt aattaataca tagctgatta ttacatttat
     1621 tattcctata atatataagg atcttgccaa aatttttttt gattttattt taggaatatc
     1681 tcttatccat gctaaaatac agcccaatgt cgaaaagaaa ataacagcaa acactacctt
     1741 acctcctctc acttccaacc aaaccgatag caggtttggt tggaagttgg tttatttatt
     1801 atttaacggc gaatgtcagt gttcttaccc gtagaacctg cataaccagt tatgccacca
     1861 gcaacagccc ccactgctac acctcgcata ccattaatca gtgctcttcc tattttttcg
     1921 aatccactat ttcttccaaa gatgcagcaa acgcaggttg agcctagacc caatctacgt
     1981 ttgctgccgc atctgatccc tattgtttct tatccttaca tcttcctgcc ttgtcaacca
     2041 aataaagaca gaagactata caaaaactga ccgacatact gaaaatacct atccccttcc
     2101 atgcaatctc cgcaccattg acccaataga tcagactgag aaacaacaaa gccacagtat
     2161 aaatcaacgt aaaaaatata gcgtaaagcg caataaaggg aacttttatc tcacggttgt
     2221 tttttataat atattcagca acttgatttc cgaatatacc tgataaaaaa tatagtatta
     2281 gatcatccat ttcgtttatc ttctatgttt tcccattgcg gcgctagaag actgatttaa
     2341 tgcatgcacc atgcaatata gtggattaac aaaaaccggt acggcgttgc ctcgccttag
     2401 ctcaaagaga acgattccct aaggtgctga agcaccaagt gaatcggttc cgtactattt
     2461 gtactgtctg cggcttcgtt gccttgtcct gatttttgtt aatccactat agtttaaaca
     2521 actttatttt tgattttatc ccaagtataa acctgacaat cgtatttaaa agccggatct
     2581 acgcctagct tgtccactgg tatgtatgat ttagactaaa aagaaatcaa tcatttgctc
     2641 aattttaagc gtctccccga caggttcccc tgtctcagga tcaaactcaa gaaaacctga
     2701 taaaaaatca ttaataatca ataatttttc atcactggaa acagtattgt ttataatttt
     2761 ttcccttagt acggataaag caaaattttc taaagaacta tattgccccc ccccatcaat
     2821 ttttttagaa attaaagcaa ctatttcatt tttaatattc ataatttatc cttaagttat
     2881 aaaaggggca aagccccctt tttttgatta acgaggcttt gttgcgcgat ttttcgtaaa
     2941 ccgtgctttc aagaaagctg attagggcgg ggtttttgcc gtcttt
//
LOCUS       DAXFHF010000111         2873 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXFHF010000111 DAXFHF010000000
VERSION     DAXFHF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.111"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(152..541)
                     /locus_tag="ACFIRM_002012"
     CDS             complement(152..541)
                     /locus_tag="ACFIRM_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC3029552.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIIEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQTAR"
     gene            complement(704..1083)
                     /locus_tag="ACFIRM_002013"
                     /pseudo
     CDS             complement(704..1083)
                     /locus_tag="ACFIRM_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1137..2270)
                     /gene="carA"
                     /locus_tag="ACFIRM_002014"
     CDS             complement(1137..2270)
                     /gene="carA"
                     /locus_tag="ACFIRM_002014"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC3029553.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          612 a          709 c          803 g          749 t
ORIGIN      
        1 gagaggacgg gtcggctgtt ggggttgagg tttgctgcaa ggaaaacagg ttgtgcaggt
       61 tgctttttat ttttcagacg acctttttaa acgcttaatt cagttaattt taccttattt
      121 ttttcaaaat ctaatcaaaa tcaaccgttt gctaccgtgc tgtctgcttt tccaattcct
      181 gcaatacgcg caggatttcc gccagtacat cgtttgtctg ctgcaaaatt tcgtgattcc
      241 aaaaacgcag cacggtaaag cccaagctgt tgagatatac cgtccgcgcg tggtcgtata
      301 cggcttgttc cgcgtgctgc ccgccgtctg cttcgataat cagtttgggc gttacgcaca
      361 taaaatcaac aatataattc cccatcggct gctgtcggcg gaatttatag ccgttcagac
      421 ggcctgcccg caggtgttgc cacaattttg cttccgcctc gctcatttct tgacgcatgg
      481 ctttggcgcg ttggcgtagg gcggggtttt cggcggtcaa tagtttctcg ggcgggttca
      541 tttttgttat cgggttgctg tttgtgtaag ccttacagct tgttgccctc tctctaactc
      601 tttcccacag ggagagagga cgggtcggct gttggggtta aaggggctgc aaggaaaaca
      661 gcttgtgcag gctgtttttt attttgcata atccgtctga aaattaataa ctgctgattt
      721 ccagcaggtt gccatcggga tcgcgcaggt aaaccgattg gattttgccc attgcgcctg
      781 tgcgcgctac gatgccgctt aaaggtttga tgccgtgtgc ggataattcc tgtaaaaccg
      841 tttccagtgg ggcgtatcgg tcagcaggca taaatccgct gtgccgcagg cggcgtgttg
      901 cgcgttaggc tgaatttccg caccgcgccc gtgtaggttg attttctgac tgccaaacaa
      961 caaagcttta cggttgttgc caaatgaaac ttcttccatg cccaaaactt gtgtgtaaaa
     1021 cgcgatggtt cggtcaatgt cggcaacagt cagtactaga tggtcgagtg cgctaatttt
     1081 catttccagc tttctttttc agacggcagc agcatactgt tgccgtctga aaatcattat
     1141 gcttgttttg cagccttcat attgccaatg aatttgtcga acaaatagcc gacatcttgc
     1201 ggacccgggc tggcttcggg gtgtccttgg aaacagaaca cgggtttgtc ggtcagctcg
     1261 atgccttgca aggtattgtc gaacaaggat ttgtgggtaa tgcgtgcgtt ggcgggcagg
     1321 gtgtcggcat cgacggcgaa accgtggttt tggctggtaa tgacgacttt gccgctgtcc
     1381 aaatcttgca caggatggtt cgcaccgtgg tggctgaagc gcattttcag ggttttcgcg
     1441 ccgatggcga ggctgatgag ctggtgtccc aagcagatgc cgaaaatcgg tttgccgctt
     1501 tccatcagtt tttgcacggc ttcgatggcg taggtgcaag gctcggggtc gccggggccg
     1561 ttggacagga acacgccgtc gggattgagt gccaacacgt cttccgcgct cgtttgtgcg
     1621 gggacgacgg tcaggcggca gccgcgcgag gcgagcatac gcaggatgtt ggttttcacg
     1681 ccgaaatcgt aggcgacgac gtggtaaggc tgtttgtcag gggtaacgaa acctttgccc
     1741 aattcccatt cgccttccgt ccattcgtaa gtttccgtgc aggaaacttc ttttgccaaa
     1801 tctttgccga ccatgctgcc gaacgcggcg atgagttctt gcgctttttc aacggtggca
     1861 tccgcacctg tcagaatcgc gccgccttgc gcgccttttt cgcgcaacag catggtcagg
     1921 cggcgggtgt cgatgtcggc gatggcgacg gtttcgttgc gtaccaaata gtcgtgcagg
     1981 ctttcggagg cgcggaagtt gctgtgcaag agcggcaggt cgcggataat caggccggcg
     2041 gcataaacgc tgcggctttc ttcatcttcg gcgttggtgc cggtgttgcc gatgtggggg
     2101 taggtgaggg taacgatttg tttgcagtag gacgggtcgg tcagaatttc ctgatagccg
     2161 gtcatcgaag tattgaacac gacttcgccg gaagccgaac cttcgtaacc gattgatgtg
     2221 ccgtgaaata cgctgccgtc agcgaggacg aggagggcgg gggtgctcat gatgggaatc
     2281 ctgtttttaa taaaattctt gacaatgccg tctgaaggga cttcggcagg gggcatccgg
     2341 tcggaaaaat ccggtcaaaa aaaaacacgc cgcgcaagat agacgcgtaa cgtgcttttc
     2401 gggcaagtgc ctataccctg aaagcagcgt attctaagac gaaacctaca cgatttcaag
     2461 cgcaaacgcg gcaggcgggc tgatatgttc gcgcgaaaac ggaaatccgg ggcataaaac
     2521 cgcccgctcc gcgcccccta tgcccgttac gcatttcccc acgtccgccc ggcgaaacta
     2581 tgggaatacc cgaaccgtca ttcccgcgca ggcgggaatc cggacctgtc ggcacggaaa
     2641 cttatcgggt acaacggttt cttgagattt tacgtcctag attcccgcct gcgcgggaat
     2701 gacggcggag cggtttctgt tttttccgat aaattcctaa aactcaaaat ttcatcattc
     2761 ctacaaaaac agaaaaccaa aaacagaaac ctaaaatccc gtcattcccg cgaaagcggg
     2821 aatccagaat ctctaaagct tcagctaacc tttgaatatt gctgttgtcc cac
//
LOCUS       DAXFHF010000112         2851 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXFHF010000112 DAXFHF010000000
VERSION     DAXFHF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.112"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            69..1193
                     /locus_tag="ACFIRM_002015"
     CDS             69..1193
                     /locus_tag="ACFIRM_002015"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3029554.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1190..2227
                     /locus_tag="ACFIRM_002016"
     CDS             1190..2227
                     /locus_tag="ACFIRM_002016"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC3029555.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDIISQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2228..>2851
                     /locus_tag="ACFIRM_002017"
     CDS             2228..>2851
                     /locus_tag="ACFIRM_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3029556.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQP"
BASE COUNT          915 a          510 c          594 g          832 t
ORIGIN      
        1 agagcatagt tcacctttaa aatccataca aggattttta tcaaatttat gcataggagt
       61 gaaatcctat gctatctaaa caaatctcaa atcttaattc ttctagtaac aaaccaaaaa
      121 tcctatctct attttcagga tgtggcggtt tggatttggg ctttcatcaa gctggttgtg
      181 aaactgtttg ggcgaacgat ttctcccatt gggcttgcga aagtttccgt aaaaatatcg
      241 gcgatgtcat cgtagaaggt gatattgaac aaattaatcc gaatgatcca actattcccg
      301 attgcgacat cattttaggc ggattccctt gtcaagattt ttccatgatt tggaaacagc
      361 cgggcttaga gggtgagcgc ggcaatcttt ataaaagctt tttacgtttt gtaaatgcaa
      421 aaaaaccgaa agtttttgtt gctgagaatg tgaaaggttt attgactgcc aacaagaaaa
      481 aagccatcca gcaaattatt accgactttg aaaattgcgg ttattacgtt caggcgaagc
      541 tgtataactt tgcagaattt ggcgtacctc aatttcgtga acgtgtgctg attgtcggag
      601 tacgtttgga tacaggattt gattttcgcc atccggaacc gacgcacaat gaaactggcg
      661 aaaacggctt aaaaccatat gtaacagcag gtcaggccat atccaatatt ccacaaaatg
      721 ccagtaataa tgaattactg aaaatcagcg ataaaacacg ccgtatgttg gaattaattc
      781 ctgaaggtgg aaattttacc gatattccta aagatcatcc tttatatgtg aaaggtatga
      841 ttagccacgt ttatcgtcgt atgcatcgga acgagccatc aaaaacaatt attgcagcag
      901 gtggcggtgg tacttggggc tatcacttcc ctgaaccgcg tgcttttact aatagagaac
      961 gagcaaggct tcaaagtttt cctgatgatt ttgagtttgt cggatcaaca actgaagtac
     1021 gtcgccagat tggtaatgct gttcctcctc agggcgtggt tgaactggca aaaagcattt
     1081 taccgatttt ttcagacaac tatgagaaag tagatttgca tgagaaatta gtcgaagaaa
     1141 aagaaatttt attccatgac cgactaagca aaattcgagg gggaaaacaa tgaatacagt
     1201 tttttccaat atcgccaatg ccaaaatcac tgaaaaatct cttaatgcgg tttggatgga
     1261 tttatttaaa tcagcggatg aagtgttgat ggcgacaggt tatgtatcta atgacgcagt
     1321 ggttgagtta cataaaattt tggagctaaa cgatcatatt caaaaaatag atttattggt
     1381 cggaatgcat tatttggaag gttttagcca tttacaatat gacagcttat gtaaactaaa
     1441 tgattttttg cgacatgaga aaagaggagc cgtctatgtt tccccctttg tgaaatttca
     1501 cggcaaaatg tattccttta aaaattatca aaaaatcaat ggattaattg gttctgctaa
     1561 cttaacgtgt ttttgggata gcacggaacg tacttatgaa actatgttgc atttgaacgg
     1621 taagcctgca cagattttgc aggcagatat tcaaagtact attcataagc taggcaaaaa
     1681 tatccaagaa gttgaaagac caagtaaatt tattgaacat aatagccatt tagaaaattg
     1741 tttgggtgtt cagaaaattg caccagaaca gattcggcaa ttatttgccc aaacgtcaga
     1801 atatcatttc tcaattccag ctaaaaccga agaaaaaagt aatttgaatg tattttttgg
     1861 agaaggtcga cgagataaac gagggtttgt caaaccacga ccttggtatg aagtagagtt
     1921 gattgtatcc aaagacatca tttcacaaga agggtatccg gttctgaagt cttttacagt
     1981 tattaccgat gatggttggc aatttcaatg caagacatcg ggggattact ccaaaaactt
     2041 ccgctcagaa aatgatttaa agacacttgg taaatggatt aaaggtcgtt tggaaagtca
     2101 tggctgcctg caaaataacg agaagattac gcatgaaacc ctacgcgaat acggtaacga
     2161 tcattttgag ttacgttcaa ccgataatcc tgatgtttgg ctgctctcat ttaaggggaa
     2221 aaactaaatg ttgcgaacct acttgaacca attgacccct cccgaattag ctgattctgt
     2281 taaaaatacc gttgatggtt ttatggaaaa attgtcacaa accgagccga aaatcgccca
     2341 aaatgtttta ttactgggca atgttcaaag tggcaaaacg gcacaggttt tgggtgtatt
     2401 aagtgcgctg gctgatgatg gcgaccacaa agtttttttg tatcttacta ctgacagcgt
     2461 agatttacag gatcaaacag tcaaacgagc caaagccaat ctgaaaaact ttatcgtatt
     2521 gtctgaagct gacgaccgaa gttttatgga agtaatgaag gcagagaacc ccatcttggt
     2581 cgtgattaaa aagaatgccc gtgtattgaa acgttggcgt aatttgtttg ccagtcaaag
     2641 tagcctgaaa ggctatcctt tggttattgt ggacgatgaa gccgatgctg ccagcctaaa
     2701 tactaattct gataagccgg ctaaagatgc cagtactatc aataaactgc taaacgacat
     2761 caaaaattcc tgttgtcaaa gcctgtttat ccagttgact gccacgcctc aatcgctttt
     2821 attacaacat gaagaatctg attggcaacc t
//
LOCUS       DAXFHF010000113         2654 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXFHF010000113 DAXFHF010000000
VERSION     DAXFHF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.113"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            87..536
                     /locus_tag="ACFIRM_002018"
     CDS             87..536
                     /locus_tag="ACFIRM_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3029557.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(913..1059)
                     /locus_tag="ACFIRM_002019"
     CDS             complement(913..1059)
                     /locus_tag="ACFIRM_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029558.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            1446..2036
                     /locus_tag="ACFIRM_002020"
     CDS             1446..2036
                     /locus_tag="ACFIRM_002020"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3029559.1"
                     /translation="MDYELSCKETYEANINAKFIQADIFELQPETLEKELGLKKNDDD
                     LILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGILSRMKE
                     SGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSSIRANGL
                     RYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
     gene            2075..2404
                     /locus_tag="ACFIRM_002021"
     CDS             2075..2404
                     /locus_tag="ACFIRM_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3029560.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            2418..>2654
                     /locus_tag="ACFIRM_002022"
     CDS             2418..>2654
                     /locus_tag="ACFIRM_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3029561.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIG"
BASE COUNT          828 a          409 c          630 g          787 t
ORIGIN      
        1 gtgggaaata tgcatactgc tgaatgggat agtaagtctg ttcaaagaaa tatgttgaat
       61 aatctgttct tattggaagt aaagtaatga ctgataatcg ggggtttacg ctggttgaat
      121 taatatcagt ggtcttgata ttgtctgtac ttgctttaat tgtttatccg agctatcgca
      181 attatgttga gaaagcaaag ataaatgcag tgcgggcagc cttgttagaa aatgcacatt
      241 ttatggaaaa gttttatctg cagaatggga gatttaaaca aacatctacc aaatggccaa
      301 gtttgccgat taaagaggca gaaggctttt gtatccgttt gaatggaatc gcgcgcgggg
      361 ctttagacag taaattcatg ttgaaggcgg tagccataga taaagataaa aatcctttta
      421 ttattaagat gaatgaaaat ctagtaacct ttatttgcaa gaagtccgcc agttcgtgta
      481 gtgacgggct ggattatttt aaaggaaatg ataaggactg caagttactt aagtaggctt
      541 gtttacaaat cgatcgatag tgtttcagta tttgtgcggt tttccgcctt gatggaatga
      601 gaatcgtgta atattaagcc tatagttatg acccgtgatc cgtatctgga ttgtttctgg
      661 ataaaagaat aaacccaatt atctttagcc gtggatttat ggtgcatggt agcaaaaatt
      721 gtataggggt gtacattact catctgaaaa ggtgaacaga ttttataatg ccgtctgaag
      781 atgttttcag acggcatttt gtttccaatt cgttataggc ttagtaattt aaggcatgtt
      841 tgcaccttta gacataaggg caatcgcagc cccgtcctta atgtacaaat cactatccag
      901 cttcaaccat gcctaatagt tttccgaatc gaataccttc gcccgataac gccctaagga
      961 aattttttgg acatcgcgaa ccttgtcctg ctgaaaatac aaaaggccgc gttcgacgat
     1021 gtggggcgga aataggaggg gtaggtagat acgggacatg gtttgcttcc aaggagttgt
     1081 tgaaatatgg ggaatttact ggtttgggcg atgtttgggg agaggggtgg gaattcttgt
     1141 tttgcgaagt ggaaaaaggt cgtctgaaaa tgggtttcag acgacctttc tgtgaaatgt
     1201 gcatttgaag atatccgatc gtttggagga aattaagttc tgcttattta gatgcatgaa
     1261 ttttaataaa atctcaaatt atttttgata agcaataaat tatatgttat ataaagttag
     1321 ttctcactag tctaattatt taaatagaaa tgaaaaacag taagttaaag gcggttgatt
     1381 ttttctgtgg cggaggcgga atgagctatg gaatgtaatc cgccggtatt caggttttag
     1441 ccgggataga ttacgaactt agctgcaaag aaacatatga agccaatata aatgccaaat
     1501 ttatacaggc tgatattttt gaattgcagc ctgaaacctt ggaaaaagaa ctcggcttaa
     1561 aaaagaatga tgatgatttg attcttatag gttgtagtcc ttgtcaatat tggagtgtta
     1621 ttcaaacaga caaaagaaaa tcggaaaaat ccaagagttt gcttttggag tttcagcggt
     1681 ttgtagaata tttcaatccc ggatatgttg ttgtagagaa cgtaccggga attctgagca
     1741 gaatgaaaga aagcgggctt gataatttca taaagttatt ggaagaaaaa ggatttaccg
     1801 tccatttcgg tattcacaat acggctgatt acggaattcc ccaaagccgt aaaagattta
     1861 cgttaattgc aaacagaata accaaaaaaa gctggaacca gtcaagtatt cgggcaaacg
     1921 gcttacggta cgcgatgttt tgggaatgga aaacggcttt cccaaaatta tggcaggaca
     1981 tcaagacgag acggatttta tgcatagttg tgcgggatta tccgatatca atttgaaacg
     2041 attggctttg atacctaaaa acggaggaaa ccgtttggct tttgcgcata ttcccgaatt
     2101 acagctggaa tgttttatcg gaaaagacaa tagttttaaa gacacgttcg gtaggctatg
     2161 gtgggataag cccgcaccta ccataacgac caaattcttt agcatttcca acgggcggtt
     2221 tgcccatcct gaagaagata gggcgttatc cctgcgagaa ggtgcaacat tgcaatcgtt
     2281 ccctcgtaac tatgtcttta aagcgggcag tagggacaag atagcccgtt tgattggtaa
     2341 tgccgttcct ccgatgtatg cggaaaaaat aggcagggca attgttgata atatcgaatg
     2401 ttagaaaagg agaaaacatg tctgaagaaa aattgaaaat gagtttcgag ccaaccgtaa
     2461 tcgaacattt gggtgtaaag atgtattcgc acactgttcc tgcgattgcc gagttgatag
     2521 cgaatgccta cgatgcatgt gctacggaag tggaagttag gttattcgat aaaccggagc
     2581 ataaaatcgt tattaaagat aatggcatag gaatgagctt cgatgaaatc aatgattttt
     2641 atttgagaat cggt
//
LOCUS       DAXFHF010000114         2535 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXFHF010000114 DAXFHF010000000
VERSION     DAXFHF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.115"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(156..371)
                     /locus_tag="ACFIRM_002023"
     CDS             complement(156..371)
                     /locus_tag="ACFIRM_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029562.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTSN"
     gene            complement(495..854)
                     /locus_tag="ACFIRM_002024"
     CDS             complement(495..854)
                     /locus_tag="ACFIRM_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC3029563.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            complement(851..1603)
                     /locus_tag="ACFIRM_002025"
                     /pseudo
     CDS             complement(851..1603)
                     /locus_tag="ACFIRM_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1843..2049)
                     /locus_tag="ACFIRM_002026"
     CDS             complement(1843..2049)
                     /locus_tag="ACFIRM_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029564.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(2059..>2535)
                     /gene="mafB"
                     /locus_tag="ACFIRM_002027"
     CDS             complement(2059..>2535)
                     /gene="mafB"
                     /locus_tag="ACFIRM_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3029565.1"
                     /translation="AGTVWGGKKVELNPAKWDWVKNTGYKTPAVRTMHTLDGEMAGGN
                     RPPKSITSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFMDLNINSPADFAR
                     HIENIVSHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
BASE COUNT          690 a          521 c          442 g          882 t
ORIGIN      
        1 ccaattttta tcggggtcgt tttcccatat cagttttcct gcttcatcag cacagttagg
       61 attggcattg atgcccaccc agtttcaggc agcctgaaaa ggatttttaa gatacggcta
      121 ggtgggctgc gagaacccac actctgcata accatttagt tagatgtatc cattagatta
      181 tatgcttcaa ttaaatgttg ttctagctct tgcactaatt gaggattgcg aattcctgac
      241 acaatctcaa attcttctgc atctatgcaa acacctgcat aaatttcaat aacaagggaa
      301 cgcaataatt gaagctcttc tcttgttaaa gaaataataa tgtcatcacc tgtgtaattg
      361 attatattca taataatttt atttttgctt gtcaaagtaa gttttaccta aggttggtct
      421 aaatgcagtc ccaccatctt ttgaatttgg gtctctaatt acaattgctc cagacttatc
      481 atcccaaatt gctcttatgt gtttggattg taatcttcga attcccaaga aaaaaatcgt
      541 aataagtttg aaagtgtcaa atcccaagtt tcttttgagc aatattctaa tattttatca
      601 atttcacttt taataatctt atgatcaaac tgttctaata ttaatgcatt agaccaaaaa
      661 aaaccttctt tattacaatg atgggaaatc catttaggag aacaaatgca aagtgaaaaa
      721 atagatgagc cttgttctcc ttcgattccg atatccaaat ctatccatct atgaaaatta
      781 tctggaattt cgggggtaaa tttttcaaaa tcaatatcat ataaatttat gctttttaag
      841 tccaatttaa tcattttata ttcaaatgtc cattagttaa ggtttttcca tcaacacttc
      901 tagtaacaaa attagcttgc actcccgttg ggttaagtga ttcaggggtg tttgattttg
      961 tagttggagg acgatatagc cttgtgccat ctgcacttaa catacatcct tgacaagatg
     1021 gactactaac tggcttagcc ccgtctccaa cccaaatttt tcctaactgt tcagcctctt
     1081 gacgagttgc agaaccaata ccaaaattaa tttccccctt tttatttggt tgtattgttt
     1141 tccaatcttt tacagcactt gctaaccttg aatcttgagc cgcaatgtta tttaagtttt
     1201 gctcatttag ctgattaacc aacttaggat aggttgcagc attagctttt cctttcgacg
     1261 ttatagattt aggcggtcta ttcccaccgg ccatctcccc atccaaagta tgcatggtgc
     1321 gaacagcagg tgttttatag ccggtatttt taacccaatc ccatttggcc gggttaagtt
     1381 ctactttttt accgccccaa accgtacctg cggcctctgc tacggcaagg gcagtttggg
     1441 ctgttgcagt tatattcgga tgggcatcag cccattgtct ggcggaattg atgccgtctt
     1501 ttaccgcaaa ggcactgtct tgtaatgcgg ttgcagccgc aagttgtgct gcgggactta
     1561 aatttcctaa atcgtgaata ccctgtaaag tttgctgcgc agaaatgtag gtcggatatt
     1621 tatttgtatc cgacaaaaat atttgacgcg cctatcgttt ccgagatgcc gctgttggaa
     1681 aatgtcggat tcgagaatcc gatctacggc tgctagtggg tgctccatca ggcatattaa
     1741 caacatcaac cctgtcgcca acccatgatt tttttctttc atttcggcta caatagaatc
     1801 atagttgtaa tttgcactag gctctgacaa aaatttgggt tgttaagttg tcattttttt
     1861 ataaattcca gcaaactgtt gttgtaaatt atctacttca tgttttctaa caccagatac
     1921 attttcaaat tctcttgaat ctacacatac gcccgcatat atctcattca agatagctcg
     1981 taaaacaaaa acttcatctt gagttagtga aatagttgag ttttgattat ttatggatag
     2041 tatattcatg gctttttcct ataaatcatc ataatatttt ttacctgatg ttggtctaaa
     2101 tgctgtacct ccatcgtcag aatttttatc tcggataact attgtccctg ttttatcatc
     2161 ccaatacgca gttctaccgc gaggtaactc tttcatattt gttggatggc taacaatatt
     2221 ttcaatatgc cgagcaaaat ctgctggtga attgatattt aaatccataa attcttgttg
     2281 tcttatgaca tgcttgttat aagcatgccc actactaatt tgttctccaa ttagttgcgc
     2341 ttgtaaagac ggttgtgtgg aagcatctgc tttgctgttg gacgttatag atttaggcgg
     2401 tctattccca ccggccattt ccccatccaa agtatgcatg gtgcgaacag caggtgtttt
     2461 atagccggta tttttaaccc aatcccattt ggccgggtta agttctactt ttttaccgcc
     2521 ccaaaccgta cctgc
//
LOCUS       DAXFHF010000115         2465 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXFHF010000115 DAXFHF010000000
VERSION     DAXFHF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.116"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..205
                     /locus_tag="ACFIRM_002028"
     CDS             <1..205
                     /locus_tag="ACFIRM_002028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029566.1"
                     /translation="AADSTDSTEPIRPVLHHLRESFSLSRGGATPYRFLFIRYSIDKH
                     IIFNIFNWIIVYLSKDNCLFLTN"
     gene            419..931
                     /locus_tag="ACFIRM_002029"
     CDS             419..931
                     /locus_tag="ACFIRM_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC3029567.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            1011..2090
                     /gene="aroB"
                     /locus_tag="ACFIRM_002030"
     CDS             1011..2090
                     /gene="aroB"
                     /locus_tag="ACFIRM_002030"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC3029568.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          588 a          767 c          632 g          478 t
ORIGIN      
        1 agccgcagac agtacggata gtacggaacc gattcgcccg gtgcttcatc accttaggga
       61 atcgttctct ttgagccggg gcggggcaac gccgtaccgg tttttgttca tccgctatag
      121 tattgataaa catattatct tcaatatatt caattggata attgtttacc taagcaaaga
      181 taattgcctt ttcctgacaa attaagtgaa atcaacggat tgtcaaaaca cagcctgaaa
      241 taaaaaacct ccctgatttc ttttattttt ccttaaaatc agaaaagttc gggatggtcg
      301 ggttattttt ccaaacgtac cgccgccctg ccgatttcgt ataaaatccc gccgtaaccc
      361 gacaagcccg aaccctgccg ccccgaaagg cgggggggtc aaacattaag gaaccgtgat
      421 gaaaaacttt aacggcaaac tcatcctcat cggactgatg ggcgcgggca aaactacgct
      481 gggccggcaa atggcgcagc ggctggatta ccgtttttac gacagcgatc acgaaatcgc
      541 cgcagcggcg ggcgttccca tccccaccat atttgaaatg gaaggcgaac agggattccg
      601 ttcgcgcgaa accgccatac tcaaaaaact gattgtcctg ccgcatatcg tcctgtccac
      661 cggcggcggc gcggtgttaa aagaagaaaa ccgcgccctt atccgcaaaa gcggcacggt
      721 cgtttatctg cacgccccgc ccgaaaccct gctcgaacgc acgcgctgcg acaacagccg
      781 ccctttgctg caagttgccg atcctttggc gaaattgcgc gaactctacg ccgcacgcga
      841 ccccgtttac cgccaaaccg ccgactttac cgtagaatcg gcaaactgcc gggaaaccgt
      901 gcaaaccctg ctcaaacgct tatcccgata aaccggcata tgcgccgctc cccgaaaacc
      961 aaaccgcacc ccgcccggcg ggccggcggt tcaaacttta aggaacaaca atgaaaacac
     1021 tgaccgtaca cacgccttcg cacagctacc ccatctttat cggcaacggg ctgctgccgc
     1081 aggcgggaag cctgctcaaa ccgcatttgg gcaaacgcgc cgccatcatc accaacgaaa
     1141 ccgtcgcccc gctctacctc ggcacgcttc agacggcatt ggatgcggca ggcgtatccc
     1201 atttcagcat catcctgccc gacggcgagg cgcacaaaaa ctggcagacg ctcaacctca
     1261 tctttgacgg gctgatgcaa aaccgcgccg aacgtaaaac cacgctgatt gcgctgggcg
     1321 gcggcgtgat cggcgacatg gtcggctttg ccgccgccac ctaccagcgc ggcgcgccgt
     1381 tcatccaaat accgaccacg ctgttgagtc aggtcgactc atcggtgggc ggaaaaaccg
     1441 ccatcaacca cccgctcggc aaaaatatga tcggcgcgtt ttaccagccg caggcggtgc
     1501 ttgcagattt ggacacgctg cacaccctgc ccgcccgcga actgtccgcc ggtatggcgg
     1561 aagtcatcaa atacggcgcg ctcggcgaca tcggcttttt tgaatggctg gaacagcata
     1621 tgcccgaact gatggcactc gaacgcgcgc ccctcaccca agccgtgtac cgctgctgcc
     1681 aaatgaaggc agacatcgtc gcccaagacg aaaccgaaca gggcatacgc gcatggctca
     1741 acctcggaca caccttcgga cacgccgtcg aagcggaaat gggttacggc gtatggctgc
     1801 acggcgaggc cgttgccgcc ggctgcgtgt tggcggcgcg tttgtccgaa caactgggca
     1861 aaacctccgc cgcagatacc gcgcggctcg ccgccctgct cgaagccgcc ggactgccgt
     1921 ccgcaccgcc cgtgtttgcc tttgaaaaat ggctggcaca tatgagccac gacaaaaaag
     1981 tcagcggcgg catcatgcgc tttatcggtc tgaaccggct gggcgaagcc gtcatcaccg
     2041 aaattaccga cacggacatc ctccggcgca ccctgcaacc gtatctctga tttcctctgc
     2101 cgatgtgttg ccgcgcgggt ttgacgcacg atgatgactt tccatcatca ttctccgcga
     2161 aagcgggcat ccggttattc catattgcag ggatttatcc ggaataacga caacctgtcc
     2221 gccgtcattt ccgcgcagat ggaaatttgg aaattcaaaa tcacaggaat ttatccggaa
     2281 tatagcggat taacaaaaat caggacaagg cggcgggccg cagacagtac ggatagtacg
     2341 gaaccggttc gcccggtgct tgggcgcctt agggaaccgt tccctttgag ccggggcggg
     2401 gcaacgccgt accggttttt gttaatccgc aacagcaacc ctgtcgccgt cattcccgcg
     2461 aaagc
//
LOCUS       DAXFHF010000116         2456 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXFHF010000116 DAXFHF010000000
VERSION     DAXFHF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.117"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            90..467
                     /locus_tag="ACFIRM_002031"
                     /pseudo
     CDS             90..467
                     /locus_tag="ACFIRM_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            492..1028
                     /locus_tag="ACFIRM_002032"
     CDS             492..1028
                     /locus_tag="ACFIRM_002032"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3029569.1"
                     /translation="MSNTSKKSAVAGYCPNHGTWPKDNTSAGVANPTEIKGKYVQSVT
                     VANGVVTAQMKSDGVNKEIQGKKLSLWARRQNGSVKWFCGQPVKRDNANAAKDDVTAT
                     MNSSNVNKEIQGKKLSLWARRQDGSVKWFCGQPVTRNANDDTVTADATGNDGKIDTKH
                     LPSTCRDPASAVAFKSVR"
     gene            1209..1598
                     /locus_tag="ACFIRM_002033"
                     /pseudo
     CDS             1209..1598
                     /locus_tag="ACFIRM_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1732..2151
                     /locus_tag="ACFIRM_002034"
                     /pseudo
     CDS             1732..2151
                     /locus_tag="ACFIRM_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            2246..>2456
                     /locus_tag="ACFIRM_002035"
     CDS             2246..>2456
                     /locus_tag="ACFIRM_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3029570.1"
                     /translation="MPTRLDSRLRGNDEAILLAEGQKSAVTEYYLNHGTWPKDNTSAG
                     VASPPSNIKGKYVESVTVTNGVVTAK"
BASE COUNT          741 a          731 c          575 g          409 t
ORIGIN      
        1 gggaatctag gacgtaaaat ctaaagaaac cgttttcccg ataagtttcc gtgccgacag
       61 acccggattc ccgcctgcgc gggaatgacg aagccatcct tttggccgaa ggtcaaaaat
      121 cagccgtcac cgagtattac ctgaatcacg gcaaatggcc ggaagacaac ggtaatgccg
      181 gcgtggcatc cgcctccgac atcaaaggca aatatgttga aagcgttacg gtcgcaaaag
      241 gcgtcgttac cgcccaaatg aaaccaagcg gcgtaaacaa agaaatccaa ggcaaaaaac
      301 tctccctgtg gggcaggcgt caagccggtt cggtaaaatg gttctgcgga cagccggtta
      361 agcgcgacaa cgccaacgcc gacgacgtca aagccgacgg caccgacggc aaagacaaaa
      421 tcgacaccaa gcacctgccg tcaacctgcc gcgataaatc atctgccgtt tgcacgaaac
      481 accacgcgcc gatttcaaac acttccaaaa aatcagccgt tgccgggtat tgcccgaatc
      541 acggcacatg gccgaaagac aacacttctg ccggcgtggc aaaccccacc gaaatcaaag
      601 gcaaatatgt tcaaagcgtt acggtcgcaa acggcgtcgt taccgcccaa atgaaatcag
      661 acggcgtaaa caaagaaatc caaggcaaaa aactctccct gtgggccagg cgtcaaaacg
      721 gttcggtaaa atggttctgc ggacagccgg ttaagcgcga caacgccaac gccgccaaag
      781 acgacgtcac cgccacaatg aattcaagca acgtaaacaa agaaatccaa ggcaaaaaac
      841 tctccctgtg ggccaggcgt caagacggtt cggtaaaatg gttctgcgga cagccggtta
      901 cgcgcaacgc caacgacgac accgtcaccg ccgacgccac cggcaacgac ggcaaaatcg
      961 acaccaagca cctgccgtca acctgccgtg atccggcatc tgccgttgct ttcaaatcgg
     1021 ttcggtaaaa tggttctgcg gacagccggt tacgcgcaac gccaacgacg ccgtcaaaga
     1081 cgtcaccgac gccaacggca aaatcagcac caagcacctg ccgtcaacct gccgtgatac
     1141 gtcatctgcc aaataaggca aattaggcct taaatttcaa ataaatcagc catcaccggg
     1201 tattaccctc agccgtcacc gaatattacc cgaataacgg cgaatggccc gcaaacaacg
     1261 gcgctgccgg cgtggcatcc gcttcagaca tcaaaggcaa atatgttaag gaagttaaag
     1321 tcgaaaaagg cgtcgttacc gccaaaatgc tttcaagcgg cgtaaacaaa gaaatcaaag
     1381 gcaaaaaact ctccctgtgg ggcaggcgtg aaaacggttc ggtaaaatgg ttctgcggac
     1441 agccggttac gcgcaccgcc aaagacaacg acgacgtcaa agccgacggc accgccgaca
     1501 ccaaaatcga caccaagcac ctgccgtcaa cctgccgcga tgaatcatct gccgtttgca
     1561 tagaaacacc acctacggct ttctataaaa atacctaagc cgtcattccc acgaacctac
     1621 atcccgtcat tcccgcgaaa gtgggaatcc aggacgtaaa atctaaagaa accgttttcc
     1681 cgataagttt ccgtaccgac agacccggat tcccgcctgc gcgggaatga cgaagccatc
     1741 cttttggctg aaggtcaaaa atcagccgtt accgagtatt acccgaatca cggcgaatgg
     1801 ccgaaagaca acacttctgc cggcgtggca tccgcttcag aaatcaaagg caaatatgtt
     1861 gaaagcgtta cggtcgcaaa aggcgtcgtt accgcccaaa tgaaaccaag cggcgtaaac
     1921 aaagaaatcc aaggcaaaag actctccctg tgggccaggc gtgaagccgg ttcggtaaaa
     1981 tggttctgcg gacagccggt tcagcgcgcc gccaaagaca aagacgacgt caaagccgac
     2041 gacaccgccg gcaccaaaat cgacaccaag cacctgccgt caacctgccg cgacgcagca
     2101 tctgccggtt gcatagaaac accacctacg gctttctata aaaataccta aaccgtcatt
     2161 cccacgaacc tacatcccgt cattcccacg aaagtgggaa tccggaacgt aaaatctaaa
     2221 gaaaccgttt tcccgataag tttccgtgcc gacaaggctg gattcccgcc tgcgcgggaa
     2281 tgacgaagcc atccttttgg ccgaaggtca aaaatcagcc gttaccgagt attacctgaa
     2341 tcacggcaca tggccgaaag acaacacttc tgccggcgtg gcatcccccc cctccaacat
     2401 caaaggcaaa tatgttgaaa gcgttacggt cacaaacggc gtcgttaccg ccaaaa
//
LOCUS       DAXFHF010000117         2409 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXFHF010000117 DAXFHF010000000
VERSION     DAXFHF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.118"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..348
                     /locus_tag="ACFIRM_002036"
     CDS             <1..348
                     /locus_tag="ACFIRM_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LgtB"
                     /protein_id="HFC3029571.1"
                     /translation="FFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQE
                     LHYAKFHDQNSALGSLIEHDRRLNRKQQRRDSPANTFKHRLIRALTKIGREREKRRKR
                     REQTIGKIIVPFQ"
     gene            360..1280
                     /locus_tag="ACFIRM_002037"
     CDS             360..1280
                     /locus_tag="ACFIRM_002037"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC3029572.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKRMLQRWRKKLSARFLRKIY"
     gene            1340..2353
                     /locus_tag="ACFIRM_002038"
     CDS             1340..2353
                     /locus_tag="ACFIRM_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3029573.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTPAIARRFQEQDGRIRIISNPRNLGFIASLNIGLDELAKSGGGEYIARTDADDIAAP
                     GWIEKIVGEMEKDRSIIAMGAWLEVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVF
                     PFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRF
                     HQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTAYALYEKAL
                     SGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKY
                     RSY"
BASE COUNT          621 a          610 c          656 g          522 t
ORIGIN      
        1 tttttcttgg acaggtttgc cgttttgccg cccgaacgcc tgcaccctgt cgatttgatg
       61 atgttcggca accctgacga cagggaagga atgccggttt gccagctcaa tcccgccttg
      121 tgcgcccaag agctgcatta tgccaagttt cacgaccaaa acagcgcatt gggcagcctg
      181 atcgaacacg accgccgcct gaaccgcaaa cagcaaaggc gcgattcccc cgccaacaca
      241 ttcaaacacc gcctgatccg cgccttgacc aaaatcggca gggaaaggga aaaacgccgg
      301 aaaaggcgcg aacagacaat cggcaagatt attgtgcctt tccaataaaa ggagaagaga
      361 tggacatcgt atttgcggca gacgacaact atgccgccta cctttgcgtt gcggcaaaaa
      421 gcgtggaagc ggcccatccc gatacggaaa tcaggttcca cgtcctcgat gccggcatca
      481 gtgaggaaaa ccgggcggcg gttgccgcca atttgcgggg ggggggtaat atccgcttta
      541 tagacgtaaa ccccgaagat ttcgccggct tccccttaaa catcaggcac atttccatta
      601 cgacttatgc ccgcctgaaa ttgggcgaat acattgccga ttgcgacaaa gtcctgtatc
      661 tggatacgga cgtattggtc agggacggcc tgaagccctt atgggatacc gatttgggcg
      721 gtaactgggt cggcgcgtgc atcgatttgt ttgtcgaaag gcaggaagga tacaaacaaa
      781 aaatcggtat ggcggacgga gaatattatt tcaatgccgg cgtattgctg atcaacctga
      841 aaaagtggcg gcggcacgat attttcaaaa tgtcctgcga atgggtggaa caatacaagg
      901 acgtgatgca atatcaggat caggacattt tgaacgggct gtttaaaggc ggggtgtgtt
      961 atgcgaacag ccgtttcaac tttatgccga ccaattatgc ctttatggcg aacgggtttg
     1021 cgtcccgcca taccgacccg ctttacctcg accgtaccaa tacggcgatg cccgtcgccg
     1081 tcagccatta ttgcggctcg gcaaagccgt ggcacaggga ctgcaccgtt tggggtgcgg
     1141 aacgtttcac agagttggcc ggcagcctga cgaccgttcc cgaagaatgg cgcggcaaac
     1201 ttgccgtccc gccgacaaag cgtatgcttc aaagatggcg caaaaagctg tctgccagat
     1261 tcttacgcaa gatttattga cggggcaggc cgtctgaagc gttcagacgg catcggacgt
     1321 atcggaaagg agaaacggat tgcagccttt agtcagcgta ttgatttgcg cctacaacgt
     1381 agaaaaatat tttgcccaat cattggccgc cgtcgtgaat cagacttggc gcaacttgga
     1441 tattttgatt gtcgatgacg gctcgacgga cggcacgccc gccattgccc ggcgtttcca
     1501 agaacaggac ggcaggatca ggataatttc caatccccgc aatttgggct ttatcgcctc
     1561 tttaaacatc gggctggacg aattggcaaa gtcggggggg ggggaatata ttgcgcgcac
     1621 cgatgccgac gatattgccg cccccggctg gattgagaaa atcgtgggcg agatggaaaa
     1681 agaccgcagc atcattgcga tgggcgcgtg gttggaagtt ttgtcggaag aaaacaataa
     1741 aagcgtactt gccgccattg cccgaaacgg cgcaatttgg gacaaaccga cccggcatga
     1801 agacattgtc gccgttttcc ctttcggcaa ccccatacac aacaacacga tgattatgag
     1861 gcgcagcgtc attgacggcg gtttgcggtt cgatccagcc tatatccacg ccgaagacta
     1921 taagttttgg tacgaagccg gcaaactggg caggctggct tattatcccg aagccttggt
     1981 caaataccgc ttccatcaag accagacttc ttccaaatac aacctgcaac agcgcaggac
     2041 ggcgtggaaa atcaaagaag aaatcagggc ggggtattgg aaggcggcag gcatagccgt
     2101 cggggcggac tgcctgaatt acgggctttt gaaatcaacg gcatatgcgt tgtacgaaaa
     2161 agccttgtcc ggacaggata tcggatgcct ccgcctgttc ctgtacgaat atttcttgtc
     2221 gttggaaaag tattctttga ccgatttgct ggatttcttg acagaccgcg tgatgaggaa
     2281 gctgtttgcc gcaccgcaat ataggaaaat cctgaaaaaa atgttacgcc cttggaaata
     2341 ccgcagctat tgaaaccgaa caggataaat catgcaaaac cacgttatca gcttagcttc
     2401 cgccgcaga
//
LOCUS       DAXFHF010000118         2302 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXFHF010000118 DAXFHF010000000
VERSION     DAXFHF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.119"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(93..1727)
                     /gene="groL"
                     /locus_tag="ACFIRM_002039"
     CDS             complement(93..1727)
                     /gene="groL"
                     /locus_tag="ACFIRM_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC3029574.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(1820..2110)
                     /locus_tag="ACFIRM_002040"
     CDS             complement(1820..2110)
                     /locus_tag="ACFIRM_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC3029575.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          426 a          629 c          619 g          628 t
ORIGIN      
        1 ttaaggtttg cgccttagcc gcacttattc gcaaaaaaac cgcacggcgt tgaccgtgcg
       61 gcttgttgtc tgaaggtttc agacggcatt gcttacatca tgccgcccat accgcccatt
      121 ccccccatat cgggcacagc cggtttttct tcagggattt cggcaatcat gcagtcggtc
      181 gtcagcatca gaccggcgat agacgcggcg tgttgcagcg cggaacgggt tactttggca
      241 gggtcgagta cgcccattcc gatcatgtcg ccgtattcgc cggagcctgc gttgtaaccg
      301 tagttgcctt tgccttccaa cactttgttc accaccacgc tgggttcgcc gcctgcgttg
      361 gcaacgattt ggcgcagcgg agactcaacg gcgcgcaata cgatttgtac gcctgcgtct
      421 tggtcggcat tgccggtgtg caggttttcc aaagcggcac gggcgcgcaa cagggctacg
      481 ccgccgcctg caaccacgcc ttcttcaacg gctgcgcggg tagcgtgcag cgcgtcttcc
      541 acgcggtctt ttttctcttt catttcgact tcggtcgccg cgccgacttt gatcactgcc
      601 acgcctcctg ccagtttggc aacgcgctct tgcagttttt ctttgtcgta atcgctggtc
      661 gcggtttcga tttgttggcg gatttcggca acacgcgctt cgatttgggc tgcgtcgccg
      721 aagccgtcga tgacggtagt gttttcttta ccgatttcga tgcgtttggc ttgacccaag
      781 tcgtccaaag tcgctttttc caaagacagg ccgacttctt cggaaatcac tacgccgccg
      841 gtcaggatgg cgatgtcttg cagcatcgct ttgcggcggt cgccgaagcc gggggctttg
      901 acggcaacgg ttttcaggat gccgcggatg ttgttcacga ccaaagtcgc caaggcttcg
      961 ccttctacgt cttcagcgat aatcaacagc gggcggctgg ctttcgccac ttgttccaac
     1021 acgggcagca ggtcgcggat gttgctgatt tttttgtcga acagcaaaac aaacggattg
     1081 tccagaccgg cgatttgttt ttccgcgtcg ttgatgaagt aaggggacag gtagccgcgg
     1141 tcgaactgca taccttcaac cacgtccagc tcgttttcca aagatttgcc gtcttcaacg
     1201 gtaatcacgc cttctttgcc gactttttcc atcgcttcgg cgataatcgc gccgacttgt
     1261 tcgtcggagt tggcggaaat cgagccgact tgggcgattt ctttggaagt atcgcaaggt
     1321 ttggcgatgt ttttcagctc gtcaaccaaa gcggcaacgg ctttgtcgat gccgcgtttc
     1381 agatcggtcg ggttcatgcc ggcggtaacg tatttcatgc cttcggcaac gatggattgt
     1441 gccaatacgg tggcggtagt cgtaccgtcg ccggctacgt cgttggtttt ggacgcgact
     1501 tctttcacca tttgcgcgcc catattttca aacttgtctt tcagttcgat ttctttggcg
     1561 acggttacgc cgtctttggt gatgtgcggg ccgccgaaag cgcggtcaac caccacgttg
     1621 cggcctttgg ggcccaaggt tacgcgcacg gcgtttgcca aaatattcac gccgtttacc
     1681 attttttggc ggacttcatt gccgaattgt acgtcttttg ctgccatttc aattctccaa
     1741 aaatcattaa aactgtctga taaaaccgtt tatgccgtct gaaggcggtt tgccgtttca
     1801 gacggcatcg tgtccgtatt tatttttcaa cgatgccgaa aatatcttct tcgcgcatta
     1861 ccaacagctc ttcgccgtcg gcttttacgg tttggccgct gtatttgccg aagatgattt
     1921 tgtcgccgac tttgacatcc agcggacggc gcgcgccgtc tttgccgatt ttgcccgcgc
     1981 ccacggcgat gacttcgccc atatcgggtt tttcggcggc cgcacccggc aggacgatgc
     2041 ccgatgcggt tttttcttca gcttccaagc gtttgacgac gacgcggtcg tgtaaaggac
     2101 ggatggtcat atttatgctc cgataaatgg tttgaaaaca atcatctgcc cgaacggttc
     2161 ggacaaattg aagtggaaac cgggctgccg cctaacggcc tgcccgtaca agtcggcaaa
     2221 attagggtgt gtgcgggaaa attcaagtga ggcggaaaaa aatttatttc cgccgttttt
     2281 tatagtggac taaatttaaa cc
//
LOCUS       DAXFHF010000119         2265 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXFHF010000119 DAXFHF010000000
VERSION     DAXFHF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.120"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            112..336
                     /locus_tag="ACFIRM_002041"
     CDS             112..336
                     /locus_tag="ACFIRM_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029576.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            365..1651
                     /locus_tag="ACFIRM_002042"
     CDS             365..1651
                     /locus_tag="ACFIRM_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC3029577.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQLGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDREWQHIKDIEYEERQISDFLNN"
     gene            1665..1976
                     /locus_tag="ACFIRM_002043"
     CDS             1665..1976
                     /locus_tag="ACFIRM_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029578.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            1990..2193
                     /locus_tag="ACFIRM_002044"
     CDS             1990..2193
                     /locus_tag="ACFIRM_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029579.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          735 a          445 c          535 g          550 t
ORIGIN      
        1 ggagcggata aattctcgca atctaaaacc ttatatattg cgctccataa ggggcgcata
       61 tttttaatcc ttgtaaagcc ccttaactct agtcagtgaa ggggcttaat catgtttatt
      121 ttctatactg tcaatcccga acacgtatat ttccccaaag catacatcat gaaagtgttc
      181 aaggataaag gctacgaatc ccaatgcatc acaacagttt ctttttatat ctgcaatccg
      241 actttgaagc aaaagaccga aaacgaagcg tatgaatacg gcagattgtt tgttaaagaa
      301 ctgatgcata aggaatgcaa ccgtgaaagc ctttgaagat aaagccttgg cggtgtccgc
      361 ggacatggcg gacgcgttag cggacggcgg ggctgcggca accgccgtag gctgcccccc
      421 taggctaata gggggggagc aaaataaaac cccaaatccg aagggtgcgg aaaaaacaga
      481 aaaccaagaa tttcaattcg agtatttcag tcattttgta tcggacggca aaggcagatt
      541 tatagaaata ccgttaaggc gtggcaggga tgacggagcg ttcatagacc aaatcacttt
      601 cacgatacat gaaaattcca tgacgaaagt aaccggaaag ggattggtat cagatacgga
      661 attcgttgtc cggtatagcg agctgcttga agaaatcttg ggattcggca ttaccaaaaa
      721 attaccgttt aaaggaaagt ttttctatca aagctgttat caattcgggc ccgataacgt
      781 cgaatacgga aaggttcatt acggcggcca atgtgaaacg atgttggttg aattgaacgg
      841 tacaggctgc atggctgctt tacccggttg ggaaaaccga ttgtatgaat ttttaagtaa
      901 atgcgtccga cccaaaataa cccgtatcga tgtcgcccat gattttttca acggagagta
      961 cacacccgat caggcaatgt tagaccacga taacggacat tacgacgtac acaatatgcg
     1021 gccaaaaagc gaatgtcgcg gcacggcatg gcgcaatgaa gacggcagtg gaaaaacctt
     1081 ttacatcggc aagcgcggaa attcaaaatt tacccgtgtt tacgagaaag gcagacagct
     1141 tggagatgtt gatagccctt gggtcaggtt tgagggggat atagaaatac ccttggatgt
     1201 cctactttat tcggggtcgt atctaggcgg cgcatatccg atttgcaagg agatttttaa
     1261 gacagaagcc aagcgcatgg aagtaaaagt taaaaacgtt aatctgatat tcgacgttaa
     1321 gctttttcat gcaaggaatc aagtcggcaa gatggttaat ttcctccgcg atatagggtg
     1381 ggacgatagc aggatagttg atgagctggt gaaaggcgtt gaaggttatc caaaaggttt
     1441 acagcctgag caatacgatt gcaaaaacca aacgcagaaa ttccagtata tccacgaaga
     1501 acagaaagcc attaacgcat tgaacattga aacactgttt gatgatttga ttgaagagag
     1561 agaatacgca tttcctcaag acagggaatg gcaacacatc aaggatattg agtacgagga
     1621 acgtcaaata tccgattttt taaacaactg aaggaaattg aaaaatgttt gaaacaagcc
     1681 aagtaaccac atataccgcc actttgttgg gcgcaaaaaa attcaaaggc gaaatcgacg
     1741 gtaacaaaat cgattcatgc accgtcttgg tagccagccc catgccgtca aacggcaatg
     1801 ccgtgggctt tacctcggaa agcatgaagt ttggtgacag ccataatttt gaaaaattga
     1861 aaaaccttaa attcccctgt gcggttgatc taaccgttgc catggaatca acaggaaaag
     1921 gcttagtgca gaaattgttg gatttccaag taaagggcgc agcacctaaa gcctaagaaa
     1981 ggctgaatca tgagcagata ccaacagaaa tttatcgtac aggaattgga aaatcacgaa
     2041 ttcatctatc ccgattcgtt tggcgatatt ggatttacgt cgaacatcaa atcagccggt
     2101 aaatatgaca gctatgaaga cgcttttagc tcggcattgg aagagatagg cggggaattt
     2161 gtaattttcg gattctacga aaaagaagat taagtttaag aggctcggca ggcggtctct
     2221 aaaaccttca caaagcccgc aaacacattt ttttaaaaat ttcgt
//
LOCUS       DAXFHF010000120         2016 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXFHF010000120 DAXFHF010000000
VERSION     DAXFHF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.121"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..866)
                     /gene="ispH"
                     /locus_tag="ACFIRM_002045"
                     /gene_synonym="lytB"
     CDS             complement(<1..866)
                     /gene="ispH"
                     /locus_tag="ACFIRM_002045"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC3029580.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGH"
     gene            complement(896..1390)
                     /gene="lspA"
                     /locus_tag="ACFIRM_002046"
     CDS             complement(896..1390)
                     /gene="lspA"
                     /locus_tag="ACFIRM_002046"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC3029581.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          460 a          577 c          515 g          464 t
ORIGIN      
        1 tcatgccccc atccgcgtat ggctgccaat acttcccgca ccaacacttc gggcgcggac
       61 gcgcctgccg ttacgccgac tttgcttttg ccttcaaacc acgtgcgttg caggtaggac
      121 gcgttgtcca ccatatacgc atcgattccg cgcgatgccg ccacttcgcg caggcggttg
      181 ctgttggacg aattgggcga accgaccaca atcacgatgt cgcactgttc cgccagctct
      241 ttgacggcgg tttgccggtt ggtcgtcgca tagcagatgt cttccttgtg cggattgcgg
      301 atattgggga aacgcgcgtt cagcgcggcg atgatgtctt tggtttcatc gaccgagagc
      361 gtggtttggc tgacataggc gagtttgtcg gggtttctga cttcgagttt tgccacatct
      421 ccgaccgttt cgaccaaaag cattttgccc ggtgcaagct gccccatcgt gccttcgacc
      481 tcggcgtgcc ccttatgccc gatcatgatg atttcacagt tttgggcatc cagtcgggcg
      541 acttccttat gcactttcgt caccagcggg caagtcgcat caaatacccg gaaaccgcgc
      601 tccgccgctt cctgctgcac cgccttcgat acgccgtgtg ccgaataaac cagtgtcgcg
      661 cccggcggca cttccgccaa gtcttcgata aacaccgcgc ctttttcgcg caggttgtcc
      721 acgacgaatt tgttgtggac gacttcgtgg cgcacataaa ccggcgcgcc gaattcttcc
      781 aaagcacgtt cgacaatact gattgcccga tccacaccgg cgcagaagcc gcgcggattg
      841 gcaaggatga tggtttttcc gttcataagt tttgcattcc gtgttcagac ggcattcacg
      901 tttttttgct atctttgcga tggacgatat tgtcaagcac cgccaacacc gcaccgacgc
      961 agataaagct gtcggcaata ttaaaggcgg gataaaacca attttgccaa taaaacaata
     1021 agaaatcgac gacatgaccg tgtatcaggc gatcgatgac attgcccgac gcaccgccga
     1081 taatcattgc cgccccgatt ttgccgaggg ctgcaaactc gtcgcgcaag atggcgcgta
     1141 ccaaatacgc gctcaccgcc accgccagca ccaaaaaaaa gtatttctgc cagccgccct
     1201 gatcggcaag gaagctgaat gccgcgcccg ggttgtacac cagcgtcaga tcgaaaaacg
     1261 aagggatgac gttgacgcgt tcccgatact gaaacgacga cagcaccgcc cacttcgacc
     1321 actggtccag cacgatggcg gcaagtgcca atacccaata gcgcgtttta cttgaaacag
     1381 atgaagacat atttttcaac agccggataa aagagtgcca ttctacccga aaaccccctt
     1441 tcctgtaccc gaaacggcaa atgccgtaat cttgaaaccc gtcattcccg cgcaggcggg
     1501 aatccggacc tgtccgcacg gaaacttatc ggataaaacg gttgcccaaa ccccgcgtcc
     1561 tagattccca ctttcgcggg aatgacggtt cggttgcgta gggccgggtg gtcgaaaagg
     1621 cggattcgat ggattcgatg aaaacggtag acatgttgga ttgatggaaa tggcgggccg
     1681 aagcacaccc tacggcccgc cggatttccg cctgcgcggg aatgacgggg tttcaggttg
     1741 ctgtttcagg ttgctgtttc aggttgctgt ttcaggtttc tgtttttaag tttctgtttc
     1801 ggtttgatat tttatagaaa tgacgaaatt ttagattgca agaacttatc ccctccgccg
     1861 tcattcccac ggaagtggga atctagaaat aaaaagcagc aggaatttat cggaaataac
     1921 tgaaaccgaa cggaccggat tcccgcctgc gcgggaatga cgaatccatc cgcacggaaa
     1981 cctgcaccac gtcattccta cgaacctaca tcccgt
//
LOCUS       DAXFHF010000121         1978 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXFHF010000121 DAXFHF010000000
VERSION     DAXFHF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.122"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(166..588)
                     /locus_tag="ACFIRM_002047"
     CDS             complement(166..588)
                     /locus_tag="ACFIRM_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3029582.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(594..1190)
                     /locus_tag="ACFIRM_002048"
     CDS             complement(594..1190)
                     /locus_tag="ACFIRM_002048"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC3029583.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            complement(1380..>1978)
                     /locus_tag="ACFIRM_002049"
     CDS             complement(1380..>1978)
                     /locus_tag="ACFIRM_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3029584.1"
                     /translation="ASPCGRIPTGKKGLGKLALFRLGNKIEISTIQGNERVTFTLDYA
                     EIKKSERIYQPEFQKESVKPNTENGTTITLTELTKKQGYPLDNYVGHLSRLFDFPAQD
                     FKIKVSLNGSEPRIIDGNLKYNLVTPQFEWEYQDLATNISSLSSKFEQYEYSGLIQGK
                     FITTEKPLKNNMKGITLFANGRMVNMPEFFTDSESSHF"
BASE COUNT          537 a          413 c          325 g          703 t
ORIGIN      
        1 cctttgtcat cccttgctta tctcccattt gcgaatcctc aaaaacgggc aaaaaaaagc
       61 cctgttactt gtagaaagta aagggcgtta atttttgtta atcacccctt cttagggacg
      121 caatatataa gggtcttaat taaaggaaga tgccgtctga ttttttcaga cggcatcttt
      181 tatttcctcg accaaccgat ttaatcgctc cctacaaata gctttatttt ttaattcgca
      241 ctcccaaacg acaatcactt tgaaaccaat cttttccagc tcggtttcat ttttaatatc
      301 acgttcgcga ttttttgtga ttttttccaa ccaaaaatcc atattgcttt tcgggatatg
      361 tcctttatta caagaatgcc cgtgccaaaa acagccgtgt ataaacacga ctgttttata
      421 tttaggcaaa acgatatccg gtttgcctgc atagcgctta tcgtttttcc gataacggaa
      481 accttgggaa aaaagaaatt tccttaccaa tacttccggt ttggtttctt tgctatggat
      541 ttttgacatc acgaaggaac gtttggatgg agtgaaaata tcggtcataa aatttaaggc
      601 gatgtaggtt tagctcgttt ttttgtttgt tccacttttc tcaataaata tgaaactaag
      661 ctgattaaat ccaaagcatc cttgtcatta aaaatactag gagataaatt tgctcttaac
      721 tcatgttgca caggatttct aaatccagtc catgttccgc atgcaagctg ttcaagacca
      781 tcttctaaat tttgttccgc ctgcgttttg ttattcgtta atagaagcat agatttttta
      841 ctcccgaagg ctttttcaat caattctctt ccgtctatgc tatgaagacc tgtctgcttc
      901 tgaaccttat cttcgaaaac tttaactgcc tcgattgcag cacttaaata atccttcttt
      961 tcattgtaaa aatcgttaca ggcagtatgc aaatcctgat gcaaatgccg ccaatgatag
     1021 taaggatatt ctggaataat actatgtaag gcagagatta cttccgcttg ttctttattt
     1081 gtaagctccg aatcttccaa acgattcaaa ataacttcta ccggttctct ttccttttct
     1141 ggcaatttgt ttttccaatt ttcgatatca actccgcttt cacctttcac ttcgtaatct
     1201 ttctcttttt ttcttctttt tctccaatct tgcccgatgg ctcagacgat ttccccaagg
     1261 aaaacccgta attttgaagt tttaggatgc ttccaatcca atgatgtcct atctgtagcg
     1321 attaaatctt caccatcatt atctataaaa tcaatactta accatcctgt cagataggat
     1381 tagaaatggc tggattcgct atcagtgaaa aactcgggca tatttaccat tctgccgttg
     1441 gcaaacaagg taataccttt catattattc tttaaaggtt tttccgttgt aatgaactta
     1501 ccttgtatta atccgctgta ttcatactgt tcgaattttg aagataacga tgaaatattg
     1561 gttgctagat cctggtattc ccattcgaat tgtggggtaa caagattata ttttaggttt
     1621 ccgtcaatga ttcttggttc cgagccgttc aagcttactt tgattttaaa atcctgagcc
     1681 ggaaaatcaa ataaacggga aagatgcccc acataattat ctaacgggta tccttgtttt
     1741 ttcgtcagct cggttaaagt tatagttgtt ccgttttcgg tattgggttt aacagactct
     1801 ttctgaaact ccggttgata aatacgctca ctttttttaa tctctgcata atccaaagta
     1861 aaagtaaccc gttcgtttcc ttggatagta gagatttcga ttttgttgcc aagcctgaat
     1921 aatgccaatt taccaagacc ttttttaccc gttggaattc ttccgcacgg ggaggctt
//
LOCUS       DAXFHF010000122         1964 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXFHF010000122 DAXFHF010000000
VERSION     DAXFHF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.123"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            38..391
                     /locus_tag="ACFIRM_002050"
     CDS             38..391
                     /locus_tag="ACFIRM_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029585.1"
                     /translation="MPSEPSDGIFDALAVYGRGAGAGKYPYRHCRYTVTLKPAIPAQA
                     GIRICRDSLKSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMELKHCKEMLEQ
                     KDKEIELLRKLTETV"
     gene            480..1049
                     /gene="rsmD"
                     /locus_tag="ACFIRM_002051"
     CDS             480..1049
                     /gene="rsmD"
                     /locus_tag="ACFIRM_002051"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC3029586.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1172..1423
                     /locus_tag="ACFIRM_002052"
     CDS             1172..1423
                     /locus_tag="ACFIRM_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC3029587.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1520..1603
                     /locus_tag="ACFIRM_002053"
     tRNA            1520..1603
                     /locus_tag="ACFIRM_002053"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Tyr,seq:gta)
     gene            1634..1707
                     /locus_tag="ACFIRM_002054"
     tRNA            1634..1707
                     /locus_tag="ACFIRM_002054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1666..1668,aa:Gly,seq:tcc)
     gene            1717..1791
                     /locus_tag="ACFIRM_002055"
     tRNA            1717..1791
                     /locus_tag="ACFIRM_002055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1749..1751,aa:Thr,seq:ggt)
     gene            1840..>1964
                     /locus_tag="ACFIRM_002056"
     CDS             1840..>1964
                     /locus_tag="ACFIRM_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3029588.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGG"
BASE COUNT          540 a          455 c          508 g          461 t
ORIGIN      
        1 ttgggcatgc gctaccgctt ctgatccccc gattccaatg ccgtctgaac cttcagacgg
       61 catttttgac gcgctcgccg tttacgggcg cggggcaggt gcggggaaat acccgtaccg
      121 ccattgccga tacaccgtaa ccctaaaacc cgccattccc gcgcaggcgg gaatccggat
      181 ttgtcgggat tcgctcaaat cgggtggcgg agggatggtg ttgcagatta acgatgtgga
      241 taccaacagc ggggaatttg caatctatac cgctcaggat gcatcggtaa agctggattt
      301 gttaaaaatg gaattgaaac actgcaaaga aatgttggaa caaaaagaca aagaaatcga
      361 gttgctccgc aagctgaccg aaaccgtcta aacagatatg ccgtctgaaa aaagttttca
      421 ggcggcatat tcttcgacag gtcttgtata ataccgtttg aactttcagg cttttgatta
      481 tggcggcagg caaacatacc aaacacagca accgggtacg cattatcggc gggcaatgcc
      541 ggggcaggaa attgagtttt gcatccgccg acggactgcg cccaaccccc gacagcgtgc
      601 gtgaaaagct gtttaactgg ctgggacaag atttgacggg aaaaacggtt ttggatctct
      661 tcggaggcag cggcgcactc ggcatggagg cggcatcgcg taatgcaaaa cgggtcgtga
      721 ttgcagacaa caaccgtcaa acggtgcaaa cccttgagaa aaacagccgc gaactgggtt
      781 tggggcaggt gcaaaccgtc tgttcggacg gcattgccta tttggcaaac ctaaaagaga
      841 aattcgatgt cgtcttcctt gatccgccgt ttgcgtggca aagttgggaa agtcttttca
      901 atgtattggg cacacgcctg aatgacggcg catatgttta tatcgaggca ggcaggcagc
      961 cggacaaacc tgattggctg acgggatata gggaggggaa gtcggggcag ggtacatttg
     1021 aattaagggt tttccaagtg gctgaataat atgcgctttg ataatcattt ccgggttgta
     1081 aacattcgtt tgcaaccgtc cggttcaaaa aaaccttgtg ctataatccg cgcccgcccg
     1141 gttttgataa tttagtggaa aaggaaaaga aatgtcgctc tttattaccg acgagtgcat
     1201 caactgcgac gtatgcgaac ccgaatgccc caatgatgcc atttcccaag gcgaggaaat
     1261 ttacgaaatc aaccccaacc tctgcacgca gtgcgtcgga cactacgatg agccgcagtg
     1321 ccagcaggtt tgcccggtgg actgcatcct gattgacgaa gaacatcccg aaacccatga
     1381 cgagttgatg gcgaaatacg aaaagattat ccagtttaaa taaattcttt ttaaaacatc
     1441 aaattatgtc tgttttgaaa taaaatcaaa aaaaaacttg acggaaaagc aagccgctaa
     1501 taaactagtg ttctcttttg gagggattcc cgagcggtca aagggggcag actgtaaatc
     1561 tgttgcgaaa gcttcgaagg ttcgaatcct tctccctcca ccaaaattct tacttggggc
     1621 agtagcgagt aatgcgggtg tagctcaatg gtagagcaga agccttccaa gcttacggtg
     1681 agggttcgat tcccttcacc cgctccaaac aattaggccc atgtagctca ggggtagagc
     1741 actcccttgg taagggagag gtcggcagtt caaatctgcc catgggcacc atctctcgat
     1801 tattcatttc tttaaggctt agatatatag gatattgcca tggctaagga aaaattcgaa
     1861 cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg taaaaccacc
     1921 ctgactgctg ctttgactac tattttagct aaaaaattcg gcgg
//
LOCUS       DAXFHF010000123         1937 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXFHF010000123 DAXFHF010000000
VERSION     DAXFHF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.124"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(80..439)
                     /locus_tag="ACFIRM_002057"
     CDS             complement(80..439)
                     /locus_tag="ACFIRM_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029589.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RVARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(560..1645)
                     /locus_tag="ACFIRM_002058"
     CDS             complement(560..1645)
                     /locus_tag="ACFIRM_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC3029590.1"
                     /translation="MIYLFTGNMGAGKTPRVVSMILNNEDGLFKMKLEDGTEADRPLY
                     FCHIDGLDKRKFNARELAEGQIMSAPLRDVIPEGAVLIVGEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTRHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEAANWKPPKEAFKYYKSASRHQKFKKKVPWAVWALIAVVGFVGWKSYGMF
                     QVYSKATDSRIEQEAQKESVVQTMTEQTASSETAPFEHSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DERQRTEQAAQSAKADKPQVLVMGGKS"
     gene            complement(1655..>1937)
                     /locus_tag="ACFIRM_002059"
     CDS             complement(1655..>1937)
                     /locus_tag="ACFIRM_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3029591.1"
                     /translation="LSGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFKGYT
                     ANAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
BASE COUNT          459 a          473 c          432 g          573 t
ORIGIN      
        1 acccccttca ctccgcccaa gccatcctga ggggtagtgg ctgaatttgt gattttggtt
       61 ttatcagatt ttggttttat caaacaaaat atttgactga agtcacatgg cggtcgtcat
      121 atgggggttc cttcgcaccc aaaaaatcga ccgcgtaacg gtttactaaa acttcatcgt
      181 ccttaaactt ttggtgcttt ttccggcaat attttctgaa ctccgttaaa tttgatggca
      241 agaacccgca accgtctgca ccgtaaatgt aatcaacttc aaacaaactg tctttccggg
      301 caacccgacc atctttaagc cagaacatag gctcgaaata aaaaactttc gacgtgtccg
      361 ggaacttatg gagacggaga acacgtgtga aatccccgtt ttcatctaca attttgatat
      421 atcttggttt gtgaatcatg acatcctcag atttagtatt cagaatatga ttttaaaaag
      481 aactttctgc tttacgactc cgccgccgat tccttcaaac ggttttccgc gctcttcagt
      541 tgtcgtacat taaattttat taggactttc cgcccattac gagaacttgg ggcttgtccg
      601 ctttcgcgga ctgtgccgcc tgttccgtcc tttgccgttc gtccttgtaa ggattgaaag
      661 gcaacccgtt tttcacatat tctttacaca ttatctttgt tatttctttc aagggtgttc
      721 cttgatttga atagcatgtg caatctgatt ttccgccgtc tatgcatccg gcgatttgct
      781 caaaggtttt tacttgtcgg actgtgttat aaataggctt gctttcgggc ttttcgggca
      841 aagtcggcac aaagtcttca ggtttcagat tgtcggaatg ctcaaaaggc gctgtttctg
      901 atgatgccgt ctgctccgtc atcgtctgca caacgctttc tttttgcgct tcctgctcaa
      961 tccggctgtc tgtggctttg ctgtaaactt gaaacatgcc gtaacttttc cagcctacaa
     1021 accctacaac cgcaatcaac gcccaaaccg cccaaggcac ttttttcttg aacttttggt
     1081 gccggcttgc ggatttatag tatttgaagg cttctttagg cggtttccaa tttgcggctt
     1141 ctacgccgct cacgcctgcc ggattgtcca acgaggttac gcatttatac caataatact
     1201 gtttcatgcc gattgccttg cgttcaaggt gtacatgctt tgaaacaagg ttgcggacga
     1261 atatatcaag ttggctcggg tgccgcgtca tcaaaatgac ggtatgcccg tgatggcgga
     1321 gttctgtcag ttcctgaata tagggcggaa cgggacggcc tgccgcgcgt accgggtaag
     1381 tgtagtgcgc ttcgccaaca atcagcaccg cgccttccgg tatgacatca cgaagcgggg
     1441 cggacatgat ttgcccttcc gccagttcgc gggcattgaa ttttcgtttg tccaatccgt
     1501 cgatatggca gaaataaagc ggccggtctg cctccgtgcc gtcttccaat ttcattttga
     1561 acaatccgtc ttcgttgttc aaaatcatag agacgacacg gggggttttg cctgccccca
     1621 tgtttcccgt aaacagataa atcatgcttc tacctcatcc cggaaagaca aacgtcagtt
     1681 ttttgaatgc gtgcatacca atgaagaacg agaatgcgcc gaacaggtag cccaacccct
     1741 gaccgaatcc cgaaattaaa agaaggttca atatgtcgga aggcatggaa ttgatcgcat
     1801 ttgccgtgta gcctttgaac ttttccagtg cggcgagata cccggcatag gttacgaatg
     1861 tcagacctgt tgcaaggatg attctgacaa tcagcatttt cagaagtatg cctaaaagtg
     1921 gaatcaggcc ggaaagt
//
LOCUS       DAXFHF010000124         1928 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXFHF010000124 DAXFHF010000000
VERSION     DAXFHF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.125"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            883..1236
                     /locus_tag="ACFIRM_002060"
                     /pseudo
     CDS             883..1236
                     /locus_tag="ACFIRM_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1254..1580
                     /locus_tag="ACFIRM_002061"
                     /pseudo
     CDS             1254..1580
                     /locus_tag="ACFIRM_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1621..1928
                     /locus_tag="ACFIRM_002062"
                     /pseudo
     CDS             1621..1928
                     /locus_tag="ACFIRM_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          550 a          530 c          472 g          376 t
ORIGIN      
        1 aaataatcac gcatccgggc attcaatatc gtcggcagtt tgcgcataca tgccgtaacg
       61 gcaaccttat acggcttacc cttgtatggc ggattaacaa aaatcgggac aaggcggcgg
      121 gccgcagacg gtacaaatgg tacggaaccg atccgcccgg tgcttcatca ccttagggaa
      181 tcgttccctt tgagctaagg cggggcaacc cgtaccggtt tttgttaatc cgctatacag
      241 caggcgttgg tggaaatccc gaataagcgg ttcaaaacgt gtcgctgccg cggcagccat
      301 atacagttgg caactgtccg gttgtgccga tgggttgccc tgccgctccc tgagctacgc
      361 aacggttgtc tgccttgggc gggcgcgggt ggtggccggg cggtttgtcg ctatgatacg
      421 gaagttccga tatacaagcg gtgggctgaa gcctaccatt tccatcaatc caacatttct
      481 accgttttca ttgaatccat cgaatccgcc cctttcgaca atccgaccct atacaactga
      541 accgtcattc ccgcacaggc gggaatctag acccgtcggc acggaaacct gcgtcccgtc
      601 attcccacaa aagtgggaat ccggaacgca aaatctaaag aaaccgtttt acccgataag
      661 tttccgcact gacagacctg gattcccgcc tacgcgggaa tgacggtata tcggtttccg
      721 ttcggacaaa tgtctgcgca ggaataaatg gccgcgtttg aattctcggt taattccatt
      781 tattagccgc ttgcttctgt ttgcatgaaa gccgggatta ttgaataaaa tacatttgca
      841 cggccggtta ggtttgcccg ccggaatatt aaaacaccgc caaaaaaatc agccgttacc
      901 gaatattacc tgaatcacgg catatggccg aaagacaaca cttctgccgg cgtggcatcc
      961 gcttcagaca tcaaaggcaa atatgttaag gaagttaaag tcgaaaaagg cgtcgttacc
     1021 gccacaatgg cttcaagcaa cgtaaacaaa gaaatccaag gcaaaaaact ctccctgtgg
     1081 gccaagcgtc aagccggttc ggtaaaatgg ttctgcggac agccggttaa gcgcgacaaa
     1141 gccgacgccg acaacgacgc cgtcaccgac gacgccaacg gcaacaacgg catcaacacc
     1201 aagcacctgc cgtcaacctg ccgcgatgaa tcatcgttgc cgggtattgc ccgaatcacg
     1261 gcatatggcc gaaagacaac acttctgccg gcgtggcatc ttcttcatca atcaaaggca
     1321 aatatgttaa agaggttgaa gttaaaaacg gcgtcgttac cgccacaatg ctttcaagcg
     1381 gcgtaaacaa agaaatcaaa ggcaaaaaac tctccctgtg gggcaggcgt gaaaacggtt
     1441 cggtaaaatg gttctgcgga cagccggtta cgcgcaccgc cggcgccaac aacaacgacg
     1501 aagtcgccaa agccggcgcc gacaacgaaa aaatcaacac caagcacctg ccgtcaacct
     1561 gccgcgatga atcaactgcc gtttgcatag aaacaccacg cgccgatttc aaacacttcc
     1621 aaaaaatcag ccgttaccga gtattacctg aataacggcg aatggccgaa agacaacgac
     1681 aaggccggcg tggcatccgc ttcagacatc aaaggcaaat atgttaagga agttgaagtc
     1741 aacaacggcg tcgttaccgc cgaaatgaaa tcagacggcg taaacaaaga aatcaaaggc
     1801 aaaaaactct ccctgtgggc caagcgtgaa aacggttcgg taaaatggtt ctgcggacag
     1861 ccggttaagc gcgacaacgc caacgccgcc gtcgacgccg acaccgccga cgacaccaaa
     1921 aaaatcaa
//
LOCUS       DAXFHF010000125         1794 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXFHF010000125 DAXFHF010000000
VERSION     DAXFHF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.126"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..1197
                     /locus_tag="ACFIRM_002063"
     CDS             <1..1197
                     /locus_tag="ACFIRM_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC3029592.1"
                     /translation="EVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQITFSIHEDTF
                     FHVYGLSFDLFDDDDFIRAASAKMEEIFGFGIIEKAKHSGGRFYEGCWLMGTENAQYG
                     RVHYGGQRETMLVELTAVGCNAANIGWESRLFDFLTNAIRPKITRVDIAKDFFNGEYS
                     PNQAREDRNKGLFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYE
                     KGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFAQKATRIHAV
                     KEDKVVSADRCLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLALEAY
                     DCAFLKSQVIHEQPAFKPYNDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFAR
                     LPISWA"
     gene            1242..1532
                     /locus_tag="ACFIRM_002064"
     CDS             1242..1532
                     /locus_tag="ACFIRM_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029593.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            1537..1734
                     /locus_tag="ACFIRM_002065"
     CDS             1537..1734
                     /locus_tag="ACFIRM_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029594.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
BASE COUNT          542 a          403 c          410 g          439 t
ORIGIN      
        1 gaagtcttcg aatgttacga aacgtacata acggacggta aaggaaacct gttaggcgtt
       61 cctcttaggc gcggtgtatc agattcggct ttcattgacc aaatcacatt ttcaattcat
      121 gaagacacgt ttttccatgt ttatggactt tcgtttgatt tgttcgatga tgacgatttc
      181 atacgcgcgg cttccgccaa gatggaggaa attttcggat tcggaatcat tgaaaaagcc
      241 aagcattcgg gcgggcgttt ctatgaaggc tgctggctga tgggaacgga aaatgcccaa
      301 tacgggcgcg tccattacgg cggccaacgt gaaacgatgt tggttgaatt gacggcggta
      361 ggctgcaatg ctgcaaatat tggatgggaa tcaaggcttt ttgattttct gacaaatgcg
      421 attcgtccaa aaataacgcg cgttgacatt gcaaaagact ttttcaacgg agagtacagc
      481 ccgaatcagg caagagaaga ccgcaataaa ggtttgttta cgtgccatca cgtcaaaccg
      541 aagggcgaat gtttggggtc ggattgggaa gaagacgatg aagccaaaat gaccaaaggc
      601 aagacctatg gtatcggctc ccgtgaatcg tccaaatatg tccgcgtcta tgaaaaaggc
      661 aagcagttgg gcgataaaac aagcacatgg acgcgctttg aaattgaatt caaagcaaaa
      721 gacatcgtta tccctttcga agttttgcag aatccgggcg aatatttcgg cggcgcatat
      781 cccatttgcg aacgattcgc ccaaaaggca acgcgcatac acgcggttaa ggaagataag
      841 gttgtttcag ccgaccgctg ccttgaatgg gtaaaaaaac agttcggacg tgcggcaaac
      901 ggtctgaaat tcatttttcc cgaattggac aaagccaaac tgtttgaact gattgagccg
      961 agtcatcaca agctgcccaa gtctttggct ctcgaagcct acgactgcgc ctttttgaaa
     1021 tctcaagtca ttcatgaaca gcccgcattc aaaccgtaca atgaccctta ttatatgtac
     1081 gaatattacg agaatcttga aaaacagctt gaacagcaaa aacacgtcaa caatgaagaa
     1141 agctataaca acttcattta cgacaaattc gcaagactac cgatttcatg ggcttaaagt
     1201 gtctgcccga aagacgttta atcacacaag gaaaccaaaa aatgaacatc caacttcaag
     1261 gccacatcgt cggcgttaaa aaattcaacg gacaaatcga aggcaagagc ttcgactatt
     1321 gccgcctgat tgtcgccaca cccttagaca gctcccaagg caacgcattg ggcagctcta
     1381 ctactgaata cgatttcggc ggctctgcca atttcgagca gttccgaaac gcccaatttc
     1441 cgatcgaagc aaacctgaac gtggaaatcg tcactacggg caaaactcaa aaactgaaag
     1501 tcaccggttt tcaattcgtt aagaaaggct gattgaatgc agaaagtcta tgttgtccag
     1561 tccgtatcaa caggggactt tctgtatctc tctcctgaaa cgggcgacat cggacatacc
     1621 aaattaatta ccaatgccga ttatttctac gacttcgaag aagcggttaa cgcaggtttg
     1681 gaagaaatcg gcaaccaatg cgaatttgtc gtattcggat ttttgaaaga ctgattttcg
     1741 gatgttcggc ggtcgtctga aaaactctcc atccattacc gccaaacact tttt
//
LOCUS       DAXFHF010000126         1761 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXFHF010000126 DAXFHF010000000
VERSION     DAXFHF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.127"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(1..116)
                     /locus_tag="ACFIRM_002066"
                     /pseudo
     CDS             complement(1..116)
                     /locus_tag="ACFIRM_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(134..477)
                     /locus_tag="ACFIRM_002067"
                     /pseudo
     CDS             complement(134..477)
                     /locus_tag="ACFIRM_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(876..1277)
                     /locus_tag="ACFIRM_002068"
     CDS             complement(876..1277)
                     /locus_tag="ACFIRM_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029595.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1281..>1761)
                     /locus_tag="ACFIRM_002069"
     CDS             complement(1281..>1761)
                     /locus_tag="ACFIRM_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029596.1"
                     /translation="GYKKPTARHMQTKALGTVDEIGNTVQQVGKQTSGQKTSGGNPAI
                     DSDPYSPSSVSARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDF
                     NSLNVRNVQTRPNGTITGNLPDGRAVNVRNDSSGGEPTLEITISNNRKIKIRYGNTR"
BASE COUNT          544 a          349 c          266 g          602 t
ORIGIN      
        1 gcactgtctg taattgcccc aatccctact ccttgaaaac ccattaaaaa aggattaacc
       61 gcaccttgga cgatgccgcg aatatcatcc atacggttag cccaccaatt tttattggtt
      121 tattggtata tcgttaatca tttatttcaa aaaagttatt tattatcata ttatcaggga
      181 gcgggtcgtt aaaaaaactc cctcaaattc cattaaatta gtatttatcc agcacataaa
      241 cgaaccaaag tgttctttcc ctgagtgtaa atatttaaaa ttaaattcaa aataagtttt
      301 tttgcttaga aaatttaatg gtttttctat agtgaaaaaa aagcccttac tcgttttagt
      361 tcgagatact attttaaata agtttttatt ctcaataaaa taagaatatt catttactaa
      421 gttgttatga tgaaaaagta attttattaa aatgttttca taattttcta ggttcatttc
      481 tagctcaatt taaaatgatt tcattgttgc cgtaccaatg cgaaatttac catcgacaaa
      541 atttcccttc actgatacag gtaccccatc gcagagatgt aggtcggata tttgtatccg
      601 acaaaaatat ttgacgcgtt tatcgtttcc gaaatgccgc tgttagaaaa tgtcggattc
      661 gagaatccaa cctacggcta tatccgtaaa gtctgaccat tgttcaatgc ggaaggcgcg
      721 aatattgcgg acggaatgta gaaaccaata tacttaattg ccatgccatt ctcatcaaaa
      781 tacctgtgct gtttaaagtg cgtggttcat tacacgagta attttatttt caggctgcct
      841 gaaagaaaga taggcaactt attactcatc ttacgctagc tctcaataat tgaatacgtt
      901 gcactagaac caattatttc tatgaatgtt tgctcaaaca taaaaatata gtatttaaag
      961 aattctctat tcaaaggaaa ataactttct ttcgatatcc atgccgcata acgctcatca
     1021 agaagttcga aaaaagattg accaccattt tcatgaagca aagctctatc tgcatcttcc
     1081 gaaattaaat ccataaaagt atctataaaa tacgattcat caacaaccct aaaatgaata
     1141 acatcctcaa tagaaatttt tatggtttta cccgtatttc tataaccaac tgctataact
     1201 tctactttat caaagtttat tgctgcatta tcaaaataga aatagccagt tgggaaatct
     1261 aagcttttta atttcataat ttatcgtgta tttccatatc tgatttttat ttttcggtta
     1321 ttactaattg ttatttcaag tgttggttct ccaccactgc tatcattacg aacattaacc
     1381 gcacgcccat caggtaaatt gcccgttatc gttccattag gacgtgtttg tacatttcga
     1441 acattcagac tattaaaatc atttagtgcg tcattgtaat cccccacttt atcatactga
     1501 acagcgggac cttttgtttt actcctttga gtagtattgc tcaaaatgtc tttgatttgt
     1561 aaatcactgc gcaccttacc ggcttctata cgagctgaca cactactcgg gctatagggg
     1621 tcgctatcaa tcgcaggatt accaccgctg gttttttgtc cgctagtctg tttcccaacc
     1681 tgctgtactg tattgccaat ttcatctacc gtacctaacg ccttagtctg catatggcgg
     1741 gcagtaggtt ttttataacc g
//
LOCUS       DAXFHF010000127         1741 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXFHF010000127 DAXFHF010000000
VERSION     DAXFHF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.128"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            59..1000
                     /gene="mafA"
                     /locus_tag="ACFIRM_002070"
     CDS             59..1000
                     /gene="mafA"
                     /locus_tag="ACFIRM_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3029597.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSYPA
                     YDTTATTKSDALSGVTTSTSVLNAPAAALTKNSGHKGERSAGLSVNGTDDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            1004..>1741
                     /gene="mafB"
                     /locus_tag="ACFIRM_002071"
     CDS             1004..>1741
                     /gene="mafB"
                     /locus_tag="ACFIRM_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC3029598.1"
                     /translation="MKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEAL"
BASE COUNT          464 a          649 c          375 g          253 t
ORIGIN      
        1 tacaaaccct taaaatccat ttacacttaa acccgttcaa aacaaaacaa accctgccat
       61 gaaaaccctg ctcctcctca tccccctcgt actcaccgcc tgcggcacac tgaccggtat
      121 acccgcccac ggcggcggca aacgctttgc cgtcgaacaa gaactcgtcg ccgcatcgtc
      181 ccgcgccgcc gtcaaagaaa tggacttgtc cgccctgaaa ggacgcaaag ccgcccttta
      241 cgtctccgtt atgggcgacc aaggttcggg caacataagc ggcggacgct actctatcga
      301 cgcactgata cgcggcggct accacaacaa ccccgacagc gccacccgat acagctaccc
      361 cgcctatgac actaccgcca ccaccaaatc cgacgcgctc tccggcgtaa ccacttccac
      421 atcggtctta aacgcccccg ccgccgccct gacgaaaaac agcggacaca aaggcgaacg
      481 ctccgccgga ctgtccgtca acggcacgga cgactaccgc aacgaaaccc tgctcgccaa
      541 cccccgcgac gtttccttcc tgaccaacct catccaaacc gtcttctacc tgcgcggcat
      601 cgaagtcgta ccgcccgaat acgccgacac cgacgtattc gtaaccgtcg acgtattcgg
      661 caccgtccgc agccgtaccg aactgcacct ctacaacgcc gaaaccctta aagcccaaac
      721 caagctcgaa tatttcgccg tcgaccgcga cagccggaaa ctgctgattg cccctaaaac
      781 cgccgcctac gaatcccaat accaagaaca atacgccctc tggatgggac cttacagcgt
      841 cggcaaaacc gtcaaagcct cagaccgcct gatggtcgat ttctccgaca tcacccccta
      901 cggcgacaca accgcccaaa accgtcccga cttcaaacaa aacaacggta aaaaccccga
      961 tgtcggcaac gaagtcatcc gccgccgcaa aggaggataa accgtgaaac cgctgcgaag
     1021 actcatcaag ctccttgccg cctgtgccgt agcggcggcc gcactcatac agcccgccct
     1081 cgcggcggac ttggcgcaag acccgttcat taccgataac acccaacggc agcactacga
     1141 acccggcggc aaataccacc tcttcggcga cccgcgcggc agcgtttccg accgcaccgg
     1201 caaaatcaac gtcatccaag actataccca ccagatgggc aacctgctta tccaacaggc
     1261 aaacatcaac ggcacaatcg gctaccacac ccgcttttcc ggacacggac acgaagaaca
     1321 cgcccccttc gacaaccacg ccgccgacag cgcaagcgaa gaaaaaggca acgttgacga
     1381 cggctttacc gtgtaccggc tcaactggga aggacacgaa catcatcccg ccgatgccta
     1441 cgacggcccg aagggcggca attaccccaa acctacgggc gcacgagacg aatacaccta
     1501 tcacgtcaac ggcacagccc gcagtatcaa actcaatccg accgacaccc gcagcatccg
     1561 gcaacgcata tccgacaact acaacaacct cggcagcaat ttctccgacc gcgccgatga
     1621 agccaacaga aaaatgttcg agcacaatgc caagctcgac cgctggggca acagcatgga
     1681 gtttatcaac ggcgtcgccg ccggcgcgct caaccccttt atcagcgcgg gcgaagcctt
     1741 g
//
LOCUS       DAXFHF010000128         1728 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXFHF010000128 DAXFHF010000000
VERSION     DAXFHF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.129"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            244..528
                     /locus_tag="ACFIRM_002072"
     CDS             244..528
                     /locus_tag="ACFIRM_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3029599.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            529..1716
                     /locus_tag="ACFIRM_002073"
     CDS             529..1716
                     /locus_tag="ACFIRM_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC3029600.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
BASE COUNT          544 a          374 c          448 g          362 t
ORIGIN      
        1 taagcaactt tagccaaagg caggcgaagt accgccgggc ttcggtacct tgtgccaagg
       61 caggcgaagt accgcacttt gcgggcgaag ccgcaaacag ccgagaagcg cggggggggg
      121 attggcgata agcgcgaggg ggtgtcccca cagcgctgcc gcgccgcgaa tgcggagcaa
      181 tctttcagat taagaaacat ttgtttaatg aggcaaacgt gccttttaag aaagggagag
      241 caaatgaaat tgttggccgc attgattccg ctcttgatga gcgtcgcagg ccgtatattg
      301 actgcattag gcttgatggc tgttacctat gcgggtgtag atagattggc agcccatttt
      361 caacaggcga taacccatag cataacgggc gcgcctcaag caatgttaca gcttttctat
      421 ataagcggcg gtggtactgt tctaaacatt cttttcggcg caatcgcctt tattctgtcc
      481 tttaaacaaa tgacaaaact cgcaacctca atcgggaaga aaaaataaat ggcagaaatc
      541 tgtttgataa ccggcacgcc cggttcaggg aaaacattaa aaatggtttc catgatggca
      601 aacgatgaaa tgtttaagcc agatgaaaac ggcgtacgcc gtaaagtatt tacgaacatc
      661 aaaggtttga agataccgca cacccacata gaaacagacg caaagaagct gccgaaatca
      721 accgatgaac agctttcggc gcatgatatg tatgaatgga tcaagaagcc tgaaaacgtc
      781 ggcgcaatcg ttattgtcga tgaggcgcaa gacgtatggc ccgcacgctc cgcaggttcg
      841 aaaatccccg aaaacgtcca atggctgaac acacacaggc atcagggcat agatatattt
      901 gtattgacac aaggtcctaa actcttagat cagaacttgc gaacattggt taaaagacat
      961 taccacattg cggccaacaa aatgggtttg cgtaccctgc ttgaatggaa agtatgcgcg
     1021 gatgacccgg taaaaatggc atcaagtgca ttttccagta tctacacact ggataaaaaa
     1081 gtttatgact tgtacgaatc cgcagaaatt cacacggtaa acaaagtcaa gcgttcaaaa
     1141 tggttttatg cattgcccgt catcatatta ttgattccgc tatttgtcgg tttgtcttac
     1201 aaaatgttgg gcagttacgg aaaaaaacag gaagaacccg cagcacaaga atcggcggca
     1261 acagaacagc aggcagtact tccggataaa acagaaggag aatcggtgaa taacggaaac
     1321 cttacggcag atatgtttgt tccgacattg cccgaaaaac ccgaaagcaa gccgatttat
     1381 aacggtgtaa ggcaggtaag gacctttgaa tatatagcag gctgtataga aggcggaaga
     1441 accggatgca cctgctattc gcatcaaggg acggcattga aagaagtgac ggagttgatg
     1501 tgcaaggact atgtaaaaaa cggcttgccg tttaacccat acaaagaaga aagccaaggg
     1561 caggaagttc agcaaagcgc gcagcaacat tcggacaggg cgcaagttgc caccttgggc
     1621 ggaaaaccgc agcagaacct aatgtacgac aattgggaag aacgcgggaa accgtttgaa
     1681 ggaatcggcg ggggcgtggt cggatcggca aactgaagaa aacggcaa
//
LOCUS       DAXFHF010000129         1539 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXFHF010000129 DAXFHF010000000
VERSION     DAXFHF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.131"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..354
                     /locus_tag="ACFIRM_002074"
     CDS             <1..354
                     /locus_tag="ACFIRM_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC3029601.1"
                     /translation="VKNTGYKTPAVRHMQTVDGEMAGGNKPVVKSIRPTTRDELRQAL
                     QEQGFRRTGSDAAQYETWKGPDGVKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEG
                     NPLPNNHHHSGYFVK"
     gene            351..668
                     /locus_tag="ACFIRM_002075"
     CDS             351..668
                     /locus_tag="ACFIRM_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029602.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            738..1073
                     /locus_tag="ACFIRM_002076"
                     /pseudo
     CDS             738..1073
                     /locus_tag="ACFIRM_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1047..1331
                     /locus_tag="ACFIRM_002077"
     CDS             1047..1331
                     /locus_tag="ACFIRM_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029603.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNINKVEIHYIENTRTGEKTDFKFKD"
     gene            1334..>1539
                     /locus_tag="ACFIRM_002078"
     CDS             1334..>1539
                     /locus_tag="ACFIRM_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029604.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILR"
BASE COUNT          558 a          259 c          311 g          411 t
ORIGIN      
        1 gttaaaaata ccggctataa aacacctgct gttcgccata tgcagactgt agatggggag
       61 atggcaggag gaaacaagcc agttgttaaa tctattagac caactacgcg agatgaatta
      121 cgtcaagcat tgcaagaaca aggttttaga cgtactggtt cagatgcggc tcaatatgaa
      181 acatggaaag gtcctgatgg cgtgaaaata gatattcgtc caaatggaga ggttataaga
      241 acccaaagag tgccgcgaac cgatggtgta cagggaaaat atccgcaacg acaagattat
      301 gaaggcaatc cattgccaaa taatcatcat cattctggat attttgtcaa atgaaaaaaa
      361 atatttttca caatgtaagc ctttatgaaa taatcttttc cgataatgga aataccctta
      421 cattatcttt tacagataca attgaaggta attatttcgg atatatcaaa tgcagtaata
      481 ttttgaattt taaattagat acaaataatt tcgtagatta tgaggataag gaagatagct
      541 tgtttccctt gtttataccc gaaatagagc tatataaata ccaattttat agtgaaatta
      601 ttattgatgt agggattatt ataaaaatat ctgctgaaac aattaatttt gagccactgg
      661 gaaaatagta actgctttcc cagcagccgt agcaactgta tttttacccg acggggtaaa
      721 aatacagttg ctacatctct gcgcagcaga ctctacaagg tattaataat tcaggaaaat
      781 taagtccgga agcacaactt gccgccgtga gcctattaca ggacagtgct tttgcggtaa
      841 aagacggtat caactctgcc agacaatggg ctgatgccca tccgaacatt acagctactg
      901 cccaaactgc ccttgccgta gcagaggccg caggtacggt ttggagaggt aaaaaagtag
      961 aacttaaccc gactaaatgg gattgagtta aagataccgg ctatgaaaaa cctactgccc
     1021 gccatatgca gactgtagac agggaaatgg ctgggggaaa caaatcatta aaaataggga
     1081 cacaatctgt tgaaaaatca accggtcgta caatacctaa taatttaaag gaacaattag
     1141 caatggaaga agttaaggca aacccacagg gcaaaactcc tgcgagaata cctcctatgt
     1201 ccgatactaa aaatggttgg ttggcaaaag acggatgggt taagcgcgtt caaaatataa
     1261 acaaagttga gatacattac attgaaaata caagaaccgg tgagaaaaca gatttcaagt
     1321 ttaaggatta gccatgtttt tagatgatgt aaatgttttt ttagatgatt taaataccaa
     1381 tccaatcact gacgaatggt atatgtccaa ttttgccgat aaacatatta aaattttgga
     1441 aagttacgaa gcctttgata ttctaaaaca atttgttgat tacatgattg aagaacatga
     1501 tgaaaaatca gaatatgaaa tcatggaaat attgagaca
//
LOCUS       DAXFHF010000130         1396 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXFHF010000130 DAXFHF010000000
VERSION     DAXFHF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.133"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(46..1029)
                     /gene="porB"
                     /locus_tag="ACFIRM_002079"
     CDS             complement(46..1029)
                     /gene="porB"
                     /locus_tag="ACFIRM_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_218015349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IA"
                     /protein_id="HFC3029605.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHHGAQA
                     DRVKTATEIADLGSKIGFKGQEDLGNGLKAIWQLEQKAYVSGTDTGWGNRQSFIGLKG
                     GFGKVRVGRLNSVLKDTGGFNPWEGKSYYLGLSNIAQPEERHVSVRYDSPEFAGFSGS
                     VQYVPNDNSGKNHSESYHAGFNYKNSGFFVQYAGFYKRHSYTTEKHQVHRLVGGYDHD
                     ALYASVAVQQQDAKLTWSNDNSHNSQTEVAATAAYRFGNVTPRVSYAHGFKGSVYDAD
                     NDNTYDQVVVGAEYDFSKRTSALVSAGWLQRGKGTEKFVATVGGVGLRHKF"
BASE COUNT          315 a          321 c          375 g          385 t
ORIGIN      
        1 agcctgtctg acgacaggct ttttgttgat accgatcttt gcagattaga atttgtggcg
       61 cagaccgaca ccgccgacag tcgctacgaa tttttctgtg cctttgcctc tttgcaacca
      121 accggcagaa accaaggcag aagtgcgttt ggagaagtcg tattccgcac cgacaaccac
      181 ttggtcgtaa gtattgtcgt tatctgcatc ataaaccgaa cctttgaagc cgtgggcgta
      241 agaaacgcgg ggcgttacgt tgccgaagcg gtatgccgcg gtagcggcaa cttcggtttg
      301 agagttgtgc gaattatcgt tggaccaagt caatttcgcg tcttgttgct gtacggctac
      361 ggaagcgtac agggcatcat ggtcgtaacc gccgaccaaa cggtgaacct ggtgtttctc
      421 agtcgtgtaa ctatgtcttt tatagaagcc ggcatattgc acgaagaagc cgctgttttt
      481 gtagttgaag ccagcatggt aagattcgct gtgatttttg cccgaattgt cgttaggcac
      541 gtattgcacg ctgccgctga agccggcaaa ttcgggagaa tcgtagcgta cggaaacgtg
      601 gcgttcttcg ggttgggcaa tgttgcttaa acccaaatag tagcttttac cctcccaagg
      661 attgaagccg ccggtgtctt tcaggacgct gttcaaacgg ccgacgcgca ctttaccgaa
      721 gccgcctttc aaaccgatga aggattggcg gttgccccag cctgtgtcag taccgctgac
      781 gtaggctttt tgttccaact gccaaatggc tttcaggccg ttgccgaggt cttcttgacc
      841 tttaaagccg attttcgaac ccaaatcagc gatttcggta gcggttttaa cgcgatccgc
      901 ctgagctcca tggtgagcta cggagcggga agtttctacg ccggctttga tggtgccgta
      961 cagggtaacg tcagccattg ctgcaacagg aagggctgcc aaagtcaggg caatcaggga
     1021 ttttttcatt gctgtattcc ttttttggtt aagaaattta agcaggccgg gttttccaag
     1081 ccgcttagct ttgcatttac cgccgagggg ctcggcgatt acatgtctga aactatagat
     1141 gttcctgtct gaaaaaacaa acttctgcct gtttttgtgt ggaatccgat gtgcattttg
     1201 aagggcggat agagatatta cgggtatttt ttgttttgta acatatggtt attttaaatt
     1261 tttaagattt gcatttttac aacacttact cgggagggta ttggagggca ttgcaaaccg
     1321 ggggttatag cggattaaca aaaaccggta cggcgttgcc cccgccccgg cccaaaggga
     1381 acggttccct aaggcg
//
LOCUS       DAXFHF010000131         1366 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXFHF010000131 DAXFHF010000000
VERSION     DAXFHF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.134"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(69..1265)
                     /locus_tag="ACFIRM_002080"
     CDS             complement(69..1265)
                     /locus_tag="ACFIRM_002080"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3029606.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT          263 a          340 c          432 g          331 t
ORIGIN      
        1 ggtttaaatt taatccacta taaaaaactg cctccgtttc cggaggcagt tcgcgtgagg
       61 ataaaacctt acagaccgct caaacgggcg gtatcgtggg caatcatcag ctcttcgttg
      121 gtcggaatga ccacggcaac ggctttgctg tcggcagtgg taatcacgcc ggcgttgccg
      181 aagcgggctt tcaggttggc ttcttggtcg atgttcagac cgaggaagcc caagtagccg
      241 attacgcgtt cgcggatgat gtcggagttt tcgccgatac cgccggtaaa gaccagtgcg
      301 tccaagccgc ctgcggcaac cgccatactg ccgatgtatt tggcaaggcg gtagataaac
      361 atatccaagg ccaatttcgc gcctttatgc cccttggcgg cttcttcttc aatggtgcgg
      421 cagtcgttgg acaggccgga aatgccgagc aggccggatt ttttgttcag catgtcggtg
      481 atttgggcaa tggtcatatt ggcgttttcg gcgaggaagc cgaatacgga aggatcgatg
      541 tcgccgctgc gcgtacccat taccagccct tccagcgggg tcaggcccat actggtgtcg
      601 cgcgattctc cgttggcgac ggcggtgatg gatgcgccgt tgcccaagtg ggcaatgacc
      661 atacgcaggt cttttttgtc tttgccgagg aagcgcgcgg tttcgtcggc aacgaagcgg
      721 tagctggtac cgtgcgcgcc gtagcggcgc aggccgtatt tttcatacaa ctcctgcgga
      781 acggcgtatt tgtaggcgac ttcgggcatg gtttggtgga aggaggtatc gaatacgacg
      841 acgttgggca ggcctttgaa aatgctttgc gcggcacgca ggcccaagag gtgggcggga
      901 ttgtgcagag gagccagcgg gatgcatttt tcgatgccgg cgatgacttc gtcgtcaacg
      961 aggatggatt cgttgtacag ttcgccgccg ctgacgacgc ggtggccgat ggcgccgatg
     1021 cggctgtcga ggccgtgggc ttcgagttct tccatcaggg cttcgaccgc gccggtgtgg
     1081 tcggggtgtg cggacagatc gactttgtgt ttttcgccgt ttactttgaa tgtaatgtag
     1141 gcatcgggca ggttgagttt ttcggcaagg cagctcagca ggacttcgcc gctgccgtta
     1201 tccaggaccg cgcctttgag ggacgagctg ccgcagttca aaaccaagat caatttttgg
     1261 gacattttct tactccggaa agtttcagac ggcattggaa tcggacacgg atactaatcg
     1321 gattcgtgcc gaatccgctt tgctttcctt gacgggaaag tagtgg
//
LOCUS       DAXFHF010000132         1356 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXFHF010000132 DAXFHF010000000
VERSION     DAXFHF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.135"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..435
                     /locus_tag="ACFIRM_002081"
     CDS             <1..435
                     /locus_tag="ACFIRM_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3029607.1"
                     /translation="SISYKNFSTVKPKVIAKGTINGKTFRDVNQSAKIGSPDSPTLIA
                     QRVNAKIQADGKPRPNATVANSHAEIGVIQQAYNAGETKGASMTMTVSGKDVCGYCKG
                     DIAAAAQASGLKSLTVNATDNVTGKNKTYYWTPGMKSIKERK"
     gene            432..848
                     /locus_tag="ACFIRM_002082"
     CDS             432..848
                     /locus_tag="ACFIRM_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029608.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTENYSGNTME
                     ILGDVYPIEHISKNISIIQDIISEFIMKNKPITIMI"
     gene            951..1356
                     /locus_tag="ACFIRM_002083"
                     /pseudo
     CDS             951..1356
                     /locus_tag="ACFIRM_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
BASE COUNT          482 a          265 c          252 g          357 t
ORIGIN      
        1 tcgattagct ataaaaactt ttctactgtc aaaccaaaag taattgccaa aggaacgatt
       61 aatggaaaga cttttagaga tgttaatcaa agtgcaaaaa ttggatcgcc tgattcacct
      121 acattaatag cccaacgagt taatgcaaaa atccaagccg acggtaaacc tcgtccgaat
      181 gcaaccgttg ctaattcaca tgctgaaatt ggtgttatcc agcaggctta taatgcaggt
      241 gaaacgaaag gggcatctat gacaatgaca gtttcaggaa aagatgtttg cggttattgt
      301 aaaggagata ttgcagctgc tgctcaagct tctggactga aatcactgac tgttaatgcg
      361 acagataatg taacaggaaa aaataaaact tactattgga cgcctggtat gaaatcaatt
      421 aaggagagaa aatgaaaaca tcaacaattg tttttggtgg attttttatt acagataatg
      481 gagaacgaat ccaaatccct attttagaaa atcctaacat taaagaaata aataactttt
      541 tttctgtatc aaattttgaa aaaaaagccg gagtccttgt tttcagaatt attcctgagc
      601 ctgaatttgg caatactgaa ttaactatct attttgaaaa aggatattat ttaccaataa
      661 ttcaaacaat tttagaagat ggagatattg aagtaaaaaa tcttaaaacg gaaaattata
      721 gtggaaatac aatggaaatt ttgggagacg tctaccctat tgagcatatc tctaaaaata
      781 tttctataat tcaagatata atttctgaat ttattatgaa aaataaacca atcacaataa
      841 tgatttaatt gaaaagttca acaatcaaaa ggtcgcctga aatcgtgttt aattttcaga
      901 cgacctattt ccttcatttg aaacaggata ttgaaaacta agttcttcaa aaatcctaca
      961 cctgctcctt ccacggcagc accttggtca aaacggcaga cggctacaaa gccattgccc
     1021 gtatccgagc cggcgagagc gtcctctcca aggacgaggc aagcgggaaa acaggataca
     1081 aacccgttac cgcccgatac ggtaatccgt atcaagaaac cgtttacatt aaagtttcag
     1141 acggcatcgg caacagccaa accctgattt ccaaccgcat ccatccgttt tattcgggcg
     1201 gcaaatggat taaagcggaa gatttgaaag cgggaagcag gctgtttgcc gaaagcggca
     1261 aaacccaaac cgtccgcaac atcgttgtca aaccaaaacc gctcaaagcc tacaatctga
     1321 ccgtcgccga ctggcatact tacttcgtca agggca
//
LOCUS       DAXFHF010000133         1330 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXFHF010000133 DAXFHF010000000
VERSION     DAXFHF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.136"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..404
                     /gene="mafB"
                     /locus_tag="ACFIRM_002084"
     CDS             <1..404
                     /gene="mafB"
                     /locus_tag="ACFIRM_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3029609.1"
                     /translation="KPAARPMQTVDGEMAGGNKPIKSLPNSAAEKRKQSFKKFSSNWS
                     SASFDSVHKTLTPNAPGILSPDKVKTRYTSLDGKITIIKDNENNYFRIHDNSRKQYLD
                     SNGNAVKTGNLQGKQAKDYLQQQTHIRNLDK"
     gene            401..757
                     /locus_tag="ACFIRM_002085"
     CDS             401..757
                     /locus_tag="ACFIRM_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC3029610.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILTVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            931..1305
                     /locus_tag="ACFIRM_002086"
     CDS             931..1305
                     /locus_tag="ACFIRM_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3029611.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFKNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT          500 a          184 c          240 g          406 t
ORIGIN      
        1 aaaaacctgc tgcccgacct atgcagactg ttgacgggga aatggcaggt gggaataaac
       61 ctattaaatc tttaccaaac agcgccgctg aaaaaagaaa acaaagtttt aagaagttta
      121 gtagcaactg gagttcagca agttttgatt cagtgcacaa aacactaact cccaatgcac
      181 ctggtatttt aagtcctgat aaagttaaaa ctcgatacac tagtttagat ggaaaaatta
      241 caattataaa agataacgaa aacaactatt ttagaatcca tgataattca cgaaaacagt
      301 atcttgattc aaatggtaat gctgtgaaaa ccggtaattt acaaggtaag caagcaaaag
      361 attatttaca acaacaaact catatcagga acttagacaa atgaatgaac acaacctgtt
      421 aattttctgt ttaaaagaca atgtttcaat tagtgaatat actgaaatgg ttgattgggc
      481 ttatgaaaac attcaatctg aaacagttgt agaaattacg gaaaatcaaa ttattgaata
      541 tcaaaatcgt ggattatgga gacttgtttc tgaaattacc gataattggt tatttggacc
      601 aagtgagggg gattggctaa tagataagga aagtattttg actgtaaaag aaaaattaca
      661 aaattcagat ttttctacag agcccttagt gaaaaatatt attcatgtac ttgaatatgc
      721 tataaaaaat gaaaaaacag taatttttca tttttgaaac taatctagtt tttagtaata
      781 ttgatgcaga gcaagcagca ttagatgccg caaacatggg gagaagctat tcaatttaga
      841 attaaaaaaa caaagtgaaa atggactagc accaccaaat tggtctaccc agtttcctaa
      901 tggtagtatt tatgatacta aggtaacgaa atgattattc aaaatgaatt taatttatat
      961 cctagtaata tgcttcctga agggttttgt tatcctgaaa agtatgttcg tatctctaac
     1021 gatacatctt taatacctta tattcagcca cataattttc actggtggtt tgagaattat
     1081 ggaacagaag gggcagaagt agcttatata tttaaaaatt ccatcctgcc tgatttaaat
     1141 cttatcccat tcgctagtaa tggagaatgg gaagcttatt ttgatggtaa tgatgtaaca
     1201 ggaaatccta gggttattgt tattaattta gataatatag aaaaccatga attttttaat
     1261 agttttgaag agtggcttga attagcaatt aaggatactt ggtaagcagc tatctataaa
     1321 gagatgaggc
//
LOCUS       DAXFHF010000134         1251 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXFHF010000134 DAXFHF010000000
VERSION     DAXFHF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.137"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(278..688)
                     /locus_tag="ACFIRM_002087"
     CDS             complement(278..688)
                     /locus_tag="ACFIRM_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029612.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(692..>1251)
                     /locus_tag="ACFIRM_002088"
     CDS             complement(692..>1251)
                     /locus_tag="ACFIRM_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC3029613.1"
                     /translation="TVWRGKKVELNPTKWDWVKNTGYKKPAARHMQTLDGEMAGGNKP
                     AKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHKPDA
                     SSLDKIDSKSLFKSDVDVEKATLDAARYADKHNLWDSQNKAKVPTNDVVGYTDGKPTH
                     IINIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          369 a          233 c          196 g          453 t
ORIGIN      
        1 aaaatgtcgg attcgagaat ccgacctacg gttgctgaac aatgttttgt agaacttgct
       61 caaaatccat gtttcgccat tttcctttat atatacctaa tgcagcacgg gttacgattt
      121 cgtttaaatc ataaatagcc atttacgtcc tctgctaatt ttcagcgagt agattgggtt
      181 accaaaccca acaaaaaagc agcctgaaaa atttcagaat gcttgtttta tgttttttat
      241 caatgattta ggttttaatc cagcctgcgc ttgctagtta aacagtattt acataaaaat
      301 cttgcactga acaaacaagc gtatcatgtt catcatgaaa tgatgggcat aacgttaatc
      361 tacaaatgtt tacatttaaa gatatttttt cagtttcaat atttttaatt tgtaaggtta
      421 atacatgagg atatttgtga tatagctgat tttttaattg ttcgttgtct aatatagcaa
      481 aaggtaattt tgcacacaaa taaccaataa ctttgttttt atgaaaaaaa actatccaat
      541 tttcaattcc ttcatcccaa tcaaaagtaa aattcgttcc ttctatgata aatttagcac
      601 aagatattaa atctaactta acccaagtgg atttatacag actcaatgct gatgaagtta
      661 taatattatc taagttataa attttcataa atcaacgttc cttacctggt gttccatgca
      721 caggtctaat cccatcttta ttcggctttc tacgataaat gttaattata tgtgtcggtt
      781 ttccgtctgt atagccaact acgtcgttcg ttggaacttt tgccttattt tgactatccc
      841 ataaattatg tttatctgca tatctagcag catctaatgt agctttttcc acatcaacat
      901 ctgatttaaa taaacttttg ctatctatct tgtctagact agatgcatca ggtttgtgtc
      961 gtaccatact gagagggtca gtaatatatt tgccatctgt aatagctgat ccaccattat
     1021 aggtagtatg cgtataattt ttttgtggaa cgagactatt ttgcaaagat tgttgtgtag
     1081 aaacgccagc tttgccatta ggagtagctt gcttcgctgg cttattccca cctgccatct
     1141 ccccatctaa agtctgcata tggcgggcag caggtttttt atagccggta tttttaaccc
     1201 aatcccattt agtcgggtta agttctactt ttttacctct ccaaaccgta g
//
LOCUS       DAXFHF010000135         1234 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXFHF010000135 DAXFHF010000000
VERSION     DAXFHF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.139"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(53..976)
                     /gene="lpxC"
                     /locus_tag="ACFIRM_002089"
     CDS             complement(53..976)
                     /gene="lpxC"
                     /locus_tag="ACFIRM_002089"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC3029614.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          293 a          299 c          347 g          295 t
ORIGIN      
        1 cgcgcgggaa tgacggcatc tgtttgacgg catttgtttg gaaaaggggg cgttatccgc
       61 aatttctgat gttcagctcg tgaaatgcgt cgggcaaatc atcgctgtcg gcaaattcca
      121 cccattcgta agccgtttcg tctgccaaaa ctgcgcgcaa aagggcgttg ttgatggcgt
      181 gtcccgattt gtagccttca aacgcgccga caatcggatg tccgacgata tacaaatcgc
      241 cgatggcgtc gaggattttg tggcgcacaa actcatcggg atagcgcaag ccttcaggat
      301 tcaggacatc cgtgtcgcca atcacgatgg cgttgttcaa attgccgcct aagccgagat
      361 tgtgggcgcg catcatttcc acttcgtgca taaagccgaa agtgcgcgcg cgcgcgattt
      421 cgccgatgta ggatttgccg gcgaaatcaa tttcaaacgt aggcgggctg cggttgaaaa
      481 caggatggtc gaattcgatg gtcagcgtta ccttaaaacc gtcatacggc gtaaatcgga
      541 cccatttgcc cgcttctttg atttcgacgg gtttgaggat tttcaaaaaa cgcttttgcg
      601 ccttttggtc tacaacgccc gcatcttgca ggaggtaaat aaacggcagg ctggagccgt
      661 ccataatcgg gatttcgggc gcgttcaact caatcagcgc attgtcgatg ccgtaggcgg
      721 acagtgcgga catgatgtgt tcgatggtgc cgacgcgcag gcctttgtcg gtaacgatgg
      781 tggatgaaag gcgggtatcg ttgatcaaat aagggttcag cttgatttgt tcgcccatct
      841 cgccgtccaa atcggtgcgg cggaaggaaa tcccgctgtt ttcaggcgcg gggtgcaggg
      901 tcagcgcgac gcgttcgccc gaatgcagcc cgactccggt aacgccgatg gatttcgcca
      961 aagttctttg cagcataaac cgcttcctta tcaatggggt aagtttcgga ataatacgat
     1021 aaaaccggaa aaacaggcta tgtttttcaa tagtatttgc caatgtatcc gtttccaaca
     1081 cgtaagccgc atgaaaatgt atggcggatt aacgaaaatc aggacaaggc ggcgggccgc
     1141 aggcagtacg gatagtacga aaaggcgagg caacgccgta ctggtttaaa tttaattcac
     1201 tatacatccc gtcattcccg cgcgggcggg aatc
//
LOCUS       DAXFHF010000136         1220 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXFHF010000136 DAXFHF010000000
VERSION     DAXFHF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.140"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            358..1155
                     /gene="truA"
                     /locus_tag="ACFIRM_002090"
     CDS             358..1155
                     /gene="truA"
                     /locus_tag="ACFIRM_002090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC3029615.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHATAQVVHFDTAAVRPAQAWIRGVNAHLPEGIAVLHARQVA
                     PGFHARFDASGRHYRYLLESAPVRSPLLKNRAGWTHLELDIGPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          271 a          417 c          302 g          230 t
ORIGIN      
        1 tttgtcgcta tgatacggta attccgatat acaagggcgg gcttcagccc gccaaatcca
       61 ccaactccgc cgaatccgcc gccgtcattc ccgcgcaggc gggaatccag acctgtcggt
      121 gcggaaactt atcggataaa acggtttctt agattccaca tcctagattc ccgcctgcgc
      181 gggaatgacg gttcgggcac tcctgacagg gtgaattcac aggatagcga ttcgtagcaa
      241 ctgcatccaa cccccgaaca actccccaaa caacgccgcg cgccccgggc gtttgccgtt
      301 tccctgcaaa atctgcgata caatgcagtc tgaacattta tccgaatccc aaacccgatg
      361 gataccgcac aaaaacaacg ctgggcaata accctatcct acgacggcag ccgcttttac
      421 ggctggcaga aacaggcggg cggcgtaccg accgttcagg cggcattgga aaccgcactc
      481 gcccgaatcg caggagaatc tgtcgccacc acagttgccg gcaggaccga caccggcgtt
      541 catgcgactg cccaagtcgt tcatttcgat actgccgccg tacgtcccgc tcaggcatgg
      601 atacgcggcg taaacgccca cctgcccgaa gggatagccg ttttgcacgc ccgacaggtc
      661 gcacccggat ttcacgcccg cttcgacgca tccggacggc actaccgcta cctgctcgaa
      721 tccgcccccg tccgttcccc cctgctcaaa aacagggcgg gctggacaca cctcgagctc
      781 gacatcggac cgatgcgccg ggctgccgcc ttattgatcg gcgaacaaga cttctccagc
      841 ttccgcgccg ccgggtgcca ggcaaaatcc cccgtcaaaa ccatctaccg cgccgacctt
      901 acccaaagcg cgggactcgt gcgcctcgat ttgcacggca acgccttttt gcaccacatg
      961 gtacgcaaca tcatgggcgc gctcgtttat gtcggcagcg gcaggctcag cgtcgaaggc
     1021 ttcgccgcac tgattcaaga acgcagccgc ctcaaagccc caccgacctt tatgcccgac
     1081 gggctttacc tgaccggcgt cgactatccc gaggcatacg gcatcatccg cccccaaatc
     1141 cccgaatggc tttaaaacat tcttgtcgcg gagattttga aatcggacaa actgtcaggc
     1201 aatctttttc aatgttggca
//
LOCUS       DAXFHF010000137         1150 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXFHF010000137 DAXFHF010000000
VERSION     DAXFHF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.144"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            83..427
                     /locus_tag="ACFIRM_002091"
     CDS             83..427
                     /locus_tag="ACFIRM_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC3029616.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            381..1034
                     /locus_tag="ACFIRM_002092"
                     /pseudo
     CDS             381..1034
                     /locus_tag="ACFIRM_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          348 a          216 c          262 g          324 t
ORIGIN      
        1 gaaataattc ccgtatttta atgtcgtatt gttcctgtat atttagtgat gtgataagta
       61 tagtttattt tttgaggttt ttatgaattt accttttcgg gcaatggtgt ctgatttagg
      121 tggcacactt ttaacacctg aacatttagt gggtgatctt accattgata ctctgagagt
      181 attagagcaa aaaggcgtag atattatttt ggcaacagga cgcaatcata ccgatatgtc
      241 atccattctt ggaaaaatcg gtgcggaacg tgcggttatg attacatcaa acggtgctcg
      301 tgtacgggat ttacagggta atttgcttta tagcaatagt ttgcctgaag aattagttct
      361 cgagctttac aaaacatcat atgtgggaaa tcttattcag ataccaagat tttgtcgcca
      421 tcaataaacc tcaaggcata tccgtccacc gttccggcgg cgaagtcagt ctgacggcaa
      481 cacttgcggc acagttgggc gtagaaaagg tatggttgct gcacaggttg gacaagcagg
      541 ccggaggcat attgctgttt gcccttaatc cccaaagtgc cgctgttctt gccgcacagt
      601 ttgccgaaag gaaaatgaag aaaacctatt tggctttgtc cgaccgcaaa ccgtccaaaa
      661 aacaaggatg gataaaaggc ggcatggaaa aatccaggcg cgggatgtgg aagctgacgc
      721 gcaatatgga aaatattgcc gttacccggt ttttcagtat ccgcatcagt gaaaaaatgc
      781 ggctgttcat ccttgagcca catacgggta agacacatca gttgagggtg gtaatgaaga
      841 gtctgggcag ctctatattt ggggacagcc tgtatggagg aacagaatcc gaaactatgt
      901 tcctgtatgc gtgaaaaata caatttacct atcaacaaca agaaatcgaa attaccgcac
      961 ctttgaaaaa tgcatggcag gccgaaaata tctctcatgc actggaagaa ttttatatgg
     1021 caaaagcagg ttaatagata taaatcaatt aaaacaatat gataaatatt tttataaaat
     1081 tttttcaaaa aacggttgac cgcaaatctc tttaagcgta tagttcagtt cgtccgtcgc
     1141 tgccgaagcg
//
LOCUS       DAXFHF010000138         1044 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXFHF010000138 DAXFHF010000000
VERSION     DAXFHF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.147"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..189
                     /locus_tag="ACFIRM_002093"
     CDS             <1..189
                     /locus_tag="ACFIRM_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029617.1"
                     /translation="PTLSPFIYPHYKNMKKPLLALSLAAVSAVTVADIPESNGKGEYK
                     VFTAYVKGKATAGNLKEK"
     gene            231..527
                     /locus_tag="ACFIRM_002094"
     CDS             231..527
                     /locus_tag="ACFIRM_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029618.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            524..763
                     /locus_tag="ACFIRM_002095"
     CDS             524..763
                     /locus_tag="ACFIRM_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029619.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT          307 a          291 c          213 g          233 t
ORIGIN      
        1 cccacacttt ctccatttat ttatcctcat tataaaaaca tgaaaaaacc cctgcttgca
       61 ctctcacttg cagccgtgtc cgccgttacg gtagccgata ttcccgagtc taacggcaaa
      121 ggcgaataca aagtctttac cgcttatgtt aaaggcaaag ccactgccgg caatttgaaa
      181 gaaaaataac gcacaccgtc catcggtttt cgttcaacga acgccacgat atgcgtttta
      241 aggcagagta cgcccgaagc aaaaacgatg cagacgacaa aagagggaat gcacacatcg
      301 ctaacggcgc aataggcaaa atggctgccg tgtatgccgg ttatacctac acccaaccgg
      361 gcggcgaatc cacacgtttc cgtggcggtt tggagcgtgt ccaagttaaa cgttatctgg
      421 gagaaaaatg ctgcggctac gcctgtccaa aaagcgaacg cttcaccctg caagcagatg
      481 gtgaaatccg tcaaaacctt ggcgaaggct ggtttctgaa gctatgagcg gggtggctgt
      541 ctatctccgc aacaaaaaca aaatagcaat tgcctcccaa gatgccaacc taaactccaa
      601 aggccggttc gtcagcagcg gtctgaatgt gggcaaacaa ctgaccggca gtctcggcgt
      661 agagtttgac ccatactacc gccaccgcgc aatccgcaag tctgccgaat ttgtcccgaa
      721 cactaccaaa accaaaacag acagcgaaaa gtttaacgag tagggcttcc gcgtagccgc
      781 aacgttctaa ttcccttacg tataaaaagc agcataaaaa tgctgccttt tcccatttcc
      841 aagggaagta ttccgtagtg cagcttacct cattgtttca cacaaaccga aaatgccgtc
      901 ttaaaaccaa ttttcagacg gcatttgtcc cttaaaacac gttttttcaa gcgcgaccat
      961 accaaacacc cacaggttgc cttccaccct tcgtacaacc tttgctccat caaacatcca
     1021 cttcctaaat actgatgtat actg
//
LOCUS       DAXFHF010000139         1038 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXFHF010000139 DAXFHF010000000
VERSION     DAXFHF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.148"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            120..611
                     /locus_tag="ACFIRM_002096"
     CDS             120..611
                     /locus_tag="ACFIRM_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC3029620.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEGHIGRMTGKLKAAKARNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEERKNL
                     QIR"
     gene            663..878
                     /locus_tag="ACFIRM_002097"
     CDS             663..878
                     /locus_tag="ACFIRM_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029621.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEDYIDEIRDKVDEADT
                     SLEFLKDVINDGISDLKNVIEVFEDGV"
BASE COUNT          357 a          214 c          266 g          201 t
ORIGIN      
        1 gaacagggct gcatcaacat caccatcggc agcgacgacc tattcatcaa catcgaacag
       61 gcatacgcca tacacgccgc gcttggcgaa gcggttgccg aatatgaggg aggggcacaa
      121 tgaccgccct cacactctac cgatgcgcgg cagacgtaca ggcggcgctg gattactact
      181 ttgacagcga aaccgagcgc gaagacacgc tggaagccgt tatcgggcag ttcgaggtca
      241 aagcgcaatc cgttattgct tatattaaaa accaagaaat cacggaaaaa atgcttgaag
      301 ggcatatcgg gcggatgacc gggaagctca aggcggcaaa agcgcggaat caaagcctga
      361 aagactactt ggcgcgcaat atgcaggcgg cgggcattac cgaaatcaaa gcggacgacg
      421 gcacttttaa agcctcgttc cgcaaatccg aagccgtcgt gatcttagac gaagcacaaa
      481 tccccgccga atttatgcgt gaggccgtca aaaccgaacc ggacaaaacc gccatcagaa
      541 aagcgattga aagcggtcgg caagtagcag gcgcgaagat tgaagagcgg aagaatttgc
      601 agattagata aaccgtagaa gatgttgacg acagcatccc attttgagtt aaggagcaaa
      661 aaatgagcta tttggaagat gtaaaaaacg cattaagggt aatagataac ttatgcaaag
      721 aagcattaaa agagcctgaa tcgttagagg attatataga cgaaattagg gataaagtag
      781 acgaagcgga tacctctttg gaatttctaa aggatgtaat aaatgatggt attagcgatt
      841 taaaaaatgt aattgaggtg tttgaagatg gcgtttgata ttaatggaac aagacatgaa
      901 ctatcaattt aaattcggcg acctcgtgag agatacagct ttatgattgg gcattgatag
      961 aacggcatca aaaaatcgag aaaacaaaat gcaaacagta gcaacaaaac cgacggcaaa
     1021 acagatgctt gccgccaa
//
LOCUS       DAXFHF010000140         1017 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXFHF010000140 DAXFHF010000000
VERSION     DAXFHF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.149"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>1017
                     /gene="tuf"
                     /locus_tag="ACFIRM_002098"
     CDS             <1..>1017
                     /gene="tuf"
                     /locus_tag="ACFIRM_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC3029622.1"
                     /translation="IDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMI
                     TGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVE
                     MEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALDSYIPTPERAVDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLD
                     EGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFA
                     NYRPQFYFRTTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRT
                     VGAGV"
BASE COUNT          262 a          287 c          267 g          201 t
ORIGIN      
        1 atcgacaacg cacccgaaga aaaagcacgc ggtattacca ttaacacctc gcacgtagaa
       61 tacgaaaccg aaacccgcca ctacgcacac gtagactgtc cgggtcacgc cgactacgtt
      121 aaaaacatga ttaccggcgc cgcacaaatg gacggtgcaa tcctggtatg ttctgctgcc
      181 gacggcccta tgccgcaaac ccgcgaacac atcctgctgg cccgtcaagt aggcgtacct
      241 tacatcatcg tgttcatgaa caaatgcgac atggtcgacg atgccgagct gttggaactg
      301 gttgaaatgg aaatccgcga cctgctgtcc agctacgact tccccggcga cgactgcccg
      361 atcgtacaag gttccgcact gaaagccttg gaaggcgatg ccgcttacga agaaaaaatc
      421 ttcgaactgg ctaccgcatt ggacagctac atcccgactc ccgagcgtgc cgtggacaaa
      481 ccattcctgc tgcctatcga agacgtgttc tccatttccg gccgcggtac cgtagtcacc
      541 ggccgtgtag agcgaggtat catccacgtt ggtgacgaga ttgaaatcgt cggtctgaaa
      601 gaaacccaaa aaaccacctg taccggcgtt gaaatgttcc gcaaactgct ggacgaaggt
      661 caggcgggcg acaacgtagg cgtattgctg cgcggtacca aacgtgaaga cgtagaacgc
      721 ggtcaggtat tggccaaacc gggtactatc actcctcaca ccaagttcaa agcagaagtg
      781 tacgtattga gcaaagaaga gggcggccgc cataccccgt ttttcgccaa ctaccgtccc
      841 caattctact tccgtaccac tgacgtaacc ggcgcggtta ctttggaaaa aggtgtggaa
      901 atggtaatgc cgggtgagaa cgtaaccatt actgtagaac tgattgcgcc tatcgctatg
      961 gaagaaggtc tgcgctttgc gattcgcgaa ggcggccgta ccgtgggtgc cggcgtg
//
LOCUS       DAXFHF010000141         1003 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXFHF010000141 DAXFHF010000000
VERSION     DAXFHF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.150"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..217
                     /locus_tag="ACFIRM_002099"
     CDS             <1..217
                     /locus_tag="ACFIRM_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029623.1"
                     /translation="LNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYK
                     AYSETEPAGLDKRQLMKTLIQKCGFSC"
     gene            275..652
                     /locus_tag="ACFIRM_002100"
     CDS             275..652
                     /locus_tag="ACFIRM_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029624.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            697..>1003
                     /locus_tag="ACFIRM_002101"
     CDS             697..>1003
                     /locus_tag="ACFIRM_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxcD family protein"
                     /protein_id="HFC3029625.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     "
BASE COUNT          343 a          134 c          221 g          305 t
ORIGIN      
        1 tctgaatttc cgtatagtag aaaaagagca aaaagggttt tgggtaagta ccaaaatcaa
       61 aacagttgta tttgattcgg cagatatgcc ttctgatgat ctttttctga aatgcttaca
      121 gtcttcatat aaggcttatt ctgaaactga acctgccggt ttagataaac ggcagctgat
      181 gaaaacgtta attcaaaaat gcggattcag ttgttgacga aaaaatggat tctgaactaa
      241 cagaaactga tttcgaccat gtatgaggta aaaaatgtct ttcggctatt tgattgcaac
      301 atcgcaacct tgcgaattgt tgacaaaatc tcgaggtgaa actttttctt taattatgga
      361 taagatggat ttatggattt atttcagatt ttgtgaagga aatatttata caataaggaa
      421 gaatgaaact gaatcttgcc ttacagaaag aggaggtaaa tggttgaaac atatttatga
      481 atttaatcgg gaaagtttta ttttttctga tgtgcttcta caaaagggag aaagcgaaga
      541 gaatttttca gaaattgtct taaaatcaat aaaaaataat aaaattttga cagttgaggt
      601 acggtcaggc ttgcattttg acttaagaaa tatttatcgt attgagatgt aataatgcaa
      661 ttgatatgtg ccgattggac aggtatagga aattttatga aaaccttcga aaaaacatgg
      721 tctgcacaat atcgggatat ggaaatttca gtacggaatt tttggaattt gaaacggaca
      781 ggggcggaag tctatatcaa cggcaggcgg gtttatcata acgaagccgg gatggcgtct
      841 gcttctttgc gttcgctaat gggggaatac ctggaatttg aagaaagcgg tacgaaaatt
      901 accgttgaaa tcggcagcgc gtggcatttt aatgaaacta taagaaatat taatacacat
      961 ttaagaggta cgaaacttcc aggttctaac tggagagttc aag
//
LOCUS       DAXFHF010000142          993 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXFHF010000142 DAXFHF010000000
VERSION     DAXFHF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.151"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..462
                     /locus_tag="ACFIRM_002102"
     CDS             <1..462
                     /locus_tag="ACFIRM_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC3029626.1"
                     /translation="KDGKNPALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV
                     LSLTAAYRINGSKTLSDDVKYKAGNYWMLNPDISFAANDRISLTGGIQWLGKQPDRID
                     GKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTF"
     gene            494..>993
                     /locus_tag="ACFIRM_002103"
     CDS             494..>993
                     /locus_tag="ACFIRM_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029627.1"
                     /translation="MKKQITAAMMILAIIAALATAGSVDNQAFEDQVFHTRADAPMQL
                     AELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGD
                     VGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQG
                     IGRHRP"
BASE COUNT          271 a          253 c          240 g          229 t
ORIGIN      
        1 aaagacggca aaaatcccgc actcatcgct ttcctcgaaa gcacggttta cgaaaaatcg
       61 cgcaacaaag cctcgtcggg aaaatcgtgg ctcatcggcg ccaccaccta caaagccata
      121 gatccgattg tcctttccct caccgccgcc taccgcatca acggcagcaa aaccctttca
      181 gacgacgtca aatacaaagc aggcaattac tggatgctga atcccgacat ctcatttgcc
      241 gccaacgaca gaatcagcct gaccggaggc atccaatggc tgggcaaaca gcccgaccgc
      301 atagacggca aaaaagaatc ggcaagaaac acatccacct atgcccattt cggcgcaggt
      361 ttcggtttca ctaaaaccgc ggctttaaac gcatccgcac gtttcaacgt ttcagggcaa
      421 agcagttccg aactgaaatt gggcgtacag catacgtttt aagcaggctt tgatttttcc
      481 aacccttttt attatgaaga aacaaatcac cgcagccatg atgattctgg ctataatcgc
      541 cgccctcgca acagcaggca gcgtggacaa tcaggcattt gaagaccaag tgttccacac
      601 gcgggcagat gcgccgatgc agttggcgga actttctcag aaggagatga aggagactga
      661 aggggctttt cttccattgg ctatcttggg tggtgctgcc attggtatgt ggacacagca
      721 tggttttagt tatgcaacga caggcagacc agcttctgtt agagatgttg ctggcggatt
      781 aggcgcaatt cctggtgatg taggtgctgc aggaaaggtt gtttcctttg ctaaatatgg
      841 acgtgagatt aaaatcggca ataatatgcg gatagcccct ttcggtaata gaacaggtca
      901 tcctattgga aaatttcccc attatcatcg tcgagttacg gataatacgg gcaagacttt
      961 gcctggacag ggaattggtc gtcatcgccc ttg
//
LOCUS       DAXFHF010000143          982 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXFHF010000143 DAXFHF010000000
VERSION     DAXFHF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.152"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..518
                     /gene="mafB"
                     /locus_tag="ACFIRM_002104"
     CDS             <1..518
                     /gene="mafB"
                     /locus_tag="ACFIRM_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3029628.1"
                     /translation="TTVTRKTLDGQIAGKNKPIKPSSGDYVLPVGRQIIDPTRTSFSQ
                     ATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVDVVNMPDGAPTSMDNTRIMAAREAGV
                     KVEANVHNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKGVPEGWSKRFPN
                     GSIYDVKVLRK"
     gene            515..886
                     /locus_tag="ACFIRM_002105"
     CDS             515..886
                     /locus_tag="ACFIRM_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3029629.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          341 a          163 c          202 g          276 t
ORIGIN      
        1 aaacaactgt tacccgaaag actttagatg ggcaaatagc tggcaagaac aagcctatta
       61 aaccttcttc aggagattat gtcttgccgg taggcagaca aatcatagat cctactcgta
      121 catctttttc acaagctaca gttagctatc agaagcgtgg tgcaaattac aactatgatt
      181 ctcttgtagc tgcaatgaac gaaaaaaaat catgggttgg cgacagggtt gatgttgtta
      241 atatgcctga tggagcacct actagtatgg ataacacgcg tattatggca gcacgtgaag
      301 caggggtaaa agtggaagcg aatgttcata attttaatga ccgattatca tcaaaagaga
      361 gaatcaggtt taagcatgat ggtattgagc ctcaaacttg gggagaagct atccagctac
      421 gaattagaaa gcaagaaaca caaaaaggag ttccagaagg gtggagcaaa agatttccta
      481 acggaagtat ttatgatgta aaggtactta ggaaatgata aaacaaaata gttttgttcc
      541 gtatcctgaa gcaatgcttc ctaaaggatt taaatatccg caaagttatt taaaattagc
      601 tcaatccact catgccatta actacgatga acaatattct tttccttggt ggtttgaaaa
      661 tgcagaaagc aatatatcag aagtaattga catttatttt gaaataactg gcattccaaa
      721 cctattacct tttgctagaa accaagagtg ggctgcctgt tttgatattt cagataaatc
      781 aggtaatcct aaaattatag tagttaattt agataataca aaatattacg agacttttga
      841 aaattttgat acttggctaa aagaagctga aaatgatggt tggtagcagc cgtaggtcgg
      901 attctcgaat ccgacatttt ccaacagcgg catttcggaa acgatagacg cgtcaaatat
      961 ttttgtcgga tacaagtatc ca
//
LOCUS       DAXFHF010000144          977 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXFHF010000144 DAXFHF010000000
VERSION     DAXFHF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.154"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            111..317
                     /locus_tag="ACFIRM_002106"
     CDS             111..317
                     /locus_tag="ACFIRM_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC3029630.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            338..697
                     /locus_tag="ACFIRM_002107"
     CDS             338..697
                     /locus_tag="ACFIRM_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029631.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLDRI
                     TTNECINGIKKSIKKTN"
BASE COUNT          378 a          139 c          174 g          286 t
ORIGIN      
        1 ggaacataga cgagtgcgct atgatatcaa taacaatatc tatgttttgg ccaatccaaa
       61 aacattcaaa atcaaaacaa tgtttaaacc agacttagga aaggagtatt atgatggaga
      121 attcaaaaaa gacatgggaa attgacggag aaatatggct acattgtcct gtttgcggaa
      181 ctgaagttat ggactatgat atctgtgacg tttgtcagtg gcaaaataca ggagaaacta
      241 atatagatgg tggtcctaat gaaatgacac ttgcggaggc gaaagaagct tacgcaaaag
      301 gcttaccaat cagataaata agcacctaga gaaaccaatg atgacggaac cccatggtta
      361 cccatcaaat aatttacctg ttaaaatcat aaatgatatt atttatgcaa cccagttagt
      421 cgaagatttg gttttaggaa aaataaaaat tgttgatttt ttaaaatcat ataacaattt
      481 ttattattgg cttggttttg atgagttacc tcaatctgag aaaataaaat ttctaaacta
      541 tctcaatata ttaagtattc ataaggaaat acaagataaa actgtgaata gggtttatac
      601 cgattgtttt gatattgata aattacattc gcttgacaga attacaacta atgaatgtat
      661 taatggtata aaaaaatcta tcaaaaaaac aaattagaat ttgacaatat actcaaataa
      721 ctagcctatg ttttgtaaaa taaaaatatt cttgctgagt gtagagccag agataaaaag
      781 atttgatagt agtttgcagt tgtagaatta tataaacaag aaaatagtca gcagccgtag
      841 gtcggattct cgaatccgac attttccaac agcggcattt cggaaacgat agatgcgtca
      901 aatatttttg tcggatacaa atatccgacc tacatctctg cgcagcaaac tttacaagat
      961 attaatgaat taggaaa
//
LOCUS       DAXFHF010000145          946 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXFHF010000145 DAXFHF010000000
VERSION     DAXFHF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.156"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(1..233)
                     /locus_tag="ACFIRM_002108"
                     /pseudo
     CDS             complement(1..233)
                     /locus_tag="ACFIRM_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            complement(331..708)
                     /locus_tag="ACFIRM_002109"
     CDS             complement(331..708)
                     /locus_tag="ACFIRM_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC3029632.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            complement(739..>946)
                     /locus_tag="ACFIRM_002110"
     CDS             complement(739..>946)
                     /locus_tag="ACFIRM_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC3029633.1"
                     /translation="IGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFRVMLRYI
                     FTDLDVEDWKFFYFPIQHSKGRLR"
BASE COUNT          277 a          195 c          168 g          306 t
ORIGIN      
        1 tggatgcggt tggaaatcag ggtttggctg ttgccgatgc cgtctgaaac tttaatgtaa
       61 acggtttctt gatacggatt accgtatcgg gcggtaacgg gtttgtatcc tgttttcccg
      121 cttgcctcgt ccttggaaag gacgcggtct ccggcttgaa tatgggcgat ggctttgtag
      181 ccgtctgccg ttctgaccaa ggtgctgccg tggaaagagc aggtgtagga tttttgaaga
      241 acttagttcc caatatcctg tttaaaataa ggggaaggtc gcctgaaaat taaacacggt
      301 ttcagacggc attttgattg ccgggttttg ctattttttg ttgtaataat caaattgcac
      361 gttgactatg tctttctcgg taaaaatata acggagcatt gttttaagcc tttcataacg
      421 ttcgttaatt cccacgctat caggtaacca aggggaagct ttaatttcaa aaagtttcca
      481 atttggaacc attaaaaact caataatggt accgattcca atcacgatat cccttggtat
      541 atccatcgga taaggatatt ttctcctaac ctcgattaaa tcattctcca acttccaata
      601 ttcttcatca tcccacaccc cgtcatcata ccatttgcca ataaatgaat tttcgtcata
      661 cccctcaaaa taaggaacgt ttcttataat atccttgaac tcacacataa taatgtatct
      721 ccaatataat taaacttttc atctcaatct acctttacta tgttgtattg gaaagtaaaa
      781 aaatttccag tcctctacat ctagatcagt aaaaatataa cggagcatta ctctgaacct
      841 ttcataacgc tcattaattt tgacactttt aggcaaccaa ggagaagctt taatttcaaa
      901 aagtttccaa ttttgaacca ttaaaaaatc aataatggta ccgatt
//
LOCUS       DAXFHF010000146          883 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXFHF010000146 DAXFHF010000000
VERSION     DAXFHF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.157"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>883
                     /locus_tag="ACFIRM_002111"
     CDS             <1..>883
                     /locus_tag="ACFIRM_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3029634.1"
                     /translation="LNRCTFDWNGGGCAVNKGDDFRAGASFSLGRNPKYKEEMDAKKP
                     EEILSLKVDADPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVAATFGRD
                     AQGNTTADVQVIPRPDLTPASAEAPHAQPPPEVSPAENPANNPDPDENPGTRPNPEPD
                     PDLNPDANPDTDGQPGTSPDSPAVPDRPNGRHRKERKEGEDGGLSCDYFPEILACQEM
                     GKPSDRMFHDISIPQVTDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYRASYEPLCVFA
                     EKIRFAVLLAFIIMSAFV"
BASE COUNT          239 a          250 c          225 g          169 t
ORIGIN      
        1 ttaaatcgtt gcacatttga ttggaacggc ggaggttgtg cggtcaataa aggtgatgat
       61 ttcagagctg gggcttcttt ttcccttggc cgcaatccga aatacaaaga agaaatggat
      121 gccaaaaagc cggaagagat tttatcgttg aaagtcgatg ccgatcccga caaatacata
      181 aaggcaaccg gatatcccgg ttattccgaa aaagtagaag tcgcacccgg aacaaaagtg
      241 aatatggggc ccgtcacgga caggaacggg aatcccgttc aggttgccgc aacattcggc
      301 agggacgcgc aaggcaacac cacggcggac gtacaagtaa tcccgcgtcc cgacctcacg
      361 cccgcaagcg cggaagcacc tcacgcacag ccgccgcccg aagtatcgcc cgcggaaaac
      421 cccgcaaaca acccggaccc cgatgagaac cccggcacgc gtcccaatcc cgaacccgac
      481 cccgatttga atcccgatgc aaatcccgat acggacggac agcccggaac aagccccgat
      541 tccccggccg ttccggaccg cccaaacggc aggcatcgca aagaaaggaa agaaggcgaa
      601 gacggcgggc tttcgtgcga ttattttccg gaaatcctag cctgtcagga gatgggcaaa
      661 ccttcggacc gcatgtttca cgatataagc ataccgcagg ttacagacga taaaacatgg
      721 tcttcacata actttttacc gtctaacggc gtatgtccgc agccgaaaac ctttcatgtt
      781 ttcggcaggc aatatcgggc aagctatgaa ccgttgtgcg tgtttgccga aaaaatccgg
      841 tttgccgtac tgctcgcctt tatcattatg tcggcttttg tcg
//
LOCUS       DAXFHF010000147          871 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXFHF010000147 DAXFHF010000000
VERSION     DAXFHF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.158"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..328
                     /locus_tag="ACFIRM_002112"
     CDS             <1..328
                     /locus_tag="ACFIRM_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFC3029635.1"
                     /translation="CPYGGSNNLEKAKLRAERLSKNDRAGKDFTKAGKEAVIDLNRIQ
                     NNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVIPKSKGGQGTPKNGQVLCRGCN
                     IKKSNK"
     gene            325..744
                     /locus_tag="ACFIRM_002113"
     CDS             325..744
                     /locus_tag="ACFIRM_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC3029636.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          313 a          139 c          172 g          247 t
ORIGIN      
        1 ttgtccgtat ggcggttcaa ataatttaga gaaagcaaaa cttcgcgcag aacggcttag
       61 taaaaacgat cgggcaggaa aagactttac taaagcagga aaagaagccg ttattgattt
      121 gaataggatt caaaataatg gtcaagttaa gtgcgctaat tgcggtattg aaacaattcc
      181 agccaaacaa tctataaaaa atatttctcc tacttcaaat gaaagacaag tagatcatgt
      241 catccctaaa tctaagggtg gtcaaggtac acctaaaaac gggcaggtat tatgcagagg
      301 ctgcaacatt aagaagagta acaaatgaaa tcgaaactta ctgtcgtcta ttacgatttg
      361 gaaagcaata ttgcagaaga aatactgtca ggaaacataa tgcctgatgg aaattttctt
      421 attcaagaga ttcctctttt cgcaccgaat ttggctttaa acgacattgt tgccatagaa
      481 cgtgaggata agatgctgtt tttcgaccac ttgataaaag cttcaggaaa taccacgata
      541 aacatcgttg ttttggatca tttcccaaag gatttattgg cagccataga agaacacagt
      601 ggtaaaatca gaaaaaatgg agagaattat ttatcggtta attttccgcc caaaaaatat
      661 aattctgatt taaaaggaat tttaaataga tatgaggaag caaatatcct cagctacagg
      721 gaagcttgtt tgggcttctc ttaatcgatc tataagtaag aatggattac gttagattgt
      781 aaacctaacg attaaaaggt cgtctgaaac cgtgtttaat tttcagacga cctatttcct
      841 tcatttgaaa caggatattg ggaattaagt t
//
LOCUS       DAXFHF010000148          830 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXFHF010000148 DAXFHF010000000
VERSION     DAXFHF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.160"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>830
                     /locus_tag="ACFIRM_002114"
     CDS             <1..>830
                     /locus_tag="ACFIRM_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029637.1"
                     /translation="NKVTFENVTIAAHNAPAGILSDDRHKSSSLASAMLAFKGRNTIN
                     MDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDKGQGITANHYGNS
                     DINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSSRKLGNKKQIPEGSKME
                     AIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKVVKIKGDIFAYNG
                     GSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPDTKSIEKKKKELN
                     "
BASE COUNT          273 a          169 c          212 g          176 t
ORIGIN      
        1 aaaataaggt tacttttgaa aacgtaacca tagccgccca taacgcgcct gccggcattt
       61 tgtctgatga taggcataaa tccagtagct tggcgtcggc gatgcttgcc tttaaagggc
      121 gcaataccat caatatggac gcggactcaa atgccaactc tagtaacgaa ggtatcctat
      181 tgcttaataa tggtgaaaaa atgggagaat atcgtcttgt ttccgaagaa ggctcgacgc
      241 tgaatatcaa catcaaatca ggaaaagata aaggacaggg cattaccgcc aaccactatg
      301 gtaactcaga catcaacttc aacaaggctt ctccaaacat caccacaatg gagttcaagg
      361 gcgatgtaaa cattaaaatc gataggaacg gacaggaaga ggcggaaagc aacggttttg
      421 gcttctattc aagccgcaaa ttaggcaata agaagcagat tccggaaggt tctaagatgg
      481 aagcgatttt ccgtgggaac gtcgatatcg ttgcaacgcc ggtttatgac gagcaaggca
      541 ggcccaaaag cataggcagc gcgtttgcga ttgacgggaa atacagcaaa gtcgaggttg
      601 tcggcgggga aggcaaggtt gtcaaaatca aaggcgatat tttcgcctac aacggcggca
      661 gcgtgagcgt gaaccttgcc aacaaagact cttattttga aggggaagcc catatcggga
      721 aaaggagctt tgccaaaggg aaagatatgt ttgccttaac cgtggatgcg gacggatatg
      781 aattaacccc cgatacaaag tcgattgaaa agaaaaagaa agagctgaat
//
LOCUS       DAXFHF010000149          795 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXFHF010000149 DAXFHF010000000
VERSION     DAXFHF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.164"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(202..486)
                     /locus_tag="ACFIRM_002115"
     CDS             complement(202..486)
                     /locus_tag="ACFIRM_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3029638.1"
                     /translation="MICTVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
     gene            complement(590..>795)
                     /locus_tag="ACFIRM_002116"
     CDS             complement(590..>795)
                     /locus_tag="ACFIRM_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029639.1"
                     /translation="KCGIDGIGGLKPSLQSFQTAFFAFFGRGRQMKRVIPAQAGIRVC
                     RCGNLSGKKVSQVLSPGFPLSRE"
BASE COUNT          220 a          219 c          148 g          208 t
ORIGIN      
        1 cagaaaccta aaatcccgtc attcccacga aagtgggaat ctaggtctgt cggcacagaa
       61 acttatcggg taaaacggtt tctttagatt ttacgttcta gattcccgcc tgtgcgggaa
      121 tgacggttta gaagttgccc gaaacctcaa aaaaaaccga aaccgaacaa gccggattcc
      181 cgcctgcgcg ggaatgacgg gctaaataat atcaaaccat aaatcccgcc aagaaacatt
      241 attttcttca atcagttgca atttccaagc cctgttccat ttcttcaact gtttttcccg
      301 agtaattgcg ctctccatcg taggatgcag ttcataccaa accggcatag taacgttgta
      361 ccgtgatgta aatccttcaa tcaaatgctc cctatgttgg taaatacgtt gcaccaaatc
      421 agatgtaacg ccaatgtata acgtgccatt acgttggctt gctaaaatat aaaccgtgca
      481 tataataccc ttttgaatta tttaaattta tattcccgcg aacaccatcc ggtgattact
      541 ttaacccttc gttatcccca tagctttcca tcattcccgc aactcttcgt cattcccgcg
      601 aaagcgggaa tccaggactc aggacctgag aaaccttttt acccgataag tttccgcacc
      661 gacagacccg gattcccgcc tgcgcgggaa tgacgcgttt catttgccgc cccctcccga
      721 aaaacgcaaa aaatgccgtc tgaaagctct gtagggaggg cttcagcccg ccgattccat
      781 caatcccgca tttcc
//
LOCUS       DAXFHF010000150          778 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXFHF010000150 DAXFHF010000000
VERSION     DAXFHF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.166"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..108)
                     /locus_tag="ACFIRM_002117"
     CDS             complement(<1..108)
                     /locus_tag="ACFIRM_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC3029640.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGL"
     gene            188..417
                     /locus_tag="ACFIRM_002118"
                     /pseudo
     CDS             188..417
                     /locus_tag="ACFIRM_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(454..>778)
                     /locus_tag="ACFIRM_002119"
     CDS             complement(454..>778)
                     /locus_tag="ACFIRM_002119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029641.1"
                     /translation="HGKSPKCLGGNLGDFRGILQRSSGLYYKIWEGGWRMPALPFFRT
                     SIPMLVQNRMESIFNHNKDAIFLEGWSLGDAAMIMELLWQNISTSRRELTKLFLYKNF
                     WGCTR"
BASE COUNT          235 a          161 c          149 g          233 t
ORIGIN      
        1 taaacctttt gaatcgttgt caaactttgc cgaatattcg gcattgccga caatggccgt
       61 tgcgtcaaaa gtcaacttgg atatgtccaa tcctacggtg ttacgcatgg gattaccctt
      121 atatattgcg tccctaagaa ggggcgatta acaaaaatta atgccctttc ttagggacgc
      181 aatatataag gtttataccg tcgttgtccc taatgcgcaa tcagcgacat tgttgccaat
      241 tatccgaaag aaagttaagc ctgatggcat tgtatacacg gatacctttc gtagttatga
      301 tgtgcttgat gttagtgaat ttagccattt acgtaagttt aacggcattc ccaaagagca
      361 tttggggcta tatttaaaga aatgccaatg gcatttgaaa tagaatctca aatttagatt
      421 ttaagacaat tagttaatgg gaatttggtc tggttatcta gtacaacccc aaaagttttt
      481 atataaaaac agtttggtaa gttctcttcg tgaagtgctt atgttttgcc acaaaagttc
      541 cataatcatg gcggcatcac ccaagctcca tccttccaag aaaatggcat ctttgttgtg
      601 gttgaaaatc gactccatac gattttggac aagcataggg atagatgttc tgaaaaacgg
      661 cagcgcgggc attctccaac caccttccca aatcttataa tacaaacccg aagacctttg
      721 caaaattccc ctaaaatccc ctaaattccc accaaggcat ttaggggatt tcccatga
//
LOCUS       DAXFHF010000151          731 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXFHF010000151 DAXFHF010000000
VERSION     DAXFHF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.171"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(113..304)
                     /locus_tag="ACFIRM_002120"
     CDS             complement(113..304)
                     /locus_tag="ACFIRM_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029642.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(419..>731)
                     /locus_tag="ACFIRM_002121"
     CDS             complement(419..>731)
                     /locus_tag="ACFIRM_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029643.1"
                     /translation="FKNIERYGYQSSDIKKINPPFPLNILPVPKPYYFYLYDTHLNQI
                     YDLAWSCNDFLIFEIFILDRESIKYIDRDKLFKPGETLYLNFKSIKNQCKNYFFLRFW
                     F"
BASE COUNT          279 a          142 c           95 g          215 t
ORIGIN      
        1 aagataaaga aacggcaaca cacgccaaca gaaaaacata tttgaacttc atcatatttt
       61 ccacataaaa ggcagcctga aaatctttca ggctgccctt gtcaaattat tcctagcttt
      121 cggctttttt ggcaaaccaa acaatccgat tacccgcata atactttcca tttattgaaa
      181 tccgacaagc cgcgcccaaa aaatgccatg cactgccgat ttccgcagca atctttgtac
      241 cgttttcttc aaattccaaa tattcaccca ataataaact tgaaacagaa cacgcgggca
      301 gcaagtgccc accctacgct tattcaaata atttgattaa ataaagaata aagaaaaggc
      361 atcaggaaca aaattataat ctgccacttg actcgcaccg cttttaaagt aaggggcatc
      421 aaaaccaaaa ccgcaaaaaa aaataatttt tgcattgatt tttaatagat ttaaaattca
      481 aatatagtgt ttctccaggt ttaaaaagtt tatctctatc tatatatttg atgctttccc
      541 tatccaaaat aaatatttca aatattaaaa aatcattaca tgaccaagcc aaatcataaa
      601 tttgattcaa atgggtatca taaagataaa aataataagg tttgggaaca ggtaaaatat
      661 ttaatggaaa aggaggatta attttcttaa tatctgatga ctgatatcca tatctttcta
      721 tattcttaaa a
//
LOCUS       DAXFHF010000152          721 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXFHF010000152 DAXFHF010000000
VERSION     DAXFHF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.172"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..401
                     /locus_tag="ACFIRM_002122"
     CDS             <1..401
                     /locus_tag="ACFIRM_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3029644.1"
                     /translation="WPANNGAAGVASASDIKGKYVKSVTVAKGVVTAQMASSNVNKEI
                     KGKKLSLWARREDGSVKWFCGQPVTRDDNGNVTADDTDNINTKHLPSTCRDAASAVCI
                     ETPRADFKHFQKISRCRVLPESRHMARKQR"
     gene            340..711
                     /locus_tag="ACFIRM_002123"
                     /pseudo
     CDS             340..711
                     /locus_tag="ACFIRM_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          217 a          221 c          173 g          110 t
ORIGIN      
        1 aatggcccgc aaacaacggc gctgccggcg tggcatccgc ctccgacatc aaaggcaaat
       61 atgttaaaag cgttacggtc gcaaaaggcg tcgttaccgc ccaaatggct tcaagcaacg
      121 taaacaaaga aatcaaaggc aaaaaactct ccctgtgggc caggcgtgaa gacggttcgg
      181 taaaatggtt ctgcggacag ccggttacgc gcgacgacaa cggcaacgtt accgccgacg
      241 acaccgacaa catcaacacc aagcacctgc cgtcaacctg ccgcgacgca gcatctgccg
      301 tttgcataga aacaccacgc gccgatttca aacacttcca aaaaatcagc cgttgccggg
      361 tattacctga atcacggcat atggcccgca aacaacggtg atgccggcgt ggcatccccc
      421 ccctccaaca tcaaaggcaa atatgttaaa gaggttgaag ttaaaaacgg cgtcgttacc
      481 gccacaatgc tttcaagcgg cgtaaacaaa gaaatccaag gcaaaaaact ctccctgtgg
      541 gccaggcgtg aaaacggttc ggtaaaatgg ttctgcggac agccggttaa gcgcaccgcc
      601 gacgccgcca aagccgacaa cgacgaagtc gccaaagacg ccgccgccga caacggcatc
      661 aacaccaagc acctgccgtc aacctgccgc gatgaatcaa ctgccaccta aggcaaatta
      721 g
//
LOCUS       DAXFHF010000153          714 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXFHF010000153 DAXFHF010000000
VERSION     DAXFHF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.173"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..537
                     /locus_tag="ACFIRM_002124"
     CDS             <1..537
                     /locus_tag="ACFIRM_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC3029645.1"
                     /translation="QWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWR
                     GRDFTPEERDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDN
                     AVFAFDLSDPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRTEH
                     PQGNAAPAEDIEDDIPFN"
BASE COUNT          210 a          220 c          189 g           95 t
ORIGIN      
        1 cagtgggaaa tcgacccgga aggcgatccg gaaatgctga tgccggacgg caggccctac
       61 ctcatcagcc gccgatacac cgccagcctg cacagcaaaa gccaactggc aacagacctc
      121 aaaagctggc gcggaaggga ctttacaccg gaagaacgcg acaacttcga tttgcgcaat
      181 attttgggca agccctgcct gttgagtatc gcccaccaag aaagcagcga cggcaaaacc
      241 acctatgcca acatttccgc catcagcaac aagatgaaga gctacacccc gaaacagccg
      301 gacaacgccg tttttgcctt cgacctgtca gaccccgatt gggcgaatta cggcctcctg
      361 aacgagaaac tgagggagca gattgccaaa agcccggaat atgccgaagc cgtaaacggc
      421 cgccaaccgc cggcgccgcc gcagaaacag gcccaagcgg cggaagggcg gacggaacac
      481 ccccaaggca atgccgcgcc tgccgaagac atcgaggacg acatcccttt taattaagcc
      541 cgccgtcagg gcgtaaaacc ggacggaaaa ccgcaaacaa acgccgcgcc ctacgggcgg
      601 gctccgcact ctgaagcagt agtcgatttt tagtttgcaa ctgcttcgga cggcaacaga
      661 gaaaggaaac aaaaaaatgg caaacatcga cctgacccaa tgggacggga aaac
//
LOCUS       DAXFHF010000154          710 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXFHF010000154 DAXFHF010000000
VERSION     DAXFHF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.174"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..256
                     /locus_tag="ACFIRM_002125"
     CDS             <1..256
                     /locus_tag="ACFIRM_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HFC3029646.1"
                     /translation="FDESNGADYFYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHI
                     LKLINDILEICNSTSEDKSISNLAKYFDWEFDDYNLNIQD"
     gene            333..542
                     /locus_tag="ACFIRM_002126"
     CDS             333..542
                     /locus_tag="ACFIRM_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029647.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
BASE COUNT          259 a           86 c          140 g          225 t
ORIGIN      
        1 ttttgatgaa agtaatggtg ctgattattt ttatgtttat atatatagca ccgaatggtt
       61 actttcaaat atacatagac cgatgtcatt aaaaaactca attgttacaa accgttttaa
      121 tattgaacac atattaaaat tgataaatga tatattggaa atatgtaatt caacgtctga
      181 agataagtct atatcaaatt tggcaaaata ttttgactgg gaatttgacg attataatct
      241 aaatatccaa gactaaagat ggtgggacag catttagacc aaaaacaggt aaacagtatt
      301 ttgatgacaa attaaaataa aaggaattat tcatgaaatt aattgatttt gaagggaatt
      361 tagttaaaat tagtctcgat aaagatgagt tgtatattat tcaagcaatt gtaggagaaa
      421 tttattcagg cgtatgcgtg gattgtagag attttgagat aattcatggt gttgaaaaaa
      481 ataaagttct tcttttggat aaagaactta aaaaaatata tgatacttgg gataaatgtt
      541 agctagatag ggtgggcact cgttgcccac cttttgccgt atattaaaaa tccttttcag
      601 gctgcctgaa actgggtggg catcaatgcc aatcctaact gtgcggatga agcaggaaaa
      661 ctgatatggg aagacgaccc cgataaaaat tggcgggcta accgtatgga
//
LOCUS       DAXFHF010000155          696 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXFHF010000155 DAXFHF010000000
VERSION     DAXFHF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.175"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(84..386)
                     /gene="imm40"
                     /locus_tag="ACFIRM_002127"
     CDS             complement(84..386)
                     /gene="imm40"
                     /locus_tag="ACFIRM_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC3029648.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQIYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(402..>696)
                     /locus_tag="ACFIRM_002128"
     CDS             complement(402..>696)
                     /locus_tag="ACFIRM_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029649.1"
                     /translation="ESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFSENG
                     TVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
BASE COUNT          216 a          125 c           83 g          272 t
ORIGIN      
        1 gccggtcagt gtaggttgga ttaaaaccca gcattttcaa catcttgatg ttttactata
       61 aacctattct tgtgcttcct ttgttagata aatttcaaaa caaaagacac gtatgcattc
      121 tctaatttta tttgagataa ataatgcatt gctttgttga tactgttaaa taaattactt
      181 ccctcgtaat accaattatc atatatttga taaaaataat tatctttctt gatataaata
      241 tctcccccca atacaagtat tctttcattt ataaataaat ttaatatatc tattgcagtg
      301 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
      361 taaatttcaa aaatttcatt taacatatta atctctatct actatttttc aggaagttgt
      421 tggttaggga caaataatct atgattaatt tttccatctg cttctctgat atattcaaaa
      481 tttccatctt ttcctttata agatccaggg atataaagtt tatgccttac aatattatct
      541 ccgccaacta tttgtattac tgttccattt tcagaaaaag catctactga ttgcggaaaa
      601 ccatgataat caccattcga tgcttgcctt aatactttct ctgtataacc ggtattttca
      661 aaaaaattat tcgttgttac agtatttttt gattcc
//
LOCUS       DAXFHF010000156          656 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXFHF010000156 DAXFHF010000000
VERSION     DAXFHF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.180"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            460..>656
                     /gene="pilE"
                     /locus_tag="ACFIRM_002129"
     CDS             460..>656
                     /gene="pilE"
                     /locus_tag="ACFIRM_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HFC3029650.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGTWP"
BASE COUNT          223 a          183 c          106 g          144 t
ORIGIN      
        1 cacggaaacc taccgagatg cgtcaactgc cgtttgcacg aaacaccacg cgccgatttc
       61 aaatgctttc caagaaaacg gagcttttta aaaaataaaa aattccccac ccaacccacc
      121 ctattctaac gcgtaaattc aaaaatctca aattccgacc caatcaacac acccgatacc
      181 ccatgccaat aaaaaagtaa cgaaaatcgg cactaaaact gacaattttc gacactgccg
      241 ccccctactt ccgcaaacca cacccaccta aaagaaaata caaaataaaa acaattatat
      301 agagataaac gcataaaatt tcacctcaaa acataaaatc ggcacgaatc ttgctttata
      361 atacgcagtt gtcgcaacaa aaaaccgatg gttaaataca ttgcatgatg ccgatggcgt
      421 aagcctgagg catttcccct ttcaattagg agtaatttta tgaataccct tcaaaaaggc
      481 tttaccctta tcgagctgat gattgtgatc gccatcgtcg gcattttggc ggcagtcgcc
      541 cttccggcat accaagacta caccgcccgc gcgcaagttt ccgaagccat ccttttggcc
      601 gaaggtcaaa aatcagccgt taccgagtat tacctgaatc acggcacatg gccgga
//
LOCUS       DAXFHF010000157          633 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXFHF010000157 DAXFHF010000000
VERSION     DAXFHF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.182"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(217..360)
                     /locus_tag="ACFIRM_002130"
     CDS             complement(217..360)
                     /locus_tag="ACFIRM_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029651.1"
                     /translation="MSDKQARFRGGGFDGEKECAAGNEVSAITRHRILFVVGVLEKEW
                     FVV"
     gene            383..571
                     /locus_tag="ACFIRM_002131"
     CDS             383..571
                     /locus_tag="ACFIRM_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029652.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTKNLGAVLDYP"
BASE COUNT          167 a          177 c          128 g          161 t
ORIGIN      
        1 gtctcaagaa gattatcaag ttccttcttg gatagaatat ataaaagggt ggattagaga
       61 accatagccc acattcccaa ccacgtgtgt ggtgtaacta cgcacgtggc tgcttgattg
      121 acggtttgcc gtttcaactt ttaacttcgt tgaagctagt gctttcagac ggcctccaat
      181 gtcgtttgga aacatcaggt ccaacaaagt taaaccttaa acaacaaacc attccttctc
      241 caacacccca accacaaaca aaatcctatg gcgcgttatc gcagaaactt cattgccggc
      301 ggcacattct ttttcaccgt caaacccgcc gccccgaaat cgcgcctgct tgtcggacat
      361 atcggcttcg cgggctgcct atatggatgt gcaaaaacaa tatccctttg aaaccgtcgc
      421 cgtatgcgtg ctgccgaacc atattcacgc catttggacg ctgccgcccg acgatgcgga
      481 ttattcccta ctccggcggc tgattaaaac caaattctcc gcatattccc ctcatactaa
      541 aaacttaggg gctgtactag attatcccta aattccacac cgatcccgca ggatttttag
      601 ctgccgggac ggtgtgccga agttaagtcg aaa
//
LOCUS       DAXFHF010000158          628 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXFHF010000158 DAXFHF010000000
VERSION     DAXFHF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.183"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>628
                     /locus_tag="ACFIRM_002132"
     CDS             <1..>628
                     /locus_tag="ACFIRM_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3029653.1"
                     /translation="ITDFIQSILSSITSDTPYSWIFCEGSSEKIYFDKYFEDEIQNFN
                     LRIIPTGGAKKIKRIYQNILTPYGEIKENIDGNTYNKWGKILFLSDTDNQLTHYEAKN
                     YNNQYSRLQCYRLINNKSTRLTETVAINDSLTSPATEIEDCLNGKLLVETLKYFREIK
                     EYEYLESIIDKDKEYSEEASYFSLNLRPSEADKLKEFFDSEDGDMKIKF"
BASE COUNT          255 a           78 c          102 g          193 t
ORIGIN      
        1 attacagact ttattcagtc tattctctct agcattacat ctgatactcc atacagttgg
       61 attttctgtg agggtagttc ggaaaaaatt tattttgata aatattttga agacgagatt
      121 caaaatttta atttgcgaat tattccaaca ggaggtgcaa agaaaattaa acggatatat
      181 caaaatattt tgaccccgta tggagaaatt aaagaaaata ttgatgggaa cacatacaac
      241 aaatggggaa aaatactgtt tttgtcagat acagataatc aacttactca ttatgaagca
      301 aaaaactaca ataaccaata ttccagattg caatgctata gattgattaa taataaatca
      361 acaagattga ctgaaacagt agcaataaat gactcattaa cctctccggc aactgaaatt
      421 gaagattgct taaatggaaa attacttgtg gaaacattga aatattttag agaaataaaa
      481 gagtatgaat atttagaaag tattatagat aaggataaag aatattctga ggaagcttca
      541 tatttttcac tcaatttacg accaagtgaa gctgataaat taaaagaatt ttttgattca
      601 gaagatggag atatgaaaat taaattcg
//
LOCUS       DAXFHF010000159          615 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXFHF010000159 DAXFHF010000000
VERSION     DAXFHF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.185"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..140)
                     /locus_tag="ACFIRM_002133"
     CDS             complement(<1..140)
                     /locus_tag="ACFIRM_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFC3029654.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VV"
     gene            complement(142..420)
                     /gene="secE"
                     /locus_tag="ACFIRM_002134"
     CDS             complement(142..420)
                     /gene="secE"
                     /locus_tag="ACFIRM_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC3029655.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(535..610)
                     /locus_tag="ACFIRM_002135"
     tRNA            complement(535..610)
                     /locus_tag="ACFIRM_002135"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Trp,seq:cca)
BASE COUNT          197 a          155 c           86 g          177 t
ORIGIN      
        1 tcaacaactt tctctacagg caccagaatt tgtccgaaat aatctcccat ctcctcacgg
       61 gcaattcgct cttccaatat tcgttggaca ttcttctcaa accccgaata cgcctgtaca
      121 acataccatt ttttcgacat ctcaaccttc ccttctcagc aatacatcaa aaaataacca
      181 cgaaattgct gtatctgccg catagataaa tatagaaagc acagcaacaa acactataac
      241 aaatacagtc attctgacgg catcttcacg cttaggccaa accacctttt tgaattcgga
      301 ccaagaattt gagaaatatg caaaaaaacc ctccttaccg gaattagatg cagattcttt
      361 atcttgaaca accagttgat tcactttaac attctttttt tcaggcgtat gttctgtcat
      421 attatttatc catcatagca tctgtctatt ctcaatccat gtaaatggca agagagttta
      481 ctaaataaca aatacaaaaa aattaaccgg cacaaggccg gttaattttt tatttggcag
      541 gccaagaggg tctcgaaccc ccaaccctcg gttttggaga ccgatactct accaattgag
      601 ctattggcct ctaaa
//
LOCUS       DAXFHF010000160          595 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXFHF010000160 DAXFHF010000000
VERSION     DAXFHF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.189"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..519
                     /locus_tag="ACFIRM_002136"
     CDS             <1..519
                     /locus_tag="ACFIRM_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3029656.1"
                     /translation="FGGWRIAADYARYRKWNNNKYSVNTKNVRTHPDNGTREDLKTEN
                     QENGSFHAASSLGLSAIYDFQINDKFKPYIGARVAYGHVKHQVHSVETGTTIVTSKNG
                     GPIPEAPTRKPAYHESRSISSLGFGAVAGVGIDITPNLTLDAGYRYHNWGRLENTRFK
                     THEASLGMRYRF"
BASE COUNT          164 a          174 c          144 g          113 t
ORIGIN      
        1 ttcggcggct ggaggatagc ggcagattat gcccgttaca gaaaatggaa caacaataaa
       61 tattccgtca acacaaaaaa tgtgcggaca catccagaca atggcaccag ggaagacctg
      121 aagacggaaa atcaggaaaa cggcagcttc cacgccgctt cttctctcgg cttatccgct
      181 atttacgatt tccaaataaa cgataaattc aaaccctata tcggcgcgcg cgtcgcctac
      241 ggacacgtta aacatcaggt tcattcagtg gaaacaggaa ccacgattgt tacctctaaa
      301 aatggaggtc ctatcccaga agctccgacc agaaaacctg cctatcacga aagccgcagc
      361 atcagcagct tgggcttcgg ggcagtggca ggcgtaggca tcgacatcac gcccaacctg
      421 accctggacg ccgggtaccg ctaccacaac tggggacgct tggaaaacac ccgcttcaaa
      481 acccacgaag cctcattggg catgcgctac cgcttctgat tccccgacac cgatgccgtc
      541 tgaaccttca gacggcattt ttgatgcgct cgccgtttac aggcgcgggg cgggc
//
LOCUS       DAXFHF010000161          591 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXFHF010000161 DAXFHF010000000
VERSION     DAXFHF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.191"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..579
                     /locus_tag="ACFIRM_002137"
     CDS             <1..579
                     /locus_tag="ACFIRM_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3029657.1"
                     /translation="LAFGYDFGGWRIVADYARYRKWHNNKYSVNIKELQGRNNITSGG
                     SQLNIKYQKTEHQENGTFHAVSSLGLSTVYDFRPNDKFKPYIGVRVGYGHVRHSIDST
                     KKTRNTLTAYHGDGRGSTYYDDIEPGENQKNTYRQNRSSRRLGFGAMAGVGIDVAPGL
                     TLDAGYRYHYWGRLENTRFKTHEASLGVRYRF"
BASE COUNT          163 a          165 c          142 g          121 t
ORIGIN      
        1 cttgccttcg gctacgattt cggcggctgg cgcatcgtcg cggattatgc ccgttacagg
       61 aaatggcaca acaataaata ttccgtgaac ataaaagagt tgcaaggaag gaataatatc
      121 acttctggcg gcagccagct taacataaaa taccaaaaga cggaacatca ggaaaacggc
      181 acattccacg ccgtttcttc tctcggcttg tcaaccgttt acgatttcag acccaacgat
      241 aaattcaaac cctatatcgg tgtgcgcgtc ggctacggac acgtcagaca cagtatcgat
      301 tcgactaaaa aaacgagaaa tactcttacc gcctaccatg gtgatggcag gggatctacg
      361 tattatgatg atatagagcc gggagaaaac caaaaaaaca cttatcgcca aaaccgcagc
      421 agccgccgct tgggcttcgg cgcgatggcg ggcgtgggca tagacgtcgc gcccggtctg
      481 accttggacg ccggctaccg ctaccactat tggggacgcc tggaaaacac ccgcttcaaa
      541 acccacgaag cctcattggg cgtgcgctac cgcttctgat cccccgattc c
//
LOCUS       DAXFHF010000162          584 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXFHF010000162 DAXFHF010000000
VERSION     DAXFHF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.194"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..245)
                     /locus_tag="ACFIRM_002138"
     CDS             complement(<1..245)
                     /locus_tag="ACFIRM_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC3029658.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEE"
     gene            complement(373..>584)
                     /locus_tag="ACFIRM_002139"
     CDS             complement(373..>584)
                     /locus_tag="ACFIRM_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029659.1"
                     /translation="VPRIKENGQIIRPEYNGSMWKMSDGQPLRLSLAECSPKDNLQSG
                     LETGRIVFGVLASVYFVSLLKKVLK"
BASE COUNT          170 a          137 c          102 g          175 t
ORIGIN      
        1 ttctcttctg attttcttat aaaaataaca gactcatcag aaaaataaat cttccaaata
       61 ttatgtgcga ccctcttatt taagaaataa gagaagctct ctaaaatatc gtactctttt
      121 acgttattaa caaattcttc aaattctttc tgagcaataa aagccatcga ttgcccaaaa
      181 tactcgcttg acggctgata tttataaagt gccaactgcg cctgagtgat aaacggcttg
      241 ttcatggttc tgcctttcaa aggttgtttt gaaagcctga ttttgacacc ataacttcat
      301 gcgctcaatc cttaaacaga accgccccga ttaatacggg gacggcaacg ccgagataga
      361 aataaaaatc catcatttca aaaccttttt cagcagggaa acaaagtaaa cggacgcgag
      421 gacgccgaat actatccggc ctgtttcaag accgctttgc aggttgtctt tcggactgca
      481 ttccgccaat gaaagcctta gcggctgacc gtccgacatc ttccacatgc tgccgttata
      541 ttccggcctg attatctgtc cgttttcttt gattcttggt acta
//
LOCUS       DAXFHF010000163          579 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXFHF010000163 DAXFHF010000000
VERSION     DAXFHF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.196"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            255..>579
                     /locus_tag="ACFIRM_002140"
     CDS             255..>579
                     /locus_tag="ACFIRM_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029660.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVT"
BASE COUNT          170 a          129 c          162 g          118 t
ORIGIN      
        1 caaaaataaa atagggaggg atgtcgtgat atttgaaaaa tcaataaacg gctggaaata
       61 ccgcgccgta atggaaatca gaaccacgca aaaggagctt gccttgaaca gcctttatac
      121 aacagacagg attgaatagt catgacaaaa cctgcgacga gtaactgtca atctttcgat
      181 tccgtcctca tacatcctaa gcgtctttgc aaattctgtt cagttatacc atttatacag
      241 acaggagcaa accaatgacg aagttatacg cagaaatcgc cgaaatggag gcgcaggacg
      301 acggcacggt caaagtttgg ggttatgcct caagcgaggc ggtcgattcg gacggcgaag
      361 tcatcgcggc ggcggctatg aaggcggcga ttcccgatta tatgaagttc ggcgaggtgc
      421 gcgagatgca cggcagcaac gcggcgggaa cggcgattga aatcaacgtg gaagacgacg
      481 gcagaacctt tttcggggcg catatcgtcg atcccgttgc agtggagaaa gtcaaaacgg
      541 gcgtttacaa gggcttttcc atcggcggca gcgttaccg
//
LOCUS       DAXFHF010000164          573 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXFHF010000164 DAXFHF010000000
VERSION     DAXFHF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.197"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..228
                     /locus_tag="ACFIRM_002141"
     CDS             <1..228
                     /locus_tag="ACFIRM_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029661.1"
                     /translation="LRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADYLKTKEELFA
                     ELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
     gene            233..>573
                     /locus_tag="ACFIRM_002142"
     CDS             233..>573
                     /locus_tag="ACFIRM_002142"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC3029662.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPN"
BASE COUNT          180 a          135 c          148 g          110 t
ORIGIN      
        1 ttgcgcctct accgtttgaa tggtattaac ggtttaaagc ccaaacccaa aggaagaaag
       61 cccgtgaaaa aacagtatcc gccgcaaacg aaaaaagccg actatctgaa aaccaaggaa
      121 gaactgtttg cggaattggc ttaccttaaa gcggaagcgg ctgtcctaaa aaagctcgat
      181 gccttgaaag aagtgcggca gaaagaacgc aactcgtcgc agggttaagg aaatgccatc
      241 cgttgaactg ctgttgggga ttgtcggact gccacgcagc accttctatt accaattggc
      301 cgtccaatcg gcagaaggca aatatgccga tttgaaacga catatccatg atatttatca
      361 acgatataag ggaagatacg gctaccggag gattgcggca gccatccgtc acgcaggaat
      421 accggtcaat cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat
      481 acggcggcgc aaataccgct cgttcaaagg agaagtcggc aaagttgcgc cgaacatcct
      541 gcaacgctgt ttccattcag aaaagccgaa tga
//
LOCUS       DAXFHF010000165          570 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXFHF010000165 DAXFHF010000000
VERSION     DAXFHF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.198"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(245..>570)
                     /locus_tag="ACFIRM_002143"
     CDS             complement(245..>570)
                     /locus_tag="ACFIRM_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3029663.1"
                     /translation="GKDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQKSG
                     IFQDSAQCPAPVTFTITVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRT
                     VSREI"
BASE COUNT          138 a          152 c          159 g          121 t
ORIGIN      
        1 ggaaaggtct tgcgggggct agcccccaca cccccagtct cacttgcgac gccgcgcggg
       61 cagggggaac ggcgcaaaag gcgcgcgcct atcaccctgc ccgcgggcag agtgtattct
      121 tcggcagggc ggcaaggggt atccaaaaag atttataaag acgatgaagc cgtctttaca
      181 aatctttctg gacgtcctcc cccctgcctg aataaacgtt actgtagccc aacggttttg
      241 accgctatat ctctctagat accgtcctga tacagaaaaa ggcggcaacc gcccaagcaa
      301 gggcgagaag catgtacctt agccgttcgg ctatggtaca tgcgttctca aagctgaacg
      361 cgaactgctt gcttgaatca agcacggtta tagtgaacgt aacgggggcg ggacactgtg
      421 cggaatcttg aaagattccg gatttctgaa actctacatt gacggtttca gacggcagat
      481 ttaaatcttc tgccggattg ggctcgggca gcctgtcgca agcgagaatg tcggggaaga
      541 atttgcacaa aagcccgccg tctttgccgt
//
LOCUS       DAXFHF010000166          559 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXFHF010000166 DAXFHF010000000
VERSION     DAXFHF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.201"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT          133 a          117 c          170 g          139 t
ORIGIN      
        1 tgggagaggg ttagagagag ggcagtaagc cgcaaggctt gtattggggc ggttagggaa
       61 ttttttgagc ggggggggaa ttgccgcaac ttgaaaaatg ccctctccca aaccctcccc
      121 cacaggggag ggagcgggtt gcagcagatt ttgcggttgc aggtagtttg aaaagcagat
      181 tggataagca gccgttaatt acaggtcgtc tgaaaaacaa aaagcagcct gcacaagcta
      241 ttttcttttt cctggaatag cgcattgaaa agagtatgat aatggaaaca taaaagattc
      301 cggcaaatgt ccgtgcgccg ttcccctccg tcattcccgc tcaggcggga atccgggtct
      361 gtcgggtttc ggtttctccg atagattctc gctgcgttgg ggtttctaga ttcccacttt
      421 cgtgggaatg acgattcaga tattcctaat agcaagccgc cggcgggcat catagtcctc
      481 tctccctgtg ggagagagtt agagagaggg cagtaagccg caaggcttgt attggggcgg
      541 ttagggaatt ttttgagcg
//
LOCUS       DAXFHF010000167          554 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXFHF010000167 DAXFHF010000000
VERSION     DAXFHF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.203"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            58..444
                     /locus_tag="ACFIRM_002144"
                     /pseudo
     CDS             58..444
                     /locus_tag="ACFIRM_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a          146 c          156 g          108 t
ORIGIN      
        1 cactatatat agaaggtata tatcctagta ggcgtaatgg cagctattac aaaatgtctg
       61 catcaggttc acgaaacgca agtcgaaagc gtcaaacaac tgattgcccg ttttgaccgg
      121 ctgattgacg aatcggacaa acaaatcgac aaccacaccc gcacgcattt tgacggcaaa
      181 gcccaagtgg cggagcaaat caaaggcatc ggttcgataa cgacggctac gctgatggcg
      241 atgctgcccg aattggggcg gctgtcgcac aaacggatag cgggtttggt cggcattgcc
      301 ccgcacccga gggagagcgg ggaaaccaaa ttcaaaagcc gctgcttcgg cggaaggtct
      361 gcggtgcgta aggcgctgta tatggctgcc gcggcagcga cacgttttga accgcttatt
      421 cgggatttcc accaacgcct gctgtatagc gaattaacaa aaaccggtac ggcgttgccc
      481 cgccccggcc caaagggaac gattccctaa ggtgatggag caccgggcgg atcggttccg
      541 taccatttgt accg
//
LOCUS       DAXFHF010000168          529 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXFHF010000168 DAXFHF010000000
VERSION     DAXFHF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.213"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT          117 a          143 c          147 g          122 t
ORIGIN      
        1 ggcgggaatc cagtccgttc ggtttcagtc atttccaaaa attgccgtag cgttaagttt
       61 ctagattccc actttcggag gaatgacgcg gtgcaggttt ccgtgcggac ggattcgtca
      121 ttcccacaca gacaggaatc cggatttgtc cgcgcggaaa cttatgcgcc gtcattcccg
      181 cacaggcggg aattcaggtc tgtcggcata ggaacttatc ggataaaacg gttgccccaa
      241 ccctgcgttc tagattccca ctttcgtggg aatgacgatt caggtattcc taatagcaac
      301 ccgccacaac ccacctacgc ccatcctacg catctgccat acagccctgt tccggagcgc
      361 gcggataagg tgcgatgccg ccggcgggca tcatagtcct ctctccctgt gggagagagc
      421 tagaaagctg caaggcttgt attttagaag actaagggat ttgggaaaga ttgtcgaaat
      481 tcggggagcc ctctccccca cgggggaggg ggcaggttgc ggcagattt
//
LOCUS       DAXFHF010000169          510 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXFHF010000169 DAXFHF010000000
VERSION     DAXFHF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.220"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT          115 a          146 c          156 g           93 t
ORIGIN      
        1 ccattgccgc gcaggcggca atggcgggcc gggcaaacgg ttgcggattg ccgaaaaccg
       61 ctgtaccatg gataagcgcg caagggtaga ataatgcggt aaccctatac attgcatccg
      121 gccaaagggg cgcgttacct ttgcgaacat ccccctttgg cagccgggcg aagggggctt
      181 tgcaaccggc aatccggcgg gcgcgggatc gggcggtttg cccaatcccg ccgtttgcgg
      241 cgcgcctgcc gccgacggta tcccgcgaag caagattgaa gggataaaat atgttccaac
      301 acacagggcg gcacataagg cgccgccctg attcggaagg gcttgcaccc ctcccggata
      361 aagtccgatt ctgccgtccc gaaggacgga tgtccgagcg gcggggtttc aaccgaaaag
      421 gaaatacgat gaataaaact ttaaaaaggc gggttttccg ccataccgcg ctttataccg
      481 ccatcttgat gttttcccat accggcgggg
//
LOCUS       DAXFHF010000170          487 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXFHF010000170 DAXFHF010000000
VERSION     DAXFHF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.227"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>487)
                     /gene="mafA"
                     /locus_tag="ACFIRM_002145"
     CDS             complement(<1..>487)
                     /gene="mafA"
                     /locus_tag="ACFIRM_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3029664.1"
                     /translation="RLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARA
                     AVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYP
                     RYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNETLT
                     T"
BASE COUNT          118 a          120 c          126 g          123 t
ORIGIN      
        1 ttggtcgtca aggtttcatt tcgataatcc cccatcccgc caatatttaa gcccagactg
       61 ctcctacttc cgctaccgtc tgattgggtg cgcgagagtg caggggcatt aagtgtagat
      121 aaagaagtgg ttaaacccgt caaaccgcct gatgttgttt cagcggtggt ttcgtaacgc
      181 ggataggtgt aatcggtgcg gacggcaggg ctgtttatgt attcgccgcg aatcagtgca
      241 tcaatggagt agcgaccccc tgtcaaactg cctgaacctt ggtcgcccat agttgcaatg
      301 tacaatgcaa cttttcgtcc gtgtaatgcc tgtaaatcca tgtctttaac ggcagctctg
      361 gcagaagcgg ccacaagttc ttgttcgacc gcgaagcgtt tgcctccgcc atgcgatgga
      421 atacctgtca gtgtcccgca ggcggataaa ataaaaactg aaaaaagaat aggtatcagc
      481 agccgtg
//
LOCUS       DAXFHF010000171          486 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXFHF010000171 DAXFHF010000000
VERSION     DAXFHF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.228"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>486
                     /locus_tag="ACFIRM_002146"
     CDS             <1..>486
                     /locus_tag="ACFIRM_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3029665.1"
                     /translation="ISTVSDYFRNIRTHSIHPRVSVGYDFGSWRIAADYARYRKWNNS
                     KYSVDIKELERNDNKNSGNHLNIKTQKTEHQENGTFHAVSSLGLSTIYDFDTGSRFKP
                     YIGMRVAYGHVRHQVRSVEQETEIITTYPSNGGGEVSLPSKMPPKSAHHQSNSIRRVG
                     LG"
BASE COUNT          147 a          130 c          103 g          106 t
ORIGIN      
        1 aataagcacg gtaagcgatt atttcagaaa catccgtacg cattccatcc accccagggt
       61 gtcggtcggc tacgacttcg gcagctggag gatagcggca gattatgccc gttacagaaa
      121 atggaacaac agtaaatatt ccgtcgacat aaaagagttg gaaagaaacg ataataaaaa
      181 ttctggcaac catcttaaca taaaaaccca aaagacggaa catcaggaaa acggtacgtt
      241 ccacgccgtt tcttctctcg gcttgtcaac catttacgat ttcgataccg gttcccgctt
      301 caaaccctat atcggcatgc gcgtcgccta cggacacgtc agacatcagg ttcgttcggt
      361 tgaacaagaa actgagatta ttaccactta cccaagcaac ggtggtgggg aagtttctct
      421 tccatcaaaa atgcccccca aatctgccca tcaccaaagc aacagcatcc gccgcgtggg
      481 tctcgg
//
LOCUS       DAXFHF010000172          484 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXFHF010000172 DAXFHF010000000
VERSION     DAXFHF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.230"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..279
                     /locus_tag="ACFIRM_002147"
     CDS             <1..279
                     /locus_tag="ACFIRM_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029666.1"
                     /translation="YYAREDMRQALDGWEAENRILHESGLIEIAKKSAREFVRDADGE
                     PYSQEDWETYLTEDASRIGKDTEAAMNYAIDEREWFALAENIGRLANS"
BASE COUNT          124 a          105 c          158 g           97 t
ORIGIN      
        1 tattacgcgc gggaggatat gcggcaggct ttggacggtt gggaggcgga aaaccgaatc
       61 ctgcacgaat ccgggttgat tgagattgcc aaaaaatcgg cgcgggaatt tgtccgcgat
      121 gcggacggcg aaccttacag ccaggaggat tgggaaacgt accttacgga agatgcttcc
      181 cgtatcggta aagataccga agccgcgatg aactacgcca tagacgagcg ggaatggttc
      241 gcgctggcgg agaacatcgg caggctggca aattcatagc gggcatagga atgccgcgcc
      301 gtcagccggg gagtgggcac ggcggcggtt tttgtagatt ttagccgcaa ataaccccga
      361 cggcgcacgg tctgtaccat ccttagcgga cgcggtgcgc aattaaaacc ttttaaatta
      421 aggaaataga aatgaagaca aggaatattg cgtttaagtt ggcggtattg gcggcggtat
      481 tggc
//
LOCUS       DAXFHF010000173          473 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXFHF010000173 DAXFHF010000000
VERSION     DAXFHF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.233"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            133..>473
                     /locus_tag="ACFIRM_002148"
     CDS             133..>473
                     /locus_tag="ACFIRM_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC3029667.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSK"
BASE COUNT          139 a          112 c          112 g          110 t
ORIGIN      
        1 aactccgccc catccgtaga ggctgataac agtcatgagg ggtatggata cagcgatgaa
       61 gcagtgcgac aacatagaca agggcaacct tgattcacac tgccataacc gcttgctgcc
      121 aaggaaaaca aaatgaattt gcctattcaa aaattcatga tgctgtttgc agcggcaata
      181 tcgttgctgc aaatccccat tagtcatgcg aacggtttgg atgcccgttt gcgcgatgat
      241 atgcaggcaa aacactacga accgggtggc aaataccatc tgtttggtaa tgctcgcggc
      301 agtgttaaaa atcgggtttg cgccgtccaa acatttgatg caactgcggt cggccccata
      361 ctgcctatta cacacgaacg gacagggttt gaaggcatta tcggttatga aacccatttt
      421 tcaggacacg gacacgaagt acacagtccg ttcgataatc atgattcaaa aag
//
LOCUS       DAXFHF010000174          461 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXFHF010000174 DAXFHF010000000
VERSION     DAXFHF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.236"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>461)
                     /locus_tag="ACFIRM_002149"
     CDS             complement(<1..>461)
                     /locus_tag="ACFIRM_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3029668.1"
                     /translation="EAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKFG
                     TRAVPYVGTALLAHDVYQTFKEDIQTRGCRYDPETDKFVKGYEYANCLWYEDERRINR
                     TYGCYGVDSSIMRLMPDRSRFPEVKQLMESQMYRLARPFWNWRKEELNKLSS"
BASE COUNT           92 a          113 c          117 g          139 t
ORIGIN      
        1 gaacttaatt tattcagttc ttctttacgc caattccaaa acggacgtgc cagcctatac
       61 atttggcttt ccatcagttg tttgacttcg gggaatctgc tgcgatcggg cataaggcgc
      121 ataatcgaac tgtcaacgcc gtagcagcca taggttctat taatacgtct ttcgtcttcg
      181 taccaaaggc aattagcata ttcgtagcct tttacaaatt tgtcggtttc gggatcgtat
      241 cggcagcctc gtgtctgtat gtcttctttg aaagtttggt atacgtcgtg ggctaaaagg
      301 gctgttccga catagggaac cgcccttgtg ccgaatttcg cgccttgccg gacaagtttg
      361 ccgacccccg acaatacgcc ggcgcgggat acgctggcgg ttattttggc gttgattcgg
      421 gcttttgcgc ccgtggggat gtgctcgaaa tttgccgctt c
//
LOCUS       DAXFHF010000175          437 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXFHF010000175 DAXFHF010000000
VERSION     DAXFHF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.245"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            181..>437
                     /locus_tag="ACFIRM_002150"
     CDS             181..>437
                     /locus_tag="ACFIRM_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3029669.1"
                     /translation="MNIIGPDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKD"
BASE COUNT          159 a           85 c           87 g          106 t
ORIGIN      
        1 cgtttgaata tagacggctt acgggtcttt gtttcgcgca aagcaagggc taaggcagtc
       61 aggcagcaaa tcccgcaatg tattaaaaca gacgcgtaga aatgccggct gcctttatcc
      121 atcctcaaaa ttgaatatca tcctagccgt atcaaggctg tataaataag gaaaatacca
      181 atgaatataa tcgggccgga catctcaaag gacaccatag acgcaacatt gcataaaaca
      241 aacggaagta tccattacat taaatttaag aataatgatg atggattaaa acagtttaga
      301 ttgtggataa agggaaacag aatcagaaaa gtctatatcg gcatggaggc aacaggcatc
      361 tattacgaaa aggcagcaga tatgctttct tcctactata ccgtttacgt tatcaatccc
      421 ttaaaaatca aggacta
//
LOCUS       DAXFHF010000176          422 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXFHF010000176 DAXFHF010000000
VERSION     DAXFHF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.250"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..304
                     /locus_tag="ACFIRM_002151"
     CDS             <1..304
                     /locus_tag="ACFIRM_002151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029670.1"
                     /translation="YVQADLAYAYEHITRDYPEATGAKKGTTISTVSDYFKNIRTRSV
                     HPRLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
BASE COUNT           98 a          162 c           83 g           79 t
ORIGIN      
        1 gtatgtgcag gcggatctgg cttacgccta cgagcacatc acccgcgatt atcccgaagc
       61 aaccggtgca aaaaaaggca caacaataag cacggtaagc gattatttca aaaacatccg
      121 cacccgctcc gtccaccccc gactcgccct cggctacgat ttcggcggct ggcgcgtctt
      181 ttccgccccc gccgttgccg ccggcctcca ccaatccctt caatattacc gatcccgcca
      241 ttgccgccga ttccgtcaaa tccatcaatt ccgccgaatc acgcctattc cccaaaaacc
      301 ttgatgcggc gggctgaagc ccgccctgca accctctcta tgcaccccct tgcgagcccg
      361 acactacgca acatcttgag aacccatcct gtcaagaata cccgaaccgt cccgatacac
      421 cg
//
LOCUS       DAXFHF010000177          398 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXFHF010000177 DAXFHF010000000
VERSION     DAXFHF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.267"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>398)
                     /locus_tag="ACFIRM_002152"
     CDS             complement(<1..>398)
                     /locus_tag="ACFIRM_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3029671.1"
                     /translation="TFGSRGIPFQFFDALMPSERLEQAMAELVPGLSAHPYLSGVEKA
                     CFMSQAVLWEQALDEGVPYIAVFEDDVLLGEGAEQFLAEDTWLQERFDPDSAFVVRLE
                     TMFMHVLTSPSGVADYGGRAFPLLESEHCGT"
BASE COUNT           91 a          120 c          112 g           75 t
ORIGIN      
        1 gtcccgcagt gttcgctttc caaaagcgga aaggcgcgcc cgccgtagtc cgccacgccg
       61 gagggcgagg tcaggacgtg cataaacatc gtttccaagc ggacgacaaa ggcggaatcg
      121 gggtcaaagc gttcttgcag ccaagtatct tcggcaagga actgctccgc gccttcgccg
      181 agtaagacat catcttcaaa tacggcgata tacggtacgc cttcgtccaa cgcctgttcc
      241 cacaatacgg cttggctcat aaagcaggct ttttccactc cgctcaaata ggggtgcgcc
      301 gacaagccgg ggacgagttc cgccatcgcc tgttccagcc tttcagacgg catcagtgcg
      361 tcgaaaaact ggaacgggat gccgcgactg ccgaaggt
//
LOCUS       DAXFHF010000178          395 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXFHF010000178 DAXFHF010000000
VERSION     DAXFHF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.270"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>395
                     /locus_tag="ACFIRM_002153"
     CDS             <1..>395
                     /locus_tag="ACFIRM_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3029672.1"
                     /translation="RGYRYDPETNKFVKGYEYSNCIWDFVENGIKTYGCYGVDSSIMR
                     LEKDYERFPEVKELMSHQMERIARPFWNKRKEEINKNAALKWRLFPLSHCVFAWNGGG
                     CFVRNGDNLIHRVYFKLERNPKYKEEMDA"
BASE COUNT          130 a           63 c           86 g          116 t
ORIGIN      
        1 cacgaggcta ccgatacgat cccgaaacca acaaatttgt aaaaggctac gaatatagta
       61 attgcatttg ggacttcgta gaaaacggta ttaaaaccta tggctgttat ggcgttgaca
      121 gttctattat gcggcttgaa aaagattacg aaagattccc cgaagttaaa gaactgatgt
      181 cgcatcaaat ggaaaggatt gctcgtcctt tttggaataa acggaaagaa gaaattaata
      241 aaaatgctgc tttaaagtgg cgtctttttc ctttaagtca ttgtgtattt gcttggaacg
      301 gcggcggttg ttttgtacgt aacggtgata atttaattca tcgagtttat tttaaacttg
      361 aacgcaatcc gaaatacaaa gaagaaatgg atgcc
//
LOCUS       DAXFHF010000179          357 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXFHF010000179 DAXFHF010000000
VERSION     DAXFHF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.303"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(49..>357)
                     /locus_tag="ACFIRM_002154"
     CDS             complement(49..>357)
                     /locus_tag="ACFIRM_002154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029673.1"
                     /translation="YMAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNIILCPAEKFL
                     TLKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANFLKLGNPYN
                     "
BASE COUNT          122 a           54 c           46 g          135 t
ORIGIN      
        1 gtacagaccc gaatagtgtc atgttcttca ctaagagata atttatcttt aattatatgg
       61 attaccgagt tttaaaaaat ttgctgcttt ttctatagca acttgccaag ttttatattt
      121 atcgtaattt ttatcaaaac gtactccccc ttgtttattg gataaaaaag taattatctg
      181 attcatatta aaaatatttc cattatggaa aactcgtttt aaagttaaaa atttttcagc
      241 aggacataaa attatatttg tttcagcata gattattggt tcaccagaaa attcttgtga
      301 tgaatgatat atagggctaa taatttttcc gcctaaataa actccaccag ccatata
//
LOCUS       DAXFHF010000180          339 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXFHF010000180 DAXFHF010000000
VERSION     DAXFHF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.316"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(1..162)
                     /locus_tag="ACFIRM_002155"
     CDS             complement(1..162)
                     /locus_tag="ACFIRM_002155"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029674.1"
                     /translation="MALGTSANASNFGISLGKSSAASGTKGIAVGTSSQATNLSAVAI
                     GTESKAQNK"
BASE COUNT          103 a           86 c           49 g          101 t
ORIGIN      
        1 ctatttattt tgtgccttac tttcagtacc aattgccacc gcacttaaat ttgttgcctg
       61 agaagatgtc cccactgcaa ttcctttcgt accactggca gcactgcttt tccctaagga
      121 aataccaaaa ttcgatgcat tagcagaagt acccaatgcc actcaatttt agatgccgat
      181 gctttatcgt tccatgataa ggagccatta ttaccttctt gcaaagtaga atccgcccca
      241 taagaagaca aagcactcat taacaaaata ctagccgcta tcgcggacga cctatataca
      301 taacattact tcatatcatt tcattcagta tcaatagta
//
LOCUS       DAXFHF010000181          332 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXFHF010000181 DAXFHF010000000
VERSION     DAXFHF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.327"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>332
                     /locus_tag="ACFIRM_002156"
     CDS             <1..>332
                     /locus_tag="ACFIRM_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC3029675.1"
                     /translation="PAARHMQTVDGEMAGGNRPPKSITSEGKANAATYPKLVNQLNEQ
                     NLNNIAAQDPRLSLAIHEGKKNFPIGTATYEEADRLGKIWVGEGARQTSGGGWLSRDG
                     TRQYRPPT"
BASE COUNT          113 a           59 c           88 g           72 t
ORIGIN      
        1 acctgctgcc cgccatatgc agactgtaga tggggagatg gcagggggga atagaccgcc
       61 taaatctata acgtcggaag gaaaagctaa tgctgcaacc tatcctaagt tggttaatca
      121 gctaaatgag caaaacttaa ataacattgc ggctcaagat ccaagattga gtctagctat
      181 tcatgagggt aaaaaaaatt ttccaatagg aactgcaact tatgaagagg cagatagact
      241 aggtaaaatt tgggttggtg agggtgcaag acaaactagt ggaggcggat ggttaagtag
      301 agatggcact cgacaatatc ggccaccaac ag
//
LOCUS       DAXFHF010000182          322 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXFHF010000182 DAXFHF010000000
VERSION     DAXFHF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.338"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>322)
                     /locus_tag="ACFIRM_002157"
     CDS             complement(<1..>322)
                     /locus_tag="ACFIRM_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3029676.1"
                     /translation="VSAQVGNRPIAPMVCQNTVAGVFFEARFQQCLLPALAQKSVIIS
                     DNARFRRMGALRGTAEKLGHKVLPPAPCSPEPNPIEKVWANIKRYLRTVLSDYARFDD
                     ALLS"
BASE COUNT           84 a           89 c           79 g           70 t
ORIGIN      
        1 taggacagta gtgcatcgtc aaatcgggcg taatcagaca aaacggttcg cagataccgc
       61 ttaatattcg cccacacttt ctcaatcggg ttgggctcgg gcgaacaagg tgcgggaggc
      121 aataccttat gtcccaattt ttccgccgtt ccccgtaagg cacccatacg gcgaaatcgc
      181 gcattatctg aaataatcac cgatttttga gccaatgcgg gcagtaggca ttgctgaaac
      241 cgcgcttcaa aaaagacccc ggccaccgta ttttgacaaa ccatcggagc aatcggccgg
      301 ttgccgactt gtgcggacac ca
//
LOCUS       DAXFHF010000183          308 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXFHF010000183 DAXFHF010000000
VERSION     DAXFHF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.357"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..257
                     /locus_tag="ACFIRM_002158"
     CDS             <1..257
                     /locus_tag="ACFIRM_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
                     /protein_id="HFC3029677.1"
                     /translation="KTPSTPVYGNDSEAYAAAKKLGYRKIKERTRNDAAIFKKGKSYI
                     SRDVDSHNGGAWKEASSPKNLNRKETRNGTFDKNLNRIGD"
BASE COUNT          129 a           44 c           62 g           73 t
ORIGIN      
        1 gaaaaacacc atctaccccc gtatatggga atgattcaga agcatatgca gcagcaaaga
       61 aattgggata tagaaaaata aaagaaagaa cgagaaatga tgctgctatt tttaaaaaag
      121 gaaagagtta tatatcacgc gatgtagatt cacataatgg aggggcttgg aaagaagcat
      181 cttcacccaa aaatctgaac agaaaagaaa caagaaacgg tacatttgat aaaaacctaa
      241 atcgtattgg agattaatta ttatgtgcca gttcaaggat tttagaagaa acatcccttg
      301 ttttgaag
//
LOCUS       DAXFHF010000184          273 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXFHF010000184 DAXFHF010000000
VERSION     DAXFHF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.419"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>273
                     /locus_tag="ACFIRM_002159"
     CDS             <1..>273
                     /locus_tag="ACFIRM_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3029678.1"
                     /translation="AVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLAEDTWLEERFDK
                     DSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR"
BASE COUNT           67 a           49 c           76 g           81 t
ORIGIN      
        1 gccgtattgt ggaaacaggc attggacgaa ggcttaccgt atgttgccgt atttgaggat
       61 gatgttctgc ttggcaaaga cgcagaaaag ttccttgccg aagatacttg gttggaagag
      121 cgttttgata aggattccgc ctttatcgtc cgtttggaaa cgatgtttgc gaaagttatt
      181 gtcagaccgg ataaagtcct gaattatgaa aaccggtcat ttcctttgct ggagagcgaa
      241 cattgtggga cggctggcta tatcatttcg cgt
//
LOCUS       DAXFHF010000185          270 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.424, whole genome shotgun sequence.
ACCESSION   DAXFHF010000185 DAXFHF010000000
VERSION     DAXFHF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.424"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>270
                     /locus_tag="ACFIRM_002160"
     CDS             <1..>270
                     /locus_tag="ACFIRM_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC3029679.1"
                     /translation="SNLIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIK
                     NRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAISEQIKKQTDNNHY"
BASE COUNT          128 a           56 c           37 g           49 t
ORIGIN      
        1 tcaaacctga tagcagatta cataaaaagg catcaagata cattgatacc gtatcagata
       61 cccaaaaaca aagcactgca aaaactgatt aatcttaaaa accaattaca gcaacagcag
      121 aagcaaatta aaaaccgtct tcatagcact gaagaagact tcataaggaa catacatcaa
      181 gacttgatag ataccataca ggacaagatg gaacaggtaa aaatagccat atccgaacaa
      241 atcaaaaaac aaacggacaa taaccattac
//
LOCUS       DAXFHF010000186          262 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.443, whole genome shotgun sequence.
ACCESSION   DAXFHF010000186 DAXFHF010000000
VERSION     DAXFHF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.443"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            1..211
                     /locus_tag="ACFIRM_002161"
                     /pseudo
     CDS             1..211
                     /locus_tag="ACFIRM_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
BASE COUNT           66 a           62 c           78 g           56 t
ORIGIN      
        1 attaacacct gccgtaaata cggcgcaaaa ctggctgttg taacagccgc ccccgctggc
       61 tttggcggca caggcaaacg cagcgttgcc cgaaacggca aaaaacgctt tggaagccgc
      121 aaaagcggac ggtatggaag ccggttggat tgtagtgggc gttttcgccg cgctttttgt
      181 attttccatc gttaagaggg tgatgaagta aggcggcatg tactgccaag tcggaaataa
      241 atgtcttgag aagcaccggg cc
//
LOCUS       DAXFHF010000187          258 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.453, whole genome shotgun sequence.
ACCESSION   DAXFHF010000187 DAXFHF010000000
VERSION     DAXFHF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.453"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT           95 a           72 c           59 g           32 t
ORIGIN      
        1 gaccaagccc cgaagaaatc aaataccggc aaagcccgga aaccgccgaa acacggcgga
       61 tgaccgaaaa acaggcagaa ggccacatta aaagcattat cagataacgc cgccgcgcaa
      121 ggcataaaac agcgcgagac cgatagacga aacgaccttt aacaggagac cgaaaatgtc
      181 attgatttta agtgtgaaag acgaaagcaa tttcaaacca tgcccggcag gcagccatca
      241 cgccacctgc atccgcat
//
LOCUS       DAXFHF010000188          253 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.467, whole genome shotgun sequence.
ACCESSION   DAXFHF010000188 DAXFHF010000000
VERSION     DAXFHF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.467"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..242
                     /locus_tag="ACFIRM_002162"
     CDS             <1..242
                     /locus_tag="ACFIRM_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3029680.1"
                     /translation="GNGLVQSLSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTESE
                     AALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
BASE COUNT           78 a           55 c           59 g           61 t
ORIGIN      
        1 aaggcaacgg tctggttcag agtctgtccc gcaagggaaa ctgcttggac aatgcggcaa
       61 tggaaagttt cttcggaacg ttgaaatcgg aatgtttcca tacgtgcaaa tatgattccg
      121 ttaccgaatc ggaagcggca ctgcacgaat atatccgtta ctacaacaac gatagaatca
      181 agttgaaatt aaaaggactg agccctgttc agtacagaat tcagtccctg aaagccgctt
      241 gattaaactg tcc
//
LOCUS       DAXFHF010000189          249 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.483, whole genome shotgun sequence.
ACCESSION   DAXFHF010000189 DAXFHF010000000
VERSION     DAXFHF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.483"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>249
                     /locus_tag="ACFIRM_002163"
     CDS             <1..>249
                     /locus_tag="ACFIRM_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HFC3029681.1"
                     /translation="YGQCKNTGQTAATFNLSRNTLYLRIRLKKQTGSLKHQVTGLNAA
                     KSDRQKPARYVGRHPDACLHEIAKHFDCTAAAVCHAPKQ"
BASE COUNT           78 a           71 c           54 g           46 t
ORIGIN      
        1 tacggacaat gcaaaaacac cggccaaacc gcagcaacgt ttaacttgtc aagaaacacg
       61 ctttacctgc ggattcgcct taaaaaacaa acaggcagcc taaaacatca agttaccggc
      121 ctaaatgccg ccaaatcgga taggcaaaaa ccggctcgat atgtcgggcg gcatccggat
      181 gcctgtctgc atgaaatcgc caaacatttt gattgtacgg cagccgccgt ttgccatgcg
      241 cccaaacag
//
LOCUS       DAXFHF010000190          249 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.484, whole genome shotgun sequence.
ACCESSION   DAXFHF010000190 DAXFHF010000000
VERSION     DAXFHF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.484"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>249)
                     /locus_tag="ACFIRM_002164"
     CDS             complement(<1..>249)
                     /locus_tag="ACFIRM_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC3029682.1"
                     /translation="YQLHRTGSEIHPADGYDGPQGGGYPEPQGARDIYSYHIKGTSTK
                     TKINTVPQAPFSDRWLKENAGAASGFLSRADEAGKLIWE"
BASE COUNT           46 a           62 c           63 g           78 t
ORIGIN      
        1 ttcccatatc agttttcctg cttcatccgc acggctgaga aaaccggaag cggcaccggc
       61 attttctttt agccagcggt ctgaaaaagg ggcttgcgga acagtgttta tctttgtttt
      121 ggttgaagtt cctttgatat ggtagctgta tatatccctt gccccttgtg gttccggata
      181 accgccgcct tgaggcccgt catatccgtc tgcgggatgt atttccgacc ctgtccgatg
      241 aagttggta
//
LOCUS       DAXFHF010000191          246 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.490, whole genome shotgun sequence.
ACCESSION   DAXFHF010000191 DAXFHF010000000
VERSION     DAXFHF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.490"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>246
                     /locus_tag="ACFIRM_002165"
     CDS             <1..>246
                     /locus_tag="ACFIRM_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFC3029683.1"
                     /translation="ISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTP
                     ISQREAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGP"
BASE COUNT           75 a           40 c           67 g           64 t
ORIGIN      
        1 attagtgaaa gaaagtcata tcctggttat gtgttagttg agatggaaat gacagatgac
       61 tcttggcatc ttgtaaaaag cactccccgt gtttctggtt ttattggagg gagggctaat
      121 agacccacgc cgattagtca gagagaggct gaaattattt tacagcaggt tcagaccggc
      181 atagagaagc cgaaaccaaa agttgaattt gaggtcggtc aacaggttcg tgtaaatgaa
      241 ggaccg
//
LOCUS       DAXFHF010000192          243 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.495, whole genome shotgun sequence.
ACCESSION   DAXFHF010000192 DAXFHF010000000
VERSION     DAXFHF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.495"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            129..>243
                     /locus_tag="ACFIRM_002166"
     CDS             129..>243
                     /locus_tag="ACFIRM_002166"
                     /inference="COORDINATES: protein motif:HMM:NF020615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFC3029684.1"
                     /translation="MKKIPLNEYVEQHGQAKTAKEIGVTQGAISKALRSGRA"
BASE COUNT           92 a           35 c           45 g           71 t
ORIGIN      
        1 ctccttcgat aatttaaaca tattaaataa tttaaatata ttactgtaag taataataat
       61 caaataaccg caagtattga cttttgaatt acttgcggta ataatatctc tattatttgg
      121 agtaatttat gaagaaaatt ccgctaaatg agtatgttga gcagcacgga caggcgaaaa
      181 cagcaaaaga aatcggcgtt actcaaggag caatttcaaa agcattgagg tctgggcggg
      241 caa
//
LOCUS       DAXFHF010000193          243 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.497, whole genome shotgun sequence.
ACCESSION   DAXFHF010000193 DAXFHF010000000
VERSION     DAXFHF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.497"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT           54 a           63 c           63 g           63 t
ORIGIN      
        1 ggcgaaccgg ttccgtccca tttgtactgc ctgcggcttc gccgccttgt cctgattttg
       61 ttaatccact atatgaatcg tcattcccgc gcaggcggga atccagacat tcaatgctaa
      121 gggaatttat cgggaatgac tgaaactcaa aaaaccggat tcccactttc gtgggaatga
      181 ggtggtgcag gtttccgtac ggatggattc gtcattcccg cgcaggcggg aatccagaca
      241 ttc
//
LOCUS       DAXFHF010000194          236 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.516, whole genome shotgun sequence.
ACCESSION   DAXFHF010000194 DAXFHF010000000
VERSION     DAXFHF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.516"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            36..>236
                     /locus_tag="ACFIRM_002167"
     CDS             36..>236
                     /locus_tag="ACFIRM_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFC3029685.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGANLADIVRFA"
BASE COUNT           54 a           48 c           57 g           77 t
ORIGIN      
        1 tcccatctct cttttctgat tcaacaagga aatctatgga ttatctgcaa aacctgtctt
       61 tgggcttgac aaaaaagctg cccgttatac tgcaaacaga agtagcggag tgtggcttgg
      121 catgtctagc ggctgtggcc ggattttatg gtttctatac ggatttgcgc gcactgcgtt
      181 caaaatactg tctgtcactt aagggtgcga atttggcaga tattgttcgt tttgct
//
LOCUS       DAXFHF010000195          224 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.552, whole genome shotgun sequence.
ACCESSION   DAXFHF010000195 DAXFHF010000000
VERSION     DAXFHF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.552"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>224
                     /locus_tag="ACFIRM_002168"
     CDS             <1..>224
                     /locus_tag="ACFIRM_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3029686.1"
                     /translation="GEKYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQAL
                     LSFLITCAEFALCDKANCNFALHPSYKIQD"
BASE COUNT           73 a           39 c           47 g           65 t
ORIGIN      
        1 caggcgaaaa atacatcggc ggcaactttg tcttttctga tccaccaagc tatatagttc
       61 gttttattga tagcgaattg gatgatatga aagacgaaag tggtgaaatt gccgaaggag
      121 caaaacaggc attgcttagt tttctaataa cctgtgcaga attcgcgcta tgtgataagg
      181 ctaactgtaa ttttgcacta catccaagct ataaaattca agat
//
LOCUS       DAXFHF010000196          214 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.578, whole genome shotgun sequence.
ACCESSION   DAXFHF010000196 DAXFHF010000000
VERSION     DAXFHF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.578"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
BASE COUNT           57 a           53 c           35 g           69 t
ORIGIN      
        1 ataacaaaaa cacttaatgg cggttggttt tatacctatc taagtttttg tgtcgtgcat
       61 atctgaaaag tgtattccgt ttcagtttgt aagctgtggc attctgaggc ctttgcaaaa
      121 aagcccttcc ttcgacagcc gaaacccaaa cacaggtttt cagctgtttt cgccccaaat
      181 acctcctaat tttacccgaa tatcccctta atcc
//
LOCUS       DAXFHF010000197          210 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.592, whole genome shotgun sequence.
ACCESSION   DAXFHF010000197 DAXFHF010000000
VERSION     DAXFHF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.592"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            <1..>210
                     /locus_tag="ACFIRM_002169"
     CDS             <1..>210
                     /locus_tag="ACFIRM_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3029687.1"
                     /translation="IGLPAPYVKPDTSISPTGTIQDRIRWTKSKFPTEKSLNGHFKAH
                     GKEFGDITIEDYQKMASDLLSKQTS"
BASE COUNT           75 a           45 c           35 g           55 t
ORIGIN      
        1 atatcggctt acctgcccca tatgttaaac ctgatacatc tatttctccg acaggaacaa
       61 ttcaagaccg catcagatgg acaaaatcca agtttcctac tgagaaatct ttaaatggac
      121 atttcaaagc tcatggaaaa gaatttggcg atataaccat tgaagactac caaaaaatgg
      181 cgtctgattt gttatcaaaa cagacatcgg
//
LOCUS       DAXFHF010000198          202 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain DONV46_1122
            SAMEA2738426-rid23297433.denovo.621, whole genome shotgun sequence.
ACCESSION   DAXFHF010000198 DAXFHF010000000
VERSION     DAXFHF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2738426
            Sequence Read Archive: ERR779760
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002374682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002374682.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 03:15:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,170
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 116 of 195
            Pseudo Genes (incomplete)         :: 89 of 195
            Pseudo Genes (internal stop)      :: 40 of 195
            Pseudo Genes (multiple problems)  :: 48 of 195
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2738426-rid23297433.denovo.621"
                     /strain="DONV46_1122"
                     /db_xref="taxon:485"
     gene            complement(<1..>202)
                     /locus_tag="ACFIRM_002170"
     CDS             complement(<1..>202)
                     /locus_tag="ACFIRM_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3029688.1"
                     /translation="AVLPGQWHSLSDPELEHSLITRIGFNLFCRFDGPGIPGCSTLCR
                     YRKFRYARAAYFGLLKVGAQSHL"
BASE COUNT           43 a           49 c           71 g           39 t
ORIGIN      
        1 aggtggcttt gcgcacccac tttgagcagc ccgaaatagg ctgcccgcgc atagcggaat
       61 ttacggtagc ggcataaggt gctgcaaccg gggatgcccg gtccgtcaaa acggcaaaac
      121 aggttgaaac cgatgcgggt gatgaggctg tgttcgagtt cgggatcgga gaggctgtgc
      181 cattgtccgg gcaggacggc tt
//
